LOCUS       DASHRQ010000001        99410 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.001, whole genome shotgun sequence.
ACCESSION   DASHRQ010000001 DASHRQ010000000
VERSION     DASHRQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 99410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 99410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.001"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            10..588
                     /locus_tag="U2M88_000001"
     CDS             10..588
                     /locus_tag="U2M88_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372420.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(828..911)
                     /locus_tag="U2M88_000002"
     tRNA            complement(828..911)
                     /locus_tag="U2M88_000002"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(875..877),aa:Leu,seq:gag)
     gene            966..1145
                     /locus_tag="U2M88_000003"
     CDS             966..1145
                     /locus_tag="U2M88_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372421.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            1294..2511
                     /locus_tag="U2M88_000004"
     CDS             1294..2511
                     /locus_tag="U2M88_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEM8372422.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2695..3531
                     /locus_tag="U2M88_000005"
     CDS             2695..3531
                     /locus_tag="U2M88_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8372423.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3607..4755
                     /gene="nagA"
                     /locus_tag="U2M88_000006"
     CDS             3607..4755
                     /gene="nagA"
                     /locus_tag="U2M88_000006"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEM8372424.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4977..6035
                     /locus_tag="U2M88_000007"
     CDS             4977..6035
                     /locus_tag="U2M88_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEM8372425.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6039..7073
                     /locus_tag="U2M88_000008"
     CDS             6039..7073
                     /locus_tag="U2M88_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEM8372426.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7175..7891)
                     /locus_tag="U2M88_000009"
     CDS             complement(7175..7891)
                     /locus_tag="U2M88_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEM8372427.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7860..8645)
                     /locus_tag="U2M88_000010"
     CDS             complement(7860..8645)
                     /locus_tag="U2M88_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEM8372428.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8636..9385)
                     /locus_tag="U2M88_000011"
     CDS             complement(8636..9385)
                     /locus_tag="U2M88_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEM8372429.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9683..10207
                     /locus_tag="U2M88_000012"
     CDS             9683..10207
                     /locus_tag="U2M88_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEM8372430.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10223..11722
                     /locus_tag="U2M88_000013"
     CDS             10223..11722
                     /locus_tag="U2M88_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEM8372431.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11810..12412)
                     /locus_tag="U2M88_000014"
     CDS             complement(11810..12412)
                     /locus_tag="U2M88_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEM8372432.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12516..13472
                     /locus_tag="U2M88_000015"
     CDS             12516..13472
                     /locus_tag="U2M88_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEM8372433.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13777..14733)
                     /locus_tag="U2M88_000016"
     CDS             complement(13777..14733)
                     /locus_tag="U2M88_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEM8372434.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14907..15467
                     /locus_tag="U2M88_000017"
     CDS             14907..15467
                     /locus_tag="U2M88_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372435.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15460..16284
                     /locus_tag="U2M88_000018"
     CDS             15460..16284
                     /locus_tag="U2M88_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEM8372436.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRYVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16294..16911
                     /locus_tag="U2M88_000019"
     CDS             16294..16911
                     /locus_tag="U2M88_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEM8372437.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16929..17612
                     /locus_tag="U2M88_000020"
     CDS             16929..17612
                     /locus_tag="U2M88_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEM8372438.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18295..19578
                     /locus_tag="U2M88_000021"
     CDS             18295..19578
                     /locus_tag="U2M88_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM8372439.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19594..20481
                     /locus_tag="U2M88_000022"
     CDS             19594..20481
                     /locus_tag="U2M88_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8372440.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20498..21334
                     /locus_tag="U2M88_000023"
     CDS             20498..21334
                     /locus_tag="U2M88_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM8372441.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21337..21960
                     /locus_tag="U2M88_000024"
     CDS             21337..21960
                     /locus_tag="U2M88_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372442.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21957..23249
                     /locus_tag="U2M88_000025"
     CDS             21957..23249
                     /locus_tag="U2M88_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEM8372443.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23608..23705
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23821..24402
                     /locus_tag="U2M88_000026"
     CDS             23821..24402
                     /locus_tag="U2M88_000026"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEM8372444.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24405..25712
                     /locus_tag="U2M88_000027"
     CDS             24405..25712
                     /locus_tag="U2M88_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEM8372445.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26129..26617
                     /gene="purE"
                     /locus_tag="U2M88_000028"
     CDS             26129..26617
                     /gene="purE"
                     /locus_tag="U2M88_000028"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEM8372446.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26610..27734
                     /gene="purK"
                     /locus_tag="U2M88_000029"
     CDS             26610..27734
                     /gene="purK"
                     /locus_tag="U2M88_000029"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEM8372447.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSIEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27747..29042
                     /gene="purB"
                     /locus_tag="U2M88_000030"
     CDS             27747..29042
                     /gene="purB"
                     /locus_tag="U2M88_000030"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEM8372448.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29222..29599)
                     /locus_tag="U2M88_000031"
     CDS             complement(29222..29599)
                     /locus_tag="U2M88_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372449.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29855..30583
                     /locus_tag="U2M88_000032"
     CDS             29855..30583
                     /locus_tag="U2M88_000032"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEM8372450.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30583..30837
                     /gene="purS"
                     /locus_tag="U2M88_000033"
     CDS             30583..30837
                     /gene="purS"
                     /locus_tag="U2M88_000033"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEM8372451.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30839..31510
                     /gene="purQ"
                     /locus_tag="U2M88_000034"
     CDS             30839..31510
                     /gene="purQ"
                     /locus_tag="U2M88_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEM8372452.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31511..33733
                     /gene="purL"
                     /locus_tag="U2M88_000035"
     CDS             31511..33733
                     /gene="purL"
                     /locus_tag="U2M88_000035"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEM8372453.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33718..35157
                     /locus_tag="U2M88_000036"
     CDS             33718..35157
                     /locus_tag="U2M88_000036"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEM8372454.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35180..36232
                     /locus_tag="U2M88_000037"
     CDS             35180..36232
                     /locus_tag="U2M88_000037"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEM8372455.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            36229..36807
                     /gene="purN"
                     /locus_tag="U2M88_000038"
     CDS             36229..36807
                     /gene="purN"
                     /locus_tag="U2M88_000038"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEM8372456.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36804..38339
                     /gene="purH"
                     /locus_tag="U2M88_000039"
     CDS             36804..38339
                     /gene="purH"
                     /locus_tag="U2M88_000039"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEM8372457.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38355..39629
                     /gene="purD"
                     /locus_tag="U2M88_000040"
     CDS             38355..39629
                     /gene="purD"
                     /locus_tag="U2M88_000040"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEM8372458.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39751..40665
                     /locus_tag="U2M88_000041"
     CDS             39751..40665
                     /locus_tag="U2M88_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEM8372459.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40798..42363)
                     /locus_tag="U2M88_000042"
     CDS             complement(40798..42363)
                     /locus_tag="U2M88_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEM8372460.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSYFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42356..43807)
                     /locus_tag="U2M88_000043"
     CDS             complement(42356..43807)
                     /locus_tag="U2M88_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEM8372461.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43821..44837)
                     /locus_tag="U2M88_000044"
     CDS             complement(43821..44837)
                     /locus_tag="U2M88_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM8372462.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45057..46145
                     /locus_tag="U2M88_000045"
     CDS             45057..46145
                     /locus_tag="U2M88_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEM8372463.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46199..47725
                     /locus_tag="U2M88_000046"
     CDS             46199..47725
                     /locus_tag="U2M88_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEM8372464.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47727..48932
                     /locus_tag="U2M88_000047"
     CDS             47727..48932
                     /locus_tag="U2M88_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8372465.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49189..50166
                     /gene="guaC"
                     /locus_tag="U2M88_000048"
     CDS             49189..50166
                     /gene="guaC"
                     /locus_tag="U2M88_000048"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEM8372466.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50430..51854
                     /locus_tag="U2M88_000049"
     CDS             50430..51854
                     /locus_tag="U2M88_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEM8372467.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52315..53424
                     /gene="pdhA"
                     /locus_tag="U2M88_000050"
     CDS             52315..53424
                     /gene="pdhA"
                     /locus_tag="U2M88_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEM8372468.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53427..54404
                     /locus_tag="U2M88_000051"
     CDS             53427..54404
                     /locus_tag="U2M88_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEM8372469.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            54429..56072
                     /locus_tag="U2M88_000052"
     CDS             54429..56072
                     /locus_tag="U2M88_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEM8372470.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            56080..57486
                     /gene="lpdA"
                     /locus_tag="U2M88_000053"
     CDS             56080..57486
                     /gene="lpdA"
                     /locus_tag="U2M88_000053"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEM8372471.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            57648..57923
                     /locus_tag="U2M88_000054"
     CDS             57648..57923
                     /locus_tag="U2M88_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEM8372472.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            57929..58696
                     /locus_tag="U2M88_000055"
     CDS             57929..58696
                     /locus_tag="U2M88_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEM8372473.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            58864..60693
                     /gene="typA"
                     /locus_tag="U2M88_000056"
     CDS             58864..60693
                     /gene="typA"
                     /locus_tag="U2M88_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEM8372474.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            60954..62726
                     /gene="recQ"
                     /locus_tag="U2M88_000057"
     CDS             60954..62726
                     /gene="recQ"
                     /locus_tag="U2M88_000057"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEM8372475.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            62840..63142
                     /locus_tag="U2M88_000058"
     CDS             62840..63142
                     /locus_tag="U2M88_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEM8372476.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            63145..64308
                     /locus_tag="U2M88_000059"
     CDS             63145..64308
                     /locus_tag="U2M88_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEM8372477.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            64334..67762
                     /locus_tag="U2M88_000060"
     CDS             64334..67762
                     /locus_tag="U2M88_000060"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEM8372478.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            67783..68907
                     /locus_tag="U2M88_000061"
     CDS             67783..68907
                     /locus_tag="U2M88_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372479.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            68897..69334
                     /locus_tag="U2M88_000062"
     CDS             68897..69334
                     /locus_tag="U2M88_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEM8372480.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            69318..69656
                     /locus_tag="U2M88_000063"
     CDS             69318..69656
                     /locus_tag="U2M88_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEM8372481.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            69775..70335
                     /gene="rsmD"
                     /locus_tag="U2M88_000064"
     CDS             69775..70335
                     /gene="rsmD"
                     /locus_tag="U2M88_000064"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEM8372482.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            70332..70823
                     /gene="coaD"
                     /locus_tag="U2M88_000065"
     CDS             70332..70823
                     /gene="coaD"
                     /locus_tag="U2M88_000065"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEM8372483.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            70813..71856
                     /locus_tag="U2M88_000066"
     CDS             70813..71856
                     /locus_tag="U2M88_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEM8372484.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            72015..72743
                     /locus_tag="U2M88_000067"
     CDS             72015..72743
                     /locus_tag="U2M88_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEM8372485.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            72859..73104
                     /locus_tag="U2M88_000068"
     CDS             72859..73104
                     /locus_tag="U2M88_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEM8372486.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            73101..73319
                     /locus_tag="U2M88_000069"
     CDS             73101..73319
                     /locus_tag="U2M88_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEM8372487.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            73455..73994
                     /locus_tag="U2M88_000070"
     CDS             73455..73994
                     /locus_tag="U2M88_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEM8372488.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            73987..76260
                     /locus_tag="U2M88_000071"
     CDS             73987..76260
                     /locus_tag="U2M88_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEM8372489.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            76298..77344
                     /gene="holA"
                     /locus_tag="U2M88_000072"
     CDS             76298..77344
                     /gene="holA"
                     /locus_tag="U2M88_000072"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEM8372490.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(77433..77687)
                     /locus_tag="U2M88_000073"
     CDS             complement(77433..77687)
                     /locus_tag="U2M88_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEM8372491.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(78087..78356)
                     /locus_tag="U2M88_000074"
     CDS             complement(78087..78356)
                     /locus_tag="U2M88_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372492.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(78826..79281)
                     /locus_tag="U2M88_000075"
     CDS             complement(78826..79281)
                     /locus_tag="U2M88_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEM8372493.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(79419..81347)
                     /locus_tag="U2M88_000076"
     CDS             complement(79419..81347)
                     /locus_tag="U2M88_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEM8372494.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(81544..82377)
                     /locus_tag="U2M88_000077"
     CDS             complement(81544..82377)
                     /locus_tag="U2M88_000077"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEM8372495.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(82569..83273)
                     /locus_tag="U2M88_000078"
     CDS             complement(82569..83273)
                     /locus_tag="U2M88_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEM8372496.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(83296..83922)
                     /locus_tag="U2M88_000079"
     CDS             complement(83296..83922)
                     /locus_tag="U2M88_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEM8372497.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            84248..86623
                     /locus_tag="U2M88_000080"
     CDS             84248..86623
                     /locus_tag="U2M88_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEM8372498.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(86647..87138)
                     /locus_tag="U2M88_000081"
     CDS             complement(86647..87138)
                     /locus_tag="U2M88_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEM8372499.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            87325..88245
                     /locus_tag="U2M88_000082"
     CDS             87325..88245
                     /locus_tag="U2M88_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEM8372500.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            88399..88743
                     /locus_tag="U2M88_000083"
     CDS             88399..88743
                     /locus_tag="U2M88_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEM8372501.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(88933..89433)
                     /locus_tag="U2M88_000084"
     CDS             complement(88933..89433)
                     /locus_tag="U2M88_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8372502.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            89961..90044
                     /locus_tag="U2M88_000085"
     tRNA            89961..90044
                     /locus_tag="U2M88_000085"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:89995..89997,aa:Leu,seq:cag)
     gene            complement(90368..91006)
                     /locus_tag="U2M88_000086"
     CDS             complement(90368..91006)
                     /locus_tag="U2M88_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEM8372503.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            91341..91775
                     /locus_tag="U2M88_000087"
     CDS             91341..91775
                     /locus_tag="U2M88_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEM8372504.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            91798..92118
                     /locus_tag="U2M88_000088"
     CDS             91798..92118
                     /locus_tag="U2M88_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372505.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            92259..92450
                     /locus_tag="U2M88_000089"
     CDS             92259..92450
                     /locus_tag="U2M88_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372506.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(92521..92778)
                     /locus_tag="U2M88_000090"
     CDS             complement(92521..92778)
                     /locus_tag="U2M88_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEM8372507.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            92936..93199
                     /locus_tag="U2M88_000091"
     CDS             92936..93199
                     /locus_tag="U2M88_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372508.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            93223..93411
                     /locus_tag="U2M88_000092"
     CDS             93223..93411
                     /locus_tag="U2M88_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372509.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(93494..93679)
                     /locus_tag="U2M88_000093"
     CDS             complement(93494..93679)
                     /locus_tag="U2M88_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372510.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            93894..94265
                     /locus_tag="U2M88_000094"
     CDS             93894..94265
                     /locus_tag="U2M88_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEM8372511.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(94415..94487)
                     /locus_tag="U2M88_000095"
     tRNA            complement(94415..94487)
                     /locus_tag="U2M88_000095"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(94452..94454),aa:Gln,seq:ctg)
     gene            complement(94533..94961)
                     /locus_tag="U2M88_000096"
     CDS             complement(94533..94961)
                     /locus_tag="U2M88_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372512.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(95021..95779)
                     /locus_tag="U2M88_000097"
     CDS             complement(95021..95779)
                     /locus_tag="U2M88_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEM8372513.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            96074..96271
                     /locus_tag="U2M88_000098"
     CDS             96074..96271
                     /locus_tag="U2M88_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372514.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            96377..96859
                     /locus_tag="U2M88_000099"
     CDS             96377..96859
                     /locus_tag="U2M88_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372515.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(96917..97513)
                     /locus_tag="U2M88_000100"
     CDS             complement(96917..97513)
                     /locus_tag="U2M88_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEM8372516.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            97900..99366
                     /locus_tag="U2M88_000101"
     CDS             97900..99366
                     /locus_tag="U2M88_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEM8372517.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT        32262 a        17025 c        21141 g        28982 t
ORIGIN      
        1 tttttagaca tgaatatctt atttttattg ctatttttct taattttcct tctaacatat
       61 aggaaatgtc aaaactatta tgtgcgtata ttgactgcat taccgttttt cttaaacctg
      121 attatttatt ttggtaatac aatgggtcaa agctttactt acgtcaatgg aaataaaagg
      181 gcaatgattt ggagtagtag taacctgaat aatctcttta cagagttagg aacgaaactg
      241 tcactttttt atccaggtac ttggatagct actttagtag tcttggcatt attactatgt
      301 ttaattgttg gaatttattt aagttttgac aataagaaaa cttctatttt tttagttatt
      361 ttgatgataa tgggattttg ttcaaggtta attatgggat tttctcccac agtatgggct
      421 tccggaatgc ggacgtacta tatactttat gttgttatcg ccatcttagt actaatggca
      481 gttaaagagc taatgaaaag catgagtgta caaaaaaatg aatttatgca atttggatta
      541 accgtgcttg gtatttgtac ctttataata acggtgataa acagataata tacaaaaaat
      601 ccctcgcttt agtattaggt gagggatttt actcgttttt cgattaattg ttttaacact
      661 tgcaagtttc tacgagtagc ttgtttccga ccttcatata ctcacctcag gctgtttaca
      721 tgattgtacg ttctttttgt aaaggataca gtgatatgta tataatggca taaagaaacg
      781 ttcaaacaaa aaaactcgtg aagcaaaatt aacttcacga gttgtagtgc agatgatggg
      841 actcgaaccc acacgagcta acgctcacat gaccctcaat catgcttgtc tgccaattcc
      901 aacacatctg cgtaataata acacactaat gatagcgagg ttttacatag ttgtcaagct
      961 caaaaatggg aggacttatt aagatggaga aatatgacgg agaattttca ggactgggaa
     1021 tgatcttagg tgtattgatt ggacttgcat tcggacgttt tttgcttggt tttatgctag
     1081 gaatcatttg tggaattgca atggattggg cagcgaatct atggaatgac tatcatgaca
     1141 attaagttct caacgcttgt ttgtgttttt ttaatactta cgtttttcct aaataagtgg
     1201 caaaattttc taggatggag tatgatattg aaatagtaaa taacaaaaaa tttggaaagt
     1261 tctaactttt tgaaatgagg gattggctaa gtgatgagag aatttgaaaa atccaacaaa
     1321 ttagaaggtg tcagttacga tgttcgcggt cccgttctag aagaagcgga gcggatgcaa
     1381 gaagagggca ttagtatttt aaaattgaac acgggaaatc ccgcaccttt tggatttgaa
     1441 gcaccaaacg aagtcattcg tgatttgatc atgaatgccc gtgactcaga aggctactca
     1501 gattccaagg ggatcttttc tgctagaaaa gcaattgagc aatattgcca actgaaacat
     1561 tttccgaatg tgacgatcaa tgatatctac accggcaacg gagtgagcga attgatcacg
     1621 atgtgtatgc aggggttgct ggacaatgga gatgaagtac tggttccaat gccggactat
     1681 ccgttgtgga cggcttctat tgcattagct ggaggcacac ctgttcatta tatttgcgat
     1741 gaagaggcag aatggtatcc tgatattgat gatatcaaat caaaaataac gagtcggaca
     1801 aaagctattg ttatcattaa tcctaataat cctactgggg cactttatcc aaaagaatta
     1861 ttagaagaaa tcgttgaagt agctcgacaa aataacctga ttatttattc cgacgaaatc
     1921 tatgatcgct tagtgatgga tggtctagaa catattccga ttgctacatt agcgcctgat
     1981 ttgttcgttg ttacgctgaa cggtctatcc aaatctcatc gtgtagcagg atttcgagta
     2041 ggctggatgg tcctaagtgg agataagtca cacgtcaaag gctatataga agggctgaat
     2101 atgttgtcct ccatgcgttt gtgttccaat gtattgtctc agcaaattat ccaaaccgcg
     2161 ctaggcggtt atcaaagtgt cgatgagctg ctcctgccag gcggaaggat ctatgaacaa
     2221 agagaatata tctataatgc aatcaatgat attcctggtc tttcagccgt aaaaccaaaa
     2281 gctgcttttt atatattccc aaaaattgat acaaagcgat tcaatatttt ggatgatgaa
     2341 aaattcgttt tggacttttt acatgaacat catattctac ttgtacatgg tggtggattt
     2401 aattggaacc agccagatca ttttcgaatc gtttatcttc ctaaaatgga tgatttgaaa
     2461 caaacagcta agaaaatgag agaattccta gcaacctatc gccaaaaatg attttaaaaa
     2521 ccaaattttt cagtaggagg gccacagaca aacacactga ggctctcttt ctagtggagt
     2581 tttcttaaat aactaaagta tattatttag ttgcaagtct tttcttgaag tttgcgactt
     2641 cctaaagtaa aatacgaata gaatgcgaat acgcatattt tggggaggat acttatggaa
     2701 acgttaatcg agtcctttgg aacgatcatt aaagagattc gaaaagaaca aaaaatgacg
     2761 caaaaaatgc tttcgcatga tatttgttca caaagtgtat taagtcggat cgaaaataat
     2821 gaggaactgc cgaatgtatt ggtgatgatg cagatttgtc aacggctagg ggtaacgatt
     2881 gatcatgtga tgaatctctc tcgaaaaact actcggacaa acgataaatt atttgaactg
     2941 ttggatagtt gtttccaaaa acgtgattat cggaagttag aacaagtact aaaatcttct
     3001 gatatttctg aaaatctgta tcaagcaact gactttcaac gctattatta ttatctcggt
     3061 gtttgcgaat tcacgttgcg tcaaaatatc tctcagtccc tttattattt gaaagaagca
     3121 ttaagctact ctagccaaaa agaaagaatc catgtttctg acacagaaat acaactgatt
     3181 agttgtatcg gaaaaatata tggtgtggca ggaaaaacgg ctgaagcacg ctactatttg
     3241 agtcgaagca tccaactgct gcatcaatca gctgatgaac gatcaaaaag tgaattttcg
     3301 caaatttttt ataattatgg aaatttctta ttccatcaaa atgaaatcga cgaagcgtta
     3361 gaacaggtga atcaaggaat ctattgggct caagagaaga atagctacta ttacctcaat
     3421 gatttatttg tgcttaaaag cttaatatac aagcgtaaag aagaaccaga aagagcagca
     3481 ttctatgaag gcttagcaca agctgtgaaa caaatttcaa aaaatttgta aatggtgtag
     3541 accattcttg acttcttctc taaataagag tactttagag gtagaaaaac aaagggagag
     3601 aatagaatga gaacttttat ctatgctgac aaattctttt tagcgtcagg tgtcaaacat
     3661 gcaggctatc ttgaaattac cgatggactt tttggtgaat acaccaaaga actacctgag
     3721 ggagaagtca acatcattga ccaaagcggc aaatggattg ctcctggttt ggtagataca
     3781 catattcacg gctatatgaa tcatgatgta atggataatg atgcagaagg catcaaagtg
     3841 atgtctgaag gtctgttatc ttgtggagtg acttcatttt taccaacaac attgacgtca
     3901 agcaaagaac gtttaaaaga tgtagctgag acaatcggca aaatgtatca ggaagcacca
     3961 ggagctaaaa tcaaaggaat ctattttgaa ggtcctttct tcacagaaga acacaaaggg
     4021 gcacaaaacc caagttattt cggggatcct gatttggata ctttccatga atggcaagaa
     4081 gcttcaggtg gtttaatcaa aaaaattgct ttagcaccgg aacgtcaagg tgtagaagag
     4141 tttgtcaaac aagtgactga agaaggagta gttgtttctc taggacacag taatgctaca
     4201 ctagaagaag cgcaaaaagc tgttgaagca ggcgcaagtg tatttgtcca tgctttcaat
     4261 ggtatgcgtg gattgaatca tcgcgaacca ggtatggtgg gtgcgttatt atctttagaa
     4321 catgtctttt ctgaattgat ttgtgatggc catcatgtac atccaaatgc tgcgggaatc
     4381 ctaatggaaa aagcaggaca tgatcatgtc gcattaatta cagactgtat gatggccggt
     4441 gggatgcctg atggaaatta caatttaggc gaatttccag tagtagtaaa agaaggaaca
     4501 gcacgtctag aatctggcaa cttagctgga agtatattga aattgaaaga agcaattaaa
     4561 aatgttgtag actggggatt agcaacacca gaacaagcag tcatgatggc tagtctgatt
     4621 ccagcaatca gctgtaaaat cgatgataca tgtggaaaaa tcgctcaagg acgcgatgca
     4681 gactttatcg tattgaaccc tgatatgaca ttggaagcaa cttatctaga tggcgtggaa
     4741 cgtttccacg cataatataa tttaagttaa ccttaaaact aaacttctta agtttggttt
     4801 taaggttttt tttgtgttct agtaaaaatc actttacata agtttcaaaa gtttctttaa
     4861 tttcctattg ttcaataagt aacaatgtaa agataaaatt aaaaatgtta atacgtatta
     4921 gcaaaataaa agcttttttt tattgtgaat aaatgaacat aattaggagg agaaagatga
     4981 acttatttga catttttcga tcgactttga cagatatgtc gattgtcagt gcaatcactt
     5041 caacagtttt tattatatta ttagggtttg tttgtagaaa aaaggggatt ttttctgaat
     5101 ctgttggaaa aattctatcg aaagtcgtct taacagtggc tttgccagca ctggcatttc
     5161 gagctttcat gcaagatatc aatacagaat tactgatgca aggaatcaat gtcttgattt
     5221 ggggattagt tatttatatt attcttattc tagtttcaaa accttttttc ttaagctata
     5281 aaggggataa gcaaacaacg ttgcgtgtcc tgacgatctt tggttctacg acctttttcg
     5341 gaacgcctat cgtcagtgcg atttatggtc ctgtcggcgt gatgtattcc tcaattttca
     5401 atattggcta ccgaatcttt ttgtattctt atggatacat aaaaatgagt ggcttgaaga
     5461 tggaagctaa aaatatcaaa gcgatgttcg tcaatccaat cattatcgct acttttgccg
     5521 gccttttcat ttggctgttc caagggtatc tgccacaagt gtcagttaca ggtgcagaag
     5581 gagagataag ccaagtagcg tttttgcgaa ttgaccagac tgcaccgtgg ctatttcagc
     5641 cgatggatta tttagctggt ttaagttcac cattggcgtg gctagcaatc ggcgcaacat
     5701 taggtgaagt aagcttcaaa aaagctgcat ctgaaaaaac ttcatggtat tacagtctag
     5761 tcaaagtggt atttgtacct gcattgaata tcgtgattct agcagttttg acggcaacga
     5821 atattctacc agtcaatttt gaagcactgg caacaatcgt tatcatgatg gctacaccag
     5881 ctgcaacagt ggcagcagcc tatgctatca gttttgatag ggaggcactt ttagcctcca
     5941 atgcatcctt actgtcaact atttttgctg tggtactgac acctgtatgg atcgttgtat
     6001 tacaaatgat tcatggcata ggtttatttt cataagaaat ggaggaagaa agtatgagaa
     6061 tcgcatgtta cggtgttcgt ccgaacgaga tagatttttt tgaaaaattg aatatttacc
     6121 attatgatct cagtctttat gaagaactgt tgacacatga caatatcgtg tcagcaaagg
     6181 accatgatgc tgtattgttg aggggaaatt gtatagcaga tgagaccaat cttgcaaaaa
     6241 tgcaggaata tgggattcgt tatgttttta cacggactgt gggagtcaat catatcgatc
     6301 tacaagcggc agctgatttt ggtatgacag tagcccgtgt accatcgtat tcgccaaatg
     6361 cgatagcaga gttatctttg acttttgcca tgatgctttt gagaaatact gcgtatacga
     6421 cgatccgaac atctttcaaa gattttcgag tggatgagca aatgtttagt cgtgaaatca
     6481 gaaattgcac agtagggatc ataggaacag gcaggatagg gttgacggaa gctgttttgt
     6541 ttaagggact aggagccaaa gtgatcggct acgatattca tcagacagaa gctgctaaaa
     6601 aagtattgac gttccactcg cttgaagagt tattagccaa aagcgatatt gtcagtattc
     6661 atgtacctca tatcccaggt gaaaacgatc aaatgatcaa tgaaggattt ttagctcaaa
     6721 tgaaaaaagg tagcatcttg attaatacag cccgtggaga gcttcaagat aatgaagcta
     6781 ttttaagagc tttgaaaagc aatcatttag ctggttttgc aaccgatgtt tttgcaaatg
     6841 aaacagaggt ttttttccga tccttcaagc catgggagac gattccagac cctgctatcc
     6901 agcaattagt agaaatgtac cctagagttc ttgtaacccc gcatgtaggt tcaaatacag
     6961 atgaggcatt atcaaatatg atttcgacaa gtttcgagaa ctttcgtgaa ataatagaaa
     7021 ctggaaaaac gaaaaatgag gtatctcttc ctaaagcccg tcaattaaga taagaaaaca
     7081 tggatattcg tcaataaaat gtttaaaaat agtccaagta aacagttgtt acttctgtat
     7141 acttggacta ttttgtatgg gataattcga tgactcaaca gtaatctgtg agctttttcc
     7201 gcccattctt tgtcaattca taacaggtta gcggccggcc gatttccaaa tatttgattc
     7261 tttcgttcaa tatatcttgt tctactaaaa aattcaaata tttcttgacg gatatacgtg
     7321 acagtgagct tttttgagcc agttcttttg ttgaaaaaat agtatcttct tttagaataa
     7381 atgcacttat tttttttaaa gtcagttttg acagaccttt aggcaaatcg atgaatgatg
     7441 gtatttcgtg aatgggagtg gtgtggttga atacttgatc gatcaatttc tgatcggctt
     7501 tttcagtcgt ttggattgtc tgagagaaag caagatactt ttcgatcgcc agctgaaaac
     7561 gttgcaaagt gaacggtttg atcaaataat ctaaggcacc gtagcggatc acattcttta
     7621 tggtttcgat atcatctgca gcagtgatca aaatcactgg gatatcattt ccttgctgtc
     7681 taagccaaga taaaaaatgg ataccacttt cgccaggcat gtatacatca agcagcagca
     7741 gatctgcgtt tttttgtctg aaatattttt ttccttcttc tacatttctg aaactttcga
     7801 ttacgttcat attagggatg ctttccacaa aagtacgatt caattctgca accattggat
     7861 catcttcgat tatcaggaca tttgtcatca ttttgatctt ccttttttgg taattctaga
     7921 tagacgattg taccgccatt tgtttgtttg gaaaattcta aaaatccacc gtggcgtttg
     7981 acgattgaat ggatgagatt caatccaaac ccacgattga ttcccttcgt cgaaaaacct
     8041 agcgtaaata ttttttctga gatttcttct gatatcccga caccgtcatc gcagacggtc
     8101 acgatgaata cttcagcttc tgtgtcatag tttaaatgaa tccggacctg tccgtttctt
     8161 tcatgttttt gtagcacggc ttcttttgca ttatcaagta agttccctaa cacttgcaaa
     8221 agtgaatgga tggagtcatc catctccatt ttagggataa aagaatcgct ttctaacagc
     8281 atttcaacag attgttcttt ggcttcgttt attttgccta ataaaaaacc ggcaattgca
     8341 ggtactttga tcagacttga gacttggcca atctcttttt tataattttg atttagctgc
     8401 tggataaaat gggtgacttc ttcatacttc ttcaaatcaa tcaaaccaga aatgacatga
     8461 agtttattca tgaattcatg ggtttgagct ctcatggagt ttatatattg tttcgtcccc
     8521 ccaagctcct ccattaattt cattacttct gatcgatttc gtaaagttgc gactgctcct
     8581 aaaaaacggt cattgctaaa aagaggtgta acgctagaaa tagtttctat cccatttaaa
     8641 tacatggatt gagtccgaac ggtcgtttga tgctcaatcg tctctttaaa caaaacgtca
     8701 tatagattat cactgatttt tttcctactt cgaaatccaa tagctgctct gtttggtaga
     8761 aaatgttttt tgcaggtgta ttgatcaaga ggatagtttt atcagaagag atggctaaga
     8821 ttccttcatc tacttcattt tcgataatcg ttttttcact taactgttct gctatttctt
     8881 gtggttcaag tccaagcagt acttttttca ttttttgggc aagaaaaacg gcgcctatga
     8941 tgcctacgct aagtccaata atcaatgaaa aaacaatcat tttttgtcca tctttgactt
     9001 gattgtcaat cgtgttgttt gttaagccaa cacaaactgc accaataatc tcttgatcat
     9061 aataaatagg gacgaaatag cggaagccga accctaacat tccttctttt tcagagaaaa
     9121 attcttgtcc tccaatcact tttttcacat cttcatgact tgagaaagga agaccgatgt
     9181 aacttgggtc tgcgtgagaa tatcgaatgc cttcagggtc catgacaacg ataaaatcta
     9241 aattcgatgc aatcatgatt tcatttgtta ttccttgaat cgcatgtgtc gggtcaccag
     9301 tctgcagctt tttttggatg agtttatcat tggcaacgat tttagcaatg ctagagattt
     9361 tatctgacac actagatcgg ttcatcgaaa gaataaaatt cctgatcaat atggcagaaa
     9421 caatcaaaga aacgaatgtg gagaaacaaa cgacacggat gatcgtgttc tggagcttta
     9481 atggtttttt cttaaaaaaa ggaaacatag gctacctcga aagaatttat tgtgaaaatt
     9541 ttaacatgaa aacaaaaatt taacaaattg tcaacgataa acacaacctt cctataaaaa
     9601 acaccactga gtcggtaaaa gaagctatac agaattttca aacattggta tgatagacgt
     9661 agaaatggaa ggaggcgtgt atatgatagg gggaatcgca cttgagacga tttattggta
     9721 tacattgctg atttgtgcag gattggcagt cctattgatt atttttggag atgtattcga
     9781 ttttgatggc ccgcttgatc cgatgcttat tatcccttgg ttaactttca caagcttatt
     9841 tggttttcta ggtgagcgtt taacagatca atcatcttta tttttattta ttgctagtgg
     9901 aggcatatcg atagtgttgg tatttttgct taatttctat atattgatgc cgatgaaaca
     9961 tgccgaatca acgatgtcgt cttctgaaaa aactctggaa ggacaagtag ctgttgtgat
    10021 cacaccgatc cctgtacgag gtatgggaga aattcagttg aaaagtgtta ccggttccat
    10081 tagtcgtcca gcttgttttt acagccccca agaacagaca gctgagcggg gaacgaaagt
    10141 attgatcatt gagatcaagg aaagaatctg ttatgtcatg ccttatgaaa gcatgataaa
    10201 agcttaaagg agcgtgtgtt aaatggaaat accagttgtt tttatcgcga tcgcaatcgc
    10261 agctttgatt ttactgatgt tgctgatcgt ctttgtagcg aagtaccaga cagcaaaacc
    10321 ggatgaagcg ttgatcatca gtggaagtta tttaggaagc aaaaatgtgc acattgatga
    10381 aagcaataac aaaatcaaaa ttgtgcgtgg aggtggcgca ttcgtcttgc cagtttttca
    10441 acgttcgaat cggatcagtc tattatcgag taaattggac gtctccaccc ctgaagtgta
    10501 tacggaacaa ggcgtaccgg tgatgtgtga tggtacttcg atcatcaaaa tcggttcatc
    10561 tgtagaagag attgctacag cagcagaaca gtttttaggc aagacaagag aagaactgga
    10621 aaatgaagca agagaagtat tagaaggaca cttgcgctcg attcttggtt ctatgactgt
    10681 agaagaaatc tatcaaaatc gcgataagtt cagtcaaagc gtacaagaag tcgcttctgt
    10741 agaccttgca aaaatggggt tgatcatcgt ttctttcact atcaaagagg tcagagacaa
    10801 aaacggttat cttgactctt tagggaaacc aagaatcgcc caagtcaaaa gagatgcaga
    10861 tatagcagaa gcggaagctc taaaagaaac gcgtatcaaa aaagctcaag cagaaaaaga
    10921 atcacaagca gctgaattac agcgacaaac agagatcgca gaatcattaa aagaaaaaga
    10981 actgaaatta gcaacctaca aacaagaaca agatgttgct aaagcaaaag cagaccaagc
    11041 ctacaattta gaaagtgccc gagcacaaca acaagtaatc gaacaggaaa tgcagattaa
    11101 aatcgtagaa cgtcaaaaac aaatcgaact ggaagaaaaa gaaatcacac gtcgtgaaaa
    11161 gcaatatgat tcggaagtga agaaaaaagc agatgccgat cggtacgcta aagaacaaga
    11221 agcacaagct caaaaagtca aagaagtgac tgaagccgaa gcagaacgat tccgtgtgga
    11281 agcactggca gaagcggaag ccaataagac acgtttagct ggacaagcag aagccgaagc
    11341 agcactagca aaaggaaaag cagaagccga agcaaaacaa aagatcgcca atgccttcaa
    11401 ggaatatggc gaagcagctg tcttgagtat ggttatcgat atgctaccgc aattgatgcg
    11461 cgaagcagca cagccgctag gaaatatcga aaaaatctct gttgtcgata caggaagcag
    11521 ttcaggagaa accagtggag cgaaccgagt gacaaattat gccacaaatt tgctttcttc
    11581 tactcaagaa acactaaaag cgacaacagg acttgacttg aaggaaatcg tagagaattt
    11641 gtcgacacgt gggactacta gccaagtaaa tatgtatgaa gcgccaaaag aggcagaaca
    11701 aaactcgatg aaggaagaat aaagtagtac acttcaccgt tcaatcaaaa atggaggaag
    11761 actttttaaa gttcttctct ccattttttt attttattgc aaaagaaaat tagtcctcgc
    11821 tatttttctt ttgtttttgg atcttagctt ctatctcttg taaatttgtt cttagcaatc
    11881 gttctttctt ttggagttcg accaaatctt ctgatagttc ttccttcttt tctgtttcag
    11941 gaggacgaag aaagaaattg ataccgattg caatgaatgt ccctataaaa gtatctagta
    12001 cacgttggat agcatacaaa gcggattcgc cttgagggat gctcaacgca atcagcaaca
    12061 acgtagctat tgcggaaatg ataccggaat tattgttaat accatctgac acgacgatca
    12121 caataataac tagaatagga agcaagatca attctaccaa aaaatcattg tgaaaatagc
    12181 ttttgacaaa aaaataaatg atagctaatg taccacctaa tgtattgccg atgatcctag
    12241 actttccaaa agaaacggtt gtagtcaagt cctgacgcaa agaaaaaaca gcagataatg
    12301 cagcaatcat cgggattcct cgttgagtca aatgaaaaaa caagatacat aaaaaaacag
    12361 ctaaggcact tttgatcgta cgcataccta gtcgaaaacg gccaaattgc atagagattc
    12421 tcctttttgg tctttttact atcataccta aacttttgtc tgatagctag tttttcatta
    12481 ttcaagtata attaaaataa aaaaggggga agtaaatgcg tataggaatg cgaacagtca
    12541 aaacagtgat cagtgtcttc ttttcgatgg tcacagcggg tagcttatcg ctgttgtatt
    12601 ggccagctgc agggatcatt gcattattaa gtgtaggaaa taccaagcgc tctacattgc
    12661 gtacgggcat gaatcgaatc gcagccttta tattagctac ggcaacagca ctgttcagtt
    12721 tttttgctgt tgggtatact atttttggct tcagtctttt tttattcctg ttcatcccgc
    12781 ttgcctctcg cttcaaatta accgaaggga tagtagtaaa ttcggttttg gtcactcatt
    12841 atttagtgga ggaaaacatg tcttggcagc tgatcggcaa tgaagcggga ttaatggcga
    12901 ttggattagt atttgctttg cttgcaaatg tttacatgcc agatcgtacg aagcagctga
    12961 aggaaaacca aatacagatc gaaaaagaat ttcgaaatat tttgagacaa atggcagaat
    13021 ttcttttatc agaaaataaa gaagatgtgc agatcaaatg cgaacattta ctgacattca
    13081 ttcgcgaaag ccaagaagat gccagagaac atcaagaaaa ctactggctc agacagccgc
    13141 tatactacga aacttacttc tctatgagaa gagcacaggc caatgtgatt aaagacatgc
    13201 tggaaaatct agagagaatc cagcaaccag cttattatgg aaaacatatc tatggtttat
    13261 tgatttatac tgcagaaaca ttttcagagt caaatgatgg cagacaaatt ttaacacgta
    13321 ttgaagaagt ttatgtattg tatcggcaaa tgcctctgcc gactagtcgc cctgaattcg
    13381 aggatcgagc agaattgttc cagtttttgc agtcttttaa aagttttatt gaaatcaagg
    13441 cagaattctc ccaacaaaaa atccaaaaat agaggttgtg aattcagcta atattcaggt
    13501 caaaaagctg atttcacaac ctcttcttta aacggtgttc tcctgtcaac tcctcggtca
    13561 caagtcacaa tattcaacaa acatgagaag ctgttttttg aaattgctcc ttgtgactcg
    13621 gagctgaacg gtaggctcac aacctctttc ttaaacggtg ttcaaaagct ttcctctgcg
    13681 gtaataagtc cagccaaacg aaaaatagga ggagctattt ttgtttggct gttcttatta
    13741 cttgatgcag aacagctttt tcacaacctt ttaaaattac atatttttcc atagaacttg
    13801 catttgatcc aagatttcga tgacatcttt tgtcaagaag agagagcgat tgggcaagtc
    13861 accttcaatc atgtgtacca tattctttat ttcgtaattc attgcttcag atgtattccc
    13921 gctttcgatg aattcctttg taccatctgt aaataagatc tccgcacgat cagcacgcgg
    13981 gtaatcagca atcgtcacat aaccgttctc aaaggcaacg ataccgactt ttggcatttt
    14041 tgcctgaaaa gtcaagctga cagcagcaag ttcattttct ttgttacgca agatagtgac
    14101 ggattgttcg tccacgcctg tttcaaaagg aaccattgta gaggcaacta cttcaggttg
    14161 agagctcaaa aagaaacgag cgaaagatac agcatacgta ccgatatcca acagagctcc
    14221 tccagctaat tctggattga agaaccggtt tttagggtct ggttctttgt aactgccgaa
    14281 aggtgcttgg atcattttta aagcccccag ttttcctgta tccattattg agcgtaactg
    14341 ctgataaagt ggcatgttga agattgtcat cgcttctgct aagatcaaat tcttttcttc
    14401 tgctaagcgc atagcgtcag ccagttcttt tttattcatc gtgatggctt tttcacatag
    14461 aacgtgtttg cctgcttcca atgctgccaa gatatggtcg atatgctgtc tatttggtac
    14521 agcaatgtac acgatgtcga tttcagcatc gtctaacatt tcttgatagc ttccgtaagc
    14581 ttttggaata ttgtaacggt gagcgaagtt ttccgctttt tctgaagttc tggaagcaac
    14641 tgctgttagt tcgctcgtct cttgattgaa gctttcggca aactcatgtg cgatggtacc
    14701 aagaccaacg attccccatt gatatttttt catctgaaaa cgctccttca tagttggatt
    14761 gagtttcttg tctgtctatt attataacaa aaatcacgaa gaataaagag gagaggactt
    14821 gaaacattaa aaaaaatgaa agcagcgatt taagaggtgc tttttgatca aaacttaagt
    14881 agaatagtta aaaggagggg ataaacgtgc atcaagtata tattgatctt attatgagtg
    14941 tattattaga acagtttgaa actgaaaaaa attttttaga tgaatacttg ctgatcagtc
    15001 cagaacaatg gcaaaaatgg aaagatggcg agaaaaatct gccatctgaa gcgatgcaga
    15061 aagtaaaaaa tcttttcact gattatgaat ggatgctaac gcaaaagatt ttgcgtcaaa
    15121 cgattatttt tcccgaaaaa cgaaacgttg ccgttgcaga gtacaagcaa atcaaaacac
    15181 gcatcgcgca aaaatggctt agcagcaaag cagcaaaagt cgagctgatc cctttgaaaa
    15241 ataatcagca ggctggtaga taccttgact tgaaagtaag tattcaatat gatgaatggg
    15301 gctatgatga tattttgaat tttcgtctgc cagcagatgt tcaaaaacag atcgaaggag
    15361 aaaaaatcga acttctcgat tgggtgaatg aaaatctgga agaaacttac gttcatgagg
    15421 cgaatgggga acgtgaagaa caacgaaagg atttttcaag tgaataaatt tttaggattt
    15481 ggagcagttg tgattatgat cgcagcaatg ggctatgtta tttttagcga tgatgattcg
    15541 catgttcaag ccggcaatca agcagtcgaa cgaacaagca ccaaaacaac tgctagtaaa
    15601 gaaaagaaca gtcaaacaac cagcagcaag gtaaaggaca aagaaacaaa agatcgtacg
    15661 tccaaacaat tgccagatgt taaaacagac gattggaagc tagttttggt tggtccagat
    15721 cacaaattag aaaaagaaat tgatgagtct actcaattgg catcattaag taacggatac
    15781 ttgatcgaca aacgcattgc tccagaatat gaagcatttg ccaaggctgc tgaagcagct
    15841 ggatttccat tagtgatggt ctcagcttac cggtatgtat catcccaaca acaagttttc
    15901 gaacaaaatg tccaagatgt gatgagtcgt caaggtgtat cagaagagga ggcgacaaag
    15961 attactaaag aaacgattac ggagccagga tacagcgaac accacacagg tctggcagtc
    16021 gatgtcgtcg atcaaaactg gtacaacagt tatccttcta ctgtattgga tgcaagttac
    16081 ggagatcagc ctggagctaa atggatcgca gataatgctg cgcagtatgg atttatcgtt
    16141 cgttatccaa aaggacacga agatataact aagatcacat atgaaccttg gcacttacgt
    16201 tatgtcggta aagaaaacgc tgagtatatc accaaacacc aattgacgct agaagaatat
    16261 ctgaatgaat taaatgaaaa gtaggaattg atcatggcga aaaaaaagtg gaaaaagcaa
    16321 aagcgcaaga atatccgttg gccagcagtg gttgccggtg tagcagtgat actcttagcc
    16381 tttgtttttt ccttgaagaa tctgtcttct ccttttactg ataatcagca agaaaatgaa
    16441 gcgcaatccc atcagcaatt tattgaacgg cttgttccgc atgcccaaga actacaagat
    16501 ggttatggag tgcttcctag tatcatttta ggacaagcta tattagaatc aaattgggga
    16561 aaaagtacct tggctagcaa atacaataat cttttcggga tcaaggctta tggggatcag
    16621 aagaaagtaa gcctggaaac aaaggaattt gttaacgaag aatggataac tatccaagga
    16681 gacttcaaag tgtatgattc atgggaacag tccatggatg accatacaca actgttcgtc
    16741 caaggagtag attggaatcc tgcgctgtat gaaaaagtga ttacggccac caactatcag
    16801 gaagccgcac aagcattgca agatgcaggt tatgcgacag accccggtta tgcgcaaaaa
    16861 atcatccagg tcatcgagac gtatcaattg aatcagtatg accggagata gcaaaggaga
    16921 agtggctgat ggcagaacaa tttacgccag aaatcatgac ggaattgaga aaagatatcg
    16981 taagagtacc ggaaatcatt ttagaagcaa gcgggatcaa gatatttgga aaattgatcc
    17041 gttcgatcat ctttactacg gatattgcga tcatacgtaa tacaaatgcg aatgcagtaa
    17101 ttgctgtgta tccttttaca cctcatccag cgattactaa aagcattatt gaggcagctg
    17161 acattccagt tttctccggt gtaggtggtg gtttgacaca aggtgctcgt tcttcctaca
    17221 tgagcatgtt cgcagaagcg caaggttcgt taggggtcgt tttgaacggt cctacgcctt
    17281 tagagacagt aaaagaagtt tgtcgagtag ttgatattcc agttatcagt acggtaacgt
    17341 cgaaatatac agaaatagat gaaaagttag cagctggtat ttcgattatc aatatcagtg
    17401 caggtaaaga aactgcagaa gcagtacgcc attttcgtaa aaaatatcct gaacttccta
    17461 tcattgcgac aggtggtcca acggaagaaa gtattgctga gaccattcaa gcaggagcaa
    17521 atgccatcac atatacgcca ccaagtagcg gagagctttt tagtcgtaaa atggaaaaat
    17581 atcgggaaac tgagaaaaag aatcatgaat aacaggtgag aatcaaaaga cgaggaagag
    17641 cagatagtgg ctcttcctcg tcttttgtac tccagtacta tttgttcaga attcaaaaaa
    17701 aagagaaatt tttttgtttt tttacctgta atgtacataa gaagagttaa aaccttttgt
    17761 tattattagc attctaacaa aaaaatgtct tgatttattg aagcgctttc tatagaatga
    17821 aaaacgaata aaaactaaaa aaggcacccg acaatttagt agcagctaaa ttgtcggggc
    17881 ttgaaaagtc agcgggaact gaccaatcaa gttgccatag tcaatgcttg tgcatcgacg
    17941 tgtatattgt acctaatttc atacagaatt actagttatt tttctgtttt ttagggaaga
    18001 gcgcacacga agaagcatgc ttatttagct atgtcaagga tattgggcag ttgtctgata
    18061 tccttttttc tttgcgtaat tatcctccca tcattgaagc aagaagtgtt tttagcgatg
    18121 aaagaagaat cagcactatc taatttttct tgaaagtatt gagttgttca tcctaggcag
    18181 aacgataagg caagtcttgg tttttattcc ccttgaaaaa gatcgtttaa taaatatgtt
    18241 ctgtataaaa atacagtata ttgaaaaata acaatggaag tagaggtgca gagaatgaag
    18301 aaaagaatga gaatcatctt agctttaagt gttatgttat tcttcttgac ggcttgtggg
    18361 caagaagatg acaaaaaaat cacgctgaac atggcttggt ggggttcgca agtccgacac
    18421 gatgcaacag taaaagtaat cgagctttat gaagaaaaaa atccccatgt gacgatcgat
    18481 tatgaatttt atgattatga aggatattac acaaaattag gtacactagc agcatcagat
    18541 gatatctggg acattttcca actgaacaat caatttcctc aatacatcaa tcagattgaa
    18601 ccattagatg aatatgtgga gcaagggata attgatattt ctaatgcaga tgaatcctat
    18661 ttagcaacga cgacttacga cgggcaatta gttggactgt ctaacggggt gaattcgttt
    18721 gcgattgctt acaataaaaa tatatttgaa caattaaata tccctgaacc agcttcaaat
    18781 tggacatggg atgaatttga acaaatcagc tatcaaatca ctgaagaatt aggaatctat
    18841 gctatttcaa gattcgaaga ttttatagct ggctgtatta ttcaaatacc acaagttgag
    18901 aaaggattga atttttataa tcgggaagat ctatctaaca gcttgggatt tgaaaatcca
    18961 gactatctaa cagatttttt ccagctgcgt aaaaatctgg tagacaaagg ggcccatcca
    19021 gaaccaggtg gtggagctgc aagtacgacg gatctgtctt ttcaggcggt tagggataga
    19081 gaagcggcaa tggtgtttct atcaagtaac cagttgacag aaatattgtc aggggctcct
    19141 gaagatatgg agattcagtt gattaatcca cctcgcagaa aagcagatgg agaatctggt
    19201 atccctttac gttcttcgca aatgctgagt atggcaaaaa gctcagccca taaagaagaa
    19261 gctgctaagt ttattgattt ctttcaaaac agtgaagaag ccaatgaaat tttaaaagga
    19321 gaacgtggtg taccaatcat gtcgaatatt cgagaacagc tcgctcgtgc agatgatgca
    19381 actttgcaag cgacatttga ttatttagaa atgatttccg atatggacaa cggcgaagtg
    19441 aatgtctttg atagtgcttt gaatccggaa attcaagacc aatacaatct tttagttgaa
    19501 aaagtcattt atgaagaagt aacagcagaa gaagcagcag agagtcttta tgagtttgcg
    19561 tcaaacattc taggatagca gcaggaggaa ggcatgaaaa aagaaaaact aaaacggttc
    19621 ttggatcaag acaatgtggt cggttacgta tttgcttctc cattcatcct aggatttcta
    19681 ttgataacag tgataccgat gatcttgtca ctttattatt cgttcaccga ttatcgtttg
    19741 ggaaatccag tctcatggat cggcttggat aattatataa agatgctgag tgatgcccgt
    19801 tttttgaact ccatccgagt cacttttctc tatgtatttg catctgtacc tgtgaaactg
    19861 atctttggtc tattgattgc ttatctttta acgaggaata tcaaaggaac gactttcttt
    19921 cgttctttgt attatgtgcc tagtttgatc ggtggcagta tttctatcgc tctagtttgg
    19981 cgggagttgt tttctaaaaa cgggttagtg aatattttcc tggaacgatt aggcgcacct
    20041 gcgctttcct ggtttagcga tcaaaaactg gtcatgatcc ctcttgtttt gatgtctgct
    20101 tggcaattcg gttcggcaat gatcatcttt gctgcaggta tcaaagagat cccaggctcg
    20161 tattatgaag cggcagagat cgatggagcg aacaaatggc agacgtttta caagatcacg
    20221 ttacctcttc tttcgccgat cattttgtat aacttggtta tgcagacaat cgcggcgttt
    20281 atgactttta cgcaggcatt tatcattacc caaggtggtc cgaatgatgc cacgaacttt
    20341 tatgctcttt atgtgtataa ccaagctttt tcttttaatt ctatggggta tgctagtgct
    20401 atttcgtgga tcatgctgtt gattatggga gctgtcacct ttctgatttt caaatcatca
    20461 aaaaaatggg tcgttgtgtg aatgagagga ggaagaaatg aataatacag taacgaaatg
    20521 gtttcaatac ggttttattt tgctattagg tctagtcatg atttatccgg tcttatggat
    20581 gatcgcaggt tcttttaaaa cggatcaaga gattatgagt ggttcgctga atttgattcc
    20641 agagactttt caatgggaaa attacgcaaa aggatgggcg gggtttgctg gaatttcatt
    20701 ttttactttc tttaggaatt cgtttgtcat ctctgcagtt tcgacgatag ggacagttct
    20761 gagctcgact tgtattgctt acgcattatc gagaatcgat ttcaaaggaa aaagattctg
    20821 gttcgtcgtc atgttagtaa cgatgatgat tcctgcacaa gtgattttga ttccgcagtt
    20881 tattatttac aatcggctga atttggttgg gacgtatgtg ccgctgattt taccacattt
    20941 ttttggacaa gcatttttca tttaccagat catgcaattc atggtcagca tccccaaaga
    21001 gttagatgaa tcagcgatta ttgacggatg cagcaagtat agtgtcttta ccaaaattat
    21061 ttttcctttg ctgaaacctt cgatcatcac gacgatcatc atccagtttt attggaaatg
    21121 ggacgacttc atgggaccat tgatttattt aaacaagccg agatcttaca cggtatcgat
    21181 tgccatcaaa ttatttgcag acgctggatc tagtacgact tacgcgagta tgtttgcgat
    21241 gtctacgttg tcgctgctac cggtttttct gattttctta ttcttcaata aatatcttgt
    21301 ccaggggatc agtacaagcg gtctgaaggg ctgaatatga aaaagccaat agaagagatg
    21361 tttctttaca agctcaacta ctatgtctgt cggatttttt taaccagtct tatcagtaat
    21421 gctcttttcg ctttagttct aggatgtttg ttcactttag gattagtgct gtctgctcgt
    21481 gttgtctatc tgatttattt tttgatgatg tatccttctt attatctatc tgtccattac
    21541 ttggcagaaa aaaaggtatg ttggaggaaa ggaaaaatca aacggatgtg tttcatcatt
    21601 acgttgtttc agctgtttgt cattattttt gcagttgatt ggtatttatt agtgggaaag
    21661 attggagctt ggtatctgat tccttttcta ttgtgtctgt tgctttttgt atcccatagt
    21721 ttttttagta cgctaattga tatctgcaaa gaagataaag cagatagtct tgtttatttg
    21781 atcaaaagga atatctggct gttgtcaaaa aacgtcaagg ggtttctgtg gtatttatgc
    21841 cttttgtatg gactgttctg gtgtatacag catgattttt tactcactgc attactgtat
    21901 ccagggctgc catttgtcac agtattttgc ttaaaagaaa aggagaatga ctttaaatga
    21961 agaaaaaata tgtacaagtc ggacttggtg ggagagcgcg ttttttttat gaggcaattg
    22021 cctctacgta tcatcaaact tgtgaactag tcggtttttg tgatgtatct caaacaagga
    22081 tggattatgc aaatcggata cttgtgaaca agtatggaca gaaaaaagta aagacatgta
    22141 tgccatcaca atttgagcaa ctactggatg acgtacggcc ggctgaagtc attattacgt
    22201 cagtagatcg aacgcatcat gattatatca ttcgtgcaat gaaaaaagga tatgatgtta
    22261 tttgcgaaaa acctctgact atcgatgagg aaaaagctca agaaataata acggtccaaa
    22321 aacagacggg aagaagcctg cgagtcactt ttaattatcg ttatgcaccg tatcacacga
    22381 agataaagga actgatagaa agtgatgtga ttggtgatgt gttttctatc cacttcgaat
    22441 ggctgttaaa tacgcaacac ggggcagatt atttcagaag atggcatcga aataagcaca
    22501 atagcggcgg gcttcttgtt cataaatcta cccatcattt tgatctagtg aatttttggt
    22561 tgaacagccg tccccaaaag gtatttgcat tcggtggatt aaacttttat gggatgcata
    22621 atgcggagaa acgcggagta gaaatatttt acagtcgatg ctatggaagc gaactagcta
    22681 aagctgatcc atttgctata gacctgtcag ccaatgagga attgcgagaa atgtatttag
    22741 aagcagaaaa agacagtgga tatattcgtg accgcagtgt attttccgat gatatctcta
    22801 ttgaagatac actaggcgta acggtcaaat atgaaactgg tgcgattttg acgtattcgc
    22861 taaacgcgta tatgccgtgg gaaggattca atgtttcttt caatggcagc aaaggacgga
    22921 tcgaaatgaa agtgatcgaa aagtcttaca tcaatgcagg tggagataaa aaggatgaag
    22981 gagcagccgt tcttcagtcc atccatgtct atccgatgtt cggtgagcca tatgaggtaa
    23041 tcgtcgaaca agaagcgggc ggacatggcg gaggagatcc gcgtttatta gatgatctat
    23101 ttggtgaaga aaaagaagaa gatccgtgga atcgggcagc aactcacgta gatggtattt
    23161 tgtcgatttt gacaggaatc gcagcaaatc attctattgc ttcaggaaaa gcagtagtga
    23221 ttgacgagct tgtcagctta aaagaataag aatagattta cggaaattac ttcccgtagt
    23281 ttataaaatc ttgccttatt gccgataggc tgtcttttga acactattta ttcggaaaaa
    23341 ggggctatga catgcttttt gtcatgcctt ctttttatgt gggctgcttt tttagatagg
    23401 agaaaataaa aataactatt taaacccgaa caaaaaggaa taatgcatta aaaatcatta
    23461 gattttacaa aaaaataagt aaaccacgtt gtcagtttca gaaagtagtg ttagactgtt
    23521 ttcaatattt gaaacaacat tttttattca aatatggcaa actgccttca cgtgcaaaac
    23581 agttgcaaga aaatgaattt acactattat tagttttaca tacctactta tatatcgtca
    23641 taatatggat gacagtttct agccagtacc gtaaatgctg gactataagt aaaagattgg
    23701 ctatttcacc cttcttttct tatgataaag tggagggtgt ttttctttaa cagaaacata
    23761 gtaggttttt gcacaaaagc ggttatttcg gtattaataa atgataaagg agcaaataaa
    23821 gtgaaagaat tagttgaacg tattcaaaaa gacggacgta tattaggcga gggcgtatta
    23881 aaagtcgaca gcttcgtcac tcaccaagta gaccctgtat tgatggaaca aatgggaaaa
    23941 cggtttgcag aagtatttaa agaacaagga atcacgaaag ttgtgaccat tgaagcttct
    24001 gggatcgcac cagctttatt tgctgcaaga gaattagatg ttccaatgat cttcgctaga
    24061 aaagcaaaaa gtttgacgat ggatgaagaa ttattgacag cttctgttta ttcttttacg
    24121 aaacaagtga caagtacgat ttctatttct cgtaaatttt tatcttcaga agacaaagta
    24181 ttgattatcg acgatttctt ggcaaacgga caagcggcaa aaggattgat cgaattatgc
    24241 cagcaagctg gagcgcaagt agaaggaatc gggatcgtca tcgaaaagtc tttccaagat
    24301 ggacgtcagt tacttgaaga aatgggattg agagtcgttt ctcttgcgcg tattgcttcc
    24361 ttgacgaatg gacaagttga gtttcttgag gaggacgcat aatcgtggaa aaagaaacac
    24421 aaaatggaaa agctgcggtt ttagggctgc agcatctgct agcgatgtat gcaggagcag
    24481 tagcggtgcc tcttttgatc ggtacaggac taggctttaa tcaagaacag atgacttatt
    24541 tgatttcaat tgatatcttt atgtgtggat tagcgacatt attgcaattg acagttaacc
    24601 gatttttcgg aattggcttg cctgtcgttt taggatgtgc gatccaagca gtagctccac
    24661 tgatcttgat cggaaccaat gaaggagtag gagctatcta cggatctatt atcgcttctg
    24721 ggatctttgt tgtgttagtt tctggtgtct tttctaaaat taaaaaacta ttccctccta
    24781 tagtaacagg aacggttatc accgttattg gtttgacttt gattcctgta gcaatcgaga
    24841 aaatgggtgg aggtaatgca acagcagctg attttggtga caaaacaaat ctgctacttg
    24901 catttgtgac gattctccta attgtaggcg tgcaaatgct ggcaaaaggg ttcattcgtt
    24961 caatcgcggt attgattggt ttagtcggag gaagcatctt agctgctttc atgggaatga
    25021 ttgatctgag cgcgattgga gatgcgccgt tgttccatgt tccacaaccc ttttattttg
    25081 gtaaaccaac tttcgacgta tggtcgattt tactaatgat catcatttcc atggtcagca
    25141 tggtagaatc aacaggtgta tattttgcac ttggtgacat tactgggaaa aatgttggag
    25201 aagaagaatt gaaaaaggga tatcgtgcag aaggacttgc cgttatctta ggcggtattt
    25261 tcaatacatt cccttatact ggcttttctc aaaatgtcgg attggttcaa ctttcgggaa
    25321 tcaaaacaag aaaacccatc tatttctctg cattcttctt gattgtttta ggattattcc
    25381 ctaaaatcgg tgcgttagca caaatcatcc ctgagccggt acttggtgga ggaatgttag
    25441 tcatgttcgg gatggtggca gtccaaggaa tccgtatgct gttggaagtt gatttcagca
    25501 atgataaaaa tctattgatt gttgctgttt ctgttggatt tgggctgggt ttcaatgtta
    25561 cgccgacatt attcagtcaa atgccgcaaa cattgcaaat gttcactgga aatggcatcg
    25621 taatgagtag cattacggct atcatattga atttgatctt taatggattg aaaaaagatg
    25681 aagaaatatt ggaagaagcc ttcaagaagt aatagagcaa aagaaaggtt gtgaaatcag
    25741 cgttttgacc tgattgttag agaaacaaac ggaaaaatag ttcctcgtat tttgccgttt
    25801 gtttgagcta ataccgaggg tcagctgatt gaacaccgtt gaaggaagag gtcgtgagcc
    25861 tgccgttcat ctccgagtca caaggaacaa tttcaaaaaa aagcttctca tgtttgttga
    25921 atattgttcc ttgtgaccga ggagttggca ggagaacacc gtttatccat aaaaaggaat
    25981 gtgacctaac ttttgtcaca ttcctttttt tttatcttta ataaacacga acaataaaat
    26041 gtttttcttt gttaacattc gaaatttctt tgacatcaaa aaaagtgatt gttaaaatat
    26101 gaatgtagaa aaactgggag gaaaaacaat gacagcaaaa atctctgtga ttatggggag
    26161 tacttcagat tgggaaacaa tgaaagaagc atgtcaaata ttggatgaat tcggtgtgtc
    26221 ttatgaaaaa aaggtcgttt ctgctcatcg aaccccagat ttgatgtttg aatatgcaca
    26281 aaacgctcga aaaaaaggaa tcaaaatcat tattgcagga gcagggggag ccgcgcatct
    26341 gccgggtatg gtcgcatcaa aaacaacttt gccggttatc ggtgtaccgg tccaatcacg
    26401 aacattaaat ggtttggatt cgcttctttc gattgtccaa atgcctggag gagtacctgt
    26461 agctactaca gcaatcggaa aagcgggagc aattaatgct ggactacttg cgatacagat
    26521 gctttccatg tatgatttag aattggaggc taaattggct gaaagacgtt caatgttggc
    26581 taaaactgtt atagaaagta gtgatcaact tggttaaacc tttgttgcct ggttcaacga
    26641 tcgggatcgt cggtggcggt caattaggtc gtatgatggc gattagtgca aaagaaatgg
    26701 gctttaaagt aggtgtgctg gatcctgtga gcgattgtcc agccgcacaa gttgcagatt
    26761 ggcacattga aagtgtctat gatgacacct ttgcgctgaa agagcttgcc agaagaacag
    26821 atgtcattac ctatgaattc gaaaacgtca gtatagaggc gttaaatgcg attttaccca
    26881 tgtcatttat tcctcaaggg acagacttgc ttgcgattac gcaagaccgc ctgttagaaa
    26941 aatctttttt ggaaacgaat aacatcgtga ttgctcctta tgcaacgatc gtcagtccga
    27001 cggatatcca agatgcgatt gacgggatcg gctatccttg tgttttgaaa accactcgag
    27061 gcggctatga tggcaagggg cagtatgttt tgaaaagtcg ggcggatcta gctcctgcca
    27121 tggacctttt aagagaagga acctgtgttt tagaagcatg gatcccgttt gaaaaagaga
    27181 tttcgatcat ggtggcagga aatggtcaag gagatttcac gacttttccg gtcgtggaaa
    27241 acatccacca caacaatatt cttcatgaaa cgattgctcc agccgcgatt gatcaggatg
    27301 tgatcgagga ggctgagcgt attgcccgtg tgattgcaga agcagtgagc ctttcaggtg
    27361 ttttaggggt agaaatgttt ttaacaaaaa caggcgggct atatgtgaat gaattagctc
    27421 ctcgtcctca caattctgga cattattcta ttgaagcttg ttctatgagt cagttcgatg
    27481 cccatattcg aggtatttgt ggttggccat taggagaaaa agtacgtttg ataagcaatg
    27541 cagtaatggt caatgttttg ggagaacaac tgctggaaag ctatcgcttg attgagaata
    27601 agccagactg gcaattccat ttctatggaa aaaaacaagc aaaaaaaggt cgcaagatgg
    27661 gacatatcac tatcttgaca gaagacatcc agcaaacgct ggaagaaatc aaacaaacga
    27721 atatttggga ttagaggaga aaaaacatgt tagaacgtta tacacgccct gaaatgggga
    27781 atatctggac ggaagagaac aaataccgtg cgtggctcga agtcgagatt ttagcagatg
    27841 aagcttgggc agaacttggt gaaatcccgc aagaagatgt gaaaaaaatt cgtgagcatg
    27901 catcatttga tatcgatcgg atcttggaaa tcgaaaaatt aacacgccat gatgtcgttg
    27961 cgtttacacg agcggtttct gaaacattgg gagaagaaag aaaatgggtc cattatggtc
    28021 taacaagtac ggatgtcgta gatactgctt atggctatct catcaagcaa gcaaatgata
    28081 ttttacggga agacttgaaa cgttttacgg aaatcattgg cgaaaaagca aaagaacaca
    28141 aatatacagt gatgatgggt cggacacacg gtgtccatgc tgaaccaact acttttggat
    28201 tgaaacttgc cacatggtat tcagaaatga aacggaacat cgaacgattt gaacatgcgg
    28261 caaaaggagt agaagcagga aaaatctcag gagcagtggg aacgttcgca aatatcccgc
    28321 catttattga agagtatgta tgtaaacaat tggggatccg tccacaagaa atttctactc
    28381 aagttctccc tcgggatcta catgctgaat atttctcagc gatggcgctt attgctacaa
    28441 gtattgaacg ttttgcaaca gagatacgcg gattgcaaaa atccgaaaca cgtgaagtag
    28501 aagaattttt tgcgaaaggt caaaaaggtt cttctgctat gccgcacaaa aggaatccga
    28561 tcggttcaga aaatatggct ggtctagcca gagtggtgcg cggacacatg gtcactgcct
    28621 atgaaaatgt cacattatgg catgaaagag atatttctca ttcttcagca gaacgaatca
    28681 tcttgcctga taccacgatt ttattgaatt acatgttaaa tcgttttggc aatatcgtta
    28741 aaaatctaac ggtctttcct gaaaacatga agcggaatat gtctgcaaca tttggtttga
    28801 tttatagcca gcgcgttatg ctgaaattga tcgataaggg aatgacaaga gaagaagctt
    28861 atgatcttgt tcagccaaaa actgcgtacg cttgggacaa ccaagtgcag ttccgcccgc
    28921 tgttagaaga agatccaacc atcacatcgg tattatctaa agaagaaata gacgatgcct
    28981 ttgattatca tcatcatttg aaaaacgttg acgtgatatt caaacgagta ggtttggaat
    29041 aaagaagagt gagttgcaaa attcaattaa aatagaatag caaaaataac caaaaaagaa
    29101 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
    29161 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
    29221 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
    29281 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
    29341 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
    29401 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
    29461 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
    29521 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
    29581 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
    29641 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
    29701 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
    29761 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
    29821 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
    29881 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
    29941 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
    30001 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
    30061 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
    30121 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
    30181 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
    30241 tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag
    30301 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
    30361 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
    30421 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
    30481 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
    30541 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
    30601 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
    30661 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
    30721 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
    30781 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
    30841 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
    30901 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
    30961 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
    31021 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
    31081 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
    31141 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
    31201 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
    31261 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
    31321 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
    31381 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
    31441 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
    31501 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
    31561 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
    31621 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
    31681 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
    31741 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
    31801 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
    31861 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
    31921 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
    31981 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
    32041 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
    32101 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
    32161 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
    32221 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
    32281 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
    32341 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
    32401 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
    32461 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
    32521 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
    32581 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
    32641 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
    32701 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
    32761 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
    32821 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
    32881 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
    32941 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
    33001 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
    33061 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
    33121 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
    33181 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
    33241 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
    33301 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
    33361 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
    33421 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
    33481 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
    33541 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
    33601 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
    33661 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
    33721 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
    33781 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
    33841 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
    33901 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
    33961 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
    34021 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
    34081 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
    34141 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
    34201 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
    34261 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
    34321 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
    34381 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
    34441 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
    34501 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
    34561 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
    34621 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
    34681 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
    34741 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
    34801 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
    34861 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
    34921 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
    34981 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
    35041 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
    35101 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
    35161 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
    35221 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
    35281 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
    35341 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
    35401 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
    35461 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
    35521 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
    35581 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
    35641 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
    35701 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
    35761 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
    35821 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
    35881 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
    35941 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
    36001 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
    36061 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcg aagaatcgaa
    36121 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
    36181 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
    36241 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
    36301 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
    36361 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
    36421 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
    36481 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
    36541 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
    36601 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
    36661 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
    36721 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
    36781 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
    36841 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
    36901 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
    36961 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
    37021 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
    37081 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
    37141 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
    37201 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
    37261 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
    37321 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
    37381 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
    37441 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
    37501 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
    37561 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
    37621 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
    37681 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
    37741 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
    37801 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
    37861 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
    37921 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
    37981 agccgactcc agcagaagca aaagcattag tgtttgcttg gagagctgtc aaacatgtga
    38041 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
    38101 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
    38161 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
    38221 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
    38281 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
    38341 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
    38401 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
    38461 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
    38521 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
    38581 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
    38641 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
    38701 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
    38761 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
    38821 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
    38881 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
    38941 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
    39001 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
    39061 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    39121 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    39181 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    39241 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    39301 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    39361 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    39421 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    39481 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    39541 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    39601 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    39661 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    39721 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    39781 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    39841 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    39901 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    39961 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    40021 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    40081 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg
    40141 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    40201 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    40261 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    40321 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    40381 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    40441 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    40501 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    40561 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    40621 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    40681 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    40741 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    40801 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    40861 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    40921 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    40981 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aataagaggg
    41041 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    41101 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    41161 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    41221 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    41281 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    41341 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    41401 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    41461 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    41521 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    41581 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    41641 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    41701 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    41761 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    41821 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    41881 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    41941 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    42001 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    42061 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    42121 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    42181 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    42241 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    42301 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    42361 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    42421 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    42481 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    42541 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    42601 ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac
    42661 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    42721 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    42781 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    42841 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    42901 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    42961 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    43021 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    43081 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    43141 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    43201 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    43261 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    43321 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    43381 gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta ttgatcagct
    43441 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    43501 ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt tcttctagga
    43561 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    43621 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    43681 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    43741 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    43801 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    43861 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    43921 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    43981 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    44041 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    44101 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    44161 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    44221 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    44281 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    44341 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    44401 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    44461 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    44521 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    44581 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    44641 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    44701 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    44761 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    44821 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    44881 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    44941 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    45001 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    45061 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    45121 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    45181 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    45241 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    45301 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    45361 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    45421 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    45481 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    45541 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    45601 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    45661 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    45721 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    45781 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    45841 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    45901 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    45961 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    46021 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    46081 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    46141 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    46201 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    46261 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    46321 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    46381 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    46441 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    46501 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    46561 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    46621 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    46681 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    46741 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    46801 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    46861 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg
    46921 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    46981 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    47041 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    47101 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    47161 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    47221 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    47281 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    47341 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    47401 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    47461 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    47521 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    47581 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    47641 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    47701 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    47761 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    47821 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    47881 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    47941 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    48001 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    48061 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    48121 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    48181 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    48241 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    48301 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    48361 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    48421 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    48481 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    48541 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    48601 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    48661 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    48721 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    48781 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    48841 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    48901 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    48961 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    49021 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    49081 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    49141 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    49201 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    49261 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    49321 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    49381 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    49441 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    49501 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    49561 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    49621 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    49681 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    49741 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    49801 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    49861 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    49921 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    49981 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    50041 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    50101 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    50161 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata
    50221 agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag
    50281 atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag
    50341 tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag
    50401 aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac
    50461 cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc
    50521 ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca
    50581 ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca
    50641 gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc
    50701 ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag
    50761 caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc
    50821 ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag
    50881 aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta
    50941 aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa
    51001 tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt
    51061 ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga
    51121 tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga
    51181 aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac
    51241 gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg
    51301 tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc
    51361 cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg
    51421 gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg
    51481 agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg
    51541 atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt
    51601 ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga
    51661 ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc
    51721 ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca
    51781 gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt
    51841 ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct tggatttaaa
    51901 tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact aagaaaaaac
    51961 cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa caaaactgta
    52021 taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa aatagaaaaa
    52081 atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat gctgttaata
    52141 tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt gtatttctca
    52201 taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt tattcactgt
    52261 ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg acaaatggca
    52321 aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc agatttccca
    52381 gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt acctgattta
    52441 tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt attagaccaa
    52501 cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa
    52561 gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct tcttccaggt
    52621 tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc ttttttatgg
    52681 tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct accaccacaa
    52741 attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt gaagaaacga
    52801 aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca aggtgatttt
    52861 tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta tatccaaaac
    52921 aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa
    52981 aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc gcttgcagta
    53041 tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc agtcctaatc
    53101 gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc aacccgctac
    53161 cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg cttccgtaaa
    53221 tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact
    53281 aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca aaaggtttca
    53341 gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt
    53401 tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa gcaatcacag
    53461 atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg
    53521 ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc ggagaagacc
    53581 gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt
    53641 tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc gaagcaatgg
    53701 acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact agaaatctac
    53761 ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg cattcggata
    53821 atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct tccaaccctt
    53881 atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt gttttcctag
    53941 aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct tacgaagttc
    54001 ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc acatacggag
    54061 caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat atatctgttg
    54121 aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt caatccgtgg
    54181 aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc gttgccgcgc
    54241 aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca atcggacgag
    54301 tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg
    54361 caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa
    54421 acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc gcagaaggtg
    54481 aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat acattactag
    54541 aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga acggttaaaa
    54601 atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta gaaatcgatg
    54661 caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc caaactccag
    54721 ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca agcgaaggag
    54781 tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa atcgtcaaat
    54841 ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa gttcaaaatg
    54901 acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac gtgattgttc
    54961 cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct cctggacaca
    55021 atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat
    55081 caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg gctatgccat
    55141 ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact gcaactggaa
    55201 aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg
    55261 caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct cctgcagcag
    55321 aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac ttggaagaac
    55381 gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac agtaagcata
    55441 cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg gataatcgga
    55501 aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg ccatatgtcg
    55561 tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct atcgacgatg
    55621 caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact gatacggatc
    55681 acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt gctattgcag
    55741 acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca gaagatatgc
    55801 gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg tttacaccag
    55861 tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa caaccaatcg
    55921 taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc
    55981 accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa cgtcttttag
    56041 ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg agattttgct
    56101 gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc tgccatccgc
    56161 gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg cggtgtttgt
    56221 ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca ttatcaagag
    56281 tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa
    56341 acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt tggtttccta
    56401 ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca
    56461 ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc aatcatcgca
    56521 acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt gttagattct
    56581 actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg tggcggtgtg
    56641 attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac gattcttgaa
    56701 ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt agaagatgat
    56761 ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac
    56821 ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt tactgctgat
    56881 tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt agaacaagcg
    56941 ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg tacgaacgta
    57001 cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca caaagccagt
    57061 tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt agattataaa
    57121 gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat gacgatcaaa
    57181 gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc agggaacggt
    57241 cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac gatcgaagat
    57301 aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta
    57361 gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat tcatcctcat
    57421 ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt accaatccat
    57481 atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta
    57541 gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg gtttattacc
    57601 acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg caagaatatc
    57661 agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg aagatgtggg
    57721 aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg aagacttatc
    57781 agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga cgggaatttg
    57841 aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca
    57901 aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata tcaagtcctg
    57961 gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa
    58021 atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt ttctaatcga
    58081 aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag ggtgtaatca
    58141 attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca ccttgaattt
    58201 tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg gcgttggaaa
    58261 gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa aaggaatagg
    58321 cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat cggaaggctt
    58381 acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag aaattgcgga
    58441 aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg caatcttgaa
    58501 aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag cgggaaatgt
    58561 cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt tgacatttga
    58621 aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg cagacctttt
    58681 gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa
    58741 agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa aagtctgcta
    58801 taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa ggagttagtt
    58861 acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga
    58921 aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc acatacgcaa
    58981 ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta
    59041 gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga tactccagga
    59101 cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg
    59161 gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa aaaagcactg
    59221 gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc tgctcgtcct
    59281 gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga tgatgatcag
    59341 ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt gtcagatgat
    59401 ccagcagatc aagaaccaac catggcacct atttttgata caattattga aaaaatccct
    59461 gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact tgactacaac
    59521 gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa agtaggagac
    59581 caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac gaaattattt
    59641 ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga cttgattgct
    59701 gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat
    59761 gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt agttaataat
    59821 tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga agaacgtttg
    59881 atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc tccagatgca
    59941 tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa catgcgccgt
    60001 gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat cgacggtgta
    60061 aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat gggtagtgtt
    60121 atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac aggaaacgga
    60181 caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc aactgaattc
    60241 ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata tctgccaatg
    60301 cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa
    60361 gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt
    60421 acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga tttgactgtc
    60481 aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga ccaaacatct
    60541 gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt gaatgatgat
    60601 gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt agagaaaaat
    60661 gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag agagaaacca
    60721 gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt ggagaagtca
    60781 tctttgtcaa tggacataaa ttcagaaaat acgccgaaag gcgtttaaaa gacttttaaa
    60841 aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga aaggagatca
    60901 acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg
    60961 acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa gaaattatcc
    61021 agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt gggaaatcaa
    61081 tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg tcccctttaa
    61141 tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc gccactttta
    61201 tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc gatagacagc
    61261 agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt atccaagcaa
    61321 tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc tctcagtggg
    61381 ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa ctgccaaatc
    61441 gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct gacataaaaa
    61501 gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga gaaaatctac
    61561 gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat ttaaagatca
    61621 acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac cgtctctatc
    61681 atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta aatgagaatg
    61741 agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt gtagcgacaa
    61801 atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat tatcaaattc
    61861 ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat ggtctttcca
    61921 gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt tttatccagc
    61981 agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa gcgatgacag
    62041 aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt ggcgagacaa
    62101 gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta gaagtaacga
    62161 cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa
    62221 tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta ggttttggac
    62281 atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg
    62341 aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct gttttgaaag
    62401 taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga aaggaaccca
    62461 agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg ctgcgggaac
    62521 tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt tcagattcca
    62581 cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt cagataaaag
    62641 gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg ataaaaaatg
    62701 cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa taaaaacaac
    62761 tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat taaggaatta
    62821 gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa
    62881 tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc tctgtatctc
    62941 acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt cccggcaagt
    63001 cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga tgttaagtga
    63061 gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt atgataaaaa
    63121 agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca tctattagac
    63181 tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat ggtatatagt
    63241 tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt cattaaccag
    63301 agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat gaaaacagat
    63361 gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat tttgctgctg
    63421 attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca aattgcagga
    63481 ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct gtctctgact
    63541 ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa acgtccactt
    63601 gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg taatgcggca
    63661 gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta tgtgtatact
    63721 ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct gatcaatatc
    63781 acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt tgctatttac
    63841 caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg ctactatgcc
    63901 atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa aaaaggattt
    63961 ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact gggagtgatt
    64021 atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt ccttgtaggt
    64081 atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc aatgtttttg
    64141 atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac agggatcacg
    64201 ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg tgtcggcttt
    64261 gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata aaaaaagatg
    64321 taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt
    64381 atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag aagaagatga
    64441 atattccgta caccgtttca aagcggatga agcatattta gtaggacaag gaaaaaaacc
    64501 gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat gcggagctga
    64561 cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga
    64621 agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt ttggagataa
    64681 aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag gcacagatgg
    64741 accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt atccgattat
    64801 gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg atgaaaagag
    64861 tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga
    64921 agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga ttctcgggga
    64981 tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc gccgtcacca
    65041 aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg
    65101 ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt
    65161 tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc aagtggaaca
    65221 tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt tgattgctca
    65281 aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta aattaaatgg
    65341 ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt taccagacac
    65401 agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg atgtcggaaa
    65461 tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa aagtatgtac
    65521 gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga aagaatttag
    65581 aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac atcctgaatt
    65641 ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat ttgaatttcc
    65701 tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg
    65761 ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga
    65821 tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga agggtgccga
    65881 tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata cgacatttag
    65941 agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca agcagattgc
    66001 acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg ggggagcaac
    66061 atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga gaaaaattcg
    66121 ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta
    66181 ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag cgcaaggaat
    66241 cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa aaagtatcca
    66301 agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg gcgatatcaa
    66361 cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa aggaactaga
    66421 acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta aaccgcaggc
    66481 agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc atctgcatac
    66541 tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag caggagtaga
    66601 catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta gcatgagcag
    66661 cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg aaaatgcaca
    66721 gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg aaaacggctt
    66781 gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt attcgaatct
    66841 acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca aacaaatgta
    66901 tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat ctaaagtagt
    66961 tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata tttatgaaaa
    67021 aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag aactagggca
    67081 acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca gacctgcatt
    67141 gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt
    67201 ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt tgatgtatcc
    67261 gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta cattactgga
    67321 cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg
    67381 cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg aagggaatcg
    67441 cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag ataattcgat
    67501 catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac aaattggagc
    67561 aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg
    67621 tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag cacgttttga
    67681 cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg gtgacctttt
    67741 gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag actgggtttg
    67801 ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt cttgtttccg
    67861 ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat gacaacgtgg
    67921 aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc gatcgatcag
    67981 ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg ttttgaaaca
    68041 agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca agacagcaga
    68101 gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg
    68161 atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac ttttgagtac
    68221 catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg tccgctaatt
    68281 gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa cggcctattt
    68341 tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca agtgtttggt
    68401 gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat caatcgcgtt
    68461 aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga acttcccata
    68521 tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga ttttttgaca
    68581 aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc gctaggcagt
    68641 gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt aatcagtaaa
    68701 caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc tactttttcg
    68761 tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc gggaacggat
    68821 tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga agaaaacgga
    68881 gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg atcaatgcct
    68941 ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg aacataaaca
    69001 gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga
    69061 agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg aaaaaaaacg
    69121 agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag ctttccgata
    69181 tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg ccgaagaagt
    69241 ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa aacactttgg
    69301 atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca aaacgacgcg
    69361 ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt
    69421 tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa gagacattgg
    69481 aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa cgttcttacc
    69541 gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta ttaaaagacg
    69601 aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat ctttaaaaat
    69661 ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt ttgatcattg
    69721 tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga gtagatgcgg
    69781 ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga
    69841 ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata ttttgatgga
    69901 gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc agtatctaga
    69961 ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat catcaaagaa
    70021 aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga tgcggacaaa
    70081 gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct tgatccgcca
    70141 tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg acttttttct
    70201 aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga aactgtggct
    70261 ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat ctatagaaaa
    70321 gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt acaaaaggtc
    70381 acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt ggtgttttta
    70441 tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg atcacaaaag
    70501 ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa
    70561 cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc aaagactttg
    70621 aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg gaaactgtct
    70681 tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa gaagtactgc
    70741 actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct ttagctggaa
    70801 agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat catatttgga
    70861 atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc accgggagcc
    70921 acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg ttctgggtct
    70981 ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa
    71041 atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc gacaaatgaa
    71101 gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc aatcgaacaa
    71161 gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata tgtcatgagt
    71221 gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt aacaaaagta
    71281 gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag ccaaaaagtt
    71341 ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc ttctggtgat
    71401 ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac tgaccatacc
    71461 gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg gccatcagcg
    71521 ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt gcgacatggt
    71581 aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg aatcggagga
    71641 atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt tgcaccagat
    71701 gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa
    71761 gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta
    71821 caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa agaggggttg
    71881 tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa
    71941 acaaaagaaa aaattgacgt aattagatgt tgaagggacg attttgccag agctcactgt
    72001 ttcttcaatt ttaaatgaaa ggagtaatga atgattttta ctggtcattc tttgaaaaaa
    72061 gaacaatgat agaaaaaatc agcaagaaaa tactagcttc acccaaattg atcatttgga
    72121 tgtctatgat agttcttatc ttttgtatcg caagtggttt tttattatca aatttgctat
    72181 tttatagaga aacagaagag actgctgcaa aagaaatact ttcttcgttt agttccagtg
    72241 gagaagatcc atcagctgaa ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt
    72301 acaccgatat caaaggaagt gtgaaggagc ctggtatcta ttccttttct tcagaagaac
    72361 gggtctatga tgtactaaag cgtgcaggtg gattattaga agaagcggat agcgatcgaa
    72421 tcaacttttc tgcgaagata gaagatcagc aagtcctata tatccctgct gtaggtgaag
    72481 agctgccaga acatcttgat cagttggcat cctcggaagg aaaacagcct acagcagatt
    72541 ccgagcctcc caaaataaat atcaatacag ccactccatc agaacttcag cagattcctg
    72601 ggatcggatc agtcaaagcg caagaaatca ttcgatttag agaagaaaac ggatccttcc
    72661 agaaagtgga agacctgcag gagatttctg gtatcggtga aaagacagtt gaaaaactaa
    72721 aaaacttcgt tacaattaaa tagaatgatt caaaatcgac tggcacgagc aataaatgga
    72781 cgctaatgga tactaagaat aatccaattc taagagcttg gcaggacgag gaggtagtat
    72841 ttttggatag gaaatagctt gattgttcgc aaagaaacat taaaaaaacc aatgctgaat
    72901 gtctatctcc aaaataaaat atctgggatt catattatga atacggctgt cagcggcaat
    72961 aacagtcaag ctctacgaga acgctttgcc aaagatgtac ttagctatac agcagataaa
    73021 gtatttattc tcattgggac gaatgatcta gcagagcata aacaactttc taaagaaacg
    73081 taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa agactatagt tctgatcatg
    73141 ctttctttat cacgccacca gcagtcgatg agacaaaaca aaaatatcgg aataacaagt
    73201 tgatcaaaga atatatctct gttctcacag aagtagcaaa acgccatgat tgtgaagtat
    73261 cgatttttat acggaatttc ttgaacaaat ggattcgaag aatttttata atgggctaat
    73321 ggatgacgga cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg
    73381 ttatatttaa agaatatttt gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg
    73441 gaggaatata aaacatgtta gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag
    73501 ttactgataa gtcacataat gaacgcattc cttgggacca gtactttatg gctcaggcag
    73561 tactgttatc tttacgtagt acgtgtacac gtttagaagt aggagcgaca ctcgttcgag
    73621 aaaaaaggat cattgctggc ggctataatg gagcagtcag tggagatgtc cactgcatag
    73681 atgaagggtg ttacgtcgtg gatggacact gcttgcgaac gatccatgca gagatgaatg
    73741 cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc tatgtcacac
    73801 attttccatg tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt
    73861 atctccatga ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat
    73921 ctgtacacca agttgcttta gactcacagc atttttcaaa acttcaaaat gaattaacag
    73981 gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt tccggcaatt
    74041 acgtcgatga ttatctgttt gttgtattac actgacaatt ggggttatcg tttgcttctt
    74101 gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt
    74161 ttgtgtagca taacggtatc catttcttat aggctacaag agtattcatt tttagaggaa
    74221 aaaacaacat ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa tggtaatttt
    74281 ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc tccagctttc ttatcaagta
    74341 aacacagaag cagaattgaa aacatggctt aacttccgag gaaagggaat agaattgact
    74401 gctgaaggcg agttcatctc tccagcaaaa aagcggaatc tttataattt tgaccaagaa
    74461 gcttacttca ggaaccatag aatttctgga cggttccaaa ttcaaaagat tgaacagatc
    74521 accatgcaaa atcactggcg aaactggctt cagagaaaaa gaggatcttt tatctccttt
    74581 gtccaatctg aatttccgca aaaaacaagt gtatatatta attctttatt atggggctat
    74641 aaagatgcag gattcagaga accagaagaa atttttaggg agagtggttt gctgcatctg
    74701 ttcagtctat cgggtctaca catccaattt tatttagggt ggctgtatta tttatttcga
    74761 cgtatcggat ggccactaca cgtcagtatt attccgttga gtctactaac tatctgttat
    74821 gtttctattg caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga
    74881 ttgctagtca aattgctgaa taagcattat tccaccatgg acttatttgc agttgtcttt
    74941 tggcttcttt tactatttga tcctcgattt ctttttcatg cgggtggaca gcttagtttt
    75001 ttcatgagtt ttttgtttat cctgatttct tttgatggag attggttgaa aaaaacggcc
    75061 tctcacgtcc tgtttactat gatcacgatg ccgttgattg tatggcattt ttatgagtgg
    75121 cctattttag gtagtctttt tacattactg attgcaccgg tattcaagtt tctttttctt
    75181 cctggtgtgt tatttctcac actcttcaat cgctggctgc cggaaacttt acttttgttg
    75241 atagaagagg gattaatctt atttgagcga ataatcgatt tttcttcagg gtcgtctctc
    75301 attataggac gtttgcccct tgtgctatct gggatgtata ttttcggatt actgatttgt
    75361 atggataggt tgaaagaaca acctgtgaaa cacttgtttt atgttggtct tttttctttt
    75421 atttttttga ctttgccatt tttacgcttt tcttcaacga tcgcttttat cgatgtcggt
    75481 caaggagaca gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt gatcgatact
    75541 ggaggtcagt tacactttga aagagaaaag tgggcgcaag gtatgatacg tcctcctgct
    75601 gaaagaaata tcgttccata tctaaaaggt caggggataa gtgtaatcga taaactcatc
    75661 ttgacacacg acgatacgga tcatgtggga gaattaccca cgttatctca gcattttact
    75721 atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg agccattaca aaggcattta
    75781 tctgaagctc aaaaaaatgg tactgagatc attgaaatca aacatgggga caggatcagc
    75841 ggttattatg atctttatgt attgactcct tttgaaaagg gagagggaag aaatgaagat
    75901 tcgattggtc tatggatgga atataacaat aggcgctttt tatttttagg agatttgaat
    75961 caagcaatgg aaaaacaatt gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa
    76021 ttaggtcatc acggtagccg tacgtcaagt aacacagatt ttctttccca tattgaagca
    76081 aaacatggga ttatatcctg cggcgtaaat aatcgttatg gtcacccgca cagcgaagta
    76141 ttggagacac tagaagaaaa tcagataaaa acatggcgaa cggatcaaca aggaatgatc
    76201 acctatcgct ggcatccagt ttttcatcct catgggctag tggaaacaat gatagactag
    76261 taattctcat ctgctcatgg taggataatt tttgaaagtg gtgagtaatt tgagtataca
    76321 agcagagtta caaaaaatac gtaaagaaaa acttgcatct tgttacttga ttttaggaac
    76381 agaaaagttt ctccaagatc aagttcggac agaaattgtc aaaaaaatcc aattagaggg
    76441 cgaagatgac ttaaattttt taagttttga catggaaaat gcaagtttag atgaggtagt
    76501 agctgaagca gagacgttgc cgtttttcgg agatcaacgg ttagtattcg tagagaatcc
    76561 ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg atttattgac
    76621 ggcttatttg aaagaaccat tagaatcgac tgtattagta ttctttgcgc catatgaaaa
    76681 gttggatgaa cggaaaaaag taacgaaaca attgaaaaaa acagcaatta tcatcaatgt
    76741 ccaacagttg aatgaaaaag aagtacgtca atatctgatg aataccttag agaattcctc
    76801 aatcaaaatg gaacgaagtg caattgattt attcttgagg ttgaccgact tagatttatc
    76861 caaaatgatg agagagttac agaaattggt cctttatgga caaaatcaac agcaaatcac
    76921 ggtaagagaa gtagaagaat tagttcctaa aacgttagaa cataatattt ttgacatgac
    76981 ccaatatatt ttaaaaggga agacggaaca agctttgcgt ttatacgaag atttagttat
    77041 gcaaggggaa gaaacaatca aaattaatgc gattttgctt tctcaattac gtttattttt
    77101 acagacaaaa tttcttgtaa agattggtta tcagcaggca aatattgctg aaacattgaa
    77161 aatccatcca tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg atgaacattt
    77221 gctggtacag ttgtttgatc aattagttga gatggattat caaatcaaaa cgggacaaat
    77281 cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca gggcagttgt taagaaatcg
    77341 ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg
    77401 acaaataaaa aaatcggccg aagccgattc ttttatttag cgattttttt gcttaaacga
    77461 gatttgtcgc ggttagcttt gtttttgtgg attagacctt ttgattcagc catatcaata
    77521 gctttaacag cttctttata taacgcatct acgttgtcag cacctgaagc tacagcatct
    77581 tcaaattttt tgattgcagt acgcatagcg cttgtttgag atgaattttt cacattagcg
    77641 ttttcgcttg tacgaacacg tttgattgca gattcaatgt ttggcattag tttcacctcc
    77701 gataacttaa gttcttacgc gtgttaccgc ataattcaac attcattatt atacctaaag
    77761 gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc ttaacagtct tttattagga
    77821 ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg
    77881 gaggcaatag cgtcaaacca aactagattt ttatttatct atttaaacaa agtttgaatg
    77941 ggaatgtttg gtactaagtt attatataac aaagagaacc gtttgcaaag ttttttttga
    78001 aaagggatac gattccctgt tgttctttgt ttttctatta agaaactgca gttattttat
    78061 aaccagtatc ctttgatcag taaaggctat tctggtgtac cgattatttt aatgtattca
    78121 tcgatatttt ttatcaacat tttttttatg accaactggt tccgacggtt ccatttgcca
    78181 cttacttcca gcacatattt atttaggggc aaacgtaaga tatattttgc caaatagcga
    78241 tctttaagca ggccattgat cacacggtcc tcaagctgca agttgatccc agtcagcatc
    78301 attggctgtt cttgtaaaac gtacgtatca attaaaagac cttcaaaagt ttccacaagc
    78361 gtcttccttt cttttcaaag taacctgaaa acaggcacta attcttccat tttgatattc
    78421 gttgaagtgg atgcgatcat tctttctttt gtccactgta atctagtata acaaaaaaga
    78481 gcagaagagc caaaaagaaa aaagagcatc gaattctata ggaagtctca ttggcacggc
    78541 ctaaattatt acaaaacatt gattaaaaaa agcccctgca tcgcaggagc atcccctata
    78601 tcgttttaat aggtcaatca aaaagcagta tcaaacacat tttgtatcaa atcaggaaat
    78661 aatagataag ctaagcccag aatcaaaagt accgctagaa gaaaccagcc gattgttcct
    78721 atgaaatact ttaatttttc gaacattgta tcacctcgtt gtcagtatat cacggatcag
    78781 taaattgggg gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc
    78841 aaatacttgt aagatacagt aagtgatacc agaagcaatg accggtccgg cggcgattcc
    78901 tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc
    78961 agaagcaatc aatcctacac ccttggaaga taagacagcc actagcccac cgcaaaaaac
    79021 agctacccag ccaatagggg atttaaaggc ttctaataga tcacgtagac cgatttctcc
    79081 tgttgcgata gggattagaa tagccgctgt gatgatcgtc accccaatat tgattccttt
    79141 gccttgaagc caagggaaaa aggaacggga ggctggtatg agcttcataa tcaaaacaaa
    79201 agctgccgct atcatcagac tttgattttt agctaaaatt gctacaagta aaataattcc
    79261 taaaaaaagc caactttcca tgaaatatat tcctcacttt cttcgaaaca atcataacat
    79321 gtttttagaa agttacaaaa gtaggaattc atccttcaag acctattcat aagctgtttt
    79381 tttcataaaa taaacaaaat cataaaaaaa cactttgctt atttgacaga agacagccgc
    79441 gctttgtata gtggacaaga cttacatttt tttgaagaat cagaaagatc aggtaaaggt
    79501 cttagtaaac gcaagccatt cagaataacc aatattgtgc ttccttcgtg tcctacaact
    79561 ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag aataacaaga
    79621 atcgaaaaaa taatattttg tttgatgatc cgccgcattt ttaaagatag ccgatgactg
    79681 tagacaaatt tatttaagtc gcttttcatt aagacgatgt ctgcgatatc catagcggca
    79741 tctgtccctt gccccatagc gacacctaaa gaagcggctg ctaaagcagg cgcatcattg
    79801 actccatcac caaccatcgc atttacttgg taaagttcat tctctttttt gactagttgt
    79861 gttttttctt ccggagtaca gcttgcatag taatgttcaa tccctacttg ttcagcaatg
    79921 gcctttgctg tgccagaatg gtcgccggtt atcatacttg tatggatgtt ttgggattta
    79981 aagtaggaaa ttacttgttg ggtatttgct tttggaatat ctagcagacc taaaacagca
    80041 attaactgct gatctttaga aagataaatc acggttttgc cttgattttc caaacgttcg
    80101 atcatttccg caatctcttt gctgattatc atcgtctcag gatcatatgc gtgcttgcca
    80161 actttccatt gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat ttcttctatt
    80221 ggtaagtatt ggatttcttc aggtattgtg caatcgaaat attgcaaaat cgcatgagca
    80281 agtggatgcg tcgttttttg ttccatagca acaagaagtt gttgtgccaa atccttttca
    80341 tcaaagaaaa agctgtcagt tacaactggt tttcctttag tgagcgttcc tgttttatca
    80401 aaggcaatcg cttttaattc agaaagcttt tctaaatgga taccgccttt gattaaaatc
    80461 ccattttttg cagaatttga aagagcagct agagaagctg gtgtagcaga tgcgacaagc
    80521 gcacaaggag aagccacaac taataatacc attcctcgat aaaagctttc atcccaactc
    80581 caacctagga aaaagtgggg gagaaggatc attagaggaa cagcaagcaa aatcaatttt
    80641 acataattat tttcgattcg ttcgataaat gaagctgttt gagttggcgt attctgcgct
    80701 tcttctacta attggataat cttagcaaag acagtttcgg aactcgtttt tgttactcga
    80761 atcgtgatgg gatttccttg attcagcgtt cctgcataca tctcatcgcc taaatatttt
    80821 tctacaggca cagattcacc gcttaatgca gactcatcga tcgttgcctg tgtgctttct
    80881 aaaaagccat ctatcggaac agctgctcct ttagcgacaa ataccaagtt atccaccttt
    80941 agttgagaaa catctacttc ttgcatttgt ccattttctt ttaataattg ggctttttgt
    81001 ggttgaagat tcatcaaagc agtaatttct ttttgacttt tattagttgt gtattcctct
    81061 aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta attaccgatc
    81121 aaacaggcac caatagcagc taaagccatc aataaatcca cattcagtgt ccgatcattc
    81181 cacaattctt tcaagccttc cgatgtttgt ttatatccac cgatgataat ggcactgata
    81241 taaaaaagtg gggtgatggt aagagagaat cggtcgaaga taaaaccgaa gatcataaat
    81301 aaaaggcagc cgattgttgc tgcaaaagct tttttctgat tagtcatcgt tattagacct
    81361 ccttgtcaaa acaaggatag cataataaaa atgtaattga taataataac agctaaatga
    81421 gaacgataat ggttatcatt tggtgattaa gcttgatgtt gtttttatta tttataatga
    81481 ttattattaa agaaaataaa aaaccagaac atttctcaaa taagagaagt gttctggttt
    81541 tttctacatt gcaataaacc aataaccaat caaaatgata ccaaggataa tacgatacca
    81601 gccaaaagcg gtaaagtcgt ttttcttcaa atagcttagt aagaatttga tagctaagac
    81661 ggaaaccaca aaagatacga atgttcctac aagaagaaca acagtttctt ccattttgaa
    81721 tgaattccct tcagctaaat atttgacgat cttcaagccg cttgcgccaa acatgacagg
    81781 aatccctaag aaaaatgaaa actctgtagc gacaaaacgc gaagcaccaa tcaagataga
    81841 tcctaggatc gttgcgccag aacgggatgt tccgggaaca agtgccaaga cttggaataa
    81901 cccaacaaga agagctgctt ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt
    81961 gtttttgttg cgtttttcga caataataaa ggcaataccg taaacgatca aaacaatggc
    82021 aactggtaag aacttgtgga aatttgcttc taaccaatca tccaaaggca aaccaatgat
    82081 gatggctggt atgcaagcaa cgatcacttt aaaccaaagt gtccacgtat cttttttttc
    82141 taccgttgat ttacttggag aaaatgggtt taacttgtga aaataaagaa cgacgacagc
    82201 caagatcgcc cctaattgga taacgacatt gaacatcgac ataaattgtg aactggcatc
    82261 cagtttgatg aattcatctg ctaggatcaa atgaccagta ctactgatgg gcagccattc
    82321 cgttattcct tcaataatac cgagaatcac tgctttgata atatttgcaa aaaacataaa
    82381 ttccttcctt cccaattaag atacatctta tagtataccg ttattttttg agaaacccaa
    82441 tctgtttttt aatattaaga aaaacttgag aaataaagca gataagtaca cgaagagaaa
    82501 aaagaagaca aagccctcgt tcagactttg ccttttatga ttctctggta aaaattaaaa
    82561 tatccccatt atagaaacga gtcgcgacta tgtgttttcc cgttatcgat ataagacgta
    82621 tcattgatgc cgtggataga aaatcgaccg tttgcataag tgacctttgt tacgctgcca
    82681 tttaaaagcc cagcacgaac aggctgttta ggatcgatca gattaagcaa aaaggcgatc
    82741 gtcaaaccat gagatacgac taaaacattt ccaccaccat ttttttcgca gtgatgggcg
    82801 atatcttcaa atcctgtcca tacacggtct cgcaatgttt cataagtttc cgcccaccca
    82861 gcggtatcta agttgtatac agcattagca atttgttcaa aagaaacttc tgtggtaaac
    82921 atttcatcat atgttttaaa attcaataca cgagggatca caccccacat ttccatgtca
    82981 tatccacctt caagggaccc gaaacaccat tctctaatcc ttttttcaat aaaataagga
    83041 atttcttttc ccttagaatg ttctcctaga ataatacggg cagtttcgat tgcacgacca
    83101 ctgtcactag aataagcaaa tgaaaagtcg acatttttta acccgcgacc taaataatga
    83161 atgccttctt gtccttgctt tgtgaggggt gtatcacacc agccttgtac tcgttcaatt
    83221 gtattgaaca tcgtttttcc gtgtcgtacg atataaagtg tagtttctgt cattttattc
    83281 tcctcctaag tcgttttaac gattgaaaaa aggatttgtc cttttttcct gttcaatcgt
    83341 cgttgcatcg ccatgtcctg gatagactgc aaattcatca ggtaatacaa acagctgggt
    83401 ccgaatacta tgaagtaatt gttctaaatt ccctgtatac agatcggttc taccgatact
    83461 tcctctaaac agggcgtcgc cagaaacgac aaaatcatca aaaataaagc tgacgcttcc
    83521 tatagaatgc cctggggtag ggacgacgcg aaaagacatg tttcctaaag tataagtttt
    83581 catttcaaat tcatattctg caggcgagac aataacatca gcaatatcat catgtctacc
    83641 gagtccagaa agattcaaaa tcggatcacc tagccatttt tgctctagtg ggctgacata
    83701 aactgggatt tgataatacg cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt
    83761 atgggtaagc aaaatcgcta ccggctgttg gtttgttttt ttgatttctt gtttgattcg
    83821 ttccgcatct tctccagggt caatgatcaa aagtgcttct tcgttgtaga ccaaataaca
    83881 gttttcttca atcattcctg tttttaatct ttcgatatgc atgtgcattc ctccttgata
    83941 gccaaaactt ctttcatcca ttagtataac gcattcattg ctaggaaaca aaaacaggta
    84001 cttatttctc tttacttatc tgaattatca gactatatga aaagtatttg gatattctaa
    84061 agagaaggaa agtaagtttt catagcattt taatatgtcc ttgttacatt agattcatcc
    84121 aaaaatccaa attctatatc ctatatccct gcctaatcag cagggtgttt ttttatgttt
    84181 ggaaagagag ctctttttgc taaactaggt tttttcaggt aatcttagag agaaagaggt
    84241 ggtggaaatg atcaaacagt caaaaatatc tgtgcggcac ttagtagaat ttattttgcg
    84301 acgtggaagt atcgacaatc gtaaaaaaag taaccatact gcattagaag gagcgagaat
    84361 ccaccgcaag ctccaaaaag atgcgggaga aaactatgaa aaagaagtct ttttaaaaac
    84421 aacagttcaa ttagatgaat attacctgat cgttgaaggc cgtgcagacg gtattttcaa
    84481 aagagacggt atttattata tagatgaaat caaaacctcc gaaccacgat ttgaagattt
    84541 agaaccagaa caagtggaac ttttctttca tcaagcaagg gtgtatgcct atatctatag
    84601 tcaagaacat gatttacaag aaataaatct gcaattgact tacttccaga cggcagaaga
    84661 agtgatcacg agaaaggttg aacatcagac aagagagcag ttagaaactt tttttaaaaa
    84721 gctgactgaa gattataaag aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa
    84781 tgcctctttg atggctttga aattccccca tgatgagtat aggaaaggtc agcgtgaact
    84841 agcggtagct gcttataaaa cgatccgtac gaaacagaaa ttgtttgtag aagcacctac
    84901 cggaacagga aaaacaatct ctacgttatt ccccgctttg aaagctgtag gggaagaaga
    84961 gggagaaaag atcttttatc taacggctaa aacaataact agacaagtag ctgaagatgc
    85021 tatgacagca ttaaaagata caggagctga agtaaaaagt gtcactttga cggcgaaaga
    85081 caagatctgc tttttagatg agacgacctg taatccagat cagtgcccat atgccaatgg
    85141 ttattacaat cggattaatg aaggtttatg ggatcttttg aatcatgaga atcagatcac
    85201 tcgtgaagtc atcgaaactt atgcaagaaa gcatacactt tgtcctttcg aactatcatt
    85261 agatgtcagt atctggtgtg acgtaatcat tggggattac aattatttat ttgatccaac
    85321 tgtttattta agacgttttt ttgaagatga aaaaaacgag gactatctgt ttttgattga
    85381 cgaagcacat aatttggtga atcgttcccg agagatgtat tcggcagaac tatcttatga
    85441 aaaaacgaaa cgaagtaagg aagctatacc aaaggaattc aaaaaacttc atcgccgttt
    85501 caacaaacta ttaaaagaat ttgattcgat ccgagaaata gcaaaagaag atcattggga
    85561 ttatcatcat caaaaagcac cggctgaatc tttagtaaag gcaggttatc aattaagcga
    85621 aaaaattaaa gaatggctgg cagaatttcc tgagcaccca tcacaagaac aattgcttcc
    85681 ttattatttt gatttgcttc attttttgaa ggtcagtgaa ttttatgatg atcactacga
    85741 aacaacagtc gaaaaaacgt atcgcgatct gatagtaaaa gagttttgta ttgatccaag
    85801 cttgtttctt gagcagtcgt tggataaagg aaaaagcagt attctttttt ctgctagttt
    85861 ttcaccactc tcttattatc aggaaacact ggggggacaa aaaagtctgg cttacaaact
    85921 gccgagtcct ttcccagaag gaaaccagca agttttcatc gcaaattacc tagagacgac
    85981 ttatcgaaaa agggaacaca gtctggcaaa attagtagag acgattcatg cttttgctga
    86041 tggaaaggtc gggaattatt tcgttttctt tccttcttat caatatttag atcttgcggt
    86101 ggaagcattc agacagcaat atcccgacag caatgtgcta atccaagaaa cggacatgaa
    86161 tgaggaagaa cgcgaacgat ttttaacaaa atttcaaaca aatcccgctg aaacattgat
    86221 tggattttgc gtacttggcg ggattttttc agaaggaatc gatttgagag gggaccgatt
    86281 gatcggcagt atgatcgtag gtgtaggttt gccgcagatg aatcatgagc aggaactgat
    86341 caaatcatat tatgatgaga aggaacacct tggatttgct tacgcttatc agcttcctgg
    86401 aatgaacaaa gtcttgcaag ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat
    86461 cgttgtattg gcagatcaac gattttctag tttttcttat cgacgtttat tcccacaaca
    86521 ttggcaaaca gcaagagaag tacgaagtgt tggggaattg gaagaaggaa tacagcgttt
    86581 ttggttatca aaagaagcag taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt
    86641 ttttgattaa cgatagttag tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa
    86701 agcaatcaca gcttgtagat catcacggtt cttacctgtc actctaatct gatcttcttg
    86761 gatttgtgct tttactttca gtttttcatt tttgatagct gtagtgattt tcttcgcgtt
    86821 ttcacgatcg attccgttga ccaaatcggc agtttgtcga gctttgccac ctagagcgtg
    86881 ttctgattcg gaaaaatgga tatttttgat tggcacattt cttttgatca gcttaccaaa
    86941 taatacatct ttgatctgtt cgattttgaa atcatcatcg ctcaatacaa ctagattgct
    87001 gttctctaat ttgatttcta ctgtagaatc cttgaagtca aagcgattgg acaattcttt
    87061 tgttgtttgc tgaatcgcat tttttacttc ttctgtattg acttctgata caatgtcgaa
    87121 gctagcatct tttgccatag gaaccctcct gatatacata taaatattta ccatacatct
    87181 atcttatcat aggattaggg cagattctta cctgtttgct aaaattcttc tcatttgagt
    87241 tcgaagtatt ttattttaat agataaactg ttattcttac atttggaata ccaaagtagg
    87301 ggagaggtgc ttgtgagtaa aattatgact gcgtatcata aaaatgaaag attaaagaaa
    87361 atagtggtta ttttgataac cggattgacc gctgcggtcg ctttaaatta ttttttgatt
    87421 ccagccaatg tcttttctgc agggatgaat gggatcgctc aaatcatttc ctcgctttta
    87481 tatgaatggt ttcatatcaa gatagacaca ggaacattta tttttctttt gaatatccct
    87541 gtgtttgtat tagggtttct caaagtagga aaacgagcaa cgatccttag ttttgtgaat
    87601 gttgtatgtg tatcggtgat gacgatgctg cttccagtcg gacagatcac tgataatatt
    87661 ttgatgaatg ccctagtagg cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa
    87721 atgggcttta caactggtgg gatggatatc atctctcttg tactttcaca aacaactgga
    87781 aaaacagtcg gaaactacat gctgattcta aatggtatca ttgtagtcat cgcaggcttc
    87841 ttctttaact gggaaagtgc cttgtatacg attatttcca tttatgcaat gactcaagta
    87901 attgatgcca tccacacgag tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca
    87961 gaagaggtaa cagaagaact agcaaaacgg atggtacgtg gaatgacatt actgccgtct
    88021 gtcggtggct attcaaaaaa acaagggaac atgatcatga tggtcatcac ccgctacgaa
    88081 atgtatgatc ttgatcagat cgttcatgaa atcgacgacg atgcatttat caatatttta
    88141 ccaactcatt cagtatttgg ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca
    88201 actggcgtat tgccagaaat aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt
    88261 ttagaataaa tttaatctat tacatctaat aagaacattt ttataaattt aaacgagaat
    88321 cttacttcta aattctcatt tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt
    88381 gttaaggagt gtcgtacgat gagcgaagca aaccaagaac ggtctggaca agaaatagaa
    88441 ctaatcaaag aacgtattct tgctgccttg gaaatggtga ttgatcctga attaggaatc
    88501 gacatcgtca atttaggctt gatttatgat atagaattca atcctgaaaa tggagaaaca
    88561 gtcattaaga tgactctaac aactatgggc tgtccattag cagatatttt aacagaatct
    88621 atccatgacg cattaaaaga agtgccagaa gtaacgaaag cagaagtcaa actagtctgg
    88681 tatccagcat ggacgacaga taagatgagc cgttatgcac ggattgcgtt ggggatacga
    88741 tagaaatctt ggctgactag ctatgagaag caaataggaa aaacaaaaaa gccttttatc
    88801 ctttctagaa gaaaagagat aaaaggtttc tttgtttata aaattttgtc tgctatatta
    88861 taagttaatt aaataaatgt gttctattga taaaggacat tcaaatccct ttccttttgg
    88921 aatccttctt ttttacatca gtttgatttt cgtatagtat tctttctcat cttcacgata
    88981 aatcgcagcc ccattttttt gttgtgcttt aagactgcca agattttttt tatttacaga
    89041 caaaagtgcc tcatcaatgc cgattttttt tgcctcttgt agtactaatg ctaatccttg
    89101 tgtggcatat ccttttttac gataattagg ggagatgccg taaccaatat ggccagcgcc
    89161 gtttcttaat gtttcgttca gtttatggcg caaattaaaa attcctacat actcatgttc
    89221 atcactttct aaaataaaga cggttgcggg aacgtaactc tcaggaagat cgattcctaa
    89281 agacgttttt tcgcattttt ttacataatc taaaaattca gcaaatgtat agccataagc
    89341 attgttgata aatccactct catttggtcc aaagctagtt tgtagctgat aagctttctt
    89401 taaatctgct tcccatagtt tgatcagttt catcgtattc acctctttaa gtaaccttat
    89461 tcatttgttt tctactatag cactattgtt gtaaatagcc atgcctattt ttatttattg
    89521 tgtcatatcg tattaagaat catcgaacaa tacaaatatg aattacaata aaaaaattat
    89581 ttgaagttcg ttcattcgtt acttatgatc tctagaagaa agaaaacgtg aattacttca
    89641 gcgtctagaa ttccagaagg atagtaatca acagaagaaa taatcaatat ccaaatgaaa
    89701 gaagatagct gaatcgagaa aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta
    89761 ctaatcccag agtcgttcgt ttaacattga aatcgatcat atggataaaa cagatttttt
    89821 caccaaccct caatagatga tggagttgct cggatgaatt atttgtgtag atggcataag
    89881 ggagggcgta cagaaatgaa aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat
    89941 cctaaactaa aagatgtgat gcggctatgg cggaattggc agacgcgcta gcttcaggcg
    90001 ctagtgaggg tgacctcgtg gaagttcgag tcttcttagc cgcataattt gtttttttat
    90061 aaagagaagg tacacctgga gcgatgagaa agaaaataac atattatcta aaagaatata
    90121 ggaaaaattt taaacaacgg aaccagattc aaataaatca aaaaaaattt gtgtgaaggt
    90181 gggacagttt aaaacctaat agattgattc aaatagtaga tttatagatt ctgatacaag
    90241 aaatactggc aaacgatagt taaggaataa agactaaact taaactcaaa aagaggtcat
    90301 gcgaaagaat aaatcgcatg acctcttact atattttgaa tgtgtttaaa cgttcaaata
    90361 atgaatctta gtaccaaccg tttgcttccc agaatgcttt cgccgcttcc caagaaccat
    90421 aacgtgaagt aacatattgt tctgctactc tttcttggtt tgcagcagag tagtcaccat
    90481 ttaagtaaga agaatctaat tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag
    90541 aaccgcttga ttctttttgt gcgatccatt cttttgcaga gcttgtactt gctggagtag
    90601 cagcagtttc tgttacagca ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt
    90661 ccgtttgaac cgtttcttgt acaacaggtt gttcagcttg aacaggttca gcttggactg
    90721 gtgcttcttg aacggctgct tgaggttcag cagctgcttg tgcagagcct tctgttggga
    90781 ttgttaattg ttgtcctgag tagatcaggt taatatcaga aattgaattt gattctgcaa
    90841 tggcattgat caaggagtta tcaccaacgt atttttgaga aatcgttgat agagtatcac
    90901 ctgattgaac ggtataagct tcgtccgcat gagcagtcgt tcccatgaag aatgcagcgc
    90961 cggcagccaa agttgttcca aaaagtaaag ttttaagtga tgtcatgaaa aatctccttt
    91021 attcgttaaa aagttttatt tgttgtgttc aacgaggaat actttaacac gtgaaaatat
    91081 ttcataggta aaagaagcat gacaatttat gacaaatcca cgttttttgt aacagaatga
    91141 tgttcgctcg atgtgaaaaa tgatgaggaa tttcacataa tgcagaaata atgggattag
    91201 aacaggtgtt ctgatgagaa aagaaatgta taaataactt gaaagaaatt gcttttgttc
    91261 atcattttat ttacatattg cgtatatttt ttgtattttt gcttgagatg ttacaataaa
    91321 agaaaaagga ggcggttcca atgagtaaaa gataccagaa catcatggta gcagttgacg
    91381 gatcaagaca atcgatcaaa gcgttcgctg aagcccttga cttagcaaag gacaatgaag
    91441 cccacttatt tatcgtgtca atcatcaaca aagttgagct gacccatagt gcctatgcct
    91501 tttcaaaaat ctatgcagat gaaaaacaaa gaacagaagt agccatgctg aaaaaaatca
    91561 atgatgccaa agagtacggc attgctgaga tccacgcaat agttgaaact ggagatccaa
    91621 gaaatttgat tgccaatgtc atccctggac aagaaaatat tgatttgatc gttatgggag
    91681 cgacaggaaa aggtgctata caacaagcat tagttggatc gaccgcttct tatgtcgtat
    91741 cacatgcccc ttgcagtgtg ttggtcgtga agtaatgaga taagggagga atagaaaatg
    91801 gttcgtcctc aagaagtccg tgcgccaaaa gaaaaaatag aaattttagc aattattgaa
    91861 gacggcacac aaacaaagaa gggatactcg atcgctttag taaagtggaa aggaaaaaaa
    91921 ggtgttgctc tgagatggga tggagataac caacaagata aaggattccc tgtcacagct
    91981 aacggttatc atccagcgtg gtttgttctg cctgataaat ttatggaatt atacagctat
    92041 gattatgcaa gagccgcagc atcaatcaaa gcattagata aattaacaga agagcagaat
    92101 gaaatggagg aaaaataaaa aagacgaacc aatcagcctt tagaaaactg attagttcga
    92161 ggagtaaaaa tgaaaaaagg ttatttggga tgaaactaga ataatgcgta taacttaaat
    92221 aaaacttaaa gcaataaaat ggcaagaggg tgaggcaaat ggaagcagga aaagtattac
    92281 aggaagtaaa agcactatgt gcagataaaa aatatgaaga agcgaaaagc ctgatcgaaa
    92341 agaacaaaga aaatttggaa gaaaagtacc cgatcgcaca agaatttata gaattaaaac
    92401 aaggaacgat ccttcaccga ttcaaacatt tttttggtat aacagaatag aaaaaacgat
    92461 taaagatcgg cagaaatttg ctgcatcttt aatcgttttc tttttaaaca tgcgagggat
    92521 tcaattctga ttcttatata aaaaatagat ccaaataata agaaaaatta ctgaaagcgt
    92581 caattcgatg atgtgtgtta ttaatacaga cgttgtttcc caatgcctaa aagaaaaaat
    92641 ttgataagta aaatatacag caggtaaaga ggccagtaaa actgaaaact tcttttttgt
    92701 ccattttatg taatagaaaa gaatgcttat cataataaaa aaaggaatat aaaaaacaaa
    92761 ttgaccgatc gcgatcatac tattgcctcc gataaataaa gtccatgata caaaataaca
    92821 ctttgtgcga aatggcgcaa gcagttttgt ggatttatcc ggtaaaatct gtatcgattt
    92881 tgaaattaga ggagaaactt gctataatca aagtgaaaga aggagtggga ttttcatgga
    92941 tcatgtagtg ttagaaatcg atttgcattt gatcaataat attcgggtga tttactatgt
    93001 tgtaagtaac agtgtcgaac aacgtgtact gacgaataaa ataaatggta ttttagcaaa
    93061 gaaagacgtt caccgattca ataatggtga aggaagttat tatagtatcc cagtagaaaa
    93121 aattatttat acgacagtca aagtcagaga agatctagat accaaaaaag catatgaacc
    93181 tatttttaca acttattgat tgacagcaga tggaggaaaa aaatgaatcc atatgaacaa
    93241 cttattcaag atttgatcga tggaaaaatc gaaaagattg atgtaaaaag agaagaatta
    93301 atggcttttc gagaagcgtg gctgaagttg gagaatcgta aatatgtggt gggaaaagcc
    93361 tcactgaacg gcaatgtgac ttatcattac gatccgacac gtttatttta gtatgaagta
    93421 ttcagaaaag aaccaaagga ggacgatgca gcagcattgt cctcctttga ttttacaaaa
    93481 gtgaaagttt tacttatttg aatagatttt taaatttgtt tttgacactg tcgatattta
    93541 catcttttaa aagattttgg gctttctctt tataaggtcc taattgttct ttatgttctt
    93601 cgataaatcg ttttgcgcca tcgaaattgc cgttcttaat catttccttg actttttgaa
    93661 ataattcttc ttgattcatt ttgaacacat cctttgctat caacatgatt ttttacatgg
    93721 acaaaacgag aaaaactgat ttatccacac gtcaataata tcaaataacg atcattttta
    93781 ctaaaaaaac actcgattct cgcttctaaa gtaggagaaa aatctccaac ttgggacgga
    93841 tggaatttct ttaactgacc catatactga atatataaca aggaggtaga aagatgaaag
    93901 tgattaaata tttgattgga ctggcggcga ttagtggtat tttgctattt ggactgaaat
    93961 attatactga aggaagttat ggagaaatcc ctggtatact tgatcagcta aatccattag
    94021 ttgaaaaagg agaggtctat gtaaagacac aaaaaccaga ggaagtaaat gaatatggta
    94081 cagcaaggta tgtgcagaaa gctgctgatg ccaatgggaa agaacgaacg gttgaattta
    94141 atggtctttc tgtgttaaaa gaaaatcatt atttgaaaat tagcaataaa ggagcacaca
    94201 tagaaacgta tgaggaagtt cctagagaaa aagttccgga gaaagcatta gcaattattg
    94261 ggtaaatcag tttttgtatt aagaatacgc ccgattttat tattcataat ttaattatgt
    94321 aaactaaata caagtagtaa aaataataca ataatccccc tgcaaaaaga ttgttctttt
    94381 acagggggtt cattttttca gcaagcacat caaattggga tggcagggtt cgaacctaca
    94441 attgcttgat tcagagtcaa gtgccttacc agtttggcta catcccattt attcaacaaa
    94501 ataatcatag cactttccta aaaaaacgca agttattttt cttgatcgga tagttgttgc
    94561 tttactcgta aaaggcggcg tactagtttc ttttctaatc cagcaggaag ggcaatcagt
    94621 tctgctactg acaagacggt gtccaataac tgatattctt tgacaggttc attcataata
    94681 tgatagatgt ttgctaaacg ttcaatatat tcaaagcgag tatactctgt tcgagcaatt
    94741 agaaatttca ataaatcggt ggctttctcg tactcttgat ctgcgtaata aacaagagat
    94801 tgacgataca atttgatttc cagttcattg agggtagcag gtctgcgacg ctcgttccac
    94861 tcattaatag agactttacc ttgaacattt cccttgcagt cacggacaaa aataatcgga
    94921 atttcatttg gatccatttt tttatgtgtg aatttttcca ttatctacgc tcccttcaaa
    94981 ggtttggtat cttgagtata agggattttt gtttgaatta ctagcttttc tccaaagtta
    95041 gcatctgtcc acccattgca gtgaaaatcg ccgaaaaatc gttgatcggt actgctcgat
    95101 tttttacacc atagggatcg ttgacataaa tattttcttt gtcatatcca gtgactacac
    95161 aagcatgaac aagaggagtg actcttactt ctccattatt tgtggtccaa gtcataaaat
    95221 cagtttcatc tgggacttca aaattgatag tcacagaagt ccagacagga tttcctttag
    95281 atagtgcttt tttgatatca tcgagttgag catcggtttt tggaaaagtg tgataatccg
    95341 tttggacgat ttgctgagca agggaggcaa ctgggggaac atagacgacc atagcccagt
    95401 ctccgccact gatatcacca acaaatcctt cattaggatc gccatgagtc ccatctgcat
    95461 tgaaaacagg gacgtaatcc aattgctcag ctaactggtt tttagttgtt tcataaccat
    95521 aaaaagataa aagcatagaa agcgaagtga cttcacagcc attttctaaa gaaggagaat
    95581 caaactggct ctctaatgga acatctagta aaacttctgc tttctctttt gtctttttcg
    95641 ttttttgtgt tgaacttttt tgtgcagtgt tcattgtatg ttctggagaa tggatatgta
    95701 ggtaataact tgtaccgctt aataaaagag cagctgtgcc ccctagaaac aatagttttt
    95761 tgaacgtttt tttcttcatt tgaaatctcc cttttcagct gtttatcttt cctattataa
    95821 atgtattttt attggaaatg gaagtattat attttaatat gtaggaaata attagttttt
    95881 aaggaatatg ttttaatttt ttgaaaaaaa ttcttctaaa tacaacagct gttttaattg
    95941 tgaaaacgaa aacagtacgc tataatgcct gtaaggttga tttttaactg taaataaaaa
    96001 tcaatgagtt aaatctccaa agaaatagca gtggtaatgt ggagaaaagt tccagcagtt
    96061 taggaggatt ttgatgacgg aagaaatgat taatttagga gaacaatatg catgcaaacc
    96121 aattggtttt acaaaaacgg tcattggtga agttgtatca aaaatgacaa actgcgccgt
    96181 ggtcaaagtt gcccaatgtg ctgcagagga ccaagaatta ttagatgaaa aagcaagcat
    96241 ggttgttgca aaatatgata catttgaata aagaaaacaa ttggacgtgc ggcgtgctgc
    96301 aagtccaatt tctttgagaa ggtaaatcag tattcttttc ctattttctg gagaagaaaa
    96361 agcgaggcga gttgtcatgg taacattctt tacggttcta ttgtttttat tcgttgttgt
    96421 gatgttttta tttctttggc tggttcgaaa agaaattatt tatcggactg tgcgaaatcg
    96481 atgggtctac ttggtgatac ccttcttagt gtcactagtc atttggtata cactcatcag
    96541 tcatcctaca gcagatgaat tggcaaaagg gatcttgtca gcaatgattt ttattagttt
    96601 tttgttggac agtagaggaa tcacagaaga aggacttgtt ttgaatagct ttgacaaaaa
    96661 aggtgttcct ttttctgaga tcaataaaat tgtattatat cagccaaaag gttctaaaat
    96721 cgtaaaaatg aattttttcc gaaacggatg gcgaggaccg atgctgaaat tttctgcatc
    96781 attagaagaa ttagttccat ttttgtccca acgcttaaac gatgaggcag agattgatat
    96841 tatgattgat ccagaataaa aacagaggtt gcgacaagat gtttgtcaca acctctgttt
    96901 tttatttcaa ataaaattat ccaaatacgc ggaagcgatc ggcatcgttc atgtattctt
    96961 tttccccagc cggtgtttct ggtgaaccga atactaattg cgaacgtagt ttccagtttg
    97021 aagggatatt ccattctttt gcaactgctt cgtcaataac tgggttgtaa tgttgcaagt
    97081 ttgcgcccaa tcctttatca actaatgcta cccaagtatt agctgttgcg attccgtttg
    97141 attgttctga ccaatctggg aagttgtctg catataattc aaattgttct tgtagacctt
    97201 ttaccacgtc agtatctttg aagaataata ctgtaccata accatttttg aaaccagcta
    97261 atttgttttg tgtatttggg aaagcttctg ctggagtcaa tgggcgaagt gcttcttcag
    97321 tgatttccca aagtttttca tgtgcatctc cgaaaaggat aactgcgcgt gttgattgag
    97381 cattgaaagc agttgggctt tctttaattg cttctttgat caaagttgtt aattcttcat
    97441 tagaaagtga aacattacgt cctagatgat agattgaacg gcggtttttc aaagtgtcag
    97501 taaaagttgt cataataaaa tttctccttt gatttgcgtt ttgcattttt ttaattgatt
    97561 acgaaatgaa gtatagcacc ttacaaaaag taagtaaaca gatatgctca caaaaaataa
    97621 gcgaagttca aaaaacaatt ttaataagaa aaatcgttat gtaaggattt tcttagttat
    97681 cgctttcatt gttgtgtttt aggaattttt gcttatttga aaaaaacagc tgatatataa
    97741 ttattaaatc tctatcttct tttttttatg atattgccat taaaaattac ttgcttttat
    97801 agaaaattag tttatatttc ttaaataagt tttttagtta aatttttcgg taaagatgta
    97861 taatattttc tgaaaattta aagaattggg gtatagatta tggaaaaaca gcaagacaag
    97921 ggcttcttcg ggcagccgaa gggattgtca actttgttct tcactgagat gtgggaacga
    97981 tttagttatt acggtatgcg tgcattactt atctattata tgtacgatac agttgccaac
    98041 gggggacttg gattaccacg agcaacagca ttagctatca tgtcgatcta cggatctttg
    98101 atctacatgt ctagtatcgt cggcggatgg gtatctgacc gagtgttagg agcaaaacgc
    98161 actgtattct tcggtggagt tttcattatg cttggccaca tcgtcttggc tttaccattt
    98221 ggtgtaagta cattgtttgt ttcgatggca ttgattatta tcggaaccgc ctttttgaaa
    98281 ccaaatgttt ctggcatggt cggacatctt tattcaaaga ctgacttgcg aagagatgca
    98341 ggattttcga ttttttacat gggtgtcaac ttaggggcat tattagcacc actaatcgtt
    98401 ggtacaatcg gtcaagaata taactatcat ttagggttct ctttagctgc tattgggatg
    98461 ttctttggat tattacaata catcatccaa ggacgcaaga cattagacgg agtgggtaca
    98521 acaccgccga atcctttgac aactgaagaa cgtaaaggat tcatccgtaa cttgatcatc
    98581 gcacttgttg ttatcgctgc agtattcggc ggggcacaac taactggtca tctgacaatt
    98641 gacttttttg tcaatacaat cagtgtattg gggattatct taccgttgta ttactttatc
    98701 aaaatgctga cttcaaaaga tgtaactgct gaagaaaaac caagagtatg ggcttatatc
    98761 ccattattcc ttgcagcaat cattttctgg tcattacaag aacaaggttc atctatctta
    98821 gctttatttg ctagtgaacg tacacaatct acgctatttg gtgtaacgat ccaagcaagc
    98881 tggtatcaat cattgaatcc aattttcatc tttattctga caccagtatt cgtaacgatg
    98941 tggacgaaac ttggtgaacg tcaaccatct acagtagtga aattctctct tggactttta
    99001 tttgcaggat tatcgtactt gctattgatg ttgccgggta tgctttatgg tacaagtgga
    99061 aaagtaagtc cattgtggtt gttgggaagt ttcttcatcg tggttattgc tgaattgtgt
    99121 ctttcaccag ttggtttgtc tgtgacaaca aaattagcac cacgagcatt tgaatcacaa
    99181 acaatcgcta tttggttctt agctgatgct tcttcacaag caatcaatgc acaaattgca
    99241 cgcttctaca caccgggaac agaatctgct tacttcggta ttatcggtgc agtagcaatc
    99301 gtcggtggta tcttgctatt tactgtgaaa gaaccaatca aaaaactaat gggctctatt
    99361 cattaatcaa tgaacataaa aacaggaatt gcttatttgc agcagttcct
//
LOCUS       DASHRQ010000002        83161 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.002, whole genome shotgun sequence.
ACCESSION   DASHRQ010000002 DASHRQ010000000
VERSION     DASHRQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 83161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.002"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            212..604
                     /locus_tag="U2M88_000102"
     CDS             212..604
                     /locus_tag="U2M88_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEM8372518.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="U2M88_000103"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="U2M88_000103"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEM8372519.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="U2M88_000104"
     CDS             3165..4775
                     /locus_tag="U2M88_000104"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEM8372520.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="U2M88_000105"
     CDS             5067..5936
                     /locus_tag="U2M88_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEM8372521.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="U2M88_000106"
     CDS             6101..7372
                     /locus_tag="U2M88_000106"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEM8372522.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="U2M88_000107"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="U2M88_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEM8372523.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="U2M88_000108"
     CDS             8763..9026
                     /locus_tag="U2M88_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEM8372524.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="U2M88_000109"
     CDS             9471..9920
                     /locus_tag="U2M88_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM8372525.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="U2M88_000110"
     CDS             9977..11395
                     /locus_tag="U2M88_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM8372526.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11379..12254
                     /locus_tag="U2M88_000111"
     CDS             11379..12254
                     /locus_tag="U2M88_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003733012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEM8372527.1"
                     /translation="MRWKRNVNTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAER
                     LEAPVILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYS
                     SVMIDASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPD
                     QAKIFVKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNP
                     DAEVSEAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVK
                     HKMEILNTVGKASLY"
     gene            12428..13202
                     /locus_tag="U2M88_000112"
                     /pseudo
     CDS             12428..13202
                     /locus_tag="U2M88_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            13349..13714
                     /locus_tag="U2M88_000113"
     CDS             13349..13714
                     /locus_tag="U2M88_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEM8372528.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="U2M88_000114"
     CDS             complement(13862..15070)
                     /locus_tag="U2M88_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM8372529.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="U2M88_000115"
     CDS             complement(15089..15367)
                     /locus_tag="U2M88_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372530.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="U2M88_000116"
     CDS             15521..16216
                     /locus_tag="U2M88_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEM8372531.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="U2M88_000117"
     CDS             complement(16658..17455)
                     /locus_tag="U2M88_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEM8372532.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="U2M88_000118"
     CDS             complement(17452..18636)
                     /locus_tag="U2M88_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEM8372533.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="U2M88_000119"
     CDS             complement(18661..19908)
                     /locus_tag="U2M88_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEM8372534.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="U2M88_000120"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="U2M88_000120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEM8372535.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="U2M88_000121"
     CDS             21888..22202
                     /locus_tag="U2M88_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEM8372536.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="U2M88_000122"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="U2M88_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEM8372537.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="U2M88_000123"
     CDS             22887..23531
                     /locus_tag="U2M88_000123"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEM8372538.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRIDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="U2M88_000124"
     CDS             23546..23875
                     /locus_tag="U2M88_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEM8372539.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="U2M88_000125"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="U2M88_000125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEM8372540.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="U2M88_000126"
     CDS             24863..25687
                     /locus_tag="U2M88_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEM8372541.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="U2M88_000127"
     CDS             25680..26027
                     /locus_tag="U2M88_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEM8372542.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="U2M88_000128"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="U2M88_000128"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEM8372543.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="U2M88_000129"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="U2M88_000129"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEM8372544.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="U2M88_000130"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="U2M88_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEM8372545.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="U2M88_000131"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="U2M88_000131"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEM8372546.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="U2M88_000132"
     CDS             31657..32451
                     /locus_tag="U2M88_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8372547.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="U2M88_000133"
     CDS             32441..34483
                     /locus_tag="U2M88_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8372548.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="U2M88_000134"
     CDS             34764..34913
                     /locus_tag="U2M88_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEM8372549.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="U2M88_000135"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="U2M88_000135"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEM8372550.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="U2M88_000136"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="U2M88_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEM8372551.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="U2M88_000137"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="U2M88_000137"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEM8372552.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="U2M88_000138"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="U2M88_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEM8372553.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="U2M88_000139"
     CDS             39330..40412
                     /locus_tag="U2M88_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEM8372554.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="U2M88_000140"
     CDS             40412..41215
                     /locus_tag="U2M88_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEM8372555.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFVFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="U2M88_000141"
     CDS             41285..42025
                     /locus_tag="U2M88_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEM8372556.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="U2M88_000142"
     CDS             42036..43040
                     /locus_tag="U2M88_000142"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEM8372557.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="U2M88_000143"
     CDS             complement(43085..44929)
                     /locus_tag="U2M88_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372558.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45141..45659
                     /locus_tag="U2M88_000144"
     CDS             45141..45659
                     /locus_tag="U2M88_000144"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEM8372559.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45787..46470
                     /locus_tag="U2M88_000145"
     CDS             45787..46470
                     /locus_tag="U2M88_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEM8372560.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46779..46852
                     /locus_tag="U2M88_000146"
     tRNA            46779..46852
                     /locus_tag="U2M88_000146"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46813..46815,aa:Arg,seq:tct)
     gene            47011..47370
                     /locus_tag="U2M88_000147"
     CDS             47011..47370
                     /locus_tag="U2M88_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEM8372561.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47493..49124
                     /locus_tag="U2M88_000148"
     CDS             47493..49124
                     /locus_tag="U2M88_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEM8372562.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49186..49827)
                     /locus_tag="U2M88_000149"
     CDS             complement(49186..49827)
                     /locus_tag="U2M88_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEM8372563.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    50013..50089
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            50109..50417
                     /gene="rplU"
                     /locus_tag="U2M88_000150"
     CDS             50109..50417
                     /gene="rplU"
                     /locus_tag="U2M88_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEM8372564.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50444..50788
                     /locus_tag="U2M88_000151"
     CDS             50444..50788
                     /locus_tag="U2M88_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEM8372565.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50801..51094
                     /gene="rpmA"
                     /locus_tag="U2M88_000152"
     CDS             50801..51094
                     /gene="rpmA"
                     /locus_tag="U2M88_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEM8372566.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(51187..52335)
                     /locus_tag="U2M88_000153"
     CDS             complement(51187..52335)
                     /locus_tag="U2M88_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM8372567.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(52347..52658)
                     /locus_tag="U2M88_000154"
     CDS             complement(52347..52658)
                     /locus_tag="U2M88_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372568.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            complement(52711..53106)
                     /locus_tag="U2M88_000155"
     CDS             complement(52711..53106)
                     /locus_tag="U2M88_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEM8372569.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            complement(53142..53486)
                     /locus_tag="U2M88_000156"
     CDS             complement(53142..53486)
                     /locus_tag="U2M88_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8372570.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            53787..54008
                     /locus_tag="U2M88_000157"
     CDS             53787..54008
                     /locus_tag="U2M88_000157"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372571.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            54135..54911
                     /locus_tag="U2M88_000158"
     CDS             54135..54911
                     /locus_tag="U2M88_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEM8372572.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            54914..55102
                     /locus_tag="U2M88_000159"
     CDS             54914..55102
                     /locus_tag="U2M88_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8372573.1"
                     /translation="MSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRRKP
                     DKQIKPIEAALAELEKEK"
     gene            55104..55388
                     /locus_tag="U2M88_000160"
     CDS             55104..55388
                     /locus_tag="U2M88_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372574.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            55409..55597
                     /locus_tag="U2M88_000161"
     CDS             55409..55597
                     /locus_tag="U2M88_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372575.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            55923..56162
                     /locus_tag="U2M88_000162"
     CDS             55923..56162
                     /locus_tag="U2M88_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372576.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            56168..56854
                     /locus_tag="U2M88_000163"
     CDS             56168..56854
                     /locus_tag="U2M88_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372577.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            56857..57687
                     /locus_tag="U2M88_000164"
     CDS             56857..57687
                     /locus_tag="U2M88_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEM8372578.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            57704..58555
                     /locus_tag="U2M88_000165"
     CDS             57704..58555
                     /locus_tag="U2M88_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM8372579.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            58552..58911
                     /locus_tag="U2M88_000166"
     CDS             58552..58911
                     /locus_tag="U2M88_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HEM8372580.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            58926..59087
                     /locus_tag="U2M88_000167"
     CDS             58926..59087
                     /locus_tag="U2M88_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEM8372581.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            59084..59389
                     /locus_tag="U2M88_000168"
     CDS             59084..59389
                     /locus_tag="U2M88_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372582.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            59389..59745
                     /locus_tag="U2M88_000169"
     CDS             59389..59745
                     /locus_tag="U2M88_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HEM8372583.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            59732..59950
                     /locus_tag="U2M88_000170"
     CDS             59732..59950
                     /locus_tag="U2M88_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372584.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            59971..60060
                     /locus_tag="U2M88_000171"
                     /pseudo
     CDS             59971..60060
                     /locus_tag="U2M88_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            60135..60602
                     /locus_tag="U2M88_000172"
     CDS             60135..60602
                     /locus_tag="U2M88_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEM8372585.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            60877..61113
                     /locus_tag="U2M88_000173"
     CDS             60877..61113
                     /locus_tag="U2M88_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372586.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            61137..61478
                     /locus_tag="U2M88_000174"
     CDS             61137..61478
                     /locus_tag="U2M88_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372587.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(61471..61662)
                     /locus_tag="U2M88_000175"
     CDS             complement(61471..61662)
                     /locus_tag="U2M88_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEM8372588.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDMTK"
     gene            61872..62519
                     /locus_tag="U2M88_000176"
     CDS             61872..62519
                     /locus_tag="U2M88_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372589.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
     gene            62779..63123
                     /locus_tag="U2M88_000177"
     CDS             62779..63123
                     /locus_tag="U2M88_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEM8372590.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            63128..63409
                     /locus_tag="U2M88_000178"
     CDS             63128..63409
                     /locus_tag="U2M88_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372591.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            63512..63826
                     /locus_tag="U2M88_000179"
     CDS             63512..63826
                     /locus_tag="U2M88_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEM8372592.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            63804..65498
                     /locus_tag="U2M88_000180"
     CDS             63804..65498
                     /locus_tag="U2M88_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEM8372593.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGIVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            65518..66696
                     /locus_tag="U2M88_000181"
     CDS             65518..66696
                     /locus_tag="U2M88_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEM8372594.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDMI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            66659..67345
                     /locus_tag="U2M88_000182"
     CDS             66659..67345
                     /locus_tag="U2M88_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEM8372595.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            67345..68505
                     /locus_tag="U2M88_000183"
     CDS             67345..68505
                     /locus_tag="U2M88_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEM8372596.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            68515..69390
                     /locus_tag="U2M88_000184"
     CDS             68515..69390
                     /locus_tag="U2M88_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372597.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            69387..69698
                     /locus_tag="U2M88_000185"
     CDS             69387..69698
                     /locus_tag="U2M88_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372598.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            69688..70041
                     /locus_tag="U2M88_000186"
     CDS             69688..70041
                     /locus_tag="U2M88_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEM8372599.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            70031..70432
                     /locus_tag="U2M88_000187"
     CDS             70031..70432
                     /locus_tag="U2M88_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372600.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            70425..70829
                     /locus_tag="U2M88_000188"
     CDS             70425..70829
                     /locus_tag="U2M88_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372601.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            70841..71446
                     /locus_tag="U2M88_000189"
     CDS             70841..71446
                     /locus_tag="U2M88_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEM8372602.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            71466..71828
                     /locus_tag="U2M88_000190"
     CDS             71466..71828
                     /locus_tag="U2M88_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372603.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            71831..72013
                     /locus_tag="U2M88_000191"
     CDS             71831..72013
                     /locus_tag="U2M88_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372604.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            72030..75449
                     /locus_tag="U2M88_000192"
     CDS             72030..75449
                     /locus_tag="U2M88_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEM8372605.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDALSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            75500..76237
                     /locus_tag="U2M88_000193"
     CDS             75500..76237
                     /locus_tag="U2M88_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEM8372606.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            76247..78537
                     /locus_tag="U2M88_000194"
                     /pseudo
     CDS             76247..78537
                     /locus_tag="U2M88_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            78561..80651
                     /locus_tag="U2M88_000195"
     CDS             78561..80651
                     /locus_tag="U2M88_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372607.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGKPNLMSKLKSSDFN
                     VGYHGSLTLDNEKLHFTSDGTGSIIMFTRINTPQLTSGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDAPYYLSKVALGENLIKPESQQPVTNSNY
                     LINTYNIKPMVKGKKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTNVWS
                     KTFTAADDSHPTTPQVQIYQVPSTSVGQCTIKWLKLEEGNTRTPNISEYKYRGTGMRD
                     SNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTT
                     AKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFV
                     ALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNS
                     SYGSDYTFAITYTI"
     gene            80668..80817
                     /locus_tag="U2M88_000196"
     CDS             80668..80817
                     /locus_tag="U2M88_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372608.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            80814..81260
                     /locus_tag="U2M88_000197"
     CDS             80814..81260
                     /locus_tag="U2M88_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372609.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            81262..81399
                     /locus_tag="U2M88_000198"
     CDS             81262..81399
                     /locus_tag="U2M88_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEM8372610.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            81437..81730
                     /locus_tag="U2M88_000199"
     CDS             81437..81730
                     /locus_tag="U2M88_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372611.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            81727..81951
                     /locus_tag="U2M88_000200"
     CDS             81727..81951
                     /locus_tag="U2M88_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEM8372612.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            81948..82973
                     /locus_tag="U2M88_000201"
     CDS             81948..82973
                     /locus_tag="U2M88_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEM8372613.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
BASE COUNT        27785 a        13702 c        17149 g        24525 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 atacaatgaa agagttattg aaagtggcta aagagaatca atttgcagta ccagccttca
    11461 atatttgcag ctatgatatg atgaaagcaa tcatcgaaga agcagagaga ttagaagcac
    11521 cagtaatctt agaaattcat cctgatgaaa tcgcctattt aagtgatgag tttgttgcgg
    11581 cagtaagagc ctatgcttat acaagtaaac tgccgatcgt tatccacttg gatcatggcg
    11641 gtacgataga agatgtctta cgggcgattc gaaacggcta ttcttccgtg atgatcgatg
    11701 cgtctttgaa atcattcgac gacaatgtac agattacgaa aaaagtggtt gcgttagctc
    11761 atgcggtaaa tgtctcagtg gaggctgagc ttggaacgat tgggaataat ggttctgctg
    11821 aaggaggtgc tgaaacaatc atttatacag atcctgatca agcgaagatt tttgtgaaag
    11881 aaacaggaat cgatacactt gctgtagcaa ttggaacagc gcatggcttg tatccgaaaa
    11941 acagaactcc aaaattaaat atcgacttac tgaaagaatt gaaccaacaa atcgatcttc
    12001 catttgtttt acatggcggt tcaggtaatc cggacgcaga agtcagcgaa gcagtaaaat
    12061 acggagttgc caaagtgaat ttgagctcag atcttaaaag cgtctttttc aatgctttga
    12121 gagaaatatt gagtgaaaat ccatcgatgt acgagccgaa tgcaatttat ccagcagcta
    12181 ataaaaaggt acaagaagtg gtcaaacata aaatggaaat tttgaacaca gtaggcaagg
    12241 ctagtttata ctaaaaataa aaatgtgtgg tcaagagggt atgacaatag tcttgtcata
    12301 ccctcttaac cacacacgat gttcagaagt agttttttcg aaaaatttct tcttatttct
    12361 cgaagccaat cacttctgtc acaacctctt ttttcctgtt actatataaa gtaaatgggg
    12421 tgattttatg ttgaatgaag tggagaatag acagcaggag attgtgaatt atctaaaaaa
    12481 caaccaattt gcaagagtca ccgatttgat cgagttagta aattatagcg aagccacgat
    12541 taaacgtgac cttgtttttc tggaaaaaag tggattgatc cgtagagttc gcggtggcgc
    12601 catgcttatt gacaatcgaa aaattgacgt gccttacctg atgaagatca ccaaattaga
    12661 agaagaaaca gaaaaacgta agattgcaga tacagcttct tctttgattc gagatgatat
    12721 ggtcttattc ttggattcca gtacaacctc tttacactta gtcagaaacc tagcaagatt
    12781 tgatggtttg cagatcatta cgaatggcat cgtcacagct gctatgttga gtgaatatac
    12841 aacggcaaaa gtatctgtat tgggaggggc aattgttccg aaacgagcaa caatcaacgg
    12901 ttcaaaagcc tataacgatg cattgtcata ttatgcggat ttagcgataa tgagttgtcg
    12961 cgggctagat ttcagccaag gagtaacaga aactcatgaa ggagaggcaa tggtcaaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttttct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 ttttaaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tagatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtatga
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgt
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat
    45121 aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa
    45181 cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt
    45241 tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga
    45301 tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg
    45361 aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag
    45421 atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa
    45481 atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg
    45541 catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag
    45601 catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag
    45661 aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga
    45721 gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg
    45781 agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt
    45841 atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat
    45901 gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga
    45961 cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc
    46021 atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg
    46081 atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt
    46141 gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg
    46201 gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa
    46261 ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa
    46321 gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg
    46381 atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg
    46441 acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg
    46501 attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta
    46561 aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc
    46621 aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag
    46681 ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa
    46741 tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct cagctggata
    46801 gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa
    46861 ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt
    46921 ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa
    46981 ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact
    47041 ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac
    47101 acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca
    47161 ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct
    47221 tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt
    47281 gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct
    47341 ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag
    47401 gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt
    47461 ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa
    47521 aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca
    47581 cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg
    47641 cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg
    47701 gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg
    47761 caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg
    47821 cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg
    47881 ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg
    47941 ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc
    48001 cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc
    48061 caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag
    48121 ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca
    48181 gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc
    48241 cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg
    48301 gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa
    48361 atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg
    48421 tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac
    48481 tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag
    48541 gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta
    48601 ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct
    48661 ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca
    48721 aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag
    48781 aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt
    48841 cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct
    48901 ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag
    48961 aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa
    49021 aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg
    49081 cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac
    49141 agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa
    49201 aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa
    49261 aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc
    49321 atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt
    49381 aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa
    49441 ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga
    49501 acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg
    49561 atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt
    49621 taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac
    49681 atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag
    49741 aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc
    49801 ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat
    49861 aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt
    49921 attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt
    49981 ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta
    50041 ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc
    50101 tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac
    50161 aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag
    50221 ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg
    50281 ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac
    50341 ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg
    50401 acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt
    50461 taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg
    50521 tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa
    50581 cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg
    50641 ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat
    50701 tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat
    50761 ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct
    50821 acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc
    50881 aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta
    50941 ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac
    51001 tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca
    51061 agtgtctgtt tatccagtag ctcaagaagc ttaataaata aactgctcta tctcttgtgt
    51121 agcaagggat ggagcttttt tgtaaattat gggatttttt ttgaccatac ttttgaccat
    51181 actttcttag aagttaatat aatttgttag tttttgtgtt gtttcttctt ttttgctgtt
    51241 agtaatgtgt gtatatgtgt ccatagtagt ttttatattt gcgtggccca ttctttcttg
    51301 gacttctttg tgatctgcgc ctgcctcata tagcattgat gcatgtgtat gtctaaaacc
    51361 atgcagaccg atataaggta agccggcacg ctcacgatat atattgtatc tttctgccac
    51421 tgactgatta attgtgaaat tattttcgtc attggtaaac aaataattag ctttagtaaa
    51481 tcctaattga aataagtact ttctttgttc gagcttccaa aattttagtt gctttatagt
    51541 tttttcatct aaataaatta ttcgatttga ttttttggtt ttaggagttt cagatatata
    51601 atatttatct gaacgagcta cagttttttt aatggccatt tcatttgttt taaaattaat
    51661 attgtcccaa gtgagtgcta atatttcgcc gattctacat ccgctgaatg ctaatagtct
    51721 aaagagcgta tagtctctgt ttttaaaata tggattctgt tcttgagata cttcatttag
    51781 aaataattcc aactgttctt ttgtatataa ttttaatttc ttttctgttt taatttgtgg
    51841 ctttggaata agtaaattta atgttggatt atcagatgtt aaaccaatat ttatagcata
    51901 cttaaaaact ttattcatat agtttatgaa aagagggtac tgctttgaag ttcctttttc
    51961 actccaatca tttactacct tttgacaata tgccgtatta attttagaaa gcttaatatt
    52021 tccaaatttg ggatatatat gtttattaaa gattatttta gtccttgaat atgaactttc
    52081 ttttacagtt tttttgtagc tttctagcca taattctgct gcttctttga aagtagtatt
    52141 tgtacttgtg ggcattcctg ttttttgtaa ttctagttct aacctagaaa gtgctgtttt
    52201 tgcttctttt tgtgttctaa atcctcgttt agtagtatat tttttctttc cagttagcgg
    52261 atcaataccg agataagttt taaagtacca tgctttttcg ccatttttct ttttatattg
    52321 ttttatcatt gccatcaata ttacccctat tcaaattcta attctgccat gctatcattg
    52381 aaaaattcga tcagtccttg ttcctctata aattcaagcc atatttcctt cccgttaatt
    52441 aatggaactc ctttttcttt acgaattgtt tgatacaagt tagtcacgac ttgtttccac
    52501 atttctaata cagctttatc tattttctta tcatcccagc gttttttttg gggtctattt
    52561 ttgttttttt caatttgata attagtagct ttgatgataa atgtttcgta tccggttgtg
    52621 cttttttcta accattcttt gaattctgta tatatcattt ttgttcacct ttctattttt
    52681 gaacgtatgt tcttttttgc ttaaaaaatt ttagagagct gcataacaat agatgcgagc
    52741 ttttaataat tctttcacaa gtggttcgaa gcgaacgctt aaattgttat cttctataaa
    52801 tttcatgtaa ttgatattgt gtggttctaa ttcagacttt gcaatatatc tatctaataa
    52861 tttttcaatc atgaagcgat cagcctcata ttccattttt gaatggagag agaatgctaa
    52921 gttatataaa aaataattat catgatgttt agaagcgtgt cctaattcat gtaataaggt
    52981 tctttgtctt ctatacttag ataaattagc ttttattact atggtattca tacaagcaac
    53041 ataatggcca tcggcatcaa ggttttcacg ctcttctacc ttaacgccga gttcattaat
    53101 tatcatttca atttgactgt ccaaatgact cacctactta tttagttatc ttttttctct
    53161 aaatatgctt caatcatagc tgatagtatt tccctatcat tatcggtgag cggttttccg
    53221 tcgctactca taacagatgc taaagcttct tccacggtta gttttctttc ctctggtgca
    53281 actcccgcgt taggatcatc tgtgcgtcct agcaaataat ctgttgatac atggaagtag
    53341 tcagcaactt tttgcaaagt ttctatttta ggactttgtg tcttccattt ataaatagag
    53401 ttttttccaa atcctattct attttctaat tcagaaatag aaatacctct ttttttacag
    53461 agctctttaa ccctttcgaa aagcatgtca aatcaacctt cctagagcgt acgataataa
    53521 tttatacaaa aagatagaaa ggtagttgac aaatctatcc aattgtctta tatttaactc
    53581 gtaagctaat ttaataagct aacaagcaac aagaaataca cctaataaaa taaacgaatt
    53641 cgaggtcggc aaactaagaa tgttaatttt tagggttttt ctaggcttat ttaactgtat
    53701 ataaatataa gacagttgga tagaaatgtc aataaaaatt agctaatttt attagcttac
    53761 atatttaaag aaaaggagga aacatcatga aacaaaataa aaaaactcct tccgcaaaag
    53821 gagctttaat aactttaaat tttaaagacc aaaagtttag tgctgttgtt gatgaaattc
    53881 atccacaaga gcatcaaaaa aattcttttg caactgacga gaaaaagact cagaaacgct
    53941 ctgagaaagt tcttcgaata attcaatttc tgttggaaaa gattcgtcat tttggaagtc
    54001 tattttgaat tctttattaa cagtcttgaa tacggtttgt ttgaataagg cgtcgacatc
    54061 catattaaat tcttccatac tattttcacc tcgctttcta ttcaaaatta tatcaaagaa
    54121 aggaatgagc aaaaatgaac acaccgcaaa tttttaactt tgaacaacat gaagtaagaa
    54181 cagtaacaat acatgatgaa ccattttttg ttggaaaaga tgtggctaaa gttctagggt
    54241 accaaaatgg tagtagagat attaacagac acgtcgatgt agaagatcgg caaaactacc
    54301 aaaacggtac ttttgaatcg cctagagggt taactattat taacgaatca ggtttgtact
    54361 ccttaatttt aggaagcaaa caaccaaatg ctaagaaatt taaacgttgg gtgactagcg
    54421 aagtgttgcc agcaattaga aaacatggag gttatctaac tccagaaaaa gtagaagaag
    54481 ctttgcttaa tccagataca atcatccaat tagcaactaa gttgaaagaa gaacgtactg
    54541 gaagactaat cgcagaacaa aaaattgcag aatacgaacc caaaatttcg tatttggata
    54601 gtatcttatc ttctacagat tcagtaacta ttagtcaaat cgcggctgat tatgggatgt
    54661 ctccacaaca gatgaacaaa ctacttcata agctaggtat tcagaaaaaa gtaggcaacc
    54721 aatggttatt atgcaaaaaa cacatgagac agggttacac aaaatctcat acaactgaga
    54781 ttccgaaatc tgatggcgga acaaaagttg tgatgaatac aaaatggact caaaaagggc
    54841 gtctgttcat ttatgagtcg ctgaaaaaag aaggatatat tccagaaatc gatttattag
    54901 aggaggaatg acgatgtcac aagaatttat tttgaaagtc agaattcaat tagcaaaata
    54961 cggaaagtct cagaactggt tagctgatac tatcggaatt tctagaccat atatgtcgga
    55021 cattatgaat gggcgtagaa aaccagataa acaaatcaag ccaattgagg cagcactagc
    55081 agagttagag aaggagaaat aacatgcaaa taacaattcc agataattta gtagtttccg
    55141 aattaactac acagattacg aatgctgttc ttaattcatt ggacgaacga ttacacctta
    55201 tgaacaaatc agtagagctt cctccatatc caaacaaatc agaggtaaaa aaagttttag
    55261 gcattggtga tgacaaatta acacattgga taagcctagg cttaaaaaca cagcagtgga
    55321 gcaagttaga catcagaatt gaacgatcgg aactccaaag atttttgaaa gaaaactttg
    55381 agttctaaag gcaaaggaga atgattttat gtcctacaca ttgcaacaag aacatcaaat
    55441 tctaggtctg attaaacagc gtagaaaaca attacaagat gatcgtgcag cgcttagaaa
    55501 atccgatgag ctatcagata gacaagctga actaattgct tctgaacttg aggatttgag
    55561 aatgctagaa ataaaaaata gggagattag attatgaaga agacagacac actttttata
    55621 ggattcattt tggggttatt agtgattgta gcgcaccaaa gtattatcgg aggaagcttg
    55681 ttcgcagcat tgatggtttt aatcaatctg cttgactcaa aagaaaggag caactatggc
    55741 acgagaagaa gcgctaaaaa tcggtaaagt gattgctgat aattggtggg caaatagccg
    55801 ttctattatt ttaagcaagc aacatatcga aaagaaaaaa gcatggcaac aaataaaaaa
    55861 gtgactccgc cgaccaaagc aatgagtcac tcaaaaaatc ataactaagg agattttaac
    55921 atatggaaaa tgaactttcc actctagatc aatatttggc cgatcctgat tggggcaaat
    55981 cgaatttaca actggttgct aacaagtacg aaaaatcaat tgatgaagca attgttgcat
    56041 tgactgatgc agcttccata accgctgatg acagtaaatg gaccaagaga gacttgggca
    56101 ttctaaaacg aggcgttaat tggcttgaag atcagtacag agaagaaaca aaagagaagt
    56161 gatatgagtg tttaaaccac taatagattc atactcggcg gtactaaaga aatttaaagg
    56221 taacgacatt ggtgcaacaa tcaacgaaga agtaaatatc gaacggctga aaacgatgta
    56281 tgacggatat gatggcgatc gagtcattga aattcgcttt attgatccac gacggttcac
    56341 agtacagcaa agaaacttca tctatgcgct cataggcgat attttcatcg atacaggcat
    56401 gccaacggac ttctggaagg aattcttcta cttccgtttt gaaggtgtca cagggcgcga
    56461 aataagcctc aaagacgaat cgaatacaac cgtgagtgat gccaatatct tagcgaatat
    56521 catcctagat ttcatctttg aacatcatat tcctttcaaa gaaggatatg agattttacc
    56581 agcgaatcaa gagtattact tctacaaatg cattacaaaa agagtctgct gcatctgtgg
    56641 caaaacagga gctgatatcg atcactttga caaagcgctg ggaagacgaa agcgcaaaga
    56701 agttgatcat tcagagtaca catttgcagc actctgcaga atccatcaca cagagaaaca
    56761 caaaataggt gtgatcaatt tcaaaaataa atatcaaatc aaagggatca agttaaacca
    56821 ggaaacaatt aaaaagttaa ggataggagg ataaatttgg ctgagataag ttggatcaaa
    56881 cttaaaacta ctatgtttga cgatgaaaaa atacgattaa tccaagctgt tcctgagtcg
    56941 gatgccatca tcgttatatg gattcgatta ctagttttag caggaaagac taacgacgat
    57001 ggtctgatat atatccagag gaacatgcct tataccgaag aaatgcttgc tacattgttt
    57061 ggcaaaaacg taaatacggt tcgcttagcg ctaactacat tggcaaattt caacatgatt
    57121 gatctaagca gtgatggact aattgccatc agtaattggg aaaaacatca aaatatcgag
    57181 ggtatggata aagtaaggct aaaaaatgct gaaagaaacc gtaaatacag ggaaagaaag
    57241 agacaggaac gtctcaaatt ggaaaatgac gttagcgtga cgtcacgtga cggtacagat
    57301 aaagatatag aagaagataa agatatagat aaagaagaaa agaaaggtaa gtattctgac
    57361 gaacacttac gccttgctaa aaagttgcaa agtaatttaa ctgaagattt tccaaaagaa
    57421 atgaacaaag tagatatcga aaaatgggca gacacaatca ggttgatgga agaaagagat
    57481 aaagcatcta tagaagcgat tgagtatgtg atcaattggc tacctacaaa tgaattttgg
    57541 tttggaaata ttagaagtgc taagaaattg agagaaaaat ttgagaagct caaattcgaa
    57601 atcaaagcag acaagaagaa tcataaaaag caaagtcaaa aactacagta cagcaatcct
    57661 agtgaatatg acgacttgcc aatttaaaaa ggagatgcat cacatggaaa gcctagcaaa
    57721 tgctatggag aaactaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
    57781 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
    57841 atgcatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
    57901 atataacgat agcttaaaag cacgagcgtt gagttttttt caaaatggtt cggtattaac
    57961 agataaaact ttgtttaaat gcaaaatgga aaattatcac gtagtggatc aagaaacaaa
    58021 aatcgcttta gaaaaagcaa aaagctatac gaatgaggtt ctgctgaacc atcctgcaca
    58081 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg ccacagcttg
    58141 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagaatt
    58201 attagagcaa ataaagtttt cttataacaa tactgaactg agaaaagaaa ttgaaggatc
    58261 gcttatagcc gatattaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
    58321 aggtagtggg gtatcaaata gtaggcagtt tacaaacaac acgttaaaca cgctcttaga
    58381 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
    58441 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
    58501 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
    58561 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
    58621 tattacgatc tagccttgtg gcaagctgaa gcaaatggct ggaaagtaaa acttcaggaa
    58681 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
    58741 attcccgact tcacatttta taaaaacggc aaacttgtca aagtcgtaga tgtcaaagga
    58801 atgcagacaa aagactttaa gatcaaggca aagttgttct gtcatcaata tcaagcgccg
    58861 ttgatattag ctaaaaaata tcggaatacg ttcaaggaag agcgttttta acgaggtggt
    58921 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aaacattcag cgtgaaattg
    58981 acgatctaga acgaacaaag gcagtgatgg tcaatgaaac ggcgagaaag gcaatcgatt
    59041 tgcacataga gaacttaaga agggaaattc gtagattgga ggaatgagcg tggataagaa
    59101 agcgacaatg aaacgaattg ctgaattaac caagtcagaa tcttggcagg aagacaaaga
    59161 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg actgaaaagc ctaaacggaa
    59221 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtacagctga
    59281 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
    59341 atggattgat tcaaaaggac gacagtttag gtatgtggag gagagataat ggatctcatt
    59401 acacaataca gtgacatcat cctcaagaaa atcatgatga agattcagaa agacaaaaaa
    59461 tcaaaagaac gagctgaatt agttaagtta gaaatggctg aaacaggagc aggagtgcga
    59521 agtagcaggc attggaaagc agcagcaaac attgaatttt attacaacga aattcaaaaa
    59581 gggttcgatc agatgcgtga gctggatcgg caaacaaatt ggagcaagaa acttcatcaa
    59641 gatcgtttca aatttgtaga gaagtataaa gaaatattag aagagtattt gaggaggaca
    59701 gcaaatgata aaaaaactcg ttcaattcag catggattta tatgatatcg aatcaggagc
    59761 aacactatct gtggaatcgg accatctaat cataaatttt ggtggaaagc gccagattat
    59821 tttgtgggta gttgatgatg tactgtttcc agaaattgtt catgatttcg aagaatcaaa
    59881 agcggttgag tttgaaatag tgaaaaaagt aatggaattg attgaaaaat acgaggagga
    59941 cagcgaatga taccgaagtt tagagcgtgg gatagagaaa aagacagaat gaaggcagta
    60001 gctttggttg aatttgctga ggcgtataaa tcaggagaac tcatttcacg tacaaaataa
    60061 aaaaagtcgg attcctccga ctatgaataa tatttccgac ataagtatta tatcataatt
    60121 gggggaatca gaggatggta cttttcgatg taaagaaata tgagacacca agcgcgaaag
    60181 atgttgatat ggaacgcaca aaacataatg tcgctgtgtt tctttcagca tatctatcag
    60241 ctagatgtag agtagggcaa cctcgagaac caaaagtgac agcatcttat tccttggttc
    60301 caccttctac agctgatcat atatttgaag cagaaagaat gctgatcgat aaagaagaag
    60361 cacaggaaga atttgagtat ctgcataaat tgtttgttcg aggctattca gcgatacagc
    60421 atccgcataa gcctgatgtg actgaaaggc gcaagaagat attctatgat cgttatatca
    60481 atggtctgcc catttatgta actgctcaaa ggaataatac tagcgaagaa tcggttaaag
    60541 tagaatcaaa cagaattatc atccaatttg cttcatcgtt agaactggtt gctttcaagt
    60601 agccagtttt tacacttttt ataccctttt attaccaatt tggtttccat tttatacctt
    60661 ttttatacca atcacttacc tattcaacgt tgtattatga tagtgtcgaa agattaggaa
    60721 acaggatcga caaaataaaa tgtaagggag gaaatctccc tcatcgttta attaagcttc
    60781 gataggcagc agcggaaata ttaagaataa ggatgtgaat ttcaactcct tctaaattgt
    60841 tcttattatc tatcatccgt tgctgtctat taatttatgt attggagggt atataaaatg
    60901 aagatcgctt ccgaaaaaat tgatcaacta acaattttag taaaccaaag aatcgaagat
    60961 attatcaaag aattcgaagg tgaaaagatt aagaacataa agcttacttt aatggatcat
    61021 aagttcgaat taaattcgac aaaaaggaag atcgttgagc ttatagaaga agaaatagct
    61081 ctccagatca atgagcaaca attagctaat taatttatgt attggagggg aaatgaatgg
    61141 aagtcgaaca aataaagtat caaggtatgg atcgggaacg gttgattgat attattgagc
    61201 aacaacgttt agaattgatc caaaaagaag ctgtatgtaa agagtacaaa aaacatttgg
    61261 aacaagtaat tgagtatcat tcagtagaaa agtacagatc agtggtgcaa aaaaatagag
    61321 caacgagtcc tgattctcgt caagatgtat cgattagtat caagacgcca gtatttaatt
    61381 ctgttaaatc agaaaagcta gaaagtatct caactgcttt ggtagaaaaa gcagctgaaa
    61441 tgaactccct agcaaatgaa gttagtcgtc ttatttagtc atatcgataa cttgagagtc
    61501 tttggtgatt ccgtttttta tagaattaat ggtttgttca gcggaatatt tagtcaagta
    61561 tgtttcgcta gttgctacta cttcattgtt attggactta ataacgaagt aatattggcc
    61621 attagttgct tctcttataa caaagtacaa atttttcacc acctataatt tatttcagcg
    61681 gaccactcgc tgataattaa aattataagc tatgtataaa atttcacaat atcaatttgt
    61741 cgctgtggcg gaaagggtag acgcaataaa ggatgaatgg gaaaagtcaa tgagtaatta
    61801 tctgaaattt acaagtcccc tgtttagtta tctaagatta tttgataata ttatttctga
    61861 ggtgatagaa aatggtgggc gtagttaaat ttcttgggtt tacatttgat ggatatggta
    61921 cgttaggcga atgggtaggt attattttga tagtgattac aattcaacaa acatcaaagt
    61981 actactggta ttctaatagt tctaaaattg tatcaagaat aactgaagga ataaccaaaa
    62041 aagatattac tgtcgtcggt aaatatgggt ttactataac tttattaaat gaagggaaat
    62101 ctgaaaccgc aataaagttt tgcgggatta taggtaaata taacattttc caacgtttta
    62161 tcagaaagca ttggtacaaa tatatttaca aataccctgt agttaaaaag cttattgaaa
    62221 aatatgtgga ccacgaaata tcttatctca atccaacaga ccttattaat caagaattcc
    62281 agaatattgg atacttagaa aaaggtctaa aaataccaat tgtaaatgaa agtttagtta
    62341 ctgcaatggt agctaacata aagaacagca agaaattaga aaagagatat aaaaaatgta
    62401 aaaaattgaa attttccttt ttattcttag aatataatgg caaagaatat tatcaagata
    62461 tttatgtttt gcgaggctcg aacttatata aagacattaa aaataagatt tcagtatgag
    62521 attttttaaa attcgtagat cactctttga gtggtctttt tattttgcgt aaaggaggca
    62581 cgacgatgta tagaccacaa tacttagaac agaagcatga agtaatcact gtgcaaaacg
    62641 gtaacggtga gatagtacga aagtatagaa gaccaataaa gagcgataca tataaacgga
    62701 aggaaagcaa tgaagttatt ccattgtatg gcaaaagaat agctaagtat taaataagat
    62761 tgcgaaagga gatgggacat gaccgaggaa ttctatagat ggttattaca gttgataaga
    62821 gaagatcgtt tggttaagtt ctatcagtct cctaaatggc gcaggcttag agagaaagcg
    62881 atgaaacgag atcactatga atgccaagag tgtagaagac taggtaagta tcatagagta
    62941 gagaacgttc atcatataaa ggaagtcaag gatagacctg acttagcttt agatttagat
    63001 aatcttattt gtttatgtgt tgaacatcat aatgaagttc atggcagata tcttacagcg
    63061 ttagataaac aagagaagaa gatagaaagc ttcgctaact tcgatgcaag tgaaaggtgg
    63121 taagtgcatg atcatcaatg acaatggcag agagtatgat acagaaaaga ttgaagagta
    63181 ttcatcttat actcagggat taattaaacg tttgatatac gttcgctatg taggtattag
    63241 ggatctgtta tcagataact gttgtagtaa atacaaagtg aatcaagtaa gagaagcgtt
    63301 gaataaagat aataacgtcg aaagaataaa aaatgttttt ggatatagta ttgaagagat
    63361 taattattac attgacttcg ctgaagcttt cattccgatg gtgagataac ccccccttaa
    63421 aataaatcgc aaattttttg ggggtgatga aacggagggg gctgtcagga aaagagattt
    63481 tttcgaactt tatcatgaaa ggagggctaa aatgtttaaa aacgaattgt ctcaaaatcg
    63541 ctacagagaa aaattacgcc gctctttaat aagccaattg gaaagtcaga aaacaaatat
    63601 tgagccattc ttagataatg ttgatcgtta tatcagttta tgggaaacgg cgatatcact
    63661 ggaagaagat atatccgaga acggcattag actggagaat ggtaaaaaga atgaatcagt
    63721 agcgttgctt gtttctgtca acaaacaaat gggattgatg ttggataaac ttgccattac
    63781 tcctgaattg gtaggtgaag caaatgaatc aattcctgag ttataagcat attgaaaatt
    63841 ggttcaaagc tatagaagaa ggcactatca aggtatgcaa agagcaatta ttgctaaaaa
    63901 attatctaga agaaagagtc tttactagag aagatattta cttcgataag cagatggtag
    63961 aggattcaat caatatacca gcacaatact ttccattcga attaattccg tgggaaaaat
    64021 ttctacaatg ttttatttat ggtgttcgat ggaaaaaaga taaaacacta gtgttcaata
    64081 gatatctttc attaatggga cgtggtaatg gtaaaactgg ttttgcttct tggaacaact
    64141 tctttctact aaccgctaaa cacggtatta aaaattatga tattgatatc tatgccaata
    64201 atgaaagcca agcaaagact agttttgatg atgtatttaa agtaattaaa gatcatcctg
    64261 atttagataa aaaagtattt aaagctacga aggaagttat tcaaaatatc gctacaaaca
    64321 gcaaacttcg ttataacacg gcaaatgcta gaacaaaaga tgggaagcga ccaggtgcaa
    64381 accgctttga tgaaattcac gaaaatgaag attattcaat gataaatgtg gctacttctg
    64441 gtggtggtaa aattcgagat tatagagaat tttatgatac aactaatggt catgttcgtg
    64501 gtggtccgct tgatgacatt atagaagaat caaaaatgat tctttctgga gaacttggaa
    64561 ttgacaagga tggagcagaa ttttctagtt tgtttccatt tatttgtcgc ttggataacg
    64621 ataatgaagt tgatgatccc gacatgtggg aaaaagcttg tccaactatt aattacaatg
    64681 cagatttaaa acggaaaatg tttcaagaat actctcaaat gcaacgtaat gctggtttaa
    64741 gacttacgtt catgaccaaa cgaatgaaca gacctatgga agatacacga tttgctgttg
    64801 cttcatatga tgatgttctg catacgaaag aaaaagaatt tcctgaaaaa atggatgaag
    64861 tgataggaat agtcgatttt gctgatagac gagattttgc cagcgttggg ttgctaggaa
    64921 aatacgataa agatgtttat tttacacaac atacttttat ccacgaatca gcccttcgat
    64981 tacaaaacat caaacgagag gttatagata tttctataga tcaaggaaaa tcacagatcg
    65041 ttcatggaaa aaatatagaa gctgattata ttgtaggttg gtttcttgaa atgagtaata
    65101 aatattatat taaaaaaatc gctatggata tgtaccgtgc aaaaatattg aagcccgctt
    65161 tagaagaagc aggttttact gtggaaattg ttcgaagcgg atctgttaca catggtatgt
    65221 taaaagatct ggttgatgac ctttttatta atcaacgttt attttttggt gacgatgcga
    65281 ttatgcgttg gtattgcatg aatgtatatg aagagcatat ttctaatgga aatatacgct
    65341 atgaaaaaat agaacctgaa actagaaaaa cggatggctt tttttcattc cttcatggtt
    65401 tgaatttttt agatgatatt tatgattctg ctcctgtaac agtcacaaat agctcagtag
    65461 aaaatacagg aactggattt actcctctag tattctaact tgaaaggagg tgagaaagtg
    65521 gggatttttc aaaaggcggt aggatacttc acaaaaaaag caacggttcc tttagaagaa
    65581 tacttttgta aattgcaagt tgattttgtg tatcgaaaat ttgcgattga aacttgtatt
    65641 gatatgattg caaatgcgat gagtaaagcg gaattcaagt catatgaaga tggaaaaaat
    65701 aaaaagaatg atctttacta taggctgaat gtagctccta ataagaaaaa taatgcaaca
    65761 gaatttagaa aaaaactgat caggagatta atattctaca atgaagtatt gatcgtttct
    65821 ccgtctaata attctagcga aatatttatt gcggatagtt gggatgtcac agaatatgca
    65881 ttgaaagatg atgtgttttc tcaagtgcaa attaacaaca tagtccttga tagagaattt
    65941 ctagaaagtg atgttatcta tataaaatac gcagatcaac aaattaggca actagtcgat
    66001 gcgtattatc aagcgtatgg gaaactcatt tctagtgcca tgaatgttta caagcgctct
    66061 aacgctcgta gatacgtact gaaagggaat ttattccgac cgcaagacaa tacaacacaa
    66121 gatcaaatca ataaaatgat gacatcacaa tttaaggctt ttatggaagc tgataatgca
    66181 ggtgcggtat ttcaattaca aaatgagtac acattagaag atttcagcgg aaacttccaa
    66241 agcaattcaa gagatataaa aaacttaata gacgacatct ttgagatgac agcagcagcg
    66301 tttcacgttc cgaaaaacct actaaaggga gacatgagtg ggttatcgga tcaagtggac
    66361 gcttttttaa tgttcgaaat cataccgatt gctgaactta ttcaggatgc gtttaacgct
    66421 agtctctatg aagtagaaga atacttgtca gggaattttg tacgtgtgga tacaactatg
    66481 atcaagatta ctagcttcaa agatttggtt gacgctattg atgtaggcat tagaaatgga
    66541 gtatttacaa tcaacgaagg aagagagcgc gttggaaatg atcgctctga taaggcgatg
    66601 gcagatgaaa tatttataac taaaaacaat caacaagtat cgaaaggagg tgaggcgaat
    66661 gacgacaatg aaaacatttc tagcagtaaa gaatgaaggc gcagtaccgc aaatttttat
    66721 tcagggattt attggttcta gttggttctt tgaagggaat actgacaagg gaatcaaaaa
    66781 tattttggat agtctaggtg atcaagaaga aattgaagta gtaattaatt caaacggtgg
    66841 agacgtattt caagggattg ctattgggaa cttacttaag tcaaataaag caaaagttaa
    66901 cgttgtgatt aacggcttag ccgctagtgc tgcttcaatt atcgcaatgg ctggcgatac
    66961 tataaaaatc tacaacaatg cacaattgat gattcaccgc gcttccacat acggagaagg
    67021 aaatgtcgat gacttccgta cgattgctga ccaactggaa tcaattgata aatcggtaaa
    67081 ggcttcatat aaaacacgat tcaatggcac agatgaagca ttgcaagaac ttcttgaaaa
    67141 agaatcgttt atggatgcag aaacagcttt gagttatgga ttggtcgatg aaattatcga
    67201 tgcagaaaat agctcaggta ctgaagctaa aaaagaacaa agcgttgaag aaattttgaa
    67261 tgacgttaaa gaaaaaagag cagaaaaaat tgctgcattt acagcagcat taaataaaac
    67321 atttggacaa ggagatgtaa aataatgaca gttaaaaatt taaaaggtgt aacagctgca
    67381 agcgaccaat tgatgaaagc ttttaaagat ggtaacgaag aatcttttag cgcagctatg
    67441 gtaagcttat ctaaggaaat tcaggataaa attttagaag aagcaacagc aaaaaatcaa
    67501 gatcaattag tattaatgaa ccgtggtcag cgtgtattaa ctacacaaga aacaaaattc
    67561 tataacgaag tagtgaaaaa cgaaggtttt gcaggggtcg aagaattagt gccagctact
    67621 gtatttgaac gtgtatttga agatttagaa caatctcatc cactattgca aaaaattact
    67681 tttgttaaca caactggtgt aacagaatgg attgtgtcac gtggagtcaa tccagcatgg
    67741 tggggtaaac tttgcgaagc tgttaaaaaa gttttagata atggctttga tgtaattaac
    67801 atgaagcagt tcaagctatc aggttatatt cctgtatgta aggcaatgct tgatttaggt
    67861 ccagtatggt tagatcgtta tgtccgtact gttttagtag aatcattgag aattgcatta
    67921 gaacaagcaa ttgttgatgg tactggtaaa gatatgccag tcggaatgat gcgtgacatg
    67981 agcaaacaaa ctagcggaga atatgctgaa aaaaaagcag aacctattac agctttagat
    68041 gctgtaacta tgggcggttt gatggcgcga ctatcaaaat tcaatatcga aggtgtgaat
    68101 gatccgattt atcgtaatgt gaatccttct gatgtggtcc taattgtgaa tccaactgat
    68161 tactggtcta aagtattccc agctaagact gtactaactg ctaatggaga atacgtacaa
    68221 gtattgccag taccagtttc agatttgcag tcaacggctg tgccagaagg aaaagcagtt
    68281 attggggtag cctcagatta cttcatgggt gtaggatcta cactaaaaat tgaagcttca
    68341 gatgaatacc attttgttga agacgaacgc atttatctag ctaaacaata tgcaaacggt
    68401 caacctaaac gtaacgatag tttcattgta ttagatatta gcgctttggg aactactact
    68461 acaactacaa aaccaacaac cacaacaact acaacacaag cgtaggtgat cagaatgaag
    68521 tatattcttt gtcagccggc aatcaatcgg tttaaatggg agcttgaagt ttgtttaact
    68581 aatctgaaga aactaggaat caaagatatc gtattgcttt tcagcagaca cgatgatcag
    68641 attcctattt tttttgagaa ggaatatggt gttgaagttc atgtgtacga tgatctgcgg
    68701 gacgacaaag agtatattcc ttcgattaaa ccatatttat ggtggaaata tttagaagaa
    68761 gatcattcgc gtgaggacga ccgatatttc tatatcgatt cggatgtcat tttcaataaa
    68821 agaattaatt tgcgcaaatt gccttctaaa gatgatgttt ggtattgtag cgactgctgt
    68881 agttatctaa gtcttgatta tattagaagc tgtgaaaacg gagaaaatat tctaaaagat
    68941 atggcaaaca ttgtaaatgt tacagtagaa tctttggaaa ctataaacac taattcagga
    69001 ggcgcacagt gggttattaa ccgccctaaa gcgaattatt ggaaaaaggt ttatctggat
    69061 tctaatcggc tatatcgcta ccttagaggg caaaaaacaa atatacaaat ctggacagcc
    69121 gagatgtggg cacagctttg gaacatgatg tatttcaata ttggtcctaa agttcacgag
    69181 gaattagact tttgttttgc tactgatcca atagaaaaag ttaaagaagt aaaaatctta
    69241 cacaatgctg gagtaacaac aaacgatgaa gatttatttt tcaaagggag atacgtgact
    69301 tctacgcctt ttgatgaaga tttatcattt gtaaacaaga aaaaatgctc ttacgcatat
    69361 gttaaagcaa ttaaggcggt ggttagatga cgcctgaaca agtgactgaa gaattgctaa
    69421 tagctgtgaa ggataatatt tacgttacct ggaacgaaga agatgagtca attaaaaaga
    69481 tgatagctaa aaatgctgtt tatcttcaaa gtaaagtgag tacaacactt tctttttctc
    69541 ctgaaagctt agaatacgga ttgctaatcg aaagatgtag atacgactgg aatcgtgctt
    69601 tagatgagtt tgaacaaaat ttcgctagtg agttattagg tttcattcaa cattatgcgc
    69661 tacaagaata tattgcaggt gatgtgaatg gcgaataatc gtagactcga agaaacattc
    69721 aacgatggtt ggttaaagat tttgacgcaa actaccaaaa gaaatgaact aggaaaaaag
    69781 attggtgtag aagatacaga aatcacttct ttaaaattta gaaatctttc catgagagat
    69841 agtgatataa cagctatgga tgcgatggga tcgaaattaa ctaagaaagt aaagactcca
    69901 tttcatccaa tcgccaagaa atttaataaa gatcaatatt ttatcgtaat cgatagtatg
    69961 cgttacaacg ttatctatgc cgattacgat aatttttata tctattttta tcttgaaagt
    70021 gtgggtgaat atggtgattg ataattctaa agaaaaagaa cgtttaaata agcaaatttc
    70081 tgctatcaaa acttccttag aagagcattt tggcctcaaa ctctttcaag actccgtagg
    70141 cgaggatgag ctacctgatg attttaatta cttcattctc gaaacaggag aaatagaaat
    70201 gatcactgag ccaaaatata gcgtgggtca aaatctatat ctaactttct attcagaaaa
    70261 tagagaagat ttaacaggag attcactaga tattatttca ttgattcaaa atcgttcgat
    70321 tcgttttcag agaatggatc ccaatcattt aaaactagag aaccaagatc gctatatcga
    70381 tcaattggta tttacgttta gacgattatt gaagagtgat tgtcatggct aaaaatagtt
    70441 gggagctaaa aataaatgga catgatgaac ttcttgtgcg gatggaacgc tattcaagcg
    70501 agagcgaacg actgattaac gaagcattga aatcaaaagg ttcggctatt gcagtggata
    70561 ggattacaga aaaaattcct gtttctgaag cagatttaag aagagggcac caacacgcaa
    70621 aaaatagtcg tccacttaag actcaataca ttaatttggg tttcatcatt agacctacaa
    70681 gaaaatttga gtatttaaaa tatcctgatt tggggatagg tacttctaaa agaaatcagc
    70741 cggacgaatt tatgagaaga ggattaggtc ttgcacttga tccaattaca gaacttctga
    70801 ttcgtcaatt cgataaatta aataaatagg aggaacaaca atggctaaaa caacaacagt
    70861 aacaactttt gacaatataa gtattaaaag aatttctttt aattttaaga atgcaacaaa
    70921 tgcaatttca actgattgta atggacaatt agatggcgaa acagaaatgc agagaattgt
    70981 aaaaaaatgc ggttcaacag aagtaaaatc gaaatctaaa ccaatcaata tgacggtaac
    71041 aattactgca catgtaccga tggaagttta tcgacgcttc aatgggttga aacaagatga
    71101 acgtattaaa tcgggaattt actcttatgg tcctgattcc gtaggggaag atttctctct
    71161 tgctgcagag atcgtggatg atttcgaaga aaaaaataag ctgattggta tgttagcatg
    71221 cacttcgaat acaggattaa cattctctat tgaaaatggt gcggatgaag tagctgcgtt
    71281 agaactagaa acaaaagtta tgcaagatga atttggtaaa ttttatcatg aagcaattgt
    71341 tgcagaactt gaagaagact taacagatca atggatgacg aatctatctg ctgatgtgat
    71401 taaaaaaact tcagttgtga caactacggc cactcaatca cagtaaaaaa aacggaggta
    71461 gcgaaatgaa cgaagattac tcaaaaattg aactaaacga tggaacaatt ttgaatttag
    71521 aacctaaact gaatatcaag aaattattga tgatcaatag agattttaac acagacgagt
    71581 ttgcaaaaat gactgtggga aaaggatcca tggatatttc tgttattcaa ggtgcaaagg
    71641 ctgtgtatat tgcttaccgc caagcgaaca tgactgatta tatttcattc gatgaattta
    71701 tcgataaatg ggattttgat atggctactg ccagctatat ttatcaattg atgatgttca
    71761 aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc aaataaggaa aaaaagcttc
    71821 aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc gatgtctatt cgatgttgac
    71881 cgacgttttt tctatgcctt cagatttggt tttaagcgat atctgtttag atgacatttt
    71941 gcaaatggct tacaacaaga gtgcttatga aggatggaaa aactatgcaa taaaccaatc
    72001 ccagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa gagaacagaa gcagaagtaa
    72061 cattcatagc taacgatgac ggattgaaat ctacgttaaa agaaatcagt gctgaattaa
    72121 ctaaaaatag agcagaatta aaactagaac aagctcaatt acaacagact ggttctgaat
    72181 cagacaagtt aggaagtaaa ttatcttctt tagaaaagca gtatgaatta caaagtcaaa
    72241 aagttgaagt aactagccaa cgtttagcca atgccaaaaa atattatgga gaaaattcca
    72301 ccgaagttca gaaacttgag agagaactga ttaaccaaca aacagcgcaa caacgtttgt
    72361 caaacgaaat tgataaaacg agtaatgcac tagctcaagc aaaaggcgaa atacagacgt
    72421 acgagtctac aatgcaacag ttggatagtg aacaaaaaaa tgttcaagct agtgcttctc
    72481 tgattgaatc cgaatacaaa aaatggcaag caactgctgg tcaatcagct tctgaatccg
    72541 agaaattagc gaaagcccaa gaatatgttt ctcaacaatc tgaaaatgca gagaaaacga
    72601 tagatatcct gagacgacag ttagaagcta cacagtctga gtttggcgct acatccacag
    72661 aagcaatgca gatggaggcg aagcttaatg atgctgaacg tgaatttgaa gagttaggac
    72721 aagctgctaa aaatgtagat acaactaact tggacgatat cggaagcaaa atagatatga
    72781 ataatctaat ggaagcttct gacgctttaa gcgacattgg cgataagctt acagaattag
    72841 ggaaacaagc agtggactct gctaatagtg taggtagttc ccagagtaaa atacaagcta
    72901 attttggttt gactaaacaa gaggctgaag aattaacgaa tgtagccaga gacatttatt
    72961 ataaaggttt tggagaatcg ttagatcagt ccacagatgc attgattttg gtaaagcgta
    73021 atttaggcga tttaaataat caagatttac aaaatatcac ggaacaagct atggtcctag
    73081 aaaacaccat gggcgctgat atggatgaaa cgttacgtgg tgtaaatggc ttaatggtca
    73141 atttcggctt gagcgctcaa gatgcaatgg atttaatggt ttcgggtact caaaacggtt
    73201 tagataaaac gcacgaatta ggcgacaata tggcagaata tagccaatta tggagtcaaa
    73261 tgggatattc agctgatgaa acgttcggaa tgcttcaaaa tggtttagat gcgggtgctt
    73321 ataaccttga taaagtcaat gacttagtta aggaaatggg aatatcgtta acagatggtc
    73381 gatttgagca aaacatggat atgtttagtg aaagtactag aaaagctttt gaagagtgga
    73441 aaaatggcgg aggaacacaa aaagacgtta ttaattccat gattcaagat tttagcaata
    73501 tggatggtca atacgaccaa ttaaataaag cttctacaat ttggtctgca cttggcgaag
    73561 ataacgcgat gaaagttgtc caatctttga ctgatgttaa ccatacattt gatgatgtta
    73621 gtggatctgc acaaaaaatg aatgaagatt ctactactcc gttacaagaa ttaaacggaa
    73681 aaatagctga attaaaggat tcattagctc ctataggcaa cacaatcata gatgcactcg
    73741 aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt taataatctt ccacaaccag
    73801 tacaggatta tgccgtagcg attggcggat tgactgctgc atttacttta ttaatgccaa
    73861 taatagttgg cttcatggct ctaggtggtc ctactacatt aataatagga gcagttatta
    73921 ctgttattgc tggagttata gcaattataa aaaactgggg cgcaattact gactggttta
    73981 agggaatatg gagtaaattc actgattggt tgggtggtac ttgggaaagt ataaaagaag
    74041 gtgcctcatc agtttgggat ggagttaaag aaacctggtc tggatttgta gattgggttc
    74101 aagatatttg gcaaggagtt tctgattggt ttggagagct atggagcgga ttagttgaag
    74161 gagcttccaa catctggcaa ggagtccaag agacttggca agcattcgtt gattgggttt
    74221 caaatatttg gaacggagtc aaagaagtat ggtcgattat ttgggcagac attgtaggaa
    74281 ttgttcaaat accatggacc ttaataacgt cattgattca agccggtatt aatattatcg
    74341 tgggtatttt tgatgtagct ggacagttat taggcgcagc ttggcaagct gtttggacac
    74401 ctatttctga tttccttaaa aacacttggg atactatgac acaatgggta agcatcgctt
    74461 ggaatggaat tgtaactaca ttccatacta tatttgatcc agtagtggca tggtggaatg
    74521 gtatatggac agctattagt actacggctt caaatatttg gaattcaatt agtgcaacag
    74581 cttctagtat ttggaacagt atcaagaata caatcactag cttggtacaa gcagctgcta
    74641 cagtaattca aaatatttgg tcaactgtat ctagttggtt aggtggaatt tggaattcaa
    74701 tcagctctac agcatcaaat atctggaata gcgtgactag tagtataagc aatgctataa
    74761 acgcagctaa aagtgccatt caaagtgttt ggaatagtat atcttcgtgg atcagcggaa
    74821 tttggaacgg tatcaaaaac actgctttga atctttggaa tggaattaca agcactatta
    74881 gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg ttggtccaat ctaacaggta
    74941 ttgtttccga catattcaat aatgttaaaa gtacaattgc taacatttgg gaaggtatca
    75001 aaaagactgt tagcgctcca attgattgga tcagagataa aatcagtagt atctttgata
    75061 atttgaatat ttctatacca catattccgt taccacattt taaattgagc ggagaattca
    75121 atccattgaa ggggaaaatc ccaacgttgg gtgttgattg gtatgcgaaa ggtagtgtgt
    75181 ttaattctcc gaatattatc ggtgtcggcg aagcaggacc tgaagcagtt ttacctttga
    75241 aaagatctgt gctgcaagaa attggtgatc gtatcttgag tagcacatca gtttcatcta
    75301 gggcacaaac gattcaacct gtgaataact acgaattcaa tttcacaatt gatggtaacg
    75361 cagatgaggt tactatgaag caaacaactc aacaaatcat tgatagcatt acaaaagttc
    75421 aaaatgataa tgcttcggca tggcgttaaa caggagagta tttctcctgt ttttttagta
    75481 ttaaaaagga tgtgaaaaaa tgactgattg tatacattct ataatcgatg gatttcctga
    75541 ttatttgcat aaattggctt tagctgaaag accaaccata ccttctccaa aaagacagag
    75601 agttgaaact tctgttttag gaaggttagg tggcttagta caagattact cgtttgaaga
    75661 catgtcgttt acattgcact ataactattt agaggatgtg gaagaccatc aagcgttcaa
    75721 gcaatcgttt tatatcatgc gtcattggtt aaattatgca aagaaattag aattctctga
    75781 tgatcccaac gtctattatg ttatccagac tatcgatatt ggggatgcag aaaacgatat
    75841 tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa ccattcgcaa gagttcaaga
    75901 agatgtacct ataaccgtag ataaaccaca gtcatttaac ttgctgaata atagtttaga
    75961 agaaagtttt ccaaagatta tcatcactcc ttcagctact tcatgccagt tcatcttaaa
    76021 tgattatgtg tttagttttg aaggcttagt agtaggaact gacgtagtca ttgatagtga
    76081 tttgatgctt tgctacgaag agcaatcgga cggagatatt ttagatcggt ccaacaaaat
    76141 gaagaccatg caatatccga cattgcaagt ggatattaat tattttaatt gtactggttt
    76201 gagcaaaata caaatttatc gtaatgggtt aaggtaggtg aaatagatga tcgataattt
    76261 aataactatt tacgataaaa acgacgcgaa taatttagct gaacatttat atgatacgca
    76321 aggtttaggc gctttgtcag actggttaac agctactgtt agcaataaat taaacggagc
    76381 cgagatattt cagggtactt atccaataag cggaactaat gcagatttga ttgtagaagg
    76441 acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa cagcgtctac ggatttatta
    76501 tgcaaagact tctgtaatag gcaatacgat agaagtaaaa gctgaaccta ttttcaatga
    76561 tataagaaaa tcggtgttga ataaatatga cagtggaaca gaaaagatca ctgctagtca
    76621 ggcatggcaa aacgcaaaaa ctttagcgaa accagttatt ccttcacagt tttctttctc
    76681 gtcattagta gatacgcttg ctaatgtgaa gatagaaaag gcgaattttt tagaattctt
    76741 tggtggaaaa gagggatcta ttctagatcg atttcatggg gaatttctaa aagataataa
    76801 cacattacgt catgaaaaaa ggctaggcac ggatcataaa atcaaagcga tttatactaa
    76861 aaacttaact ggtcttgact tagagattga tgctcaaagt gttttagttg gagtttatcc
    76921 attcattagc agctcttcag aaggagaaga cgagatcact ctaccagaag aagttatttt
    76981 cacggattac gtggatgatt atcctgctgg atatgtttct tttgttgatt ttaaagacaa
    77041 agcgactgat gtagccacat taagggaagc tgctaaagac tggttgaaaa caaacataga
    77101 taaacaaaaa ccacaagtga gtggttcgat tgaattagta ccattgaggc atcaaagagg
    77161 ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt gacggagtag atgtgtatca
    77221 tccacagtta aaagtgaata tgtcagcgag aattgtggaa tatacgttta atgttctaac
    77281 caattcatac gataaattag ttgtaggaaa cgtcaaaaca aacttcttag aaaacacaga
    77341 gaataatgtc agcaatttga ttaatgatgc cattgatcaa ttgaaaaacg gtggcgaaat
    77401 cagtgattta ctcaatgata ttgtagatca tcaaactgat atgattactg gtaaaatggt
    77461 ggttatgttt tattagatcc taaagaagcg cctagtcgta ttttgattat ggacacacca
    77521 gataagaata ccgcaaggaa tgttttacaa atcaacaatg ctggtattgg tttttctaaa
    77581 actggcatta atggaacata tgaaacggca tggacgttag atggcggatt caatgcctcg
    77641 tttattacgg ctggtgaaat agtaggaatt actattagag gtactacatt aattagtgat
    77701 ggtgctgatt atagaacaag tattgctaat ggcaaaatga cttggtactc aaaaaaagtt
    77761 aacaaagata ttatggagct agaagcacgt gattatgtaa gtgctgatgc cggtattgta
    77821 tcatacacca tgaaaactgg tggtggtttc atgattagaa atccacaggg taacttggtt
    77881 tttagtacgt gggataatgg taataacaga ccgtttctat cttttggtgc gcccaatttc
    77941 aggtatagca atgctagtta tgtaacttct ggcgacggta gttctttaag cattaatggt
    78001 agtgcgggta actcatggga atttaaggta gctggtagga ctatgaaatt tactagtgat
    78061 ggtatgctaa cgttaccagg ttgttttttt ggttcatggg aagatgggaa acttgctagg
    78121 tttgaacaat caacggtaca agtatataaa gattttactg ttagaggtac taaaaactca
    78181 actgtaccaa cagaacatta tggacaacga ctattgaacg cttatgaaac tccagaatat
    78241 tatttcgctg attatgggga agccgttaca ggtgacgatg gtaaagttcg tgttgatatt
    78301 gaccccatgt ttgctgaaac agtaaatcta agtcggtata tgacacatgt gacacctaca
    78361 gaactagttt tgtgtgctgt tactcatgaa gatattgacc atttcatcat tgaaactagt
    78421 aagccaaacg tattagttag atggaattta gtggcacacc gtctagggta tgaagatatt
    78481 agattaaaag aggatacagc atatgatagc acagtgcttg accaaaaacg tttttaaaac
    78541 gaagacaagg aggtatataa atggctagca gtttatataa tttggcttta gatttcagca
    78601 aagaattaaa ctacaccaaa gctattatgg ctcgtcaagg tgataaaggg attacggtga
    78661 cggttaaacc gtttctaaat ggcttgcaga tggatacgag tggcggaaca tttactttaa
    78721 aaggaacaac accatctaac cgttacgtag ataatgttgc aactagtgta actagtgaag
    78781 aagtcacgtt ttctcttgat ggcacattta tgagtgaagc aggatattat aaacactgct
    78841 acgtagaata tagaaaagac aatcaaattt taacaacgca agatatcatt tttttctcac
    78901 taggagtgtc tgacatttcg caaggccaag ccgatgaata tgtttcgcaa ttagaagagt
    78961 tgattcgaaa gtacaacgaa acttttgatg cttttatggc tgaaatcaaa ggtagagtgg
    79021 atagcttaaa tcaacagatt actgatttaa ctggtcaagc taaaacgcta caagacaagt
    79081 tagatgctct gaaagaagaa atttctaagt taggtaactt acaagtgatg tacagtaaca
    79141 gcatcgactt cgggggatat gattatagcg gtaagccgaa tttaatgtcc aaactaaaat
    79201 cgagcgattt taacgttggt taccacgggt cactaacttt ggataacgaa aagttacatt
    79261 ttacttctga tggtacagga agcattatta tgtttacgcg tattaataca cctcagctta
    79321 ctagtgggaa aacctatact ctgagtgcga aagttcgatt tgatgaagga actacaggag
    79381 ctattgataa attacgtttg gtgtatcgta catcaccagg agaaaagata ttattggaag
    79441 caaatagtac aaatattaca acagatgatg tagggaaaga aataacaatc aaaggtacag
    79501 ctaacgttaa ttatcaaatc acaaatttag atcgatttta tatgagtatt agctttgttg
    79561 acagggataa aataaatggc ggatttaagt tgtacgacat caaaatcgaa gaaggctcaa
    79621 cagccacacc gtaccagcct aatctacttg atgcaccgta ttatttgagt aaggtggctt
    79681 tgggtgagaa tctaattaaa ccagaatcac aacaaccagt tactaatagt aactatctta
    79741 ttaacaccta taacattaaa ccaatggtaa aaggtaagaa gtataccatc acacttgaag
    79801 gaactaagcc agcaacacag gtttttagac catttttcac acgagcaaca ggagatgcat
    79861 gggaggttgg tgacttacaa ccagtagaag gcttaactaa tgtgtggtct aaaacattta
    79921 cagcagcaga tgactcacac cctactaccc cacaagtgca gatttatcaa gtaccaagca
    79981 caagtgtagg acaatgtaca attaagtggt taaaactaga ggaaggcaat actcgaaccc
    80041 cgaatattag tgagtataaa tatcgtggta ctggtatgcg tgattcaaac aatccaaaag
    80101 attatgtttg ggatctagca ccagaatatg tcgaagataa tttggccaca gatattaaaa
    80161 tttctgaaat tactggtaaa gcaaacaatt ataccgatgg gaaagtatcg gagattaatt
    80221 cgcagttgac tgcttcaatt aatgaagtag acaccacagc taaggatgct caaacaaaag
    80281 cgaatgctaa tgcgactgct atagatgaat tagacaataa gatcgatgaa cgcattaatg
    80341 atacagctac taccacatta acagttacaa acgggaatac cggatcagca aagctttatc
    80401 gtgaaggaaa aacagtttct atatattttg tggctttaaa cggaaaacgc agtggtggaa
    80461 atgattcaac gatactaaca attccagaag gctatcggcc accaattagt tttgagcaac
    80521 tggttggctc gatagaccgt tctactttga acagtgctca gttatctatt ggtgcagatg
    80581 gagccattaa atggcgaaga aactcaagtt atggatcgga ttataccttt gcaattactt
    80641 acacgattta gaaaagcgtg aatcgatatg aaggcagcat atagaccaat tgaaccttac
    80701 ggattcgagc aaatcattgt gaatgatgaa gaacatttac cggaagaatg cacagaagtc
    80761 gaaccaccga ttccaaattg gaaaccgaaa ttcaattact gggagggaaa taaatgaaaa
    80821 acatttggaa atatggacgt actggcggag agtacgcagg aaaagtattg gacgacatgc
    80881 ttgtatccgt tccttacaca gatcagccac cgcttgaagg aattcgtgct gatggcgaac
    80941 cgttaacaat cgctgatcaa atgtttgatc ctaaattgaa tcaatggatt attttagcga
    81001 acgcactaga tcacaacgat ttaaacaatc tcaaagcaat gtatgagtcg ttagaaaatg
    81061 agaacggcga tttaaaacag atcaatgcca aactcatgct aagcgatgta gcaattaaac
    81121 aggaaaatac tgcattgaaa gaaaaagcgg atagtttagc acaaatcaat tcaaaaatga
    81181 tgcttgcttc gttacaaaat agcaaagaca tttcagaaat taaagagcaa ctaaatccag
    81241 cttcaaaggg aggtgagtag tatgtttagt tttagcgatg tgaaaatgat gtatgattgg
    81301 ggctgtttca ctaacgaaca agtaatggtt ttcgttccgt tgtgcattac tgaagaaaaa
    81361 gcagataaaa tcattagcaa agaagagagc gcatcttaat tgatgtgctt tttattttga
    81421 ttcaaggagt tgtcacatga ttaatttagg ggaatgggga gcgatagcag gatcaataac
    81481 cgctatcgtt tctttgattt tattagtaat aaaaccaatt actgcatctt tctcgaagat
    81541 tactgagact ctttcaaaag taagtcacaa tttagatttg ctgactaaag atttagaatc
    81601 gagcaaatca gatcgattga tgattcatga agaactaaag aaacacgatg aaagattaga
    81661 tacacatgca gaaaaattgg tagaacacac gcaacaaatt aaaactttat ttagggagag
    81721 aagaaaatga ataataaaac gttcgaagta ctaaaatggt tcgcactggt aattattccc
    81781 gcactagcta ctttcgtggg gttagttggt aaagcgctca attggcagta cacagatatc
    81841 tgtgttgtca tcattactgg ttttggcgcg tttttaggga gtgtgttggg tgtatcaaat
    81901 cgaacctaca aaatgttctc ggctgaaagc gaagaaggag gaaacaaatg aaaaagaaaa
    81961 ttactattac tgcgatgagc ctgttaacgg ctcttttttt attacccatt aatacgtttg
    82021 cttatactat taatgacgag tataatttag cgccgaatca aggagactcc agattagcaa
    82081 ttcctaacaa aattattttg cacgaaactg gaatagatgc accagcaaga aacgtagccg
    82141 ccaacatgaa aaataattat aacggaagca attcttatac tacagatgtt attggtgacg
    82201 gtgggattgt ttaccgtgtg ggtgagcaag gatatgtttc gtggggagct ggtaacgcta
    82261 atccttatgc gcctgtacag attgaattac agcgcacata tgataaagca ttgtttgaaa
    82321 aaaactatcg agcttatatt gaatatacaa gagatagtgc aaaaaaatat ggaattccat
    82381 tgactcttga tcaaggaact tctttattta caaaaggaat catttctcat ttgtgggtga
    82441 caaattatgt ttgggggaac cacacagatc catatggtta cttatcgcaa atgggagtta
    82501 gcaaagaaaa gcttgcttat gatttagctc atggatttac cgatgaaaat ccaacaactt
    82561 ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct acgctgacag
    82621 atggaacaaa tcacgcccac attgatcagt ttggggaaat cgaaaacgca aacttgcatg
    82681 tggctggatg gcacattgct aactataaat acgagtatat tttcattatg gactacaata
    82741 ctgggaaaga attagctcga gtaagagctg atggaattta tagaccagat gtaaaccaag
    82801 cttataatac ttctggaaat gttggttatc atgtatcttt caatatgcgt aattttccta
    82861 gtaagaaagt ctatgtaatg atgcgggcaa cgaatgatcc agagggaaac actaaaggcg
    82921 gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga taaaaatagc
    82981 tcctcgttga ggagcagtac ataactatat tgacaactat aaaaattatt cgataaaata
    83041 gtgatgttat cgcatatctt cactatcacc cataaatagt cacactccaa gctatgcgat
    83101 aacaggtttg ttgccacaca ttctactggt tgattgttta tggctttatg tggcaacaac
    83161 c
//
LOCUS       DASHRQ010000003        82262 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.003, whole genome shotgun sequence.
ACCESSION   DASHRQ010000003 DASHRQ010000000
VERSION     DASHRQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.003"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            133..1287
                     /locus_tag="U2M88_000202"
     CDS             133..1287
                     /locus_tag="U2M88_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEM8372614.1"
                     /translation="MEYTKKRQRLAYIRLYSGVLHLRDSVRVSEKEKIKVTEMYTSIN
                     GELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVEPSKPEQ
                     REMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIELKE
                     PTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSF
                     QNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLTPIVLEQAFRKA
                     GTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDY
                     RNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            1385..1573
                     /locus_tag="U2M88_000203"
     CDS             1385..1573
                     /locus_tag="U2M88_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEM8372615.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1631..1984)
                     /locus_tag="U2M88_000204"
     CDS             complement(1631..1984)
                     /locus_tag="U2M88_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8372616.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2515..2937
                     /locus_tag="U2M88_000205"
     CDS             2515..2937
                     /locus_tag="U2M88_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM8372617.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2934..3164
                     /locus_tag="U2M88_000206"
     CDS             2934..3164
                     /locus_tag="U2M88_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8372618.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3662..3862
                     /locus_tag="U2M88_000207"
     CDS             3662..3862
                     /locus_tag="U2M88_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEM8372619.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3889..5082
                     /locus_tag="U2M88_000208"
     CDS             3889..5082
                     /locus_tag="U2M88_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM8372620.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5150..6715)
                     /gene="guaA"
                     /locus_tag="U2M88_000209"
     CDS             complement(5150..6715)
                     /gene="guaA"
                     /locus_tag="U2M88_000209"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEM8372621.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6774..7697)
                     /locus_tag="U2M88_000210"
     CDS             complement(6774..7697)
                     /locus_tag="U2M88_000210"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEM8372622.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7893..8498
                     /locus_tag="U2M88_000211"
     CDS             7893..8498
                     /locus_tag="U2M88_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM8372623.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8806..9822
                     /locus_tag="U2M88_000212"
     CDS             8806..9822
                     /locus_tag="U2M88_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEM8372624.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9963..11264
                     /locus_tag="U2M88_000213"
     CDS             9963..11264
                     /locus_tag="U2M88_000213"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEM8372625.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11310..11972
                     /gene="deoC"
                     /locus_tag="U2M88_000214"
     CDS             11310..11972
                     /gene="deoC"
                     /locus_tag="U2M88_000214"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEM8372626.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11993..12391
                     /locus_tag="U2M88_000215"
     CDS             11993..12391
                     /locus_tag="U2M88_000215"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEM8372627.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            12457..13539
                     /locus_tag="U2M88_000216"
     CDS             12457..13539
                     /locus_tag="U2M88_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM8372628.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            13751..15313
                     /locus_tag="U2M88_000217"
     CDS             13751..15313
                     /locus_tag="U2M88_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8372629.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15306..16439
                     /locus_tag="U2M88_000218"
     CDS             15306..16439
                     /locus_tag="U2M88_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8372630.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            16439..17395
                     /locus_tag="U2M88_000219"
     CDS             16439..17395
                     /locus_tag="U2M88_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8372631.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            17494..18660
                     /gene="deoB"
                     /locus_tag="U2M88_000220"
     CDS             17494..18660
                     /gene="deoB"
                     /locus_tag="U2M88_000220"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEM8372632.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            18684..19502
                     /locus_tag="U2M88_000221"
     CDS             18684..19502
                     /locus_tag="U2M88_000221"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEM8372633.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            19519..20226
                     /gene="deoD"
                     /locus_tag="U2M88_000222"
     CDS             19519..20226
                     /gene="deoD"
                     /locus_tag="U2M88_000222"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEM8372634.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(20524..21210)
                     /gene="gpmA"
                     /locus_tag="U2M88_000223"
     CDS             complement(20524..21210)
                     /gene="gpmA"
                     /locus_tag="U2M88_000223"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEM8372635.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(21382..21963)
                     /locus_tag="U2M88_000224"
     CDS             complement(21382..21963)
                     /locus_tag="U2M88_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM8372636.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(22096..22779)
                     /gene="rpiA"
                     /locus_tag="U2M88_000225"
     CDS             complement(22096..22779)
                     /gene="rpiA"
                     /locus_tag="U2M88_000225"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEM8372637.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            23098..23511
                     /gene="rpsL"
                     /locus_tag="U2M88_000226"
     CDS             23098..23511
                     /gene="rpsL"
                     /locus_tag="U2M88_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEM8372638.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            23595..24065
                     /gene="rpsG"
                     /locus_tag="U2M88_000227"
     CDS             23595..24065
                     /gene="rpsG"
                     /locus_tag="U2M88_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEM8372639.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            24159..26246
                     /gene="fusA"
                     /locus_tag="U2M88_000228"
     CDS             24159..26246
                     /gene="fusA"
                     /locus_tag="U2M88_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEM8372640.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            26418..27605
                     /gene="tuf"
                     /locus_tag="U2M88_000229"
     CDS             26418..27605
                     /gene="tuf"
                     /locus_tag="U2M88_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEM8372641.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            28012..28320
                     /gene="rpsJ"
                     /locus_tag="U2M88_000230"
     CDS             28012..28320
                     /gene="rpsJ"
                     /locus_tag="U2M88_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEM8372642.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            28351..28980
                     /gene="rplC"
                     /locus_tag="U2M88_000231"
     CDS             28351..28980
                     /gene="rplC"
                     /locus_tag="U2M88_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEM8372643.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            29008..29631
                     /gene="rplD"
                     /locus_tag="U2M88_000232"
     CDS             29008..29631
                     /gene="rplD"
                     /locus_tag="U2M88_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEM8372644.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            29631..29921
                     /gene="rplW"
                     /locus_tag="U2M88_000233"
     CDS             29631..29921
                     /gene="rplW"
                     /locus_tag="U2M88_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEM8372645.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            29960..30793
                     /gene="rplB"
                     /locus_tag="U2M88_000234"
     CDS             29960..30793
                     /gene="rplB"
                     /locus_tag="U2M88_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEM8372646.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            30836..31114
                     /gene="rpsS"
                     /locus_tag="U2M88_000235"
     CDS             30836..31114
                     /gene="rpsS"
                     /locus_tag="U2M88_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEM8372647.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            31137..31484
                     /gene="rplV"
                     /locus_tag="U2M88_000236"
     CDS             31137..31484
                     /gene="rplV"
                     /locus_tag="U2M88_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEM8372648.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            31498..32154
                     /gene="rpsC"
                     /locus_tag="U2M88_000237"
     CDS             31498..32154
                     /gene="rpsC"
                     /locus_tag="U2M88_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEM8372649.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            32157..32591
                     /gene="rplP"
                     /locus_tag="U2M88_000238"
     CDS             32157..32591
                     /gene="rplP"
                     /locus_tag="U2M88_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEM8372650.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            32581..32769
                     /gene="rpmC"
                     /locus_tag="U2M88_000239"
     CDS             32581..32769
                     /gene="rpmC"
                     /locus_tag="U2M88_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEM8372651.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            32794..33060
                     /gene="rpsQ"
                     /locus_tag="U2M88_000240"
     CDS             32794..33060
                     /gene="rpsQ"
                     /locus_tag="U2M88_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEM8372652.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            33118..33486
                     /gene="rplN"
                     /locus_tag="U2M88_000241"
     CDS             33118..33486
                     /gene="rplN"
                     /locus_tag="U2M88_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEM8372653.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            33523..33831
                     /gene="rplX"
                     /locus_tag="U2M88_000242"
     CDS             33523..33831
                     /gene="rplX"
                     /locus_tag="U2M88_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEM8372654.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            33859..34398
                     /gene="rplE"
                     /locus_tag="U2M88_000243"
     CDS             33859..34398
                     /gene="rplE"
                     /locus_tag="U2M88_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEM8372655.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            34417..34602
                     /locus_tag="U2M88_000244"
     CDS             34417..34602
                     /locus_tag="U2M88_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEM8372656.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            34638..35036
                     /gene="rpsH"
                     /locus_tag="U2M88_000245"
     CDS             34638..35036
                     /gene="rpsH"
                     /locus_tag="U2M88_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEM8372657.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            35068..35604
                     /gene="rplF"
                     /locus_tag="U2M88_000246"
     CDS             35068..35604
                     /gene="rplF"
                     /locus_tag="U2M88_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEM8372658.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            35765..36121
                     /gene="rplR"
                     /locus_tag="U2M88_000247"
     CDS             35765..36121
                     /gene="rplR"
                     /locus_tag="U2M88_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEM8372659.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            36142..36642
                     /gene="rpsE"
                     /locus_tag="U2M88_000248"
     CDS             36142..36642
                     /gene="rpsE"
                     /locus_tag="U2M88_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEM8372660.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            36657..36836
                     /gene="rpmD"
                     /locus_tag="U2M88_000249"
     CDS             36657..36836
                     /gene="rpmD"
                     /locus_tag="U2M88_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEM8372661.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            36880..37320
                     /gene="rplO"
                     /locus_tag="U2M88_000250"
     CDS             36880..37320
                     /gene="rplO"
                     /locus_tag="U2M88_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEM8372662.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            37320..38615
                     /gene="secY"
                     /locus_tag="U2M88_000251"
     CDS             37320..38615
                     /gene="secY"
                     /locus_tag="U2M88_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEM8372663.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            38677..39324
                     /locus_tag="U2M88_000252"
     CDS             38677..39324
                     /locus_tag="U2M88_000252"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEM8372664.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            39515..39733
                     /gene="infA"
                     /locus_tag="U2M88_000253"
     CDS             39515..39733
                     /gene="infA"
                     /locus_tag="U2M88_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEM8372665.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            39766..39882
                     /gene="rpmJ"
                     /locus_tag="U2M88_000254"
     CDS             39766..39882
                     /gene="rpmJ"
                     /locus_tag="U2M88_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEM8372666.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            39900..40265
                     /gene="rpsM"
                     /locus_tag="U2M88_000255"
     CDS             39900..40265
                     /gene="rpsM"
                     /locus_tag="U2M88_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEM8372667.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            40293..40682
                     /gene="rpsK"
                     /locus_tag="U2M88_000256"
     CDS             40293..40682
                     /gene="rpsK"
                     /locus_tag="U2M88_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEM8372668.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            40762..41700
                     /locus_tag="U2M88_000257"
     CDS             40762..41700
                     /locus_tag="U2M88_000257"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEM8372669.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            41728..42108
                     /gene="rplQ"
                     /locus_tag="U2M88_000258"
     CDS             41728..42108
                     /gene="rplQ"
                     /locus_tag="U2M88_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEM8372670.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            42380..43219
                     /locus_tag="U2M88_000259"
     CDS             42380..43219
                     /locus_tag="U2M88_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEM8372671.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            43195..44064
                     /locus_tag="U2M88_000260"
     CDS             43195..44064
                     /locus_tag="U2M88_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEM8372672.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            44054..44851
                     /locus_tag="U2M88_000261"
     CDS             44054..44851
                     /locus_tag="U2M88_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEM8372673.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            45054..45800
                     /gene="truA"
                     /locus_tag="U2M88_000262"
     CDS             45054..45800
                     /gene="truA"
                     /locus_tag="U2M88_000262"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEM8372674.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(46133..48802)
                     /locus_tag="U2M88_000263"
     CDS             complement(46133..48802)
                     /locus_tag="U2M88_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEM8372675.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWHLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(49040..49228)
                     /locus_tag="U2M88_000264"
     CDS             complement(49040..49228)
                     /locus_tag="U2M88_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEM8372676.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            49498..49860
                     /locus_tag="U2M88_000265"
     CDS             49498..49860
                     /locus_tag="U2M88_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEM8372677.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            49912..51594
                     /locus_tag="U2M88_000266"
     CDS             49912..51594
                     /locus_tag="U2M88_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEM8372678.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            51711..53747
                     /gene="recG"
                     /locus_tag="U2M88_000267"
     CDS             51711..53747
                     /gene="recG"
                     /locus_tag="U2M88_000267"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEM8372679.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            53796..54797
                     /gene="plsX"
                     /locus_tag="U2M88_000268"
     CDS             53796..54797
                     /gene="plsX"
                     /locus_tag="U2M88_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEM8372680.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            54919..55161
                     /gene="acpP"
                     /locus_tag="U2M88_000269"
     CDS             54919..55161
                     /gene="acpP"
                     /locus_tag="U2M88_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEM8372681.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            55429..56433
                     /locus_tag="U2M88_000270"
     CDS             55429..56433
                     /locus_tag="U2M88_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8372682.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            56434..57378
                     /locus_tag="U2M88_000271"
     CDS             56434..57378
                     /locus_tag="U2M88_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8372683.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            57378..58340
                     /locus_tag="U2M88_000272"
     CDS             57378..58340
                     /locus_tag="U2M88_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8372684.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            58367..59281
                     /locus_tag="U2M88_000273"
     CDS             58367..59281
                     /locus_tag="U2M88_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8372685.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            59307..61088
                     /locus_tag="U2M88_000274"
     CDS             59307..61088
                     /locus_tag="U2M88_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM8372686.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            61436..62122
                     /locus_tag="U2M88_000275"
     CDS             61436..62122
                     /locus_tag="U2M88_000275"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEM8372687.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            62142..65723
                     /gene="smc"
                     /locus_tag="U2M88_000276"
     CDS             62142..65723
                     /gene="smc"
                     /locus_tag="U2M88_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEM8372688.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            65732..66541
                     /locus_tag="U2M88_000277"
     CDS             65732..66541
                     /locus_tag="U2M88_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM8372689.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            66552..67550
                     /gene="ftsY"
                     /locus_tag="U2M88_000278"
     CDS             66552..67550
                     /gene="ftsY"
                     /locus_tag="U2M88_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEM8372690.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            67737..70004
                     /gene="gshAB"
                     /locus_tag="U2M88_000279"
     CDS             67737..70004
                     /gene="gshAB"
                     /locus_tag="U2M88_000279"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEM8372691.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(70093..70545)
                     /locus_tag="U2M88_000280"
     CDS             complement(70093..70545)
                     /locus_tag="U2M88_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEM8372692.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKSNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            70695..71645
                     /locus_tag="U2M88_000281"
     CDS             70695..71645
                     /locus_tag="U2M88_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEM8372693.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            71638..72597
                     /locus_tag="U2M88_000282"
     CDS             71638..72597
                     /locus_tag="U2M88_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEM8372694.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            72594..73349
                     /locus_tag="U2M88_000283"
     CDS             72594..73349
                     /locus_tag="U2M88_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8372695.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            73372..74325
                     /locus_tag="U2M88_000284"
     CDS             73372..74325
                     /locus_tag="U2M88_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM8372696.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(74969..75265)
                     /locus_tag="U2M88_000285"
     CDS             complement(74969..75265)
                     /locus_tag="U2M88_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEM8372697.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            75620..75976
                     /locus_tag="U2M88_000286"
     CDS             75620..75976
                     /locus_tag="U2M88_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEM8372698.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            75986..76885
                     /locus_tag="U2M88_000287"
     CDS             75986..76885
                     /locus_tag="U2M88_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEM8372699.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            77402..78133
                     /locus_tag="U2M88_000288"
     CDS             77402..78133
                     /locus_tag="U2M88_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEM8372700.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            78278..80305
                     /locus_tag="U2M88_000289"
     CDS             78278..80305
                     /locus_tag="U2M88_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEM8372701.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            80413..81510
                     /locus_tag="U2M88_000290"
     CDS             80413..81510
                     /locus_tag="U2M88_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEM8372702.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            81817..>82262
                     /locus_tag="U2M88_000291"
     CDS             81817..>82262
                     /locus_tag="U2M88_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8372703.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT        26975 a        14727 c        17563 g        22997 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
      181 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
      241 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
      301 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
      361 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
      421 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
      481 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
      541 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
      601 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
      661 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
      721 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
      781 tcatttcaaa atgcagttat ggaagggata cgctatggtt gcgaacaagg attatatggt
      841 tggaatgtga cggattgtaa aatctgtttt aagtacggtt tatactatag ccctgttagt
      901 actccagcag attttcggat gcttactcct attgtactgg agcaagcctt tagaaaagct
      961 ggaacagaat tgttagagcc atatcttagt tttaaagttt atgcaccaca ggaatatctt
     1021 tcacgggcat ataacgatgc tcccaaatat tgtgcaaata tcgtaaatac tcaactgaaa
     1081 aataatgagg tcattattat tggagaaatt cctgctcgat gtattcaaga ttatcgcaat
     1141 gatttaactt tttttacaaa tgggcttagt gtttgtttag cagagctaaa aggatatcag
     1201 gttaccactg gcgaacctgt ttgccagacc cgtcgtctaa atagtcggat agataaagta
     1261 agatatatgt tcaataaaat aacttagtgc gttttatgtt gttatataaa tatggtttct
     1321 tattaaataa gatgaaatat tctttaatat agatttgaat taaagtggaa aggaggagat
     1381 tgttattata aactacaagt ggatattgtg tcctatttgt ggaaataaaa caagactacg
     1441 aatacgagtg gatactatac ttaaaaattt ccctttatac tgccccaaat gtaagaacga
     1501 aactttaatt aatgttcaaa aaatgaatat aataacaatc aaagagccag acgccaagac
     1561 gcagagccga taatttgaga aatgaaactc tcatcttatc ggctcttttt gtttatctga
     1621 attttactga ctagccttca atatttcttg cttcattgat accttttgct agtgcttcca
     1681 ttaaggatag ttctttgtct gtaaagctat ccatgtattt ttctacctgt aaccttcgag
     1741 tgcttttaat caaattatct gtaggtaaga aaaattcatc aatagataca tggagtaatg
     1801 atacaagatc atacagcact tgtgtacttg ggtgttgtcc tttgttttct atatttgtta
     1861 aataacgtgg gtcaatttca atcattgttc ccacttgttc acgagtgaga ccccgtttca
     1921 ttcgagcctc tttaatcgct aaaccaacgg ctctaaaatc gtatttatct tcttttttac
     1981 gcatactaaa tcacctctat acattttact gttcctatat aaccataaac aggtatagaa
     2041 aaacatgtta tatagtttat gagttcctat agaacactgt ggaatttatt aatgcgatga
     2101 atccaatttc aaatagaaag gtcgctgatt ttatttacct atacaacttt ataaaattta
     2161 gtcatactcc tagaaagtag ataaaattgg cgacttaatg gagggaaaag tcgccatgag
     2221 aaagataatc tgtcatctag ttctcttaat taagttctta tcaaaaaaac ttatccacct
     2281 gtactggata gatatgacca tcaatgtggt tttgcagatt acatagatag aaaaatcaaa
     2341 gcatatgaac agagaaaaca atctgtttat gtgctttttt ggtcacttaa taaatatctt
     2401 ttaggatttc tcttcattta tgcaacaaat ccccctttcg tgtcggggtt tagtatgaag
     2461 ggagataaag aagcacatgc cactcccttt cacgacagaa agggggtgaa aagcatgaaa
     2521 ccatcttctt ttcaagaaac catagaaaat cagtttgact atatctgcaa acaagttatt
     2581 gaagatgaac gaaaagatta cttcaaacat ctagacagac tatcagcaaa agaagtctcg
     2641 ttttctgata tagataacta ccttgtcaac tctttttcta ctgttgatac gtacgtcact
     2701 gatttccaac tatttaaatt atttggatta agaatcaatg ttgaaagtga tttattaagt
     2761 gaagccctgc gtcatctttc agagaaaaaa cggaacatta ttttacttca ttactttatg
     2821 gatatgagtg atgtggagat tgcggagtta ctaaatttga atcgttcgac tgtttatcgt
     2881 catcgaataa gtggactagc catgattaaa gaattcatga aggagtgtaa agaatgaaaa
     2941 cagcttatcc aagggttccc ttccccctca ttgtaaaggc gacagatggc gacgtggaag
     3001 caattaatca gattgttaaa cattatcgag ggtatacctc aaaacgttca ttacgtcgca
     3061 tgacagacga atatggaaat tctcatatgg ttattgatga aaccctacgt ggcagaatgg
     3121 aaacgaaact catctctaag attttatcgt tcgaaattaa atagtcccat tttttcgtgg
     3181 aagcgtgtcg aatcgtatcc acgcttcccg atttgggagg tttgttattc catcaaagca
     3241 tattgtgtgt tcagtaggtt ttgataggct aacaagccaa acgttctttg acaaccaaat
     3301 aaaggcaatc gaatacgttt cggtaagaaa agagctgatg aactaacacg ccatgaccta
     3361 caaaattagt gagcgattca tgttagtaat tcgaaagtaa tctttagcag gattgctgcc
     3421 atgactttct tatcgtgata atgatactcc cgtatagcca tagtccgagc gttagaagcg
     3481 tcacaggcaa tgggtacggc tacatgagaa ccatgcagaa gtgaaattcc tgtgagcttt
     3541 gctagagctg ttcgattgct aagatattct tctcttgtca gtgtattatt ataaaactat
     3601 ttgatataat tataggtgaa tattggagtt ttcctagcct ataattggga aaggagtaat
     3661 aatgataacg gtcacaaaaa aggatttagt tgagctagga tatggaactt ctttttcagc
     3721 agacatcata aaaaaagcaa aagaattgat gattacaaaa gggcatatct actatcaatc
     3781 tcgaaagcta gatagagtcc ctcgtgaagc tgttgaagaa attctaggaa ttcggttttc
     3841 aaatgaaaac aggtctgagt agtttttgca cagtaaggag taaaaactat ggttaaagac
     3901 ccaataaaaa aagcaaagaa tggaacctat tattttcgtg caaatctagg ttatgattct
     3961 aaagggaaaa aaattcaaaa atatcgtagt ggattcacta ctaaaaaaga agcaagagaa
     4021 gaatattcga aactgctatt gatgaaacca gaagaattaa gtgagaataa ggataaaatg
     4081 acgtttaaac attatatctt tgaaattttc cttccatggt ataagacaca ggtaaagctc
     4141 agaacatatg aaaatcgctt accaactatt aagaagcatt tctcttattt tgataaaatg
     4201 gctgtttccg atattgagcc tattgatgtg caaaattggc aactaaaatt atcaaagaag
     4261 tgtaagtctt cttatgtaag agctgttcaa ggactatttt cagttgctat ggatcgagca
     4321 attgtccttg ggataaccac cacaaatcca tctaagatta ttggcaatgt gaaaaagcaa
     4381 aagtctaaaa ttgaattttg gacaaaagaa gaatttgaaa aagtaatctc tctaatttac
     4441 aaagaagatt actatcaaca ctttttattt atctctctgt ggtttttatt tatgactgga
     4501 atgcgaattg gtgaagctac tgccattcaa tgggaagata ttgattttga ctcaggagta
     4561 ctaaccatca acaaaacact ctattacaag aatcaatcga attatagatt tgttgaacca
     4621 aaaacaaagg cgagcgttcg tcatattgta ttagataaat gcacattaac ttatttatcc
     4681 gaatggaagg aagtacaaca aaatctaatt cagacagact ttgtaatgag ctataacggt
     4741 attcctaccc aaaaacatac actatcatat gctattgaac gctatgctaa gctggctggt
     4801 atccatcgaa tcagaatcca tgggttacgg cattctcatg cttccttgtt aatcagtatg
     4861 ggagaaaatc cacttatcat taaagatcgt ctaggacacg aagatattga gacgacttta
     4921 ggaacgtatg gacatcttta tcctaacagt aatttcgagg tagctaacaa attaaatgga
     4981 gccatttcct ttaaagaagc aaaacaaaat tatgatactt caccaaagaa tcaatttact
     5041 gtagactact tgcgaaacaa agaaatgaaa aaagtgcaat aaaagtgcag taaaaagata
     5101 agaagagcca gaaacccttg gtataagagg atttctagct ctcctacaac tattcccact
     5161 ccactgttgc tggtggttta gaagtgatgt catacacgat acggttgacg tgggctactt
     5221 cgtttacgat acgtacagag attttttgca agacttccca tgggatacgg gcaaagtcag
     5281 cggtcattcc gtcgattgaa gttacagcac ggataccgac tgtgtagtcg taagtacgtc
     5341 cgtcacccat aacaccgaca gagcggatcc ctggaagaac agtgaaatat tgccagatat
     5401 cacggtcaag tccagcagct gcgatttctt cacgcaagat agcatcagat tcgcgtacga
     5461 tttctagctt gtcttcagtg atttcgccaa gaacacgaat accaagacca ggaccaggga
     5521 atggctgacg ccaaacgatt gaatcaggca tgcctaactg agtccctaat tcacggactt
     5581 cgtctttgaa taatgtgttt aatggttcga tcaattcaaa ttgcatatct tctggcagtc
     5641 cacctacatt atggtgagat ttgatcgtct gtgctgtttc tgtaccagat tcgataacgt
     5701 cagtatacaa cgttccttgt gctaggaaag agacgccttc ttcaccagca agttttgttg
     5761 cttcgtcatc aaaaacatat acgaattcat tcccaatgat tttacgtttt tgttctgggt
     5821 cagatacacc tttcaacttg tctaagaagc gatctcttgc atcgactttg atgatgttca
     5881 agccgaattt tccgcctaga ctttccatta cttgttccgc ttcgcctttg cgtagcagac
     5941 cgtggtcaac aaagatagat gtcaattggt caccgatcgc tttttgtaaa agaaccccaa
     6001 cgacactaga atcaacgcca cctgaaagac caagcaagac ttttttgtca ccaacttgtt
     6061 cacggatttt ttctacttgc atatcgataa agttatccat tgtccagtta ccttcacagt
     6121 gacaaacatc aaaggcaaag tggcgcagca attcgattcc gtattcggaa tgacgtactt
     6181 ctgcatggaa ttggatacca taaaaattac gtgttgtgtc ttggatagaa gcaatcggac
     6241 aatctttgct tgttccgact gtttcaaagc cttcaggaac tttagttacc aagtcaccat
     6301 ggctcatcca tactgtttgt tgttttggtg tttctttaaa caagacagct tgatcgcttg
     6361 ttacttctaa caaggctttc ccatattcgc ggtttttagc aggctctacg gaaccgccaa
     6421 gtttgtacgt gatcaattgc ataccatagc aaatccctaa tacaggaata cctaaattga
     6481 agatctcagg atcgatccca aatgcattct catcatatac actgtttggt ccgccagaaa
     6541 gaatgattcc ctttggattc atcgctttta cttcttctgc agtaatgcgg tggctcaata
     6601 attcggaaaa gactccaaga tcacgaatac gtcttgtgat caattggtta tactgactac
     6661 cgtagtctaa tacgataatc ttttcaacgt tcgtcatatc ggcaacgttt gtcaaattta
     6721 ttacccctat ccctttaaat tttatctcaa aatagtcaaa ctattttaaa atgctaatat
     6781 tttcgcatga aaatcgaatc gatgatatga ttttcagttt tatggagaat tatatcggct
     6841 cttcctcgtg taggcaagat atattcctgt aaatttttca aattcacatt tttccataca
     6901 tcttttgcca ttttaaaggc ttcttcacga tctccgatag caaaacgata ataataattc
     6961 tctggatttt ggaaagccgt atccagtaac gcaccaaaac gatccaaata ccatttttcg
     7021 atcagcacgg gatcggcatc gacatagaca gaaaaatcaa aaaagtcgct gacgtagatt
     7081 tgctgattcg caggtaattg aagcgtattg atcccctcaa cgatcaagat atcaggctgc
     7141 tggatcaatt cgtacttgtc tggaatgata tcatacacat catgtgagta aacaggtact
     7201 tttatctctt cctgaccaga ttttacacgg ttcaagaaat cgatcagcat ctccatatca
     7261 taactttcag gaaatccttt acggtccatg attccacgct cttttaatat acgatttgga
     7321 taaagaaatc cgtcagtcgt gatcagctga acgtttcttc gtttgaaggt acgagacaaa
     7381 atcgtctgca gcagtctggc tgttgtactt tttccaacgg ctacacttcc ggcgatcccg
     7441 ataataaaag gcggtacttc tacgtattta tgaagaaata aacctttact caagaacaaa
     7501 gattcaaatt ctttcatata aagataaatc agatgagcaa gaggtacata gatatctcga
     7561 acatcctgca tggaaatgcg gtcattcaaa cttttgatat tatctagttc atcttgtgtc
     7621 aaaggtgctt gcccattacg ataaaattct cgccattctt ctcttgccac acggtagtag
     7681 ttcattggat cattcatatt agcctcctgc gttctcccat caatggaact attttaacac
     7741 aaaataaacg caaaccccac cacttaagag agattgccga tctttttgta taatctttat
     7801 aatagaaaga aacggagtct tattcttgac ttgcgcacgg atacataaaa ggatagactg
     7861 ttgtcatgaa agaaatgaga aggatggaag acatgacaaa tcattactat tctgaacgcc
     7921 cagatacggc gcacgatttt gaacaatggg cttttgagtt aaaaggcaga acctttcaat
     7981 ttgtaacaga ttctggtgta ttttcacgtg aaacagtgga ttatggttca cgtgtcctga
     8041 ttgatgcttt cgattgggaa gagttacctg atgaaggaaa gatcttagat gtgggatgcg
     8101 ggtatggacc gattgggtta gctttggcat ttgcgacaca gcggtttgtt gaaatggtcg
     8161 acattaatga acgagcggta agtctagcgc aaggaaacgc aaagcgtaac caaatcgaac
     8221 atgttgatat ccatcaatcg aatatctatg aagcagttca tgaagaaacc tatgcagcta
     8281 tcgtaagcaa tccgccgatc cgtgctggaa aaaaagttgt acatgaaatt ttgacaggcg
     8341 catatcctcg gttgaagaag ggcggtacac tgacaattgt gatccagaag aaacaaggag
     8401 cgccgagtgc gcaaaagaaa atggaagaaa catttggtaa tgcagaaatc gtcacaaaag
     8461 ataaaggata ttatatcatc aaaagtgtga aagaataact agctgaaaaa tagtggagcg
     8521 acgaaagagt ggaactgggc gtgcatgtac gggtacgtat cagttctttt tttgttgaga
     8581 agtttttaaa aacaaagaac tattttttct tcaaaaagaa atagtaggaa cagagtcgtt
     8641 gttttttacg ataaaataaa taagatacag aacttgtata gaaggctgcc aagtcggtag
     8701 ataaaggagg tttttatcaa aaaacatact ttcctttttt ctctattctt ttcttggggg
     8761 ttttggtaca ctgataggga atttagatag aagggagcca ataggatgaa gctgaccatc
     8821 agagatatcg cagaaatggc aggcgtatca gtaacaactg tctcacagat tttgaataac
     8881 aaaggaagca gatttagtga taagacaaga aaaaaagtgt tgtcgatcgt gaatgaacat
     8941 cattataagc ccgattattt cgcttcaaat gtgatcaatc gacattcaaa gaccatcgga
     9001 atgatcgtcc ctgatgtcac tgatttcttc ttctccaaac tgatcgaagg tgtagagagc
     9061 tatttgaatc ctttgggcta tgtgatcatg ttatgcaatt ctcgtcatag ccaagaaaat
     9121 gagatcaagt atttacaaga gctttcccac cgctcagtgg acggaatctt gctggccaca
     9181 ccaaatattt tacctgagaa atatgcatta gacagcggtt tttatcaaaa aatgccaatc
     9241 attttaatcg atcgcggatt gaatcgtcgg gataacggac ggcttatcgt aaaggaatat
     9301 gagggcgcct atcaagctgt ttcgctgttt attgaaaatg gccatacgaa gatcggcatg
     9361 ctgaaggaaa ccactggata ctatcaatta gaagaacgtt tcaacggata tcgtcatgcg
     9421 ctgaaagatc atggcctgcc ttttaatggg aaatttgttg aacatggaga gttgactgtc
     9481 caaggtggat atactgcatc aaaaaaatta ctcaaacata aagatatcac ggctattttt
     9541 tgcgggaatg atgccatggc gatcggatgc tatcaggcga tcgatgaatt gggcaaaaaa
     9601 atcccagaag atatctcagt cataggattc gatggattaa agctgtcaga atacatgatt
     9661 ccgcaattga cgacagtgca gcagccaagt tttgatattg gattttatgc tgcacgtttc
     9721 ctgatcgata cgatcgaatt tcctcaaaga aaagttccga acaaagtgtt cgaaacaaaa
     9781 ttaattattc gtgagagcgt aaaagtattg acaaagggct aactcggcgg tatattgctt
     9841 atgtaagcat ttacaaaggc tagttagtgc tttttttatt tttagtaaac cagtttacta
     9901 aagtggttta cctttcctaa aaataatgta taataccgaa tgaagacaag gaggatcagg
     9961 caatgcgaat ggtggatctg attgaaaaaa agagggatgg aaacgagctg tcgaaagagg
    10021 aaatcgagta catcgtcacg aactatacga atggaaagat tccagattat caagtcagtg
    10081 cattattgat ggcaattttt tatcaagaca tgacaaatga agaaatcacg aacttaacat
    10141 tggcgattgc taattctggt gatgtcatcg atttgagttc tcttgaagga ataaaggtag
    10201 ataagcattc gacaggtggc gttggtgaca cgaccacatt gattttagcc cccctagtag
    10261 ctagtgtcgg agtgacagtg gctaaaatgt ctggacgcgg tctaggttac acaggtggta
    10321 cactagacaa gctagaagca attcccggat ttcagatcga gctttcggat gaagaatttg
    10381 tacgcattgt taatgaaagt aaagtagcgg taattggtca atcaggaaac ttggcaccgg
    10441 cggacaaaaa attgtatgcc ttgcgtgacg tcacagcgac cgttgattct cttccgctga
    10501 ttgccagttc gattatgagt aaaaaaatcg cggcaggagc agatgcaatc gttttggatg
    10561 tgaccactgg agacggtgct ttcatgaaga acatcgaaga tgcaagacgt ctggcaaaga
    10621 ccatgacgag cattgggaaa ctggctaacc gagaaacagt ggcggttatc tcagatatgt
    10681 cagaacctct tggcgaagct atcggaaata gtctagaagt tgtcgaggca atcgaaacat
    10741 tacaaggaaa tggtccagaa gatctagtag aaatgtgcta cgccttagga agccaaatgg
    10801 ttgttcttgc tggcaaagct aagacaaccg atgaagcaag aacgttgctt caagaagctc
    10861 tggaatcagg taaagcgtta gcaaaattca aagaaatgat ccaaaatcaa gggggagacc
    10921 cagcgatcgt ggaacatcca gaacgaattt tgactgcacg ctacacaatg gagcttcctg
    10981 ctaaacaaag cggagttgtg tcaaaaatcg tggcaaatga attaggaatt gctgcaatga
    11041 tgctaggtgc tgggcgtaaa accaaagaag aagatattga tcacgctgtt ggattgaaac
    11101 tgcataaaaa aattggtgac actgtgacaa aaggtgagtc actattgacg atctatagta
    11161 acgacgaaga aatcacttct gtcattgaat tattatataa aaatattgaa attggtgaat
    11221 cggcaatgaa gccaacgttg attcatgaca tcatcacaga ataaaggaaa aaagcggact
    11281 ttaccgtttt aaagaaaagg ggaaaagaaa tggaactaaa ccgaatgatc gaccatacta
    11341 ttttgaaagc agacgcatct aaagaagctg tgatgcaaat tatcgaagaa gcaaaaaaat
    11401 accattttta ttctgtctgc atcaatccag catgggttgc gctagcgaaa gaacaattac
    11461 aaggtgagcc agtagccgta tgtacagtaa tcgggtttcc tctaggagca aacacacccg
    11521 agacaaaagc atttgaaaca acagatgcca tcaataacgg tgcagatgaa gtcgatatgg
    11581 tcatcaatat cggtgctttg aaatcaaagg atgatcaaca agtccaaaaa gatatcgaag
    11641 cagttgttga agcagcaaaa gatcgtgcgc ttgtcaaagt tattattgaa acatcgttat
    11701 tagatagaga agaaatcatc cgagcttgcg aaatagcaaa agctgcaggc gctgattttg
    11761 tcaaaacatc aactggtttc tcaacaggcg gagcaaaggt agaggatgtc cgtttgatgc
    11821 gtgaaactgt aggaccagaa atgggtgtca aagcttccgg cggtatccat aacgaagaag
    11881 aagcaatggc aatgatcgaa gctggtgcta caagaatcgg aacaagcgca ggagtagcaa
    11941 tcgtatctgg ttcaactggt gaaggctatt aattttttca aaggagaagc tcatggcaga
    12001 actcaaacaa gaatggatca acacagctat tgaagcgtta gataaagcct atgttcctta
    12061 ttcccatttt ccggtaggcg cttgtctcgt aacggaatca ggcaagattt atcaaggcat
    12121 caacattgag aatgcatcat ttggattaac gaattgtgcc gagcgcacag cttttttcaa
    12181 agcagtatct gaaggagaaa aaacatttac gcatctagtc gtagcggggc acacccccga
    12241 tcctatctct ccttgtggtg catgtcgaca agtaatggca gaattctgtt cccctgatat
    12301 gccggttacg ctagtaggag acaatggtgt gacgaaggca acgaccgttc gtgcactgct
    12361 gccttatgca tttactgaaa aagatctata actcatgttc attcagtcag ctgactgttg
    12421 aaaaataaaa caattatttt aggaggcttt acagaaatga aaaaagcaaa attatttggt
    12481 ttaggtgctg tcgcactagc agcagggtta tttttaggtg catgcggcaa caacggctct
    12541 actgattcat ccggtggcaa aagctcatca gacacaacaa ctgcagcatt gatcacagac
    12601 acaggtggtg tagacgaccg ttcattcaac cagtcagctt gggaaggact agaaaaatgg
    12661 gggaaagatc aaggactttc acgaggaaat gatggcttcc aatatttcca atcttcaaat
    12721 gaatcagatt acatcccaaa tattgaccaa gcattgaatg ctggattcaa aacgattttt
    12781 ggtattggtt ataaactaaa accagcgatc caagaacaag caacaaacaa tacaggtacg
    12841 aactttgtta ttatcgatga tgtcatcgat ggattagata acgttgtatc tgcaactttc
    12901 aaagacaacg aagcttctta tcttgcaggt gttgcagcag cttatacgac tgaaacaaac
    12961 gtagtcggct ttatcggcgg tgtaaaaggt gaagtaatcg atcgttttga tgctggattc
    13021 aaagccggtg ttgatgcagg tgcgaaagaa ttaggaaaag aaatcaaagt attgaatcaa
    13081 tatgctggtg acttctctgc tccagataaa ggacgttcaa ttgctcaagg tatgtatgca
    13141 caaaacgcag atatcatctt ccatgcttca ggcggtacag gtaacggtgt attccaagaa
    13201 gcaaaatcat tgaacgaatc aggcgacaaa aaagtttggg ttatcggtgt tgaccgcgac
    13261 caatcagacg aaggcgaata cacattaaac ggcgaaaaga aaaacttcac attgacatct
    13321 acactgaaag cagttggaac agtagtagaa gatttggcac aaaaatcagc ggacggtaaa
    13381 ttccctggcg gcgaacacac tgtttatgga ttgaaagaag acggtgttgg tctaacagaa
    13441 ggacaacttt ctgacgaagc gaaaaaagca gttgatgaag caaaagaaaa aatcatttca
    13501 ggtgacgtaa aagttccaga aactcctgaa gaaaactaaa accatttcta tagccttgtt
    13561 agacacatgt ttgacaaggc tatggtttga ttagaacaag aattgttcat ttatccaagc
    13621 tgccttgtga cagtttggat aagggaacaa ctccctgaag taatgtttgt ttcaaaagaa
    13681 gaacgctcac taagcgtgaa tcttagtcaa tcgttgccct agatagaaat cgttaggatg
    13741 tgaaaaaaag gtggttcaag aaaatattgt catagaaatg cagaatatta ccaaagaatt
    13801 tggaacattc aaggcaaatg acaacatcaa tttacaagta aaggcaggag aaattcatgc
    13861 gttgcttgga gaaaacggtg ctggcaaatc tacattgatg aacgtgcttt ccggattatt
    13921 agagccgaca tcagggaaaa ttttgatgcg tgggaaagaa gtaaagatca caagtccgac
    13981 caaagccaac caattaggga ttgggatggt ccatcagcac tttatgcttg ttgatgcctt
    14041 tactgtaaca gaaaacatcg tgttgggaag cgagcctagt cgcgcaggga tgcttgacca
    14101 taaaaaagca cgaaaagaga tccaaaaagt ttctgaacag tatggattgt cagtcaaccc
    14161 agatgcttac gttcgtgata tttcagttgg aatggaacaa cgggtagaaa ttttaaaaac
    14221 actttaccga ggagcagatg tactgatttt tgatgagccg acagctgtat tgacccctca
    14281 agaaattgat gaattaatcg tgatcatgaa ggaattagtc aaagaaggca agtcgatcat
    14341 tttgattacg cataagttag atgaaatcaa agcagtagct gaccgctgta cagttatccg
    14401 ccgtggaaaa ggaatcggta cagtcaacgt taaagacgta acctcacaac aattagccga
    14461 tatgatggtt ggaagaacgg tttcattcaa aacgatgaaa aaagaagcga agcctcaaga
    14521 agtcgttttg tctattgaaa atctagtggt gaaagaaaat cgtggattag aagccgtgaa
    14581 aaacttgaac ttagaggttc gtgctggcga agtacttggt atcgctggaa tcgatggaaa
    14641 cgggcagtcg gagttgatcc aagctttgac tggtttgcga aaggcagaaa gcggacatat
    14701 caagctaaaa ggggaagaca tcactaataa aaaaccccga aaaatcactg aacatggtgt
    14761 aggacacgtg ccagaagacc gtcataaata cgggttagtc ctagatatga cattatctga
    14821 aaatatcgca ctacaaacct atcatcaaaa accttacagt aaaaacggca tgctgaatta
    14881 ttcagtaatg aatgaacatg ccagagaatt gatcgaagaa tatgatgttc gaacaacgaa
    14941 tgaacttgtt cccgcaaaag ctttatcagg cggaaatcag caaaaagcaa ttatcgctcg
    15001 tgaagtcgac cgagatcctg atctgttgat tgttgcaaat ccaactcgtg gactggatgt
    15061 aggtgcgatc gaatttattc ataaacgtct gatcgaacaa agggacaaat acaaagcagt
    15121 gttactgatc agtttcgaat tagaagaaat tttaaatgtt tcggatcgta ttgctgttat
    15181 ccatgaagga gaaattgtcg gtatcgttga tccgaaagaa acatctgaaa atgaattagg
    15241 tctattgatg gcgggctata ccttagaaga agcacgaaaa gagttagaaa agggggctgg
    15301 cgcacatgaa tgatcgcagc gaaaaattcc gtaatttatt agtgcctgta ctttcagttg
    15361 ttttaggatt gatcttaggc gcgatattga tggctgcttt tggttttcag cccatccaag
    15421 gttattcttc tatgctagat gcagcccttg gttcgcaaag agggataggt gaaacgttaa
    15481 ggcaagcaac gccgttgatt tttacagcat taggtttttc tgtagctaac tcagctgggt
    15541 ttttcaatat tgggctttct ggacaagcac tttgcgggtg gatcgtcagt gtctgggtgg
    15601 cacttgcgtt tccagatctg ccaaaagttg ttttgcttcc gttatgcgtg atcttaggtg
    15661 ccttagctgg tgcgatggca gcggctgttc caggtttact tcgagcattt tttggaacaa
    15721 gtgaagtgat cgtaacaatc atgatgaact atatcttatt atacgtaagc acatttgctt
    15781 tacatgaagt gatgcctgaa agctatcgct caagtttgga ctcttccaat atgatcagtc
    15841 aaaatgcctc actgcggtta ccatggttaa cacaagcttt tggcggctct cgcgtaaact
    15901 caggattatt tttagcaatc atcgcgctta ttttcgtttg gctgctgatg aagaaaacga
    15961 ctttaggatt tgaaatccgt tctgttggat tgaatccttt tgcttctgag tatgcaggaa
    16021 tgagtagtaa acgtacgatc gttttgtcga tggtcatttc tggcgctttg gcagggcttg
    16081 gcggtgtggt tgaaggatta ggaacgtatc aaaacttctt tgttcaaacg acttcgctat
    16141 ctattggatt tgacggaatg gctgtttcct tgcttggttc tggatcagca atcggtattc
    16201 ttttatcagc gatcttgttc agtattttga agatcggtgg acttggcatg cagacaggag
    16261 caggtgttcc gtttgagatc gtcaatgtat cgattgcttt gattatcttc tttgtcggga
    16321 tcaacttcct gattcgtttc gtcatggcta aagtcttcca agggaaaaaa caagaagaga
    16381 tcgttgcgac aatagaatcg aaaccaacga atcaaaacca tgaagggggc gaactataat
    16441 gtcgactatc gaattaattg ccttgatcct tacttctaca ctggtttatt caacaccgct
    16501 aattctaacg tcactaggcg gaactttttc tgaacgaagc gggatcgtaa acgtagggct
    16561 agagggaatc atggtcatgg gtgcttttag ttccatcgtt ttcaacttga cgatgagtcc
    16621 atcacttggt tctatgacac catggatcgc tattttagtg ggtggcgtcg taggtatcct
    16681 attttctctg attcacgcag tagcaacagt cagtttgcgt gctgaccata ttatcagcgg
    16741 gacagtaatt aatctgatgg cacctgcttt aggtgttttc ttgatcaaag catggtatgg
    16801 taaaggtcaa actgataaca tcacgcagaa tctggggtat ttttctttcc caggattagc
    16861 agatattccg gtcattggac caattttctt taaaaataca ttcttacctg cttattgtgc
    16921 tattttgatt gctgtcctag cttgggtgat catcttcaag acgaaattcg gcttgcgcct
    16981 ccgttcagtt ggtgaaaatc ctcaagctgc cgacacatta ggtatcaatg tatatgctat
    17041 gcgttattca ggcgtactga tttctggctt gttaggcggt atgggcggtg ctgtttttgc
    17101 tcagtcgatt tccggtaact tttcagctgg tacgatcgtt ggacaagggt tcatctccat
    17161 ggctgctatg atctttggta agtggaatcc tttaggggca atgggtgcct cattgttctt
    17221 tggttttgct caaagcttga gtatcattgg cgcacaatta ccagttattt cttcgattcc
    17281 gccagtattg ctgcaaattg ctccatattt gctgacaatc attgtgctgg ttgttttctt
    17341 agggaaagct tctggtccaa aagcaaacgg gaagaactat atcaaatcaa aataatcttt
    17401 tagcgaataa aaagggtggg ttacgggaag tgtttttcgt ctcgcccttt ttgttttaaa
    17461 ggcaataaaa ataaaaagaa agaaggaata atcatgttta aacgcgtaca tctgatcgtc
    17521 atggattcag taggaattgg agaagcacca gatgccgata agttcggtga tgttggttct
    17581 gatacattag gacacattgc gaaagaagca ggattgaacg ttccacattt agagcagctt
    17641 ggtttaggaa cgatccgtcc tcttgaaggt gtggaatcag tgaaagatca tcaaggctat
    17701 gcgacgaaat tagaagaagt ttctgtaggg aaagatacga tgacaggcca ctgggaaatc
    17761 atgggattga atatcaaaac tccatttcgt gtgtttccta atggattccc tgatgatctg
    17821 atcaaacaaa tcgaagaatt ttctggccgt aaagtcgtat gtaacaaacc ttatagcgga
    17881 acagctgtta ttgacgatta cggtgaacac caaatgaaaa caggcgattt aattgtatat
    17941 acttctgccg atcctgtcct tcagatcgcg gcacatgaag aaatcattcc tttagaagaa
    18001 ttatatcgga tttgccaatt cgttcgtgat ataacaaaag atgatccgta tatgattggc
    18061 cgaatcattg cacgtccgta cgtgggtgaa ccaggagact tcacacgaac aagcaatcgc
    18121 catgattatg cgttgaatcc attcggacac acagtccttg attctttgaa agaagcagga
    18181 aaggacgtga ttgctgttgg aaagatcaac gatattttca acggacaagg aatcactgaa
    18241 tctgtccgca cgaaaagcaa tatggatggc gttgaccagc tgctgaaagt tatgaaaaac
    18301 gatttttcag gaatcagctt taccaattta gtagattttg atgcattata cggtcatcgt
    18361 cgggatgtag tcggctatgc gcatgcgatt gaagaatttg acttacgaat tccagaattg
    18421 cttgaagcga tggaagaaga cgacttgcta atgattacag ccgatcacgg aaacgatccg
    18481 acgtttactg gtaccgatca tacgcgagaa tatgtaccgt tattggttta cagtaaaaaa
    18541 atgaaagaac aaggggcatt gcctcaagga tattattcag atatttcagc aacgattgct
    18601 gagaacttta gtgtttcttc gactgaaaat ggcgaaagct tcttgaaatt attaaaataa
    18661 tctttttaaa taggagaaag caaatgacca agttaagcga gatgttacaa gaaacgaccc
    18721 agtttatcct tgataaagga gtaaaagaaa ttgattttgg tttgattcta ggttcaggat
    18781 taggtgaatt agcagatgaa atcgaagatg ctgtaatgat ttcttatgat gagatcccgt
    18841 tctttcctgt ttcgacagta gttgggcatg caggaaagct agtttatggg tctctggcag
    18901 gcaagaaagt gttagccatg caagggagat tccactacta tgaaggtcat tccatgcaaa
    18961 cagtgaccta tccagtacgt gtgatggccg gactaggggc ccattcagtc attgtgacta
    19021 atgcatgtgg tggtgtcaac gaatcatttg taccaggaga tttgatgctg attaccgatc
    19081 atatcaattt tactggtgaa aatcctttga tcggtccgaa tgaagatgca atgggcccta
    19141 gatttccaga tatgagtcaa gcttatacgc ctgactatca agcaacagca aaagcagcag
    19201 cagaaaaact aggtttggat cttaaagaag gcgtatatat gggctattct ggaccaactt
    19261 atgaaacgcc tgcggagatc cgaatgtctc ggacaatagg agccgatgca gtagggatgt
    19321 cgactgttcc agaagtcatc gtagcagctc acagcggatt aaaagtgtta ggaatctctt
    19381 gtattaccaa cttagcagca ggtatgcaag cgaatctgag ccatgaagaa gttgtagaaa
    19441 caactcagag agtcaaacaa tcttttaaag cattggttaa agaaacattg acattattgt
    19501 aataggagga acaataaaat gagtgtgcat attgaagcga aagaaggcga gatcgccgat
    19561 aagattttac ttccgggaga tcctttgcga gctaaatata ttgcggagac ttttttggaa
    19621 gatccagttt gttacaatca ggtacgcggt atgttaggct ttaccggcaa atataaagga
    19681 gaacgtattt ctgttcaagg aacaggaatg ggaatgccat cagccaccat ctatgcccat
    19741 gaattgatcc agtcgtatgg tgtaaagaaa ttgattcgag taggtacatg cggtgcattg
    19801 tcaaaagacg tccatgttcg tgatttagta ctagcacaag gtgcagcgac cagttcttcg
    19861 atgatcgaaa aaaatttcca agcctttcat tttcctccaa tcagtgattt caatctatta
    19921 ctgaaagcat atgagatagc taaagaaaag ggctatacgg ttcatgtagg aaatgttctt
    19981 tcggaagatt ctttctacaa agatgatttg acagagacgt tccaattagc tgaacttggc
    20041 gttttaggag tggaaatgga agcagcggct ctttattact taggcgcgaa ataccatgtg
    20101 cagacattaa gtttgatgac cgttagcgac catttgatca ccggtgaaga aacaacagct
    20161 gcagaacgtc aatctacgtt caatgaaatg atcgaagtag gattggaaac agcgatagca
    20221 gaataagaag gaaatgaaaa agctgccctg cattaagtaa taagaacatc caagcaaaaa
    20281 tagctcctca tgtttttgac ttggctggac ttattattgc aaatgtaagc ttttgaacac
    20341 cgtttaccaa gaggttgtga atctgccgtt taactctgag tcacaaggaa caatttcaaa
    20401 aaacagcttt tcatgtttga tgaatattgt gacttgtgac cgagaagttg gcaggagaaa
    20461 accgtttata cgaaaaaagg gactgtgaca aatcgtgtca cagtcccttt tttatagtgt
    20521 tttttatagg tagtattttt tagcaactgt taagtcatca tttaattcat aaacaagtgg
    20581 ttgacctgtt ggaatttcaa gatccatgat gtcttcgtca gagatacctt cgatatgttt
    20641 agctagggcg cgtaatgagt taccatgtgc agctactaaa acagttttgt tgtctagtaa
    20701 tgctggagca atttcatctt gccagaatgg caaagcgcgt tctaatgtca ctttcaagtt
    20761 ttctccacca ggaatatcgc gtttgtctaa cattgcgtaa cgacggtcat ttgctgcaga
    20821 accttcgtca gtttcttcca ttaatggagg aagagtatcg taagaacgac gccagatgtg
    20881 cacttgttcg tcgccgtatt tttcagctgt ttcttttttg ttcaaacctt gtaatttacc
    20941 atagtgacgt tcgtttaagc gccatgattt gatttgtggt acccaaagtt gatctgaata
    21001 ttctaagatc aagttacatg ttttgattgc acgagttaaa acagaagtgt aagcaacatc
    21061 aaattcgatt ccagcttctt tgattttgcg tccgccttct tttgcttctt cgattccttc
    21121 tggtgctaga tttacatcag cccaaccagt aaattggttc aatgcattcc attcactaag
    21181 accgtgacga gaaaaaacta attttggcat gtttagctct ccttttaaga taggtttata
    21241 tttctgaaat tttcagttct gttttattgt acaatttttt ggaagagatt tccatcgaaa
    21301 aattagataa gattctatga gaaaacaagt agagaatata aaaaaacgaa cagtgcccac
    21361 gcaactgttc gtttttaggt attagatgca taattgctcg atttcttttt caggtaaatt
    21421 gatatattta taacatgttc caactgaaac accgcatttt gaagaaataa attggatcgt
    21481 ttcttttttt tcataataga gacgtcggat ttttttgatc gtttttgcat cgatttttgg
    21541 tcttccgccg atttttcctt ttttacgagc ttcatctaat ccaactaatg tacgttcttt
    21601 gatcaatgcg cactccatgt ttgctaggtt ttccattaat tggaaataga tttttcccat
    21661 ttcgcgcgta tcgattcctt catcaagact aatcaagtgc agcccgcgat ctttgaataa
    21721 ttgcgtgagt tctgttaatt gccgcgtaga tttgcctaat cgatgaagtt ggcaaatgac
    21781 tagtgcatcg ccttgttgca tgtcagcgat cactgactcc aaatgaacag cagtagaatc
    21841 attttgttcc acttcatatg tctcatggaa aatttcttga caaccaaagt cagataattg
    21901 atcaacctga gttgttaaat caacgtcatt tatggttgtg cgtgcataac caataatttt
    21961 catggtgatt cctttctagc agtgattttc taactggata taacagtaaa aatgtagcat
    22021 aaagtacgtc aaaaagatag ttttttcgca aattttccgc tgtctttttt tattggatga
    22081 tcaagcagtt tttctttatt tacgacggat tgcgtcaaga atctcaacgc ctttttctga
    22141 gccgacaact actttagaaa ccatgtttag gaaaagccca tgttctacta ccccaacaag
    22201 ttcatctaaa taggtagcta agctttctgg ctgttcgatg acttctagat gcagatcaat
    22261 gatgtaatga ccaccatctg tggttaagac ttctccgttt tcgtctgtac gcaagacggg
    22321 ttggaatctt ttttcttcaa aaatatgaag tagctgttgg gagccataag ggatcacttc
    22381 aactgggagt gggaacttcc caagtttatc gactaattta gaagaatcaa cgatccaaat
    22441 cgtttctttt gagtacgtag cgactatttt ctcgaaaagc aaagcagcgc cgccgccttt
    22501 gatcccttga aaatcagcac tgatttcatc agcaccgtct attgtcaaat cgactacggg
    22561 gacttcatca atactcttta acggtattcc taatttttcc gcttgttctt ttgtcgcatt
    22621 tgaagtagta actcctgtga tacgtaaacc ttcttcacgc atacggcgac caatctcttc
    22681 taccatataa taggcggttg aaccggtccc gagacctacg atcatcccgt cctttacgaa
    22741 tttcgcggct tcaatgccag ctaattgttt taaattcatc tttgcgtcca ccttttttta
    22801 gtttgagcat cgtgctttcc tcactatttt agcgtgattt acggtaaaaa gataccattc
    22861 ttttttattc caataaagct ttttcaaaaa aaattgcatc tcttgttgac atgttttttc
    22921 attcgtgata ttctaacaaa ggtgctttat ctacaggttt ctgtgaagaa acgtgctgac
    22981 tgtttatcaa agcatatttg ataaggatgc agacattgca tttatttttt atcacaaaaa
    23041 aagaaccacc tggatgtgtg gaactagacg caaatcgtgg gaaggaggaa acaaggaatg
    23101 cctacaatta accaattagt acgtaaacct cgtaaatcaa aggtggaaaa atctaattca
    23161 cctgctttga acaaaggata taacagcttt aagaaaactc aaactaacgt aaactcacct
    23221 caaaaacgtg gggtagcaac tcgtgtgggt acaatgacac ctaaaaaacc gaactcagct
    23281 ttacgtaaat atgcacgtgt tcgtttgtca aacttaattg aagttactgc ttatatccca
    23341 gggattggtc ataacttaca agaacacagt gttgtattat tacgcggtgg tcgtgtaaaa
    23401 gacttaccag gggtacgtta ccatatcgtt cgcggtgctt tagatactgc tggtgttaac
    23461 gaccgtaaac aaagccgttc taaatacgga actaaaaaac ctaaagctta attttaatta
    23521 ttaaacaaat gaaacttgct tctactatat agaagtaatt actatgaaca atatttcgga
    23581 aggaggagtt acggatgcca cgtaaaggtc ctgttgcaaa acgtgatgtt ttaccagatc
    23641 ctatttataa ctcaaaatta gtaactcgct tgatcaaccg tgtaatggtt gacggaaaac
    23701 gcgggattgc agctaacatt atctataatg cctttgactt gatcaaagaa tctacaggta
    23761 acgatccatt agaagtgttt gaacaagcaa tgaaaaacgt tcaacctgtt cttgaagtta
    23821 aagcacgtcg tgttgggggt tcaaactacc aagtaccagt tgaagttcgc cctgaacgcc
    23881 gcgttactct aggtcttcgt tgggtagtaa actacgctcg cttgcgcggt gaacacacaa
    23941 tggaagaacg cttagcaaaa gaaatcatgg atgctgcaaa caacacaggt gcttctgtta
    24001 aaaaacgtga agacactcat aaaatggctg atgccaaccg tgcgtttgct cactatcgct
    24061 ggtaagattc tctctgtttg gtcggctttt ataagtcgac ctcagatagt gtccttattt
    24121 ttagcaacta gatagtaaag agaggagaaa caaggagaat gaccagagaa ttttctttac
    24181 aaaacactcg taatatcggt atcatggccc acatcgatgc tggtaaaacg actgctactg
    24241 aacgtatctt gtactatact ggtaaaatcc ataaaattgg tgaaactcat gaaggagctt
    24301 cacaaatgga ctggatggaa caagaacaag aacgtggtat tacgatcact tctgctgcta
    24361 ctacagcaca atggaagggt caccgaatca acatcatcga cacaccagga cacgtagact
    24421 tcacagttga agttgaacgt tcattgcgtg tacttgatgg tgctgtaact gttcttgatg
    24481 cacaatcagg tgttgaacct caaactgaaa cagtttggcg tcaagcaaca acttatggtg
    24541 tacctcgtat cgtatttgca aacaaaatgg acaagatcgg tgctgacttc ttatattcag
    24601 tatcaacttt acatgaccgt ttgcaagcaa acgctcaccc aatccaatta ccaattggtg
    24661 cggaagacga cttcacagga attatcgacc ttgtaacaat gaaggctgaa atgtatacaa
    24721 acgatcttgg aacagagatc gaagaaacag aaattcctga agaataccgc gaattagctg
    24781 aagaatggcg cgaaaaattg gttgaagctg tagctgaaac tgatgaagaa cttactttga
    24841 aatacttaga aggtgaagaa atcactgaag ctgagttaaa agaaggtatc cgccgcgcaa
    24901 cagttaacgt agaattctac ccagtactat gtggttcagc atttaagaac aaaggggttc
    24961 aattactatt ggacgctgtt cttgattact tgccatcacc gttagatatc ccagctatca
    25021 aagggattga ccctaaaact gatgaagaag tagaacgtcc tgcagacgac agcgctcctt
    25081 tctcagcttt agcatttaaa gttatgactg acccattcgt aggtcgttta actttcttcc
    25141 gtgtgtactc tggtgtttta caatcaggtt catacgtaca aaacgcaaca aaaggcaaac
    25201 gtgaacgtgt tggtcgtatc ctacagatgc atgccaactc tcgttcagaa atctctgaag
    25261 tttacgctgg agatatcgct gctgctgttg gtttgaaaga cacaactact ggggacacac
    25321 tatgtgatga aaagagtcta gttattcttg aatccatgga attcccagaa ccagttatcc
    25381 aagtcgctat tgaacctaaa tcaaaagctg accaagataa aatgggtgtg gctttacaaa
    25441 aactatccga agaagatcct actttccgtg ctgaaacaaa cgtggaaaca ggcgaaacaa
    25501 tcatcgctgg tatgggtgaa cttcacttag atatcatcgt tgaccgtatg cgtcgtgaat
    25561 tcaaagttga agctaacgta ggtgcgcctc aagtatctta ccgtgaaact ttccgtgctg
    25621 gcactcaagc cgaaggtaag tttgtacgtc agtctggtgg taaaggtcaa tacggtcacg
    25681 tatggattga atttacacca aatgaagaag gtgctggttt cgaatttgaa aatgcgatcg
    25741 tcggtggggt tgttcctcgt gaatacattc cagctgttga aactggtttg aaagatgcta
    25801 tggaaaatgg tgtcttagct ggctatccat tagtagatat caaagctaaa ctttacgatg
    25861 gttcatacca tgatgtcgat tcaaatgaaa ctgccttccg tgttgctgct tctatggcat
    25921 tacgtgcagc agctaagaaa gctaatccag taatccttga accaatcatg gctgtagaag
    25981 ttgttatccc tgaagattat ttaggtgatg ttatgggtca cgtaactgct cgtcgtggac
    26041 gtgttgaagg tatggaagct cgcgctggcg gccaacaagt tgttcgtgcg atggttccac
    26101 ttgctgaaat gtttggttat gctacaactc ttcgttctgc tactcaaggt cgtggtacat
    26161 ttacaatgac atttgaccac tacgaagatg taccaaaatc agtacaagaa gaaatcatta
    26221 agaaaaacgg cggaaaagct gaataatttc ttaaaaccgg atagtcctgc aaagagatta
    26281 tccaaagaga ctatattttt aatatagttt cgtgtaaaat aattatcgat gatatgggca
    26341 tgtggtgtac cttgtgtaca tcatatacct atcagacaaa taaaaaatat attgtatttt
    26401 taggaggaca ttttaaaatg gcaaaagaaa aatttgaccg ttctaaacca cacgttaaca
    26461 ttggtactat cggacacgtt gaccatggta aaactacatt aacagctgca atcacaactg
    26521 tactatctaa gaaaaacggc ggccaagcta tggcttacga tcaaatcgat ggtgctccag
    26581 aagaacgcga acgcggaatc acaatctcaa cagctcacgt tgagtatgaa acagatacac
    26641 gccactacgc tcacgttgac tgcccaggac acgcggatta cgttaaaaac atgatcactg
    26701 gtgctgctca aatggacgga gctatcttgg tagtttctgc tgctgacggc ccaatgcctc
    26761 aaactcgtga acacatccta ttgtctcgtc aagttggtgt tccttacatc gttgtattct
    26821 tgaacaaagt agacatggtt gatgacgaag aattactaga attagttgaa atggaagttc
    26881 gtgacctatt aacagaatac gaattccctg gtgacgatgt tcctgtagtt gctggatcag
    26941 ctttgaaagc tctagaaggc gacgcttcat acgaagaaaa aattcttgaa ttaatggctg
    27001 cagttgacga atacatccca actccagaac gtgacaacga caaaccattc atgatgccag
    27061 ttgaagacgt gttctcaatt actggacgtg gtactgttgc tacaggtcgt gttgaacgtg
    27121 gacaagttcg cgttggtgac gaagttgaag ttgttggtat tgctgaagaa acttcaaaaa
    27181 caacagttac tggtgttgaa atgttccgta aattgttaga ctacgctgaa gctggagaca
    27241 acattggtgc tttactacgt ggtgttgcac gtgaagacat ccaacgtgga caagttttag
    27301 ctaaaccagg tacaatcaca cctcatacaa aattctctgc agaagtatac gtgttgacaa
    27361 aagaagaagg tggacgtcat actccattct tcactaacta ccgtccacaa ttctacttcc
    27421 gtacaactga cgtaacaggt gttgttgaat taccagaagg aactgaaatg gttatgcctg
    27481 gcgacaacgt aacaatggaa gttgaattaa tccacccaat cgctatcgaa aacggtacta
    27541 aattctcaat ccgtgaaggc ggacgtacag ttggtgccgg tgtcgttaca gaaatcaaag
    27601 cttaattcac ttaagtttta aagtaaagca acatttattc ggacgtaatc gctcggaacg
    27661 aaggtcttag gatctttgtt ccgagctttt ttatttattg caggatcagg aaaaaagaca
    27721 ggagtattat aaatagaaga aacacgaaat aaaagagtca gtattttcta aatgaaactt
    27781 tatgaaaaca aaatgttttt tacaagaaaa agccttgctt tgtgagttta tatatattat
    27841 aatgacaaag gtgcgtttac tttagtgaat tgcacagacg taatcagaat gatttcgtcg
    27901 gctaagaaac gagaggttgc gacacgcccg gacgctttgc cacgaacgag cgtgacggaa
    27961 attttcgtgg agctatgtct acttttcaaa ataggcgaag gagggaacaa gatggcaaaa
    28021 caaaaaattc gtatccgttt aaaagcgtat gaacaccgta ttttagatca atcagcggat
    28081 aaaatcgtgg aaactgcaaa aagaactgga gctagcgtgt ctggtccaat tccattacca
    28141 actgaacgca gtctttacac aatcatccgt gcgactcata aatacaaaga ttcacgcgaa
    28201 caattcgaaa tgcgtacaca caaacgtctt atcgacattg tgaacccaac accaaaaact
    28261 gttgatgctt tgatgaagct agacttacca tctggtgtaa acatcgaaat caaattataa
    28321 aactaaaaca aaaataatgg aggtgtactc atgaccaaag gaatcttagg gaaaaaagtg
    28381 ggaatgacac aaatcttcac tgaatctggt gaattaattc cagtaactgt tgtagaagct
    28441 actccaaacg tagtactaca agtgaaaaca atggaaacgg atggctacga agctattcaa
    28501 gttggttacc aagatatgcg tgaagtttta tcaaacaaac ctgcgaaagg tcatgttgca
    28561 aaagcaaaca cggctcctaa gcgcttcatt cgtgaattca aaaatgttga gctaggagaa
    28621 tatgaagtag gaaaagaaat tacagtagat gttttccaag caggagacat tattgatgta
    28681 acaggtacta cgaaaggtaa aggattccaa ggggttatca aacgccacgg acaaagccgc
    28741 ggaccaatgg ctcacggttc tcgttaccat cgtcgtcctg ggtcaatggg tccagttgca
    28801 cctaaccgtg tatttaaaaa caaacgtctt gctggccgta tgggtggcaa ccgcgtaact
    28861 atccaaaact tggaagttgt acgtgtcgat gcagaaagaa atgttatctt gatcaaaggt
    28921 aatgttcctg gagctaaaaa atctttaatt actatcaaat cagctgtgaa agctaaataa
    28981 ttgtaggaag aaaggaggaa ctaaggaatg ccgaatgtag cattattcaa acaagatgga
    29041 agccaaaacg gtgaaatcac acttaacgaa gaaatcttcg gaattgaacc taatgaaagt
    29101 gttgtttacg atgcaatcgt aatgcaacgt gcgtcattaa gacaaggaac acacgcggtt
    29161 aaaaaccgta gcgctgttcg cggcggcgga cgtaaaccat ggcgtcaaaa aggaacaggt
    29221 cgtgcacgtc aaggatctat ccgctcacca caatggcgtg gaggtggcgt agtctttgga
    29281 ccaacaccac gttcttacag ctacaaactt cctaaaaaag ttcgtcgttt agcaatgaaa
    29341 tctgtattgt cagaaaaagt tgctgaaaac aacttggtag caatcgaagg cttgaacttc
    29401 gacgcaccaa aaacaaaaga attcaaacaa gttcttgcta acttatctat cgactctaaa
    29461 gtattggttg ttctagaaaa aggtaacgac tttgctgctt tatcagcacg taacttacca
    29521 aacgtttctg tagtaacttc tgataatgtg agtgtgctag acgttgtatc aaatacaaaa
    29581 gtgttagcaa cacaaactgc tcttactcaa attgaggagg tgcttgcata atgaacttac
    29641 tagacgtaat caaacgccca gtgatcactg aaaaatcaat gcttgccatg gatgacaaga
    29701 aatacacttt tgaagtggac actcgcgcaa acaaaacttt agtaaaacaa gcggtagaag
    29761 ctgcttttga cgttaaagtc aaaaacgtga acatcgtgaa cgtacgtgcg aaattcaaac
    29821 gtatgggcaa atatgcagga tatacaaaaa aacgtcgcaa agcgatcgtt acattgactg
    29881 aagattcaaa agaaatccaa ttattcgaag ctgctgaata agcactaata gataactcat
    29941 taaattagga gggaaaaacg tggcgattaa aaagtacaaa cctacctcaa atggtcgtcg
    30001 taacatgaca ggttctgatt ttgctgaaat cacaacatca acacctgaaa aatcattgtt
    30061 acaaccattg aaaaaccatg ctggacgtaa caacaacggc cgcatcactg tacgtcatca
    30121 aggtggcgga cacaaacgcc aatatcgcgt gatcgacttc aaacgtaaca aagataacgt
    30181 tgttgcaact gttcaaacga tcgaatatga tccaaaccgt tcagctaaca tcgcgttagt
    30241 acactatgaa gatggagtga aagcttacat cttagcacct aaaggactag aagtgggtat
    30301 gaaacttgtt tcaggtccag aagcagatat caaagtcgga aatgctcttc cattagaaaa
    30361 cattccagtt ggtactgtta tccacaacat tgaaatgaaa cctggtaaag gtggacaatt
    30421 gatccgttct gctggtacaa gtgctcaagt acttggtaaa gaaggcaaat acgtattgat
    30481 ccgcttgaac tctggtgaag ttcgtatgat cttagctgca tgccgtgcta caatcggttc
    30541 tgtaggtaac gaacaacacg aattaatcaa catcggtaaa gctggacgct ctcgttggat
    30601 gcgtaaacgc ccaactgtac gtggtagcgt aatgaaccct aatgatcacc cacacggtgg
    30661 tggtgaaggt aaagctccta tcggacgtaa agcaccagtt tctccttggg gtcaaccagc
    30721 tcttggttac aaaactcgta acaaaaaagc taaatcagac aaacttattg ttcgtcgtcg
    30781 caaaaataaa taattacttt gaccatgtgt cgcacatttt gagaggaggt tcaccatggg
    30841 tcgtagttta aagaaaggac cttttgtcga tgagcattta atgaaaaaag ttgaagctca
    30901 agcaggggtt gaaaagaaaa aagttatcaa aacttggtca cgccgttcaa cagttttccc
    30961 aagttttgtt ggatatacca tcgcagttta tgatggacgt aaacatgtac cagtttacat
    31021 ccaagaagac atggtaggac acaaattagg tgaatttgca cctacaagaa cttatcgtgg
    31081 acatgctgct gacgataaga aaacgaaacg ctaatttgag gggaggataa atccaaatgg
    31141 cagaacaaat cacatcagct aaagcaactg caaaaacagt tcgcacttca cctcgaaaag
    31201 cccgtttagt aattgacctt atcagaggta aaagtgtcgc agatgcaatt tcaatcttga
    31261 aattcacacc aaacaaatct gctggaatca ttgaaaaagt attgatgtca gcagttgcga
    31321 atgcagaaaa taactttgac ttagatgttg aaaacttggt agtaagcgaa gcttttgtta
    31381 acgaaggacc tacaatgaaa cgtttccgtc ctcgtgcaaa aggatcagct tctccaatca
    31441 acaaacgtac aagtcatatc acggtagtcg tatcagaaaa ataaggaggg ataatcagtg
    31501 ggtcaaaaag tacatccaat tggaatgcgt gtaggcatca tccgcgactg ggatgctaaa
    31561 tggtatgctg aaaaagagta tgctgagttc ttacacgaag atttaagaat ccgtaaattt
    31621 atttcaacta aacttgctga tgctgctgta tcaacagttg aaatcgaacg cgcaacaggt
    31681 cgcgtaaaca tttcaattca cacagctaaa ccaggtatgg ttattggtaa aggcggttct
    31741 gaagtcgaaa gcctaagaaa agaattaaac aaattaactg gtaaaagaat ccatatcaat
    31801 atcgtggaaa tcaaaaaacc agatctagat gcaaaattag taggtgaagg aatcgcacgt
    31861 caattagaaa accgtgttgc tttccgtcgc gctcaaaaac aagccatcca acgctcaatg
    31921 cgtgctggcg ctcaagggat caaaactcaa gtatctggtc gtctaaacgg agcagatatc
    31981 gctcgttcag aaggatactc agaaggaact gttcctcttc atactttacg tgcggacatt
    32041 gattacgcat gggaagaagc agatacaaca tacggaaaac taggagttaa agtgtggatc
    32101 tatcgtggag aaattcttcc aactaaaaaa aacactgaga aaggagggaa ataatcatgt
    32161 tagtacctaa acgtgtaaaa caccgtcgtg aattccgcgg aaaaatgcgc ggggaagcta
    32221 aaggcggaaa agaagtagca ttcggtgaat acggtttgca agctgttgat tcacattgga
    32281 tcacaaaccg ccaaatcgaa gctgctcgta tcgcaatgac tcgttacatg aaacgtggtg
    32341 ggaaagtatg gattaaaatt ttccctcaca aatcttatac tgccaaagca attggggtac
    32401 gtatgggttc tggtaaaggg gcacctgaag gatgggttgc accagtaaaa cgtggtaaaa
    32461 tcatgtttga aatcgcaggc gttcctgaag aagtagctcg tgaagcgtta cgtctagctt
    32521 ctcacaaatt accaatgaaa actaagatcg taaaacgtga ggaaatgggt ggtgaatcga
    32581 atgaaggtta aagaaatcag agaattaacc actgccgaaa tgcttgatca agaaaaacaa
    32641 ttaaaagaag aattgtttaa tcttagattc caattagcaa caggtcaatt agaaaacact
    32701 gcacgtatta aagaagtacg taaatcgatt gcacgcatca aaactgtgtt gcgtgaacaa
    32761 gctaagtaat tatggaagga ggccattaag cgtatgactg aaggaagaaa tcaacgcaaa
    32821 gtttatcaag gtcgcgtggt ttcagacaaa atggataaaa caattaccgt tgctgtcgaa
    32881 acaaagaaaa accaccctat ctatggcaaa cgcatgaact attctaagaa atacaaagtg
    32941 catgatgaaa acaacacagc aaaagttggc gacatcgtga gaattatgga aactcgtcca
    33001 ttatcagcta caaaacgttt ccgtttgttg gaagtagtcg aagaagcagt tattatctaa
    33061 taacacgaga ttttcctata tagattgaat gcaaaatctg aaaggaggat acacaacgtg
    33121 atccaagcag aaagtcgttt aaaagtcgct gataactcag gcgcacgtga aattttgacg
    33181 atcaaagtcc ttggtggatc tggtcgtaaa actgctaaca tcggtgatgt gatcgttgct
    33241 acggttaaac aagcaacgcc aggtggggtt gtcaaaaaag gtgacgtcgt taaagccgtt
    33301 atcgttcgaa caaaatcagg agctcgtcgt acagacggtt catatattaa atttgatgaa
    33361 aatgctgcag tgattattcg tgatgataaa agccctcgtg gaacacgtat ctttgggcca
    33421 gttgcacgtg aactacgtga aaacaacttc atgaagatcg tttcactagc accagaagtt
    33481 ttataatctt gagacactta tcaaaggagg tgcgaaacag ttatgtttgt taaaaaaggc
    33541 gataaagtta aagttatcac tggaaaagac aaaaacaaag aaggcgttgt actagcagcg
    33601 ttcccgaaaa aagataaagt tattgtcgaa ggtgtaaatg tcgttaaaaa acaccaaaaa
    33661 ccttctcaag ctgctcctca aggtggaatt gttgagatgg aagcgccaat tcatgtttct
    33721 aacgtgatgg tggttgactc tactggtgta gctggccgag ttggctacaa agaagtagac
    33781 ggcaaaaaag tccgtgtttc taaaaaaacc ggtgaagttt tagataaata aaagtttaag
    33841 gaaggagggt cttactgaat gaaccgccta aaagaaaaat atcttaaaga agtaactcca
    33901 tcattgatgg aaaaatttaa ctatagctct gttatgcaaa cacctaaagt tgaaaaaatc
    33961 gtcatcaaca tgggtgttgg tgatgcggta tcaaacgcta aaaacttaga taaagcagtt
    34021 gaagaattaa ctttgatcac tggtcaaaaa ccaatgatta caaaagctaa aaaatcaatc
    34081 gctggattcc gtttacgtga aggaatgcca attggtgcaa aagttacatt gcgcggagaa
    34141 agaatgtacg aattcttaga caaattggtt tctgtttcac taccacgtgt acgtgacttc
    34201 catggtgtaa gtaaaaaagc ctttgacggt cgtggaaact atactttagg tattaaagaa
    34261 caattgatct tcccagaagt agattatgat ttagtagata aagtacgtgg tatggacatc
    34321 gttattgtaa caacagcaaa ctctgatgaa gaatctcgtg agttgttgac acaattaggc
    34381 atgccattcc aaaaataatt aaaggaggcg aactacgtgg ctaaaaaatc aatgattgct
    34441 aaaaacaaac gtcctgcaaa attttcaaca caagcttata ctcgttgcga acgttgcgga
    34501 cgtccacatt cagtttatcg taagttccat ctttgccgta tttgcttccg cgaacttgcc
    34561 tataaaggtc aaattcccgg cgtgaagaaa gctagctggt aaaaagtaaa ttcgcataag
    34621 aaggaggtaa aagatcaatg gtcatgacag atccaattgc agattttcta actcgtatcc
    34681 gtaacgcaaa catggttaaa catgagtctt tagaagtacc tgcatcaaaa attaaacgtg
    34741 acatcgctga aatcttgaaa cgtgaaggtt tcgttcgtga tgttgaatac atcgaagatg
    34801 acaaacaagg cgtgatccgt gttttcctaa aatttggtaa aaacggcgaa cgtgttatca
    34861 caaacttaaa acgtatctct aaaccaggtt tgcgtgctta tgtcaaagct gacgaagtac
    34921 caaaagtatt gaacggttta ggtatcgcaa tcatttcgac ttctgaaggt gttgtcactg
    34981 ataaagaagc tcgtgaaaaa aatatcggcg gcgaagttat cgcttacgta tggtaatcaa
    35041 gtagaatcta caaggaggtg tctttaagtg agccgtatcg gaaataaagt cgttgttatc
    35101 cctgaaggag taacagttac tcaagacgga aacaacgtta cagttaaagg accaaaaggt
    35161 gaattgactc gtactttttc agcagatatc aaaatgaata tcgaaggaaa cgaagtaact
    35221 ttcactcgtc caaacgacag taaagaaatg aaaacaattc atggaacaac tcgtgcgaac
    35281 ttcaacaaca tggttgtagg tgtcagcgaa ggtttccaaa aagcgttaga actaatcggg
    35341 gttgggtacc gtgcacaatt acaaggatca aaacttgtat tgaacgttgg ttactcacat
    35401 ccagtagaaa tcacaccacc agctggagta actgttgaag taccagctaa cacacaagta
    35461 attgttaaag gtgcgaacaa agaagaagtt ggcgaattag ctgcaaacat tcgtggaact
    35521 cgtcctccag aaccttataa aggtaaaggt attcgttatg ttggcgaata tgtacgccgt
    35581 aaagaaggta aaactggtaa ataatcgttt agaaaaattg catagaagcc agcagcagga
    35641 aaaaggcatt tggctgaagt ttaccttcaa aaaaatacat ttttacttgt gccgcagctt
    35701 ctatttttcg aaacgtattt actgcttgaa atagtagtac aataaaaata aagaggtgac
    35761 aattgtgatt acaaaaccag ataagaacaa aacacgtcaa aagagacatc gtcgtgtacg
    35821 taacaatatc tctggtactg ctgagcgccc acgcttgaac gtttttcgtt ctaacaaaaa
    35881 catctacgct caaattattg atgacgtagc gggtgtaacg ctagcaagtg cctctacctt
    35941 ggataaagaa atttcaggtg gaacaaaaac agaagcagca caagctgtcg gtaaactagt
    36001 tgctgaacgt gcagctgaaa aaggcattaa agtagtagtc tttgaccgtg gtggatacct
    36061 ttaccatggc cgtgtgcaag ctttagctga agctgctcgc gaaaatggac tagaatttta
    36121 gaagaaggag gaataccatt catggtttat atcgatccaa aacatttgga attagaagac
    36181 cgcgttgttg caattaaccg tgtaacaaaa gttgttaaag gtggacgtcg tctacgtttt
    36241 gctgctttag ttgttgtcgg tgacaaaaac ggacacgtag ggtttggtac aggtaaagct
    36301 caagaagttc ctgaagcaat ccgtaaagca attgaagatg cgaagaaaaa cttagttgaa
    36361 gtacctatgg ttggttcaac aatcccacac gaagtaatcg gtgcttttag tggtggtaga
    36421 atcctaatga aacctgctgt agaaggttct ggggttgctg ctggtggacc agttcgtgcc
    36481 gtattagaat tagctggggt agcagatatc acttctaaat ctttaggctc aaatacacca
    36541 atcaacgttg ttcgcgcaac agttgaaggt ttgaaacaat tgaaacgtgc agaagaagtt
    36601 gcagaacttc gcggcaaatc agttgaagaa ttaatcggat aaggaggaca acaataatgg
    36661 ctgaattaaa agttacttta aaacgcagta ttatcggacg tcctcaaaac caacgcgata
    36721 cagttaaagc gttaggtctt acaaagatta acagttcagt ggttaagcct tctaatgaag
    36781 caattaaagg catgatcaat actgtttctc acctagtgga cgttgaagaa atttaattta
    36841 gaaaaatcat aactagaaca ttaaggaggt gccaaaccta tgaaacttca tgaattaaaa
    36901 cctgctgaag gttcacgcca agtacgtaac cgtgttggtc gtggtacttc atctggtaac
    36961 ggaaaaactg ccggacgcgg acaaaaagga caaaaagctc gttcaggcgg cggtgtgcgt
    37021 ttaggatttg aagggggaca aacaccattg ttccgtcgtt taccaaaacg aggattcaca
    37081 aacgtgaacc gtaaagacta tgcagtcatt aatcttgatg tattgaaccg ctttgaagac
    37141 ggagctgaag taactcctgt tgctttagtt gaagctggaa tcgtgaaaaa cgaaaaagct
    37201 ggaatcaaag tattagctaa tggggaacta aacaagaaat taactgtgaa agcagcaaaa
    37261 ttctctaaag cagctcaaga agcaattgaa gcggctggtg gatcaatcga ggtgatctaa
    37321 tgtttagact attaaaagat gcttttaaag taaaagacat tagatcgaaa atcctattta
    37381 ctgtatttgt gttgtttgta tttcgcctag gtgcacatat tactgtacca ggcgtaaatg
    37441 ctgcttcatt gcaaaacctt tcttctttgc catttttcaa catgttgaac ttggtaagtg
    37501 gtagtgcgat gcaaaacttt tccattttct caatgggggt ttcaccatac attactgctt
    37561 ctatcgtcat tcagcttctt caaatggaca tcgtgcctcg atttgttgaa tggtcgaaac
    37621 aaggggaagt tggtcgtaaa aaactgaatc aagcgactcg atatctgaca ttagtattgg
    37681 catttatcca atctgtcggt atcactgctg gattcaatca atacgctcaa ttaggtttcg
    37741 tgaataatcc taacgtagca acttacgtaa cgatcggttt gattctaaca gccggtacga
    37801 tgtttgttac ttggctagga gaacagatca cagaaaaagg tatcggaaat ggcgtatcaa
    37861 tgatcatttt tgcaggtatc atttctagat tgcctgaaag tatcagagaa atttatgaag
    37921 attactttat aaatattgat ccttcagaca tatggaaatc tgctattttt gtggccattt
    37981 tactagtagc ggtcttgttg atcgtgacat tcgtaacatt tttccaacaa gctgaacgca
    38041 agatcccgat ccaatatacg aaacgcgtag ctggcgcgcc tacaagcagc tatctgccgt
    38101 tgaaagtaaa tgcagctgga gttattccag ttatctttgc tagttcgttt attgctacgc
    38161 caaatgctgt tttacaagca ttgagcggta gattcggttc tgaaaactgg tacaaagtga
    38221 tgatggagat ctttaactat aatacagcac ctggtgctac gatttatacg attctgatcg
    38281 ttgccttcac tttcttctac gcctttgttc aagtaaaccc tgagaaatta gcggaaaact
    38341 tgcaaaagca aggaagttat ataccaagcg tacgccctgg taaggggact gaagagtatg
    38401 tctcaagatt actgatgcgt ttaagtacag ttggtgcagt cttccttggt ttagtggcat
    38461 tgcttccaat cattgctcaa atggtttggg atcttccaca atcaatcgga ttaggcggta
    38521 caagcttact gatcgttatc ggtgtagctt tagaaactgc gaaacagtta gaaggcttaa
    38581 tgctgaaacg taaatatgtt ggattcattg agtaagaaag cgtgttgagg tttcccctca
    38641 acacacgttc tcacacattt attaggaggg aaaacaatga acctcatttt aatggggctg
    38701 ccaggcgcag gtaaaggaac acaagctgaa aaaatcattg atgcttatgg tattccgcac
    38761 atctcaacag gagatatgtt ccgcgcagcg atgcaaaacg aaacagctct tggcctggaa
    38821 gcaaagtctt atatggataa aggtgcatta gttcctgatg aagtaacaaa tggaatcgtg
    38881 aaagagcgac tagctgaacc agatacagag aaaggcttct tattagatgg ctttccacgt
    38941 acgctagatc aagcaaaagc tctggacgca atgctaaaag atttgaataa aaaaattgat
    39001 gctgtcatcg atatccatgt tggtgaagaa atcttggtcg aacgtctagc cggccgattt
    39061 atctgcagca attgtggagc aacttaccat aaaattttca accctacaaa agttgaagac
    39121 acatgtgatc gttgcggtgg gcatgaattc tatcaaagag aagatgataa acctgagacg
    39181 gttaaaaatc gtctggctat caacatcaaa aatagtgaac caatcttggc ttattataaa
    39241 gaacaaggtt tgctaaacac tattgatggt gctcgtgaaa tcgatgctgt atttgcagat
    39301 gtacaaaaaa tcattgaaaa atagcacgat tgcccgtagc cgacttgcat tatgcagtta
    39361 ctcttgtcaa gttcaataaa ttatgataga atatgtcagt ccgacttcta gagaatcctc
    39421 tagatgatcc attttgagaa ttttgaggtg ggaactgcag tttccgccat tttatcgtgt
    39481 gaaaatgcga aacaagtaca aggaggtact gtgcgtggca aaagaagata tgattgaagt
    39541 cgaaggtaca gtcgtcgaaa ctttgccgaa tgcaatgttt aaagttgaac tagaaaacgg
    39601 acaccaagtt cttgctactg tttcaggtaa aattcgtatg cattacatcc gtatcttacc
    39661 tggtgacaaa gtaacggttg aattgtctcc atacgatctt aaccgtggcc gtattactta
    39721 tcgctttaaa taattgtact ccgtaaaact acaggaggta ttatcatgaa agtaagacca
    39781 tcagtaaaac caatgtgcga acattgtaaa gtaattcgtc gtaaaggacg cgttatggtg
    39841 atttgcccag caaatccaaa acataaacaa cgtcaaggat aattggaggt gctataaata
    39901 tggctcgtat tgcaggagta gatattcctc gtgataaacg tgtagtagtt tcacttactt
    39961 atatctatgg tattggtaac actactgcta aaaagatctt agctgatgct ggtgtatctg
    40021 aagatgttcg cgttcgtgaa ttaacgaatg aacaaacaga tgctatccgt gcagaagtag
    40081 ataaattaaa agttgaaggg gatcttcgtc gtgaagtgaa cttgaacatc aaacgtttga
    40141 tggaaatcgg ttcttaccgt ggaatccgtc atcgccgtgg attgcctgtt cgtggacaaa
    40201 acacgaaaaa caatgcacgt actcgtaaag gcccagctcg cgctatcgct ggcaagaaaa
    40261 aataattctt aagtgaagga ggttaaaact tcatggcagc aaaaaaagtg aatcgtaaac
    40321 gccgtgtgaa gaagaatata gaagcaggta ttgcacatat ccattctaca ttcaataaca
    40381 ctatcatcat gatcactgat actcacggaa atgcattagc gtggtcatca gctggttcat
    40441 taggtttcaa aggaagcaaa aaatcaactc cttttgccgc tcaaatggca gcagaaactg
    40501 ctacaaaagc agcaatggaa cacggattaa agactgttga agtaacagtt aaaggacctg
    40561 gttctggacg tgaagcagca attcgttcat tacaagctac aggtttagaa gtgactgcaa
    40621 ttcgtgacgt gactccagtt cctcataatg gtgcccgccc tccaaaacgc cgtcgtgttt
    40681 aatgagtgcc gctcattttg agttttatca gtaacgtcat tgaacaagac acttttcgtt
    40741 ttgaaagggg taaagataag aatgattgag ttcgaaaaac caagaatcgc aaaaattgat
    40801 gaagaaaaag attatggcaa gttcatcgtt gaacctcttg aaagagggta tgggactact
    40861 ttagggaatt ccctacgtcg tattttatta tcttctttgc caggtgctgc catcactaat
    40921 attcaaatcg atggtgtact gcatgaattc tctaccatca aaggtgttag agaagacgtc
    40981 actcaaatca tcttgaacat taaaggctta gcgctgaaaa tgtatggcca agaagaaaaa
    41041 acccttgaaa tcgatattac cggacctgct acagtaactg ctggcgatat tatcgttgat
    41101 agtgatgtag aaattcttaa caaagatatg tacatctgta gtgtctctga aggagctacg
    41161 ttccatgctc gcttaacagt gaaacctgga cgtggttatg ttcaagcaga tgaaaataaa
    41221 aaagaagata tgccaattgg cgttcttcca gtcgattcaa tctacacacc tgttcgtcgt
    41281 gtgaactatc aagttgagaa cacacgtgtt ggacaccgtg aagattttga taaattgacg
    41341 atggagattt ggacagatgg ttcaattgaa ccgttggaag caatgagttt gtctgcgaaa
    41401 atcatgactg aacatttgga tatttttgtg aacttaactg atgaagcaaa aaatgctgaa
    41461 atcatggtcg aaaaagaaga aacacaaaaa gaaaaaatgt tggaaatgac gatcgaagag
    41521 ttagacttgt ctgttcgctc ttacaattgt ttgaaacgtg caggaatcaa tactgtacaa
    41581 gaacttacaa ataaatctga gcctgaaatg atcaaagtcc gtaacttagg acgtaaatca
    41641 ttagaagaag ttaaagcaaa attgcacgac ttaggtttag gtttacgtaa agaagattaa
    41701 aaccttttac gaaggaggga aaccaacgtg agttatcgta aactaggacg cacatctagc
    41761 caacgtaaag cgatgttgcg tgatttaaca actgatttat taatcaacga acgtattgtg
    41821 actactgaag ctcgtgcgaa agaagttcgc tcaactgcag aaaaaatgat cactttaggt
    41881 aaacgcggag atttgcatgc acgtcgtcaa gctgcggctt tcgtacgtaa tgaagttgcc
    41941 agcgtgcgtg aagaaaatga ggacatcgtc attgaatcag ctttacaaaa actgttcaac
    42001 gatattgcac ctcgttacgc agaacgtcaa ggcggataca cacgcatttt gaagacagaa
    42061 ccaagacgcg gtgatggtgc accaatggtt gtcttagaac ttgtctaaaa tcacactaat
    42121 aacatcactt tatagatgat tcttttagag tgttatgatg ttggaagata tttttccgag
    42181 tctagctcag cgctgctcct caagcgaata tcgcttgggg agtaagcact catcataaag
    42241 tgttttttta taccttcaat tacctgtcat gacagatatg tttgaaggca taaaaaaaca
    42301 ttttaagcga gaaataattg aaaattttgt ctttttttga tagtgttagt aggaacttta
    42361 ggatgaagaa ggagtaatca tggagccaat tattgaatta gaaaagataa attataaata
    42421 ccagccagat gatcttcgcc ccgcattgaa agatgtctcc tttacaattg ataaaggtga
    42481 gtggatcgct attattggac acaatggttc aggaaaatcg acacttgcca aaacgatcaa
    42541 tggattgctt ctgccagaat cagggatcgt gaaagtggga aatcaaatat tggatgaaga
    42601 aaatatttgg acgatccgac agatggttgg aatggttttt caaaatccag acaatcagtt
    42661 tgttggttcg acagtagaag atgacgttgc ttttggtctt gaaaatcaag ggattcccag
    42721 agaagaaatg ctagtgcgtg tgaaagatgc acttgagaaa gttagaatgg ctgagtttgc
    42781 ttcacgcgag cctgctcgtt tatcaggagg gcaaaaacaa cgtgttgcca ttgccggcgt
    42841 ggttgcctta cgacctgata tcattatttt agatgaagca accagtatgc tagatccaga
    42901 aggtcgtgaa gaagtgatct ctacgatcaa gaagatcaaa gaagaaagtc aattgaccgt
    42961 tatatctatc actcatgata ttgatgaagc agctaatgcc aatcggatac tagtgatgag
    43021 acaaggcgaa cttgtccgtg aaggtacacc aaaagaaatt ttttctgctg gtcctgaact
    43081 gatcgactta ggtctagatc taccatttcc tgaaaaactg aagagcgcac tgaaagaacg
    43141 tggggttgat gtgcccagtg aatatatgac agaagaaagg atggtggatt ggttatggac
    43201 atccgttttg aacaagtaga cttcacttat cagccgaata caccatttga acaaagagcc
    43261 ttatttgaca tcaatatgac gatcaaagaa aacagttata ctgcattagt agggcatacc
    43321 ggaagcggga aatcaacctt acttcagcat ttgaatgcgc ttgttaagcc aacaagcggt
    43381 acagtccata ttggagaacg agatattcag ccggatacgg ataataagaa tttaaagccc
    43441 atccgaaaaa aagttggcat cgtctttcag tttccagaag cacagctatt cgaagaaacg
    43501 gtagcaaaag acattgcttt cggtcctaaa aactttggtg tcagcgaaga agaagcacta
    43561 gtcctagcaa aagaaacatt agaacaagtt gggctggatg aaagctattt ggaacgttcg
    43621 ccatttgaac tttcaggagg gcaaatgcgt agagtagcaa tcgctggtgt gcttgccatg
    43681 aggccggaag tacttgtatt ggatgaacca acggcaggac ttgatcccca gggcagaaaa
    43741 gaaatgatgg agatgttttg gcgtcttcat aaggaacagc agatcactat tgtattagtt
    43801 acgcatttaa tggatgatgt agccaatttt gcagattatg tctatgtgtt ggaaaaggga
    43861 cgaatcgtta atagcgggga gccgcaagag gtctttcaaa acattgaatg gctaaaagaa
    43921 aaacaactag gcgtacctac tgccaccgag tttgcagaag aattaatggc taaagggatg
    43981 aactttgcta ccttgccttt gacagctgaa gaattagcag acgctatcgt ccattatgct
    44041 ggaggaaatg cccatgatga ataaattgat atttggtcgt tatatgcctg gagattcctt
    44101 gatccatcga ttggatcccc gagctaaatt attagccagt ttttatttta tcggaatcat
    44161 ttttttagca aataattggg aaacgtacct tttgttggca tttttcacat tactttctgt
    44221 cttcttgtca aaagtagatt tgaaattttt tatccgaggc attcgcccat tgatctggct
    44281 aatcctcttc actgtagctt tgcaggtctt gtttacaagt ggcggagaaa cttattggag
    44341 ttggggtgtt ttccatatca ctgaatttgg tgtgatgaac ggcctcttta ttttctgtcg
    44401 attcgtgttg atcattttta tgtcgactct actcacgctt actacaccgc ctttggaatt
    44461 atcggatgcg attgagtact tgcttcgtcc attgaaagcc gtacggttcc cagtccacga
    44521 aatttcattg atgctgtcga ttgctcttcg ttttgtgcct acattgatgg acgaaacaga
    44581 aaagatcatg aacgctcagc gcgctcgcgg tgttgatttt ggtgaaggaa acctagttca
    44641 gaaaatgaaa gcagtcattc ctttgctgat tcctttattt gtcagcagct tcaaccgtgc
    44701 ggaagatctt gcgactgcta tggaagcaag aggctacaaa ggtggggaag gaagaacgaa
    44761 atatcgtgta ttgcattggc ataatagaga cacgatggtt atcattgctt ttgtattatt
    44821 aactgtgcta ctgatctttt taagaggata aacgttcagt gggccgctat ctattctttt
    44881 cttgctgatc tgtttgcaag aaaggatgca agcagtgtat gacaagaaat attagggtct
    44941 gtggcagact aaggtcacag gcctctttct tttatctgaa aagaaagaac gaaaaaattt
    45001 tatagaaaaa tcattattcc agataacctt ttaggaacaa aggagagaaa aacatggtac
    45061 gttacaaagc aatcattgcg tacgacggaa caaattttaa tggattccaa aaacagccaa
    45121 atgggcgtac tgttcaagaa gaagtagaaa aaacattaca aaaaatggca aatggaaaag
    45181 aaatcacggt ctttggttcg ggaaggacag atgccggagt acatgcgatc gggcaagtaa
    45241 tccattttga ttatccagag gagcgtccgt tagaacgaat gcgtttcgcg ctagataccc
    45301 agtcaccaga ggatattgct gtaagacaag tagaaattgt ttcagaagac ttccacgcaa
    45361 gatatttggt caaagaaaag acgtatcaat ttcgagtgga tatagggaaa ccacgcagtc
    45421 cttttcgacg ccattatgca agttattttc cttacccact tgatttggaa aaaattcagc
    45481 gggcgcttcc tgatttatta gggactcacg atttcacctc tttttgtgca tcaggaagct
    45541 cgatagagga taaaatacgt acgatctacg aggctaagat ggaggtaaat gaaacaaaag
    45601 acgaattgtt gtttacattt aggggaaatg gcttcttgta taagatgata cggatcttag
    45661 taggaacgct tttgaaaatt ggcaatggcc gattgcctga agatagtatc ccagatatta
    45721 ttgcgaaaaa agatcgtaat gcagcaggac ctacagcaca ccctgaagga ctttatttgt
    45781 atgaagtgaa atatgaataa gaggttatga tagaagtggt cagcttcaag aaataagaag
    45841 gtatttacga aaattacttc tcaaaatttt gcaaaatttc ggcttatttt cgaagaagct
    45901 acttctggaa caccgtttaa gaaagaggtc gtgagcctgt catttaactc tgagtcacaa
    45961 agagcaattt caaaaaacag cttctcatct ttgatgaata tcgtgacttg tggacgaggc
    46021 gttgataagt gaatcatctt tttgttacct attttttctg taaccaaaaa acgatggaac
    46081 gttgttttga ttgcccttgg tgcggacagt ctgaacaggt tccatcgttt ctttattttc
    46141 tacgtcttct tctgtgtgtg tttcgtggtt gttcttctat tgctggatca atcgtgccat
    46201 aaaaggggcg attgcggtca aggtgccaga cataaagaat aaacaatgca atacatagga
    46261 caaaaaggat tgcacctatc gtaaatcctt caggcaagta actgaaagtg acagtatgtt
    46321 tacctgctgg aacttttatc atcaagaatg cgtctttgaa attgctgacc ttgactggtt
    46381 tcccatcaat tttagctttc catcctttgt cataaggaat agttgtcacc aacatggaat
    46441 cttcagcggt atttacagtc ccagaagctt ttctgccagt ggcttccatt tcgacacctt
    46501 tttctttgat ctgattcatt gcggcttgat aggctacaac atctagacca acgactttag
    46561 gctccatgaa acttatttct ttcgtcccat aaaaacttgc agtaaatgtc actgttgtct
    46621 cttgaggata atagccaaga tcatagtatt gaccagaaat gttgatttgc gtttttcgcg
    46681 atgtaccatt gacagtgatc gtcgcactag aactttctaa ttgcgcaaaa ttggttggga
    46741 acaaacttaa gtaagcttgt gtatttgcag gcacatggac ttcccaagtg agctcttttg
    46801 gctggttatc tgctgtttct gagtaagtga cgccagtcgt tgtctttgtg atcgtcgtat
    46861 tcaactgatt cttcaaagta ggtgtataga atgtaaaata ctgttcattt agaccagaca
    46921 atgtattgat caagtttgtc tgactggtca aattatctgt cactggctgc tcaacatcat
    46981 taacggacaa cggtgcagta aatgctagtg gcaatgcatt gacattctga tacagtgtat
    47041 agtcgccgct ttgaccagct tggcggaagc catatttact gaacgtgctt tcgtttttag
    47101 aaatgttata tttgattcct gtaaacccat ccatcagcaa agtattattg ttataacgga
    47161 tattcaaatt tgtccctctt gaacggaaac ctaatttatc caggtaagag gaagaattac
    47221 gatttcttat agaagaaaac aaactgatac cactgtaacc atagttgatc gaatcgtttg
    47281 cagatactgg atccagattt tccaagcggt aaaacgagtc ggaatctttt tttgtttcat
    47341 ttacaagagc tttcaagtca ggataaggat cagtatataa actgcgggaa gcataattcc
    47401 aatcatcaag aatgccatgg atcatactgt tcgtattgac cagtgcttca gcagacatga
    47461 tcaataataa caatatactc agatgattga atgaaagttt tttatgctga taaaatccca
    47521 cagcaaaagc ataaatccct aagaaaacca ctgtcaatac aaaggaagtc atcgtcacat
    47581 aggtgtagct tccttcaggt ttgatgcccc atgccaccgc aaagagagca gctacgacca
    47641 aaatgattcc taacaacagc ccacgatcgt cttgttctat cttttcccat ccttttcctg
    47701 caagcaggag cactaagaaa gagaacagat aggcataacg gaataaaaac atattcggtg
    47761 catgcatacc atgccagaac aaattcaatg gaacaagata aaaacttgcg ataagtatgg
    47821 cgaaaagact gccgtacaaa aatttatttt tcaacggtac tttccgactg gcaaaataaa
    47881 acagacagag gactaatggt agcagacctg cgtaaacgaa cggaatcgat ccgtattttg
    47941 tcgtgtcata aacaccaatc atatttttca tgattagatc cagataagaa gtcgcttctg
    48001 ttttcaacgt agtgatctga ctcagtgcct cgccatttga ccgaagatcc agcactgctg
    48061 gcaatacaat gatcattgaa gcgccgcctg ccagtaaaga agtgatacca taaggaacaa
    48121 tcgttttttt atattctgac cagtttgtca atagacgggc gataaaatat aagaaagaaa
    48181 atacgccaat cataaaaccc atataaaaac tggataaaaa caagagtaaa taactgataa
    48241 ataaaagttt cggccgttta agatccataa cacgatgaat ccccaaaatc actaatggaa
    48301 gataaacaaa tgcatccagc cacatgatga tctccgaatg tgcagtgata aatgagatca
    48361 atgcgtaact gacgctaagt gcaacatgcc cccagcgagg aatcttgaac gtacgtgcaa
    48421 gaaaccaaaa agccaatccg gcacagccga ccttaagcaa agtcagaaca tacagtgcat
    48481 ctggcatcat ctggtttgga aaaaagaaca ccagtggcgt gaagatcccg cctagatagt
    48541 aagaaattaa agataaataa ttcaatccta gagaagcatt ccatgtataa aaaatacttt
    48601 gctttccaag aagaacattt cggaagctgg catgaaaatt agaaaactgt gaaaaggcat
    48661 cacttgccaa aatacttctg ctgcttccag gatagattcc gatacttaga taaacaagtg
    48721 ctaaaatcaa aaacggaatc aggaaactag ctgctgcgta gcgtccgttt tctctaaaga
    48781 atgactttaa attttttttc atatttattt tccaccaaat ttctatgaag ttgtattcag
    48841 tttatcatat ccttgttttt ctctgcttaa aaagagtcta gttttcacaa aaaaataaaa
    48901 tttagaataa ataacttcat aatttgttcg tataacaaaa ataaacgcaa aaaaagggta
    48961 ggaatcaaaa gtcccaccct taaaaatttt tattaaataa aaaaccgttc actatctgaa
    49021 cggtccaagt atcattcaat taaacgcgtt caacttttcc tgatttcaaa gcacgagttg
    49081 acacccaaac ttttttaggt tttccgtcga ccataacgcg aactttttgt aagttaggtt
    49141 taacagtacg ttttgttgag ttcatagcgt gtgaacggtt gtttccactt tttgtcttgc
    49201 gacctgtaaa gtaacaaact tttgccatgg taatttcctc ctttgccttg atttggagca
    49261 atagaataat tcagctcata ccagactaac atatcatata gaatgggaat ttgcaagtac
    49321 attttcatag atacaattga caattgagaa gaataattgt aaattaaaca aggttatacc
    49381 tgaaattttg caaggttttt gagtactttg ggtaactgcc ttttatttca agttgcgcta
    49441 tgataaaata tgatagaaga aaaatgggag ttttttaccc gttaaggagg ctcattcatg
    49501 gctgtaaaaa ttcaaacgtc agctggaacc attgaaatta ccaatgatgt aattgcaaca
    49561 gttgttggtg gcgccgcgac agatgtgttc ggtgttgtag gaatggccag caaaaatcaa
    49621 attaaagata atattactga gattttgcgt aaagaaaatt actcaagagg agtagttgtc
    49681 cgccaagaag ataatggtat tgctgtagat gtttatacga ttatcagcta tggtacgaaa
    49741 atctctgaag tttcccgcaa cgtccaagaa aaagtgaaat acaatctaga ggcattatta
    49801 ggcgtaactg ccaattcagt taatgtcttt gtacaaggtg ttcgtgtact gccagactag
    49861 gcaggagact ggctggtaac tcagcagtcg atctgaagga ggattatcta ggtgagtgta
    49921 acagaaataa gtgcaagcca gttccaggaa atggtccagg caggtgcgaa tcgtctgcaa
    49981 gtaaatgcag aatacgtcaa ttcattaaat gtattccctg tgccagatgg cgatacggga
    50041 acaaatatga atctatctat gactagtgga gctaaggcag tagtagattc aacctcagaa
    50101 aaagtaggag aattagcagc aatgctttca aaaggattgc tgatgggtgc gcgcggaaat
    50161 tccggcgtta ttttgtctca attgtttcgt ggattttcta agcaaattcc agatgttgtc
    50221 tcattaaatg cttctgatct tgctgcagcc ttcactcatg gagttgaaac tgcgtataaa
    50281 gcagtaatga aaccagtaga aggaacgatt ttgacagtag ctagagaagc agcgaaagct
    50341 ggagaaaaga aagcaaaaag tactgatgat gtcgttgaag taatgacagc tgttgtaaaa
    50401 ggagcaaagc gtgctttagc taaaactcca gacttgcttc cagtcctaaa agaagtcggt
    50461 gtcgtagaca gtggagggca aggactgctg tttgtttatg agggcttttt aagtgcattg
    50521 aatggagaat tccaagcgga tgaaacatat gaaccttctc cagcagaaat ggatgatatg
    50581 gtcaatgcag aacatcatcg cagtgtccaa ggtcaaatgg cgacagaaga tatccatttt
    50641 ggttactgta cagaaatcat ggtaaaaatc ggagaaggac ctacagtcga tagtacattc
    50701 gattacgaag aattccgtaa ctatttagat ggtataggag attctttact tgtggtcaat
    50761 gatgacgaga tcatcaaagt ccacgtgcat acagaaaacc caggagaagt cttgaattat
    50821 ggacaaaaat tcggttcatt agtcaaaatc aaagtcgaca acatgcgctt gcagcatgag
    50881 acgattttgg aacacgatga agaagtgaac gagtttgcga aaaaagaaat accacgtatc
    50941 ccgtatggta tcgtagcgat cgctgctggt aaaggcgtcc aagagctgtt tgaaagttta
    51001 ggcgctaatt atgttatcag tggtggacaa acaatgaatc caagtacgga agacattgtt
    51061 aaagcgattc aagaagtaaa cgcagataaa gtgattgtct tgccaaacaa taagaacatc
    51121 ttcatggcag ctgatcaagc agcagaagta gcagatatcc cagtagcagt tgttccttca
    51181 agaacgattt cccaaggatt gacagctatg ctttcattca atgatcaagc ttctttagaa
    51241 gaaaacaaag aaacgatgac agaaatgttg tctagcgttg tgagcggtca agtcactcac
    51301 gcgatcagag atacctcaat tgacggtgta gagatcaaag aaggagactt tttgggcatg
    51361 atcgatggaa aaatcctgat ctctaaccca gatatcacag caacagcgtt aagtacacta
    51421 gaacaaatga tcaatgagga cacagagatc gtaacgatct tggcaggtga tgaaggaaca
    51481 gttgaacaag caaatgaatt tgctgagaaa ctaacagaaa aacatcctga tttggaaatc
    51541 gagatccacc aaggagatca gcctgtctat ccttatctat tttctgctga ataatttctg
    51601 tgtcagaata tctttctaac acagtattca ttcataaaag gccttcggtt agtgatcagg
    51661 tatgatcgct gaccaagtcc tttttttgtt aaaggaggga ggaaaaattt atgcgttctt
    51721 tagatgatcc aatcactatg ctggcaggag tcggagaaaa aagagcagct agtcttgcca
    51781 gcttgggcat ccaaacgatt gaagacttac tgacttatta tccttttcgc tacgaagata
    51841 ttcaggaaag aaatctaaac gagatccagg atcaagaaaa ggttactttg aaaggaatcg
    51901 tagtctcgcc tcctgtgatg aatcgatttg gttataaaaa aagccgattg caatttcgta
    51961 tgatgcaaga acatgatgtg ttcaacgttt ctttttttaa tcaaccttat ctaaaagaaa
    52021 aagtcatttt atcggaggaa atcgccgtat acggtaaatg ggatgcaaag cgtaaagcct
    52081 tgaacgggat gaagatttta ggttctcaaa caatggatga cttttcgccg atatatcacg
    52141 tcaacaaatc aattcgtcaa acgacattag ttgatctaat caaaaaaggg tttcaagaat
    52201 acggcacatt gattgaagaa aatctgccaa acaaccttgt ggagaaatat cgactgcttg
    52261 accgagcttc tgctgtaaga agtatgcatt ttccaaaaga ccacgaagaa aaccatcaag
    52321 ccaaacgacg tgtcgttttt gaagagttct ttttatttca aatgcgtctt caaggattga
    52381 aaaaagcaga aaaagcagaa acgaatggtt tggagatcct ttatgatgtc caaaaattga
    52441 aacaatttac tcaaaagcta ccttttgaac tgacaaatgc acaaaaacgt gtaacgaatg
    52501 aaatctgtcg ggatcttcgc agtccgcagc atatgcagcg acttttgcag ggagatgtag
    52561 gtagcggaaa aacagtggtt gccgcgattg cgttatacgc taccgttaca gcaggtttcc
    52621 aaggtgcgtt gatggtgcct acagaaatct tggcacaaca acatatggaa agcttgcaac
    52681 agctgtttga tcccaatgaa gtaaccatcg cattgattac ggggtcaacg aaagcaaaag
    52741 aacgacgtga gctccttgaa aaactagagc aaggggaaat taacgtcgtg atcggcacac
    52801 atgcattgat ccaagatgga gtcgaatttc agaatttagg tctagttatt acggatgagc
    52861 agcaccgttt tggcgtgaac cagcggaaag tacttcgtga aaaaggctgg cgaccagatg
    52921 tcttatttat gacagcgact ccgattccta gaacattagc tattacagca tatggtgaga
    52981 tggatgtatc tatcattgat gaacttccag ctggacgtat cccgatcgaa acacgctggg
    53041 taagaacacc tcaattggac agtgtactgg attggacgaa aaaagaactg gcaagaggac
    53101 atcaaatgta tgtcatctgt ccgctgattg aagaatcaga agcattagat gtaaaaaatg
    53161 cagtggaaat ctatgaaaaa cttcgtgaac tatttgcacc aaaatatgaa gttggcttgt
    53221 tacatggtaa aatgaagaat caggaaaaag aagtaatcat ggaagccttc aaagacaacc
    53281 agatgcagat attagtttcg actactgtaa ttgaagtagg ggtaaacgtg ccgaacgcga
    53341 ccgtcatgct gatcatggat gcggatcgat ttggattggc acagctgcac caacttcgtg
    53401 gacgtgttgg acgcggaagt gatgcttcct actgtatctt agttgctaat cctaaaaatg
    53461 aactaggtgt ggaacggatg aaaatcatga cagagaccac caatggtttt gtactaagcg
    53521 aaaaagatct ggaattacgt ggtcctggtg aagtatttgg cttccgtcaa tctggtctgc
    53581 ctcaatttgc gattgctgac ttggtgacgg atggaaatgt tttggaggta gccagagaag
    53641 aggctgcagc cctttggcaa ataaaggatt ggcagatgct tcctgagtat cagcctttag
    53701 cgcaaagcct ccaaatagaa aatcaggata atcgttattt cgattagcag actgtgctat
    53761 actttatact gatgacggaa aaaggagtgg aaacaatgaa aattgccgta gatgcaatgg
    53821 gcggagatca cgcacctcaa gcaattgtag aaggcgtggc tttagcgcag caagattttc
    53881 cagaagtaga attcttactt tacggaaaag aagcagaaat caagaaatac ttaacaaatg
    53941 aaaaaaacat cacgattatc catacagacg aaaaaatcaa tagtgatgat gagccagtaa
    54001 aagcaattcg acgaaaaaag actgcatcaa tggtccttgc tgcacaagca gtgaaaaacg
    54061 gtgaagcaga tgctgtattt tcagcaggga atacaggcgc gctactagca gcagggttgt
    54121 tcatcgtagg acgtatcaaa aatatcgaac gccctggctt gatgtctacc ttgccagtag
    54181 tagggaaaga aggtgccggt ttcgatatgc tggatcttgg cgctaacgca gaaaacaaac
    54241 cagaacattt gttgcaatac ggtattttag gttcttttta tgcacgtaaa gtccgaggta
    54301 tcggacgtcc tcgtgtcgca ctcttaaaca acggaacaga agcaacaaaa ggtagtgaag
    54361 tgacgaaaaa agcctatgaa ctccttcaga atgaaacaag cttgaatttt attggaaacg
    54421 tagaagctcg tgagctactc aatggtgtgg ctgatgtagt ggtcacagat ggatttacag
    54481 gcaacgctgt tttgaaatct attgaaggaa cagcaatgaa tatgatgaat ctattaaaat
    54541 ctgctattct aaatgaaggg atcaaaggaa aaatgggcgc tatgctttta aaagacggct
    54601 tacgttcttt gaaagcagaa atggattatt ccaaacatgg cggtgccgtt ttgtttggct
    54661 tgaaagcacc agtcattaaa actcatggag caacaggccc cgatgctgtt cgctatacga
    54721 tccgccaaat ccacacaatg cttgagacag atgtagttgg acaattagtc gaacaatttg
    54781 aaaaaagcga agaatagtat ttggatatct gaagcgatcc gttccattga aaaaaaggtg
    54841 gacattcctg aaaaatgcca gtaaaattag ccatggtgta ggtaattgac aagtgtatga
    54901 ttcttggagg tgaaaaagtt gactcgcgaa gaagtattta acaaagtagc gaaaattatt
    54961 tcaaatcatt ttgagataga taccgataaa gtgacggatg aattgaacat caaagatgat
    55021 ttaaaagctg attcaattag tattatggaa ttcgttttag agcttgaaga tgaattcggt
    55081 acggaaattt cagatgaaga cgctgaacaa attgaaacag tcggtggcgc agtagattat
    55141 atcagcagcc atcttaaata atttcaaata aaagcaaaga aggataaaaa gctgcatgca
    55201 gctttttatc cttctttttt taatcttttt gacacgtaaa agtcattttt ctgataattc
    55261 tggcgataaa cagaaattat gttgcaaagt aaagcggttt tctatataat tattaataat
    55321 tcattacaca aagataagaa taacctttgt tataaaacga tttattctgt gtaatgtcct
    55381 tttttattaa gaaaaacttc catttaggaa gcagagaggg gtattcgatt gtccgataac
    55441 caattattag aagttcagaa cctacataca ggattccgac tcaaagatga ctattacgat
    55501 gcagtagatg acgtttcatt tactttagac aaaaacgaga ttttagcaat cgttggggaa
    55561 tctgggtgcg ggaaaagtac cttggctact acgattatgg gattacttga tcctaataac
    55621 acaaaaatta caggtgagat tttatataac gatctgaatt taatcgacct gaacgaaacg
    55681 ctctataata aaatccgcgg aaatgatatc ggaatgattt tccaagatcc gttgtctgca
    55741 ttaaatcctc tcatgagaat cgaagaccag atcaaagaag gactttccta tcacacaaag
    55801 atgaatgcag aacagcgtca agctcgtgca ctagaattgc tagaacaagt agggattcct
    55861 aatcctgcga gagttggacg ccaatatccc catgaactat caggcggtat gagacaacgt
    55921 gtcatcatcg caatcgcaat cgcctgcaaa cctccaatcg tcattgcaga tgaaccgacg
    55981 acagccctag atgtaaccat ccaagcacag attcttgatt tactgaaaga ccttcaggaa
    56041 gaaacaaaaa cagggatcat cttgatcact catgacttag gagtcgtagc agaaatggct
    56101 gatcgagtgg cagtgatgta tggcggacag ttcgttgaag tagcaggtgt aaaagaactg
    56161 tttgatcatc ctaagcatcc ttatacacgc tctttactga actctatacc acaagaagga
    56221 aatcatgatg cagaactcca tgtgatcgaa ggtgtcgttc cttctctgaa aaacatgcct
    56281 cgtaaaggtt gtcggtttgc accacgtatt ccatggatac cagcaagcga tcatgaagaa
    56341 gagcctatcc ttcatgaagt cgcaccgaac cacttggtac gttgctcctg ctataagcat
    56401 ttccacttta gagacgaaaa aggagaggtg tagatgacag aattaatcac aatcaaagac
    56461 ctgaaagttc attaccctat tcgcagtgga ttcttcaatc gagtaacgga tcatgtatat
    56521 gctgtagatg gtgtggattt tattatcgaa aaaggaaaga catatggttt agtgggagag
    56581 tctggctctg gaaaatctac tacgggaaaa gccgttgtgg gattagaaaa ggtaacagcc
    56641 ggacaaatca tgtatgaagg aaaagatgta acgaagcgaa gcaaccgcaa aaaaatgggt
    56701 tataacaagg atgtgcagat gatcttccaa gattcaatgt ccagtttgaa tcctaaaaag
    56761 cgagtgttgg atatcattgc tgaacccatc cgaaattttg aacggttaag cgatcaagaa
    56821 gaaaagaaaa aggtaaaagg actgctagat atcgttggga tgccggaaga tgcgctatat
    56881 aaatatcctc atgaattttc aggcggacaa cgtcaacgtc taggagtagc ccgtgctgtt
    56941 gctaccaatc caaaactgat cgtagccgat gaacctgtat ctgctctgga tctatctgtt
    57001 caagcgcaag tattgaattt catgaaacga atccagcaag aatacggcct cagctattta
    57061 ttcatttcac atgatttagg tgtagtaaaa cacatgtgcg acaatatcgc aattatgtac
    57121 aaaggtcgat ttgtagaaat cggtacaaga gaagacattt ataatgaccc gcgacatatc
    57181 tatacgaaac gtctgctttc agctattccg caaatcgatg tagaaaatag ggaaagccac
    57241 aaagaaaatc gacgaagagt agaacaagag tatatccaaa accaaaaaga atactatgat
    57301 gcgactggtc gagtctacga tttgcgtacg atcacaacta cacacaaagt ggcgttgaaa
    57361 gatggaggtg caagctaatg tggaaaacga ttctgcgacg tgttttattg atgatcccgc
    57421 aagttattat tttaagtgtt ctgatcttct tattagccca agcgatgccg ggggatccat
    57481 ttaccggttt gatcaatcca aatcaggatc cggcagtgat cgaacagatg cgacaagcag
    57541 ctggcttgaa tgacccatgg tatgtccagt acttccgttg gatcggaaat gctttgcaag
    57601 gcgattttgg tcaaagtttc ttatacaaac gctctgttgc tgacttgatc ggcgaacgga
    57661 tcatcaacac tttatatcta tcgatcttga cagttgtcct tacttatttg attgctgtgc
    57721 ctctggggat gcttgctggc cggtatcaaa attcaatctt ggataagatt gtcgtaatct
    57781 acaacttctt tagttttgct gttcctttat ttatcttcgg cttgatcatg ctatttgttt
    57841 ttggttatcg tttaggctgg ttcccaacaa gtggatcaca aacactaggt ttgcaaggtg
    57901 gatttatcac tcaatggata gaccgtttga aatatattct gttaccatca attacccaag
    57961 catttttggc gactgccgtc acaatccaat atctgcgaag tgaagtaatc gattcgaaat
    58021 cgctcgattt tgtcagaaca gcccgttcca aaggggtacc gacgaataaa gtattcagtc
    58081 gacatatttt ccgaaatgca gcgttgccga tggcttctca aatggggtat gaaatcactt
    58141 cgttgattgc tggttctgta gttatcgaaa agatttttgg ttatccggga gtcggaaaac
    58201 tatttattga ttccatcggc caacgggact ataccgtgat cacatcctta gtattgattt
    58261 taggaatcgc gacgctagtg gggacattac tttcagatat catcatgagt atcgttgacc
    58321 cgcgtattcg gattcaataa aaccaatcag aaaggagaat ggatcgatga gtgaaaaaaa
    58381 caatacagca gttcaagaaa gtattcctcc tatggggata cgaatgattg ctcgagaatt
    58441 taaaaaagat aaaattgcta ttttttcatt aggtttgcta gtggttttat tattagtgat
    58501 ctttatcggt gcgatgctga ccgatcaaga caaagtgatg tatgttagta tcttcgacaa
    58561 atacgctgca ccaggaacag tttcgacaca aggacaaaag ttcattctgg gagcagatga
    58621 aggcggacgt gatgtattag gacaattgat cataggtgct agaaactctg ttacgatcgg
    58681 atttgcgatc acgatcctta cttcgatcat cggggttgga ttagggatca tctctggcta
    58741 ctacggaggg atcattgaca acatcttgat gcggatcgtt gattttatca tgattctacc
    58801 gatcatgctg atcattatcg tcttcgtatc gattatttcc agatacaatg tctggtcctt
    58861 tatattcatc atgagtgcat tttattgggt agctaaagcc cgattattcc gaagcaagac
    58921 attatctgaa gcaagaagag attatgtcag tgcctcaaaa acaatgggaa ccaatgattt
    58981 taaaatcatg ttccgcgaaa tcatgccgaa tctaagttca ttaatcatta ctaatttgac
    59041 gatgaacttt gcggcaaaca tcggtatcga gacaactttg actttcttag gtttcggctt
    59101 gccggcaaat gtacctagtt taggtacatt gatcggttat gcaagtaacg gagaagtctt
    59161 agtgaatcgg caatggatct ggttacccgc atcaatcctt attttagttt tgatgttgag
    59221 tatcaattat gttggacaag cattcaagcg ttctgcagat gcgcgccaac ggttaggcta
    59281 gtatagaagc gaagggggaa ataggaatga agaaaacgat ttttggattt gcagcgctga
    59341 tgtctgctgt cgttcttgca gcatgcgggg gcggaggtac agatacctct ggaaacaact
    59401 ctggaggcaa cggcaataac aaagaggaat taacttttcc aattgcaaca aaaaataatg
    59461 atgatgcaat cgaagacggc gaattagaag ctgcggtcgc aacggacagt cagtttaaag
    59521 gattgttcca gtgggagttt taccaagatt catatgatgc agcttttatg gctccttctc
    59581 atgaaccgct atttgcaaac gatgacgatt tcagaatcac agacgacgga gctgcaacgc
    59641 ttgatctaga tgaagatacg aaaaaagcga caatcaaagt aaaagacaac gtcaaatggt
    59701 cagatggtga agatgtaacg gcggacgatg tgattttccc ttacgaaatc attgggaata
    59761 aagattacac tggtatccgt tatgacgaca ctttccgtaa catcgtaggt atggaagaat
    59821 atcatgatgg aagtgccgac acaatctccg gaatcaaaaa aatagacgac aaaacagttg
    59881 aaatcgaata taaagaagta aacccaagca tgcttcaatc gggtggtggt atctggtcat
    59941 atgcagcacc aaaacatacg ttagaaggtg tagcaatcaa agatatggaa tcaagcgatc
    60001 aagtacgtaa aaatccagtg acttttggcc cttattacat gagtaatatc gttgcaggag
    60061 aatcagtaga atacttgcca aacgagcact actggaatgg tacgcctaaa ttgaaaaaga
    60121 tcacgatgaa atctgtaccg atggcaagtg caactgaagc attgaactct aaattatatg
    60181 atatgatttt ccaaatgcca acagatactt acgataccta taaagaaatt gctggctata
    60241 caaacttagg ccgtgaacaa acttcttata cttatctagg attcaagtta ggtaaatgga
    60301 atgcagatac aaagagtgtg gaatacaatc cagattcaaa aatggctgat aaatcattac
    60361 gtcaagcaat gggctatgca ttagataata atgcagtagg tgaacgtttc tatgcaggtt
    60421 tgagaagcaa tgcgacttca ttgattcctc cagtattcca gtcataccat gatgatgaga
    60481 tgaaaggatt cacacaagat ctagacaaag cgaaaaaact attagatgat gctggctaca
    60541 aagatgtgga tgacgatggt ttaagagaag acaaagatgg aaataaacta acaatcaact
    60601 ttgcttcgat gtccggtggg gaaacagcgc aaccattggc agattactat atccaacaat
    60661 ggaaagaaat tggtttggat gtccaataca caacaggtcg tctgatcgaa ttcaattctt
    60721 tctatgataa attagaaaac gatgatcctg aaattgatgt ttaccaagct gcttggaata
    60781 caggaactga tccaaatcca agtggactat ggggaccaaa tgctgcgttc aactacactc
    60841 gttatgaatc agaagaaaat acaaaattga tcaacgatat cagttcaagc aaagcatttg
    60901 atgaagagta ccaaaaagaa gcatatcaaa aatggcaaca atatgcattt gaagaagcat
    60961 ttgcgattcc aacgttgttc cgtaatgaag tattgccagt caacgaccgt gtcaaagact
    61021 gggattggtc ttatgatgta ccaaacccat gggcaaatgt ttctgttaca gcagattcac
    61081 gttcataatc actaaaaatg accgaaaaag ggcgatgtcc tttttcggtt ttttgatatt
    61141 tttctgttta tttctctaat attcaggtca aggcgctgat ttcatgactt ctccaataaa
    61201 cggtgttcaa agtccagttt ctcggtatta agtcaaattt atgcaaaaat atggaaagcc
    61261 attttcacaa aattttttct tcatactcga aactaaacga atttttcaca acttctcgtt
    61321 aatcactagg attattaaag ctttacttac cttttgaccg aaccatttca aatttgacaa
    61381 aaaaccagta taataaggaa gtatcgaaaa aatatccata aaaggaatgt ggaaaatgga
    61441 caaccagctg acaaaagaac taaaagaaaa ctataacatc gtttttcacg atctaaattt
    61501 gcttgaacaa gcttttaccc attcatccta tgtgaatgag catcgcaatc tgcaattatc
    61561 ggataatgag cgacttgaat ttttaggaga tgccgtttta gaactgatgg tttctcagta
    61621 tttattccgt ttatatcctc atttgccaga aggaaaattg acaaaaacac gagctgcgat
    61681 cgttcgcgaa gacagcttgt cgaaatttgc caaagaatgt cactttgatc aatatgtttt
    61741 gttgggcaag ggagaagaga attcaggagg acgtacgcga ccagcattgc tttgcgacct
    61801 attcgaagct tttttaggcg cgttatatct tgaccaggga ttcgacacga cccatgcatt
    61861 catcgaaaaa gtgatttttc ctaaagtcaa agcaggtgct ttttcacatg agatggatca
    61921 taaaacaaaa ctacaagaag tcttgcaaaa atcaggtgat gtatcaatcg aataccgatt
    61981 gatcaatgaa gaaggtccag ctcatgaacg agtattttgg atcgaagttt atgtagatga
    62041 tcaattgatt ggcactggac aagggaaatc gaaaaaatta gcagaacaag ctgcggcgga
    62101 aaatgcattg gcagcactgt aaaagtaagt gagggaaagc tttgtatttg aaacgaattg
    62161 aaatagccgg ttttaaatca tttgcagaca agacagtcat tgattttgaa aatagcgtca
    62221 cagcagtggt tggaccaaat ggcagcggaa aaagcaatat tacagaggct atccgctggg
    62281 tattaggaga acaatctgct aaaagtctgc gaggcggaaa aatgccggat atcatctttg
    62341 ccggttccga tacccgtaag cagctgaata ttgcagaagt cacagtgatt ttagacaata
    62401 gtgatcatta tctgccattg gagtacaatg agatcagtgt aacccgccgt tatcgacgga
    62461 ctggggaaag cgagtttttc atcaataaac aaagctgcag attaaaagat atccaagaat
    62521 tgttcttaga ttctggatta ggaaaagaat cgttctcaat catttctcaa ggaaaagtag
    62581 aagcgatttt cagtagtaaa ccggaagacc gtcgtgggat ctttgaagaa gcagctggtg
    62641 tcttgaaata caagcagcgg aaaaagaaag cagaacagaa gctttttgaa acagaagaca
    62701 acctcagccg cgtccaagac atcatccacg aattggaaga acagttaact cctttggccg
    62761 cccagagtga ggcagcaaag gagtttttac ggctaaaaga aacactgact caaaccgatg
    62821 tctctttgat ggttgctgaa atcaagacag caaaaaaaga ttgggacaac aaacaagcac
    62881 agcttgcgaa attcaatcta gaattaggca aactatctga gtcgatccaa gaacaagaat
    62941 ccattttggc gaagcagcgt aaagaaaatg cgcaagccga tcgtctgatc gaaaaaaatc
    63001 agcaagtatt attagatctt tcggaaaaac tgaagcagac agaaggacaa aaagatgttt
    63061 tgcaggagcg gacaaagcat acacaaaaaa gctcgcaaga atatcaaact tcattagcag
    63121 aagcacaaaa aaaggttaaa cattttgaaa agcttcaaga aagcttgatg aaagcagcag
    63181 cagaaaaaga aacagagatc caaaaagctg aagcaaatct cataaaaaca cagcaagaat
    63241 tagaaaaata ccaaaaatca acgaaagaat tattggcaga attaagagat caatatgttg
    63301 atttgatgca agaacaagca gctgtaggaa acgaactgaa gtatctagaa cgtcaatata
    63361 tacaagaaac agcaaaaagc aagcagacgt tagccaagca atcggaagta gaagcatcag
    63421 tagaccgatt gatcttacaa aaagaagaat tgacacaaaa gcaggcgcag ctaaaatcat
    63481 ctttaactga aacaaaagag aagttagaaa tgatccaaca aaacgggaaa aagttccaag
    63541 aagcattagc aaaagaacag cctaaaatgt atcaattaat gaatcaagtc cagcagctac
    63601 gtgctcgtaa gaaaagcctt caagaaatcc aagaaaacta ttttggtttt taccaagggg
    63661 ttcgattggt cttgcagcat aaacagcagc tttcgggtat tgtcggagca gtggctgaac
    63721 tgatcgatgt tcctgcagat ttcacgttag ctatcgaaac agctttaggt ggggcagccc
    63781 agcatgtcat cgttgaaaat gaaaaagatg ctcggcaagc catcacgtat ttgaaacagc
    63841 aaagaggagg aagagcaaca ttccttccac tgacgacaat caaaccaaga caattaccgg
    63901 ctcatatatt gacacaagca gcagctgtag aagggtttat agggattgcc agtgaacaag
    63961 tcagttatcc cgatcaaatc caaactgtgg ttcaaaacct gctaggaacg atccttttag
    64021 ccaaagactt aactagtgcc aatgcaatcg cgcaaacgat ccgctatcaa tatcgagtgg
    64081 tttccttaga aggtgatgtg atgaatgccg gaggatccat gacaggtgga gcgaataaac
    64141 gaggaaacca aggcagtttg tttgtacaaa atcaagaatt gaaacagttg actagtgaat
    64201 ttgaagaagc agataaacaa ctccaagcac aagaaaaaaa agttcaggag ctgcaacaag
    64261 aaacagctcg tttagccgaa gaacaagaag ttttgcgtac acgaggcgaa caattacgtt
    64321 ttgaagaaca agaagcgaca aatcagctac aaaacatcat aaatgaactt gaacgttttg
    64381 aaaaagaaaa gcaaatctca acttttgaga cacgtgaact gcagcagttt atcgaagatt
    64441 acgaaaagca aacgaacgaa ttgaaagaca aacaaacaga tcttgaatcg caacgacaaa
    64501 aaatcgacga agaaatcaaa tcgttaagtc aagaaagcga ccagatggaa gccagaagag
    64561 cacaagtaca aagtcaaaaa gcacaagaac aagcagatct tgctgtcttg aaagagcagt
    64621 ttaatcatct gcagatccag ctccgcggtg cccgagtcca aaaagcagag gcaaccgaac
    64681 gtcaagaagc gatagaaaag cagttggcga cattgacagc agacttttct gatcatgaag
    64741 tcacagaaga aagtttggaa aaacaaatta acgaattatc tgctcagcga gaaacattaa
    64801 aagccgagtt ggcaaaagcc aaagagcagc gagatagaag gcaaaaggag atcgataagc
    64861 ttgaagcagt cttggcagag cgtaatcgtg agcaaaaagc acgactgtcg gaacaatcca
    64921 aattagaagt ccaaaaagac cgagctgaaa tgattttaga taatcatttg acctatctgc
    64981 agtcagaata tcagctgaca tttgagaaag cttcccaaga ctatcaagaa acaaccgata
    65041 tcgaagacag tagaacaaag gtttcatcct taaaagaaca gattgaaaaa ctgggaccag
    65101 taaacttaaa ttcaatcgaa caatacgaac aagtcagtga aagacataca ttcttagcaa
    65161 cacagcgaga tgatctgtta gcagcgaaaa accaattgtt tgaaacgatg gatgagatgg
    65221 atgatgaagt gcggacaaga ttcaaagaag tatttgaagc tatccgtcaa gaattcaaaa
    65281 ttgtctttcc aaatatgttt ggcggcggta aagctgaatt agtactgacg gaaccaactg
    65341 atttgctgaa aacaggtatt gagattgaag tacaacctcc tggcaaaaaa ctgcaaagtc
    65401 taagtttgct ttctggagga gaacgagcac ttacagccat cgcgttgctc ttttccatta
    65461 tccgtgtctg tcctgttccg ttttgtatct tagatgaggt agaagcagcg ttagatgaag
    65521 ccaacgtgaa acgttttggt cgttacttga gcgatttcca agatgacaca caatttatcg
    65581 tagtcacaca ccgcaaagga acgatggtcg cagcagatgt actgtacgga gtgacgatgc
    65641 aggaatccgg tgtatcaaaa atcgtttctg tacggatgga agatatcaat gaagaggcga
    65701 aaatcaacga aacgaaagtg taggaacgaa aatgatcaaa ttagtagcaa tcgatttgga
    65761 cggtacatta ttaaatgaac aaaagctgat ttcagatgag aataaacaag cattagcaca
    65821 agcaaaacag caaggggtca agatcgttct atgtacagga cgccccttag ctgcgatggc
    65881 gcattatctg caagaattag gactggtaga tgaaggtgac tacagcatca ctttcaacgg
    65941 cggtttggta caaaaaaatg atactggtga aatcatggaa aagaaagtga tggaagtatc
    66001 agatatccaa cgcttataca aactggctca gcaattggat ctgccactag atgttttgtc
    66061 tgacaatgtg gttttacagc tgcctagtgc cccaaggaag caatccttgt acaatgtgtt
    66121 gaataagctg ctgcaattcc aaccagcgga tataagcgat attacagatg aatttgtgct
    66181 taataaggca gtaatcgctt atgaccaaga agaattagat cctaaaatca aagaaatacc
    66241 agcagaattc catgaaatgt atgagatcat caaaacaaga agtatgcttt tggaatttat
    66301 gccaaaaggt gttacaaaag cttatggtat ttctttattg gcaaaagatc ttggtttgaa
    66361 acaagaagaa atcatggcga tcggtgatga ggaaaatgat ttaccgatga tccaatatgc
    66421 tggactaggc gtagcgatgg agaatgctgt gcctttcgtc aaagaagcag cagatttcgt
    66481 gacagccagc aatgaagaaa atggcgtagc caaagtgatc caaaaatttg tattagagta
    66541 gggggagtca aatgggattt tttgataaaa tcaagcgtgc ttttactggc gaagagaaaa
    66601 cagaagaagt tgaagtacag gagaagtacg acaagggttt agaaaagacg cgtaaaacat
    66661 ttggtgaacg gatgaatgag ttgtttgcca attttcgaac agtcgatgaa gacttttttg
    66721 aagaattaga agaaacactt atcggtgcag atgtaggttt tgaaacagcg atacgaatta
    66781 ccgaagcatt acgtcaagaa gtgaaactca gaaatgcgaa aaaatcggca gaagtccaaa
    66841 atgcgatcat cgagaaactt gtggatctct atgaagcgga aggaatcaat gaagtaaatg
    66901 aactaaatat ccaagaagac ggactaacag ttattttgtt tgtcggtgtt aatggtgtag
    66961 ggaaaacaac aagtattggt aaacttgccc atcagttcaa aaacgaaggt aaaaaagtat
    67021 tgatggcagc agcagatacc ttccgcgctg gagcaatcga ccagttagtt gtctggggcg
    67081 aacgcgctgg tgttgaagtt gttcgtggca atgcaggagg cgatccagca gctgtcgtat
    67141 tcgatgcttt ggaaagagca aagacagagc aggcagatgt tttactagtt gatactgcag
    67201 gccgactgca aaataaagtg aacttgatga acgaattaga aaaaataaaa cgagtaatcc
    67261 agcgggaatt accagaagca ccccaagaaa cacttcttgt attagatgca accactggtc
    67321 aaaacgcgat ggttcaagca aaacaattca aagaaacaac agatgtgacc ggtcttgttt
    67381 tgacaaaatt agatggtaca gcaaagggcg gtatcgtact tgctatccgt aatgaattgc
    67441 atttaccagt gaaattggtc ggtcttggtg aaggaatcga tgacttggag ccgtttgatc
    67501 caaatgattt tgtcgttgga ttgttcaaag gattactaaa agaagaataa cccatgtcta
    67561 aaaagcagga gctgtgacaa aagttgcagc tccttgtttg aatgattgtt tttaacatag
    67621 ctgtgttttt gatgccttca tttttatact tatttgtaag cggttctcaa acaatttcgt
    67681 tctttttctt agactttttg attataatta gattcatatg aattggaggt cacttaatga
    67741 tgaattttaa gcaattatta ttgcatgtca atgcgcgccc ttttatcgac caagctcgat
    67801 ttggtataga acgggaagga cagcgggttg atcttgcagg aaatctagca aaaaccgatc
    67861 atccagcaat ctttggcgat cgatcctatc acccctatat ccaaacagat tttagcgaaa
    67921 cacaaacaga gatgatcacc cctgttaccg attctattcc cgaattattt cagtatctcg
    67981 ctgctgttta tgatgtgact gctcgttcta taccgaaaga agagatgatc tggccattaa
    68041 gtatgccacc tgccttaccg gaaaaagacg aagagatcat tattgcaaaa ttaaaaaatt
    68101 tcgaagatgt cttgtatcga cgttatttag caaaagaata cgggaagcga aaacagatgg
    68161 taagcggtat tcatttcaat tttgaatttg gcgatgagtt gctaagaaca ttgttcagcc
    68221 atcaggaaga atttcaagat ttttccgaat tcaaaacaga actttatttg aaaacagcga
    68281 gaaactttat gcgctatcgc tggatgatca cttatttatt cggtgcttca ccaatgagtg
    68341 agaagaacta ctttttagat gaatcacatc cgcaagaacc tgttcgcagt attcgcaaca
    68401 gtgcgctggg ctatacgaat catccaaatg tgaaagtttc ctatgcttct atgaaacagt
    68461 atttagcaga tatcgagcga atgatcgaag aagggaaact ttcggaggag aaagaatttt
    68521 atacaccgct tcgtttccga ggcggaaaga aagtcgcaga tttagcaaca acaggtgttc
    68581 gttatattga attgcggaat atcgatttga atccttatgc aagattgggt atcaatccag
    68641 agcaagttcg gttcttacaa ttattcctga tgtacatgtt atggacagaa gaaaaagaag
    68701 actgtgacca atgggtagca gaaggtacga ctcgaaataa caaagtagca ctagaacaac
    68761 catcagatca aacagaattt catcaagaag gtagagaaat tcttgaagga atgaagcaaa
    68821 tgctggttga attggattgg ttggattctc tttatttagt agaggaagca ttgactcaaa
    68881 tggatcatcc tgaacaaacg ttagcagcga aactctatca agaagcacag ctatcgagtc
    68941 agcaagaagt tgctgtggca ttgggacatc aatattataa agaaagccat gaacgtccct
    69001 atcagttggc cggttttcgc gaaatggaac tttctactca gatatttatg ttcgatgcca
    69061 tccaaaaagg tgtccaagtc aaagtattag atgagtcgga tcaatttttg cgtctgcaat
    69121 tccaagacca tgtagaatat gtgaaaaatg caaacatgac aagtaaggac agctatatcg
    69181 tcccacttat catggaaaac aaaacagtaa cgaaaaaagt cttaaaagaa gctgggttcc
    69241 gggtaccagg aggcgcagaa ttttcatcca tggaagaagc ggtaaaagct tatccgagat
    69301 ttgccgagca ggcatttgtg atcaaaccta aatcaaccaa ttacgggtta ggtatcacga
    69361 ttttcaagga aggagctagt ctggaagatt atcaagcagg tttagccatt gcttttcgtg
    69421 aagacagttc ggttttagta gaagaattta tgccgggaac ggaatatcgt ttctttgtga
    69481 tcgacggaga agtccaagcc atcatgttgc gagtccctgc taatgttatt ggtgacagta
    69541 tccgaacagt gaaagaactt gttgaagaaa aaaatagtga ccctttgcgt ggaacgaatc
    69601 atcgtgcacc tttagaattg atccaattag gtgaattaga acaattgatg ttgaaagaac
    69661 aagggttgac gatcgaatca gttccccaag ccaatcaaat tgtttatcta agggagaact
    69721 caaatatcag tacaggtggt gattcgatcg atatgactga tgaattttct gaagcttaca
    69781 aaaaaatcgc cgtttctgcg gtagaagcac taggagctaa gattagtggt atcgatttga
    69841 ttataccaga taaagaaatc gatcctacaa ctgataaaaa agcatatggg atcatcgaag
    69901 caaatttcaa tccagccatg catatgcatg tttatccttt tgcaggaaaa ggcagaagat
    69961 tgacgatgaa tgtattgaaa ttgttatatc ctgaagtatt ttaaaagaaa aagcttttga
    70021 cagattgtcc tatttgatgt caatctgatc aaaagctttt tttgcatgta aaaatttatg
    70081 tgttttttcg ttttaaaata aacgatccca gaagcttttt ttcttttttg gtttttcttt
    70141 ttcagctggc gaagagtttt cttctgggta ttttttttcg atatcaatca caggttcatt
    70201 tactgcttct tttgctgtta agagcaatcc gaattcctca ggatttgcag tgacgtaatc
    70261 gttgactaca gtgaaacggc tattgctttt cgttgctagc tggatatatt ggttttggac
    70321 agaaaggggt agagccccat tcaagagtat ggaagcatct ggatgttttt tgagttcttt
    70381 taaaaagttt tctttgtttt ttggttggat catttcagca acagtcatac taagatagca
    70441 gcgttccctg aatgtcccta aatatttatg ctgttcttca gggttcacta aaggtgtacc
    70501 ataaagtccc ttatccaaat ggctttttac atcatcttgt gccataaaaa ataccctcct
    70561 tcttctcttt gtctttctta cgattatagc atattcccaa taatacctaa aacatatgaa
    70621 aaagcggttg cttttgttca acgattcact taaaataaga atgaaaatca ttctcaatta
    70681 gaaaggtgga ttggatgcgc tacggacgac tagtagcatt attagttatt ttaagtagta
    70741 tttcattgtt catcggagta tcgaatgtac aattgtcaga actgctccag ttagatgggc
    70801 aaagcttaca aatcttgcta gtgactcgtt tgccacgaac gatttgtttg atccttgtgg
    70861 gagcgaccag tagtatttgt gggttgatca tgcaacattt gacccaaaat aaatttgttt
    70921 caccaacgac tgcgggcacg atggatagtg cgcgtttagg tattttagtg gctatgattt
    70981 tcctacctgg tgcatccttg attgtccgat ccttgacagc attttgcttt gcttttgcag
    71041 gaacattgct ttttttatcc cttacgcgat tgttcctcca gaaaaatcaa ttgatgcttc
    71101 cgttgattgg agtcatgtta gggaatatca ttggttcagt tgctactttt tttgcttatc
    71161 aattccagtt ggtacaaaat atgacttcat ggcttcaagg gaattttgcg actgtgatac
    71221 gaggaaatta tgagttattg tatgctgttg ttccattgtt gctcttggtt tggctatttg
    71281 cttaccgatt tacgatcatt ggtctgggcg aatcgtttgc agtaaatctg ggaatagatt
    71341 accagaagat gcagttttta ggcattggct tagtatcatt ggctagtgca gtcatgttga
    71401 tcatggtagg aagcattcct tttatcggag tgatcattcc gaatcttgtc tctagaatat
    71461 atggagatca agtgcacaaa acaattggcg taactgcttt agcaggtagt ttgttcttag
    71521 tattttgcga tcttgtcgct cgcgtgttga tttttcctta tgaaatacca gtcagtgtga
    71581 tcgttggaat catcggtggg gctatcttcc tttatcttct gataagggga cgacgatatg
    71641 cttaaaaaaa agccatctat ttttattggt atgcttgttc ttgttttatt tgttacagga
    71701 atgtacttga cgatccatac atatggaaac tggcattttg catgtgcatt gaggagtaag
    71761 aaaatttttg catttatcct tgtctcgcta gctgtcagca gtgccacaat cagctttcag
    71821 acaattacgc ataaccagtt tttgactcct tcagtattgg gacttgagtc gttatatgtc
    71881 cttgtccaaa cggtcctttt cttctttatc ggaggagtga cgatgcttca gcagacaggt
    71941 acagtttttt tcttattgaa cgttgggctg atggtcgggc tgagtctatt gttatttttc
    72001 tttcttttga aaaaaatgag agcgaatctt tttttattac tgatgtcagg gatgatttta
    72061 ggtacgcttt ttggtagtgt cagtaccttt ttacaagtca tcatggatcc gaatgaatat
    72121 gatttgcttc aaggcagatt atttgcaagc tttggcaata tccagagtca gtatcttttg
    72181 cctgcttctt tgatcattat cggctgctgg tacctgttat ttcgatatcg acatgtgcta
    72241 gacgttttcc atctcggaaa tgaacaagca gtaaacctcg ggatagacac agatcgtttt
    72301 gaatttttga ttttatgttg tgtcagtctt ttgacaggga catcaacggc tcttgtgggt
    72361 ccagttactt ttttagggtt tattgtcgca aatatcagtt atcgtgtctt ttctacttat
    72421 cgtcatggtc agctatttat cggaagcttt ctggtagcta tgctgtttct gacaggagga
    72481 cagctcgcag tagagcagat tttcggctgg aatacaacag tgagtgtcgt cgttgaattt
    72541 gtcggaggaa tttattttgt cacaaaattg attcgcgaaa ggaagagtat cctatgattg
    72601 atttacaaaa ggtcaataaa ctgtaccatg gaaaatcagt ggttaaagat gtacagttaa
    72661 cgattcctga aaagaaattg actgctttta tcggtccaaa tggtgctgga aagtcaacgg
    72721 tgttgtcgat gatcagtcgt ttgataccaa aagatacagg tgaaatctat ttagaccata
    72781 acgaagtaaa ggcttggaaa tccaatgaat tagccaaaaa attatcgatc ctacgacaat
    72841 cgaatgaaat aaatgtccaa atcaccgtga aagaattagt agaatttgga cgatttcctt
    72901 atacgaaagg aaaattgacg acaaaagacg aggaaatcgt tcaggaatca ctggaacacc
    72961 ttgggttaga agaaatagcg gatcaatcga ttgacactct ttcaggcggt caactccaac
    73021 gtgcgtatat tgcaatggtg ctagcgcaag acacggatta tattttactg gatgaacctt
    73081 tgaacaatct agatatgaat tttgcggttc aaatcatgca gattttaaaa aatttagtac
    73141 aagaaatcgg gaaaacaatt gttattgttc ttcatgatat caactttgct gcaagttatg
    73201 cagacgaaat tgttgcaatg aaagatggac agttatttac gcaaggaacg acccaagaaa
    73261 tgataaaaaa agatgtattg gatgaattat acgaaatgga catacggatt tgtgagattg
    73321 aaggccgcag attctgtctt tatttctaaa actaacatta ggagagaaga gatgaaaaaa
    73381 ttagtagtag gtatcttgat tctagcatca tttggtttag cagcatgtgg aaatagcaac
    73441 aacaccagtc aggcagacac aaaaaaaagc agcacacaaa ctgaaactac attgacgata
    73501 acagatagta atggagacca aatcgaggtg ccaaataatc cagaaaaagt cgtcgtattc
    73561 gacaatggtt ctttagatac gatggatgct cttggtgttg gagataaggt agttggagca
    73621 gctacaagca gccttcctga gtatctttca tcctataaaa aagtagaatc agcaggtgga
    73681 ataaaagaac cagacttaga aaaaattaat cagcttcagc cagatctaat catcatttct
    73741 ggccgacaaa gagattttca aaaagatcta agtcaaattg ctccgaccat tttcctttct
    73801 ttagatgcga aaaatccatg ggaatctttc cagcaaaatg tcactgcttt aggggagatc
    73861 tttggtaagc aagaagaagc taaaactcaa ttagaggaat tatctagtgc aatcgatcag
    73921 acgaaaaaga aagcagaagc tactgacaaa aaagcactag tcacacttgt gaatgaaggg
    73981 caattatcag cctacggatc tggttctaga ttcggtttca tccatgattt atttggcttt
    74041 gaacaagcag atgatcaaat cgaagcatcc actcacggtc aaagtgtttc ttatgaatat
    74101 gtgctggaaa aaaatccgga tatcttattt gtagtagaca gaacaaaagc tattggtgga
    74161 gacgacagca aagatgatat ttctgccaat gaactggtag cacaaacgaa tgctggtaaa
    74221 aatcagcaga tcatctccct tgaaccagat gtctggtatt taagcggggg cggtttagaa
    74281 tcaatgaaac tgatgatcga agatgtgaat caagcattta aatagcataa ataacagagg
    74341 attaaactct catactatgt aacaataatg tttcttacat tcttaaatag caagagccta
    74401 tcctcaaaga agttatcaaa aaccatccgt aaaatcgtat tgatgatttt acggatggtt
    74461 tttatttatt tgagaaatct tttttcaacg tgaattcatt atctacatca tacgggttga
    74521 ttctagaatg gggagtggca aaaaatagtg tgatccattt tttcagcatc aagatcgcct
    74581 cctttgattt gaatcaaagg tacaaagcga aacttagata aaacttaaat ttagatattt
    74641 ttattttgta ccgatctctt ctttaaacga tgttccatct gctgcttctc ggcaataagt
    74701 ccactagact agaaaatatg agaagctatt ttctgtccag cgttcttatt actcaaagca
    74761 aatcgcagtt gtcacaatct cttctaaacg atgttccatc tgctgcttct cggcaataag
    74821 cccactagac tagaaaatat gagaagctat tttctgtcca gcgttcttat tactcaaagc
    74881 aaatcgcagt tgtcacaatc tcttctaaac aatgttcaat cagctgacct tcggtattag
    74941 ctcaaagcac tgatttcaca acctctattt aggcatgtgt ctcagcaagc ggttgatagt
    75001 tgatgatctg catttttcga taaaaatcgg cgggaagctt actccaccgt tttaataatt
    75061 gatagaaata actctcttct gctttgattt tatactcgct taatatcaat ccaaaactat
    75121 tcgttttttt tgaagctaat tttttataaa tatccaatgc tttggtttta tcttcattga
    75181 caaagagtgt ttttacttca atataagaac cctcgatatt ttgtgtttct tcgataaaat
    75241 agatgttttt tatatattcc ttcatagtga ttcctccaaa gaagtctttt atttgataag
    75301 tttactctac cgaagagttg ttaataatcc gtgaacaaaa agtaattctt tttccaatag
    75361 ttgttaaagg ttttttattt tttatagtat tttttgttat ttaagaaagg aataaggtta
    75421 aacaaataac aggttgcttt ttgggtcttt ttccgttcaa gaaactgaac gaagaaaagg
    75481 gaaagttgtc tcttttgtga taagagaaat cataataaac gaatgactta ctttccaata
    75541 aattgagaac agtcagaacc agacaaatta gttgcatctg atgaataaag tgattattct
    75601 atgacatgag gtgatacaga tgaaattaga agtgacaaaa gaagcacaag aagtattgaa
    75661 gaataaatta gaagcagacg atcaactttt attagatatc gaaaacggtg acggtccatt
    75721 tgcagaaagc caagtcagtt gccaattgga tacggcattt cgattgatca ttgtcaaaaa
    75781 agatacacaa acagatctaa gtctttattc tgtagtggct gatacaccag ttggaccaat
    75841 caaaatcaaa gacagtgcaa tactttacat ggatgatcca tccacacttg ctttagaacc
    75901 aacttataat agtttacaat taaaaggacc aagcggcttg agaaaaggca acttgcaagt
    75961 tgttcgaaaa gactagaggg ataaaatggg aaaattggcg atcatcagcg acctacatgt
    76021 ggacatcaat aaattcggcg aatcagagct gatgcagctt tggcaagttt tgcaaaagca
    76081 caagatcact cggcttcatc tagccggaga tacggctaat aaagtagacc gttgcctagc
    76141 tgttgtcgaa tttttcgtca ataaaggatt atctacgaca tttcatttcg gcaatcacga
    76201 gttggcagat gtgcccaatg aacagatgat ggaccatttt ccagacccac attttttaaa
    76261 tgaacggtat attgctttaa atgagcagac cgttctttta gggatgaatg gctggtacga
    76321 ctatcagttt tccgaattga aagattacga ggcaatcgta cgaatgaagc gtttgtattg
    76381 gtatgatcgg ttcatccagc gtgggaaaac agatctcgag gtcaatgcag aaatgcttgc
    76441 agctgcaaaa aatttgctag accagctgga agcaaaaaaa cttgaggtga ttttatccac
    76501 tcatttcgtt cctaaacggg catttatcat gtatcaatct gctccttatg agcgttggaa
    76561 caaattaaat gcatttttag gatcagaatc attcggagaa ttacttgacc gttattcaaa
    76621 tatcaaacaa gtagtttttg gccatacgca tcgtcgcttt gaagataaat ccatcggtca
    76681 cacgatttac agctgcagac cttttggtta ttattatgaa tggcagctga cccgagattt
    76741 tgtcttgaaa gaacagttgc tagacagcta tgatcctatg aaattacgtg tcctcttgcg
    76801 tgcccatcac ccagcttttc aggcctatca aacacaacat ttgcaagagg aatttgaaca
    76861 agcgatgaca atcgttccgt attgagacag cattagtaat gtgtaaaaga aaataccttg
    76921 taaaacataa ggataagtaa gagcagtaga tccatttatc gtggatatgc tgcttttttt
    76981 gcggaaaata atcagcgaat gtttatttga tcggatgatt tgtaactcgc agagtactag
    77041 atgcaagcgt gtattctttt tttgaacgat aatcacaaaa aaaaatataa tatacatttt
    77101 ttatttaatt ataattaata ttgattgaag tctaggcagt caagtcacat tttgatagat
    77161 aggaggggga cttacgaatt attttaaagg ctatgacagc tgtatttcta gattgtgttt
    77221 gcatcaagtt gcaataaaaa ctagaaatca aatcgggctg ataatcattt ataactaatc
    77281 gatacatagg accaaatata ggtctatgtt cttttatttc ataagtatcg taaaacagaa
    77341 ttttgctatg gattaatcca atcgactgat atgtttagta aataaagaaa gaggaatgtc
    77401 aatgatgaaa aaagaaatcg tattaacagc agcaatgatg ttattcagca tggtagcaag
    77461 tacgactttt gtttctgcaa ctgaagtata cccaaaggaa tataacacag aaggaacgat
    77521 tacatttgaa gcaggagatg agggtgtaac tcctcctgtt gatccagaga atcctgatcc
    77581 caataaacct gtcgatccat cagatcctcc aagccctgga acaggagggg cattatcgat
    77641 tgattatgga tcaaaattca aatttgggac tcaaaaaatt tctactgcgg ataaaacgta
    77701 ttatgcagct gctgatgtga tgaacgatgg gtctagaaaa ccgacatatg tccaagtgac
    77761 agataggaga agtaccttat ctgggtggaa attaagtgtc tctcaaccag aacagtttaa
    77821 aactgccagt ggggatgaac tagttggagc acagctgaaa tttacgaaag ggcaggctgt
    77881 ttctttggtt gatccaacat atacgccgca aacggtgaat tctgaattaa cgttgacacc
    77941 gggaggaaac aatacgttag caattaatgc taaatcagga acaggtgtgg ggacttgggt
    78001 ctatcgattt ggtgcaaatg aaaatgaaaa tcaagatgcg gtacagcttt cggtacccgg
    78061 taaatctgtc aagttagcgc aacaatattc cactaaatta gtttggacat tagaagatac
    78121 acctaacaac tagtttactg aaaagaactg ttaaaaataa atacgtagtc atattgtctt
    78181 gcgaggtaat cgagcaaaaa ttgtcaaagg ctaggctaaa tttgtttgga tacgaatagt
    78241 tcatctagga atttaacgat caggaggaaa agatatattg aaaaagagga ggatcgtgct
    78301 cattttaagc agtctcttag tgactaatat ctttccgatc atagggtact ctttggaatt
    78361 gaatcagttg gagcatggaa atagaagaga cgaagaaagc aaactcactt cgacattcga
    78421 cagtttaata gaatccaacc agacacaaga aagcagtgag gatttaagta tttcgagtag
    78481 tggagagacc actgacaacg tagaagctga gggttcggac aaacaaggac tagaagatgc
    78541 accaatttct gcggttgagc tgcctgacga tcccaatatt atttctatcg ataaaatttt
    78601 tcaaacacct atcggtacta gcacgtccat attggaaaat gggaagttac tgcagttgaa
    78661 cccagcagaa aggtctcaaa gaggagctat ttggtcaaat aagaagatta gtttactctc
    78721 ggattttaca tttaaatctt atttatactt aggaaatcaa cgagatagag cgggagatgg
    78781 catgaccttt acattgactg gcgatcctag aatggctagt aatcccagtc aagttattgg
    78841 atcgccaggt atgggaattg gctcatattc tactcgttct ggccaacctt acattgcgaa
    78901 tgcgctctcg attgagtttg atacgtataa aaatagtggt tcttctgatc gaatggatcg
    78961 agagataact gctgataata gagggcatgg acatgtcgct tttgtcacgc ctaaaccaaa
    79021 taataataat tattctggag agcatactgg cgtgacggta gctcctacat ttctttctaa
    79081 tggcacatgg cgcttgttaa taattaaatg ggacgcggag caaagaagtc ttacttatga
    79141 tttggaaggt gtcggtcgct cttctcggat tatacctgat ctcattaggc agtttggttc
    79201 tacagaagta aattgggggt ttacttcttc aacaggtact ttttttcaag aaaatgctgt
    79261 cgctgtcacg cgacttccat cgaatgtcac atcaacagct gatgtaaaag tcaacgatag
    79321 tgattatggg gccatcgctg aagctggagg aaacgataag ataactatca gaaatacatt
    79381 gaaggtcgag ggggattttc ttgaatataa gaaaatgcag actagtatcg acctcccaca
    79441 agaattaact attcccgagc atatcttgat caatggaaat caaattgatg ccaaagatat
    79501 cttgataacc ggaaatcgag tcacgctaaa tctgacagat tattttattg ctggagatac
    79561 gttaataatc gaattggaga caactttgca ggaagtaatt cctgaaaaag tactcactat
    79621 gcattttgaa taccttgaaa acgggaattt gattgagcaa tcgaatgaaa taaacctaaa
    79681 gatcgcaaag ctgaaggaga tagatatcca tgtttattat aaagataaag acagtcagat
    79741 tgatattgct gaaatgaaaa caatcactgg gaaaatagga gagaagtata aagaaactcc
    79801 tattgatatt gaagggtatg tttttgactc ggattcagcg aatacagaag gcatcttctc
    79861 cgaaaataca gaggatatct atttttatta cagaaaaggc aaactttatc tctcaactgc
    79921 tcctcaatat gtcaattttg gaaaacataa aatatccagt agaccattaa gtgaattcgg
    79981 acagttttca ggtgggttga aaattataga tgaacgggct tttggaagtt ggaaattaca
    80041 attgaaacaa gagaaagttc ttacaaatgg agattttgag cttccagaag ctctttcctt
    80101 tgttacctct gaaaatacaa cggttatagg gacatccgct gttacgattt tcgaaagtga
    80161 tcaaaagggt gaaagcgatt tgtcctctct tttagattct acagctcatc gtggaatcag
    80221 aatagatatt cctgtagaaa accaaagaat cggaaccttt gaggggaaac tatcttggat
    80281 acttgcagat gtcccaggaa attaacagga agagaagaat ataacaaaat tattgagttt
    80341 caagaagggc acaggtacca taggctcttg tgtcccacag agacaagatt ttgttatagt
    80401 cttctcttac atatgattaa aggagcgatt ttcttgagac caaaacaaca attatttata
    80461 acactcctag ttattttagg ggcacttttt aacaatatat cagtgtcagc tagtgcattt
    80521 aatttttcag tgacgccgat aacttcagag aatcaaatcg acaagagaaa aacttacttc
    80581 gatcttcaac ttgatccgga tcaggaagta gaagtaaaag cggaattgag aaatgataca
    80641 gaaaaagaag tgaaaatcga tatttctgtg aatagcgcaa ccaccaattc aaatgtgatg
    80701 gttgaatacg gaaaaaacga gattgaaaaa gacatgagcc tgatctttga tctggtagat
    80761 tatgtgagct atccacagat agtgacgcta aaacctaaaa gtgtccaaac cgttgctttt
    80821 catgtcagga tgccgaatga gcgatttgat ggtgtattgg ctggtgggat cacattccaa
    80881 gaaatccaga cggaaaagga ccaaacagaa actaaggatc aaagcttatc gattgagaat
    80941 gaatacacct atattattgc tttgttgatg caacagacac tgaacgaagt tgctccgaac
    81001 cttactcttc atgaagttaa accagatcaa atcaatgcaa gaaatgtcat cttagcaaac
    81061 gttcaaaatg atcaaaaaac ctatatcaat caagtagtta ttgaaacaaa aattacaaaa
    81121 aaaggacatt ctgaagtttt atatcaagag gaaaaagaag gattgcagat tgcgccgaat
    81181 acaaattttt cttttccaac tgctttaaat ggtcaaccgc taaccccagg tgaatatcac
    81241 cttacaatga ctgttttagg aaatgaaaat gaaaatggaa aattttcgag aaaaaaagga
    81301 aatactacga tcaactatac caatcaatgg gtatttgaaa aagattttac aatagacgga
    81361 aaagtagcca aggaattgaa cacgacggat gttacgatta aaaaagacaa taattggatt
    81421 tatatcttag tagcttcact tttcttattg tttattttat tatgcatact tttgtttgtt
    81481 ttgagaaaaa aagaagagaa agaaaagtaa ggttaagata acaaatagta aatttttatt
    81541 attgtaggat ggttgggctc tttttataac aatataaaat ataccatttt attaataaaa
    81601 atgcatacta gtaaatgata taattctaat aacaggtttg atcgcgagaa aggccaaata
    81661 aaacgctctc caaaatgtta aaaaaaagac aggacgatgt atattataca aacaagaatc
    81721 gctgacaaca gtgactcgga tccaggaaat cagttttatc cccagctagt tggtgaatgc
    81781 atggaaagta atatacgtag aaagagctga tgactaatga attatgagtt ttttttaagc
    81841 aaagaagatc acagaagtta ttcattactt aaatatttag aagcaagctc aaatttatca
    81901 gagtcaatat cgaatgttca agaagagttg tcgctatcga catttctctt gaaaaaaacg
    81961 atcgataagc tgaaacatga tttacaaaag ttcgatctag agaaaaattt gcgagtaacc
    82021 gtcagtgaat tggatatatg tctggaaatt aatggaaaat gttctagcaa agctctgtta
    82081 tctcgttata taacggattc tttatctatg aaaatggtat tggctttctt tcaggaaaaa
    82141 tatatctcag tagaaaattt tgctaaacag catcacgtga gttattctgt ggcttataaa
    82201 gtattacaaa gactaaaaag aaatctaaaa aaatatcaaa ttttttttga aaagagaaag
    82261 tt
//
LOCUS       DASHRQ010000004        79519 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.004, whole genome shotgun sequence.
ACCESSION   DASHRQ010000004 DASHRQ010000000
VERSION     DASHRQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 79519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.004"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            61..1227
                     /locus_tag="U2M88_000292"
     CDS             61..1227
                     /locus_tag="U2M88_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEM8372704.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            1273..2769
                     /locus_tag="U2M88_000293"
     CDS             1273..2769
                     /locus_tag="U2M88_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEM8372705.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            2850..3533
                     /locus_tag="U2M88_000294"
     CDS             2850..3533
                     /locus_tag="U2M88_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEM8372706.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            4178..5368
                     /locus_tag="U2M88_000295"
     CDS             4178..5368
                     /locus_tag="U2M88_000295"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEM8372707.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            5470..6423
                     /locus_tag="U2M88_000296"
     CDS             5470..6423
                     /locus_tag="U2M88_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEM8372708.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            6492..7841
                     /locus_tag="U2M88_000297"
     CDS             6492..7841
                     /locus_tag="U2M88_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8372709.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(8024..8347)
                     /locus_tag="U2M88_000298"
     CDS             complement(8024..8347)
                     /locus_tag="U2M88_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372710.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            8476..9639
                     /locus_tag="U2M88_000299"
     CDS             8476..9639
                     /locus_tag="U2M88_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEM8372711.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            9709..11058
                     /locus_tag="U2M88_000300"
     CDS             9709..11058
                     /locus_tag="U2M88_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEM8372712.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(11103..11534)
                     /locus_tag="U2M88_000301"
     CDS             complement(11103..11534)
                     /locus_tag="U2M88_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8372713.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            11922..14564
                     /gene="alaS"
                     /locus_tag="U2M88_000302"
     CDS             11922..14564
                     /gene="alaS"
                     /locus_tag="U2M88_000302"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEM8372714.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            14764..15360
                     /locus_tag="U2M88_000303"
     CDS             14764..15360
                     /locus_tag="U2M88_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEM8372715.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            15482..16630
                     /locus_tag="U2M88_000304"
     CDS             15482..16630
                     /locus_tag="U2M88_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEM8372716.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(16668..17081)
                     /locus_tag="U2M88_000305"
     CDS             complement(16668..17081)
                     /locus_tag="U2M88_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEM8372717.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            17160..17591
                     /locus_tag="U2M88_000306"
     CDS             17160..17591
                     /locus_tag="U2M88_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEM8372718.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(17690..17923)
                     /locus_tag="U2M88_000307"
     CDS             complement(17690..17923)
                     /locus_tag="U2M88_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEM8372719.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            18106..19776
                     /locus_tag="U2M88_000308"
     CDS             18106..19776
                     /locus_tag="U2M88_000308"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEM8372720.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            19923..20309
                     /locus_tag="U2M88_000309"
     CDS             19923..20309
                     /locus_tag="U2M88_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372721.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            20296..20994
                     /locus_tag="U2M88_000310"
     CDS             20296..20994
                     /locus_tag="U2M88_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEM8372722.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            20999..21463
                     /locus_tag="U2M88_000311"
     CDS             20999..21463
                     /locus_tag="U2M88_000311"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEM8372723.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            21460..22371
                     /locus_tag="U2M88_000312"
     CDS             21460..22371
                     /locus_tag="U2M88_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEM8372724.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            22606..23145
                     /gene="pyrR"
                     /locus_tag="U2M88_000313"
     CDS             22606..23145
                     /gene="pyrR"
                     /locus_tag="U2M88_000313"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEM8372725.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            23159..24442
                     /locus_tag="U2M88_000314"
     CDS             23159..24442
                     /locus_tag="U2M88_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEM8372726.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            24455..25381
                     /locus_tag="U2M88_000315"
     CDS             24455..25381
                     /locus_tag="U2M88_000315"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEM8372727.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            25400..26680
                     /locus_tag="U2M88_000316"
     CDS             25400..26680
                     /locus_tag="U2M88_000316"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEM8372728.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            26685..27761
                     /gene="carA"
                     /locus_tag="U2M88_000317"
     CDS             26685..27761
                     /gene="carA"
                     /locus_tag="U2M88_000317"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEM8372729.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            27766..30951
                     /gene="carB"
                     /locus_tag="U2M88_000318"
     CDS             27766..30951
                     /gene="carB"
                     /locus_tag="U2M88_000318"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEM8372730.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            31005..31781
                     /locus_tag="U2M88_000319"
     CDS             31005..31781
                     /locus_tag="U2M88_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEM8372731.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            31778..32710
                     /locus_tag="U2M88_000320"
     CDS             31778..32710
                     /locus_tag="U2M88_000320"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEM8372732.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            32707..33423
                     /gene="pyrF"
                     /locus_tag="U2M88_000321"
     CDS             32707..33423
                     /gene="pyrF"
                     /locus_tag="U2M88_000321"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEM8372733.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            33430..34059
                     /locus_tag="U2M88_000322"
     CDS             33430..34059
                     /locus_tag="U2M88_000322"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEM8372734.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            34047..34361
                     /locus_tag="U2M88_000323"
     CDS             34047..34361
                     /locus_tag="U2M88_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372735.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            34399..35040
                     /locus_tag="U2M88_000324"
     CDS             34399..35040
                     /locus_tag="U2M88_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEM8372736.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            35083..35775
                     /locus_tag="U2M88_000325"
     CDS             35083..35775
                     /locus_tag="U2M88_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEM8372737.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            35772..36674
                     /locus_tag="U2M88_000326"
     CDS             35772..36674
                     /locus_tag="U2M88_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEM8372738.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(36832..38538)
                     /gene="efbA"
                     /locus_tag="U2M88_000327"
     CDS             complement(36832..38538)
                     /gene="efbA"
                     /locus_tag="U2M88_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEM8372739.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            38728..39351
                     /locus_tag="U2M88_000328"
     CDS             38728..39351
                     /locus_tag="U2M88_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372740.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVEMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            39801..40850
                     /locus_tag="U2M88_000329"
     CDS             39801..40850
                     /locus_tag="U2M88_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM8372741.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            40847..41728
                     /locus_tag="U2M88_000330"
     CDS             40847..41728
                     /locus_tag="U2M88_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8372742.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            41731..42501
                     /locus_tag="U2M88_000331"
     CDS             41731..42501
                     /locus_tag="U2M88_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8372743.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            42780..43634
                     /gene="aroE"
                     /locus_tag="U2M88_000332"
     CDS             42780..43634
                     /gene="aroE"
                     /locus_tag="U2M88_000332"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEM8372744.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            43812..44975
                     /locus_tag="U2M88_000333"
     CDS             43812..44975
                     /locus_tag="U2M88_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEM8372745.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            45092..45502
                     /locus_tag="U2M88_000334"
     CDS             45092..45502
                     /locus_tag="U2M88_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEM8372746.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            45676..47904
                     /gene="topB"
                     /locus_tag="U2M88_000335"
     CDS             45676..47904
                     /gene="topB"
                     /locus_tag="U2M88_000335"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEM8372747.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            48075..48359
                     /locus_tag="U2M88_000336"
     CDS             48075..48359
                     /locus_tag="U2M88_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372748.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            48519..48755
                     /locus_tag="U2M88_000337"
     CDS             48519..48755
                     /locus_tag="U2M88_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEM8372749.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            48752..49150
                     /locus_tag="U2M88_000338"
     CDS             48752..49150
                     /locus_tag="U2M88_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEM8372750.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(49817..50761)
                     /locus_tag="U2M88_000339"
     CDS             complement(49817..50761)
                     /locus_tag="U2M88_000339"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEM8372751.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(51057..52001)
                     /locus_tag="U2M88_000340"
     CDS             complement(51057..52001)
                     /locus_tag="U2M88_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8372752.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            52222..53436
                     /locus_tag="U2M88_000341"
     CDS             52222..53436
                     /locus_tag="U2M88_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM8372753.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            53458..55473
                     /locus_tag="U2M88_000342"
     CDS             53458..55473
                     /locus_tag="U2M88_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEM8372754.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(55556..56929)
                     /locus_tag="U2M88_000343"
     CDS             complement(55556..56929)
                     /locus_tag="U2M88_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEM8372755.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGENGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(56955..57239)
                     /locus_tag="U2M88_000344"
     CDS             complement(56955..57239)
                     /locus_tag="U2M88_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8372756.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(57241..59256)
                     /locus_tag="U2M88_000345"
     CDS             complement(57241..59256)
                     /locus_tag="U2M88_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEM8372757.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(59435..59740)
                     /locus_tag="U2M88_000346"
     CDS             complement(59435..59740)
                     /locus_tag="U2M88_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEM8372758.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(59829..60860)
                     /locus_tag="U2M88_000347"
     CDS             complement(59829..60860)
                     /locus_tag="U2M88_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEM8372759.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            61067..61942
                     /locus_tag="U2M88_000348"
     CDS             61067..61942
                     /locus_tag="U2M88_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEM8372760.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(62094..62936)
                     /locus_tag="U2M88_000349"
     CDS             complement(62094..62936)
                     /locus_tag="U2M88_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEM8372761.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(63028..64905)
                     /locus_tag="U2M88_000350"
     CDS             complement(63028..64905)
                     /locus_tag="U2M88_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEM8372762.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(64902..66323)
                     /locus_tag="U2M88_000351"
     CDS             complement(64902..66323)
                     /locus_tag="U2M88_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEM8372763.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(66326..69715)
                     /locus_tag="U2M88_000352"
     CDS             complement(66326..69715)
                     /locus_tag="U2M88_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEM8372764.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            69972..71363
                     /locus_tag="U2M88_000353"
     CDS             69972..71363
                     /locus_tag="U2M88_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8372765.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(71436..72083)
                     /locus_tag="U2M88_000354"
     CDS             complement(71436..72083)
                     /locus_tag="U2M88_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEM8372766.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(72091..72777)
                     /locus_tag="U2M88_000355"
     CDS             complement(72091..72777)
                     /locus_tag="U2M88_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8372767.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(72783..73856)
                     /locus_tag="U2M88_000356"
     CDS             complement(72783..73856)
                     /locus_tag="U2M88_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8372768.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            74078..74752
                     /locus_tag="U2M88_000357"
     CDS             74078..74752
                     /locus_tag="U2M88_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8372769.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            74757..76097
                     /locus_tag="U2M88_000358"
     CDS             74757..76097
                     /locus_tag="U2M88_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEM8372770.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(76246..77232)
                     /locus_tag="U2M88_000359"
     CDS             complement(76246..77232)
                     /locus_tag="U2M88_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEM8372771.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            77956..78987
                     /locus_tag="U2M88_000360"
     CDS             77956..78987
                     /locus_tag="U2M88_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEM8372772.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            79096..>79519
                     /locus_tag="U2M88_000361"
     CDS             79096..>79519
                     /locus_tag="U2M88_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEM8372773.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT        25200 a        14179 c        16106 g        24034 t
ORIGIN      
        1 taaaagtgtg gggtaggact gatgtcccac ctcctttttt tataaataag gagattgtgt
       61 atggaaaaaa acaggaagtt caacctcgtc gcaactgcgg caagcgggct agaagcactg
      121 gttggtaaag aattaagaga tcttggaatc gaatgccaag ttgagaacgg acgtgcacgt
      181 tttgaaggaa caatggaaac gatcgctaca gctaatttat ggttacgtac agcagatcga
      241 gtaaaaatcg tggttgggga attcgatgcg tatacgtttg atgaattgtt tgaaaacgta
      301 aaagcattgc cttgggaaga ttttcttcca ctagatgcag cttttcctgt agctggaaaa
      361 tcgatcaagt cgaaactata tagcacacct gattgccaag cgatcacaaa aaaagcaatc
      421 gttgaacgtc tccgtaccta ttatcaccgt ccagcttctg taccactgac ggaaactggc
      481 gcacattttc agttggaagt agctttgctg aaagaccatg tgatggtaac tctggatacg
      541 acaggaccaa gtttattcaa acgtggttat cgcttagaaa aaggtggagc accattgaaa
      601 gaaaacatgg cagcagcact tattatgttg acaaattggc gaaaagaccg tccattttat
      661 gatcctgtat gtggttcagg tacgctttgt attgaagcag ccttaatagg ccataatatc
      721 gcaccaggat tcaatcgtga gtttgcttgc gaatcttggg attggttcag tcaagaaatc
      781 atggaaaatg ttcgatcagc agccgaagaa aaagcagatt atgatattga attagatatt
      841 actggttcag atatcaatgg acggatgatt gaaattgcca aagccaatgc agaagaaatc
      901 ggattaggct cttcgattac ctttaagcaa caagcagtca aagacttcaa aacggataaa
      961 gaatacgggg tcatcgtggc taatccgcct tacggagaac gtctaggaga agaagaaact
     1021 gttcggaaat tatataaaga aatgggcgat gtattccgtc cattaaaaac atggagtaaa
     1081 tatatcttga ctagcgactt agcgtttgag gaattttacg gacaaaaagc aaccaaaaaa
     1141 agaaaattgt acaatggtgc attgcgtacg gatctattcc agtattgggg aactcgtccg
     1201 cctagaaaaa aagagaaaga cgaataatct tactaattga ttcattcaat tggatgtttg
     1261 gaggaatagg gaatgaaaga aaaagagttt ttacaagaag taaaagaaat caatcttcta
     1321 caacaagctt taggaatcct cgattgggat acacaaacag gtatgcctga aaaagctagt
     1381 gcataccgaa gtgaagtaga cagctatctt tacagcattt atttttcaaa aaaaatcggt
     1441 ccaaaaatca aagaagccat tcgctatttt tctgaacatc cggaagaatt gtcggaagtt
     1501 ggaactgccg tttttgccaa agtaaaagaa gagtatgatt tggaacatcg cgtaccggaa
     1561 gatcttatga gaaaatatac agctgccgtt tcttcagcac acggacaatg gcaaaaagct
     1621 agagaaacac aacaattttc tgattttcaa cacgcattga atcaaaatat tgaattaaca
     1681 aaacagttga taccttactg gagaaaagat gaaaagacga attatgatgt tttgctgaat
     1741 caatacgaac ccgggatgac tgtcgaaatc cttgataagg tatttgatca gcttaaagaa
     1801 ggaattcttt cgatccgccg tacgctccaa gaaaaaggga ctgaacctga aacagacttt
     1861 ttaaatcgga gaatgacgaa aaaacaacag aagagatttg tagtgaaagt aatcgaacaa
     1921 cttggttatg attttagtcg tggtcgttta gatgacactg tccacccatt tatgactgga
     1981 attaatccga atgatgcacg aatcacgaca cgatggaacg aaacagattt caaaatggct
     2041 gtttttggag tgatccacga ggctggtcat ggtatgtatg aacaagacat cgatgataaa
     2101 tatgcgtata caccggttta tgaaggtaca tcaatgggta tccatgaatc acaatcgttg
     2161 tttaacgaga tcattattgg aagtagtcgc agcttctgga aaaagcaata tcctttcttt
     2221 caagaatgtg cagaaggaac attcgatgat attccttttg acaaatttta tcgttcattg
     2281 aaccatacaa aatcaagttt ggttcgtatc gaggcagaca gtttgactta ccctctccat
     2341 atcatcattc gttatgaaat cgaaaaaatg attttcaatg aaggggtaga tgttgctgaa
     2401 ctacctgaaa tatggaatca aaaatatgaa gagtatctgg gaatacgacc aacaaatgat
     2461 gtggaaggca tcctacaaga cgttcattgg tcaggtgcaa gctttggtta ttttccctct
     2521 tatgcattag ggtacatgta tgcggcacaa ttacttcatt ctttgcagca agaaatagat
     2581 gtggaaaatg ttctagcgtc agacgattat tctccaatca aaaactggat gtcagagcat
     2641 atccatcaat atggtgcatc tagaaaacca aatcaattaa tcatggatgc aacacatgaa
     2701 ccgcttaatc cgcaatacct gatcgactat atgaaagacc tgtattttaa tgtatatcag
     2761 gtaaaataaa atacaattta caaagatagc tgaaattcat ctaatctata gatgttttca
     2821 gctatttact taagaaagaa gtggaacgaa tggataaatt acaagtgatc gctgattata
     2881 gtatggacca attagcgcat gatcaaacag gacatgggag cgatcatacc aaaagagttg
     2941 tcaagttagc tgaaaaaatt ttggagacgg aaccgcaggc agaccgcttt gtgacgcttg
     3001 cagcagctta cctacatgat acgattgatg ataaagtcgt taaaaatgag aacgaggcaa
     3061 agcaacagct tcgtgtattc cttcgctctt tgccaatagt agaggaacag atctcgatga
     3121 ttttttccat tatcgaaaat atgtcctttt ctaaaaatct ctctgaagcg gccgaactat
     3181 ctctggaagg taagatcgtt caagacgctg atcgtatcga agcattagga gcaatcggca
     3241 ttttgcgtac tgcttatttc ggcggtggac atgggcaccc gatttttgat cctgaactgt
     3301 atccgcaaac attcaaggac aaagaaaatt accgaaaagg cacgacagtg atcaatcatt
     3361 tttatgaaaa actgttcttg ctgtctgata aaatgaatac tgattacgga tatgaagaag
     3421 ccaaacgaag agaaaccttc atgcgtgaat tcttagaaga attttttctg gaatggtcta
     3481 ttgacgatga aaattttact ccagacaatt ggatcaaaaa taacaaaaaa tgatggaggt
     3541 tgtgacagaa gtggtcagct ccaagatatt tcggatcatt ttccgtaaaa gttacttctg
     3601 gaacactgtt taccaagagg tcgtgagcct gccgttcagc tccgagtcac aaggaacaat
     3661 ttcaaaaaat agcttctcgt gtttgatgaa tattgtaact tgtgattcgg aattggcagg
     3721 agaacaccgt ttattcggaa gaataggact gtgacaagac ttttgtcaca gtcctttttt
     3781 cgaattcaac aagtaaaact tttaagaaat attcagagag taaaattaaa aattgctgat
     3841 aaacgaagat ggaaatttta tgtattttta ttggtctatg caataaataa attaaatatt
     3901 cagaatacaa ttgattttaa cgaaagggct tctatcaatg gttgtgttgt atttatctgc
     3961 ccaggtagag taaaatagat cattatctaa aaggtaattg ttcatactat ataagtcatt
     4021 tgaaagtaaa aagatactgt aaataaaaaa tgtggttaaa taattctcat aatcagtgta
     4081 agcgttatca aaaatatagc atacttgctt tgtagatgaa tgacacgata tatgtttggt
     4141 atgttctgca agacagatga atatggaggt ggagacaatg tcttccaaag aactaacaat
     4201 gtatttagag aaagaattgg atcaactgaa agaaaagggg ttatacaata cgatcgacat
     4261 actagaaagt gaaaatggtg cttgtatcat cgtagacgga aaaaagatga tcaatctagc
     4321 atccaacaat tatcttggat ttgccaatcg cgaagaattg aaaaaagcat gtattgaagc
     4381 aaccgaaact tatggtgtag gagcaggagc agtccgaacg atcaatggtt cgctgaaaat
     4441 ccatcagcaa ttagaagaaa aaatcgcaga attcaaaggg acagaagcag caatcgcctt
     4501 tcaatctgga ttcaactgca atatgggcgc tatctctgca gtaatgacaa aagaagatgc
     4561 tatcttatcg gatgaattaa accatgcatc tattattgac ggttgccgct tatccggagc
     4621 aaaaatcatc cgaattaaac atcaagatat gaaagacctt gagaaaaaag caaaggaagc
     4681 gatagaaagt aagaaatata aaaaaatcat gtatatcaca gatggtgttt tctccatgga
     4741 tggagacatt gcacgattgc cggaaattat tccgattgtt gaaaaatatg gactgatcac
     4801 ctacgtcgat gatgcgcacg gctcaggcgt aactggaaaa ggtgcaggaa ccgtcaagca
     4861 ttttggatta agcgacaaaa tcgatatgca aatgggcacg ttatcgaaag ctataggtgt
     4921 tgttgggggt tatgtagctg gatcgaaaac actaatcgac tggttgaaag ctagaagtcg
     4981 gccattccta ttttctactt cgctaactcc aggtgcagct gcagctgcat tggcttcgat
     5041 tactttgatg caagaacatc cggaacttgt cgaaaaagtt tgggaaaatg ctaattattt
     5101 taaagaagaa ttgaaaaaag taggctataa catcggtatg tctgaaacac ctattactcc
     5161 agtcattttg ggagacgaaa aagtgactca gactttctct aagaagctga tcgaacatgg
     5221 gatttatgct aaaccaatcg tttatccaac tgtgcctctt gggacaggca ggatcagaaa
     5281 tatgccgact gcggaacata cgagggaaat gcttgatgaa gcagtagcag tttatcaaaa
     5341 aataggaaaa gaaatggaaa ttatttaact aaatttagtt ttttcgtcta tttaaaatta
     5401 gagaacatgt tcacgcatat attgttaatc atctgaagat agtagtaaaa aagatcaggg
     5461 aggatcatca tgaaaaaagt attggtgaca ggatgtttag gacaaattgg ttcagagttg
     5521 atcacacggc tacgtcaaga actaggcagt gaaaatgtca ttgcgacaga tatacgcatg
     5581 cctgaagtca ttgagaaaaa cgatatcttt gagatcctag atgtaatgga ttacgaaaaa
     5641 atgcgcagca tagcagaaca atatcaagtg gacacattga ttcatttggc tgctcttttg
     5701 tcagctgttg cagaagcaaa accacaatta gcctggaatt tgaacgtgac tgggctggtc
     5761 aatgcattgg aagtcgctag agaattccag ctgaaatttt ttacgccaag ttcaatcggc
     5821 gcgtttggtc caaatactcc aaaagatcat accccacaag atactcttca gcgtcctaca
     5881 accatgtatg gggtaacgaa ggtagctgga gaactactat gtgattatta ttatgtgaaa
     5941 tatggcgtag atactcgtgg tgtccgtttc cctggattga tttcttatgc aaccttgcca
     6001 ggaggcggta cgacagacta cgcagtcgat atttattatt cagcaatcaa agaaggaaag
     6061 tacactagtt tcattgatca aggtacatca atggatatga tgtatatgcc agatgcgatc
     6121 gatgccatta taaaactcat gaatgcagat cctgatcgat tggttcatcg aaatgccttc
     6181 aatatatcgg cgatgtcttt tgacccggaa gagatcaaag catctattca aaaaatcata
     6241 ccagattttg aaatggatta tgatgtggat cctgtacgtc agtcaatagc agaatcttgg
     6301 ccaaattcac tagatgtttc ttgtgcgcaa gaggaatggg gcttttcgcc gaaatacgac
     6361 ttagatgtga tgacaaagga tatgttggaa aaactgaaag aaaaattggt agagacggta
     6421 taaaacaaga accacatcca aaaagttttt tatacttttg ggattggcta tggtatgaaa
     6481 ggggacaagg gatggaagaa tatcaagcca cgccagtaaa agaagtaaaa atagaaaatg
     6541 aattaaaacg tacgatggga ttttttactg ctttatcgac tgttatgggc acagtgatcg
     6601 gtgcaggggt attttttaaa gccgcaagcg tagcagaagt tacaggatcc gccagtttgc
     6661 atatgttttc ttggtttctc ggtggtatga tttctgtctg tgcaggacta acaggagctg
     6721 aactggcagc tgctattcca gaaacaggag ggatgattaa atatatcgaa cggatttacg
     6781 gaaatacagc agctttttta ttgggctggg cgcaagtcgt gatttatttt ccagcaaatg
     6841 tagcagcctt gtcgatcatt ttcggtacac agttcgtcaa tctatttggt ctttcccagt
     6901 caatgattgt tcccgtagct gttacagctg ctgtatcgat cctgttgatc aattttttag
     6961 gttctaaagc tggaggggct tttcagtcga ttacgcttgt ctgcaagctt attcctttgt
     7021 ttgtgatcgt cattttcggt ttgttccgac aagaaggagt agacttccaa ctgtttccga
     7081 tccaagcagg agaaaatctt tcatttttct cagccttggg agcgggcttg ctagcaacga
     7141 tgttcgcata cgacggttgg atacacgtag gaaatatttc gggagaattg aaaaagccgg
     7201 cgaaagattt gccaaaagca atttctttag gaatcatcgg aatcatgatc gtttatttat
     7261 tagtaaatgc ggtgttctta agaaccgcat ctatcgacgg agtcgcagga aacagcaatg
     7321 cggcaagtga cgttgcaaaa atgatttttg gcggcttcgg tggaagactg gtcacagttg
     7381 gtatcttgat ttctgtttat ggaaccatca atggctatac ccttactgga atgcgtctcc
     7441 cttatgttat ggcaaaagaa aacaacctgc cttttagcaa gctctttgct aaacttcatg
     7501 ataaaactaa agttcctgta gccgcaggta tcttagagct agtcatcgct attggcatga
     7561 tgatgatcgg tggttttgat acactaacgg atatgctgat ctttgtcatt tggatttttt
     7621 atacgatggt ctttgttggt gtcattcttc ttcgtaaaaa agaacctgat ttgtttcgac
     7681 catacaaagt tccaatgtat ccgtttattc cactagtagc aatcattgga gggactttta
     7741 tcgtatcaag cacgcttatc actcagacct ttttagcttc gatggggata gtattgacac
     7801 ttgctggtat cccgatttat ctctatttaa aacgtagata gctaaaacat tcgaatttta
     7861 ttagaactgc acttctctgc caaacaaaat attttattta aatgaggagt acgtggaaga
     7921 aatgtcaaaa gtactcgtta attttgatca cgtaaatgtc gttttgtcta cgatgaaata
     7981 gcaaagtaaa agacagaaat cgggctcaac cgatttctgt cttttacttt gatttgcgta
     8041 actgaactat tcggcgtacg ccgcgataaa tatttaataa aaccaagcaa ccagaaatga
     8101 aaatgggaag ccaaatcaga tagacagggt agccaagcac tttagacaaa gcatgtggag
     8161 tcattttatc catttcaaga agtgcttgct gccaagtctg aagattgaag actagcatga
     8221 aacccgaaat aatcaggaga ataaatccta aataatgaat tgtagttctt tttcttacat
     8281 aagcgatcgt agcataaagg aacagtaaaa aaataggtac gccaaaaaca aagtacatat
     8341 tcagcatctc gacaccccct ttgtgtttat cttactttac tttgaaagaa atgagaaata
     8401 atagcctcgt tacttgcttg aaacagcaaa ggactgctaa gataagagag atacaaagaa
     8461 tcaaggagga gtcatatggc aagttatatc gaaccgattc gttttgcgct gattgttttt
     8521 ccatttttag ccttagcgat ctcatctgtt ttttttattt atgaatatcg aaagtatgga
     8581 acatttctag taacaagggc agttattttg tattctttcg ttttttattt attgtgtgcg
     8641 tattttttgg tgatcttacc acttccggca aaggagacgg ttgcccaaat gacagggcca
     8701 aagatggagc tgcatttatt tgcttcatgg caacatttca gagacaacac ggtattggtc
     8761 ttgtctgaac ctagtaccta cctgccggca atgaagcaga gtgtattttt ggaaccagct
     8821 tttaatgttt tattactcat accatttggc atttatttaa gatattattt ccggctttct
     8881 tttatgaaaa cgatgattgc tagtttctgc ctttctctat tttttgaatt gacgcagcta
     8941 agtggacttt attttatcta cccaagacca taccgcttat ttgatgtgaa tgatttagtg
     9001 acaaatacat tgggtggaat cattggtttt gtcgttacgc cgttatttac atttatgctt
     9061 cctacaagaa gacgaatgga tgaactttct tatgaaaaag gaaagactgt ctcattgact
     9121 cgacgcttgg tcgcttattt gatcgactgg acatttttat ctcttattaa tttggtagcg
     9181 gcgattttct ttaaaattgc gttcgattca ggggcagtta cttctcatat ctggtggctt
     9241 ttattagaag taggggtata ctttatgatt gtaccgttta taaccaatgg atggacactt
     9301 ggcaaaaaaa ttgtgcgtat ccgtttggtg gaagaagacc gcgaacgaat ttctttaaaa
     9361 gcattgatga tccgctacgg gtatctgtac tttctttttt atggtttaag ctggttgggg
     9421 cagttgcttc agcaaatcat gaattcgaca acgaatacgg tgttgcagat gagtgcagta
     9481 ttcatttctc tcggtgtctt gttactacaa ggtattttta ttttaaatat cttgctatca
     9541 tttttgagaa aaaaacggat tctgttttat gaaaaagcta gtcacacata tactgttagc
     9601 acgattggcc aaacaaagga tgatgattct cgaaattagc tgagcaaaaa cgctttgtct
     9661 gtgttataat gccaaagtcg tgataattta aagagaagag gtgcacgaat gaataatttt
     9721 gatatattcc gcttcaaacc gtttatcaat gaagcgttaa ccgcaaaggg cttcaaacag
     9781 cccacagagg tccaggaacg tttgattcct ttaattgcaa aaggaaaaaa tatcattggg
     9841 caatctcaaa ctggtacagg aaaaacacat acgtttttgt tgcctttgtt tgaaaaaatc
     9901 aatgcagaaa aagaggaagt acaagttgtt attacagctc caagtaggga actagctgca
     9961 cagatctatg aagcagctac acaaatcgct aaattttcta caccggagat ccgtgtctcg
    10021 aattttgtcg gcggtacaga caaacagcgt caattaaatc gtctgcatca ccaacagcca
    10081 caggttgtag taggcacgcc tggtagaata ttagacatga tgaatgaaca agcgctgaag
    10141 attcacaccg catttgcatt tgttgtcgat gaagcggata tgacattgga tatgggtttt
    10201 ttaaatgaag tagaccaaat tgcaagtcga ttaccagaaa agctgcagtt tttagttttt
    10261 tctgccacta ttccagaaaa acttcgtcca ttcttacgaa aatacatgga aaatccaatc
    10321 attgaagaaa tcaaaccaaa agcagttatt tctgaagaca ttgaaaattg gctgatttca
    10381 acaaaaggta aagatgagaa tcaaatgatt tatcaactgc tgacaattgg tcatccttat
    10441 ttagcaatcg tatttgccaa tacaaaacag catgtggatg aaattgccga ttacttgaaa
    10501 aaccaaggct tgaaagtagc aaagattcat ggagacatca ccccaagaga gagaaaacga
    10561 gtgatgcgac aagtacagaa tctggagtat caatatgttg tagcgactga tttagctgct
    10621 agagggatcg atatcgaagg tgtctctcat gttattaatg cagaaattcc agaagatcta
    10681 gacttcttta tccatcgtgt cggacgtact ggacgtaatg gcttaaaagg aacggccatt
    10741 acgttatatt cgccaaatga tgagcaagcc attgatgcga tagaaaaatt gggcgtctca
    10801 tttgaaccaa aagaaatcaa aaacggggaa atcgtcaaaa catacgatcg taatagacgt
    10861 gccaaacgag aaaaatcaaa agatgtatta gatcccacgc tgattggtct tgtgaagaag
    10921 aaaaagaaaa agatcaagcc tggttataag aaaaaaatcg attgggcaat tgcagaaaaa
    10981 aataaaaaag aacgcaaaat cgaacgcaga cagcaaagcc gtactgctcg taaaaataag
    11041 aaaaattcac ataaataaaa aacaagaggt tgtgacaaaa ggcgtgtcac aatctcttgt
    11101 ttttaagcta gacttttttt cattccgata caaagtactt catcttcaat aaaaggttct
    11161 gtgcaagtat gatacccttg tttttcataa aaatctacag ccgtcatttc agctactaca
    11221 taactagtct gacaaccttc tgaaaccgcc cgtttttcag cagcttcaag caactgaccg
    11281 cctaaccctt ttcctcggta ttcttttgct acgcaaaatc gttccggctt gaagcaagta
    11341 ggtgttttct tttcataacg caaagtagct actggtcttt tttgatcaag aaatagataa
    11401 taggtttgtt gcgggatatc ttttccgtcg aattctagtt ctgcaggaat cccttgttct
    11461 aatacaaaaa cttgatatct caaaaagcag ctagctgcgc gtatccattc atccgctcca
    11521 actatttttt tcatcatatt cctccttgtc atgaatagca gagatgtatt gattccttta
    11581 ttcttatcgt accaaaaatc gtggagtatg accaaaagga aatgaaaata aaaattgaga
    11641 tattgacagt gatcaataga atgcgtatac taaataagta cgaaaaggat atgatttgtt
    11701 ttttaagact cacagaaaat tctttcttgg ctgaaaaaag aatagtcact aatcaaattg
    11761 ctcccttttt ctattttggt agaaataccg acgcagctca gcgttatcga gtttaagagg
    11821 taatgattac acatcattgc aatcaaggtg gtaccgcgag ttttcgtcct tggctgtgat
    11881 ggtgtttttt tgtatttaat ttatagaaag caggtaatga catgaaacaa ttaagtagtg
    11941 cacaagttcg acaaatgttt cttgatttct tccaatctaa aggacatacg attgaaccta
    12001 gtgcgtcttt gatccccgtg aacgatccaa ccttattgtg gatcaactct ggtgttgcaa
    12061 cattgaaaaa atattttgat ggttcagttg tgccagaaaa tccaagaatc acaaatgccc
    12121 aaaaatctat tcgaacaaat gatatcgaaa atgtcggtaa aacagcacgg caccatacga
    12181 tgtttgagat gctgggtaat ttttcgattg gtgactattt caaaaatgaa gccatccact
    12241 gggcatggga atttttgact gatccaaaat ggatggcatt cgatccagaa aaactttatg
    12301 taacagttta tccaaaagat acagaagcaa aacgcatctg gcatgaagaa gttggcctgc
    12361 cactagatca cattattgat gtagaagaca acttctggga tatcggtgca ggcccatgtg
    12421 gtccggatac ggaaattttc tatgaccgtg gagaagctta taacgattta gcagaagacg
    12481 atcctgaaaa ctatccagga ggagaaaatg aacgttattt agaaatctgg aacttagtat
    12541 tttcagaatt caaccataag cctgataaca catttgaacc acttcctcat aaaaacattg
    12601 atacggggat gggattggaa cgcatggtct caatcgttca agatgcgtca acgaactttg
    12661 agacagactt gtttatgcct attatccacg ccgtagaaaa gctaagcaaa caagtaaaat
    12721 atggtgaaaa cccagtaaca gatatttctt acaaagtaat cgcagatcat attcgcgccc
    12781 tttcatttgc tattggcgac ggtgcattgc catcgaatga agggagaggg tatgtcttac
    12841 gccgtttgct tcgtcgtgct gtcatgcatg ggaaaaaatt aggtattgaa caagcattct
    12901 tatatcgttt agtccctgtt gttggggaaa tcatggtaag ttattatcct gaagtacttc
    12961 aacaaaaaga atttattgaa aaagttatcc gtacagaaga agaacgtttc aatgaaacaa
    13021 tcaatgaagg tttggaaatc ttaaatcaag tgatccaaga agtaaaaaac gaaaacggga
    13081 cgactctagc aggaaaagac atcttcaaac tatacgacac atatggcttc ccggtagaat
    13141 taacggaaga ggtggcagaa gatgaaggac tgaaagttga tcatgctggt tttgaaaaag
    13201 aaatggatgc acaacgcgaa cgtgctcgct ctgcccgcag tactgaaaaa tcaatggggg
    13261 tacaatctgc tttattgaca gatatcaaag tagaaagtaa attcattgga tacacagaac
    13321 aagaatcaga tagtcaatta ttggtcatcg ttaaaggtga gtctcttttg aatgaagtcg
    13381 cagaaggtga agctcaaatg atctttgatc aaacaccttt ctatgcagaa atgggtgggc
    13441 aagtagcgga tcaaggatgg atcatgaatg aagtaggcga aatcgtagca gttgttacag
    13501 atgttcaaaa agcgcctaat ggtcaattta tgcacaccgt agaagtaaaa gcaccaatga
    13561 gtgaaggaca aacctatcat ctattgatcg atcaatcgat gcgcaatcgg atcatcaaaa
    13621 accatacagc aacccatcta ctgcacaagg cattgaaaga agtacttggc gagcatgcaa
    13681 atcaagctgg ttctttagtc gctcctggac atttacgatt cgactttacc cactttggtc
    13741 aaatcacttc agatgaatta gctgaaatgg aaagaatcgt caacgaaaaa atctgggaag
    13801 ctatcccagt tgaaacaatt gaaacagata tcgatacagc caaaaacatg ggtgcaatgg
    13861 ctttgtttgg tgaaaaatac ggacatgacg ttcgagtagt aaatattggc ggctggtcaa
    13921 tcgaactgtg cggaggcaca catgtagcaa atacagaaaa catcggaatc ttcaaaatcg
    13981 tttctgaatc aggaattggt gcaggcgtgc gacgaatcga ggctgtaaca agtaaagaag
    14041 cgtacgaact acttgaagaa gaagaaaaac aactaaaaca agtagcaaca attgttaaat
    14101 cacctcaatt gaaagaagtt gtttcaaaag cggaacaatt gcaacagcaa gtaagagagc
    14161 tacaaaagga aaatgaacaa ttagctagca agttagcgaa tcaacaagct ggtgatgtat
    14221 tcaaagatcc agaaacgatc aatggaaaaa caatcattgc agcacaagta aaagtgaaag
    14281 atatgaatca gctgcgtcaa ttagctgacc aatggaaaca aaaaaatatt tcagatattc
    14341 ttgttttagc aaacgaacaa gaaggaaaag tcagtctgct agcggcaatg acgaaagaat
    14401 caaatgaagc tggactaaaa gctggcgact tgattaaagc aatcgcaccg aaagtaggcg
    14461 gtggcggtgg tggacgtcca gacatggcac aagctggcgg gaaaaatcct gctggtattc
    14521 cagatgcact taaagctgcg aaagaatggg ttgcgcaaca atagggcgtt tacgtttgac
    14581 agaaaaacaa aagttcaaac attgaacgaa acatgattat gggggcgaag acaaacaagt
    14641 cttgccctca tacttttttc aattggatgg cgcatctaat caaatggtcg gattcggatg
    14701 gtctttagtt gacgaaagtg acagaaattt ttacgattag tataaataaa aaggggtgaa
    14761 aaaatgaata gtagaaataa gacctttcga ctagtcttac gtgcgatctt gttagcaatc
    14821 atcatcgtac aagcgatggt tccttggctg gggtttattc cattaggatt cattagttta
    14881 acaattatcc atatcacagt aattattgct gcagtagtat taggcccaaa agatggcatg
    14941 gtgattggct tgttttgggg aatcgctaca attgttcgtg cctatgccat gccaacaacg
    15001 ccatttgata cattagtgtt cacgaatccg attatctcgg tagttcccag agtacttgtt
    15061 ggcttagtag caggcttggt tttccactgg atttatcaaa aaaataaatc aataacgatc
    15121 agctcagcct ttgcaggggt actgggttca ttggtcaata cagtcttagt gttaggattc
    15181 atgggattat tttatacagg tgcaacggca gatgcctatg gtgtagactc tgctctttta
    15241 tttaaaacct tagcaggggt tgcagcaatc aatgggatac cagaagcaat aggtgcggga
    15301 atcataacac cattactagc aaaagcgtta tttgcagcaa caccgttgaa accagaataa
    15361 tgaccatctt ttaatagtaa taggacgcac gatagataga aaaaggaaga gaggagagga
    15421 aaacaatcgt cctctccatc tttttacctg agcaacaagc aaactagaag gggtaaaaaa
    15481 cttggctaag aaacaagaag aaaaaaagaa acaaataccg caacagcgga caaaaaaagg
    15541 agtactagcg ggtatcttag gattggttac gaatctgatc ttgtttgtct ctaagctaat
    15601 gatcgggctt ttttcaggaa gtgtctccat tatggctgat gcagtgaaca gtctatcaga
    15661 tactgcttca tccatattga ctttgatcgg atttaaaatt gctgcaaagc cagcagatca
    15721 ggaacatcct tatggacacg aacgcttcga atatatcagt gggttatttg tttcgatcat
    15781 cattacttat gtaggatttc aatttttaga gtcttctgct gaaaaaatca tccatccaga
    15841 aaatgtctct ttaacaccca tcgtcttttt tgtcctaatc atttccatct ttttgaaatt
    15901 tgcacaaggc aaaatgtatc aggttattgc gcaatccatt gattctgaaa cattgagagc
    15961 tacagcaaca gatagttata atgatgtctt tactacactg gctgtattgc tttcagcagc
    16021 aatcgaatgg gtaactggat ggcgtattga tggatatata ggttttgctt tagcattata
    16081 tatcctttat agtggattga cgatgcttcg aaattttgta tacgaactat taggaagtcg
    16141 cccaacgcca gcagagattg aagaaatgga agagcattta aatgcttgcg atgatatact
    16201 tggctatcat gatttattgg tgcataacta tggacccaat aaacgctttg catcagtcca
    16261 catcgaagta gatgaatcaa tgaatttgaa tgtggctcat aagattattg atctgataga
    16321 aaaagacttt aaaaagtctt tggatgtgga acttgtctgt cacttggatc caatgcctgt
    16381 tgataacgaa aaatactata atatcttgcg gcagttaaag caaattcttc ttgaaacagg
    16441 tacagggctt aatattcatg attttagagt aattcatcaa ggaaaaacgc tgcaatttga
    16501 tgtagtggtc cctgagaaat tccagtttgt tgatacagaa ctagaaagaa tcttacgtag
    16561 tcaaatagaa gaaaaaatcg gagattacca attggaaatt acgtttgatc acaattatct
    16621 tttatattaa atgagaaact ataaaaagac tccttaaagt ttttaattta aagagtcttt
    16681 ttttctttta attgtttatg gagagcctga cgtgcaagat tatctgctcc tttatttttg
    16741 ttttcgggca accattgcag gatcaataaa gaaaattcgg atagaagttc ttggattctt
    16801 tgaaggtagg gaaggaagag ctcgttcttc gttgcgtttt tgtcgaccgt agaaactagt
    16861 gttttgctgt ctgaaaaaca gaaaatcgtt tcattctgta agtcgttttt ctttagaaat
    16921 tctaaagttt ttaaaaagac ttcgaactct gcttgatggt ttgtccctat agaaagcggg
    16981 atagatagct gttcttgttt accttgatag acaatcaaga ttccacctcc actaggacca
    17041 gggttgcctt ttgtagaggc atcaatgtat acttttaaca taaaaagaga ttctcctttt
    17101 tgttcaaact aatgttatgg taagattata tcattactga atcggtgaga ggttagagaa
    17161 tgaaaaaaaa tattcgatgg cagcctgaac tggcacatac gatcatctat tggtcattga
    17221 ccttgatcat tctgtttttt agtctgattc tttctttaga aaacacaggt ccttattgga
    17281 aaagcaattt tgttttaggt atttttcttg tttttctatt tctaggtttg cggcgttctt
    17341 ttattttaaa atcaaactct gtgattatac gttatgctgc tttttggaaa gaccgagaaa
    17401 tatctgtcgg acagattcga tctttgacgc caactaatac tggggtgcaa atgatgttgc
    17461 aagatgagaa agccccatct gttttcatga tgcgaaaaaa agatcaagta gtcctttttg
    17521 atcattttga aaaaaacgga cccaaatcag aatcaattcc ttatccttat acgaaaaaaa
    17581 gaaaaagcta attttaaaaa tcagaaccta aaaaaacgaa tttttacagg aaaagaaaag
    17641 agtgagacgt acggttcatc ccgtttagcg tctcactcta tttgtttatc tattcatgca
    17701 tactagtatc ggtagtctga acgttgcctt tcttgacatg ggcagcttgt gttcctcgat
    17761 tcccttcgat gatctcaaat tcgaccactt gattttcttc caaagttttg aatccctctt
    17821 cttcaatcgc agtaaaatgt acaaaaattt cttctgtgtc atcataactg ataaagccgt
    17881 atcctttttt gttgtcaaac cattttacaa ttcctgtggt caaagcactc acgacctttc
    17941 taaagtctga tcatagcctt cattataaag agtatttata agtacgtcaa ataaattaaa
    18001 aacctgtaat tttatgtttt ttttctgaat aaaaaaaggt ataattggca tatcttaaca
    18061 aagaagatat tttgttgaat aaagttcgtg aaaaggggta atcaaatgaa gaatgatctg
    18121 caaatttcac aagaaacaga gctagaaccg attgttgaag tggctagtaa attagatctt
    18181 aaggaagacg atttggaact gtatgggaaa tataaggcga aaatcaattt ttctggaatc
    18241 aatcgatcaa aggataacgc tgaggggcat ttgatcttag ttacttccat caatccaaca
    18301 ccagctggcg aaggaaagtc aacgatcact gtagggttag gagacgcgct caataaaatc
    18361 aataaaaaat ctgttattgc attgcgagaa ccctcacttg gtcctgtgat gggaatcaaa
    18421 ggtggtgctg caggcggagg atatgcacag gtattgccta tggaagatat caacttgcat
    18481 tttactggtg acatgcatgc tatcacaaca gctaataatg cattatctgc tcttttagat
    18541 aatcatattc atcaaggaaa tgaattgaac atcgatgccc gccgagtgat ttggaaacgg
    18601 gtagtcgatc tcaatgatcg tgaactgcgt aaagtaattg ttggcttggg tgggccaatc
    18661 caaggtgttc caagagaaga tgggtttgat atcacagttg ccagcgaaat catggctatc
    18721 ttgtgtcttg cggcagacct tgaagattta aaggctcgtc ttgcaaggat cgttgtcggc
    18781 tatacatttg accgtcagcc tgtgactgct ggagacttga aagcagaagg cgctctggca
    18841 ttactgctga aagatgcaat caagccgaat cttgtacaaa caatttacgg aacaccagct
    18901 tttgtccacg ggggaccgtt tgccaatatc gctcatggct gcaatagtat tttagctacc
    18961 aagacagcat tgagattagc agattacgtt gtcacagagg caggatttgg tgcagatctt
    19021 ggcggagaga aatttttaga tatcaaagta cctaacttga aaaaagcgcc ggatgctgtg
    19081 gtgattgtcg ccactgtccg tgcattgaaa atgcatggcg gaatgaaaaa agatgaactt
    19141 aaaaatgaga atcttgatgc tttgaaagta ggttttgcaa atttaaagcg ccatatacgt
    19201 aatatggaac aatatcagct acctgtgatc gtcgcaatca atgaattcgt aacagataca
    19261 gacaatgaac tcacactgtt ggagcacttg tgtgaaaatc aggggattct tgccaagaga
    19321 gcgagtgtat gggcagatgg agcagaaggc ggcgttgatt tagcaaaagc tgtagtacga
    19381 ttgattgata gaaaagaagc taattacaag ccattatacc gcctagaaga aacgatacaa
    19441 gaaaagacag aaacaatcgt caagaaaatt tacggaggaa atggtgttgt tttcagcaaa
    19501 aaagcgcaaa aacaaataga agagtttacg aagaatggat gggacaaact gccgatttgt
    19561 atggcaaaaa cacagtattc attttctgat gatccttcac tgctaggtgc accggaagat
    19621 ttcaccatca cgatccgcga aatcatccca aaattagggg caggattttt agtagccttg
    19681 actggagatg ttatgacgat gcctggtttg cctaaaaaac ctgctgcatt gaatatggac
    19741 gtgacaaatg acggagaagt gttgggattg ttttaagctt gattataatc taccatcgaa
    19801 ggagctgtat tttgactaca gcttcttttt ttagacgata gggaattaat tttgtttaca
    19861 ctaggatcat tctttttctt tattgtattt tgagctatac taaagcataa taggagggta
    19921 tgatgaaaac gacatttata agaaaaaccg gtttttacgg tatgggtagc tcgattcagc
    19981 taagaatcaa caatgaaaaa cgcagtgttc tcagtcatga acaaaagata acgcttgatc
    20041 tggaagtgcc ctttacgatg caggtcacct tttattggct aaaaagcccc gtttatacag
    20101 tgaaagaagc aaagcctgct tatgtgatca caatgaatta cttgattctt caaatctatc
    20161 cgtttttatt tctagcaact gggttggggg ccgttttcct acaaaattta tttttcagtt
    20221 tttttgcagt tattgtgttg attgcgttct ttttttatat aaaaaaccgg gcatatttga
    20281 tcaaggagga aaattatggc aaactctgat ctttttttaa tcagtttcaa tcgaatcgaa
    20341 aaatggatga gagacgagat gggaaatgca cgaaatatgg gatttaccga acttgtcaga
    20401 cgcctagctc aaagaaaaca attaatgatc agaaaatacg aagatgatct tttacagctt
    20461 gctcagttaa ggaatgcgat cgtccatgat cggatcgcag tcgattttgt cattgcagag
    20521 ccaaatgatt ggacagtcaa gcgaatccag ctgatcgaac aagaactgat tcagcctgaa
    20581 accgtgctgc ctcgatttgc aaaacgtgta acaggttttg aacaagatct tccgttactt
    20641 gaactgttaa agatagtagc agaaaaaagg tattcacaat ttccgttata caataaagga
    20701 aaatttgtag cattgattac tttacgtaat atcgggtttt ggctagcaaa agaaagccaa
    20761 aaaggtccgg ttgatttaac aaataaacga gcgttggatt tgatcatcca aaacggaaag
    20821 tacacaaatt atcattttgt tccagctaaa acacatattt acgaggttga agcgatgttt
    20881 cgtgagcaag ggactttgga agccgtactt atcactaaag atgggaatcc cgatggtaat
    20941 ctgctcggga tcgtacgacc tcgtgatatt tataaacaaa tagaaaagga ttgatttttt
    21001 gttaattatt tattggatat tcagcgcttt gctagtagga gtggatcagt tggtcaaatg
    21061 gtggatcact gataatctag cgcttggaga gacgaaaaat cttataccag gtattttgtc
    21121 actgaatcat attcgaaaca ctggagccgc atggagtatg ctagaaggga agatgtggtt
    21181 ctttactata gtgacactca ttgcagtcgt tgtgattctc acattgatga ttaaaaatcg
    21241 tgaaaaaggt aatcgctggt tcatgattgg attgagtttg attctagctg gtgcgattgg
    21301 caattttatc gaccgtattc gtcttggcta tgtagtagat atgttccaga cagattttat
    21361 gagctttccg atttttaacg tagcagatgt gacacttgta tgtggagtga tttgtatgtt
    21421 aatttatatt attttagatg aaaaggatca aagaaaaaaa tgacagaact taaaacgacg
    21481 attcaccatg aatctggtag gatcgataaa gtattgacta atttattcac cgattacagt
    21541 cgttcgcaaa ttcagctgtg gctgaaaaat ggagcggttt ctgtcaatgg acagccagtc
    21601 aaagcaaatt acaaagtaaa acaaggagac gagattactg tcttgatccc cgaaccagaa
    21661 acattagatg tggtcccaga aaatatcccg ttgacgatcg tttacgaaga tgaagatgta
    21721 gcggtgataa acaaacctca aggaatggtc gttcatcctt cagcaggtca tacgcatggc
    21781 acaatggtca atgcgctgat gtaccatatc aaggatcttt ctacgatcaa cggcgttatt
    21841 cgtccaggta ttgttcaccg aatcgacaaa gatacttccg gactgctaat ggttgccaaa
    21901 aatgaccagg cacacgaatc attagctcag caattaaaag ataagacctc tttgcgcaaa
    21961 tatgttgctt tagtccatgg agagattcct cacgaaaaag gaaccatcaa tgcgccaatc
    22021 ggccgttcga aggaaaaccg gaaaatgcag gcgataagag aagatgggaa gtcggctgtc
    22081 acacatttta ctgtattgga acgtttcgaa ggatttactc tggttgaatt aaaactagaa
    22141 accggaagaa cacaccagat ccgtgtgcat atgaagtata tcggttatcc gttagctgga
    22201 gatccagtat acggaccgaa gaaaactttg aaaggcaatg gtcagtttct ccatgcgcaa
    22261 atgttaggtt ttacgcaccc gactacaaac gaaaagatga tctttgaagc acctcttcca
    22321 gaagtttttg aaaaaacttt agaaaaatta agaaaaaaca ttgcatttta atttagataa
    22381 cggtatagta attaacaacg aaagcataaa acttaataga attcctttaa gtttagtccc
    22441 gtgaggctaa gaagggtggg agcaaggata cgtatggtgc gccataagta gattgctcat
    22501 tgacatagta tagctatcag tgtatcagta tacgctcagt ctaaacattc ctcctgtcct
    22561 cttagggcag gagttttttt atgcaaaaaa gtagggggaa tgaaaatgca agcaaaagaa
    22621 gtagtagatc aggtaaccat gaagcgagca ttgactcgaa ttacttatga gattatcgag
    22681 agaaaccata gtatccaaga tatcgtattg gtaggtatca aaacacgagg aatctacatt
    22741 gcatcaagaa ttgccgaacg cctgaagcag ctagaagata tcgatattcc tgtaggagaa
    22801 ctggatatca ctttataccg agatgacaaa aaagaaaatc cagaagaacc ggaacttcat
    22861 tcttctgaca tcccagtttc cttagaagga aaagaagtta ttttgattga tgatgttttg
    22921 tatacaggac gaacgattcg agcagcaatg gacgcagtaa tggattttgg cagaccaaga
    22981 aaaatttcat tagcagtcct ggtcgatcgt gggcatcgag aattgccaat ccgtgcagat
    23041 tatgttggga agaacattcc aacatctcgg gcggaagaga ttttagtaga gatgcaagag
    23101 cttgatggac aagatcgcat catgattttg aaagaggaag attagaggag agagaaacat
    23161 gtcggagaaa aaaggatttc ataatgatga cgtggtgtta gatatccacg atcgcccaac
    23221 cccaggacat tgggtaggat taagtttaca acacttgttc acgatgttcg gagcaaccgt
    23281 gttagtcccc atcttagtag gtatcgatcc aggtattgcg ctagtcagtt caggactagg
    23341 tacattggtg tacttattta ctactaaagg aaagatacct gcgtacttag gaagcagctt
    23401 cgctttcatt gcagcgatgc agcttttgat gaaaacagat ggttatccag cgattgcaca
    23461 aggagcagtc acaactggat tagtctattt gattgttgcg ctgatcatca aaaagatcgg
    23521 ttcagcatgg ctggataaaa tcttgcctcc aatcgtcgtc ggtccagtca tcatggttat
    23581 cgggttggga cttgcggcaa atgcagcaac aaatgctatg tataatcagg gcacgtatga
    23641 tttcaagtat attgctgtag cgctgatcac attaggatta acaattttct tcaacatgtg
    23701 gctaagaggt tttctcggat tgattccgat tttactaggt attattagcg gatacatcgt
    23761 agccttgctg tttggaatcg tggatactaa gccaatcgta gacgctgcct ggtttgctgt
    23821 tcctaacttt gagattccct ttgtacaata tgaaccaaag ctatatcttg gagcaatcac
    23881 aacaatggca cccatcgctt ttgtgacgat gacagagcat atcggccatc ttatggtatt
    23941 gaataaacta actaaacgta atttctttga agaaccgggt cttcataaga cgttgacagg
    24001 tgatggatta gcacaaatcg tggcagcctt tgtcggaggt cctcctgtaa caagttacgg
    24061 tgaaaacatt ggtgttctgg ctatcacacg tgtccatagc gtgtttgtga tcggtggagc
    24121 tgcagtattt gcagtcattt taggattcgt aggaaaatta agcgctttga ttttaagtat
    24181 ccctggacca gtcatctcag gaatcagttt tatcttattt ggagtaattg ccgcaagtgg
    24241 aatgaagatt ttaatcgaga acaaaatcga ttttgataaa aagaaaaatc tgttgattgc
    24301 ttcagttatc ttagtagtag gaattggcgg actcgtcttt gaagtaggca catttacatt
    24361 gtcggcaatg gcattagcaa ctgtacttgg catcatttta aatcttatct taccagaaac
    24421 ttcacgaagc gaagaaaact aaggaggaaa cattatgatc atcaaatcag aacgtatcag
    24481 cttaaagcat ttattgaccg ttgaggcact tactgatcaa gaagttatgg gcttgatccg
    24541 cagagggcag gaattcaagc aaggtgctaa ttggactccg caaaagcgcc agtactttgc
    24601 tacaaacctc tttttcgaaa atagtactcg cacacataaa agcttcgatg tagcggagaa
    24661 aaaactaggg ctagaagtga ttgaatttga agcaagcaca agctctgtcc aaaaagggga
    24721 aacactatac gatacagtac tgacgatgtc agcattaggc gtagacgtag cggttatccg
    24781 tcatggtgat gagaactatt atgacgaact gatacaaagt aaaacaatcc agtgttcgat
    24841 tatcaatgga ggagacggaa gcggacagca tccaacccaa tgcttgcttg atttgatgac
    24901 catttatgaa gaatttggtc attttgaagg tctgaaagtt gccatcgtag gtgatatcac
    24961 tcactctcgc gtagctaaat ccaatatgca gatgttaaaa cgtctaggcg cacaagtatt
    25021 cttctcaggt cctagagaat ggtacgacga tgaattcgaa gtgtacggac attatatgcc
    25081 attggatgaa ctactagatc aagttgatgt catgatgatg cttcgggtgc aacacgaacg
    25141 ccatgatgga aaagaaagtt tctcaaaaga aggctaccat caggaacatg ggttaacagt
    25201 tgagcgtgcg aagaagttgc aaaaacatgc aatcattatg cacccagcac cagttaaccg
    25261 tgatgtagaa ttagctgatt cattagtgga aggattacag tcgcggatcg ttcagcaaat
    25321 gagcaatgga gtattcgtca gaatggcaat cttagaagca gtgctaaatg gaaaagcgta
    25381 attataggag gatgaaaaaa tgaaaacact cattaaaaac ggtcagatca atacacgaaa
    25441 aaatatgacg acaccagcag aaatttggat agaagatggg cgaatcaaag caatcggtac
    25501 aggattctca gaagccgaat ttgatgaagt gtttgatgca aagggacaat tgatcacacc
    25561 tggtttagtg gacgtccatg ttcatttgag agagccagga tttacataca aggagacgat
    25621 cgaagctggg actcgctcag ctgctcgtgg cggtttcact actgtatgcg cgatgccgaa
    25681 tttaaatcct gtacctgata cggcagaaaa gctgagacaa gtatatgaca tcattagaaa
    25741 agatgcagtt gtgaaggtat tgcagtatgc gccaatcaca gaaaatctgc gaagtgagaa
    25801 attagttgat caagaagcac tgattgaaga aggtgctttt gctttcacaa atgacggtgt
    25861 aggtgtccaa acagctggga cgatgtatct ggctatgaaa gaagctgcaa aaaacaataa
    25921 agccctagta gcccatacag aagatgaatc tttattattt ggcggcgtta tgcacgctgg
    25981 caagaaggca gaagaacttg gtcttcccgg gatcttaagc gtaaccgagt cttctcaaat
    26041 tgctagagat ctgttgctag ctgaagcaac tggtgttcat taccatgttt gccatgtttc
    26101 tactaaagaa agtgtccgtg taatcagaga cgcaaaaaaa gcaggaatcc acgtaacagc
    26161 agaagtttca ccccatcatc ttattttgat cgacgaagat attcccgaag atttcggctt
    26221 ttggaaaatg aacccgccgc taagaggacg ggaggacaga gaagctttga tcgaaggctt
    26281 gcttgacggt acgatcgatt gcatcgctac agaccatgcc ccccatggat tagaagaaaa
    26341 aagccaaagt ttcatgaaat caccatttgg tatcgtagga agcgaaacag cgtttcaact
    26401 gatctatact cattttgtag aaactggtcg attcacgttg gaacaagtga tcaattggtt
    26461 ggcagttaaa ccggctgaga tttttggttt gaatgcagga acgctaacgg ttggtgcccc
    26521 cgctgatgta gcagtttttg atattacaca aacttgcacg atcgataaag aagacttttt
    26581 atcaaaaggg gagaatacac cttttatcgg ctggaaagta aaaggcgaaa cacaaatgac
    26641 ttttgtcaac ggaaaacttg tgtggcagaa gggagaataa aagtatggaa cgtttgctta
    26701 tattagaaga tggaacagtt ttcgaaggaa aggcttttgg tgcagatgct agtgtcttcg
    26761 gagaaatcgt ttttacaaca agtatgactg gttatcaaga aactatcact gatcaaagtt
    26821 ttaacggtca aatcattaca tttacctatc ctatggtggg taattatggg gtaaatagag
    26881 atgattatga atcgatttct ccaacatgca aaggagtagt agtaaaagaa catgcacgtg
    26941 tagcttcaaa ctggcgaaac caaatgacgc tagattcatt tttgaaacaa aaaggaattc
    27001 ctggaatatc aggtatcgat acgcgcgccc ttactcgcaa aatccgtgaa gtcggaacga
    27061 tgaaagctag tatagttgat gctggagatt ccttcgaaca tgcatttgat cagctgaaag
    27121 caaccgttct agcaaccaat caagtgcagc aagtctcaac taacaaacct tatccaagtc
    27181 caggaacagg tcggaaagtc attgtcattg acttcggctt gaaacatagt attttacgag
    27241 aattgagtaa aagaaactgt aacctcacag tacttcccta taacacagat gccgagacga
    27301 ttttagcgct atcaccagac ggagtcatgc tgacgaatgg acctggtgat ccaaaagatg
    27361 tgccagaagc aatcgaaatg atcaaaaaaa tccaaggaaa agtaccgatt tttggtattt
    27421 gtctcggtca ccagttattt gctttagcca acggtgcaga tacctataaa atgaaattcg
    27481 gacatcgagg attaaatcac ccggtacgag aagtagcgac cggaagaatt gattttactt
    27541 ctcaaaatca tggctatgca gtcgatgaag ccacgattga tccagaaaaa ctcatcgtaa
    27601 ctcatgtaga agtaaatgac ggaacagtag aaggagtcaa acacagagat tatcccgctt
    27661 tcagtgtgca gtttcatcca gatgctgccc ctggtccgca tgatgcacta catttatttg
    27721 atgaatttat ggaaatgatg gacgcaggaa aggagcagta aacagatgcc gaaacgtaca
    27781 gatatcaaga aaattatggt gattggatct ggtccgatca tcattggtca agccgcagaa
    27841 tttgactacg caggaacaca agcctgcctt gccttaaaag aagaaggata tgaagtggtt
    27901 ttagtaaatt ctaatcctgc gacgatcatg acggataaag aagtggcaga tcaagtatat
    27961 atcgaaccga tcacacttga atttgtatca aggattcttc gaaaagagcg tccagatgct
    28021 ctccttccaa ctttaggcgg acagacaggc ctcaatatgg caatggagct ggcagcatca
    28081 ggtattttag aagaattgaa catagaactt ctaggaacaa agctttctgc gatcgatcaa
    28141 gcagaagatc gtgatctatt caaacagttg atgaaagaat tgaatcagcc aatccccgag
    28201 tctgaaatcg tcaatacagt agaagaagct gtagattttg caaataaaat cggctatcct
    28261 gtcatcgttc gtccagcctt tactttaggc ggaactggtg gaggtatgtg tgaaaatgaa
    28321 gaagaattac gtatcgtatc agaaaatgga ttaagtcttt cccccgctac ccaatgtttg
    28381 attgaacgaa gtattgcagg ttttaaagaa attgaatatg aagtgatgag agattcggct
    28441 gataatgcaa tcgttgtctg caatatggaa aattttgacc cggtgggtat ccatacaggt
    28501 gattctatcg tatttgcccc tagtcaaaca ttgtctgacc atgaatatca aatgcttcgt
    28561 gatgcttctc tttcgattat tcgagcactt aagatcgagg gaggctgtaa tgtccaactc
    28621 gctcttgatc cacatagttt taattattat gtgattgaag tcaatccgcg tgtttctcgt
    28681 tcatctgcgt tagcaagtaa ggcgactggc tatccgatcg ctaagctagc tgctaaaata
    28741 gcgcttggat taacattaga cgaaatgaaa aatcctgtta caggaacgac ttttgcagaa
    28801 tttgaacctg cattagatta tgttgtagct aaaattcctc gctggccgtt tgataagttt
    28861 gaaaacggag aacggctttt aggcacgcag atgaaagcaa ctggtgaagt gatggctatc
    28921 ggtcggaata tcgaagagtc tttattgaaa gctgtgcgtt ctttagaaat cggtacacac
    28981 catatcgaac ttcctgagtt ggcagacatc acggacgatg acctaatcca aaaagtggtt
    29041 cgagcacaag atgatcgcct gttttatgta gctgaagcta ttagaagagg gtacaccata
    29101 gaagaattag cggatttgac aaaaatcgat ttattctttt tagataaact gttgcatatc
    29161 gtagaaatcg aacagcagtt gaaacagcag ccgttggatg acaagatatt gaaaacagcg
    29221 aaacaaaacg gttttacaga caaaaaagtg gcggagctgt gggaaataaa cgaagaagcc
    29281 gttcgccaga tgagaaaaga aaatgatttg attcctgtat ataagatggt tgacacttgt
    29341 gcagcggaat tcgaatcaaa aacaccttat ttttatagtt cctatgaata tgaaaatgaa
    29401 agttcacgat cagaaaagcc ctcaattcta gtactgggat ctggtcctat ccgtatcgga
    29461 caaggggttg aatttgacta tgcgactgtg cattctgtca aagcaatcca gcaggcagga
    29521 tacgaagcaa tcatcatgaa cagcaatcct gaaacagttt cgactgactt ttctatttcg
    29581 gataaattat attttgagcc attgaccttc gaagatgtga tgaatgtgat cgagctagaa
    29641 cagccgatag gagtaatcgt tcaatttggt ggtcagacag caatcaactt agccgaacct
    29701 ttgactgctg ctggtgtaaa aatcttaggg acaacgatcg aagacttgga tcgtgcggaa
    29761 aatcgtgatt tattcgagca agcgctgaaa gagttggata ttcctcaacc tttaggggat
    29821 actgctacaa gtaaagaaga agccgtggtt atcgcttcga gaattggtta tccagttctt
    29881 gttcgtccaa gctatgtatt aggcggacgt gcgatggaaa tcgttgaaaa ccagcgagat
    29941 ttagaagatt acatggaaca tgctgtgaaa gcatctccag aacatccagt actagtggac
    30001 cgttacttgc ttggtggtga atgcgaagtg gatgcgattt gtgacggtga aacagtactc
    30061 atcccaggga tcatggagca tattgagcgt gctggtgtcc attcagggga ttcaatggct
    30121 gtgtatccac ctcaaacatt atctgaatcg atcaagaaaa cgatcgaaga ttacacgatc
    30181 cgcttagcat taggtttgaa ttgtgtcggg atgatgaata tccagtttgt catccatgaa
    30241 aatgaggttt atgtgatcga agtcaatcct cgagcaagtc ggactgttcc attcttaagt
    30301 aaggtgacag gaattccaat ggcacaaatt gcgacgaaag ctattttggg agagaaacta
    30361 aagacacttg gttatgaaaa cggactttat ccagaaacaa aagaagttca tgttaaagca
    30421 cctgtctttt cttttacgaa attgcagaaa gttgatactt acttagggcc tgaaatgaaa
    30481 tcgactggag aagtaatggg atctgacaga caattagaca aagctttgta caaggcattc
    30541 gaagcttctg gattacactt gcctgaatat ggagccgtat tatttacgat tgcggacgaa
    30601 acaaaagaag aagcactaga attagctaaa cgttttacag aaattggcta tagtctgata
    30661 ggaacgaaac atacagctgc ttatttcgaa aaaaatggtc tggtcgttga aacagtagcg
    30721 aaaatttctg aggaagcagc agaaaaaaat gtagtcgact taataagaga tggtaaaaca
    30781 caagtggttg tcaatacgat tgataaagat cggggaaatg cttctaaaga cggtttcatc
    30841 atccgaagag aagcagttga acatggtatt ccattgttta cttctttaga cacagcagat
    30901 gcgatcgttc gagtgatgga atcccgtgct ttttcaacta accctattta acggcgagca
    30961 catgttaaga caaaaagacg aaaaaccgtt gcaggagggg acagatgaaa caagaaatga
    31021 tggaaatcgt tcagcaaaaa cagctggcac caaagatttt tgaaatgaca ttgaaaggtg
    31081 atctagtaaa agaaatgcag caaccaggac agttcttgca tattctcgtg ccgcgtgcag
    31141 acttgctatt acgccgaccg attagcttga atcatatcga ccacgaaaca aatacttgcc
    31201 gtatcattta tcgggtggaa ggtgagggga ctagagtctt ctctactctt tctgccggag
    31261 accatttaga tgtcatgggt cctttaggca atggttttga tttgtccaat cttcatacag
    31321 gagacgaagt atttataatc ggtggaggaa tcggtgtccc tcctctatat gagttatcaa
    31381 aacagttagt cgaaaaagga attaagccaa cgcatttttt aggttttgcc acacatgaag
    31441 tcgtttatta tgaaaaagaa tttaatcgtc taggagaaac aagaatcgct actgacgatg
    31501 gaacatacgg tatgcacgga aatgtcggta atttattgtt aggaacgaag acggaaccta
    31561 cagctgtttt tgcatgtggt tcaaacgggt tattgaagac agtggaacag ctgttttctt
    31621 cgcatcaaaa tgtccagtta tcattagaat cccgcatggc atgtgggata ggggcttgct
    31681 atgcttgcgt ttgtcatacg ccggaagatg aaacaggaac aaaaagtgtg aaagtttgtg
    31741 acgaaggacc gatttttaaa ataggagagg taattatatg aatgagaatc ctttagcggt
    31801 caaacttcct ggcctagaat taaaaaatcc tatcatgccg gcaagcggct gtttcggttt
    31861 tggcaaagaa tacggaaaat actatgattt gaatcagctt ggatcgatca tggtcaaggc
    31921 aacgactcca aatgcccgct ttggcaatcc cacgccaaga gtggcggaaa caccatctgg
    31981 catgttgaat gcaattggtt tgcaaaatcc tggtctagac gttgttatgc ataaacttct
    32041 tccagaatta gaggagtaca atgagttacc catcatcgca aatgttgctg gtgcttgtga
    32101 agaagactat gtagaagttt gtagcaaaat tggcgaagca ccaaatgtga aagcaatcga
    32161 gctgaacatt tcttgcccaa atgtcaaaca tggcggaatt gcttttggaa cagattctga
    32221 tgttgctttt caattaacac aagcagtaaa gaaagtttct tctgttcctg tctatgtcaa
    32281 attgtcccca aatgtaacag acatcgttcc aatcgctcag gcaatcgaag ctggaggggc
    32341 agatgggttt acgatgatca atactcttct tggtatgcgg attgacctta agacaagaaa
    32401 accgatttta gccaatcaaa caggtggact ctccggacca gcaatcaagc ctgtcgctat
    32461 tcgtttgatt caccaagtag caagtatatc cagccttcca atcattggga tgggcggcgt
    32521 gcagacagtg gatgatgttc ttgagatgtt tatggctgga gcaagtgctg tagctatagg
    32581 gaccgcgaat ttcaccgatc cgtatatctg tcctaaacta attaaagagc tccctgtgcg
    32641 aatgagcgaa ctaggcatcg aatcgctgga aagattaatc aaagaagtga gagaggaacg
    32701 tgaacgatga atcaactaga ccaacgacca attattgcat tagatttttc aactaggcaa
    32761 gaggtagaag actttcttcg atttttcccg aaagaagaaa aattgtttgt gaaaatcgga
    32821 atggaattgt tctaccaaga aggacctgaa atcgtacgtt atctaaaaga ggctggtcat
    32881 aatgtatttt tagatctgaa acttcacgat attccgaata cggtcgaaaa agcaatgcgg
    32941 ggattggcaa agcttggtgt tgatctcacc tgtgttcatg ccgctggcgg tattcgtatg
    33001 atggaggcgg caatgcgagg attagaagag gggactccag aaggtggaaa acgtccgcta
    33061 cttctagcta tcactcagct tacttctaca agtgaagaag aaatgcatgc cgatcaacta
    33121 atcgaagttt ctttagaaaa aagtgtgatc cattatgcta gctgtgccaa aaaagcagga
    33181 ttagatggtg tggtttcttc tgcttgggaa gtagaagcga tcaaagaaat ggcaggagac
    33241 gaatttgttt gcttaactcc gggtataaga ccagaaggaa cagtcgcagg agatcagaca
    33301 agagtagtca caccttctca ggcaaaaaag atcggctcaa cttttatcgt tataggtcga
    33361 ccaatcactc agtcaactaa tccttataaa gcatatcaaa cgattcaaac agaatggagc
    33421 taacctaaaa tgaatgttga acaaagtatc gcaaaagact tattagaaat tgaagcagta
    33481 tttttaaatc caagtaatcc ttttacatgg gcaagtggaa tcaaaagccc aatttactgt
    33541 gataaccgaa tcacgatgag ctatccaaaa gtccgaaaag agatcgcaaa aggattagca
    33601 agcaaaatca aagaagctta ccctgaagta caagtgattg ctggaactgc aacagcagga
    33661 atccctcatg ccgcatgggt agcggaaata ctggatttgc caatggtcta tattcgaagt
    33721 aaggcgaaag atcatgggaa aggtaaccaa atcgaaggac gtatatttga aggtcagaag
    33781 atggttgtga tcgaggattt gatttctaca ggcggaagtg tcttggaagc agctgaagca
    33841 gcaaaaagag aaggtgcgga tatccttggt gttgcggcta tctttacgta tgagttgcca
    33901 aaaggaaaag caaatttcga aaaagccgaa atccctctta tgacgttgac caattattct
    33961 gtattgattg aagcagcttt agaagatcgt tatatcgatg agcaagaatt aactctgcta
    34021 aaagaatgga aaaaagatcc tgaaaattgg cagatttaag cgcgcttgat gaagatagag
    34081 aaatccgatt tgtgtctcca gaatcatcaa ttggaatagc aaaaatccaa aaactgattg
    34141 cgagaagcat cttttcatca ggtatgaaaa gaaacttcta tcgtgatcag tttttgattt
    34201 ttttaaaatt catttttcta agactggtta gcagaaagaa gagaataatc tcttctcttt
    34261 tttctattat atatagattt tctctatttt ttggtaaggg agctgtttca aaaatggtaa
    34321 agagcggaaa tgttgcgaat atcagttatt taatgatatg atattcggaa aaagaaatct
    34381 caaagggagg taacttttat ggggttgaat gaatttattt taggattgaa caatttagag
    34441 acgatcacaa gggcacctgg attttttaaa tatacagagc atacagttgc agcacattcg
    34501 tatcgtgtag catctattgc gcaagttcta ggagacattg aagaagcgaa tggacaaccg
    34561 atcgattgga aactgctcta tgaaaaatca ttgaatcatg actacacaga gcgttttatt
    34621 ggtgatatca aaacgccggt gaaatacgca agcaaagagt taagagggat gctgcaaaca
    34681 gttgaagaaa agatgacgga cgaattcatc aaccaagaga ttccagctga gttccaagaa
    34741 gtttatcgcc gtcggttgtt cgaaggaaaa gatgaaaccg tagaaggaga actattagcc
    34801 atttcagata aggttgacct tctttatgaa tcttttgatg aaatcatcaa aaataacccg
    34861 gaaaaagttt ataaggagat gttcattgag gcagtgacta cgattaaaag ctttgaacat
    34921 cggccttctg ttcagtattt ctttaaagag atatttccgg aactattgaa ccaggaattt
    34981 tatggaaaag aggcgttttt agaaaccgtc tcaacgattg taaagaaaga agaaaaataa
    35041 aaatgatatg agatgcaggg ggaagtatag ttgaaaaaga atatggatgt cgaatggggc
    35101 tattctggag acaaaggtcc ggaaaattgg gcgagtttat gtgattggtt tgcgagaggt
    35161 gcggaatttc cattgcagtc gcctattcat ttgaccaagg gggaagaaac caaaatagaa
    35221 ggagacacgc tttcctttca ttatcaaaag caacggttta ctgacaagga attcaaaaac
    35281 acgattcatt tagtaccttt tgaccaactc agttatgtag agttcaagaa agaacgctat
    35341 tatttgacag atattcattt ccacttgcct tctgaacata tcatcaacgg aaaacaggaa
    35401 gatattgaat ttcattttgt tcatatgaac agcaaaaaag agaatctagt tgtgggtgtc
    35461 atgtaccagc taatggtaca ggaaggttgg ctgtataatc aagaaaatgg ggaaagttgg
    35521 aacttagaaa aacatgagca ttgggtaaat ccggatgtgt tctttccgga gcaaaaaagc
    35581 catttccatt atattggctc attgacaact ccgcctacca gcgggccaat ccaatggttt
    35641 gtaatggatc atgtaggaaa actgaaccaa gagtttcttc tccccttcga tggtcagtat
    35701 gctcgaccga acaaccgacc aatccagccc ttgaatggaa gagaaattta ttatttcgag
    35761 gagtttgagc aatgaatatt cagcagatga aatacgtggt cgctgtagca aacaatggta
    35821 gtttcagaga agcggcaaag aaaatgtttg tctcccagcc aagtctttca gctggtatca
    35881 aagaattaga aaccgagtta gggatcacgc tgtttactcg aacgaatcga ggggcatatt
    35941 taacagaaga agggcaagac ttcttaaatc gtgccgagaa aatattggtc cagcttgata
    36001 cattagaaac tcactatcta acgagtgaaa agagagacaa tttttcgatc gctgcccagc
    36061 attatgattt cttaggtcct ttgaccgcac agctactcga ggaattcaaa gacgagatca
    36121 aacaatttcg gatcatggag acgacaacag cgagagtgat cgaagaagtc aaagagcagc
    36181 acagtgagat cggcattatt tatatgaatg agcataatca agctggtatc agaagatatt
    36241 tacaacaagg agagctaagt ggccgcagca ttggaaaatt caaaacgcat atttttttaa
    36301 ggcaaggaca tccattagct gaacacgcag agatcaccaa agaagaattg ttatcttatc
    36361 ctcaagttcg ttttacacag gatggaaata atttcccgta tttttatgaa gaccttatcg
    36421 agatccctga tcaagaaaca gtgatttata cgagtgatcg aggtactttg atgaatatcg
    36481 ttcttgaaac tgatgcttac gcttcaggat ctggtattgt gattggaggg attcgcgagc
    36541 acttgcggtt aatccctttg gcagaaggcg aactaaatga attttatatc atttattcgg
    36601 cgaaacgtcc gctcagtgaa atcgctcaaa gatttatcaa agagcttact cggttactgg
    36661 accagcagag ctaagaaaag cagatgagaa cgctgtttat tgaacaccgt tgatcaaaaa
    36721 agaggggttg tgacagaatt ttgtcacaac tcctcttttt gtagcaaaca atttataact
    36781 aattgctgtt gtgggaaagt acgcaaatat gattctagaa acactcattg attacctgtt
    36841 ttgtttcata gctgtaattt tttcttcttc tggtgtcacg ataatcgtct tttggttttc
    36901 ataaataaca taaccaggct tggcgccatt tggttttcgg atatgcttta cctgtacaag
    36961 atcaaccggc acttgggcag agtatcggta ttttgaaaaa taggcagcta actcggcagc
    37021 ttctgtaatt gtttcatctg agggcttgtc acttttaatg atgacgtgcg aaccgggtat
    37081 attttttgca tgaagccaat agtcggtttt tttagctgtc ttcattgtca gctggtcatt
    37141 ttgaagattg tttttaccaa caagaatgag cgttccgtca gaagaaaagt attgatcagg
    37201 ctgagatggt ttcttacgtt tttgtttttt taagttcttc tttttcaggt atccttctga
    37261 agtcaattct tctttaattg cttcgatatc cattgggccg gcaatttcca gttgagataa
    37321 gacagattca agatattgga tttcatcttt ggcttcttgg atttgttcgc caatcagctt
    37381 aacagcgttc ttcagctttt ggtatcgatg gaaatatttc tgtgcatttt gatttggtgt
    37441 caatgccgga tctagtgcta ttttgattgg ccgatcttct tcataataat taggtaaaac
    37501 aacttctttt gcgccgcgtg gtacttgtgt cagaaaagta gtaagtagtt cgccatttct
    37561 acggtaattc tcagcgtttt cggattcttt gagtgtttgt tcacgttttt tcaatttatt
    37621 tttatttcgt tttagttcat tttcgatttt tcgaatcagt tctccgcctt gttgttttgc
    37681 ccgatctttc tctgcttttt cgtgataata gctatctaat aattgactga gtgttggata
    37741 agctgttaca gcagatgcct gttcggacaa tacctgataa gcaatcggtg tgaaaaaatc
    37801 tttattttcc gtttcgtaaa aagtaggaat aggctgatgg ctgatggctg agaaaaattc
    37861 gtcccataca accatctttt cattaggacg ttccagtaat cttttggtca gctcttgagc
    37921 gatatcgaaa ccgattccct gaaactgacg ttggattgct tttggatcta gttcagtttg
    37981 ggaaagccgc tgaaagattt tttctttctc ggagctaaat gggttcaaga cctcttgttt
    38041 tggcggagca acatattcta caccaggaag caaagaacga taagtgtttt gagagcttcc
    38101 aatgtgtttg atcgcatcca gtatttttcc agtttctcga ttaactaaga cgatcgtact
    38161 atgacgtccc attaattcaa cgatcaatat gatattttgt agatcaccca actcgtttcg
    38221 tttagcaaag tggaaatgga tcactcgatc attttcgatt tgttcgatgt cttctaaaat
    38281 agcgccatcc aaatattttc gaagcatcat cacgaagtta ggcggattat caggattctg
    38341 ataatcgatt tgggtaatct gtacacgagc ataacttggg tgcgcagata ataataatcg
    38401 atggtttttg ccgcgtgaac gaatcaccag cacgatttca ttttcataag gctgatggat
    38461 tttcgagatc cggccagata gtaacgtttc acgcaattcg ttgaccatcg cgtgggtaaa
    38521 tacaccatca aaggacatgg ttcattcctc atttcttttc ataagtatat tcattataac
    38581 gaaaaaaaga agaaaaacca atttggccga atagtaaagc aaaagtctag aaaaatcttg
    38641 gactaattac ataaaaaaca gtgtttctat cgttgaaatc caaattttta ggtataataa
    38701 aaaataggag aaaaagtaga aaagaaggtg tttgttattt gggaagtagt taaatcgaga
    38761 gtacaagaaa tgaatgaaca tttctcagtg tcattcgcag aaaatccaag taagtatttt
    38821 cgacgtcttg tttttctgct catcttatca ttgctgttaa cagttgaaat gtttttattg
    38881 ccggaaaaaa tccgagaaga gcaagtaaat agtctcgttt attcgtccat accaaccagc
    38941 aaattgaaac cttttgatta cagcaaagct gatcagaaaa tcaaacaaag ccgggcgata
    39001 tcggtgatgt tcagcaaacc gaacgggaaa acctatcagg acatgcttga tgtttttaat
    39061 gatcctgagc ttatggaaga attgaatcga ccgatctttt attatccgat cgtttatgat
    39121 gtacatgaac tggaacaaaa atataacata cgaacggatg aagtgacatt tattttcttt
    39181 gatggaggaa aagaagcaaa tcggataact gcgggtaagg gaggaataac tgattttgat
    39241 aaggagttga tccctgaact gaatcgactt ccgttagcca atatcaaaca attagaagaa
    39301 gaattggata gaacgggagt aacttcccaa aattccgaga ttgctaattg agacattggc
    39361 gcgatgaaac ggtgggtcta tatagcgcta tttttaatta tcagaacaag tgatgtttga
    39421 tcttcaaaat aacaaataaa aaattaaaaa atatagttga cgaaattcaa aaattcgatt
    39481 agacttaaat caattcttaa ggaaagggtg acctgtaatg aacttatcaa aacgtatttc
    39541 agcagaaaac aaaaaaaatt ggcgcagctg gcgtagataa gttgtattaa tggtgagact
    39601 ttcccataga tacaactttt tagagtgcat ttctaaagct tgtatctatg ctagacatga
    39661 acattgactg ccgcatagat tagaatctat gcggtttttt gtgtaaagac aatgaccgct
    39721 ttggccattg tctttttttg tagactggat acttgcctga ttataataaa taaaaagatt
    39781 gactttgcag gaggaaaaaa atgaaaaaca aacgattaat aacagttgta gtaattattt
    39841 tagtttattt agcaggtgca tttatttatg gaaaagcctc aggcgaaaaa gaaagtactt
    39901 cgacgaacca gacaaaaaaa acaacagttg ttggggtatt gcaatacgtg agccatccgg
    39961 cattagatga gatttatcga ggaatcaaag atggtctgaa agaagaagga ttaaaagaag
    40021 gagaaaatct tgagatcaaa tttcaaaatg gacaagccga tcagagcaag ctagccacaa
    40081 tgagtcaaca gctagtacaa tctgatccag atgccttgat tggaatcgca acacctgccg
    40141 ctcaatcatt agcaaacacg acttcatcga tcccagttgt gttaggtgct gtaacggatc
    40201 ctgtgggggc tggattagtc gcttctttag agaaacctgg tggtaatgta acaggtgttt
    40261 ctgatcagcc gcctgttact tcacaaatca agcttgcttc tgatttgttg ccaaatgcaa
    40321 aaaaagtcgg aatcttgtat gcttcttcag aggataattc aaaataccaa gtagcacaag
    40381 cagaagaagc tgcaaaaaaa gaagggttag aatcagtaaa atatgcggtg ccttccacta
    40441 atgagattgc acaaacggtt caagtgatga gccgacaagt cgattttatc tatgttccat
    40501 tagataatac cattgccaat gcgatgcaaa cagtggtaaa agaagcaaac aaagcaaata
    40561 tccccgtcat tccatctgtg gatacaatgg tggaacaagg tggactagca acaatcggga
    40621 tcaatcaata tcaattaggg cttcaaagcg gtaagatggc ggcagaactt gcgagtggaa
    40681 aagaaaaacc ggaaacgacg cctgtttata catttgatca aggggataca gtgatcaatc
    40741 aatctcaagc tgatcgctta ggaatcattg ttccccaatc gatgaaggaa aaagccaaaa
    40801 tcattacaga tgactcgcag caagaaacat ctaaggagga tgacaaatga tcgtatcagc
    40861 tatttcacaa ggcatgcttt gggcaatatt aggattaggg attttcatga cttatcggat
    40921 cttagacttt cctgatatga cgacagaagg gtccttccca ttaggtgctg ctgtctgtgt
    40981 gacagctatt actcatgggg tagcgccgat tgtagctaca ttattaggcg taggtgctgg
    41041 catgatagca ggactcgtta cggggttact ttatacaaaa ggaaagatac cggttatttt
    41101 agcagggatt ttagtgatgt caggattgaa ctcagttatt ttgtttgtga tgcgaacacc
    41161 aaatctttct ttattgaatc atccgattct acaagacagc ttccatcgct ttgggttacc
    41221 tgattacttt gacacagtcc ttcttggctt catctctttg ggcatcttga ttgctgtact
    41281 gctattcttc ttcaatacag atttgggtca aggctatatt gcgacaggag acaacgaaac
    41341 gatggctcgg tcgttaggga taaaaaccga tcgtatgaaa attttaggtt taactttgtc
    41401 gaacggtgtc attgcattat ccggtgcgct gattgcccag aacgacggat atgcagatgt
    41461 aaataaaggg attggcgtaa tcgtcatcgg tcttgcttcc atcatcatcg gtgaagtgat
    41521 cttccaagaa ttgacgctag cagaaagatt gattgctatt gttgtcggca gtatcattta
    41581 tcagctattg attttagtag taatcaaact agggtttgac acgacttatc tgaagttgtt
    41641 ctccgctatc atcttagcta tttgtctgat gatcccgcaa ttgaagaaag cactgaactt
    41701 aaaaacgggt tttgagaagg aggcatagta atgactgata tattgacaat caaaaatggc
    41761 agaaaagtcg taaacaatgg aatgaatgaa gagaaagtac tattggatga aattaacctt
    41821 acaatcagag aaggggactt catcactgtc cttggcggga atggtgctgg aaaaagcaca
    41881 ctgttcaata cgatcgctgg tacgcttcaa ctcagtcaag gatcgatctc ttttaaagat
    41941 cagctgatca caaaagattc agaagaaaaa agagcacgat tcctttctcg agttttccaa
    42001 gaccccaaga tgggaacggc tcctcggatg acagtagcag aaaatttgct acttgcacaa
    42061 aaaagaggtc aaaaacggaa attccgttta cgtaacttga aaaatcaaaa agaagatttt
    42121 ttcagactat gtcaagaagt cggtaatggc ctagagcaac acttggatac tccagcgggt
    42181 gaattatctg gtggtcaacg ccaagcatta agcttgctga tggcgacgat cacagctcct
    42241 gagttactat tattagatga acatactgca gctttagatc ctaaaacagc taaagcatta
    42301 atgcgattaa caaatcagcg gatcaatgaa caaaaattga cttgtctcat gatcacgcat
    42361 cgaatggaag acgccttgaa atacggaaat cgtctgatcg tactccaaaa agggaaaatc
    42421 gttcaagata tttctgaaaa agaaaagcaa catttgacaa tgggcgacct tttgcagttc
    42481 tttgaagaag tagtggattg atttcgacag aaaaattcct ttcagtagaa ggacttgaaa
    42541 actcattgac atcttccttt gattcatcct atacttaaaa caataaacaa ttaaacatat
    42601 ttgggtgttt tgtaggatca cgtagtcaat gattttaaca attaaataga atattctttg
    42661 ctctgacaag taaacgatga gaaaagaatt tgctactttg ccacctagac gtttcgtact
    42721 ttttcgtatc aaacgctaag tggctttttt tattgaagta agaacaaagg aggaataaga
    42781 tgatttctgg acaaaccaaa ttagctgggc tctttgctga tccggcttct cacagtttat
    42841 ccccattgat gcacaatact gcttttgaag caaacaacat cgatgcggtg tatttagctt
    42901 tccaagtaaa tccaatgaat ttgaagcaag cagtcgaaag tattcgaact tttgatatgt
    42961 taggtgtaaa tctttcgatg ccgaataaaa cagcggttat tccttatctg gatgaggtaa
    43021 gccaggaagc gcaattgatc ggagcagtga atacgatcgt gcacgaaaag ggtcgtttga
    43081 ttggctataa tactgatggt gcgggcttca tgcaatccgt gcaagaagct gggatttcga
    43141 tcaaaaagaa aaaaatgact ctactgggag ctggaggtgc agcaaaggca gtcactgttc
    43201 aagcagcgtt agacggcgct gaggaaatcg tagtttataa acgaaacaac caaacattcg
    43261 ctcaagtaaa agaaacattc gaacaactca gctatcgaac ttcttgtaag ataaaagttc
    43321 gtgactttgc cgatacgcat caattaagaa aagacttgaa agaaagctgt cttctagtca
    43381 atgcaacaga tatcgggatg ggaaagaaaa tcaatcagtc accgctctta gatataagca
    43441 tgatgcatga gaatctagcg gtttttgatt tgatttatgc acctagagaa acacgtttat
    43501 taaaagaagc aaaaaaagta ggagcaaaag gatacaatgg acttggcatg ttgatctacc
    43561 aaggagcaat cgcgtttgaa ttatggacaa aggaaaagat gccgatccat gtgattgaaa
    43621 agctgtttga ttaaaaaagg ataatagata aagatctttt attctcgcat aaacggtctc
    43681 aagaaaagta atcctttatg tatcgaagca tttatttcgt tattttttat taaattatca
    43741 aaaaatgata ggcttttgtc gattggttat cgtatccttt atttatcagt aagaaacgag
    43801 gaggaaccac aatgagtcgg atggatcgct ataaggacat ccataagaag gcgaaacctc
    43861 tgaagcggga tacggggagt atttttgcta gaagagaacg aaaacgaacg cgtatggaaa
    43921 atgaggaaga tgctacccgt atttatgaaa gagagcagca ttcatttgaa catttgaacc
    43981 aatacgagca acagatatac agagatgcac caagagcaga agaacaacaa aaaaataaaa
    44041 aatttttcaa tcggcaaaag aagaacaaac cgttaaaacc tcgaagaaag cgatcgtgga
    44101 aaaaaatcat tctatcgatt ttgcttgttt tgatccttta ttcaggtatc tcatttttta
    44161 ttggtaaata tgtagctgaa catgacacaa gcttagaggc agcgacttca gaaacctttc
    44221 atgggcaaaa agcgacgaat ggtgcgcata atattttgat ccttggaagt gatacacgcg
    44281 gagaagatgc cgggcgagcc gatacgatca tggtcttaca gttggatggt ccttcccata
    44341 agccgaaact gatttctttt atgcgtgaca gcttcgttac gatcccaggg gtaggacaaa
    44401 ataagatcaa ttcagcttat gcatacggag gagccgatct agtgcgtcaa actttagtag
    44461 aaaattttgg aatcgattgt caatactatg cgaaagtcga tttcaaatcg tttgaaaaag
    44521 tgatcgatgc cttattcatg aacggagtga agatcgatgc agaaaaagat ctgaatctgg
    44581 atggtgtaga tattaaaaaa gggggccaga agatggatgg acatgttctt ttgcagtacg
    44641 cacgtttccg aatggatgaa caaggagact ttggccgtgt cagacgccaa cagcaagtaa
    44701 tgaacgctat tttcagccaa ttgaagaatc cattgaatct gatccgtgca ccttatgcag
    44761 ctggtaaagc tttaggatat acatccacag atgtcactgc ttggttcttg acgaaaaacc
    44821 tcttttcgat tgcacgagga gctggtggca ttgatcgaat aagtgtgcca gtagacggtt
    44881 cgtggaatta tgggcaaagt gattatgctg gcagtattct tgaaattgat aatcaaatga
    44941 atcaatctgc tattacagat tttttgaata aatgatcata aaaaagatca agaagaagct
    45001 gcgcaaggct tttgtcatga atcaatcaaa cgattcactc gtaaaaaacg ctttcttatg
    45061 gtagatttaa ccaagagagg agctgtttac attggatatc tatctgaact tcaaaaatga
    45121 gtcgcgtgaa gccatcgctt tttatgaaaa agtttttcag actgaagcat caggtatcac
    45181 cacatacggt gaaatgcctg gaatagaaca aatgaacttg ccagaagaag caaaaaatct
    45241 aatcacgaat gcacggctga acatttatga tacattagtt atgtttgcag atacgccaga
    45301 aggaattggt cctgaattag ttgtgggaaa taatgtttct ttagtcatcc agacaagaaa
    45361 tgaagaagaa acgaagcgcc tatttgaaag cttagctgag ggtggtaaga ttgagatgcc
    45421 actggatgct gttccgtggg ctcagttgta tgggatggta actgatcgat ttggcatcaa
    45481 ctggcagatt aaccaagagt gacagctaaa gatgaatatt ttctggaaac atcaagcagc
    45541 aaacggatca tcgtttgttt gcttgatgtt tttgtgtcta tttcgactat acggtatctt
    45601 ttttttattg ccagatgcga aaatatgttc cataatagag aagtccgcaa aaaattaagt
    45661 aacggaggtc agaagatgaa gacggtgata ctagctgaaa aaccttcaca agcaaaagcc
    45721 tatgccgaaa ccttccaaca gcaaaagcgg catgaagggt attatgaaat cagcgatcca
    45781 ttgtttgctg gtgaagtaac catcacttat ggttttggac atttggtaga tatggttgct
    45841 ccaggagctt atgaagagcg ttgggcaaaa tggtctttag aaaatttgcc tattttccca
    45901 gaaaccttcc gttacgaagt tccaatggac aaacgtgcac aattttcgat cgtaaaacga
    45961 gaattgcagt cagccgatac catcattatt gcaacagatg gtgatcgaga aggtgaagcc
    46021 atcgcgtggt cgattattcg acaagcaaac gcattttcta aggaaaagaa ctatttacgt
    46081 ttatggatca attctcttga aaaagaagca atctatgaag gttttaagaa tctccaacct
    46141 ggaaagaact attttccaaa atataaagaa gcgcaagcaa ggcagaacgc tgattggctg
    46201 attggaatga atggaagtcc attatatagc ttgctacttc aacgaaaagg gattgatggc
    46261 agtttttctc ttggtcgtgt acaaacaccg acactctata tgatttatca gctgcaagaa
    46321 gaaatcaaac acttcaaaaa agaaacgtac tttgaaggag aaggtttaat tacgacaaac
    46381 aaagggcagt tttctggaaa aatcgtacca aaaaaagcat ttaaaaccca agaagagtta
    46441 acgaaggaaa tagaaaaact tggagcgcat ttaggtaagc aaaacggacg gattcttgaa
    46501 gttacgaaaa aggaaaagag gttgcatagt ccacgtttat tttcgctatc tagtttgcag
    46561 acaaagatga atcagctaat gaaagcaagt gccaaagcta cacttgatgc tgctcaagga
    46621 ttgtacgaaa gcaagtttct aagctatcca agaacggatt ccttttacat tacagaaaat
    46681 gagcatcagt atctggttaa acatctgcaa aattataaaa aatttttagg aatagaagaa
    46741 gtacagacaa ctgagatcaa agcaaagaaa cgatatgtcg atgcgaaaaa agttcaagaa
    46801 catcatgcaa ttattccgac aaagacgatt ccgactcctg cacgctttgc gaagctttcc
    46861 aaattacagc aagcaatcta tttgcaagtt ttgcgcacta ctgtggctat gtttgctgca
    46921 gattatatct acgaagagac gaccgtaatg acgggcgtac agcaattaaa gctaaaaagc
    46981 atcggaaaga tcccattaca gcaaggttgg caggaaatct tacgcatcaa gtcaaaaaag
    47041 aaagaagctc agactcttcc tcctatcgta gaaaatgaag aagcagaagt agaactgaaa
    47101 agtatggaga aagaaacaca acctccaaaa ccctataccg aaggaacgtt gatcacagca
    47161 atgaaaactg cgggaaaaac gctggataat gatgaagcgc aagaaattct caaagaagta
    47221 gaaggaatag ggacagaagc tacacgagcc aatatcattg aaaatcttaa gcagaagcat
    47281 tacatcgaag tcaataaaaa cgaaataact gttacgccaa aaggaatcac tctttgtaag
    47341 gcagtagcca atgaaccatt actgacgagc gcagaaatga ctgcgcgctg ggaagggtac
    47401 ttgagaaaaa ttggtaaaca agaggggaca ccatctgttt ttttagagaa tatcaaaaaa
    47461 ttcatcctac atttattggc agaagttcct aatcaaatcg acaaggtaga cttttcagat
    47521 gagattagcg ggacacgtca gataaaagcg atggagaaga aaaacgatca attaggaaga
    47581 tgtccaaaat gtaaaaaggg aatagtcatg ctttatccta aaatcgctac atgcttgaat
    47641 ccggaatgcg acttcaaatt gtggccaaca gtagctaaaa agaagctgac taaaacgaat
    47701 ctcagagatt tgctttcaaa aggaaagaca agtaaagttg tcaaaggttt tactggtaaa
    47761 aaagggaaat tcgatgcagt tttagtatta aaagaagata tgacaatcgg tttttctttt
    47821 ccatggatcg aaaacagtgg aacaaaagca gttgatggaa acaaagaaac atcaagaaaa
    47881 aataatgaaa acgaagtaga gtaaatgaaa acaatgaaat agcggaatat ttattccgaa
    47941 aagtgagaat ataaatacca ataaaactaa aaaatatcag tgaatcaagc aaaaacgttg
    48001 gggtaggctg attttatata gatagcaagg agttttttta tttgaaaaaa gtgcattcat
    48061 ttttaaatta tcttatggga gcaatcacga ttgtggctgt gtaccaagca tttctaaagt
    48121 atcctgatta cgggacgaag ttgttgtcat tgacgcacaa tcaaacagca tattcaccaa
    48181 cgttgatttt atcgactttg actgcaggag ctaaaagcgc tacttatttt tttcataaaa
    48241 cgacaagcat gctaaagcga aagaaacagc gaaaacaatt gttggtggtg gtagcggcta
    48301 tgatcattat ttattttcta ccagttatta cacaagtatt tcatcaatat tttggttaag
    48361 aatacgagag tttgtgacaa aagtcatgtc acaaactttt ttctttgtca ttttttcgta
    48421 agaaaaagca aaataaaagt atatacattg attaaaagta tatacttgga tataatgtac
    48481 ttactataga atgagaatgg tatagagggt ggcgaaatat gaaaaaaatg aaaaagtata
    48541 aaattagaaa atctggcaat agcgatgtaa caactatccc accagaagta aaagaattca
    48601 tgggtgtgca gacaggtgac gcaatcagtt atgtttttca atctgatggt tctgtacgaa
    48661 tgatcaaagc acaagaagaa cctgatattg attcattagt tgattcgatc atgaatcaat
    48721 atgaagacgc gttaaaagat ttggttgatt tatgaggtat ctttcgacta aagaaattat
    48781 caaaatcaac gcgaaagtta ttttacgtca ctcgccaggc gaaatgatcg gtgtaaaaga
    48841 tgcaaatgca ttagatatgg ccgtcaaaca gccttcacaa gcagtattca atcaagaact
    48901 ctatcctgaa atctatgaaa aagcggcgat tttagcaatt aatttagcta aaaaacatcc
    48961 ttttcataat ggcaataaac gaactgctct tgttgccatg ttgttgtttc ttcaattgaa
    49021 taattgtcca gttgcttttt ctcgtcaaga agctgttgat tttatcataa tgattaccac
    49081 tagttcattg gatttcgatt cactaaagtc caagataacc agttatttac gacaaaccgc
    49141 aataaaatga acgaattaaa aactttattt ctcaattaga tcagcatgtc attaaggaat
    49201 atgctgattt ttttatgttt aatcatacaa aatagttttt tcataaaaat atggaaaata
    49261 tggattaatt ttgaaataat tgatttaaaa ggaatacaaa agagtgaata gtaggaaata
    49321 gtggtttaga actagtgggc tgatttttta tatattttag tttacataat atatattata
    49381 caaagtagat ttaaaaaatg ataaacctct ctcgaacggt tttttatcaa aaatcgtcat
    49441 ttttccatca aaatctgctt atgcaaaatt ataaaaaaat tttgcagttt ttcttacttt
    49501 ttttgtcgtt taattgaatg ataaaaacca gtttctttat tcagttaatc aaatatgaac
    49561 gaattagata tgaatcattg tatttgccgt tgcggaagga ttttttaatt aaccacagat
    49621 aaacattgat aacgattcgc ttattgtaat caacagatta aatttcatat gttcatatgt
    49681 acggatacat tttcggaccg attaatttta accattctgt ttatttgctt acctattaga
    49741 tatcaataaa aaactgctta acagctattt cagctgttaa gcagttttag gatttttagt
    49801 tcatattcat tgtttcttat aatacagtgt tcaatacttc tcttaaagca cttgttgatt
    49861 tgctgaacat tgctttttct tcttcgttga ttgataattc aacaacttca cgaacaccat
    49921 tttcgtcaac gattgatggc acacctgtaa agatatcttt ttcgtcgtat tcaccagtta
    49981 aataagcaga tacaggcaat acagcttgtt cgttattcaa gatagctttt acgatacgtg
    50041 cagtactcat accaatacca tagtaagttg cttttttacg atcaatgatc tcataagcag
    50101 cattacggac tttatcagca atcacatcta attcatcttg ggcaatacga tggtcttttt
    50161 ctacaatttc aaacactggt ttaccaccga cagttgtatg tgaccatgca gcaacttctg
    50221 aatcaccatg ttcacctaag atatagccgt ggacgctgcg tgggtcaact tttaatttca
    50281 atgcgatttc tttacggaaa cgagttgtat ccaatgttgt tccagtaccg ataacacgtg
    50341 atgttggcag accagattcg ttccaagcga tatatgtcaa aatatctact gggtttgaag
    50401 caacaacgat aataccgtcg aatcctgatc ccatgatttc ttttacgatt tggcgcatga
    50461 ttgacgcatt tgttttaact aaatctaaac gagattgacc aggtttttgg ttgataccag
    50521 ctgtgataac aacgatatca gcatctttac attcttcata gccacctgac catacagcca
    50581 cattttcatc gccccaagcc ataccatcta aaaggtccaa tgcttcccct tctgcttttt
    50641 cttgattcac atcgatcaat actaattcat tagagattcc ttggttgatc attgcataag
    50701 cgatacttgt tccaacaaaa cctgtaccga cgattactac tttacgactt gtttttttca
    50761 tgaataaaca gctccttttc ataactattt gttactttca tgaaaagtat agtcttgttt
    50821 attcatttta tcaacatgtt aattataata gttgttatta taatttttga tggaaacgaa
    50881 ttcctgaata cgcataacag ataaatgaca aatgtttaca cctatatccc ctgataatac
    50941 aaccaaaagt ttagaactga aaataaatcc aaaaaaattc gctgcaacga tataataaga
    51001 cagcaatcgc acctaaaaca attttccttc atatgcttta gtgaaagatt ttcttcttaa
    51061 tgtttatttc tccattcatt gggagtacat gttgaatact tcttgaataa tttgaataaa
    51121 gaagtcgttt gtttatatcc gatacgatta gcgatttctt ccaacgaaaa actcgtttct
    51181 tgaatcagtt ccaacgcaaa gtcgaatttt ttctttagaa ccagttcttg aaaggaagca
    51241 tcaaaataca ttttcaattt attactaaga tagaccggat tgtagccaaa atgagctgct
    51301 gtttctttta acgtacaaga agtaaaatgc ttatcgatat aatctaatac tctaagcatc
    51361 tcattttctg cgcatccatc tgctgatgaa aagctttgtt gttctaataa atgagtcaat
    51421 tcgatcagta atagtgatgt atagagattt aacgaacgga tcttatgact gtatgtatca
    51481 aaataaatca acagcatatt ttcaatcaaa tgcgtcaaac gacttgtttt tccgccgcga
    51541 aaatgaatga attgtgtatg ttcccctttt tcttttgatg catttatcaa aaaagaagaa
    51601 agtagattat ttgtgcttgc caaatgattc aatatctctg tagataaggt ttcttctttt
    51661 aataagatgt tgattaaaat atcctcttgc cctgtgtatt cgattctttg agtagcttct
    51721 ttatcaatga tcaccacgtc actttcttcc atctctatcc tttctccatt aataatttgt
    51781 aaacatgtac cgctatacat ataattgatt tcaacaaaat catgtgtatg ctcgggaact
    51841 tccgcatatc gacggtgttt actgatatga atatttccct gttgaaatag atcccctcgg
    51901 tggatttttg gcggtgtctg aagatggtaa gctaagttta aatcattgat attcattcct
    51961 tgttcacgct gcagagtttc gattgattct tcgttacgca aaaaaaatgt ctaattcagc
    52021 tagttccaat tgactccctc ctttttgtaa gcggtttctt tcgcttatta gttcacgctg
    52081 tactgctatt atatgcaaag ctatttttgg tttcataaag actttctatg gttgggacat
    52141 gaacttcctg tgattccgct attgagagaa taaccacttc atactatatt tttgataagc
    52201 gcttacaaaa atgaaaggaa catgcttatg aataaaaaga tgatgaaatt ttctctgctg
    52261 tcagtctctc ttattttgac ttctgctggt gcagtcacgt ctgcaattcc cagtatgtca
    52321 gctacttatg ccacgcgttc tttatctgct gttgaattat taacgactgc tccctctttt
    52381 atggtcacta tttttgtttt gattagtagt tttattgcta ggaaaatcgg acaaaaacaa
    52441 actgtgatca tcggattagt gattgcatcg atttgtggta tcctccccct cttctcacaa
    52501 agttacagta ttgtcttagc ttcaagagct ggactaggta ttggctttgg attgatcaat
    52561 tctttggcag tcagcatgat tgctgccttt tatcacggag atgaacgagc ctctatgatt
    52621 ggttttcaaa gtgcatttca aggatttggt gcagctttaa tgacatttgt tgcaggacag
    52681 ctagtacagt tcggttggca atataccttt ttagtctacg ccatcagctt acctattctt
    52741 gtcttgtttg ttttgtttgt tcctacacct gaaacaactg aacttcaatc gcatggcaca
    52801 gccccaacaa gacaagtaat caaaacgaat cctaaaatga ttagttatgg tatttttctc
    52861 ttgctggtca tcgtggtata taatgctgta tcgattaaat tagccacagt cctgacgact
    52921 tattctctag gaaacgaaac taatgcagcg aatatcttaa caatcatgca attggcaagt
    52981 atggctgccg gagtttgttt tggctggctt cagacaaaag cacatcgcta tatgcttaca
    53041 ctttctttac tactgatggg aatcggcttt gtattgattg ccactgtgat aaacctgtat
    53101 gtgattgggt ttggtgcgat cctaacagga atttcttttt cgttattcat tccttactta
    53161 ttcaaccaag taagtatcca agcgcccaag tacgcggaga gtttcaatac ttcaatctta
    53221 ttggttggcg ctaatttagg tactttcatt gcaccatatg gtttgctctt tctctctttt
    53281 gcttctcttg ggagtaaaac acttgctgtt ttcgtcaatg gcagtatctt gctgttgatt
    53341 agcgcgctta ttagccttct agtagtacac aggacgaata gaaagacagt gataagagta
    53401 gatgaagaaa taaaagaaca attgacaaca aaataaattt gatggaggtc gtgtaaaatg
    53461 acaaatgaaa aacataaaga tagatggctt tggtatcctg gagactttga gatccgccat
    53521 ggactgctac aaaatttcca acgggaagaa cgaggttttg actggccagc gtactggtac
    53581 atggatgatt gccatcggaa cgtcaagttt aagagatatt actttcttga tcaaccttcc
    53641 atgtttaaag tgacgatcca aggagttggg tatgtagaaa ttaatggaca aaaacatcca
    53701 tgtggaaaat ggctgacttg tccagctggt aaagcaaaaa ttcgtatctt tgtaggacat
    53761 acatcagggc taccagcgat gtttatcgag ggagacgaag taaaatcaga tattggctgg
    53821 acagcaagta attttattga agaatatcca gctggctgga gtccgcttta tgtcgatatc
    53881 gcaaaagatc caaatcaaat ctattatcaa aaagaatgcg tccatccagt cactgaaacg
    53941 gagattggcg gaggaatctt attcgatttt gggcgagcag tgaatggctg tgtagaacta
    54001 atatctctgg atgaccatat cgaatctatc actctatgtt atggagagtc cgatgtagaa
    54061 gctatggata tcgattattg ctactataag caaataggaa tgacaagaaa aatgaaagct
    54121 cgtaaacgcg cttttcgcta tttgtttata ccagaggttt ctactggaat ggttcaactt
    54181 caagtttttc atgaattttt atctctttcg aaacaagctg ctttttcaag cgattcttct
    54241 ttactgaatc agatctggtc agtatcagaa gaagccttca agttatgtag tgatttgttt
    54301 tttatcgacg gtatcaaacg agatcgctgg atttggtctg gagatgcgta tcaatcattc
    54361 atgatcaatc aatatctttt ttttgacgag gaaatcaata aacgaacgat attagcatta
    54421 agaggacaaa atgaaatcaa gcagcatatg aatactatcg ttgactattc gatcttgtgg
    54481 ttgattggta tcgaaaacca ctatatgatg acgaaagact atgaattttt acgcatcgtc
    54541 taccctaaga tgcaatcatt aatgagatac ttgatggagc aaacgaatga attagggttt
    54601 atttatggga gagcaaaaga ttggatattt attgattggt ctgaaatgga taaagaagga
    54661 acgcttgctg cagaacaagt cttattactg aaagcctatc attccatgat cacttgcgga
    54721 gatatcttag ctgaaacata tggagagaac gaaaattatc atactcatct ctatcaagaa
    54781 aaatatcaac tattgaaaca aaacctattt gactactttt gggatgaaga acttggcgct
    54841 tttatcgatt gctatgaatc tgggagaaga aatgtgacgc gtcatgcgaa tattttcgct
    54901 gttttattcg attttgctaa caaagaccaa cagcaatcga tcttaaagaa tgtcttattg
    54961 aatgacacta tcacacagat tactactcca tatttcaaat tctatgaaca agatgcttta
    55021 tgcaaactcg gacagacgaa tatcgtctac caaacgattc ttgattattg gggagggatg
    55081 ttatctcatg atgcggtaac tttctgggaa gaatacgacc ctactcaaac aggaaatgaa
    55141 aagtacagta tgtatggaga tccttatgga aaaagcttat gccatgcatg gggagcaagc
    55201 ccaatctatc ttttaggacg ctattttatc ggtttgtatc caacaaagcc aggttatgaa
    55261 gaatttatga tacaacctaa tctagattca tttgaaacac tgaattgtac gctacctatc
    55321 aaagatggaa agatttcgct tgaaaaaaca aaagataaga tcgtgattac ttctgataga
    55381 acaggcggaa cagtgaaaat gaatggtaaa agttatcctc ttctagcaaa acagccactt
    55441 gtcttaacaa gctattcaaa aataaatgca taaaacaaaa aactgcttgc gacatatcag
    55501 aagactttaa atcttcttct gatattgtcc aagcaatttt tattttgaag tttgcttaat
    55561 tctgttcttt agaaacaatg tttgttcttt ttccggcaaa aagaaagcta gataatacga
    55621 caagtaaaaa gaaagcgatg attccaactg tcaaggctat ttgaccgttt tctcctagtt
    55681 tcccaagtac aagtcctggt actagataat cagtatcggc aaatgtagaa gaagcgattt
    55741 taagatctcc taatactgga aggataaata atggcagcca actaatcaat aagctattga
    55801 aagcagaacc taataccgcc cctcttctac cgccagtcga gttgccgaag acaccagaag
    55861 cagctccgca gaagaaatgt cctactaccc caggaatgat aactgtagtt tgcaagccta
    55921 tcatagcaaa catggatagt gttcctacaa agaagctaac aaaaaagcca attaatacag
    55981 cattaggtgc atatggaaaa acgataggaa catctaatgc tggtttagaa tcgggtacga
    56041 gttttttagc aatcccttgg aatgctggaa cgatttctgc caacaccatt cgaacccctt
    56101 gaaggacgat aacaaaccca gctgtgaatg ttccagcttg gatcagtgca aagattacag
    56161 cgttgtctcc attgcttagt tctctttcaa caaatccagg accagctaac aaagcaagtg
    56221 cgacatatac gatcgacatc aagagcatga tcgatacagt agaatctcgc aaaaagctga
    56281 aacttttagg aatctttatg tcctctgtag aacggtcttt gtttccaaaa aactttccga
    56341 tccatccact agaagcataa ccgatatttc cggtatgccc taacgcaact tgattgtttc
    56401 cagtgatgtt cttcataaac ggttgacaaa gagcaggcgt aatagttaat agtgttcctt
    56461 cgaagacccc gcctaataag atcatccaca aatttgcagt aaaccccgcg cttactaaaa
    56521 tgactgctgt tagacaagaa acataaagca ttgcttgacc tgtaagaaaa atgaatttat
    56581 atcttgtgaa tctagcaagt aaaatattca cgatcatccc tactagcatg atcaaagcag
    56641 taggtgtacc gtattgttgc aaagcaatcg ccactactgc ttcattgcta ggaactactc
    56701 cttgagtatg cagagctgtt tggaacatcg aagcaaaagg ggttagtgaa ttactgatta
    56761 aaccagcacc gccgatcagt actaggaatc cgacaaatgt tttgatagtt ccttggatca
    56821 cttctgaaaa tttcttcttc tgaagaacga gaccaagcag tgcaatcaaa gaaacgagga
    56881 tggagggctg actgacgatt gacacaaaca aattcaacaa tttatccatt gatagttcct
    56941 cccttgaatc atttttattc ttttgacaag atcttcgtta ctttttcttt tacttcttcg
    57001 ctgtcaatga tcgattgcaa aggtacgagc ttttcatcag gtatagaagt tagcgtatct
    57061 ttcaaagaat gctcaatgaa gaaataatca gccatatcgc tagatacact tccaacatcc
    57121 gtatgactta cttcaaactc gtccacatct gctccaagat ctgcaagtgc ttgtttgatg
    57181 ttcatctcta ccataaaact tgttcctaat ccactttgac atactgcaaa aattttcata
    57241 tcattcgtct ccttttctta tgatcgctaa tatttcttct tttgttgatg cgtgtaataa
    57301 atcttgaaga ttttgtttat tagaaagtag ctttgttaaa tttgccaaag cccgaagatg
    57361 tgcctcatta tcgatagcag ctaaacagat gaacaaacta atgggatggg ctggattatc
    57421 aagcagatcc acaggttgtt gaagtttcag caaagacatc cctacttcgt ttacgccatc
    57481 ttctggacga gcatgaggta atgcgatgtg atcgccaata tggataaacg caccatactg
    57541 ctccacgcgc tcgatcatag ctttgatata actttcttct attttatttt ctgataatag
    57601 cggctgtgcg gcaaatgtga ttgcttcttg ccagtttttt ggttgatccg ataattggat
    57661 catttcttct gttaataatt cagatagcat aggacgttga tcctccttct tttctaaaag
    57721 tttgttcatt ttacgattta atacttgata gagcttttct tcgtctacgc cttcttttaa
    57781 ttcgatataa ggcagtagcg ccgacaacaa ttcttgggca gaaggcattg aaaatcctgg
    57841 tatcagccaa tctctttgta cttgattgat cagctgattt ttttccaagt cagtcatgat
    57901 tggatgcaga acatagagag actttttctt atcagtaagt gtgagaggta cagtagaaaa
    57961 aataatatcg taagaagttt cgtcaacctc gtgtaagcgg tcagctgaat tagtctctgt
    58021 aaaaaagatc atcgggaata attgctgaag ttcacttttt aagatcaatg aagagctaat
    58081 gccacttgga cagacgatca cggctcttat ttttctattt ctttcttctg cgtccgcttt
    58141 tctgatctct ccgccaaata gtatagtgaa aaaaccaatc tcttcttcag ggatgctctt
    58201 ccctattcgt ctttctaacg gaagtaatgc ttttcttgtc atttcgtaga tggaagcata
    58261 ttgatgctta atttgatcga tcatcacatt aggtaaataa aaagaatagg aaatacgaaa
    58321 ataggcaggg accaagtgat aaaagacatt catcaataat tcacgaaacg aagcaaattc
    58381 tacggccatc aggaattcca tgcgatggat gacttctgaa gaacattcca gcaaaaaatc
    58441 aagtgcaggc tcctggacat ctccttcaac gatcgatgct aagcaaagag agaaaaatat
    58501 aatctccgaa ttttgatgtt cggaatagcc aaggccagtc aatacttgtc gactaatgtc
    58561 agtgactgca agagaagcca acagttcagc atcttttacc agttccatgg atgtttttcc
    58621 aattcgaatg gttgtcaaac tcaaataata agctaattca tccattcgat ttggtgcgaa
    58681 tcgaagatct gatagtgaaa tcgttttatc tagcaaatct ctaactttgg cgtaataatc
    58741 aatggaaagg gttgcggtta aatacgtgag cccaaatctt ccagcaagtg actgcatcaa
    58801 acgagcagtg cgattttttg cttctcttct taatattgct tctgacccta gcagcctaaa
    58861 accagttttc ctagtatacg acagctgaca atcttccact tctaattcat ttcttatctt
    58921 tttgatatct gacaggactg tccctttaga aacctgaaga aaatcttgaa aatgaaaaac
    58981 agaaacagac gttgcagtcg aataaaccat caaataaaga agatctcttc gctcttgttc
    59041 ggtatacaca gcttcaaatc gagtaatatc caaaaatttt tctgcccagt cgattgctcg
    59101 gaatttcttt atacggatac gggtatcatc cacatattcg gcaaaaggag ttaattgttt
    59161 gatcgcctcg cttgtattta cttcgatgat ctctgctgct ttttctagac tgatttctct
    59221 tggtgttttc aggagataac taattttgta gttcacagcg ctgttcacct cctacttata
    59281 ttctagtatg gtagcgcttt taaatcgagg ggtgtttggt acaacttaca gttggatttt
    59341 ttttacgact gtaccgaacg atttctatcg aatggataca gaaaaaaagc aggtgaccaa
    59401 atgtctcctg cttttatgaa ttgatttcgt ccgatcaatg atgatcatgt ttattttttg
    59461 aggtcagttc ttcaaatttt tctttgacat cttcaaccgc tttttcgata tctttttcaa
    59521 agtgattggg ctcattaggc tttgtttcct cttctacaga atttgaacca gataaagctg
    59581 tttctttatt ttctgtttca ccagagtttg tgccagctaa tgattcttct ttgtcttctg
    59641 tttcaccaga attagttcct gctaaagcca cttcttcttc tgttttaggg gccattgaac
    59701 ctcttaatcg tttgtgtttt tcaccgtcat aatttgtcat gttggttcct cctagtagtt
    59761 ctatactttt tcatcgtaca ataagacagt ttatgcgtca aaaaacaaac ccgattctta
    59821 caaatatgtc atatttttcc ttctgattct tgaatgcttt tgattccttc ttccaaaatt
    59881 ttcatttgta agattgtttc ttcatctttg atgacttttt ctgcgccatt aactaatgtc
    59941 tcgtaaatcc cttcgtagac acggctgtaa tcaccaacct cggaaattac tttttcttca
    60001 tgataactcc cttgttcatc tatataggtc aatacaccat aatgttcggg caaatcgata
    60061 ccaaagtctt catgatcagg catgtaaaac attttcagat gttcttcttg tcgatctttt
    60121 gtttgtttca cgaacatccc tttttttccg taaacgacga aacttggacg ttctttgatg
    60181 cggaaataac tggattttat cgaaattttc atatttccat agaaaaaatc cagatcgaag
    60241 taatcattca ttcgtccttt ccctaaaagt tgacgtacat catagctgat atcatctggt
    60301 tcgccgaaat aagaaagaac ttgatctact gtatgacaag catgtccata taaatagctg
    60361 ttcgcagtag aaaattcatg cgtattttct ggtacttccg gtctgaaata atcataatgc
    60421 atttctactt ctaaaatatc ccctaatttt ccgctttcga ttactttttg gactgtcaag
    60481 aagtcagaat cgaaccgtct gttttggtaa cattggataa atagaccttt ttcttttgct
    60541 aatgcaaata actcttctgc ttcctttgac gtttctgcaa aaggtttctc tactagtaca
    60601 ttcttaccat gttcgagtgc catttttcca tattcataat gaaattgact aggtgtagtg
    60661 atgacaacta attggatctc aggatcatcc caaagatcag acagctgatc agtatattcg
    60721 atgtcttcga atgttttcca cactggttca gatggggaaa aaattttttt gatattgatt
    60781 ttgttcgtaa gttttcttga aaaaggcaaa tgatagcgat ttgtactctt tccattgcca
    60841 agataagcga ttgtaagcat gagtggtgcc tccttttatt gatgttttca gtatatccta
    60901 gaaaagcgca gaaagtattt ataatagcta ttctggtaaa aaagttcaga aaaacacttg
    60961 ttatcattcg gtttggtgaa attaatgttc aaagtaagga taaaaatcaa aaagatagta
    61021 gcggaaccaa gtgtttatca gtaagataga atttggaggt cagatcatgc ttattgaaga
    61081 aaaactaaca aaacaagagt tgtttaccac gacagagaaa cggattgtcg attacatccg
    61141 taggaatatc gaagctgcag tctacatgac gattgaagaa ttagcaaaag ctacgtatac
    61201 ttcccattca gctatcattc gtttatgcaa aaaaatggga ttcagcggat ttaaagaatt
    61261 ccgtttcgag ctggggcggg aagttcatca attgattgct cagttcaatc aaacgattga
    61321 tcctaatttt cctttttcaa gtgaagatag cactcagcaa attgcaaaga aaatggcgga
    61381 tttatcgatc cagtcaataa aaaaagcaca attgcagatt gaacatgaag atttagatca
    61441 aatcggtaaa gtattgaaca aggcacagcg aatctttctt tttgcaaaag gtgattctca
    61501 aatcacggca aggaaattcc agaataaaat ggtcaagttg aataagtttt tgatcatggc
    61561 tgaagaatac agtgattctt cttggaatgc agccaatttg actagcgagg attgcgccat
    61621 ttttattagc tacagcggtc gcatccatca ttatgaacga ataatgacct atttgaagca
    61681 tgtagaggcc cctaccttgc tgattaccgg aaatcagcat tcagaaatgg ccaaacaggc
    61741 tgatatgtgt cttgttatat cgcaggagga atatgatttt gctaaagtag cgactttctc
    61801 ctctcaaatt gccttcgatt acgtattaaa tacgttattc tcagtgatat attctcaaaa
    61861 ttttgaagag aatatccgca acttgaaaaa caaacaagaa ttgatgcaaa aaggccttct
    61921 taaagaatat cggaaaactt aagaaaagat cctcatttag taataagaaa gatagtgttt
    61981 gataggctct ctttgatatc atcataaaaa caggctgaag ttttcttttt caaacctcag
    62041 cctgtttgtt tacggaacga ttcgaataga tatattatag aaatgaggaa tagctaactt
    62101 ctgtttctta ttttgaacga ttgacgaaaa agatatataa gaaggaggaa aatcagtata
    62161 catgcagcta acaatccata caggaaaaat cgtttgttct cggatacttt ctgtacttct
    62221 ttttgaactt cttttgcatg ataaggaacc cggtgccctc tgacaagtaa gcgatgactg
    62281 ttgaccatat aaggtgtaca agttaggagt gtcacgaaat cctgcccttt ttcgattctc
    62341 agatcttttg tatcagtcgg ttctaccgtc ttgatctgat cgacttgata agcgagtgtc
    62401 cgtccattga tttcgatata gaaagaatca cctaaagtca gtttcggtaa gtctgtgaac
    62461 aactttgctt gaggcagtcc tcgatggctg gaaataacgg catgtgtatt ttctcccccc
    62521 gtaggataag acgttccttc caataatgag gcaccttttt ctagcagcac agtattcgtt
    62581 tcatcaaaaa ttggcaaatg gacattgatc tcagggatcg tcaaaatacc gatcgtatgt
    62641 ttctcaaaat aggttctatc ctttttcgca ggttcttttt tcttagtaaa gggatctgca
    62701 cctggatttc caccttcttt tgctaattgc tggtttttct ttgtcatgtt ttcttggatt
    62761 ttagccatga cttcttcatt ttctttgacc gcttgttgct gataatgaga gatgatctgc
    62821 tgatccagat aattattcag cgcgtcgcta acaaatggat aagctagtat ccctattcct
    62881 gagagaagca agaaaagcat cagcagatct agaagtttcc ttttctttgg tttcatttgt
    62941 gttctccttg tgatgactgt ttgataataa aagaaaaagg agcatttgcg taatccgctg
    63001 ctcctttttc caattcttgc aaaaagacta aatctgactg tgcttgcgtc tagcaaagta
    63061 cagtccagca atcagtagaa ggactactcc tgcaccgata tacacataga ttcccttacc
    63121 gcctgttgaa ggaagtgtac ctttgtgttt atttggtatt ttttcaggag aaatcaactg
    63181 tcctgctgtt acataagatt gttcatcgat agtaaatgct acacggtttg ttaatggaac
    63241 atatttttct ggcgctttcg tttcttccag atagtacgtg ccatatttta gacctgtcac
    63301 atcgattaaa ccgttacttg tggttgtaaa aaccgttgct gattctttcg ccgataccca
    63361 gctgacggct tttgtggatg ggtcgatcga taaatatttc gctgtgtcac tatcttgatc
    63421 acgaacgacg aattctgctc cagcaagtgt ttggtctgat gtaacgtcac catctacttt
    63481 aacaaatcgt ttgcccccag taacgacatc gactgacggt ggtgtttgat catttgtatg
    63541 cccgttatcg acatttgctt ggttagaaaa tcctttggtt ggatctgctt tttcgttcaa
    63601 atgcatatag taaacgaatt tcaatgtacc gccaggagtt aatgaaggaa tataattcgg
    63661 atcaactgaa accgtgaatc cgtctgtttg ctcagtgaca gaataattta ctgggtcgat
    63721 ttctttattt ccatcatata aggcataagc atacgttcct gaagaatcat tatcaaatgt
    63781 taaagcagcg tcatgagtat cgataagttt gaatgttgtg tacttgtttt gcgttccttc
    63841 tttatcagcg atacctaatg ggatattgac agaaatctca tattgaattt tttcaccgat
    63901 tgcgacatct tttccattca attgaggtgt tgttttatca actttagacg tatcattttt
    63961 gatggtcttt tctactggtg tttggctatt tgccaggatc tcaaaaggcg tgattgtttg
    64021 cttttcaatc atttccgcat tatcaggagc ttttacttct tctaaattat attttccaac
    64081 ttctaaatga ttaacgatca actggccttt ttcaatagat gcctcggcaa agtcattgtt
    64141 gccgatgtca taagaatgac cagtaatgaa atgtttggct ttggtttgat cagttgtcca
    64201 agtgtacaat ccatctgtga cactttggat gtattttttg acgcttggtg ttccttcttc
    64261 tttagaaata ataaattctg ctccatttag tgcttcgttt tcggcagtac cgatttttgt
    64321 aactttcaac gttccatcat taccgactgt atttttaggg tagagatgga tagtatctag
    64381 ttcttctgtc ccgtatttat aagagccatc tgcttgtttg atcatctcat atacagggaa
    64441 agctaaaacc atgtttgcgg cagctgacac tccgtctttt ggttcttctt tgatcgtgta
    64501 gactgcatct ttcccatttt gttttttagg taaagataaa gtgacatttc catcagcatc
    64561 tgtcgttcct gaagcaactg gtgtaccagg agtcaaagac tggactgctt gttttgctgc
    64621 atccacggac gctcctttat ctcgttgcgc ataaaattct tgagtgacgt tataaactga
    64681 aaatgaaata tcggctaatc cttggtattg atcgaattcg ctcatttctt tcccgctgtt
    64741 ttggattaaa ggatcgggta aatcagtcat tttcttttta tgaaggatga cttgagctgc
    64801 tgtctcctct gcaaacactt tttttgcgcc gaagctgttt gtgagtaatg gtaaaataag
    64861 gaaaaggact cctaacataa cgtttatttt tttatgattt ttcatttttt cactcctgtt
    64921 ctttttttga taaacaacca gattgcaaag cttgcgaata ccataccaac aacgcctaaa
    64981 agtgtatttt tcgtttcgtt tgtttttgga agaaatggac ggctattttc ttttttattg
    65041 actactttca atatactgtc cgcctcttta tcctcagaaa gaaaagttga aatggtaaac
    65101 ggaatttctg tttcacttaa aatataacca gaaggtgctt cgatctcttt taatcgataa
    65161 cttcctgttt tcaacccgga aattgaaaaa gcgccatttt tatcggaaat caggtgaagc
    65221 gcatctgatt cgttctttgt ccacttgtat ccgttggctg tttgctggag gtaatttcct
    65281 gctttgtttt taataaggaa tactgcgcca gctaatcgtt tatctggttt atttgtctcg
    65341 attttgacaa attgcttact ccctgtatat acagagactt tctttgtact gacaccaaaa
    65401 ccaaaatcca gtgttccgtt gttgttgatt tccttgttcg cctgtgctgt actgcttagc
    65461 tgcatctgat aagaaatggt cagttttttt ccagcatatt tttccaacga agggatcgaa
    65521 aataaaacat caaatccgtg aggtttcttg tgtatgacaa agccttctgt cagcttttct
    65581 ccattcgatg aaatcgttaa actattaggt aaaaacgtta atgcttcatc cgcactatct
    65641 gacaattcga acttttgata gtcaaggata tttacaggaa ttgtcgtatg caaggaataa
    65701 gtgatttttt caccaatcgt aaaatcattt ctaggctctt ctaatacctt ctcaaaaggt
    65761 ggatcagggt agtcgttttc ttcattttta ggatacaaat ggatagttga aagtttttga
    65821 ttgttttgtc catgtacagg aagaacaact accatattct ctgccttttc tttgacgact
    65881 tctggtactt tggattcaat gaaaagatac gctttatctc ttttttcttg atctttagcg
    65941 gccagagaaa aagaagcgat accatcttcg ttgttaagag ttgtagttgt tgcttctgcg
    66001 gtaaacatac cggaagacgc accgtttttt gcgatctctg cttgtgcttc ttctaccgtt
    66061 ttgccctttt cccgtagatg gtaaaaagaa tctgtgacat cataaacttg gaatgtgaca
    66121 ccatttaatc ctcgatacgt ttgtaataaa gctttttctt gtccgtcatt tggatgattt
    66181 ttcggcagtt gcccgtttgg gaaaagcaat ttgtgtaaag taatctgaac catttctgtt
    66241 tctgtttctg ttgctacctg agtaaaagct ggtttaaata gtaacaagaa gagacacata
    66301 ctaagccaac caagtttttt catatttagc tgccccctct ttcttttttc aaaaaaagat
    66361 accaaataga aaacgcacaa ccaatcatcc ctactagata gattcctaaa cggccaattc
    66421 ctcccgtttc aggtaatggt acttttgcct gattcgtgat gtctaaagaa atctggttgt
    66481 ttttggctcc tggtgacaga acagattcat gatcttgtcc atccacttga attgacccat
    66541 cttcgtgtat aactatagta actggctctt ttagaccttg gtatccttct ggtgtaaaag
    66601 tttcggtcag cgtataagtt ccaggtttta aattttcgaa tagaaaggta tctgtttctt
    66661 gtccatcttt tggcgattct aagtccattt ctggtccctg cagtctgaat tttgctcctt
    66721 ttaatgtctg tccgttgtct tcttttttgt gtaccgttag atcaaacgct tttagtcgat
    66781 tttgatgagt gagcgtccat acattatttg tcatctcggt attttttccc gtgtaaatga
    66841 tttttccata tttatcgatt tggaattcat aatttcccgg cgaagtatca tatccggcag
    66901 gaccagctgt ttcaacgact cgatatttac caggtgtgag tgaatcaaaa ctggcaagac
    66961 ctttttcatt cgttgtttgg ctgtcaattg gctgataagt accatttgct tcttttttct
    67021 gtagggaaaa tgctgctcct ttaagattta tctctttgtc cgtcccttgc atcgcatact
    67081 tacgtactgc tacaggaact tcaacaaaag gattttcaat agtcaactgg atcgtatcat
    67141 cggaagtagt gactgttttt ccatcaatgg ttaccgtacc atcagaagcg atcttgattt
    67201 cccaagtagt ctttttgctt aatccatgcc cttctggagc ttttgtttct gtcagcgtat
    67261 aggtcatttc tttttgcaat ttgacatttt ctggaagaga ataggttccg tcgccatgat
    67321 ctgttagtaa agtatcaata gaatctcctg ttaatttgaa aacagcgcca ataagatcct
    67381 tttcacctgt agaggaattt ttcgttattt tcaagtttaa cggtgtgaat tgtttagtat
    67441 ttttccatgt cattgcatct atttgttgag aatcgtatcc tggtacaggt aattctttga
    67501 ttgtttggta actgaatgct tgaccttgat tattgtattg aggtagtgat aatatctctt
    67561 gataactttc tccgctattt gcagataatt tgtcaatgtc tgcacgttcc cacgtatttg
    67621 ttgtatcttt agctggttta atgattcgaa tatatccgtt tttccaagct gcagcatttg
    67681 tcgtatgttc ccgttgaatc tcaaaagtaa cttgatctgg acgatcagct agattgttgt
    67741 caaattcttc ccataacttt ttgatgtgaa gactgactcc gggagcttta gcagatggta
    67801 taccgaattc agctaattca ttggtatcaa tgtttggctg gaaagttgtc ctgccgttca
    67861 tttgatacca gaaatttgga tggaagtcct cattttctgt ttggattctc acttggtaat
    67921 ggatttggat ttcttggtct tttcctaaat agatctgatt gctcttgatg gtatttcctt
    67981 ctatggaaat agatggagtg accgttgtag gacttgtccc cacactcttg actgaaagtg
    68041 taccaggctg ataaatgaat ggttctgcga ttggatcatt tatttgtcca ttgcttacag
    68101 ttgctgagat ctgtacagct tttttggcta ggtattcaga gatatctgtt gcatgatcag
    68161 ctgattcata gtaaagatcg cctttttcat ctgatgaaac catttgtcgc atacgagact
    68221 ctacttctgc ttttgagaga ccagctgccg gatcgctttg aagttggatg ccaagaccat
    68281 gtatttcgat tcctcgttct ttgagtgcca tcgcttctcc gatggttgcg ataaacgtac
    68341 tgtcgattct tctggataga ttgttttggt caggtgcatc atagattctt gagattagag
    68401 aagtatttcc cggccgatct tgcgtattag aaaactgagt tccgtaataa ttgctgcttg
    68461 attgtgcgtg tacccgctgt actttatagg aaaatgttgg tacaccatcc gtcagcaaaa
    68521 cgatcacttt tttatgtcca tttggaacgg atagcatgct tcctgcatct cttagtgctt
    68581 tttgtgtaaa agtaccacca tttgtccgtg aaggtgtaat ggattttact tgatttttca
    68641 ctgaatcaaa tgaacccatc tgtactgcac cgttactgta gctatatcct tcgcttgagt
    68701 agccgacata tcccatattg attttgtctg tgataccgct atctgctaaa gtatcgacaa
    68761 aacgatcgac accaatcttt acttcaccga tccgattatt gtcgttcata cttcctgacc
    68821 agtctacgac caatacgaga tcaagaggcg tgatatcttt ttgtacattt ccacgagcat
    68881 tcaaataaac atcaaacaat ccgggtgtac ttgtttcctt tgcatacttt cgtatcgcat
    68941 agtctgcttg attaccggtt cctccatatt cgatatacga atgggtccga tcattaggat
    69001 ctccattcca agaatttata ccatcccatt gatttgttcc gtttttattg ccttgatgat
    69061 caagaacatt tgtatttcct gtaggttgcc aactggcttt aggataagtg ccctgatcat
    69121 ctgttgtata ttctgtagga atcgtttgat tagatggcac actcacagct ggcaaggata
    69181 atgtaggcaa tgtaaatggg ctgacagaat tttcgttttc ttttgatgat tcttcttcta
    69241 tttcgtctgg ctttggtact gacacatctg tatctttctt attttctgtc tcatcaataa
    69301 tgtcatattc tttattatcg acgatcagct tgtttgcctg gctgtggtaa agtgctggat
    69361 cgattggaaa actcagatcg atagtttgtt tagtgtttga tgagatttcc aacgtaattt
    69421 tcccgctatt tacctgataa gcatagccat ctccttcttt tctttccaca tcacttaagt
    69481 aattttcgac tagaccaatt tctttttcta tcttttcagc actggtgtta ttcgcattta
    69541 gcttgatgtt cagtgctttt tttccagact gatccgaaat gacttcagct gaaattgttt
    69601 ctaaaactgt tggatcatct gtagtgtctg ctattgcaga taatggcaca aagtttgata
    69661 aactcaatat tatcagcatg aaaataactt tcagtttctt ccctgtggtc gtcatttttc
    69721 ccctcctttt tctttttttc tgttttgacg tgaatagtga aaattattaa aataaacagg
    69781 cgttacataa ttttatttat tttaatatga taattattat acgaagggcg ctaaagggaa
    69841 tcacaccaat tttcatacca attggtttca tttatgaagt tgattccttc gattaatgag
    69901 cgtttcattg aaagaaaagg gttgagggag aaaaacatat gatcaggttg ttggaacgaa
    69961 actatagaaa gatggaaaaa atcattcgaa ttctgtccat ttctgacaag tggatgacta
    70021 aaaaagaact tgccaaacga attggtagtt cagaatctac atttgtaagg tatatcgaag
    70081 agatcaaaat ccgctgggga acggatgtaa cgattcaatc ttcacaaaaa ttggggtatc
    70141 gtcttgaaca tttgaatgta ccagcttatt taaatgttct gatggaaatg tcgcaaagtt
    70201 ctacaaacag tcgtttatta aatgagatta ttgtcaatcc gggtaaaacg attgagtatt
    70261 attgtgaaaa aatttccatc agccggtctt cttttacacg taagttgaaa caatgcaatc
    70321 aaattttaga aagctattta ttaaaagtga tcgttgacca aggttttcag ttaactagcc
    70381 ttaaaagtga gctccagttg cgtatctttg ttaccttttt ctttttaagt tactacggaa
    70441 gacgcacgtt gccatttcca atcgaaaaaa agaagatcaa agaaattatg gtcaagaata
    70501 attgtcagat gaatttgttt caggaagaaa gcccatatga gcaatctttc tttattatgt
    70561 attttgttgt gcatctcatg agagaatccc aaggaactcc atttgaatgg aaagctggag
    70621 actcttatgt acagttgata aatatcgata aaaaagactt cttaatcttg aaaaatctct
    70681 taggaaaatt gaagtacagc agctatataa aagtagtgaa ctattttgtc aatacgattt
    70741 tttcagaatt ttcggagaaa gaaaaaaatg aaatcagtga gcagatacga aaaaatatgt
    70801 cctactgttc gttttcatcc gctgattgca aagaaaaaaa cgaagaatat gttgttcaat
    70861 tattaacgca aatttatttt ttctcccgta ttttcccata cgatacgact catttgacga
    70921 tccgtgcaac ttactttgca cagcagatgc aacaccgcca gatacgtcta tatgctggat
    70981 ttcgtgcaga acttcaattt tattccactc tactgaatca gaatctgatt tattatttat
    71041 ctagctatat cttttggatg cttatcggta atcctgaaat tcaccattat acttctgata
    71101 aaaaaatact gattatcagc gacttaagtc ttcgacatag tcaatatatt gaagaatata
    71161 tcagtgatat tcttgctgtg cataaaattc actctgagac aactgctatt actgaagatc
    71221 agctgtcgaa atacaatctt ctcgagtatg atctgatcgt tacgaatcaa cccattttga
    71281 atgcccatgt tcctcatata ttgattgatg atagtgtgtc attcgcaaat gaagaagaac
    71341 tcatccgctt attcgagtta tgaataatca aaaaacagct atgtataaac caccctgata
    71401 aaaatttcag gacaaataca catagctgtt tctttttatt gtattccttc agcaatccaa
    71461 cgagatttat agcgaaagtc ttttaacaac tcttcttttg tccaattatt tgcaatgaga
    71521 tccgtcaaag tgttcattac gagggtcatg gaaaagtata ggaaactaac tgcttcttcc
    71581 gacgattgaa tggagaaatg attgattctt aataattcca gccatttttc gttcattgga
    71641 gaatcagccg atagttctct acgtttggtt gtctgctgat catttcgctg ggttaacaag
    71701 tataaccttt gccaatagat actttcgtga tctaagtcgc agcaccactc taaatatttt
    71761 tcgatcccca aaaagaaatg ttgtttgttt tcgctaatga attttaagat atcttgatgg
    71821 actatgtgag aactgctttt gatcgtatac aaataagcat cttccaaatc ttgaaaatat
    71881 ttataaaaag caccgcgcga catccccatc ttgtcaacga tttctgagac tttgacttgg
    71941 ctgatgtgct gtgagcaaaa tatctccagt aataggtcat cgattctctg tttctttgcg
    72001 ttcgataaat tgaaaaaagt ttcttttggc atccacatct ctcctttatc tctgattata
    72061 cgtggatagc ccttcattgt catgaaggat tcattccttc tttttctctt cttctgtcaa
    72121 gtacccatct tccattcgat aaacgcgatc actccattga atcatacgga catcatgagt
    72181 aaccataata gtcgcttttt gtttctcatg agcttcttta actagtaatt tgacgacttc
    72241 atatgcatga tctgtatcca aactagctgt tggttcgtct gctaagatca agcttggctc
    72301 attatataaa gcgcgagcaa ttgccactct ctgtctttct ccaccagaaa ggtctttagg
    72361 ataactattt ttaagtgact ggatatctaa agattgcagc aaggaatcga tttttgttga
    72421 atcttctttt tttgaagtaa cttggtcaac tagttcaaat tgttccttta ccgttaaaaa
    72481 aggaatcaaa ttagagcttt ggagaataaa tcctatttct ttaaagcgta aatctgctcg
    72541 ttttttttct ggaagctcag ttagtgagtg accgttgatc actatttctc cggaagtcgg
    72601 tgtttgcaat cctcctgaaa tggtaaggaa agtactttta cctgaaccag aaggaccaat
    72661 gatgctgacg aattctcctt cctttacata aaaatcaatt gctttcaaag ctgagacctt
    72721 tgtatgacca gaaccaaatg ttttggtaac ttttttcatc gttaatattt ttttcatttt
    72781 tattaacctc ctattgctgt aattgggtct actttggtta tcgtacgtat cgaaaacaat
    72841 ccgcctagca ttgcgacaat gatcaaaatc ccactatata gcaaccattg tgaccaaaaa
    72901 acagcaaaag gcatcgcgct tggtaaaatc agactagtca aataagcaaa caaaagagca
    72961 agcagaacgc ctaccacccc aacaataaag gactgtgcta ctaaagattt agcgatgaag
    73021 aaatttttga tcccttgtgc tttcattact ccaaaaatag cagtcttctg aagtgtcatc
    73081 acgtacatga aaataccgac cacggcagca accactaaga acaaaaagta tatcatcgca
    73141 tttaaggtca tattttgggc agaatagcca ggaatacttt caatgaatgt cccaatagtt
    73201 aataactgta gacccttcga tgctttttct ttcgaaattt tagctgaatc ctttattacg
    73261 atcccattga tcggctgttc ttcttcagaa gcaaacggct gttgtccata cttcaaagca
    73321 gtccacgtat ctagggacgc atagatgacg ggagaaactg tgtaataagt ttcaggaaag
    73381 ataccgacaa tcgtcaaagg atcgtcgtaa ctgccaattt tgatcgtatc tccaatttta
    73441 tagcctgaat cagccagatt ttgagagatg atgatttcat tctttgtttt aaattcatgc
    73501 cctttcgtga gttttggtaa taaaaatgct tctttcgttg tgccgaaaac tgtaacattt
    73561 tctttgtttt ttcctttgat tgctccgctg tacaatccga ttggtgcttt ctctcctcct
    73621 tcaatgtatt tcagatctcc tcttgttaat tgtgaagctg caaatacttg atttgcatcc
    73681 tccgacaaaa caatctcttg tgcgtcccaa tcatcgattg cttttttaaa ctcttctgaa
    73741 agtccattcg ctagtcctga caacatgaat acgacatacg caatcaagaa cataataccg
    73801 acgatcaagc catatcgtaa tttagaatag cgaatttctt tgatagctag aaacatttgt
    73861 ttttcctccc tatgatagat tgatagtgac aaaatgtcac ttgttgaata gtgtaactct
    73921 aaagtgacaa tttgtcactt gtgttgtttt tttcttatca ataaattgtc taagacatct
    73981 ttcgataaaa ccttaacatg agtcataaaa aatttattta agccttattt atgctttcca
    74041 tgataaacta aagtgaccaa atcaatgagg tgggaaaatg attaaactat tagtagtaga
    74101 agacgataaa gtattatcca ataatataaa agaaatactc gctgcaatcg gcgagataac
    74161 acaagtttat gatggtagtg aaggactgtt cgagttggaa agtggagttt atgatcttgc
    74221 aatcttggat ttgatgatgc cggaaatgaa tggatatgaa gtgttagcaa aaatacggaa
    74281 agaaaaaata cagactccag tattgatttt gactgcaaaa gatagtttgg aagataagat
    74341 tgaaggattt gaacgaggtg cagatgatta tctaacaaaa cctttctatc gagaagaact
    74401 gatcatgcgc gtgaaagcat tattgaaacg ctcgctaggt ctctttgatg aaaatgttct
    74461 gaagtataag gatatcagtt gtgatttaaa aagcaatgaa gtcttgtata aagaacaagt
    74521 tttgcccata caaggaaaag agtttgaatt gctcgtttat tttttacaaa ataagggaat
    74581 cattttgaca aaagagcaga tatttgaccg gatctgggga tttgattctg atacgacgat
    74641 tacagttatt gaagtttata tgagcaactt acgaaaacat ctgcgaagta ctggattgga
    74701 taaagaaatc aaaaccttac gtaatgtcgg ttatatactg caaagggtgt gatgcaatga
    74761 atggagatat tacacgaaag caacgtagtc gcttttttgc gacaaatgta gcagcatttg
    74821 cttttatctt ttttcttttg ggattcatta ctttacagat attaaaccaa tctgcataca
    74881 gagaaacaga tctgtcatta gagaaaataa attttgattc tcgaacagta caattagaga
    74941 tacaaagata tcagcaaggc gatccctttt taaacccgac tcaaacggaa caaaacagaa
    75001 caaacatgga acagccatta atggaaccat caaatgaccg ttttaacact caagtgatct
    75061 tgtggtctaa aaatggtgag atattgaata aagcagctat tggtgggcgt ttcaaccaaa
    75121 tcaaagaatt aaaaatggat acgaaaaatc tgaataccat ccaaaatatt gaattaaatg
    75181 aagacagtgg ctcatctgcc ctctctttcc gttcgattac gaaaaaagcg gagacgaatc
    75241 aagatggcgt tgcctacatc caaattttag aaaatacaaa tcaaatcaag aattcattag
    75301 cgacttttcg cacgattatg attttgtgta tgattgtttt ttggattctc tctattggta
    75361 tcagttatta cttgtctagt ctaagcatgc gccctattat tctttcttgg agaagacaaa
    75421 aagaatttgt tgagaatgct tctcatgaat tgcgcacacc gttaacgatt attcaaaata
    75481 gtttggaaca tctttttacc aagcctaatc atactattat tgaagaatcc gaaagcatcg
    75541 ctcaagcatt gtcagagacg aggcgcctca ctggattgac ttcggatttg ctgacaatcg
    75601 ctcggaatga ttctgatcag cagctgcttt caaagcaaat gatcaataca caagaatata
    75661 tcgaacaact tgttaaacca tttcaagaga tggccatcat agatggaaaa gagtttattc
    75721 tccataacga tgcaacatta gaagtaccaa ttgatgaaaa aaaaatccat caagttttgg
    75781 ttattttgct ggataatgca ttaaaatata cgaatccaga agataagatc attttggatt
    75841 catctgttaa ccataaagaa tggctgattc acgtaaagaa tactggccct tctatcagtg
    75901 aagaagatca agcaaggatt tttgatcgat tttatcggga agatcaatct cgggcgaaag
    75961 agactggcgg atacgggctt ggcttggcaa ttgcaaaaca ggtaatcgaa cagcatcatg
    76021 gacagatttc tgtatccaat tatcttccac aaggagtcga ttttaaagtc caattgccgt
    76081 tgaagcctaa aaaataatgc ttatccctaa aaaagggctg tgacaaaaat cttgtcacag
    76141 cccttttttg ttgcgaataa acggtgttcc agaagtagct tcttcggaaa taagccgaaa
    76201 tttctttaag ctgaccactt ctgtcacaac ctctttagct tacgcttacg attttttatt
    76261 gcggtcttca atgtgtacat caccgcttgt gtcaatatcc aattcttctc ggctaacatc
    76321 ttctgtcact ttttcaacat cttcatgttc ttctttatga atcccaactt cttcacgaat
    76381 aactgtatgt ttgcttacat ctacttgctc ttctttgatt gggatagtaa tcgtgtcatc
    76441 ttccaatttt tcgtctgttc cctgtgaaga ttgatctgtt actggtttgc gttcgatgac
    76501 gatttcttca cgtttgaccg gtacttctac cgtttcagta tcgttgacca catgtttgtg
    76561 gatgtcgact tctccagttg tcacatcatg tgtatttaca tctaattttt cttcttttag
    76621 ttgaattgta tcatcatcta gatttggttt gcgatcgatg tcttcagtct tgcttgtacc
    76681 cattgtatca gtctcgccag gtacgtccat cggatcagtt cctggtgtca tattcaatgg
    76741 atccgttgta ttttgaaagc ctgtcgtgcc acctacgtcc attggatctg ttccagtgcc
    76801 agtttctaat ccagtcccca ttgtgcttgc tgggctaaca gtatcttgtg tatccattgt
    76861 atcttctgtt gtttctggct gacgatagcc ttttactgtg atgacaaatt ttccatcaga
    76921 aatatctcct ctatattgat ataaaggatc attttctggt gtagccgtct cagaatcata
    76981 ggtatcaaaa gaaaaagtat cttttatttt ttcccataaa gaacggtctt cttcaccatc
    77041 catccgattt tcatctgttt caacatctat accagacaat ccgtcaaatc ctagtctaga
    77101 tgctgcttca tcagtagtat aaataacgat atcatctcgt tcataccctt cgttacgcaa
    77161 ccgttcaatt actgataatg cttcttccct cgtactatag cttccgatga ccgttaaatt
    77221 tttctcttcc atgtaaattc ctcctagcaa atagatttat gattggtata ttgttactta
    77281 taaatttatt ctataggaga cgattgtttt ctacaaatat ttagattgga atttgattaa
    77341 aacaatttaa ttgaaatttg aaaatattta tgttaatgaa agtcatattc taataaaatg
    77401 tcatttgttt ctatatgaat ttttatgaaa aatttcatat aatttccact cgttgtgatg
    77461 gtttaattta tccaatccat caataagcaa acaacttata taaaaaagga ttaattctca
    77521 atataattgc tcctaccata gaataaatct tcatgtaaag taatgaagta ccaactattt
    77581 ctaatacttc tttaagaatt atcccaacaa tatcgttaga caaaccatta ttgaataaat
    77641 ggagagccaa tcgattctga aaaaatgttt tctaattttt cggaaataaa agaaagagcc
    77701 tcctgccatt agaacgaacc aaatccattt cattgctcat gaagtaacca catagctaaa
    77761 aagaggataa atcaattaaa agatcaaaag aatgactgca ctaataaaat aactaaaaat
    77821 gtatcttctt acttcatatc ttttagctag tacttagtta aaaatttgct tcctaagatc
    77881 aagacaaact gtctatcttc atttgatgaa atgcggtaaa atatagaaaa tgaacactat
    77941 tggagggaat ttgtaatgga cagcaaaact agaaaacaac tgcaagaaaa gttaaagcaa
    78001 cgtacacact tgctcccttc cgtctttcaa cagtttttag aagaaaatcg ttcttccctt
    78061 tcattaggca gtcgctatga atacgcaaaa gattttcatc tgtttactga ttatttgaga
    78121 aaagtatttt ctgaagaagt ggatgatcag ctgattatcc atttagaaaa acaagattat
    78181 cagaatttct tagctaaaat ctatcgacat actcgttcgt tccaaactac agcgaaccaa
    78241 gaaacggagc aattgtttga aaatagctat tacgggattt caaggaagca atattcatta
    78301 aatcattttt tacgattttt gtatcaaaaa ggactgattg aagaagagat ttctcttttg
    78361 ggtacaagtt taccggaaac aaccaaagca gctccgcgat atatcgatgc agaacttttc
    78421 aaagattttg agcatttgtt tatcccgatt gaaggctttc aaacaagcag agaagctagc
    78481 tttgaagaaa agaatagacc gaggaacctt gcaattactg ccattcttct gtattgcgga
    78541 ttaaagatcc atgaagtagt agaacttaaa cgtaaagatg tatctttgac caagcagatt
    78601 cttaatctga atagaaaaga aaacagactg aataaagtac caataccaaa agaagcgatg
    78661 ccatttctgg agaactattt taagttaaca gcacaaaata cggacgaaaa ttcttttgtt
    78721 tttcgttcct cacatgatca gcaaatttct cctcggacta ttcgccaaat tttgagcaaa
    78781 attacagtat ataaaaacgt atcgccagaa ctatgcagaa aaacatatcg ttattatgca
    78841 gaactatatt tagatgatgc tgacgcggta aattatttat gtggtaatcg atacctctca
    78901 tcccattctt ttcaagatat ctgggaaaag atggttgatt tttcttatca cgaattagcg
    78961 gcattcgttc gggtttcttc catataaaac aaatgtatga tctttttttc acaataaact
    79021 atgttattat ataaaggatt atgttacgat tatcttgaat ttgtatatta cttaacaaaa
    79081 gataatggag tgtgaatgat ggagaccata acgtattccc ctttgaactt atttacgaat
    79141 tttcaagaag ctgcagctaa cttttccgag accccgatta tctttgatga gccactttca
    79201 gctttcccag aattaggact tgaaacaact tacaaaaaat gctgcgaaag tgtgttgaaa
    79261 cgggcttatc aattggctca tcttggagtg aaatccggag acaaagttat tatttataag
    79321 agttccgctt ttgacacata ccttctagct gtttctgtgt cttaccttgg tgcagtgcct
    79381 gtcatgactt cttatcatct accaacaaca acaatggaag tatttatcga tcgtttagaa
    79441 gaccctttca ttttatttga tgatgagaca aaaaagcgag tgagagaaat ttcaaatgga
    79501 acaaaatcaa aacagattc
//
LOCUS       DASHRQ010000005        67557 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.005, whole genome shotgun sequence.
ACCESSION   DASHRQ010000005 DASHRQ010000000
VERSION     DASHRQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 67557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.005"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(105..629)
                     /locus_tag="U2M88_000362"
     CDS             complement(105..629)
                     /locus_tag="U2M88_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8372774.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="U2M88_000363"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="U2M88_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEM8372775.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="U2M88_000364"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="U2M88_000364"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEM8372776.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="U2M88_000365"
     CDS             complement(2351..3031)
                     /locus_tag="U2M88_000365"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEM8372777.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="U2M88_000366"
     CDS             3157..4026
                     /locus_tag="U2M88_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM8372778.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="U2M88_000367"
     CDS             4052..4567
                     /locus_tag="U2M88_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372779.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="U2M88_000368"
     CDS             complement(4614..5207)
                     /locus_tag="U2M88_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8372780.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="U2M88_000369"
     CDS             complement(5232..5411)
                     /locus_tag="U2M88_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372781.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="U2M88_000370"
     CDS             complement(5488..5997)
                     /locus_tag="U2M88_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372782.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="U2M88_000371"
     CDS             complement(6093..6473)
                     /locus_tag="U2M88_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEM8372783.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6802..7551
                     /locus_tag="U2M88_000372"
     CDS             6802..7551
                     /locus_tag="U2M88_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEM8372784.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7735..9012)
                     /locus_tag="U2M88_000373"
     CDS             complement(7735..9012)
                     /locus_tag="U2M88_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372785.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9091..9735)
                     /gene="pcp"
                     /locus_tag="U2M88_000374"
     CDS             complement(9091..9735)
                     /gene="pcp"
                     /locus_tag="U2M88_000374"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEM8372786.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9934..10878)
                     /locus_tag="U2M88_000375"
     CDS             complement(9934..10878)
                     /locus_tag="U2M88_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEM8372787.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10880..11566)
                     /locus_tag="U2M88_000376"
     CDS             complement(10880..11566)
                     /locus_tag="U2M88_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEM8372788.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12040..12360
                     /locus_tag="U2M88_000377"
     CDS             12040..12360
                     /locus_tag="U2M88_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEM8372789.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12514..13419
                     /locus_tag="U2M88_000378"
     CDS             12514..13419
                     /locus_tag="U2M88_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8372790.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGVGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13416..14189
                     /locus_tag="U2M88_000379"
     CDS             13416..14189
                     /locus_tag="U2M88_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEM8372791.1"
                     /translation="MTLLLKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14370..15794)
                     /locus_tag="U2M88_000380"
     CDS             complement(14370..15794)
                     /locus_tag="U2M88_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8372792.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16005..16451
                     /gene="nrdI"
                     /locus_tag="U2M88_000381"
     CDS             16005..16451
                     /gene="nrdI"
                     /locus_tag="U2M88_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEM8372793.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16516..18498)
                     /locus_tag="U2M88_000382"
     CDS             complement(16516..18498)
                     /locus_tag="U2M88_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM8372794.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18512..19408)
                     /gene="murQ"
                     /locus_tag="U2M88_000383"
     CDS             complement(18512..19408)
                     /gene="murQ"
                     /locus_tag="U2M88_000383"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEM8372795.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19411..20472)
                     /locus_tag="U2M88_000384"
     CDS             complement(19411..20472)
                     /locus_tag="U2M88_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEM8372796.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20683..21249)
                     /locus_tag="U2M88_000385"
     CDS             complement(20683..21249)
                     /locus_tag="U2M88_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEM8372797.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(21277..22779)
                     /gene="eat(A)"
                     /locus_tag="U2M88_000386"
     CDS             complement(21277..22779)
                     /gene="eat(A)"
                     /locus_tag="U2M88_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEM8372798.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23037..24158)
                     /locus_tag="U2M88_000387"
     CDS             complement(23037..24158)
                     /locus_tag="U2M88_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEM8372799.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24284..25225
                     /locus_tag="U2M88_000388"
     CDS             24284..25225
                     /locus_tag="U2M88_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEM8372800.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25375..26271)
                     /locus_tag="U2M88_000389"
     CDS             complement(25375..26271)
                     /locus_tag="U2M88_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEM8372801.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26540..27880
                     /locus_tag="U2M88_000390"
     CDS             26540..27880
                     /locus_tag="U2M88_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8372802.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28098..28541
                     /locus_tag="U2M88_000391"
     CDS             28098..28541
                     /locus_tag="U2M88_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEM8372803.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29045..30463)
                     /gene="nhaC"
                     /locus_tag="U2M88_000392"
     CDS             complement(29045..30463)
                     /gene="nhaC"
                     /locus_tag="U2M88_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEM8372804.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30866..32089)
                     /locus_tag="U2M88_000393"
     CDS             complement(30866..32089)
                     /locus_tag="U2M88_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEM8372805.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32120..32959)
                     /locus_tag="U2M88_000394"
     CDS             complement(32120..32959)
                     /locus_tag="U2M88_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEM8372806.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32976..33656)
                     /locus_tag="U2M88_000395"
     CDS             complement(32976..33656)
                     /locus_tag="U2M88_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8372807.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33658..34731)
                     /locus_tag="U2M88_000396"
     CDS             complement(33658..34731)
                     /locus_tag="U2M88_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8372808.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(34787..35025)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(35068..35301)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(35378..36049)
                     /locus_tag="U2M88_000397"
     CDS             complement(35378..36049)
                     /locus_tag="U2M88_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEM8372809.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36136..38814)
                     /gene="mgtA"
                     /locus_tag="U2M88_000398"
     CDS             complement(36136..38814)
                     /gene="mgtA"
                     /locus_tag="U2M88_000398"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEM8372810.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            39473..39910
                     /gene="ndk"
                     /locus_tag="U2M88_000399"
     CDS             39473..39910
                     /gene="ndk"
                     /locus_tag="U2M88_000399"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEM8372811.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39932..40798)
                     /locus_tag="U2M88_000400"
     CDS             complement(39932..40798)
                     /locus_tag="U2M88_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEM8372812.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40958..43051)
                     /locus_tag="U2M88_000401"
     CDS             complement(40958..43051)
                     /locus_tag="U2M88_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEM8372813.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(43800..44423)
                     /locus_tag="U2M88_000402"
     CDS             complement(43800..44423)
                     /locus_tag="U2M88_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372814.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(44582..45979)
                     /locus_tag="U2M88_000403"
     CDS             complement(44582..45979)
                     /locus_tag="U2M88_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEM8372815.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(46011..46982)
                     /locus_tag="U2M88_000404"
     CDS             complement(46011..46982)
                     /locus_tag="U2M88_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8372816.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            47356..47781
                     /locus_tag="U2M88_000405"
     CDS             47356..47781
                     /locus_tag="U2M88_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372817.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(48269..48970)
                     /locus_tag="U2M88_000406"
     CDS             complement(48269..48970)
                     /locus_tag="U2M88_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEM8372818.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(48986..49426)
                     /locus_tag="U2M88_000407"
     CDS             complement(48986..49426)
                     /locus_tag="U2M88_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEM8372819.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(49426..50001)
                     /locus_tag="U2M88_000408"
     CDS             complement(49426..50001)
                     /locus_tag="U2M88_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEM8372820.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(50849..51328)
                     /locus_tag="U2M88_000409"
                     /pseudo
     CDS             complement(50849..51328)
                     /locus_tag="U2M88_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(51487..52188)
                     /locus_tag="U2M88_000410"
     CDS             complement(51487..52188)
                     /locus_tag="U2M88_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM8372821.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(52296..53705)
                     /locus_tag="U2M88_000411"
     CDS             complement(52296..53705)
                     /locus_tag="U2M88_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEM8372822.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(53753..55618)
                     /locus_tag="U2M88_000412"
     CDS             complement(53753..55618)
                     /locus_tag="U2M88_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM8372823.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(55739..56590)
                     /locus_tag="U2M88_000413"
     CDS             complement(55739..56590)
                     /locus_tag="U2M88_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEM8372824.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(56900..57613)
                     /locus_tag="U2M88_000414"
     CDS             complement(56900..57613)
                     /locus_tag="U2M88_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEM8372825.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(57681..58007)
                     /locus_tag="U2M88_000415"
     CDS             complement(57681..58007)
                     /locus_tag="U2M88_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEM8372826.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELVDVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(58130..58357)
                     /locus_tag="U2M88_000416"
     CDS             complement(58130..58357)
                     /locus_tag="U2M88_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEM8372827.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(58679..59932)
                     /locus_tag="U2M88_000417"
     CDS             complement(58679..59932)
                     /locus_tag="U2M88_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEM8372828.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            59993..60733
                     /locus_tag="U2M88_000418"
     CDS             59993..60733
                     /locus_tag="U2M88_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8372829.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(60819..61577)
                     /locus_tag="U2M88_000419"
     CDS             complement(60819..61577)
                     /locus_tag="U2M88_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEM8372830.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(61574..62602)
                     /locus_tag="U2M88_000420"
                     /pseudo
     CDS             complement(61574..62602)
                     /locus_tag="U2M88_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(62622..65789)
                     /locus_tag="U2M88_000421"
     CDS             complement(62622..65789)
                     /locus_tag="U2M88_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372831.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(66013..66897)
                     /locus_tag="U2M88_000422"
     CDS             complement(66013..66897)
                     /locus_tag="U2M88_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEM8372832.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(66992..67429)
                     /locus_tag="U2M88_000423"
     CDS             complement(66992..67429)
                     /locus_tag="U2M88_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372833.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT        19408 a        14217 c        11665 g        22267 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aacagaaagt
     6601 aggtcctcca agacactctt cctgttccca atataataaa tgaaagtaag aaattatatt
     6661 ttcaagaaaa caaacagaaa attatcataa cacaaattat ttataattga aagactagtt
     6721 ttttttcaag aaaagcatac aatagattgt gttagtaaat acagaacaca ctacatttag
     6781 tgtcttaagg agtcctcata catgaagtca tattttcagt ttttagctca tcagctaaaa
     6841 ggctggccgc aacaaaatta ttatcttttc tttttcagtc ttggctgcca ggtcatgact
     6901 cttgtcaata gtcccatcac ttggctgtcc gttattacat ttatcggtac cacgcttggt
     6961 gtattgtgta ttttatccat caatgcggca aaatcagtca acggtatttt aggtattttg
     7021 tctgcgattt gttttattat cgttgggttt tcagcaaaga actatttaag tatcggtgaa
     7081 cagattgctt atatgatcac attggatatc cccgtattgt taagtaaaga atggaatcat
     7141 aatatggctt caaaaatccg aaaattcact ggaaaaagct ggtttatcgc aatcatcagt
     7201 actttccttg tctatcttgt ttcaggattc ctgattggtc gttatacaga tgatcctcgg
     7261 ccgtgggtag atgccattgc tttttctatt agcttgactg ctggtatcat cagtttcttg
     7321 cgttacaaca accaatatta ctggtggtta gcttcagggc ttgctcaaat ggtcctttgg
     7381 tatatttcat ttacgcatgg atctgcaagt ttggcgatgt tcgtgaacag ctctgtttac
     7441 ttgctgaatg atgtccttgc atttaccgtt tcaccttggt ataaccaaaa agaacgtgaa
     7501 cgtatgcaaa aaatggaaga agaatactac aacgcacaac aacacgcata aaaaaactga
     7561 gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga
     7621 atcagaagca actccttcgg aaataagctg aaattcacaa aaatttgaaa agcaattttc
     7681 gtgaatttct tcttatttct tggagttaaa cgcttctgtc ccagcctcta ttttttatag
     7741 taattccttc agatactttc ttgcctcatc attgaaacgc agtgattctt ctaacttctg
     7801 ataatattcc aacgcttgtt tctgagcttc agtaaaacga taatgttttc ctttccattt
     7861 gtcgattgtc tcttgcacat ttccagattg gatatctttc actactagtt gccatttttc
     7921 ttttgagact gtacggtctt tcttatcagc agattgtata gcttctcgtt ctttacgcaa
     7981 tacttttaaa aaaccatttc tgactatccg aggaatgctg tcgaagaacc cttctgtaaa
     8041 accatagcga caatattgct tccaaataaa tagatagatt tttggttgga tgaatcgttg
     8101 gtttttcgca aaatattcca tctgagcagt gacttctggt gttcgttgac gttttgtctt
     8161 tctcaacacg gaaataaatg tttcaaaata agcattagga tgtactggca cttcttcata
     8221 tacgatggat tgccaatctt tacgtaaaaa tgcgcgatag ttgatcaaga attcttctac
     8281 atcctttact aattcttctg caaagtcgtc aaatagttct ttttttatct gtcgcttttt
     8341 tgactgccca ttgatgtgtt cacttatttt cttgatttca ttttccaatg cttgataatc
     8401 cgctatgtaa atcggcattt ctacttggcg aaattctgcg agccgctgag ctaattttat
     8461 atccggtaaa tatggctgtt tcatccgacg attttggtac aagacaaaac agtatttttg
     8521 ccatagtcct tcaggaaaat cgatattctt ctgcttaagc cataataatt cgtctaaggc
     8581 cagatcaact tttgtcattc cctgatggat cgaagctgcc actgtcggag agacatgcag
     8641 gtgatcggca aagtggttga tgcctagttt taggatcttt ccggtttttg gcgatttaac
     8701 gatgtattgc acttttaatt ctctaccaca ctcacaatac aatttttgct ggcttcgttt
     8761 tgttcgaaaa aacggattga tcttgaagtc agaaaacacc caatctgttg cagctaaata
     8821 atgttcttcc ataaaacgag agctgattag atatcggcgg tgcttatcga ctagttcccg
     8881 ctgttttttc gttaattttg catagatctc ccaacgttga ctgcttgtgt attgtttaga
     8941 aattttgatg ggatagcttt tgctgatttc ttttggaaac cattcttcaa agacggattg
     9001 ccattcttcc acggctgttt ctttcctttc taattacaaa aagctggcta ctaacataac
     9061 gttagcaacc agccagtgtt tcttgctaaa ttagtgagtt gttccggcat ttccggaaat
     9121 atcttcttta ttttctactg ctgcttcaac tgcataagtc aaagaagaag caatgtcttc
     9181 taaagacata gaaggcgtac ccactggacg atcgatcact tgatcgggtg agaatggcac
     9241 gtggatgaac ccacctcgaa ttgccgggaa ttgtgtttcg atcaaatgaa gcaaacgata
     9301 catgatatca ttgcagacaa atgttcctgc tgtataagag atgtacgctg gtagaccatg
     9361 atcttgtaca ttttttgtca ttgctttgat tggcaatgag gtaaagtaag cggctggacc
     9421 gttttcttct aaagccgtat cgaatggctg atttccttca ttatcaggga ttcttgcttc
     9481 tgctagattg attgcgacac gttcgaacga gactgctgac cgcccacctg cttgtccgac
     9541 acagatcacg acatctggtt ggtgctgttg gatggcttct ttgactactt gactgctttt
     9601 ttcgaatacg gtcggtactt ccactttgat gatttcagca ccgctgattt catctggcat
     9661 tttttttact gcttcatagg ctggattcac tttgtcccca ccaaatggat caaaacctgt
     9721 tactagtact ttcatctgtt gttcctccgt ccgattgatt taacatatgg ttatctttta
     9781 tcataccatt ttccttgaac agcgtaaata aaggaaacaa tagtttaaat aacataacag
     9841 atcaaactta atatattctt ataaaaaaat agtgttccac tacaatgtta gaagaggttg
     9901 cgacaattat tctgtcacaa cccctctctt ctattattta aatagaatca tataagcaat
     9961 ttggaaaact agcataaata acgcaataaa caactggttt ttgatcactc cgtatttgtc
    10021 tttcatctcc agcatcgcta caggcacgat attgaagtta gcagccatcg gtgtcagcag
    10081 ggtcccacaa aaaccgcaag tcaatgcaac cattcctatt aacgctggat tcgctccatg
    10141 actaaatacg aatggtgcac cgatcccaac cgtcatcacg gtgatggccg caaatgcatt
    10201 ccccatgatt accgtaaata tcaccatgcc aagagcgtaa atgatgatgc cgatgttcac
    10261 atttccttct ggaacgatcg tacctactgc attggaaatc actgtcccaa ctccagcgct
    10321 tgtaaatacc gcacctagag aagctaaaag cattggcaac atgcttaacg gccctactgt
    10381 gcccagcata tctgctgcat catctaaaaa tgtacttggt cgattatctc gagaaaagaa
    10441 catcaagatt agcatggctg caaaaacacc gacgcccacg ccgactaaag cacctaaatt
    10501 ggtaaataga gcaaacaaga tcgcaaagac accgatactc aaagctggaa taaaaatttt
    10561 cattcctagt ttgtctgaca ttttttccat atattctttt gatggaagcc ggctttctcc
    10621 tttgctgact ttcttagcaa ttgctggtat ggtcatcgca aagatcaagc caccgttcac
    10681 gattgccgga agccatcttc ctcctgcaat aactacccct aagaccgtcc agaaaaaggc
    10741 tgacccataa cgatgaggat tggttttatc tgttaaattt cttactccag tgtaaatcaa
    10801 tacgagtccc atcacgatat aaaggatctc cagtaattta tcgcctaata aaatatcagg
    10861 atttgtaaaa aagtccatct tattgcccct ttcctggata atatttcttc gctaatttct
    10921 tttctttaag atagaagtag acacttgcca caactaaagc cgtgatagcc actggaactt
    10981 ccactaaagc aagctgtccc aatgttacgt catatcctag atctttcagt gttgattgga
    11041 ctagcaacgc ccctgctcct ccgatgaata gtacttggcc aaagaaccag cagacatttt
    11101 ccatagctga agccatacct tttagttctt cttcatgctt aggatggact tctaaccctt
    11161 ttgattcgat ggtccccaat gccatcggta aaatgattgg acgaacaaat ccagcaactc
    11221 caccaaaact cacgttaaaa gccgcaaaga tcattcgaaa gatcccataa atatctacaa
    11281 tcataccagc agatactttc ttgaatcgct tgatcaagga agccgctgat tctttcagac
    11341 catttctttc aagcgttcct gttgccaaca taatgatgat aaaaatcgcc atcccgcgat
    11401 tgtttacaaa acttgtacct aaaagttcta acattccttg aatgccaaga ccgccagtca
    11461 aagctgtaac aactaaagca accattacga tcaatatcga gtctaattta aatgcaaacc
    11521 cgagaataac aatcaagacg cctaaaaggc taagataatt ttccatatat tttcctccat
    11581 aaaatgacaa tattttttta attataaagc aaaacacata aaaaatatag ttgttttctc
    11641 acgaaataga cactattttt aaatattttt cgttattttt ttatcacaaa aattatctta
    11701 cttattgtaa ggtaaagctt tttttaattt attgcccgat aatatataga aataaggtct
    11761 agttactgtt ttttttaaat gattaataaa taacaacttc tttctatgaa attgcaaagc
    11821 attcatcttc ttttccgcta acgatatatc cgtcagtgct tggtacactt tactaaaaag
    11881 tatgagattg ccgatatctg gataggtgtg ctatactaca gaaaaagata tacaatctgc
    11941 caccgggtag gataaagagg atgatcattc cattaggtct ttgaagggat gattttcctc
    12001 tttttgtgta tttttttata gttaaggagg aatgtaagta tggcatggat ggaattaatc
    12061 atagcaggaa ttttggaagt attctggtca acgatgatga aatggagtga tggtttttct
    12121 aaaatcaatt attcttttta cacagtgatt ggcatgattg ctagttttta ctttttgtca
    12181 aaggcaatca aaagtttgcc gatgagtctc tcttatccta tctggacagg gattggcgca
    12241 gttggttcgg tcataattgg tgtggtcttg tttcatgata agctgaatct ttccacttgg
    12301 ttctttgtgg ggatgctact catcagtatc attggcatca agattactag cggccactag
    12361 acacttggaa taagtactga tcagaaaaaa taagcctttt tatttaatat tcaatttttt
    12421 tatgaaaaaa taagaaataa gtccaatgac ctagcgactt ctccactcat caggtgtatg
    12481 cttgtactat ctatagaaaa ggagtgaata tttttgaaaa gtctttcggt ccaagaatta
    12541 tccaagaact ttggtcacaa acaagccctc agcgatgtca gttttagctt atcccctggc
    12601 aaaatcgttg gattagtagg tcctaatggc gttggaaaaa caacgatcat gaaagctatt
    12661 ttaggactga tccattactc agatggaaca atctgtattg gtgaaaatga gatttctccc
    12721 tctagtcata aagggttgga acaagtagga gcattgatcg aatacccggg tatctatcca
    12781 ttcttaacag gctatgatca tctgaagtta ttctctgaaa ccaacgatgt atctgctatc
    12841 gacactatcg tgaatcagct caagatggaa aagtacattc acaaaaaagc gaaatcctac
    12901 tcgttgggaa tgaaacaaaa attgggaatt gctcttgcct tactgaacca tccgtctatc
    12961 gttattttag atgagccgat gaacggtttg gatccacaag caacaagaga tgtgcgaaaa
    13021 atcattaccg acttggctac tcagggcacc tctttcttga tctccagtca cttactcagc
    13081 gagcttgaaa aaatggtcga tgagctgatc ctgatcaatc aaggaaagat catcaaacaa
    13141 tgtacaatgg ctgaattgaa ccaaacagcg caagatttca tcgttatctc tacaacaaat
    13201 gatctagctg ctcgaacgat cttgacagaa gctggatata ctttggaaga tcctgataaa
    13261 atcaagcttt taaaagaaaa cgaagaatta ttaggaaccg tgatccagct attgtctgca
    13321 caaaatatcc atgtgacaga cgtccagcat ctgacgaatg atttggaatc ttctctattg
    13381 gaaattctgg attcagtaaa gggggaagaa gaaaaatgac attgctttta aaacaagaat
    13441 tatttaaatt gatcaagaaa aagtcaactg ctgttttatc tgttctatta gttgttttgc
    13501 tcataggaac agcacttcta gccaaaaaat acacgacgat cattgatcca gttgaaatga
    13561 ctgctcagct ttttagtgcc acatcttgga ttgtctttat catgattgca gctgcaagca
    13621 cgatcatttc gatggaagca caatatggta cattgaaaaa tctcctttat cgaaagtatt
    13681 ctcgaggaga aatattagta agcaaatgga tcacattagt tatttattct gtttatttat
    13741 accttttagc tattattgtc actgtgttga tgaaactgat tcttttccct tcgatttcct
    13801 tcacggagaa ggtttctact ggtcaaacac tgatccaaag cttgttcact tatactttgg
    13861 gtagttatat cggtttatgg ctgattttaa gtttagtgtt gatgattgct tgctttatca
    13921 atagttccgg cgcatccatc tcagcaggaa tcgtctttta ttttgcttcg tcaatcatat
    13981 ctggtatcct tattgcactc atccaaaaat gggagtggat caaatggaat ccgatcagta
    14041 tgttgaatct gcaaaaccaa attggaaacg aagagatcat gaagcaattg actcatcttt
    14101 ctactaatca aatgctattc ggtaatttag cttacatcgt tttgttctta gcattaggat
    14161 atctcgtatt taagcgaaaa aatgtatgat gactcatcaa aaaagaggct gtgaaagaga
    14221 cgttcagcct tattgccgat tggctgtctc ttaaacaccg tttattctga aaaaggagct
    14281 gtgacaacat ttgtgtcaca gctcctttcc tagtatgttt ttgaataaac gacaggctca
    14341 caacctctct ttatacttct tttatacttc tatacgtgaa gcatcccagt ggcaaataaa
    14401 accattgcaa tcactccgac catcgtcaca cctataagta aaatcttatc taagtggata
    14461 tgttgtacag gctcgttttg ctccttacgt gctttgaggt aaaaataaat gcctggcaag
    14521 aaaagaacaa gaacaagcat tacttcatta aaggctacga ctagcatagc ccatacttga
    14581 aaggcacttg ctaaaagccc tactaaaaat tggacccatt ctttccgctg ataagaaagt
    14641 ttcatttggt acaatcctaa gaaaagccac gtgaagaaaa tagctgctgt accaagtgtg
    14701 taagcaaagt tataagcact gtcagtgaat aataatgaaa gtaaaaagac attcgtacat
    14761 aacgtcgtca atacgatagc ataagttggt gccccatttt tgttgagttt tccccatgct
    14821 ttaggcaaaa gtccgtcttc agccatgttt cccattgttt caatcggtaa gatcgtccat
    14881 gagagccaaa caccaactaa agaaatgatc aaaccgccgt tgatcacgac tgctccccat
    14941 ttaccaacca tcatttcaaa cacatagccc atagcaggct gcgccttgat cgtttgcagc
    15001 tgattcatcg tcattgtccc atatggaagc atggaaacca agacgtaaat aataatcaag
    15061 ccgaatatcc ctagaatcgt tgctttccca acatctgatt gtttttttgc tcgtgaacct
    15121 acgacagagg ctccttcaat accaacaaat acccaaagca tcaccatgat gcaactcaat
    15181 acttgactgg aaaacgggat cggctgtcca ttcgcattca atataatgtc gctagaaaag
    15241 ttatcaatga attgttggat cgaaaacgtg ctgatagcta ctacaataaa taaaaataat
    15301 ggcactaatt tacagaatgt gacgatagca ttgacgaaac tagcattttc gactccccga
    15361 ttcactagcc atgcatatcc ccataaaaag actgtactca tcacgatcga tgccagattt
    15421 tgcccacctt caaatactgg gaagaaatat cctaatgcgc tcatcaacaa tgttgcaaaa
    15481 gctacgttgc ctaaccagca tgtgagccag taaccccaac cgctgataaa accagcgaaa
    15541 cggccaaaac cttcttctgc gtaactaaag atcccgcctt ttaaatctgg acgtttttta
    15601 ttgatattat tgatcgacaa ggagagcatc aacattgctg ttcctacgag tacccaactg
    15661 aaaatagctg ggccgccagc cgatccggag atgtccgtca taataccaaa tacgccacct
    15721 ccaatcgctg aaccaattac taaggcagca agtggaatta aaccaattcc ttttttctgt
    15781 tgctctgtat ccatgtaagc atcctccaaa ataaaaccgc ttttatcaaa aagtataaca
    15841 aacctttttt atttttactt aattaataaa aactttaata gaaatataat tctaccggct
    15901 tttttctcat tatacaagca aagcgcctag tccctttcca aattacacca ccaaaaaact
    15961 taaaaaagat gctatactaa aaggaaagaa ttggaggatg atccatgaac attttatata
    16021 tatcgatttc aggaaatacg cgtgcctttg ccaaacatct agcagaatat gctgaaaaaa
    16081 tgcatgcaga agatcctgtc aatcctgaag ttactttaaa agaaatccat gaaaatagtg
    16141 attttacgaa agaaacagaa ccatttttta ctttcgttcc tacttattta gacggcggaa
    16201 atggtttaga taacggtgat acagaaatct tgaccgaaac gatgcgtgaa tatctggaac
    16261 atgaagacaa tcataaactt tgtcttggtg tagtcggtag cggaaataaa aacttcaata
    16321 atcagtattg tctaacagcc aaacaatatg cacaaacatt tggctttcct ttcttggcag
    16381 attatgaatt acggggaaca ccatctgatg tgacacgaat ttatcagatt ttagcagaaa
    16441 acaataaata ataaaaaaga agaagctgaa aaatagattt cagtttcttc tttcgtttct
    16501 attgtttcta tcatattaag ctttgacatc catcacaggt gcttttgctt ttgctttacc
    16561 tgttttttcc aagacaaagt tttttacacg atccatattg gttaccaaga cgatcatgga
    16621 tgtttctttg cctgctgctt tgatcgcttc taagtcagct tctgctacaa gcgtatcagg
    16681 tgtcacttgc tgcccttctg ttactttcac atcaaatggc gtaccgccta agtcaactgt
    16741 gttgattccc atatgaagca agatttctaa accatttggc gttgtgatcc caatagcgtg
    16801 tttcgttggg aagacagagc tgactacccc agtaactgga gaatagatat gtccgttttc
    16861 tggttctaca aagtagcctt cacccatcat cttttgtgca aaaactggat cacttgcttt
    16921 atctaaagaa acaactgttc cgtcagcaac tgatgagagg atagtcacac tttcagctga
    16981 agtttgaggt actacttttt caggcacttc atttactgtg gctgtttcag tcgctaattc
    17041 ttctttttct aaacggtctt taggaatacc gaagaagaat gtagcaacga agccaccagc
    17101 ataagctgcc aataatccaa ggatgtagcc taaccattta ccgtcagaga tcaatgggat
    17161 caaagcggct cctgaaggtc caatagcgat agccccgaca tttccgatca ttccgatcac
    17221 tgccccgccg atacctccac cgatacaagc agtaataaat gggcgtccta gaggtaatgt
    17281 tactccgtaa atcaatggtt cgccgattcc taggatcccg actggtaaag ctcctttgat
    17341 catttctgtt aattctttat cttttttgca gcgaatccat aatgccaatg ctgcacccac
    17401 ttgtcctgca ccagccatcg caagaattgg aagtaatagc gtacgaccag tttcatcaat
    17461 catttgcaca tggattggag tcaaaatttg atgcagtccg aacataacca tcggcaagaa
    17521 taatgtgcct agaacgaagc cggcaaatgc tccaccaacg ccaagtaccc agttgatccc
    17581 accgaccatg ccatcagaaa tgaaacctgc taaaggcatg atgaagaaaa tttcaatcag
    17641 accgatggca atcaatgaaa gagttggtgt aacgatgata tctaccgcat caggtactac
    17701 tttatgcagt tttttctcaa cgattgacaa tagccaaaca gcgaagatga cacctaggat
    17761 cccaccttgt ccagctgcaa gagcttcacc agtaaataag tttttcagtg gtgcttccgg
    17821 attcatccct gtcagcatca caacggcccc gataactcca ccaagagttg gtgttgcacc
    17881 aaaaacttgt gcagcattga tacctgtata gataactagg tagctaaaca tcccgttttt
    17941 caagatgttc atcacatcga tatattgttg ccatgttgct gcatcaagtg ttccggctgt
    18001 gactaagttt gctaaaactg cggcaatccc tgccacgatc ccagtaccga caaatgcagg
    18061 aatcattggc acaaagatat tagaaatgtc ttttaatacc gatttgattt tagatggttt
    18121 ttgtttcgat ttttgtgccg ctttcatttc ttgtgcccgt tcgtttacac ggtcttttcc
    18181 agatgctgat gaatttgctg tagctgtact tacagaagaa agtttttctc ctaatttcac
    18241 gcctgccaaa tcaaccattt gctgtgctac tttgttcact ttcccaggtc cgatgatgac
    18301 ttgaagctgt tcatcttcta ctacaccaag cactcctgga atcgctttta attcagagac
    18361 atttacaaga tcatcattga tgatcttcat acgcacacga gtcatgcagt ggacaatgct
    18421 ttctacattg cccatgccgc ctgccccttt ataaatttgt tttgccagtt cttccaaagt
    18481 cagttcgttt tttgccatgg tcttttcccc ctcataaggt cccttttaca aatccgtttg
    18541 cttcagctaa ttttgcttga gcttcctctt tatcactatt tgtcaaaatc atgacgatag
    18601 ccagcttaac attttgatct gcttcttcaa agtacttttc agctgtttcg taatcagctt
    18661 ctgtcgcttg catgatgatt cgttttgagc gttcgattag tttttcattt gttggtttga
    18721 catcgaccat caagttattg aatactttac ctataccaat catactgata gtagaaatca
    18781 tattcaagat aagtttttgt gctgttcctg acttcaatcg agtagagcct gtcaaaaatt
    18841 ctggacctgc atcgacttcg attgggaaat ccgcgtgttt actgatttcg gcttgtttat
    18901 tgcaagaaat cgttcctgtg acagcaccga tcgattttgc gtaatctaat ccaccgatca
    18961 catatggtgt acgaccgcta gctgcgatac caataactat atcatttttt gtcaaattca
    19021 gcttgattag atcttcttgc cctagctcat aagaatcttc ggccccttcg acagctacag
    19081 tcattgcttc ttctccacca gcaattagac ctatgaccat ttccggttct acaccgaatg
    19141 tcggtacgca ttcagcagca tctaacacac ctagtcttcc acttgttccg gctcccatat
    19201 aaatcagacg tccacctttt ttaaaggact caatcgtgtt agcaatcact ggttcgatac
    19261 ttgtcagttc tttttcaacc gcagcggcca ctttctgatc ttctttgttc attagttgaa
    19321 cggcttgaga aatactcatc tcatctaatc caaaagtttc ttggtttctg cgttccgttg
    19381 ttaggttctc taaattgatt ttgctcattt ttatcttcct tcttttctta taaattgata
    19441 gttcatacca gctttgattt ggttgatcaa tggtagctct tctttgataa ctttagctac
    19501 tacattcact ttttcatccg ctggcaaatc atatttggtt aattggatct cacccatata
    19561 gcgcaaatac ttttcgttat ctaaagtaat agaccctttc atacgcgtag ctgtatttct
    19621 tgctgggata ggcggaatct ctcgaaaacg tgcatctacg cttcgtatga catctcttgc
    19681 atcgtcttgc cgattcgtat gcttccctaa tatatattcg taaaattctt catctacagg
    19741 ttccgtatgg agcaagactg tctgttcttt ttggaaagaa gcaaattgct cctgaacgtt
    19801 ttctgacaga cctgcatctc cgatgtaaac aatatccgtg ttacttgcta atagatctag
    19861 agcgcctgca agaggatgga tccctcgatg ttcttccaaa gttggcaatc cttgatatag
    19921 tgggcctctc aactctgtat ctcctggtac aaaaccttgg acaaggagac cttgaatttt
    19981 gagccattga ttttttttct gataccattc tttgtccagc cctgtttcgg gacgtggata
    20041 ataattatgc caagcttcaa tctgtgagaa atcagcttct gcttctttca attcatcgat
    20101 atctttaggc gtgatcgtgc tagcattcaa actgattttc aattgatgtg accagtcggc
    20161 aatctgctga ttggaaatat gataatccat tcttaaacct gttacaccaa ttgctttcaa
    20221 aggcgttaga tcttctaatg aaaaacccgc tcgttccaat gcctctcctg aaatatcgac
    20281 catcaattct agtttattct ttttcgcttc gcttcctagt tcgatcaatc gcttttgata
    20341 tgccgaaaca tcatcttctg gtatatgcat ggaagtgaaa ataccgacaa agccattgga
    20401 tgccatttgg cggatatact gtttcttctc ttctgacaaa gaatcgttca taaatattga
    20461 aaagccaaac atattatccc tctttttctg tatccgtctt catttctatg actagagtat
    20521 atatctaaag aaacaaaatt tcaatacttt tattaaaata attaaatttc atttctaaag
    20581 gttgagttta tattatcaaa agaattgtca taacctaaaa aaggcactgc taagcaaaag
    20641 cagtgccttg aaatatttta tttaaataac acaaaaacat aattactcat aagaaaccaa
    20701 ttctgtatta tctaccatga atgtaaagaa cccctcatta ggcatcggat gaaaataagc
    20761 attgatcact cttatcaagg cattatggca aactaacaga tagacatttt cttcgtcttc
    20821 gatgcattct ttcaataaag gaacgatccg tgcatacaca tctaagactg actccccatt
    20881 tgggaatcgt acagcgaacg ctaaacgtgc tttttgaaag ttctcatctt tgcttggcag
    20941 tccatcataa tccccaaaat ccatttctac caatcgttca tcaacggtca gcggcaagct
    21001 gagacgatca gcaactgctt gtgctgtatc tcttgctcgt tgaagagggg aatggatgat
    21061 tttcgtgatt ggaacaggca aatctactac cttttctgct agtttctgtg cctgcatctg
    21121 tcctttttca gtcagcggca catctgcccg tccgcaaact aatccttgtg cgttccaagt
    21181 tgtttctccg tgtctcgtta cataaagcat gtgattctct ccttcgttgg caaagttccc
    21241 cttaaccata ccttgttgtt cttgagatta caagggttag attctctcaa gagaaataat
    21301 ctcagtagat actttatcca ggaaggtttg atcatgttcc actagtaaca tggcaggttt
    21361 cacgttcaag atcagttgtt ctaattgttc ttgattgaag acatccaaat aattcaatgg
    21421 ttcatcccat gtatatagtt cagctggctg tgataaagat ttagccaatt ccactttttt
    21481 ccgttggccc atactcatct gctcgatctt gttatgaaaa acatctcttt ccatcccaag
    21541 ctttcggagg ttgttcaaaa atgcttggta gtctacttgg tttttctctg caaattccgc
    21601 caacgttcct cgattgtctt cataattttg gcttgcataa ctaatgctca gatgttttgg
    21661 ctggtatttt tctcctataa ccttgccgtt gaatgccccc agaagataat ggataatgga
    21721 tgactttcct gcaccgttag gacctgaaat cgccacccgc tgatgaggct cgattgtaaa
    21781 atgaattggc tcgaataaca gattttcata ccctaattga agatcttcta cgcttaaaag
    21841 tcgcttatgg tgagacgctt ggctattcat cgtcaaagaa tcgatatatt cgatatcttt
    21901 taatagtttt tctttctcgc tgatctgggt ctccatccga ttcacgattg ctttggatcg
    21961 tttcatcgtc cgtgcagcat cagcacccac tgctccttta ttcactcgtc tagattcagt
    22021 atcgatgaat ccgacttgtt tctttgtttt atctccttct cgggaacgag accattcggc
    22081 tttttcagct gctgttttct ttagcctact gacttctttt ttcaattttt cattttgagc
    22141 catttcaaat tcatcacgaa gttttttctg ttcttcatag atagagaaat tcccttgata
    22201 aagttccagt tgactttttt cgattgctaa aacatggtcc actacttcat cgataaatcc
    22261 ccggtcatgg ctgaccacga tgaagccttg ttttttcttt ttcaaataag ccgctacttg
    22321 ttttctacca gagatatcca aatgattcgt tggttcatcg attaacggga aatgagtgtc
    22381 atccacaaat aaaagtgcca gtaggacttt tgtcttctct ccccccgata gtgtgctgaa
    22441 ttctctccat aagatttcag gatctgtttg catcaattgg agctctcttt cgatttccca
    22501 tatctcaaaa tccgtaatat catttaacac gtaataggtt aaacgttctt tatcttttgt
    22561 ctgttgcggg aaataggcaa attcttgctg atggattact tgcccttgat aaggtagttt
    22621 gttttgtaga atattcagta aagttgtctt tcctcgaccg tttcgtccga taagtcctag
    22681 tttccattgt gtgtcaaaat ttagatttgc ttgttcaaat aataatgtgc cttggctgtc
    22741 gtagccgaat gtcagatttt ttatttcgat tttagacatt ttccatcacc tcgtaaaaaa
    22801 agaccagtcc tattttcaga actggctaaa gaggatgcaa gggttggata cacacaaaaa
    22861 gacccataca tcgaaaaaac caagccaatc ccgaaaatct gcacactgct tccttgtctc
    22921 cacaaggtac agtctgaatg cagctttcat caatcgactt aatttttcaa tgcacggatc
    22981 cctcactttg tcctatattt gaaattacga tagcataaag aaaatgattt tgtcaactac
    23041 ttcgggagga tggtgaaaaa tctgctaagt gtctgtccta agatttttgt tttttctaat
    23101 gtttgcctgt gttccattgc ttcttctaaa cctattggac ccaaatgaat acttaagaca
    23161 attgggagaa aatcagaaag tccttctagc tcttggtctc tacttccgca aatagcgatt
    23221 attggttttc gatatttttt tgcaagcact gctacaccgt atggaacttt tccgtggatc
    23281 gtttgctgat cgatccgccc ttctcctgta atgatgacat ctgcttgttc gatctttttt
    23341 tccaatccag tgatttggct gactaaatcg aaaccagaaa ccatcttcgc tcccaacaaa
    23401 aaaagggctc caccaatacc gcctgctgct ccacttcctg gaatatcatc taaatccacc
    23461 tcccagaaat cttttatttg ctgagcaatc tttcctgctt gtttatctaa gaaagctagc
    23521 gtcgtatcta ttcctccttt ttgtcttcca aattgccaag cagctccttg ttgacctgta
    23581 tatggattgg taacatctgc agcgataatc aattttactt tagaaacaag aacgtttctt
    23641 cgatcaaaat gaagtttttt agtagataat aaaggatttc cttctgtgta cccttctaaa
    23701 gaagcaccca aggattggag taatcctaga ccaccatcgc tacacccgct tcctcctaaa
    23761 gtgacaataa tttgttctac ttcttgaagc agtgcatctt taataagttc tcctaaggca
    23821 taactggaag caagtcggat agtttgatcc gttggtttat caagtagatc taaccccaat
    23881 gcttgtgcag attcgatgac tgcggtgttt tttccttgcc aagttgtcaa taaatagctg
    23941 atttttcgct gacggaataa gagatccatc ccatcaatgg tacgccatgt tcccctgagc
    24001 gcgtaataga ttgcttctgc agtattttct ccgccatctg caatcggcac attgattttt
    24061 cttcctttac gatcttccca gccatttaga actgcctgat ttagttcaaa tgaagaagca
    24121 cttcctttaa atgaatcaat tgccgccaaa atatccatct gtatccctca ctttcttctt
    24181 aagtaagaca ctttttttca tttttttcaa acaaaacaat gctattttct tccatttttc
    24241 tatatactaa aagggaactc attgaataaa ggagagaaaa aacatgaaaa tcgcaatagc
    24301 tggggccgga gcaatgggca gccgaatcgg attgatgcta catcaaagcg gaaatgaggt
    24361 cctcttgatc gatcgttggc ctgctcacat tgaagccatt cgtacaaatg ggttgattgc
    24421 tgactttaac ggtaaagaag tggtcgcaaa actgccaatc tattcaccag aagaaatcat
    24481 cgaaagcaac gaacacgtgg acttgatcgt agcattaaca aaagccaatc aattagatga
    24541 tatgttttgt tcgatccaat ccattattac tgacaatact tatgttttgt gtttactaaa
    24601 tggattaggt catgaagatg tactggaaaa atatgtgcca aagaaaaata tcttatttgg
    24661 gatcacgatg tggacagctg gtctagctgg tcccggaaaa gtcacattgt taggtgatgg
    24721 agaaatcgaa ctagagaact tggaaccaga aggcgaagca tttacaaaaa aagttgtaga
    24781 agtctttcaa gaagcaaatt taaatccgat ctatagccat aacgttcgtt attccatttg
    24841 gcgaaaagct tgtgtaaatg gaacattgaa tggtttatgc acgattttgg attgtaatat
    24901 tgctgaacta ggcgcgcaaa aagcggcaga gagcatggtt cgaacaatcg tatctgaatt
    24961 tgcttctatc gcagcaaaag aaggaatcat actagatcaa gaagaagtgt accaacatat
    25021 tgcttctact tatgatcctg acaacatcgg gttgcattac ccatctatgt accaagacct
    25081 gatcaaaaac catcgtttga ctgaaatcga ctacatcaat ggtgccattt ggcgaaaagg
    25141 acaaaaatat gatattgcta caccatattg tgcctttttg acccaattag tccatgcaaa
    25201 agaaggaatc cttggcgcag aataaaaggc tctctgatga gaatcagctg aatcagcagc
    25261 tggttctctt tttttaagcc caataaaaga tcaaaaagaa aacacaactc tcatgatgcc
    25321 ataagaattg tgcttgtttg tttcagtaca taatctcttc aaaatgtagg ttttttattt
    25381 gttttcaaca gaggagaggt catattcgta ttgctcataa atagtggaat cgttagtgat
    25441 aaatcgtttt gcccaatttt ctgcatcata aaattccaat agctctgata ggattggctt
    25501 aaatggtgta tgcagaaatt ccggtaattc atctaaatca cgttctcgaa gcaaaaaaat
    25561 attttcagga cgataaaaat gataatgagc aatcgcttga ttatccgtca ctagtttctt
    25621 ttgaaaatac atggattcaa atgtccgcaa agtcactcct tgctgaccct cccgcaacag
    25681 ttccagaatc ccgcgcgatt cccctgtttt tttcaggtac tcttcataag taagatatcc
    25741 aggttgttca ttccctcgat gtggaagaat gaaatagttg gtcgtaacat ctctttttat
    25801 aaattcctgc ttatagcttt ctgctcgttc ttttctccca ttattcgttg caccaaaaaa
    25861 tagatccgtc tttggttcgc tagaagaaaa attcatatgt ttcgaataaa atgaactatt
    25921 atgtttcagc ccaagttctt ttgcttccaa aaaatcaaaa tgataaatct catcgattgc
    25981 cggttcatct tgtgcctgct tcagcatcgc ctcgtgttct gctttgaagt gattccaaaa
    26041 aaataacaca atttttgcat tggtaaatgg gcgcacgcgt cgagccatct ccatatcaaa
    26101 tccataatct aaaaaaacga tcagctgata atttgccaac tgttgtctag ctgatttaat
    26161 gaattccgac tgtagtaaat cttttccgga ccaaacatct aaagagcgga tggaaggcaa
    26221 aaaataaagt gatttatttt ttgcaactag cagtacgtca tttttgttca tcttttccgc
    26281 ctcactttct ctgtcacttt ttcctatgat agcctgttta aatagtaaaa agccagtaaa
    26341 acgtttccta tatttgtatg aaaatttaac atttgtatga aaatatgatg aaataaatca
    26401 ttcttctatt aaaattcgcc taaaagtttt ctgaatagtt gtaattgtct gaaaatttgt
    26461 tatcattagt cattgttaaa tattgctatt tattcttaat ataaattttg attgtgaatt
    26521 taaaagaaag aggtcattta tggaagataa aaaattgaaa aaacaactta gctccagaca
    26581 tatcaccatg ttagctttag gtggagcaat cggtgcaggt ctatttaaag gaagtggcga
    26641 agcgattggg atcgccggcc cttctgtatt gattgctttt ttgattggtg ggctgatttt
    26701 atatatcgtg atgaaaggtc tgggaaaaat cgttttaagc ggtggcgaca cgcatcacgg
    26761 attatctggt ttgatacgcc cttatctagg tgctcattct gctgatttta ctgattgggt
    26821 ctactggtct atgtggatta tcaatatcat cgctgaagct gttgcagctg cttccttttt
    26881 gcagttgtgg ttcccgcaca ttccagcctg ggtatttgtt tttatgttgg ctgttttaac
    26941 tacgatcatc aatctttatt cggtacggtt attcgcagaa acagaatatt ggctggcttt
    27001 tgctaaaatc agtgtcatta tcttactgat catttttgct acttatcttg tcggtcagca
    27061 aatgttgggc acaggcgttt tccctaccct tcagcaattg actgaccatg gtgggtttac
    27121 tcctcatggt atgaagggaa tcatcagttc tttgcttgta gttatctatt cttacggcgg
    27181 ttctgaactg attgctatca cggtaagcga agcagatgat cctaaacgtg cgattcctaa
    27241 agcaatcaaa ggtgtcatgg gaagaatcat ttccttttac atcattcctt tgtttttgct
    27301 tttgattctt tttccttgga atactttagc aggcacgaat gtcagtcctt ttgtgatggt
    27361 atttgaaaaa atgaatatcc catttgcagc ggatatcgtc aattttgtga ttgtccttgc
    27421 tttgttctca tctatcaatt cgggtgtgta tgcatcttca agaattttgt tcttccgttt
    27481 gaaggatcga aaaggttcct ctagaaaact agctgtttta aacaagcatc aagtaccgca
    27541 acgtgctgtt ttattttgta ccagtgttct ttatcttggt gtagctcttt cttattttgt
    27601 cggtgacaaa ttatttggct atctggcagg ttccctttcg tatacagtat tattgatctg
    27661 gatcattatc agtgcggcat cctttgtact ggcactaaga aaaggaacat tatgggataa
    27721 aggaatcagc ttttttgcat tagctgtctt aggattgatt ttccttggta tcctgttcac
    27781 gaactccatt ggggtaactg tattgactgg cctgctttac ctgttcattt atttcagtta
    27841 tcaaaaaaag aatgatgcgt tcgtattgac gaatgaatga aatcgataga aaattttatt
    27901 ttatttatgt ggaatagatg gagctattga agaaaggagt tgtgacaaaa gtcttgttgc
    27961 aactcctttt tccgaataaa cagtctcatg acctcttggt aaacggtgtt caaaaaccct
    28021 ctgtgcaaaa taattgattg ttctccatgc tcatgacatg atagaagaaa ggaatgaaaa
    28081 aaaggagttt gttttaaatg aaaaagtatt ggaagttttt gttgatgatc ggtgtttcaa
    28141 cggtagttat gtttggtatg atgtacttga atgtctatgc gctcgatcat atcttcttca
    28201 gtcgtacacg agtattcatg gcgttgatga tgggtgcagt catggcagtc atcatgctgt
    28261 tgttcatgtg gaaaatgtat gaaaacaaaa agataaatgt tatgatctta gccgctagtc
    28321 ttcttgtttt tgccggttcc ttgtttatgg tacgaagcca aactactgtc aacgacacag
    28381 cttggatgaa ggccatgatt cctcatcatt ctatcgctat cctggcaagt aaacgagctg
    28441 atttaaaaga tcctgaagta aaaaaattag cggaagaaat cattcaggca caagaagaag
    28501 agatcgacaa aatgaaaaaa atgatcaaag aaatggaatg accattatcc gtccaaataa
    28561 aaaaagagga tgttccagaa gttatcagct tcaagaaata agaagaagct acttctggaa
    28621 cgccgtgaat aaggggttgt gaaaaagctg ccctgcatca agtaataaga acagccaaac
    28681 aaaaatagct tctcctattt ttcgtttggt taggcttatt accgcagatg caagcttttg
    28741 aacaccgttt attaagaggt tgtaaaatca gcgttctgac ctgagtatta gagagacaaa
    28801 tggaaaaata gttcctcata ttttgccatt ttttgagcta ataccgaggg tcagctgatt
    28861 gaacaccgtt tatcgagagg ttgtgaaaaa gtcgcttagt ttcgcgtatt aagaaaaatt
    28921 ttgtgaaatg gctttccaca tttttgtata aatttggctt aataccgaga aactggcttt
    28981 tgaataccgt ttattcgtaa aagggattgt gacaaaagtc ttgtcacaat ccctttttat
    29041 gtttttattg ttctaattgc gctgtcttgc ttactgcttc ttgcggtttc acaaattttt
    29101 ttgagattcc ccaacctgtg aaggcactga ccatcgaaaa cacaggagaa agcaaactga
    29161 aaaatacgaa tggcaagtaa gtgagtgtag gaataccaaa tgtacttgct acgaaacttc
    29221 cggcaattcc ccaaggcact aagtagttga ttactgttcc accatcttct agcgttctac
    29281 ctaatacagt tggatcaaat cctgcttttt cataagtttc tttaaatgca ttaccaggca
    29341 gaataacaga taggaattgt tcaccaataa aaatatttac tccgatactt gtcatcaatg
    29401 ttgtaaaaat caaatttggt gtgttcgtca tctttcttga tactcgttcc atcacggttg
    29461 agatcaagcc aaattcgatc aacaatccac ctaatgacaa agttagaata atcaatgaca
    29521 cagtaggcat catactttcg attcctccgc ggctaagcag tgaatcgatt tgttggtttc
    29581 cagtatttga aacaaaccct gattcgatga ccccagccaa cttcgttaag gaagtttgtg
    29641 ggttttgaat aaaaatcatt cctactgcta cagcgatatt caacaaaatc gttaaaattg
    29701 ccggcacctt tttccatgca cacagtaaca ttaaaaataa aggaatcagt gaccagattg
    29761 aaatagtaaa gtttgcttct agaacatcag tcacttcacg aatagatgaa agacttgcgg
    29821 ctttacttgt gtgtcctaaa atcatgaata aaaagaaaga aacaacaaat gccgggacag
    29881 ttgaccacat catatgcttg atgtgtttga acaaatcggc atcaacaata gctgctgcta
    29941 gatttgttga ttcggataat ggagacatct tatctccaaa aacggcacct gaaacaatag
    30001 ctccaacaac cagcgctgga tttaagccta gtgttgtccc aataccgaaa aatgcaatcc
    30061 caatcgttga cataacagta aaggcactcc ctacagcgct acctacgatt gcacacacaa
    30121 caaatacaga agggacaaac cactggacac tgatcaattt gaacccaatg accattagag
    30181 ttggaatgat ccctgcttgt atccatacag caatcaatga accgactaaa ataaaaataa
    30241 agattgggat gattcctggt ttgatgccat tgatgattcc ttcgtgcagc caatctgtcg
    30301 ggtatctttt tatcactgca tacaacatga ccattccgat cgctgctaag atcgtgatat
    30361 taggtgagac ctttaatccg acaacgccaa tcgcaatgcc taaaacaata accattaata
    30421 caagtgttgc ttctaaaaaa cttggtgttc ctttttttcc catgataatc tctccttttt
    30481 tagttgttct tatgttctaa aaaagttaag atatcggatt actccgccgc aattcatccg
    30541 cgattcctcc tttataaaaa taaaaagacc tgttttaaac taaggacgaa tgactccgcg
    30601 gtaccacctt aattaaaaac agatctgttt ttcactttca aacttttatt caaatgtatg
    30661 gactttccat gtaattcgtc aattaggctt gatcggttca cagcgaccac cgactttctg
    30721 aacaaaacac ctaaccgcta ctgcgtgaaa agttgacttg tacgaagttt accttccata
    30781 agatcatttg tcaatgaaaa agaaaatatt ttttcatttt tctgaaaatt atttttataa
    30841 ctttcacaca caagtttatt ttttactaga aagtagcgcc ctcttgtttt tttcttgcta
    30901 ctatttcttg atgttcttgc ttgattcttt ctaatagctt tggctctttc aacagatcaa
    30961 gagctgtatt ggcgagtagt tctgctgcaa tcgcaataga agcaaggcct ttgtcacttt
    31021 tagcagcagc tttgaattct tctgtgtgtc cggcaatata tgtctcagag attgctaccg
    31081 taggctggat tgttggtatg acttggctga catttcctac atcagaagaa ccgagacttg
    31141 ttctttgttc cgtttctatt tcttcatctg gaacacctgc ttcttttgca tgctggaaga
    31201 ataagtcatc aaaagaagga gtaacgatga catcatcaac tgcattttgg aacaatccaa
    31261 actcaaaagt cgtgcccgta cttatcgcag cacctcgtac gatattttcc actttttcat
    31321 atactttatc taatgtttgc cgatttcctg cacgtaaata gaaacgtcca gcagcgtaat
    31381 ctggaatgac gttggcagct actcctccgt ctgtaatgat cccatggata ttgacatctt
    31441 ttggcaattg caagcgtaga gcgttgattc cattaaacac ttggatcaat gcttccaaag
    31501 catttactcc tttttcaggt gctgctgctg catgggaagc gacaccgtaa aatttgatgt
    31561 cgactggatc attcgctaaa gattctgtcg ttttcccata acgaaaagct ggatggacgc
    31621 ataacgctac atcgacatcc tcaaaaaaac cttctcgaac aaagctgcct ttagctgatc
    31681 cgttctcgcc cccttcttcc cctggggtgc catatacacg gatttctcca ccagtttcat
    31741 caatgaattg cttcactatg ctggcggcta atacggagta agtaccaaac aaattatgcc
    31801 cgcatgcatg accaatacct ggtaaagcat cgaattctgc caaaaaggca attgtgggac
    31861 cgggtttatt ggaaatataa cgtgcatcaa aggctgttcg gtgtccagct acttcttttt
    31921 ctacccaaaa cctttctttt tctaattgtt taatcaacgc tgctgaggaa taaaactcat
    31981 agttacttac ttcaggatgt tcgtggatat ctagagcaag tgcttgatat tctggtaaac
    32041 gatattggat tagatccttc aattggtttg ttgagattgt tgttgtcata aaaaattcct
    32101 cctatgctag tcatttttct tattttctcc cgaatgtttc ccatgcaggc acgcttgatc
    32161 cttttgatgt tttctcgatt actttgactg tgtcttctgt ttgataagcg tctactattt
    32221 tttgatacgt tttattgttt tcatcttctt ttcttgcaac gatgatattc acataaggac
    32281 gtgcatggtc atccactggt tctaagaaga tggcatcaga agcaggatta aatccagcat
    32341 ctacagctac accgctattg atcactgaaa tgtctacatc tgacaaagca cgtgctgttt
    32401 gggaagcatc taattcagaa atgtctagat tttttctatt ctcggtaata tcagaaacag
    32461 tgggcgcttg tccttttgcc ttatccactt tgatcaatcc agcagtttgc aataacagaa
    32521 gagctctgcc gccatttgta acatcattag ggattgctac ttttgctccg tctttgatat
    32581 cagctacatc cttcaaagtg ttagaataaa ttcccagcgg tgcattgaca gtatttccga
    32641 tcgatactaa atcagttccg tgttctttat tgtaattgtc taaaaagaat tgatgttgga
    32701 aagaattcaa atcaatcgat ccatctgcta aagccacatt tggttgattg taatctgtaa
    32761 atttcacgta ttccaaatta atgttctcct ttttcaaacg gtctttcacg ttgtcccata
    32821 catctgtatc ttctccaata atccctagtt ttacgatagt ttcttttttg tcagttgtat
    32881 ctgcttttcc ttggctacac cctgccaacc aaataattgc tacaaatgta atgactacaa
    32941 aacgaataat ttttttcatt ttttctcctc cactttcaat gtgtaatttt tttaatcagt
    33001 tgattactga taaattgact taaaaaaact aagattagaa tcaataatgt agccacaatc
    33061 gttacatctg tttggaatcg attataccct ctcgatatag caagattccc taatccgcct
    33121 gcacctactg ctccagccat ggtagtcagt ccgatcacgt tgatgatagt caacgccgag
    33181 acccgaatga taccaggcaa accttctaac aaatacacac gtaaaatgat accgattgga
    33241 ctggttccca tcgcttctgc cgcttcgatc accccaggat caacttctag cagtgcgttc
    33301 tcgatttgac gagcaaaaaa cggaacaact ccaaaaatca atggcactaa agctgctttc
    33361 gcaccaatcg tcgttccaac taagaaacga gtagtcaatg ctaaaagaga aagcaagata
    33421 atgaaaggga tggaacgaat gatattgatc agcttatcaa gtatttcaaa tagcagacgg
    33481 ttagctagta atccattagg gcgtgtcact actaacgtca cacctaagat gattccaatc
    33541 atcccgccaa tgaatgctgt ccaaaaagac atatatagtg tttgcttcgt cgcttcgaca
    33601 aattcatccg gaatcaaata aacattgggc aaatatgttt taaagaaatc agtcatttta
    33661 tcttcctcct tcgatcactt tgatacgact atctaaatcg tttttttgta aaatatttat
    33721 ttttacacca gatttgatca aataatccaa tgcttttttc cgctgtttgg tttctccagc
    33781 taaagtaacg atcaaactcc ctaacggcgt ttcttgtaat aactctacat tgccatataa
    33841 aatattcgct gttacttgat atttgctgta cagatgagcg attagaggtt cgttcgtctg
    33901 atttccaata taacttaatt ccaccagcca tttattggcg atctgatcgg caaaagcaga
    33961 atgggcaatg atcgtttcta atgcttggtc aagatgagta gctgttcgaa taaaatcttt
    34021 ggtcaattct tcttcaggat tgctgaagat ctggatgctg ctaccttttt caacgattct
    34081 tccatcttcc ataaccgcta ccttgttgca gatttctttg acaacttgca tctcatgagt
    34141 gatcaggacg attgttaaac ctaactggcg attcaatttt tttaacaatg caaggatctg
    34201 aagggtcgtt ttcggatcaa gtgcactggt tgcttcatca cataataata ccttaggttc
    34261 attcgccaaa gctcgtgcga ttgctactcg ttgtttttgt ccccccgaca gttgtttagg
    34321 ataagcagat gctttttctt ccaaaccgac aagttccagt agttcattca ctttttgacg
    34381 acgttcctgt ttcgattttc cagaatactt taaagaaaaa tcaacgttgt caaaaatagt
    34441 tcggctttcc attagattga aatgctgaaa gatcataccg atggcctttc tttcttttcg
    34501 caattctttg ggagagagct gactcagatt cgtctgattg acaatgactt ctcctgctgt
    34561 tggtttctgc agcaaattca tcacacgaac taacgtactt ttccctgcac ctgagtaacc
    34621 gacgattcca taaacatcgc ctttttcaat cgttaaatct acagaatcta ctgcctgtat
    34681 ctctttttct ttttgtttga aacatacaga aacatctcgc aattcaatca ttaaaattcc
    34741 tccttatcta aaaaaagcgt cctcaagaaa atgaaaatgt cattttcttg aggacgcttt
    34801 tttgcgtgtt accacctcat gttcaagctg tgttcacaca caactcctct tcaagtacga
    34861 tcatactcta gcaagatatc ggtcgcagcc gtcatcgcct tacatctatg ctcgggatga
    34921 attgctcgat ggccattttc caatagtcgt tccttgcttc ttctcagcta ccgaagctct
    34981 ctgtcaaagg ttcttctatc gtacttacca tttcaatgca aataatattc aaaaaaaaag
    35041 tccttttact aacataaact atgtcagtaa aaggacgacg tatcgcggta ccaccttttt
    35101 tcggaagttt ccttccctca agcccaacat ctcaaaaaga gagattggcg ttcgttttaa
    35161 cggtcgaaca gttccgaatt tgcctactta tcgacaaatt attctccaag gccattttca
    35221 gcttttctta cttgactctt ctcaccagcc agagcctctc tgcaaagttt ccaaacttac
    35281 tttccttttc aacgaaatcg atattttgtt gttgggatta atctacatga ggaaaactga
    35341 aaagtcaagt acgaaattca aattattttt tctatattta tttttctgtt tttttattta
    35401 acatttcaat aacagttcga agatcttttc cttccgcaag aagtttcaat ttttcctttt
    35461 cttcgacggt ataatttcgg acaaaactgg aatcaggtat caaaacaacg cctaatcctg
    35521 ctcgtgaagc agctgttgct cctactaaag aatcttccac tgcaaaggct gaactagctt
    35581 ctaatcctgt tcgttccaaa gctgtcagat aaggaatcgg tgaaggcttg ttctccgaca
    35641 cttcgtctcc gaatgtaaac gtgtccaagt aatcagctaa tttaaagtga tccaagatat
    35701 ctgttgctct tttcgtaaca gtggtcgtag caatgcctag tttcatatga cgttctttta
    35761 agtctgccaa tatttctttt gcatatggtt ttaagtggat ggctccgctt gtcagctgtt
    35821 gataaaaata ttcttcacgc ttcgctcgga cttcctgtac tttttcaggg ctacctgctg
    35881 ctttcactaa aatatcaaaa ctttgtttcc agttttttcc gccccaagcg tcaacgattt
    35941 cttttgggat atggatatca aattgctgaa atccccaaag ccagccatca cgatacgttt
    36001 tttctgtgtc aactaacaaa ccatctaaat caaatattgc acctttcatc tatacattaa
    36061 tcctttccaa aacaatacac atagtttagc acgactggtc caaaaagaac agaagcaaag
    36121 aaccagcctt ctcgtttata agagttcttg gaatcgacgg atatagattt tcttcacagc
    36181 tgtcacaagc gccagataag cgatgactgt agcaaataac cataaccagt aattgccagg
    36241 aagtggcatc aagcctaatt cagcaccaaa acttgtaaaa ggaaggacag aaccgacaat
    36301 aattcctaat gtagtgataa caaacatggc aaatgttgcg ttgctttgga taaatggtac
    36361 tttcggtgta cgcagtgcat ggagcactaa ggtttgtgtc catagtgatt cgacaaacca
    36421 tcctgcatgg aataatgcga tgaaaatcac tcgctgtgaa ctatccaatg tatggaaact
    36481 tccgcccact acagcaggac aaataacaaa atacatcaac aagtaagtag taatatcaaa
    36541 tatcgaactt gttggaccaa agtaaaccat gaattcccca atactagatg cattccattt
    36601 ttttggctcg tccaaatatt ctttatccat ccgatcccat ggcaaggaaa tgcaggaaac
    36661 atcatagatc agattcaaaa acaaaagttg taaaggcagc attggtaaaa atggcaggaa
    36721 cgtacttgca atcagcacgg aaaacatatt gccaaaatta gaacttgctg tagccttgat
    36781 atatttcatg atattcccaa aagtttctcg tccagaaaga aggcctcttt ccaaaatcgt
    36841 caaatctttt tctaaaagga tcacgtctgc agattctttc gcaatatcca cagctgtatc
    36901 tacagaaata ccgatatcag ccactttcat tgcaggagcg tcattgatac catctcctaa
    36961 aaatccgaca gtatgaccat ttttacgtaa agctgtcgtc aaacgtgctt tttgctgagg
    37021 attcaatttt acgaatactt catggtcttc agctacttgt gctaattcat gatcactcat
    37081 ttccgagatt ttctcaccgg taaccaattc attgatctct agacctactt gacggcacac
    37141 tgaacgtgta actaattcgt tgtccccagt cagaactttt acagctacac catgttcttt
    37201 cagagctttc aacgcttgtt tcgttgtttc ttttggtgga tcaaggaaag caagataacc
    37261 aatcaaaacc atgtctgact catctttgac tgaaaattct ccaacgacgc ttggattggt
    37321 cttttgtgcg acaccaatca ctcgtaagcc atcttcatta agtgactgta cttttgcaag
    37381 tacggcttct tttcgttctt ttgttaatgg tgtaacagta ccgccaagat caataaattt
    37441 agaaatgttc agcatttctt caatcgcgcc ttttgtgatc atttgtgttt taccagctgg
    37501 atcagcaacc acaacactca tacgacgacg ttcaaaatca aaaggaattt catccacttt
    37561 ttgataattg atcttatctg tatctaaagt ttctgttgct tcatcaatga tagccacatc
    37621 taacaaattt ttcagccctg tttgatagta gctatttaga tacgcatggc gcaaaacacg
    37681 atcatcttca tttccttcac aatccatatg atattcaaga atgattttat cttgtgtcaa
    37741 tgtccccgtt ttatccgtac ataacacatc gattgcacca aagttctgga tggcattcaa
    37801 atttttgatc actgttcctt ttttggccat cacacttgct cctttgacca aattcgtcgt
    37861 aacgatcatt ggaagcattt caggtgttag accaactgct acagatagtc caaaaagaaa
    37921 agcttctagc caatcccctt ttgtaagccc attgatgacg ataactgctg gagccatcaa
    37981 tgacataaat ttgatcagta gcatggatgt tttatggatg cctaattcaa agcttgattg
    38041 aattggtttt tcagatagcg tttctgctac atgaccaaat agtgtttcat ttccaacaga
    38101 aacaatgatt ccttccgcac ttccactgat gacattactt cccataaaga caagattttc
    38161 gtaacttgtt tcgctttgct gatcgtcttc acatactttc gcatgctttt ctactggata
    38221 gctttcccct gtcaatgctg cttgggaaat gaataaatcc tttgcggaca ataaacgcat
    38281 atccgctggt atcatatccc ctgctgaaag ccgaacaaga tctccacaga cgatttcttc
    38341 cataggaatc tctgtgtact ttccattacg ttttacagct gccgttactt tcacaagtga
    38401 tttcagtttt tcagccgctt gattggaacg tacggactga atcaacgtca ttgtcccgct
    38461 gacgataacc atagtgaaga taatggctac accgaaaaga tctttatctc caggagctgc
    38521 taatacatat tccgtgatga atgaaataat tcctaatgcg atcaacacta atgtaaatgg
    38581 cgtgatgtat gctttcaata tttctacaat gaatggtgtt tttttgccat atgaaatcgc
    38641 attttcaccg taattttctc ttgaacgttc gatttgttct tctgtcaatc catttgctga
    38701 tgcatggaat agttcaaata attcttgttt cgtttttcca gcaaaagctg catagatttt
    38761 ttgtgtatct ttcttttttt ccatctcaat ctctcctttg tgtccaaaag acagctgttc
    38821 tcgctgcttt tggtctttag ctattcatcg gatcgataga gaaaaaactg aaaggggctg
    38881 agaacagata gataatagcc ctggcctttc ctctagatcc ttgtttttcg aatgactgtc
    38941 atccatttgt tctcatctcc tttactaagt aattttaatt catgacttcc aatcattttt
    39001 cgaatagtta tttttccaat tagaccacct cctttttatt tttcaccgat ggggatttgc
    39061 ggcaacaaaa aaacctctcg ccaaatactc agcgaaaggt ttgaaaaagc atattaaaaa
    39121 aagcttctca gatctcatcg ttgagttttg acactgtaca acgtaaaaag cagatacctg
    39181 cttttagcta ccttatgaaa agccttatct cgacaattcc tgttctaccc attggcgtct
    39241 ctcgacgttt ttgggcagta gcctatgttt gtataggagc ctcacctaac aaatcttatt
    39301 cggttttcct tcgttaatat aacaactaaa aaagaggatg tcaaatcaat tttccatgtt
    39361 tttcatactt tttttaaaat acacacgtta tattcctgtc tcacgcggtt ttatttgtct
    39421 cattttccac ctcccgctat aatagaaata tagtaggaaa cgaggtgctg ttatggaaag
    39481 aacacttgtt attatcaagc cagacggcgt gagacgccat ttagtcggaa gcatcatcca
    39541 acgttttgaa gcaaagggac tggccattgc tgaaatgaag tttgaaacta tgactcctga
    39601 attagcaaaa gaacattatc agcacttgac tgagcgcagt ttttttgatg aactgatcga
    39661 ttacatgact tccggtcctg tcgtttatct tgtgctggtc ggcgaggaag tgatcgatat
    39721 cgtccgtaag atggttggag caacaaaagc tgccgatgct gttccaggta cgatccgagg
    39781 agactacgcg ctgccgggga ctgaaaacat cattcacgct tctgattccc gtgatgctgc
    39841 ggtaaaagag atcgcccgct ttttcccgcc attcgatata aacaaaccgt ctgaaaaagc
    39901 ggcgaagtga tacaaattct aaactgctgg tctagttgct gggttcagca atggccagca
    39961 gttttttctc taaggacctc cgttgattct ttcctaacat atattcgagc actgtgaaga
    40021 attcttggta gtctttcgtc ttttcttccc ctaaagcagc agaaatcaat ctttgataat
    40081 aatctgccat caatgtttca tagatcactg tacttctttc tttatgtgat aagctccgtt
    40141 tcatatcttc attcacacta cgtgcctttt tcaaccgacc attttctata taaaacgaag
    40201 tcaggttcat taaaaaagta aagagatact gctcatgatt gttcaactgt gctaatcgtc
    40261 gcattctttt tttgattttc aaataattgt tctcaatgaa ccgatcactg aatatccaag
    40321 gaaaatcagc tagaatagat agttcaaatt gaccatagtc tggtatctgc atcaagtatt
    40381 cttcaatgat tcggcattca taacgtaaaa attcaaattc tgtctcagat acttctgttt
    40441 tttcgaccat gtgaagattc aaaaccaaat aatacagatc ttctgttttc ttaaaatgcc
    40501 tacggttatt gctgatctct ttataaggat caaagctgtc ttcttgtacc cgatctagca
    40561 ttcgtctttt tttctttaac tttattggtt catccagctg gtttcgcatc cattcatatt
    40621 ctgcaagtgt catgttcatt cgctctaaaa atgatctcaa tgttacaaat gacatttttt
    40681 tcactgaatt tgtctcaatc ccgactagag aagtcttaaa acagattcct tccgctaatt
    40741 gggactgtgt caacttcttg ttgactcgaa gtttcttgat caattcacca tcatacataa
    40801 tagttctcct ttttaattta aatttttcca cattcaataa atagatacgt taaaaaggaa
    40861 aaatacacga aaaaaataaa aaaatgtttt tttcataaaa aataccaacc ttcaagcatt
    40921 agaatgttgt ctaactactg aaggttggtt tgattgttta ttgattattt ttttgctgtt
    40981 caacgtctgt tcttgttact ggatataagt tggttgttgt tttattacca ttttttgaaa
    41041 agacacttgt cgaatgttcg cctttttgct tttcgattgc ttcattacgt tccgcttcat
    41101 ttttatagtc atattttttc ggatcaatcg tctcaaatcc gtttggcaca taaaaccgca
    41161 agagattttc ctgattcagc ttatctgaaa gacttaacgc agtcttgact ttttcctgat
    41221 ctttggagat ttcctcatcg attcctgctg ctttcgtatc gatgatttct cctgtctgat
    41281 tattgtaagc ttttcctcct aggacggtgt atttgggtgt cacaaagttc ccatttcgaa
    41341 aagcaactac ttgatcatgc tgagaagata ataagtctgt accaaaatga agataatctt
    41401 tatcatcgat tcccaataga tgtagtaatg tcggcaaaac atcgatttct ccaccgtatt
    41461 catgattgac tgtcccttta gtataaccag gcatatggat catcaatggc actcgttgca
    41521 tctgtgcgtt gtcaaattca tcccatgtat ctggatcttt accaagagcg cttgctaaat
    41581 ctttattatc tgtattcgag atcccaaaat gatccccgta aatcacaaag attgaatttt
    41641 ggtaaatacc agattttttc aaatacgtaa aaaattgttc caatgattga tccagataat
    41701 gtgccgttcg aacataatta tctactgtgc tattgccagt attcaaagat ggaaagtcga
    41761 aattcttgtc atccgtataa taaggcgtgt ggttggttac ggaaagaaat ttcgtgtaaa
    41821 acggctgttg tagatgctca agatattggg cagattctct gaataaatct ttatctaata
    41881 tcccataacc taacgtttcg tcggattcat caaaataaga tcgatcaaag aaattgtcat
    41941 aacctaagtt tttatataca tgatctctat tccaaaaact agctacattc ccatggaaaa
    42001 cagcgcttgt atagccttgc ttttgtccaa ggatcgctgg tgcggcttga aagacattgt
    42061 ctgacccaag ttctgtgaat aatgagcctt gaggtaaacc aaatgtgcca gtttccaaca
    42121 tattttcagc atcacttgtc tttccctgtc ctacttcatg aaagaaattg tcaaagctga
    42181 tcgttgcttg attctggaaa atactattta aaaacggtgt gacttcttgt ccgtctacct
    42241 tcatattaat caaaaactgc tggaaacttt ctaaatggag aacgatcaca tttttccctt
    42301 tagcgatccc aaatgtttcg ggatttggct ctgcataatg tttctttgta taatctaaaa
    42361 cttcatcaat cccattactg cttgcatgcg cgcgaacact gctagtcatt cctgttttga
    42421 ttccgtcata aatagtatac gcatcgattc ctagatattt gacaatatat gaacggtcaa
    42481 atgttcgctg cagcaattgt ggacggttgg cttcgctcag cgctagattt cctgaaaaaa
    42541 taagccctga aagacaggta acagcgatag ccatcggttt gccgacaggg ttgcttttga
    42601 taggtatttt cttgaccttc aaccaaacca gtagcccaat gaacacagcg atatccagcc
    42661 agtagatgac atcatggggc ttcatcaaag aaaaggagct tcctgacaat ccttgcgaca
    42721 ctttcgaaaa gcctaataca gatttgactg taatgaaatc tgtgaattcc cgataaaaaa
    42781 taatattcaa gtacaacaaa agcgtattta aaatatcaat gatcaaaaga atagctaacg
    42841 ctggctttgg ctttttaata tacaaagaaa ggccaaaaaa caataaggta gtcgcaattg
    42901 ggttgatcca caagataaag tactggatcg taccttccac acctaatgaa aaatctgcat
    42961 agtaggcaat gatcgtcttc agccagaaaa agaaaacagc tgtcaaaacg attgctaaac
    43021 gattttggca caatttttta agttgtgtca cgtatatcac ttctctctat tcacattttt
    43081 tcttattttg ctcctgaaaa atgaaaattt cctaaaaaag ttgttatctt aagatttttt
    43141 tattctttct aaagattatt ccgtccactt ttcacagcat aaaaccgctg cgatgtaagc
    43201 gattaaacta atgaagagat aaaagtcttg tggtgaccgc ttcactccac ggtattcaaa
    43261 agccagtttc tcggtattcg tacaaaaaaa gaggctgtga caaaattctt gtcgcagctc
    43321 cttatttctt gatgcaggac agctttttca caacttcttc cttaaacggt gttctcctgc
    43381 caactcctcg gccacaagtc acaatattta taaaacatga gaagctgttt tttgaaattg
    43441 ttccttgtgg ctcagagtta aacggcaggc tcaagacctc ttgatacacg gtgttcaatc
    43501 agctgaccct cggtattaga ttatgtcagg acgctaattt tacaacctct tggtaaacgg
    43561 tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat aaggagctat
    43621 tttttgaaat tgctccttgt ggctcagagt taaacggcag gctcaagacc tcttgataca
    43681 cggtgttcaa tcagctgacc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta
    43741 ttttgccgtt tgtttctcta atactcaggt caaaacgctg atttcacaac ctctgttttt
    43801 cattgtccat aggattgata ttgtccttgt gatgactcat tcgtaataat tgaggcttga
    43861 ttttcttcac ttcccgttat cgtttgtcca ctgccagaat ctgaaggact tgttcctgtc
    43921 gactcgtttt tttgagaaga gacagttgtt ttcttgactt gttcatttgt ctttttccat
    43981 tccgctaaac gatcggcgac cacttttttg tctacttttt cagaataagc ggtgatcact
    44041 tttccatttt tcaaacgggc tactttgtcg ccatcatgag tcaaaagcaa aacggtcaaa
    44101 tgcttgtctt taaattgata ttgtctgaga gaaatcgcac taagtacgtc atcgatatta
    44161 ataaattgat tttcttcttt tccttttggc aaataaatgt ccaattcgcc tttttcgtaa
    44221 ttggcagtta ttttatactc cgcccaccaa taagttgaga attctttttt gaaattatct
    44281 actgattctt tgcttgctac gattttttct gtttctttct gagcaagatc tgctgtcatt
    44341 tttcctgaaa atgctgcgga taaaataagc agtaagaaaa aaccgaataa ccaaaagaga
    44401 agaaacggtc tttttttcct catgctttcc tccactccta tttaattttg actattctat
    44461 tatatacaaa aaatgaaata gaaagacaaa aaagttgaaa aagatcgtaa ataattgaac
    44521 ttctttttca caaaatggta agaatggaga atttcacgca tttgctctca actattcttt
    44581 cttaagcctc aaatccgttt ttatctgata cgtctttgaa ccaatgaccc gattttttga
    44641 ttgttctttt tccatcttgt gctaagtcta ctgaaataaa gccatatcga ttcttatatg
    44701 cattggtcca cgaccagtta tccatacatg tccacatatg atagcctaaa caattggcac
    44761 cttcttgaat ggcttgatgg acatatttca gatgagattg gacaaattcg atccgataat
    44821 catcgtggat catgccatct tctttgataa atcgtgcttc gttttctaca cccataccgt
    44881 tttcagaaat aaagcagtcg atatttccat aatgatctcg tacattgatc aaaatatcat
    44941 agattccttg ttcatagatc tcccatccgc gataaggatt cattttacga ccaggcattt
    45001 cataattgtc gaaataatcc tcaggcattg gaccgttttt agtatcgatt ggtgtttctt
    45061 ttgcttttac ccggcgtggc tggtaataat tgatacctaa aagatctact gtattttctt
    45121 tgataaccgt caaatcctct gcatggatca caggcatgag atcaagttct tttactatct
    45181 caatcaattc acttggaaac tcgcctttta cagctggatc aaggaatgaa cgattgaaga
    45241 acgcatctgc tatttgcgct gctttaacat cttcagggtt tgattcgtct cttggatagc
    45301 ttggtgtcag attcaagatg ataccaataa caccaccttg ctccatttca tggtaagatt
    45361 tgatggctaa cgctgaagcc aacgcttcat gatagccaac ttggatcgct tttttcatat
    45421 ctgactcttc tggataatgc catcgatata aataacctgc ttcgactgga acgattggtt
    45481 cattatgagt aaaccatttt tttactttcg aaccaaacaa ttcaaaacaa cttttggcat
    45541 atttttcata ggcttcaacc gtttctcggt tcaaccatcc gcctttttct tgcagataca
    45601 tcggcatatc gaaatgatac agattcacaa agggttcgat accgttttca atcaactcat
    45661 caaaataacg atgatagaaa cgaacggctt cttcgttgat tgttccagat tcatcaggta
    45721 tcaaacggct ccattggata gatgttcgaa atgaattatg cccagtctgt ttcatcaact
    45781 gtacatcctc ttgatacttc gtataaactt gcgaggtttt ttcaggtcct acttgatcaa
    45841 agaacttttg cggttctttt ttgtaccaat aatcaaaaat gttctctcct tttccatcgc
    45901 ctgaaatcct tccttctgtt tgtgggccac ttgctgcaga accccaccaa aaatttgctg
    45961 gaaattgata ttttttcatc aaagcacctc tttttctttt ttagttcgtt ttagttgatt
    46021 ccatgtatga tattttccat cacaatcact ttttcatttt cttcctcttc ttttttgatt
    46081 cgttttaaca gctgttctgc tgctcgttcc ccgattttat ctgttggtgg cgtgatcgtg
    46141 attttcggaa ggatactcag atcgttttta tctaaataat catagtgact gaacgcaata
    46201 tccttcccga ttctgacacc ttcttgattg aaacattcga tggcaccaat cgacatattg
    46261 taattgctga caaaaatagc agttgcccct ttttctaata atgcttttgc cccgtaatag
    46321 ccgctttttc tagaatagtc tcctgtaaag acctctaatt ggatcgatgg atcattttgg
    46381 accgcttctt tggctcctgc taaacgttct cttgctacgt agtctgtttg agtggcagca
    46441 ataaatccga tttttttatg tccttgttca ccatgatgag cgatcacttt tgctacactt
    46501 tctcgatcat tcactaatat tgtatcaata cctgctggtc cttctggaca attcaatgcg
    46561 ataactggaa tactcatttt tgtgagcatt tcaaatccag accactcttc ttcggataag
    46621 aagacaatca gcccatcgat cacatgcttc atcaggtatt caattttttg ttcaattttt
    46681 tctggctgat cggcaaaccc gcttaataat aagctatacc cattttcttc agctactctt
    46741 tcgatccctg agacaacttc cgacccaaaa cggctggaaa tcccgtttat caaaagtcca
    46801 atagagaagc ttcgattatt cttcaaccct ttagcaatga tgttttcttt atagtccaac
    46861 gctgaaatcg cttctgctat ttttttcatg ttcttttcct tcagttgctg accattcaag
    46921 taacgggaaa ctgttcccac tgaaacacca gccatcttag ccacatcacg gatcgatgcc
    46981 atacattctc actcctgcca gttacatttg atttctcttt cttgttgttc gattatgatg
    47041 accgcattct ttgcatagtg ttgcttgtca tcaatatcca tataactaaa gtcttcagta
    47101 gagcaacttc ctctcactat taattatact taaacaagta tttgaaccgg ttcattttta
    47161 atatagcatt tttttaaaag attgcaacac ttttttaaaa cgctttctga aagtggcgga
    47221 ttttttgcct ttttaaaatt ttattctaaa atttactcta tttgtgggtg ttttgtgcgt
    47281 ttttctcttc catccgtttg aagttttaca ttccaatggt ataatttctt tatcctatca
    47341 aatgggggaa gtattttgaa gaaattaatc gtactattta ttttgtctgc gggacttttg
    47401 acattgactg gctgtgattt gtttggcggc tctgaccaat cctcggacga aacaaccgat
    47461 tcttctgtct taaaagcgga aaaagcagat accaccaaca gtgtatatac gctaaaagta
    47521 cgtaaagata tggatttatc cgcgggacag ttgacattta cagataaaaa aatggaatgg
    47581 acacgcacgt atcagacaca aggaactgat tcttctgaat catctgaaga aaaaacagtg
    47641 ctgacagata tcaagatcac taccaaagat gacgaatata ttatttcagg aaaagaaaat
    47701 aaaaaagaaa caacagtcat atttacaaaa atcggtaatt accgtttgaa ggatgaggat
    47761 ggcaacatct acagtctata gtgccgtctg ttggatcttc ttttgtttct ccccttcttt
    47821 tctgctatag taaagaagca agatgattgt aaaggagacg aaatggatga tcgatagtcc
    47881 tgattattgt acatgtaata acaacaaaaa tatccataca gaatttaatg accaaggaag
    47941 cttgagcata tgcgacaact gtcacaaact agtcaaagat tcatttaaaa catttgaatc
    48001 ttatcctgtt caaaattatg taaaaagtag aagaagataa acaaatccaa gcaaaactca
    48061 ctatggctga tttttatgaa cttcaaactc agcttcttcc tttgatcaag cttcatcagt
    48121 aaaacaaaca gtggatcatt tcagtttaca aatcaaagag taaagattta tttatttcaa
    48181 atatttgaat ataaaataac caaacgatag aaaggcttat tctacatcac gtagtaattt
    48241 ttctttcccg tcgtttggtt ttttattttt agcctaattc ttgacctttt gcatgctggt
    48301 tatccgtaaa atattttcca gcaggctttc cttccaattc agctaatttt tcttggatct
    48361 gacggatcac cttttttgca tcttctggat tttctacaaa atcgaattta tccccatcca
    48421 cttgtatttt aggacataca tcaaaatctt cataccattg attatagcgg gtgttcaacg
    48481 tttcataata ggtatataac gtctcatcgc tttctagctg ttcatagtca cgcccacgtt
    48541 ttttgatacg ttctagcatc gtgtccaaag aaactttgat atggaccatc aaatcaggcc
    48601 gtttttttgg agcaacatca tctaactcgt ttagcatcgt atctaataag ctatcgtact
    48661 gctgtacttc gatatccgtt acccgaccta aatcagcgtt tagatggaac aacaggctat
    48721 cttcatagat ggatcggtct agcacattgt catcgttcac taaagcattt ttcattgcta
    48781 aaaatcgctt gttcaaaaag aagatttgca ataaaaaagc gtactgctca ggatttgaat
    48841 aaaagagcgg caatacttcg ttatcttcga cattttcata aaatggttta gaatcaatct
    48901 cttttgcaag catatccgtc aaactgctct tacctgcacc aatcgttcct gaaatcaaaa
    48961 tcactgacat acaacccttc ctcttctata agtactcgcc tacgtaagtt tcaacaggca
    49021 atgtttcaaa atcatattca cgtacagctt ggtctgattt taaatacgta tggagacttt
    49081 gtgtaatcat caagttcgta ggtaccgtct cttcttgtac aatgatcatc ggttttttca
    49141 acatagttgc cacacctaat tcgtaagccg ttcctggatc gattgtttga tgctcaaagt
    49201 caaggatagc aacaacaaat tctgcatttg tcagttcttc catatctttt tcgtatactt
    49261 cctgcgccca tccagcgctg aatagttcat aattgctttc ttggtgatga cgtggagagt
    49321 agaaacttgt gactgttttg ttttcctcca acgctttttc gatacgactt actcgatcga
    49381 tttgctcctc tgaaaaaaat ggtcctgcta aatatatttg actcattatt ttgcctcctt
    49441 caataataac tgatttttaa tagatactgc tcgtaattca ttgaccagtt ccaatggatt
    49501 gttggaaaag attacttgat ccaaccattt tttatccata aaatcctctt ttgcaaaagt
    49561 ttctaataat tgttgcaagc cgctgtaata accgccaata ttcaatattc cgattggctt
    49621 gtcgggcgtc agaccgattg ctatccaaga aagaagttgt gagaattcct ccaatgtacc
    49681 tagacctcca gggaaaacga ggacagcatc tcccttttca aataataata cctttctttc
    49741 atcgatcgtt tctgtaggaa taaatgtagt aacatctttt ccaggcaatt cagtttcaaa
    49801 aagcccattc ggatagatac ctgtgacttt tccgttattt tccagtactg ttttactaat
    49861 cactcccata atcccggagt atgaaccacc gtaaaccagt tcgatttctt cttgtgccat
    49921 gtattttcct aacgtttgcg caataaattt gtaactttct ttattaccaa aacgggaacc
    49981 gcaaaaaaca gtaagtttca taaaaaaaca ctccctaact caaaagtcta tcttcattct
    50041 acacgtgttt ttttaaaata aaagactaga ttttgtagtt ttttagtggt atataggata
    50101 gacgccacta tatatagtat ttagaaaaaa atcatttttc agccatttta tagataaaac
    50161 aaaaataaat aacgtgtttt catcgatatg aaatgactta cttatagaag atactgggat
    50221 ttcgtcctat atgccaataa cgtgtattat tttaaacaac aaatacttgt ttcttttatt
    50281 aacgaaaaaa tcattttgtt ctcgtgttat gtttcactga taaatgatgt ttctctaaat
    50341 aaaaaagccg acgaactaaa cgatcaaaat ttgatcattt ttgttcatca gcttctccta
    50401 aaaatctttt tgttaacgat aaatagctgg atttgtaaaa atatcacttg aatcgtcact
    50461 gctggaagaa aattcagtga taaccgcacc tgttgcgcct gcctggaact agtgtttgat
    50521 attgggctcg aacgtgtact gatctctagc atgaagaata atttcttttt ttactgtata
    50581 ataactttct ttcctttttg gaatagtaca ggaaagtttt tttgtttcct ttccttcgac
    50641 ataaggtata ccagaccctt tcgacaacga aaggttctca tttttccttc atcgtcattt
    50701 caaggagggt gccgatgctc tggacagttt cgatcgctcc taaaccaaaa tctgtatatt
    50761 cgattctttc taactctgat tctttcatac cattcctgtc tcaaggtaca tctattgtac
    50821 ttttttcttg taatccattc tgcctctcct aactaattgt caaatcatcc caagatatgt
    50881 cttcttcctc tttttcttct tggatgattt ctgcattcgg atctactggt tttttcaata
    50941 ataaaatggt tattcctgtg acaaaagaac cagctaacaa tgcaccaatg aatgcccatg
    51001 gtctggtcat cgtagcgact gcaaacattc ccgatgccgg aatacggcta tctgcgcccc
    51061 aaaacatgct taatccgcca cctactgtcc caccgactcc cgttgctatg acattgcgtg
    51121 ccaagtcgtt caatacgata gggaaacatc cctctgtgat ctgacagatc cccataggaa
    51181 aagcggtctt caatgtatcg atttcttgac gattgagtat atttttacga atgattttcc
    51241 ctaagaaata agcgatcgtc aagccgaatg gggttaccat cgaagctaaa atcaatacag
    51301 tgattggttc atagattcct tctgaaaatc gtgccacacc cccttatcca gaataaatgg
    51361 taatccagaa acagccgtgt atctgcggta ataagcctaa ccaaacgaaa aatatgagaa
    51421 gctatttttg tttggctgtt cttattactt gatgcagggc agctttttca caacctcttt
    51481 ttttatttat gtttcataat caatatggta aggatgcagc tccgtatggt agaccgcctc
    51541 cgaatattca atcatttccc caatttgcgt agacgatttc cgtatccgtt tgatgccgat
    51601 ctgtgcgtct gttttcagaa ttccttgttc ttcttctgtt aaaaatactg ccgagaaatc
    51661 atcagaaaat gagtcaatag tatatccttt tttcgtcagc aaacgataca aagattcttt
    51721 gccaaattct tctctgctga attgcatcat ttcttgtgga agataatgaa caaaataaat
    51781 ataaggctga ttgttcaaat aatataagcg cttaaaacaa acagcttgct caccgaaaaa
    51841 ggaaaacaaa ggatcttcct tctttatatg gaccaattgc aaatccagcg tttctttccg
    51901 gacttcatat cgggagttct ccaagatttg cgtaaagctt gccgctttgg ataatcgatt
    51961 gtaaggacga ttgcttagaa ccgtcgtccc acgaccactt ttctttgcga catattcatc
    52021 tgatgccagc agttcgattg cctttcggac agtgattttg ctgacttgaa acagttcttc
    52081 taattcactt tctgtcggca acatcgaacc gactggatat tttcctgaga aaatatcttc
    52141 tttaatttta tctgcgactt ccaagtatag tacacgtgtc cttttcataa cgtcaccctt
    52201 tgctttacta ttactaaaag aatagcacat aaaaaataaa aaaagaacac aaatcacatg
    52261 tgagaagtgt gttctttttc attcagctgc tttaattaca agtttacgcc atttgaggca
    52321 attacttctt tataccaatc aaaagatttt ttctttgatc gattcagtgt cccatttcct
    52381 tcatcgtcta gatccacata gataaagccg taacgtttac tcatttctcc tgttgatgca
    52441 ctgacaagat cgatacatcc ccaaggcgta tagcccatca aatctacacc gtctgctact
    52501 gcattagcca tcgcttcgat atgacgacgt aaataatcga tccgataatc atcaataatc
    52561 gtgccatcct cttccatatg atcaatggct ccaagaccgt tttcaacaat gaataatggt
    52621 ttctggtaac gattgtataa ttcattcaat gcgattctca gcccttctgg atcgatctgc
    52681 cagccccatt cacttgcctc aagaaacgga ttttttactc ctcccagcaa gttcccttct
    52741 gaagttgctg cttgtggatc agtctcattg attgccgtag acatataata actaaatccg
    52801 atgtagtcca ctggatattc ttttagcaac tggaagtctt tttccgtttg ctctaatttt
    52861 tccggatcga ctccatattt tttatgggta cgcgctgtgt atgcagggta ttctccacgt
    52921 acttggacat ccgtgcagaa aaaattgaat tcttggtttt ggatcattgt tgctgcctga
    52981 ttgactggat tggcatcgat gctgtaagtt gttgcataga taatcatgca gccgatttgg
    53041 atatccgaac gcaattcgtg gccgattttt acggctttgc tacttgcaac gaattgatta
    53101 tgccatgctt ggaaaatatt ttggtactct cctgctccat tgctttttac taatccttga
    53161 cttaaagctg gaaagtgaaa ggccgaattg atttcattga aagtcatcca atatttgact
    53221 ttactgctgt atctctctaa tactgtctga gcaaatcgtt catagaagtc gatcagtttt
    53281 cgatttttcc agccaccgta ttcttttgcc aaatgaagag gcatttcgta atgagaaatc
    53341 gtgatcacag gttcaatatc atattttaag cactcatcaa tcagctgatc ataaaattct
    53401 aatcccgctt cgttaggagt accttcgtct ccatttggaa aaattcgtgt ccaggcaatc
    53461 gaaaaacgat aacatttgaa tcccatttcc gcaaacaagg caagatcttc tttgaaacga
    53521 tcgtaatgat cgattcctcg atgattcggg taaatatatt tttcttgatc aatcgtccag
    53581 tcaaattctt cactgccgat aatcgcaaag cgctgcttac ctccaggcat agcatctgca
    53641 acggatagtc cttttccgtc tcgatcataa gccccttcta attgatttgg cagcagttgc
    53701 tccaccccat aaaaattttt ctgaaaaacg tgattgcatc ctgatttcct ccttatttta
    53761 caattgccag aacatcttcg tttgcaataa tctctttttg gtctaattca agtatatcgg
    53821 caaatgcatc agtattcgta atgacaactg gagtgattac gctatagcca gctgctctaa
    53881 ttgcttcgat gtcaaaacga atcaataaat ctcctgcttt gacgagatcc ccttgtttga
    53941 ctaagatctc aaagccattc ccatttaatt ctactgtatc catcccgata tgaatcaaaa
    54001 tctccgctcc gtcagtcgtt gtgatcccta cagcatgtcc agttggaaaa atagtcgtga
    54061 tctctccgtc tgcaggtgca actagttctc ctacttctgg atcaatagcg ataccttttc
    54121 ccatcacgcc agaagaaaaa acttggtctg gtacttcact caatggcagt actttacccg
    54181 ttaaaggact ttttaaggta tgccgtgctg aagtaatcgg ttctcctgca ttcgcatgtt
    54241 tgttttctaa ttgattgtct tgtgtttgtt cgtcaaatcc taaaatcaat gtacctacga
    54301 atgcaagaac aaaagcaatt cctgtagcta ttactgctac tgtcacattt gactctacac
    54361 catccaccgt attaatgaaa gtcggaatcg tcaataagct gaccaaactg ctggagaaag
    54421 cttttactcc ggaaaatccg ataatagctc caccaatacc tccagaaata caagcggcaa
    54481 tgaatggttt cttcaaaggt aatgtcacac catacacagt tggttcagtg attccaaata
    54541 acgaagtcac tgtagaagaa atccctaatg ctcgaagttt cgtatctttt gttcgcaatg
    54601 ctacagctaa agcagcccca ccttgtgcca agatggctgg aagaagcatt ggcatcaata
    54661 catcaaaccc atattgttcg atattcaaaa acatgatcgg tacgaatccc caatgcatgc
    54721 cgaacatcac gaatacttgc cataaacttc ccatgattag acctgctacg attggactaa
    54781 atccataaat cgagttgaag agaccgccta aagcattacc ggcaaccgtc ccgattggtc
    54841 ctaaagcaag aaatgttaat ggaaccatca ctactaaaac gacaagcgga actaaaatca
    54901 tttgcagaaa ttgcggaatc acttttttta cgaatggctc taatttactt tgaacccata
    54961 cggctagaat gattggtatg acagaagaag tataaccaga tggtgaaaga atcactggga
    55021 tcccaaagaa actgatagct tctcccgcac cagcaactgc tgtgatagct ggatacacaa
    55081 gtgccatagc aattgctact gctaaaaact ggttggcttt gaacttttta gctgctgtaa
    55141 aagcaagcat gacaggtaag aaggtaaaga caccatctgc tgcagcaaac aatatttgat
    55201 aagtaccgct atcagctgtc agccagccca tcaccgttgc caatgataag aatcctttca
    55261 ataccccggc ggctgccatc gcccctagaa aaggtgtaaa tatactagag ataatatcga
    55321 tcagacgatt caaaatattt ttggactctt cttttccatc ctccgaaact gcaagacctt
    55381 cctcatccac gatcgattga taaacatctg ctacattact gccaatcact acttgatact
    55441 gaccgccact ttgtacgact tggatcacgt ccgggtcttc ttttaacttt tgcgtatctg
    55501 ctaaagattc atcttttaat ctgaatcgca accgcgtagc acaatgtact aaacttttga
    55561 cattcttttg tcctcctacc gcttcccata cacgacgacc aactgcttga ttatccattg
    55621 ttttttcctt ctttcttttt tatttaaaaa ataaaaaccc agattgaaaa aaacagtcac
    55681 aaacattcgt gaacctattt tttcaatcca ggttttgcct gccatacagt aacaatcctt
    55741 atgattggat gctttttttc tcacttacta ctctttgaat atgaatcgtt aaataaatct
    55801 gctctgcgat ccccatttcc tgtttttgcg tttgtcgaag atactcattg atccgttcga
    55861 cacaatgaaa cgcttttgga tactttcctt gaactaatgc gtagagaaat tcatcttgct
    55921 cgtcagatcc ttcccctttg agataacgct gggcaaaaaa ctgcagatga gtaatgatac
    55981 gctgataatt caaagattct tcatcgaaca cgatgccgaa gaatttactg atgatactca
    56041 gtatatctct taccattgtg gtagcggtca tcgtcacttg catgtcttcg tttccttgtt
    56101 ggctattcac aaaatgaaag gcaataaaac cggcttcatc ctcagaaagt tccacacccg
    56161 tcttttgttt cacgaacaaa agagcctctt tcgctgcttg atattctttc ggataaaatt
    56221 tttttgtttc aaataataat ggattaggca gttggatatt ttcttttgct cgtgtgatcg
    56281 cataatgaag atgatcggtc aatgtcagat aaatattagg cgaaaattca acttttagtt
    56341 tatcttcagc cagttcgact acttgtttga cccattcgat ctcctctgcc ggaatatcag
    56401 ccatcagctg ctggaattgg tttttagcaa aagaatctct taaaacaaat tctttttgga
    56461 ccgcttctgg cgaaatctcg tccccaatct ttttttgaaa agctagtcct tttcccatac
    56521 aaacgacttc ttccccgaat ccatttttcg aaatgacgac attgttattc aaaattttat
    56581 caattttcat tttgcttcgc cctctcaaga aaaataaaaa aacctagaat tcgcctactg
    56641 tttcttagct gaattctggt tttgcctgcc gtacagtaac aatccatttc ttattcaaaa
    56701 tcatcatacc atcgaatttt catttatgca agccttttca tataaaaaat aaaaaaactg
    56761 gaacacaagt ctatctagaa aacaaccaaa tcagagatgc acctctttgc gtttctcttt
    56821 ttttcagcaa attggttttt ccttatcgat tcatatcctt tggctgtttt taatctgtaa
    56881 cgacttatgt cccagtatcc taaaatgttt ttgtctctag attgaaagca gatctcatat
    56941 catctgtcgc atcttttatc gatgtaacac tttccagttc tccttgtaca actagtcgcg
    57001 tgacggcgtc catgtcgcca caagtaatta gtgtgacaat ttcttttccc tcgacttcat
    57061 tcagtaattg ggtatctgta ggagcaactt tttctttaaa aaatgttttg tatgtataca
    57121 cattttctaa atctgtcaaa tagatcttgg cacccatctc taaattttct agcggtgtaa
    57181 acagcaattc agggttcgtt gcacgatggc ttgctaaggc ataatttcct tttcccattt
    57241 cttgatctgg agataatgtt cctgctccgt aaagtagtga cgtattatcc aagcctttga
    57301 aaaccggtaa attgattgaa actgaaggga ctgcgatact agcaatcact ggtagatctg
    57361 ttcctctcat ttgcgacctc atgacccctt ctgtcgtcat tggttctacc gcatcaaagt
    57421 caaaactgac atctttatcg ttattctttt tcagatcttc ttttgttaca ttcgctatag
    57481 catatttatc tcctgtctct ctgacaaaat aatccttgat ctgttcattg aaaatcaaag
    57541 ctagtcctac taataacaag aggaacaaga ggatattgac tagccatctt tttgcttttc
    57601 cttttttttt cattttctct ccctccagac tcattttact gtttttcttt tgctctttcc
    57661 aacagccgaa tgacacattt ctacaactct gtgtattttt ttcgtacacc tgcgcttttt
    57721 tctacaggat atttcaaatt atttttgatc aatttttctt caatgatctc atctagatcc
    57781 aggtgaaggt tgtctgccat catgtatgaa tagatcaata catctaccag ttcttctttg
    57841 attcgttcta attgtgtttc ttttacttct tccggcgttt tccattgaaa aagttctaac
    57901 aattcagatg cttccaaaga aatagagata gccaaatctt tttcgttatg aaattgtcgc
    57961 caattacgtt catctctaaa ctggttgact ttttccatac tattcatttg cttgccttct
    58021 tccaatcact tcttacttga ttaatcatac cattttttta cataaaaaat agatgatgct
    58081 tttaaaaaaa ctcctgtttt cttttattta aaataaatgt catagaaaat caataattta
    58141 ggtagccacc atgtgtatgc agtagcgtgt cttcttccct tgaggctttt tgtttttcga
    58201 tacgttcttc ttcccaaaca gacaacgcta ctagaatctc tcttaatgat tttccttctt
    58261 cggtgagcga gtactctact ttaggcggga cttggttata ggcttttcga tggatacttt
    58321 catcttgttc taactctctt aactgctggg tcagcatttt ttttgtgatc gtagggattt
    58381 gcttgcttct ccacgcctaa aataagcgat attgctcttc cctttatctg gtgtaagcaa
    58441 agcagttagc attttgacga tcgtgggttc cgcttttcac ttcttatcta cttagtatca
    58501 tgaagtatac tttcgtacta atactaagta gcggaagtgt caactacttt gtttaactac
    58561 atagcaaaag aaacagaagg gtgacaacgg tcatcacgct tctgtttctt ttgaatatgc
    58621 tgtgttgttg tctctatctc cgtgtagact ttcctcaaac ttatcctttg acagtaagtc
    58681 agtaggctga tttgctcttt ctagatgcca gatacatttc tacaaaaaaa tagtgattct
    58741 gttttgaaaa ttcgattttt agttctgcat tttcgtaaac atccaacatt tttttcgcac
    58801 tgtatagacc taacccgcca ttttttttag aggtttttcc tcttttgacc atctccgata
    58861 gcgtcaaatt cgttggattt gccttgttcg ctatactaaa acgtgatccc tctttttctt
    58921 ccagcagatt gatcgtaatc ggactctgat cggctacacg atgatcgaat gcgttagaaa
    58981 taaaaataga taaacagcga ataaaaacaa ccagatcaat cgggattgtt ttcacttcga
    59041 caggaataac tacttggatc gggattttct ccgattctgc tttttctaga aattttaaaa
    59101 agacacccct gacagctggg gatacaatat ttgttagctg ttgtatttgc ggtttttcaa
    59161 aaatagctgc agaatgatct tctaatattt ctaaaaattc caacgtttct tttttatttt
    59221 cctgaagagt aatttttaat gaaaacagta gacttttata atcatgtttg aattcctcta
    59281 gatgatggta atattcttca tttatttgat acagttgaag cgattcctga tgttgtcttt
    59341 cttttacttg gaaactgatc aacataatgg aaaaaaacaa agaaaaaaaa ctgatgacta
    59401 tcgacaaaag agatagaagc agataattag atgagcggaa gtgaacactt cccaatacca
    59461 agtaaatcac gccccagctt aataaaatac aaatacctcc aaaatagtgt tcagttggat
    59521 aagtaaaaag caagttgaga aatctcgtct gtttgtctat actttttact aatagataga
    59581 aaataaatgc gataagtagt gcaaaaaaac ttgctgttcc aagatgtctc tttcctaaat
    59641 aatccatcag atcaaaaacc agaaaaaagc tgagtccagt gatagcaaaa aaaagattac
    59701 tgatcaaaca gctgataaac aggtttttcg tagtaaaata aaaaagaaac atcaataata
    59761 agccaactag gtaaggaaag ctcactttag ggataggaag taaaagtgtc accaccatca
    59821 aagctataca accaaagctg ttttttacta gcagttgttt cgaaaagaat aaatagatac
    59881 ctaaatctaa aatcaaagta aaggtcaaat aaagtgtcac tgttgcgacc atcttattcc
    59941 tccattgttt tgtttcaaaa tgtacacata tttttttaat aggagcctgc atatgaaaaa
    60001 gtattttatc atagaagata accagtttca cagcgaatgg atcatcagag aattggtcaa
    60061 tcatcatatc gagaatatcg tgcagctcac ttctatctct caaatcaatc agttgatcca
    60121 gttgggcact attgataatc acgatgtttt ctttatcgat atcgacttga agcaatcact
    60181 cacaggtatt gatgtcgccg aacaaatacg taaaaaaaat gcacatagta atattgtttt
    60241 tttcaccgct tatacgaatt ttgcagtaga gatcatcaat cggaaaatcc ttccgattgg
    60301 atatatcaga aaagacagga atttatctga acaaattcgt caagtgctag aacagatcaa
    60361 ttggaaagaa cagcagatcg taaatgtcga taaaatcttt attgatcaaa aaggtggaag
    60421 tctcgtcctg tatcctcggg aaattttata tattgaaagt atcaaaggtt tgaaaaacca
    60481 agttatggtc aaaacaacta cagaagataa attagtgaat acttctttaa aacaattgaa
    60541 gacagagtta gaaaagtatg cttacttttt acttttaaaa tcttactgta tcaacttatc
    60601 gcaattgcag aaaattgatt ctgctcatta tgtgttagaa tttttcaacg gtgactcctt
    60661 actcgttggt cgaaagatct ttgaaaagac aaaggaacgt ttccatgact tccaaaaaac
    60721 tgcttcgagt tgatgaataa gagctgagac gtaagcatag cagatttcag ggagtgggcc
    60781 ttgaatcggc tccactccct gaaatcgttg atatgatttt acgcttcctc ttctgtagga
    60841 gcatctgtta acgaccatgt gaatgttgaa acgtagttgt ctacagcata attggataca
    60901 ggaacaaata attccactga ttgtctccct tgttccacat tctctcctaa tgaataaatc
    60961 catgtgccaa tcccttcatt tgctcctgca gttactagat ctactgcctg atctgctggg
    61021 atttcgattc cttcagcgcc agttccagaa acggtagatg gcattccact gctattagtt
    61081 gcgctatttc gaatcacggc gtttttgaat ttcacctttg aaccagttag tttattcgta
    61141 tcagatgttg taccaataaa ttctgttcgt ttgactgata atttccaacc ggcataattt
    61201 ccgcttttat ccgttacttg tacgaaattc tgtacttgtg tttgtgctcc agtactgtcc
    61261 gttccgcttt gtaatgcagc atatactgtt tttgctgtac ctaacttgat tgttccaaaa
    61321 tcaagatctg gagcttggtc aatcgtcagt aatccggatg ttccctgatt tggttcgtca
    61381 ggtgtaaaag gagtatcagg atcatttgga tcaacaggag gaactggaac aacgtcttca
    61441 ggatttgcag catctggttt ggtcacagaa atcgttcctt ctgtagtggc tgagctctta
    61501 ttctcagcaa aaacactttg tgtacataat aatagacttc cagctagaac ggtacttaat
    61561 gcaattgttt ttttcatttc tttttccttc tttcttttaa aataaataat ccagcaaata
    61621 agccaataag aatgactcca acaagtatca gccatcgcca gtcaaatgtt tctggctgat
    61681 tttttaaatc aatatccttt tcattcaatt ggtccgctgc atcatcagtt atttcaaaat
    61741 cggtatggag attccagcgt ttttctttcc aagcaccctt cacaaccgct tgataatgtc
    61801 ccggagctaa tttttttcct tctaatggga caagtaaatc aaaatttgta ttgggtgcta
    61861 catctatttt ttcttttttt attgaaaggt aaggctcttt tgtttctttt ttgtagattt
    61921 caaccgatgt agtcaagccg aacatataag aagcatttgg attttgaaga ttcgccttaa
    61981 tcacatttct tccatcgctt tgatccgcat aagctttttt taatttcaaa tctggggaag
    62041 actctttttc gctatttcaa atcaagattg ctttcacttg agaaaactga ttgacgacac
    62101 caactgtact tttgttagaa acgttctctt ttttttcttg aaacgtgatc cctcctgcga
    62161 ccacaccagc cagctctttt tctggcgctt tcagtgaaag tgtctcagtg accgcacttt
    62221 gaggaggaac agttatttct cccactgttt ttgctaagtt tttcatatca taacttaatg
    62281 tttcatcaaa tttacttttt gattctccat attcgatcac accgtttgaa ttcgtcgtag
    62341 cggagtggat caatgtctca actgttactg atttatctgt tcggttaaag accgtgtagg
    62401 ggatttcctg tgttttccca ggctccatcc ttacatttat atatgttttg ttcttatcaa
    62461 tctgattagc cggtaattca aaatctaccg caaaattgaa ttcggaggca tggacaggtg
    62521 tctgccaaag gatgaatcca aggagaagga tcataagtaa agagcagttg tttcgatgac
    62581 ttttattcca tattcgattc atcaatcatc ctccttgatc tttattctgt cggcgcatct
    62641 gctaatgtcc atgttatctc tgctgagtaa ttaccagcta tctgattttc tttggttaag
    62701 acaagtttaa acgcccgacc gttatttaaa tattctgaat aatctgcttg cacattcgtt
    62761 gaattttgtc ctctgacaat ttccagttct tcttcattca ctaattgata ctcctctcct
    62821 ttcttgaaat ataggacatc ctgcatagaa aatcctgcat catttgtgaa aggctgagat
    62881 aattttatcg ttaagatcca cggatttttc gtttctcgct gatcataaat ccataatttt
    62941 tcctgtatat ccatttggcc aaatgcttct tgtgtcaaac cagttgcttt gatctgacca
    63001 aaatcgagtt tggaaggggc aagaaatgag agaccacctt ctactacgat cttcgtagca
    63061 ggtgcaaaag gcggggcatc atgataaaca actggcgtat cacttggatt gtgattccca
    63121 atttcatcgt tggtacttgg cttgttctgc acacccttag cagtagcttc tcctgcttgg
    63181 tcgtttaata caatttctaa tacatctcct gcaaaaagtt gattttctgg gatagaaaga
    63241 acaaattccc ctgtatcatc aatagttgtt tctgacaaaa cagcttgatt ttttttcaat
    63301 gaaaccaagg ctcctgcttc acctgtacct gtgatcgtgc gagaagctgc attgatcaat
    63361 gtggtactta aattagcagg ttcaggagga atcacatcga ctgtcgtttt ggcagagtcc
    63421 agttctgtca cttcttggcc acctcttact tttttagcag caacaacttg cacttgatcc
    63481 tccgttttta atctctcatc attgggtact tgaacttcga acaagccgcc ttgttcgcct
    63541 tctccccatg ggttatatcc tgccccttca ttttcatctg tttgtccttg cgtgagagca
    63601 gtaatttcta cagatgattt cgtcgtatct gcgtaagtaa tacgtaaagt cacttctact
    63661 tcgcctgtcc aagcatctct tggctttgcc tcgacacctt cgggaacaac gacatgccca
    63721 tagatttttt tatctgcatt cgttggcact cttaaggatt caatcatcgg gagctgatta
    63781 ttggcactga ttcgattata atttttcatt ccggcacctg tctgagcaaa ttcatttgtt
    63841 aaacctgcac tagggcttga tacgaaattg cctaagtcgg tcccattgat cgataagtct
    63901 gtcatatcag aaaaataatc cgggtctttt tcaaagttat cgaatgttgc gcctgttttt
    63961 cgccataaag cgatttctga atgggcgatc tttaaagaag aatctccatt tccttggaat
    64021 acccagccgc ctatgccatt ggtctcgctt ccaatccgtt tgttttggaa atcaaaatag
    64081 gtaggagatt ctaagtccac attcaacaca tccccactga tgatgccggt cttatcttcc
    64141 cctgttttcc cgaccatgac taaataagtt ttttcacctg ccgtaaaatt taaatcaata
    64201 tctccagttg tatctacagt tgctccggaa tctgcgcgta tatcgatatt tgatttttct
    64261 ccagtgactg taaaatcagc tgaacctgga ctttctagaa aaccagaagc gtcataaaat
    64321 tggatcgctt gatttccttg attgagtctg ttcacgtaag atttgccatc acctgtattt
    64381 cttaccctca atgagcctcc attggttact gtcagctttt gcgtaccatt ctcaaaacga
    64441 actgcgctgg aacgcccact ctgcttatcg atgtcgataa ctgatccggc tccttctacg
    64501 atcaaagaaa ggccttttcc tacaaaacgc atcccattgt tcccgggttg atcgtcatct
    64561 ccagccattt tgaccgagat ttgtccacca cttgatacat ggatttgact agaatctcct
    64621 tgcaagcgaa atccgcccgt agttgctgta ttgatggtga ttttcccacc attcgataca
    64681 gtgactttcg atcctgaacc tctcatttgg atgattccgc gattgttgtc attggtaata
    64741 ttgatctttg tatttaccga aaattcgctt cccgaaccac tgacttcaaa catgacatta
    64801 ccaggtagcg tatcgactgc ctctccttca tcgctaacgc catctgcccg atcataatca
    64861 agagagacct ttgaaccgct taagatatgg attcctggcg ctgtttttcc actaccagtc
    64921 tcatctcctg aatctagaaa accgtagaat ttccctagat acggtccata aagttgacta
    64981 ccattgatca cattcaattg atagcttcta caaaaatagg ctttacccgg agcggcattc
    65041 agtccttcct tcactggttg gacatccaac acaccggaaa caccatctaa tgtgacagtt
    65101 gcacgaggag cgtaaatcag tcctaatttg gacccatcat acaaatcaag tgtcccggta
    65161 aattttattt ctcccgtaaa atgatatctg cttccccaaa aatcggtcat ataagcaatg
    65221 ataatggcat ttccatcgat atttggtgtc aatccacctg gatgtgcagc tgttccttta
    65281 aaattctgga actcgatcga ctgaggtatc tcattgatat aaatatggtg tgtgccgaaa
    65341 ttgtgagcga atccaccaaa atcaataacg atatctcgtt tgatctctaa ttccctgtct
    65401 aaagtcaaag gctgaatcag tcggatctta gttactgatt cgtctgcgag aagctgcgcc
    65461 agttcttctg ttgtggcagc ttcgctgtac ccatccgctg aaacagctct tgccagtgcc
    65521 gcttcactgc tatcagacgt ttgttcagct acttctgagg tctcacttgt cttattttct
    65581 tcttgccttt tattagtaga agcctcactt tgagattctg tagtttcaga agtaatcgat
    65641 tcttctgaaa aagtagtctt gtcaaaactt tgattctctg attgcgttga ttcgtttgtt
    65701 ttctctacat cggcaaaaac ggatgtcacc atcaagagag ggaagacaaa caaaatcgac
    65761 gcaatcagct tttttacaca ttgattcatt ctccaccatc ctctctgacg ttttgacaat
    65821 tttattttat cgttttaatc tatttccaat tgtactttca tatttaaaat atgtattttt
    65881 atggaaaaat ttttctatta ggtgtaaatt tcttttttgt acaaaaaaaa ggctgtgaca
    65941 aaagtcttgt cacagtccct tatcccgaac aagcggtgtt ctcccgccaa ctcctcggtc
    66001 acaacctcca ttttaacaat aaacctgtaa atgtgagggc aatactttga ttgtcacagg
    66061 taatttatcc ccttcatctc catcaacatt cgtcgtcaat tcttcctcat cagctaatga
    66121 cacaactaat tgctcacaag taaggtaggc tagatttttc gtagattcat ctactccttt
    66181 taataggtct ggcaatgttt taagcgtatc gatcaaagaa gaatctttta aataaaccaa
    66241 atggagctta ccatcatcca cttttgcatt tggaagtaat gtttcaaaac cgccgactga
    66301 gttagttagt cctatcagca aggtactgct ctcaatttct tccttctttc catcaacttc
    66361 tacgtgaaac gagtaggatt gtgtggaagc taactgtttg attcctgaca tgaaataagc
    66421 tagttttcca aatttcgtct tcttctccgc atctacatca ttgattgatt cagggataga
    66481 gccaattgcg acgacattca taaaatagtc gtcatttatt tttccaatat ccaatggctt
    66541 gactgattca atcgaaaaat gattgatcgc ttcctcaggt tctagcggta tccctaatgc
    66601 tcgagccaag tcattcaccg tacccaatgg gaaaaatccg aaatttggcc gatgttcttg
    66661 ttcagcgatt ccactgattc cttcgttgac tgttccatct ccgcccatga caaacacact
    66721 atgataccct tccgtagccg cttcgcgagt aaagtttttt gcatcccctg ctttttttgt
    66781 atgcaagacg accacttcat caaacaccga ttccaatttt gctatcgcta attgctcaaa
    66841 ttcctttgct tgttcaccgc ctgaactggg gtttaccact aataaaactt ttttcatcac
    66901 tttctttccc ttctgattga tttctttaat ataacgatag cataaagaaa aaaattaggc
    66961 tgtgatgaag tactgttcat gaaacattca tttatttttt gacaagataa aacatccata
    67021 ctatgaaact gaggaaaatt aaaacaatga tataggaaat aaaagaaaat gataggcatt
    67081 gtatacctaa taaagtaaaa atcttaggtg gcagataaaa acacaaaaat agcaagagaa
    67141 tgtaatagcg tttcttccac tttttgttat cagaccttat agcgatcaga ggaataccaa
    67201 atgcataact gaaaaactct tgatgtactg ttaatatgtc acggccttga ataataaatg
    67261 cgacaatccc cgctataatt agtgtgccta acaccaaaac ggaaaattga tgaatatatt
    67321 ttatatacaa atgatccatt gtacttcctg cctttttatt tgtgagaaaa acaaatccgt
    67381 atacgtgaat aacttctaga aataaaagaa atttgcaaaa ccactccatt tatctacttc
    67441 tacgtgcatt ttttgatatt gttttcttta tttaagatta tacagaaaaa tatctagggt
    67501 cgctggattc taagtaaaaa aaatttttaa aataaaaaga actaaatcaa gtagtaa
//
LOCUS       DASHRQ010000006        65952 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.006, whole genome shotgun sequence.
ACCESSION   DASHRQ010000006 DASHRQ010000000
VERSION     DASHRQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.006"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="U2M88_000424"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="U2M88_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEM8372834.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="U2M88_000425"
     CDS             852..1040
                     /locus_tag="U2M88_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEM8372835.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="U2M88_000426"
     CDS             1053..1610
                     /locus_tag="U2M88_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEM8372836.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="U2M88_000427"
     CDS             1642..1884
                     /locus_tag="U2M88_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEM8372837.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            2225..3439
                     /locus_tag="U2M88_000428"
     CDS             2225..3439
                     /locus_tag="U2M88_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEM8372838.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            3470..4897
                     /locus_tag="U2M88_000429"
     CDS             3470..4897
                     /locus_tag="U2M88_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8372839.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            4920..5846
                     /gene="glsA"
                     /locus_tag="U2M88_000430"
     CDS             4920..5846
                     /gene="glsA"
                     /locus_tag="U2M88_000430"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEM8372840.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6054..7514
                     /locus_tag="U2M88_000431"
     CDS             6054..7514
                     /locus_tag="U2M88_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372841.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            7735..9498
                     /locus_tag="U2M88_000432"
     CDS             7735..9498
                     /locus_tag="U2M88_000432"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEM8372842.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            9582..10040
                     /locus_tag="U2M88_000433"
     CDS             9582..10040
                     /locus_tag="U2M88_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEM8372843.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            10149..10832
                     /locus_tag="U2M88_000434"
     CDS             10149..10832
                     /locus_tag="U2M88_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEM8372844.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            10979..11593
                     /locus_tag="U2M88_000435"
     CDS             10979..11593
                     /locus_tag="U2M88_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEM8372845.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            11609..11815
                     /locus_tag="U2M88_000436"
     CDS             11609..11815
                     /locus_tag="U2M88_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372846.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            12354..12911
                     /locus_tag="U2M88_000437"
                     /pseudo
     CDS             12354..12911
                     /locus_tag="U2M88_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            12969..15008
                     /locus_tag="U2M88_000438"
     CDS             12969..15008
                     /locus_tag="U2M88_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEM8372847.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(15117..16268)
                     /locus_tag="U2M88_000439"
     CDS             complement(15117..16268)
                     /locus_tag="U2M88_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEM8372848.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(16422..16952)
                     /locus_tag="U2M88_000440"
     CDS             complement(16422..16952)
                     /locus_tag="U2M88_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEM8372849.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(17051..17401)
                     /locus_tag="U2M88_000441"
     CDS             complement(17051..17401)
                     /locus_tag="U2M88_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEM8372850.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(17558..19087)
                     /gene="ahpF"
                     /locus_tag="U2M88_000442"
     CDS             complement(17558..19087)
                     /gene="ahpF"
                     /locus_tag="U2M88_000442"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEM8372851.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(19097..19660)
                     /gene="ahpC"
                     /locus_tag="U2M88_000443"
     CDS             complement(19097..19660)
                     /gene="ahpC"
                     /locus_tag="U2M88_000443"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEM8372852.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(19804..20466)
                     /locus_tag="U2M88_000444"
     CDS             complement(19804..20466)
                     /locus_tag="U2M88_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM8372853.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            20650..22296
                     /locus_tag="U2M88_000445"
     CDS             20650..22296
                     /locus_tag="U2M88_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEM8372854.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            22287..23369
                     /gene="uxuA"
                     /locus_tag="U2M88_000446"
     CDS             22287..23369
                     /gene="uxuA"
                     /locus_tag="U2M88_000446"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEM8372855.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(23477..25030)
                     /locus_tag="U2M88_000447"
                     /pseudo
     CDS             complement(23477..25030)
                     /locus_tag="U2M88_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            25178..26466
                     /locus_tag="U2M88_000448"
                     /pseudo
     CDS             25178..26466
                     /locus_tag="U2M88_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(26592..27671)
                     /locus_tag="U2M88_000449"
     CDS             complement(26592..27671)
                     /locus_tag="U2M88_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEM8372856.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(27774..28214)
                     /locus_tag="U2M88_000450"
     CDS             complement(27774..28214)
                     /locus_tag="U2M88_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM8372857.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(28271..28681)
                     /locus_tag="U2M88_000451"
     CDS             complement(28271..28681)
                     /locus_tag="U2M88_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEM8372858.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(28861..29295)
                     /locus_tag="U2M88_000452"
     CDS             complement(28861..29295)
                     /locus_tag="U2M88_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEM8372859.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(29395..30243)
                     /locus_tag="U2M88_000453"
     CDS             complement(29395..30243)
                     /locus_tag="U2M88_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEM8372860.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            30632..32398
                     /locus_tag="U2M88_000454"
     CDS             30632..32398
                     /locus_tag="U2M88_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEM8372861.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            32500..33759
                     /locus_tag="U2M88_000455"
     CDS             32500..33759
                     /locus_tag="U2M88_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEM8372862.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            33906..35207
                     /locus_tag="U2M88_000456"
     CDS             33906..35207
                     /locus_tag="U2M88_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8372863.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            35210..36061
                     /locus_tag="U2M88_000457"
     CDS             35210..36061
                     /locus_tag="U2M88_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8372864.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            36074..36889
                     /locus_tag="U2M88_000458"
     CDS             36074..36889
                     /locus_tag="U2M88_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEM8372865.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            37040..38206
                     /locus_tag="U2M88_000459"
     CDS             37040..38206
                     /locus_tag="U2M88_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM8372866.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPTQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            38436..39083
                     /locus_tag="U2M88_000460"
     CDS             38436..39083
                     /locus_tag="U2M88_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEM8372867.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            39167..40111
                     /locus_tag="U2M88_000461"
     CDS             39167..40111
                     /locus_tag="U2M88_000461"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEM8372868.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            40246..41697
                     /locus_tag="U2M88_000462"
     CDS             40246..41697
                     /locus_tag="U2M88_000462"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEM8372869.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            41718..42554
                     /locus_tag="U2M88_000463"
     CDS             41718..42554
                     /locus_tag="U2M88_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEM8372870.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            42554..43213
                     /locus_tag="U2M88_000464"
     CDS             42554..43213
                     /locus_tag="U2M88_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEM8372871.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            43339..44028
                     /locus_tag="U2M88_000465"
     CDS             43339..44028
                     /locus_tag="U2M88_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372872.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            44034..44990
                     /locus_tag="U2M88_000466"
     CDS             44034..44990
                     /locus_tag="U2M88_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEM8372873.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            45034..45546
                     /locus_tag="U2M88_000467"
     CDS             45034..45546
                     /locus_tag="U2M88_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8372874.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(45802..46350)
                     /locus_tag="U2M88_000468"
     CDS             complement(45802..46350)
                     /locus_tag="U2M88_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM8372875.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(46439..47620)
                     /locus_tag="U2M88_000469"
     CDS             complement(46439..47620)
                     /locus_tag="U2M88_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEM8372876.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(47856..49364)
                     /locus_tag="U2M88_000470"
     CDS             complement(47856..49364)
                     /locus_tag="U2M88_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEM8372877.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            49523..49714
                     /locus_tag="U2M88_000471"
     CDS             49523..49714
                     /locus_tag="U2M88_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372878.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            49917..50834
                     /locus_tag="U2M88_000472"
     CDS             49917..50834
                     /locus_tag="U2M88_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEM8372879.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            50827..51900
                     /locus_tag="U2M88_000473"
     CDS             50827..51900
                     /locus_tag="U2M88_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372880.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            52048..52566
                     /locus_tag="U2M88_000474"
     CDS             52048..52566
                     /locus_tag="U2M88_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372881.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            52547..52939
                     /locus_tag="U2M88_000475"
     CDS             52547..52939
                     /locus_tag="U2M88_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEM8372882.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            53015..53950
                     /locus_tag="U2M88_000476"
     CDS             53015..53950
                     /locus_tag="U2M88_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEM8372883.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            54083..54862
                     /locus_tag="U2M88_000477"
     CDS             54083..54862
                     /locus_tag="U2M88_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEM8372884.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            54855..55172
                     /locus_tag="U2M88_000478"
     CDS             54855..55172
                     /locus_tag="U2M88_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEM8372885.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            55416..57026
                     /locus_tag="U2M88_000479"
     CDS             55416..57026
                     /locus_tag="U2M88_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEM8372886.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            57183..58031
                     /locus_tag="U2M88_000480"
     CDS             57183..58031
                     /locus_tag="U2M88_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEM8372887.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            58262..58741
                     /locus_tag="U2M88_000481"
     CDS             58262..58741
                     /locus_tag="U2M88_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372888.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            58774..61002
                     /locus_tag="U2M88_000482"
     CDS             58774..61002
                     /locus_tag="U2M88_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM8372889.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAATGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            61131..62774
                     /locus_tag="U2M88_000483"
     CDS             61131..62774
                     /locus_tag="U2M88_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEM8372890.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(62847..63392)
                     /locus_tag="U2M88_000484"
     CDS             complement(62847..63392)
                     /locus_tag="U2M88_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEM8372891.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(63415..63900)
                     /locus_tag="U2M88_000485"
     CDS             complement(63415..63900)
                     /locus_tag="U2M88_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEM8372892.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(63890..64702)
                     /locus_tag="U2M88_000486"
     CDS             complement(63890..64702)
                     /locus_tag="U2M88_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEM8372893.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(64841..>65952)
                     /locus_tag="U2M88_000487"
     CDS             complement(64841..>65952)
                     /locus_tag="U2M88_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEM8372894.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        22009 a        11767 c        12894 g        19282 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc cttatgaaaa aatatcttta aaaataaaaa aagacgaaaa taaacggttt
     2041 tctataaaaa gaagaccgtt tattttttgt ctttatttgt gtgaaaaacg aaaaagagca
     2101 caacacatca agcttttatt aattgtctgt cttttataaa tgcaagtatt aattcaaaaa
     2161 aacagaagaa aaaaacaact acaccttctt ttatactcgt aatcgtagaa aggaggcgaa
     2221 aaaaatgaat acattaccta tcaatacagg agatacagca ttcatgattt tatgtacagc
     2281 aatggtttgc ctgatgactc ctggtctagc ttttttttac ggcggattag cacggaagaa
     2341 taatatacta acgatcatgt ctcaatcttt tctctccgtt gggataacaa cgattatgtg
     2401 ggtatttggt ggattcggat tggcctttgg acgggatatc ggcggtgtta ttggaaaccc
     2461 ggcagatttc tttttgatga gacatgttac aagcttcccc aacatgtttc atggtgcaac
     2521 gattccattt ttaatgttct ttctttacca actgatgttt atcgtgatta ccgttccttt
     2581 gatgacagga gcttttgcag ggagattgaa tttaaaaggc tatattatac ttgttatttt
     2641 ttggaatctg ctgatttatt tccctgtttg ccactggata tggggtggcg gatttttgga
     2701 tcagatggga tttagagatt tcgctggtgg agcagtcatc cacacgacag ctggattcgg
     2761 ctctttagca tgtatcctca cacttggtca aagaaagatt cctttaggag aaagaagtca
     2821 gcatagcagt cttatggcag ctgctatcgg tacaggactg ctttggtttg gctggttcgg
     2881 ttttaattcc ggtggtgcac ttcgagcaga tgaacaagca gtcaatgcat ttggttcaac
     2941 attcatcgct cttgcatttg cgatgattac ttggctgatc attgcaaaag taaaaggaaa
     3001 tggctttgat tttgtcgacg tactaacagg atccgttgct ggattggcag ccattacgcc
     3061 ttgtgccggt tatgtagaag caaaaagtgc catgctgatt gggatcattg cgggtatcgt
     3121 ctgtcatgct gctgttgatt ttcgtaaaaa gaaacaatgg gatgatgcac ttgatgtgtg
     3181 gggtgttcat ggcatgggcg gattcacagg tacgattctg atagggatct ttgcatctaa
     3241 cagccattta ctaacgaatc caagcagctg gtacttctta gggattcaag tgattggtgt
     3301 catcattact gccgtgtatg cctatgtgct gacaacagtg attttaaaat cggcgatgca
     3361 ctttacaacg atcactacta caaaagaaga acaagaagaa ggattggact ttttcttctt
     3421 tggcaacaaa ctcaattgaa acaaattcaa atagaaaaga ggaagattca tgcaacaaag
     3481 caaaacgaaa attcctgcaa aaaaattaac gttctttggc tttttggcta tgaccatctc
     3541 aatggtcgtt tcattatatg aatatcccac attcgcgacc tcaggcttca gcttagtatt
     3601 tttcttgtta ttaggcggtt tgctatggtt tatccctgtg gcattgtgcg cagcagagat
     3661 ggcaacggtg aaaggatggg aaaaaggcgg tgtctatact tgggtcagtc gcacattggg
     3721 aaaacgattc ggttttgccg caatcttttt ccaatggttt gagataacag ttggctactt
     3781 gaccatgctg tatttcttaa ctggtgcatt atcctatgca acgggtattg gtgctataca
     3841 aaacaataaa tatctgaaat tggcaatcct attgattatt ttctgggcta ttttactctc
     3901 acagttaaaa ggaaccaaat acacttcatt gattgctcgg atcggttttg tagctggtat
     3961 cttgttgcca gcattagtct tgtttggctt agggatccat tatgtcgctt caggcgctcc
     4021 actacaaacg atactgtcta tgaaaacatt gattcctgat ttttcgaaat tgcctacgct
     4081 ggttgttttt gtttccttta ttttagcata tatgggcgta gaaacatctg ccagtcatgc
     4141 aaatgaaatg gaaaacccta aaaaggatta tccattagca atgtttgtat tggttattct
     4201 ggcaatcatt ttagatactt ttggcggatt gaccgtcgct actacagtac ctcaacatgg
     4261 tttgtctctt aatacagggg tgattcaatc gttagaattc ctattaagac acattgatcc
     4321 aaatcttgca tggattgcga aaattcttgc gattcttgtt tgtttaggtg tcatcggaga
     4381 aattgcttct tgggtaacct ctccttcaaa agcacttcac gtagcagcag aagaaggatt
     4441 acttcctgaa tattttgcaa aagaaaacac acacggtgtt cctgctcatt tgatgatcgc
     4501 aaatgggatc gttgcaacaa tctgggcagc cgtctttaca ttaagcggcg gggggaataa
     4561 tctttcattt cttgcagcga tgtcattgac tgttgtaatc tacctgatgt tgtactttat
     4621 gtttttcgcg ggctatttga agctggtttt taaagatgac agtcttgaaa gagcctatca
     4681 ggttccaggc ggaaaagttg taaaagtaat cgttgcttgt ttaggtcttt tgacttcact
     4741 ttttgctttt tgtatctcat ttgttccacc tgctagtatc gcagctggac agcatgggat
     4801 ttatgaaacg attctgattg tttcttatct tatcgtctta gttattccat ttacgatcta
     4861 cgcatgccgt cataaattcc agcatgcaga aaaataaaaa atgaaagctg gttattcaaa
     4921 tggaagtaaa agaattagaa caattgattg gaaaaaattt ccactattat caaacaggac
     4981 aagtggcgga ttacattcca gcattagctg aagtggatcc caaacaatta ggtatggcga
     5041 tttatgattt ggatacaaaa caactgattg agggaggaga cgccgatgtg cgctttgcca
     5101 tcgaaagcat gtcaaaagtt cccgtattgt tattagcaat caaagacaac ggaatagaaa
     5161 aagtctttca aacaatcaac acggaaccaa ccggttttgc cttcaattct atcatgaata
     5221 tggaaatcaa tcatcgcaaa catccaatga atccttttgt taatgcagga gcgattgcaa
     5281 caacctcgtt gattgccggt aaagatgcag atgaaaaatt tgagcgaatc ttgtcattta
     5341 tgaaagagat ctgtgatgat cctaatatca cattgaatga agaaatctat cattctgaat
     5401 caagaacagg tgatatcaat cgctctcttg cttactatat gaaaggtaat caaatgatcg
     5461 aaggagacgt accggagata ctcgatgctt atttcagaca atgctccctc aatgtcaccg
     5521 ctacaggcat agcaaaatta gctgctgtgc tggcaaataa aggaattgct ccttggaatg
     5581 gcaaacaaat cattactgaa gaaagtgcta caatcgtcaa atcaattatg acaactgctg
     5641 gtttgtatga tgaatcagga gagttttccg tccatgttgg tgtacctgca aaaagcggtg
     5701 taggcggcgg gttgatggca gctgtgccaa atcgttacgg cataggtgtc ttcagcccgg
     5761 cactagatcc atttggtaac agcgcagctg gtatccaatt gttgaaagat gtagtcaaag
     5821 aactagacgc tgacatcttt gaataaccat tcttgaaaga ggctggggta aaagtccaaa
     5881 cggctcaaag ctacctacaa tgaggctgaa attgtgagct tgtttgactt ttgtcccagt
     5941 ttcttttttt attggttttt gaaagggctt ttctaaataa aaaggaaaca aaaatacgat
     6001 ttacagatat gattaaataa aatcataaga aaaactggag gcgtgtatca ggaatgctgg
     6061 aaaattacat cgagcgaaat atatttagaa aagtgtatct gtgtgaacaa ttatttgaat
     6121 tccaagaaat agacatcgaa caaacagcta tttctctaag ggtgactaca ccaacgatcc
     6181 ttcacgatct agaaagtcta gccgagtgtc tagaatattg tataaaagaa caagtaagag
     6241 agaagcataa gtataaactc gtattcaaac atggtattgc tctttctgag ctcacacagt
     6301 ttctgtacgg tcaatcttat tttttaaagt ttttaagtta ttattttaat ggtatattca
     6361 cgagtacaga actggctgat cttgaattta tttctttgag caaagtatat acaatcaaaa
     6421 aaatcgtatt ggattttttt aaagaaaatg attatttgaa aaataaagaa atcattatcc
     6481 cagaatttga ctcgcggaac atcttgctag ctttagtacg ttatgtcaat tgggaaggct
     6541 atgaaaataa aaatccgtat gtagaagaag cttgtaacga gttgattgat tatgtagaga
     6601 ctcatttttt taaaagaaga tattcggaag atgaaaagtc ctttatcata cgaggaatag
     6661 agatcgcaat cggaagaaag gcatttcccc ttggactgaa agaagaagat aagaaaagcg
     6721 cagaaacgaa gccgttattt caattagtct gtcaaggatt ggaagagcaa aaaggaattc
     6781 tagacttaca agaagaagat gtctattata ttttttcttt gttcaacagt agaaattata
     6841 ccaatgagaa catggagctg ttaagaaaag atttcgaagt tgtttataaa aattttgttc
     6901 tcaatgacct atcactgaat gaattgattt tacagattat tagtatcgct gaaatcgacg
     6961 atgcagaaaa ttttattttc aagcaatcct ttttacaatt tgtccgtacg ttgtgggcag
     7021 atggacaagt atttttaccg gaaaggatct atcttctatc agaaaaagaa caacttctgt
     7081 acggaaaaat tgtagaaata ttggaagagt ggaaaaagaa aaatcaaatc caactaagat
     7141 ggaatgaaaa tttcattcga aaattcgtca aaagtctttc tttgatcaac agtgaacaag
     7201 cggggagaca ggaaatagaa atttttgttg tttcagaaag ttcagtcaaa caattttttt
     7261 atcgaaccca atttcatttg tatgcggaag aaggcatcgc tgaaataaat cctattttat
     7321 ttcgttcatt agaagaattg cctgatgaat gtctatgtgc gaaaaaaaga gtggttttgt
     7381 gcgatgctgc tagctatcaa aatggattag aaactgaaaa gacaaagatc tatccaatct
     7441 cattaaaagg tgcaaacatc catttgtatc atattgctca ggaacttcac ctattaaaaa
     7501 aacatatttc ttgatgagac cttaattagg agaccttcta caaagctttt taattgtctg
     7561 tagaaggttt ttatttgagt cctgattcgt gcgaagatct acttatataa caagctttct
     7621 ctgaaatttt tttaaaatgc tattgactta gagtcaactc taagtagtag aatagataaa
     7681 gtgtaacaaa tctattttca ttaaaagaag atagtacgga ggagagaatg tattatgagt
     7741 acagtattac atgtattgga tgcaatcaat agccccgcag atgtcaaaaa attatctgtt
     7801 gaggaaatga aacagttggc agaagagatg cgccaaaaaa tcttgcaaaa ggacagcgat
     7861 gtaggtgggc acgttggtcc gaatctaggt gtagtagaat taacgattgc gtttcattat
     7921 gtatttgatt caccaaagga taaaatcgtt tgggatgttt cccatcagtc ttatggtcat
     7981 aagcttttaa ctggtcgaaa aaatggtttt gaaaaaggac attaccgtga agtgagcgga
     8041 tattccaacc aacatgaaag taaacatgac tattttacag tggggcacac atctacatct
     8101 atcagcttag caacaggttt agctaaagcg agagatctga agaaagaatc aggaaatgtg
     8161 gtagcattta tcggtgacgg ctccctaagt ggtggcttag cttttgaagg attgaacaat
     8221 ggtggtgttt tacaatccaa tctaatcgta ttagtaaacg ataatgagat gtctattgat
     8281 gagaatcatg gcagtttata taaaaactta gctgagcttc gtcaaaccaa cggtaaaagc
     8341 agcaataacc tatttaaagc aatgggattt gattatcgtt acgtggataa tggaaacgat
     8401 ctggaaacgg tgatccgttt atttgaagaa gtaaaagata ttgatcatcc gattgtactt
     8461 cacgtccata cggaaaaagg tcgcggttat cagccggcaa tcgataataa aatgaaatat
     8521 cattggcatg taccatttga tctgtccacc ggggaggcaa agcaagtaac ttctggagaa
     8581 acatttaata tgatcatcat ggattatttg gataagaaaa tgcaaacaag tacagctcct
     8641 attgccgcag tcaatgcagc gataccaggc gcctttggac tgaaaacatt ccaagaaaaa
     8701 catccagatc attattttga cgtggggatc gctgaacagc actctatcac tttcgcttct
     8761 gctttggctt ctcagggagt acgtccagtc gtcttccaat caagtacttt cttgcaacgg
     8821 gcatatgatc aattatcaca tgatttagcg atcaacgaga atccagtgat tattttagtc
     8881 ggtggcggaa cgatctcagc tagtgaccga acacaccaag gaacatttga cattccttac
     8941 ttatcaagta tcccgaacat caaatatctt gctccagcaa caaaagagga tatgatctct
     9001 atgctagatt gggcgttcga acaaacagcg cagcctgtag caatccgaat tcctacacat
     9061 ggtgtagaac atggtacagc tatcgcaaat gattatagta ttccagccta tcaaacagta
     9121 aataatggag gaaaagtagc aattttagct ttaggcggtt ttcttgaatt aggaaaagaa
     9181 acagtaaaag cttttagaaa aaaaggaatc gaagcaacgt tgatcaatcc aggaacgatc
     9241 acagaaaatg acgtagaaac attagataaa ttaaaagaaa cacacgaatt agtcatcaca
     9301 ttagaagatg gtagtttgtc aggaggattt ggcgaaaata tcagtcgtta ttatggtgat
     9361 tcttcaatga acgtattaaa ttttggtgca gagaaagaat tcacggatag cgttcctgta
     9421 gggcagcttt atgaacgtta tcatttaaca ccaaaacaaa tcgttgaaga tagtctgcgt
     9481 gtcttgaata ttcaataatt cattatgctc accatccaga taattgttta tactagtatc
     9541 tggatggttt tttcattata aaaagcaaag gggtggatgt tatgtatcag caagtagaag
     9601 aatatgtcaa aaagttccaa gaaaaatatc ctagttatca atcagaaaaa ttaggtttct
     9661 tttcatttgg ggatacagaa aaaatggcag acgagttgag cgaactagtt gtaaaaaaga
     9721 taaagactgg tacatcaagt ggttacgatc tgtataaaca agagaatgag cctttaccaa
     9781 aagttggtca attagacgtg gtcctgaatg gaaaaaatga accagtttgt atcatcaaaa
     9841 atataaatgt gaccatcttg ccttttagtg aagtcacgaa agagcatgct tggaaagaag
     9901 gagaagggga tcggacatta gcttattggc ggaaagtcca tgctgagttt tttaaacatg
     9961 cttataaaga agcgttgaac atagaattta cagaagagaa gctggtcgta tacgaagaat
    10021 ttgaagtaat tttcttttag taagaaaggt ctgctgaaag aaatagctca ttattgtctc
    10081 ctgaatagaa aagggttaca tttttatatg aagctgtatt tttatataac attactggag
    10141 gagatattgt ggatacactg gaacaaatca aaggcggagt cgtcgtttct tgtcaagctt
    10201 tggaaaacga gccgctacac agttctttta ttatggggag aatggctttg gctgcaaaag
    10261 aaggcggagc agttggtatt cgagcaaata cgacagaaga tatcaatgaa atcaagcgaa
    10321 tcgttgattt accggtaata gggattatca aaagagatta cgatgggtca gaagtgttta
    10381 ttactgcgac agaaaaagaa gtggaagaac ttttggagac gcaagcagaa atcattgcac
    10441 tggatgcaac ttctcgtaaa cgtccgacag aaaaaacaac tgctgaactt gtccaaatga
    10501 ttcacgaaca cggtcggtta gctatggcag atatttcgac ttttgaggaa gcgcttcaag
    10561 cgcaagaaga tggttttgat cttttgtcta cgactttagc aggctataca gaatattcac
    10621 gaaagacgca gacacctgat tttgatttat tgaatcaaat catagaacaa gtcgatatcc
    10681 cagtaattat ggaaggacat actgactctc ctgaacaagt agagaaagca tatgaattag
    10741 gtgctttttc agtcgtagtt ggatctatta tcacccgtcc gcaattgatc actaaaagat
    10801 atgtcgaagc cgcaagtaaa gcaaataact aaaaaacacg cacggcacag attcttgaca
    10861 aaaaagaaaa attgatagat agtttaaagc agattctaag aagatgaaaa aagcattact
    10921 tttcattttg tcttcaagat ctgcttttta acgaatttta tagaaagaag gcgaaaccat
    10981 gtttgaacgg agtccttcag aattgacaga acgtgaaaat tacaagttgc tgattggttc
    11041 agtcatccct agaccagtag cagtagtaac gactaaatca gcaaaagata tagtgaatat
    11101 cgcacccttc agttacttca atatcgtcag ttcgaatcga cccatcttat ccttggcgat
    11161 ccagagaaaa caagatgcag taaaagacac agcaaagaat attcttgaga caagagaagc
    11221 tgttatccat atccttgatg aggacaacgt agaagaagta aataaaacag cagccaatct
    11281 gcctaatgag gaaagcgaac tgtacctgac tgacttgact ttaactgatt cagtaatcat
    11341 ttcagtacca gcattgacag aagcaaaagt tcgttttgaa acatccttat atgaacatgt
    11401 cgagatacgg aataaaaata aagtaacagc tgatttattt ttgctagaag tacagaaata
    11461 tcatttaaat gaaacagtct atgatgataa gacaggaagg atcgatccaa gaaaattgaa
    11521 tgctgtaagc cgtttggcag gaaatgatta tgcagcaata ggagaaattt tcacgattgc
    11581 acgtcccgaa taaaaaggag cactagttat gttaaaaata agctataaac catcgacaga
    11641 ttcaaaagaa atgaaaaaag aatatgaaac agtgaatgat tttctgcaag ggcagtactt
    11701 agaagttccg ccattgcaag accattttgt cgttactact gttactttag atggtaagga
    11761 aatagaaatg ccagatcaaa cgattagcgg attgtttaat tactttaaca aataaatatt
    11821 tattccgaaa agtgaaaaaa catataaaaa aacacataaa actaaaaaat tatcaaagaa
    11881 tcctggtgca actgaaagaa tttgcaacgg gattctttca acttatttgt tatttttttc
    11941 taaaaaatat taaagattgt taaatttcac tatttcattt caaattccat tgcctaatca
    12001 ttaggaaaag tctatcatga actcgtaaat agaatatagt aataaccttg ttaggtgagg
    12061 ctcctataca aacataggct gttgcccaga aacgtcgaaa gtcgccaatg ggtaaaacag
    12121 aaactgttgg attaaggctt tttccaatag ctaagaatgt tttctttacg ttgtatagtg
    12181 ctaaaactcg gacgatgaga tgatgctagg tcagaattct tgttcgaaaa ggtttctgac
    12241 cttttcttta tatttttgtg actgacttat ttgatgagac aaactcgctc ctcacctgac
    12301 aagtggattg ctgtgtgagg aggagatgcg gtgaaaaaag atcgattggt ggaggtcgct
    12361 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
    12421 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
    12481 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
    12541 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
    12601 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
    12661 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
    12721 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
    12781 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
    12841 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
    12901 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
    12961 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
    13021 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
    13081 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
    13141 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
    13201 tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag
    13261 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
    13321 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
    13381 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
    13441 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
    13501 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
    13561 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
    13621 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
    13681 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
    13741 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
    13801 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
    13861 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga
    13921 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
    13981 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaata gcttttgaca
    14041 gtctaggagg aaaagctgca tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
    14101 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
    14161 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
    14221 atgctcctgg atctacgttt aaaatgatta cagcagcaat cggtctcgac aacggcacta
    14281 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
    14341 gatcttttaa aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
    14401 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
    14461 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
    14521 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
    14581 gatatggaca gggcgaactt ctaattactc ctatccagca agcagcaatg tattctgttt
    14641 ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag acaaaagata
    14701 agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg
    14761 tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga
    14821 aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct
    14881 tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag
    14941 aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt
    15001 atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc
    15061 ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactcac
    15121 acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag tgtacttaat
    15181 aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact ttgctgtcca
    15241 atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc gaccatcaat
    15301 gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag caatttggtc
    15361 aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa gtcagaaaaa
    15421 tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata tcctaaagcc
    15481 tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat cccagcaaaa
    15541 aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat tcctaaacaa
    15601 ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt
    15661 aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat tagctgttca
    15721 atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa agataggctg
    15781 aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa aattgttgat
    15841 ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa
    15901 gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg
    15961 cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca
    16021 ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat
    16081 aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat
    16141 ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat
    16201 ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata
    16261 aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat aacatacatt
    16321 attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaaaga tttttttgga
    16381 ttgttggttt ttatacggtc tattttaata actaaaaatg attaacgtat agtcattttt
    16441 cttattctga tgaaattgat aatctcctgg atgcccatga aaatcaaaat aaagccgaat
    16501 atttggaata gaagcatcaa acttttgaat ggattgaaca ataagacgaa tccgccaatc
    16561 aacaacagaa tactactata aacactccaa cttttagctt ttgtattcat gctgaagaac
    16621 aactggaaca agctgttgat gataacagct aatccaagaa gaatcggcag gatagaaacg
    16681 attgcaggtg caaaagcata gacgatcaga gctaagatca ggaaaaaaag accactgaac
    16741 agaccgatac tccaagaacc tgttttcctt ttaaaacggt aagcctcaaa aagattcaac
    16801 aacccaagca aaacaagata ggcggtgatc agataaccga taaaatgaaa aacagcagaa
    16861 gggtttagca caatcgcaat cccagaaata atataaacag cagctcttaa taaggcgtat
    16921 ttttgaatat tttgtttaat cgtctcaaac atataagaaa acctcctttg acttatttta
    16981 acgtgaacta gtcattttac caaattaaat gttacaaaat gagcgaagga gaagaatatc
    17041 tctggaaaaa tcaacttctg aggatctttt ccattttttt gccttttgct agctcatcga
    17101 tcattttatc taaataacga atttctttca tcaagttatc ctcgatgtct tctactcgta
    17161 caccacaaac aacacctgta atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa
    17221 aaaattgttc gaaagtcact tgtttttgta gttgatattc tagttctgtt tgggaataac
    17281 ccgtcagcca acaaatgatt tcatctactt cctcttttat acgatctttt ctctccgctt
    17341 ttgctacata cataggataa acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca
    17401 taatcagtca cctttctttt tttcaaatta tttactactt atagtatata agaaaaagag
    17461 aagaaaaagc gattattcaa aaaaacaaaa aggattgtga caaaattctt gtcaccatcc
    17521 ttttttccgc tctctgactt tttacaatct tcatagctca agtgcgcatc agataatcga
    17581 atgctcctaa agctgcagta gctcctgaac ccattgaaac aatgatttgt ttgtatggac
    17641 tatctgtaca atcaccagct gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg
    17701 tcactatttc atttcttgga ttcatttcga tcgttctatc tagccaatcc gtattaggga
    17761 gtaagccgac tagaatgaat actcctgaaa cagctagtgt atgcatctcc atcgtacggc
    17821 gatcgatata ggagagagat tctacctgat tcgtaccgct gatttctttc gtcgctgcat
    17881 gtttgataac agtgacattc gcaagagaga ctagtttatc ctgtaagatt tgatctgctt
    17941 ttaattctgg taagaattct agaacatata catgttttgc taacccagcc aaatcgattg
    18001 ctgcttcaac accagaatta ccaccaccga taacagcaat ctcttttcct ttaaataaag
    18061 ggccgtcaca gtgtgggcaa taagcaattc ctttattttt aaactctttt tcgcctggta
    18121 cattgataga acgccagtgc gcacctgtcg cgatgatggc ggtttttgct ttaagtgata
    18181 taccgttagc taagccaata ttaataaaat gttgctcttt atttaaggaa acggcctgtt
    18241 gattcatcat aatgtcgata gggtaactac gcacatgact ttctacttgc tgcatcaatt
    18301 ttggaccttc tgtgtaagag gtaccaatca tattttcaat ccctagtgtt tcaacgactt
    18361 gtccgccgaa agaatcagcg acaagtcctg tacggattcc tttacgtgct gcatagatag
    18421 ctgcactact agctgctggt ccaccgccaa tcactaatac atcataaggt tctttttcaa
    18481 acaactcttc ttcaatcaaa gggccagtga ttttttcaac aatttgttcg atcgtcatgc
    18541 gtccgctact aaattcttct ccatttaaaa acacagttgg cactgccatg atttttttat
    18601 catcgatttc tttttggaac atcccacctt caatcatcgt atgagtaata tttggattta
    18661 atacagctaa tatattcaat gcctgtacaa cgtcggggca attatgacaa gtcaagctaa
    18721 cataactgtc aaaatgcaat gtttgtgtta tttttttgat tttaagactc aaagattcac
    18781 tgattttcgg tgcccgtcca ctaatttgta acatagccag taaaaaagag gaaaattcat
    18841 gaccgagggg gataccagca aaaacgattc cactttcttt gccaactgga ttgatagaaa
    18901 aactaggtgt gcgcatcgaa tctctttttt cccaagagat tttaggagac atttctgcta
    18961 tttccttcgt aaattcagct agtttttgac tattttcact attatccaaa ctaacagaaa
    19021 aaacaatagg agattcaatc agtgctaaat attgttcgag attttgttta gtttctttgt
    19081 ctaacatatc caccacctaa atctttccaa caagatcaaa acttggtttc aatgtttcgc
    19141 cactttcttt ccattttgcc ggacaaactt cacctgggtg ggtacgtaca tattgagcag
    19201 cgcgaatctt atcaatcagt gcactagcgt cacgaccgat tccatctgca ttgatttcca
    19261 tagcttgaac gattccgtct ggatcaataa taaatgttcc tcgctgagct aatccttgct
    19321 cttcatccaa aacatcaaaa atacgagaaa tctgatgaga cggatcaccg atcatcacgt
    19381 attcgatttt accgatagca tctgatgtat catgccatgc tttgtgagta aagtgggtat
    19441 cggtagagca agaaaagact tcaacaccta aagatttcaa tgtactgtat tgttcttgta
    19501 agtcttctaa ttcagtagga caaacaaaag taaaatcagc tggatagaag caaagaatac
    19561 tccagttccc cataagatct ttttcactga cttctaaaaa ttctccttga tgataagcat
    19621 gtgcattaaa ttcttcgatt tttttcccaa tcaatgacat agtaaaacct ccaataataa
    19681 ttatttattt ataattattg taatttagtt ggagttgcaa agtcaatgga tattttgtta
    19741 tttaataaag attactcaaa aacgaaggtt aattataaaa atagaagagg tcgcaagaaa
    19801 atgctactgc tggaaatact cgggataacg ttgagtaaca gtttcctgtt ctttcagcat
    19861 catgtgtaag tgcaatcccg tttgaaatcg agcttcttct ggttttttat caactaaagc
    19921 cttgagtaaa tcataatgtt gttccatgat cgtttcccat ggaagcccct gaatggtcac
    19981 tcgtaaccag cggaaacgtt ctaaatgggt attatggtca tctaaccagt tccaaacttc
    20041 ttttcttcct gcaatttcga aacaagcttc atgaaataaa ttatctgcac ggaaaaaagc
    20101 gcgttcgtca tttattttga ctgctttttc ttgttcttct aaaatcgttt ctagcactat
    20161 tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg atttgctctt ccaactgttc
    20221 tcggacaaag cgagcatttt ctgcagaaac aagattaatt ttggaaacat acgtaccact
    20281 ttgaggtatc gtatacacga gttcttgctg acgtagttga atcaatgctt cgcgaatggg
    20341 agtccggccg atagcaagat gttcctccag tgccttttca gatattttcc ttccaggttc
    20401 taagtcagca tagataattt gtttacgaat cgcttgataa gcttgtgatt gcaagctgct
    20461 tttcatcttt attcctccaa aatcatttct atgaacgtag tataacagaa gaggtctgaa
    20521 aaacaatctg gtatgtcaca ttaactagta aatttgtata aatagtaaaa ttttggttga
    20581 catagcgatt tttattgcat aaaattcact tgaatacaaa aactaatatg ttagtttagg
    20641 aggggaaaaa tgattgattt aaacaactgg aggaacttta tccctgaatt atctcaaaaa
    20701 gaagtagaaa tacctcattt tgaccgtgca tcattaagag aaaatggaca aaaaaatcct
    20761 gtctggatcc atttcggtgg cggaaatctt tatcgaggat ttcatgcaga aatcgctcaa
    20821 cgtttagcgg atctaggcga attgtccact ggtgtcattg tttgtgaaac attcgatgaa
    20881 gaaataattg aaagggctta ccaagatttt gatgatttga ttcttgaagt ggtcatgcca
    20941 agcagtggag agttcgtcaa acgtatatta gctgcgacag ctgacagtct gtattgtcat
    21001 ccagattgtt ctgaacactt tgataagatg attcgacact ttgaaaatcc tagtttacaa
    21061 ttagttactt ttgctattac tgaaaaaggt tatcgattga aagatacaaa taataaatat
    21121 ataggaccga ttgcagtcga tataaaaaat ggtccaaaga agccatcaca tacaatgagt
    21181 attcttgctg tgctacttta taaacgtttt ttatctggag cttggccgat tgcacttgtc
    21241 tctacagata atttttcaca aaacggtagt caattacgta cttctttgct aacgatcgca
    21301 aaagaatggc aaaaggaagg ttatgtacaa aatacattta tccaatactt atcgaatgaa
    21361 gaaattgttg cgtttccatg gacgatgatt gatcgtatca ccccgaatcc ttccttgtca
    21421 gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt cgattttcca aaccgcaaaa
    21481 ggaaataata ttgctccatt tgtcaataca gaggacatcc attatttaat catggaagat
    21541 cattttccca atggacgtcc gaaattcgaa aaagctgggg tattcttgac tgatcgtgca
    21601 acggtagata aagcagatac gatgaaggtg acgacttgcc ttaatccttt gcatacgtct
    21661 ttagctatta tgggttgttt actggggtat aggagcatag ctgaggagat gaaagatcca
    21721 gatttatcgc tgctagtttc tgaaatcggg tacaatgaag gcctcccagt tgtggagtct
    21781 ccaggtatca ttgatccaaa gatctttctg gaagaagtaa ttactgtgcg cttttctaat
    21841 ccaaatattc ctaatatgcc tcagcgaatc gccacggata cctctcaaaa ggtggcgatt
    21901 cgttatggtg agacgattaa aaaatatatg gaaatagaag ttaaagaccc gcagacactt
    21961 gtattcatac cattgactct cgctgcttgg cttcgatacc ttattggcat tgatgatcaa
    22021 ggaaaaccat tcatcccgag tccagaccca ctattgacag accttcagca taagctttcc
    22081 gatattcaac tgggtacgca agatacatca catattcatg agaaaatggc tcctattttg
    22141 tcaaataaag aaatttttgg tgtagatctt taccaagcag agcttggcag aaaaatagaa
    22201 gaaatgttca tcaagatggt agcaggacca aatgccgtac gaaaaacatt acaggattac
    22261 ttacaagaac atggaggaaa tttatcatgg aaatgacttt tagatggtac ggatcacagg
    22321 aagaaaagat taccttagat gaaatcagac agattcctgc ggtcaaaggg attgtaggca
    22381 cattgatgga tataccagta ggagaggtat ggcctaggga gaggatcaaa gaactaaaag
    22441 aagaaattga gtctcgtggg ttaacactaa aagtaattga aagcgtcaat gtgcatgatg
    22501 ccattaaaat tggattacct tcccgtgagc agtatatcga aaactataag cagacaatcc
    22561 gaaacttggc agaatatgga atagaagtca tctgctataa ttttatgcct gtatttgact
    22621 gggtgaaatc tgaccttgac taccgcttag aagacggctc atccacgtta gcttttatat
    22681 ctgatgatat tccagatgat ccaagagaaa tcgtcgcgcg catcgaacat tccagcaatg
    22741 aatttggatt gccgggatgg gaacctgagc gcttagctcc tatcaaatcc ttgcttgaag
    22801 cctatgcatc agtagacgaa gaaaaacttc gagaaaattt tgcctatttt ctccaatcaa
    22861 tcatcccaac atgtgaagag gtaggggtca aaatggctgt acatccagat gatccgccat
    22921 actcgatttt tggattagct cgaatcgtaa agaatcgaga agatttagat tggttgtgtc
    22981 gcatagtaga cagcccttat aacggaatca ctctttgtac agggagtatt gcagaagatc
    23041 cagaaaatga tgtatatggg attttagcag aattttgcca aagagatcga atcccatttg
    23101 cacatgttcg aaatattaaa tttttagcta atcgagattt ctatgaggca ccacataaat
    23161 ccgaatacgg ttcattagac atgtaccgca tactaaaaac aatgtatgac aacggattta
    23221 acggatacat ccgtcctgat catggaagaa tgatctggaa tgagacagga agaccaggat
    23281 acggcttata tgatcgagca ttaggtgctg tttatctaaa tggattatgg gaagcaatcg
    23341 aaaaaggaaa caaaagtaaa gataattagt gaaaaaaact gtaattttat tatgtaaata
    23401 agaaggatgt tcaaattctt gatacataaa tccggaggtt gttaataagc attcggattt
    23461 atgtatctta tttggtctag gaagtaactt gattttcttt tttcgattga tccatgagtg
    23521 cttcttcttt taattcaaag atatattctg agatcctcat aatcactttt ttccagtctt
    23581 ctggtgtgat gattcctttt aaaatataga tttttttgat atgagaaggc aattgtttca
    23641 aagggcagtc gctgataacg atgttgcagt tctccataaa atcattttgt attttgatcg
    23701 attcatttga aaaaatttgt tctatcttaa cgatcagatc tttactgata taaaaatcac
    23761 gcgtatagtt cacaccaatc acaattcgcg gctgtttttt gtagcgatca tacaaatgta
    23821 ctaggtcttc aaccatccat tgcacatttt ccttttctaa aataactggt aacaagtgtt
    23881 gattttgttc tctgaaatcc atataaaatt gttcaatggc ggtttggact tctttcaata
    23941 cgctatgttc ttgataaagc atgaaagtag gaaggggcgt atttggttga ttgaagttaa
    24001 gaaatccatt ataaagttta tttcgtgcga atgccaatga gtaaagaatc ttttcttttt
    24061 caggtataaa caaatcaaat acagtacaaa gggactccac catttctctt gaataaagaa
    24121 catgaggaac gtttgctttt tttaaaagtt tgtattgctc caagcttgtt tcttccgatt
    24181 cgattttcgt gatagaaagt cgagctaaaa tattcactaa gatgcgttgt tcgcgacaaa
    24241 ttggcagatt aggtcgcaaa atatctacag agagatcaat aaaaaagtca atatattcat
    24301 actctgtgaa ttctttttgt acgtctttca cagaagttgc aggaaaattc tctttacata
    24361 tttttaatag aaaatataat ttatctatca taccgctatt tagtcggtca agtacttctt
    24421 ttttaaaaaa agtattcatc ataaaagcca ctacttgcgg atcttcatca taaaattctg
    24481 tagcagtact tgaaaagatg ttccaataaa tatctaatgc tgcatattga atttgtacct
    24541 cttttccaga aaaagacttt ctgccattat tagaaaagga gatagacaaa ccgtactatg
    24601 ctaaaaatgc attaatcttt ttcattttcg tatacaaata agaggcacta tagtttgttt
    24661 gctgactgaa actgataatc gaatttgtct gacagctgaa caaagaagtc aagatctgat
    24721 aactgactga ttcgttgcag tatcttccaa atagacgata atagcagtaa gccggatcaa
    24781 ttccttcaaa attcaacttt aaaaaaggtt tttgcttatc aatatgcagt tctgttagat
    24841 tgagacgctc aaagtcttgt tctaattctt tgatgtatag taaaaaattt gattcagaca
    24901 tatggaaatg atctgctaaa tattgtgtag taataaagaa acgatgttca gataggaaaa
    24961 gaaaaatatc taattttgta ttgctttttt tcttcaagat aaacgtttta atcgcttctt
    25021 gagtttgcat gaaagaggct cctttttact taaattgtta aaacttgaaa atattactgt
    25081 ttttttcata aatttagaca aaaaaacagt aaacatatag gtagaatctc tactatctta
    25141 tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa ccatcttaat
    25201 atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc acacagagga
    25261 gatagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa ttacctttca
    25321 ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac aaacagcgac
    25381 catcaaagca aaaattgact attcaggaga cttagcacct atactacaat cagctgttac
    25441 atttgagaat gttgatcccg ctttgtcgat tggctatgac gagaacactt tgactatcca
    25501 acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt taaaaccagc
    25561 gctttttact gtcaaggtat cagatggact accgaacgat catcatcatt taacgccgta
    25621 tagtcagcgg cagacaattg agcaagattc agctatcgac tcaggaggag atcttgttga
    25681 agaaccaaca gataaaccag aaaatgaaaa taagccagaa gtaccgccta cagaaaatcc
    25741 agacggagaa cagaaaccgg aaatagaacc tggagaagaa cccgatacag aaactcaacc
    25801 agagccagac aatgaatcaa aacctgaaat aacccctgga gaaaaaccag atgtagaccc
    25861 agaagaaaaa ccagatgtga ctccagaacc agatacggat tctggaaacc aaacagtacc
    25921 ggaaacaaat ccagacacag ataatgaaac tgaaaatcca gaaaaaccag aggtagcccc
    25981 agaagaaaaa ccagatgtaa ctccagaacc agatacggat tctggaaacc aaacagtacc
    26041 ggaaacaaat ccagacacag ataatgaaac tgaaaatcca gaaaaaccag aggtagatcc
    26101 agaagaaaaa ccagatgtga ctccagaacc agatacggac gcaagagacc aaggaatacc
    26161 agaaaaaata aacaaaaaaa caattcaaga ggatggaaaa aaagagagta aaaaatcaaa
    26221 tctagcaatt ctaaaaataa atgaagagca gctgaataaa aaatcacgaa tatttgatag
    26281 cgcccaatca gctgaaacct taaaaagtag caaggataca acctttgctg tcctgaaacg
    26341 aaaaataaac agctaccaaa atccggagag tcacaaaaca aagttatact gtggagcgga
    26401 atcattctat taagtatagc aacaatgctt tctgcaaaaa gatttaaaca aaacagatct
    26461 ctctaacatt tgaataatgt ctgtgaacgc atttttcaaa taaaaagaaa tatccagaaa
    26521 atgaccccaa aataccagat tgaaatctga tatttggggt cgttcatact ttatctttct
    26581 tcatatttat tttaagggat atctgtttct tttggaagat ggttcgggat ctttttcact
    26641 ttcaatacat ctaaaaagaa ggtaaagatc ggaatgccga caatcaagcc ccaaacgcca
    26701 aagaaccgtt cgctaacaag taagaccaca aatgtataaa aaatcggcag ttctgttttg
    26761 ctggacatca acttcggatt cagcacataa gactcaatta gatggacaat caaaatcgta
    26821 atcaggatat aaacaacatc tcggatgcct ccttgggaat aagctatgaa agatagagga
    26881 atacaagaaa tgataacacc tgctactggg atcagactta aaatgaaaat catgatcgct
    26941 aaactcagca gctgatgaaa gccaaataca gccaaggcga ttgtagtgag caacgtattt
    27001 aaaagagcga caacaaattg agcttctaat accaaaccaa atgtattgac aaacttatcg
    27061 gcaaaatagt aaatatcttg aaagaaccaa gaaaattcac tttttaaaaa caatcttgag
    27121 aatacaattg tttttttctt ttcaatcata aaaaagaaac ttaagatgaa tgacatagca
    27181 aacgccactg ccatttttcc tatatcctgt aaatagccaa gcgcaatcga agcgccattt
    27241 tgaagctgtt caaaaaaatc gtttcgctca atataagtat ggatatattg taaaacagga
    27301 tctgcatctt ttggctgatg ctcgtaaaaa ttaataacgg actggatcat gtcaaatgtt
    27361 tgatgaataa gcatcgggat atactttgtg aatgccagat agataaggta aacgatcagt
    27421 gtataaatca tgatagctaa aacacctgta gggattcgga caaatcgctg aatgatttga
    27481 acaagatgaa tcatcaaata agtaaagata aatgtgagca aaatggttgt aatcatactt
    27541 ctaactaaga aaagaacaaa aacgacaaat aataaaacag tgagacggcg tattttttca
    27601 ttcgccaaaa aacgctgata aacggtcaaa tcgacaacct ccaaataaca aaaaataaac
    27661 acgttattca ttatatgatg gaatatgaaa ataagaaaga gcaaaaagaa aaaccgctaa
    27721 atgacgttat cttatgagtt ggttttcctc ataaaatcgt tcatctagca gtttcagccc
    27781 tttttctggt cttgatgaaa attatctagc atttttcggg tcagatgttg cagtgtatcc
    27841 aattcttcct gttcaagtgg aagataagac ttgatctttt gtggaatacc ttctgcttgt
    27901 ttttgcaacg cagcaccttg agcagtcagc gtaacattta cgattctttc atcacccgtt
    27961 tcacgctgac gattaatata atttttttct gctaactttt ttagtaaggg agtcagcgta
    28021 ccagaatcca gacacaaatc atttcctagc tctttcactg aagtcttttt cttttcccat
    28081 aaaactaaca tcacaagata ctgggtatag gtaagattca gcggcttgag gaggggagtg
    28141 tagtgtctaa tgatttcttt agaggcggca tataaaggaa aacatacttg attaactaaa
    28201 tatagatctt ccatttttcc acctccgctg ttttactata gtgtatcata gcggatttac
    28261 acaaaaaagt ctattcacta actgctttca gagtaacttc tatatttcca gctgtagctt
    28321 ttgaatacgg acacacttta tgcgctgcct ctaataattc ttgcgtttct tccagagaaa
    28381 gaccttcgat agatccttca atctctacac ctaagacaaa gctcgtttca ctttctgaat
    28441 aaagggaaac ttgtgcactg acagtagaag ccccagagat atttttttgc tgtttgatca
    28501 attctaatgc actgttaaaa catgcactga aaccagctgc aaacaattgt tcagggttgg
    28561 ttgcattttc gacgcgttta ccaggagctg caatattcaa ttcgaatgta cgatcgggat
    28621 tatgcacttc tccagagcgt ccgcctgtgt tgatcatttt cgtagaatac atttttttca
    28681 taaaattacc acctttaatt atattttata caattgaatt ttacacaata taattgtgga
    28741 aagcaaagaa atggattggc ataagataaa taagagaaag cacatgaaac agacgcgctt
    28801 tctcgaatag caaacatata caaaaaatat cagagacaaa aaataatctc atcataaaca
    28861 ttacatatat ttctcaaaaa gttgttttct ttcttcaaaa attacttcac gatggacctg
    28921 atgaagcatc cataaataac caaaaggatc tctgaacatt gagttagtca caccaaagtc
    28981 catcatttct gtcatttctt gaatctctac acagccattt cgtaatgctt tattatgtgt
    29041 gtcctcgata tcagagacga tgatattgaa ccagatcgta tttggatgtt cagcagtagg
    29101 agcagtcaaa ccaagagacg tgttttcatc cagcatatgg aatctcgtac catacaaggt
    29161 aaaaatgact tcattattgc ctctagggta atctgttgcc tcaatcagtt gtatttcatc
    29221 aaagattgtt tgatacagtt ctagtgcttt caaactatca tttaatacaa aatcgatttc
    29281 cgttccttta tgcataaaga tctcctctcc ttttcagtcg aacagttact agattctttt
    29341 attataccag ctaaagaaaa cgcattcaaa aaaatagcgg atttacttac caactcattc
    29401 gattggatga taagtaaatc cgcgtcccat tacttctgct acgctgctga caatgacaaa
    29461 ggcacttgga tcgatttctt ctatcaatgg tttcattcgg gaaaattcat gatcagtcac
    29521 aacgatcaat aaaacctctt ttgactttcg attatacccg ccttttgcct ctagcagggt
    29581 aaggccacgt ccaatctcag cacttaagcg aaggcggatg tcttccaaat aatgttcact
    29641 catgaccata acagatttct ttcgattgat acctgtttcg atataagtca tgacaattga
    29701 agtgattgca atagataaaa tagcataaag aaactcttct attccaaaaa taaacagatt
    29761 aagagaaaca acgaatgcgt ctgtaaaaaa taagccaatc gaagtattca agtggaaata
    29821 ttttttaaaa atgaggggag ggatcgtcgt accgccgcta gaagagttgt ttttatataa
    29881 aatcgcaact cctaaagcaa aaatcgcact tccaaaaatg acagacaaca accgatctga
    29941 agtaatcatt gtttctggaa ctattgcaat gattaaagga aaaaccaagc ttccgaaaag
    30001 tactttaaaa aagggttttt tacctaaaaa tagaagagct agtaccaaca taacgatatt
    30061 caacactaaa ataactatag ctcggtcaaa gcctaaagca gcttccagta atattcctag
    30121 accgctgaca cctccagcag caatatgatg aggccctaaa aacatattga tagcgactcc
    30181 taatatgaag agggcaaaaa taatcttaat gtatgtccat ataaattgtc tcgtttcttt
    30241 catttattca cttccttaat tcattaataa tactcttatc ttattataac ttacttttca
    30301 ccttaagtta actttaatta ataaaaaaat taggctagtc taaattatta tgtttcattc
    30361 ttctagaata tacttctacg ccaaaaactg ttaaaaaact tttactttag aaaattggag
    30421 aaattgatcg cttaagataa attcaggaca ctaaatagta caggaagtag actaaaactt
    30481 ttaaaagttt taagtctatc tatttggcaa gtttaaaaaa taaaaattcg gtatcaaaaa
    30541 actgttaccg ataacatttt tgaaagcgtt gtcttttgct tctctatcac ttatagtgat
    30601 gacgtcatat taaaacacaa ggaggaaatc aatggataca gctgcaattt atcatcgacc
    30661 ggaaagtgaa tatgcatttc tatatacgaa tacaagattt cgtattcggc ttcgtactag
    30721 gaaaaatgat atcaaaaaag tatatgtgct atgtggagat ccatatacca taacaactga
    30781 aaaatggtat caaaaacaac gaccaatgaa aaaatgtcta agtaccaatg ttcatgatta
    30841 ttgggaaatt gaagtgacga gcgaaacaag tcgcttacag tatgcgttcc atgttgttgg
    30901 ggaagataac acggactgtt tttacggtga tcaaggaatt tttccttatc gagaaaatgt
    30961 gattactgaa ccaaattttt attttcgaat accttatttt catcaaatcg atcgattcac
    31021 tattccagaa tgggtaaaag aaacagtatg gtatcaaatt tttcccgaaa gatttgcaaa
    31081 cggggacaaa gcaaatgacc ccgaacacac tttaccttgg ggaagtaaag atccagaccg
    31141 agaagacttt ttcgggggag atttgcaagg tgtgatggat catttggatt acttgacgga
    31201 ccttggcatc aatggcatct atttttgtcc catatttaaa gccacatcca atcataaata
    31261 cgatacgatt gattattatg aggtggatcc cgcttttggc gataaaaaac ttttaaaaaa
    31321 cttgattgat gaagcacata aaagagggat acgtatcatg ttcgatgctg tattcaatca
    31381 tatgggtgtc cattcaccac agtggcaaga cgttctcaaa aacggagaaa aatcgatcta
    31441 taaagactgg ttccacatcc gttttttccc agtagattct tatcagatga ctgacttacc
    31501 agaaactgct gaaaatatcc catatgatac ttttgctttt actccattca tgccgaaact
    31561 aaatacagca aatccagaag tgcaaaaata tttgctggat atcgcaactt attggataaa
    31621 agaattcgat attgatggat ggcgtcttga tgttgctaat gaagtcgatc atcatttttg
    31681 gaaaaaattc agagaagcag ttacagaaat caaaccagac atctatatcc ttggagagat
    31741 ttggcattcc tctcaagctt ggctgcaagg ggatgaattc catgcagtga tgaattatgc
    31801 atttacagat tcgattaaag attattttgc aaaaaagaaa atcacagctt cacaaatggt
    31861 cagtggaatg aatcatcagc aaatgcttta cagagatcag gtgaacgaag gaacattcaa
    31921 cttattagat tctcatgata ctgccaggat cttgaccctt tgccaaggta acaaagaatt
    31981 gatgaagtct gtgatggcct ttatgtttct tcaaaaaggg tctccttgta tctattacgg
    32041 gacagaaata gggatgactg gtgaagatga cccggattgc cgcaaatgta tgatatggga
    32101 gaaggaggaa caagatctag agctattcgg ttttataaaa gaacttgttt ctctaaggaa
    32161 acagttgtct aagattattt ctgaaggttc tactcagtgg ctaatagtaa atgatcgtga
    32221 agataagctc tatttcacac ggaaactaga gggacagatc atctatgttt actttaatca
    32281 gtcaaaagaa ccttgggtag tagaacagga aaatgaggtg attcttagtc aaaattgtca
    32341 gttgttagaa gacgggaaag cagagatacg acaatacgga tttttgatca tgtgttgaaa
    32401 actgttaccg gtaacaacaa gaaagcgttt gcaaatgaag atttgtgccc attataataa
    32461 gggtgtaaca gcacgttgaa tacaaaggag gaaatgaaga tgaaaaaaag aagtgtgaaa
    32521 cagttgggtt tagggttaat ctctttagga atcttaggcg gattagcggc ttgtggcaat
    32581 ggtaactctt cagcagaagg ggaagccgat gaaggaaaaa cgttaacagt ttccgttgat
    32641 tctggatata aagattatat aaacgaaata aaaaacactt tcgaaaaaga aaacgatgtg
    32701 aaaatcaagc tagtagaaaa ggacatgttc gatcagttgg aatcattagc gttagatggg
    32761 cctgctggaa agggacctga cgttatgatg gcagcatatg accggatcgg tgcgttagga
    32821 cagcaaggac atctggcgga agtaaaactg ggaaatgaag cagcttacga tgaaacagac
    32881 aaagcccaag tgacttatga tggaaaaatc tacggagaac ctgcagtaat cgagaccctt
    32941 gttttgtact ataacaaaga tttagtcgat acagcaccat caacgtttaa agatttggag
    33001 aatctatcca aagacagtcg ttttgctttt gagtcagaag caggaaaaaa tacaggattt
    33061 ttagctaaat ggactgactt ttattattcc tatggtttaa ttgcaggtta cggtggctat
    33121 gtatttggtg atgatggcac aaatccttca gatatcggtt tgaataatgc aggtgctgta
    33181 gaaggaatct cttatgcaac agattggttc aaaaatgttt ggccaaaggg aatgcaagac
    33241 ataaaaagtg caggagactt cgccagccag caattcatgt ccaataaaac tgcagcaatc
    33301 attgacggtc catggcaagc gcaaacatac aaagaaaaca atatcaatta tggtgtagcc
    33361 aaaattccta ctctaaataa tggacagcct tatcaaccgt ttggcggagg aaaaggatgg
    33421 gtagtcagca attatgctaa aaacaaagac ctctctcaaa aatggcttga ttatgtaacg
    33481 aatcaggaaa atcaagaaaa attctttgaa atggtaaatg aaatccctgc aaaccaacaa
    33541 gctagggaaa cagcaaaggc taaaaacgat gaattaacaa cagctgtgat tgaacaatat
    33601 gaaacagctc aagcgatgcc aaacatccca gaaatgggcg aagtttggac aggtgcagaa
    33661 aacttgatgt tcgatgcagc atcaggttca aaaacaccaa aagaatccgc agatgaagca
    33721 gtaaaaacaa tcagtgaagc catagaacaa aaatactgat taaaatgata accgtccatt
    33781 tttataaaaa atggaacagg tgcttcagat aaaagtggca agagcgagat ggaaaattat
    33841 cttgctcttg ttgccattat cttaaaagca gtttgcggtt tagcaagttt agagggggca
    33901 ttcagatgca gcaagataaa acaagcaata tcaacgaaaa aaaaatcacg ctttgttcac
    33961 ttattccagg gatggggcag tttgtaaatg gccaagcttt taaaggtatc gtttttttcg
    34021 ccatattcgt cctattcatt ggtcagatgc ttctgggcgg atttcatgca ttggtaggat
    34081 tggttacttt gggaagtata ccaatggaag atcattcatt atttattttg attgaaggca
    34141 ctttgcaatt catcattaca atcattttct tgatttttta cggtttgaat cttttagacg
    34201 cttatcgagt agcgaagatg cgtcaagagg gcaaaaaaat caatcggacg atgaaggaag
    34261 tcatacataa tataggtgat gaaggttttc cttatctatt gacacttccc gcttatcttc
    34321 tcatgatttt tacgatcatt ttcccagtcc tagttacatt gttcactgca tttactaatt
    34381 atgacttcaa tcatattcca ccagccagtt taattgattg ggtaggggct aaaaactttt
    34441 ttagtatctt ctttttaagc tcatatcgta atactttttt tgcagtcttt tcgtggaccg
    34501 tgatttggac tttatgtgca acaactttgc aaatcaccct tggtattctc acagcggttg
    34561 ttgctcacca gaaatttatt aaattcaaac gtattttcgg tatcattttc ttattaccat
    34621 gggcggtccc agcttttatt actatcatga gtttttcgaa tatattcaac gatagcgtag
    34681 gtgccatcaa tactcaagtg attcctttca taaaccagct tccttttgta gatatcagtg
    34741 caatctcttg gaaaacggac ccgaactgga cgaaagtagc aattatctta gtgcagggct
    34801 ggttaggatt tccctatatc tatgtgatgg ttacaggaat cttacaatcg atttcagaag
    34861 acttatatga agcagcaaaa atcgacggag caaatgcatg gcagcgcttt tcaaacatca
    34921 cgcttcctgc tgtcttccta gtggcagcgc caacatttat tacacagtat acggggaatt
    34981 tcaataattt ctccatgatc tatctgttta acaatggcgg tcccggaagc gtaggcggag
    35041 gagctggttc tactgatatt cttatttcct ggatctataa acttacgact ggaacttctc
    35101 cgcagtattc tatggcttca gcaatcacgt tgattatctc cgtcatcgtt atctgtgtgt
    35161 cattgctggt atttaaaaag acaaatgcgt tcaatatggg ggattaacaa tgaaaaaatt
    35221 tcattcgagt gctcgattcc aaaaacgact ttctcacttt tttacttact tttttctttt
    35281 aatactgtca gtcattatca tctatccgct tttgatcaca gcaagtactg ccttcaagtc
    35341 aggaaatatt gcagctttta gtttagatct ttcaagtgac tggactttag gaaactttac
    35401 tcggctattc agtgaaacac tttacgggac ttggtataaa aatacattga tcatttctgt
    35461 cctaacaatg atcgtacaag tgactgtagt aactttggcg ggctatactt atagtcgcta
    35521 tcgttttatc ggccgcaaat caagtttgat gttcttttta gtgattcaga tggtaccgac
    35581 tatggcagca ttgactgcct tttatgtcat ggctttctta ttagaagcgc tcgataagta
    35641 ctggttcttg acaatgatct atataggcgg tggaattcca atgaatactt ggctgatgaa
    35701 aggatacttt gacactgtac caatagattt agatgaatct gctaaacttg atggggcagg
    35761 gcatttcagg atttttttcc agattgtatt accgcttgtc aaaccgatgc tcgccgttca
    35821 agcattatgg gcttttatgg gaccttttgg ggatttcatg ttagctaagt tcctgcttcg
    35881 aacacctgaa aatcagactg ttgctgtcgg cttacaaaca tttatttctg atgtgacaaa
    35941 ccaaagagtc tctctttttg ctgctggggc aattttgatt gcactaccga tttgtctatt
    36001 attcttcttt ttacaaaaga actttgttac cggcctgaca tctggcggaa caaaaggata
    36061 ggagcagagt atcatgcaaa catttccgtt aaactatttt tccgcattac gtacgccaac
    36121 tcaagtgttt gcgggacgca aactattatc atggcctaaa tttttcttga tatttgtatt
    36181 tttagtttcc ttgatggtca tgcctgtgac tcttttttat gccaatcaga tccaagcgat
    36241 tccattagaa caatttctat ccgttcattc gctgatcgat gaacaaggaa cccaaaaatt
    36301 ttctgaactt gaattatctg aaactgggtt acaagcatcg caacaaacga tagctgtgac
    36361 ccctgaaatt ttggtaggtg tgtctttatc tgagaaacaa caatccgatc atggaacttt
    36421 tattgatttt gagaaggaac aatgggtgat ccaacaaaag gataaatctg gtatacgccg
    36481 ctatacgatg aattatagcc catctttcca accagattct gtacgtacgc ccgaagattt
    36541 ccaacgattt ttagaaagag aattttatgc cagtaatcgt ccaacaatca ttttatctta
    36601 tagtttaagt cttggtcttg tcctatttgt catgacgagt ctcattttat tcggggcttc
    36661 ctttttcttg tggatgacaa gaaaaagcca actttcatcc attcatacat tcaaagagag
    36721 tgcaaatttg atgctgaatg ttatgggaat aggttccatg gcagcagcag ttgtagggtt
    36781 tatccacttt gactttatct tgatgctaag tgtccaaaca acgataactg tcttgctgct
    36841 tttatgggtt tttgtcaaaa caagattcaa agataagcgt gtcgaatgac aagtttaagc
    36901 gatttatgcg acaatgaatg tgaaaaaaat gaaaaataaa gtagtttttg aaaaatttct
    36961 gaaaaaatta aacttcttag ttgattcttt tcttttttag tattaacgta atagggtagt
    37021 ttttttaagg aggaaaatca tgttagagaa aactttatac aatcaattat tttcacgttc
    37081 tttctcgctc cctgtcgaag taaattattg ggacggtaca acgaaacaat acggagatac
    37141 ggacactcct acccaaatca aaatcaatat ccatgaagaa atcccaatga aagagattac
    37201 aagtaacgca tctttggcac taggagaagc ctatatggat catcgaatcg agatcgaggg
    37261 aagtatccaa gcattgatca acgatgctta tcagcaagca aacagctttt tacgaggaaa
    37321 agattatcta aaatggctgc caaagaaaga aaaacatacg aaacaaaaaa acaaagaaga
    37381 tatccatgcg cattatgact taggtaacga cttttataaa ctttggctag atccaacttt
    37441 gacttattct tgtgcttatt tcaatacacc tgaagatacg ttggaacaag cacaaatcaa
    37501 taaagtccat catatcttag acaaactctt tatcaaagaa ggcgaaacgt tgcttgatat
    37561 cggttgtggc tggggaacat tgatgttcac tgctaccaaa gagtataatg tcaaggcaac
    37621 tgggatcaca ctaagtgaag aacaatacga tttgattacc aaaagaataa aagaagaaca
    37681 tttagaagat aaatgccgtg tattattaat ggattaccgc gaactaaaag gcgaaacgtt
    37741 tgatcatatc acaagtgtag gtatgttcga acatgtaggt tctgaaaatc ttgaaggtta
    37801 tttcaaagtg gtaaaagatt tattgaaacc aaaaggaaca gctttgatcc atggaatcag
    37861 ccgacaacaa ggcggtgcga caaatgcttg gatcaataaa tatattttcc ctggcggtta
    37921 tgtccctggt gttactgaat tagttgataa tattacaaaa aacgatttgc aactgattga
    37981 tttggaaagc ttacgtcgtg actaccaatt gacattagaa cattggacaa agaattttca
    38041 tcaagtccaa gatcaagtca cgaaagaaat gggtgattcc ttctatcgaa tgtgggatct
    38101 atatttacaa gcttgtgcag cttcatttga atcaagtaat atcgatgtga ttcaatattt
    38161 gcttgttcaa cctggaaaca atgacatccc aatgcatcgt gcataattga ggcaaaagta
    38221 ctgtttaaag actatttaaa aagaagctaa gttttatgat gctccagaag tgaacttttt
    38281 tgtctaactt ctaggattgt ctctaaagct tagtttcttt ttctttatat tttcataata
    38341 aaagtttggt tctttaattt ttaaacatga cagaaaattt tttcttcgtt atacttgtaa
    38401 tagaatacta ttttataaca aaggggatcg aatcaatgaa aaaaactgtt atttatacta
    38461 gtttatttgc agctttgttt ttagctgctt gtcaatcaaa taccaatcaa ggcacatctg
    38521 ggaatacgtc ttccagtagc gaacagcgct cttcacaggt atcggactcc tcaacaaaag
    38581 caagcacatc ttcgtctagc cagacaaaaa gttctagtga agcaagtact cagtcagata
    38641 tcaaagtgtc tgcagctcgt gcgatcgaat tgttccaaga taaataccca gatactgcaa
    38701 ttactagttt ggaattagat agtgattggg gatcttactt ctacaagata gaaggtgtcg
    38761 atgatcaaaa agaatacgaa gcaagaattg atgctcaaaa tgaaaaaatg gagacagaga
    38821 atccagaaac tctagatcga gatgaacaaa atggtcaaaa gaaaaaagaa gatggtctgg
    38881 atgttgataa attgatcacg attgaggaag cagcaaaaat cgccgtcgac aaagtaggaa
    38941 gcggtacggc aacagattgg gatttggata aagatttagg tactacttat tgggaagtca
    39001 aagtaaaaga cggcaatcaa agtacatcag tcaagatcaa tagccaaaca ggcgaagtac
    39061 tgtcaactga acaagatgat taagaatttc ttcggaaaac aagagttgat ttttctttta
    39121 gtcatgatac actagacaaa ataacttgtt tgaaggagcg atttacttga gtttagaaac
    39181 gacctttgcc aatcatatct ttgccaatcc tttgatgaat gcatctggtg tccactgcat
    39241 gactacccag gaattagatg aactggctca ttcagaagca ggagcattta tcactaagag
    39301 ctgtacgatc aatgaacgaa aaggaaatcc cgaaccacgc tattttgatg ttcctttagg
    39361 gagtatcaat tctatgggac ttccaaactt aggtttttct tattatctag agtatgctct
    39421 tgcgtatgaa aaagtgcagg aaaaccaaaa tcaaccgtta tttttttcca ttgccgggat
    39481 gagtgtccag gaaaacttgg aaatgcttga gaaaatagaa aaaagtgggt ttaatgggat
    39541 cactgaatta aatttatctt gtcccaatgt gcctggaaaa ccacaactag cttacgattt
    39601 tgaggccact tatgaaacac tgaaagaagt attcagcatc ttttctaagc cgctaggaat
    39661 caaattgcct ccttattttg attttgctca ttttgatcag atggctgata tcttgaatca
    39721 gtttccattg acctatgtca atgctatcaa cagtgtgggc aatggtttgt atatcgatac
    39781 agaacaagaa gcagtagtca tcaaaccaaa agaagggttc ggcggcattg gcggagagta
    39841 tatcaagccg actgctttgg cgaatgttcg cgctttttat acacgtttga aaccagaaat
    39901 ccaaatcatt ggtacaggtg ggattcggac aggacaagat gcatttgaac atctgctatg
    39961 tggcgcaagc atgttacaga ttggtaccga gcttcataaa gaagggccgg aaattttttc
    40021 tagaatcata aaagaattaa cgcagattat gtcagaaaaa ggatatacgt caattgacga
    40081 atttaaaggc aaattacgca ccatttcata gcaaagagac gaaggtcatg attgatcttc
    40141 gtcttttttt ataaaaaaca ctcgtctgtt cattcctgtg caagcgtact cattgtatgt
    40201 tagacgaact tgtgggaaaa taacagaggc aaaaaggagg ataatatgaa tgatgcaaca
    40261 gaattagcaa aagagcttaa agaaaaagaa tttagttttc aagaactagt tacggatata
    40321 cagaaaaaaa taaagaagca aaatcatgaa ttgaatgcgt ttgtcgcgct tgaaccattc
    40381 gagagtaatt cccacgagct attgattccc cagtcagaaa aagggccatt ggctggtatc
    40441 ccatttcctt taaaaatgct cggtcaagaa aaaaagggat ggctagctac ttcaggatct
    40501 cgtttatttg aaacacaccg agcttctcat acatctaatt atgtgcggca agcagaagcg
    40561 ataggtctag ttccttttgg acaaaccaat gcgccagagt ttggtttcaa aaatattaca
    40621 gacccagtca tctatggccc agcaagaaac ccttggaacc ttgaatgtac accgggcgga
    40681 tcaagtggag gagctgctgc tgcagtttca tcaggaatcg ttcctttagc tggtgctagt
    40741 gatggcggtg gttctattag aatacccgct tcgttttgcg gattgattgg gctgaaacct
    40801 tcgcgaggga cgatgccagt tggtccatat gcttggagag gatggcaagg agctgctatt
    40861 gatttcggct tgacagtttc tatgagagac acggaggctt tattttatgg gatgagaagt
    40921 atccattccg gtgcgccata tcaagtatcg ccagttgagt ggcaaacaca tccaagaaaa
    40981 aaacggttga agatagcttt ttgtgttgat tcaccgatcc actcacagat tagtgaagag
    41041 gcagtacttg ctgtaaaaaa agctgtagat ttccttggaa aagaagggca tgatttgaca
    41101 gagattcctt acccactaga tggtcgtaaa ctgatcacgt cttattatca gatgaatggc
    41161 gcagaaacag cagcaatgat ccgctcgatt gaaacaggca tccagcgagc tgtcagaaaa
    41221 gaagaacttg aacccatttc ttggactttg ttccaatatg gtcagaagct ctctgccgct
    41281 acttacatcg attctctgca tgtatgggat gaagcagcaa ttgtcatgga aaacctacat
    41341 caagaatacg atctattcct ttcaccaacg actgcattta ccgcccctaa aatcagcgaa
    41401 gatttgcaaa gtgatgcgat aagagcacag atggaacaca tcagtgattg ttctgaagta
    41461 gaaggtttag acgttgtttc ttccatgttt gaggagagct tgaagatcac accttataca
    41521 cagttggcta atctgactgg acaaccagca atcagcttgc ctactcattt gacaaaagaa
    41581 aacatgcctc taggaatcca atttatggct gccagaggaa gagaagattt gctatttcaa
    41641 atcggctatc tatttgaagc aaatcatcag ttccatttac caaatccatg gaaatagcag
    41701 aaacaaggag aagaagaatg aagaaaaaaa gattgtttgc tctaataact agtgcctttt
    41761 ttattttaag cgcttgcggt acagctgccg ctaatcagga agataacagc tggagtaatg
    41821 tagaaaaaaa gaaagaatta gtagtagcga cttctggcac actgtttccc gcatcttatt
    41881 ataatgatca aaatgaatta gttggttatg atgtagattt ggcaaaagag gtggctaaac
    41941 ggttaaatgt caaaatctcc tttaaagaat acaacgtgga tggacaaatc acttctgttt
    42001 caaaaagaga atcagatttt gctgcgaacg attttggtat cacaaaagaa agagaaaaaa
    42061 aattctccct ttcagagact ttcaaatatt catttgacag tatgatcgta cgaaaaaaag
    42121 atgattcagg aatccactca cttgaagatc ttaaagggaa aaaagcagct ggcgaaccca
    42181 atactagcta tatgcgttta gcggaaaaac acggagctaa attagtcaca tatgacaatg
    42241 ccacgaatga tcagtattta agcgatgtag caaatggtcg aacagatgtt attctaaatg
    42301 attattatct gcagaaaatg gccgtatctg ctttaccaga tatccctgta aaaatactgg
    42361 agaacgttta tttcaatgaa aacgaaagtg gttttttatt gaaagaagga aatgatgctc
    42421 tgaaaaagaa aatcgatggc gtattaaaag agatgaaaga agatgggacg acaaagaaaa
    42481 tatctgaaaa atattttcaa actgatgttt cagtcaaacc gaaagaaacg atggctgagt
    42541 cggtgagtga gtgatgtact ttcctctaat tgatttttcc ctcatgtgga cttcacttcc
    42601 tttcgtactg caaggcttgg tatacacatt agggattggc ttcgtgtcgt ttcttttagg
    42661 aaatctggta gggttgcttt tgacagtttt aggtcttttg gattggctgc ctctgaatgt
    42721 atttattcgt ttttatttat cgttctttag aggcattccc gcactagtcc tgctgtttct
    42781 gttatatttt gggttacctt atcaattaag tgccttaacg gcttcagtca tctgctttac
    42841 gattaccagc agtgcattta ttggtgaaat ctatcgagga tcactagcag gcgtttcttc
    42901 aggtcagtgg gacgctggtt tagcttctgg tttttctttc tttggggtat tgaaatatat
    42961 cgttttgccg caagcttttc gaatctcagt accggcactc agtaacgtag ctatggatct
    43021 agtgaaaggt acttcgttag cagcaacaat cacagtgcca gaaatatttc aaaaagcaaa
    43081 gatcataggc ggtcggacat tcgattattt ttctctctat attttagttg cactgattta
    43141 ttgggtcatt tgtatcggga tcgaaaaagt gcaacagtat ttagaaaaac gttctatgca
    43201 tcagtcaatc tgaaaaatag aaatggtgct agcgtggctg gcaccatttc tattttttgt
    43261 ttcttgtata ttctgcctag tattttaaaa ccatttcatg taaaatgtaa atcgaaaata
    43321 tgttcggagg tagaaaacat gaagaccccg ctaaccatcg aaatagaatc ctcgttatac
    43381 caatattgca tcgagcaagg cggcttggtt gtagaagaag tcacgatgcc tgatgaccaa
    43441 ggaattgtag atacgcttag ctgcttgaca aaatttgatg gaagccgaga atggcgatgc
    43501 tatgagctga aagtgacaaa agcagatttt cgctctacag ccaaactgtc atttgttgga
    43561 cattataatt attttgtttt accaagtaaa ctatatgaag aagtaaaaac tgaaatccct
    43621 acaggaatag gtgttttagt gtatcgtccg tacacggttg aagaggagat gcctgcctct
    43681 ggtacatttc tgattgcgaa aaaagcagca aagaaagagc ttgctgtacc ggaagaagct
    43741 ttgacaaacc gcttcatggc ttctttattc agggaagtaa gaaaagcaaa gcaaatggag
    43801 cgaggcgtaa aatattttcc aacagatcaa ttgtacaaag aattgaaaag acgttcagaa
    43861 aatcaagatc cattttctaa cgaacgatat tatgaacggt ttttagagga aatagctagt
    43921 caggcaatca atgagttgca agaagaagtt atcgcgcttc agcatgatta tgaatatttg
    43981 aggcaacaac agcagatccg tcggttgcct acagagccat tggagtgaaa aatatgtact
    44041 atccttattt aagaggaaaa caatttgacc tattagcatt aaaagaagca ctgtcgcgag
    44101 gattgctctc caataaaatc caaccagtga tcgaaccagt acgtgattcc gctacgctta
    44161 aaaatgtgat cgaattattt caaaaaaagc accatcgaat cgctgtgatc caaaatcccc
    44221 aagtaggaca attcaaatta ttcgatcagc atgtgcacag ttggacagtc aaagaaaata
    44281 gctctgtcgt ccctgcacag atcttaacgc cagaaaatct cagtgaagta ctggattcac
    44341 ctccagcgtt tcttgttttc gatggacagc actacccaaa agatacagag gtgtggaaac
    44401 agttggcagg agttgattcg aaatttttga taccagatac tagtcgcttt cgcatctggc
    44461 tgcccgaaaa caaaattgtg atccgtgact cttttcaaac gagaaagcat gttgaaagtt
    44521 atgcagataa aacagatgac ttttttagtg atgactatct ttttttccac gcagacggat
    44581 atattggatt ttctgatttc acgatcgaag gtagccgtta tttcgataaa gggttcccta
    44641 gccgagcgct tgctatccat ctaacgtata ttgatgctta cggcaatata cgcgtgaaac
    44701 attttgtctc tgacagtaac gactctgcta aagaccaagc attgaagttt cttgaagcag
    44761 gagataaaat gaaaaaatgg atcatgcgtc accatcatca actcttgatt acttcaggaa
    44821 tgattgaatt attggctctt taccagcaac aaaaattccc tggactggga gtgttgaaaa
    44881 agtggtcact tatgcatcat ttggaactta ttagtcacct gctagatcat ccaaaagatt
    44941 ggcttaaata ttattcaaaa gttcaggaac tatatgaagt gatccaatga ttagataatg
    45001 taaatgaaaa aggaatagaa aagaggaaac tggatgtata ttcggcaaac aaggaaagaa
    45061 gaagtagaag aagtggtaga aatcattaat gccgcagcgg atttcattgc ttcgcaaggc
    45121 tctcctcaat ggcaaaacgg gcagaaacca acaaaagaaa cgattttgaa cgatattgaa
    45181 agaaaagaaa gctatgtatt aatagtggaa gatcaaattg ctggaacagc tgcattagtc
    45241 tcaagcgtgg atcccgttta cacaaaaata gatggtagtt ggaaagaatc aactggtcct
    45301 tatttatcga ttcatcgtgt cgctttagca gaaagtactc gaggaaaaaa actagcaaaa
    45361 ccgttcttag aacatttgtt gacgattgca gccagcaaaa atatccaaga tatccgtatc
    45421 gatacttatc cagctaatga accgatggaa aagaccatct attctgctgg attcacttat
    45481 caagggatga ttgaatttcc ttttgaacac ggcgaaagaa aagcatatca aataatcatc
    45541 aattgactat aaaatcatct gagcaaagat aaagacatta acactactga aaaaaacaat
    45601 catatgatag ccaagaggtt gtaacagaag tggccagctt caaaaaacaa gaaagaattt
    45661 ccgaaaatgc ttctcaaatt tttgtggaat tttagcgtat tttacgaaat agctgctttc
    45721 tgaaactccc ttcatcgaga ggtcgtgaca tagcttttgt cacgacctct cggcttatcc
    45781 tacgagtatt attcgataca tttatatcaa taatcctttt tttattttat ctgcagcttc
    45841 ttggaacgct ccatcgtttg acacgactaa aagtgagggg atagctttca aacgtttcaa
    45901 ctgtgctaaa tcttctacat aaaccgccag tctggctgaa atctgttcag atgaatctcc
    45961 acgttttttc aggcgcgatt gcaatgtttc tttagagatc gtaataaaga taggacggac
    46021 taccgtcccg aaatactcat ataaatgcca aaaaccattt gctgtcaaaa cggcgtaaca
    46081 atttcctact gtcagtttct ttttaatttc tgctttactg gaaccatatt tatgttgagc
    46141 atattggtcc cactctgcaa actcatttcc cttgatcatc tgattgaatg tattgtcaga
    46201 aacaaaataa taatcctctt gatctttttc cccaggtctt ggtgatcgat tcgtgtaagt
    46261 aatgatcttt tgttccggct gaaaaatagc ttctgcgagt gaagtttttc cagaaccaga
    46321 aggaccgata aatacatagc aataattcat tgtcatttct cctttgatga ttgaacaagt
    46381 gaaaaagctt tggaaaatat ttccaaagct tttaaaagta actttgcttt tttgtatatt
    46441 ataccagcaa gccagtcaca atagcactta aaaagctgac taaagtcgct gttagcaaaa
    46501 ttttcaatcc gtgttttgcg acaagatttc ctttttcttc atttaatccc ttcatcgcac
    46561 cagaaatgat cccgatagaa gagaagttgg caaatgaaac gaggaagaca gatataattg
    46621 ctgttgtacg ctcagtaaaa tggaaattac catttgctaa ttctgtcata gcaacaaatt
    46681 catttgtaac cagtttagtc gccatgatac taccggcatc cactgcttct ttccatggga
    46741 ttccgctgat gaaggcaaga ggggcaaaga tgtaacctag gatctcttgg aaagtgatgc
    46801 cgaatatccc gtggaagata gcattgatca tggcaatcaa agcaacaaaa ccgataagca
    46861 tagcagcaac cgtgatcgcc acatggaaac catcaagaat gtattctccc aacatttgga
    46921 aaaatgtctg ttctttgttt tcttcaatga tcagctcatc ttctttttct tctaatacat
    46981 agggatttac aattgaagcc agaatataac cgccaaataa gttcaaaaca agggcagtga
    47041 tcacatattt agaatcgatc agtgccatat aagatccgac gatcgacata gaaactgtag
    47101 acatagccga tacactcatg gtgaacaaac gtttctcaga taggaagggt aattcctttt
    47161 ttatggaaat aaaaacttcc gattgtccta aaatagcaga agctactcca ttataagatt
    47221 ccaatttgcc catcccgttg attttactta atcctagacc aacagctttc ataaataatg
    47281 gaagaatctt gatatagcgt agaataccga taatagcaga aataaataca attggcatca
    47341 atacggaaag aaagaaaggt gcacttcctt tatttgctac accggcaaaa acaaagtcaa
    47401 caccttgccc agcaaaagca agaagatgat caaacatttt tgccaataca gaaacaaccg
    47461 ctgtaccaaa agttgtacgt aataatacga aaccaagaac aagctgtaaa acgaacatca
    47521 caattagtgg tttcaatttg attgcttttt tgttactgct gatcaaaaaa gcaagcccta
    47581 atgtgacaaa taaaccaata atacctaata aataactcaa ataatcactc ctattccaaa
    47641 ttccgtttta ttttacctgt tttgcaaata aaatactaat ctttagtgaa attttcatta
    47701 cttttttaga gtgaaacgca ttcattttcc attagaaaat aaagcgaaca aaataaaagg
    47761 tttaaaaagt gtaacgtctt agtaaagtct ataaaaaaac gaaactatcg attaagctaa
    47821 tagtttcgtt ttcaacattt ataagtaaac tttttctatt ggtaatatag attttcggaa
    47881 gggtagacag gtgcgcaggt aatatcgatt cctagttttt tcaatgtttg ttcatcattt
    47941 ttattgacca tcacagtaga gtgggcttgt gctccctcaa gttcagctag tttatcataa
    48001 gcaacttgag cgattggatt cgttactgca ctgattgata aagcgatcaa gacttcattg
    48061 atgcttagtg catccaaaga ggaatgaagt gtctttgatt tcaatccttg tattgtttca
    48121 agaactaatg gtgacaataa aggaatgtca tctgcgatat gggctaaatg ttttactgca
    48181 tttaagataa ctgccgctga cgcatccatc aatgaacttg tacgcccggt aatgatgact
    48241 tcatctggta attcgattgc aatcacagca gaaggatctt ttgtaggact ttgcgcttga
    48301 atcttatctg catagtcatg ggcagggact accacgtttc ggtcttcagg acgtaaaccg
    48361 acttcttcca taatcagttt gattcgatta actgcttctt cgtcgatcaa tccttttttg
    48421 aagtcgcatt ctgtttggaa ataacgacgg atgatttctt gtttagaggc ttcttggaca
    48481 acggtatcat ccacgatccc aaatcctaca cgattcacac ccatgtcagt aggagattga
    48541 taaacagatt ctttaccagt aattttttca ataatacgtt tgattacagg aaatgtctca
    48601 acatcccgat tatagttcac agctacttta ttgtaggcat caaagtggaa ggaatcgatc
    48661 atattgacat cttttagatc gactgtggct gcttcataag caatgttcag cggatgtttc
    48721 aacggaacat tccatactgg gaaagtttcg aacttggagt aaccagctgc atgtcctttt
    48781 tggctttcat gatacagttg gttcaaggaa gtagcgagct ttccacttcc tgggccagga
    48841 gctgtcacta caacgattgg tttagttgtt tcaatatact cattttttcc aaaaccatct
    48901 tctgagacga ttttttctac gttgactgga tagtcttcga tttctgagtg tttatagacc
    48961 ttgatgtttc gtctttctag tttattgata aagactttcg tggcaggctg tccactatag
    49021 cgagtgataa cgacactgtt gattaataag ccataaccac gtaattcatc gattaaccgc
    49081 aagatatcca tatcataagt aatcccataa tcaccacgga tcttattacg ttcaatatct
    49141 ccagcataga cgcaaatcag tatttcagct tgatctttca atttttgcag caatttgatt
    49201 ttcgcatctt cttcaaatcc tggaagtaca cgtttcgcat gcttgtcgtc aactagcttc
    49261 ccaccaaatt ctaaatataa tttgtcatag tggttcactc gttccaaaat atacgctgac
    49321 tgctcttcaa tatatttttc tgaatcaaaa ccaatttttt tcactaggtt ccctccttaa
    49381 gtactaaagc attttttcat ttttttgagg attcgtcaac tggaagagcg aaattcctca
    49441 aaaaaacaaa gaaaatggtt taaaccaact tgtctagata gttattcatt ggtatgatag
    49501 tttttgataa ggaaggtgct acatgaaaaa aagacagaaa aagaaaaacg catataaaca
    49561 ctatatccgt tcgattttta ccggttatga aagaatgctt gaagatcccg aattggaaca
    49621 attgacgttt acttatctaa acgaagaaac gcagctaact cgtgatgagc atcagcgcat
    49681 tcatttcaca acaagagatc tgcctagtaa ataaaaaata agataaaggt catcggattc
    49741 gattctgtcc tatcagatgg aaagactagt tgatcgaaaa aaaggctgta ccaaattttg
    49801 gtgcagcctg ttttatggag cattgcccgc tcttttttat aacaattttt tacagaaaat
    49861 ggtactattg aagtattaat aagtgaaaaa cagatgagta agaggaggca agtagtatga
    49921 aagagccaga tacaaatgca aattatgagc cagacgaact agaggagacg aatgcggatc
    49981 aggatacagg acaagaatcg tcttcttttt acgatatcaa agaaaaagca agcatgaatc
    50041 tttcccaatt attagaagac ttacaggctt ttgaaaaagc tgtccgcgaa gaagatatcc
    50101 ctgagatcta ccgtttatac aatggccgat tgaatgaaga attaaaaaga agctcaaatg
    50161 aaaatcacga gatcgatcaa ttgctgatgc gtaagatcca tgatcgtttt ttacaggaat
    50221 ttccttttat ggaacaagta gaaaacatct cgccaacgat gagctattac aaaatcggca
    50281 cttattacca tgatcggccc acggtcggta ttgatgcaag tctgccagaa atttttgttt
    50341 tgcctaagat cgatgaagag tgggagaaat attcccaacc ggagtcaacg gactatgaca
    50401 aaaaaatcaa tgagctagac gcaaaggtga tcactgctca gacagaaatt gaacgtttag
    50461 atgaacaaat aaaagaaatc aatcgacaaa tgactgattt gaacgacaat aaaggattct
    50521 tgaatcgtaa aaaaatcgag gaagaaattc aagaattaga gaagaagaag caagtactat
    50581 ctaatgaaaa attaggctgg cttccttata ttgagacgcc tgaaacaatc cagcagcaaa
    50641 aagaagcatt gaagcaagaa gcaagagcag accagcttcg tgcagccatc gtagaaaaag
    50701 aacaaaggca aatcagacgc tatttcggta gcaaagaggg attcggtcaa gcaatccatg
    50761 aattcttgat gaattatctt ggcaatgaga atcctaataa tcaatctaat gaaggaggtt
    50821 ccgagtatga atgaacacga tgaagcagaa tggacaaaaa atgaagaaca aacagaagat
    50881 catgaagaaa cgaacacttc attggaagaa caaacaagta atcctctgga tgacacagaa
    50941 gaaatcccta tgctagagga aacaaaaata cacgaaaaag aaaaagtaac tccgacgaat
    51001 acgaatactg cagaaaacac ccatgcttac gaaataactg aaaatcccgg gtatttagaa
    51061 ggaaaagagc aagatcatca agatatcgtc agtttgattg aaacagcgga agcagaatta
    51121 gcggatttac gtatgagaaa agatttgatc gaatcagatt ttccacaatt attagaatca
    51181 tatgatcgtt ttatattgaa tgaagaacca atctccacga tcggaaagac aagcctgatc
    51241 gaatacttca cgtatgaatt actgaaaccg ttgaatgata ttggtctgga acggtcaaat
    51301 tcagcttatg acacatggaa aacaagacat ttttcgatca attatacgtt gaatggacaa
    51361 aacgaattag tcttttcctt tgtattaccg accatcgatc aacgatacca agtggaatcg
    51421 atgagtctcc taaacgtatc accagagaca atggaaatca atgtccaaga tgaccgggtg
    51481 ctttctttga tccgacactg gtcagtggat cgagtattct ctgcagggca gatcacgata
    51541 ttcaatcaca aattgaatca attattagcc catgcaagaa cacttggttt taccgttaat
    51601 cagacattac tagacaatac gaagccactt cgtttgaatc ttcagagcga atttgaattg
    51661 acgaatgaag tattggatga tatttttatc gtggcaatga aaaatccatc ctacgatatg
    51721 gaaaagatca acgaaactac ttatcaagta cgattagata aaggacagtc tttaacgata
    51781 acaacaaacg aaaaagatca aacagtcttg tcgatctcat ctggagacta cccacgatct
    51841 gtcattgatt tctttatcaa ttacgaattc ttggtcccat taatggtgag aaagtcctga
    51901 ttggataagt tggattacca gatgtcattc agataattct gttccaaagg acttgcggca
    51961 gttatccaga ataattgtca caagtctttt ctgtatgtac taaatggacg tgaataactt
    52021 gctgatgaag gagggatttc attaggaatg tccacaaata aaatagcaca gcttagctta
    52081 ttgtctgctg cttgtgtagc aggtaggatc gcttttcaat ttattccgaa tttccagcca
    52141 atgactgcta tttttttatt tatagtactt tacttaagct taaaagatgc gttaattgtc
    52201 atgagtcttt ctattgcgat cagcagtttt tattttggtg ttggaccatg ggtcatcggc
    52261 caatggatca gctatactgt tgttctgagt ttgttttcaa tcgtctgctt gagaaaaaca
    52321 gtgcaaagga atctatggtt aaagggagtc tgtttttttc tggcgggcat ggtttacgga
    52381 ataggtatga ccgtttttga tacgctgtta tatcgtttgc ctcaaccttg gatctattac
    52441 cttcaagggg tgtcgtttga tttgatgcac agtataggca atgttgtgtt ctttcttgtt
    52501 tttctgccag tcgtcaaacg tttttacaat cattcaggag gaaaaaatga aaaaaataat
    52561 ttatagtttg cttgcaatga gtgtcagtat actcattttg acaagttgca gtacaaccta
    52621 tacacaatcg gcttccaacc aaaaggagga agtaaaaaca tcgcaaaaag caacaatcac
    52681 gttacaagaa aatggtcaaa acttttcgga aaaagaagtg ttcttcaaaa aaggcgatga
    52741 tttactgagt attttgaaac gaaattttga agtcaaagaa gataacggct tcatcacttc
    52801 gcttgaaggg cactcacagg atgagaaaaa tcaactctat tggatgttta ctgtggacgg
    52861 gaaaagtgct actactggtg ccaaagagat caaattgaat gatggacaat cggtcatttt
    52921 caatttgtct aaactataag tttcataaaa aactgttaaa gggcgaatat tgccataatt
    52981 cagtgagttc agttgatgaa agaaggaaaa taacatggaa aattttaaaa aaagcggaat
    53041 gttttccgta attgctgctc taggggctaa tattctagtt gctatcagca aatttgtcgg
    53101 ctatgcaatc agtggcagtg ccgccatgct aaacgaaagc atccacagta tcgttgattg
    53161 cagcaatcag attttgctgt tattcggtga taaacaagcc aataaaggac agagcgagtt
    53221 gcatcaattt ggtgaaggac gagcaaaata ctttttcagt acaatcgtcg caatgatgct
    53281 tttcttcggc ggtggggcac ttggggtgat ggaagctttt gaaaagttga cgcatccatc
    53341 ccatgaagtg ggtaatccga tcattgttat agcaatactg ttatttggtt tggtaatcga
    53401 aggaagttcg ttacgcgtgg caatgaaaga aatcaaagaa ttgaatactg aaaaactttc
    53461 cacgatgcgc tttttgcgcg aaagtcgaca cagtgaaata ctgattattt ttacggaaga
    53521 cttgtgtgct gtaatcggtt tggtcttggc tttagcagga acaatattga cttatgcaac
    53581 cgggattgct gcatttgatg cgatcagcgg attgctgatc ggtttcttgc tgatgggagc
    53641 cgccattttc ctagcaaaag aattttatag tctgatcatc ggtgaaagcg tgacagcaag
    53701 cgatttatca aaaatcaagt tagcttttga acgtcctgag atcagtcggc tgatcgatat
    53761 aaaaacagtt catttaagtc caacggagat tttagttgca gcaaaagcag atatcgttgg
    53821 taacaaagag aatgaagctt actcggtcat caatgatatt gaaaaaaata tgcgtcagtc
    53881 tcttcctgac aagaagatgt atatatacat tgaaacagat aaatatgacc caaattattc
    53941 tcgtaaataa aacgtttatt catgaattga agcaaaaatg agaaaaatat aatcttgttt
    54001 ttaaataaaa cggctacaat ttagcgaatt tatgttacaa tgagtccgct gtttatttgt
    54061 tgtaattaaa ggagatattt taatgaacaa aaagaaaagt gcgccatggc ttcaagcttt
    54121 gcatgcagtg atgccacttt gtataagcta tataccagta ggattagctt gcggagtatt
    54181 gttgcaaaaa gttggatttg atcctctctt atcaggactg ttatcgattc tggtcttttc
    54241 cggtggcgcg cagtttttag ttgcatctat gcttacgacg caagcatcat ttgcaacgac
    54301 attattgatg gttttctttt tagaattacg ctatacatta ttaggagcaa gtcttgccgg
    54361 ttttatgaaa aaagaaaagc ggacgttttt ggctgtcttt tctcaatcac tgaatgatga
    54421 aaactatgcg gtgaactact taaagttttc aactgaccct tcctggaata aaagaaaggc
    54481 tttatatgtc aattggttct caatgactgc atgggccggt agcaatatga tcggtaattt
    54541 tttcggctcg gtgattcgag tagatgctga ccttgtccat tttgctttga cggccatgtt
    54601 cattttcatg tttatcatgc agatgaaaag cgcactactg atctttacag gtttattctc
    54661 aggcgtatta ggggtcgtat tcatggtatt attccaaaat actttcggat tgattgcagc
    54721 aacagtcatt gcttcatttg ccggatttat gctggagaag gcattcaaaa aagaaaaagc
    54781 taagaaacta agagaccgcg gtaaagatgt caatgagccg ctcttcaact tcccggaaca
    54841 ggaggccaat caccatgact aaactatatt tgctactttt agttgcttgt ctgttcatcg
    54901 tggcatatat cccgcgcttg ttcccgatgc tttattttac tcatcgaaaa gtgccatcct
    54961 ggttcagtga ctggatgaaa tatgtaccgg tggcattatt tgcggcgtta gcttttaaag
    55021 atgttttcat tactcatgaa catctggata ttgcttggaa tatcaaaata gctgcaatga
    55081 ttctagtagc aggcgtggct tacaaaacac gttcgatggc gttgtcagtt ttgacaggac
    55141 ttgcgtctgt ctttttgctg tctatgcttt aaactgtttt attttattaa aaacagagat
    55201 tgcgacataa gttgttcaaa ctgatctatc cgaactgcat ttgcaacagt caattctgga
    55261 tgcaagttta ttcgtaggga taaaaaagat tagtgttggc ttatgatgca agctcttttt
    55321 tattttagga attagagagg gatttgtcct tctatgattt tttgctataa tagggaagat
    55381 gtttatttta tgaaagaata gttgagggaa tttaaatgaa taaaaaattg atgcaaggaa
    55441 cattttggct aacctttgct aatcttctat gtaaagtttt aggggtagtc tatttgattc
    55501 cctggctaag tatgatgggg aacaatcaag atggtatgct tgctacgaca ttgtataatg
    55561 ttggctatct tccttatgga ttgttcctga tgcttggtac agtcggattt ccaaatgcga
    55621 ttgcaaaaaa agtagctgta gccacaaaag aaggagataa cgctgcttgt cggctgattt
    55681 ttagaagtac gatcaatatc atgtttgtca tcggtatcgc ctctgctgca ctgatgtatt
    55741 tatttgctcc tttactagcc ggaatcagtc cgattgctaa cgttaataat gggatactag
    55801 ctatcagaag tttatgccca tctttgattg ctatcccaat tcttagtgca atgagaggat
    55861 atttccaagg aaaaaatgat ctgcggcctt atggaacttc tttgattatc gaacaggctg
    55921 tgcgagtgat cgttatcttg gctggtactt attacttgcg cgtattgact gacggaacca
    55981 tattagaagc tgtattgatc agtacagtcg cttctttctt cggtgggtta gctgcgatta
    56041 tccatatgta tatcgtcgga cgcagaaaag acttcttcga acttaaagat tttttgattt
    56101 ccagccgtta tttcgaaaga gaaaaccgat ctgcctctgt ctctatcatt cgggaaacac
    56161 taccgtttat ttttgtcggc tcagtcatta ctatcgttca aatgatcgac caagtaacga
    56221 tgaaaccact gcttcacttt ttccgtccag agatcgcaga tcagcaactg gaattcttat
    56281 tcagccgtgc ttctgtcaat ccaaataaat tgacattgat cctgatctcc atggtaggaa
    56341 cagtagctat aagcagcttg ccaatattaa gcacgatgaa gaaaaaagat cggatacaaa
    56401 tcgaaaaaac agtaggagat agtttttcga ttgcgttatt gattcttctt ccttcattga
    56461 ctggaatgtc tttattagct ggaccactat acacattgtt ttttggctat gatcctgaaa
    56521 gtgtcggata tttccaaatg gctttgctag cttctttgtt tttctcttta ttcacgatcc
    56581 tatctacgat gcttcaatct ttgaatcacc atcgttttgc gatccgatta actgtagaag
    56641 caattatcct gaaagtcatc ttccaagtaa tcggattagg attagttggc ggatacggga
    56701 tgagcttatc aaatggctta gcattcggta ttgtctttgt tcgcggctat cactttatgt
    56761 gcaaagaata ccgaatagca cctttagcaa agatcagcaa ttttttctta aaaactttcc
    56821 gctccaccct gattatgctt gcagtatgtt ttgccgtttt ctttttgctg aatcagcgat
    56881 tgtcaatgga atcaaaaagc tatgcaatga tttattgtgt ggctgtagga agtattggcg
    56941 gcttagtatt tttattttct caattcggaa aaaatgggtt tcgaatgttg aaaaatgtta
    57001 aacatcatag cagaagcaaa gaataggtta actaatcgtt ttttgctgtc acttgaaaaa
    57061 cggcaactcg tatgattacg cttgtcgttt ttctattttc caattttagt ttcatatcta
    57121 atcttactta tgatatagtt ataaggtctg gaaaaattct ttgaaacgag gaaattaaac
    57181 acatgaaact tgcagtagtt acagatagtt cggcatatct gccggaacgt atcaagaacc
    57241 atcccgactt attcgttatt ccaatccctt tgattatgga tgggaaaatt tataacgaag
    57301 gaatcgatat cgaagcggat gaatattatg gacttttaaa aaacagtaaa gattttccta
    57361 ctacttcaca acctgtcgta ggggaagttt tgggtttata tgaggaattg aaagagaaag
    57421 ggtatgacac gattttaagt atccatcttt cttcagggat ttctggcttt gtcaacacat
    57481 tatttgcgat gaaagatgac atcagcggag taacggtcat tccttatgac tctaagatca
    57541 ctagtatgcc gatgggccac atggtagaag ctgccttaga cttaaatgat gaaggtcagt
    57601 cccttgatga aatacttgca catatcgatc gaatccgaga taatacctac gcttatctga
    57661 ttgtagatga tttgaacaat ttagtacgag gaggacgttt gacaaatggt gcagcgctga
    57721 ttggcagctt attaaaaatc aaaccgatcc tgacattttc tgaagggaaa attgttttat
    57781 tcgaaaaaat ccgttcaagt aaaaaagcat ttgctcgagc tgaagaaatt attggtagaa
    57841 gaaataacga aatacaaccg ccagtcaaac tttacgtgat acatgcaaat aatttagaag
    57901 tagcgattgc ggagaaagag aaattgcaga aaaaatatcc aaatgcagaa atcgaaattg
    57961 gtcatttcgg accagtcatt ggtacacatt taggtgaaaa agccatcggc attgccattt
    58021 cagcgcaata attcctttta ttcaaaaaac ataccaaaaa tattccaaca tcatcttgga
    58081 ctttctacat gttactaatg agcgtgtaaa agaacgagat ggtgttttta tttctatcaa
    58141 tgaagctctt tttctttgca atcctcactt caatcctcta taatgtcgca tatagtttag
    58201 taaatgagta aactactaat ttacttattt actaaaaata aaaggatgag gagttaaaat
    58261 aatggaaata ccaacaaatt taaaacataa accagtaatc gttgtagaag actatgataa
    58321 agttgatgga agaaatgcat taaatacgga tgcaaaaggg ctttcactgg gccttgctca
    58381 gtggaatgat cgtggaaaag tcgatatctc aggcaaggta tggcgacata ctggagaaaa
    58441 atggtcacga cagtcggaag aattaccaat cacacgtatt ttagatttag cgatcttagc
    58501 tgcacaagga agtttatact tccaagatgc ttatcgttat gaaaaatttt atgatccaga
    58561 aaatccagtc gtagacatca ttggattaca agggaaccga atgacggtag aagtagatac
    58621 caaaaatcca aagatcgatg aagacatcat tttgtattac gatacactac aaaaagacgg
    58681 agaactgatt ggggaacgat tccgtatttt aaaacgtctt ttagatgagc taggatatta
    58741 atagacagag atgatcaaaa ggggaagaga agcatggaga atttctcact aacagaaaac
    58801 cgaacctttt cacggctaga aaagcaaatg atctggaaga aaccgaaaag ccatcggaca
    58861 agtgcagaag aatggcggat cgcctccgaa atcaaaggga attggcaaga tcctgaaatg
    58921 aaaatcagta acgtactttt ggaaggagat gccgggtcgg ggaagacaca attagccaaa
    58981 gcactatctg ctgatctgca acttccctat acaaaagtga cctgttttgc agatatggac
    59041 aaatcagatg ttttcggagc cttattacct gtagtagatt ctaataagga acaagaccaa
    59101 gaattactag aagctattta ccaaacagat acacttgaag ctgtactgac actgatccag
    59161 aaccattacg gcattgatcc tttttctgct aaagaaaaat tagcagagct agttcaaagg
    59221 atcgaagctg attatccaga tgcaatccat tacagctatt acccttctga aatcgttcga
    59281 gcgattgaaa aaggatattt attagagatt caagagccga ctgtcatccg agatgcctct
    59341 gttcttgtgg cacttaactc agcgctcgaa ccaaatggga tgttgaattt accgacagga
    59401 atcattcgtc gccatccaga ttgcgtcatt gtgatcacta caaatcgaaa ttaccaagga
    59461 aatcgcccat taaatgaatc tttaagagat cggatgcaac atgcggaaaa aatggatctt
    59521 cctgccttag aagtcatggc agaacgtgcg atggcgaaaa ctatgatcca aaaacaagaa
    59581 cttctcttaa agatggcaga gattatccgt ttgttggatg aaactgcaaa agctaacgcc
    59641 atcaaaggtg tagcaggaat gcgttcttat ttttattggg caaataccta taagcaagga
    59701 caagatttac tccagtcgat ttatccaaaa gttttgtata aattatccac tgattcagat
    59761 gaattggcaa ttcttgaaca agctttgtct gacagtggat tgctactgga acttcaggac
    59821 atattgagaa aagaaaaatg gggtcatcct caatctgagc atacaaaagg aagaaccatt
    59881 agcgccgaag aagcaaatga acgaaacatc actgaaaatg ctgaagaaat tctgcgctta
    59941 gatgaaaaga aatcgttgtc tgctgaagaa ccaaatgagg gagaagagaa acaagaagaa
    60001 acaatagaaa aaaacgagat tcctcctcaa atgcaaaaag aacgcagtag cgatatcgaa
    60061 ggaaaaaatc aaaaagaaca acaaacagga gatgattctc aagaaatgtc tgcttctgac
    60121 atggaagcac atgataagga aatcaagaag caactgaata aagaagcgcg acaaatcatg
    60181 aaggaaacga ttcatgaaaa agaaggtctc atcgttcacc gaccagcttt ccaaggaaaa
    60241 acgccggaag cacgtgagct acacatgcaa gtaatgccaa tagtagaatc acttagccgg
    60301 caaattttag agttactgga taatgaacaa acagagacgt accaaaaagg aaaatacgaa
    60361 ggtcagcggt tcaatgcaag caaagtagca tatggtgatt tgagaaattt tgacaaaaag
    60421 aacccgccac atgagcaacc ttctttagcg gtagctgtac ggatcgatga atctgggtca
    60481 atgatacggg atgatcggat cgaagcggca aaaaaagcag cgattgccat tgccgaattt
    60541 tctaagaaag tcgatatccc attgttgatt tacggagata cagcagaccg ttcgtcaaga
    60601 gaaaagactt ctttgttttc ttataaagaa tttgaagacg gatttcaatg gttaaatgaa
    60661 aaattagtca ctatgaaacc aaggcaaaac aatcgtgatg gtgctgcact tcgtttagca
    60721 gcagaaaaat taaacagaca atccgcaaca accaaactgt tgcttaatat tagtgacggt
    60781 cagccaaaag cgttgccgga ttatacaggc aaaaaagcaa aagaagatat ccagtctgtt
    60841 ttgaaagaat atgaacgtca aggaatccta tttgtctccg cagctactgg ccaagacaaa
    60901 gaagaaatca agtcgatcta cggtgaaagt cgttttgtag atattactga cttgaatgaa
    60961 tttcccaagc aaatgattca actgattgcc cgctatctat gataaaataa agctcaacga
    61021 acgcacttag cagattcgtt gagcttttcg tttctagtta ccggtaacaa ggatacttct
    61081 aaaatagaat cggttataat caagagggac cgttaaagga gtgaaacgat atgatacaag
    61141 aacaaaaatg gtggaaagaa gcagtaggct atcaaatcta tcctgctagt ttcaaagata
    61201 gcaatggcga tggtcaagga gacatcaatg ggatccgtga aaaacttaat tatttgaaag
    61261 atcttggtat cggctttatc tggatcaatc cgatctatca atctccattt gtcgataatg
    61321 gttatgacgt aagtgactat caagatatag aagagagatt cggaacgatg gaagattttg
    61381 atttattgct aaaagaagca catgaattag ggatcaaagt gattatggat cttgtcatca
    61441 accatagttc ggatcagcat caatggtttg aagaatcgaa aaaatctaaa gaaagcccgt
    61501 atcgcgatta ttacatttgg gtcgacgggg tagatggcaa agaaccgaat aattggacat
    61561 cgattttcgg cggttctgct tgggagtatt ctcatgaaac tggacagtat tatctgcatg
    61621 tttttgcaaa agaacaacca gacttaaatt gggaaagcga gaaattgaaa gaagaactat
    61681 tcaatatgat ccgatggtgg ttggataaag ggatagatgg atttcgttta gatgcgattt
    61741 ctcatgtaaa aaaagatgaa tattcagtaa aagccacaga gaaccctttc tcaccctttc
    61801 aaaatgtctc aggaatcgaa gaacatttga ccgatttgaa acatgttttt gaagagtacg
    61861 atatcatgac tgtcggagaa gcaagtggtg tcactgcaga agaaggacca caatgggtag
    61921 ggaaagacgg ttatttcgat atgatattcg aatttgatca tatccatatc tggcagcaag
    61981 aaaaagaagg gcaattagat gtccttaaat tgaaacttgc attaagcgct tggcaaactt
    62041 cattggatgg tatcggatgg aacgctttat atatggaaaa ccatgatgtt cctcgtgccg
    62101 tttcagtgtt tggagatact cgtcccgatt tttgggctat gtctgcaaaa gcaatcgcta
    62161 tgatgtattt ctttttgcaa ggtacgccat ttatttatca aggacaggaa atcggaatga
    62221 cgaatatgcc ctttgaatcg atcgatcaag ttgatgcagt agattcgaaa agactttata
    62281 aaaggctgct tgcggaaggt aagacaagag aagaagcatt ggatatcatt cgtgaaacaa
    62341 cgagagacaa cagcaggaca cccatgcaat ggacttcgga acaatatgct ggtttttcta
    62401 cacatgaacc ttggctgatc actaatccaa ataccaaaac gatcaatgta gaacaacaag
    62461 aatatgaacc agagtcggtc ctgcaattct ataagaacat gatccgcatt agacaaacca
    62521 ataaaggtct gatatacggt tcttataaag aatatcttca tgaacatcca caactttatg
    62581 tctatgaaag atatttagaa gatgaacaat atcttattat ggtaaactta acagaatcac
    62641 ttgctgacta tgaacttcca aaagaagcag atcagtcctg gacattactc ttgtcaaatt
    62701 cctctagcgg agaatttgaa gctaaaggca tactagcacc ttatgaagct cggttatata
    62761 aaacaaacaa gtaaaacttc ctcgttaaag ttcattagtt ggactttgcg aggaagtttt
    62821 tcagattgct ctgtcaatta ctcatttcaa taattcgctc taggaccacc gatatattta
    62881 ggacgagagc gaagggctgt cacgtgagct tgtccaaggg ttttcaaacg aggtctgaca
    62941 ggtacttgga catgtttgat atgcataccg attgatgtgt caccgatatc gataccggct
    63001 tgtccaacta cgtgttccac ttcgacagga tcattgaact ggtcaaaagc agcaacagaa
    63061 caagcacctc cagcatgtag tgcaggaaca acagagacaa tttcgaaatt ttttgcttct
    63121 gccactgttc gttctactac taatgctcgg ttcaaatgtt cacaaccttg gacagctagc
    63181 gcaatttctt taggatccaa caactctttt aacgtacgga tgatccattg accaacttcc
    63241 gcactggaat tttttccaat atgtcctcca acgacttcgc ttgtgctgca tcctaaaaca
    63301 aaaagatcac cttttttcaa gtgtgcctca gccaatatgt cattaactat ttcagtcaat
    63361 tggtctttta attctttttc tgacaacttc attttctaaa accttctttt tcaattaata
    63421 ttgcttccgt gcttgaatac gttgaagact tgcagttata ggaaaggcaa gtcccattcc
    63481 aatcagattt tggaagatgt ttcctggtat agaagccact ccagccggcc aatcatataa
    63541 aatccaaccg gcgataccat agccgatgat cataactaag cttcctaata aaagccctaa
    63601 tccccacata cgagtagaag atttgctgaa agaaccagca acatatcctt gtaacccgtg
    63661 gatcactaat gaaaaaactg cccactgcgg atatcctgaa atcaaatcga tcaatagacc
    63721 actggaagca ccaacaacta atccacccgt tggaccataa agaaaagcag cggtatagat
    63781 cccggcctcg cataatgtca caaatccatt cgtagccggt acagggatca aaattgttaa
    63841 tgaaagggca actgtcaacg caatcagcat agcatagatc gttaattgct tagttttcat
    63901 ttctcattcc tcctttttga ataccgccag accagacact tccggtatga tcgttcatca
    63961 gcacgccatt agaaatacaa tgggcaacaa attttttact ttctgctact gctttggtaa
    64021 aatctgcccc taacacaaga ttcgctgtta ttgcagaaga aaaggcacaa ccggccccgt
    64081 ttactgttgt agtgtcgatt tttttatgag aaaatacctc ataatgatca attgaataga
    64141 gaagatccac agcttcatct ccagccatcc ttgtgccgcc tttgatcaca atgttttttg
    64201 ttcctgaagc agtgagttct ttagcgagat ggatcatttc tgttaacgaa gagggaagag
    64261 cttgaccaga aagtaaagca gcttctgcca aattaggcgt aatcatcgta acaagtggaa
    64321 aaagttcttc gatgagctgc gaaatataca attgattgat cacagcttca gtttctttga
    64381 atgccaaaac agggtctaaa atgattggga tattttgttt tgtttttatg aagtctcgaa
    64441 caaccatgat tgcttcactt gaattcaata acccaatttt gataccatct aatgaagtca
    64501 ttttatcaat cgaaactaac tgtcgtttaa gcaattccaa agatatagat tccagcataa
    64561 atcgctcatt aatatctagt gcccctatag aagtcaaggc gcttagtccg aatagaccaa
    64621 aattctcaaa agtttttaaa tctgtttgga ttcctccacc agcaaatgga tcagaaccgc
    64681 caattgtcaa gattcttttc ataacatttt cctccatgaa taagagtata acgaaatttt
    64741 caaaaaagaa aaacagccaa ttggcatatt tttatctcat ccaatttaaa aaaacaaaaa
    64801 gaaaagaaag accaaaatgg atctttcttt ccttaaatag ttaggacttt tgtgatttta
    64861 attctttctc gatttgaagc cgttgcactt tcattgtagc tgttcgcggg attgcatcaa
    64921 aatcacggat gatagggacg ttcaagcgag gaagttcatg gacttgctcc caccatcgat
    64981 tccaatccat ttctttatca ggagctaaag caataatcgg ttgtggagaa tgatttttat
    65041 cccttacaat cactacttct gccaaaaaat ctaacgaatc taataaaaag tcttcaatag
    65101 ctaatgtact attgatcgtt tcgatcaaat ccacttgccg atctttcaaa aataggtggc
    65161 catgcttatc cataaaaccg taatctccgc tatcccacca atcgccataa acattttcct
    65221 ggaaccgtgc atcttccttg taataagtca aagcccgtcc tttagacagt aattgtatat
    65281 gaccatctgt gttttcaggt aggacattcc caactgaatc tgtaatccga gcgcttgtca
    65341 tgtcttccag tccaacgccc atatctcttg catcacttgt tttcaaactc tctagtgtat
    65401 gagccttaag aatcattggt ccgcattcac tttggccata tacttgaaga aataccgcat
    65461 cctgtgccat cgacgttcgc aaaaatgtct tcattgtctg gttgtttata gcatcaaaag
    65521 tggagtgata gtatcggata cccgaaaaag cttctggatg ctccttagca gtaaaacgcc
    65581 actgaacaaa gttattcgga tgggtctcta gtgcaattgg ccggtgagct tccaacatat
    65641 gaacaacttt tgaactttgt gcgtttgcta aaggcatcat aggaaatccc atggacatca
    65701 aagaagagat accaatattg aatctggaat gcactggaga gatgtgaaaa gcgattaatt
    65761 tcttttccga aatttttgtg aaaactgttt tttgccattt cgtccgccat cccatagaat
    65821 gagcagaatg acaaataagt ttagggatac cagttgtacc agaagtatgg gtcatataag
    65881 aaatttcatt tttgtctaaa aatgattgag aaacagacaa agcaggttgt tccattaaat
    65941 aaagaatagg aa
//
LOCUS       DASHRQ010000007        65820 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.007, whole genome shotgun sequence.
ACCESSION   DASHRQ010000007 DASHRQ010000000
VERSION     DASHRQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.007"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(60..404)
                     /locus_tag="U2M88_000488"
     CDS             complement(60..404)
                     /locus_tag="U2M88_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEM8372895.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(541..1686)
                     /locus_tag="U2M88_000489"
     CDS             complement(541..1686)
                     /locus_tag="U2M88_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEM8372896.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(1866..2840)
                     /locus_tag="U2M88_000490"
     CDS             complement(1866..2840)
                     /locus_tag="U2M88_000490"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEM8372897.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(2920..3795)
                     /locus_tag="U2M88_000491"
     CDS             complement(2920..3795)
                     /locus_tag="U2M88_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEM8372898.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            3925..5832
                     /locus_tag="U2M88_000492"
     CDS             3925..5832
                     /locus_tag="U2M88_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEM8372899.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(6373..9651)
                     /locus_tag="U2M88_000493"
     CDS             complement(6373..9651)
                     /locus_tag="U2M88_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEM8372900.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(9922..10467)
                     /locus_tag="U2M88_000494"
     CDS             complement(9922..10467)
                     /locus_tag="U2M88_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEM8372901.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(10479..11171)
                     /locus_tag="U2M88_000495"
     CDS             complement(10479..11171)
                     /locus_tag="U2M88_000495"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEM8372902.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(11190..11474)
                     /locus_tag="U2M88_000496"
     CDS             complement(11190..11474)
                     /locus_tag="U2M88_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372903.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(11487..12050)
                     /locus_tag="U2M88_000497"
     CDS             complement(11487..12050)
                     /locus_tag="U2M88_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEM8372904.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQKPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            12278..12940
                     /locus_tag="U2M88_000498"
     CDS             12278..12940
                     /locus_tag="U2M88_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEM8372905.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            13064..14260
                     /locus_tag="U2M88_000499"
     CDS             13064..14260
                     /locus_tag="U2M88_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEM8372906.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(14361..15737)
                     /locus_tag="U2M88_000500"
     CDS             complement(14361..15737)
                     /locus_tag="U2M88_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8372907.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(15774..16640)
                     /locus_tag="U2M88_000501"
     CDS             complement(15774..16640)
                     /locus_tag="U2M88_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HEM8372908.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(16729..17754)
                     /locus_tag="U2M88_000502"
     CDS             complement(16729..17754)
                     /locus_tag="U2M88_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEM8372909.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(17772..18446)
                     /locus_tag="U2M88_000503"
     CDS             complement(17772..18446)
                     /locus_tag="U2M88_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8372910.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(18630..19523)
                     /locus_tag="U2M88_000504"
     CDS             complement(18630..19523)
                     /locus_tag="U2M88_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEM8372911.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(19597..21294)
                     /locus_tag="U2M88_000505"
     CDS             complement(19597..21294)
                     /locus_tag="U2M88_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEM8372912.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(21287..22600)
                     /locus_tag="U2M88_000506"
     CDS             complement(21287..22600)
                     /locus_tag="U2M88_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEM8372913.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(22600..26637)
                     /locus_tag="U2M88_000507"
     CDS             complement(22600..26637)
                     /locus_tag="U2M88_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEM8372914.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(26650..27108)
                     /locus_tag="U2M88_000508"
     CDS             complement(26650..27108)
                     /locus_tag="U2M88_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEM8372915.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(27406..27924)
                     /locus_tag="U2M88_000509"
     CDS             complement(27406..27924)
                     /locus_tag="U2M88_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEM8372916.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(27926..28543)
                     /locus_tag="U2M88_000510"
     CDS             complement(27926..28543)
                     /locus_tag="U2M88_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEM8372917.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(28540..29403)
                     /locus_tag="U2M88_000511"
     CDS             complement(28540..29403)
                     /locus_tag="U2M88_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM8372918.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            complement(29390..30301)
                     /locus_tag="U2M88_000512"
     CDS             complement(29390..30301)
                     /locus_tag="U2M88_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8372919.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(30342..31676)
                     /locus_tag="U2M88_000513"
     CDS             complement(30342..31676)
                     /locus_tag="U2M88_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM8372920.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            31884..32714
                     /locus_tag="U2M88_000514"
     CDS             31884..32714
                     /locus_tag="U2M88_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEM8372921.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(32958..33276)
                     /locus_tag="U2M88_000515"
                     /pseudo
     CDS             complement(32958..33276)
                     /locus_tag="U2M88_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(33398..33592)
                     /locus_tag="U2M88_000516"
     CDS             complement(33398..33592)
                     /locus_tag="U2M88_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372922.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(34097..34282)
                     /locus_tag="U2M88_000517"
     CDS             complement(34097..34282)
                     /locus_tag="U2M88_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372923.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(35132..35473)
                     /locus_tag="U2M88_000518"
     CDS             complement(35132..35473)
                     /locus_tag="U2M88_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEM8372924.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(35473..35703)
                     /locus_tag="U2M88_000519"
     CDS             complement(35473..35703)
                     /locus_tag="U2M88_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEM8372925.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            35990..36328
                     /locus_tag="U2M88_000520"
     CDS             35990..36328
                     /locus_tag="U2M88_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372926.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(36533..36772)
                     /locus_tag="U2M88_000521"
     CDS             complement(36533..36772)
                     /locus_tag="U2M88_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEM8372927.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(36748..37095)
                     /locus_tag="U2M88_000522"
     CDS             complement(36748..37095)
                     /locus_tag="U2M88_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEM8372928.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(37827..38408)
                     /locus_tag="U2M88_000523"
     CDS             complement(37827..38408)
                     /locus_tag="U2M88_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM8372929.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(38591..39217)
                     /locus_tag="U2M88_000524"
     CDS             complement(38591..39217)
                     /locus_tag="U2M88_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEM8372930.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(39239..40570)
                     /locus_tag="U2M88_000525"
     CDS             complement(39239..40570)
                     /locus_tag="U2M88_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEM8372931.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(40725..41126)
                     /locus_tag="U2M88_000526"
     CDS             complement(40725..41126)
                     /locus_tag="U2M88_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEM8372932.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(41212..41418)
                     /locus_tag="U2M88_000527"
     CDS             complement(41212..41418)
                     /locus_tag="U2M88_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372933.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(41513..43015)
                     /locus_tag="U2M88_000528"
     CDS             complement(41513..43015)
                     /locus_tag="U2M88_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEM8372934.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(43033..43488)
                     /gene="argR"
                     /locus_tag="U2M88_000529"
     CDS             complement(43033..43488)
                     /gene="argR"
                     /locus_tag="U2M88_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEM8372935.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(43586..44203)
                     /locus_tag="U2M88_000530"
     CDS             complement(43586..44203)
                     /locus_tag="U2M88_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEM8372936.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            44424..45797
                     /locus_tag="U2M88_000531"
     CDS             44424..45797
                     /locus_tag="U2M88_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM8372937.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            45872..46984
                     /locus_tag="U2M88_000532"
     CDS             45872..46984
                     /locus_tag="U2M88_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEM8372938.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(47056..48693)
                     /locus_tag="U2M88_000533"
     CDS             complement(47056..48693)
                     /locus_tag="U2M88_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEM8372939.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(48861..49469)
                     /locus_tag="U2M88_000534"
     CDS             complement(48861..49469)
                     /locus_tag="U2M88_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEM8372940.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(49853..51004)
                     /gene="queG"
                     /locus_tag="U2M88_000535"
     CDS             complement(49853..51004)
                     /gene="queG"
                     /locus_tag="U2M88_000535"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEM8372941.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(51023..51775)
                     /locus_tag="U2M88_000536"
     CDS             complement(51023..51775)
                     /locus_tag="U2M88_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372942.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(51916..52866)
                     /locus_tag="U2M88_000537"
     CDS             complement(51916..52866)
                     /locus_tag="U2M88_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEM8372943.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(52867..53706)
                     /locus_tag="U2M88_000538"
     CDS             complement(52867..53706)
                     /locus_tag="U2M88_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEM8372944.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(53703..54449)
                     /locus_tag="U2M88_000539"
     CDS             complement(53703..54449)
                     /locus_tag="U2M88_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEM8372945.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            54687..56057
                     /locus_tag="U2M88_000540"
     CDS             54687..56057
                     /locus_tag="U2M88_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8372946.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(56112..57008)
                     /locus_tag="U2M88_000541"
     CDS             complement(56112..57008)
                     /locus_tag="U2M88_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEM8372947.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(57122..57292)
                     /locus_tag="U2M88_000542"
     CDS             complement(57122..57292)
                     /locus_tag="U2M88_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372948.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(57289..57966)
                     /locus_tag="U2M88_000543"
     CDS             complement(57289..57966)
                     /locus_tag="U2M88_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEM8372949.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(58057..58962)
                     /locus_tag="U2M88_000544"
     CDS             complement(58057..58962)
                     /locus_tag="U2M88_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEM8372950.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            59137..60591
                     /locus_tag="U2M88_000545"
     CDS             59137..60591
                     /locus_tag="U2M88_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM8372951.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            60607..61638
                     /locus_tag="U2M88_000546"
     CDS             60607..61638
                     /locus_tag="U2M88_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEM8372952.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            61645..62832
                     /locus_tag="U2M88_000547"
     CDS             61645..62832
                     /locus_tag="U2M88_000547"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEM8372953.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            62988..64370
                     /locus_tag="U2M88_000548"
     CDS             62988..64370
                     /locus_tag="U2M88_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEM8372954.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            64497..64727
                     /locus_tag="U2M88_000549"
     CDS             64497..64727
                     /locus_tag="U2M88_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372955.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(64927..65595)
                     /locus_tag="U2M88_000550"
     CDS             complement(64927..65595)
                     /locus_tag="U2M88_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEM8372956.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT        18866 a        13759 c        11730 g        21465 t
ORIGIN      
        1 aaagttagtc atctaacaat aggccgtttc aaaactagtt gtctttttat atccacagat
       61 cattcgactt tttcgaataa cccacggcta atcatcccat ccatcagaaa ataaaattta
      121 tcttggctca ttttctgttt tggatctttg aaaatattga ctgctttcaa tccatttgct
      181 gtagtgatcg acattttcaa tgtttgaata tcttttgata cagtgatttt taatttgaat
      241 ccttctttgc tttgcggcgt ggcatctaat ggtcggatca attgataatt gcctccgttt
      301 gtttctgtaa atccattatc gcgcaatgta tatctttttg catcaggatg caaacgatag
      361 ctaattggtg cgcccagtac cgttgctgtt gtttcaaatg ccatgatttt cacctcgata
      421 aaattagttt ctctttatta taaaagttta tcctgatttt tactaggttt ttcagttaag
      481 ttccctaaaa aatgaaggcg ttctctcaga catctgttca tataagaatg acttcactag
      541 ttattttttg acagcacgaa ctaattgatc aaggaaatac tggatgtcgt cttcggtatt
      601 tcccaaacca aagctgatac ggatggattc ttttagtatc ggtgtatctt ctccatacat
      661 tgcttctaat acgtgtgaag gctcgatatt tcctgctgta caagctgaac cagtagaaat
      721 agcgacccca ttaagatcaa gcttcatcag tagcaaatca tttgcttcgc caattaagcg
      781 aagattcaac acatgaggga gtttattcgt taaatcacca tttacttgat aagggatttc
      841 gtttgcttct aatgtatcaa gaatcaattg ctggaatcct ttatatttgt tgttacgtgc
      901 ttcacgttct tcaggtgaca gcagttcaac tgcttttgcc ataccccaga tccctgctaa
      961 attttctgta cctgcacgcc gcttttcttc ctgctccccg cctaaaagca atgcagggat
     1021 atttactccg tctcgtttat ataagaaacc gacgcctttt ggcccattga ttttatgagc
     1081 agagatactt aataaatcga ttcctaattc ttctggatgg atcgtttggt ttccgtacgc
     1141 ttgtaccgca tctgtatgga aataagctgg atgatccgct aaaagttcac cgatttcttt
     1201 gattggcatc aaatttccga tttcattgtt cccgtacatc acagagacca agatcgtatc
     1261 ttctcgtaac gcttttttga cctcttcgat cgacagattc cctttttcat ctacaggcag
     1321 atatgtaacg tcaaacccct ttgtctctaa atatttcatg gtattaagaa cagcaggatg
     1381 ttcgatagct gtagtgatta gatgtctgcc ttctttttga cgcgagaacg ctgtttctaa
     1441 gatagctgta ttatcccctt ctgttccccc actgttaaag atgatctcgt gaggttttgc
     1501 ttgcagactg tctgcaatga tttgtcttgc tgtttccaat ttttcatgcg cgtggcgacc
     1561 aaatccatgt atgcttgaag gatttccaaa cgtgtcttgc atgattgccg tcatcgcttc
     1621 aattacttgt ggatgcatgg gggtagttgc cgcatgatcc agataaatag ttttcacttc
     1681 attcatccct tccaaattat tgctaggcac attctagcat aaaatagcga acaaaccaaa
     1741 gaagagctca tttttgtcta gaggacgttt catgggcata gaaaaaagga gctgtgacaa
     1801 aagtcttgtc gcagctcctt gaagctgctc acttctgtca caacttcttt ccctcttttt
     1861 tttgtttatt cgaattcttt ttcttccaga cggaataacg gactcatcgg tgtattttca
     1921 tggatgcgtt tgaccgcttc acccattaat tctgaacatg taacgattgt taagttttct
     1981 ggatgacgat tttcttctgt gaaaacagaa tcagtgatac aaatatcttt gattggtgca
     2041 ttgtctaaaa tacttttcgc atgatcggac aataatccat gtgacgcaca ggcatatact
     2101 tcttttgcgc cgttttcttt taatacattt gctgctcgtg caagcgttgc tcctgtgttc
     2161 aagatatcat ctaccatgat acatgtcttt ccttgaacat caccgatcac ataaccaccc
     2221 tcagttgaag catcatctgc atgatctacg atggctaatg tgctgttcaa atattctgaa
     2281 agactacgtg cacgctgaac accgctgttt tttggcgaaa caactacgat gtcatctccc
     2341 gtcagaccta attgacggta ataatgagca aacaacggca tcgtaaataa attatctaca
     2401 ggaatatcaa agaatccttg tacttgaacc gtgtgcagat ctaaagtcag tactcgagtt
     2461 gcccctgctt cttcgatcaa gttagcgatc aatttcgcag tgatcggttc atgaggtttc
     2521 gccgtgcggt cttgcctagc atacccatag taaggcaata cgacattgat cgttttcgca
     2581 cttgctcgct tcattgcatc gatcatgatc agtagttcca tatagtaatc attgacaggt
     2641 gcattcgttg attgaacgat atacacatga tcccctcgaa tactttcttc gatattgatg
     2701 gaaatctcgc catcactaaa ttgcttcacg acgcttttac ctaattctgt tccgaacact
     2761 ttcgcgattt tttcagctaa tggacggttg ccattcaaac taaaaatctt caacgtgtca
     2821 tcctgatact tattactcat gtagtcctcc ccaaaacttc tctattcttc gatagtttaa
     2881 catatttttc caagattttc tgttaatttc agcaaattct taaggaagtg aaactgttcc
     2941 gttactccaa ctggcaggaa tgactttatg ataatcgccc cagttgtcgt gtactttgta
     3001 ataacgttct cctgtttcag tttcaaaata agcgtacgct gtcacccaat gattgccata
     3061 tgtactgcca agtactttca aaataccaat catcacaggt ttgccttcac gtatacgttt
     3121 tgtagctcgc tgccatgaac ctagaagagt tgctcgtgca cgctcatgat tgccattctt
     3181 ctttaaaaaa ctagaaatcc ctgtactaac ctgaaaagga atcgtaggaa gtcctagtgg
     3241 ttggatctgg ctacgcaatg ccttcgtcaa aaccaacgaa tcagccgtat ttttcttgcg
     3301 aatctcattt ggaagcatcc accccttacg atagtcttga taataggcaa ggagaactgc
     3361 tgccgcgtaa gtaccacaaa gatagcctga gggggtagcc catttcttat acctcaaaaa
     3421 acgttcttct ggcaatccca cccagcaatt tgcgagttcc tttcctgacc ttttttcttc
     3481 gatcaaaacg ggaatatctt cttttctgtt gatccatcta gataactcga ttggctcttc
     3541 attgatcagc ttccattttt tacaagaaag accctcttga taagtatatt ttgctggaga
     3601 atattctacc ggatgtcctg attgatcgat aatcacataa ctttgatctt caaaaaccag
     3661 agctaaaaaa agcaatttac tttttcttga ctgcagctgc tgagtgctgg ctaatttttt
     3721 aattttttta tctactttga aatataaagg tttatccact acttgattca gttgggatcg
     3781 cctccttttt ttcattataa gtgaaggatt gaaaaaaaca aatcagtttg attgttcgaa
     3841 ttttcaattt tttatgttct atttgttaat tccttcattt tgtaagataa tgggcacacg
     3901 aaacaaaaaa ggagcgatcg atgaatgaac aaagaacgat tgaaacaaga tttctatgaa
     3961 gcagtcaatg aagaatggca aaaaacagca aaaatccctg cagataaacc agctacaggc
     4021 ggtttccaag atttagttga cgggatcgac caacttttga tgggagaaac agacgatctg
     4081 cttgcccatc cagaaaaagt gtcttcagaa aaaatgaaac atttccttgc atattatcaa
     4141 ctggcaaacg attatgaccg aagaaatgag ttgcatgctc aaccgcttca acctataatc
     4201 gaaaaagttg aatcattgca gtctttcgac gaattgaata agcaatttcc agaatggctg
     4261 ttggatacat taccattgcc atttaatctg gatgtagatg cagatatgaa gaatgcgcaa
     4321 acgaacgcct tctttgctta tccgccttct ttattccttc cagataagac gtattatgca
     4381 tcagaccatc ccaacggtgc acaactaata ggcgttttct ttgatatggc tgttcagtta
     4441 ttagagcttg ccggcaaaga aaaaagtcag gcagaagcaa tcacggaaca agcagtgcaa
     4501 tttgataaat tgattgctcc tcatgtaaaa agtgcagaag aaaacgcaga ttacagcaaa
     4561 atgtataatc cgcaatcttt tgatacattt attaaataca cagataaact ggacttgaaa
     4621 aaacttgtca cgggtttgat tgggacaact ccagataaag tgatcgtaac tgatccagtg
     4681 tacttcgatc atttgaatga catcctgaca gaagctcatt ttccgttatt caaaagctgg
     4741 atgatcatta aaacagtacg ttctcttagt ggttatctat cagaagaatt ccgccaagtc
     4801 agcggaatct attctcgcgt attgtctgga acagacgaag caatgcctca gaaaaaagca
     4861 gcgtattacc ttgcctctgg acaattcgat caagtagttg gcgattacta cggaaaaaaa
     4921 tattttggcg aagctgctaa aaaagacgta gaacaaatgg tgaaaaaaat gatcgccgtt
     4981 tataaaaaac gtctagaaga aaatgactgg cttggggatg caacaaaaga aaaagccgtc
     5041 ataaaattaa ataacttagg tatccaagta ggctaccccg ataagatccc agcattattc
     5101 gaccagttca aaacagtacc tgcagatcag ggtggtactt tgctttcaaa tactcttgtt
     5161 ttcagcaaac tcacattaaa agatcgtttc agcaaatgga acaaaccagt ggatcgttca
     5221 gaatgggaaa tgagtgctga cacggtcaat gcttattatc atccatttcg gaatgtgatc
     5281 gtctttccgg cagcgatcct tcaagcacca ttttacagtc tagaacaatc aagctctgct
     5341 aattatggtg gaatcggagc tgttatcgct catgagattt cccatgcatt tgataataac
     5401 ggcgctttat ttgacgaata tggcaacttg aacaactggt ggacagaaga agatcttaca
     5461 caatttcaaa acaaagcaca agcaatgatc gaagaattcg atggtctgcc ttttgctgac
     5521 ggaaaagtaa acggaaaatt aaccgtttcg gaaaatattg ctgatgcagg cggtctaagc
     5581 tgtgcattgg aagcagcaaa aacggaatca gatctttcta tcgaggaatt ctttgtcaat
     5641 tgggcaacga tttggcgtac aaaagcaaaa aaagaatacc aacaactttt actgcaaatc
     5701 gatgtccatg cccctgctaa actccgagca aacgtgcaac caaaaaacct tgacgaattc
     5761 tatgaaacat ttggtatcac ggaacaagac gctatgtatc tagcgccaga aaaaagagtg
     5821 cgaatctggt aagaggttgt gccagaagtg gtcagcttca agaaataaga aggaatttgg
     5881 gaaaatcgct tctcaaattt ttgtgacatt tcggcttatt tttcgaagaa gttacttctg
     5941 gaataccatg tattagaggt tgtgaaaaag ctgcttagtt tcaagtagta agaaaaattt
     6001 tgtgaaaatg gctttccaca tttttgcata aatttgactt actaccgaga aactggcttt
     6061 tgaacaccgt ttatcgagag gttgtgaaat cagcgtcctg acctgagtat tagagaaaaa
     6121 aacggaaaaa tagttcctca tattttgccg tttgttgagc taataccgag ggtcagctga
     6181 ttgaacaccg tttagtggag agattgtgaa aaagctgtcc tgcatcaagt aataagaaca
     6241 gccaatcaaa agcagcaagg agaacctcat ctataaaaag atagaaggaa gtgagacatc
     6301 tattggtctc atttccttct atttttactt tcctcagtca attgttttac atcataaaag
     6361 gagaaattcc acttattttt ttcgctgttt catgacataa acaactacac cgattaacaa
     6421 aattcctcca gctaatgcta gcaacgggtt attaaattct cccgtttttg ggagtccttg
     6481 agtgccattt ttttcacttt cttttgctgt cctatccgga tgagaaggtt ttccatttgg
     6541 ttttccattt ggtgattcat ttcctggatt ttctctttct ggtttgtcat ttcccggggg
     6601 cacatcggca gtttctcgca cagtgatcgc tttttgctgg gtaacagatt ctgtatgagc
     6661 agaccatttt tccccatcaa atatttccga acgataagtg acttctagcg tatattctcc
     6721 ttttgaaggt gctgaaaaaa tagcggtatg attagaaaat tgagcacttg actgtgtttc
     6781 aacgatttgg atcttttctg ggaaataacg catatcttca aagacaggcg ttccttgatt
     6841 tggtgtgaaa gttgacacaa aaactgtaaa aggcttcagt tcaatgatat ttccttctgc
     6901 attctccgcc acaccatttg cttcttgtat aataatcgga ttattttgtt catttggttt
     6961 tgtctgattt ggttcttgta cgattgcata ctctggatat tccgaagacg gtgaggtttc
     7021 tccgtcaaaa atagcaccta cagataatgg aagaccatcc actctttcct tttctgtttg
     7081 acgattcccc ggtttaatct gcatcagtcc atgaacatgg atacttccat tttcatagcg
     7141 cgtaacaggc tgctgacctt ctttcgaaca tgtatttaga gactcgatcc aatcttttga
     7201 tatggtatgt ccaagagtat tttctgattg tttctctcgt tcattccaat agtagttcaa
     7261 cgagtggtag atttgatctt cgttttgatt caatgggcgg atctgctctc tcatctcaag
     7321 attctctgtt cggaaagata agataccaga agcatggtag tctgtgtttc ctgctgaaga
     7381 ggtataaaga gccacattat atgaaccgtt attaaaactt gtactgctat tgacgataat
     7441 atctgttccg ctattggaat cgattccttt cgcaagattt tcgaaagaca aactattgat
     7501 caattcatga gggcccgtta aagaagaacc gcccattttg aatccattac cgttaccttc
     7561 tccctcaatt cctggtaccc agccgtttcg ataagcaact gagtttttga tgattacttt
     7621 tccgatcgaa cctgtttcat ttttagcaaa aaggtcccaa ccgtcatctg cattatgata
     7681 cgcgatacaa ccgtcaaata catttccttc tcctattgtc agtttggccg caaatccatc
     7741 ggcatcttca aatcctggat cggcattttc aaaagatgta cagttcttga ctaagttatg
     7801 cgcgggccat gcttcgaaag gatcatttga gctaccgctg atttggattc ctgtatttcc
     7861 attccgataa gcatgaatct cttcgatcaa attatgattt ccagaaatct gtaatccttt
     7921 ttgcatagct gcactatttg taatgtcaaa ccctcgtaaa ccccagtagt gactgaacag
     7981 tgtcatccca tttccagttc ctttaaaatc aagtacagga cgcttgtttt ctggattttt
     8041 ctctgcaatc aactgaatcg ggttttctct tgtcccgtta acgccttttg gaatcgtcaa
     8101 tcctctttcg aaggaataag ttccaggcgc catgacgatt gtctgcccag catatgcgta
     8161 tctcaatgct tgtgtcagag ataatggatt ttgtctgctt ccgtttcctg catctgaccc
     8221 ttctggtgtt acataaactg tcgaatattt aggtttacgg tagtccactg tatgaagaat
     8281 ctcttttgtt tcataactag tcatctctac gtattcttct ggcggatagt ttttatctgg
     8341 cgtaaaactg aaagtaaatt cgttcgttcc ttccatcagt tttgtctgat aatcaaattc
     8401 ccgattcgca gtgatagcca agttttttgc gttcttaagc acctctcctg ttgctttatt
     8461 tttaattgtc aataatccgt cagcatttgc tctaaaggac atagtatgat cttggtttcc
     8521 tgaatgatta ctagaggtca cagtgtaaat cggatcaatc aagcgagtag gtctttcttg
     8581 cccaggagga tctgtcgcag gatctgagta agcaacttcg attccttcta ccttgatcgt
     8641 catattcctt gctgcagcaa atcctgcata aataaccgat tcatctgaag cgtataattt
     8701 ttcccagtca tacatgatgt cagaagccat ttcttctcct gtttcaggat cgtaataata
     8761 tgcctctaaa ccagtattcg tttttttcat ctctacgtcc agtttagtaa tcgagttcgt
     8821 ggccggaaac agattcccat gaccgtttcc gaatatatta taagaacctg tttctttttc
     8881 caagaaccct gctgaagtct ctaaaggtgt tgtttccact gagacactcc caggagctgg
     8941 cgcagcttgc ggatcttcag atgatattcc tgtgattgcc cgtgtcccca atccaagtcg
     9001 catcgtatat ttgctgccgc tcgtgttgac cacttcttgt ctatccgagt cccagacata
     9061 ttcgatccga ctgcctagta tggcaaatga atttgaataa aatgcctgcc caaacaaatg
     9121 attttttggt actgcgtctc ttaccattaa agcaaatcct tcttgggcat tcgtataggt
     9181 ccaagattct atttcgatcg tcgcccgcat cctaaagttg atgtctttgg ggattgcttg
     9241 ataataatag gtcattccat ctgatccgct tgactggaat tttccgccgt tattcgtgga
     9301 agagagccgc acgccgtctt ctaatggttc aatggcatac ctactagcac ttgtgctttc
     9361 tccgaaaaca acccctttcc actcttcatt tgcttcttca gattgctttt ttgtcttttc
     9421 attttctgat tctttcatca taggatcatt tccttcttcc gtatccatat cttcactagt
     9481 tcttggtatt tcctccattg ttgacatccc ttcaatgttc acgtgtgcat tttcttcgtt
     9541 cacagactgt tgttcgttag cagcaacttc aacgacagaa attaaaaccg gggtactgag
     9601 cataaggaga ttcatcatta gcgcactgta ttttatacgt ttttttaaca agtgtattcc
     9661 tcccgtaaca aattagaaag cgtttactta aagtattacc taatttattt ttactgtcaa
     9721 taaaaaacaa aattataata agtgtatttt ataaaaagaa aaaatataac ttaagttgtt
     9781 gataataaag agatttatta acggaaaaag aataagtttt tttaaaataa cgtataaaaa
     9841 agagcataaa acaaagtcgt ttgttttatg ctcttctttt attattttgt gctttttatt
     9901 ttatcaaatc actttgtttt tttataaaat ttctgcgcct agtgtggact caaaatgtct
     9961 caatgcccat tcatgtccaa cgggatcgaa acttgccacg gaatctttga aaatatggag
    10021 tttgtatcct agattatacg catcgactgc tgtatgcaac acacagatat ctgtacatac
    10081 gccggtcaaa taaatatctg tgatctggcg ttctcgcaaa cggatgtcta gatctgttcc
    10141 actaaaagcg gaataatgcc gtttgtctat ccaataaaca ttttcctctt gctcatagtc
    10201 ttggtataaa ctttttaaag agccgtacaa ttcccgtcca cccgttccta tcacattatg
    10261 cggaggaaat aagcggttct ccggatgaaa ggaatctttt ggatcatgcg catcaatggc
    10321 aaaaacgaca aaatcctttt gatcaataaa cttctttgta tgagcagtca gctttttttc
    10381 aattacttgt cctgctgtcc cagtagtcag ctttccatcc tctgcaacaa aatcatatgt
    10441 gtagtcaatc gagatcaatg cgttcatctt agtcctcctt atacgagatg tttgacaaat
    10501 tcgatcacca tttctgctga tcgtttgcct gcgtcttcga taaattcgtc aaagctttgt
    10561 gtcgccgagt gatctgctgt gtcgctcatt gcacgtacga tcaaaaatgg gatattgaac
    10621 tgacgagctg tttgtccaac agcggctcct tccatttcgc aagctaatgc ttcagggaaa
    10681 ttcgttaaga tttctttgat tttatctggc gagtcgacaa atgtatcacc tgtaacgatc
    10741 aatccttctt ttgcgttcaa attcgttgct tttgctgctt taaccatttc actgcgcaaa
    10801 tatgtgcttg cttcataata tagaggcatt cctggtaatt gtcctgggtt gtagccaaat
    10861 cctgttgcat ctgcatcaaa ataagctact ttgtctgata taacgatgtc acctacttgt
    10921 aagccttcac cgattccgcc agctgaacct gtattgatca ccatgttcac gccgtattgt
    10981 tggatcaaca gacttgtggt aatagatgct aagacttttc ctataccgga acgtacgacg
    11041 atcacttcgt ggttacctaa tgaacctgaa ataaataaag cgcctgcgcg ttcccatgat
    11101 aatggttcgc ttaggttctc tctcaaaatt tttacttctt cttccattgc gccgataata
    11161 ccgattttca tctttttaat tccttctttc tatataaatg cgattgctaa aaaaaccaca
    11221 cataacatta ggataacaat aataagagat ttcatcaaaa aagagttcca tttggtcgtc
    11281 ttttcccgtt cgctgatcct tgttttcgtg attggtttat tctttttgct ttctttacga
    11341 taaaagctgg caatcttttt ttcttcttgc tgataagctg tttctgcttt tcgctgtttt
    11401 tttaatgact cgcttgcttg tgcttgttgt cgcttgcgaa gttcgcttcg cgtaataagc
    11461 ggtcctttgg ccataagccg atccctctac tttcctttgg tcaatagttc ccagtattga
    11521 atcgcataaa tcgtctttgc atcacaaatc aactgatctt tcatcgcttg ttgcgcctct
    11581 tcaagagtta gatgatacaa ctccagtact tcgtcttcat cttgtgctaa aggattctcg
    11641 accttctcta ccccttgtgc atgataaata tgaaggactt catttgcaaa acctggtgaa
    11701 agatacatcg aagtaagatg gctcagcgac ttcgcacgat agccagtttc ttcttctaat
    11761 tctctagctg ctgtcatttc gggtttttgt ccttctcctg gatcgatttt tccagctgga
    11821 atctccaaaa tcactttttc taaaggtttt cgaaattgtt tgacaagaag cagacgatct
    11881 tgttcgtcaa aagcaatgat cccgacaccg ccaggatgaa ataccagttc tctcttcgaa
    11941 gttcctccat ctggcaaacg aacttcgtct acagcaacat caataatttt tccgctatag
    12001 atttctttgc gactgattgt tttttcttca aattgctcat tgttcagcat atcttctcct
    12061 gctttctttg taatcataac tgcttttttt tcatttttca atttttctca aaaactcctt
    12121 attagtttac catagataaa ccttagattt tctctaaata tagaaaatcc ttctttagtc
    12181 cgatactctt tctagtagtc cgtatgctaa aataagtatg taatcgaata acgaaaagaa
    12241 ttcaatactc aacgataaat gacatggagg gtttattatg ataaaacgaa attggccgtg
    12301 gcttctagca ttggtcttgc tatttctctt tgtccgaaac aatagttttt cttttgttct
    12361 aggactttta ttgataggtg tagttttagt tttgtgggga ctctttggtt cgcgcaggaa
    12421 aaagggaaaa caagaagaaa ttcctgcact gactgacgaa cttgaaactc actatgaaaa
    12481 aagcggtatg acagcgaatg aaatttcatt ttttcgacaa acaatgaatc aaacaaaaaa
    12541 tgagatcaat caattgcaaa tgaatatgca gcagacagcc aaacttaaag caatcgatct
    12601 ccgccatgat ccagtcaaag cagcgaaagc tttattcaaa gaattggtga aagaaccaaa
    12661 ccgactgcat gaagcaagtc agtttcttta tacgcatctg ccgaatctag ttgatttgac
    12721 aaataagtat aatgagatca atgaccatga gatcaaaaac aaacagacct atgaaaaact
    12781 agaagaaagc gcgcagatca tcgaccagct gtcagcttta atcgcacaag attatcaaaa
    12841 attcgttgct gaggatctag atgatatcga cgtagagata tctgttgcga aacagagctt
    12901 aaagcgtgat aataaatatg aaccagaaca aaaggattaa ctcttttgtt cataaaagaa
    12961 gactcggaac attcagatga tgtctggctg tttcgagtac tttttctaaa gtagagacat
    13021 tcatgtaaga atcgagaaca gattcaacaa ggaggaattt tttatggatc ataaaccgac
    13081 tgaaacaaaa gatgttacac cagttaacga tacacttgaa gatttattga acaacccgtt
    13141 ttcaactcca acagatacgc tgaccacttc acaacaagcg gagattgatc agctccaaga
    13201 gcaacagaca gctgctcgtt taattgacaa actaccggcg gaacgccaag aacaagctaa
    13261 acagctagca gcaaaaatcg atgctaatga tgcacagtct gtcatcagct acggatcggc
    13321 tgcccaagca aaattaagtg aattctcaca atcgatgttg aatcatgtgc aagcacaaga
    13381 tattggtcct gtaggagatt ccttaactga actgatgtat cgtctccaag aagccaatcc
    13441 tgacgaactg cgtgccggag aagggaacat cttccagcgt gtcttcggga aggtcaaaca
    13501 atcgatctat gaagtgacag caaaatatca aaagattggt gcacaaattg acaagatttc
    13561 agtgaaatta gataaagaaa aagacggtct gctaaaagat aatctgatgt tagaacagct
    13621 ttaccagaaa aacaaagact actttgatgc actgaatatt tatatcgctg ctggagaatt
    13681 gaaaatggaa gaacttcaaa caacgatcat tccagaagcg atgaaaagag cagaagaaac
    13741 tggcgatcag atggatgttc agattgccaa tgattacacg caattcctag atcgtctgga
    13801 taaacgtacg catgatcttc gcttagctag acagatcacg atccagcaag caccacaaat
    13861 ccgtttgatc caaaacacaa atcaggcctt ggctgaaaag atccaagcat ctatcgctac
    13921 agccattccg ctgtggaaaa accaagtcgt gatcgcattg accttgcttc gtcaaaaaga
    13981 tgcagtaact gctcaacgcc aagtctctga aacaacaaac gacttattga agaaaaattc
    14041 agaaatgctg aaaatctcag ccatcgaaac agcgaaagaa aatgaacgtg gaatcgtgga
    14101 tatcgagacc cttcaaacca ctcaaaatga tttgatcgaa acaatccaag agacattgcg
    14161 tatccaaaaa gaaggaaaag aaaaacgtcg tcatgcagaa gtagagcttg gacacatgga
    14221 agaagatctg aaacaaaaac tgttggattt aacccaatag atcacctttc gcttgttact
    14281 aaaacaaacg cccaacaaca gtttattaca actgttgatg ggcgtttttc ttatctttct
    14341 tctaacttct ccattggagt ctatttctca ttcatcttgc tgtgtccata gccatagcca
    14401 aaataaatga tcagtccaat aaccattacg ataccgaaca cgatccatgt cacagtttgc
    14461 agacgaagca tcaatacaat acaagtcaag atggagacga ttggcaatag cggaacgaat
    14521 ggcactttga attctccagc ttttggtttg ccaaacattt ttctcaactt cagtaagcct
    14581 agactaacaa gcatcaaata ggcgagcgcc atgatattcg ttagttccgc taatacattc
    14641 aatgggaaaa atccggcaaa gaatacagct gccacaccag cggcataagt cgcttttttc
    14701 ggtgtacgat gcttttgatc gatttctgtc ataaacttag gaagtaagcc atccttgctg
    14761 acagcaaaca gtaaacgagc aagtgaatac atcatcgaga tcgttaccgt cagtagcgtt
    14821 aaaattgccc ctacagcgat caccgcgccc actgttccat gtcctacata cctcattgca
    14881 aaagccactg ggtcattgac tcctagttca gtatacggaa cgattcctgt caagatcagc
    14941 gtcacgatga tgtaaaggac agtagcaatc agtatcgaac caatgattcc tcttggcaca
    15001 tctttttgcg ggtttttgac ttcctcggct gccattgcaa cggcatcaaa tcccaagaaa
    15061 gcaaaaaaca caagagctgc accatctagc acaccggaaa aaccaaaagg catgaatggc
    15121 tgccagttat caggtttaac gaaaaatatt cctacaatga taaacaacgc gatgatacca
    15181 aatttgacat ataccatggc attattcaaa cgtaatgctt tcttggcttc aagcgacacg
    15241 atgtaagtga cgaatatcac tacgagcacc gcaatcaagt caatgtatgt accattttcc
    15301 ggattgtacc cgccagttaa tgcttgtgga agttcggccc ctaaactttt caaaaaacct
    15361 tgcatatagc cagaccatcc ggaagcaaca gaggaaaccg ctagtaaaaa ttccccgatc
    15421 aacaaccacc ctgtcagcca ggcagcaaat tcaccaaata caacatacag ataggcatat
    15481 ggcccaccaa tcactggcac tcgcgaagca aattctgcga aacttagtcc agacaaaatg
    15541 acaacgattg cagctaagac aaatgaaagt gacagtgctg gaccagcatt ttgattggcc
    15601 gcaacccctg tcaccacaaa tattcccgta ccgatgatcg caccaattcc aagcatgatc
    15661 aaatcagacg tttttagttc ttttttcatc tcactcgtat gattgagatt gatctctttc
    15721 ttacgaaaca aatccattga agttcctccc cattttaggt tggaaaacag cgattaaact
    15781 gttttcatat accctaaaag gataccacct aaaatgacta aaaggcaacc aacaataaca
    15841 taaatgagct cttttttcgt ttttctttcg ccaaggatga aaatcccacc taaagtagag
    15901 ataatgatcc ccatttgtga aagtgagaag ccaacagcta aaccaagtga tttgactgtt
    15961 agaagcatgc agatgtttcc taagccccat aataatccac agatcatgtt tttccacaca
    16021 aactgatcga ctttaacttt acggaaagca aagaagcttg cgcctaagat cattccgata
    16081 ctttgaggta agataattga catcgcatcc aaattcgccc agttgacgat gatggtatat
    16141 acaccataac caatcgtaga gaagatcaat gctctaaaac ccttaccaaa atcaagcatt
    16201 ttattatctg tatctatttt tccttcgtca tcttgtcggg cagtcaaata cgcaccgctt
    16261 acaagaagag ctaaagcaat aataccgtat acataatctt tcgtttgtgt ccattcatgg
    16321 aagaaaacgg ctcccgcaat cgtattcaaa attaattgaa aaccagtaga caatggtagc
    16381 cctacagaaa cacccatgta cttcattcca tggaactgtc cattttgtcc aacactccaa
    16441 gctaagccgg ataaaaatcc gatcaagaaa atccaagggg tgattacagg actaacaacg
    16501 aaaaagacaa ctaaagaaaa cagaaatgca ccgatcgtca ttcctagtgt ttgttgattg
    16561 gcatcgccac caattttacc acttactaaa ccaatactac cccatgccaa catagggact
    16621 aatgctatta aaatttccat aaccaatctc cttttatttc acatgataag aaacttatca
    16681 gcgaaaacgt taacaacaca agcacttttt ttcgtttcta acgattttct aacgtttttc
    16741 attataaaaa tttaagattg ggaaaaataa agtcattgtt gtcccttttc cttcagttga
    16801 ttcagccgtc aaatggatac ctaatttttc ggccagattt tttgctaaat aaagtcctaa
    16861 tcctgttgaa tgtgttttgc taagtcgacc atttctccca gtaaatccct tatcaaagat
    16921 ccgtttcaag tcttccgctg ggataccgat ccctgaatct ttaactgaaa ggctgactcc
    16981 ttgcgcttcc ttttctatgc tcacaatgat gtttccgtga tccggcgtat atttgatggc
    17041 attactaagc aattggttga agataaaagc gatccattta gcatcggtca aaacagtatg
    17101 atcctcacca acgatctcgt agcgaatatt tttttggata aaataatttg cctgactgcg
    17161 tatcacaggc tgaatgattc cttttagtcc atattcttgg atgaggtagt cccgcgagaa
    17221 actgtcgagt cttgcataat acaaaacttg ttctacgtat tcatcgattt ttgataattc
    17281 attttctagt aatgtatact tttgatcgtc aatatcgaac tcgatcgaac gaactagcaa
    17341 tttagaagcg gaaagtggca ctttgatttc atggacccat gagtcaatat aatctttttg
    17401 gtcttgttga ttactgattg ctgattgcat tacttcttgg tgttcacgga tcagctgatt
    17461 gatatattct tctaccagtt tttcttcttc tgtcctccca ccactcaaat aattttgcaa
    17521 aggggaagtt ttttgtatgt cggctaattt tccccaccat cttctcttgc tcaagtaacc
    17581 acccagcaga taaaaaatca gaaagacgcc ctcaatcaaa gctaaataag caattgtgct
    17641 caaatcaact ttgatgtttg gcgccagcca catcacaaag atcgtcaatg caataaaaaa
    17701 cagccatccc aagatcaatg tccactgatc tttcaaatat ttaaaaaaat tcatcttaac
    17761 cgctcatcct ttcagggaac gatatatccc tgtcctactt ttgtttcgat atatccttgg
    17821 atacctgcct gttcgatttt tcggcgtaac cgattgatgt tgactgtcaa tgtattgtca
    17881 tctacaaatc gttcgtcatc ccacagtgct cgcagcaatt tttcccgact taagatcttt
    17941 ccattgtgct tcatcaaaat aaataataga cgatattcat ttttgctcaa atcaatgatc
    18001 tctccgttga tttccatact tccgttgtca acattcaacg taatgccatt gtggctcatc
    18061 atttcactag ataattctgt gtagttgtaa gaacggcgaa gtaatgcatt gatttttgca
    18121 atcagtacat cgatttggaa atgttttgtc acaaaatcat ctgcccccat gttcatagcc
    18181 atgatcatat ccatattcgt attcctgctg gagataaaaa tgatcggtac tttcgatact
    18241 tcacgtattt tttgacacca ataatagcca tccaaaacgg gaagattaat atctagtaat
    18301 acaagctgtg gttcttctct ttcaaagtct gagaacactt gattgaaatc ggtcgttcca
    18361 aaggtttcaa attgccattt ctcaagttcc tccatgatca attgtcgaat gatctcttcg
    18421 tcttcgacta ccatgatttt tgccataaaa aatccctcca tctttcttct tgctactaca
    18481 atatcaaaga agcccttgtg aaacaagttg gcttacctgg aaaatgtcat tttagcaaac
    18541 gttaaaaaaa actggaaata tgcgatttaa tcgcgtttgt ctcttccaag cagcttaaat
    18601 ttattatgtt attcttaata attggtctat tatctgttat ttttctttcg ttgtttttgt
    18661 cttttaaatt ttcgataaca ccaataaccg aaaagcgtta gcactagcaa tatacctaga
    18721 aggagtataa gtaatcgata aagatggtat ttttttgtct gttctatctt tgaagagact
    18781 tcttctgtaa agggaactcg tcttcctgtc actagtaatc ggtgagtgtt cacggtgtaa
    18841 ggtgtacaag tgataagtgt gactagatcc tgcttttctc taattgctaa tgtatttgtt
    18901 tttgttggaa ggacaacaat tttctcaacg acttcataag cgagcatccg accatacact
    18961 tcaagataga atcggtcatc ttgctccatt tcttcaagat ccgtaaagag ttttttctca
    19021 gcaataccgc tatgggcggt aatcacggaa tgtgtactct ctcctccagt tggaaaagag
    19081 gtgccttgca ggactgtcgc tcctctatct aacaaaagat cattcgtttc atcaaataaa
    19141 ggcaagctaa cattgatttt cggaatatag atggctccga tcatatgttg ttcgtaatat
    19201 tcttttcctg gattttggac atttctaacc gcttgctcaa aaggatcttt gacttgtccc
    19261 attcctggga ttactgatgt atgagcaagc gtctgatttt tcttttcatg cactgctaat
    19321 tgttgctcat gtttttcttc tttatcgcgt tcaagctgct tttgataatt ctcgatcctt
    19381 ctttgatcca aataattgct gagagcatct gctaaaaatg gatatgtaaa aactgcagct
    19441 ccggcaagaa acatcactgc cataaataat tgtaataaaa gactgatttg ggtttgaggt
    19501 ctattaccta ttttcttttt cattttcttc accttcttat ttaaaaaaac tgcagcaacc
    19561 tttataaaac cgattgctgc agcttatttt tgttgcttat acttcagact tcattttaga
    19621 tttacggtac cagctgtacg ctccaatcat caaacttatt ccgattagta agaatgccaa
    19681 gagcccgttc ccaccagtag caggaagtga cccgcgtttc atattcggta ctctttctgg
    19741 ccctggattt tctactccta cgatctgaat ttcttcatta tagcttccat aagagatgat
    19801 aaattcagta aaggctgcct cacctgttgg aagtacataa ccttctggcg ccatggtctc
    19861 tttcatttga taggttccat aatctaaccc ttgaacctca aagtatccat ttccatctgc
    19921 gacaaattct gttgcttgtc cgcttgttgc ccactcaatt ctatctgctc ctgattcata
    19981 aacagctaaa ggcgactccc ctttataaaa tattgcgtat tcagaacggt cttcattcca
    20041 caattcgaat cttgcatctt taagcaattc actgcttgat gcatcatatt tttcaaactt
    20101 tcttccacct gttgtgactt tctctggcgg ctctactcct tcttcataat caaattcatc
    20161 atttcgtccg atttcaataa ccgctgtgtt gttatggaaa tcatccgctt ctagttctgt
    20221 ggaaaccttc aaattatacg tgatcgttaa atctcctcca cgtgcatctg ctaatctacc
    20281 taaagtatct actttggcta ctccattttc ttctgtcaaa atcgtcacga caaacccgtt
    20341 gcttactact tctactatat aatcttgtcc ttttgttaaa atcgtctcgc cggctcgcac
    20401 ttctatttcc tgatcataat aagttagacc aactgtaggc gtatctgtga ttttgtaatt
    20461 tttgataaca gcgcttccgt tttcaaggac agaatcgatg aaataaggaa taggtgctgt
    20521 gatcgtgtaa gaagccgttt ttcctctttc tagatcaata taagaaatcg tttcttgttc
    20581 tccagcttca ttaataatcg ttacttgtct aaggtcgctt tcctctacat tcatctgttt
    20641 atctgcttct ctgatttcgt ttttcggata caaatgcaca tctgtattgt atgtgtcttt
    20701 ccatgtagaa ccatcccaca cgccatccac catatcaggc atcatcactg gcatcataac
    20761 aaccattgga gacgcgattc tggaaatatg agctggcgaa taagtttcta agaaaagata
    20821 gactttatct ctagaagaag gtgcttcttc cgtcacttgg acggtagaga ataccgcttc
    20881 tccagcttca ttcgttgttt gtttgtcaat acttgtcagt cgatatttta aaaaccaact
    20941 ttctgtgtgc cgtttttgaa tccattcaat cgcttcggca gaattaagac cgtcttctcg
    21001 ttccggtgtt aaaggattat ctcgaatcaa atcgtaatat acttccgtta cgtcaaacaa
    21061 atcaaaatcg actcctggaa tattttcttc accaaaatcc gtagaaacca atccgctatt
    21121 cggtcgttct tccggaacag atgagaaacc ttttttgtgt aatgtaatcg tcacttcact
    21181 tggtttttgt gcgacttctt cggctttgac tagttttccg ttcccgaaaa tccctattcc
    21241 cgcagtaacg agtaacagta cactcattcc cgtgacaatc atttttttat tcattttttt
    21301 ctccccgttc tttttttatt aataaaacag cacctaacaa taaaagaatt cctagtaaag
    21361 aaatcaacgt tcttgcttca ttcgtttgcg ggaatacgga ccattttcct gattgacctg
    21421 gattgcttgg accactcggc tctttcggtt tgtccgttgt ttcttttacg gtctcgttat
    21481 aaacacgtgg tgttgcggat tcataggcag agactggaac tttcccggat tctaattcag
    21541 ccatctcctg tccgttaact actacatatt gtggttggcc ttttgcatcc acccaaaatt
    21601 cagggatgat gacctcgatt ctcttacttg cttcatccat ctcgtaacct tccacacctt
    21661 ttaattcttc aaaataatag gtgccagaag gcaagaaacg ttgtcctgtc gtaacaaggc
    21721 cctctttatc cgaaatgaat ttgcttacat ttttgtcatt caaaggatca ttgtctgctg
    21781 gtttgatcca ttgatttttc aagtcattag caggagccat atctaaataa tatttccccc
    21841 cttctatcat ctgatataga gcaaaaacag caccttctaa tggttttcct ttttcagttg
    21901 ttcctctttg acgtccatac ttgaagaagt atggatctct tagataaccg acatttttag
    21961 gatagatatg aatctcttgc agttcctcgg ttgattctgt cgggtgataa attggcaaaa
    22021 tagcagcaat tgggatagct gtctgatcga tatcaacatc caatccaact tctggatcaa
    22081 tcgattcttc gaaaatcaaa taaactgcat cccgatcttg gtttttctta ggtgctttga
    22141 tacgtgcaat cccgtctcct aatccgccaa ccgtatcttt cccagttgtt accgaagtcc
    22201 ctatttggac aagcctttct tctgtgatta gctgacggat attgtttctg ttcgtttcag
    22261 cgatttgctg gacgaattct ttaggagtca tgttttcagc agcttttttt tcatgataat
    22321 aatcggtcat gtcgtaaatg gcaaatgtcg ctccatttag tggcaccgtc tgtgaaatga
    22381 tatctgctgt ttcccctgct tcctcagaga tagccaagcc atcattttca tggtattcaa
    22441 attgatcgtt ataatcgata tctcgaatca tccgtttatg caacacgaat tccacgacat
    22501 cttcccctga ttcttctgcc gacatcgcat gtggaagaag gactgctgca agcggtagta
    22561 aaacaagtaa ttgaagcatc aaacacaaca gctttttcat taccttgctc cctccttttt
    22621 agattgtctg acaaacaaac cataggctgc tcctgcaagc gccatgaaga atgcagcaag
    22681 caggatatag aataatcgtc cggatcctcc tgtagcaggc aacggatttt caggatcgtt
    22741 cccgacacta agctgtaatc tgattcgatt tgtctccaaa agttcatatt ctaactcttg
    22801 ctgtagatca ggcgtctctg cttcttcgtt tggataatac gaaatctcaa cttttagtgt
    22861 acccgcttct tttcctggaa ctacccgatc attttctatg tcaggttctt ctcgataact
    22921 aatttctaaa ataaagtaac ctggaagttt tctatagctg tcaggtgact tcacttcctt
    22981 gatccgatag accccttcca acaagtctga agcattgaat agggaagaat cgtttggatc
    23041 tggtgccaag cctccctcga tgatttcata ctctcctgca ctattttgtt tctcaattga
    23101 aaattctgcg ccttcaagac gtcggctatt catactgtca attttcaaaa gttccaaatc
    23161 gattggcgct agttcatctt ctaaggttac ttgtagtaca ccatcgatga tttcatagcc
    23221 ttccggcaag ggagcagttt cgtcttcgat gaccgtatct tcaatgataa acttaccttc
    23281 ttcattcagt gcaaactgga acacttgatc agatagccga tacccatttg gtgcgacaga
    23341 ttccgagacc tcatattcac caatagacag atgcagaggt tggtcgtaat ttaaatcgac
    23401 atacttttct actcctacgg cacgagaata gaagtatccg atcccaggca aacgtttcga
    23461 ttcttctggt tctaacggat taccagtgta tgctcgatac agcgggaagg aagaatcccc
    23521 cactttcctg atatcgaaac ctgcaccatc taatgtatgg ccttcatggt ctaattttct
    23581 aactcggatc tcccgataat caaacgtatt gtaagcagtg aatgctaccc ataaacgatc
    23641 ctcttcatct gttgtatacg tgagatattg ccaattttct tcacctgttt gcttgagttg
    23701 gatagtccct atagcattat cgtaaagtcg caagatccat gtgtcatttt catcaggttc
    23761 tttgaaacct tctgggttct ggctctcaat gattttgtat tctgcatagt tggtagtttc
    23821 ttcactatcc ttataaacaa atgaatctgg ctctccgatt ggatgaataa ctttctcacc
    23881 atccacgatt acatatttcc cgtcttcatc tactgaatag aacgtgagtc tccctttctc
    23941 ttcctctact tcgttcacac cagaaagccc tttcgctact ggatatccga tttccagtcc
    24001 gttaccatct gtttcataaa gttcaaactc tgcgccattg attggcatat tactgccttc
    24061 aattagttta ttgatattca tgtggattgg cttgaattca tttcgcattt ccagtttgac
    24121 atacaactca tcatactctg tgccttctat gttaggctct tgtacaacag aaagcttgtg
    24181 atattctcct tcataatagg aattcggatc tttaccttcc ggtccgccga ctctcgaacc
    24241 agatgtatat tcatctacaa gctcgtagtg acctaacatc tcatcttcgt tggctgcata
    24301 ttttttgatt tggaattgat acgtctcatc tactaactga taacgtggtg gtccagcgat
    24361 ctctctgatc caaaatatgt cgctgactgc tccagtttca tctcctaaaa tcggcatctt
    24421 cgtttcaaag atcgctattc cgtccgtggt agttttactt gtttcgattg gttcggcttc
    24481 cccactctct ggattttggt catataatgc aaattcgact ccagccaacg gctcgccatt
    24541 ttgacccttc ttgctgactt ggaaattacg gcctttatgt tcgtaacgaa tggaagaaga
    24601 tgggaaatac atccgtccag tagtgatatc attctcataa tcctccaggt tctgaatata
    24661 ggccatattg ttcactgtgc aaaaatttcc gtcttgagct cccgcatcaa gttttacttg
    24721 gtacttgatc ctgaccattt ccccttcacc taaagaaatc ggagaaaatt tataagcttg
    24781 gttttcccgt ccgacatacg ttgtcggagt cgccactact tctggtgggg cttgtccatg
    24841 acgttcttca tcccatattt ctggctcttg gtacttcatg ccgttttctc ctggatctag
    24901 atggtaaaac tgaatatctg ccgatctgcc agctccaaat ggtgagacat taaaaccagt
    24961 ttcatcataa agtgtggctt gctggatcgt tctcttgaag gtagaagaga tatcctccaa
    25021 ggctgtaaca atgtcattct tgttgttcgc attgtaatat cgcggtgtta cgccatcttc
    25081 ttctaaatca gcaatatttt tcaatacatt tcgtccaata gcatctagtc gttgtctccc
    25141 gctaacgttt ccatcgattc ctaaaccaat ggagaagata cttggcgggt tctcttgata
    25201 cttgtttttc agccaatgat gataagcaac tgtgttgatc gcagatgact tttcttgcga
    25261 agtaggactg ccattcgtat tgttattaga agaaccatca ccaacccagt gagtcgcttt
    25321 attatctcct tcttttacct ctccataacg atagtgtaca tttacttcat cttctggacg
    25381 gttaaagtca gatgcatagg taactggatg agtaaaccct gcattagaag tattcccaga
    25441 aaactcctca aaatttctga acaaaacgtc attatcttca gcaatcttat cttccatcac
    25501 agtccagttg ctccaagctc cattagtgct cgtacgataa cttgactgaa tcggtgcata
    25561 actgaaggtc ggtgtcccat ctcccacaac gattaggatt ttttctggat ttctattttc
    25621 tccgccattg tctttataca atgtttcata gccattttta agccctagtg taagcggagt
    25681 tcctccgatt ggtgtacgat ttaatgctgt attctcactt cccgtcaaac gattgatatc
    25741 atttgtcaat ggaaagatgt ccgaactgat ttgctcatgg ttattgatag ctgtactatt
    25801 atggtaaaaa ttcaccatac cgattcgaat atccagactc gggtcagaga gcagatcttt
    25861 actcatttcg tttactgcct caatcaacgc agcattttta gtttgactat tggtcccagc
    25921 ggttaattca ctcatactcg ctgacttatc tagaacaaaa acgatatcga ttggactgat
    25981 ttcctttttc tcgcccccga tgacatctag atagacattg taaagggttg ttgcatcttc
    26041 aggatgtaga tcagatgctg tgctgcttgg cagaacaggt tctacccatt ttttgatgta
    26101 cgccccgtta taatccaagt aactgttttc aaaattccgt tcatttcccc aaagctgaac
    26161 ggtttgagct ggaacgtttc tatcttcttc gcctgcagtt gatccattat tagtagcata
    26221 gtcgtaattt cgccagttcg ctcccccgtt tgtataatca ctatcatcga tcaagttctc
    26281 tccgtcatat ggcgagcctt tgtctccttg atagcgttca ttgctttgtg tccaatttcc
    26341 taacactttt gtatagtcat tcgttgtaaa cttcgggtag cgatgctgtt cgttttcaac
    26401 ttctccgcta ggatagatat attcataggg gtcttgcgta gctgttggcc caagacgctc
    26461 caaaataccg ttgaataact ggcgttgaat gcgctggagt gcttgaccgc tgggtacgat
    26521 tggttcgtcc agcttttcca tctcattttc ttcgctacgt tgaatttcag cggaatcttc
    26581 tgtatcagtt tcattttctt gtaattcttg gttatctatt ctttcatcaa ccaccacttg
    26641 aagaggtagt tacccttcgt tcacatcggg gatcttttct gtttcaaaaa tcaatgtgcc
    26701 ggtttctgct tcttttgaat aaacttttcc gacataccat tcttgaccat cgatcgtctc
    26761 gacttttagg tcttctccat cttcatcata ggaggataga cctgttccac gaacgttttt
    26821 tacggtacca atcccggcgg ctgccgcatc gattgctaaa cgtattctac cttcattggc
    26881 actttccttt ttttcaaaag ccagatgcca ttcaagggtt tcttcttttt caatatactt
    26941 cccttcaatt tttgcatagt cctcatctaa gaatacctca tcctcctttg tttctgttga
    27001 ctcagcagct acatccaggg tcgtcatatg atagactgac tggaaatatg gtccaaatag
    27061 caaactgaca gttactaact gcaaaagctt caattttaac cctttcacat ccttatactc
    27121 ctttcaaaaa tatttgtctt ctttcacaac aatcaacaaa acaatgccca tctaattata
    27181 atttaattta attttaattt taaatcaaat gatatccttt ttagttaaaa aacaccaatt
    27241 atacaacatt tattattaaa attaaattat aaaattttat aacgttctca tttaacaaaa
    27301 cttaagttat taagacaaat gaataaacaa ctacgctttc ctcgaaacaa aaaaataaag
    27361 cacaaatgcg catttacgca ctcatgcttt attcgtacaa ttaattcaga taacggcagc
    27421 gcataggatg ccaataacac ttcctaaaat agctcctcct aaaatgtcag taggataatg
    27481 taaaagtaaa aaaatcctcg aataagcgat caaacaagca aataaaaagg ctaatattcc
    27541 tgcaactgga cttaattcga acaacgcagc tactgataaa aaggctagcg ctgtatgtcc
    27601 tgaaggaaaa gaagaagtct tcggtttact tgtgtaaagg acaagccgac gatttttttc
    27661 ataaggtctt ttccgtcttg tcgttttttt gataaaaagc aaaccaacaa aaaaatagat
    27721 caaactgccg ataagaatgg ctagaccata ttctctagta tcaggtatca agactaaaac
    27781 agcagcaagg agcaaccaaa taaaaccacc attagagata ttactgataa atttcaaaaa
    27841 attgattttt cgactatccg ttttccgttg cttggcgaac ttgttgataa tactatcatc
    27901 gattttagca atcaaacgca acatgttatt cctccttttt aagaaccttt tcttgcgtac
    27961 gatggataaa aagcaggact tgaaaataaa acaagaaact gaaacctaat aaagcaaaat
    28021 agattggtcc tgcaaaccag aatgcgacaa gaaggatcaa cgtgcctgcc aaatagaaaa
    28081 gcgcgctttt gaaatcatta aaaaacaaga aaaatgcagt aatccaagaa gcttcttttt
    28141 gttccagttg aagcaagatt ttgtaactaa agagaagcgc gcttaataga agcaaaaaca
    28201 acgtagtata catcaatgta tagccaattc ccccacccca atagcggaaa atctgataat
    28261 cgatcaatag aagaagaaaa taaacacttg ctaaactatc ttgctttcca taaagagaga
    28321 agttctcacg aaactttttc cagaatagtg aaccgagctt tttctcttca ttttttttgt
    28381 aatgataaat catatacaga actgttcgac ttgcagttcc taatgtgaaa acaggcaaga
    28441 cgcacaggaa ccatagtagc tgtaaatgga taagacgtgg aatccattca atgatttcta
    28501 agaaataatc attggctttt ttaccaatcg atcgattcat catccttttg aacctcctgt
    28561 caaatccatc ccttcaatga aataacgttg tgcaaaaaag aacagcaaaa tcgtcgggat
    28621 caagacaagt gttgctcctg ccatcagata attccattgt gtcgttgctt ggttctgaaa
    28681 aacttgcaag ccaatctgta acgtgtattt ttcttgatct tggatatata acagaggacc
    28741 taagaaatca ttccaagctg cgttgaagga attgatcgca atggtaatca aggcaggttt
    28801 cgtcaatgga agcatcaaat gactccaaat atacaaatga ttcgctccgt cgatttcagc
    28861 agcttcgatc aattcattat tgatcgataa gtaaaattga cgcatcagaa atatattgaa
    28921 cgcattgccg aagaacgatg gaacgatcag gggcaaataa gtacctaccc agccgatctt
    28981 ggagaataat acatattgag ggatcatcgt tacaaacccc gggatcatca tcgtagaaag
    29041 aaccaaagca aacatcagct ttttcccagg aaaatcaagc ttcgcaaatc cataagcaat
    29101 aaaagaattt gaaaggacat taccgatcac tactagcaca gtgatgaata atgtatttct
    29161 tgcatagcgc caaaaaggaa aggcggcaat cgtatccaga tagttttccc atacgatttt
    29221 ttctgggaaa aaggtcggag gataacgaac gatttcttcc attggtttca atgaagtaaa
    29281 caccatccaa agcaatggaa tgatcaaaat gattcctatt actgtcatga ggacataact
    29341 tagtattttt ttgtttgccg attcacactg gtctttttga aataatccat catttgccgt
    29401 cacctccaga ataatagacc cacttaggtg caagcttcaa attgacaaac gtcagaacca
    29461 acacgatcag aaacaggatc attgacatag ccatcgcata acccatatca aaagctacga
    29521 acgccttgtt ccacatatgc agattataaa aaagcaaaga atttgcaggt ccgcctgttc
    29581 catttttcgt catgacataa gcttcttgaa aaatttggaa agaaccgatc gtagaggtga
    29641 tcacatcgta aaaaataata ggagtgatta atggcaaagt aatacttttg aactttcccc
    29701 aaacgcctgc tccatcgatt tctgctgatt catataaaga tctaggaaca ttttgtaatg
    29761 tagctaagta aagtagcatt gctcctccag aactccatat tttcatcaaa atcaacgcag
    29821 gtttcgtcca ttgtggatca aacagccaag ctggtccagg gatatggaac cagcccagaa
    29881 atgtgttgat caatcctgca gatggagaaa gcagctgcat ccataagatg tagactgcta
    29941 ctcctgataa gactgccggt aaatagaaag ctgtacggaa tacactgatt cctttgattt
    30001 tttgattgag tactgtggct aaaaacacac tgaaaatcgt cgtcagcggt acattgaaga
    30061 tgacgaaata aacggtgttg tataacgatt tccaaaataa atcatcgtga acgaacattc
    30121 ttttgaaatt tgctagacca atgaaattca tcttggatgt caaattataa tcggtgaaac
    30181 taccaataac tgaaaaaagc ataggcccta gcataaatac caagaacccg ataataaaag
    30241 gcgagataaa taaataggcc catcttgctt cttttttctt tcggatagat aaacgcttca
    30301 aattttcttg cctcctctgt acctagacag tcaaaaatgg attattgatt ttgcttgact
    30361 aagttttcta ctgctttttg tgcatctgcc aatccttctt ttggtgattt tgttcctaaa
    30421 agaatctggt caatctgttc attcactaat gtcgtgtaat ccggagcatc taaaggaact
    30481 ggtgtgatca ccgtattttt aaagttttcg tccgccattt gatagatcat ttttccttta
    30541 tccgttaggt tgtcattatt cactaaattc tcattggctg tcttgctcgc catgatatca
    30601 aaactttttt cgccgaattt ttcttgtact tctggagaag aaagatattt gatgaattca
    30661 taagaggcct caggatcttt tgctccgtat ggtacttcca agacgaatcc gccaccccac
    30721 gaccaatggc ctgaaccttc ttctttttct gggagctgca tcacgccaaa atcaaaatca
    30781 tccggtgcat tttccatcaa actagagtaa taattgatat tttgggcacg catggctact
    30841 aatccggaaa taaatggatc agatacacca gacccaaatt cggcttctaa tttattgatc
    30901 gtatttttcc cataataatc ttgccagtct tggatccact ccaaggcttc cactttattt
    30961 ttagtatcaa tcttgacttt ttcatctttg tcaaaccaac tagtcccgtt atctgcgttt
    31021 agcgcccata catcagcccc taaattccat aatggataaa aaccgatgcg tgtaaattct
    31081 tttccttctt ttttatctag tttacgtgct gtttcttcta attcatccca agttgtcggt
    31141 agatcttgtt cactgattcc agcttcttta aatatctttt tattataaaa aatgacttgt
    31201 gtatctgtat tgaatgggat cgcataagga tcaccttcat attctactgt atcccaaagc
    31261 tgcggataaa aatccttaga agtgtctttt tctacatatt tggagagatt agttgcttgc
    31321 tgtgcttctg ctctttgggc aacggaattt atgtcctgta caataacatc gggtgcattt
    31381 ccagctgtta ttgcagataa agatttagtc cagatgtcac cccatggctg atacttataa
    31441 tttacatgga tctcgttttg tgattgattg aaatcctcaa tgatttcttc gatagcttct
    31501 tttcgtgcac cggatcccca aaaggaccag aaatcaatat tttttcttcc atcacttgtt
    31561 ttttcagaag aatttgctgc acttgaactg gcatcgcttg cattacttga tccgcatcct
    31621 cctaataaaa acaatgctga acttgccagc aaccctgtaa acaataattt tttcatattt
    31681 tacctccaaa ttttaatgta accgatgccg gttttaatat aacgatatca ttttgttctg
    31741 aaaataacaa tttataaggt atgattctaa aaaaattatt aatgatacca atactttata
    31801 taattcgcga gcgctttctt tgaatttttt aatctttcgt agcagaatga gaatgtatta
    31861 aaaatagatt aggaggaatt ttgatgttta gacaccagaa agaattgcag tttgaagcaa
    31921 aaccagatcg tccgaatcca ttaatcgcga aatatctgca ggagctgatc ggcggccaat
    31981 atggcgagat gagtgtagct atgcagtacc tgttccaagg ctggagctgc cgaggagaag
    32041 aaaaatataa agatatgctg atggacattg caacagaaga aatcggccac gtggaaatgt
    32101 tggcgacaat ggttgctcgt ttattagagg gtgcgcctgt tgaagatcaa gaagcagcga
    32161 tgcaaaaaga tccaacaatc ggtgccatta tctctggaat gaatccacag cacgcgatcg
    32221 tagctggctt aggacctcgt cctgctgaca gcgtgggcaa tccatggagc ggtgcctata
    32281 tcattgcgag tggcaacttg ttagcagact ttagagctaa tttaaatgcc gaatcacaag
    32341 gaagacttca agtagcacgt atttacgaaa tgatcgatga tcgtggcgta aaagatatgc
    32401 tgtctgttct ccttgcaaga gatagctatc atcaaaatct atgggaatgt gcagtgaaag
    32461 aattagaaga aaaagaaggt tcaatcttag tcccaagtac tttcccgcgt gacaaagaac
    32521 gaaaagacat tgcttatgac ttctataact tctcagaggg agaccaaagt agtgaaggtt
    32581 cttgggctag tggcaaagca ctggatggcg aaggcgaata tcaatggttg aaagaaccta
    32641 agatcgaagg cgctgcacca aaattagaac cagtaacacc aaaaatgtat gggacacctc
    32701 cattcaagaa ataaatatct acccgtagaa acacccgttt tttgttccac acgctttgtg
    32761 agcaaaactg actaaagtca ttaatcataa ccacccttca aaagggtggt tttctctaga
    32821 gctataagct caaatcaccg gccagcgcct aaagacgctg gctttcatat tttatgttca
    32881 agccatattg cctttgccag ttttgcccac ctcttaaaag gtttatgtac tactccttag
    32941 cccttaaaag gatcgtctta ctctcgtaca ctcagcttat ctaacgcact atcatgtttt
    33001 tcttgttctt gaatgtactt tttgatggtc gcttcatttt gtcctacttt tgtcacatag
    33061 tatccttcca cccaaaagtg tcgataccca aatttatatt ttagatttgc acaatctctt
    33121 gatgatctcc tgaatattgg ctcgatattg attataaatt atttttcttc tatactttgg
    33181 tgtgaataca atgtggtatt tacacatcta ctttgtacgg gctaaactat ttccctttat
    33241 tgctccaccc gcatagcaag tggttttgta tttcaatcgc tcggcgcgct caattaggtc
    33301 taaagacaat aaatctaaaa acccgctgct tcactgacag aaacaacggg ttttcagaaa
    33361 aaaatgaaat gaaaaagatc tcttacggca atctatttta tttttttgat tttttataaa
    33421 tggaaaatgc ttcaagaagt ccaagaaata aagccgtata aaaacaagaa cctatgaaat
    33481 ctttatttat tagatattca acagaaatcc cgataacact agccaacaat atatttatca
    33541 gaagtgtaaa ataaggatgc tttttataga atagaactag tgttgttttc atagactctc
    33601 ctttcttcat atttccaatt aatcacaaat tcacggcatt actagaaaaa ttaaaggggc
    33661 gcagaagtgg aaaaatactc ttcgactttc cctacaaacc aggccatctt gtgtttaata
    33721 actataccaa atctaaggta cctctaaatc taaattccat ttgtggcggt gacctccaat
    33781 ttttaactct tctatctccc atccagtata cataaccttg atacttcgac cacttatata
    33841 tcgaacacac aaatacctat ccattgcagg agtactacct aacttagttg ctacttgcat
    33901 ctaaataaca gctgaaaaga taggacttac tcttagtaaa gtatctgaac tattaatttt
    33961 taaactatca tttttatcct caactacatg cacatctgtt gcaacttcac tttctgtctc
    34021 atccagataa atgattaagg aatcgggaat tgcaatatct tcagctgacg ctggaatagt
    34081 tgagctaaat tagtcatcat atctttatta taaaataacc aacagcaaaa aatacaatga
    34141 aaaacaaact tggaactaat gtatcattaa taaatgttga aaaattcagc aactcattac
    34201 tgtatgattt tagcagccca accaacatat accctataaa agctaataag atggttagga
    34261 atcctttaga atttttactc attcatagac acttccttaa ataactttta ttatagaagg
    34321 tattgtaaat aataatattg acaaaaagtg actcaaaata aaaaaaacct tacattatac
    34381 tggaatgaac tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg
    34441 gtaaagtaat tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa
    34501 aggtactttt acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc
    34561 aattgcacat tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta
    34621 gcggtagatt tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt
    34681 tctcttttca ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta
    34741 tagaaattct ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata
    34801 gtttaaaatg acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag
    34861 ttgttctttt tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat
    34921 gtcgtgaaaa aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa
    34981 tcattcgttt aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac
    35041 cacactagta gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat
    35101 ataaaaatat tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa
    35161 ttttagccat gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat
    35221 caagagattt taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg
    35281 agtaacgtgt cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg
    35341 ttttacggtt aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt
    35401 ttcctacgga gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc
    35461 tttcttctga cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt
    35521 ctcataatac tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt
    35581 aattgttcca tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc
    35641 gttatcagaa ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag
    35701 cattatcatc actcccttaa taaaagtata ctatataaca ctaagtaacg caaaattccc
    35761 ctttgtaaac tatctagaaa attgttttgt ttaaagtata tcttattgga tataaataga
    35821 aataaattgt agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa
    35881 cctatggatc caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc
    35941 agataaaacg ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta
    36001 taaacaaaaa gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa
    36061 actagcaggt gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac
    36121 attggttcaa cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc
    36181 tatttttgta gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca
    36241 acctcttatc tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt
    36301 tagattccaa gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga
    36361 ctcgcagcac gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct
    36421 atagatgcag tatatgcagc ttatgagcat ttcttctgtt ctctttgttt ttgggatctg
    36481 tgctgaagtc tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct
    36541 tctccattac taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg
    36601 ttacccaagg ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg
    36661 cgactgtgcg gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat
    36721 ggctacgata aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt
    36781 ctttttcttc attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa
    36841 ggtctttttt tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa
    36901 tccggtaaat ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa
    36961 cgtcacaaca agatttgacg gaaaaacttc tccagctgct aattctgctg cctgcttcgg
    37021 ttgtttcgtc tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa
    37081 ataacgacca cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga
    37141 agttcgcacc aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac
    37201 aaactctcca gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa
    37261 caaacatgag aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct
    37321 tacgacctct gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg
    37381 caaaaatgta gaaagctatt ttcacaaaat ttttcttaat actcgaaact aagcgacttt
    37441 ttcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc
    37501 aaacgaaaaa tatgagcagc tatttttatt tggctgttct tattacttga tgcagggcag
    37561 ctttttcaca acctctccac taaacggtgt tcaatcagct aaccttcggt attagctcaa
    37621 aaaacggcaa aatatgagga actattttgc cgtttgtttc tctaatactc aggtcaaagc
    37681 gctgatttca caacctctaa tacacggtgt tccagaagta gcttcttcga aaaataagcc
    37741 gaaatttcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg
    37801 accacttctg tcacaacctc taattattaa agaacctttt gcgaagttaa caatctttct
    37861 ttgatcaatt gaaaattctg cctaagtagt gatagatcct gtgtgacttt caacaataag
    37921 atacttccct ggatttcagc aatcaacgct ttggcttgac ggtctgcttc ttcatcggac
    37981 agtttttctt cagaaagtgc caggatattt tgtttcgtat acttgaccca ttgattgact
    38041 aattctagta gcaacagacg gtattcttca tccactgtac tcaactcaat aatcaaatta
    38101 cctacaggac agccatgata agtgatctgt gtctcatgat cagcgaaaat ccagtccaac
    38161 atttcttcta atgcttcttc tggctgtttt ccagcattta agatgtcttc gaacaattct
    38221 gtctgccata cgtccatatg cgcatggatc accgctagac aaacttcttt ttttgaagaa
    38281 aagtaataat aaaattgacc ttttcctgta tcagaagctg ctagaatatc tcggattgac
    38341 gtttcatgat agcctttttt ataaataaga tctttggtcg cttccaagat cttttccttt
    38401 gttttcaatg atattctcct ttttcatttc tcatagtcct atattataca gtaaattcat
    38461 cattttttct tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac
    38521 tataaaactg ttctcatata gctagatgtt cattgattca agaactgcga ggacaaaagc
    38581 aattcctatc ttatgatagt tgctctaata cttcctctgt tgtcatcact tgatctgcaa
    38641 taaagccaaa gttcgtcaac gctgcatcca ttgcttcttg gccaggagca ccaattgcat
    38701 ctgtcgctac cacaacattg aatccactct ctaccaattc acgcatatgg gaatcgctac
    38761 acaagtttcc gttcattcca ccaagaatta ctgtgtcgat tccatttttt ctcaattgga
    38821 gcgccaaatc attgctttct ggtccaaaga ttttatgagg agagctgatg atcgtatttt
    38881 catccaaata aggtttcaat tcttcgataa aatcggctcc tgaaccttct tccactgatt
    38941 catattgact ttttctagca aacatctgct gctcaagcat catatgttct cctgcaccgc
    39001 caaattgcca tttatggtca tgtgggtaaa aataatgagg agaaataaag agtttgtatc
    39061 ctttttcttt tgcttgtttt accatttcaa tcatattttc gatcgtattg tatttttcta
    39121 agatatcttt tgtcaaacca aagccaccgc ctgctggttt caagaactcg acttgcgggt
    39181 cagtgagaac tactgctgta tttttattat tgaacatggt ggattctcca ttcgttattt
    39241 attttaatac atcatttaga gcttcactca ttgtcggatg agaatagatt tgatctctca
    39301 ataacgtata aggaagtttt ccattcatag ctagcgaaat caagttgatg acttcatgag
    39361 aatcttccgc atagatactt gcccctagaa tcaagtcagt ttcagggtcg actaaaactt
    39421 tgaatacacc tttaggatct tctaatactt gtgctttagg gacaccgctt gtcatatact
    39481 tgaataattt gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa cctacattgg
    39541 ataaagtcgg agtgataaat acactgtaag gcacagcttt ccggttattc gttgttcgtt
    39601 tattctcgcc cttcaactga tccataatga ttcgataatc atctagagag atataagtga
    39661 actgcaatcc gcctttgacg tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt
    39721 attcatccac agcgattgct ccgcgatctg ttagtttaac atctgtattt tctaatccaa
    39781 gtgatgctgt acttggtttt cttccagttg cagccaaaac ttttgacgct tggattgtta
    39841 cttcttggcc atcttctgta aaggtgacgg cagctgttga ttcttggtct gctacttgat
    39901 ctacagatac acctagatgg aattcgattc ccgcatttgt catgtcttct aaaatcattt
    39961 gcgcgatatc ttcatcttca cgtggaagaa attcagaatg ggcatcaaga acgacaactt
    40021 tagatccgta ttcattgaac attgatgcaa attccaagcc aatatatcca gccccgatga
    40081 tcactaattt ttccggtaat tgttcctgat ccatcgcttg cgtagaatca atcaagtagc
    40141 gacttgtatt caatcctttg attggtaaaa tgattggttc agaaccagta ttgatgaaaa
    40201 tccgttcgcc ttcaacaatc actttttcac cattattcgt cacttcgatt tgatggttag
    40261 aaagaaaacg tgctgtaccg tccatcactg tcccagttgc ctcatcgctg atcatatgat
    40321 aatttttggc gcgaagcatg cccgtcaatt tttctttccg agcaacagcc tctgtaaagt
    40381 cgattcctct ttctccattg aaaatcaatg atttagaagg aatacagccg atgttgatac
    40441 atgttccgcc atacatacgt gttgactttt caacgactaa aaccgattca ccttttgaag
    40501 ctaacgcctt cgctaaagtc ttaccgccct ttccaaaacc gatcacaata ttttgatatt
    40561 tttccatcat ttttccctcg cttctttttt acctaccgat tggtacaaaa atatgataac
    40621 cgataattgt ctattctcca aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag
    40681 cggtcctttc acgtttttag tctggttcga tttcctttat tcttctaaac aatcgttact
    40741 tctacatcga tatttcctct agttgctttg gagtaagggc agaattcatg cgcatcttct
    40801 gcaatttttt gggcttcttc ttcgctgatg ccatcgatat gcacttctaa tactacaccg
    40861 atctgatagc cgccatcatc taaattataa agagagacac gtgcttttac tgtggactct
    40921 gctgtcattc ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca agaagcataa
    40981 cctgctgcaa ataattgttc tggatttgtt ccttctgcat ggattcctgg aggagtgatt
    41041 ttcatatgca tcgagccatt tggtgcctcg acttctccat tgcgcccgcc atggttgatc
    41101 attgctgttt cgtatgcttt tttcattgta ctgctccttt cgattgacca tttgctactt
    41161 ggctataatt atagtgtatg acaagtaagg cgctttctac aaaaattctg cttattcgaa
    41221 taccagaatg ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa
    41281 cagcgtcata tttgttccaa aataaatagc aatcttttct acataatctt ttatatgatt
    41341 gattttaaga tacatatata ccagaaagtt gaccccataa accaatactg ttaaaaaaat
    41401 aaccatattc gttaccattt tttcctcctt ttgctggtct ccctaccagc actcgattta
    41461 accataccac tttttttgcc gtccaagcaa ctagcctttc aaaaagactg gtttattctg
    41521 ctgttaatga ttcaagatca gaaaaatgtc cgaataattt tgctaatttt actaacgcca
    41581 acttgttctt tccgaattca tcattttcta tctgtaatac tagtaacggc tcatgtaagc
    41641 ttaaacggat caaaaaccag ccgtttccgt atggctcttc tagatcatat cgaatacctt
    41701 cttcattttc cgggtcgaag ttcatccctt tttcttcttc caacagtcgc ttcatttctt
    41761 ctaatatctt gtttccttgt tcgcgtggtt catcgctgaa caattttaat cggatttctt
    41821 gtacttctac gggctgtttc aactctttga tttgttcatc taatgtgata ccttttcttt
    41881 ttaattctgg caaaagcatc aggatctttg cgatgacata tgcaccatca tccagaaaat
    41941 agttttcctt gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta tctttcccaa
    42001 gacggttaag ttctaatgct ttattgatta catttcgata gccggaaata taacgaactt
    42061 ggatgcctcc tttttcttca atgaatcttt gtagatgaga agaagtaggg gaattcgtca
    42121 cgattcttgt tcctggatgc tcttttaata cgattgtact taatactgcg atcaaattat
    42181 ttcgattgat cactgttcca gaagcactga caactgcaga acgatccaca tctgtatcga
    42241 aaatgatccc aagatctgct tcatttgcta aaacagcctg cttgatactt tccatcgctt
    42301 ctttattatc aggatttggg atatgattag gaaatcggcc atctggttcc aaaaattggg
    42361 aacctgttga atccgcacct aagctattca atactttttc agcaaagaat ccaccagcac
    42421 catttccggc atcaacaatg attttccaac catttagtgg tttgctttct agtgttccaa
    42481 tccccttttt aatttttgtg actaaatctt gcgcataagg tgtcaaaagg tctgcttttt
    42541 ctacgcttcc agtttgttcg ctttttattt tttgctgctg tgataggata aaagcaatat
    42601 cttctttttc tgctcctcct tctgaggtaa agaattttat tccattataa taatagggaa
    42661 gatggctagc agtcagcata accgcagcat cgcacgagaa ctgttcaaat tgagtggaca
    42721 taaacatagc aggcgtagta gctaattcga aatcgatgac atgtatccct tgttctttca
    42781 gactttggat caacgcctct ttcagtgctg gtccgcttat cctactgtca tgaccaatcc
    42841 caatcgtcca ttttttattt ggatcggttg atttcttctt tttctttatc cagtttgcta
    42901 gtccccagcc gatctttttt accatgtctg tcgtcaagtt tacttcatat ccttcaactg
    42961 gtaatgcgat tcctcgaata tccgatccat tttgcagcgc tttcatatct ttcatataaa
    43021 agtcctcctc atttattccc tcattttttc cagcttttct gcaaatcgct gtgcttcttc
    43081 ttctgagcgg caaataataa tgaatgtatc tacaccggcc atcgttccga caacttcagg
    43141 gtaagccact tcatctagcc aattcgttac cacatcggca ccatttcgtt cagtcaatac
    43201 gataaccatg aactgaacag actggatacg caaaacctcg cgtctaattg ctgtacgcaa
    43261 acgttcttca tttaaagaaa cagatggctg gttaaataat gtatagcgta ctattttgtt
    43321 ttcattttga ctttttacta acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt
    43381 cacatcggcg cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc caataggttg
    43441 ttctgtaatg atctggcgga tcagtgcttg tcgctgtttc tttttcatat tagacacctc
    43501 tcaatgttga ttatagcaaa agataagtaa tatttcttca tattttggta ttttttactt
    43561 ttattttttg ttttcattca ggttgctaac ctttttctat tttaacacgc aaaatcgtct
    43621 tcattttttc aggtgctgat tttcttggat cgctgatgta aatctctttg tgttctttag
    43681 aagctctttc atagccttct tttgcgatga atgcttctaa tttctcaaac gttgctggtt
    43741 cttcggcata gggcccgacg tgtaagattt gggcacaaag accttcttcc atctttttga
    43801 atactacttg ttctttcaag tcttccggaa gtttgctaga acgctgaatg gcttcttgtg
    43861 ctatcgcttc tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt
    43921 ttttcatcac tggttcatgc aaatactttt cttgcagtcc ccattgtcct tccagtggat
    43981 agacagtata catctgataa tcaggaatca gccaattttt tttaggcgac atgcgtattg
    44041 catagctgac tgcatataga caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc
    44101 cgatccctct gatgctaaag aatgattttt taggaacctc tagtatttgc gggctttttt
    44161 tcatactata gctcttgtct ttacgccatt caattttttc catttttctt cctccgcgaa
    44221 attccattta gttatttatt atcatacctc tttttgagtg acttggctaa tcgtgttgac
    44281 actatttttc agtttgtgct agaataagtg aagtaaataa atcgtacaga taacacgttt
    44341 cggcgtgtcg agagagaggc ggtttctatt tttataatag aaatcactct ctttttttac
    44401 gaaatttttt agaggtgatg taaatgacaa aacaaaaaca gctgctttta gtacctgcag
    44461 taattgcaac agggatgatg tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa
    44521 ctttcccaac tcttatcaga cagtttgatt taacgactgc acaagtccaa tgggtaacaa
    44581 cgatttatct tttgatgatt tcgatcattg ttccgctgtc tacttatttg aatcgcaatt
    44641 ttagtctcaa aaaactcttt ttagcttcaa atctgctttt cttggcaggt ataatgatcg
    44701 atttcttctc tcctaccttc atggtcctat tgtttggtcg attgctccaa ggtgcagcga
    44761 caggtatcgc tcttccgttg atgttccata ttatcttgac atttgccccc ctcaatcgac
    44821 gaggcgcgat gatgggtgtc ggtactttga caacggccat tgctccagca attggcccca
    44881 cttatggagg cattatgact tcacatttta catggaatca tatcttcctg tttcttgtgc
    44941 ccgttttgtt tctttctttg attatcggct ggtttgctat tcccgagatg gcagtacaaa
    45001 aaagtggtta tttggattgg ataagcgttt taggaatttg tcttttgttt agtggaagtc
    45061 taacattttt gagtacgttg ggaacagttt ccagctggat tgctttaatc atcggcggta
    45121 ttggctggtt ccttttttac tatcgaagta aaaaagtgga gcatccactg atccgactgt
    45181 ctattctttc aaataaaaca ttccgcttgt ttttgattgg cttcttagtt tatcagtttt
    45241 tattacttgg tgtatctttt gtcttgccga atttccttca gatcgttcaa ggaagtagtc
    45301 cttttgtcgc tggattagcg atgcttcccg gcgcagcggt tggtgctttg ctttctcctt
    45361 tttccgggaa aatgctggat caatatggtc cgaagaagcc aatttttgct gggctgattc
    45421 tttctttgat tggttggcta gcattattcc tactcatagg acacgcttca gtgggtgtat
    45481 tgattgcttg tcatgtgttt tatatgatcg gtatcggatt atcttacagc aatatgatga
    45541 ccaccggaat gaatgcactg aatgaagaac tacaaggaga cgggaacgca atgttcaata
    45601 ccttgcagca gttttctggc gctgtggcta cttcattagt agcggtcatt atcaattacg
    45661 tccagcatca tacttcccat aactatgaag tgtctactac attaggatca aaagcagcat
    45721 taggcgtctt gttgctgttg cttctcgtaa gctttgctcg attcgcctat tatctgttct
    45781 ttgctaaaaa agcgtagatt ccatatacgg caggtgttgc atctgccgtt tttttgtgtg
    45841 aaaatggttg tttgtaaaag gaggcttttt tatggaaaaa attgccatta tcggtggcgg
    45901 gatcattggt atgaccctag ccagttactt agatacatca aaattcaatg ttgctttatt
    45961 tgatgaaggg acaggacagg caactaaagc tagcgctggg attatctccc cttggttgtc
    46021 gaaacgcaga aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa
    46081 acttataaaa gatttccaac ttgatcaaac gatttacgca caatcaggga cacttttgct
    46141 tcgacctgaa aaagatttag ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc
    46201 gccagaaatt ggaaacatcg aatggctttc acctgaacag acatctcaag cacttccgtt
    46261 attgaaacca gcagtttcac tgcgtatcag tggtggtggt cgtctggatg gaaaagcata
    46321 tttagaacat cttggtcagc gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt
    46381 cactttgcat aagaagagcg attcttggct catcaaggga ccgtttggag aatacacagc
    46441 tgatcgtgta attttgacac ctggcccggc acttaagcct ttattacaag aattaggcta
    46501 tgctgcagat atacgtccgc aaaaagggca gctcttagca tttcagacat cttataagat
    46561 gagtcaagac tggcctgttg ctatgcttga cggagaagct gatttcattc ctttcatgga
    46621 tggaaagatc cttttaggtg ctacccatga aaacgatcaa ggctgggatt tggctcctac
    46681 tagcgaagct tataagggat tgactaagag tacagaacag ttcttgactg atccagatca
    46741 gataaaaaac tggccatacg agttccgtgt aggtacccgt gcttatacgt cagatttttc
    46801 cccatttttc ggcgtactgc cagatgatcc aagcttactg gctgctagcg gtctaggctc
    46861 ttctggcttg accacaggtc cttatatcgg ctatctatta gccaacttct taaatactgg
    46921 tcattggcct acggaagatt atcaaaagga catctccacc tatattacga aaaatccatc
    46981 atagaaaaac agctttttca agtaaggatc ccgagcgata ttgcgcatgg attacttctt
    47041 gaaaaagctg ttattttagt tcaatttctg aatcaaattc tccaaacttt cttttgcttc
    47101 atctggcaag agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg
    47161 ttgtttcacc agatttttat aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg
    47221 taaatagtgg atgtcttcaa atggaccaaa cgacttgaat tctgccgttt gttgcacgat
    47281 tctttcaacg attgacaatg tcgtttcagg tttgattttt ttatcatcga aatatccagt
    47341 attcaggaca tagcaggctg tttgctgttt caaaaacaac tctcgaaaag cttgatagtc
    47401 ttccctaaga ggatagacac ggaaaggatt agcaaatggt tcaatgacta gctcatctag
    47461 ctgtcttttt gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa
    47521 agacgctgct aaaactgggt cattgatctt gataattggc ggaagcgaat catctttcat
    47581 gatccaaaaa acagcatcga tcttttcgct aagatgatcg actcggtcgg gtgtcgcata
    47641 acgtgatttg actgtccgcc catttccgtt tcgtatatct cctgttacta ggactttttc
    47701 tcctttactt gtacatgtca cgcccacatt ctgacaagta aggaaagaag aaatggccgg
    47761 atcatcagac ggatagtctt gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa
    47821 accatttttt cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc
    47881 cgtatgtttt gctaaggtaa tcgttgattt tccagaaccg gacaacccaa atacagccat
    47941 cgtataggtc tggtcattac tagcatattg tttcatccca ccgtggcagg caataaagcc
    48001 attgcgattt cccaatgccc aagcaagcgt caaagtcgct ttttttaatt ctccaaagta
    48061 acgtaatcct agcacagccg ctgtattatg gagagcatca aacaaaatga gtccatttgg
    48121 aaatgtttca tgtgtccatt cgggatcaca aaagatataa agatctccct catcaaagga
    48181 aacagacttt tgataagtct ctaagatctt cgaattcgct ggctgaaaat tcagcaaata
    48241 agaatacaaa ttgttctcaa atccttctgg aatcagcaaa tgactttgca cagaaaattc
    48301 ttcagatagg ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac tttgatacac
    48361 agcttctctt aaaattttct cgtatttgct ttgttccttg tctaagtcct caaagatccg
    48421 ccgagcactt gctgttcttc ccacaatgct tccatcattg aatactagtg tcctaacatc
    48481 agctggtaaa tctagttctt cagcatgtga gattgttaaa tctgtgatga tcgttcctgg
    48541 actccctttt gctagttgat aagctgtctt cctatcaaaa acttcatgaa cattattacc
    48601 gtaaaatgtc gtttctacaa tcgatcgaat actggataac agtggatttc cacttgtgat
    48661 ttcgtttaat tcgagactct cttttgtact catggctcct cctcattccc taaacaaaaa
    48721 aacgttttct ttattataca ttattcgagt ttttatttgt actgctgcgc tgtgggaagc
    48781 aaacgagtta tttttcacac aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc
    48841 atcattttcc ttttatcggc ttagtaccag ccgtttgcta accagaatgt tttagcagca
    48901 tcccatgaac cataacgaga tgttacatat tgttctgcta cacgttcttg attggcagct
    48961 gaatagtcgc cgtttaagta tgaagcatct aattgataac ggccgatgta acgtccgtta
    49021 gtagctgtgt atgaaccgcc tgattctttt tgtgcgatcc attcttttgc agagcttgaa
    49081 gttggcgctg ttgcagcagc tggggcagca ggagctactt caactgtttc aacaggtgca
    49141 acgtatgtat gttcttgcgt tgctgtttca gttgtagatg ctgtattttc tgttgttttg
    49201 ttttctgttg ttgctgaaga atcatttgaa gttgggattg ttaattgttc accaacataa
    49261 atgcggttga tgtttgcgat cgaatttttt tcagcaattg catcgatcat tgagttgtct
    49321 cctgcaaatt tttgtgagat cttagataat gaatcacctg attttactgt ataaacttca
    49381 tcagcgtgag catttgtagt tcctacaaac attgcagtag caccagcagc aaaagttgta
    49441 cctaaaacaa ttttcttaaa tgaattcata ttaaactcct ttttttctcc gttttttatt
    49501 ctttcgacgc tattaacttt atcatttaga aatatttcat aggtaaaaga aacatgacat
    49561 ttaatgacaa aataacgaat tatttaatat ttgtaacatt taaacaaata tttttttgat
    49621 acaaaaccat aaaagccttg ttgtataagc aaagtaatcg tttttctatg tttttttatt
    49681 tttgaatgaa aaacgcagat tctgtaacat tcactgcaat aatccataaa aaaaagcaaa
    49741 aaaatttttg atttttatca aattctctta aaaaaatgta aaaaatacaa aaaaaccagt
    49801 aattgttaca acaattactg gacaaaaact ttgaataatg ttaactattt ttttatttta
    49861 actttctccg caataatttc tgggcatgtt ccaattcgtt tataacgact tcttctgttt
    49921 ctttagctaa tatttcatct aatacatcaa gccatttttc cggttctttt gttcctagac
    49981 gtccaatagc ccaagctgct gttcctctga tgacagggcg gacatctgtt aaacattctg
    50041 aaatctcagg caatgccgtt cgatcaccta gattagctaa agcaatcaat gcgtttctct
    50101 gcaaaggttt cttcccacgc catgaaccag ctaaatgccc aaattgctgt ttgaagtctt
    50161 tattagacat cgaaagcatt ggctttaatt ttggatatat ttcgtctact tttggttcca
    50221 tctcttcatg aaaatgaaaa tcttttcctt gattgtaagg gcagactaac tggcagatat
    50281 cacatccata aatgacattt cgcatttttt tacgatattc ttccggcatc atcccttttg
    50341 tctgagtttg ataagacaaa catcgctggg cattcattct tccgtcacca agcaatgctt
    50401 ttgtcggaca agcagtcacg cagcgcatac agtcaccgca accaaattct cctggtttat
    50461 caggctcaaa tacaatatta gtaacgatct caccaagata gacaaatgaa ccgaactctt
    50521 cagtgatcaa caatccgttt cgtccaataa atcctaaacc agctcgctgt gctacagcga
    50581 catcgatcag ttctccagta tctacttgcg gagctagacg ccattcctct tctttttgcc
    50641 atttttctgc tcttgtttgg atgaattcga tcaatcggtt caaacggtct ctgagtatat
    50701 catggtaatc gatcccccaa gaagcacgag caaactgccc gcgtttttca tccctaggca
    50761 cttcttcatg gatcctagta ggatacgcaa gtgcaatgga aatgatcgat ttcggctgat
    50821 caaatgtcat ttctggataa agacgctcat cgatatttgg atgttcaaag ccagaagtat
    50881 ggccagctgc tttttgttct tccaacgatt cttttaatga atcaaaaggt tccgccgtgg
    50941 tgaacccgat tttgtcaatt cctaatcgtt tgctttcttg tatgatttct tcttttaatg
    51001 tcatggtctt cctcgcttgc tgttaacggc tgtataaccc ataatatagt ttttcttttt
    51061 tcagctggac aaactggatc tgctgatcgg caatcttctt ttcttccatc catttttttc
    51121 ttgcagcaaa tacttcccaa taatttgttg aaattaattt tcttttgtta tctaatgcat
    51181 taaaaaaatt caatgaaact ggttttgtac ttaacagttc atggagcctt acctcttccc
    51241 atggcgaaaa ctcagacaga tcgcgggaat acaatacttc ttcatagaga tttttttgct
    51301 gtgtgtacgt gaatgtttgc gggattttcc caggcctagc aatttctact agacgattat
    51361 aaaacatatt cattaatttg ctgttggcaa taaattgact tttataatag gccgactgga
    51421 tctttttgat tactccttct gctttgatga ctgaaaataa tgcaggccat gctttgacgg
    51481 ttatttcgtc gttttgttta actaatacgc caggtgtgaa gccgactgga gaaaggaaca
    51541 caaaataacc gataccggta ttttcatcta aaggctgaaa ttcatcaaac aaccatttgc
    51601 ctatgtacag ctcttcatct ggttctgttg tctcattttg atttgctatg aataatttag
    51661 ctaattctgc ttcaagaaat tgaaggtctt gatccttgtc ggtattttcc tgctggctga
    51721 tcaatcgaaa gacttcttct ttaatcgtat atttatcttt acgtgtaaaa ttcatagcca
    51781 caccttcctt ccttactcta actataacgg aatttcttca aaaaaggtat ctgtacgacg
    51841 aaaaacagca taattttttt tcatatctct tataaaaaat gcaaggtctg ctcctcaagc
    51901 tagaccttgc acttttcagc tttcttttgt ttgtgacaaa ccttcatgga tcgtttcaat
    51961 attccatttc atcatctgat agtaagaatc tccttgttct ccttcttttg ctatggaatc
    52021 agtgaacagt ttatcataga ttttcaaacc cgtttctttt gctacccgtt ccatgcttct
    52081 tgagtctaca ctggtttcca cgaataaaac cggtacttct tttgctctta tctgatcaat
    52141 gatcgctttc atttgatcag gcgtaccttg actttctgta ttgatctccc atatataagc
    52201 tgctggcaaa tcatatgctc gtgaaaaata cttgaatgcc ccttcacttg ttactaataa
    52261 ttttttgttc tcttcgatag aagcaaaact ttccttcgct tgtgtatcta attcttttag
    52321 tttttccaca tatgcttctg catttttttt atagagtgtc gcattttctg catctttttc
    52381 agagaatata cgtgcgattt cctccacata ttttattccg ttagataggt ctagccaagc
    52441 atggggatct gcttttgtat gttcttcacc gctagttaaa taaagaggtt ctacattttt
    52501 gctaactgca aaataatctt ttccttcttc ttttttagcc gtttccatca agttatcgaa
    52561 ccagctgtta cctgtttcaa gattcaaacc gttgtataaa ataacatctg catcgcttgc
    52621 ctttttgatg tcttctggta gtacttcata ttcatgcgga tctgttccga ctggtacgat
    52681 actgtggata tctatttgat ctgttccgac ctcttttgcc atgtccgcca agatcgaatt
    52741 ggtagccacg actgacaact tttctttact tccttcatcc gcttggtcag aagcctgctt
    52801 tccgcaaccg gctaatacca agaaactaac tgccaagatt aaaaagagtg atttttttgt
    52861 tttcatctat ctgcttcctt tctgaaataa aattcctttt gttggagaaa ataaaaaagc
    52921 aaggataaaa aatgcggtac atgctaacac gatcgtagct cctgatggcc aattgtaact
    52981 ataactgaaa aacacaccga caactgcact tagcataccg attcctgcag ctgtaatgat
    53041 cattttcagt aaatggttcg ttagcaagta agccgtggcg gctggtgtga tcaacatggc
    53101 aatcaccaag attgttccta ctgtctgtaa actgactaca gccactaatg tcaaaaagaa
    53161 catcaaaaga taatggatca gcgatgtgtt cagcccataa gcttttgcca ttgtcggatc
    53221 gaatgatgtg atcttcaatt ctttataaaa gaaaaagaca aatatcaaga cgattccact
    53281 aacgagtgct gtcagcaaca aatcgctttc cctaacagct aagacattcc caaataaaat
    53341 atgatacaaa tctgtcgcac tctgggcata tgaaatcaag ataatcccta atgcaaaaaa
    53401 ggagctaaag acaattccaa tcgctgtatc atttttcaat cggctccgct gagtcacgaa
    53461 accaatcact gctgctgtta gcatcccaaa agcagtggct ccaaagatgt aactaaaacc
    53521 aaacatataa gaaatggcta cacctggaag aactgcgtga gagatagcgt ctcccatcaa
    53581 agacatccct cgtaaaataa taaaagaacc gatgactcca gaagcaagtc ctacaagcat
    53641 ggaagtgacc aaagcgtatt gcaggaattg ataattcatt aatccatcaa taaaggattg
    53701 gatcattttt tctctcctcc ttttactaaa tgtctgatga tctcaccata ggccaattga
    53761 aggttcttcg atgtaaagac ttcttgtact ggaccggaag cgatcagctg tttatttaaa
    53821 agaataacct catcgaaata ttcttctact ttatgaagat catgatggac gatcaaaatc
    53881 gtttttcctg aattcttcaa ttcctgcaag atgtcaaaga tctttcgttc acttaacgca
    53941 tcaatcccta cgaatggttc atctaaaaag atccattctg ctccttgggc tagagctctt
    54001 gcaataaaaa ctctctgaag ctgtccaccc gatagttcgc tgatctgtct tttggcatat
    54061 tcttccatcc ctactttttt caaagcttgt cttgcacgtt ctttttcttt tttcccaggt
    54121 ctttgtccaa ttcgtaaaga tggatatgtt cctaaaagta caacgcctaa tacatcaatt
    54181 ggaaaggaaa gatccaattc acttcgctgt tccacgtagg ctattttttt acggactgag
    54241 tttaccgact gtccagaaaa ggtgacttta ccagtagtag ctggtatcaa ctctagcaaa
    54301 gatttcataa atgttgattt tcctgcaccg tttggtccga taataccggt aattttttgt
    54361 ggatttatcg tcaagttgat agttttcaaa acagttttcc cttgataagc ggctgaaagg
    54421 tcttgaatta caatggccgt acgttccaaa tcttacactc ctttttaggt ttacctaact
    54481 ttttatttaa atatagttta actaaaatgg aaagcatttt caagtagaat gatgaaaaag
    54541 tttttttcga attttcctcg cattttatga aaattttctg tagaatcaca tcaagagttc
    54601 aataaggcta acctaataat gaaaaacatg tgttgaaacg tttgattatt ttttcttgtt
    54661 gactcaacaa cgaaaggagt ttttgtatgg aagaagaaaa gttaactcga agtcttagtg
    54721 cccgtcatat ccaaatgatc gcactaggtg gaacgatcgg tgtcggtctg tttatgggtg
    54781 cctcctcaac catacgttgg acagggccct cagtgatggg agcctatgcg attgcaggat
    54841 tctttttgta tttgattatg cgtgctttag gcgaaatgct ttacgttgat ccttcgactg
    54901 gttcttttgc aaattacgcg agcgagtaca tccatcctgt ggctggctac ttgaccgctt
    54961 ggagtaatat ttttcaatat attgtcgtag gaataagtga agtgattgct gtaggatctt
    55021 atatgaatta ttggtggcct gatttgccgg caattattcc cggaatcatt gtcgtcttgt
    55081 ttcttatgct ggcaaatctt atttctgtca aggcgtttgg cgaattagaa ttttggtttt
    55141 cgatcatcaa agtcataacg atcattttga tgatcattgc aggtcttggg gtaatcttat
    55201 ttggatttgg taatcatggt caggcagtag ggatttcaaa tctttggaaa aatggtggat
    55261 tcttcactgg aggcgtcaaa ggatttttct ttgcattatc gatcgttgtt gcttcctatc
    55321 aaggaatcga actgatcgga atgactgctg gtgaagcaga gaatcctaaa aagacgatca
    55381 ttgaagctgt ccagtccact atcggtcgaa tcttgatttt ttatattggt gctattttta
    55441 tcattgtgag tatctatccg tggaatcaat tgagtgagat tggttcacct tttgtacaaa
    55501 ctttttctaa agtcggcatt acatttgctg caggactgat taattttgtc gtgattacag
    55561 cggcattatc aggttgcaat tcagggattt tcagcgcaag tcgtatggtc tatacacttg
    55621 ccataaatgg aaagatgtct aaaaaatttc tgaaactatc tcgtcacggt gcgccttttt
    55681 atcctgtcgt agcaatctca ggtggaatct taattggtct gatcttaaat tatcttctac
    55741 caatgttcat caaccagtca gaaaatattt tcgttttcgt atacagctca agtattttgc
    55801 ctggtatggt tccttgggta gtgattttga tcagcgagat tcgcttcaaa aagaagcacg
    55861 cagataaaat gcatgatcat ccttttaaaa tgccactatc tccttatacg aattatgtga
    55921 cacttgcttt tttagctatc gtattgattt tcatgtttat caatcctgaa acgagaatct
    55981 cattgattat tggactcgtt ttcttgcttt acatgacgat ccattacttt gtacgcgaaa
    56041 aaagaaccgg aaaataaaaa aagaacgaag cttcacctta gtggaagctt cgttcttttt
    56101 tattgctttt atcaattcaa ttttagcgtc tcaccgggag tcaggaaata attctccatg
    56161 ctcatcccat tcagcgacaa gatcgtttct actggtacgc cagttcgttc ggctatttga
    56221 tttggacctt ctccttccaa aactacggtt gtcgcagcct gtgaagtcgc gctgttggta
    56281 ttagtttcag aggaatctgt tgtgtcttgt gttgttcgag cttgcatttc agaagcttct
    56341 accgactctg ctgtctcaga atcagaagat gttgtgtctt gtgtgcttgt tgactctgct
    56401 tgaagagagg cgctagtctg caaataaata tcggaaatct tagcgacatg attcgtataa
    56461 ttagccgata attgattgat caaagtatct aagtctacac cgtctttatt ttctgctgcc
    56521 tgatacgctg cacgtaattt tctgatccct tcgtcggaga ttgcttgaag ctcacgtgct
    56581 ttttggttag caactgctga taaaccagct attccctctt gatttccctg aatttctgtc
    56641 tggtattctt caatcaatgc aggcgtcttt tcttcgatct ttttcgaata atcatttacg
    56701 attgcttgaa gttgctctgg caaaccattt gctgtttttt cttcagaagt ttgtgtctct
    56761 tttgttttag ttgatgactc acttgtttgt ttcgtctctg ttttctttgt cgttgtttcc
    56821 gttatggtcg tacttttcgc ttctgacgaa ttttttccat gaaaatatag acccgcaact
    56881 cctcctgcta tgatcaaaaa cacggcaact gctgccccaa tcatgatcat ccgcttacga
    56941 gctgcctgtt tttgctgtct tcttcgggtc atccgaacat tttctcccca ctcattttca
    57001 tcatacattt ttttcaccac caatttttat gtataatagc gttatcaaac gcttcatatt
    57061 cttattgtag caaataattg tcgctgtgca catttttatc tttgttattt ataagaaaat
    57121 ttcaccagtt gagatgattc caagattgtc cgtcgatttc tccttgcagc tgcattgttc
    57181 gttcgtctat ctcagccatc agtttcttta tttcatataa gagcgcacgg tcttgatatt
    57241 ctgatttttc gcttaattca tctaattgtt ggatcagcca atcatttgtc ataatgaagt
    57301 tcctttcatt tcttgcagtg taaaggtcaa gtcttcgatc tgctcttttg cctcagtcag
    57361 tgcttctacg atatatgctt gtttttcaga ttcttcttcc tgcattttct gattttcctg
    57421 tactctttga ataaaccagt caatccgctg agtcagcgat ggtttgcctt ccaatgagag
    57481 atactgctga taagcaatta gcttctccca acgtttttcc tgggataaat acataaattg
    57541 tgctaaaacg aatggcggaa gataggtcat atggaattta tttgcgattc gctgatacgg
    57601 cctcaaaaat tcggaaatcg tatattcttc ttttcctcca gcttggtatt ctttttccgc
    57661 cacacctgtt gtcaatacga ttcctagttc tttccctttt aatacatgtg ctgctcgttc
    57721 caagacatta tcttgccaaa tttttaaatg agcaggagaa ctataccagt aaaacggaaa
    57781 ctggaaaatg atccgatcat gctgggccaa gagctgtagt tcagtctcta tatctatttt
    57841 tccgtctgga taacttgatt ccaaatgatg ccaagtagct ggtaaaccag cagaagcttc
    57901 tttgaaaaat tgctgattta aagattcttc gatcgttgga tggctaatga tgataagtgt
    57961 acgcatccct tttcctcctt tttagtcttc gctttacagt gtagcataac cgccacaagt
    58021 ttcttttgcc gatgcttgtc attttaacca gactaatcag gttgtccaag aaactgcggt
    58081 ttgtttttcc aactgttttt tcaaggtatc caaagcaata ttcttaaaat tttcgttttt
    58141 catacgttta cgatagttcg tagccgtttc tccggtactc tttttgaaca ttcggctaaa
    58201 tacgactaga ctattgaacc ctacttgata ggcaatactt tccacggatt catccgtcag
    58261 agaaagaaga aacgcccctc gattgatccg taccccgatc aaatattcct tgatactggt
    58321 acccatatga tttttgaata ctcgcgacaa atggctccga ttgaccgcta aatgctgggc
    58381 gatttcctca acagacaatt tttcagcata atgtgcctcg atgtatttga ctgtttcaac
    58441 agctaaagag gaatgaacct tttgtacacc accgctgacc tttccgccat cttttaaaag
    58501 gactgataaa aaacggtagg ttaatgctgt taactgtaat tcattttcca aggtttcttc
    58561 cgtatctttc atgcattcta aaataatgtc gatatatttt tgtatgtcag aagagaccat
    58621 cgtgtattgg tcttctctga aaagcgactg ccgaatgatt tcttctgcag cactcccacc
    58681 aaatgaaagc cagcaataca tccacggatc ttctccatct gataagtaaa acgtcgagtc
    58741 tcctggacga atcaagaaca gatcaccttt tttcaattgg aattgttgat ttttcacatg
    58801 ataagatcct ttgccttcca tcacgacatg caggatgtaa tcattgcgaa tagcaggacc
    58861 aaaagaatga aacggcatcg ttttagagaa accacaaaaa tcaaaataca attctttttc
    58921 tacttgttga tgttcctttt ttaaaaaaat cgattgctcc acgtctatcc ctccttactc
    58981 ctttagtata atgcaacaaa aagttaaata aaagtcacag aaatatatta aaaaggaagc
    59041 gatttcttgt taagtttaag tcattaaaag atctttgtta ttcgttgtaa ccggtttact
    59101 tagtattata tattgaacag gagaggaata cagattatga aaagcaaaat gatccaacgt
    59161 gtgtcctacg ctttaggcgc ttttggacac gacacattct atgttacatt aagcacttat
    59221 tttatggtat tcgtgacaag ccagatgttc gcaggaacag ataaggcaac gaatgcaaaa
    59281 atgattgcta cagttacaag cttagttgta ggtatccgct tagttgaaat tatttttgat
    59341 cctttgattg gtggcatcat cgataatact cgtacaaaat acggtaaatt caaaccatgg
    59401 ttagttatcg gcggcgttgt tagttccgta ctcttggtag caatctttac caatttcttt
    59461 ggcttagcta ctcataatac gactttattt atcatattat ttgcaatcgt tttcatctta
    59521 ttggatagtt tttattcatt taaggatatc gctttttggt caatgattcc agctttgagt
    59581 acggaaacaa aggaacgtga aaaatttgct acgtttgctc gtttcggctc ttctctaggt
    59641 gcaaatggta ctactctagt cgttgtccct atcgttactt tctttaccta tcttgttact
    59701 cataaacata ttgaaggcgc ttcaggctgg ttctggttcg gtgtgattgc tgctgttatt
    59761 tcaggcggta ctgcgattat cactgccttg ggcacaaaag aagtcgatac tgcgatccgt
    59821 cagcaaaatg aaaaagctgg gatcaaagat gtgttcaaag cagttgctca aaatgatcag
    59881 ttgatgtggc ttgcccttgc ttatatcgct tatgcgattg ccaatgtagc aacaactgcc
    59941 gtattattct acttcttcaa atatgtactt ggccaaccaa ctgaattttg gatcgttggt
    60001 ctagttgctg ccttacttgg tttagtagct gttccactat tcccaatgct tacacgcgtc
    60061 atctctcgtc gctacgttta ccttggaggg atcgcaatga tgatttcttc ttactttttc
    60121 tttacgatcg caggttcaag tttgccaatc gttatcatcg ggctagtatt gttctacttc
    60181 cctcaacaat tgattttctt atccgctttg atgacgatca cagactctgt tgaatatggt
    60241 caatggaaaa atggtatccg taacgaagct gttactctat caattcgccc tttactagat
    60301 aaaatcgctg gtgctttgtc taacgggatc gtagggtttg tcgctatcgc ttctgggatg
    60361 actggtaatg ctactgctgc tgatatcact gctcatggcg taacgacttt caaagcttat
    60421 gcattctatg ctcctgctgc tttgatgatt ttctccgcta ttatttttgc ttggaagatt
    60481 acattgacag agaaaaaaca tgcacaaatc gttgagaaat tggaaaacaa attggctcca
    60541 gctgatacaa tggaagatga acttcacgat gcagtagttc cttcccatta agaaaggatc
    60601 ttgaaaatga agattacaga aaaagatttt ggccaaggtt atcacttgat aaccttggag
    60661 aacaaaaaca agctagcact ttctatctct gatttaggtg ctagaatcgt cagtttaaaa
    60721 tcaaatgatc gagaattagt tttgggattt gacaccgctg aagaatatat cgaaaaagat
    60781 ccgtatatcg gtgcttctat cggtcgcacc gcagggagaa tcgaaaatgg ccgcttttct
    60841 ctcaatggaa aaacttacca attagccaca gatccaaaaa ctggacataa tctgcatggt
    60901 ggtgctcctg gatttgaatt aaaaaaatgg tcttatgtca ttttgaatgg cgaaaatgaa
    60961 gcttctgtca tttttactac tacaagtccc gacggagaac atggttttcc aggtacaatg
    61021 gatgtggaaa tccgctatac tttgacaaaa gataatatct ggcgagtgac ctctcgcggc
    61081 accagtgacc aagatacttt attcaatccg acaaatcatg tttacttcaa cttgacggga
    61141 gatgcttctc agtcaatcga tcaacatgag ctttggctca atagcgaagc ttatgctcct
    61201 ttgcgaacag atagtattcc tataggggta aaagaaaatg ctgcaggaag tgctttcgat
    61261 tttcaaatac ctaaaaaatt agcgtccgtt tttgctagtg atttggatca aaaaaatctt
    61321 gttgacggaa tcgatcatcc atttttctta aaagaaaccg gcttgggtaa agaagccgct
    61381 cgcttgacta gccccgataa aagaatccaa gtagatattg ctacagatgc ttctagtgtg
    61441 gttattttca ctgcaaattt cggcacagaa acaccagaaa tgcgcaaccg aaaactggca
    61501 catcacggtg gcatcacatt tgagactcaa acagctccag gtgcagagcg cttctcagca
    61561 tttggttcga ttcatttaaa agccggatca gtatttgaaa cagtaacaga attcaaaatc
    61621 aaaacaagaa aggaataact catcatggaa atcaaaagaa gcttaaacac aaaatttgcc
    61681 gaaacattcg gacctaaaaa aactacacaa tacttctcac caggacgcat caatttgatt
    61741 ggtgaacaca cagattacaa tggaggccat gtcttccccg cttccatcac ttatggaacg
    61801 caaggtgtag cagcacctcg agacgataat aaaatattag tttattccac aaactttgac
    61861 gatatcggtg tcattagttt tactttagat gagctagaat atgataaaaa agcaaactgg
    61921 gcaaattacg taaaaggaat ggtccttaaa ttaaaagaag ctggacactc aatcgatcat
    61981 gggtttgagt tattagtgga aggaacaatc cctaatggtg caggactttc cagtagcgct
    62041 tcattagaat tattagtagg tgttgtatta gaagatctgt ttgatttaaa aacagatcgc
    62101 ttggaactag tccaaacagg taaaaaagta gaaaatgaat tcatcggtgt caattcaggt
    62161 atcatggatc agtttgccat cggcttcggt gagctagatc atgcaatctt attagataca
    62221 aatacgctga aatacgaaat ggttccagtg aaactagatg gttatgcaat cgtcatcatg
    62281 aatacgaata agcggcggga attggcagat tcaaaataca atgaacgccg tagcgaatgc
    62341 gaagaagcct tggcacgttt gcaaaaaact ttgcctatct cttctttagg agacttagat
    62401 gaagaagaat ttgaatcaac tattgaccaa atcggcgatg atacattgat acgcagagct
    62461 cgccatgccg tttctgaaaa tcagcggact ttgaaggcaa aagctgaatt agaagctggg
    62521 aacttagaag catttggtca actgttaaat gattctcacc actctcttcg ctatgattat
    62581 gaagtaactg ggatcgaatt agatacacta gtagatgctg ctcaaaaaca agaaggtgtc
    62641 ttaggtgcac gaatgacagg agctggattt ggtggctgtg cgattgctct tgtcaaagaa
    62701 gaaaatatac ctgccttcaa aaacaatgtc tatgatgaat acatgaaagt tgtcggctat
    62761 gctccggaat tttatgttgc acacattgga aatgggacaa caaagatcga tccagaagct
    62821 gttcatgttt gataaaaaaa tcaacgatct cagctaaaaa caaatagata aaaataacct
    62881 ctcagcaatt tccaaatgtg agccttttgc tcgtatttga aaattgctgt tttttattga
    62941 cacggaaaga gaacgtgtgt tctaattgtg ttgaagaggt gattgttatg tgggcgaatc
    63001 aaaatccggt atttgtttat gaaaaagaac cttcaagaga tattttatgt attgactgca
    63061 aatcttttta cgcctccgta gagtgcgttg aaaaggatct tgatcccctt acaactaaat
    63121 tagtagtcat gtcctatccg tccgattcga cagaaacacg gggtagcgga ttaatcttgg
    63181 cttccagtcc tgcagcaaaa aaagcttacg gcatcaccaa tatcagccgt gccagagatc
    63241 ttcccttccc ctatccaagt gatttatata ttgttgcccc aaggatggcc tattatatgg
    63301 aaaagaacag agaaatcaat tctatctata aaaaatatgc agacgaagcg aaccatcatg
    63361 tctattccgt cgatgaaagc ttccttgatg taacaaatag catggcactc tttggtaaaa
    63421 caaatgcgta cgacatggct aaactgatcc ataaagatgt gtttgagcag accggaatct
    63481 acacgactat aggaattggc gataacccgc tacttgctaa actagcctta gacaatggct
    63541 caaaagacgg aaagcaaatg tttgctgaat ggcgttatga agatgtacca gaaaagttat
    63601 ggaaaatctc agatatcact gacttttggg gaatcggcaa acgaacagca aaacgtctga
    63661 aacaaatggg tatacattct gtttatgatt tagctcacgc ggattattat cacttgaaaa
    63721 gcaagctagg ggtcatcggt actcagcttt atgcccatgc ttggggcatc gatcgaagtt
    63781 ttttgggaca agaatataca ccaaaatcaa aaagtatcgg aaatagtcaa atcctagata
    63841 gagattatac aagacgagaa gaaatcgaga tcgttatcaa agaaatggcc gaccaagtcg
    63901 gcactcgctt gagacgatct gcagcaaaaa cacaagtagt cagcttatgg gtaggatact
    63961 ctttaggcta tcttgatcca aatggcaaga ccgggtttca ccagcaaatg aaaatcgaag
    64021 cgacaaatgc tagcagagat ctagcagatg ctttattgac cattttccac cagcattacc
    64081 actaccagga tattcggcgt gtaggcgtga actgttccga cctcgtatac gcgacaggtt
    64141 tacagcttaa cctttttgaa gatcctgaaa agcagataaa tgaagcgaaa gttgattttg
    64201 ttgtcgatac gattagaaaa aaatacggct ttcaatcaat catccatgcg catagtctct
    64261 tgcaaggagg acgcgccatc gcacgtagtt ctctcgttgg cgggcatgca ggtggtatga
    64321 gtggattaga aggtggaaaa gatgagacgg acagcaaaaa aattcattga ctataacgag
    64381 tatcgagatc ggccatttgg attaaaatgg gggactgctt ttgcgatgga agaactagtc
    64441 aacggtatca aaaaaatgaa gaagaagcac gcaaacagcc tcttcctaaa caacaaatga
    64501 cattgcaaga aatcgatagt atactgacag aagccttttt atctcgtaaa gcagtaatca
    64561 ttcagctcaa tttaaaagat gaatttggaa gactcttgga tgaggtcgaa gggcacttca
    64621 ttggtgaagc ctatgaagat tactttatga ttgaccatca gccgatattg tgggaagacg
    64681 tacggtatat tgagttaaag gaaacaaaaa aatggtttgt gacatgagag gttgtgacag
    64741 aagtgatcaa cttcaaaaaa taagaaagga tttttcgaaa attgcttctt aaatttttgt
    64801 ggaattttgg cttatttttc gaagaagcaa cttctggaac aacgtatatt agatatcaga
    64861 ggttgtgaaa aagtcgctta gttttgagta acaagccaag gcgctgattt cacaacctct
    64921 gtctatctat ccatctattc ggtaagattc atgcatgacg tcttctttat ttagtatgta
    64981 gagccctttt tcttctgacc attcaacata aatcagctgt tttacatctg catagccttc
    65041 tttcttcaag ttctccataa gccatgcttc agtcaattga ttggactgaa gcactttatg
    65101 ctgaatttgc ccttcatcta caagcaggat cgataaagct ttgtcttcat ctgatttttt
    65161 caatacactc atttgaccgg cattctctag gataacatgt ttggcatttt caagaaaaaa
    65221 gcattcttgc tgtcttaaga ttgctcgcaa ctgctccatc tcgatatggt tatttgtcaa
    65281 ctctgtcatg ttgatgacgc cgtctttgat tagaacagat ggctcgcctt tgacccaacg
    65341 attgaccttt tcattttttt ggacgatacg ttcgatacca taaatcatta ctgcccaaag
    65401 cgcaatggca aataggacat gccctacatg gacattgtca tcataggttg attcttctaa
    65461 gatcccacct aaaacaagcg tatagactaa atcaaacggt gtgatttctg acatggattt
    65521 cttcccaagc aaacgtacca ctaataataa accgatcaac ccgaacacca gtttcattgt
    65581 aattgaaaaa atcatttttt ctccccctaa ctatctgtta attaaattat atatgctttc
    65641 tcacgaacac gcatggaata tacaaggaag aaatgtatcc ttttactaaa aaaaggactg
    65701 tgacactgta gtgaccccca aaagttagac ttttttggag tggagaaatc cactcctttt
    65761 ttttatgctc taaaatgata tttttacaca accgttacgg ttgttttacg aaattggacg
//
LOCUS       DASHRQ010000008        64551 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.008, whole genome shotgun sequence.
ACCESSION   DASHRQ010000008 DASHRQ010000000
VERSION     DASHRQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.008"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(21..251)
                     /locus_tag="U2M88_000551"
     CDS             complement(21..251)
                     /locus_tag="U2M88_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372957.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="U2M88_000552"
     CDS             complement(687..1253)
                     /locus_tag="U2M88_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEM8372958.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="U2M88_000553"
     CDS             complement(1253..2209)
                     /locus_tag="U2M88_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEM8372959.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="U2M88_000554"
     CDS             complement(2209..2865)
                     /locus_tag="U2M88_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEM8372960.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="U2M88_000555"
     CDS             3235..5253
                     /locus_tag="U2M88_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEM8372961.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5323..5529
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5680..8094
                     /locus_tag="U2M88_000556"
     CDS             5680..8094
                     /locus_tag="U2M88_000556"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEM8372962.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="U2M88_000557"
     CDS             8442..9995
                     /locus_tag="U2M88_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEM8372963.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="U2M88_000558"
     CDS             complement(10376..12160)
                     /locus_tag="U2M88_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8372964.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="U2M88_000559"
     CDS             complement(12175..13896)
                     /locus_tag="U2M88_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8372965.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="U2M88_000560"
     CDS             13996..14757
                     /locus_tag="U2M88_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEM8372966.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQFLLTNTDENNIRTEYYHAWIEMKK"
     gene            15724..15992
                     /locus_tag="U2M88_000561"
                     /pseudo
     CDS             15724..15992
                     /locus_tag="U2M88_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16664..19786
                     /locus_tag="U2M88_000562"
     CDS             16664..19786
                     /locus_tag="U2M88_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEM8372967.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKA
                     NRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVY
                     QLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQG
                     AVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIE
                     KEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFI
                     SIKFRKKRNI"
     gene            complement(20210..20380)
                     /locus_tag="U2M88_000563"
     CDS             complement(20210..20380)
                     /locus_tag="U2M88_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM8372968.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(20574..20753)
                     /locus_tag="U2M88_000564"
     CDS             complement(20574..20753)
                     /locus_tag="U2M88_000564"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372969.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            21140..22252
                     /locus_tag="U2M88_000565"
     CDS             21140..22252
                     /locus_tag="U2M88_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEM8372970.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(22330..22623)
                     /locus_tag="U2M88_000566"
     CDS             complement(22330..22623)
                     /locus_tag="U2M88_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEM8372971.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            22791..23105
                     /locus_tag="U2M88_000567"
     CDS             22791..23105
                     /locus_tag="U2M88_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEM8372972.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            23126..25105
                     /locus_tag="U2M88_000568"
     CDS             23126..25105
                     /locus_tag="U2M88_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEM8372973.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            25118..25444
                     /locus_tag="U2M88_000569"
     CDS             25118..25444
                     /locus_tag="U2M88_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEM8372974.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            25460..25978
                     /locus_tag="U2M88_000570"
     CDS             25460..25978
                     /locus_tag="U2M88_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8372975.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            25978..27330
                     /gene="celB"
                     /locus_tag="U2M88_000571"
     CDS             25978..27330
                     /gene="celB"
                     /locus_tag="U2M88_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEM8372976.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNSSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(27414..28919)
                     /locus_tag="U2M88_000572"
     CDS             complement(27414..28919)
                     /locus_tag="U2M88_000572"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEM8372977.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(29011..29601)
                     /locus_tag="U2M88_000573"
     CDS             complement(29011..29601)
                     /locus_tag="U2M88_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEM8372978.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            29780..31426
                     /locus_tag="U2M88_000574"
     CDS             29780..31426
                     /locus_tag="U2M88_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEM8372979.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            31475..32194
                     /locus_tag="U2M88_000575"
     CDS             31475..32194
                     /locus_tag="U2M88_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEM8372980.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            32673..33620
                     /locus_tag="U2M88_000576"
     CDS             32673..33620
                     /locus_tag="U2M88_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8372981.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            33632..34552
                     /locus_tag="U2M88_000577"
     CDS             33632..34552
                     /locus_tag="U2M88_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM8372982.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVN
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            34593..36050
                     /locus_tag="U2M88_000578"
     CDS             34593..36050
                     /locus_tag="U2M88_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM8372983.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            36395..37009
                     /locus_tag="U2M88_000579"
     CDS             36395..37009
                     /locus_tag="U2M88_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEM8372984.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            37002..38726
                     /locus_tag="U2M88_000580"
     CDS             37002..38726
                     /locus_tag="U2M88_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEM8372985.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            38723..40174
                     /locus_tag="U2M88_000581"
     CDS             38723..40174
                     /locus_tag="U2M88_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8372986.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(40647..42800)
                     /locus_tag="U2M88_000582"
     CDS             complement(40647..42800)
                     /locus_tag="U2M88_000582"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEM8372987.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(42802..43848)
                     /locus_tag="U2M88_000583"
     CDS             complement(42802..43848)
                     /locus_tag="U2M88_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8372988.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            43998..45287
                     /locus_tag="U2M88_000584"
     CDS             43998..45287
                     /locus_tag="U2M88_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEM8372989.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            45300..48002
                     /locus_tag="U2M88_000585"
     CDS             45300..48002
                     /locus_tag="U2M88_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEM8372990.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            47987..48844
                     /locus_tag="U2M88_000586"
     CDS             47987..48844
                     /locus_tag="U2M88_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM8372991.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(48915..49814)
                     /locus_tag="U2M88_000587"
     CDS             complement(48915..49814)
                     /locus_tag="U2M88_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEM8372992.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            49982..52153
                     /locus_tag="U2M88_000588"
     CDS             49982..52153
                     /locus_tag="U2M88_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEM8372993.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            52221..52562
                     /locus_tag="U2M88_000589"
     CDS             52221..52562
                     /locus_tag="U2M88_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEM8372994.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(52838..54592)
                     /locus_tag="U2M88_000590"
     CDS             complement(52838..54592)
                     /locus_tag="U2M88_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8372995.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(54603..55439)
                     /locus_tag="U2M88_000591"
     CDS             complement(54603..55439)
                     /locus_tag="U2M88_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEM8372996.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(55441..56124)
                     /locus_tag="U2M88_000592"
     CDS             complement(55441..56124)
                     /locus_tag="U2M88_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEM8372997.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            complement(56283..57677)
                     /gene="uxaC"
                     /locus_tag="U2M88_000593"
     CDS             complement(56283..57677)
                     /gene="uxaC"
                     /locus_tag="U2M88_000593"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEM8372998.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            complement(57716..59215)
                     /locus_tag="U2M88_000594"
     CDS             complement(57716..59215)
                     /locus_tag="U2M88_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEM8372999.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            complement(59208..60629)
                     /locus_tag="U2M88_000595"
     CDS             complement(59208..60629)
                     /locus_tag="U2M88_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEM8373000.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            complement(60689..61681)
                     /locus_tag="U2M88_000596"
     CDS             complement(60689..61681)
                     /locus_tag="U2M88_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8373001.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            61913..64111
                     /locus_tag="U2M88_000597"
     CDS             61913..64111
                     /locus_tag="U2M88_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8373002.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
BASE COUNT        20796 a        11488 c        13019 g        19248 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtttctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata agtatttaga gatttttctt ctaacattct tcaaacaaga
    15301 aaaatcacaa aggttattgt tatttttata tattcttttt aagaatgatt gtttttttat
    15361 agaaatttta aaaaataaaa cacgttattg atggatagtt agtttgttat ttggtaatat
    15421 tatagaagtt gactggtagt gatttatttt tcgtcgtttt ataaaaatgt tattttttgg
    15481 aaaaattcac aaatatgtaa cgaatgtttt tctggaattt gctgtgttaa aaagcagtat
    15541 gactaatata aatactattg tcatttatgc aattttttat ggatagtaat tttagagggg
    15601 aatcaatgaa ggaagaatag aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata
    15661 gcaatacatc ttcaagcttt aaaattcgag agaatcatct tggtagtgga atcaactaca
    15721 acaaaaggat taaaccaatt aatagataga attaccaatg gaaaaataga aaaggtagtt
    15781 attctataca aaagttgttt aattcgcttc ggttatgaac ttagtgaaac cctttgcaat
    15841 atatggtaca gaatatagaa attattgaca ttacagaaaa tgatgaagat ttaatacaga
    15901 ttgtcactat ttgtagatac cttttacaag gaaaccgagc caataaagct aagaagatga
    15961 ttaaggagtt gatggaagac gatacaagtt aagaaagtga aactacgccc aaataaagag
    16021 caaaaagagt aattggacgt ggaaaatgca agaaatgaac catcgattcg gtggacagtt
    16081 tattagtaat gacggtttga gaacatccat gacgagaatg aaggaacatc ctaaatgcgt
    16141 ttagttgaac caagtgccaa ataatatggc caaataagcg atcagttatc gatgaatccc
    16201 aagtattcta aactatatat tcattttgat gctaagcatt gatatatttt tttgggattg
    16261 atgaaggtta accaatacag ggactcactg atgaaattct agagtctgac cttggtatca
    16321 acgatttagt gattgtttct aatggaaaag tataaaagta tataagtaca ttaacaaaat
    16381 aactattagg gttacgctcc agcttgtgca aggcactggc aggaggaatc gtgaaatcat
    16441 aattatggtt aagccacacg gacaactaaa agtcatacaa agctatcgaa tcaatcaaca
    16501 gaagacaatc ggcaatcatt agctttgttt acagcttaca ttgcaacgct tgagttgaac
    16561 cgaaaatacg agggaggtaa cgcagccatt ttgagttggg gaatcttttt agaaaattat
    16621 cgggtacctt gttgtttagt caaaattata agggggagta atagtgagga acaaatttca
    16681 taaactgtta agtatgtttc tagttatgct tattgtgatc aatcaatttg ggaacataac
    16741 ggttctagct gagtctttag cagaacagcc agagcgttcg ctgacgatcg aagttgatca
    16801 agaggaggct gctttgggtg agcagttcca gttgcgagtc agaggaacac cagacctgct
    16861 aaatgcattg gtcattgata agagtgagga tttgattgaa ggggaagtga agcaactcaa
    16921 tgattacgaa cgtctgatta cttattcttc ccagcattca ggtaaatacg aattgaccgc
    16981 aagaagcggt gatattcaat caaatacggt ctcaatagaa atcagtgtac cagaagaaag
    17041 cacaagtgag tcttttaatg taccagaaga aagcacaagt gaatctttta atataccaga
    17101 agaaagctta agtgaatctt ttaatgaagc cagatcatct gagccatcta taacgcagga
    17161 atctcagatt gaaagacaag aggagctctt gaattatgac atcggcatct ctgctgagcg
    17221 ccccactttg ccgagcaggc atacacaaac gttaaaggga tacattcaag gaggcgctgg
    17281 cgatattctt gaagaatatg gggagatggt cgtacgagtt cctagagcat cgattggctt
    17341 aattgagttc attagagtgg aggtcgctga tgattcacct ttaaagccac tggcatttta
    17401 ccaagaaggc acagacgttg tttttccatt tacctttgat tatgaaggat taaatgaagc
    17461 cgctcagtcg tatacattca gtatcacgtt tgaagttgct ttctttcaat caggcggtac
    17521 tgagccaaat aatgactttc aagtgagtct tacttatgct gagcgagcag caaacacgga
    17581 attttcaatt acgcctattc cttcatcaag acccattttt tcgaaattat actatggctc
    17641 tagagaaatc gacagccaag gaaatcttgg gaatctgctg attgacgggc aagacgtgac
    17701 caacagtaat aaatttattt tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt
    17761 ggttaccgat gtactaccgc aaggaacaac tttggcaccg aatccgacta catggagttc
    17821 cactggaaat agagaaccta tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg
    17881 tgaagcaaac gttagaactc attatgtttc cgaggaattt gtggacaaag tatcttttaa
    17941 taaggaaacg aatacacttg ttgtggactt tggtaatcta gcagagggtc aatcgtattt
    18001 tattgaatat gctcttgaag tgaccgatgt cgacttgttc aatgaaggaa gacaagagga
    18061 tgggttttct cggaatcgag cgagtatgac ctacaataat caggtaatga gccgagacta
    18121 ccgcttgaga atccctgaaa gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa
    18181 aaatgttatc aatctcaatg aaaatgaact aacgtatact ctgcatgtct cgatgttttc
    18241 tggaacaaga aatacgcttg ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc
    18301 atttctatca gatattccct ctttcattga tgccgaagtc aataatggaa tagttcgttt
    18361 cagtgcgaat aatacgattg gtggtccgac aagttttacc ctctctttta aggtcaatgt
    18421 agaggcgtat gcggtcggcg atcaaatttt aaactttgca acgcttgtga atagtgacaa
    18481 tcgattgaat acgagtactg taagaacaag gaaaatcgat gggcgtatta aagtaattaa
    18541 aactgatgag aatggccaaa ggctggcagg agcaaaattt gaaattttat caagcgattc
    18601 acaagtgata caagaaggta taacggcaga aaacggagaa tttttaagtg aaccgcttcc
    18661 tgtaggtagc tatcaagtcc gagaaacaga agcaccggaa gggtatcttt tagatgcgac
    18721 ggtacatgat gtttttataa atgaatactc catagaagaa gtgacgttaa ccattgaaaa
    18781 catccttgat acgggtggtg ttgaactaat taaacatgct gaaggcagtg gtgaagtcct
    18841 tcaaggagct gtttttgagt tacagaaccg agaaggagaa acgctacaaa ccggtctaac
    18901 tacaggagaa gatggaaaac tggcaattga cggtttagca ccaggagcat atcagctggt
    18961 agaaactcaa gcgccaacgg ggtatgaatt agatgcgaca ccgatcgagt ttgagatcga
    19021 aagaagtcag acagcggtgg tagaattgac gaaagcaaac cgacttacac caggcggtgt
    19081 cgtgttgaca aagatcgatg accaaagcgg ggaagtcctg caaggagctg tttttgagtt
    19141 acaagatgcc aatggaaccg ttctgcagtc aggtctaacc acaggagcag atggaaaact
    19201 ggcgattgac ggcttagcac caggagtgta tcagctggta gaaacccaag cgccaacggg
    19261 gtatgaattg gatgcgacac caatcgagtt tgagatcgaa agaagtcaga cagcggtggt
    19321 agaattgacg aaagaaaatc gacttacacc aggcggtgtc gtgttgacaa agatcgatga
    19381 ccaaagcgga gaagtcctgc aaggagctgt ttttgagtta caagatgcca atggaaccgt
    19441 tctgcagtca ggtctaacca caggagcaga tggaaaactg gcgatagatg gcttagcacc
    19501 aggagcatat cagctggtag aaactcaagc gccaacgggg tatgaattgg atgcgacacc
    19561 agtaactttc aagatcgaaa aagaacaaga agtggcgatc tttttaacaa aagagaatag
    19621 aaaagttgct gatagctcag atataagagg aaacaatcca acaagcagtg gaaataagca
    19681 tcttccaaaa actggagaaa ccatcattag ccagtttata ttgtcaatat tgggtgtgct
    19741 acttgttttc atcagtataa aatttagaaa gaaacgaaat atatgagcta aaataaaaaa
    19801 ttgaataagc atagttttta aaatcaacta gtacccatca attgaatgga aactaccgat
    19861 tttagaaggg gtatacaaac aaatattagt atcggagtag agaaggttga acccaaccag
    19921 taaataagta aagacaattt tgtgctgcgt tgagaagtga aaaataaacc tttgtagtaa
    19981 tactagttaa taaaaaatat acaaaaacac tgcgaaaatc attcttgagt ttcgcagtgt
    20041 tttttgccta atgactcaag tttattgggt aatactttga ttttctttct acaaataatc
    20101 agcgatcagt tgtaattttt tctctagttt taatggctct ttgtcaataa ggagaaaaac
    20161 aagtcgggtc ttttgtctcg gaaagcttca accaactcgt gaataaaggt tacgcctcct
    20221 tgaaaggcgt ggaaaaacca agtaatcgat caatcaccaa tttcgcattt ggtagaacat
    20281 gtttggtgag ctgaaagtag gtggcgttca tgtctgtaac taagaattct atttctttag
    20341 gattagtgca gctaaggaaa tagtttgtta atcgaggaat tttttgtgta ggtaaatttt
    20401 ctcgattaat ttttctgtct cgccatccgc ggaaataaaa ctcagtttat cctcaacgga
    20461 aacgtgagaa cgaaattcac aaccctcaac actcaagaca gaattctttt ggaagacttt
    20521 ggtaaatagc ttctcaactc tttcaatgtg cgaataacgg ttgtcaaaga tacctaacaa
    20581 cttttcgaaa taaaaaataa cgacactttt tcagtcagta aagaagtgat tttatgtctg
    20641 acatgatttg aaatcgagtg ccttggacgg ataaaataac tttgagcgtt ccaatggctt
    20701 cgacagtttt tacaggtata gcgctgcttt tttaggcgta taaccattgg catatgatta
    20761 tattattcaa agtggacaat ggtttccttc ttcttgtttt tcaccaccat taatttccta
    20821 tttccatcta ccacagcaga accacaattt ctacagacac atggagcagg gaagagaact
    20881 gcagcaacga tcaatgtttt tttcttctgt aaggtttcgt aagagacctc tgtaatcatc
    20941 aaatttttgt ctattgatct cagaattttt ttgatagaac cattcataaa gcatatcgtt
    21001 ctctcagttg tttattttgt ggtgatttaa tcatacaaga aaacgatatg tttttttata
    21061 aaaacatgaa taggaaaaat agttgacaag aaaatgacaa cgccatatga tgttccatag
    21121 tatttacaac ctgtcataga tgactgtaaa aaataaaagt aaaaagaaag gacgacaaca
    21181 ggtggatttt tatcagtctc tacaactaga tccgtttatt ttaaaacaaa aaatacgaga
    21241 agcagcttct aaaaaagaaa agtgcatgta tatttgttca ttgtttttac gatcattatt
    21301 catcgtattg tttgccattt gttttatcat tataattaca acattatttg aatcgaagca
    21361 taaaccatac gctgtggttt tattctgtat gttgatgagt attcgttttg ttgactttgg
    21421 ttataagatt tcccattcga tcataggttt agcaatcgtc atgttctcac tattagtagc
    21481 accgtatgtc cagctgatca aatggtcagt tatgggcatg ttgattcatt ttattttact
    21541 aagcagcata ttaatggcaa ccgctagtga tcccaaaatg gggaatgctt ctttgtacgg
    21601 cttttcatat ctctttattg tttattcgtt acctaaagat tcgttgaaca agaatttctt
    21661 tacacaaaca agtagcttat tatttttgtt tttttgttgg ttttcagttc tcctttacag
    21721 aaaacatcga gaaaagaaca aagacaaaag cttgtttaaa gagatttttt tgaaaggcat
    21781 gtattcgcag gaaaaaattt ggatgctcat ctatgcattt ggcattagtc ttttgatcgt
    21841 tgcaggagag tatgtccctt ttcagcgatt gatgtgggca gggtttgcat tttcttctat
    21901 tgtatctagc tatggattga tgtcgattgg tttcaaagaa cgagcagtag accgaatcat
    21961 tggctcattg attggatgtg ttttatttat tggtatcagc caatttattc cttttaactg
    22021 ggtcggtata ttaggtggtc tagctttagg tgtttgttct acctatagat ataaaacagt
    22081 ttttaattct tttggtgctt tggcaatcac tgccagttta ttcggggttc caggagcagt
    22141 aacgatccgc atttttgaga atatccttgg ggtatgttta ggaatcatgt atattggggt
    22201 gactgaaata cttatccgaa aaataagaga aaaacacggt ttgaatcatt agatgagatc
    22261 gaataaaaaa taaggaaaaa gtgaagatac acatggatga taaattgcta tcattccatg
    22321 tgtatctttt tacggtaaat cagtgaaacg ttcgactttc atttttaatt tatacttttt
    22381 acggagttcg tttatttgag ccatcatttc ggatttgtga tgcttttcga ttgcttcttc
    22441 atctttccaa cggtcgatca gtaaaagaga ttctggatct tctagtggaa agtaatagtc
    22501 atatttctcg tttccttctt ctgcacgaat cgctttgaca atgtttcttt cgaccatttc
    22561 ttctgcaaat tttctggctt gtccatttgt acttgtatac aaaatattca ctgtgatact
    22621 cactaaatcc cctcctgaaa aaattctatc actagtatat cacggtgcca gaaattatta
    22681 ttcaagatat gatacacatt ttttccgcaa tgctacggaa atatatattt tgaatagaaa
    22741 acgcttacaa gatatgcttg gtttataaaa ataaaaggag tgattgattt atgaaaaaag
    22801 cattgattat ttgtgcggca ggaatgtcat cctcgatgat ggcctcaaag acaacggatt
    22861 tctttaagca aaaaagagaa gaggtactcg tagatgcagt ttcagctaca gaaggcgata
    22921 atatgattaa gaatagtgat tttgatttat ttctgatcag cccgcaaacg acgatgtttt
    22981 tggataaatt caaaaaatta ggagaatctg ttggaaagcc tgttgttagt atccctttcc
    23041 aagcgtatgt cccaatccct acaggcatcc agaagttagc agaggtgatc gaagagaaca
    23101 tatgatggaa taggagtgga tgataatgaa acagaaggaa aaattgctgt tgagatattt
    23161 gattgatcac aagaaggagt atgtcaccag tcaacgttta gcttctgaat tgttattatc
    23221 tgatcggaca atccgtaatt acttgcagcg gatcaaagag ttagtagaaa aaaacggagg
    23281 taagatcatt gctaaaccag gttatggcta ccaactgcat attttacaaa gactgacatt
    23341 tgacttgttt ttgagtcggc aagaaatagt acctggatat gctagagaac cgcaaggatt
    23401 ttatgagtcc gaagatcgac aaaagtatat attgaaccaa ttgcttttgg aagatgccat
    23461 tctttttatg gatgatttgg cagaagagct ttatatcagt cgttccagtt tgacaaagga
    23521 catgcaagaa atcaaggagc gattgattcc atattcacta aaaatcgttt ctaagcatgg
    23581 tcaagggaca tggatcgaag gggaagaaag aaatcgacgg cattttatca tggatacttt
    23641 ttttggaaac cattatggaa attcattgaa agaatattta gggcaaagcc gttttttccc
    23701 agacatcagt tttgaagagc tagtcattat cattctcgat gaaacaagag aagctaagtt
    23761 aaaagtttcg gattttatca tacagaatct gactttacac ttggctttgg ggatcaaacg
    23821 gctccgagaa gggtttgaaa tcaaggaatt aggcatcgaa caagaagttt ctaagcgagt
    23881 agagtatcaa gtggcgcagc ggatcgttca acgtattgag gtaatcgcag gtgtatcgtt
    23941 tcccgaagaa gaaattgctt atttgacatt acatctcatg gctaagtcga atcacaaaga
    24001 aaatcaaaat gatcaggaac tatcaaagga attagcgaaa gttctgaaaa gattgtctca
    24061 ggaatttcat ttttcattag tagaggatct ccagcttttc aatggactac tcgatcattt
    24121 gtcaccgatg atgattcgat tgagccgagg tattcgcatt gagaacccat tgactgagga
    24181 gataaagaaa gaaaatccca aagtctttga cgcagtaaaa aggcatttca gcaacatgcc
    24241 ggctttgaaa aactatacca tcaatgaaga tgaatgggcc tatttggctt tgcatctcat
    24301 ggcggcattg gaaaaagaaa gagctgcgca taaactacat gcattgatta tctgtgcaac
    24361 gggttacggc agtgcccaac tattaaagaa tcgcgtagtc agtgaattcg gtaaaaatat
    24421 tacagtggta agtgtaaaag gctattatga gatcaatgaa acaactttaa aaggaatcga
    24481 cctgattatt tcttctattg atctttcgac aatgtttttc aaaattccag ttttacatgt
    24541 cagtattttt ttgaatgaag atgatgttcg taaaatacga aaggtagttg gagaatctat
    24601 accctatcat tcctcagata gaccgatacc agcattttct ctacagcaaa aaaagcagat
    24661 ttacaccgaa cagttgtctg aaaaattttt caagacttat acgtacgaac cgaaaaagga
    24721 agaaattctt catgatttgc tgcaaaagct gagcataaat gaagaagaag gatatataag
    24781 agaaatgcga aagcagatcc agcaacgtga aaatatggga caaatcatat tcagtgatac
    24841 agtggcagtc ccacatcccg ctgttccagt tggaatcgaa acaaaggtag ccgttgcatt
    24901 gattccttct gggatgcagt gggatcaata tcaagaaatt cattttgttt ttttagtttc
    24961 gccttcttat gtcgaaaatg aaggaattac ggtcgtgact aaagcaatcg ttaggttagt
    25021 tgaccagcta aaagtacagc aggaaatctt agctgaacct atttttgaga atttcagcag
    25081 taaatttatc aaactgatcg aataaggagg aaaacagatg gctgaaaaaa taaacagtga
    25141 aacactacaa gtgacttctt ttgatatcat tttccatagc ggaaatgcac ggacactgat
    25201 acatgaagca tttcgctcga tgcgtaaaga gatgtttgaa gaagtagaag agaagttgcg
    25261 acaagcaaat gaagagattc ttcatgcgca tcagtcacaa actcaacttc tgaaagaata
    25321 tgccagcggg acaaaaatag aaatggaaat tatcatggtc catgcacaag atcatctgat
    25381 gacgacgatg acattattgg aagttgcaca ggaaatgaat tatttgtatc aaaaaaataa
    25441 atagaaagga aggatcattg tgcaggatac agtaaaaagg aaaatcgaat tagaaaaaaa
    25501 gcaacttagt ttgaaaaaca tgtatttcaa tcgttatcta atcgtacgtt acctgactgc
    25561 ctttttcttt ttcatgaaca tgcaatggat gattctgctt ctatcagcta aaagtttagg
    25621 aagtagttta ccgatgattc tgattttagc tattttgcct gcagtaggag aacaagtaaa
    25681 gctttatcga aaacaccaaa cgaacgttcc atggacaaag tggtattttt tgttccagag
    25741 tgtctttaat ttattagtga ttccagtgtt gtttacctca ggatttacat tcttgtatcc
    25801 ttttatggca aataacaatg gaggacagtt atttgttttc attcttgttg taagcggtat
    25861 ttttgccagt gcatttatac agtatcgttt aaagaaaatt tcactcaacc aagaccaaca
    25921 gtatatccgt attaaacaat atgaaaaagc gttatattta ggaaaggaga ataactaatg
    25981 tttgattttt tgcagaaata cctgatggga cctatgggga aaattgcgca acttaaaatc
    26041 gttcgggctg tcatggcagc ggggatggct tctattcctt tcacgattgt aggctcgatg
    26101 tttttggttt taaatatttt gccattaccg ttcccatttt tagaaggatt ttttaatgca
    26161 acttttttca aagttagtga cttgtacatg atcgtcaata caatgacgat ggggatttta
    26221 tctatttact ttgccattgt gtttgcttat gaattaacgt caattgaacg agatgaacaa
    26281 aaattaaatg ttaatccgct cacgggggca ttactatctg tttttgcatt ctttatgtgt
    26341 atccctgaat tgatcatgac tgatggtaaa atcagcttga ttgcttctat caatgagaca
    26401 gaaactgtag taaatggtat taggatgggc tcttttgtcg aacgtttagg aacttcagga
    26461 atctttacag ctattattat gtcgattatt gcggtcgaat tgtattgtat gtgtgtcaag
    26521 agaaattggg tcataaaaat gccagatgtt gttccgccag gagtttctcg ttctttcact
    26581 gctctgatcc caacatttgt cattgcgttt gtggtgatga tcatcaatgg attattagtt
    26641 gcactaggaa cggatatctt caaaatgatt gctatcccat ttgggtttgt tacgcagttg
    26701 acgaatacat ggcttggtgt catggtgatt tatttcttga tccatgcatt gtggatcgta
    26761 gggatccatg gagcgaatat catcacttct ttcttgaccc cgatcgtatt agctaatatg
    26821 gcagctaacg cacaaggcgc aaattatccg ttggcaggag aattcaacag ttcttatgta
    26881 accgttggtg gatcaggtgc gactttagga ttgatcatct ttattgcatt cctcgcgaaa
    26941 tctgatcagc tgaaagtgtt aggaaaagcc tcacttgtac caggattatt caatatcaat
    27001 gaaccaatca ttttcgggat gccaatcgtc tacaatccct atttagcggt tccgtttttc
    27061 ttagctccga tggcttctgc tacactcgct tattttgcca ttcggctgga aatcgtcaaa
    27121 ccgatgcttg cacagatgcc gtggccttca ccagtcggga tcggagcatt tgttggtagt
    27181 ggtggtgatt ggaaagctgc tgtgttagca gtcatttgtg caatcatcgc attcattatc
    27241 tggttcccat ttatcaaatt ctatgataat aaattattaa atgaagaaag agaaaaggca
    27301 gcagaattag ctagtgaaaa gttaaaatga tttctaagaa aaaaggaact gtgacaaaaa
    27361 atcacagtcc ctttttttct tagaaatggg aaaccactag tatataaata ccattattct
    27421 tcaatcaacc gcttcacgat tgcataatcc ccttcataag gttcatctac accattgatt
    27481 ttttgatagg gatccacgcc ttttcccgca ataacgactg aatcttgcgg agcgctttct
    27541 gctaaagcgc gtcgaatcgc ttcaggacga tccatttcat aatgaacgat caaatctgga
    27601 ttagtaattg cttcattgat ttcttccgcg atttttttcg gatcttcaaa tgctgggtca
    27661 tctgccgtca aaaaggcgat gtcagctgtt tctgatagca cttttccaaa atcatgtcga
    27721 cgggagattg ctttgtttcc tgggctgcct aatacaacaa ttacacggcc atctggatgt
    27781 tcatttttgg caaatttcag caatgttttc aagcttagat aattatgtgc atagtccaca
    27841 tagacatgcg ctccattgga ttggatcaat tgatccattc ggcctggtac acgagcttct
    27901 tttaatccgt cttgtgcatc ttgactggag gcacctgtta gtgctgcggc aatgatcgca
    27961 ctggctgcat tttcttggtt gaatcctcca gccagcaaaa tatcataagc agtatcgcag
    28021 atagctaagc gatcttcatg agaagataaa gtaaaaccgt gagtaccttt atctgaacgg
    28081 atcacttgat aattgctttc ttccgaacga ccataagtga tcgttggaat ctggaataga
    28141 tcggctgttt ccctaagtaa atcaaaatag tcactttcat gatttaaaac aactgttttt
    28201 gaattcaaga tcaattgtcg cttgcaatag aaatagtcat caaatgtcgg atgttcgatc
    28261 ggactgatat ggtcaggcga gatattcaag aagattccga catctaaagt caagccgtat
    28321 acgcgttgtg ttttgtaagc ttgtgaagaa acttccatca ctagatgagt cataccattt
    28381 tcaactgctt ctgacatcat acgatataaa tctaatgact caggcgttgt caaatgggat
    28441 ttgaaatatg ttttgccatc taacgttgta ttcatagtag aaagcaatgc tgttttttta
    28501 tttgttgtgt gatccaagat tgcttttgta aagtacgcag cagttgtttt accttttgtt
    28561 ccagtgaatc cgatcacttt caatttattt tgcggatagt cgtaaaaagc catagcaaga
    28621 agcgccattg ctttacggat atccgttacg atgattccta ctgctgaatt ttcgtatggt
    28681 tgttctgata cataatattg aataccctgt tcgattgctg aatccaaata ttcttctttg
    28741 aaattcattc ctttacagaa aaacaaagta gaagcatcag cttttcttga atcataactt
    28801 aactgaagaa attcagcttt ttctggtaca tcaagatgcc agcctcttgt tgaaacaaat
    28861 tcttttaata gattttcttt taatagtaaa tgacggatgt cttctaaaga gaagctcatt
    28921 gattgatccc tcgctttatt caatttcttg gtttattata gcacactctt tttaatatat
    28981 cgtttgtttc cgctatgaca aaatccagct ttattcaaat tttcttgaca caggggacaa
    29041 gcggatgagt attttcataa acaatgaact gactgtaata gataggagaa cgaatagtgt
    29101 ctccaacaat ccaaaaacat aataattcaa aaagacgata agactattgg taagtaaaag
    29161 tccttgcggc attggaagcc gaaaaaattt gtggaaaata atcggcggta tcgttatacc
    29221 gccatttgag gccccgactg tgtacaatgt gtaaacccca agactgaaca aaagacttcc
    29281 tgaaatcagt gaaaagaaat gtgaggagat catttccact tttggaacaa tctctaaaac
    29341 aacaggaaaa agcagacttc ccgctacagt cttaaaaaat actgattttc ctaataggac
    29401 agcggctaga agcagcatca taagattgat gctccaaaca gtaactgagc gttggataga
    29461 aaaggccgtt tcaactaaat gtccgatggc gcccactccg gcggaagcta tatcgtgagg
    29521 gagcaagaac caattcaatc caactgtgat aaagaaaaga cctaaaaata taacgggtag
    29581 tttttttatt tcttgtgtca ttgatatctc ctataaatgt tagccataaa aggctgtata
    29641 tttctttact ttaccaaaaa aacgaaaccg aattcaaaaa aacaattgtt cttataaaag
    29701 ttatataaaa tcgattgcca gtttctgttt taccaattaa aatgatagta atgttttgta
    29761 gaaagttgga atctgaccga tggataatca aagaaaacca gcattagatc aattaacacc
    29821 gcaagaaaaa atggcacgag gctctgcttg gatgaccgcc agtaatatca tttcaagatt
    29881 gctaggcgct gtctatatca ttccctggta tgcttggatg ggcgaaaatg ctaaggctgc
    29941 caatggatta ttcaatatgg gatacaatat atatgcattg tttctactga tctctacagc
    30001 aggaatacct gctgcgattg caaaacaaac tgcacgctat aattcattga atgaatatgg
    30061 cacttcacga cgattattta tccgtgcatt acagatgatg ggcgggttag gcctgttgtt
    30121 tgctttgttc atgtacatcg cctctccttg gctagctcat gcttctggtg gcggagaaga
    30181 actgattcca acgatgcgtt ctttgagtat cgctgtattg gtttttcctt gtatgagtgt
    30241 gatacgaggg tatttgcaag gcaatcaaga aatgatgcct tatgcgctgt cgcagattgt
    30301 agaacaggtg gcacgtattt tttatatgct attggctaca ttcatcatca tgaaagtcat
    30361 ggatggtgac tatgtgacgg cggtcacgca atcaactttc gcggcattta tcggtatgct
    30421 agcaagtatt cttgtccttt tgtatttttt gaagaaacat caagcgtata catcggcgtt
    30481 tattcactac agcgaaaaca aaataaatat tgcaacgaag gaattgttgt tagataccgt
    30541 taaagaagcg attccgttta ttattgtggg ttcaggggta acgatcttca aattagtcga
    30601 tcagttcact tttatgaaga tcatgtctgc atccaccgag tattccaatg cacagttact
    30661 ggatcttttt tcgattttta gtgccaatcc agataagttg acgatggtag ttattgcatt
    30721 ggcaacatcg atcgcgtcta ctgggttacc gttgattaca gaagctgtta cgttgaaaga
    30781 tcgagtaggt ctggcaaaat tgacaagcgg aaatttacag ttgttttcat tttttatgtt
    30841 tccggcgaca tttggtgtta tgttacttgc ctatccgcta aatacattat tttatacacc
    30901 ggattctcta ggaagtaatg tactgatcca agcaagtttt gtaggactct tcctaggact
    30961 atatatgcta gtttccaaca tgctgcaagg aatgtttgaa aacaaggcgg caattaatta
    31021 tttggttatt gggtttctag tgaaactcct tcttcaatac ccagccattc gtttatttga
    31081 agtatacggc cctttattgg cgacaatgat cggatttgct gtgtcttgta ccttgatctt
    31141 aaagagaatc catgaagtag cccgctttaa accaagtttt gtctggcgca ggacattgct
    31201 gatctttatt cttaccttga ttatgctttt ggcttcgtgg atcacaaaga tgatttttgg
    31261 tgctttttta aacgaagata gcaaattcca gtctctattg ttgattcttg tagtagcagg
    31321 tataggggct ttagtgtatg gttatttagc tttgaaaatc cgcttggcag aaagactact
    31381 aggaccagga atagcgcgtt tacgacataa attaaaaata aaataagtga atcagaagag
    31441 ctagattgat gctcttttga aatgggggaa acgcatgcgt ttagataagt ttttagcaga
    31501 tatgggactt ggcagccgca aagaagtcaa aggattaatc aaaaaaggaa cgatcgaagt
    31561 caatggacaa gtggtgaaaa gcgataaagc gcaagtaaat gaattcgaag atgcggttac
    31621 ttatttagga gaaatcatta cgtatcaaaa agatttttac tacattttgc ataaacctgc
    31681 aggagtcatt tctgctactc aggataatca tgatcaaacc gtaatcgatt tattggaaga
    31741 ccaggattat cgggaagatc tttttccagt cggccgatta gataaagaca cagagggcct
    31801 cttgatttta acgaatgacg gggtgtttgc ccatcagtta ctatcaccta aaaaacatgt
    31861 ggagaaagaa tatctagctg aggttagcgg tataatgaca gaagaagata tccgtttatt
    31921 tgcagaagga ctgattattg atggcgatga gcagacattg ccggcaaaac tccaaattga
    31981 tcaagtagat caagagacag aaacttcaat tatccgtctg attttgcatg aaggaaagtt
    32041 tcaccaagtt aagcgaatgg tcaaagcagt aggaaaagaa gtcttatatt taaaaagaat
    32101 ccgtatggga aaattttggc tgcctaaaga tttgaataaa gggcaatatc gacccatgac
    32161 caaagaagag ctagaacaag tgagagaaaa ataattgaat ggatagctaa aagaataagc
    32221 agagtagttg tagcgccatc tgaaaagcag acttgtccgt ctgactttcg gatggctttt
    32281 ttgtgaaatt tcggcttatt tccgtagaag ctacttctgg aacaccgttt gttcggaata
    32341 agggactgtg acaagacttt tgttacagtc cccattgttc cttgtgactc agagttagat
    32401 agtaaggaca cgatctccct aatactcagg ccaaaacgct gaattcacaa cctctgataa
    32461 acggtgttca atcagctgac cctcggtatt agctcaaaaa acggcaaaat atgaggaact
    32521 attttcccgt ttttttctct aatactcagg ccaaaacgct gatttcacaa cctctcctat
    32581 actttgtttc attttcccta tactttgttt atttattctc attattgaaa gcgctataat
    32641 tacgttgcga atgaatcgaa aggggttttt agatgaaaaa acgaaagggg ttcaatcatt
    32701 tttttatgaa tctatggcgt tataaagcac ttgtcctgat ggctgtaccg ggtatgcttt
    32761 ggatgatttt tttcttttac atacctgtaa tggcgaatgt cgtagcattc aaaaattttc
    32821 atatttcagc agatgggttc ttggctagct tgagggaaag tccttgggtg ggttgggaaa
    32881 atttcaaatt tttattttct tcggatcaag catttctcat cactaaaaat acaattttat
    32941 ataatgtagc atttatcctt ttgaatctgc tgatctcagt cgtatttgca atcattatga
    33001 gcgagttgag aaataaacga ctcgttaaag tttatcaaac gatgtcctta ctaccttatt
    33061 ttctatcttg ggtcattatc agttactttg tttacgcttt tttaagtcct gacaaaggaa
    33121 tattcaatca gtggatcact ggacatggag gagaagctgt caattggtat aatgaaccga
    33181 aatattggcc gtttatcttg gtgttcattg gtacatggaa agggattggt tataacagca
    33241 ttatttactt tgcatctgtt atgggaatcg atccgactta ttatgaagct gctatggttg
    33301 atggagctag caaatggcag cagatcaaac atgtgacgat accacaactt attccactaa
    33361 tgacgatttt aacgatttta gccgtaggaa acattttccg agctgatttt ggcctgtttt
    33421 ataacattcc taggaattcc ggtgcattat atgatgtgac tcaagttttg gatacgtata
    33481 tctacaatgg cctgacatcc acaggagatt ttggtatgac ggctgctgca ggactttacc
    33541 aatcagttgt cggctttgtc ttgttgatga tcacaaatac gatcgcccga cgttttgata
    33601 gcgattctgc attattctaa ggaggaaaaa aatgaaaaaa aagaaagtgg agagagtcca
    33661 agtccgctct ttcaataaga cggctaattt tttctctaat ataatgattg cactctttgc
    33721 tatttcctgt attttgccat ttatatttgt tatcgccatc tcttttaccg atgagtccat
    33781 attgtcgact cacgggtaca gtttttggcc agaaaagttc agtatatttg gttatacgta
    33841 cttgttcgac cagatgcagg ataaaatttt ccaagcgctt ttcgtcactg tacttgttac
    33901 tgttttaggt acactgatca atagcacggc tacttcttta tatgcttatg cgatttcacg
    33961 gtcaaacttt ccattccgtc gtttttttac cgttttttgt ttgattacga tgttgttctc
    34021 accagggatg gtggccaatt atcttgttat gacaaatctt cttcagttaa aagatacgat
    34081 ttgggcattg atcttgccaa tggctgtcaa tcccttcaat attattgtga tgcgcacatt
    34141 ctttaaacgt tcggtctctg attcgattat tgaatcggca cggatcgatg gagctagcga
    34201 actgcgaata tttgttcaga tcgttttacc gttagctgtt ccaggtattg cgacaatcag
    34261 tttatttgcc gcattgggtt actggaatga ctggttcaat gctttactat acatccaaga
    34321 tgataagcta gttccactac agtatctttt gatgaaaatc caaagtaata ttcaatatct
    34381 gacacaaaat tcaggagcag gaagccaatt gacaggcgga ttagcctcag ttccaggaga
    34441 atctgccaga atggcgatcg tagtcatctc gacgttgcct attgcgatca gctatccatt
    34501 tttccagaag cattttgtca aaggtctaac aatcggcgga gtaaaagaat aaagaaacaa
    34561 aaatataaat cataaaatta ggaggaagaa aaatgaagtt atggaagaaa ctagccttta
    34621 caggtgtttc agcgttgttg ttgggaacat tagcagcatg cggaggttct ggttctaaag
    34681 accaggcaga agctagcaat tcggacactt tgcagatgta tcaaatcgga gataaaccag
    34741 ataatttcga tcaattgatg gaagtagcaa acaaacgaat cgaaaaagag atcggtgtaa
    34801 aagtcaatat caattacatt ggctggggcg attacgaaaa gaaaatgaac gtcatcattt
    34861 cttccgggga aaattatgat atcgcctttg cgaacaacta tgtttccaat gcccaaaaag
    34921 gagcctttgc tgatttgact gaattagcgc ctaagtacgc tgaaaaagct tataatgatt
    34981 tagatgaagc atatattaaa ggaaatctgg tcaatggaaa attatatgct ttcccagtca
    35041 atggaaatgt ctttgctcaa caagtactta cttttaacaa agctttacta gataaatacg
    35101 atctttcaat tgatggaatc caatcttacg ctgatgcaga aaaagtcttg aaggaattcc
    35161 atgaaaaaga accaaacacg gcagcttttg ccattggaca aggcttcaaa gtccaaggga
    35221 actttgatta tcctttaggg aatacgttac catttgctat tgatctggat ggagatgatt
    35281 cgaaaatcat caaccaatac gataatgaaa cgtatatcgg actgttacgt acaatgcata
    35341 gctggtacaa acaaggactc attccaagtg acgctgcaac gagcaataag gattatccac
    35401 tagaaggaaa tacttggcta atgcgtggag aaacacaagg accgtatgat tatggcgata
    35461 caattttgac aaatgcagca aaacaagaac ttgtctcaaa agcaattact gttccactaa
    35521 aatctacagc tcaagcacaa atggcaaatt ttgtcgtatc aaatacttca aaaaataaag
    35581 aaaaatcagt agaacttcta gggctgctta acagtgatcc agaattattg aatgggcttg
    35641 tttgggggat cgagggagaa gcatgggaaa aagtcgatgg ttcagatgat aaaatcaaat
    35701 tgctagatgg ttatcagcca aatacacata tgtctgcttg gaatacagga aacaacaaga
    35761 ttctatatac acaagagtct attacggatg acatgattgc caaacgtgat caatcgattg
    35821 ctgatgcaga aacttcaccg attttaggat tcagcttcaa cactgacagt gtcaaaacag
    35881 aattaagtaa tatttctaat gtcatgaatc aatatttaga tggattgaat acagggactg
    35941 ttgatccaga tgaaacatta ccgaaattga aagacgcttt ggacaaggct ggctatgata
    36001 aggtattgaa agaaatgcaa aaacaatatg atgagttccg acaagaataa taatgaataa
    36061 agacaaaggg gttgtgacaa aagacgtgtc acaacctctt gttccgaata aacggtgttc
    36121 aaaagccagt ttctcggtat taagctaaat atatgtaaaa atgtggaaag ccatattcac
    36181 aaaatttttc ttactactcg aaactaagcg gctatttcac aaactcttgg taaacggtgt
    36241 tcaatcagct aaccctcggt attcgctcac aaaacgacaa aatatgagga actatttttc
    36301 cgtttgtttc tctaataatc aggtcaaggt gctgattgcc caaccttttt ttgtataatg
    36361 agaaagtata aacttaatgt ttggaggaga agatatgtta gggaaggttt tggaagagtt
    36421 atttatccgg atctgggtca ttataaaact aaccttgtat ttctggatat atacatttgc
    36481 cggcgggatc atctttggat taggtgcggc atggaaaaca gtcaatgaac tattttattt
    36541 gtacggattc gagtataaag aaatcacaat aaaaagaggc tggaatatat ataaaagaaa
    36601 ttttttaaga ggcaatctgc tatttagttt atttttatct ggtactgctt tattgagtta
    36661 caatttatat ttatctgttc aaatcaaagg gctcattttt ttagctattg attttatttt
    36721 actttttgcc ttgctctgcc tatttgctac ttatcaatac agtatgattt tagatagtga
    36781 atatacgatc tctgttccta atctgctaaa gctaagtttt atttcggttt tttccagctt
    36841 ttcgtctttt ctaaaaataa ttattggtag cggtataatt ttaggcgtga cttggcaatt
    36901 caaaggattg atcctgtttg gtgtcattgg tcttttgacg gtttggaatg gtacgatgac
    36961 aacacattgg cgtgaagaat tagataaaca gttggaaagt tatgaataag tttcggattc
    37021 tattttcaaa aggaacctta ctaaatcgtc tgttgcggaa atacgcggtt atcatgatcg
    37081 gtgtaacgat gactgctacc gttattttta gtatccatac ttgggaacaa aatcaaaaac
    37141 aggcagaaaa catgacaagt gatgccgttc aaagcacaag cagaatgctg aatgataaaa
    37201 caaccttgag taggatcatc aaaaaccagc ttgtagggaa ttccgaaaag atcgaaaatg
    37261 tgacgaccta cttaacaaaa cccatagatc agtatctgat gtatgtatac gaacagcaaa
    37321 acagtaccga tgaattagtt tctttcccaa atcaaatcaa agatctctac gcaaattatg
    37381 aagaactaag tgcgatatat atcgtgttga atcaactacc ggagtattat gaatcttcta
    37441 gagaaaacaa aggtggggaa ataaaaaagg ggacaccaaa attactagac gccttttata
    37501 tcaagttgcc tatcacacaa ggaggagctg agtcaggaag tatttttatt ggttttcaaa
    37561 agaaagaact agatatgatt ttagaaaatc taacgtcatt caatggactc tctttataca
    37621 tgatttctgg aacgacaaat cgactataca cattccatga taataaaata gctaaagaaa
    37681 cgtttgaaag acaagaaatg atcatcacag agagtctgaa gaaaaattca caattgccga
    37741 ttgacgatct ggaaaaaaac aattttgttc aacaccaaga actgtctgat gattatcgta
    37801 tattagctgt actagataaa aaaagtgtaa aaatcgaaac gggaaaaaat cttgccccgc
    37861 tgatcattgg tatcattatt ttagacaata tactgcttat ctttctttat aaaacattca
    37921 aacgatattc acagcaagta tctatcgtca tgcaggccat ggatcaaaca gcagcaggga
    37981 atttagagac gcggatcgac ataacaaaca cggaatatga actgaaggaa ctttcaatcg
    38041 gtatcaacga aatgttggat agcatcaatc agtttgtaga agacatctac aaattagaaa
    38101 tcaaacagca agacgcacat atgcgggcgc ttcaagcaca gatcaatcca cactttttat
    38161 ataatacatt agaatatatc cgaatgtatg caatcagcga aggaagtgaa gaattagcag
    38221 atgttgtgta tgcgttttcc gctttgcttc gaaacaatac gaatcaagag aaaacgatca
    38281 ctttaaaaga agaactggac ttttgcgaaa aatatgtcta tttgtatcaa atgcgttatc
    38341 ctaatcgagt agcctatcat tttatgatcg atcctgattt ggaaaagatc gaagtaccta
    38401 aatttgtgat ccagccatta gttgaaaatt attttaaaca cggtattgat tttactcgat
    38461 tcgataatgc gctaagtgtc aaagtcttgc aggaaggcaa acgggtaagg atcatcatca
    38521 aagataatgg aaaagggatg actgaaaaac ggctaaaaca gattgaagaa aaactgtctc
    38581 atcctaaagt ggaacttcac ggttctatcg gtctgcaaaa cgtgaatgaa cgtttacgtg
    38641 ccagcttcgg cagcagttat tatatgtcgc ttgagaataa tgagacaggt ggactgaccg
    38701 tttctattac attcaaggag ggatgagcgt gtatcaagtg ttattggtag atgatgaata
    38761 catgattttg aatgggttaa aaaagataat tgattggcaa agtttaggct ttcaaatagt
    38821 agcaacagca gagaatgcca aagaaggact agctgtactt gaacagcgac agattgattt
    38881 agtagtaacg gatgtgacaa tgccagaaat aaatggactg gaatttattg aagcggctca
    38941 aaaagaacgt cataattttg aatttatgat tttatcagga taccaagaat tcgattattt
    39001 gaaaggaggt atgcaattag gcgccgtgaa ttatttgatg aaacccgtga ataagttcga
    39061 attggttgaa agcctcaaga aaatcaaaac acggttagat cagcaaaatg aacaaaaaaa
    39121 tcaacaagaa atctatcaag aactgttatt cagtcaatgg ttgaatgaag aattggatga
    39181 aataagtgaa gaagaaataa taggacaaat aagtgagaag cagcatagga tagtattgat
    39241 tcaactggca agaactcatg atactcttat caatagatgg ttgcaagaac ggcaacaaat
    39301 cttttactat caacgaaatt acggagaccg tgtattgatc acgttgttgt tggaggcaga
    39361 tgaagttgat ggcttctgtt gttttgttca agaaaacttt cctgatcagg aatggctgat
    39421 cagtatcggg gaagagacag attctttgga caaaatccct gaaacattcc aacaagcgaa
    39481 agacaatcta cagttgcatc aattttatgg agataaagac gagcatgttt tttacgcagt
    39541 acaagcagaa acaaaagatc aaaccatcga tttttcaagt ttcagacgcg tgctgcaaaa
    39601 taaacaattg gaagtcgctc aaaaaatgat cactgatttt tttgaacagt ttcagttagc
    39661 agccatgatg ccggaagata tacgatattc cagtttttta ctgtttatgg agattcaacg
    39721 ggaattgatt gatctggaag atgaggaata tttacaagga atcgaaaaaa tccatcaggc
    39781 aaaaacggtg caagaacttc agcagctact tctgtcattc atcaaaaagc atcagagaca
    39841 gaaaaagtac agtgacaatg tggaaaaagt aatcgtaatc ctgcaccagc attatcaaga
    39901 accacttaca ctcaaagaag tgtcagaaaa cctgcattta aatgtcatgt atctaggaca
    39961 gctgttcaaa aaagaaacga aaaaaagttt ttcagcttat ttgaaccatt tacgtatgga
    40021 aaaagcgaaa caactgcttt tgcacagtaa tcaaaacatc aatgagatag caagtgagat
    40081 tggctacaac aacaccacgt atttctctaa attattcaag aaaatcgtcg gcagatcccc
    40141 taaagagtac cgagaaaaca gtggagaatc ataaaagaaa aagccaatgt aatgaaataa
    40201 cttataaaaa attgtctaga aatctaattt tgggctgttc ttattacttg aggcaaggca
    40261 gctttttcat gacctctcga taaacggagt tcaaaagcca gtttctcggt attaagccaa
    40321 atttatgcaa aaatgtggaa agccattttc acaaaatttt tcttaatact cgaaactaaa
    40381 cagctttttc acgacctctc cactaaacgg tgttcaatca gctgaccctc ggtattagct
    40441 caaaaaatgg caaaatatga ggaactattt tgccatttgt ctctctaata ctcaggtcag
    40501 aacgctgatt tcacaacctc tcaataaacg gtgttcaaaa gcttgcatct gcggtaataa
    40561 gaccaaccaa acgaaaaata tgaggatcta tttttgcttg gctgttctta ttacttgatg
    40621 caggacagct ttttcacaac ctcttgttaa ttatagtttg ataaggaaaa aggcaaatct
    40681 tttgcttgat atttcttagg gtgagggatg atccctaatt ggtaagagat cgtgccgcct
    40741 ttcagcaaat cttgatgggt aaaatataat ttcgtatatt ctttgtccat ccgtttgatt
    40801 ttatggataa attgttgctg cggccgatta ggcgttgctt caatcgtcaa ttcctttcca
    40861 tttgacaaaa gcaaaacagc cttgtcaatt aatggcatac cgataacata ttcaccggtt
    40921 ccaggcgtga caggataaaa tcccagacta ttgaagatat accaagcggc catacttcca
    40981 ttgtcctcat ctccagggta acccattgga gaatcgtcga aggtctgagt catcaactgt
    41041 ttaagcagtg gctgtgccat ttcaggttgt cctatataac tgaacaagta aggatagtga
    41101 aaacttggct ggtttgaaat agctaattgt ccaaattcta tcgcggccat ctcactcatt
    41161 tcgtggatct caaaaccata tcctcctggg tcaaacgttg ggggctgatt gcacagctca
    41221 atgagttttt ctttatatct ctcactgcca ccatattttc cgatcagtcc tgaaaaatcg
    41281 tgataaacag caaagctgct ttgccaagca cttccttctg cataatcttg tccccaacga
    41341 ttagctgaaa atggttctct gaacttgcca tctttatctt ttgaacgcat aaatcctgac
    41401 tcatagtcaa agatattttg atagttcaat gattgctgct gatatttctt cgtcatttcc
    41461 tcttcttcta gtaccttggc aacttggcta atacaaaaat cgctataagc atagtctaat
    41521 gtgtgattga ctgattcgtg gtagtgatta ggaacatagc catatgtcaa gtaatcttct
    41581 gttccttggc gtccataatt tggatgtttg ctttgaaccg tagcaccttt ttccattgcc
    41641 gttagaaatt caggcatcaa gtctcgtcgg atatctttta cagcagcatc tgcaatgacc
    41701 gcatcgatca atgtcccagg cataagtcca cgttcatcag gagacagcca ttttggcaaa
    41761 tagccacttt ctctatagct gttcaaaaaa ccttccagca tttcttcgta tttttctaca
    41821 gcaatcaaag aataaagagg ataaaccgtt ttatacgtat cccaaaaccc attatttgta
    41881 tagagaacac caggttttat agatcgacta gtcgtatcat agtggatttt ttgcccacta
    41941 ggatcaattt cataaaatgt ttgtggaaat aaaaacaccc gatataaatt atgataaaac
    42001 gtccgcaatt gttgttgatc atggtgtgtg agctgtatac gatcaaaata ggactgccat
    42061 tgttcttgac tgtgagcaag gtaagtctct ttgctttggt tttgttctag ttccaggtta
    42121 taagctgctt gttccgagct gataaaagat gtcccaaatc ggatagtttg tttttttgtc
    42181 tgttcaaaat acaacgggat cgagtcgcta tttttgttgg aaacagctaa ctcatcaatt
    42241 gttaaaggat attcaaatct caatataaaa taaaaagaaa aatcaggatc ttctgaacca
    42301 gcaaaatgtg tgatttttcc ttccactgtg taaggatcta taacaaagag ctgatggcgt
    42361 cccggaaagg ataaaagtaa accgctgtca tttttgctgt aatcaataga taagattccg
    42421 ccatacatgc taggtatcaa tgtagaacag atgccataac gtaattgaca gatcgacaga
    42481 tgagtcgggc aaaatatact ttcttctggg cggtaagaac tttgagcatg gaaaagggtg
    42541 ttctcctgta gttttccatt gatcggcgta aacagcatat gactgaaatc acccatccaa
    42601 ggacttggtt gatgggtaag acggtagcct tgaaaaacgc ggtcatcagg atgaaaccac
    42661 cagctgccgt tttgatccgt agtttgcggt gcaaaataat tcatcccaaa aggaacgcca
    42721 gtataaggct ggcagttccc attagagaaa tttggctgat tcgctgttgc atgtctagta
    42781 tctatttgac cgatattcat tttatttctc ctttgttgat tgtcttttga tgaatgaaac
    42841 tggaaattta ctatcggatg gtcgatcttt tgtctctaac caagtgacca aagcatctcc
    42901 cgcaagctta cctaactgtt caaaatcttg tgcgatggtt gtcagtggcg ggtcaacaag
    42961 agctgctgct tggatatcat caaatccgat aattgaaaaa tcttcaggga tatgataacc
    43021 atgctgtctc aagagattca tggtctggat agcgacaaca tcgttttcac aaacaaaagc
    43081 agttacttca tacttagtga tataagaaag caattcatct aatgtcgcgc ttttgtcatc
    43141 cagtgaagtg tggaaagtca aaccagcttt gtccaatgct tcaaggtaac caaggtaacg
    43201 ctgtcgaacg gattgtggat gagtctgcgt accaaacaaa taagcgattt ttgaatgtcc
    43261 ctgttcgatc aaataagagg cagcaagcgc accgccttgg aaattatcgg aaagaacggt
    43321 aggaaaaggt aactcataga tttttttatc taatatgacg acagggaatt ctttcaatga
    43381 aagctcaaaa agtaagtcaa gatgttgttc tgtatcaaga gcataataaa tcaatccgtc
    43441 aaattcattg attagctctt ttgctgtttt attttgtaga aagtcaagcg tcgtcatcat
    43501 gacatcaatc tgtttatctt gcatcacagg gttcaatccc ttgctaaaat caccaacaga
    43561 caaatcagcg acatatggaa gaagaaacag aatccgtcgt gtttttgtag gagaagcaag
    43621 aatctgttcc ggagaagcgg atttaacgaa actgcctttc ccgcgaacgc gataaataag
    43681 tcctaattgc tctaattcag tcaatgcacg tttggaagtg attctgctta ctttataggt
    43741 acgtgaaagt tctttttcag ttggtagttg gctgtctaca ggtaattcac cagttaaaat
    43801 agccgtttgc aaatcattaa atattgtctg gtacaatggt tgattcatat aaacacctct
    43861 taatgatata tcatattcaa ataataccat aatggatgaa aaagtaaata aaaagatatt
    43921 ttttgaaagc gtagacataa ttggctgtat gaagtataat ctttaaagat atatcaaata
    43981 gaaagaaggt tttattagtg gcttataaag atataccaaa atcggtgcag aaatttatgg
    44041 aagatatcac acaaaaatgt ggggatatcc atgcggactg ggcaaagaat tttacagcag
    44101 catttgccaa tactctgttg acaacagtca aaaagcaaaa tgacggaacc acctttttat
    44161 tgacgggaga cattccagcg atgtggttaa gagactctac tgcacaagtt cgtccttatt
    44221 tagtgattgc aaaagaagat gaggaattag ctgacatgat cagcgggtta gtcaaaaagc
    44281 aattttttta tatcaatatc gatccatatg cgaatgcctt caacgaagaa gctaacggtg
    44341 ctggccacca aacagatcat acaaaaatga acgattggat ctgggaaaga aaatatgaaa
    44401 ttgattcttt atgctaccca atacagttgg cctatcttct ttacaaaaat acaggaaaaa
    44461 ctgatcaatt caatcaatct tttattgaag gtatcaaaaa aatcttggac gtgttcaaaa
    44521 cagagcaaga tcatgaacac tctccttata catttgtacg agacacgaca agaaaagaag
    44581 atacgctgat caatgacggg cgaggtgcaa aaacagcttt tacaggaatg acatggtctg
    44641 gtttccgtcc tagcgatgat acttgccgtt atggatatct ggtaccatcc aatatgtttg
    44701 cggtggtcgt gttaggttat gtggaagaaa tttttcaaac gatactcgat gataaagaaa
    44761 ttgtacagca aacacagaaa ttgaagaaag agatccaaga ggggattcag aatcacggac
    44821 gaacggttaa tcaaaaaggg caaacgattt atgcatatga agtagatgga atgggaaacg
    44881 ccacagtcat ggatgacagt aatgtgccca atctgatcgc cgcaccttac ttgggatatt
    44941 gttctgaaca agaggaagat tatctgcaaa caagaagaac attgttgagt aaagaaaatc
    45001 catatttcta tgaaggaaag tacgcaaaag gaattggatc ctcccatacg ccagaaaaat
    45061 acgtttggcc gattgcatta gctatggaag gaatgacaac gtcagacaaa tctgaaaaag
    45121 agcggatatt gaatttactt gtagcaactg acgcaggaac acaccttatg cacgaaggat
    45181 tcgatgtgaa caatcctgaa aattatacga gagaatggtt ttcttgggca aatatgatgt
    45241 tttgcgaatt agtcatggat tactttgata ttagagtaga aaagtaggag gtatcgtaaa
    45301 tgaaaaagaa agtatatatc atttctcata gccactggga tcgtgaatgg tacatggctt
    45361 acgaacagca tcacatgcga ctgattgaat tgatagacga tctactagag ctatttgaag
    45421 ttgatccgtc cttcaacagt tttcatttgg atgggcaaac gattatttta gatgactatc
    45481 ttcaagtaag gccagaaaaa agacaggcaa tccaacaagc aatcaatgaa ggaaaattga
    45541 gaattggacc attttatatc ttgcaagatg attttcttat cagctcggaa tcgaacgtga
    45601 gaaatatgct gattggcatg gaagaaagcc gtaaatgggg aacccctgtc atgttaggct
    45661 attttcccga tacatttgga aatatgggac agacgcctca attaatgaaa caagctggta
    45721 tttcagcagc agcgtttggc cgtggtgtaa aaccaattgg ctttgataat caagttttgg
    45781 aagcagaaaa ctattcttcc caatactcag agatgtggtg gaaaggacct gatcaaacag
    45841 caatctttgg tctgctattt gccaattggt atagcaacgg gaatgagatt ccagtagaaa
    45901 aagaagcagc tcttgccttt tggaagcaaa aattagcgga tgcagaacaa tatgcctcta
    45961 cgaaccactt attgatgatg aatggtgttg atcatcaacc ggtacaaaaa gatatatcaa
    46021 aagcaatcca tctcgcaaat gaactgtttc cagattacga gtttattcat tctaatttta
    46081 ctgactatct ggaagcagtt caaaaagatg tgccagaaga cttaggctca gtagaaggag
    46141 aattgacaag tcaagaaacg gatggctggt atactttagc caatacagca tctgctcggg
    46201 tttacttaaa acaatggaat accaaggtcc agcgacagct agaaaatatt acagaaccac
    46261 ttgcgacgat ggcttatgaa gttagtggaa actatcccca tgatcaatta gattatgctt
    46321 ggaagacatt aatgcaaaat catcctcacg acagtatttg cggttgttca gtcgattctg
    46381 tccatcggga aatgatacct cgttttgaaa aagcagatga agttggaaag tatcttgcac
    46441 aagattcctt agaacagctg accgcagcaa tcgatacgac cggattccca aaagacagct
    46501 ttccatttgt cattgtaaat actgcaggaa tggataaaac tggtgaagca gaaataacca
    46561 tcgaattaga aagaaaaaga ttcgctgaag gaatacctga acaactatat caagagttag
    46621 aaaatttgcc taaacgtaag tatcatgtag aaacaaaaag tggggccact ataccagcta
    46681 ttttatctga agaaactgtc caatttggtt atgatttgcc gaaagaccgt ttccgtgtgc
    46741 catatatggc aagaatgatc aaagtgactt taccattaga aaacatgcca gcattttctt
    46801 gggaaacatt cgcgcttgta gaaggggaag cgaaagcaga agaaaaggaa acaatgattc
    46861 atcagagtgg acgaatcatc gaaaacggcc ctctacattt gacgattgaa aaaaatggta
    46921 ccattacaat ggaagatcgc aaaaacaaac gaaagttgaa taatctgcat atttttgaag
    46981 atatcggcga tataggaaat gagtatatct ttaaacaacc tttctgtgat cagccaatcc
    47041 tatccagtaa caaagaaaac tctgaagtaa aagtattagt agatacaccg gaaatagcaa
    47101 aaataagtat tctacaggaa atggaaatcc ctgtttctgc agacgagcgt ttagaaaaag
    47161 aacagcagat ggttgtggaa tttcgttatc gaaaagcaga acgatccaaa gaaaaacgaa
    47221 tccttcaaat aaaaacaatc atgacaatca gaaaagatag taaaaaaatc gactttgaaa
    47281 cgactctcga caatcagatg aaagatcatc gcttacgtgt cttgtttcct acaaagttac
    47341 atgtggaaca tcacgaagca gatagtattt ttgaagtggt gaagcgccca aaccatgtgt
    47401 ctaaaagttg ggaaaacccc acgaatccgc aacatcagca agcatttgtc aatattcatg
    47461 atgaagagta cggtgtaaca gtcggaaact ttggtttgaa tgaatatgaa gtaacagaag
    47521 acggacaaat cgcagtgacg ctacttcgca gtgtgggaga actaggcgat tggggatatt
    47581 ttccaacacc agaagcacag tgtctaggtg agcatcggtt caattacagc attgaactac
    47641 atggaccaga agaaaaattc tctacttatt tgcatgcata cgcagcacag attccttttt
    47701 ccactcaaca aatcaaacat catgaaggaa cattgatttc aaaacaacaa tatttaacga
    47761 taaaaagtga aacgtttgca attacagctt tgaaacgcag caagtttagt gataaagtag
    47821 tagtaagagg tttcaatatg tctagtcatt tagaaaaatt ggaaataact aaagacaatg
    47881 gaaaaactgt catattgaat ctgcttgaag aaccaacgaa gcaagcggtt gtcccaataa
    47941 tccaacctta tgagattcgg acaatcggat tcgaggagga aaacgaatgt atggcggaat
    48001 agaagcaggc ggaacaaaat ttgtgtgtgc ggtttcagat ggtggagaaa ttttagaaaa
    48061 attaagtatc cctaccacaa caccagaaga aacattggat caggtatttg aattttttga
    48121 tcgttatgaa ttgaagggaa tcgggattgg ctcattcggc ccgattggta tcgacaaaac
    48181 aaaagataat tacggatacg ttttggcaac tcccaaaaaa gggtgggcac aattcgattt
    48241 tcttggcaca atcaaggaac gctacaatat tcctgctgct tggacaacgg atgtcaatgc
    48301 tgctgcatat ggggaattga agcaaggagc agcacaagga aaaaatagtt gtatttacct
    48361 taccgtggga acaggaatcg gtgcaggtgt cgtgatcaat gaagaaattt tttcagggat
    48421 cgcacatcct gaaatgggac atatttgggt gaaacgccat ccagatgacc attatgaagg
    48481 tacttgtcct tatcataaag attgtttaga aggattagca gcaggaccgt ctattgaggc
    48541 acgcacaggg atcaaagggc aagatctgcc gcaagatcat cctgtttggg ctattcaggc
    48601 ttattatatt gctcaagcac tgatcaacta tacgttgacg ttagcacctg aaaagattat
    48661 tttaggcggt ggggtaatga atcaggatca tctccttcaa aaaatccgtc agcaatttgt
    48721 tgaattgatg ggtggttata tggaaacacc tcaagtaagt gaatatatcg ttcgttgggg
    48781 actgccgaat gaaagtggga ttatcggtag tttgctgtta gcagaacacg aacatcaaaa
    48841 gtaagcagac aaattacaca aaaagaacag agtcttatca ttatctgctg ataagactct
    48901 gttctttttt atttctattc ccataatcca ggtaaagctt taccgactat tttttcttga
    48961 gtaaatggat ggatcatgtg cagttctaat gcatggagca tcagtcgttc agctgttcta
    49021 ttttggtaga gagggtctcc aacgatcgga tgtcccttat gagctaaatg aactctgatc
    49081 tggtgtgtac gtccggtttc caaaacacaa tagacttgtg tttgttcaga aagtactttg
    49141 ttgacagtga catgtgtcaa ggcgctctga ccatttttat catctacgac cctttttctc
    49201 cgatcatggc gatctcgtcc aattttattg gtgatggtca tatctttggc aatttctccc
    49261 catacgatgg cttgatatct ccgatagatc attttttgtt ccaacattct tccaagtatc
    49321 ggcaagacaa atggattttt tgcaaataaa acggcaccgc ttgtttcttt gtccaagcgg
    49381 tggacaacgt aaggtcgttg ctgtttagtg tcgagataag cagctaaatg attgagtaag
    49441 gtactgtttt ctgtcggttc atttggatga gtcttcattc ctgcaggctt atccactata
    49501 atcaaatgct catcttcata taaaacacgg atgtgcttcg catctcctaa ttgaacttct
    49561 tgataattat aatctgtttc ttcgaataac aatgtgatct ggtcattgcc ctttacttca
    49621 tgatggaaca aagcaggttg ttgattgatc caaacgtttt tacgtatacg caaaaagtgt
    49681 cgaacctttc taggaactaa ccattccttt tcaagcaagt cgcgaattgt tgtttcagga
    49741 tggttcatag gtaaagtgat cgtgattttc acaagtgtac tcctttcagt gcaatcgcag
    49801 actaattgta ccatttttaa aacatattta agaaaaaact tacatttttc tgttcattct
    49861 tatgttactc tacttgcact aacgtaaaaa acatattgaa ctacaactta agcaggaaga
    49921 aaacactctt tcactatggt aaaataagac gattgaatca aatagacagg agaagaagca
    49981 aatggatttt caaagcattt tagaaaagat aaaagctgct ttgatcagtt tttggcgttg
    50041 gatcagacca tatctcggac aattccatcg atggcgaaag cgcatctgga aaaaatatca
    50101 tgtgaacaaa ttgattttgc tgattggttt gatctgtgtc ttagtgacaa gtatctacct
    50161 ctttatttta gcaaaacaag caaatgtcga aacgttaaaa tcaggattaa gccaatcgac
    50221 agtcatttat gataagaaaa acgaagaagc tggcacactg tacgcccaaa aaggaacata
    50281 tgtagaatta aacgcgattt ctccctatat ccaagatgca gtaatctcga cagaagatag
    50341 gaatttttac gagcatcacg ggtttgatat caaagggatt gcccgtgcag cagtccgaat
    50401 ggtcttgtct ggcggaacat cagggggcgg aggaagtacg atcactcagc agcttgccaa
    50461 aaatgcgtat ctgacattag accaaacgtt tgatcgaaaa gcgaaggaat tatttttggc
    50521 aatcgagatc gaaaagaagt acagcaaaga agaaatcttg accatgtatc ttaacaatgc
    50581 ctattttggc aacggggtat ggggtgtcca agacgcttcc agaaagtatt ttggagtaga
    50641 tgcaacgaat gttacgctgt ctgaggctgc tactttggca ggaatgctga aaggtcctgg
    50701 tatttataat ccgatcgatc acccaaaaaa tgcgaatgat cggaaaaaca cagtactaag
    50761 tgtaatggaa gaaaacggaa aaatcacaaa agaacaagaa caagcagaaa gccagacaga
    50821 tatcagcgcg tatcttaatg acacatacga taattcagat tctggttatc gttatcctta
    50881 ttattttgat gcagtgatcg atgaagcagt taacgagtat ggtcttgatg aagaagatgt
    50941 catgaataaa ggctataaaa tctatacagc tttagatcag gattatcaaa agcaattaga
    51001 agctacttat caaaacagtg cattattccc agcaaatgct tcagatggtg ccatggtcca
    51061 atctgcttca gtagctttag atccgaatac aggtggtgta caagcactag taggacgaag
    51121 aggagatcat gtctttagag gattcagctt tgctacacag atgcgtcgtt caccaggatc
    51181 aacgatcaaa ccattaagcg tatatacacc agcactggaa gctggataca agccttccag
    51241 tatcttgaaa gacgaaccgc aaagttatta cgatgcgcat aattttgacg ggacatacca
    51301 aggagaagta ccgatgtatc aagctgttgc acaaagtttg aatttgccta ctgtatggct
    51361 tttgcatgag atcggtttac aaaaaggata tgataaagca gaagaattcg gcctgccttt
    51421 agcaaaatct gataagtatt atggcttagc ccttggtgga ctggaaaaag gagtatctcc
    51481 attgatcatg gcgggtgctt acagtgcatt tgccaatgat ggtcagatgt atacgccgca
    51541 tcttatcacg aaaatcgttg attcgactgg agcggtgatc gtcgataaca caaacaaaaa
    51601 accaaaacag gtcatatcaa aggaaacagc cgatgaaatg accagcatgc ttcttggtac
    51661 attttcaaat ggaacagcgg ctgctgcgaa tcctgcaggt tatacgatag cagggaaaac
    51721 aggaaccaca gaaactaatt tcgatgccac aaaagcaaat gaccaatgga tgatcggtta
    51781 cacgccagac gtagtgattt cgacatggat gggttacgaa gaatccagca agctgcacta
    51841 tttggaaggg acaagtggaa atgttgtcgg gaaagtcttc aaatcagcgg cagaaggcat
    51901 ccttccgtat acttcaaaaa caggatttag tgtagcagat gcctatgcca caggaggaca
    51961 agtggtagct gcagatcaag ttggaaacac aggaaattct aatggagagt ctggaaattg
    52021 gcaagataac ttgaatcaat atggtgaaca agcaaaagaa gggctgaaaa acttcggcga
    52081 tatggtgaaa gaaggcgttc agggaattgg tgaagcggct aaagacctct ggcggaaata
    52141 tcaaggagag tagccccttt gaaaacgaat aaccccatgt tacaatagag agtaactact
    52201 aaaagaaaag agggcataac atgtcaaaca tctacgatag cgcgaatcaa atcgaacgtg
    52261 agatccgtga attaccagaa ttcaaagcgt tgaaagaagc gtacgagaaa gtacaagcaa
    52321 acgaagaagc acacaaactg ttcaaagact tccaagcgat gcaaatcgaa ttacaagaaa
    52381 aacaaatgag tggacaagag ttcagcgacg aagatgcaat gaaagcacaa gaaatggctg
    52441 tgaaaatcca atcagaagaa gtaatcaatg acttgatgca aaaagagcaa ggcttcagta
    52501 caatcatcaa tgacttgaac cgtatcatca tgacaccagt tcgcgacctt tacagcgaat
    52561 aaaactattt tatgaaacaa cagagggtgt gaaagagacg ttcggccttg agcgatgaca
    52621 aaattgcttc ttattgccga taggctgtct cttgaacacc atttaccaag aggtcgtgag
    52681 cctaccgttt aactctgagt tacaaggaac aatttcaaaa aacagcttct catgtttgat
    52741 gactattgtg acttgtgacc gaggagttgg caggagaaca ccgtttattc agaaatagga
    52801 gctacgacaa aagtcttgtc atagctccta ttttttgtca ttcatttttc aagcggtaga
    52861 aatagccttt ttgagctaac agtttttcgt aagtacctat ttcaacgatt tttccttcat
    52921 cgatcacaac gattttatct gcctctttga tcgttgaaag ccgatgagcc acaataaaag
    52981 tagtgggtgt ttctgtcagg taattcaggg cttgctggat ttttttctct gactggttat
    53041 ctaaagctga ggttgcttca tctagcaaaa tgatttttgg ctgacgtatc aacgctcgtg
    53101 caatggagat acgttgcttt tggccaccag acagcttgtt gccatgttcg cctactaagg
    53161 tatccagacc atcaggaagc tggtcaatca gtgaagaaag ttgcgcagct tcgatgactt
    53221 catctaaacg ttcttttgat acgtttttca agccataagt gatgttctct ttgatcgaag
    53281 cggagaacaa caaagtggtt tgaggaacaa cggctaaata atttcgcaca cttcgcatat
    53341 ccagttcatc caaaggctga tcatctagaa aaatttttcc tgaagtcggc tggataaatc
    53401 caatcaataa gttaaggagt gtagattttc cagagccaga tggcccaaca aaagcaattg
    53461 tttctccagc tttcacctct agagaaaaat gatcgataat cggtttgagt gagtccggat
    53521 aaagaaaaga aacatcctca aaacgaaaat ttccatccaa atgttggatt tttttcttac
    53581 cgtgataagt ttctacatcg ccagcaatca acagttcgcc aatagaagtg atagattcca
    53641 tgccttttga aatcgtagga atcagcgtga tcattccaga aacggaattg acgatcgtcg
    53701 tgaaatacgt ctgatacatc acgacatcgc cgggttggat gcgcccttgt aaagctaata
    53761 tcccagtgaa aatcaaacaa atcagctgaa atatctgaaa actagcccag ctgactgatc
    53821 cgaaaacaga ttgcaggata tctaagtgga atcctttttc atagatttga gtaaaatgct
    53881 tatttaagcg agttgtttcg tgtttttcta acccatgagc tcgagcaatc ggtgctaatt
    53941 cgatcacttc gatcactttt gcagaagtct cttccatttc ttgcctgaag tctgagttgg
    54001 actgtctgat ccttttacgg aaaaagagag taacgaatgc ggcgagagga acagtcaata
    54061 agaaaaacat gaaaacaaca ggacttttat aaagcgtgat acctaaagct acgaataaat
    54121 tgacagcgat atttaatccg ctgacaaata cttgagagga caacgtttgg atagcttcta
    54181 catcacggat gatctttgac tggattcgtc cagattgcaa ttctttttga taaggaatag
    54241 atagttcttg gattttcttg ataagggtcg ctcttagtcc agcttctact tgacggatcg
    54301 aagtgctatg gtattttaca tgaaaataat tcgtcaatat attttgacca atcagacaag
    54361 ccatcaaaaa agcgttgaaa taaatggcct gcaactgaga agaatctttc gatgaagctg
    54421 cattgattac attggcaata gcgatcggca ttaaccaaga tggaagatgt ttgataataa
    54481 aaaagagtcc tgacatggca aaacgccaat aatttccctg ataaagatgc agcaatgtct
    54541 gaaaagcatt tgcatgatat ttttgataac ttttctgtat tcgttcctcc ataatcttct
    54601 ccttaacaat cttggtaaat aaactgcaag tgttctattg cttcaggaac ataaatctca
    54661 ggagtagctt caagagtggc gtacattaga ggacgctcat atttgatata atgcagtacc
    54721 gggcggaaat ctaaacagcc cttgccaaca ggtaccatgc ggatctttcc attttccatg
    54781 atatagtctt tgagatgaag cgctgtaata taaccatgta aattgtccaa ggcacctcgg
    54841 atcacttttt cctgatcagc atgattttct ttatgcatca aattcgcaca atctaaaatg
    54901 atttttaaat ttggtgaagc tacttcatcg attaaacgtt tagctaactg atagttatag
    54961 attggatgat tgatccctgc ttcaacagca acagttacgc caaaattttc tgcatgagcg
    55021 actaattcaa tgatagaatt tctggcgatc tgataagctt cttcagtaaa attttctttt
    55081 gtgtaaccga cagagacact accggtttcc gtagcaatca tcttggcttg ataggcagag
    55141 cacagagcaa tttgctgttt gaaagttgcc aatgcttgtg cacgaacttc ttgatttttg
    55201 ctggataggt tgatatagca tcctaaaatg gaaagttcga tattgttttt ttggaagtat
    55261 tcaccaaaat aggcagcact cccaagtgtc atgtcagtta atttaagttg ttcaggaaat
    55321 gctttttgtg gtgcaaattg gatatgggac aagtgaagct gtgatacttt ttgaacgatt
    55381 tcttcacgat ttcgaacatc taaatcgtgg gcacggatgc ctaaatttaa tggaaacatc
    55441 tcaaactcct ttttgtaaat aattagcaag aggcagacgg gattctttta tcaattcaac
    55501 aattattcgg gcaactttat cagcgccttc tggactaaag tgggtattgt cttttagtcc
    55561 ttctgggtaa tttttacaag tatcaggtat aagatgcagg tagaattttt ttgtctcttc
    55621 ttttgagaag gtctggaaca attcttgtga tcttgagaac acatctaaaa gaggaatatg
    55681 gtgtttctca gcaaatgcac gcatcgcttt tggatagtct ccaagtatat ctggattcaa
    55741 tgttccgttt tctaagtaat ctcgtcgcgt cacagatgtc agtaagacca tttggacgtt
    55801 cgcttttttt gcgcattcaa aaaatatctg cagatttttt aaatagctac cgaagggaag
    55861 cgtacctctt tcttcttgga ttttctggtc gttatgtcca aattgtacaa ataagaaatc
    55921 attcggctga aactgtgaag ctagacgggc taaccttcct tcctggataa atgatttggt
    55981 gctgcgtcca ttctttgctt ggttattgat ctggatctcg ggtgaaaaat aagcagagat
    56041 tttttctccc caacctgtct cgggacgctt gttttcatct ttttctgccg cagtagaatc
    56101 accggcaata tggatagtag gcatgttatc tcttctttca taaaggttta taggcaatga
    56161 agcagaacag caagagaaga agacggagca aatttggtcc gtcttcttct ctaaaacgtg
    56221 gataaacgga ttccgttatt tcactatcct gtttttgatt cgttactgtt actattaatc
    56281 aattaggcaa aaaagccaaa gtaattttta gcatttgtat aactaatcgc ttgaactaga
    56341 cttcctaaaa gttgttcatc ttcaggtgct tctcctcgca tgaccatttc gcctaaaaat
    56401 tcacaaagaa cacgacggaa atattcatgt ctagtgtagg acaggaaact tcttgaatct
    56461 gtcagcatgc cgacaaaatt ccccaaagca ctaccatcag caaattgtgt caattgatcg
    56521 cgcataccag ctcgagtatc gttgtaccac catccagaac ctaactgcag ttttccattc
    56581 tgttcttttt ggaaacagcc catcaaagct aaaatcgcag gatagtcatt cggattcaat
    56641 gaataaagga tcgtctttgg caactgatcc gtttcttctg cacggttcaa taatttttgc
    56701 agaggaatag tcaaagaaag atcattcatt ccatcatagc cggtatcggg ccctaatttt
    56761 tccaacattt gtgtattaca gttacgataa gcatggatgt gcagctgcat cgtccaatta
    56821 tgagaatgat agaagtgggt caaatgtatc agtgtttctg tacgataagc atcgatttca
    56881 tctgaagata atagtttgtt agctcgtgct ttttgaaaaa tctcttctaa tactgtttca
    56941 tccgcttctt tatagcgtaa gatgtctaaa gcgtggtcgg aaacacgaca tccatgttga
    57001 tggaagtatt ctatccgctg ccctaagact tctatcagtt cttgataggt ggtgatcgtt
    57061 tggtcagctg cttcttccag tagtgtgacc catgcattaa atccttcttt ttcaatattg
    57121 agtgctttat ctggacggaa tgaaggtaga accttcaaat ctggttcttc atgagcaagc
    57181 aactgatgat agtgcaagtc gtctgcggga tcatctgttg tacaaatcac ttctacattc
    57241 gatttttttg cgatttcccg acggcgaaaa tcaggagctg ccaaaatttc atttgcttgt
    57301 gtccaaattt tctctgcatt ttcttcattt atcaacaagt caataccgaa gaaacgcttc
    57361 aattcaagat gtgaccaact gtaaagcgga tttccgataa tttttggtac ggtttgacac
    57421 caagctaaaa atttttcaaa atcagaagca tttcctgtga ttttttcttc aggcacgccg
    57481 caagctcgca tcaagcgcca tttgtaatga tcgcctccta accaagcttc tgtcagattg
    57541 ctgaaattgt tgttttcgta aatttctttt ggatctaagt gacagtgata atcaataatg
    57601 ggcatgtttt ttgcgtagga atggtaaagc tttttcgccc aatcgtcttt taataaaaaa
    57661 tcatcagata aaaacataaa tcttccttct ttctttttat actgttttat gaaaatcaaa
    57721 gtgttactcc gtttttaaaa atggcaattt ctcgaacgtc attttgttca tttttcgttt
    57781 cttctccact agctacagcg atgatttttt gaacaaacga ttctaagatt acttccattg
    57841 gttgttctaa taagacgcct gcattaaaat ccatccaatg acctttgcgt tcaaaaagag
    57901 ttgtattcgt agataccttg accgttggga catatgagcc aaacggagtc cctcttcctg
    57961 tggtaaacaa gaccaactgg caatcggatg aagcaagagc agaggcagct acaagatcat
    58021 tcccgggagc ttgcaataaa ctaagccctg gttttctgat tttttcgcca tattgtagca
    58081 catcaacgac aggagagctt cctgattttt gcgtgcagcc taaagactta tcttctaatg
    58141 tagtgatccc acctgcttta ttaccaggag atgggttttc gtaaactggt tccccgtaag
    58201 acaagaaata ctgtttgaag tcattgatca agtcaacgat tgagtcaaaa acttcttcat
    58261 tttccgctcg tgccatcaat acttgttctg cgccaaacat ttctggcact tctgttaaga
    58321 tcgtgctgcc tccttgagca atcaagtagt cagaaaatgc accaagtaaa ggattagcag
    58381 ttattcccga tagaccatca gaaccgccgc atttcaaacc gacattcaac ttagagagcg
    58441 ggactgatat gcgctggtca ttttccgccg ctgttaaaag ctcttctaat agagccacac
    58501 cttgttcgat ctcatcgtaa acatcttgag ccactagaaa tttcactcgt tcttcatcat
    58561 attcacctaa aatttttttg aattcaggaa ctgtattgtt ctcacaacct aatccaaata
    58621 ctaaaactcc tccggcattg ggatgattta ctgcgtccgc taaaatttct cgagtatttt
    58681 gatgatcttt tccaagctgc gaacagccat aaggatgctt taaaatcgta atatggtcaa
    58741 actgtcccag atctggatgt ttttccttga actgtttgac gatcagttca gcaatgccat
    58801 tcacacaccc aacggtaggt actaaaaaca aatcattacg aatccctact tttccatttt
    58861 ttcgcaagta accttgaaat gttcggttct caaaaaggta atgacttgga tggacatctt
    58921 tttcatatcg ataatccaat tcaccagaaa gattggtttt tacattatga gtgtgcagcc
    58981 attgaccggc agttacgtct gtcgtcacat gaccgattgg atagccatat ttgatcacgt
    59041 tgtctccagc tttcaaatca cccaatgcaa tcttatgacc ttgcgggata tcttcaagag
    59101 tggtgatcgt ttgctgatca atcgtcagtt gtgtttgttt tggaataggg gttaatgcga
    59161 cgaccacact gtctcgtcga tttaatttca ttgttggttg cagggtttca agcatggacc
    59221 atcgttcctt tcatttcttg taatactgac cggacacctt tttgatcgat ctttgcagct
    59281 atttcttgaa ctgtatctgt cacattcggt atttgagaca aatcttctcc ccaaagcgca
    59341 gcgttttgca ataatcgagg aatagctgtt ccattatcag accaagcatc agtaaattcg
    59401 cttatcgtct tttcatcatc atgtggcttt acttgatcgc ctcgatacat aagaagtagc
    59461 gcagctagtg aaacagtgat taacggtggt acctgatttt gttcttcgat atacttcttg
    59521 aacactggaa gcagacgggt gctgaacttt gaaacactat ttaatgaaat cgcttctaat
    59581 ttgtgatggg caaaaggatt caagaaacgt tctttgattt ggtcagcata aatggctaat
    59641 tcatctttag gtaaagaaag cataggaatc aattctagat tgaataactg atcaacatat
    59701 gcggagaata aaggatcttt cattacttct tctacagttt ccaattccgc caacaaagca
    59761 agagggacca tcgctgtatg cggtccatta agcaaatgca ccttacgttc acgataagga
    59821 gtcatatcat ctgtgatgat gacattcaag ccagcctgtt tgaatggtag ttgattctcc
    59881 aattctttag gcccttcgat cacccaaagc atgaatggtt ctgctttgac catcaattga
    59941 tcttcatagt tatgccgttg attgaaaaca cctgcatctt cgcgaggata acctgggacg
    60001 atgcgatcaa ctaaactaca acagaaaaca ttttcttttt ctaaccactc aacaaaagca
    60061 ggttctaaat cccatttttc tgcgtagcgt aaaatgattt cttttagacg ttcgccgttt
    60121 ctgtcaatca gttcgcaagg aatgatggta aagccagctt tgtttaattt gaaacgtcgg
    60181 tataaaaagg ctgtcagttt gcccgggagg ctctgtgggg gagtatctgt taaattatca
    60241 gtctccacga attggattcc tgcctctgtt gtattggaga cgatataggc caagtcgtca
    60301 ttttctgcta gagatagata agcatcccat tcatcataag gattgatcat ttgttctact
    60361 acggtgataa tctcggaagt atcaacgatt tcgccattgc tgagtccttg taaaatgacc
    60421 gtatataaat tatcttgctg gttcatcata tttccaagac cttgttctaa aggttgaatg
    60481 attgcaacat tacctttaaa caattgttgg ttattcagct gctgaatctg ccaatcaata
    60541 aatccacgca taaaattgcc ttcaccaaac tgaagaactt ttacaggtgc ttcgtttttt
    60601 ttcactaatt cttttgtcag ttttttcata taaacttcct ttctgtgtta tgcacacgtt
    60661 aacattttta tataaaatta cttgtagttt acaagtaatt gatcgactca cgaaggatca
    60721 acaaggtgga gagctcaatt ttagattgtt cgatcgtcct gccttcgatc aaatctagta
    60781 aaagctttgt tccttcttga cacatttctt tcaaaggctt acgtacggtg gtaagcggcg
    60841 gattgagata ggagacgact ggagaatcat cgaacccgat cagagaaatg ttctggggaa
    60901 cacggaggcc agctaaatgt acagctctca atgcaccaat cgctatgtcg tcgttgccgc
    60961 aaaaaaccag agtaggcagg tcagaaagtg ataataggta ttccatgttt tggaggccgc
    61021 tttctaagct gtaatctcct gttaaaaaat aggctggatt gacagggatg ctcgcttttt
    61081 ttagactttt caatacccct ttttcccgtt cttttgtcga acgaaaattt ttctttccgc
    61141 caatataagc gatcttcgta tgtccgcatc caataccgta atctactgcc tgcataacac
    61201 cggccgcatc atcggcagtg acgttgataa ccgctggatc attgatgtga cgattgacaa
    61261 caacgaaagg aaattctttt tctttgagat agtcgatgaa aggctgatca tcatcacttt
    61321 gactcataac gatcagtcca tcgtagcgtt gcaggttgat ttctgttaaa tcaacaacat
    61381 cgatactttc aacagataaa ctgtaatttg tgtctaatac tgaatggatg cctgcaatgg
    61441 aatctgctag gaaatttgat gaagttcctt tatgcaaact tgagaaaaat agtccgatta
    61501 gatatttttt ttgattcact aaaccttttg cactatagtt tggcgaataa ttcatttcct
    61561 tagctagttg tcgtattttt tgttttgttt cattttttat taaactgctg ttattcaaag
    61621 cacgagaaac tgtcgtatga gaaacgccag ctgctttagc gacatctttg atcgttacca
    61681 ttccatcatt ttccttactt tttcactttt tgttaacgca ttcatcatat tacgataact
    61741 acgataagtc aatagatctt tgatatgaaa agattgcaca cgttaaaaat tacttggatt
    61801 tttattttta attatgctat tcttgaaagc ggggaatgct catttaatta gatgagtatc
    61861 aattttacag aaaaatgaaa gccttttatt ttaaaaggag gagaggaaga aaatgaagaa
    61921 aagtacagta ttttataaaa tatttttacc gatgttttta ttaggagccg tacttgtcat
    61981 aggatttagt ttattcattt accagaatac ctatgaatcg atcgaatcga gttatttaac
    62041 agacaaaaag aatttgctca ggcaagttaa aactaacgtt gaatggaaaa ttagaacgat
    62101 tgaatattcg ttttctacgt atggttcgac caaaaatttc tcggacatat tcaagcatcc
    62161 gctgacatat acagattact ccacttattc tgaaatcagg aaagaactaa attttataga
    62221 aacgattgtt atggatgata ataattatga tttagtcagt ttggctggta aatggggagt
    62281 aatcaatgga tcgttgagtc aactgaccga agaggaaatt gaaaagtatc aacagaagta
    62341 cataaataac aaaaataatt tattttggca aaagaaaaac aaaggaatcg aaatggtgat
    62401 ttctttgcca atgtttgaaa aagaaaaata tgcattggga attgctacga tagagaaaca
    62461 tacaatagaa caagttgttg gtgaaagtgg agaagacata ctttctattg aaaacgaagt
    62521 tggcaacctt tttactactc agacagtaaa aaataaagag caaccaactg tattaacgga
    62581 cgtggatcta gattacaatc agctgaaagt gataagagaa aaagataaaa cgtatatagt
    62641 actgaaatca gattataata attggattta cagattagaa gttgactcta gtgcaattgg
    62701 cgcaatgatc cgcaatctga gaatcgggct agttgcagtg tctttaacac tggtaggact
    62761 cattggtctt ctttcctact tattttcgga cagatttgcc cgtccgattt ctcaaataca
    62821 ggaacgtctg aatcttaaat caaaaggatt cagtgggaaa gaactagatt tggtcgctca
    62881 atcagtcagt cggatcatag gagaaaaaga agcgttaagt gcacacttag ttacccaaaa
    62941 gccacagctg gaaacgctat ttgtacttag tttatttaga aatcgagtag aaaggcgaga
    63001 actagatcag cggttgcagc aatttggtta tagttctcaa aatgactgct attatacagc
    63061 tttggttcaa atcgatctgt tagaagatag tcacggtggg gagcgtgatt tgctactttt
    63121 agcgatcaac aacatgatca cggaaattgt gccaaaggaa aatcgaatgc tgccgatcgt
    63181 tttgaatgaa gaaatgcaag ccactattta tcgaatggat aaagatgatg cagaagcaag
    63241 caaaaaagtc atggagtatt gccgactcat tcaaaaacgt gtaaaagaat acttgaattt
    63301 aactatcagt ataggcatca gtaaccggtt cgaaacgctg aatgaaagca agcaatcggt
    63361 tgatcgggca aaagaagccc tgtattatcg tgtaaatact ggtccaagtt caattatttt
    63421 ttatcaggaa atcgttcctg ctgctcatga aaaaacactt attagatatc cgatagaaga
    63481 acaaaatcga ttgtttgaag ccattcgttc tggaaatcag gaagtgaaga atttagtaca
    63541 tgaattgatt gatgcattgt ttgcacaaaa taaaaatcct ttgagtcggg aagtagtttc
    63601 ggtccgcttg atcaatgaac tcgttcaatt aggacagtta ttgggagtag attcaaaaaa
    63661 ctttgataac atgaaacaaa tttatgtaaa agcactgaac aattatcatc ccaaagaact
    63721 agaaaaactt gttttagaac aattagttct gccaatcaca gaatgctctc aagcaatcac
    63781 tgatcatgaa tttaaaaatt tgtcggagaa tattatgcat atcgtctaca cagaatatga
    63841 tcgtgatctg tctttagatg tgatcgctga taggttgcat tataacccta actatttaag
    63901 taatgtattt aaaaaggaaa caggagaaaa atttggtgat tttgttcaaa atcatcgttt
    63961 agaggtagca aaaaaatggt tgaaagagac taacctctcc gtaaaagaga ttgcagaaag
    64021 attgcaatat cgaaatccgc aaaattttat tcgtttcttt aaaaagaagg aatcgattac
    64081 gccgggagaa tatcgaaaaa aatatttgtg aatcaagagg ctgtttttct tgtggactga
    64141 ttcaagcaat aaaagaagtt agaataaccc tagtatttgt atttatggat agttaaattt
    64201 caaggctaag cacgcaaaaa gaactgtgac aaaagtcttg tcacagttct tttttacgaa
    64261 taaatggcgt tcaagagaca ggctatcgac aataaggcat aattttgtga aatatgggaa
    64321 gctatttttc aaaatcgctt cttattgctc ctgtgggtta ctcgtcgcaa tgtttgtgag
    64381 agtttttatc atcgctcaag gctgaacgtc tctttcacaa cctctgatac acggtgttcc
    64441 aggagtagat tcttcgaaaa ataagccgaa atttcacaaa aatttaagaa gcaattttcg
    64501 taaatgcctt cttatttctt gaagctgacc acttctgaca caactaccat t
//
LOCUS       DASHRQ010000009        62382 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.009, whole genome shotgun sequence.
ACCESSION   DASHRQ010000009 DASHRQ010000000
VERSION     DASHRQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.009"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            287..694
                     /locus_tag="U2M88_000598"
     CDS             287..694
                     /locus_tag="U2M88_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373003.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="U2M88_000599"
     CDS             708..1109
                     /locus_tag="U2M88_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373004.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="U2M88_000600"
     CDS             1111..1482
                     /locus_tag="U2M88_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373005.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1820)
                     /locus_tag="U2M88_000601"
     CDS             complement(1518..1820)
                     /locus_tag="U2M88_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373006.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1916..2710)
                     /locus_tag="U2M88_000602"
     CDS             complement(1916..2710)
                     /locus_tag="U2M88_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEM8373007.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISATNSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
     gene            3356..6163
                     /locus_tag="U2M88_000603"
     CDS             3356..6163
                     /locus_tag="U2M88_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEM8373008.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            6354..6845
                     /locus_tag="U2M88_000604"
     CDS             6354..6845
                     /locus_tag="U2M88_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8373009.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            6948..7937
                     /gene="manX"
                     /locus_tag="U2M88_000605"
     CDS             6948..7937
                     /gene="manX"
                     /locus_tag="U2M88_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEM8373010.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            7970..8773
                     /locus_tag="U2M88_000606"
     CDS             7970..8773
                     /locus_tag="U2M88_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEM8373011.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            8794..9708
                     /locus_tag="U2M88_000607"
     CDS             8794..9708
                     /locus_tag="U2M88_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEM8373012.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            9791..10165
                     /locus_tag="U2M88_000608"
     CDS             9791..10165
                     /locus_tag="U2M88_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEM8373013.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            10304..10549
                     /locus_tag="U2M88_000609"
     CDS             10304..10549
                     /locus_tag="U2M88_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEM8373014.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            10721..11785
                     /locus_tag="U2M88_000610"
     CDS             10721..11785
                     /locus_tag="U2M88_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEM8373015.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            12031..12456
                     /locus_tag="U2M88_000611"
     CDS             12031..12456
                     /locus_tag="U2M88_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEM8373016.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            12449..12898
                     /gene="nusB"
                     /locus_tag="U2M88_000612"
     CDS             12449..12898
                     /gene="nusB"
                     /locus_tag="U2M88_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEM8373017.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            12995..13849
                     /locus_tag="U2M88_000613"
     CDS             12995..13849
                     /locus_tag="U2M88_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEM8373018.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            13850..15187
                     /locus_tag="U2M88_000614"
     CDS             13850..15187
                     /locus_tag="U2M88_000614"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEM8373019.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            15192..15416
                     /locus_tag="U2M88_000615"
     CDS             15192..15416
                     /locus_tag="U2M88_000615"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEM8373020.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            15418..16308
                     /locus_tag="U2M88_000616"
     CDS             15418..16308
                     /locus_tag="U2M88_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEM8373021.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            16317..17135
                     /locus_tag="U2M88_000617"
     CDS             16317..17135
                     /locus_tag="U2M88_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEM8373022.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            17242..17691
                     /locus_tag="U2M88_000618"
     CDS             17242..17691
                     /locus_tag="U2M88_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEM8373023.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            17709..19391
                     /gene="recN"
                     /locus_tag="U2M88_000619"
     CDS             17709..19391
                     /gene="recN"
                     /locus_tag="U2M88_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEM8373024.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(19446..19562)
                     /locus_tag="U2M88_000620"
     CDS             complement(19446..19562)
                     /locus_tag="U2M88_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEM8373025.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(19635..20591)
                     /locus_tag="U2M88_000621"
     CDS             complement(19635..20591)
                     /locus_tag="U2M88_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEM8373026.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            20762..21130
                     /locus_tag="U2M88_000622"
     CDS             20762..21130
                     /locus_tag="U2M88_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEM8373027.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            21300..21731
                     /gene="mraZ"
                     /locus_tag="U2M88_000623"
     CDS             21300..21731
                     /gene="mraZ"
                     /locus_tag="U2M88_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEM8373028.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            21752..22711
                     /gene="rsmH"
                     /locus_tag="U2M88_000624"
     CDS             21752..22711
                     /gene="rsmH"
                     /locus_tag="U2M88_000624"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEM8373029.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            22716..23117
                     /gene="ftsL"
                     /locus_tag="U2M88_000625"
     CDS             22716..23117
                     /gene="ftsL"
                     /locus_tag="U2M88_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEM8373030.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            23118..25310
                     /locus_tag="U2M88_000626"
     CDS             23118..25310
                     /locus_tag="U2M88_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEM8373031.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKSGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            25336..26298
                     /locus_tag="U2M88_000627"
     CDS             25336..26298
                     /locus_tag="U2M88_000627"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEM8373032.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            26312..27673
                     /locus_tag="U2M88_000628"
     CDS             26312..27673
                     /locus_tag="U2M88_000628"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEM8373033.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            27699..28787
                     /gene="murG"
                     /locus_tag="U2M88_000629"
     CDS             27699..28787
                     /gene="murG"
                     /locus_tag="U2M88_000629"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEM8373034.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            28802..30022
                     /locus_tag="U2M88_000630"
     CDS             28802..30022
                     /locus_tag="U2M88_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEM8373035.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            30178..31503
                     /gene="ftsA"
                     /locus_tag="U2M88_000631"
     CDS             30178..31503
                     /gene="ftsA"
                     /locus_tag="U2M88_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEM8373036.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            31526..32767
                     /gene="ftsZ"
                     /locus_tag="U2M88_000632"
     CDS             31526..32767
                     /gene="ftsZ"
                     /locus_tag="U2M88_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEM8373037.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            32784..33461
                     /locus_tag="U2M88_000633"
     CDS             32784..33461
                     /locus_tag="U2M88_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEM8373038.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            33475..34074
                     /locus_tag="U2M88_000634"
     CDS             33475..34074
                     /locus_tag="U2M88_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEM8373039.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            34089..34376
                     /locus_tag="U2M88_000635"
     CDS             34089..34376
                     /locus_tag="U2M88_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEM8373040.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            34425..35207
                     /locus_tag="U2M88_000636"
     CDS             34425..35207
                     /locus_tag="U2M88_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEM8373041.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            35228..35935
                     /locus_tag="U2M88_000637"
     CDS             35228..35935
                     /locus_tag="U2M88_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEM8373042.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            36197..38995
                     /gene="ileS"
                     /locus_tag="U2M88_000638"
     CDS             36197..38995
                     /gene="ileS"
                     /locus_tag="U2M88_000638"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEM8373043.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(39044..40570)
                     /locus_tag="U2M88_000639"
     CDS             complement(39044..40570)
                     /locus_tag="U2M88_000639"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEM8373044.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(40585..41232)
                     /locus_tag="U2M88_000640"
     CDS             complement(40585..41232)
                     /locus_tag="U2M88_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEM8373045.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(41416..41745)
                     /locus_tag="U2M88_000641"
     CDS             complement(41416..41745)
                     /locus_tag="U2M88_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEM8373046.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            41922..42650
                     /gene="sfsA"
                     /locus_tag="U2M88_000642"
     CDS             41922..42650
                     /gene="sfsA"
                     /locus_tag="U2M88_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEM8373047.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            42666..43679
                     /locus_tag="U2M88_000643"
     CDS             42666..43679
                     /locus_tag="U2M88_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEM8373048.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            43679..44956
                     /locus_tag="U2M88_000644"
     CDS             43679..44956
                     /locus_tag="U2M88_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEM8373049.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            45019..47721
                     /locus_tag="U2M88_000645"
     CDS             45019..47721
                     /locus_tag="U2M88_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEM8373050.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            47873..48190
                     /locus_tag="U2M88_000646"
     CDS             47873..48190
                     /locus_tag="U2M88_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8373051.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            48220..48540
                     /locus_tag="U2M88_000647"
     CDS             48220..48540
                     /locus_tag="U2M88_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEM8373052.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            48648..50108
                     /locus_tag="U2M88_000648"
     CDS             48648..50108
                     /locus_tag="U2M88_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM8373053.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            50176..50397
                     /locus_tag="U2M88_000649"
     CDS             50176..50397
                     /locus_tag="U2M88_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373054.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            50428..50610
                     /locus_tag="U2M88_000650"
     CDS             50428..50610
                     /locus_tag="U2M88_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEM8373055.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            50610..51023
                     /locus_tag="U2M88_000651"
     CDS             50610..51023
                     /locus_tag="U2M88_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEM8373056.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            51146..52327
                     /locus_tag="U2M88_000652"
     CDS             51146..52327
                     /locus_tag="U2M88_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8373057.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(52398..52562)
                     /locus_tag="U2M88_000653"
     CDS             complement(52398..52562)
                     /locus_tag="U2M88_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373058.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            52716..52789
                     /locus_tag="U2M88_000654"
     tRNA            52716..52789
                     /locus_tag="U2M88_000654"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52750..52752,aa:Arg,seq:cct)
     gene            complement(52858..53997)
                     /locus_tag="U2M88_000655"
     CDS             complement(52858..53997)
                     /locus_tag="U2M88_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM8373059.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(54296..54931)
                     /locus_tag="U2M88_000656"
     CDS             complement(54296..54931)
                     /locus_tag="U2M88_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373060.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(55044..55679)
                     /locus_tag="U2M88_000657"
     CDS             complement(55044..55679)
                     /locus_tag="U2M88_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HEM8373061.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(55713..56174)
                     /locus_tag="U2M88_000658"
     CDS             complement(55713..56174)
                     /locus_tag="U2M88_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEM8373062.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(56304..56735)
                     /locus_tag="U2M88_000659"
     CDS             complement(56304..56735)
                     /locus_tag="U2M88_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEM8373063.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(56753..57073)
                     /locus_tag="U2M88_000660"
     CDS             complement(56753..57073)
                     /locus_tag="U2M88_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8373064.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            57372..58148
                     /locus_tag="U2M88_000661"
     CDS             57372..58148
                     /locus_tag="U2M88_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEM8373065.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            58163..58366
                     /locus_tag="U2M88_000662"
     CDS             58163..58366
                     /locus_tag="U2M88_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8373066.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            58382..58720
                     /locus_tag="U2M88_000663"
     CDS             58382..58720
                     /locus_tag="U2M88_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HEM8373067.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            58707..58886
                     /locus_tag="U2M88_000664"
     CDS             58707..58886
                     /locus_tag="U2M88_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373068.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(58929..59399)
                     /locus_tag="U2M88_000665"
     CDS             complement(58929..59399)
                     /locus_tag="U2M88_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373069.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            59486..60184
                     /locus_tag="U2M88_000666"
     CDS             59486..60184
                     /locus_tag="U2M88_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEM8373070.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            60362..60703
                     /locus_tag="U2M88_000667"
     CDS             60362..60703
                     /locus_tag="U2M88_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373071.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            60696..61367
                     /locus_tag="U2M88_000668"
     CDS             60696..61367
                     /locus_tag="U2M88_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEM8373072.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            61373..62059
                     /locus_tag="U2M88_000669"
     CDS             61373..62059
                     /locus_tag="U2M88_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373073.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            62062..>62382
                     /locus_tag="U2M88_000670"
     CDS             62062..>62382
                     /locus_tag="U2M88_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8373074.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPID"
BASE COUNT        20868 a        10379 c        12884 g        18251 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaatcc
     1741 cacaccttat aaaaatcttt gacaaatcta ttcgaaggat cgaatttaaa ccataattgt
     1801 gtttttcctt ccattaacat ataaagttca cctcaaaaag agtatacgaa caaatgtttg
     1861 tattgtaaat agaaaaagat gagctaaatt aaatgcccat cttctttgat agatattata
     1921 aatctaataa ttcttgtttc tttttattaa attcttcttc tgtaatgatt ccatcatcca
     1981 atagttcttt atattttctt atttctgaag ctgaggaaat tgttgatgta ttggaatttg
     2041 tagcagaaat atttcttctt tttaaattat aaataatttc gtttaaagaa tcacttatcc
     2101 tatcaacagt atctttatca actgcaacat tcaatacttt accaagagta tcaatagtta
     2161 atgtacccaa cagcactcct ctttttttac taatattatt taaattgtct aatataatac
     2221 tttgtacatt attgcctatc attttttgtt gagctacaat taaacgttta ttagttaatg
     2281 cgtatgcata attattgtca tgcttagttg atgagatata attatgcaat cctacaaagc
     2341 agaagattac gtattcgtca ctgttaagtt gatctgatat taagttaaaa tgatgagttg
     2401 tccatttttt agtcattcct ttaccgtagc cgtatttaag acaaaattct accatctctt
     2461 cagtggttct cattctatca ttacctatat catgattgtt ttgtgcagta tgatcagata
     2521 tgtttaattt tcttttttct gatggatcct tatctaaatc ttctaaattc aaagatctta
     2581 ttattttata aggagtagtc ataccgtaac cagctttttt catacaattg ccgcaaacaa
     2641 tttgtttatc tttgatttta tatgaagtta agccaatttt acctccgcac tttgcacatt
     2701 gtttactcac attagttcct cctagatatc agctttctac tactattaac attctatcaa
     2761 tagtgaccat actttgacca tactttttag aaagaataga aaattccaag atatttaata
     2821 atagaagaaa agcactaaca tcatgggttc agaagaattg agagcttatc aggaaacagt
     2881 ataattgaag tgtctgttta tccagtagct caagaagctt aattgaatct tatgattaaa
     2941 gaggttgtga caagactttt gtcacagcct tttttgttta taaaaataaa agttgtagtg
     3001 gaggctacga agtttacttt gtctttttac acttaatatt tttttgccaa tttggaatca
     3061 ggatgttttg ataactatcg ttttcaattg atggaagcta tattgtttgc tatcagtgaa
     3121 ataattatat ggtaaacagt gttctttttc ttgtttccat tataaaaatc gtatcgggat
     3181 ttttcgttct tttaaattga acatttctat ctatccgcgt gttatcaaca aaacaaaatc
     3241 aggtaaatag ttgacgaaac agaaataaat acatagtatt atgttttgaa gcctaagtac
     3301 aatagtctat agaaaaggag cgataccatt ggcaaaacgc attgaccgaa tttatatgta
     3361 tgtcaaagaa aaaaccgctc atttaacaaa ggcagaatat gatcaaggag taacaacaca
     3421 ggaaattgct gaagtgttag ggattcagcg aacaaatagc agtaaagatc tgaatcagct
     3481 agtgcgcgaa ggaaaactga taaagacaga cggacgacca gtacgttaca tatatcaaac
     3541 actcgttacg cacgtaaagc cagttacaaa acatgtagtc agttacaaag aaacgccggt
     3601 ctctgaagaa aaaaatacac tccaaataga tactaaggat atctttgcaa aaatcattgg
     3661 agctaacggc agcatgaaaa attcagtgga acaggcaaaa gctgcgattt tgtatccgcc
     3721 aagaggatta aattgtttga ttacaggacc taccggatca ggaaagactt attttgcaca
     3781 cgcgatgtat cattttgcaa aagcgcatca tgtaatagat gaggaaacgg aactgattgt
     3841 tttcaattgt gctgattatg ccaataaccc tgagcttttg atgagtcatt tatttgggta
     3901 cgcaaaaggt gcttttacag gcgcagatga agaaaaaatg gggattatcg atcaagcgga
     3961 tggcggtatg ctgtttttgg atgagattca ccggttgccg cctgaaggtc aagaaatgat
     4021 cttttacttt atggatcatg gaacttacag tcgtcttggg gaaacaacga aatcccatga
     4081 agcaaatgta cggatcgtag gtgcgaccac agaagaccca ggttcttctc tattagaaac
     4141 ttttgttcgg cgtatcccaa tcaatatcaa acttccggct tttgaaaaac gtcctgcaaa
     4201 tgagaaagtc gatttagtaa aaatcatgat tgcccacgaa gcaaaccgca cgcagcgaaa
     4261 gatttctttg acagaagatg tagcaaaagc attgatcggc agtgtgacct atggaaatat
     4321 aggacaattg aagtcaaata tccagttagt atgtgcacga ggatttctaa atcatatgca
     4381 atcacccgaa atctcgatca caatcgatga tttgacagaa gggattcgta gcggattgat
     4441 tcaattagca agcaaccgca gtgcaatggc agagctttct aaaatattgg agccaaagat
     4501 aactgtagca ccaaatgata cgatcatgaa aatccaatcg gactcctatg agttacctta
     4561 taatctatat gatatcatcg gagataaagc agctttactg aagtcggatg gtttggatca
     4621 agaagcaatc aatcatttca tttctacaga tatcaacatc catctgaagt ctttctataa
     4681 agaccatggt ttttctttta atgcagacag caaattagca gaatttgtag atccgaaaat
     4741 catagaagtg accaatcaga tttatgtaat ggtcaaaaga tctttaccat atgagtttca
     4801 gcaaaacttt atttatgcta tgagcttgca tatcagttct tttttgaaaa aaatcaactt
     4861 aggagaagaa cggcatacga atgataacat ccgtgagatg gcttttgatt ttccagatga
     4921 atatgctgtt gcgaaagaag tgcgtagtta tatcgaaacg tattttcaag tggagattcc
     4981 agaaagtgaa gactattact tgaccgttct actcgtctcg ttacgggcaa atcaagcaag
     5041 cggcagaatc ggtgtagtcg ttgcagctca cggaaacagc acagcaagca gtatggtcca
     5101 agttgtccaa cagttgttag atgcagagaa cgtacgagca gtagacatgc ctttggatat
     5161 ggatccgaaa acagcattgg accgaattga acgcaatgta caagaagtag atgaaggaag
     5221 cggggtcata ctcttagtag atatggggtc ccttgcgtcg tttaattcgc aaattcaaag
     5281 agacactgga attccagtac gtactgttga tatggtaaca acttctctag ttttggaaac
     5341 tgtacgaaag gtctcagtat tagggacaga tctagatatg ttgcatgaat cattgaaaaa
     5401 ttttcgaggt tacgcagaaa tatcttctgt agaatctgtt caggaagcaa aacctattca
     5461 taagaaaaaa gctgttttgg ctatctgcgc atctggcgaa ggtacagcac aaagaatcaa
     5521 agaattgatc gaacgagcgg taaacaagcg tcaagaaaca gaattagaag ttctagcttt
     5581 gtcgattgtg gaagtgaaag aagaactgcc agttatccaa aaagaatatc agatcatagc
     5641 tactactggg attactgatt tgaatatcca agccccattt attcctttag aacgttttat
     5701 tgatcaaaat atcgaagtca ttttggatca gttgctgatg gaatcagaat tggaggaaac
     5761 tgaattcatc tccctagatg aagagtcagc taaaaatacg tgtgttgagt ttatctctga
     5821 taacttcatc tttattaatg gtagtaagtt aatcgatcca atgtggcaat tttctacaca
     5881 aatctctcag acgacgggaa tcggcgatga agaatatgga ttcaaaatca acctagtgat
     5941 gcacacagca ggaatgatcg aacgtattat tcggaatgaa ccattgactg tggaagaaaa
     6001 cgaattgaca aatacaacga atgatcctct ttatagccaa ttggcagcct cagttgttct
     6061 gttggaagat cagataaaag taaaagtgcc gatagaagaa atgtattatc tgctacgtct
     6121 agtccataat cagttagata aaaaagaata cactgtgcca tgaatacaca aaaacgatac
     6181 acttttatag tgtatcgttt ttgtgtattg gttaagaatc ctttaaattc aattagtgta
     6241 tcgtaaaaaa gttggcacgc tttttgctta tagtagtgtg tgctaggagg tggttgttca
     6301 cacagaaatg ataggggttt ctgtgaagta gtgaggaaga aggagttttg cagatggata
     6361 ttcgattggc acgtatagat gatcgtttaa tccacgggca agtcgcaaca gtttggtcga
     6421 agcaaacagg tgttgaacgt atcattgtga tcagcgatgt agtcgctcaa gataaattga
     6481 gaaagtttct actaaaagaa gctgcaccgc cggggatcac agcaaatgta ataaccgtgc
     6541 agaaaatgat tcaaatctat catagtggat tattatcacg agcaaaagtg atgcttttat
     6601 tcaccaatcc ccaagatgtt gaagcggttg tccgctgcgg tgtacagttg aagtcgttga
     6661 atatcggcgg tatgagtttt actgaaggca aatcaatgat taccaatttt gtttcagtaa
     6721 atcaacaaga tattgattcc tttcgttact tgaacagtca agaagtggag ttagagattc
     6781 gaaaagtacc tgctgacagg aaaatccttt tgatggaact actagcgaaa atcaaaaagg
     6841 tgtagcaaga atggttgttg gaaaaggtca aataattctg ttgtaatggt tagcaattat
     6901 taatattttt gaatttgtta caacaaaagt aaataggagg tataaatatg gtagggatta
     6961 tcctagcaag tcatggacaa tttgctgaag gaatcttaca atccggttca atgatctttg
     7021 gcgaacaaga aaacgtcaaa gccgtgattt tgaaaccaag tgaaggtcca gatgacttga
     7081 gagccaaatt ggaagaagcg gttgcttctt ttgataatca agacgaagtg ttattcttag
     7141 ttgatttatg gggcggaaca ccgttcaatc aatcaaacac attattcgaa gagcacaaag
     7201 ataaatgggc aatcgtcagc ggtttgaatt taccaatgtt gattgaagcc tacgcatcac
     7261 gcttttcaat ggaatctgct catgaaattg cagcacacat cattgaaaca gcaaaagacg
     7321 gtgtaaaagt aaaaccggaa gaattagaac cggcagaagc accgaaagca gcagttgaag
     7381 acgcacaacc taaaggagca cttccagaag gaactgtggt cggagacgga aaaatcaaat
     7441 atgtattagc acgtgttgac tcacgtctgt tacatggaca agttgcgaca gcttggacaa
     7501 aagcagtgca accaaaccgt attatcgttg tttcagatgc agtatcaaag gatgatctgc
     7561 gtaagagact aatcgaacaa gctgctcctc caggtgtaaa agcaaatgtt attcctatta
     7621 gcaaaatgat cgaagtagca aaagaccctc gttttggaaa taccaaagca ttgcttttat
     7681 ttgaaaatcc tgaagacgta ttgacagctg ttgaaggtgg cgtagacatc aaagaattaa
     7741 atgttgggtc aatggcgcat tcggtcggta aagtcgttgt aagtaaagta ttgtctatgg
     7801 gacaagaaga cgttgaagcg tttgagaagc ttgaacaaaa aggtgtgaaa ttcgatgtac
     7861 gtaaagtacc taatgattca cgcgataaca tggatgatat tttgaaaaaa gcaaaagctg
     7921 aattagcaaa agcgtaataa ataatattaa aatttatagg aggcttatca tgtctattat
     7981 ttcaataatt ttagtcgtat tagttgcctt tctagctggt atggaaggta ttcttgatga
     8041 atttcaattt catcaaccac tagtggcatg tacattgatc ggtttagtta ccggtaacct
     8101 cgaagctggt atcgtacttg gcggtacgct tcagatgatc gctttaggtt gggcaaatat
     8161 cggtgccgct gtagcaccag atgctgcatt agcatcagta gcatcagcaa ttattttagt
     8221 tttaggtgga caaggtgtta aaggtgttcc ttctgcgatc gcgattgccg ttcctttggc
     8281 tgttgcaggg ttgttcttaa caatgatcgt acgtacaatc gctgttccaa tcgttcattt
     8341 gatggatgcg gctgctgaag aaggaaacat ccgtaaggtt gaaatgtggc acatcattgc
     8401 tgtatgttta caaggggttc gtatcgcggt cccagcagca gcgttgctat ttatcccagc
     8461 acaaactgtg caatcattcc ttgaatcaat gccagcatgg ttaacagatg gtatggctat
     8521 cggtggggga atggtcgtag ctgtaggtta cgctttggta atcaacatga tggctacaaa
     8581 agaagtatgg ccattcttcg ttattggttt cgttgtagct gcaatttctc aattaacatt
     8641 gattgctcta ggtgctttag gtgttgcatt agctcttatt tacttgaacc tttctaaaat
     8701 gggtggttca tcaaataatg gaggcggaag caactctggt gaccctttag gcgacatttt
     8761 gaatgactat taatcttgaa ggaggaaaat aaaatggcag aagaaaaaat caaattatca
     8821 aaaaaagatc gtttagctgt tgcgtggcgc tctaccttca ttcaaggttc atggaactac
     8881 gaacgtatgc aaaacggtgg ctgggcattt tctatgattc cagcaatcaa aaaattatac
     8941 aaaacaaaag aagaacgttc tgcagcatta aaacgtcact tggaattctt taatacacac
     9001 ccatacattg cttctccaat tttaggggtt acattggctc tggaagaaga acgtgcgaat
     9061 ggcgcgcctg ttgatgatgt agcgattcaa ggggttaaag taggtatgat gggtccttta
     9121 gctggtgttg gtgacccagt gttctggttt acagtacgtc caatgctagg tgcactaggt
     9181 gcttcattag ctatgggagg aaatatcctt ggaccaatca tcttcttctt aggttggaac
     9241 attattcgtt gggcattcat gtggtataca caagaatttg gctacaaagc tggttctaaa
     9301 attactgatg acctttctgg cggattgtta caagacgtaa caaaaggtgc ttcaatttta
     9361 gggatgttcg tattagctgc ccttgtacaa agatgggtat caatcaaatt cttaccagtt
     9421 gtatcaactg taaaattaga taaaggtgct tacatcgaat gggataaatt gccttcaggt
     9481 ggtgaaggaa tcaagagtgc atttgaacaa gttaataatg gcttagccct ttctcctgaa
     9541 aaggtaacaa cattacaaga caacttagac caattgattc ctggtttagc tgcattagga
     9601 ttaacattgt tatgtatgtg gttgcttaag aaaaaggttt ctccaatcgt tatcatcctt
     9661 ggtttattcg tagtcggtgt tgttggacac gtcatcggac ttttataatc cataaaaata
     9721 tagctcgggc gttgtcccga gcttttttta tatagtatac tgttcataaa caaatatctg
     9781 gagggagaaa atggttcaat caatcaatac aaaagtagac ttggtaattg atgctacttc
     9841 gtttacgggt ctgacagatt acgggcaaat catgataggt gataatggtt ttgagtttta
     9901 ccatgcgcga gatagacgca aatatatcca aattccatgg gaagaagtgg attatgtact
     9961 agcatcagtc atgctaaagg gaaaatggat tcctcgatat gccattcaaa cgaaaaaaaa
    10021 tggtacatac acattttctt ccaaagaacc taaaaaagtg cttcgtgcga tcagagaata
    10081 tgtagatgaa gatagaatgg tacaatcatt gagtttcttt gatgtagtga aacgtggtat
    10141 tcgtagtatg tttaaacgca agtaaaaaat aaagaaaact tctaagagga ggttttcttt
    10201 atttttttac tttgcagaag aaaatatgaa atagcaaaac caattatttg tcccctttaa
    10261 atcccatatg ctaaaattga ttagcaaaga aggaggttat attatgagtt ggttatggac
    10321 attgattgtt ggagccgtga tcggagcaat tgcaggtgcg atcaccagta aaggaaattc
    10381 gatgggctgt gtaacgaata tcattgctgg actagtcggt tcaagtattg gacaagcgat
    10441 tttcggcgaa tggggaccaa aagctgcagg tatggcactt attccatcta ttttaggcgc
    10501 agtgattttg gtagcagttg tctcattttt ctttggaaaa cgatcataag tttttacaaa
    10561 atagtaaact aaaaggagtt gcagcgagac tgtgactcct ttttggcttt atttatttaa
    10621 aataaaaaga agttttccgg aaatttttat ttaactgtcg tgtacgaaga aaagtttgct
    10681 ataatggaaa aaagcgtgca aagaaagagg gagtttcatt atgatgctac gtgtagaaaa
    10741 attgcgcaaa aaaatgcaag aagaaaattt ggattcattt ttggttacca gtccgtacaa
    10801 tcttcgttac ttaacgaact ttacaggtac gacaggatta gctgttatta ctttggaaaa
    10861 agcatttttt attactgatt ttcgttatac cgagcaggcg gcagcgcagg ctcaagggtt
    10921 tgaaattatt aaaaatgtcg gacctatttt tgatgaggta gcaaacttag tacgcaaaga
    10981 agaactgcac tcattagctt ttgaggaaac aacagtctca tttttagaat attcggtttt
    11041 agaagaaatc atcgatgcgg aattagtacc tgtttcacag atgatcgaag aactcagaga
    11101 agtgaaagat gaagaagaaa tcgctattat cgaaaaagca tgtcatattg cggatatggc
    11161 atatgatcac atcctgaaaa tgatccaacc gggcatgact gagattgaag tcgcaaatca
    11221 actagacttt tatatgcgtt ctcttggcgc ttcaggtgtt tcttttgata cgatcgttgc
    11281 tagcggattg cgttcggcta tgccgcatgg tgtagcaagc aaaaaaatca tcgaacaagg
    11341 tgacttgatc acattggact ttggttgcta ttacgaaggg tacgtttccg atatgacacg
    11401 tacattcgct attggtgatc ctggggaaaa attaaaagaa atttatcaaa tagtcttaga
    11461 tgctcaatta gcagttattg atgctgcaaa accaggaatg actggtgtcc aattagatgc
    11521 agttgctaga gattatattt caaaacatgg ttacggggaa gcatttggac attctacagg
    11581 acatgggatc ggtttagaga tccacgaagg accaaacgtt tcttctcgtg cagaaaagca
    11641 atttgtacca ggaaatgtga tcactgatga accagggatc tacttaccag gaatcggtgg
    11701 agtacgtatt gaggatgact tgttgatcac agaagatgga aatcgtgtgt tgactcgttc
    11761 accaaaagaa ttgatcatct tatagtttca tcaatagaag aagtaagcgt aaaaatcagt
    11821 gttcacagac tttgttatag aaaatcactc gatttaatga tacactatag aagaactttt
    11881 aagctgtaag agataataaa gttgggtgtt ttctccaatt catagcagtt tgcagctgat
    11941 cggcggcagt tttcttacca cgtgatcact atgccgcatc atagagaatt cggatgtatc
    12001 tcgtgcttag gcaaagggag gaataaataa atggcagatg aaaaaaattt gattttaaac
    12061 gcaaaccaag atttaggaga gatcgtgatc gctcctgaag tcatcgaagt aattatcggg
    12121 attgcagctt ctaaagtaga aggcgtatac ggaatgcgcg gaacttttgc cagcaatgtc
    12181 actgaattat taggacgtgc agctcacgga aaaggtgttt acttagcaaa cgaggaagat
    12241 ggcttgaaag tagatcttta ctgttatcta gaatatggtg tttcagttcc taaagtagca
    12301 atggaaatgc aggaacgggt cagccagcaa gtccttttca tgacggatat cgatttatct
    12361 gaagtcaata tccatgtagt agctgttgtt cctgaaaaat tagaacaacc aacaatcgaa
    12421 gaactttttg aagacgaaga ggaagagaat gaataaagaa ttatcaagac acgagattcg
    12481 ggaaatggca ttgcaggctt tatttccatt agacttcaat gcagacctaa caaaagaaga
    12541 tgcgattttc aacgcaatcg aattagacca ccgcgacatg atcaacgaag atgaatcaga
    12601 gtttgttcct gtttatcttg atacgctagt tggcggtgtc tgtgcaaaaa aagatgaatt
    12661 ggataaagtg attgaaaaac atttaaaaaa caattggcac atcaatcgga tctcaaaaat
    12721 ggatttggtg atcttgcgta tcgcgatttt tgaaatgact tatgtgacag atgtaccagc
    12781 ggctgttgct ttaaatgaag caatcgagtt ggcaaaaaca tttagtgatg accgttcacg
    12841 taaattcgtc aacggtgttt tatccaatgt attgaaagaa ttagaagctg gggcgtaagt
    12901 cctagctttt ttctatgaat catcataaac ctgttataaa tcgggaaaat tctatggtag
    12961 aatgaaaaca caaaaaatat ctgggagtgg ttagatggca gaattgatta acggaaaaga
    13021 attagctgaa aaaatgcagg cagagattgc tgaaaaaatc caaaaattga aagataatgg
    13081 tatccatccc ggattagtcg ttttattggt aggggaaaat ccagccagcc aaatctatgt
    13141 aagaaataaa gaacgtgtag caaaagaaat cggaatccat tcattggtag agcgttatcc
    13201 tgagacgatt tctgaagacg aactacttgc agaaattgaa aaatacaatc aagatcctag
    13261 ttttcatggt atattggtcc aattaccatt gccaaagcat atagatgaag aaaagatcct
    13321 gttagctatc cgccctgaaa aagacgtaga cggttttcat ccgatgaatt taggcagact
    13381 ccttgcgggt aaaccggaca agattccttg tactccatac ggcatcatga aaatgtttga
    13441 agcttattct atcgatccgg cgggtaaacg agcagtagtt atcggacgaa gcaatatcgt
    13501 cggtaaaccg atggctcagc tactcttgat ggcagatgca actgttactg ttgctcattc
    13561 taaaaccgaa aatctggctg aactggcaca agaagcagat attttagtcg tagcaatagg
    13621 acgtggacat tttgtcacaa aagaattcgt aaaaccaggt gctgtagtga tagatgtagg
    13681 gatgaatcgt gatgctaacg gtaagctgat cggtgatgtg aaatttgatg aagtggagcc
    13741 tattgcaggc tggatcactc ctgtgccaaa gggtgtaggg ccaatgacta tcacgatgct
    13801 gatgtatcaa accgtaaaat ctgccgaagc ttttggagca ggtgaataga tggcacaaga
    13861 atatttaact gtcactacat taacgaagta tttgaaaaga aaatttgatg ccgatcctta
    13921 tttagaacgc gtatatttaa caggcgaaat atcgaatttc cgtttacgtg ccaatgccca
    13981 tcaatatttc agcttaaaag atgaccatgc aaaaatatct gctattatgt tcaaaggagc
    14041 attccaaaaa ttacgattcc agccaaaaga aggaatgaaa gtccttgtcg ttgggcgtat
    14101 ttcattatat gaagcgggag gttcttatca aatctatgta gagcacatgg agcctgatgg
    14161 aataggtgct ttatatcaag aattggaaga acgaaaagaa aagctgatga aggaaggcct
    14221 ttttcaaggg cctaaaaaaa tgctgccacg tttcccaaag cgaatcgcgg tccttaccag
    14281 tccaagcgga gcggttatcc gcgatatcat cacgacggtg aaaagaagat atccaattgt
    14341 ccaattggtt ttgttcccaa cagttgtaca aggaaaccaa gcagcggaca atgttgtcag
    14401 gaatatccag agagtagaag agatgggcaa tttcgataca atgatcatcg gtcgtggggg
    14461 tggttcgatt gaggatttat ggccgtttaa tgaagagcgg gttgctcggg cgatttatca
    14521 agctaatacg ccggtgattt catctgtagg acatgaaaca gataccacga tcgctgattt
    14581 agtagcagat gttcgggctg ccactccaac agcagctgca gaattagctg ttcctgtttt
    14641 gtcagaagag ttgatgcgca ttcaagaacg gcaagctaga ttgcagcagg cgtttgtacg
    14701 ccagatccag cgcaaacaag aacggttcga gcgtgctcga cgttcctatg tatttcggca
    14761 gcctgaaaga ttgtatgaag gtcagaccat caaattagac cagttgaaac agcgattata
    14821 tcaagctaca cagcaaattt atcatgcgaa agaaaaacaa acgatcgcat taagccacca
    14881 attggaacaa gtggctccgc tttatcgagt taaagcagct cgccaagaaa ctgcttatct
    14941 agaaaaacgc ttagaagaga aaatccaaca atatatgaaa caacagcagc agcgttttgc
    15001 tcaagctgtt caatctcttg atttgttgag tccattgaaa atcatgggaa gaggctacag
    15061 ctatacgacg ttaaatgatc aagtagtaaa aagtgtcacc gagattcatc aaggggattt
    15121 gctgacgatc cattatcaag atggaatcgt taaaggacaa gtacaagaga tagacaaagg
    15181 agaataaaaa tatggaaaat ccaacttttg aagagtcact gcaagaatta gaaaaaattg
    15241 tcatgcagtt agaacaagga gacgttccat tagaatcagc tctagattcg tttaaacgag
    15301 gaatggaatt aagcaaacat tgccaagata cattagctaa agcagaagag accttgacga
    15361 aaatgatgac agaatcaaat gaagaagttg attttgacgg aaacgaggaa agctgagatg
    15421 agattaactg acttttcagc tattcatcta cctgctgtag aaaaagaaat cttgtctttt
    15481 ttagatgagc atacaacgga tagaggactc tatgatgcga tgagttattc agtgaaagca
    15541 ggcgggaaaa gaatacgtcc cttattactt ttaacggccg ttgcttcctt tgatgagcct
    15601 attgatgttc ccgtctatca agtagcagct gctttagaaa tggtgcatac gtattccttg
    15661 atccacgatg atttgcctgc gatggacaat gatgatcttc gtagaggaaa acctaccaat
    15721 cataaagtat tcggcgaagc attagctata ttggccggag atggtttgct tacaggagca
    15781 ttccagctaa tcagtatggc acacttaggt aattctccta agcttctgct gcttcagcaa
    15841 ttagctgttt gtgcgggatc acaaggtatg gttgctgggc aagctgcaga tatcgagggc
    15901 gaatcgaaaa agctttcttt agaagaatta gcatttattc atgaaagaaa gacagggcat
    15961 ttgattcgtt atgccttgct ggctggtggc atattagcga agcaacctga agaaatctta
    16021 ttgctacttc aacgattagc cgaacattta ggtttagctt ttcagataag agatgatcta
    16081 ctcgatgtga ttggtacaac gaaaacatta gggaaaacag ctggtaaaga tgaacgtatg
    16141 gaaaaaaata cttacccgcg tttattaggc ctagaaaaga cacgtgaggc gttggagatt
    16201 gagctgttat ccgccaacaa gatcatagac aaattagaag aggacgtttt ttcttttgat
    16261 ggcgggctga tgcgccagat gatcaagcaa tttgatgtgg agaaatgagg gaaagaatgg
    16321 aaaaagaacg cgtagatgta ttagcggtca aacaaggatt atttgaaaca agagaacaag
    16381 ccaaacgttc agtgatggct ggtttgattt acaacgaaaa aaacgagcgg tttgataaac
    16441 cgggagaaaa aattccagtg agtagtgaat taagagtcaa aggtaaaaaa cttccttatg
    16501 tttctcgagg gggattaaaa ttagaaaaag cactcaaagc atttgatcta tctgttgaag
    16561 gaaaaacatt gttagatatt ggtgcatcca ctggcggatt tactgatgca gctttgcaaa
    16621 atggcgcaaa aatgagttat gctttagacg ttggctacaa tcagctagct tggaaaataa
    16681 gacaagatcc tagagtcgtc gtaatggaaa gagtgaattt tcgttatgca aaacccgaag
    16741 actttacctt aggaattcca gaagttgctg ttatcgatgt ttcttttatt tcactaaaac
    16801 tgatgctgcc tccattacat gctattttaa aagaagaggg agaagtgatt gctttaatca
    16861 aaccccaatt cgaagcaggc agagaagctg tagggaaaaa cggaattgtt cgtgaccctc
    16921 agacccatca aaaagtttta gaagacattt tgtcatttgc ggcacaaggt ggatatgatg
    16981 tgctggaatt aagttattct ccgatcacgg ggggagaagg aaatatcgag ttcttggctc
    17041 acttgaggaa agtacccgaa agcggaacaa tcaattcagc tattaatatg gcagaagtgg
    17101 tttcaaatgc acacgaacag tttgaccata aataaattgt aactgtgaaa atcaagtgag
    17161 aagtatgcgg attcttcttt attataccaa ggaaataagc tatcattgat taagaagaat
    17221 atgctaagga gcgaagacgt aatgagaaaa aaagatcggc atcgtttgat tactcgattg
    17281 ttgagtgagc aagatatccg aaagcaggaa gaatttgttg aaatcctgaa aagcaaagga
    17341 atatctgtta ctcaagctac gatttccaga gatataaagg aactgaaact gatcaaggta
    17401 ccggcttcag atggcggcta ccgttatagc ataccagctg aaacaaacga agatgtaaat
    17461 actaaattgg aaaaactatt aaaagacgct ttcgtggcag ttgaccaaat ggaaaaattt
    17521 gttattttga aaacattgcc gggaaatgct tcggcagcag caaatttgat tgataaacgt
    17581 tacaagaaag aattattttc gattatcaat gatgacgata atgttttgat gattacacga
    17641 actgaagacc aggcagtttt attgaagaaa gacttcctgc attaccttta agttgaggag
    17701 cgaatgtgat gctacaagaa atcagtatta ctaactttgc gattattccg gaattgcggc
    17761 tttcttttca cgaaggaatg accgcgctga ccggagagac tggtgcgggt aaatccatta
    17821 ttatcgatgc tttaggtctg ttagcaggag gaagaggttc aagcgattat atacgtcaag
    17881 gagcagaaaa atgtgtgctt gaagggttgt ttgaactacc aaaacaagaa ggattctctg
    17941 aactgatggt tgaattaggg atcgaaacag atgaggacaa tctgattgtt cgacgggata
    18001 tgtccttaac tggaaaaaat gtttgtcgag tgaatggtca tattatcaca ttagctaatc
    18061 tgagaagaat cggcagttat ttagtagata tccaaggaca aaacgaacat caggaactgc
    18121 tccagcctga atcacatctt gctttattgg atcgttttgg agatgtagct ttccaacaga
    18181 agaaaaagag ttatcagcaa gaatatatct cttatcgtga gctggaaaaa agggtacgaa
    18241 aagtgcagca aaatgaaaaa tcctatgttc agagaataga tatgctccat ttccagcaag
    18301 aagaaatagc tgctgctgat ttgcaagtcg gagaagaaga gaaattaaaa gaagaaagag
    18361 aaaaacttag caattatcaa aaaattgctg atggattagc tgcaggttac ggagcactga
    18421 cgaataatga acaaaacagt gtagatggtg ttggcttggc cgtttcagaa ctgcaaggga
    18481 tcgcccatct agatgtggag tatgaagcaa tttatgaaaa tatccaaagt gcttactatt
    18541 tgctgcaaga tgcaatcggc gatatgagtc gtcagattga tttattggag ttagatgaga
    18601 gtcgtttaga agaagttacg caacgtttag aattaatccg acagttgaaa cgtaaatacg
    18661 gcgaatccat tgagtcaata ttggcttact atgacgaaat caccgaagag ctagcttctt
    18721 ctgatttttc agaaggtcaa ttggacaaaa tgaaagagga actagagcaa aaagagcttc
    18781 tacttcagca acaagccgca gatcttcatg aagcgcgtaa aaaaatcgca aaagaattgg
    18841 aaaaatccat tctgcatgaa ttgaaaagtc tgtatatgga aaacactgag tttgaggtac
    18901 gtttcttaaa agaagaaaat agacaattga atcatgatgg atttgaccaa atcgaatttt
    18961 acattacgac caatccagga gagccactga agcctttggt gaaagtcgct tctggtggcg
    19021 aactgtctcg gatgttgctt gctttgaaaa ctattttttc ttctgagcaa ggtgtgacaa
    19081 gtattatttt tgatgaagtg gatacaggtg tgagcggaag agttgcacag gccatcgcag
    19141 ataaaatttc tcaaatatcg aaatactctc aagttttgtg catcactcat ctgcctcaag
    19201 ttgctgcagt cgccgactat caatattata ttgtgaaagc agtgattgat ggacgtacgc
    19261 aaacttctgt ttcagagttg aaaacaaaag aaagagaaga agaaattgca cgtatgctgg
    19321 ctggaagtga gattacaaaa ctcacactcg aacatgcaaa agaactattg aaactcgcaa
    19381 aaaggacctg agccacctcg cagtggttca ggtccttttc agcagctgat agctatattt
    19441 tatttttaca tgacactcat tacagcagtc aatacaacag atccaatcgt taaaatcagc
    19501 attgtccaaa tgacaaattt tgttacttta gcaaaagtac ttgtttgttt tttttcacgc
    19561 attgttttca acctcgtcat taattagttg attcaagtgt acaatctttt ttccatttcc
    19621 acaagagggc ttttttataa aaacttccct ttttttaaat aataggtagt tacgatacaa
    19681 ataaataatg ttaatagaac tagccatata aaggcatcat ccctttcagc aataggtaac
    19741 ttaatgttca ttccatagat actgccgacg attgtcggta tagttaaaat aatcgttaaa
    19801 gaagttaaaa ttttcataat attattcaga ttattcgata cgattgctga aaaagtctga
    19861 ttcatttgtt ctactagttt taattgaatg cgagaagtag tcattgcctg ctctgtttcc
    19921 accaatacat cacgtaaatg atttttcaaa gcatgatttt cttggaaaag tttattttgc
    19981 gctagttctt tcaatgcatc taaattagca gaagtcgctt ctctgaaata aacgaggctt
    20041 ttttggatat ccatgatttg ataaagttgt ttgttttcag tggatacttg aagtttggct
    20101 tccaaagtat cgcattgaga ttgaagcgta gctaaataac tgttgtaaga gagtgtgacc
    20161 tgccaaatga attgaaagaa gatattgaga ttaggcgaaa tatcactttt tggaaaggtc
    20221 tggctaaaga ctttctttaa aaacagtggt tcatgattcg taattgtgat aagcttcttt
    20281 ttgggcgtca aaataataga gagcgggtat gtatccactt gaaaataacc gctaggactt
    20341 gttttagcaa aaggatattg taacaataat aacgctggct ttgtcaactt tgattgattg
    20401 aattcttctg tacgggaatt ttctgcatcg tccaatatgg aagtgatgta atctgacgga
    20461 atttcaaatt gatgtgtcag ctgatgaatc tcttcttgat taggacgttc tacacaatac
    20521 cagatcgttt ggtcagtttc ctttccggta ttacgcagtt tctcattatc taatgtaaaa
    20581 tatttgatca tgattttcct cctgttgcct gtttctttta ttataacgga tagggaattg
    20641 gaatacatgg aatgtgtttg taagatatag aatataactg cataaattta aataaaatga
    20701 aacagttgtg taaagtatat acagtatact tgaagcaacg ataaaaaagg agtttacgtt
    20761 catgggcaca ttcacaccac ttatcttgtt ttggtatatt tacccggtaa tcgttttgtt
    20821 tggttgccaa tttcttgtaa aattattttc gttaaatcgt cgttttaaaa taaaagcccc
    20881 tgatttggcg attccttttt tgtggggcgg tatgcatgca ttgtctagaa atacagggac
    20941 aatttctatc ttaccgtttt tctttatttc tgttttgcta atgggaattt gtattgctgt
    21001 tttccaagca tattattatg aagaaattat ttatccgcgt tatttcaaga tgacttggcg
    21061 tttggttttt ctgttgacaa tcgtcttgta cgctgtcttg atcattgtga acatactttc
    21121 ttacctatga aaaaaataga aaagctgtaa aaagtctttt aaaaggcttt ttgcagcttg
    21181 atttttaaaa aaatgtttat gaataaaaaa agttgttccc ggatttccca gaaaaggtgg
    21241 tagaaagtgg gggattgtgg tagactaatt ttagttagtg gaggaatggg gggctttcaa
    21301 tgttgatggg tgaattccaa cataatattg atgcaaaagg acgtttaatc gtgccctcaa
    21361 aacttcgtga agaacttggc gaaaagtttg ttctgacacg cggattggat ggctgtcttt
    21421 tcggctatcc gatgagcgaa tgggaaaatc tggaagctaa actgaacgaa atgccgttgg
    21481 ctaaaaaaga cgcacgtact tttgtgcgat tcttctattc ggctgccact gaatgcgaac
    21541 ttgataaaca aggacgaatc aatataccca gcacgttaag aaattatgca gctttgacaa
    21601 aaggttgcgt agtcatcggt gtatctaacc gaattgaaat ttgggatgag acacgatggc
    21661 aagatttttc tgcagcagca gaagagaact ttgacgaaat tgctgaaagt atgattgatt
    21721 tcggactata agaataggaa gaggctgact aatgactgaa acatttcaac actatacagt
    21781 catgttgaaa gaaacggtag atggattgaa catcaaaccg gatggcgttt atgtagactg
    21841 tacactcggc ggtgccggac atagtgaata tttattaagc caactaagtt ctaaagggca
    21901 tctgtatgcg tttgaccaag atcaaaaagc aatcgatcac gccaagctgc gtttagcttc
    21961 ttatgtcgaa caaggtatgg tcacatttat caaagcgaac ttcagagaat tagaagaacg
    22021 cttgagtgag catgtagata aggtagacgg cattttgtat gatctagggg tttcctctcc
    22081 tcagctagat gaagcagaac gtggatttag ttatcatcaa gatgcaccgt tggatatgcg
    22141 catggatcag tcagctcctt tatccgctta tcacgtaatt aatgaatatt cctaccatga
    22201 attagtaaag attttcttcc gctatggcga ggagaaattt tccaaacaaa ttgctcgtga
    22261 aatcgaacga gtacgaaaaa caaaaccaat ccaaacaact ggtgagcttg tcgaaatcat
    22321 taaacaagtc atcccagctc ccgcaagaag aaaaggcggg catcctgcaa aaagaatttt
    22381 tcaagctgtg cggattgcag taaatgacga actcggtgcc gaagaggcct ctttagaaca
    22441 ggcaatccgg ttattgaaag tcaatggaag aatcagcgta atcacattcc attcattgga
    22501 agacagaatt gtcaaaagta tgtttaaaga atatagtacg gtacaagatc taccaccggg
    22561 acttcctgtc atacctgacg aattccaacc agaactgaaa gtcattactc gtaaaccaat
    22621 ccttccttca gaggaagagc taacagaaaa caatcgatca cgcagtgcaa agttacgtat
    22681 tgcagaaaaa ataagaatga aagaggagta gaatgatggc agaattaaag aaaatgcaag
    22741 attacgaata tgatttacct gaattggtgg atcagccgga acaacaaatg cctgattctt
    22801 cttcgaatca agaaatcatt cttccacaat caccggcaaa acgattaaag cagatttctg
    22861 ctttggaaaa aattgctgtg gcaagcgtgg cgattgctat tgttgcttta tgtgttttaa
    22921 cagttatgct gcgcacaaat atcagcggag ctgaaaaaga aatcacgtca atacaaacag
    22981 aaatcaatga taaaaagcaa gaaaaaacga gtttagaaca agaaaaaaac gaactttctc
    23041 gtactgaacg gattaaacaa atcgcagaag aaaaaggatt gtctattaat gacgacaatt
    23101 taaggaaagt gaagtaaatg agtcttaaaa ataaaattcg gcggtttatg gaaaagaaaa
    23161 atctaaatcc gatgaacaat cgcaaaaaag taggtatcat tttatttgct acgagtattg
    23221 gattgttctt tttatttgcc gtccgtttct cgtatatcgt tattggaggt catgttgctg
    23281 gtacctcatt agctgaaaaa acaaaacagt tgtaccaagg aagtgaagta gtaaaagcaa
    23341 aaagaggaac gatttatgat cgtaacggtg tggcgctcgc agaagatgcc tcctcttatt
    23401 caattaaagc gatactctca aaaacatata cctctgggga taaaaaactg tatgttgaag
    23461 aaaagaattt tgataaaata gcagaaatcc tgcataagaa tctttctata gacaaaaaag
    23521 atgcactgaa tattttagag gacggcgcaa aaaaagagct gtatcaagtt gaatttggta
    23581 gctatgggaa aaatatctcc caagaaacca agcaaaacat cgaagcggac atgaaaaaag
    23641 aaggtgtcgc tggtctttat tttgtcgacc atcaggctcg gatgtatcca aatggcgtct
    23701 tttcttctca ttttattgga tacgcagttc cagacaaaga tgagaatggc ttagttggta
    23761 aactaggttt ggagtctgct tacaatgata tcttgagcgg taaagatggt aagattattt
    23821 atcaaaaaga taattttcaa aatcctctgc caggtacagt ggcagaagaa gaaaaagctg
    23881 tcgacggaca agatatctac acgactttag acagcagact acaaagttat ttagaaacac
    23941 tgatggatca agtaaatgaa gaatatcagc cagaagaatt gacagcagtc ctgatgaaag
    24001 caaaaaccgg agaaatcttg gcaatgggac aacgacctac tttcaatcct gaaacaatgg
    24061 aaggattgac tggaaaagat gctatctggc gtaatttctt ggtacaagat agttatgaac
    24121 caggatcaac catgaaagtt ttcactactg cagctgctat cgaagaagga gaattcaacg
    24181 agaatgaaac cttccaatct ggaaaaatcc aagtagaaga tgctacgatc aatgaccatg
    24241 actttggtga aaaaggtgtg ctgaccatgc gtcaagctct ttcatggtca agtaacgtcg
    24301 ggatggtcat actggaacag cgattaggcg gacgatggta taactattta caaaaactgg
    24361 gatttggaca aagtactcat tctggattag atgatgaagt aaacggtgct ttacctacat
    24421 taaatattgt cgaccgagca atgagtgctt atggacaagc ggtaggtgtg acgaatttcc
    24481 aaatgatgaa agctttcact tctattgcga ataacggtac aatgattcaa cctcgatata
    24541 tcagcaaagt ggtggatccc caaactggag aagagcgaac aactcaaaca gaagttctag
    24601 ggcaaccttt ttcaaaagaa acaacagaaa aagtccgtga atatatgaga gatgttgtcg
    24661 aaagcgaaaa ctacggtagt gcttatggtg tatatagtgt acctggatac aatgtatcgg
    24721 caaaaagcgg aacagcccaa atcgcttctg atacaggggg atatcaaaca ggagatacag
    24781 cctacctgta ttcgattgtg gagatggttc cttctgaaga tccagactat gtcttgtatc
    24841 taacaatgaa acaccctaag acatatgatc gaatggcatt ggctaaaatc gcaaatccat
    24901 tgatgaaacg ggcaatggat tttaaagaaa ctgaagaaga ttcagataca gaaacaaaaa
    24961 cagaaaaagt atccgtagcc gattatcgta atcttgaagc ggacgttgca gcagctgatg
    25021 ctcaaaaaag tggactgcaa cctgtagtga tcggtaatgg taaaaaagta caaaaacaat
    25081 ctactgccaa cggtgatcag ttgatctctg gagaaaaact gatcctatat actggtggtg
    25141 ataagctgat gcctgacgta acaggatggt caaaagcaga tatcatgaag ctggggaaaa
    25201 tcctcggtat tgaagttagt tttgatggag atggttattg tgtcaaacaa gaacttgctc
    25261 catatgaaaa aatcactgaa gacaaactta gttttacttt agaagaataa gaagagaaat
    25321 aggagagaaa agagcatgga atggacacaa gcattgattc ccattgtcag cagttgtgca
    25381 atgacaattg ccgcaatgcc gctatttatt ggttattttc aaatgaaaaa gcaaggtcag
    25441 gcgattcgtg aggagggacc aaagtggcac aatagcaaag ccggaactcc tacaatgggc
    25501 ggcttagtgt ttctgatcgg ctctattttg acaggaattt gggttggagc ttggcaaaaa
    25561 cagctaactc ctactctttt catcttgctt tttgtattag cgttatacgg agtaatcggc
    25621 tttcttgatg attttataaa aatattcaag aaaagaaaca tgggactgaa ctcgaagcag
    25681 aaattgattg gacagattct agggggaatc atcttttatc ttgtttaccg ttctgaaggt
    25741 tatccaggtg tattgaattt cttcggacta gatctacctt taggaatcat ttatggtatt
    25801 ttcgctctct tctggcttgt tggattttca aatgcagtaa atctgacaga tggcatcgat
    25861 ggattagttg ctggcttagg aagtatctct tttgcagctt acggtctaat tgcttggcat
    25921 cagcatcagt atgatgtatt aatcatttgc ttaagtgttt taggaggttt actgggtttc
    25981 ttcgtctaca acagaaaacc tgccaagatt tttatgggag atgtcggttc attagcgttg
    26041 ggcggacttt tagcggcaat ttctataatg ctaaaccaag aatggacatt gcttttgatt
    26101 ggtttgatct atgttatgga aacagcaagt gtgatgcttc aagttacttc ctttaagctt
    26161 acaggaaaac gaatttttaa aatgtctcca attcatcatc actttgaaat gtgcggatgg
    26221 tcggagtgga agattgatac agttttttgg ttgatcagca ttgtgacttc gttgatcaca
    26281 ttatggatcg tttggtagga gatgacggga aatgaaaaaa atatcaacat atgaaaataa
    26341 gaaagtgctt gtcttaggac tcgcaaaaag cggagtaagt gcggccaaat tattacatga
    26401 attgggcgca cttgtcaccg ttaatgatgg aaaacctttt gacgaaaatc cggaagcaca
    26461 agagctgctt tctttaggga taaaagtaat aacaggtagt catccaatcg aattgttgga
    26521 tgaagaattt tcattgatgg tcaaaaatcc agggattccc tacagtcatc ctttagtagc
    26581 aaaagcccaa gaaatgggta ttccggtaat tacggaagtt gagctagctt acgaagtagc
    26641 ggaatgccca attattggga ttacaggaac gaacggaaaa acaacgacaa caacgatgac
    26701 aggactacta ctgaatgcag gagcagatca aggaattgca cgtttagcgg gtaatattgg
    26761 gtatccagca agtggtgtag cgcaagaagc aaaatcagaa gataaaattg tgatggaatt
    26821 gtctagcttc caactcatgg gaatcacaga tttccgccct catatagcgg tgattaccaa
    26881 tatctatgaa gcacatattg attatcatgg tacaagaaaa gaatatgtca aagctaaatg
    26941 gaaccttcaa aaaaacatga cagaaaaaga ttatctgatc cttaactgga atcagtcaga
    27001 actacaagaa ttagcgcaaa gaacaaaagc acgcgtactg cctttctcga caaaagaagt
    27061 gttggaagac ggtgtgtatg ctgacgatta ctcaatatac tataaaaaag aaaaaataat
    27121 ggaaatatct gaacttggcg taccaggtaa gcataatgta gaaaatgcac tagctgctat
    27181 ttctgtagca aaattgtatg gtatatcaaa tgaagctata cgagaaacgt tgcatttttt
    27241 ccatggcgta ccacatcgta cgcaatatgt aggagaaatc caaggcagaa aattttataa
    27301 tgattcaaaa gcaaccaata ttttagcgac taaaatggct ctaagtggat ttgaaacgtc
    27361 taaggtcgtg ttattggctg gcggattaga cagaggaaat acatttgatg aattaattcc
    27421 ttcattaaaa ggaatcaaag caatggtcgt atttggccaa acaaaagaaa agttgatgga
    27481 tgctggaaaa aaagcaggca tcgagacaat agtaacagcc gattctgtag aacaagcagt
    27541 tcctttagca ttagaaaaca gtacagatgg tgatgtagtt ttattatcac cagcgaatgc
    27601 cagctgggat caatacccaa attttgaaac tagaggaaac agattcatgg aagctgtgaa
    27661 ccgattaaaa tagaaaaagt agaagcgagc gataaaaaat gaaaattttg gttacaggcg
    27721 gaggaacagg gggacatatt tatccagctc ttgcctttgt caattatgta aagactcaag
    27781 aaccgaatgc tgaattcatg tatgtgggag caaaacgagg tctagaaaac aaaatcgtcc
    27841 ctgatacagg aatgcctttc cataccttgg agattcaagg atttaaacga aaaatttcca
    27901 tgcataacat caagacggtc cgattgtttc ttaagagtat ccgtcaagca aaaaaaatcc
    27961 tcagtgattt tcaaccagat atcgttatcg gtactggagg atatgtttcc ggagcggtag
    28021 tgtacgctgc agccaaaatg ggtattccaa cagttatcca tgaacaaaac agtgtaccag
    28081 gtgttaccaa taaattttta agccgatatg tggatcggat cgctctttca tttaaagatg
    28141 cggcttcctt ttttcctgaa aacaaagcag ttcttgtagg taatcctcga gcacaagaag
    28201 tagcagatac taaaaaatca gatattttga aaacatttga tttggatcca gaaaagaaaa
    28261 cagtcttgat ttttggcggt agtcaaggag cacttaaaat caatcaagca gtatcagttt
    28321 ttttaagtga atatattcct tcttcttacc aagttttgta tgcttcagga gatagatatt
    28381 atgaagagat cctaaataaa ataaatcctg aaatacaaaa tattagtgtc cagccttata
    28441 tcaaaaatat ggcagaggtc atggcttcca gtgatctgct ggtggggcgt gctggtgcaa
    28501 cttcgatcgc agaattcaca gctctggggt taccggcagt gttggttccc agcccatatg
    28561 ttacgaatga tcatcagact aaaaatgcaa tgagtcttgt tcacgcagga gcagcaaaaa
    28621 tgattgcaga taatgaacta acaggtgaat cactaagtca gacagtcaac gaaatcatgg
    28681 gtgatgaaga attgcaaaag caaatgtgcc gagcttctaa agaacaagga atccctgatg
    28741 cttcaaaacg tctttatgac ttagtaaaac aaataatcaa aaaataaagg cggtggtctt
    28801 agtgagtaag aaaaaagaac caaaaaaatt gtctcatccg tcttctgatg aaaacaattt
    28861 gactccttgg caaaaggcta accgaaaata tctggcagaa caagagatga aaaaccaaga
    28921 gccaaaaaaa gaagacataa cattagatac acccctttca gattctgaag aagaaaaaac
    28981 ggttcaagag gaacagcaag aagaaagcca gtccgagaaa aaaatcgaat tttttgaaga
    29041 gatcaagcaa aagaaaaacg gtggaccata taacggttct tttttgaacc ggctgcccaa
    29101 tttaaaaagt caacggcaca aagtactatt tcgtcggtta gctttgatca tcactgtatt
    29161 gggtataccg ctgatttttt tgatctatta tgtttctcct tatagcaaat tacaagcagt
    29221 tgcagtatca ggcaataaga cagtcaacag ccaagaaatc atctctgata caaaattgtc
    29281 attaggagaa aatgtttggg gacagtattt tcatcgatct acttatatcg atcgccttaa
    29341 aaaagcacag ccaagaatcg aaacagcaaa tatacatttc aaagggatga atgaattcga
    29401 tttggatgta acagaatata aagagattgc tttgatcgca aaaaacaatc aatactaccc
    29461 agtgattgaa aatggaacag tattagatga aaaagtagca aatcctacaa aaaatctccc
    29521 tatcttggaa gagttcaaag actctgcaaa aatcaaagaa ttaacaaagc aatacaatca
    29581 gttatcatca gaattacaaa aagcaatttc tgaaatcaag tacacgccaa gagcaagcaa
    29641 taaaaatctg atccaattga atatgaatga cggcaatcaa gttattgtaa atatcaataa
    29701 tcttgcgaac caaatgaaat attattcgca agtagccaaa gatatggatg aaaaaggtgt
    29761 gatcgacatg gaagttggta tcttctcgca tccttacggt agcactcaag aaacggaaga
    29821 atcgggccaa tcggaagaaa caactgaaag tgaagaaaat gcggatcaaa caaacgaatc
    29881 aagtgaagag gcagaaacgc aaaaagcaca aacacctgga tcgtctagca caaacggatc
    29941 atctgattca aatgattcaa gtgaaacaag tcaaatagaa gataatccaa gtacaactcc
    30001 gtcaagttta aatgaaaatt gataaaacaa ctttaaaaag taagaatgta ctttctaaaa
    30061 accagtaatt tatggtagaa ttggaagcag tgaatatatc tattaataag tatattttaa
    30121 aacggacgct agttgttcgc ttgtatatgg aatagtaagt aggagggaat cccattcatg
    30181 gcaaaaacag gaatgtatgt aggccttgat attggaacga catctgttaa agttgtcgtg
    30241 gctgaatata tcgacagcca aatgaatatt attggagtag gaaacgcaaa atcagaaggg
    30301 attaaccgag gcattatcgt tgatatcgat aaaacggtcc aagcgataca acgagcagta
    30361 cgacaagcag aagaaaaagc aggtatccag atcaaaggcg taagtgtcgg tttgccagca
    30421 aacatgcttg aagtagaaaa ttgccaggga atgattgcag ttaacggaga ttcaaaggaa
    30481 atcaccgatg aagatgtcag aaatgtggct tcagctgccc ttgttcgttc aatacctcct
    30541 gaacgtcaaa tcgtgtcaat attgccacaa gattttactg tcgatgggtt cgagggaatc
    30601 aaagatcctc gtggaatgat cggcgtaaga ttagaaatgt acggtctgtt gtttacagga
    30661 cctaaaacga tcatccataa catacgtaaa tgcgtggaaa atgccggatt aatcgtcaac
    30721 gaaatggtga ttactccttt agcgctgact gaatcaattc tttctgacgg agaaaaagat
    30781 tttggtacga tcgtcattga tatgggtggc ggccaaacaa caacggctgt tatgcatgat
    30841 aagcaattga agtttaccaa tcttgaccaa gaaggtggcg aatttgtcac taaagatatc
    30901 tctattgtat taaatacgtc atttaacaat gcagaagcgt tgaaaataaa ctacggggat
    30961 gcatacccag aacggacatc accagatgaa gaatttccag tagatgtgat tggtcagtca
    31021 gaaccagtga aagtcgatga acgctacttg tcagaaatta tttcagctcg aatggaacag
    31081 attttcaaca aagcaaaaga tgcattggac caaatcgaag cattagagtt gccaggtggc
    31141 gtcgtattga caggcggagc tgctagtctt cctggtgttg tggacttggc acaagaaatc
    31201 ttcggcgtaa atgtaaaatt gtatgttcca aatcacatgg ggctgagaaa cccggtattt
    31261 acaaatgtaa taagtatcgt ggattattca gcaaacttga gtgaagtgta tcaattggca
    31321 aaaattgctg ttacgggtga aacctcagca gctcgtcaaa tggtagttga acaagaaaca
    31381 acaaacacat atgagagcta tgaagcacct gaagaaacaa tctacgatga gccggaacca
    31441 aaagagtccg gagaaaatgt aaaaaataaa atcaagggtt tctttacaaa tatttttgat
    31501 taattcatcc tgggaggaat aagatatgga attctcaatt gataataata tcaatgacgg
    31561 cgcagtaatc aaagtcatcg gagtcggtgg tggcggtgga aatgctgtaa accgaatgat
    31621 tgaagaaaac gttaaaggtg ttgaattcat tacagccaac acagacgtgc aagcattaaa
    31681 aaattcaaaa gctgaaacag ttatccaatt aggccccaaa tatactcgtg gtttaggtgc
    31741 tggttctcag ccagaagttg gacaaaaagc ggcagaagaa agtgaacaat ccttacgcga
    31801 agctttagac ggtgcagata tgattttcat cactgcagga atgggtggag gaaccggtac
    31861 aggtgctgca ccaatcgttg caggtatcgc gaaagaattg ggcgcattga ctgttggtgt
    31921 cgtgacacgt ccattcactt ttgaaggacc gaaacgtggc cgttttgctg cagaaggtat
    31981 cgctcgtttg aaagaaaatg tggatacatt attaattatc tcaaataacc gtttattaga
    32041 agttgtagat aaaaagacac cgatgcttga agctttccgt gaagcagata atgttttacg
    32101 acaaggtgta caaggtatct ctgatttgat cacagcgcca ggttacgtta accttgactt
    32161 tgctgacgtg aaaacagtga tggaaaacca aggtacagca ttgatgggga tcggtgtggc
    32221 tagtggagaa gatcgcgtag tagaagcaac gaaaaaagca atctcctctc cattgcttga
    32281 aacatctatt gatggagctg aacaagtact tcttaacatc acaggcggtt tggatatgac
    32341 attgttcgaa gcacaagatg cttcagatat cgtagcaaat gcagcaactg gcgatgtaaa
    32401 tatcattcta ggtacatcta tcaatgaaga aatgggcgat gaaatccgtg tgacggtcat
    32461 cgcaacaggt atcgatgaat caaaaaagga acgcaagtct agccgaccag ctagacaagc
    32521 acaaatgcaa tcacctgctc aaaagactgt attagatatg gatcaagcta aaccaacttc
    32581 ttcagaagaa gaaaacagtt ttggcgattg ggatatcaga cgtgaacaaa atgttcgtcc
    32641 tagagttgat gattccaatt ttgacaatat cgaaaaaaaa gaatttgata cattcaaccg
    32701 cgaagaaaca aaatctcatg gcgatgatga attaaatacg ccaccgtttt tccgtcgtaa
    32761 aaaataaagg agaggaagag cacatgattg ctgacaactt gcagaaaatc aagcaagaaa
    32821 ttcaggattc gtgtgctctt gttcatcgtg atccgaaaga agttacatta gtagctgtaa
    32881 caaaatctgt tgaatccgct caagctgaag aattggttag agctggcgtt atgaattgtg
    32941 cagaaaaccg agtagataaa ttattagaaa agaaacaaga tttatttgat tttcctgaaa
    33001 taaagtggca tttaattggt aatttacagc gcagaaaggt aaaattgatt gtaaatgaaa
    33061 ttgattactt tcatgcgttg gacagtttaa gattagcaga agaaatccag aagcggttag
    33121 agaaagaatt atcctgtttt attgaagtaa atgtttctgg tgaggaatca aaacatggaa
    33181 tttcaccaga tgagctttta ccgtttgtgg aatctttagc tgcctgcaca aaaatcaagg
    33241 ttgttgggtt gatgactatg gcgcctaaag aagcttctga tgagcaaatc cataaagtgt
    33301 ttggtacgct gaaagaattg cgtgatcttg ttcaagcaaa aggttatgcc catgcaccat
    33361 gtagtgaatt aagtatgggg atgagtcaag actttcaaat tgcgattgaa gaaggtgcaa
    33421 catttatccg tgtaggaaca gcgctattca aagacgaata aaggaggaac aaaaatgccg
    33481 cttttaaaca gaggagcaat tgctaatttt ttcggattag ctgatgagga agagtacgaa
    33541 taccaagatt atcctgaaac aacaaaaaag gctgtacagc caactaaaaa aaatacaaca
    33601 cagaatcaat caactcattc tgtatctgct gcgaatactc atgaaaaaca aaaaacggcg
    33661 acttcacgtc ctgtaacgca tgaaagtatt cagccggctg aaaagaaaaa agtaattgaa
    33721 ctacatcaat cacctacaaa aactgtagcg aaagaaagaa cagcttcaag aacctctaca
    33781 caacctaaag caaatcttgc tggaaaaatc actgtaatgg aaccaagagc ttattctgaa
    33841 gctatggcga ttgctaaacg aatcattgct ggagaagcaa ccttagtgaa ttttcattta
    33901 gtagaagaaa aacaagcgag aagaatcgtt gattttctaa caggaacagt ttatgcacta
    33961 gatggagata tcaaacgtgt tggagatgag atttttcttt gtacaccgac tgggatggag
    34021 attgacagtt caaccgctca atcttttgca gatgcgaatt tgtttgatct gtaagaggag
    34081 gaactacgat ttatactatt gcactattac ttcatttact tcaacgggca gcctatttct
    34141 atagcattat cttgatggta tatgcattgc tctcttggtt tccaggcggg tatcaatcag
    34201 ggctaggacg attattagct aagatctgcg aaccgtatat cagtttgttc gatcgtctgc
    34261 ctttgaggat tggacccttg gatttcacaa ttttaggggc aatcctgatc ttgaacttag
    34321 ccgtacaagg tttagcacag atcgttatat ggataactgt ccatttagtt tattgattga
    34381 agcaacaaaa tctttattct cagaatagat aggaggggaa aattttggat gcaaatgtat
    34441 accagcactt tcgggcagat gaacgtacat ttatcgattc ggtaggcgat tggattgaac
    34501 aggttcaagg acaatatgca ccctatctga ccgaatttct tgacccaaga caatcctata
    34561 ttttagaaac actgattcga caaagcagtg atttgcgttt catgttttat ggcggatatg
    34621 aacaggcaga aagaaaaaga tgtttgattt tccccgatta ttacgaacct gtagaggaag
    34681 attttgaaac agcattgtat gaaatccatt atcctagcaa atttgccagc ctaagtcatg
    34741 ggaaaatcct tggaacttta gtgggaacgg ggatcaaacg ctcttacttt ggcgatatca
    34801 tctcggacgg tgatcgctgg caagtattta ttgcaaatga ggtggatcat tttgttgctg
    34861 cacaagtaac aaagattgga aaggttgctg tacgcttgga accaatcaaa tacacagaaa
    34921 tgattatgcc gaaagatagc tggacacaag aaagaggaac agttagttcc atgcgactag
    34981 acagtgtgat ctctgagatt tataatattt ccagacagcg gtccaagcag ctgatcgaag
    35041 ctggtaaagt aaaagtgaac tggacagaaa atacaagacc tgattttgta cttgaactat
    35101 tagatattat ttcagtcaga ggttatggca gagtccagat ccaagaactt gaggggaaga
    35161 cgaaaaaaga aaagtacagg gtactgttcg gtgtattacg taaataaaat aaaaaggtgg
    35221 tttactcatg gctttaactc cattagatat ccaaaataag acattcccga caaaaatgcg
    35281 gggatataat caagacgaag tagatgattt cttagattta gttgttcgtg actatgagga
    35341 attgactcaa agaaatcgag aattagaaaa agcagtcaaa cattctgaag aaaaacttga
    35401 gtacttcaat gaattaaaag atgctttgaa ccaatcaatc atcgtagctc aagatacggc
    35461 tgataaagta aaaacaagtg caagcaagga atccgaagtg atcgttactt ctgcacaaaa
    35521 caaagcagat gagttagttg cgaacgctga aaaacgtgca catcaattaa caactgatgc
    35581 agaagaaaaa gcaagaaaaa tcttgacaga tgcaacagaa aaagcaagac agcttgcgac
    35641 agaaactgaa gatcttaaga aaaaaacacg agtattccat caacgtatca gcttgatgct
    35701 tgagtctcaa ttagaacaag tgaaaagtcc ggaatgggat gaaattttac aaccattctc
    35761 cagctacgtg actgacagcc atgaagtgat caaagaagtt ttagcaaaac agcttgacaa
    35821 tgaaaatgat actgatgtaa actcagacac aggagtcata gaagcaccta tgacggcatt
    35881 tgatacacag gctattgata tctcgatcgt gcctggtgaa aatgaaaacg agtagaacag
    35941 aaaagcactt tgttattctt aaagcgagcc gatgagagtg gaagttcggt aagacactgc
    36001 atggtgaaag atcctctact tgagacctgc ttttccgaaa ttagagtaag aagcagcggc
    36061 tgacctcgtt attggtcaca gagagtttgc actagataga tgcaactaaa tttgggtggt
    36121 accacggcat gttcgtccct tgcggctgaa catgcttttt tgtgtccgga aaacaaataa
    36181 aggagtgtat ggttgaatga aaatgaaaga aaccttgcaa ttaggaaaaa caaaattccc
    36241 tatgcgtggt aatttgccaa accgagaagg cgagtggcaa aaagaatggg aagaaaatcg
    36301 aatctaccaa aaaagacaag agttgaacga aggaaaacct agctttgttc ttcatgatgg
    36361 acctccatat gccaatggta atatccattt aggacattca ttgaataaaa tcagtaaaga
    36421 cattatcatt cgttcaaaat caatgtctgg ctatcgtgca ccatatgttc caggatggga
    36481 tacacatgga ttgccgattg aacaagtatt ggctaataaa ggcgtgaaac gtaaagaaat
    36541 gtccatggca gaatacttgg aaaaatgtcg tgaatatgca ctttcccaag tagataagca
    36601 acgagcagat ttcaaacgcc taggagttgc aggagattgg gaacatcctt atgttacatt
    36661 agatccttct tatgaagctg cacaagtacg cgtatttggt aaaatggctg aaaaaggcta
    36721 tatttacaaa ggattgaaac caatttactg gtctccgtca agtgagtctt ctttagcgga
    36781 agctgaaatt gaatataaag atgtaaaatc tccatctatt tttgtagctt tcaatgtcaa
    36841 agacggcaaa gatattcttg atgaagatac agcttttgtc atttggacga caacaccatg
    36901 gacattgcca gcaaaccaag gtattgctgt aaatccaaaa tttacgtatg tattggtgga
    36961 agccgatggc cgtaagtttg tcgtagcgaa agatttgcta gaaacagtcc aatcagaaat
    37021 cggttgggaa aatgtaacag ttctaaaaga atttgctgga caagaaatgg aatatatgac
    37081 tgcaagtcat ccattctatg aacgtgaatc acttgtgatt ttaggcgatc atgttacttt
    37141 agacgctggt actggattag tccatactgc tccaggacat ggggaagatg actatatcgc
    37201 tggtagcaaa tataaactat ctgtagtgtc acctgtagac tcaaaaggat tgtttacaga
    37261 agaagcacct ggatttgaag gtatctttta tgacaaagca aatcctatga tcactgatct
    37321 attaaaagaa aaaggagctt tgctgaagct agatttcttt actcatagtt atcctcacga
    37381 ttggcggacg aaaaagcctg tgatctatcg ggcaactcca caatggtttg catctatcga
    37441 taaattccgt cagaatattt tggatgaagt agaaaaagta gagtgggtta tcccatgggg
    37501 taaaacacgt ttgtataata tgatccgtga ccgcggcgat tgggtaattt cccgtcagcg
    37561 tgcatgggga gtaccacttc cgattttcta tgctgaaaat ggagaagcaa tcatcacacc
    37621 agaaacaatc gaacatgtgg caaaattatt tgctgaacat ggatcaatta tctggtttga
    37681 aaaagaagcg aaagaacttc ttcctgaagg atttacccac ccaggttctc ctaatggcga
    37741 atttacaaaa gaaaaagata tcatggatgt ctggtttgat tctggttctt ctcatgaagc
    37801 tgttttacgt cagcgaccag aattatcatt cccagcagat atgtatttag aaggatcaga
    37861 ccaatatcgt ggctggttca actcaagtat cacgactagt gtggctatca atggtgtagc
    37921 accatacaaa tcagttattt ctcaaggatt tgctttagat ggtgaaggcc gtaagatgag
    37981 taaatcttta ggaaacatca ttgcaccaga aaaagtgatc aaacaatttg gtgcagatat
    38041 ccttcgctta tgggtatcta gtgtagatta tgaagcagat gttcgtgttt ctatggatat
    38101 cttaagtcaa gtggcagaag tatatcgtaa aatcagaaat acgatgcgtt tcttattagc
    38161 gaatacagaa gatttcgatc ctaaaaaaga tactgtttcg tacaatgatt tacgttctgt
    38221 agacaaatat atgaccgttc gattgaacca agtaatcaaa gaaatccgag aagaagggta
    38281 cgacaaatat aacttcatgc atatttaccg tacagttatg aacttcttga cggttgatct
    38341 atcgtcattc tacctggatt ttgctaaaga tgtagtttat attgaagcag aagatgatta
    38401 tcaacgtcgt tgcatgcaaa ctgttttcta tcaaacagct gtagctttga caaaattatt
    38461 gactcctatc attccgcata cagcagaaga aatctggtca ttcctgaaag aagaagagga
    38521 gtacgttcag ttaagcgagt ttcctggtta tgaagagttt gcaaaccaag aagaattgat
    38581 ggatacctgg actgctttca tggacttccg tgataatgtc ttaaaagcat tggaagaagc
    38641 aagaaacagc aaattgattg gtaaatcttt ggaagcaaaa ctaactgttt atccaaaaca
    38701 acaagttcgt gaaatgttaa aagctttgga tgcagatatt gcacaattat tgatcgtttc
    38761 accagactac tttgaagtta tggcagcaga tgaacaagtg ccagataacg ctatggtatt
    38821 tgatgatgtt gctattacag tggaaaaagc agacggggaa acttgcgatc gctgtcgcca
    38881 agttcgtaaa gatgtaggag tagatgaaaa acttcctcat ctatgcggtc gttgtgcaaa
    38941 aatcgttgaa gacttctatc ctgaagctgt agctgaagga ttcgaagaaa agtaaaaggg
    39001 aaaagggggt gtgacatgtt ctttgtcaca cccccttttg ttatcaatcg tttaattttc
    39061 cacgttcacg ataccagtta tctggttgcc agatccattc gaatccgtct ttttcaagta
    39121 gttcgaaagc tgctttaggt cccattgatc cagctttgta gttgggaaaa tccggttgtg
    39181 tttggtccca agcttttcga atgacatcca cgatatgcca agattgagcg acttcttccc
    39241 aatgagagaa gttcgttcca tcaccattca acgcatctaa tagtaatttt tcgtaagctt
    39301 ctgggctatt ttcaaccatt tctgcactat ttctaaattc caatttgatt ggttccgttt
    39361 caaatccttg ccctacttct ttcccattca aggataaaga aaatccttca gttggctgaa
    39421 tatagatcgt tagaacatta ggtggaagtg tcgtatcgtc acatggttca tcgacagatg
    39481 ttttgaatac attaacagga acttgtttga atacgatgtt gatccgagtc cctttttcag
    39541 tcaaacgttt gcctgtacgt acatagaaag gaacacctga ccaacggaag ttatcgatag
    39601 tgaatttgcc tgcgacgaat gtttcagtct ctgattgagc atccactttg tcttcttcgc
    39661 gatagccctt gaattgtaca ccatccaatt caccttgagc atattgcccg cgaacgaagt
    39721 tttctaacac ttcttcttcg ttataaatgc ggatagcttt caatgctttg atcttttctg
    39781 tacggatttc tttttcggaa aatgctactg gcggttccat tgcaagtaat gctacaacct
    39841 gtaaaatatg attttgcacc atgtctttaa gtgcgccact atgatcgtag tagcccccgc
    39901 gatcttctac accgaggttt tcgccaaaag taatttggat attgtcgata tagcgattgt
    39961 tccattgagc ctcaaaaata ttattggcaa aacggatagc tgagatgttt tggatcattt
    40021 cttttcctaa ataatgatca atcctgaaaa tatcttgttc tgggaatact tctctgattt
    40081 gctggttcaa ttcaaaagca gactggtaat cagaaccgaa cggtttttct atgatcaaac
    40141 gattgaagcc attttttgtc ataatatttt gtgatttcaa atgagaaacg atcgttccaa
    40201 aaaattgagg tgccatagcc aaataataga cacggttacc gtttaattga tagtgtccat
    40261 ctaatcggtc agacaattct tttaatgtct gataatgcgt tgaatcattt acatcatggg
    40321 attgatagta aaaatggctt gcaaaggtag ttgcttcttc atctgaagga ttcaatgcac
    40381 ggattgtttc tttgacaact tcacggtagt gctcatcgct ccagggacgg cgagcggttc
    40441 cgattacagc aaaatgctcg cctaaatttc cttttctgta aaggcggaac aaagacgggt
    40501 acaatttacg ctgggctagg tcacctgtac cgccgaatat cgtaaacaat acattttttt
    40561 cattagacat gtggatacac tcctttatat tttatcgaca aaaatcgtgc tggccgcctt
    40621 aaagctgacg gggattttct ttctatcatt ttggatagtg atcggtcctt cataagctgc
    40681 gatgtcaacg atctcatatt cttcgttgat attcaggtca atagtgacta agtaatccaa
    40741 caattctttt tcatcaagca ctcgtgcgat acgcactttt gtaccgacag gataggactc
    40801 caatgtttgt cggcgttctt catggacaac ttcctctttt ttaggaatcg atcctccatg
    40861 aggacaatgt tcaggatgat tcaaatagtt ttctaaatga tctgcaagat gttccgaagt
    40921 cacatgttcc aatacttcag catcatcatg gacttcattc cagctatagt ctaaatgttc
    40981 aactaaaaaa acttcccata agcgatgttt gcgaatcaga ctgctagctt tttgcagtcc
    41041 gcttgcagtc aattgtacac cttgataagg ggaatgttct acaagctctt ctttcacaag
    41101 tttagaaatc atttcactga cagatgcagc tgagacatct agactagata cgatttgttt
    41161 attgttgact ttcgttgtat ctccacctag ttctaaaatg attttcaaat aatcttctcg
    41221 atttggtgtc atattcgatc atcctttcat ggtaaaaaac catcattagt aagtgtaaca
    41281 aaaaaaacgt taattgacta tttttctaaa taaaaaacaa aggattttta gacgttttta
    41341 caacaaaaaa cgaaagatga tagagaagac tcattctttc gtttataaag gatatatata
    41401 tacattggga gggatctatt tttcagataa caacttatat gaagtgtctg cagattcgtc
    41461 ataaagagtt tgttcgggat aagaaaagat aagataaaaa ccatactgat cacgtacgac
    41521 taaacgatat tcatctattt ctaccacaga tgtgccaaga gactgaccgt tgatcaaaaa
    41581 cgtgtaatca gatgtaatct gtaattgttg tttttgctgg ctatcactaa agaaccatgt
    41641 accttgatac tttgagagtc cttctgactt ttccgcttgt tttttctttt tcaagtaaat
    41701 aaaacttgag agaacggtaa tgagtgaacc aagaaaatag atcatttttt ttatcaccaa
    41761 atcactcctt tatgagaaaa taatcgcata gacatattct ttcttcttta tttcattgta
    41821 ccgaataaaa gctaaaattt ctctaagact ttcgactggt ttttttactg tttttctaca
    41881 aattatgata ggatacatag taggaagaaa ggtgacataa tttgaagtat cagcaagtgg
    41941 aaatagcttt ttttattgac cgcccgaatc ggtttatagc acattgtatg aaaaaagacg
    42001 gagaaataat tattgcgcat gtcaaaaata caggtagagg aaaagaggtc ctgataccag
    42061 gcgctgaagt ggctattgta tttgctcctg gacctaaacg gaaaaccgcc tatgatctga
    42121 ttgctgtaaa aaagaagggt gactggttta atatcgatag tcaattgcct aatcggctgg
    42181 caattgatgg tattttagac ggcacgatcc ttttaccagg attaactggt ccatttacgg
    42241 tatacaagcg tgaagtaact tatcttcact ccaaatttga tatttattta gagacggtgg
    42301 tcggacaaaa agcatttgtt gaagtcaaag gtatgacact tgaaaataaa gcgattggtg
    42361 cttttcctga tgcgcccaca ctcagaggat tgaaacatat aggagaatta acgtatgccg
    42421 cacaagatgg ttatgccgct tatgtcttgt ttattgctca gttcgaacat cttcatctgg
    42481 caacgatcca tgaagaaatg caaccagcac tagctgatat ggttcgacat gcgcagcaaa
    42541 gcggagttca aatcctcgcc tataattgtc aagtatcggc agatgaggta actgtcaagc
    42601 aggccattcc atttgatgta gatatccctt ttgaagatcc tatacaatag aaagaaggta
    42661 aaataatgat caacttaggt attatcggaa caaattggat tactcatcaa tttgtccaag
    42721 cagctttaag cacaaaaaaa tatgaattga cagcggtata ttctagacat ttggaaaaag
    42781 cacaggaatt tggttctcat tatgaagggg atatcgaatt cgcaacagat ttgaagactt
    42841 ttttcaatat tactcatatg gacaccattt atattgcttc tccgaatagt cttcacttta
    42901 gtcaagctaa gcaagcaatt ttggcaggaa aaaatgtgat agttgaaaag ccagcttttt
    42961 cgacgccaaa agaaatggat gaaatcatcc atttggcaaa tgaacacaat gtgtattttt
    43021 ttgaagcagc aagaaatatc catgaatcag gttttaaaaa gattgccgat tacttaccat
    43081 taaaagacga gataatcggt gcaaatttca gctatatgaa atattcttcg cgctatgacc
    43141 aagtattaga tggagaagaa ccgaatatct tctcgactca tttctcaggt ggtgcattga
    43201 tggatttagg tgtttacctt gtatatgcag ctgttgcttg gtttggaatg ccaaaagaag
    43261 tccattattt cccaagaaag atttctactg gtgtagatgg tcttggatgg gggatcttac
    43321 gttatgagaa ttttgatgta tcgatccagc caggtaaaat cggtgattct tatttaccgt
    43381 cagagattta ttttgactca ggaacgctag tgatgaatgg agtcaatgca attgaacaag
    43441 cacaatttca tgatcgcgcg cataaagaaa tcattgattt ggcggtaaca gctgcagaaa
    43501 atccaatggc agatgaagca gaggattttg ccaatgtcat ggctcatccg aaagatccag
    43561 cttggggatt gttgtatgaa gagtggatcg agctagctag aaatgtgaac caagtgattt
    43621 atgatttaag aaaaaatggc ggcatcaaat tcgatgcaga taatgaggaa gatttttaat
    43681 gaacgaattt gtacaagtat ttcccgaaaa ttggcagaaa aaatatgagg ataaagggtt
    43741 tatctcacct tctcttattc aacgagcagt ttttcagcca ctaagtaata aacaaagtat
    43801 agtagcggtt tctcctacag gttcaggtaa aacactggct tatctttggc ctctactact
    43861 aaacgtggaa ccaggtgaag caagtgcgtt ggtcattttt gcttcttctc aagaactagc
    43921 tattcaagta gcagatgttg cacgtgaatg gggaaaagac aaagagttga aagtccaatc
    43981 tttggtgggc ggagccaacg tcaaacgtca gattgagggt ttgaaggaaa aaccagaaat
    44041 cttgattggt acaccaggaa gaatattgga attgatgaaa gcaaaaaaaa taaaagcaca
    44101 tcaagtgaaa acaatggtat ttgatgaagc agaccagcta tttgacgctg ggaacagcca
    44161 aatcattgat caaattcttc atcaagctcc aacagagtat cagctagctt ttttttcagc
    44221 gacagcagat cgttcccttg aaagcattga aaaaatcact gggaaaacgt ttgagacaat
    44281 tgacgtgaca gcagaggacg attctcgaaa aggactgaga cattactttt tacgtgtacc
    44341 gatacgaaag aaagatgact acttgagacg tctcacgcat atagaagatt ttcatggtct
    44401 gattttcttt aatcaactga ctgaattagg cgtaatggaa gaaaaattgc tgtatagagg
    44461 tgtaccggta ggaagtctgg cctctgatca gaacaagcta ctccgaaaag cagcgctaga
    44521 acaattcaaa aagaaaatgg tcagagcact tttgaccaca gatgtggcgg ctagaggttt
    44581 agatattaca ggtcttcctt atgtagtgaa tgcagaagtt ccgttaagtg aagaagctta
    44641 tttgcatcgt tcgggaagaa ctggtcgaat gggtaatgaa ggaatagtaa ttacactcgt
    44701 acaagataat aatctaagag atttaaaacg gcttttacgt ccatcaaata taacacttga
    44761 agagattttt ttatacggag gtaagctgca aactgagcag ccaaccaaag aagatgcagc
    44821 tcctgcagta aataaaaagt atgcgaaaac tttttctgga aaggagcaga acccagaata
    44881 taacaaagaa aaaataagaa aaaaagttaa aaataaatca aaaaaagaga gaaataaagg
    44941 agcaaggcgc aaataagcgc ctttttatgc tatgattatg aaaacgggaa caaaattggt
    45001 tgccagagga ggggttatgt gaaagaacgt caagaagaga tcttgcagct attgacagaa
    45061 aaccaacaag gattgacagc aagtgatgta gctgagcgct taactatcga tcgaagcaat
    45121 gcaagccgat atttaagtga actatacaag gcccatcata ttgtaaaaac cgctggccga
    45181 ccagtcgttt actctttgcc aacggaaaag tcaaaatctg atgaagtgca tgtcgactct
    45241 tctacgcaag tgacttttga aacactcgtt ggagaaaatg attctttaaa agtcagcata
    45301 cagcaggcaa aagctgcgat tctttatcca ccgcgggggt tgcatacgat tatttttgga
    45361 gaaactggta cagggaagtc gatgtttgct gaatgtatgt atcattttgc aatcgattca
    45421 gagatgcttt ctgcagatgc gccatttgtt tctttcaatt gtgcggatta tgctcaaaat
    45481 ccgcaactgc tctttggaca catctttggg atcaaaaaag gggcttacac aggagcagct
    45541 caagatagtc ccggtctaat agccaaagca gacggcggta tattgttttt agatgagatc
    45601 caccgtttac cgccggaagg gcaggaaatg ctgtttactt tcattgataa aggtatttac
    45661 cgtccgcttg gagaaagtgc acaagtacat gaagcatctg ttcagatcat tggggcgacg
    45721 actgaatctt cagaaagttt cttatctacg ttcaatcgac ggattcctat ggcaatcacc
    45781 ttgccgaatc ttgcttcacg ttcactggat gagcgttatg aaatcatctc tctttttatc
    45841 aaacaagaag cgaatcgttt gaatcaacgt atcgatatcg aaaaagaagc gattcttgcc
    45901 ttcatgcttt atgatgcgga aggaaatatc ggtcaagtga aacgagactt gaaactagtc
    45961 tgtgcaaaat cttttttaca ttaccgtact catcatgaga aaaagctaat cattcgaaaa
    46021 gaagaacttt cccttcaagt acaaaaagga ttactgaaaa tcaaggaaat accggaacgt
    46081 ctcgaccgat tcatggacag caaaagtcag tatttgacat ttgaaccagg atttgctgat
    46141 gttgtctggt cacaagatcc agaacgaaac atgcaggtct ataacgatat tgaggaaaaa
    46201 gtcacttctc tttccgaaac cggtgtggag aacattgatc tagagaccct catttcaaaa
    46261 gatgtggatg cttatttcca gacatatgtc aaagaattga ctaaaagttc gattcccaaa
    46321 gatcttttgc cagatgaaat ctggcggtta accaatcaat tgtatgatgt ggctgaaaaa
    46381 cggttagacc gtatttataa tgaaaaagct cgttttgctt ttgctttgca tctgcaaagt
    46441 acattggatc gtgtgagaga aggtcatatg atcgtccacc cagatttgaa taacgtacgg
    46501 aaaaatttta agagggaatt ccaggtagca atcgatcttt caacgatcat tgaagaagaa
    46561 gaacaagttg agattccttt tgatgaaatc ggctttatta gtatgtttct ttctatcaat
    46621 ctaggagaaa gtgagcctgt taaagaaaac aaagtagatg ttctcatatt gatgcacggc
    46681 agacagactg cttcaagcat gttggagatg gcacaagaac tattaggaac aaaagtaggg
    46741 aaagctttta atatgccact ggttatggaa gttcaaacaa tgtatgaaga agtgttacaa
    46801 tatgtcaaaa actatcaaga gcatttgacg aatggcctac tcttgttaac agatatggga
    46861 tcattaaaca ccttcagtga tttgatttat gaagagactg gcattcggac gaaagctatc
    46921 acgatgacaa gtaccatgat tgtgatagaa gcgatacgga tggcaagtgt tggaagaagc
    46981 ttagaagata tttatcaaaa tatccagctt tcctttgaat cgatcgtacg tgatcagttc
    47041 aaagcggaaa agtctgaaac tttacagcgt aaaaaagccg taatcgtgac atgctttact
    47101 ggtgaaggag tagctgccaa attatatcag cgtattatgc cagtgattaa tcaaaataag
    47161 gtagaactga tccagatgca gtttttagaa cgtgaatcat tcaaaaaaca catcgatgcc
    47221 ttattggaag aatatgagat caaagcaatt gcgggaacag ttgatgttga ttatcaaaat
    47281 atcccatttt tttcagccta tgacgttttt gatgatgaaa aactaaatat tttaaagcgt
    47341 atagctgatg atgagatccc gacagctaaa attgtcgatt cacttgctgg atcattaagc
    47401 gatgtctttt ctattgatga actgatccgt tcattgcaaa agacagttca gcagatccag
    47461 accgatttgt cgattatcgt ggaacctgga gtagatactg gaatcgttat ccatttagcc
    47521 ttcatgttag acggattgat caaacaagaa agccatcggc aatttgagaa actgtctatt
    47581 ttctcaaaaa tttatcgttt ggaaatggat atcgttcggg caaacttgat ggcactagaa
    47641 cgaaaatatc aggtaaaaat tccagaagat gatatcgcat ttcttacaca aatgtttctt
    47701 gaaaataaaa taaaaaaatg aacacactaa tcatactgtt tacacactaa acatctgtgt
    47761 gcgcactata aaaaagagct aaaaagcatg aaaacgctat ttcagctctt ttttttattg
    47821 gcatgcttct tgcgttatat aaaagtgtaa aaaaataaaa ggagtgttaa aaatggcaaa
    47881 aaaaacaatc atgttagttt gttcagcagg gatgagtacg agtttattgg tcacaaaaat
    47941 gcaaaaagca gcagaagaaa aagggatgga agccgatatt tttgcggtct ctgcctcaga
    48001 tgcagataat aatttagaat ctaagaatgt cgatgtatta ttattaggtc ctcaagtacg
    48061 tttcatgaaa gctcaatttg aacaaaaact tgcgcctaaa ggtattccat tagatgtcat
    48121 caatatgcaa gactatggca tgatgaacgg agaaaaagtt cttgcacaag cagaaaatct
    48181 aatgaaataa atcaagtatg cagttggggg tataacaacg tggaagatca acaaaatcta
    48241 gaagccatca tggggttaat catgtttggc ggtaatgcaa agagcgatgc aatggaagcg
    48301 attgcggctg ctaaaatcgg tgattttgag ttagcggata ggaaaatcgc tgatgcagaa
    48361 gaatcactta tacaagcgca tcatgcacaa acagacatgt tgacacaaga agcacaagga
    48421 aatcatatgc aagtgacatt attgactgtt cacagtcaag atcacttaat gacatctatt
    48481 gcgtttactg atttagcaaa agaaatcatc gaactttatc gtcgcgtata cagcgaataa
    48541 atacaacttc ttttttgaac ggaagaagaa aaatttttga acggattttt tattttgtta
    48601 taaggagaac tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat
    48661 caagttggat ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt
    48721 tagttgcttt gcgtgatgct tttatcggca cactgcccgc aacaatggcg ggatcagtcg
    48781 ctgtaatgat caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg
    48841 acagtttagc agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg
    48901 gttttgtctg gaacggtaca ctagcaatcg ccggattgat ctttgccttt tcttgggggt
    48961 ataatcttgc ccgagcttat ggagtaaatg atctagctgg ggggatcgtt ggtttagcta
    49021 ccttgattca aggtatttcc tttggtccag cattatcagc agcattagat gtgaatgttc
    49081 cttcaaatgt cgcagatgca atcaatagtt ctgagattgg tgcaacgatt gcagatggca
    49141 cactgtcagt tggattatgg ggttggctaa aactagatca tttgaatgga aacgcttatt
    49201 ttacagttat gatcatggga gctatttcag tcattatttt ttgtaaattg atgcttgcca
    49261 acattacgat caaaatgcct gattcagtac caccagcagt atctaaagca tttgcagcca
    49321 ttttaccagc gacgatcgct ttatacgtca ttgctattat caattatatc gtaggtcgtg
    49381 tagctgatgg gcaactgatc attgatttgg tgcaaaaata tattgcagaa ccattcctag
    49441 gattatctca agggttggga gcagtattga tcgtcacagt atttgttcaa gtattctggt
    49501 tctttggtat tcatggacca aatgtattgg caccagtgct agagggaatc tggggacaag
    49561 cacaactaat caatattgat attttccaaa aaggttataa cggaaaaact ggtacaccag
    49621 ccgtgttaga tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg
    49681 cttttgcatg gtttggcgga tcaggcggaa cgatcgtctt actgatagct atcttgattt
    49741 tctctaaacg tgcagattat ctaacagtag ctaaattatc cattgggcct ggtatcttca
    49801 atatcaacga accagtgatg tttggtctgc caatcgtatt gaatgcaatc atgtttatcc
    49861 cattcttgat tgctccagta gtagcaacag caattggatg gacagctact tatcttggcc
    49921 tagtcgctcc ggtatcgcag caagtcacat gggtagtacc gccgttctta ttgtcattct
    49981 tagcaacagg agcggattgg cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt
    50041 tcttgatttg ggcaccattt gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac
    50101 aacaataaga tgaacgtgag gagcgggacg ctttaagttt cgttcctcat aatgaatttt
    50161 ctaggaggaa ctgaaatgat tacaattgcc agtgcaatgc aacaagatga aatcaaagaa
    50221 ttgttagaag gatacaatga agaaggaatg acttttacat ttaaagaaaa acaaggaatc
    50281 aagctgttct ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa
    50341 ctaatcaaag cacagccttg gggaacagtc ctttattttc aatcaacagc tgtttagaaa
    50401 atggtttaaa aagataggat ggttgagatg ataaaaaacg gtttattttt atgtagtatt
    50461 ggttctttga tcgtgttgta tagtttgaat ccacattcgc aaaggtatga tttgtatagt
    50521 atggtcacag gtctttttct aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa
    50581 agagaagaag caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag
    50641 catcgttttt ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg
    50701 gcaaatcagt aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa
    50761 ataaccgggc tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta
    50821 catcgacaac gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga
    50881 cctgtgaggt tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg
    50941 atatgctttt aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag
    51001 atatgatcga agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat
    51061 gaagtaagct agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag
    51121 cttttttgta ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc
    51181 gattgaggaa atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag
    51241 gcgagaacgt tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga
    51301 ggatcggcta acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga
    51361 gtacctaatg gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg
    51421 gatcaatcag atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc
    51481 ttatctggcg ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga
    51541 taaaatcagt tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa
    51601 aataaagcgt atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat
    51661 gccggaaaac caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa
    51721 gcaaaaaggc aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata
    51781 tatcgtttat cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca
    51841 tgatgctttt tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt
    51901 tgaatattca tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc
    51961 ttctgtaatg atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga
    52021 aaattatcca tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta
    52081 tgcgaaatgg aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc
    52141 aaaagtagaa caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct
    52201 attattaggg tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa
    52261 agaatatgaa gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta
    52321 tttttgataa aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg
    52381 gcatttgaag gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt
    52441 tcttttgttt tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc
    52501 cgattttttt tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc
    52561 attggaaagg acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac
    52621 tagtggaaac gttttgccta acttataagc tgattttttt agcagataag cttgcaagta
    52681 aaaaacaaac aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag
    52741 caatcgcctc ctaagcgata ggtcgctggt tcaattccag tcaggaacgt ataaataacc
    52801 cctgtatcct ttgcggtaca ggggtttgtt ttgtatttgt gccaaaaatg tgccaaacta
    52861 gttatcattt ttatataaac tatcgaacac agtattgatt ttttctttat cctgatcttc
    52921 aagctctcga acaatatgaa gataagtaga cattgtagtt tctaaactgc tatgtcctaa
    52981 acgtttcgat acgctaagta tatttactcc ttgataaagt aaaatagatg catgtgtatg
    53041 ccttagtcca tgcaaagtaa attgcttttc aatccctaat tcttttaatt ttttgcgcaa
    53101 atatttgctg acggcgtttg atgaaactaa tccatttttt aagttgaaaa aaacaaaatt
    53161 atgagggttt ttgatttcaa aattctcgaa taattctttt tgattgattt taaacttttt
    53221 taagagatca atcgtatgtt tatcaatgga aattttccta tttgaagttt cgttttttgt
    53281 ttttccccat tcatttaatt tataattcca tgttttatta atagtgatga tttgatcttc
    53341 gaaatcgata tcttcccatg ttaatcctag aagttcagca aatcgcattc cagtggcacc
    53401 agcaactaac acgagcatag gagaagagta ctgcgcagaa aggttttctt ctacgagttt
    53461 cattaaagat ttaaattgat cataatctaa atatttatct tcttttggct tcaatgaatc
    53521 ttttcctttg attactgctt ttctagttgg atcaaacgga attaatcctt cttctacggc
    53581 atctttcaac gacgctctaa tatggttatt aaacttaata acggtagatt ttacatggtc
    53641 tttagcatac ttatttaaaa agcgttgata tcccactcta tctaaatcag aaatcaatac
    53701 cgctggcata tatttcttta tgttcattaa cgtatcttcg tattttctat aagtgatagg
    53761 agatactgtt ccttctttat aaagttgcat ccaatcctca aagtaatctg ataataatag
    53821 atttttgcga tcccctttaa ggcctttgtt caagttatat tctaattcgt tggcggcatc
    53881 ttttgcttct ccttttgtct taaatcctga ttttcttatc ttcgtgtatt ttccgtcatc
    53941 ttttttgtat gaaatttcgt attgccaact atttccacgt ttgactaatc ttgccataat
    54001 tgaatcagct ctctttctct gatacaatag gaactataaa gaagcccatt gtttgggttt
    54061 tattattttt tgcacatcct ttttgcttgc cggcgcaagg atgtgctatt tgtcgtttta
    54121 atggtataat tggatgtgta ttagatcatc caatcagagg tgtttattat gattaaagtg
    54181 atttttaaaa gaataactga acttccagag tttagattat ttgttgatta ttggtatgtc
    54241 tggtttttta ttgttgcatt gctgataggt ctagatgtca tattagaaag aaaacttaag
    54301 taagagttga gttaataaat tgaattccta aatgagatat tttgtagccc atattttgtt
    54361 tgccgacatc acctaatact atggagctaa tcgttttttc taacagacca aaatcagata
    54421 atctatttaa gctaccatct attagctctt gtgaatagcc agacatgctg atatttccag
    54481 tgaaattttc gtttccacga ctcattaagc atgctaacaa ttgaatctca atcgatgtta
    54541 attgattcag tacaagaaga taatactcct cgttatccca tgaagcgctg ttaattcgaa
    54601 gtaaacaatt cttataaaga tttttataca tatctatttt attttgtgcc tttgattttt
    54661 ctacctcgtc atttatccct tcgaatattc caatgagttg gtccttgttc tctaagttga
    54721 aaacatcttc tggaatttga tcgcgaatgt tttctaatga ttcgtttaaa tttttgtaga
    54781 atttctcgat gcgtttgaaa cgcttctcgt ttttagaccc aaaataaagt gactgcacaa
    54841 cgccaccaat ataaggaatt gctccaatac cgccttcaac tattaattca agatattctt
    54901 ttttggttag tttttcattg ctattatcca tagttacctc ctattttgtc atttccttta
    54961 caagcattgt gactcctgat ggcactcttt tgtataggtt ttattattgt gcacaatcta
    55021 actcttagtg gggcgtggtt catttatttt ttacttactt tactattaat catatcccat
    55081 tgagcatagg atttttctat gttcgatcct cttgaactaa tttctcctgt aaaagttact
    55141 ttatcgccct ttgtaaaggt atcagcattg tttaagtcct tcgcaaaaat tacatatgaa
    55201 actttaggtg ttgcagaaat atcattccaa cttttatcat cgtactcttt gtctgctata
    55261 atggctattc tagtagtcat tgattcgata acagttcctt caaaagtata tgttttacca
    55321 ctaatatatt tagtaaaagc tgtattttgc tcagtagctg gagtaataga ataataacta
    55381 tctaagaaag caccccagtc atgattattt gtctctaaaa attcttcaaa ttgagttagg
    55441 cttttattgc tgctgttaga tgtagtagtt tgagtatcag atgatgagct ttcctgtttg
    55501 ttattgtcat ttgcctcgtt tgatgaggaa agcgagaaac ctacggtagt aaaaataatc
    55561 ccgataataa ggagaatcat aggtgttttc ttatttgttt ttttgataaa tcttacaatt
    55621 aacatgatta aacctataat aaataaaaca ataccagtaa aaccaaatat accgtccatt
    55681 aatagatcct ctttctcttt tgatattgaa tattataaac caagcaattc ttttttctta
    55741 aaatcgaatt cttcttgatt aataatccct tcatctaaca actctttata ctttttaatt
    55801 tcatctgcac ttgaaatttg tgtttctttg gaattattat ctaatataga ttgttgcttc
    55861 tctatatatt tttttaaatt ttccatttca ttccaatatt tttttgtaaa cataacggtg
    55921 ttttcatctt tcgtggccgc taatatacct ccgcgacttt cgtttccgcc tagcaaggta
    55981 aattgtatat aaccattact catacctggt ttttttaatt gtacggctga tatattttta
    56041 aaggggatag ttttttctcc tttaatacct tgattaacta aatttataaa tccttttcta
    56101 ataattgaaa tagaatcatc agttacttta atcaaggttt ttcctggaga ttttattaaa
    56161 atctcttttt ccattttttc ttcctcattt ctgtgatatg attttttttg tagaagctca
    56221 tatatgagga aagagcccgt gttgcagcac gggctttttt aatgttttgc tgaaatcggt
    56281 ttttttaata attcttgatg tattcataca tattgccttg cgtaagaata ttttttctta
    56341 aaatagcatt ggcagacagc ataacaagtg tatctgagct tattctgaat agaataatac
    56401 tccataaatt tttcgagatt aaactgagat tcatcagtca gttcgttctc aatatagata
    56461 tttaataaaa ttagaatagc tattttatct gcttctgttt caaattttga gtgaaaagtt
    56521 gtagaagtat cgtacaaaac tgaaaattca aaatgagagg cgctgaaatg tgcaagttca
    56581 tgagataaat gaaaggcttc tgcagtttca ccgtatagat tttcattcaa aaaaatgatt
    56641 ctgggttttg gatagtagaa accaggctct ttcatctcca tataaactac tttcaaattg
    56701 tattcgctca acatttcttt caatttcaaa tacatacaaa ccatcactcc aactattcat
    56761 tttcctctaa agctttagca attgcaatcg ctttacgcat tgtctcctta gatatttctt
    56821 ttccatcaaa agaaaaaaca gtatcgtctt ctgataaatc cacatgttta ggggtttctc
    56881 tttcttctct acctagaagg tagtctacag agacatcgaa atagtcagca atccgactta
    56941 attcatctga attaggagta ttatttttcc actttgctag ataaccgtta gaatatccaa
    57001 aattaatttc taactgccgt atagatatct ttttcctctt cgccaagtct tttattattt
    57061 cataggtatt catcgataaa acaacctttc taaatgttta caaaaaaagt ttagaaaaat
    57121 acgcagaaat aatttgacta attctgagta atgagctata ctatatcttg taaacagatt
    57181 taacaactaa aaagacaaca aaaaacacta ttgatatgta aatgcagacc gccaagaaag
    57241 ctttaaaatc aatgttttta tgtcttattt aattatgctc ttagtataga gtattactca
    57301 gatactgtca attgaattta gaaaaaagtt gttaaatttg tttacgaata taaaagaaag
    57361 gaatgagaaa tatgaacaca ccgcaaattt ttaatttcga acaaaacgaa gttcggacaa
    57421 ttttagtaaa tgatgaacca tattttgtag gcaaagacgt tgcgagtgtt ttgggttatt
    57481 caaacactaa agatgctttg tcgcggcatg tagatttgga agataagatg gggtcgcgaa
    57541 tcacgacctc aggtcaatca agagagatga caatcatcaa cgaatctggt ttgtacagtt
    57601 taatcttaaa atcaaaactt ccctctgcca aaaaatttaa acgttgggta acaagtgaag
    57661 tgttgccagc aattagaaaa catggaggtt atctaactcc agaaaaagta gaagaagctt
    57721 tgcttaatcc agatacaatc attcaattag caactcaact aaaagaagaa agaactggaa
    57781 gattaatcgc agaacaaaag attgcagagt acgaacctaa aatctcctat ttagatagca
    57841 tattatcttc tacagattca gtaacaatta gtcagattgc agcagattat gggatgtctc
    57901 cacaacagat gaataaatta cttcataaac taggtattca gaaaaaagtc ggtaaccaat
    57961 ggttattgtg caaaaaacac atgaaccaag gatacacaaa atctcataca actgagatcc
    58021 cgaaagccga tggtggcact aaaattgtaa tgaataccaa atggacacag aaagggcgtc
    58081 tatttatcta cgaattacta aaaaaagaag gatattaccc tcaaatggat ttagaggaaa
    58141 ttggttagaa aggagtttta gtatgactga cattgcagaa atcactcaac gagatagaga
    58201 aaaaatcaaa gaatatgtcg aaagttcaaa gttcttaact tacaccatgc ttgctgaaag
    58261 atttggaatt agcaaaagct acttatcttt aattttaaac ggtaaaaaga cttctgcaga
    58321 agcaaacaga attatagatt cgattatcac tatgtacgaa ttgtaaaggg aggaacagct
    58381 aatgcaatat ctagaagcaa aaatcccaat tccagaaggc tatgtaatta tctcccaagt
    58441 ggattatgag gagttaaaaa aagctgatga tactggtaga tggatgacgt tgccagaagt
    58501 gctagaacgg attaacagaa aatatgattg gtttacttct agagttttaa agaacccaag
    58561 atatagaaat attatcgata tcgagaaaaa caaaaatgga tttgtctatt atccagttga
    58621 aggaagagac acatatctat ttttaagaag taaaacactt gaatttttag aaacaaattt
    58681 ttcggaaatc ttaaggaggc aagcggatgg gaaaatttaa tagagcgcta gtgttcagcg
    58741 caccgctaat catctacgct ttaggacttt ggggaagcag gcaagcgttg ataggaacga
    58801 ttgtttacat ggtttggatt tttatggggc ttgatgaagc tgaagctgag tacagagcga
    58861 aaaagccaac cgagggggct gactaaaaat gaatagaaaa gagaaactag aatgtatatt
    58921 actattactc agtttaattc tttcactaat ttctctattg ggaagtttgt atttttgata
    58981 tcaacaattt tgtggatttg ggttctattt agagtaaata tcatttttgt ttggtgatta
    59041 aggagtttat taaaattaat tttccctact tcaaatgcaa ggtaacaaga tatagcactt
    59101 ttaggaggaa tatttagcgg tacagcttca gagtatattc tattatcatc gactgatcct
    59161 cctttagcaa ttaatttctt aaatggagta gctgaccatc tgcggtttaa gttagcggat
    59221 gaaagttcta aattaactaa catagctggt tctgttgaat agttagaaat gattactttt
    59281 gtataaatca tatctgcagc gaagtaagaa gcattaagtt caacttctag ttgagggcgc
    59341 attcctttta tttttatccc tgttaaaaac gtactcaata taaaacttat tattgacatc
    59401 cattgaaaaa ttgttaagtg aaaaaaattc aaaataaaca ccaccagttt ttaactaaat
    59461 tataccaaaa aggagagaag aaataatgca agaattagta attttgaaaa ataaagaagc
    59521 tgtgactacg agcttacaag tggcagaaag cttcgagaaa aaacatcaac atgttttaag
    59581 agatattgat gcactaaaaa aagatgtgtc caattttgga cagatgtttg tagaaggtaa
    59641 tgaaccagat tcatatggca gaaatcgacg agttttcttc attagtagag atggattttt
    59701 cttgctggct atggggttta caggaaagaa agctatctac ttcaaacaaa aatacattga
    59761 agcattcaac gaaatggaag atgttattcg caagaatact gttcctcaaa caattgaaga
    59821 tatgatgatc tatcaattag aagaaatgaa agatgttaaa aaagatgttt ccatgcttaa
    59881 agatactatg cgaattagcg gacaacaaga gtttgaaatt aagcaaaaag gaaatatgaa
    59941 agttatggga gttctagggg gaaaagaaag ccgagcttat gaagaaatca gcaaaaaagt
    60001 attctcaaaa ttttggtctg aatttaaacg taccttttca atcccaagat atggcgagtt
    60061 acctcgtaag agattcgatg atgctgtttc atttattgaa atgtggttgc cagaaactgc
    60121 gatccgtatg gaaattgatc aactgaacag acaacagaga ctttttggtg atgacaatga
    60181 atagagctga agcgctaaga atagggacgg caattgctaa ttgctggtgg aaatactata
    60241 aaccaatcat cctaagccaa caacatattg acaagcaaaa agcatggcaa caaataaaaa
    60301 agtgactccg ccggcaagca aagagtcaca aagaaaacac atcataagga gattttagca
    60361 tatggaaaaa gaactttcca ctctagatca atatttgatt gatcctgatt ggggcaagcc
    60421 gaaaattgag gaaacaagtg gtcgaaaaat cagacgaaat cttttgacga atgaagaact
    60481 agcttgggat caagatgatt taggcaacca tgtaactatt tgggatcatg tttatcttat
    60541 ccatctatcg aagcattcga ataaacctga atatatttac gtcatcgaag atggcttgat
    60601 tgatgcgcta gaagagtacg acagagataa cttgattgat atctcttatt acggaccagg
    60661 taagaaatac attgctgaaa tggaggcaga atttgatgag tgaaatcaaa gggacaacga
    60721 actttgaaaa actttttagt cgtaagttaa ataaaattct caagaaaaaa ggaaattttg
    60781 attatttatc ttgggctcac gcgtgggaga ttatgaaaaa gaatgatcca caggcaacgg
    60841 taactattaa tgagtacaaa cactacaggg ttgtttctgg aactcatcaa gactttcttg
    60901 ttgaggaata taaacctttt cttatggatg aaactgggac ttatgtatct gtctcagtaa
    60961 cggttaaagg acacacggaa accgaattat ttcctgtttt agattatcga aaccaaccag
    61021 ttgttaaacc aaatgcaatg caaatcaata actcattgaa gcgatgcttt gtgaaagcat
    61081 tggctctaca cggactggga ttatatgtat ttcaagggga agatattcca acaccaccta
    61141 gaatcgatac aaagaaatta aacatgctag agacgattct agaagctttc aatgagcaga
    61201 tgggtaaaga tatgaccaaa accttaatcg aatatgttaa tgagcagaca gataaattag
    61261 ggctcttagc tgataacgtt gaaactattg aacagttaag ctatgagcaa tgtgccttga
    61321 tggagcgagc aatagcagct aagagaaaag aattagataa gaagtgatat gagtgtttaa
    61381 accattaatc gattcatatt cagcggttct gaaaaagttc aaaggaaaag acataagcgc
    61441 aaccatcaat gaggaagtga acattgatcg actaaagacg atgtatgacg gctacgatgg
    61501 tgatcgagtc attgaaattc gttttattga tcctagacgt ttcaccgtac agcaacgaaa
    61561 cttcatctat gcgctgatag gcgatatttt tatcgataca ggcatgccaa cggacttctg
    61621 gaaggaattc ttctactttc gttttgaagg tgtcacaggg cgcaaaataa gcctgaaaga
    61681 cgaatcgaat acaactgtga gtgatgccaa tgtcttagca aatatcatct tagatttcat
    61741 ctttgaacat catattcctt tcaaagaagg ctatgagatt ttacctgcga atcaagaata
    61801 ttacttctac aaatgcatca caaaaagagt ctgctgcatc tgtggcaaaa caggagctga
    61861 catcgatcac tttgacaaag cgctaggaag acgaaagcgc aaagaagttg atcatgcaga
    61921 gtacacattt gcagcactct gcagaatcca tcacacagag aagcacaaaa taggtgtgat
    61981 taatttcaaa aataaatatc aaatcaaagg gatcaaatta aaccagaaaa caatcaaaaa
    62041 gttaaatatt ggagggtaaa aatgacagaa catcgaagtt attatgcgat tataccagcc
    62101 aacgtaaggt acgacaaaag acttaaacca aatactaagt tgttatacgg agagataacg
    62161 gccttgtgta atgaaagagg cttttgttgg gcaggcaatg agtactttgc agatttatat
    62221 ggtgtgaata aagagaccat atcgcgatgg gtaagtgatt tgattaagtt tggatacttg
    62281 aatcgggaaa tcatttacaa agagggtacc aatcaaataa tcaataggta cctacgaatt
    62341 aatcaatacc ctattgacga aaaacgcaat acccctattg ac
//
LOCUS       DASHRQ010000010        60533 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.010, whole genome shotgun sequence.
ACCESSION   DASHRQ010000010 DASHRQ010000000
VERSION     DASHRQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.010"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            232..663
                     /locus_tag="U2M88_000671"
     CDS             232..663
                     /locus_tag="U2M88_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEM8373075.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
     gene            complement(895..1152)
                     /locus_tag="U2M88_000672"
     CDS             complement(895..1152)
                     /locus_tag="U2M88_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373076.1"
                     /translation="MSKLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            1315..2289
                     /gene="bsh"
                     /locus_tag="U2M88_000673"
     CDS             1315..2289
                     /gene="bsh"
                     /locus_tag="U2M88_000673"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEM8373077.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            3019..3336
                     /locus_tag="U2M88_000674"
     CDS             3019..3336
                     /locus_tag="U2M88_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEM8373078.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(3540..3905)
                     /locus_tag="U2M88_000675"
     CDS             complement(3540..3905)
                     /locus_tag="U2M88_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373079.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            4113..4595
                     /locus_tag="U2M88_000676"
     CDS             4113..4595
                     /locus_tag="U2M88_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373080.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            4792..5682
                     /locus_tag="U2M88_000677"
     CDS             4792..5682
                     /locus_tag="U2M88_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEM8373081.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            6007..8478
                     /locus_tag="U2M88_000678"
     CDS             6007..8478
                     /locus_tag="U2M88_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEM8373082.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(8581..10317)
                     /locus_tag="U2M88_000679"
     CDS             complement(8581..10317)
                     /locus_tag="U2M88_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEM8373083.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(10314..11018)
                     /locus_tag="U2M88_000680"
     CDS             complement(10314..11018)
                     /locus_tag="U2M88_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8373084.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            11149..11652
                     /locus_tag="U2M88_000681"
     CDS             11149..11652
                     /locus_tag="U2M88_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEM8373085.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            11612..11950
                     /locus_tag="U2M88_000682"
     CDS             11612..11950
                     /locus_tag="U2M88_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEM8373086.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            11971..13389
                     /gene="ffh"
                     /locus_tag="U2M88_000683"
     CDS             11971..13389
                     /gene="ffh"
                     /locus_tag="U2M88_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEM8373087.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            13501..14079
                     /locus_tag="U2M88_000684"
     CDS             13501..14079
                     /locus_tag="U2M88_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEM8373088.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            14083..14604
                     /locus_tag="U2M88_000685"
     CDS             14083..14604
                     /locus_tag="U2M88_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEM8373089.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(14683..15237)
                     /locus_tag="U2M88_000686"
     CDS             complement(14683..15237)
                     /locus_tag="U2M88_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEM8373090.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            15490..15765
                     /gene="rpsP"
                     /locus_tag="U2M88_000687"
     CDS             15490..15765
                     /gene="rpsP"
                     /locus_tag="U2M88_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEM8373091.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            15777..16028
                     /locus_tag="U2M88_000688"
     CDS             15777..16028
                     /locus_tag="U2M88_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEM8373092.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            16153..16944
                     /locus_tag="U2M88_000689"
     CDS             16153..16944
                     /locus_tag="U2M88_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373093.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            17114..17635
                     /gene="rimM"
                     /locus_tag="U2M88_000690"
     CDS             17114..17635
                     /gene="rimM"
                     /locus_tag="U2M88_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEM8373094.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            17625..18386
                     /gene="trmD"
                     /locus_tag="U2M88_000691"
     CDS             17625..18386
                     /gene="trmD"
                     /locus_tag="U2M88_000691"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEM8373095.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            18522..18869
                     /gene="rplS"
                     /locus_tag="U2M88_000692"
     CDS             18522..18869
                     /gene="rplS"
                     /locus_tag="U2M88_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEM8373096.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(19001..20137)
                     /locus_tag="U2M88_000693"
     CDS             complement(19001..20137)
                     /locus_tag="U2M88_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM8373097.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(20253..20819)
                     /locus_tag="U2M88_000694"
     CDS             complement(20253..20819)
                     /locus_tag="U2M88_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HEM8373098.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(20955..21608)
                     /locus_tag="U2M88_000695"
     CDS             complement(20955..21608)
                     /locus_tag="U2M88_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373099.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(21658..22086)
                     /locus_tag="U2M88_000696"
     CDS             complement(21658..22086)
                     /locus_tag="U2M88_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEM8373100.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(22091..22465)
                     /locus_tag="U2M88_000697"
     CDS             complement(22091..22465)
                     /locus_tag="U2M88_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8373101.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            22769..22954
                     /locus_tag="U2M88_000698"
     CDS             22769..22954
                     /locus_tag="U2M88_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8373102.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            22967..23215
                     /locus_tag="U2M88_000699"
     CDS             22967..23215
                     /locus_tag="U2M88_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373103.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            23246..23416
                     /locus_tag="U2M88_000700"
     CDS             23246..23416
                     /locus_tag="U2M88_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373104.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(23518..23976)
                     /locus_tag="U2M88_000701"
     CDS             complement(23518..23976)
                     /locus_tag="U2M88_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373105.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            24144..24461
                     /locus_tag="U2M88_000702"
     CDS             24144..24461
                     /locus_tag="U2M88_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373106.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            24394..24873
                     /locus_tag="U2M88_000703"
     CDS             24394..24873
                     /locus_tag="U2M88_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HEM8373107.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            24890..25066
                     /locus_tag="U2M88_000704"
     CDS             24890..25066
                     /locus_tag="U2M88_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373108.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            25323..25754
                     /locus_tag="U2M88_000705"
                     /pseudo
     CDS             25323..25754
                     /locus_tag="U2M88_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25760..26077
                     /locus_tag="U2M88_000706"
     CDS             25760..26077
                     /locus_tag="U2M88_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HEM8373109.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            26196..26882
                     /locus_tag="U2M88_000707"
     CDS             26196..26882
                     /locus_tag="U2M88_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM8373110.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            26857..28205
                     /locus_tag="U2M88_000708"
                     /pseudo
     CDS             26857..28205
                     /locus_tag="U2M88_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            28196..28669
                     /locus_tag="U2M88_000709"
     CDS             28196..28669
                     /locus_tag="U2M88_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEM8373111.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            28644..29171
                     /locus_tag="U2M88_000710"
     CDS             28644..29171
                     /locus_tag="U2M88_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HEM8373112.1"
                     /translation="MKIKRRMINMTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATK
                     SGAEFIDIDLIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSY
                     NGFDELLADFVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGP
                     ERSRATTVRNDNLPF"
     gene            29186..31498
                     /locus_tag="U2M88_000711"
     CDS             29186..31498
                     /locus_tag="U2M88_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEM8373113.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMNGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            31745..32062
                     /locus_tag="U2M88_000712"
     CDS             31745..32062
                     /locus_tag="U2M88_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEM8373114.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            32059..32220
                     /locus_tag="U2M88_000713"
     CDS             32059..32220
                     /locus_tag="U2M88_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEM8373115.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            32217..32543
                     /locus_tag="U2M88_000714"
     CDS             32217..32543
                     /locus_tag="U2M88_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373116.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            32540..33355
                     /locus_tag="U2M88_000715"
     CDS             32540..33355
                     /locus_tag="U2M88_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HEM8373117.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            33494..33805
                     /locus_tag="U2M88_000716"
     CDS             33494..33805
                     /locus_tag="U2M88_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEM8373118.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            33802..34032
                     /locus_tag="U2M88_000717"
     CDS             33802..34032
                     /locus_tag="U2M88_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373119.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDNTFRLGS"
     gene            34039..34242
                     /locus_tag="U2M88_000718"
     CDS             34039..34242
                     /locus_tag="U2M88_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HEM8373120.1"
                     /translation="MNTRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILNDDRKWEEKE"
     gene            34239..34538
                     /locus_tag="U2M88_000719"
     CDS             34239..34538
                     /locus_tag="U2M88_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373121.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVINNEV"
     gene            34528..34692
                     /locus_tag="U2M88_000720"
     CDS             34528..34692
                     /locus_tag="U2M88_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373122.1"
                     /translation="MKFKIFEEDTRYKLEKELNDFAKNNEIQHISLATSKRGYANYYA
                     AVVSYVSREV"
     gene            34781..35194
                     /locus_tag="U2M88_000721"
     CDS             34781..35194
                     /locus_tag="U2M88_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEM8373123.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            35207..35593
                     /locus_tag="U2M88_000722"
     CDS             35207..35593
                     /locus_tag="U2M88_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373124.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            36559..37104
                     /locus_tag="U2M88_000723"
     CDS             36559..37104
                     /locus_tag="U2M88_000723"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8373125.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            37105..37422
                     /locus_tag="U2M88_000724"
     CDS             37105..37422
                     /locus_tag="U2M88_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEM8373126.1"
                     /translation="MKEVRPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(37579..37743)
                     /locus_tag="U2M88_000725"
     CDS             complement(37579..37743)
                     /locus_tag="U2M88_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HEM8373127.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            37845..38366
                     /locus_tag="U2M88_000726"
     CDS             37845..38366
                     /locus_tag="U2M88_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEM8373128.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            38353..38826
                     /locus_tag="U2M88_000727"
     CDS             38353..38826
                     /locus_tag="U2M88_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEM8373129.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKFCRLKIGQNR"
     gene            complement(38833..38991)
                     /locus_tag="U2M88_000728"
     CDS             complement(38833..38991)
                     /locus_tag="U2M88_000728"
                     /inference="COORDINATES: protein motif:HMM:NF024064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEM8373130.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            39084..39845
                     /locus_tag="U2M88_000729"
     CDS             39084..39845
                     /locus_tag="U2M88_000729"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEM8373131.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            40065..41384
                     /locus_tag="U2M88_000730"
     CDS             40065..41384
                     /locus_tag="U2M88_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEM8373132.1"
                     /translation="MVAYDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFG
                     KPKRSSINYAPAFSKMEPRASDSRKLDGLNTHFGIFDEIHEFTNYNLINVIKKSRGTR
                     KQPLIVYITTAGYVLDGPLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPR
                     LWIKANPNICLMNFVGMLDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKR
                     NNKTIDIETLKGKKCVGGFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDN
                     NQERIKAWEKVGDLTIIPGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELE
                     NAGFETNEIRQGFLSLGGPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWM
                     PSKQSKSRKIDGFAASLNSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            41400..42620
                     /locus_tag="U2M88_000731"
     CDS             41400..42620
                     /locus_tag="U2M88_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEM8373133.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            42655..43332
                     /locus_tag="U2M88_000732"
     CDS             42655..43332
                     /locus_tag="U2M88_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEM8373134.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            43329..44522
                     /locus_tag="U2M88_000733"
     CDS             43329..44522
                     /locus_tag="U2M88_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEM8373135.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            44537..44863
                     /locus_tag="U2M88_000734"
     CDS             44537..44863
                     /locus_tag="U2M88_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEM8373136.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            44867..45202
                     /locus_tag="U2M88_000735"
     CDS             44867..45202
                     /locus_tag="U2M88_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEM8373137.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            45199..45594
                     /locus_tag="U2M88_000736"
     CDS             45199..45594
                     /locus_tag="U2M88_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373138.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            45591..45962
                     /locus_tag="U2M88_000737"
     CDS             45591..45962
                     /locus_tag="U2M88_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373139.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            45978..46538
                     /locus_tag="U2M88_000738"
     CDS             45978..46538
                     /locus_tag="U2M88_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEM8373140.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            46663..46974
                     /locus_tag="U2M88_000739"
     CDS             46663..46974
                     /locus_tag="U2M88_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373141.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            46998..47189
                     /locus_tag="U2M88_000740"
     CDS             46998..47189
                     /locus_tag="U2M88_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373142.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            47189..51037
                     /locus_tag="U2M88_000741"
     CDS             47189..51037
                     /locus_tag="U2M88_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEM8373143.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            51038..51910
                     /locus_tag="U2M88_000742"
     CDS             51038..51910
                     /locus_tag="U2M88_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HEM8373144.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            51907..52956
                     /locus_tag="U2M88_000743"
     CDS             51907..52956
                     /locus_tag="U2M88_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373145.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            52960..53784
                     /locus_tag="U2M88_000744"
     CDS             52960..53784
                     /locus_tag="U2M88_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373146.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            53802..55862
                     /locus_tag="U2M88_000745"
     CDS             53802..55862
                     /locus_tag="U2M88_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEM8373147.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            55879..56028
                     /locus_tag="U2M88_000746"
     CDS             55879..56028
                     /locus_tag="U2M88_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373148.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            56025..56471
                     /locus_tag="U2M88_000747"
     CDS             56025..56471
                     /locus_tag="U2M88_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373149.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            56473..56610
                     /locus_tag="U2M88_000748"
     CDS             56473..56610
                     /locus_tag="U2M88_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEM8373150.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            56645..56887
                     /locus_tag="U2M88_000749"
     CDS             56645..56887
                     /locus_tag="U2M88_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HEM8373151.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            56903..57100
                     /locus_tag="U2M88_000750"
     CDS             56903..57100
                     /locus_tag="U2M88_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEM8373152.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            57111..58127
                     /locus_tag="U2M88_000751"
     CDS             57111..58127
                     /locus_tag="U2M88_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEM8373153.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            58242..59450
                     /locus_tag="U2M88_000752"
     CDS             58242..59450
                     /locus_tag="U2M88_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373154.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
     gene            59434..59604
                     /locus_tag="U2M88_000753"
     CDS             59434..59604
                     /locus_tag="U2M88_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373155.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
     gene            59859..59932
                     /locus_tag="U2M88_000754"
     tRNA            59859..59932
                     /locus_tag="U2M88_000754"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59893..59895,aa:Ile,seq:cat)
BASE COUNT        20726 a        10141 c        12618 g        17048 t
ORIGIN      
        1 aggggcggta cataaaaatt tattatttag attctataaa catgtgcaac ttatctaaca
       61 acaaagtata tattctacag aagtaaacaa atagcttata ttttaggatg tacacaaatt
      121 gacaagtata ataaaacttt gataaaatac aaatgttttt tatttttatg tgtgattagt
      181 tagaacttac ttaaatccta ttataatatt tttgttaatg attttaataa aatgattgga
      241 gagaaaatga tggatcaaaa agcgtttatc gaatctattg attctacaat cgatagatta
      301 aaaaaagaaa ttcgatttta taatagagta attggtatcg gaaatattat aaaaatagtc
      361 ttatcagcta gtatcccaat attaattgat caggcttccg aacacaggtc tttactattg
      421 attgtttcaa ttgcttcagc aataattaca attatacaaa gtgggatgtc tgcatttaat
      481 tatcaagata aagtacagac ttcgacagag ttattaatga aaattgaaaa agaaaagttg
      541 ttatatataa cgaaaacatc cccatataat aaaacagatg aagaaaattt ccatttaatc
      601 gttacaaccc ttcaaactga attaaatgat attatctctg attttaatca agttaataac
      661 taaaaattta tttaattaga aagtagtgca gataatacta attgtacaat taagaaaaga
      721 gataagactt tacattccta aaaaaatgta aagtcttatc tcttttctta atgctataaa
      781 ttcataataa cattatggac aataaattta aaatttattc agactgtaga caaactccta
      841 aatttagagt gttgtttgca gtctgacgac tcaaaaatga gtcgtataaa tcaattatag
      901 taataagtca ttgatttctt taaattcttt gtcaagttct ttttcatcat atttttcata
      961 tttatcagct tcatgaagaa cttttttaat ttcatgaata gcctttcgtt caactaacca
     1021 tttttttagg ttatgttttt tttctggatt ttcagataac gtatcaagtt cgtctaactc
     1081 tttatccagt ttattgacaa cagaaacaat tttgtttaca actttttctt ctttgttttc
     1141 taattttgac atttatcttc acgtcctttt tatttgataa tttaagtata gaactttgat
     1201 atagtttata caaataaaaa cgcttctttg tgtattctgt gcttttcttt tggaatgagg
     1261 aaaactttgg agtaatatga acgtggacag aaaaatattt aggaggaata aactatgtgt
     1321 acgtctatta cttatgtaac aagtgatcat tattttggaa ggaattttga ttatgaaata
     1381 tcttacaatg aagtagtcac tgttactcca agaaattata agttgaattt tcgaaaggta
     1441 aatgatttgg atactcatta tgcaatgatt ggtattgccg ctggtatagc tgactaccct
     1501 ctttattacg atgcgacaaa tgaaaaagga ttgagtatgg ctgggctaaa tttttctggg
     1561 tatgctgatt ataaagaaat acaagaaggg aaagacaatg tatctccttt tgaatttatt
     1621 ccttggattt taggacaatg ctcaacagta ggagaagcta aaaaattgtt aaaaaatatc
     1681 aatttagcaa atataaatta tagtgatgaa cttcctttat cccctttaca ttggctatta
     1741 gctgataaag aaaaatcaat tgtcattgaa agtatgaaag atggacttca tatatatgat
     1801 aaccctgtgg gcgttcttac caataatcct tcatttgact atcaattatt taatttaaac
     1861 aattatcgtg tcttatcgag tgaaactcct aaaaataatt tttcaaatca aataagtttg
     1921 aatgcctata gccgcggtat gggagggata ggcttgcctg gagatttatc ctcagtatct
     1981 cgttttgtta aagcgacttt tacgaagctg aattctgtat ctggagattc agagtcagaa
     2041 agtattagtc aatttttcca tatcttaggt tcagtagaac aacaaaaagg tttgtgtgat
     2101 gttggtgatg gaaaatatga atatacaatt tattcttctt gttgcaatgt tgacaaagga
     2161 atctattatt atcgaacata tgaagacagt caaattactg caattgatat gaataaagaa
     2221 gacttagata gtcataagtt aattagttat ccaattatag aaaaacaaca aattaaatat
     2281 ataaattagt taatgtgttg tgattgatta tttaatatag atataataca aaaaggcaaa
     2341 gatattttca aaacattttt tcttgatgtg atatccttgt cttttttgta atgtttttaa
     2401 cttattgctt ttgcaaaatt tatttacctg ttttctggat caacgatagt gtatattatg
     2461 ctatccttgt ttggatttta ggacgtttaa agaggcactt ttttacataa cttaatccat
     2521 agacctttta agcatgctgt gagataataa taaagaagag tttaaagcgc accccaaacc
     2581 acttccccat aagtgtatta cgctttaaac tcttttatat ttgaattcac taagaagcat
     2641 accatatttt tgaaaaaaag tgagaaaaaa ggcttataat taggatttag agtaattaat
     2701 tagatgttta ttttatagga ctgatattat agattacaaa tagtatcgta tttcgcaatc
     2761 ttaaaattat cttcttacta tgaataagat aataaaattt gtatttgtcc tttttaggta
     2821 acttatttta atctatatac ctttctggat caacgaaagt atactttata tagtcataac
     2881 gccgatggta actatgcact gtaacgataa tgctgctaaa atatcttttt acgaaaaaca
     2941 tcacaaggaa aaacttaact atggtattat ctaaacataa ttttttcggt atttaatatt
     3001 taattaggag ggaatatttt gcttttttat agtaatttta tcttaatagt agcgatatta
     3061 ttacttctga atatatggat ttttgatagg agtagaaatg caagtatagg ttttagaact
     3121 aaacgaagtc tgtcttctaa aaaaaattgg gtatactcac aaactatttt ttatggagga
     3181 attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat agatgtttcc
     3241 acctcaaatt caatatctat tattggaatc attattgctg caattattac acaactgttt
     3301 ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata gttttggtga
     3361 tagtgatatt aaaaatcaga aaagatagaa aaagcaggag atagataaaa tgctgatatt
     3421 tcagcatttt ttaagagtat aaaagagaaa aaaagtaata aatgtataca tgatataaga
     3481 aaaacatata tgtataaaaa gaagaaagta gcttctcttt gtacatatat ggtttgtttt
     3541 tatggatgcc gagctttttt ataagataag gctaccgccc ataacgaccc aaggatttgt
     3601 ggaactaagt aaatacctgc aagaagcaag atcagctggg tcggttttaa aggatgatag
     3661 aagtaacgcc attcagtaaa attggcgcaa aataaatagg aagtgacagt agatataata
     3721 ttggcaatga ataaagatat aatacgcctg ttcctttgaa aataatatcc agcaaaaaga
     3781 gataaaaata tcaatgtgat gaacccgctt acggaatgat aacgaaaatc aagcagtaag
     3841 gcgaaatgca cataaggaac aaaagataaa agcaacggaa ggtttttcca gcaagttttt
     3901 atcatctttc atcaccacct aacaagtgtt attattttgt tagttttatt gtaaattgaa
     3961 tcctgcaata atcaattact ttttcattat ttctagatta aatgaataaa ctctcatgcc
     4021 attatctcta aaaaaaaagt ttttccccct aattttttta gatagaaaat tgtatagtat
     4081 gatgagagaa aaagtagttt gggggaacgt taatgagaag aaaagcagta atcgatcggc
     4141 tttatttatt tttttggttt tttcctatcg ttttgctacc ttttacatgg gtaaatgctg
     4201 ttgtacaagg tgcggagata ttaggcctga tccaaggaag cggatttgtg gtttctagga
     4261 attttccagc tttgactgtt ttctatttag tgataagtgt aggtctctta ttaccaaaat
     4321 ctctgatcaa tcactacaat tggcagctgc tttacttttc ttgcttatta gtatttacat
     4381 ctatgttttc tttgctgcct cgcttgatta tcggccctaa tggtttgagc gagagtggat
     4441 tgaaggattc agcttattcc tccatgtatt tcttgacgat caaaccatgg ttctatttag
     4501 taattgcctc atttattctt gcaacatttt tttacttctg gacagaaatc aggataaaaa
     4561 gtgaacgatc tgtatctata aaaaataact attgatggtg gatcatcagt aaaaaagcat
     4621 atgagctgcg taaaatgcat gtttctcata tgcttttttt attattatgt aaagaatgcg
     4681 aaaaacgttt acatgatttt tacataactt aactatattt ttacagcaac catacgaggg
     4741 atttacagtc gactgataaa ctttagttgt aaataagaaa ggagcaataa aatgaaaaaa
     4801 gtattgttta ctctaggtat gatgggaatt cttttagccg gatgcggctc aggatcagga
     4861 aatacagatg gctctgccac acaaaataat tcaagtgact caaatgaaca agtaaaaatc
     4921 gttgcggtag gttcgacagc attacagccg ttagtcgatg cagcacaaga aagctttgta
     4981 caagaaaatc caaattatca aatctctgta caaggaggag gaagcggaac tggtttgagc
     5041 caagtcgaag ctggagcagt tactatcgga aactctgatg tatttgcaga agaaagagac
     5101 ggagttgatg cttcaaaatt agtggatcat aaagtagcag tggtcgggat ggcgccaatc
     5161 gtcaataaag atacagatgt caaagatatc accaaacagg aattgattga tatctttaca
     5221 ggaaaaatca ctaactggaa ggaagttggc gggaaagatc aaaaaatcaa cgtcgtgaac
     5281 cgtgcaaacg gcagcggtac ccgcgcgaca ttcgaaaaat ggggactgga tggtgctacc
     5341 cctgtgcagt cccaagaaca agattcatca ggaacagttc ggcagctggt aagtcaaaca
     5401 ccaggagcaa tcagctattt agctttctca tatctcgatg attccacaca agcactaagt
     5461 atcgacggtg tagaaccaaa agaagagaat gtagcagata acagctgggg aatctggtca
     5521 tatgaacata tgtacacgaa cggaaaacct tcccctgaag tccaaaaatt cttagactac
     5581 atgatgacag aagaaatcca agaaggacct gtcaaagaat tgggctattt accaatcaca
     5641 atgatggaag tagaacgtga tcacgaagga aatatcaaat aactagcaga taaacatgcc
     5701 acggtagtcg ttactattaa ggaaaaatca actatgtttc ccacatatac atttttcctt
     5761 taattgtgaa aaatagttcc tcatattttg ccgttttttg agctaatacc gagggtcagc
     5821 tgattaaaca ccgtttattc gaaaaaaaga gctacgacat agaactgtcg tagctctttt
     5881 tttgctatat gacaggaatg aaaggtgttt gcaagcttaa tgtgaggaac actttccata
     5941 tctgttctat actggatagg agaagtttgg aaatatccag aaaacgaaat caatcgaggt
     6001 agccacatga agaataaatg gataaatcga agtaaaaaaa agaaagtaac acaaagatca
     6061 tcagataaag tcatccgtct ttttccatcg tataaagaag gattaacggc agatcaagtc
     6121 aaggaacgaa tagaaaaagg tgcagcaaat aattcagtcg atcccacatt caaaacaaat
     6181 cagcaaatcg tattggaaaa catatttacg tatttcaatt tgatctttct tatcttagcc
     6241 attttgcttt gtttagtaga atcctataag aacttgactt ttttaccagt tatcatagcc
     6301 aatacaggaa tcgccatttt tcaggaaatt cgatccaaaa agatcttgga cgaactaaat
     6361 gttttaaata cagcaaaagt caatgtcata cgggacggga tagaacaaac aatagatata
     6421 gaagaccttg taatagatga cattgttatt ttagagactg gacatcaaat tccagcagat
     6481 gcagaggtcg tggaaggaaa actgcaagtt aatgaggctt tattgacagg cgaagcagat
     6541 gaaataacca aagaaatagg cgatccatta atgtccggca gtttcattgt ttccggaaaa
     6601 gcttacgccc gattagacaa agttggagca gattcgtata tttcccagct gacagtcaaa
     6661 gccaagacaa tgggagaagg agaacagtcg gagatgattg cttctcttaa tcaattgatc
     6721 aaatggattg ggatcatcat tattcctatc ggtatcctgc ttttttctca aagttacttt
     6781 tttaatggaa acacgatcaa agaaagtgtc gttgcaatgg aagcagcatt gatcggtatg
     6841 atacctgaag gactgtactt gctgacgacg attgcattag cattaagtgc gactaaacta
     6901 gcaaagcaaa gagttctttt acacaatatg aaaagcattg agacattggc aagggtaaat
     6961 gtcctttgtg tagataaaac aggaacgatc acagaaaata aaatgtctgt tcaaaaagtg
     7021 atcgtctcta aaaaacaaga aataatcagt cagtcagatc aattggaagc atgcattgct
     7081 gactatgcaa aagcgatggc aggagacaat gcaacaatgg aagctgttaa agcatatttt
     7141 acaaaaacca ctgatcaatc ctatcaccat gttattcctt tttcttctgt ccaaaaattc
     7201 agcagtgtag cgttggcaga caaggtgtat gttttaggag cgcccgaaat ggttttgcgc
     7261 gatcaattgc cggaatatgc ggaagagttt gtttctttcg ctgaaaaagg ctatcgcgtg
     7321 ttggtctttg gtgtgtacga tgggattctt gaagaaccag tattgacaga agcagtcaca
     7381 ccattaggct atattctgat tgctaatcct atcagaaaag aagcaagagc cacatttgat
     7441 tacttcaaaa aacagcaagt agccatcaaa gtgatttctg gagataatcc gctcaccgta
     7501 tccaatgtag ctgtccaagc agggatttca catgctgatc agtacgttga tgcatctaaa
     7561 ttatcagaag aagaatatga agcagctgtc gaaaaatata cagtttttgg acgcgtcaag
     7621 ccagaacaaa agaaaatctt cgttcaattg ataaagcgta acaatacagt agctatgaca
     7681 ggtgatggcg tgaatgatat tctagccatg aaagaagcag actgcagtat agcgatggct
     7741 tccggaaatg aagccgccat gcaggcctca caagtggtct tacttgattc tgacttttca
     7801 cgcatgccag aagttgttgc agaaggacgg cgcgttgtga acaatattga gcgatcagcc
     7861 agcttgtttt tagtgaagaa tattttctcc tttttgctct cactgttttc agtgatcttt
     7921 gctctgacct atcctttaga accatcacaa atcacattga ttagtttgtt tactatcgga
     7981 cttccatctt tcttgcttgc tctagaagaa aacaaaaagc gaatcagagg gaaattcatc
     8041 atgaatgtga tggaaaaagc tgtgccaggt ggactaacag atatgatcgt agtcggtgcg
     8101 ttggtgatct gcggggtgac tttgaatcta aataaaacag atgtttctac agcatctacc
     8161 atgcttttga ttgcagtagg gttcctcgtt ctctataaaa tctgttcacc gctgaataaa
     8221 ttcagaagtc agatcattct tttctgtgct agcggtaact ttttttcggt catctttttg
     8281 cataagctgt tttcaatcac cggtatctca gctgtatcac ttttgctgtt gagcattcta
     8341 tttttctcgg cagattcgat ttttaggcat ttgaccacat tggtagaata tttgtttgaa
     8401 cgtaaaaatg aaaaagagca aaagccgttt agtcgcgtag ctaaaaaatt tctatcgata
     8461 tttaaacgag aaaattgacg aaaagtatct caaaaaagag taaaaaagaa caaccactcg
     8521 tgtatctttc gaagcaggat gagtcatcca actttaaaac gcacgagcag ttgctctttt
     8581 ttacattttc ggaatggtaa cggtgaaagt cgaacctgtt ccaagatgac tgtctacagt
     8641 aatcgtacca ttaaggagtt gcacgtagtc ttttacaatc gctaacccta atccggtccc
     8701 gcctgaatgt cggcttcgcg ccttgtctac gcggtaaaaa cgctcaaaga tccgttcctg
     8761 atcctcaatg tcgataccta taccaaagtc ttggacagaa aacgatagat catctgttgc
     8821 ctgatagcgg ataataatct cgctgtcgct ttttgaatat tggacagcat tttcaatcag
     8881 atttttgata attggataaa ggatatctgt tttagtagta aatggttcgt tctttccaat
     8941 caatcgaatc gtcaaccgtt tcttttcgat gattggctga taattttgaa cgatttgctg
     9001 gaaataaggt tctgtataaa ttgtctggat ctcgtaagaa ttctcgccat cttttgacag
     9061 ctggatgatt tcccgaatca gtttatctaa ccggatcgca tctttttgca tgatctcaag
     9121 aaaagaagtc agagtttgcg ggtcatcttt tgcaccatcc agtaaagtct cggtaaaacc
     9181 aatcagtgaa gtcacaggag tttttaattc gtgagagacg ttgccaacga aatccttttg
     9241 tagcttttct aaacgtcgca ctttcgttaa gtcatacgcc acccctagaa tctgtccggt
     9301 accttctgaa ttagtaaagt agcgcaaact caagtctaat gtctgccgac cttctgtaag
     9361 ggtgatctcc tgatggatca gcggatgctc aggagtcact tgatgaatca gctgaatgag
     9421 ttttggctct tgaatgactt ctgtgtaccg ctgttcaggt tgatagctta ccacgtttaa
     9481 atgtagctgc atctttggat taagcagtag taaacgagaa tcagcatcat caatcaggaa
     9541 tacgccaatc atcagttcgt tcagtaacgt atataattgc tcttctgtgg tagtacacgc
     9601 ccgatacatc ttactcattt gttcgctcaa cgcattaatc gtctgataca attcttccca
     9661 ttgatcgggt gtttgcatga ttacttccgc ctgattagga ttttgcacca ttttttttag
     9721 tacgggcagc acggttttca atggctggtt tttttgatag atcagataat agatcattcc
     9781 tgcaagaaca aggaagaaag acagaaagaa gaaaaagacc caacggcgaa aactctcagt
     9841 tctcggtaaa aaaccgcttg tcggttctgc gatacgtaaa atggcttcta actggccact
     9901 ttttttgact ggtaatgcga catataaaag atcctcattc aaagtagtac ttttgcgcaa
     9961 agcgctacct aggttcccac cattcaatac ggctttgatt tcgggacggg agtttcgctg
    10021 ttcattcaat gtttcattgt ttgtgtcaaa taagatcttt ccagtagcat ccattaaagt
    10081 aatccgttcg ttcgactgat ggacgaattc ttccaaagca gttttgtttt gcgctgcttg
    10141 gaggttttcg acatctaatt gccggatcag cagttgtgct ttttttgtca ggtagctttc
    10201 ttgctgttcc acgatctgct gttgaaaata atgggaaatc aattgccagc caccaaaaaa
    10261 caaactaact aaaagaataa gaaagaagag acgttcataa atttttttcc atttcatcgt
    10321 tttggttcct gaaacttata gccaaatcct ctaatagtca ccaagtactt tggatgtttt
    10381 ggatctcgct cgattttttc tcgcaaatga ctaacgtgta catctacgat tcggctttgt
    10441 cctgcgaagt caaaattcca aatgcggtct agcaatgtgt ctcggtcgat cacacggtct
    10501 tttcgtttca tgaaataaac aagtaattca aattctttcg gtgttagttc aatcagatgg
    10561 tcgtctacgg tcacttgata attggtcaaa tcagcagtga tttgtccaat cgatagatat
    10621 tcaggctcag cttcatcttt tgattgctta cgaggttcaa tccgtctgaa tattgctttc
    10681 atacgggcta atacttcgcg aggactaaat ggtttggtca aatagtcgtc agcacctatt
    10741 tctaggccaa tgatccgatc cacttggtca tctttagccg tcagcatcaa aatcggtgtt
    10801 tcattttttt cttgacgcag ccgctgagtg atctccatgc catccatcgt tggaagcatc
    10861 acatctaaaa tgatgaaatc aaatgattgt tctaatgcta attctaatcc ttcgcctcca
    10921 tctgtggcac tggttacctt gtagccttct ttttccaaat tgaacgtcaa gagcgttaca
    10981 atagaaggtt catcatcaac aactagtatt tttttcattc aaatctcctt taaataggtt
    11041 tcattctgtt tatattttag catagaatca gtacaaaggg ctattttaca aaagaaataa
    11101 atggtataat aggaaggcac tttgctgaat agaaaggaag agcagtcaat gattggaatc
    11161 agtgcatgtt taggcggtct ggcctgtcgc tatgatggac agaaaaaaga agtaccagaa
    11221 ttgatggaac tagttgaaac tggagaagct gtcatggtct gtccagaagt gatcggcgga
    11281 ttaccaattc ctagagatcc cgctgaaatc attggtggaa atggattcga tgtgtggaaa
    11341 gatcaagcaa aagtatggac gacaagcggc gaagatgtaa cggaagcgta caaaaatggc
    11401 gcgatcaaag cctatcaaaa attgcaggaa aaacacattg atctagtcat catgaaggaa
    11461 aaaagccctt catgcggaac ccattcgatt tatgacggaa gtttttccgg aataaaaaaa
    11521 aatggtgccg gagtggctac tgcttatttt attcagcaaa aaatgactgt gctctctgat
    11581 gaagaatggc tgaaaactag aggtgagcag aatggaaatc gaaaagacca atcgaatgaa
    11641 cgcgctcttt gaattttatt cgaccttatt gacagaaaaa caaatgaact acatggaatt
    11701 atattatgca gatgattttt cattaggtga gatcgcggaa gaatacgaag taagccgaca
    11761 agcagtatat gacaatatca agcggacaag caagatcttg gaggattatg aaaaaaaact
    11821 ccatcttttt tctaattata ttgttcgaga acaggtattg gaaaatatga ctgcatatat
    11881 tacagataag tacccggaag ataaaaaact acttgaatat gtacagcaaa ttcaagcaat
    11941 cgaagaataa ataaaaggaa tgaagaaaat atggcatttg aaagcttaac tgaacgtctc
    12001 cagcaggcga tgggaaaaat aagaaaaaaa ggaaaagtat cagaagctga cgtaaaagaa
    12061 atgatgcggg aaatccgcct tgccttacta gaagccgatg ttaacttgca agtagtcaaa
    12121 gattttacta agcgcgtacg cgaacgtgcg gtaggggcag aagtgctgga aagtttatca
    12181 cctgcacaac aaatcgtaaa aatcgttgat gaagaattga cgatcacact tggttctgaa
    12241 acagcagagt taaataaatc accaaaaata ccaactgtta tcatgatggc tggtcttcaa
    12301 ggggcaggta aaacgacctt tactggtaaa ttagccaatt atttaaagaa aaatgaaaat
    12361 gctcgtccgc tcctaatcgc aggggacgta tatcgaccag ctgcgatcga ccaattgaaa
    12421 gtattaggtc aacagctaga cgttcctgta tttgatatgg gaactgaagt cagcccggta
    12481 gagatcgtcc gtcaagggat ggagttagca aaagaaaaga aaaacgacta tgtattgatc
    12541 gatacggcag gacgtctgca tatcgatgaa acattgatgg atgagttgaa acaaatcaaa
    12601 gaattgacgc aaccaaatga gattttgctt gttgtcgatg ccatgactgg tcaagatgcc
    12661 gtcaacgtag ctgatagctt caaccagcaa ttaggtatta caggggtcgt catcactaaa
    12721 cttgatgggg atactcgagg cggggcagca ctatcgatcc gctcagtgac aggcgcacca
    12781 atcaaattca tcggttctgg tgaaaaattg actgacttag aagtattcca cccagatcgt
    12841 atggctagcc gtatccttgg tatgggggat atgctgacat tgattgaaaa agcgcagcaa
    12901 gactacgacg agaaaaaagc ggaagaactt gcacagaaaa tgcgggaaaa ttcattcgat
    12961 ttcaatgact ttattgaaca gcttgatcaa gtgatgggta tgggcccact ggaagatcta
    13021 atcaaaatga tcccaggaat gaatcaagtc cctggaatcg aaaacgtcaa ggtagatcca
    13081 aaagatgttg aacgcaaaaa agcgatggtt tattctatga cgccagcaga aagagcaaat
    13141 cctgatctgt tgaacccaag ccgtcgtcgc cgaattgctg ccggttctgg aaacagtgtt
    13201 gttgaagtga atcgaatgat caaacaattc aaggaatcaa gaaaaatgat gcagcaaatg
    13261 tccaaagggg atatgaacat cccaggtatg gaccaaatgt ttggttcagg cgtcaaagga
    13321 aaactaggca aaatggcgat gaatcgaatg atcaagaaaa ataagaagaa aaagaaaaaa
    13381 cggaaataag atgaaaaaaa cagcttcggt acagactgaa aatgtctact gccggagctt
    13441 ttctatcagc aatttttatt ctttactaat acaattactc taaacgaaga ggtggcacag
    13501 atgagatgtc cgtggagtga acaaacagaa agtatgcggc aatatcatga tactgtctgg
    13561 ggtgttcctg aatatgatga ccaaaagctg ttccgaaaat tgatgcttga tattaatcaa
    13621 gccggattga gctggcagac gattttaaat aaaagtgaag cattcgatga agcatatgat
    13681 cgattcgata tcaaaaaagt cgcaaattat ggagaaacaa aaatcagtga actaatggaa
    13741 aacaaaggaa ttattcgtaa ccgaagaaaa atcgaagcag ccgtccaaaa cgccaaagca
    13801 gtcttgaaaa ttcaagaaga aatcggcagt ttttcagagt atctttggtc gttttctaat
    13861 ggaaaaatcg ttgattcttc ttatcattca caagaagaag tcccaacaac aagtcctctt
    13921 tcagatgaat tagcaaaaga tctaaagaaa agaggattta aatttatcgg aagcacaaca
    13981 atctatgctt ttttagaagc tgtcgggatc atcaatgatc accttgacag ttgtttccgc
    14041 aaagaagagg tcgtacgtat cggcagaaag ggcgaataaa gcatgcgaaa aatcatattt
    14101 tatggtgcta taagtttaga tggctatttg gcaacgaaaa cggatgatct tcaatggctt
    14161 tttgacactc caacaggtaa gcaaactact tatgaagctt tttatgaaac aatcgatacg
    14221 accatcatgg gcagaaaaac ttatcaggaa gcaaaaaaat atttggagac cgaagcaatt
    14281 tatccagaga aaaagaattt cgttttttcc acccacgcac aattacaatt gccagatgct
    14341 gaggtcgtaa acgaagatcc tgttgctttt ctaaaaaatc tacaaatgac agaaggaaaa
    14401 gatatctggg ttgtgggtgg cggtcgattg atcaaaccgc tattggaaca tcaaatgatt
    14461 gatgaatggt atatccagat tacccctgta ttattagggg acgggattcc tttattccaa
    14521 aaaggaacat atgaagcgcg ttttgaaatg ctagacacga cacgattcgg tgaatttatc
    14581 gaattgcatt atgtacgaaa gtagaagtaa ataaaataaa aaaggcaact atatgcagaa
    14641 atgtcctgta catagttgcc ttttttatta ccgttatgct ttttactctg ttaatttgtt
    14701 agaaaaatct agatacgcat ctaaaatcgt ttggaagaaa cttaatgttc cttcaacgat
    14761 attaccattt tcatcaatta gattcgcaac gtttccgata tatgcttccg gttgctgcaa
    14821 ggttggcaca ttcaaaaaga caagtgattg gcgtagatgg tggtttgcgc caaagccgcc
    14881 gatcccacct ggtgatgcag taacgactaa acctggttta tgatcccaaa cactgtgtcc
    14941 ataaggtctt gagcctacat ctaaagcatt tttcaatact gctggcacag aacggttgta
    15001 ttcaggagta acgaaaacga caccgtctag tcccctcact tcctcacgaa aacgagtcca
    15061 ttcagcagga gcctgttctg gagtttcgag atcctcattg taaaatggca gatcatcgat
    15121 ttttacaaat acactttcgt acccttcagg aagcaatttc gcaaaagctt ctgctacttt
    15181 tttgttgtaa gaatcttttc ttaaactacc aataaaaaat ccaatttttt ttgtcatacg
    15241 atttttcctc cttcaaacta tctataataa tcttacaaaa gataagtaac tggagcaaat
    15301 aaaaacactt actgacaaaa gctacataga agatagatta cttattttta acaaaaaaga
    15361 agtctaaaat cttctatttt ctaaaaaaaa tcgaatttaa tcaagctgta aagaaaaaaa
    15421 gctttacagg gtaaaaaaaa actgttacac tacatcttgt gaataaaaca atggaggtgg
    15481 aaataaataa tggcagttaa aatccgttta aaacgtatgg gttctaaaaa gagtccgttt
    15541 taccgtatcg tcgtagctga ttcacgttct cctcgtgatg gacgtttcat cgaaactgta
    15601 ggtacttaca atcctttgaa agaccctgca gaagtagttt taaaagaaga cttagttctt
    15661 gactggttat caaaaggtgc acaaccttct gacactgtac gtaacatcct ttcaaaagaa
    15721 ggcgttatgc aaaaacacca cgaagctaaa ttctcaaaga aataaggtga ccggatatga
    15781 aagatttaag cgacttagtc ttaacaatcg ttcgtccgct agtcacttat cctgatcaag
    15841 tcaagttaga cgtcgtagag tcgaaagact tttacgagta caatctaaca gtggctcctg
    15901 aagatattgg ccgtatcatt ggaaaacaag ggcgtgttgc taaagcaatc cgcacaatcg
    15961 tttacggtgt tcggatcaat gcaccgaaaa aagtgcgttt gaatatcatc gatgataagg
    16021 aaaagtaaat aaacgtgctg tactcgaatt ttcgagtgca gcttttttgt tttttttatt
    16081 aattgaagaa atgattattt ggcaaaacaa gcaagaaagc gtatccttat aggaggaggg
    16141 agtgaacagc caatgagaaa gaaaatgtta tttattccat ttgctctatt gttaagcgct
    16201 tgtagttcgg ttactcaaga atcagacaat acacatctat caagcagtat atctgaaact
    16261 gttaccagtc cagaaaaaag tagtaccaaa acggcttcca ccactgaaca aacaatggct
    16321 acctcaaata atgagaaaaa gactgcttta gaccagttga aagaacaaca gccaaatgtt
    16381 cccatgcctc tagatgtacc agtctcttca ggctatctca atatcgcagc gacacataca
    16441 aagcaaggtt attctatttt atattacaga acagatcgtc ccctcgggtt gaatgctgac
    16501 gaattgaatc aagaaacgcc tattgcaacc tatctatatc aatatgggtt tgcttctagt
    16561 caagaaacaa tccaagtttt gcagccattt gagatcgaca caaatggtca gcaagtggac
    16621 ttaggttctc gaatcacagg gtatcagcaa ggtgcagcag gttcaagttt tcttgaatgg
    16681 caagaaggaa actggcgtat tcgaatccgt gggaataata tagaaggcca agatccctta
    16741 ctattagcta aagaaatcgt agcttatttg gaagaaaaca gtctaccagc acctgaacaa
    16801 tttggtaaaa ttactgttga tatgggtgat acgacgaatc gagccgtaga agtaagctgg
    16861 caagaaccga aaaatgccta tacgatcact catcaagatc ctatgtctgc gttgaagatg
    16921 gcagtttcta tgaagaggtt gtgaaatcag cgcgctgatc tgagcccatc atttaactcc
    16981 aagtcacagg aagtaatcaa aaatagcacg ccgcattttt gcataatttt ggcttaatac
    17041 cttaaaagaa aactagaatt gcctcttcac acatgctaaa ataaaacaga caaaaaagaa
    17101 atggggatat aagttgactg aatacttgaa tgtaggaaaa atcgtaaaca cacaaggtat
    17161 caaaggcgaa gtccgtgtta tctcaacaac ggatttccca gaagaacgtt acaaaaaagg
    17221 aacggtgcta acactattcc aagacggaaa agcacctgtg gaattaacag tcaaaagtca
    17281 tcgcaaacat aaaaattttg acttattgag ttttgaagga catccttcta tcaatgatgt
    17341 ggaaaaatat cgggatggca tattaaaagt gtcaaaagac aactctgtag atctagctga
    17401 aaatgaattc tattaccatg agatcatcgg tctgcatgta ttagaagatg gaactgagtt
    17461 aggtaaagta aaagaaatct tgtcaccagg tgccaatgat gtttgggtcg ttcaacgtcc
    17521 gaaaaaagct gatatattgc ttccatatat cgcttctgtc gtcaaagaag tcgatttaga
    17581 agctggagtg gtccgtgtag aaattccaga aggactgatt gacgatgaag attgatgtat
    17641 tgactttatt tccaagaatg tttgaaggtc ctatggggga atccatcatc ggtaaggctg
    17701 tagataaagg attattagat atcaatatct ctaacttccg cgattattca gataacaaac
    17761 atcagacagt agatgattat ccatacggcg gtggagcagg tatgctgctc aaagtccagc
    17821 ccatctatga taacatccaa gccattgaaa aagctgcacc ggatgtcaaa aaaagagtaa
    17881 ttcttcttga cccagctggt aaacgattcg atcagaaaat ggcagaagaa ttttcacaag
    17941 aagaacactt agtattcatt tgtggtcatt atgagggata tgatgaacgt atccgcacat
    18001 tagtgacaga tgaagtttcc ttaggagatt atgtccttac tgggggagaa ttaggtgcga
    18061 tggtcatgat cgatgcaaca gttcggcttt tgccagatgt gttagggaat cagacttcag
    18121 ctcaaacgga ttcttattca accggtttat tggaacatcc ccaatatact cgcccggcag
    18181 aatataaagg gatgaaagta ccagaagtat tgacaaatgg aaatcataaa ttgattgaag
    18241 aatggcaatt aaaagaatca ctaagaagga cctatcaacg tcggcctgat cttttggaaa
    18301 cacttgaatg gacacctcaa atgacgaaat ttttagaaga aatcaagaaa gaagaacaag
    18361 aaaaaacagc agatgaagcc aagtgacttt ttctaaaatc ttctttacag taggttgata
    18421 atatgttagt attttatggt gagtgcaaaa gcacttaact attacgatat tccgctgtga
    18481 gaaaagctca taagaatatt tggaataagg agaagaaaac catgaatcca ttaatcgaag
    18541 aattaacaaa agaacaatta cgttctgaca tcccagcgtt ccgccctggt gacactgtgc
    18601 gtgttcacgc aaaagttgtt gaaggtactc gtgaacgtat ccagttattt gaaggtgttg
    18661 ttatcaaacg ccgcggtgct ggaatcagcg aaacttatac agtacgtaaa gtttctaacg
    18721 gagtgggcgt tgaacgtaca ttcccattgc atacaccacg tgttgcaaaa atcgaagtag
    18781 ttcgctacgg taaagtacgt cgtgcaaaat tgtactacct acgtgcatta cacggaaaag
    18841 ctgctcgtat caaagaaatc cgtcgttaat catcgttatc agacagagaa agaacccttg
    18901 gtattcaagg gttctttttt tattatctgt ttttttatat taacgatttc tatttggtac
    18961 atttttgatt aataaaaagg ggcaaaaaag gggcaacaag ttacagtttt tctatatctt
    19021 ctttcaattt ttcttttact ccttttgtca catgcaaata aattttttca gtgatatcac
    19081 tattttcgtg accaacgcga tcctgaatgg catacagagg agttcctagt tccgctaatt
    19141 ttgaaatatg ggtatgtcta aaaatatgtg aactaagttt tttatcaatc ttcatgtcag
    19201 ctttatgact tctcaaatag gtgttaattg ctgttagttg gaaaggagtt cctttcgttg
    19261 tttgaaatag aaattgacca tttggattta attctagaag ctcattgtaa atagctatcg
    19321 ctttttttgg taaatcaatt tctcgcattc cggcagctgt ttttgtagaa tcagattttt
    19381 tcatatcagc tattgaacgt tctctataca tcatcgtccc atttataact actgatgcat
    19441 tattattggt aatgtgtaca tcatctttac ttaatgcgat tgcttcacca gacctcatcc
    19501 cagtcaaata tagccactgg aaaaggagag aatatctttt attgtgtgaa gttgtgaaat
    19561 ctactagtct attatattca tcatcttcta aaaatttatc tttaattttt atcgtttttg
    19621 actctctttt ataatcaata actacttcat cgataggatt tttttctaca taaccttttt
    19681 tcatagcata tgaaaaaaga agattcaatt tggatttaat tacactgacg tacttatttg
    19741 acaagtcatc tttatatatc atgtcttcaa aaatattatt caaatctatt gtattaatac
    19801 cagaaacgat gtaagtctca ggtatcttct tctttatagt gtttaatata ttattcgtag
    19861 gataatatgt agattcctta acttgtcttt tgtatataac taaccattct tcaacaagct
    19921 catgaaatgt tagatcaggc ttatgaagtg ttttttcgtt taattttata tcaatttttt
    19981 tatttaactc tagcaatgcc actttctgag tttctcgaga tttattttta taagtaatgc
    20041 tcactttttt tctttttcta gtttttggat caatatatcg ctcattatat ttatatactt
    20101 tttttccgtt tttatcagtt ttagtttcga tccacattgt tttcatctcc tatttgttgc
    20161 tataatagga atagataagt aagccgatta tagcaggttt atttttcatc acgtccacaa
    20221 actttggcga gggaggggcg tgtttttgtt agttattgtg gtaggttatc taaagcgtat
    20281 tgtgcttgct catttgtaaa accttcaaat ataagttgat cgtacaattg cgaatctgac
    20341 attgatgccc aatcatcgta actttccgct ttagccaaag cttgttcgtt gtaatcgaca
    20401 tcaacattag ccagcgcata atcgattgca tcacttggat attcttcaaa ctctagttgt
    20461 tcacgcaaac cttgttcaga cataccagcc caacctagat agctttcagc tttgctcaat
    20521 gcatttctgt attcacgagg aacactttct tcttgagtaa cttcttctat agtagtttca
    20581 gtagtttcag tttcttcttc aacgactgta gattctgcta ccgattctgc agaagacact
    20641 tctgtcacgc tttctttagt tgtttcagcg acggtatttt tagcatcatc actgcctcct
    20701 tgcgaaccta tagcaataaa caccacaaca accgctaaca accaaaacca tacccgttta
    20761 taaaacggtt tcttaacttt atacattttc ccatcttgac ccataacttt ttttgccatt
    20821 taaatatccc tcgcttcttg ttataatata tttgtgatct cagaaatgag gtatgagtcc
    20881 gtgttgcagc acgggctttt ttctttataa cttttttaga gattataggc aaaatagtag
    20941 ggcataaaaa tgtattattg aattccgtat ttagaaaatc ctaattgaac ttcaccggaa
    21001 gtcttttgct gtgttgtacg caatgcttct tcttcagaca ttccattctg tactttccat
    21061 gcaacaggcg acatcccgta tttgttaaca aaatcagtaa gtgataaagt gtcagcgtct
    21121 tgctgagcgc ttgtttgttg gtcttctgga ttctgttgag atgctgcttg ttgttctttc
    21181 tgatcttgac tgataatatt gccagcatca tctgtagtca atccattttc ataaagggcc
    21241 acgccgaaag cttcccactc tttgttggac caatttgcac gatcagctgg agttgactgt
    21301 aaagtgcgtt gtttcatctg ttcatatgtt tcttcttgag gtgcggtttg gattgtactc
    21361 tgactggagc ttataactgt tgggctaggt tccgccgtag cttggttgga gcttgtaact
    21421 gttgaactag aatctgtttt agatgtagat ttgctagtag atgaactggt ttcagttgtt
    21481 tcttttgttt tacttatttt tgtttcttgg ttagaagtgg tatctgttga ttcagctttt
    21541 ttattatttg aacaagctga aagtagcaga gcagtactta acaacaacat aacgctaact
    21601 tttttcattt tataattcct ctttctcgtt gtaatatgtg tgctaacacg ggctttttta
    21661 tataagaaaa cgataagcgc tttctggaag cccgtaaaga ttctttaatt cctcgatttt
    21721 tttaggatat tgatcattat cttctttata aagagaaaca atgagattag cagcaaagca
    21781 attagcttcg ctttcagatt tgcttctaga tgttcttgtt gatacataat agctggataa
    21841 gccacgatga aaaatagcgt gacctaattc gtgagcgcaa atgtagaatc tttcctcaga
    21901 gtctcgcagt tcatcattta aaaagattat cgcacgatct ctaatttctt gaaactgccc
    21961 cttgggattc tcgataaaag gaacgtattg aattttaatg cccatctttt cacaaatata
    22021 aaaaggatta gcggactggt atttccgctt caactcctcg actaaattaa tcgtatccat
    22081 ctccataagc tcacatcttt ttgccttttt ctttgtcttc tttcacaata tcccagaaag
    22141 tcgctatcag gatatctttt acgcgctgta tttgttcggg tgtcaatgtt tccccaccat
    22201 aagacatatt aacatttgag tctagtagtt tatcaagttc aaccacttcc tcttttgtag
    22261 cccacttggg aacattatta tttcccaata aataatcagt tgtgacacca aaataatcag
    22321 caactttctt taagttctca gattttggcg aggctttatc ccatcttctt atttgtccat
    22381 tagagatgcc cacctgtctt tctacttctg ctatagtcac atgcttttcg tctgctaatt
    22441 ctttaatctt agtaactaaa ctcattatta tcaacctttc aaagctgaaa agaaaataaa
    22501 tagcttaaaa gttatatttt tggttgacaa ttagctttta agctagtata tttaattcgt
    22561 aagctaaatt gttagctaaa taagagcaac aaaaaactct actaatttaa aacattctct
    22621 cggtcgccaa acttagaaat gttattttag aggcttttta taagtcttat ttaactatgt
    22681 attcataata gcttaaaagc taataggtgt caacgattta gctaattttt tagcttacaa
    22741 attatttgtt tagaaaggag ctatttttat gtctgagaat ttagacttaa aaattcgagc
    22801 ggagatgaga aaaagaagaa tgactttcaa agaactagct gcgcttatcg gtatttcagg
    22861 agcttattta tcagatattc taaacggcaa tcgtgatgga aagaaagcac aacagcatat
    22921 cgaaacagtg aaaaaaatat tggacatccg ataggggggg taggcgatga caaaactaaa
    22981 aaaacaagat tttgtaaaaa aatataatta ttctccatct acttatcaac gtcgaatgtc
    23041 ggaactaaaa aatacagcaa ttttctcagc ggcgtatgaa cgggtcacag gacaagaagt
    23101 ttggatcaat acagaattat acgataaatt tttgtctttc aaatcctata acaggttacg
    23161 cacaagaaag gtaacaccta aagaatttat cgagaagcat ttagttgatt tataaaaaac
    23221 agaacatttt gagaggtgaa ggttaatggg taaattcaac agagcattag tattcagcgc
    23281 accgctaatc gtctatgctt tagggctttg gggaagtaga caagcgttga taggaacgat
    23341 tgtgtacatg gtttggattt ttatagggct ggatgaagct gagtacaaaa caaaaaagcc
    23401 agtcgggagg gactgactaa tgagtaagga cgatattgta tttctgttaa ttatgttttg
    23461 tgggattgga tcaataataa ttaatttgta tttgattatt aaagaaaaat ttttcaatta
    23521 aaatgagtct tttagcttta acttaaattt tttttgataa ttagctgttc ttatgaccat
    23581 tacaagtttt tcaccaggta atatatcttt catatctaaa acttctgaaa aagcgactga
    23641 gtagccagga gagattgctt gtgccaacgg tataggagat aaaaggctac ttttatgaat
    23701 gcttcccata tcaaaaccat tggcgtgata atgtgaacaa ttaattttct taccgttgta
    23761 aagatacgta cattcgataa ttgagtttgg catttttgat gggtttgcaa ttacgccagt
    23821 aagaatcatt cgattacttc tatcatgctt aactaaaaaa taggagagcg taatttttct
    23881 tttatcttta aataagttat agagggataa aaaaaatcct gaaatagaaa tgattaaagt
    23941 aatagcgact tggtaatcgc tcaaaatttt tatcaagtaa ttcaccacct ttttactcaa
    24001 attatatcaa aaaggagaga agaaataatg caagaattag taattttgaa aaataaaaaa
    24061 agacacgaac gccggcaagc atgatcgtgt cagggaaata aactatcttt ctatatttta
    24121 ccacagaaag gaatgaaccg taaatgctaa ttgcaacgga tacactagac cgcatctttt
    24181 taaaagacga atacaaactg cgcaatatag atgcgtcagg aattttagta tttgatcttt
    24241 atgacaatgg aaaaattggt atctatcaag caagtgatat tgaagaaaca aaccttgctt
    24301 tcgaacaaat tgatgattct gtggaattgg atttagatga ggcaatccta gcttttgaac
    24361 aaattgcaaa attattaaag gaggcacaaa agaatggcaa ctctttacca actcagcgag
    24421 tcatatatca aagtcctgga actggcagaa caattggatg aagaaattct tcgcgatact
    24481 ctcgattcga ttaatgaagc gatcgagtat aaggcagaaa acttagcaaa aatagttaaa
    24541 gaagtagaag ggaaagctga gttaatcgat agtgaaatta aacgtctgca ggaacgtaag
    24601 acatcgcttt tgaacaatgc taagagtatc aagcactatt tacaagagga aatggaaaag
    24661 actggtaaaa cgaagattaa aggtgaatta ttcaacattg gaattcaaaa taatccagta
    24721 tcggtcaatg taatcaatga gaacttaatt ccaaaaggat tctttacccc tgtgcctccc
    24781 aaattggata aaaagcaatt gaaggaggaa ctgaagcacg gagatattcc tggtgctgaa
    24841 ctcgtccaaa caaaaggttt gaggataaga tgatgaagga ggagaaataa tggaacacga
    24901 aaacgtaaaa gacgctttga aagctgctat cgaaatcgcg gaagctaaag gaataaaggt
    24961 tgacggcaaa ccagcaactg tccatgacat tcaaaatttg acgaaagaac atctttattt
    25021 tatcgccgac ttgcttggtt tgtcggaatt gtatttagac aaataacccc acacggaagg
    25081 agtggctagg gtggttgtat tacaagtaat agtctgcgta acattcattt ggtcgtgcat
    25141 accggtttgt atatatatca tgaagaagct tcgccctgag tcttacaaat tatacgcaat
    25201 ttacagcata acagtagctc ttgcatttac attgtttgca atttctaggc ttccgtttta
    25261 atcgtgggaa tgaattttta atgaactaaa ttatctataa agcaaaaaaa ggagaaacaa
    25321 cattgaaaaa caaactagca aaaacaacag caatcatcgg actagcacta ggtagcggcg
    25381 ttatcggcta cgcagcaagc aatgcgtttc aagatttgga cacgatcaaa gcaaatttca
    25441 acacagtcct acaatacggt caaacgaaat cgcaacgtgt gtcagaactt gaatcacagc
    25501 tatccaacaa cactcgcaca caggagcagc tgaaagccga aattgagcaa atcaaatcgg
    25561 acaagcagaa ggaaattgaa gccaagcaat gagaagtcga acaaaagcaa caggagatcg
    25621 ctacaaagca acaggaagcc gatagcttgc gccaacaact atccacagtg caaaacgaca
    25681 aggagcagtt agaacagcgc gtgagcgagt tacggcagta tacggatcaa aaggtagggg
    25741 agttggggaa atgaaagagt tgactattta tgacccaaat atttcatggg ctaaacacac
    25801 aattaaagtt tcttttatga tttggggtta caaaggatat gttacttaca aagtgggcgg
    25861 taacactaag ggtttatcat taatagctat tgattcagat gatctatatg atgctaattt
    25921 cgaggataac ccagttaatt tccgtgacct tgatgaagat tggttctcta tggaacttac
    25981 taacgataaa ggcgacagta cacttgtaga agatgaattc gacagactag gtgattatat
    26041 tgtcggtgtt gaaattattg cgcacgaacc agagtaggag agtgaataca aaatgattaa
    26101 aagaatcact atatcagttg aagaagatag tggatcaaca atgcagcata ccgacaatcg
    26161 aaaaactttt ttaaccaata gaaaggagaa ttttgatgca gattaagaag gcgacggata
    26221 tcgaacaaac caagggaaca tatttgatct acggtgctcc aggaaaaggt aaaacttcaa
    26281 cggctaaata ttttccaggg aaaacactga tccttgatat tgatcggact tcaaaagtcc
    26341 ttagaggtga gaaaaatgtc gatatcgtat atatcgacaa cgaagatacc tggaacgact
    26401 ggggcaacac gttggctgat ttaactacca attatgtagg tgtttatgac aacgtggttg
    26461 tcgataatgt cagcgaattg gaacggtgta ttctgtccag tttaggcgca gaaggaaaaa
    26521 acaacggcgt tccttcgcaa ggcgattatc aatatatgca atttagaatg gtgaacagcc
    26581 tacgctatat gaaaaattta gacagcaacc ttatctggac cgcatgggaa gaaatcgatt
    26641 tgtggacgga ttcggatgga tcttcttaca acatcgcttt gccgcagatc aatcgaaaga
    26701 ttcgaaacaa tattttggga ttatgtgacg ttgttggtcg attgatggta aaggaagacg
    26761 atgaacgcgg atttatttta caagccacag attcaaccta tgccaaaaat caattggata
    26821 atagatctgg ctgtaagcaa agtgagctga ttttagatgg aattgtacga ctaccagaat
    26881 gatttagtcg atcgtgcaag acaagcctat gttgatggtt acaaggcacc atgtatcgta
    26941 tcaccgtgcg gttctggaaa atcagtcatg gttgcagaga tcgctaaacg aaccacacga
    27001 aaaggcaaca gagtgttgtt cttggtccat cgtcgtgaat tgattgatca gatcaaagat
    27061 acgtttcaaa agatgggcgt agataatcag cttgtaaatt tcggcatggt tcaaacagtc
    27121 gtacgacatc ttaaaaccat caagaaacca caactgatca tcactgacga aaaccatcac
    27181 gggttagcag cgtcctacag aaaaatctat gagtattatt cggacgttcc gcgattaggt
    27241 tttaccgcga cacccatccg gttaaacggt agtgggttgg gggatgtgaa cgacttgcta
    27301 attgaaggag tatcggctaa atggttgatt gagaatcata gattagctcc ttatgaatat
    27361 tatgcaccta aattgatcga tacagccgaa ttaaagaaag cttcgactgg agattttaca
    27421 aagaaatcaa tggataaggc agtgaagaat actatttatg gcgacgtgtt gaaacattat
    27481 aaaacgttag cagaaggtga acaggcaata gcatactgtc acagtatcga tgcaagtaaa
    27541 cacactgctg aaatattcaa cgatgctggt tataaagcaa cacatatcga tgcaaaaact
    27601 ccaaaagatg aacgtgcaga tattatcgaa gcgttcagaa atcatgaaat taaaatccta
    27661 tgcaatgttg atttgatcgg agaagggttt gatgttcctg attgttcgac tgtaattatg
    27721 ttgagaccta ctcaatcgtt atcattgtac attcagcaat cgatgagggg gatgcgttac
    27781 cgaccagata aaacgtcaat catcattgac catgtgggta atgtgaaccg ttttggattg
    27841 cctgatatgg atcgtgaatg gagtttagat acaaaaagaa aacaaaaact gatagtgatt
    27901 tatctatcgt tcaatgcgcg ttttgttttg gtgcttacga acggccaaaa ggcgataata
    27961 tttgtccgta ttgcggagag ttgcagccga ttgaagaacg aaagtcagag ttagaaatcg
    28021 atgaatcggc tgaattaatg aaagtcggag aaacgaaaat tactttgaat tttgagaaca
    28081 ataaatatta caacatgacg gaagatgaag cagaaagtat agaggactta tatgccatag
    28141 caagagctaa aggatttaaa cctggttggg cttatatggc tgctaagagg aaggggtggt
    28201 tgtaggaatg ggcggtattt ttggtaaaaa agcagaagat gttcaaggtt caataataaa
    28261 tggcgtaaca ataataggtg atactggaaa aagagatagt tcaggcaatc aaattgtaat
    28321 cgctagaaat aaaaataacg aagtttttga atcttctcta tctaatataa aaagaggtgt
    28381 tgttaacggg acaggcgaag agagtggcta cgttaattta aaaaaagcta atgaaaaata
    28441 ccatgttgaa ggaacttacg ttaaggcttt gtctgtcggt ggaacaaaag gcgttaattg
    28501 ggataaacga agaaataaat ggaaagcacg attatatttc aaaggagaat atgtcttaca
    28561 aaaggatttc aacaccaaag aagaagcaat ccaagcacga cttgcagcag aagaaaaata
    28621 tttcaaacct atcttagaaa aatatgaaaa taaaaaggag aatgattaac atgacaggat
    28681 ttaacttaga cttttcaaaa gcgcaacaag ggaacgaaat caaagacggt acgtacgaag
    28741 tggtcgtaaa caaagctgta gaaaatgcaa ctaaatcagg cgcagaattt atcgatatcg
    28801 atttgatcgt tcgtaacgat gtggatcaac cgttccaaaa taaacacatt ttcgcaaaaa
    28861 tctggaaagc caaagctacc ggcaaatata acgaaggtat gatcatggca attgcacaag
    28921 cgctacagtt ggaagacgga aaatcatata acgggtttga tgaattgctg gcggactttg
    28981 ttttgaaaac cgcctcagta cgcgtgaaaa ctgaagagtc aaatggatat aaaaacgtca
    29041 atgtgaagtc ttgggataaa acgaacacac ggggcgtaat gaatcaccaa ttcaaaaatg
    29101 gtgatgaacc atccttcgga ccggaacgtt caagagcgac aacggttaga aatgacaatc
    29161 ttccatttta attgaggtgt aatcaatgta tgaaaaaatt ccaaaagagc ttaaaaatct
    29221 caaacaatgg tgtgtttata agctagtttg ggatgaaaag cggcagaagc acacgaagat
    29281 cccttataac gccaacaacg ggcataaagc caaatccaat gacgagagta cctggtctga
    29341 ttttcagacc gctctcgctg ctattgatca ctacggaatg agtggactgg ggttcttttt
    29401 caaaccacct tatttcggca ttgatatcga taatgctgaa ggtgaagttg agcgatttaa
    29461 atcaggtgat atagaagaaa atattatcta tgaatttatc gaaagcatga gatcctatgc
    29521 tgagtattct caatcaggta ctggcatcca cattattgct cgcggagaat tgcccggcgg
    29581 aagacgcagg aaaggtgacg tagaaatgta ccagaacggc cgattcttcg ttatgaatgg
    29641 aaatgccgca tcaaaatatt tagaaataac tgagccgaat ccaaaggata tcaaacgcct
    29701 atatgatcgc tacgttggag atgaaaaaat catccaattc aaagaagaaa atccgctgat
    29761 gaataccgtt gatttaccgg ttgaagaaat tatcaaacgt gctgaatcgt cttcccaagg
    29821 ggcaagattc aaagtcttta tgaacggcgg ttgggaagct ttttattcct cgcaatccga
    29881 agcggatatg gcgttcgcca acgacttggc tttttggacc ggtagagact ttgaaaaaat
    29941 ggatgagatt ttcagagcgt ctgcaatgat ccgtagcaaa tatgaccaaa aaaggggatc
    30001 ggcaacttac ggagaatctc tactaaataa ggcaattgct gatacaaatt ccgtctacaa
    30061 ccccaaacgg aaatctgatt ttaagatttt tatcaaagac caggaacagc caaaagagga
    30121 aaaatactat tcgtacgacg acaccggcaa cgcggatcgg tttactgata tctacggaac
    30181 gttagtcaaa tattcgtaca ttgataaatc ttggtattac tacgatggga aagtttggct
    30241 acaggacaat accggcgaag ttcggaaaat gatcgatacg acagtagaca tcatgggaaa
    30301 agaaccgtta acgatccctg aaggcgcaga cgatgaaacc aaagaagctt tgatgaaagc
    30361 gaaagaaaaa cacgtcaagc gttctcgcag caatgccgga aaaaacgcta tgatggacga
    30421 attgaaacat cgactatccg tattgccgga agagttcgac aaagataaaa cgctgttcaa
    30481 cacacaaagc ggctatctgt ctttgcatga cggtcttcta cacgagcatg agatagacaa
    30541 aatgtttaca cgtgtttcaa atgtcgagta cacagaatcg gtcgattgtc ctatgtggga
    30601 agaatttatt aatcagattt ttgacaatga tcaagaatta atcagatata tccagaaatg
    30661 tgtcggatat tccctgaccg gatctaccag agagcaatgt atgttcatcc tctacggtca
    30721 tgggtcaaat ggaaagtcgg tatttttgga aattatttct gaattgatgg gaaactatgc
    30781 gatgactatg caggcacaaa cgatcatggt caagcaaagc caatcatcag caaacagtga
    30841 tattgcacga ttgaaaggtg cgcggttggt cacttcgtca gaaccgaatg aaggcgtccg
    30901 actggacgaa ggtctggtca aacaattgac tggtggtgat aaagtcacgg ctcggcacct
    30961 ttatggaaaa gaattcgagt tcgaacctga attcaagttg tggctggcaa cgaaccacaa
    31021 gcccatcatc cgtgggaccg atgacggcat ctggcgacgg ttaaatttaa ttccattcac
    31081 tgtccaaatc ccggatcaca aaaaggataa aaatctgaaa tttaaattgc agacggagtt
    31141 gcaagggatc cttaaatggg caatcgatgg ttgcctgttg tggcagagag agggcttgga
    31201 gaaaccaaag tcggttgttg cagcgagtca ggattatcga aacgaaatgg atcaaatagg
    31261 tacatttatc gaaacgtgtt gcgaaacagg tccaggacta aaaatatcag gcggagaatt
    31321 atacaaagtt tatcgtgaat gggcatcaga taatggcgag catactttca ctaacacgaa
    31381 gtttggcaga gagatttcaa agaaatatag caaagaaaaa gctggtggat ttatggttta
    31441 taaaggaatc acattaaaac caagaaaata cgataacgtt agagaacttt ttaaataaat
    31501 ctggagggtt ctggagggtt gagaacgaaa ccctccaacc cttagaaacg ttgatatatc
    31561 agtatttata ttatattttt ctttctttct ggagggtttt aaaaaaataa taaagtatta
    31621 aagtaaaaaa taaaaagtaa attatataaa aaagaatagg tttttgggtc tgaccctcca
    31681 aactctccac caaaatcttc ctaaatgctt tgataccaac gattaaacag tctggagggt
    31741 tacattgaaa tcagaacatc aaatacaaga cgagatcaga attgccttat caaaacatgg
    31801 ttgcacgatt tttcgatcca atgcgggaca agtgacaacg atcgaagggc ggcaatttta
    31861 cgggatgcca aaagggttcc cagatctatg tggacatcgt aattctgatg ggaaagcaat
    31921 ttatatagaa gtaaaaaatg aacgcggtaa gttgcgtgaa gatcaaaaaa ggtttgctga
    31981 atttttaaac ggtcaacctg tactatacgg tgttgcacga tcagtggaag aagcattgaa
    32041 aattgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat tcgaagcctt
    32101 cagcgtgaga ttgacgatct ggaacggaca aaggcagtga tggtcaatga aacggcgagg
    32161 aaggcaatcg atttgcacat agagaattta agaagggaaa tccgtagatt ggaggaatga
    32221 gcgtggataa ggaagcggct tggcgaaaat taatgttgct gattcaagat gagaactggc
    32281 aagaagatga agcagtggtt gctgaagttc agcgtctagg aaagattgct aacggacgta
    32341 tacgaaaaaa gccagacaaa agaaaacagc gcaaagggaa aatcgtcgtt gttttacacg
    32401 aaggcaaaat tttgatgcaa ggaacagcta gtgagctgtc tgcagaaact ggatatacgc
    32461 gtgggactat tcgaacgtac gcttggcgaa atcacaccga tcgaaaaggg cacgaatata
    32521 agtatttgga ggaagaaaaa tgaacgaaaa caaattaatc aaattgggtg tagcaggagc
    32581 agtaatcgta ggtattggag ttatcggagg atttaagttc ttcgaaaaaa tcgataatgg
    32641 atatgtgggt gtgcgctatt caatgaacgg cggtatcaaa gatgaagcac tgacgcaagg
    32701 tgtgaaattt gtagggattg acaaagtgat ccaatatcca attcgcttgc aaactatcca
    32761 atcaaaaaat atttcagtat ctacaagcga cggcaaaaag acaacgattg atatcaaata
    32821 tgactacaaa gttgattcaa ctaaagcagc aaaaatgtac aaggaatttg ggaatatcac
    32881 ttcggaagat atcgaaagtg gatggttaaa atctaagctt caaaaggtcg ctcgtgaagt
    32941 ttatgcgaaa tatagtctgc ttgatgtcct ttcaggagat tcctctaaag ttgaagctga
    33001 ggtattaacg aactttgcta aatcagttga atctaaaggg tttgaagtcg aagacgtaac
    33061 acttggtgtt ccagatgtcg ataaagaaac acaaaaatca atcgatgcga tcattcgagc
    33121 tggtcaagaa aatgaaaaag cgaagctaga cgcagaaact gcaaaaactc aagctgatag
    33181 tgaagcttac aagaaaacaa aagctgcaga agcagaggca gaatctaatc gcaaagtcgc
    33241 cgaatcagta acagacaatt tgattcgtta tgaagaagct caagctcgca aaaagcatgg
    33301 atgggtaaca gtaaatggag cagatactgt agttacggat gaagcaggca aataatatgg
    33361 gattctttat ggctaaaatt ctcttgttct taggtttagt aggagcagca tatctcgtgt
    33421 atgcgctcct ttcccaaact gatgacaaag aagatgacaa caacgatgaa atgaaatttt
    33481 aggaggagaa ataatggacg aactaatcac aaaagtagag cagtgggcta aagacaaggg
    33541 actggatcaa gcagatccaa aagcacagtt tttgaaagta gctgaggaat tcggggaaat
    33601 agcttcggcg atggcaagaa gtaatgatga actatttaaa gatagcgtag gagacgttat
    33661 cgtcacgctg attatccttt ccatgcaaaa agggacaaac gtacaagagt gtttagaaat
    33721 ggcatacaac gaaatcaaag gacgcacagg gaaaatggta gatggtgtat tcgtgaagtc
    33781 gagtgatttg gaggatgtga aatgaaatac gaaataccac taagtgaagc gggcattcaa
    33841 gcaattatca atggtcggga ggttaacata gaacttcctg atggtactga attagtcatc
    33901 agacaaagtt atttgaaaga tatggcagct ccagtattaa ttgatcgttt taacgtgact
    33961 gattctgtgg tagagaacca cttaaaagaa tttcgatcaa gtatagacaa cactttcaga
    34021 ttagggagtt gattgacaat gaacaccaga caccgcagaa taacaaaact aagaaaacag
    34081 gaactaaatg tactaaagac aaagtttgaa aaagaatatg gaatttcagc agaagaaaca
    34141 tataaagtgg caagtcagtg tgttgctgat gcgagcggcg ctattcgtaa gtttggaatt
    34201 tcgatattaa atgatgatcg taaatgggag gaaaaagaat gaaactaaaa gacggatttt
    34261 actccagcag tcatggtatc ggcggtttaa tgctagatat gccgacaaag aaccctaaaa
    34321 cacgtaagaa accaaaagtc aaagtcggtg acatggttcg ctgtgaagca gaagagttca
    34381 tttatccgtt tcgtggatat gtagaacacg tctataatca ctcagcgatc attcgtattg
    34441 aaaacacaat ggaatgcgat aagtggttag cgaaaagcaa agagaattta gctgtagcga
    34501 gattggtgga tatggaggtt ataaacaatg aagtttaaaa tctttgaaga ggacactcgt
    34561 tataaattag aaaaagaatt aaacgatttt gcgaaaaaca atgagattca gcatatatct
    34621 ttagcaactt ctaagagagg ttatgcaaat tactatgcag ctgttgtgag ctatgtaagt
    34681 cgagaagtgt aactcggcaa ataaaaaaag ccggatcgct ccgactgatt caataaatcc
    34741 aacacattta ttatatcaca taaaggagcg gtttgacttg atgcaattgt tacgagaggt
    34801 agatttcaaa cagacaagat gtaatgcgag agatgtgctg aagaactttc ggcgtttgga
    34861 gcggatggca ggtcgctctt tgatagatat taagtcgccg attattacgg atatgccgaa
    34921 ggcgccgaag cacggcaata aggcagaaga cgcaatcatt cagatgatgg atatagaagc
    34981 ggagagagac gcgattctag cagccttgat ggctcttagt ctgattagtc gtcagatact
    35041 ctactatagc ttttgtgtgc cagatagctt ctcaaactac agaattagcc gtgaagtggg
    35101 ttattcagaa agaagtatac aacggatgaa gtcggaagct ctaatagagt ttgcagaagc
    35161 atataaacac ggaagaataa ttgcttataa atagggggac catataatgt ggaaagatta
    35221 tgtatcgttg aaagagttga aaaaagatct tgttttcaaa agaattgtag aatggtcaga
    35281 gagtgaatta attttagaag atggaaccaa aatggaagtt gtatgtagcg aatcagattg
    35341 ctgcgcatgg gccgaaggtg aattcaaaaa tgtaaagctt gatgcagtga ttacagatat
    35401 aaagattttt gataaaggta accgtctcta taatggtgac ggacatacct cttatgctga
    35461 agtcgttgtt tatcataatc gtaatgagat cgctaaagca gaatgcacag cgaatgatgg
    35521 gaatggcggc tactattata gtgtttgtgc tttaaaggtc aaagacaaac tttgtatagt
    35581 gactgacgca taaaaaaatg gcggtttttt ggcggaatga tggcggtttt tagccattta
    35641 tcagtgatat tatggtagtg tcgaaagatt agtgataggt ctgagacaaa ataataataa
    35701 aaggaacatc gttttattat tgtttcacaa ttaagcttcg atagacagca acggaaatat
    35761 taagaataag gatgtgaatt ttaactcctt ctaaattgtt cttattatct atcatccgtt
    35821 gctgtctatt gttacataca gatcgcttag gcggtctttt tattttggcc gaaaacctac
    35881 attttcgatg gccgattatt ggaggaggaa tagcatgttc aaactatctg aaatcattaa
    35941 gaagtccgac gttgagaaat tagagatgtt aaaaaagaag ttgaagaaat agcagtctct
    36001 tcgtgaggct gttttatttt gctcacaaaa atagaccact atcgggtaat agtggtcagg
    36061 aattaaatga aaaagatgtt agggttgtta gctaagtata acatcataac gcttacaaaa
    36121 caatgcaaga aggataaatt attatgagaa cttactggta tgtgtcatta aacaataaat
    36181 atccgaagcc aatgaaagga cagcataggc gtgtagtcat gtctgttcaa atgaaggcga
    36241 agtattcgat tgtagaaatg ctaagagaag ctacaccaaa cgaagtggat aaatgcaagc
    36301 tgatatattg cggtcatggc tattggaaag acgagtatat ccaacaaaac attgagaggt
    36361 ggatagatag atgagttacc tcgaacattt gaaacgttgc tacatgcatt ctaagaataa
    36421 acttcctgac agctacactg tagatgatgt tgctatacat gtattgaaga ctgagagcca
    36481 tagcagtcct gatggtagca gtaaggagca gacgctcgcg tggttcaagt tctttaagtg
    36541 gattaaggag gaagagtaat gcacatgcac ataggagaaa agatatataa attgagacgg
    36601 cttaataacg aaacaatgaa cgacttagtt aaacaaactg gagtgtcaaa atcatggtta
    36661 tctgatattg agtctggaaa aaagcaacgc gttgatttta ataagatatg tgcaatagcc
    36721 aaacattata aagttagctt agaatacttt cgggatgatt atttggatag tgatgaacac
    36781 gaggatatta ttcctttgac aggcgaaaca agagttaaat cagtgaaatc gcttgaacaa
    36841 agcctaaagt atctgaataa caaacaagaa cctaaccttc tagagatcaa actaaaagat
    36901 actgactcag taccagaggt ttggtacaag ggtgagaggt tggatgaatt gcctaaagga
    36961 ttagtagatg tctcgtatca ttggaagact gatgatttta ctaatgatga tagaggagcg
    37021 aacgacatca cgattcaata cttttctagc tttaatgata agtatccaga taagaaaaca
    37081 atcggacaca agagagatat gtaaatgaaa gaagttagac ctagagacga aatagacaaa
    37141 ctatacaaga ccaaacgatg gcgagaccta aggcaagtag taatagctag ggacttcggc
    37201 atgtgccaag agtgcaagcg tcgagggcgg aacacaaggg gcacgatcat ccatcacata
    37261 gtcgaggcga gggaagacct gtcactgttc tggtccgtag ataaccttga atgtatctgt
    37321 gtagcttgtc acaacagaga gcatccagag agatcaggcg ggaagaagaa accaaaacct
    37381 aaatcacata tcgttaaaat gtattcaact cctgaaagat aagtttgcag cgaaatgaag
    37441 gtagcccccc tactctaaaa gattaaagag taaggcttga gaagaacggt gctgtccttc
    37501 cttcgtaaaa agaccgcttt tcaagttttt tggagaaaaa ggaaaaagcc gaccaattta
    37561 agccggcttt ggacgaagct atttcttagt ccatttgttt cctttttgag aagtaggggg
    37621 taatcggtcg cctggatcaa tttttacttc ttgtccgcct tggacatttc caccacgagg
    37681 acccacttct ttgtaggttc cttttggttt gttgtcttcg ccgggtttat agagttctcc
    37741 cataggaatc cctccttaac aaatttcggc acagcactgc cgataaccta attataagga
    37801 ttgtgataac gatttcaatc tatcttttga aagaaggtga tattatgccg caaccagcga
    37861 agagtgcaaa attacaatta ttaaacggaa acccaaataa gaagaatacc gaagaactcc
    37921 gcaagcgagc ggccgcagaa gacaaattaa aaatggctac tgacaaaatc aaaccgccgt
    37981 tatggctaga ttcgctagga aaggatacct ttgagtttat cgccgatgaa ttgctgtctg
    38041 tggatttaat cagtaatccg gacgtccata caatggctct atactccaat tggtattcgc
    38101 aatacgtttc tttagaaaaa cagcttcgaa aactacaacg agagtacaag ttgaactatg
    38161 cgcttgcgaa aaaggaggca gaggcgagag gtgagccgtt taatgaacct aatgaattaa
    38221 ttggtaatcc gctctctcgg cagatggata cagcgtctcg gaatctccgt tcttttggcg
    38281 ctgatttagg actatcacca gcagccagag ctaagttagc tattaagatg gctgatgatg
    38341 gtggtgatga cgatgacgac ttctaatatt ttggatatgt cttacacaga acgtgtggac
    38401 tattggcaaa gctatcttga ggagcaagct tcttggggtg gctttttaaa atgtccatat
    38461 ccggaattgt taactacttg gtatgcggaa cgattaatcg atggaagcat accagccagc
    38521 aaagaaaata ttcaagctgc taaacggcat atgcgtgatt tgcagcgcca aggaacagat
    38581 gattttcctt ggatctttga cgaagaaaaa ggtcaccggc ctattagata tatcgaaaaa
    38641 aaatgtaaac caactgaagg cgactttggt tcgtttgttt tgcaaccttg gcagcatttc
    38701 ataattggat ccatgtacgg atgggtacat cgtgatacag gagagcgtcg cttccgcgag
    38761 gctcttattt ttgttggacg taaaaacggt aagttttgcc gtttaaaaat cgggcaaaat
    38821 cggtgaaaac ctttattttt ggctttcttt tagtaataat tcgatagctt tatcaagcaa
    38881 tttagacata ggaaccatag tttcttccga catttttttt aatccttcgt ataaatcttt
    38941 atcaattgcg ttagaaattc taattctgtt ttttagtccg tatttgtcca tgtgtgacac
    39001 tcctttttat tttactataa catccgattc caccgcttgc aactaccatc aattgatggt
    39061 agtgtataaa agaggtgagt agattgaaaa aatacattat ttacaaacac ttaaataaaa
    39121 taaatggcaa aatttatatc ggtgtcacaa atgatattgg cagaaggtgg aggagcggtg
    39181 ggattgaata taaaccacca aagaacgaaa cccaacacca cagaagtttt tggaatgcaa
    39241 tacaaaaata cgggtgggaa aattttgatc atttgattat tgaagaagac ttaacgatga
    39301 aagaagcttt tgagaaagaa aagttttata ttgaacttta tgactcaact aataagaaaa
    39361 aaggttataa catcgcaaaa ggcggaaatg gcggaataat atataaagtt caccccaaag
    39421 gaatgctagg gaaaaaacaa tctaaagaat tttcttcaaa tcacagtaaa tgggcaaaga
    39481 accataagaa taattgtatg acaaacggtg atgttgtatg gggtgttact cacgaacacc
    39541 caaaaggcat gttgggaaag caccacagca aagaatcaat tatgaaaaag aaagcatata
    39601 gtggtgaaaa tgctgtaacc tcaaaaccaa ttgtcgcaat tgaacgggat ggaactaaaa
    39661 gagaatttca cagcgctaag ctttgcatgg cttactattc gattagtaca tgtgtttttt
    39721 accgactttt aaaagatggc gccccttatg tgataaatcc taaagcgaac tacagaaata
    39781 aagaaaaaat attagctatc gaaggaatta tgttcaaaca taaagaagat accgaggtaa
    39841 gttagtagat tgcgaaaggt tactaactac cgtaacgcgt aggagttgaa taaatataat
    39901 gctcccaaga gtgtccgaca gcagataatt tgctgaaaat gtacgctaaa ctgggccaga
    39961 actgactgac cgatgaaaat gagggtgacc tccagagtgc gagataaaaa gctcgcagat
    40021 aataacaatc gaaaacatca ctaatttcag gcctttccac atacatggtc gcttatgatg
    40081 atgaacaagg cgccaacgtt tacgtattgg caaatgctcg tgatcaagca agcttgttgt
    40141 ttgataaggc cgcagaaatg gtcaaacaat cgccggcgct ctttaagaaa tttggtaagc
    40201 ctaaacgatc aagtattaat tatgctcccg ccttttctaa aatggaacca cgcgcctcag
    40261 atagccggaa attggatggg ctaaacactc actttggtat ttttgacgag atccacgagt
    40321 ttacgaatta caatctgatc aacgttatca agaaatcaag aggaaccaga aaacagcctc
    40381 tgatagttta tatcacaact gctggatatg tattagatgg tccgttgatg tcttattttg
    40441 agcaaggtgt ggactgtttg gaacatttgg aagatgacat cgatgaacgg actttctatt
    40501 atctggcaaa acttgacagt gcggaagagg ctgatgaccc aagattatgg atcaaagcca
    40561 atccgaatat ttgtctaatg aattttgttg gcatgctaga tgactatgtt aaggataaaa
    40621 aagatccaaa agaatatgct gactggatta ccaagcaatt taacttgttt tccgatatcg
    40681 atgagctgtc atttgtcgat atgcctacca ttaaacgaaa caataaaacc atcgatattg
    40741 aaacgctcaa aggtaagaag tgtgtcggtg gttttgactt gtccgaaacg gaagacttta
    40801 ccgcagccgt tttagaattt ccgcttgaaa caggcgaggt attcattttg caacacacat
    40861 ggatcccaca agctagattt gatcgagata acaatcaaga gcgtatcaaa gcgtgggaga
    40921 aggtgggaga tctaacgatt attcctggtg attacgtcaa ttatgaatac gtcttaaatt
    40981 ggtttgtaga aaattcgaaa atctatgaca ttgtaaaaat caattatgac aaggccaagg
    41041 cgctacgatt aaataaagaa ctagaaaatg caggatttga aaccaacgag attcggcaag
    41101 ggtttctatc attaggtggg ccaatgcaaa acttcaagga aatgctattg gacggtaagg
    41161 tgattttcaa caattccaag ctttaccgat ggtatctatc caacgtcaag ctggtgatgg
    41221 atcgcaactc aaactggatg ccgtctaagc agtccaagag tagaaaaata gatggttttg
    41281 cagcaagttt gaacagccac gccgaagtgt tgaatatgtt ggttaatcct gtcggaaccg
    41341 ggaaagtaac ctattactcg atttccgatt taatgaatat gtaagaaagg tgtggaggaa
    41401 tgagtatttt agatcgtttg cgttcttttg gccgagcgaa gccgaaagcg agcaaacaag
    41461 agtatttttt gaatgacccg ggattgatac cgtatttagt cggaaaagat gaaatatcag
    41521 aagggatttt ttccgtaatt agccgtgtat cgaacgtttt tgcgtctctc cctctcaaaa
    41581 tgatagatgt ggagtttggc caaccggacg actgtcctgc atacaacttg ttgagcgaag
    41641 gccctcgata ttttacaaag tttgattttt tccgggacgt ggaagttttg agaaactacc
    41701 aagggaatgc gtatgtgcag attttccgaa atatcaatgg agaagtagca gatatggcgt
    41761 tagtaaaacc tggtgcttgc catccagtga ttgatatgga tagcggggag ctttactacc
    41821 aagtaactgc gactgacaaa ggcagttaca agcaagttat ctatgtacat tacatggaaa
    41881 tgcttcactt taaacaaccg aggtttggcg gcttggaagg tacagacccc acaaaagtat
    41941 taacgaatac cctcggatat gatcgagaag tccgaaaaat ctctttaagt cagcttaaag
    42001 gaagtaatga agggctaaaa gttaagtttg ctagcaatat ggatgaagaa gctaaaaaag
    42061 ctacagttaa aaacattgct gatttttatc gacaaaacgg tggactactt gtggaagaaa
    42121 acggtgtaga aatcgaacgt ttacaacgag agctggtaga cagcaagctt ttagatactg
    42181 ataaagtatc tcgctccaga atcgctatgg tctacaatgt gccggagcat ttcatcggga
    42241 ataaccagtc gagttactcc tctcaggaac agctcaatat ggaatttttg acgtacaatc
    42301 tagtaccgac cgttaatcaa tatgaagcgg aactaaataa gaaaacacta tcgagaaccg
    42361 aaaaagcaaa aggttatcga tataagttta atgtcgcaag tttgctaaga gctgacactc
    42421 aggcaagggg acaattctat caaatcatgc gacgaggcgg agcatactca gcgaatgatg
    42481 tccgaagatt tgaggacttg caaccgataa ataaaaccgg tatggatgat taccatattt
    42541 ccggagacct atatccaatc gatatggatc caacattaag aaaaacaacc tcgtctaaaa
    42601 gcgtagccga aaacggttag gtttttttag tttgcaccga agggaggtgg aaaaatgaaa
    42661 aaagtgacgt taagcggcga tgtcgtggat aacgataccg cgtggcttta tgactggttt
    42721 gggatcaatt gtatctcacc agggaaaatt tctgccgctc ttacagaagc agcgggggat
    42781 gaagtagaac ttgatatctc atcgaacggt ggggatgtcc tagcggcaag cgaaatatat
    42841 accgctatcc gtgcctatcc gggaaaggta tccggaaatg ttgtgagtat tgcagcaagc
    42901 gctgcaagtg taatcgcttg cgcttgcgaa ccgcttagaa tctcacctac ggcacacatc
    42961 atgattcata acgcatgggt gaccactagt ggcaacgctg aggaattaaa agccaatgca
    43021 gaaatgttaa gcagtgtgga tgagtctatt gttaatgctt acgagatcaa aacaggactc
    43081 gatcggaaaa aacttgctga tttaatggcg aaagatactt ggttaaatgc tcaaacagca
    43141 gtagcagaag ggtttgcaga tgaaattatg tttgcagaag caccagtaac ggtactcaat
    43201 gcctctcaac cggttattcc aaaaaacgca gtaactaagt tgaaaaattt aatactcaaa
    43261 gcggaagcac cgcaagaaga aacactctta cagaaaaaac taaaagcctt aaatggaggg
    43321 aaaaacgaat gaatttagaa caattaaaaa atgcgtgggt cgaggcggga agtaaagtct
    43381 ctgacttaaa tgcacaactc aatgcagcat tggttgacga tgaaaaaaca gaagaagatg
    43441 tagtaagttt gcaagcacaa gtaaaagcag cacgggctaa acgggacgga ttgaaagagc
    43501 aagtggcaaa tatggaagcc gaacaagtct taaacgtcaa aaaagaacca ttagataaaa
    43561 aagatgaaaa cttgaaaaac aagtttatca aagactttaa agcgatggtc aatggtgatc
    43621 ctgctattat ggctactttg acatctaata cggatgaatc tggtaatgct atcggattga
    43681 ctattcctgt agatgtgcaa acgactattc atactttggt tcgtcggttt gactctttgc
    43741 aagaatacgt aaacgttgaa aaagtgacca ctaccagcgg ttctcgcgtt tatgaaaaat
    43801 ggtctgatat tacaccgttg actgctttgg atactgaaga cggtgaaatc ccagcaaatg
    43861 atgatcctgc actttacttg atcaaatact tgatcaaacg ctatgcaggt atttccacag
    43921 taactaacag cttgctaaaa gatactgccg aaaacatttt ggcatggttg tctaaatgga
    43981 tcgcgaaaaa agtagttgtt actcgcaata caaaaatctt ggcagctatt gatggaatca
    44041 aagcggcaca aaagaaagat gttacagatg ttgatggaat taaagatatc gtaaacgtcc
    44101 aacttgatcc agctatcgaa gctacatcta tgtttattac aaaccaagat ggcttcaatg
    44161 ttttagataa agtgaaacgt gctgatggat cttacttgtt acaaaaagac gtagcttctg
    44221 caactggata taccttctta ggtaaaccaa tcaagaaaat tgcttctcgt ttcttgccaa
    44281 acaaaggtac gcaagctact cctaaatatc cactgtacat tggtgatctg aaagaagccg
    44341 ttacattgta tgatcgcgaa aacatgagct tgctgacaac gaatattggt ggtggagctt
    44401 ttgaaacaga caccactaaa gtacgcgtca tcgatcgctt cgatgtgcaa ctagttgatg
    44461 atgaagcggt tgttttggct acttttacaa ctattgcgaa cgagacaccg gcgaaagttt
    44521 aaggagctga ttccttatga ttcttgatcc taaaatggat ttaggcgaaa tcaaaaacgc
    44581 actaaagatt gataccgatg atgacgatgt ggaagtaagc cgtgcggcac aagctgcaat
    44641 tgcatacatt agaggggcta tcgggaatga taagccctct ttttataagc aagaaaatga
    44701 aacggttgat ctgattaatt tagctatcct gcaattagca gatcattatt acaacgctcg
    44761 atcagcaacc gtgagtggga acttgcgaga gtacgattta ggttttacaa gcctaatctt
    44821 gcaactcaaa gcaagttatt tgctttttgt ggaggaggag tagcgtatgc cccttatcca
    44881 aacaggaaat ttaaatcaac gcatcaagtt tgtccgagat acgacggtta aggatgagga
    44941 cgggcaagtt gtcccgactt ctacaaccat tcttacttgc tgggcaagtg tgcagacaca
    45001 acgcctgaac gatattaaga cgtcgattgg tacggctttg gaaggaacac tgacgttcat
    45061 tatccgctac caacaaaaat cagagctgac caatgatatg aaagtgcgtt ggaatggaaa
    45121 aacgtttgaa atcattacga ttacgaaagg cgagtttgcg aaggacttca cgacaatcat
    45181 tgcaaaagag gtttcaaaat gagtgtagaa gtcgatgcaa ccgaagtgta caaagcgctt
    45241 agggaagtaa aagcaaacgt tcaacgagtg gaaagcccag cacttagaaa agctggggag
    45301 tacgctcaag aagagttacg acaaaacaca ccttactggg atggaacgaa gtcaaacggt
    45361 aaacgtggtt cgtatatgca agaacatgct aagaaccatg tggttacaag ctcgataaaa
    45421 aacggattgg tagaagtcgg ctatgacaaa gatgtttctt ggcggatgca ctttatcgag
    45481 ttcggaacaa tcaaacaacg tccaaaaggt ttcgtacaaa aaacacaaaa gcaaatcgaa
    45541 aaacaagtaa cacaaatcat tgctgacgaa gtaaaaagga ggctaggact ttgaaaacgg
    45601 cagtatcaca agtctattca attctgaata gcaatgaaaa aacaaagaac attgattttt
    45661 acaccaatag tgttccggaa tcagctcaaa cagtacctag ccttccagtt ggcagaatta
    45721 cagagatatc cggcaactat gaagattttg caagcaacaa tcctttgacc attcaattta
    45781 acgtacaggt agatgtatgg gtgtcaacca tgaaagaggt tgatgccttt tattttgccc
    45841 ttgatgaggt tatgaggggg aatggttggc aatgcgcata cacggaacaa acagatgacg
    45901 aggacttgga aggtgcaaag cggattatca aacgatatgt agcaaatatt tcactaaatt
    45961 aaaaggagag aaaatagatg gcaacagtag gattcgagag cgtcattttt ggcgtaaaaa
    46021 caggtgcagg cggcactcta aaagaattag tagcagataa gtcgaaaggc ggagcgatcg
    46081 aagctaaaat tactggatta ggcgcaactt ctaacacaac atacgcttca aacgtaccgt
    46141 tcttcattgc aagtaaaggg gtttcgtcgc caaaagttac gcttgacgtg gcagacttaa
    46201 tggataacgg catttacagc gaaatcattg gtgctaaaac cgtggatggt gcaaatgtaa
    46261 ttggttcaga aactgaagcg ccttacgtgt cggtagtcat ggttacagcg aacaaagaag
    46321 gaaaacgctt gttcatggga ttgacaaaag gaaaattcag tcatccagat atcgacatga
    46381 aaacagctga agacaaaggg gtagaattgc aaaccgattc catcgaaggg gaattcattt
    46441 ctgatgaacg tggctatgta tacttaacag ccgtagaatc agaaggaatg accttacaaa
    46501 aattcaagga cttggtaaat aacaaagagg gggagtagtt aaccctgcat ctacaccaac
    46561 gacagataca gggacaccaa aagaaccaga accaaaaatt gatacacaag gttagccatt
    46621 tttggctagc cttatttttt gtaaaaacaa ggaggaaaac aaatgattga attgcaattg
    46681 aaacttgacg gaaagaaaaa aacattcaaa caacaagata tttccgcacg tgcaatgcgt
    46741 gagtgtatca aattttacga gaaagcggaa aaagcagacc taactgattt agaagcgatt
    46801 gattcaatga ttgcaattac agcagatatt ttccaagatc cagcagttac atttgatgct
    46861 attttagacg gtttgactgc gagcgagtta gtaccggcat tagaaagtgt ttttgaacaa
    46921 ataaatgaac tgggaaacaa tgaaaaaaag cagacggcga gcaaaaagaa ataagttttt
    46981 ctgaagctag gaaagcaatg gatcaaatct acaaagattt aatcgaagca ggttggacga
    47041 tgagagatgt ggacgaagcc gactatcatt atttgttaca cctttttgga gaagtggaga
    47101 gtggcgaaga atatgtagat ggtgctgatt tcatcaaaca atttttatcg gctgaagact
    47161 tagtaaaact tgaggaagga ggtaaataat ggcaggaaaa ggacaaccgg caggaaatat
    47221 caagctaggg attagtttag atagcactag ttttggtaac acgctggacg aaatcaatgc
    47281 gaaagtcaaa caagctgagt cgaatatgcg tgccaatcta aaggcttatg attcagcagg
    47341 acgttcatac gaagcactta gtcaaaagac gaaagacttg tctacggtta tggaagggca
    47401 aaacgccaaa gtaagagaat taacaaagcg ccgtgatgaa gcgattagca agtatggcga
    47461 ggaatcgaaa caagttgcta accttaacac acagataaac aatgctaccg caaaatataa
    47521 tgcttacagt cgccagttga acgacacaaa aaaagaattg gtgtattcca aaacagccgt
    47581 caatgattta tctaatgaaa tcaaagaaaa tgaacgacaa atgaacgccg aagtaaaagc
    47641 gttgaaagcc gctggtgatg aatctggtgc ttttgaagca aaacaaaaag ggctagccaa
    47701 acaaacggaa ttatccgaga aagctatcga agaacagcgc aaagttgtga aactgatggc
    47761 tgatgagttt ggcgattcag caaatgaaac cgaagatgca aaaagggcat tagaaaagtt
    47821 agaacgacaa agccaaatat ctagcaggca attagaagca ctcaaaagct ccagcgatca
    47881 atcaggaaaa gaaatagaag attttggcga caagtccaca aggtcagcta ggaaactgga
    47941 cggactaaaa gacaaattaa gctcgctaaa aagcgcattt tcgtttggtg cagttgctgg
    48001 attagcgcat aacgctatta gcagtgtagt aagtggcgtg caaggtttgg ttggcgaagc
    48061 agtaaacgca tcggattcat tgatgaagtt ttccaaaacc atggagtttg ctaactttgg
    48121 gaagtcacag atagaaagct cgaaaaaaga aatgaaagac tacgccgata agacggttta
    48181 tggtttagaa gaaattctga acacaaccgc acaattggca tctaatggga ttcctaacta
    48241 tacagaacta accaaggcgg caggtaactt gaatgccgtt gcaggcggtt ctagtgatac
    48301 attcaaatcc gttgccatga tgctaacgca gacggcagga gctgggaaac taacaactga
    48361 aaactggaat caattagcag atgcgatacc gggtgcttca ggattgttac aagacgctat
    48421 gttgaaaaac ggagcttata caggaaactt ccgtgatgca atggcgcaag gtcaaatcac
    48481 ttccgacgag ttcaaccaag caattgtaca gttaggtatg aatgacggag cagttaaggc
    48541 agccacttct acagacacat tgagcggttc ttgggagcag atgaaatcca ctgtaataaa
    48601 tgggctacaa agtattatag aaaaaatagg cgttgaaaat atcactggtt tcatcaacag
    48661 agtaacaaaa gggattgaaa attctattcc taaaattact caatttatag gttggttgag
    48721 agatattgga acgtggatcg ttgaaaatag agagccacta acatggattg tcggaatcat
    48781 aggcggaatt acattagcag taaaagcatt gaacgtagca agtatgttgc tggcaattac
    48841 tggcggaaca ttggcagccc cttttgtggc gattggtgta gcattaggcg cactagcagg
    48901 tgctttggtg atagcttata caaaatctga aacatttaga aatatagtca atgcggcttt
    48961 tacagctgtg aaaaacgtag ttatgagcgt tatcaataat ttggtggaat actacaaaat
    49021 gttgtggagc gtgttgcagt ggctttggga aaaaataaaa gaatgggctt catggattgg
    49081 caataaattc attgaaatga agaacagcgt tgtgaatacg gttcagaacc tatggaatgg
    49141 cgtgaaaaac ttcttcagca atggcgttgg agatacgtgg aataaggtag tcggttgggt
    49201 aaaaaacatt ttcaacaaag caactgaatt gaagaacaac gtttctgatg taatcggtaa
    49261 cctgtggaat ggtatcaaag acacattccg tagaggtatc gatacggtat tcaattggtt
    49321 ttcagaacta ccaacgaaga tgaagaatgc cattattggc ggtaaaaacg ccattgttga
    49381 tgcgttcaaa agtattttca acgcagcact taaagcgata ggtaaaccag ttaacgcaat
    49441 catccatgga gcttcatggg tactagaaaa attgggcgca gaacctctac aagaatggga
    49501 tgtaccacaa tacgctacag gtacaccagc aggtgggcac ccaatcaatg gtccaatgat
    49561 ggttaatgat ggacgtggag cagaaacagt tatcacacca gatggtagag ccttcattcc
    49621 taaaggacgc aatgttgttc taaacgcacc aaaaggaacg catgtcttga cagcagaaga
    49681 gaccgcgcaa cttcaaggtt ctaaagctcc taagtatcgt tacaaaaaag gtactaactt
    49741 ctttggtaac atgtgggata gtgtgaaaaa ggttgctggt aatgtaggtc acacaattaa
    49801 aaacgtagta ggtgacgtgt gggactttat ttcagacccg ggagcgttag ctagaaaagt
    49861 acttgggggg ttagatgtat taggtggatt gacaaagtat ccattagaag taggtaaagg
    49921 catcctatct aaagcaacaa gtgcactgac tgaaaaaatc actgggttgt tctcatctgg
    49981 taacttagat acctccgtag gaacaaatgg cgtctataaa tatttggctg atgttgctaa
    50041 gtctgtgatg aagaaattcc caggatttgt ggcaactagt gggtatagac caggtgaccc
    50101 ttattcacat ggtaaacgta atgccattga tattgcatta ccaggcgtca caggaggctc
    50161 acctagatac acagaagcag caaactacgc ttttgacaaa ttcgcttcca aaattggtta
    50221 cgtaatcact aatgggaaag ttcgtgaccg ttcaggacaa tcaggtcaac cagcaactgg
    50281 tgcatgggag ccatggcccg atggagatca ctatgatcat gtgcatttaa acggtgtgaa
    50341 agacccacaa aacactcaaa tttcaggaga tagcgtggga ggcagtgggg tagaaagatg
    50401 gcgcaatgta gcaattagag cgttgaaaat gaccggtcaa tacagtactg caaacttaaa
    50461 tgcattacta aatcaaatgc gtacagagtc aaatggtaat cctaatgcag ttaacaattg
    50521 ggatattaac gccaaaaatg gaacaccatc aaaagggttg ctccaagtga ttgacccaac
    50581 attcagacag tatgcaatgc caggattcaa cagcaatatt tttgacccac tatctaacat
    50641 cttagcttca atcagatacg cactatcaag atatggctca ctaacaaatg cctatcgtgg
    50701 agttggttac gcaaacggtg gaattgtaaa ccaacatcaa attgcggaaa tcgcagaagg
    50761 aaacaagcca gaaattatta ttccgttaga taaggctaaa cgatcaagag cgatgcaatt
    50821 attgtctatc gcaatggaca aactaggagt ttctcctaaa acttatggaa atgcgacaac
    50881 tgtttccagt gattacgaaa tcctagaagc cattgaaaaa caggcggcaa caacgaatca
    50941 gctgttatcg ttattgctgg catttttcaa aggaaatagc cgaactgata gagatttagc
    51001 tttagatatt cagaagatct tggttaggag gatgtagatg cgaactgtat tactgagaaa
    51061 tagaataaac gaagaaattg atttgtccac agaagactat tttgctactg ggttgagtaa
    51121 tatggggttt gaagtaaaaa aagaacatgt gggacagtgg gggaatttca gagaaagcag
    51181 tgagagcgtt gaaatatctg aatttcagtc atctgtaatc atttctgtgc atgggtttcg
    51241 agaaaaagaa ctgtacaatt cacttgtgca gttcctatcg gaaggtccgt ttgaactgga
    51301 atttgctttt gacggcgaaa caatggtaag aagatgcagt ctaaaatctt taagtaagac
    51361 tgaaatcgat ccgaaaacat cgttactaac agacacttta gaactatatt tcacatcaaa
    51421 ctggtattcc gtaaaacgcg aaaaattaat tcagcgaccg aatgtagtga aaacacgtgg
    51481 taaggttttt ccgtataaaa gatcttatat ctacactcaa aacttgtggg agaaaaaagg
    51541 tgtattcaaa ttcaataata attctgtata cctaacgaat agcaaggaac ggatgtcccc
    51601 gctaaaaatt cgtgtgattg ggaagtgttc aaatccgtat tgggaagtaa tccaaaactc
    51661 acaaatcatc gcaacggatg gatacttcat agatatgact gaaacacaga ctctagaagt
    51721 atcaagcctt tttgaagata caacggcgat tttaaaagat attgcagggg tagaatcttc
    51781 tgtctaccaa caacaggatt atacaaaaac taattttgtt caggccccaa ctggagaatt
    51841 cagcattgtg tttcatgttg ggggggcaga cgtagagatt gagttatacg aggagcgtga
    51901 tctgttttga ttttagcagt cacgcttttt catcgtgatt taatgcttta taacgaacat
    51961 ttattctcta acagttttga atttggtgtg gacgagatca acgaagaggc tagtagcttt
    52021 acgatggata agtacgttcc tgtaaaaaca ggcgattttc tactagcaaa atatattcct
    52081 agtggaaaat ttgcatattt tggtgtaatt acgtcgcagg aagacgaaaa aatcagttgt
    52141 aaaagcttac ttagtttagc tgatagtgag ataccgactg cacgtgtgtc aggagataac
    52201 tacgaagagc atatccggcg gttgattgaa tactatttgc tgaatgatcc aacgaaacaa
    52261 ctaaaagata ttttagacgt caaagcagaa agtgcgacct ctcattcgta tcaagctacc
    52321 gatacgaata aacacaagtt aagtgcgtat attctcaatg gtttcaaaaa atacaacgta
    52381 aaatggtatt tcaagggaat tcaaaacaga aaaatttata caggcatacg tgctgtaaat
    52441 gaatcaattt acattaaaga caattcttct gaatttagcg attgggatgt gtttgttcag
    52501 gcgccgggcg ctggaaacga gaacaagcta ttaatcgttg ataaagcaat gaaagatata
    52561 gagaaaccga taatactgtc aacatggtat ttggacgaag aaaacaattt gacacaagat
    52621 ggatcgaaag aaaatatcac gaaaccaact gtcaatttgg ttaatatcta cgatcaaacc
    52681 gcagaagata aagcatccta cgaagatgtg gcaaaatcag agttgaaagg caatacgtat
    52741 tcgcatgaaa tcaaagtgaa cgttgtaaga aatgcaaaaa atttgaatgt cgaaacgatt
    52801 gaaacaggga tgtttgccac gatttcttat aaaggaaaga tatacaagtc ggttttgaca
    52861 gcttggcgaa tatcaagtga taaggagttt gtggaattga ctttcggaaa cataagaagt
    52921 cgttttatgg attattttga agataatggg ggataaaaaa tggttagcaa tgtggatgga
    52981 tatcaatttg aaaacgtgaa agtaagcgca gaaaatgatg ctagacttta tcacgtttta
    53041 tataatcgga aaaatcaggt tattgatggt tacgatcagt ctatgaattt atcttcaagc
    53101 ggattaacag taaaagttgc tgcaggagca gcgattattc aaggtcgtat ggtcgttgtt
    53161 cgacaagaag aaagtataac agttccagca aactcaagtg gttatatagc attaacagtg
    53221 gatttgacac aagaagttat acctggatct attcttccag aatcggaaga atatgaatgg
    53281 actaataatc aagtcaagct agaatttata acaaaagtta taaaaggtaa tttaaacaat
    53341 ggtgataagg tctataattt accactatgc tcagttaatt ctactggatc aactgtttca
    53401 atctcaaaga tatcggatag ttacgagctg actctctcta aaggagaaat tttgtggagg
    53461 gggactgcgt taatgcatga tactcaaact gtccaacctt caaaaaaaat ttggcagaca
    53521 gtttcaggtt ttttgttagt atggctccca tacgaaaacg ggcaagcaat tgaggataga
    53581 tatgtaacta cgccttttta taaggagcgt gtaactttta ctaatgtttt aggagaaatt
    53641 gtttcaggat ttgatgacta tcacaaaaag tggtttagta aacgaataaa ctataattcg
    53701 aatactaata tatttacagg tgcagcaagc aatgcaagtg gagataacgc aaatatggtg
    53761 cttaggtata tagtttcttt ttagttagga ggtatggaag agtggcaaat ttagaaatta
    53821 aattatctgc aaataagaga cagccttatc tacgtcaccg tgttgttggt agagttggtg
    53881 atggggggct cacaacaatc aatgtacaac ttcttgagga agatgaaatt acaccttttg
    53941 taattaatct aaacggtact ttgaaatttg tgggcgaagt ttcaaacggt aactataccg
    54001 agggagaacc agaaataatc gattcgacta atgggttaat tagttacacg ttcactaagt
    54061 caaatttcag cacgagtcat caattcaaac aagcatattt tgaatatgta gatcctaacg
    54121 gcaaaaaagt aacttttcag aacttcatca tagacgtgtt agaacgagtg gatattaatt
    54181 cggagcaagc gaaatactat atttcttcat tggaaaaatt acagagtgaa atgcaaacca
    54241 ctttcaatca gttcattagt gataaacagg tccaatacga tcaaatctac tcgaaatata
    54301 acgaattagt aagattgata aatgaatcag ataagcaagt aaatgatcgt attgatcaaa
    54361 ccaatcagca aatcggcgat ctcggcaagc tgaaaaagat gtacagtaac agcatcgact
    54421 ttgggggcta tgattattcg gggaatccga atttgttaag taagctatca tacgacttaa
    54481 ttgaaaatca aaatacttca gctggaacac tttctaaggg tgaaaactcg tttaaatata
    54541 ataagatatc agctgaagtg gaaggtggag tagagttata ttataaacga agaggtatag
    54601 ctaactggtt accctctaat aaaacgcttg taatgactgt taagcttaga gctggagcgg
    54661 actatagtcc agttgacgga aaacttatac tgattagata taggtatgtt gacagtggaa
    54721 ctggcaagat tgttttagac ttacctatta acagtaattc gataactcag gaatggaaag
    54781 agtttagtat tactggaact actccaacat ttagcccaca agcataccat ccttggatac
    54841 aatttagggc tcaagatggg gtacttgggg aaatagaaat gagctatgac atcaaaatcg
    54901 aagaaggctc tacagctaca ccatatcagc ctaacttact tgatgactct tactggctag
    54961 gtaaaacacc gttgggtgag aatttgataa cgaatgccaa gttcccaatt ataacacgaa
    55021 gcaatcctat ttctggtttt gatatttcag aagaattaat aataggtgaa acttatacgg
    55081 tatctttaaa agggactaag ccagctacta aagaattcca cttatactac ggagaagcaa
    55141 attaccagca gttgcagtat caggcaactt tacttcctgt ggaaggatta acagatgtgt
    55201 ggagtgccac ttttacagcc agaaatacta gtgaaacgac aaatttactt agagtagcgt
    55261 tatggcaaaa acctaattct gcaacatatg atactgttca aattgactgg ctcaagattg
    55321 aaaaaggcga cacacgcaca ccaaatatca gtcagtttaa atactttggt gaaggcttga
    55381 aagacagtaa caatccgaac gactacagct gggatgtcac accagagtat actgaaaaaa
    55441 gcttgaataa tacggttagt ctgactgaac cgcaatccat cgaaggattg aaaaatttcg
    55501 aagatggtat tcaatcaaaa ggaaaatctg tattgacatc agacgacaac aaatatgaag
    55561 tcgtaacctt aacagttaca aacgggaata ccggatcagc aaagctttat cgtgaaggaa
    55621 aaaccgtcac tatttatttt tttgcgttga atgggaaaag cagtggtgga aatgattcaa
    55681 cgatactaac aattccagaa ggctatcggc caccaattag ttttgagcaa ctggttggct
    55741 cgatagaccg ttctactttg aacagtgctc agttatctat tggtgcagat ggagccatta
    55801 aatggcgaag aaactcaagt tatggatcgg attatacctt tgcaattact tacacgattt
    55861 agaaaggcgt gaatcgatat gaaggcagca tatagaccaa ttgaacctta cggattcgag
    55921 caaatcattg tgaatgatga agaacattta ccggaagaat gcacagaagt cgaaccaccg
    55981 attccaaatt ggaaaccgaa attcaattac tgggagggaa ataaatgaaa aacatttgga
    56041 aatatggccg tactggcgga gagtatgcag gaaaagtatt ggacgacatg cttgtatccg
    56101 ttccttacac agatcagcca ccgctcgaag gggttcgttc agatggcgaa ccgctaacga
    56161 ttgctgatca gatgtttgat cctaaattga accaatggat tattttagcg aacgcgttag
    56221 atcacaacga tttaaacaat ctcaaagcga tgtacgaggc tcttgaacat gaaaacggca
    56281 acctaaaaca gctaaatgcc aaactcatgc taagcgatgt agcgattaaa caggaaaata
    56341 ctgcattgaa agaaaaagct gacagtttag cacaaatcaa ttcaaaaatg atgcttgctt
    56401 cgttacaaaa tagcaaagac atttcagaaa ttaaagagca actaaatcca gcttcaaagg
    56461 gaggtgagta gtatgtttag ttttagcgat gtgaaaatga tgtatgattg gggctgtttt
    56521 actgacgatc aagttcgtct attcgttcca ctatgtatca cagatgaaga agcagataga
    56581 attattagca aagaagagag cgcatcttaa gtgatgcgtt tttttgttgg aaagttggtg
    56641 gaacatgaaa gaagaagcgc tccaagacgt tgtggagaga ttagtaagaa ttgaaacaaa
    56701 attagacaac tacgaatcac ttagagaaaa agctgatagt gcaaaagatt tggcagacaa
    56761 agcctactca gtagcgctaa acaacgcaga agatatcaaa gaaatgaaag ccaataataa
    56821 atggtcgtgg ggttacatga tcggtttagg cattacgatc attggctatt tcttgactaa
    56881 attgtaaagg agttaagaag aaatgatttt acccgataag tattatcaag tcattaaatg
    56941 gacggtttta acagtattac cagctgcatc tgttttagta gccacgttag gaaaagcgta
    57001 tggatggaat ggaacagata tgacagtact cactatcaat gcagtagcaa catttttagg
    57061 cgttatcact ggtgtgtctg catataactt aaaaaaatag gaggaaaaaa atgaagaaga
    57121 aaatcatttt atcattgagc ctaataatgg ctcttttttt attgccaatt aacgggttcg
    57181 cctatacgat caacaatgaa tttaatttag gtgtaaatga aggtagctca caagtagcaa
    57241 ataaccaata catcctattg catgaaacag caaacgaaac tgcgacagga cgaaatgaag
    57301 cgcagtacat gaaacgttct tggtacaatg cctatacagc gtatattgtt ggcgatggtg
    57361 ggatcgttta ccaagttgga caacctggtt atgtacaata tggcgctggt tcatatgcta
    57421 atgcaaatag tcctgtgcaa atcgaactgc aacatacgca cgataaagta acgtttgaaa
    57481 agaactacaa ggcatatgtt gaattagcga gagattccgc taataaatac ggtattccac
    57541 ttacattaga cacaccgtat aatcaaccag gaatcaaatc acatttatgg gtaactcaaa
    57601 acatttgggg cgatcacact gatccgtatg gatacttatc agaaatgggt gtaagtaagg
    57661 aaaaactggc ttacgattta gctcatggat ttacagacga aaacccaacg acttctgaga
    57721 acaagcctgt cattgatcca acccgagcag gtgcagcaaa tccaacttta tcagatggaa
    57781 cgaaccattc tcacattgat cagtttggag aaatcgaaaa tgcgaactta cacgtcgctg
    57841 gatggcacat cgctaactat aaatacgagt atattttcat tatggactac aatactggga
    57901 aagaattagc tcgagtaaga gctgatggaa tttatagacc ggatgtaaat caagcttata
    57961 atacttctgg aaacgttggc tatcatgtat cttttaacat gcgcaatttc cctaataaga
    58021 aagtatacgt catgatgcgg gcaacgaatg atccagaagg aaacactaaa ggcggtgcgc
    58081 aagatttcca tgacaaacgt tggtatttaa atattcctaa acgataaaaa tagctcctcg
    58141 ttgaggagca gtacatagaa ttgaaaatta acgttaaatc aaaaaaatat ttacaagatg
    58201 tttatttaag tagataataa aagaaaaaag caggtgaaat tgtgacagca gaaattggta
    58261 ttatgaataa aagtggaatc gtgttagctt cagattcagc atctacgatt ggagatagca
    58321 aagtatataa tactgcaaaa aaattattta ctttggattc catgcattct gtaggtatca
    58381 tgatatatgg taatgctgaa tttaatggta ttccttggga aataatcatt actcaatata
    58441 agaaaagcat tggtagttct gtttttaata ctttggaaga atacgcggat aattttatag
    58501 aatttgtcaa aacagcatct tttattagaa gtgaacagac cgagcaagaa caaatgattg
    58561 gggtttttca aaaaataatt tcaggtttat ttgagagtat agaagtagat attaactttt
    58621 taataagcca aggtacacaa atagacaaag atgtcctagt gaagttatta caaacaaaaa
    58681 tgaataccaa tttgtctcaa cagtcccaga cattcatttt agatattgaa aaggcgttat
    58741 ttcttagtaa ctatggagaa atattaaaag atattcttaa tagtatttct acaatggaag
    58801 gtgtttcaga agctatatca gaggaaattc aaagttacgt ttatgaaatt ataattagag
    58861 acgatgttta ttcttcccct acaggaatag ttatagcagg atatggaaga atggatattt
    58921 tccctaaact atattcttat aatatgtttg gatttgttat gaatattctg aaatactcag
    58981 aatatgaaag tgctcaaata ggaaatgaca atggttcatt aagatctact atattaccat
    59041 ttgctcagtc tgatgtggta aatactgtag ttcaaggggt agatccacaa attactaatt
    59101 atctgtcgag tcaagtcgat agttttgacg ataacggaaa aaatacctat attaatatta
    59161 taaagaatat ttcagagttt caacaaaatc aattcatatt tccgttacta aacatgattg
    59221 ctttactccc agttgaagaa acagcaatta tcgcagagac tttattaaat ttaaccagtt
    59281 ttaaacgaaa atatactact tctgtagaaa cagtaggtgg cccaattgat gttttagcta
    59341 ttacacctaa tgatggccct atttggatta aaagaaaaca ttattttgat attgataata
    59401 atattggata cagattgcga aaggagagag cgaatgatta caataattaa acctcaggaa
    59461 aaatcccctg ttttgaaatt taatgagagc aatctagccc ataataaaat gagtataact
    59521 gatacagtat ttgatgtaat ggttgagcaa caaaaaaaaa ttaataataa caatagacaa
    59581 agaaaagaaa ttaaccaata ttaatgtttt ttctgttgcg ttttttgcag tcataattta
    59641 aaacctcgct caatcttggg cgaggtcttt ttttattttt ctgaaaagtt aaagtaaata
    59701 atagcataaa ataatatatt ttacaaagaa taagtacaat ctagtttttt gctattaaat
    59761 gtgtaataat aaatatgcca tcacaacatg aagaatgaaa tccattatta tctagtctag
    59821 tccattcttt ttgtttgcag tatttgtgat ggctttccgt acccttagct cagttggtca
    59881 gagcagacgg ctcataaccg tccggtcgta ggttcgagtc ctacagggta cattaacgta
    59941 gccatttgaa tcgttctgtg ttagaatttt tttgaagagt attatacaag ctaaagcttt
    60001 tcttcattgc cactcaaatg agtggctttt ttatgtatcc ttttatggat taatgaaagg
    60061 atgtttcaca tagttatact tctgtatatt tgaaaagttt tactttgatt tttaaataga
    60121 aagacatttg ggttaaattg tgagataata ataaagaaga gtttaaagcg caccccaaac
    60181 cacttcccca taagtgtgtt acgctttaaa ctcttttata tttgaagcca ttaaaaagca
    60241 taccatattt ttgaaaaaaa gtgagaaaaa aggcttacaa ttggagtggt agttaattag
    60301 tgacttattt ttgattttat agcactgata ctataaaata tagatatcat catattacac
    60361 aatcttaata ctaacttaaa aaatatctcc tttcacaagt atggtgataa aattcgttcc
    60421 gggctacctt tttaggtagc ctactttaat ctttatacct ttctggatca acgaaagtat
    60481 actttatata gtcataacgc cgatgatcgc tacgtgcgtc cggcacgtca gtc
//
LOCUS       DASHRQ010000011        60248 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.011, whole genome shotgun sequence.
ACCESSION   DASHRQ010000011 DASHRQ010000000
VERSION     DASHRQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.011"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(20..1039)
                     /locus_tag="U2M88_000755"
     CDS             complement(20..1039)
                     /locus_tag="U2M88_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8373156.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1061..2068)
                     /locus_tag="U2M88_000756"
     CDS             complement(1061..2068)
                     /locus_tag="U2M88_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEM8373157.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2061..2726)
                     /gene="pgmB"
                     /locus_tag="U2M88_000757"
     CDS             complement(2061..2726)
                     /gene="pgmB"
                     /locus_tag="U2M88_000757"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEM8373158.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2719..5013)
                     /locus_tag="U2M88_000758"
     CDS             complement(2719..5013)
                     /locus_tag="U2M88_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEM8373159.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5273..7438
                     /locus_tag="U2M88_000759"
     CDS             5273..7438
                     /locus_tag="U2M88_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM8373160.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7742..8575
                     /locus_tag="U2M88_000760"
     CDS             7742..8575
                     /locus_tag="U2M88_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEM8373161.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8677..10377)
                     /locus_tag="U2M88_000761"
     CDS             complement(8677..10377)
                     /locus_tag="U2M88_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373162.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10884..12044
                     /locus_tag="U2M88_000762"
     CDS             10884..12044
                     /locus_tag="U2M88_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEM8373163.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12060..12503
                     /locus_tag="U2M88_000763"
     CDS             12060..12503
                     /locus_tag="U2M88_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8373164.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(12986..13516)
                     /locus_tag="U2M88_000764"
     CDS             complement(12986..13516)
                     /locus_tag="U2M88_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEM8373165.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13621..14172
                     /locus_tag="U2M88_000765"
     CDS             13621..14172
                     /locus_tag="U2M88_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEM8373166.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14325..14798)
                     /locus_tag="U2M88_000766"
     CDS             complement(14325..14798)
                     /locus_tag="U2M88_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEM8373167.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            14936..16408
                     /locus_tag="U2M88_000767"
     CDS             14936..16408
                     /locus_tag="U2M88_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEM8373168.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16405..17115
                     /locus_tag="U2M88_000768"
     CDS             16405..17115
                     /locus_tag="U2M88_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEM8373169.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            17467..17751
                     /locus_tag="U2M88_000769"
     CDS             17467..17751
                     /locus_tag="U2M88_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEM8373170.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18032..18451)
                     /locus_tag="U2M88_000770"
     CDS             complement(18032..18451)
                     /locus_tag="U2M88_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEM8373171.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(18453..19037)
                     /locus_tag="U2M88_000771"
     CDS             complement(18453..19037)
                     /locus_tag="U2M88_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEM8373172.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNQHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(19084..20559)
                     /locus_tag="U2M88_000772"
     CDS             complement(19084..20559)
                     /locus_tag="U2M88_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEM8373173.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            20740..21051
                     /locus_tag="U2M88_000773"
     CDS             20740..21051
                     /locus_tag="U2M88_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373174.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(21166..23502)
                     /locus_tag="U2M88_000774"
     CDS             complement(21166..23502)
                     /locus_tag="U2M88_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8373175.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            23644..24312
                     /locus_tag="U2M88_000775"
     CDS             23644..24312
                     /locus_tag="U2M88_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8373176.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            24315..25358
                     /locus_tag="U2M88_000776"
     CDS             24315..25358
                     /locus_tag="U2M88_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEM8373177.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(25595..26476)
                     /locus_tag="U2M88_000777"
     CDS             complement(25595..26476)
                     /locus_tag="U2M88_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEM8373178.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            26635..27363
                     /locus_tag="U2M88_000778"
     CDS             26635..27363
                     /locus_tag="U2M88_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEM8373179.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            27519..27836
                     /locus_tag="U2M88_000779"
     CDS             27519..27836
                     /locus_tag="U2M88_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEM8373180.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            27829..28437
                     /locus_tag="U2M88_000780"
     CDS             27829..28437
                     /locus_tag="U2M88_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEM8373181.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            28492..29283
                     /locus_tag="U2M88_000781"
     CDS             28492..29283
                     /locus_tag="U2M88_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEM8373182.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            29421..29909
                     /locus_tag="U2M88_000782"
     CDS             29421..29909
                     /locus_tag="U2M88_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEM8373183.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            29960..30832
                     /locus_tag="U2M88_000783"
     CDS             29960..30832
                     /locus_tag="U2M88_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEM8373184.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            31008..31370
                     /locus_tag="U2M88_000784"
     CDS             31008..31370
                     /locus_tag="U2M88_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEM8373185.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            31379..32131
                     /locus_tag="U2M88_000785"
     CDS             31379..32131
                     /locus_tag="U2M88_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8373186.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDVGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            32145..33947
                     /locus_tag="U2M88_000786"
     CDS             32145..33947
                     /locus_tag="U2M88_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8373187.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            34224..35264
                     /locus_tag="U2M88_000787"
     CDS             34224..35264
                     /locus_tag="U2M88_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEM8373188.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(35316..37460)
                     /locus_tag="U2M88_000788"
     CDS             complement(35316..37460)
                     /locus_tag="U2M88_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEM8373189.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(37817..39190)
                     /locus_tag="U2M88_000789"
     CDS             complement(37817..39190)
                     /locus_tag="U2M88_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM8373190.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(39190..39585)
                     /gene="rbsD"
                     /locus_tag="U2M88_000790"
     CDS             complement(39190..39585)
                     /gene="rbsD"
                     /locus_tag="U2M88_000790"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEM8373191.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(39613..40530)
                     /gene="rbsK"
                     /locus_tag="U2M88_000791"
     CDS             complement(39613..40530)
                     /gene="rbsK"
                     /locus_tag="U2M88_000791"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEM8373192.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(40638..41651)
                     /locus_tag="U2M88_000792"
     CDS             complement(40638..41651)
                     /locus_tag="U2M88_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEM8373193.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(41732..42331)
                     /locus_tag="U2M88_000793"
                     /pseudo
     CDS             complement(41732..42331)
                     /locus_tag="U2M88_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
     gene            42517..43218
                     /locus_tag="U2M88_000794"
     CDS             42517..43218
                     /locus_tag="U2M88_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8373194.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            43228..45939
                     /locus_tag="U2M88_000795"
     CDS             43228..45939
                     /locus_tag="U2M88_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8373195.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            46390..48018
                     /gene="treC"
                     /locus_tag="U2M88_000796"
     CDS             46390..48018
                     /gene="treC"
                     /locus_tag="U2M88_000796"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEM8373196.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(48078..49259)
                     /locus_tag="U2M88_000797"
     CDS             complement(48078..49259)
                     /locus_tag="U2M88_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEM8373197.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            49511..51034
                     /locus_tag="U2M88_000798"
     CDS             49511..51034
                     /locus_tag="U2M88_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEM8373198.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(51103..51831)
                     /locus_tag="U2M88_000799"
     CDS             complement(51103..51831)
                     /locus_tag="U2M88_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8373199.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(51844..53124)
                     /locus_tag="U2M88_000800"
     CDS             complement(51844..53124)
                     /locus_tag="U2M88_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEM8373200.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(53265..53867)
                     /locus_tag="U2M88_000801"
     CDS             complement(53265..53867)
                     /locus_tag="U2M88_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEM8373201.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            54112..54318
                     /locus_tag="U2M88_000802"
     CDS             54112..54318
                     /locus_tag="U2M88_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373202.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            54630..55349
                     /locus_tag="U2M88_000803"
     CDS             54630..55349
                     /locus_tag="U2M88_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEM8373203.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(55430..56113)
                     /locus_tag="U2M88_000804"
     CDS             complement(55430..56113)
                     /locus_tag="U2M88_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEM8373204.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            56331..58517
                     /locus_tag="U2M88_000805"
     CDS             56331..58517
                     /locus_tag="U2M88_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEM8373205.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            58863..59273
                     /gene="spx"
                     /locus_tag="U2M88_000806"
     CDS             58863..59273
                     /gene="spx"
                     /locus_tag="U2M88_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEM8373206.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            59287..59643
                     /locus_tag="U2M88_000807"
     CDS             59287..59643
                     /locus_tag="U2M88_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEM8373207.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT        19384 a        10669 c        12140 g        18055 t
ORIGIN      
        1 tatttagtga gtcagctatt tagtatgggt tttctaaagg aacgactgtc tctcttttga
       61 tcaagcgatg agggatgacc aaacgtacgc cactgctcaa ttcttcatgg atcaattcgc
      121 gcagcttttg catagcgaca cgtcctaatt cggagatatc gatatcgata cttgtaaggt
      181 aagggtgtgt taatgttgag aagatcgagt tgttgaagct aatgacggag agtgtctctg
      241 gaatgacata gccatacatt tgcgccaact gcatcgtgcg aagggcgaag atatcgtcaa
      301 tgaccaccaa tgctgtggct tcgttttctt tcaacgtttt ctcaaaggac agataatcct
      361 ccggatgctc tagtactaca ctggataacg caggaagacc agccatcatc attgcttttt
      421 gataaccgaa atatcgttcg aaatacaaac tttcatgagt cgtgtttgtc acaaacagga
      481 tattcttatg tccattctgg atcaaaaatt ctgttgcttg tttacctaga agctggttat
      541 cattatctac ataaacgatt tcgttctcat gatgataagg ctgtccgatc aaagtgaaag
      601 ggatgtggtt ttggtataaa taatcaatga cagtatcttg actatctgaa tacactagaa
      661 taaaaccatc tacttgtttt tgtaaatgca tacgctggac gttctctaac agcaattcaa
      721 aatttttagc agtagcgatt gctgtagtca tatcataatt ccgcgcttct tcgttgattg
      781 cttccatgat ctcaagataa aatgggttac cgatccgctc ttttgaatca agagggggta
      841 agattacgcc gatggcactg gaaatgcgtt ttcctagatt gcgggcagtc atgttaggaa
      901 cataacctag ttcatccata atttttcgga ctttcttctt tgttgtctcg gaaatactag
      961 gatgatcgtt gatgacacga gagacggtcg aggtggcaac accggctttt ttcgccacat
     1021 cttttacggt aatagccatg ataaaaacac ctttctcttg ttattgtgca gagaaccgat
     1081 aaatcgtctg actgatccgt tcttgaccag gatggaggtt gatcgatcca aatttgggga
     1141 aatgaacgat atcagggatt tcttgcggtt cgattgctaa tccgtagttt gaatgcatct
     1201 gttttccatt gacagagaaa tcagcttcga atccagtcgt agaaaataat accatacttt
     1261 tattagaagt tgctatagtc atttggcgac cagaatgctt ctcgaataat tgtaaggaag
     1321 ttttggaaag cctaggatga tgcaaaagaa atacatcatc tagtccatct ggataacatg
     1381 caaggatctc ttgcagtgtt ggggattttc taaagtcgat tggcagtttt tctttttttg
     1441 tccaatttcc agtaggcagt ttttcttgat ctaattcaag cacaccatct aaaaagaccg
     1501 ttaactgatg ttcatagatg tctcttgttc catttcctga aaggttgaag taggcatggt
     1561 tggtcggatt gcagatggtt tcaacaaggc tactagcaga ggttgtcatc tctaattcgt
     1621 tttttgttaa acggtaagtg atcgtagcag tgattggtcc tggataggta gaaaattcat
     1681 cttttaagta aaagaccaca cctattgatg atggagtttt gaaaacttct acttcccaaa
     1741 attggaaaga ccagcctttc gtgccaccgt ggatatggtg tgatccgcag tttttctcca
     1801 atctatgctc accccaagaa gcatcccgga tccttccggc tacaggtccg acagttgctc
     1861 caaaaaagct tttgtctgtt agaacatctt cgaaacgatc gtaagacaat aaaacatttt
     1921 ctgattttcc ttctttgtct ggtgcgaaga tttggtgcag gcgggcaccg taattgagaa
     1981 gaacgacttc taagtagtcg ttcttcaatg tgattttctt tatttcagtc tctggaactg
     2041 gtacgacgga gatctctgga ttatccatgg tcatgccact tttctttcaa gtaatcgatc
     2101 gtaagaaaag tcgtatcagg gacaagcggg atctcctctc caaggtcttc agcacgtccc
     2161 acgccaattg gttgagcgcc acttgagagg atggcttgga taccggcttg cgcgtcttcg
     2221 atcccgatac attcttctgc ttttagtccg acagcttttg ctgcaaggag gaagatatct
     2281 ggcgcaggtt ttccgtttgt tgcttctgcc gggtcggcaa tagcatcaaa gtaaggtgta
     2341 agacccattt tttcaagtag gaatggcccg tttttacttg cagaagcaag agcaatcttg
     2401 attttttctt cttttaaaga atccagtaaa ggcacgaccc cggggaagac atcttttggt
     2461 tcgatcgtct ggatcatctc taaataataa tcgttttttc ttttcgctaa ttctgcaaat
     2521 tcttcctttg aaaaagagtg ttcttttccg ccataagcta aaagcaaaga gagtgagtct
     2581 tctcggctga cgccttttag ctgttcgttg aatgtgtggt cgatcgtaat gccgatttcc
     2641 tgaccaagct ttcgccaagc acgataatgg aattcagcag tatcggtaat gacaccgtct
     2701 aaatcaaaca ataccccttt aaacatttgc ttcctccttt cttagcgtta tttctagacg
     2761 atccgctaaa gaataagact gttcatagac gtaaatttct agaggagaac cttctaataa
     2821 ctcgaaagta acttgttcat ctgtgactga gatgaacaat aaacgtccgc gataattgat
     2881 atggaaagaa taagctttcc aagtttttgg cagaaatggt gcgaatctca actgatcatg
     2941 ggcagttttc atttgagcaa atccttggac aatcgacaac cagctaccag tcatggaagt
     3001 gatgtgcaag ccgtcttctg tgtcgttatt gtaattatcc aaatccaagc gagctgttcg
     3061 ctggtacatt tctacagctt tgctttccat tcctaattct gctgctaaaa tcgaatggat
     3121 acttggcgaa agggaagact catggacagt cattggttca tagaaatcaa agtttttttg
     3181 cttttcttct tttgtaaaag tatcattgaa gaaataaatc ccctgtagta catctgcctg
     3241 cttgatgaag caagatctaa ggattttgtc ccaagaccaa tgctgattca gaggaagatc
     3301 gcttgtggga atatcggata caggcatgag atctttatct aagaatgtgt catgctggac
     3361 aaagattcct aattcttcgt caactgggaa gtacatgttc tctgcgatat ctttccattt
     3421 ggctagttct tcttcgctga tagtgatagc agcttctttt tgataagtta aataattttc
     3481 tcgcgtgtaa cgcaagaccc aagctgcgat cgtatttgta taccaattgt tattgatatt
     3541 gttctcatat tcgtttgggc cggtcacacc gtggatcatg tatttatttg ctcgttttga
     3601 aaaatggaca cggtctgccc agaagcgtgc gatttctgct aaaacttcca gtccttcatt
     3661 tttcaaatat tccgtatcgc cggtgtaatt cgtataattg tagattgcat aggcaatcgc
     3721 gccatttcgg tgaatctcct cgaatgtgat ttcccactcg ttatggcatt cgacaccagt
     3781 gaaggtgacc ataggataaa gcgcgccttt taatccttgt tgctgagcgt tgtggattgc
     3841 ttgcggcagt tgattatgcc gatatttcag cagattctta gttacttcag gtttggctaa
     3901 agcaagataa agagggacag catacgcttc tgtatcccag taagtggctc cgccgtattt
     3961 ttctcctgtg aatcctttgg ggccgatatt caatcgttca tcttcgccgt agtaagtaga
     4021 gaatagttgg aacaaattga atcgaatacc ttgttgtgct tcttcgtctc cttcgatgac
     4081 cacatcggca agcttccatc gttctgccca agccgctgtt tgctcttctt ttgcttggat
     4141 ataattgttt gtctggttcg ttaattctga gacagctttt tctgcttgct gcgcttctgg
     4201 aatatctcta ctagtaagaa cgataatttc tttttccact gaccatgtct caccttgaga
     4261 taattctgca gacaagcaac catgtaatgc taaagtatct tcgtgatagt cagggatgac
     4321 tgattttcca ttaacgaaat ggcgcattgc tgcagtaaca gtaaattgtt caatcccaaa
     4381 gttgtttgga atagttttag tcgtcaaata gccgatttct tctatagttc cacgatcaat
     4441 ctcattccaa aaatgttcat catagttgct gtcttcgttt tgaacatttc catccaattt
     4501 tgatacgagt ttgactgttc cgctaccttc aagcatttct atagttaaat agatataagc
     4561 tgattctttt ttgacaatgc tcagaaaacg ttcgaaggaa atgcgtactt tattagtaga
     4621 agtagtgatt gtgaatgtac gagataatac accatttttc atatctaatt cccagtaaaa
     4681 gtcttctggt tccaaagaag ccaagtcgat cagctgatcg ttgatcgaaa tatctatcgc
     4741 gataaagttc aaggcgttga tcacttttcc aaagtattct ggataaccat ttttccacca
     4801 gccgacacgt gtcttatcgg gataccagac acccgccaaa taagtacctt gatggtggtc
     4861 ccctgagtaa gtttcttcaa agttcccgcg catccccata taaccgtttc caatacttgt
     4921 taaagattct tgcagtcgaa gatttttttt atctaattgt gtcgtgctga ttttccatgg
     4981 atcaatagca aatagtcgtt tgatctgttt catttttctc ctcctaatga gctgttttat
     5041 acgttcataa taaacgcaac cgattgcaca agtcaataga caaaagaaaa tatttcatag
     5101 aaaaaagctt aaaaaaataa atttttagga gaaaattata gaagggaaca cacgtattat
     5161 aggttttata gaatggtata gacagaaaaa taaaaaataa agcatttaca aaaaagaaaa
     5221 cgcacgctat aatacgttta acgaaaccga ttgcgtaaag gagctaaacg gaatgaaaaa
     5281 gatatttagt tttgagtttt ggcaaaaatt tggaaaggct ttaatggttg tggtagcagt
     5341 tatgccggca gctgggttga tgatcagtat agggaaatca attcccttga tcaatcctga
     5401 ttggacgccg cttgtcacaa cgggcggagt aattgaaaat attggttggg caattattgg
     5461 taatcttcat attttatttg ctttagcaat aggaggaagt tgggcaaaag aaagagcagg
     5521 cggagcattt gcagctggga tctcatttat tttaatcaat cggatcactg gggctatctt
     5581 tggtgtcaca agtgacatgc ttgcgaatga agatgcattt acccacactc tgttcggtac
     5641 gaagatcatg gttaaaggat tttttacaag tgtattggaa gcaccagctt tgaatatggg
     5701 agtattcgta gggatcatcg ctggatttgt cggggcaatg gcttataata agtactataa
     5761 ttatagaaaa ttgccggatg ctttgtcatt tttcaatgga aagcgctttg ttccgtttgt
     5821 tgttatctta tggtcaacaa tcgtggcatt gattcttgct gtggtgtggc caaacgtaca
     5881 ggctggaatc aataattttg gtttatggat tgcagaatca caagaaagcg caccgattct
     5941 agcaccgttt ttatttggaa cattagaacg tttactattg ccatttggat tgcatcatat
     6001 gctgacgatt cctatcaact atacacagct tggcggatcg tatgagattt tatcaggcgc
     6061 acaggcgggg acacaagtat ttgggcaaga tccgttatgg ttggcttggg ccactgattt
     6121 agtgaatctt aaaggtgcag gggatatgtc gcaatatgag tttgtgttgc ataactggac
     6181 gccggctcgt ttcaaagtag gacaaatgat tggttcttca ggtatcttga tgggaatggc
     6241 ttttgcgatg tatcgtaatg tcgatcctga taagaaagca agatataaat caatgtattt
     6301 ttcagcagcg ttggctgtct ttctaacagg tgtaacagaa ccattggagt tcatgtttat
     6361 gtttgctgct gtgccattat atgtcattta ttcagtgatc caaggggctg catttgcgat
     6421 ggcagatatc ttgccattgc gggtccattc atttgggaat atcgaattgc tgactcggac
     6481 accgttagct atcaaagcgg gtctaggtgg agatcttatc aacttcgtgt tgtgtgtgat
     6541 cgcttttggt gttgttacgt atttcttagc aaatttcttg atcaaaaaat tcaactttgc
     6601 tacaccggga cgtaacggaa attacgacaa tgacagtgaa gaaaccgttg tatctaacag
     6661 cggaacagga acagcagatc agcaagtggt ccagatcatt catttgctgg gaggaaaaga
     6721 aaatatcaaa gatgtcgatg cttgtatgac tcggttacgc gtaagtgtaa atgatcgaga
     6781 aaaagtaggg actgaagaag cctggaaacg agcaggggcg atgggattga tcgtcaaaga
     6841 caatggagtc caagcagttt atggaccaaa agcagatgtc ttgaaatcag atatcgaaga
     6901 tctgctgcaa tcaggagcag agattccaga gccagaaatg gtttctacag gaaatggaca
     6961 agcaggtagt aagcaatact taggtataga acaagaacta gtttccgcag cttctggaga
     7021 agtgattcct ttatctgaag taaaagatcc ggtattctca caaaaaatga tgggtgacgg
     7081 ctttgctgtt attccaacat caagagaagt tgttgcgccg atcgctggaa aagtgacaag
     7141 tattttccct agcaaacatg caattgggct ggaaacgaaa gatggtatcg aagttttgat
     7201 ccatatggga atcgatacag tccaaatgaa tcagccagct tttgagattg ctgtcaaaga
     7261 aggccaagaa gtaggaacag gaacgaaatt agctgagatg gatcttgatg tgatccaaaa
     7321 cgaagaaaaa gatccaacga tcatgatcgt atttacaaac gataaagtag aagaagtagt
     7381 gatcaagcaa ttaggtacag tgacagctgg taaagtgatc ggctccatta aaatgtaagg
     7441 agaggttgtg aaaaagctgt cctgcatcaa gtcataagaa cagccaaaca aaaatagctc
     7501 ctcctatttt tcgtttggtt gggcttatta ccgagaagca gcagtttgaa tactgtttag
     7561 tggagaggtc gtgaaaaagc tgccctgcac caagtgataa aaacagccaa acaaaaaata
     7621 gctcctggtc cattgagctt attactgagg agttagcagg agaacactat ttgctggaga
     7681 ggttgtgaca aaaattttgt cacaaccctt ttgtttcgaa atgaagacaa ggagaaaacc
     7741 aatgaaattc atgacactca acacgcatag ctggcttgag ccagaaccag agaaaaaatt
     7801 gcaagaatta gcagataaga tcctgttaga agattatgaa gtgattgctt tacaagaaat
     7861 caaccaatta ctggaatctg aagaagtaga acctgcgaag ctaatgaagt tctgtccggt
     7921 aaagaatcaa gtacctatcc gtaaagataa ttttgcctac cgtttagttc agcttttaaa
     7981 ggaacacgga aaagagtatt tttggagttg ggaaatgagc cacatcggtt atgataaata
     8041 tgaagaagga aatgcactgc ttacgaaaaa aacattggaa agtcaggtcc ttaccgtctc
     8101 ccaatttcaa aagaaagaag attatcagac acgcaagatc ttgattggga aaacaaagat
     8161 agatgatcag gacatttttg tctcgagctg ccacttttct tggtggacag ataaaaaaag
     8221 tggcttttat tttgagtgga aaaacttgga aaactacttt ttggaaactc gcgtaccact
     8281 ttttttccta ggtgatttca ataatccagt agactctcaa ggttattata ctgtaagaga
     8341 aagttgcctt cttcttcaag attcgtatgt tgtcgcgaat gaaaaaggaa aagcagctac
     8401 tgtggaaaag aaaatagatg gttgggaaca aaatacagaa aaattgcgta tagattttat
     8461 tttcgtccct gaaggaatgc aggttaaaaa atatcaacgg atttttgatg ggatcgatag
     8521 tccaatcatc agtgatcact acggggtgga aatcgaatta gatgtgaacg aataaaaaat
     8581 ataacatata acataaaaaa actgattaaa ataaaactct ccaaacgatt ataccgtttg
     8641 gagagttttt tgatcggatg tatttatccc ttttgcctaa gcacgtaaaa gcttgtatct
     8701 atgataaata aataaacata gcaaggtaaa ccagccaatc aaagtaagag aaagaatgaa
     8761 atagaaatag attggcgttt gataatgaag cgtgagctcg ttgattcctt tttgctgctt
     8821 cacgattggg gtcccgatat ctgttacttc ataatcagtc aatgtttttc cattttgctg
     8881 tagcacagtc cgatcgtaga cgataacagg gatatgaaca gttttgtccc cagtgttttt
     8941 ccacttgatt gtcaaaagac cgcctggatg tgtttttata aaagttgttt cgttttctag
     9001 tatttcttcg ttataccggt cgtatttatt gtcttttgtc tccttataga ttggcagata
     9061 gtcaggtgtg gttttttgga aaacgaacaa gctggtagac atatctttgc tgaagaatgt
     9121 tttccttgct tcttccggcg tatcgaatag ataagtatgt cgactgacaa atgtttcatt
     9181 ttcccacttt tccagatgct ggtataagtt ctggatcgtc tgtgctgaaa aaataagaat
     9241 gccaaatata cttaaaagac ggaaccactt ttgttcagac cactggttca gtgaaagggc
     9301 aaggtaaagt agaagcaata gcgtaaatgg cacgaaaaat cgaaatggaa actggatcaa
     9361 actaataaaa ggcacatttt tttcgatcaa tgtcgtccat ggaatcaagc tagtagaaag
     9421 aaaaaagaaa acaagacttg tgtacaacac gattttttgg aataaggtgt tcttttgttg
     9481 aaagaaaata aaaagcaaaa aacctgataa tatcaatata ggaaaggcct tagggtaatg
     9541 gagccaataa ctgccatcca gatctatcgc cttttgactc atttctctgt taacaaaggg
     9601 aggaagaatc ttattcccac tgtaaacaat agctaatcct gcccaaatat ttgctgtcag
     9661 aacaaaataa agcaagaccg acttaatcag ttgaagcagt gcttttcctt tttcagtctg
     9721 gttaaaaaag taataaagat aaaaagggat atacatcaaa gctaacatga tcgaagacag
     9781 taaatggatc tgtgtcataa gtcccactga gacagcgact ttgataacag ggaacttttt
     9841 atgaaggacg aaatcagcaa tagggatcat acataaagga taaaaacaag tcccccagct
     9901 agtgaatcct tggttgatag accagtattg aatagaatac gtactcatga ataaaacggc
     9961 tactggtaca cttacccaat ttcgaacatt gacatatctc agtaaaatca gcatagaaaa
    10021 tcctgaaata agataaagaa tgaaatttga aagaatctga taatgaaacc agctacctgc
    10081 aagcaacacg atcaatcctt gtaaataagc gaaaaatggg ccgtaaacgg cattcacgat
    10141 cctgccactt tgctggaatc cataaaggga aggaaaataa tggaaatccc catatttaat
    10201 ttgttgcgcg gcatcataga accgattgta atggaatggg gcatcagcac ccaaaattag
    10261 tttatgtgtg aataaatgag gcatgagaaa tgccaaagca atgaaaaaaa ttgctaaaaa
    10321 tgggccatat tttttcaatt gtcgaactcc tttgcaaact ttagataaat tcatcatata
    10381 tcctatgcga gaggtaggca agaaattaga aaattcatga aaatatttat gaattttaca
    10441 agaaaaacat aagtgaaagt tctaaggaga gaggatgcga cataagtttt gttgcatctc
    10501 cttgttttga aacagaattt ttcttaatac tcgaaacaaa gtagcttttt catgacctct
    10561 cggtaaacgg tgttcaatca gctgcttctc ggtaataagc tcactagcct agaaaatatg
    10621 agaagctatt ttctgactag cgctcttatt actcaaagca taacgcagtt ttcacaacct
    10681 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc caacaaaaaa
    10741 tatgagtagc tatttttttg ggctgtactt attacttgat gcaggacagc tttttcacaa
    10801 cctcatcggc tttcattttc tatttttttc ctgaacccat ataataaaag aaagaacaaa
    10861 aaaacttgca agggaggaca catgtgaagc agagtaagca aaaacagtct cgtcgacttt
    10921 tgtttcgttt gtttttatta atatgtatcg tcgtagggtt actgatcgct ttatatccat
    10981 tttatgtgga ttctttaaat agttttattg atcaaaaacg tatcgaacaa gtacaaaaac
    11041 ggaccgcagc tgaaattgaa gtgcagcgaa agaaaatgga ggagaaaaac caacgattaa
    11101 ctgaccaagg attcaatcct ggtactgatc ctttcaatga aaagaacaga aatgaaagta
    11161 caacatccag ccaattagag gaatggctga tcggaagtat caatatacct aagatccaat
    11221 taaatatttc actttatgat cggctcaatg gtaggatcct tgaaaatgga gcaggcgtct
    11281 tgcaaggaac ctctttcccg cttggtggaa attcgacgca ttcagtgatc tctgctcaca
    11341 gcggtttacc taatcgacgg ttatttaccg aattagatcg tttagaaaac ggagacacgt
    11401 ttattttgac tgttttgggg gagaaattgg cttatcaagt taaaaatata caagtggtac
    11461 tgccagatga cacgtctgtt ctaacgattt cagaaggaga agacttagtt accttgctta
    11521 catgtacgcc gtatatgatc aacacgcatc gtttgctagt gacaggacat cggattcctt
    11581 ataacgaatc ggtaaaaaaa gaagaaggaa aaggaaatca ggaaagaacc atgcggcaat
    11641 ggtttattct tgcaggaacg ataatagctg tagtcatact gttagtgttt atcggacgac
    11701 taatctatca gtatagatta agtaaaaaag tgcttgattt ttcatttatt attagtgatt
    11761 cgtcagggaa accggtaaat ggtagttcat tcattttgaa acataagaaa aaaacactta
    11821 ctagaaatgg tgtgccgttt tcagtacaaa gcgaccatca tgggaaagtc aagctggatc
    11881 aattgcctgg tggaacttat cgaatcgttt cagctgatga tcctaaagtt gctgtttctt
    11941 ttggtattag aaaagtaaaa caagagaaga tatacttttt tgaggggcga aaacttgtaa
    12001 aagagcttca aaaaaatggt ttttatttta aaatcaatga ataagtaagg agagaaaaaa
    12061 tgatccgcac aacaacaaaa ttgtctgcca aagaactgga atctgttcta tcgatttggc
    12121 tgcatgctaa cttggattct catgaattca ttccatcaac ttattgggag aatcaagtag
    12181 aatctgttag gaaagcttta ccagatgcag tgatttactt agcagaaata gatcaagaaa
    12241 ttattggttt tgctggggtg attgatcagt ttatcgcagg aatctttgtg aaaagggaat
    12301 accggcaaca aagaatcggt acgcaactaa tgaaaaaaat aaaagaaacc aataaaacac
    12361 tgactttgac agtctatcaa aaaaatcaat cagctattca tttctatctt tcacaaggtt
    12421 ttacaattca aacacagcaa aaagatgaag cagtaaatga ggaggaactt gtgatgaaat
    12481 ggaatcaaaa cgctgatttt tgatgaacag aataacgaaa taatgctccc agcctaattt
    12541 tttgctggga gcattatttc attttgaagt cactaattta actgtgtgaa tatgcagttg
    12601 ttacaatctt tgataaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg
    12661 caaaatatga ggaactattt ttccatttgt ttctctaata ctcaggtcaa aacgctgatt
    12721 tcacaacctc ttgataaacg gtgttcaaac ggctgcttct cggtaataag cccactagcc
    12781 tagaaaatat gaaaagctat tttctaacta gcgttcttat tactcaaagc ataacgctga
    12841 tttcacaacc tcttgataaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa
    12901 cggcaaaaca tgaggaacta tttttccgtt tctttctcta atactcaggt cagaacgctg
    12961 atttcacaac ctctctttca caaccttatt ttactcgttt ataattttca tcgaagtatt
    13021 ttccgctacg gatatcatct ggcattccat cgtatggttc ttcgctaata acatcttcat
    13081 tgtctatacc tgcatcaccc atataagtga tcgtagcaaa ttcaggaaca agtaattttg
    13141 gataagtttc atatttttca cgagctgctt ccatttctgc gatatgttcg ttttggaaag
    13201 taacagtgat ttctggatgt tcttcaaccc atttagggaa aaagatcatt ccgtgaagtt
    13261 tcttttcatt tggcataaag tccaatgcca cgtctgtccc tgttggctct gtccaagtga
    13321 ttttgaatac gccgtccgtc aatttaacga tatcagcttc ttggtctttt acccaacggc
    13381 ctttgaccat tccgccatgg atacggtaat caacagtatg gtcatttttg gcataccatt
    13441 catattccca accattgtca tacgtataga taaagtgagt tccaataaaa tcttccaatt
    13501 ctttaaattc tttcatgttt aagcatctcc ttataaatta aaacatgtaa taaactacat
    13561 gttttcgatt actcatttat gatagaatgg atttaaagat ttgtcaagga gggaaaactg
    13621 atggcaagaa aaaatacact tcgatatatt ttgttagggt tattaagtaa gaagaagatg
    13681 acaggatatg aattgaatca atcgttcaaa aatgaaatag gagaattttg gcaagcaaaa
    13741 catagtcaga tttatcctga acttgcaaaa atggaagagc aaggaatcat tcagcatcaa
    13801 gtagagatca caggtgaaaa attggaaaag aaagtttatg aattaacaga agaagggaag
    13861 gaacaattgg cagagtggat tcatacaccc accaatgaac taccggttaa tcgagacgaa
    13921 tttgttctga aattgtattt cgtcaaagac gtgaatgatc cggcattagt agaaattatt
    13981 cagcagcagc gtgaccttca tgaagaaaaa cgacttcatc tattagagcg aaaaaaaacg
    14041 atctttccta ctgaaaaaga aaaacatgaa aattacggac actacctgat tttagcgcat
    14101 gcgatcaatc gggaaacaga atatgcaaat tggctaacag atgtattaga agaaagtaac
    14161 cgaaaaaaat aaaaaaatat ccgaacagtg atggagaatt cctttctata tgaaagaaac
    14221 gcagatcacc gttcggatat tcttatgttc aaacaggcct tttttcaagt atttatgtag
    14281 catctactta ttaaaaagct tggtacttag gagattagtg gcgtttagag ttcttttaac
    14341 aactgttcgt agtcagcttt ttcatcgaca ggaaggagct tgtaagttaa ttcgtctgtc
    14401 tcgttgtcat gaatgatgta catcttagca ttctcttctt tgaagttacc gatttgtaaa
    14461 atcgtatagc gttcgtcgat caattcgata tctttgattg cttttgaaaa aactttacgg
    14521 acatggtgca ttttcttttg gcggatttct gccttttctg cagcttttga caaagtaaat
    14581 ccgtcatcta aatatttttt gatcagttct acttttatga ctgtaggtaa acgatatttt
    14641 ctaggacaat tcgcatcagc ttcgatcgaa cgaatgaatc ccttttgttc ccaatacctt
    14701 aattgacgtg gagacacacc tacgatatca cttaattcac tgatgccaac aaccaattga
    14761 tcattttcca acaaacgttt aagggaagcc ccaaccattt ttgtcactcc ttttgtcctc
    14821 ttgcttccct taattatatc cgtattaact tatgtgtcaa gtaggttgac aaatagcgaa
    14881 tttaggacta aagtattcta gtagtttcag aaaaaattca gaaagaagga gaaatatgag
    14941 taacaaccaa cccgtagata tttacggaaa accatataat cggacacttt tagttgttgt
    15001 cttattaatt ggtacatttt gtaccgtttt gaatcagacc ttattgacga cagcttttcc
    15061 tacattgatg aaagctttcg atatttcagc gtctgatgtg caatggttaa cgactggttt
    15121 cttgctagtc aacgggatca tgatcccaat aacagcatgg ctgatcaata aattcagttc
    15181 aagaaattta tatctttctg ccatgacgat ctttttgatc ggaacgatta cttgctttac
    15241 agcacctaat tttagtacat tactaatcgg tcgtttgatc caagcttgcg gtgtgggagt
    15301 atcgatgcca ttattgcaaa atatcatgct atctattttc ccgccagaaa aaagaggatc
    15361 agcaatgggg atgtcaggga tcgttattgg acttgctcct gctttaggcc ctacattatc
    15421 tggttatatc attgataact accattggcg tgacttgttt ggtatggtac tgccaatcgt
    15481 tgttcttgtc ttggttttag cattcttcct aatgaaaagt gttattccat tgtcaaatcc
    15541 tagcattgat attttatccg ctattttatc aacgattgga ttcggtagtt tactttacgg
    15601 attttctagt gtaggagatg acggctgggg aagcttgaaa gtaattggtt tcttagttgt
    15661 tggtgtgatt ttcatcgcct tgttctcatg gcgtcaatta catttggaac atccattctt
    15721 ggaacttcgt gtattcaaat catctacatt cacgattgca gctgttttag ctggtgtaac
    15781 aaatatggcg atggtagggg cagaaatggt cgtaccattg tatattcaaa atattcgtgg
    15841 agaatcagca ttccattctg gcttgatgtt gttgccgggt gcattgatca tgggggctat
    15901 gatgcctgtg acgggtgcga ttttcgataa atacggcgcg aaacgtttag ccatcacagg
    15961 gatgttcatt ttgacagcgg caactgcacc atttgctttc ttgacaaaaa cgactccagt
    16021 tttatatatc gtgatccttt atgcgatccg tatgtttggg atctccatgg tcatgatgcc
    16081 ggtaacgact tctggtatga atgcattgcc aaataacttg atcagtcatg ggacagcggt
    16141 aaataataca ttccgtcaaa tcgcaagctc gattggtaca gctattttga tcagtgtgtt
    16201 gacgaatgtg acaaaagcaa atatcccaag caaatctgta ttggataata cgccattagc
    16261 ttataaagat caggcaatca atgccacact gtcaggttac catgccgcgt ttatcgtagc
    16321 gatcatcttt gggattcttg gctttattat tgccttttta ttgaagaaaa aagaacctgt
    16381 tctaatgaaa gcgggggata atgcatgatt attggagttt tgattgtcag cttgcttgct
    16441 tttgctttaa cgaacgtgtt cgctaaaaaa acatggcaaa cctttttatc tttgattttc
    16501 ggactaatct tcgtcgcttc actaagtttg atcgtcgcta atttgtccaa tcactttgga
    16561 atggaaaaag taacggaaac aaaaacagaa aaaattgttt ccagtgctga ttcccaagga
    16621 gcagatatgc ttttgtataa agctttaggg aatggaaaag aaaaagtgta tctttaccga
    16681 acaaatgaaa aacaagaaaa accaaaagca actggtacgg ataacgaaac aaataaagta
    16741 gaaaagacag acggcgatgc tgaaaaagtg acgaaaacta cttattggga atacaaaaac
    16801 gacatgtata aattctggtt caatattgct gataacaatc acgaatatga cagccgagtg
    16861 aatacattca aaatcccaga gacatgggta gaattaagta cggaccaagc agccaagtta
    16921 gctgaattag tgaagaaaca gcaaagtacg atggaaagtg aagcaaaagc ttatgttcaa
    16981 gacggtatgg taaaagcaat gactgaaaat ccaaaaatga gcaaagcaga gcaagaacaa
    17041 cgtacgaaag ccttagctgc tgaattccaa caacaagcat tcgcaaaatt agtgaaagaa
    17101 gcaaaaggcg aataatattt tgaacaccat gtaggaggtt gtgaaaaagc tgctctgcat
    17161 caagcagtaa gaacaatcag ataaaaatag cactccatat ttttcatctg attgggctta
    17221 gtgccgtaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgagc ctgccgttta
    17281 actctgagtc acaaggagca atttcaaaaa acagcttctc atgtttgttg actattgtga
    17341 cttgtgaccg aggagttggc aggagaacac cgtttattcg aaaaaaggaa ctgcgacaag
    17401 acttttgtca cagttccttc ttttttttgc tatgctatac tagatgtaaa aaataggaga
    17461 aataaaatga gggaaattta ttcttttgaa tcaaaaatct atgcttccga agtcggcaag
    17521 ggcggggcat atattatttt tccgtatgat ataagaaaag aattcggtgc tggaagagtt
    17581 aaagtccaag caacttttga caatgtgccg tatgaaggca gtatcgtgaa tatgggcgtg
    17641 aaaaatccag atggttcagt ctgctacatc cttggactac gaaaagatat tcgtaaaaac
    17701 atcggaaaag aaatcggtga tattgtagca gttacagtga agcaaaaata aaagaggttg
    17761 tgacaaaaga cgtgtcacaa cctcttgata aacggtgttc aaaagccagt ttctcggtag
    17821 taagtcaaat ttatgcaaaa atgtggaaac ccatgtttac aaaatttttc ttactactcg
    17881 aaacgaagcg gctttatcac aacctcttga taaacggcgt tcaatcagct gtccctcggt
    17941 attagctcaa aaacggcaaa atatgagaag ctatttttcc atttttttct ctcatactca
    18001 ggtcataacg ctgatttcac aacctcttct ttcataatgg tttttcacca tctttataat
    18061 aaactggttc tttgatatgg aaatctattt tttccaacca atctaaaatc ttgtaaccta
    18121 actcttctga catcggaaaa gaggcgttga tttgtgcatt ggagatatgg tcaacatgga
    18181 taccggaagt gatcgtacaa cttcccggaa cggcagtttt gatgatcaca gctatttttt
    18241 cggctaagac atcgtcttta tggaaccgac catcgtggct agggaaacgt acagtttgca
    18301 tctctgtatc acgagaaaat gtagtgactg ttccgatatg tggtgtatct ccgcctgtta
    18361 aagtgatcaa taagtcttta ccgattactt taacagcagc ttggatcgtg aaacctagtt
    18421 tggttacttc aactatcgtt tctttttcca tattaatcac cctgccaacg ttttgaatgc
    18481 tcatttacaa tttcaaattg atccaaaagt ttcattgtat gttgattaac aagatcttgg
    18541 atcgtttgag gctgattata aaaagcagga attggtggta tgatttgcac acctagtcgt
    18601 gctaatttag tcaagttttc caagtgaatg gcatttaatg gcgtttcacg aggaacaatg
    18661 actaactttc gttgctcctt cattgttaca tcagcagcac gtgcaatcag gttgtcgcct
    18721 aaaccaacag agatacctgc gactgttttc atacttgccg gtacgatgat cattccatcg
    18781 gtcaaaaatg atccgctggc gacagcagca cctaaatctt tgtttgaata atggtaatca
    18841 aacaggcttt cgaattcagt tagtgataag tcggattcca gctttaagtt ctgttttgcc
    18901 catggactga tgattccgtg tgtttctatc atcggatatt cttttagttt cttcactagg
    18961 ttgatggcat aaatggtgcc tgaagcgcca cttacaccaa tcacgatttt tttcttccgt
    19021 tgtgccaatt gttccatttt ccgcactcct ttttatgatg tcaaaacagt tgcagatcat
    19081 tcattatttc aaatattttt cccaatcttc tacttcttgg aattttgcac gtacaaaatg
    19141 ttctttcata tcgaaaggaa ctgtaccgtc aaagatagtt tttgcactta tcccacggaa
    19201 acgaatagag ccaggattat attctggtaa ttctgatggg tccaatggat ggttcctcat
    19261 tcctgggatc acgatgatat ctttatctcc ttggaaacga gtgttgaaag tccaaactac
    19321 atcgttcatg tcgaaaatat caacatcttc atcgactaaa atgactgtct tcaattcttt
    19381 gaaggctgat aaagcaagaa tagctgcttg acgctgaata ccttcatccg cttcgttttc
    19441 tttacggatt tgcatgatgg tcatcaattt accaccacct gcaggaggat tgtagacatt
    19501 tactactttt cctggaatag ctttatcgac caaggcaaga atacttgctt ctgtaggaat
    19561 accggccata gatacgtgtt cttcactggg accaatcgtt gtttgcatga ttggattgtc
    19621 ttttcggtga gtaactgctt tgactttcac tacttgtaca gctggattag catccccagt
    19681 atagccagga aattctggca ttgcttttcc tgagttcgtg ttgatatctt cttgcattgt
    19741 ttcatttggc atgatttctg cttcaatgat gaattcagca cgagcaatac ccacttcatc
    19801 gactgctata ccatcaacga tttgaatagg ttcgttacgc aaagctcctg cacaccatag
    19861 ttcattgtag ccaagtggag tggtaggtgg ttcgaaagtc gtgccaatgg caattgctgg
    19921 atctaaacca atattgatag tgataggcat tggtttattc aaggcttcga attgtttttg
    19981 gaagtgacca atgtggcgtc cgccaggcat gatgtacata ccgatcgtat ctttatcttc
    20041 tagaaccata cgatggatcg ttacatcgct cattgttttt tctggatctg atcctaagac
    20101 tacacccatt gtgatgtatg gaccagcatc ttcaggagta tttgtaggtg caggcaaaat
    20161 gttgcgaata tcgaagtctg catcggtagc tagatgaacg acttcttgtg ctggagcgtc
    20221 tgctttgtct actttcacag gtttgattgg attttctaca gcgtctttca agaaacgacc
    20281 aagtgttcga taatcatggt gcaaaatttc acctacacgt ttacgactag ccattgttcc
    20341 gatgttcaca cgtgtgccgg ggaaaccttt gatattgttg aaagtcatag ctggtccttc
    20401 ttgagtcgga cgttttactg taccgccagc accgatataa cggtaaacac cagataattc
    20461 tgcttcagga tcgatttcaa tatctgtttc gtgatattga cctggaagtg tttttagttc
    20521 ttcgatgact tttcttaaat catacggttg atctgtcatg agagattcct ccttagttgt
    20581 ctttacctat ctatagtaaa aggtgaaaaa gaggaaaaca attcaacttg ttcggtaaag
    20641 tattctacaa tggaatggat gcgttttttt gataatagaa taaactatga tatacttatc
    20701 actaaaagaa agcgcatact tagtgttgga ggaaggaaag tgaaaaaaat ggaggagtca
    20761 tttcctaaag cagtaaaagt ggaaaatata gcgaatattt tgaaagttac atttgaaaac
    20821 ggagaagtaa aatacgtgaa atctcattgg acagaagaga taaccgatgc cttgcaattt
    20881 ggaaaaaaag gaagaggaaa aagaaaaaat cttctcgcac tttctcgtaa tatgtggatt
    20941 gggactgaag taacgattga agcagatgga acagtattca taaatggaaa agacaggtat
    21001 acgccagaag agttgtggta caaaggaaag aaaagtatac cagaattata aaaaataatt
    21061 aaaaaaaaca gcgcgaaaca aggaacatat tacttcctcg tttcttgctg ttttctacta
    21121 tttcagcgct cttatgactc aaatctaaat gtggttgtca caaccttatt ctgcacgcaa
    21181 agcgatagcg gcgtcttttt tcgctgccat tcgtgcagga agatgaccgc caagcatcgt
    21241 caatatagta gagacgatca ctagaataat cgcatggaca ggatttagtt gagcaacatt
    21301 ttttagatcc gtcatgttgt ataagatgct attgataggg aacgtagcta gccatgcaat
    21361 aaacacacct aatatcccag aagaaatacc taaaatacac gtttctgcgt caaatacgcg
    21421 agtgatgtct tttttacgtg cacctaaagc ttttagtaca ccgatttctt ttgtccgttc
    21481 aatcacagat gtataagtaa tgatactgat catgatcata cttgttacta aagagatccc
    21541 tgcaaaagca ataaggacat aagtgatagc atccatcaat ccgccagtca gttcagtcat
    21601 cgttccagct aaatcagtat aaatgatttt atcttcatct gatttgcctt tattgtaatc
    21661 atctagataa tccaagatct tttctttgtc ttcaaaattg ttcggataaa tcatgatact
    21721 tgaaggcaag ctgtctccac ctaaataact aagcaatgtt tgtttggcat tttcgtcaac
    21781 tttttcagta gttaagacat ttacaccact gtctctttga gctttgacga tttctgattc
    21841 tttgttttcg ttgacgattt gagtcgttaa ttgatcgcta taagcgattc ctggagataa
    21901 gagattcatt gtagaagaag atttcacgcg taggatcccc gagatcttca attcatcaga
    21961 agcattctga tacatcgcat cataatctgt attagggata aaattcccag ttggaagttt
    22021 tgtatagtaa gtattgttgt ttactaattt gaatgttgta ccaacgatat catcaaaatc
    22081 aagtttttcg tcttctttca catcaaatcc aagattcttc aatgcgttga tattagtatt
    22141 attattgccg tctacgatca gcacgacatc tgttgctgag gctggataac ttccggcaag
    22201 taaagaataa ttgtctttta aaaagttttc tttgctagtg tcaagctgtg tagggaaact
    22261 tgacactccc acacctgtca ttgctgacat cgtactagat aaagaaagag attccgtatc
    22321 aggattttga ttcgagaagc tgacaggctg tactttgcca tttacatcac gtaaaagatt
    22381 gattcctgta gtacgggtaa atccgatatt attacttaag ttaggatcga tgtctgttac
    22441 gtaatcgacg tatgtttgat caatcttatt cgtgtgctgt gctttatctt cttcgctgac
    22501 ttttgcagta atcgttttgc tgtctggata atccgctttg cttgcaccca atccggcatc
    22561 gtcacgagtc tggttagtcg ttaccttaga aatagtaatc ggaaatttcg ccatcgtttc
    22621 agcttgcgtg ttatcgattt gtttttggaa tccagaagaa agggacagaa cgatcgcgat
    22681 tccgatgatc ccaatactgg aagcaaagga ggtcaagaaa gtacgtcctt tttttgttcg
    22741 gatattgttg aaggaaagct ttaacgctgt ccaaaaactc atctttgttc tgcgtaagtt
    22801 aaattgatca tcttttggtc gttcgatgtg aggatgggaa tctgttaaga tcttaccatc
    22861 agaaaattca atgattcgat ctgcgtattg atgggccagt tcaggattat gtgtgaccat
    22921 gatcactaat ttttctttcg ataactcttg gatcaattcc atgatctgga tacttgtttc
    22981 tgtatctaac gctccggttg gttcatcgca caaaaggata tctgggtcat tggctagtgc
    23041 tcgagcgatg gccactcgct gcatttgtcc accagataac tgatttggct ttttatgcat
    23101 atgatttgtc aagccgactc ggtgcaatgc atcttctgcc cgttttcgtt tttcgtcttt
    23161 agaaacacca cttaatgtca tcccaagttc aacgttttca ataatgccta gatgaccaat
    23221 caagttgtaa ctttgaaaaa tgaaaccgat agagttgttt cggtaggcat cccagtcact
    23281 gtccttaaag tctttagtag actttccgtt gattaccata tcaccggaat cataattatc
    23341 tagtccacca atcacattca gcatagtcgt tttaccagaa ccactaggac ctaaaatcgc
    23401 tacgaattct ttttgtctga aagcgacaga cacaccatcc aatgctttgg tggtggtttc
    23461 gccgacttta taatattttt ttatatcttt taattgaagc ataatcaccc ttctcccgta
    23521 ttgaaatatt cggtatacta ataagtatat cagaacaatg atcgtagtat acgaaataaa
    23581 tgttaactga agatgaacta caagaaaaaa tctagaaatt tttcaaaaaa cagagggaga
    23641 tcaatgaatc gtattttagt agtagaagat gatccgcaat tacagttgct ttaccgttct
    23701 gtgttggaac gagcgggctt ttcagtcatc accgcattga atggagagga tgcgctgaaa
    23761 aaactagaag cgacacgtat agaattaatc attacagata tcatgatgcc gaaaatggat
    23821 ggctatgact tgctagactc attacgtagc accagatttg acacacctgt tctaatgatt
    23881 acggcaaagg ccgattttga ggataagaaa cgcgggttca aattaggagc agatgattat
    23941 atgacaaaac ccatcgatgt caatgagatg atcctacgtg tggaagcatt gcttcgacgt
    24001 tcaaagatca attacagcag tagcttgata atcggacaaa caaaattaga gctagatgct
    24061 tatacagtgt tacaagatca gcattcgttg attctgcctc aaaaagaatt tctgcttctg
    24121 tataaattac tgtcttatcc caacaagatc ttcactcgcc aacagttgat ggatgatatt
    24181 tggggattag atacgaatac agaagaacga acgatcgatg tacatatcaa acgtttaagg
    24241 acccgttttg aaaagacgga agattttcaa atcatcacag tacggggact aggctataag
    24301 gcggttattt aaagatgaaa aaaaagctat tttcacaatt atggatctat tttgctagta
    24361 ttgtcttcat ttcgatctta gtgacactgc tgtgttttct cggttttatt ttcttattgt
    24421 ctcgacattc tatctcgcct gctgaatcgg ctggaaaacc aacgttatac ccgttattcg
    24481 tctttgcggg gttcagtatg gtcgtgggta caggaatatc gatttttgtt gggcggcgta
    24541 tcctgcatcc aatcagtgcg ctagggacaa atatgagtct ggtagcaacg ggagattttt
    24601 cgattcgaat ggatgaacag cagaaagtag cagaagtaca gcaactgtat aaagatttca
    24661 acgttatggt ccaagaactc aatagtatcg agacactgag gaatgacttt gtgtccagtg
    24721 tatcccatga attcaaaaca ccactagcaa caatccaagg atatgttcaa ttgttacaag
    24781 ccccaaatct ctctgacgaa gaacgccaaa tatttttata tcggattatc gaaagtatca
    24841 cgcagctttc acaattaaca gagaacatct taaaattgaa caaactggaa aaccagcgta
    24901 tccaactgga aaagaaagag tatcgattag atgaacaaat cagagaagta atcgtttttt
    24961 ttcagcctaa atgggagaaa gaacagctag aattggatat cgaacttgct gcagtaaatt
    25021 acacaggcaa tgaagagttt ttgtaccaag tgtggctgaa tatcatggat aacgccataa
    25081 agtacaatca aatcaatgga cagattcata tcaaactatt tgagacagcc acagagattg
    25141 tactggaagt gaccgattca ggtgtaggaa tgaatgaaga gaccagagat cgaatgtttg
    25201 aaaaatttta tcaaggagat acaagtcgtc aaatttctgg gaatgggcta ggactttcac
    25261 tggttaaaaa aattctagaa ctgcatgatg gacgtattga ttatagcagt atcgaaggag
    25321 taggaacgac agcaatgata cggttaagta aacaatagga aaaagaataa aacccctaaa
    25381 aattctattg aaggttttta ggggttttta gtcgtttttt tgtgattagg gaaatgttct
    25441 tacggttctt ttggcataaa aatcgctgct ttcttattga actacgagaa atgttagagg
    25501 tgtcattgaa acaaggacat gatgcaagaa agagagcgaa agtcatgcgt cgctctcttt
    25561 ttttgggtgg atagatggac agaaaatgta gggcttagtt ttcttcttga tttaaatact
    25621 cccaaaatgt atctgaagcc aaagaatgct caccgacttt acgagtaaat gcaagataac
    25681 tctctgttgt tggagaaata gggagaatca cagtattttc ttttaataga ttcttgactg
    25741 ttttttccat aacaatagca atccccatgt catttgcaat catattgatg atcaagtcga
    25801 ttcttgctcc tttataagag atttttggtt caaatccagc ttcgcgacac agatctagta
    25861 caggttgata caagttcgta gtggggccaa ggagcaaaaa gttctccttt ttcagttcgc
    25921 ttagatccaa tttcttttta ctagctaaag gatgattttt aggtagaact gctacaaaat
    25981 cgtctgtttc agtaggcaac cactcaatcg aacttggtgg ggcctggaaa cttctagcaa
    26041 agtggatcgt gtcactatgt tcattggcgt cattgtaagt taattcgaca ctttccactt
    26101 ctttcaactg gactgtaaat tcaggatggc gtttcaaaaa ctcggtgatt ttggaaaaac
    26161 ctttataatt gatcattgtc ggtatcgtta agatatttaa tgaaagattg ttgtctttcg
    26221 tataggtttc aagcacttct tgcatagcat ggtattgttc tacgacattt tttacatatg
    26281 ggagcaataa gcttccagct tcggtcaatt cgatttttct atgggcgcgt tcgaataatt
    26341 tgacgtctaa ttctgtttct aacgaaagaa tctgtttaga aatatttcct tgggtggtga
    26401 attgtcgatc agcagttttg gtgaaattca ttgtttcggc taaatcaaga aaaacttcga
    26461 atttatggat atccatcata ttcctcctac ttattccaat aaagaatact ttaacacaaa
    26521 aaatgaattg gtgtaatctt ctctgtctgt tacaatcttt cttgcagcga aagcaataat
    26581 tacagaaatt tttctgattt ttttcgcttc gtagaaaaag agaggagttt tttgatgcca
    26641 actggagttt tgatcaatgt aggttccgtt ttgtttggcg gactaattgg cggattagta
    26701 ggaaataaat taagtgaaca ttttaaagca cagctgacga tggtttttgg tgtgtgttcg
    26761 atggggatgg gaatctattc cattgcgccg atgaaaaata tgccggctgt tatttttgct
    26821 ttagtgatag gaacagcgat tggtttgatt gttcatttag gtaatgggat aaataagggg
    26881 gctgcgctga tgcaagtacc tatttctaaa atcttcccta gtgaaaagct tggtatgacc
    26941 cacgatgaat ttatcagcac attagtgacg gtcattgttc tattttgtgc aagtggtacg
    27001 gggatctatg gaagtttaga ttctgggatg acaggagaca gtacgatttt gatttctaaa
    27061 tctgtccttg attttttcac agcagctatt tttgcgtgta atttagggta tgtcgtttct
    27121 gtgatagcaa ttccccaatt cattattttt tatattttgt tcttattggc aaaatttatt
    27181 tatccattga ctacaccaga catgatctta gattttaaag cttgcggcgg attcttgatg
    27241 gtcgctaccg gttttagaat gatcaacgtg aaaatgttcc cagtagctga catgatacct
    27301 gcaatgatcg ttatcatgcc actgagctgg atgtggacaa attggattat gccactgtta
    27361 taataaaaat caaagagaag ccattttttg ctgactaaaa aggatcaaat cttttagtca
    27421 gtttttttgt atttgacaaa ctgattttga aactatatta tatgttgtat aatatagatt
    27481 gaaataaaat attacacttg agaatagaag gtgacgtaat gcacatccag attccaactg
    27541 ttcttcttga tggtactgtg ctagcagcat taagcaagga agatatgtat gggtatgcct
    27601 taacaaaaat cgtacaagaa agtctctctg tcagtgaatc tacactatat ccggttttga
    27661 gaagattgaa aaaagaagga tatcttgaga cctatgatga accttttgaa ggcagaaacc
    27721 gtcgatatta ctcgttgaca ccaatgggaa gtcgtcattt agaagaaatt attgatgaat
    27781 ggattgtttt tcgagattct ttagaccggt tattggagga gagcacaaat gaatgaatat
    27841 ttgaatgaag ttgtggatca tcttcaagaa attcctgaag acgatcgttt tgatttgatc
    27901 cagtattatg aggaatattt tttagattct ggaaaaacat tagatcagat cattgatgaa
    27961 tatggtacac caaagcagtt tgccttaaaa ttaaaaatca gttacttttc agaagaagaa
    28021 caagcagaaa acgagttagt gaaaaatcca aaaagacaaa ttcgcctgat ttggttaatc
    28081 attatcggtt tatttgcttc tcctgtcctc attccactag cactggggtt tatattggtt
    28141 attgggggac tattggttgc tttactagcg ctcattttta gtatctatgt tttatgtatc
    28201 agtatcttag gtgttggcct attcatgatc atctcaggaa tcagtgtgtt tggacaatcg
    28261 attctgagtg gattattctt tatcggtgtc ggtctgttcg ccacaggaag cgcagtattt
    28321 atttcgccac tgttgctgaa aggaacaaaa gggctttttt atgtcatgat gaactttgtt
    28381 aaatggatgg gacgacgttt tattacaaaa agagatctgc attcagcggg cgtttgaagg
    28441 aggacacata aattgagatt gaaacatttt ttgattaccg gtattggtct gatgcttact
    28501 ggtggcctcc tagcaggtgc aacttataca ttaggcgctc aaaagagtct gacttgggat
    28561 aatggtcctc ggttgatcga tatggttact gagacaaaaa aaatcaagga tgcaaacaaa
    28621 atcgtcatcc atgcagaaaa tcaaacagtt tcggtcatac gcggattgga ttttgagata
    28681 aaaacgactt atgataagac agaaaagcca gaaataaagg agaaaaatgg cgtgttgact
    28741 gtgaatgcag tcaaaaaatc aacaggtgca atcattagtt tagaagacac aagtgcacaa
    28801 atcactattg ttgtccctgc agatcatgaa ttatctgaat tgacaatcga agggaataat
    28861 agtgcaattg atatcaacga agtatcaatc aaaaaagtga atactaagac acatgatggc
    28921 tgggtatcgt tcaatgaatt tacaggagga acaataactg cgaaagatga attaggctca
    28981 cttcgattag atgaggccaa tgtggataac ttgatcgtca caggtgaaaa cagcaacttc
    29041 catctagaag acagtttgat cagcaaagaa ggaaaagtca tcctaaacgg tggcaattta
    29101 tctttgcatg agatggaaaa cagtggctat actatccaaa cgtctggtga atcttatatt
    29161 gaaaaagact accagccaat aacaaatact ttgttagaac aaggacagaa aaagctaaag
    29221 atcactgcta aagacacatc gatcaatatt acgacggatc aagaggacaa tgaaacagat
    29281 tgatcctgta ctcaactgga tattttaaga aaaaacagca aagatccttc caatctttgc
    29341 tgtttttcat cgcttaacgc acataactat agtaaaataa gaaagaaaat atctaaaaag
    29401 aaaacagggt gaaagagtat atgaaggtag aagaaggtca aagagaaaag ttaaaaacag
    29461 aaatggaaag attacatact tatatcactc aactaagcca aaccttctat gatccagaca
    29521 aagaaaaagt gatggttaat tacccgaaca attcagaagg tcgccaatta gagcaagtgt
    29581 accatgaagt tttcaaacat ttattgacgg tgaagaaaga gctggattat tattctttac
    29641 cgattatcga tacaggaata ttgaagtatg atgggaagaa ggaacgattt atctttaaat
    29701 ctgttcggga agatttgcct ctttcagcag ggatggatct agaggtcttg gtagaagact
    29761 attttacaga agaaaagcac tgggttcgaa caaaattgga ttatttaccg caggcagccg
    29821 gagggacaca ggcaagcggt tggtacatca ctgaagacaa agagttagaa ctagaaggtg
    29881 taatggcgcg tattcgaaaa aaaaattaaa aaagtaacat tagatattta ttgaaggata
    29941 aaggagcgca ggaagtagca tgagagaaaa aagagcattt tatatcaatg gttatgcagg
    30001 gattgtcgga ttggtcgtat tagctttagt tggattgttt ttcttctatt taggtatgtg
    30061 gcaagcgaaa gtttgggcac tttttttaag tatttttctt tggttgatca cgttattgtt
    30121 gttaagttcg gcaactgtag tcagtcctaa tcaagcaaaa gtaattttgt tttttggaca
    30181 atatttaggt acgattcgag aaaatggctt tttccttacg atacctttgg cacaaaaaat
    30241 gactgtttcg ttgaaagttc ggaatttcaa tagttctgtt ttaaaagtca atgatttgga
    30301 tggaaacccc atcgaaatct cagcagtagt agtctttaaa gtcgttgata cagcaaaggc
    30361 attattcgat gtagcgtatt atcaagattt tgtagaaatc caaagtgaga cagccattcg
    30421 gcatatcgct agccaatacc cgtatgatac attcaatgag aatgatttga cgcttcgagg
    30481 aaacacgacg gcagtgtctg acgagttgca aaaagagctg caagagcgtt tagcagtagc
    30541 aggtgtcgaa gtaatcgaaa cacgcttgaa ccatttagcc tatgcaacag aaatcgctag
    30601 tgccatgctc caacgtcaac aagcaaaagc cattttgtct gctcgacaaa ccattgttga
    30661 aggagctgtc acaatcactc aaatggcctt agagcaaatc gaagatggac aagaaatcaa
    30721 tttcaccgat gatcggaaag tccaattgat caataattta cttgtctcaa tcattacaga
    30781 caaaggaacg caacctgtta tcaatacagg cgacatgaaa gaccgtaagt gacatacttt
    30841 ttgtaagata ggaagaagga gtttaggacg gattattgtc ttgaactctt tttttagttg
    30901 ttttaaagaa aatgacaaaa ttgaaagaag aatgatagtt gattcgaagg cagaattgtt
    30961 cgcttttttc tataatcagt atataaagta agagagaagg ggaaaaagtg aaaaaacttc
    31021 tgatcggtct acttgttttc atcgttgtgg tacttgcagg ggtgaaaatc gcagattacg
    31081 tagttatggg tggagaagaa tactatgtac aaattacgac agatggtaca aaaacagttt
    31141 caaaatcaga ctctggacaa gaaacagtca actatagata tatcctgcct ggctatacga
    31201 aagagggtaa ggaaaaagaa ctggacttca agggacaatt gactcgaccg cttcgtaaag
    31261 gagcatattt aaaaatcacc tggaacaaaa ataaaggtgt cacttcgtat gaagaagtcg
    31321 aaaaaaaaga cgtgccgaaa acagcactgg aaaaactaat taaagggtag ggaaagaaat
    31381 gaacaaaata gtcgaagtac agaatgtaac aaaagtatac ggaaaaaaca atgagaaaaa
    31441 gacgcaagca ttaagcggca tcagcttcga tgtagaaaaa ggcgaattta ttgggatcat
    31501 gggcgcttct ggttctggta agtcgacact tttgaatatt ttatccacat tagataaacc
    31561 aacagatggg catatccgaa tcaatcaaaa agatgtgacc acattaaaag gaaatcaact
    31621 agcagatttt cgtgcgaatg agattggctt tattttccaa gatttcaatt tattagaaag
    31681 cttgacagca caagaaaata tcgctgttcc tctttcttta caaggcgtca gaccaaaaga
    31741 aatcaagcaa cgtgtccaaa aaattgcaga acgtttatcc atctctcata ttttggaaag
    31801 ctatccttct gagatttccg gtggacaaaa acaacgggtc gccgccgcac gtgcattgat
    31861 cacacaacca acgattctat taggggatga gcctacaggt gcgctggatt ccaaaagtgc
    31921 ccgggatctt ttagatacga tggacgaatt gaatacaaaa gatcatgtat cgattcttct
    31981 tgtgacgcat gatccgtttt ctgcaagtta ttgccaacgt attttattca tcaaggatgt
    32041 cggtatccat caagaagtga aacgtggaga tcaaagccgt gattccttct atcgagaaat
    32101 actatctatt cttggaaact tggaacagta gggggagatt gactatgctt tggaaattat
    32161 cacttactgg gatcaagagc cgtttaagag actacttggt gttattctca ggattagtta
    32221 tggcatctgc tattttttac atgtttgaat caatggcaag caacgaagct tttttgaaaa
    32281 gtaatacgat cgttgcggct acagttatca ttttccgatt cggttctgtt ttactaggga
    32341 tcatcacatt cgtttatatt ttatatgcaa attccttttt gatgacgatg cggcaaaaag
    32401 actatgcaat gtttatgatg ctgggagcaa aaggcagaaa aatcgcacag atgatttttt
    32461 ctgaaacatt cttagtaggg atcgcagcta ctttagtggg ttcagctgtc ggtgtaggat
    32521 tgacaagtat cgtccaccga ttattagtgg atcagttaga tctcaatatt tcgcatttta
    32581 caccttttag tattcacggg ttgttgatca ctgttttgtt ttttgctgtt ttgtttttat
    32641 tagctgctgt tgtcaatgct ttttcgattg tcaaaaaacc gattttagca ttgattcgtg
    32701 cggatcagac accaacaaga atcaagcagc ataaattctt gtttttcctt gaagtagtac
    32761 tgggaattgc ttttctaggc tgcggttact atatgatgaa acacgtgggg acctatcaag
    32821 tctttggtat aggggtagcc ttagttacga tcgtattagg aacttatttc attttccatt
    32881 ctgtgatcat tttcttttta tcattactaa agaaatcaga acagatttca ttgaaaaaat
    32941 tgaacaattt cacattgtca cagttaaact ttcgtattcg tgaatataca caaatgctct
    33001 caatggtcgc tatgctgttt gccttggcac tgggcgcttt gacggtagga ttaggtttta
    33061 aaaatgaaat tgcagacttg acagaaaaca attctggata tgatctagtg atgaataatc
    33121 ctcgggcaga agatcagcaa aaaatcaaag aactttcacc aacactttct gcttcttata
    33181 ctcaaaaaga agatgcggga acgatttact ataatgcaag tgaatttgcg gctcaaccat
    33241 tgatcatgat aaaacatgaa gatggaacac cgccgaagat cacgaaagaa aaagttcctg
    33301 ctaaaaaaat gaatgaactg actgtacaag aggaacttcg aagctatgaa ttgccggaac
    33361 agcaagaaaa agaaatcaag gtagtcagtc aatcagaatt tgatcaactg aacttgccac
    33421 aatccacttt acagctagtt caggtaaaag acttccaagc aaacctgaaa ccaatcgaaa
    33481 cacttgttgc tcaaaataaa atgaacaatc catctttgca aaaagtagag tacagcaacc
    33541 agaaatatga aatgtatgaa gtgtataatg gtctattctc tggttttgaa tttatgggat
    33601 tcttcctagg tttagctttc ttgaccatgc ttgctagctg tttgatgttc aagatcttgt
    33661 caggaagcaa gagtgacatt gctcgttatg aaatgcttga aaagatagga acacgacgcg
    33721 ggttgcttaa acaatcgatc cgtagagaag taggcgtgtt attcttagca ccaggaatct
    33781 taggtgtagt ccatgtctta ttcggattac aaatgtttgg tctattcatg caaaatccat
    33841 acaaagatat ttgggttccg tttacgatat tcttcgtatt gtatttcatc tactatgtac
    33901 tgacgacatg gttatatact ggaatcgtat tgaaaaagag attgtgaaaa agctgtcctg
    33961 catcaagtaa taagaacagc caagcaaaaa tagctcctca tatttttcgt ttggttgggc
    34021 ttattaccgc agatgcaagc ttttgaacac cgtttgttcg gaaaaagagg ttgtgacaat
    34081 atttttgtca caaccttttt ttcacggtct tttattagaa tatagtaaat tttttctcgt
    34141 tactcacgga ataagctgtt aagaaaacgg tttccatgta tactggaatc agtgcaaatg
    34201 aaagaaaaga gggtgcaagg aacatggaaa tcaatgaaca aaattttggc gaaaactatc
    34261 acttgattac gatcacgaat caaaatggga catctttgtc tgtcagtgat cttggggcac
    34321 gggttgttca ttttcaaaca ataatcgata atgaaaaacg agagttgatc ttaggttttg
    34381 atgaagcaaa agaatatcta gaaaaagacc cttatatcgg tgcttcaatc ggacgtatgg
    34441 ctggacggat tacgaatggc acattcaaat tagatgatga aacgttcgaa gtaaaaacag
    34501 acccggacac tggtcattgt ttacatggtg gtgcacctag ctttgaagcc aagcagtggc
    34561 attatacagt catcaatggt gaaaacgaag catcggtcat tttctatcag actagcccag
    34621 atatggaaaa cggatttcct ggaactttag atgtagaagt acggtatacg ctgacgaacg
    34681 atgatacatg gcgagtgacg gtccaaggga tcagtgaccg caaaacagta ttcaatccta
    34741 caaatcatgt ctatttcaat ttaaccggtg atccagcaca gtcaatcgac gaacatatac
    34801 tttggttgaa tagccgatta tttgctacgc tcaataaaga tacaacgcct acaggagaag
    34861 tagtttcagt aagtggcaca gcatttgact tccaacgtcc taaaaaatta ggtgaggtct
    34921 ttgccagtga ttttcgccaa tcaatgatag tgggcggcat cgatcatcca tttttgttga
    34981 aaaaacaatc tggtttacta aatgctgcgg aattgacaag cccagatgaa aaagtatcga
    35041 tccaagtaaa aacagatgct tcaggaatcg tgatttttac agcaaatttt ggagaagatg
    35101 gaccggaaat gcgcgggaat aaattagtaa atcatggcgg tatcacattt gagactcaag
    35161 aactgccagg agcggagcga cttgaaagtt ttggagatgt cactctggaa cctaaccaag
    35221 tacgcgagtt tgtaacggaa tacaaaataa atataaagaa atagaataaa ttaaaaaggg
    35281 agtgtgacag tgtcacatcc cctttttaat ttattctatt tctttaaagg cagatcaagc
    35341 cagaatgatt tgtctaaact taacaaggtt agtacaggaa cagttgcagc tttaactgta
    35401 cgagtagttg tttctggtat ctgtcttgcc gtaaaattgt tccacaaatc ccagattgaa
    35461 gcgacacgga agatggaaga agcgttgttg acgcgtttta tcgttgaaaa ttctaatcct
    35521 gtgattggca cgccatctgt tgtccatttg attggcacat cgaatcgctt agacatattg
    35581 aggatttcag cgtttgcttc ttcagttgtt gctgcttctt ttttcggttc atcatatttt
    35641 gtcaggtcgt acgcttcgat gatcgcgttc aatttttgtg aatattggcg atctgtagca
    35701 taacgtcctt ccaaatattt ggttgcttct ttgtagtctt ttgtttccga cttccaagtt
    35761 ggtttgtaga aatcaggatt gccagtcagt ccatttttga tcaagtcggc atagtcttcc
    35821 aaactttcct tgtaagaagg atatttacgg aattgtgcgc ggatttggta gctttgtccg
    35881 gaactatctt gttcaagtgt attgaaggtc acgctgcttc ctttatatga tcctttgata
    35941 ccaaaaaggt tatgatttgg ttcgcttgat aataagctgt ttccagatcc actttcaagg
    36001 atagcttgag ccatcatgac tgatgcgtag attccttctt tttgcccgat ttcctgtgca
    36061 tcatctgcaa tctttttcaa gaactgatca gttgtttggt tttgactgaa atggaacgag
    36121 ccatctaatc catctaaaat agatgagccg cctgtcagcg aacctgaagc agcaggtaaa
    36181 cctgctaagt ttactcggac agcaaaatca ggcgtgaaat ctgaaataga agttactcga
    36241 actttatctc ctggttgagg cgcatggata aattgtccgt tcccaatata gatacctaca
    36301 tggtgagtaa caccggaaga aggtccccag aagacgaggt cgccaggttg ggcctgactt
    36361 actggaatct gcgtacctgc atattgctgt tcgcctgtcc agccaccgat ataatgaccg
    36421 gttgctttca tatatacgta ataagtcaaa ccagaacagt caaaagtatt tggacctttt
    36481 gctccccata catatggttt tccaagatgc ttgaatgctt ctcgaacgat tgcttgttgg
    36541 acattcgttg tatttgtagg caatacagca tcaattggtg attttacaac aaaatcagaa
    36601 gaagcagaag atggattctc tataccgctt tgatttgctg tatttggacg agatgaagca
    36661 ttgttcgttc cgccattcga tgaaccattg tttggatggt ttggttctct ctgagtattt
    36721 ccattgttat tcgatccaga tgggtttgtt tgattcgaag aattcccgct gttcgaatta
    36781 ttgttattat tgtttgaatt atttgaacca ttattattgt tgttattacc cacatctggt
    36841 ttgtttggcg tttccggatc tggtgtacta gggacttctt cactaggatc agttggtttc
    36901 tctgttgaag tatccggtcc agctttaaat tctgcagtaa tagataaatt gttcgtacct
    36961 actgtaaagg tctttgcatc ataatttact tctttggcac cattaacagc gactgaagca
    37021 atttgttcac cttcagctgg cgtaatagtg taagttacct tcgtttccgc taataaacct
    37081 tctaccgttc cactttccgt aatatccaaa ggttgctgtt caggggaatc cgctgtcgaa
    37141 gcagtcagtt ggatcttacc aatcaaagaa ggagcaattg taagggaata tgtctcggct
    37201 ggcttttcat tttctgatgg atcagccgga tcaatttcag ttggctcggt cgatcctgtt
    37261 gttgagttag ttgttccagt tgggtcttct gtaaaagaat ccgttgtttc actagtagct
    37321 gagctggatg tagtcgtatc tgttgtgact gtttgatcag ctatttgatt atctacggca
    37381 aatacggtat tggtcgacaa aacagacgga gcaatcagcc aagtagcagc tagcaaacgt
    37441 atcgtttttt tatttttcat aataaactag tccttttcct ttcattgatt ttctgtctta
    37501 ttcagcattc attagacatt ttttggaaca gataatcgtg tcgaaaagct ttttcttttc
    37561 ataaatctgt ctgtgtattc taaataaaac aatttgtaaa taagcaacga gcattcattc
    37621 gtttttcttt gacgttcatt ttattgcata gaccaccttt gtttggggat aaacaagtgg
    37681 ggataaaaat ggttatgcat atgtaaatta taccaaagtt tggaaagtat cacaaaaatt
    37741 tataaaaata atgatcctaa aaagaaaaag actgaaatat gtgataaaca tctcatactt
    37801 cagtcttttt cattaactat tcataagaca ttaggtcttc atcaaacccg ctaacgagag
    37861 tagtgatcat tgctacgact agactaacag ccagtcctgc taagtaccag aaaaaagtac
    37921 cggaaatgaa tgcaggcaat gtggtcagac taccaaatac aaaagcgatc gctttcactt
    37981 gggcaaatcc cattacagct ccaccagcag ctgcaccaat gcaagcagca acaaatggtt
    38041 ttttcaatgg gatagcaatt ccgtaaacag ttggttcggt cactcccgca agtaaaatcg
    38101 aagcaaaagc agaaccggag atcgaacggg tttttgaacg tttacttcgc agaaatacac
    38161 ctaaagctgc accagccatt ccaaaattgg ctgctcctgt caacggaacg attcggtcga
    38221 acccataagc agagatatta ctgatcatga cagggtttac agcccattgg ataccaaata
    38281 ttaccaaaat attccaacca ccagcgatga ttgcgccact gatcggtccg ttaatatcca
    38341 tcatccaagt aattgcggca gctaaacctt cgccaaggta aacacccgca ggcccgaata
    38401 ccattaacgt caaaggaacc gtgataacta atgaaagtaa aggtacaaat actaattgca
    38461 tattttcgcg aatatatttt ttgaggaatt tttcaacaaa agaataaaag aaaataccta
    38521 aaatcgccgg catgacagta gacgtatact gcatcagtac tacaggaatc cccagaaaat
    38581 cagtagctgc tttgccactg tctgaaagta aagaggtcag attcggttca atcagtgctg
    38641 cagcaatgac agccgcaata tatccattca ctttaaacgt tttggcagca gaaaaagcaa
    38701 gaaagattgg cagaaaataa aagacggcat tcgacgcagc agacaaaata tgataagttc
    38761 cgcttgtttc actaagccag ccaatcgtag tcgctaaaac aaccagacct tttaatatac
    38821 cagaaccggc aaacagcgga atgatcggcg taaagatttc cgatatcaaa ccaaatagtt
    38881 tttcgatcgg atttagcttt tctttcggag ctgaatgatc gatttgacgc gtcaattcct
    38941 tgtaagcagg agccaatccc tgatccatga ttatttctgc atatctatcg agtaaaatga
    39001 aatacttaat ggaagggatt tgctgtttta atttttctgc attggctact ggctgttttt
    39061 tcaatttgat acgaagccga gtcgcacatg gaaggacaga atcaatattg tcttttcctc
    39121 ccaactgttc aatcagcata tgaatctgct cggacagtac catctgttca ggattcattg
    39181 tcgtttccat tagaagacca ctccactcac taatataata ttggcgtaag gtgtcatttc
    39241 gcctgtacga ataaatgcat gacagttatt caactctttt ttcatttctt catgtgggat
    39301 aaagctaatc ggtgtatctg gtaacaataa tttgattttt tccaatattt ccgggttcat
    39361 tgtctttatt tcttccgcca attcaatcga ttgtacctca agttcttcta aaacgttttc
    39421 taaaacagat aaaaaacttg gaagaccgtt tgagacagcc aaatctattt tttcagtacc
    39481 aaaggggaca ggcatgcctg catccccgat acttaattta tcaaagtgcc ccatttgtgc
    39541 gattactcgt gaaatatctg aattgattac ttttcctttt ttcatgtttg tttcttcctc
    39601 tcattgttaa ttttagactg ctgattgttc taaagcttcg cggtaaggga tagaaggttg
    39661 cgcaccataa gactgcacag caacagaaga ggctagattt ccataaagga tcgcttcttt
    39721 caagttacta aggtctggct gaagtgtgct gcttaatgcg ccaataaatg tatcacctgc
    39781 ggctgttgtg tcaacggcat ccactttaaa agcagggacg ataccgtgtt ctttttctgt
    39841 atgatagaaa gcgccggcac tgcctagtgt aatgattact gttccaatac ctaattcatg
    39901 gagtttttcg gctgctgcga ccaaactgtt atgatctgtc actctgattc ctgtaatgat
    39961 ttctgtttct gtttcatttg gtataatgat atctgtcttt tcaagtagtt cagtaggaat
    40021 cgtttcacga gctggtgcag gattcaggat cgttgttttt cctgcgtctt ttgcaataga
    40081 aaaagctagg atcgtcgcat ctaagctact ttcaaactga gcaataataa agtcactgtt
    40141 tgtaatcagt tgcttgtttt ttaggacttc ttgttcatgg aaagcattat ttgcccctgc
    40201 atggatcaag atactatttt ctcctgctgc gtctaccata atcattgctt gtccggttgt
    40261 ggcgccttgg atttcagcta tcccagatgt atcgatgttt tcttttgtca gtagatctaa
    40321 taactgttgt ccttcgctgt cagccccaac gcctccaata aaagatgtcc gtgcgcctga
    40381 acgtttggct gccactgctt ggttggcacc tttacctccg ccagcgtgaa aaatctcatg
    40441 cgcatgcatc gtttcgcctg gcttgggcat gttggttaaa cgaagggtag tgtccatatt
    40501 aatacttcca attactgtaa ccgtgttcat tttacaaacc tccaactatc tcatttattt
    40561 tgatgggaaa attatttttt aaaacgtttt agtaaaacgt tttaatagtt cgataaaaat
    40621 atagcacacc ttgaaagcta tttcaagggt gcagttgaaa atcttttttc taatcgtaca
    40681 ggtaatcttt tttcttccca ttctttttct gggaattgga tccgatctaa caataacttg
    40741 gcagatgttt gtcccaattc gaagattggt tgtgcgatcg ttgtcagttt cggcttgata
    40801 tactcgcaca tatctatatt atcatagcca ataatgctgt agtcttcagg gatgcttttt
    40861 ccagcttcct ctaatccgcg atataatcca aaagctaatt catcatttaa agcaaacaca
    40921 ccagttgcag cagaatcaag aagttctgct gtgatctggt agccaccttg ttttgagaac
    40981 tgtgtaggta agaggatcag ttggtcgtga gggatttggt aaacatcaag agcagattta
    41041 aacccctcaa tccgagcgtg gacattttcc ggtggatttt caggatagac aagggcgatc
    41101 gtttgatgtc ctagactaag caagtgcatg cccgctaggt aaccaccgcg aaaatcgtct
    41161 gttcgtacgg catcactaaa tccttcagat tttttctgat ccaaaacaat gaagggccgg
    41221 ccttgttttt tcaaattttc gttgattgca tctgtagaga cagcagaagt cgcgataatg
    41281 aatccatcca cacctcgacg tgtgagctcg gctagatatt cgatttcttt ttgatgatca
    41341 gaatcagcat tacataatat cgtgacgaaa ttctgcttat acaagatatc ctcaattcct
    41401 cgcatcagtg tactgaagaa tggattcgtg atatcaggaa cgagtacacc aattgttttc
    41461 gtttccttgg taatcatttg tctagcaaaa tagtcaggct gatagcctaa ttcttctttg
    41521 gcagctaaaa ctttctgaac agttttcgga ctgaatttct ccgtatttcc atttaatatc
    41581 aaggagacag ttgtgattga aacgccggaa agtttagcga cttctttgat tgtgatcttt
    41641 tttttcacca tgttcatact cctatctaaa tcctgtggtt attttcccat gaaatcaaaa
    41701 agaagtaaat gaaaatcacg tgtgccagta cttatttgct ttgttggata gcttcgatta
    41761 tttgtttcgt tgagcgtact cgtgcagttt tcggaaatat cttttttatg aacaattgat
    41821 gggattcttt gtcacgatca ctgcaagcat cttcagcaag cacgatatgg tacccgtgtt
    41881 gaaaggcatc taatgcagta gcatatacac cgtttgttgt cgctacaccg cttaaaatga
    41941 tagtttcgat tccacgccgg cgaagttgaa gatcaagaga agtaccaaaa aaagcacttg
    42001 gattgtatta tgtgatagtc aacatattat ctgtatcttt caaagaatcc ttcaaaagta
    42061 aatcagtgaa ttctggaggt acagaagcag tcattccacc catatcagtt tgttgactca
    42121 aataagggaa agtggagatg tctacgttca ctaaaacttt caaggcagcg gtacgtttca
    42181 atgttcttac taacaaatca ttgtttgtca gaatagatgc tgcagtgtgt ggagagagtg
    42241 taccgttatt tacgatctct ttttgcatgt cgatcgcgac aaaagcagtt tttgataaat
    42301 caaaaatcat attttccttt aaatcattca tgatgttcct tctttcaaat aacaatgttt
    42361 tttatgtaag taaatcataa atcgtttatt tgtcaactaa attaaaggat tcctttcttt
    42421 agtctagaga aattcagtgg taaaataaat aagtgtgaga aaacgcattc gatgcataga
    42481 tatagcgaga aatatcgtga aaatggagga atcagagtga agtatgtcga agtaaaaaat
    42541 gaatacaaac gatacaaaat gggagaaacg accattacag caaatgacga tatttctttt
    42601 tcaattgaaa aaggcgaatt agtgattata cttggtccaa gtggagcagg aaagtcaacg
    42661 attcttaata ttttaggtgg gatggataca cctgatgaag gtcagatcat tattgatgac
    42721 acagatattg cgcaattttc agataaacag cttactgctt accgacgaac agatgtaggg
    42781 ttcgttttcc aattctacaa tctcgttcct aatctaacag caaaagaaaa tgtggaattg
    42841 gcaacggaag tttctcctga tgcgcttgat cctgtagagg tattgaggca agtgggccta
    42901 gctcatcgat tgaataactt tccgtcccaa ttatcaggag gcgaacagca gagggtttca
    42961 attgctcgtg cactagctaa aaatcccaaa cttcttttat gtgatgaacc gacaggcgcg
    43021 ctggattttg aaacgggcaa gcaagtattg aaattattgc aaaacgctag cagacaacac
    43081 ggcaataccg ttttgatcat tacacataat tctgctttag cgcccatcgc tgatagagtc
    43141 attcacatta atgatgctaa agtaaggtct gttgaattaa atgatcatcc ttcatccatt
    43201 gatgagattg tatggtaggg ggattttatg aaaacaagaa catatcttaa agcaggtttt
    43261 cgcgaaattc ggcaatctaa aggacgattc attgcaatca tccttattgt catgctagga
    43321 acacttttat ttgtaggtgt caaaactgct ggacctgtca tgcaaaaaac gatggatcac
    43381 tatgttggta cagcaggact ttctgatttg caaatcgttt ctactggtgg cttgacacaa
    43441 aaagatattg ctcaagcaga aaagatccca gatgcaaaag tagaaacagg aaaacagatc
    43501 tattattcca atactggcaa aaacgaagtg attcaaatat tttcttataa caaaaataac
    43561 aagcagaaca agctacaatt aactgatgga aaactcccgg aaaaaccaaa ccagcttgta
    43621 ttggatgaaa aagcggaaga tgaagggtat aaaatcggtg atatttatca gattgattca
    43681 gatgaattga aagaaaaaga gtataccatc acgggctttg tccgttctcc attgttcatc
    43741 aacaatttgg agagaggata cgcgaacgtc ggaaatggta gcgtcgatta ttttgtctat
    43801 ttacctgaat ccgtttttaa aacagatatt tattctgtct tatatcttga ttttacaaac
    43861 gtaaagaatt tgaatacgta cagcaaagct tacaaagata aaatggagca aaatcaagaa
    43921 aaggcagaaa agtatttaca agatcgaccc gaagaacgct tagaagagct gaaaaaatcg
    43981 gccaacgaag aattagaacc ggcacagcaa aaaatagcgg atggcaaagc acaaacaacg
    44041 caagcaagaa cacggttaga agatgctaaa aaacagttag cgcaacagtc agcaactatc
    44101 cagcagcttc ctgaagcgca acgaacaatc gctgttcaaa cattatctca acaagaagct
    44161 caacttgctg aacaagaaag gcaattagtt gagaaagaac gtgaactggc ctctgcagag
    44221 gaagaattga atcgaaatgc agaaaaaatt gaaaatttaa ctaaaccatc atacctatac
    44281 aatgaacgtt cagaaaatgt cggttttcaa gaatttggtg atctgtctga tcgtatcgct
    44341 gcgattgcca atgtttttcc tgttttcttt ttctttattg ccgcattgat cacctttacg
    44401 acaatgacac gtatggtaga agaaaaccgc agggaaatcg ggacattaaa ggcgttaggt
    44461 tatacgaaag ttgaaatcgc aaaaaaatat gcaatctatg caagtctagc ctcagggata
    44521 ggtattgttt taggaacgat attaggaacg aatcttttgc caaggatcat ttttgagctg
    44581 tctaacgagc ggtatgatat tgggagtgcg cttatttttt atgactggcc gccgatttta
    44641 caagcagctg ccgcattctt cattgccgca tttggcgctg caatgatcgt tttattcaaa
    44701 gatttaagag aacggcctgc tgcattgctt caaccgaaag caccgaagcc agggaaacgc
    44761 atttttcttg aatacatcac tccgctgtgg tcacgcctta gctttaatca aaaaatcagt
    44821 taccgaaatc tcttccgata taagtcaaga atgcttatgg cgattatcgg aattgctgga
    44881 tgtgccgggc tgatggttgc aggcgtggga ttgaaagatt ctctaagttc tgtttcagcc
    44941 aaacaatttg gaccgatcat tgactatcaa gcaatcgtta cattggattc atccacggaa
    45001 gatagtcgtc aaaaagcagc ggatatgctg cagaagcaag aaaaagtaaa tgagatccta
    45061 gctattcata ctcaaaccgt agaattgaga aaaaaaggac atgcttcgca aagtctgaca
    45121 gtgatcgtac caagtaaaac agctgatttg gatcgtttta tccatttaac tgatcttgaa
    45181 gagaacccac gctccttgcc taaaacagga atcgctatta cgcaaaaagt agcagaacta
    45241 tttgacttat ctacaggaga tcatatttcg ctttatgatg aagatcaaaa agaatggaaa
    45301 gtcgaagtaa aagaaatcgt gaaaaactat ctaggtagct ttatttatat gtcccctgaa
    45361 tattatgctt cagttactga tcaatcgatg atggataatg catttttgct gcgtacagat
    45421 aagatgacaa caaaagaaga agaaatgctt tccgagaaac tgcttgccac agacgctgtg
    45481 acaaatacgt ctttcatgtc caaacaaatc gaaacgcaag aagaatcaat ggcgaactta
    45541 gactcggttg tcttaatctt tgtcgtatta tcaggactgc tggcttttat cgtcttgtat
    45601 aatctaacta atatcaatat ttccgagcgt gtacgcgaat tatctacaat aaaagtcctt
    45661 ggattttttg ataaagaagt cacgatgtac attgttcgag aaaatatcat ctttactctt
    45721 ttaggaatca tcggaggatt tggtgtagga tatatattga ctgacttcat cttgcaacaa
    45781 gcgtctatgg agaatgtcat ctttccatta gtgatcacat ggtgggctta tgtcttatca
    45841 gcaggattaa ctatcgtatt tacggttatt gtgatgattg ttacccattt caaattaaag
    45901 catatcgata tgattgatgc gttgaaatcg aatgaataaa aacagaggtt gtgaaatcag
    45961 cggtttgacc tgagtattag agaaacaaac ggcaaaatag tttttcatat tttgccgttt
    46021 tttgagctaa taccgaagga cagctgattg aacaccatga atcagaggtt gtaaaaaagc
    46081 cgtcctgcat cgagcaaaaa gaatactcaa ataaaattag ctcctcatgt tttacatttg
    46141 agaagactga ttgccgtaga taaaagcttt tgaacaccat ttatagaaga aacaagaata
    46201 ttttgctatt ttaaacaaga ggatagacaa atacatgtct gatctcttgt tttttattgg
    46261 gcaacagacc tcttagaatg tataaagata aattttacaa acgctttcgg ttcagttata
    46321 ataataaatg ataacttgta tatataagtt gtggtaggta ataaatcagt tgaatggagg
    46381 taaatatcca tgacatttaa agacaaagtc atttaccaaa tctatcccaa atcttacaaa
    46441 gacagtaatg gtgatggaat tggagattta aaaggaatca aagaaaaact cccgtatctg
    46501 aatgatctag gcatcgacat gatttggcta aaccctattt atccaagccc tcaaaaagac
    46561 aacggttatg atatctctga ttacacagcc atcgatccta tctttggtac gatggaggaa
    46621 ttcgaacagc taatagccga agcaaagaaa taccggatag aaatcatgct cgatatggtg
    46681 ctgaatcacg tttcgacaga acatgaatgg ttcaaaaaag cactagcagg ggaccagcat
    46741 tatcaggact tcttcatttt aagagaagag ccaacggatt gggtatcaaa atttggtgga
    46801 agtgcttggt cgccatttgg agatacgggg aaatattatt tgcacttata tgatcggaca
    46861 caagctgact tgaactggcg gaaccaagca gtaagagagg aactattcaa agttgttcgt
    46921 ttttggatgg aaaaaggggt aaaaggattc cgtttcgatg ttatcaatgt tatcggtaaa
    46981 gacgaacaat tgaaaaataa taaagaaaat gatggcaaac ctgaatatac ggacaaacca
    47041 atcacccata cgtatctaaa agaattgaac caagcaacgt ttgggacgga ttccactatc
    47101 atcacagtcg gtgaaatgag ctctacgacg atcgaaaatt gtatcttgta tacagaacct
    47161 agtagagaag aattatcaat ggtctttaat ttccatcatt tgaaagtcga ttatgaaaat
    47221 gggaataaat ggtctactcc tccatttgat tttgcagaat taaagcgact atttcataca
    47281 tggggagaaa agatgagtga gggtaacggc tggaatgctc tattttttaa taatcatgat
    47341 cagccacgtg cgctcaatcg atttgtagct atcgatcagt atcgaaaaca aggagcagag
    47401 atgctagcaa ccatgatcca tttgaaccgt gggacgccct atatttatat gggagaagaa
    47461 atcggcatga tcgacccaga ctattcatcg atttccgact atattgacgt agaaagtttg
    47521 aatgcttata agttactcct tgaacaagga ttcacagaaa aagaagcgtt tcgtcggatc
    47581 aaagcaaaat ctcgcgataa ctctcgaaca cccatgcaat ggtcagacac agaacaagca
    47641 ggatttacta cagggaaacc ttggttaaaa gttggaggca agctagaaga gattaatgta
    47701 gaaaaagaac tatcttctga agattctatt ttgtcctatt acaagaaatt gattcggctg
    47761 agaaaaacat atccaatcgt tgcacaagga gattatcatg cttatgaagc aaatcatccg
    47821 gaagtttatg gctttcttcg ccaatttgaa ggacagcagc tgcttgtatt aacaaatttc
    47881 tatgataaag atacaaggat cgccatccca gaaggattca cagaagccaa tattcttatc
    47941 gataattatt ccgatactgt tcttcatcaa agtctggagc taaaaccata ccaggcaatt
    48001 gctttgctga aaaattaaaa caaaaacaca aaacgtcgtt cctacgagct attctcgtaa
    48061 gatcgacgtt ttgtgtttta tttaaaataa tcttcttcag aaaatgaaga gttttcatac
    48121 tctcctttat cgccataaaa tttattgtag cgttgtttga atttacggaa aataaaagta
    48181 acgagtaatt tgactaacgt atagatagga ataccaaaaa taacgcccac aattcccatt
    48241 agatcaccca tgacaagtaa aacgattaag atcgtaatcg gatggatctg taaacgatca
    48301 cccattactc gaggaatcac caagtcaccg tggagaagtt gtacggcaaa ccagacaatg
    48361 acaaatttga cagccataaa agtagagtct tggaatgcaa tgatcaaacc tggaaagaag
    48421 gcgataaacg gcccaatata tggaatgata cataacaacc cagcagaaat agctaaaaca
    48481 ctagcaaatt ctaatccgat cagtaagaaa caaacccagt agactgctcc aaggataaat
    48541 gaagcaatga tttgcccctt tagaaatgaa cccaattgtt gattagcaat ctttgtcaaa
    48601 tttttaaaat ctgtacgaaa tgctggtggg acaatcgcta aaactgactg ataaaatttt
    48661 tgaggatctc tcaaaaggaa aaaagcgaca attggaccag taaataaagt aataaaaata
    48721 gctgtaacag ttgaaaaaat gttgctgatc cgttgtgcgc cactttccca ataatttcca
    48781 ataaatccag ctatatcaga cgtcacgtcg tcaatagaag cgaaaaattg attaaatgaa
    48841 tcggcaaaag gtgttttctc caaaaattgt cggagactct cttgaaaatc ttgaaacaaa
    48901 tctggcaaac tactgatcaa tgtctttgcc tgttcttcaa gaaaaggaat aagccatatg
    48961 cttccaagcc ccaataaaaa taaaataatc acataaacga taatgactga caagttcctc
    49021 gaaaggtgct tgctcaaacg tttgatgact ggttccaaaa gataatacaa gatcagtgca
    49081 aacaataatg ggggcaagat agtagcgata atgattccga caggcttgaa tataaacgag
    49141 atctgatgga aaaaataaat gatcagcgca agtaagataa ctattcctaa aacatagtaa
    49201 gaggtttttc ctcctaaaaa gcggatcaaa cgattcgttg tatctgtttc ttttttcact
    49261 ctttttcctc ctttatctat aaattcatta tagacaaaag cgggtataaa aggaaaatga
    49321 tagaggttga ttgcaaaaat atcgtcaatc attcatataa actttctcat caattaaaat
    49381 aagaaaacgc ttgacagttc ttttaaccat atgataaatt aaatcctgtt gctaaaacgt
    49441 aactattacg gtttgcgaaa agcgaatcgg aacattcaaa tgaaagaaga tgagaagagg
    49501 agtgtcataa atgaaacgat tgataggtat attggccatg ctggttttag caggtatgtt
    49561 aactgcttgc ggggcaagtg ggaaagccga ggacagtaaa gaaaaattga gcgtgatgac
    49621 gaccttttat cccatgtatg attttacaaa agcaatcgtc ggggatgaag gagaagtaga
    49681 gttattgatt ccagcaggga ctgattccca cgattatgaa ccttctgcta aagacatggc
    49741 aaagatccaa gatacagata tctttgttta taatgatgaa aacatggaaa catgggtccc
    49801 cgcaatccaa aaaacattac aagaaggcaa tgtccatacg ataaaagcga cagaaggtat
    49861 gcttttactt ccgggaagtg aagaaggaca tgatcatgac catgaacacg gggaagaagg
    49921 ccatacacat gagctagacc cacatgtatg gctagctcca agtttagcaa tcaagcaggt
    49981 agcaaacata cgtgatcagc tgattgaagc ttacccagaa aaacaggaag tatggacaaa
    50041 aaatgcagca gcctataccg agaaacttca agcactccat cagctttatc aagaaacctt
    50101 taagcaagca aaacaaagat cttttgtcac gcaacatgct gcattcaact atctagcgtt
    50161 agaatatgga ttgaatcaag tatcgattgc tggtctttct tctagtgaag aaccgtctgc
    50221 ggcgagaatt gctgaactga aacactttgt taaagaacat ggaatcaact atatttattt
    50281 tgaagaaaac gccaaagata gtattgccag aacgttagca aacgaagcag gtgtatcttt
    50341 agaagtattg aatcctttgg aaggattgac gaatgaacaa atagaaaatg gagaaaacta
    50401 tctttctatc atggaagcaa acctcgaagc gttgaaaaaa acgacagaga cagaaaaccc
    50461 gctagaagat actttgaccc ccaaaaaaga aaagagcgtc tacaacggtt attttgaaga
    50521 cgaggatgta caagatcgct ctctttcaga ttgggcagga acttggcaaa cagtagatac
    50581 gtatgtagaa gatgggactt ttgatcctgt ttttgaatat aaagaaaaat tgaatcagga
    50641 taagacagca caagaatata aagaatatta tacgaagggc tatcaaacag atattgcttc
    50701 aatcaagatc agtgagaaag agatggcatt tacgtttaag aatggaacaa cgaaacgttc
    50761 aaattatcgt tatgtaggga aagaaatctt gacctacgag gcaggaaacc gtggcgtaag
    50821 gtatctattc gaagcagaag ataaggaaag tggcgctttt agatatattc aattcagtga
    50881 tcacacgatt gcaccggctg actccgaaca ttttcattta tatgcaggaa atgaaagtca
    50941 agaagtattg ctgaaagaaa tggagaattg gccaacattt tatccagata caatggatgg
    51001 acgtgaaatt gcacaggaaa tgcttttgca ctgataggca aaaaagaacc gaagggtttg
    51061 gatcggctgg tcatttagtc ggtccaaact attcggttct ttttatgaaa tcaatttctt
    51121 taatccacgg atagaggctg ggatggtctc gccgtttgcc aacgtgactt ctctggtttt
    51181 tttattaatc gaccgcacat tttgtgtatt gataacatag gatttatgac aacggataaa
    51241 attccgatgc attttctcga tttctttgat cttctcatag aattgtaatt gggcatgagt
    51301 caaatggact tccagtttat gtggcgctcc tagcgaagta gaaaaatatt ggatgtcttt
    51361 gatcggtaaa aatttgatct catcattaaa ctctattttg aagatatctt tttggatttg
    51421 ggacatttga aaatcttcga aaattttgct caggatatgc tcgacatttt cttttaaatc
    51481 ataaagatct tctttgacga tataatcagt cggttcgatg ttgcttttta agattttcat
    51541 cgacatttcc gtatgggaag tcacatagat tatttttcct aaaggatcta attcacggat
    51601 caatttaccg atcgctacac catccatttc actgtcttct agttcgatat ctaaaaagta
    51661 aataccaaaa gaagaagttc tgatcttggc tgcattgaga agttcttccg gatcagaggt
    51721 agaaatatgg acataactgt ctaagttttc aatcatgatt ctatttttga tcaccgttga
    51781 taaaacttct aattgttttg gatcgtcttc acaaatataa atgggaatca tatcattact
    51841 cctttatttt atttgtatat aaatcgtttg agaaaaacgc tggttttcaa ttgatgtttc
    51901 aaggaaaaga taaggattgg tctttgtcaa ttcatccaag ataaacaagc caaggccttc
    51961 gttttttgga tttgtttttg ttgagaaccc tttttgtttc aactggttta acggtaatgt
    52021 atttgttgtt gtattgatca cacgaatcat cacggaatga ttcttcttga gtatggtcaa
    52081 ttcgattttt ttcttctttg cagcactagc tccttcaatc gcattgtcca agataatacc
    52141 aagcatacgt acaaggttta ccgtatgttt cgtatccagt tcgattggct caggaaaatg
    52201 aagctggacg atcaaccctt tttcctgtgc catcatgagt tttaatgaca gtaaactacg
    52261 gatctcagga actttcatat tatccaacat acttagattg gccggaaaaa ggttcatcat
    52321 ctggcgtgta ggtaatatcg tttgatcata ataagaccgt aatccttcta agtcgccttc
    52381 tgaaatatag ctgtgaagag aaaaaagcag attttggtaa tcatgtttga attctctcaa
    52441 gtcttgatag tttttttcta gatctttgat ataatcctgt tgcatcctga cttgttgctc
    52501 taataactga tcagctaatt tcttattaga gattttccaa taataaacca agatgatcag
    52561 gccaacacac agatatagaa tgaaaaaact cgtattcaaa gctaagacat caggtgtttc
    52621 accagaaaaa cgggtataaa aaataatgat atagtatgta aataatatca atgtcccgat
    52681 cgtaccgatc atacggcggt ccacagtgtg aaaccaagtc tctaacgatt tttttaaaca
    52741 aaaagctaat aaagcagata aaaaagcgga taagacaatg tgaaaatact gcaatgaatc
    52801 tcctggttca ataaccgaag atttcaaaac aaaaaaatcg ccgatgcttg ttaagtgatc
    52861 tcctaaaagc gacgtcaata acccaaaagg aagcgcgtaa accaattgtt gacgcgaagt
    52921 cgaaaagaag aaaaaagtaa gcatcagcat tcctatagca attagagcag taaaaatccc
    52981 taaaatcgaa tagacccatc caatcaaaat acaagaaatc agaatgactc ccgcaaacag
    53041 atatttgtat cggatgtgat ggtcaatcaa taagatacaa attgtgtaaa tcaaatattg
    53101 caacgctatg gaccataaaa acataaagta cagctcccat tgatttttac caaattatag
    53161 cacaattggc gtattatttt tttaattttt cagggatttt gaattcagaa ctcataccaa
    53221 agcagcagcc agatacaaaa aagtcagata agttagttaa ttgtttaatg attgtttttt
    53281 tcatgaaaga ttcctccaaa attcatcaag tagtttgtaa taggaagcaa cgtagctatt
    53341 tctaaaacaa tcccaagtag caaataggta gaaaagtaag aagctgagaa aaataacgta
    53401 atgagagaaa aaattagaaa catgctgatc gccttcttta ataatggggg acgtttctcg
    53461 ggttcaatgg gattgattgc tgtacctctt gatccatagc gaagcagtaa caaaaagcag
    53521 ctgattaaca aaaagaccat cattgggaag gagatagtca attgcagatg tttcatcacc
    53581 aagggaacgc cccataaata aagtagtgtc cacagcagac aaacgaattc agagtcggaa
    53641 tgcaatccat aagcatatct gcggatgcag atataagaaa gaaaggtgat tcctgtttcc
    53701 gcccatgagc ctgtcaccag agaaaagact agaacgaccg ctagttttcc aagattattc
    53761 aataaaactt ctaagacaaa ttttacttgg acatacacca tatcttcttc cgatttgtct
    53821 ccgctgaata aggcttctaa taatttgttc acaactttat ctttcatctc atacgtacct
    53881 ccaccacaaa ttttatgcta aaaaaaataa aaaacgtctt ttgtttcata aaaagtggtt
    53941 ttgacatcaa aaccgtcatg atctcctaaa aaaatcttcg ttttccaaga accttaccaa
    54001 aaaaagtgac agttttgata cgaaaagtac tttttatgac aagtttgtgc tttttcgagt
    54061 gatttatgtt aaaaaattac tgaccttata aaaggtgatg gagggattct tatggaaaat
    54121 gaacaattgt ttgaagtcgg cgaaacatat gcatttaaat ctgatttatt tgaatcacca
    54181 ataaaaggaa aaatagaaaa aatctatgaa aatagtgtga tgatacgtgt catgaaatgc
    54241 gcagtagaag acaatgatac cgctcataat ctgcactggc agttagttgt acgcaaatca
    54301 agtacaatag aagaataaaa acagataaaa tgttgagagc ataaagaggt tgtgagtctc
    54361 ccgtttagct ctgagttaca aggaataatg ttaaaaaaca gcttttcatg ttttatgaat
    54421 attgccttgt gaccgaggtg ttggcaggag aatactgttt atttgaaaaa agggctgcga
    54481 caagactttt gtcacagcct ttattttcat gaataggagt tacttttggc tacttttaga
    54541 gaagtagctt ttaaggagca gataaagtca aggcttttat ttctataaat gaagtagtaa
    54601 aatgaagaac agattttatt acaggaagga tgccgataat tgtgatcatt gttgcaggaa
    54661 cgatcggtgc agggaaaagc acactaactg aaatgcttgc ccaagattta gaaacaaaac
    54721 ctttttatga gaacgtggaa gataatgaag tattgccgct attttattct aatccagaaa
    54781 agtatacttt tttattacag attttctttt tgaacaaacg ctttttggca atcaaagatg
    54841 ctttcagcca tgatgacaac gtactagacc gctccatcta cgaagatagt atgctgtttc
    54901 atttgaatgc agatttaggg cgtgtttctg aagtagaagt aaagcagtat gaaggtcttt
    54961 tagaaacgat gctgaaggag ttagaagaaa tttcgccaca aaagaaacca gatctactgg
    55021 tttatattcg tgtatccttt gaaactatgt tagcgagaat caaaaaaaga ggccgggaat
    55081 atgaacagct agaacaagat cctgaactct attcctacta taaagaactc aatcgacgat
    55141 acgaagagtg gtatgaacag tttgatatat gtcccaaaat agtgatcgat ggggacaaat
    55201 atgatttcgt agccgatccg gcatgtggag aacaaatcgt acaagaaatc aagatgcggg
    55261 caaagaaaat gacagaagag gcagatgccc agtcgatccg ctacactaca aataacccga
    55321 aagcaaaggg aatctttaca actaaataat ataaaaacta agcaaattgc tcactcgcag
    55381 atatttctct gtgagcagca tcttgcttag tttttttgat tcattgagat caaatagtga
    55441 accgattcgt atgataaaat tgccataaat gaaatgtctg agtgagggcc atgatcaaaa
    55501 ataaaatgga aacgatcaaa atcgaatggg cgccaaacag atgatgacag aaaacaagga
    55561 caatagctcc tgcgataaag gcaagaacga cacttagata gtggaaaaat cgatcaaaat
    55621 ctgctttttc tttagaaata caataatcat ataagttcgt tgctacccct tttaaatttc
    55681 cagttgtaaa taaattagtg taggcacgcc cgttgatttt atcaaaagct acccactgga
    55741 tcgatgcagt aaaagcaatc aaaatcgtta ctaatgcggg atgctcatgg acgggtgtca
    55801 aactaacaaa gaaaaaaatc actgcttcat aatatagaac aaacaagcgc cagaaatgtt
    55861 tttctttttt tctaaaataa tcaattagat actttgttag aagaatccct aagaaaaaga
    55921 aaagggtaga caatactttt ttaccaacac ttagccattc tccgtcaaat gcttggatga
    55981 tcgatagaat gatattgcca gtttgagcag aggcaaaagc accatattgg atataagtat
    56041 aactgtccat ggctcctccg ataaatgtca gcaaaagtcc tattgtacgc ccctcgtgaa
    56101 aggaagtctg catttttttc atcacctttt atgaatattt ttgtcttcct tatcttagca
    56161 taaacgattg aaaaaaagga aaaattttca gaaaactaaa atcatatcgc ttcccatttt
    56221 catttttaga aaggtctgtt agtataaaga aaaaatagac aacgaaactt taaataacca
    56281 acttgaaata aacaaattca aagaagaaag atgggaagga aatcaatcag ttgaatgaaa
    56341 aagaaattct ccgattacaa aaagaattga aacgctggga gaacaagtat aaaaagcttc
    56401 aacaagtctt tcggctacaa gaagaaatca ttgctaccta tgaagcggct tatggcgaag
    56461 cacccggact tattgaggaa gaagtacaag taaaagaaag tgaaggaaac agctctttct
    56521 ttaaaatgat ggagaaacgt tcctataatc gcttcaatca agaacaaaaa gcagctatca
    56581 catatgatat gaagaaacat cttcgaatca tagcaggagc tgggagcggc aaaacgcaaa
    56641 caatctgtgc aaaagctgtt tatctgatga cgcaaaaaca agtagacgaa gaacgcatac
    56701 taatgatcac ttttacaagg aacgctgcaa atgaactaaa aaaacgagta gataatttca
    56761 gccagagaaa aaccgctgtc catatcggaa cattccatag tatctttttt aggatataca
    56821 atgaaatttg tcgaaaattt cctgaagtag caatgcaagg aatacaagga gacttttcaa
    56881 aggatactgc acaaaaagtg aatgctgttt tgcaacagct gatacgtaaa tataatcttt
    56941 atatatttga tcaatatgga gaaaaaacga ttgcttctcg gttagactat tggcagaata
    57001 tgaacctttc agtagaagaa atggttcagt tagttaaaga aaaatatgat tccattgata
    57061 agaatgcacg acagccgata tcagaacgac tgtatggatt gctcacggaa ctccaagaac
    57121 aaaaaagaag ccagcaattg ctagagttca atgatgtact ccaaaatctg aaaagcgctt
    57181 tggagaatga ggagatccag cattatatcg gccaaaaata tgattatcta ttcattgatg
    57241 aatttcaaga tactaatcca cttcaatggg aaatcgtgaa acggatgaca aaggaaaaca
    57301 ggatcaagtt gattgttgtt ggagatgatg atcagagtat ctatggattc agaggatcag
    57361 agccagcata catcaagaac tttgaaaaag aatatcctac caaaactctt ttcttattaa
    57421 ctaattatcg ttctcgggca gctattgtcc aaggagcaaa tagattgatc agctataata
    57481 aaaatgatcg gataccaaag gcgatgattc cagcacaaaa agaagcaggt gttatggaag
    57541 cctatttgtt ttctgatact aaagcagaaa gtcagtggct aatcaaacaa attcagacat
    57601 ttattcaaaa aaatgggaaa tataaggaat caatcatatt ataccgatca gcttcacaaa
    57661 cgcagcagct tgtccaatct ctgctgaaaa cgaatattcc ttttgtactg gaagcagata
    57721 gtccatatga aggtatcttt ggtatcaagt ctttccaaca tttctatcaa aaaattgttc
    57781 attggcaaac agcactgaat ctgcaaaaga aaaatcaggc ctaccagcaa ttgctccggc
    57841 agctgatggc agactgctac ctgaaaaaaa gtgaatgcga tcaatacttt tctcctaaat
    57901 atcaaaatat agccgttgca gattatattt tgtcgataag ccccaattta aaatcgaaat
    57961 ctgcagaaat ccgagagttt gaggaagcgt tgcgtaaaac agaccagcat caagaaatct
    58021 attcgcttgt tcaaagctat cttcatctgc cacgtatagc taaggaaatc gaccagagtg
    58081 atcaagaatg gatacgagaa gaagtaaagc agcatgctac tttcagaaga ctccaagcct
    58141 tatcgcaaga aacccaagcg acagctaaag aattaaaaac ccgcttgatt gcttacagac
    58201 aaggaaaact tgatgcacta tgtatccaga gcattcacaa aagtaaaggt cttagttacc
    58261 gaaatgtctt tttgattggt tgtaacgaag gaagtttgcc ttataacgga gcgacagaaa
    58321 agaaagtaat cgactttaat gaaataaaag cagaaccagt aacgacgata gaagaagaac
    58381 gaagactatt ttatgttgcg atgactagag caagaaatcg tctgtatcta tgtgtacctc
    58441 aaatgaaaaa ttcaagaaaa ttgaaagtat cgagatttat caaagaaacc gggagtcgtg
    58501 tgaaaaaagg aaagtgatag aagagagggt gtgacagaag tggtcagttt caagaaagaa
    58561 gaaggaatgt acgaaaattg cttctcaaat ttttgtgaaa tttcagctta ttttcgaaga
    58621 agctatttct ggaacacagt ttattcggaa aagggaggtg tgacaagaat tttgtcacac
    58681 ctcccttatt tattttgaaa atgagaatac tattatcaaa atagagaata tatagaaaaa
    58741 ttctcattat tcaaacgtaa ataatcagtt attttgattt tttttatatt ttattagcta
    58801 ataattggta ttttaaaaga aattactgta gaattggatt ataattaaaa aggagtgaga
    58861 aaatgataaa agtatatact tcgcctagct gtacttcctg tagaaaagca agagcatggt
    58921 tgcaagcgaa tcaacttgaa tttgaagaga agaatatttt tgccgaaccg ttaacagaaa
    58981 gtgagatcaa aaagatcttg gcagcgactg aaggaggagt tcaagatatt atttccactc
    59041 ggtcgaaaat ttacgaaaag ctgggcatag attttaacga attaacttta aagcaaatga
    59101 tcgtcttatt caaagaatat ccttcattat tgagacgacc tattttgatt gatgaaaaaa
    59161 gaatactaat cggatttaat gaagatgaga tccggtcatt tgtccctcgt gaaattcgat
    59221 gtgttgccag aaatctgatc tatttacaaa acaataacat cttaaattca tagggaggga
    59281 gccttcgtgg aagtgaaagt ctatggatca gcgcactgtg taacaagtct gaaggcgaaa
    59341 caatggttag aacaccatca attgccttat caatttgttg acttagaaga acgcgatcta
    59401 agtacagaag aagcagaaga attatgcagt tttgaagaag tgcaagcaga acagctattt
    59461 gcgacttggt ctgaggcttt tcacaacatt gcgattgacc tttcctgtgt aggaaaaaaa
    59521 caactgattt ttttatgcac acaacatacc aatctcctca gacgtccttt gattattatc
    59581 gatgaagttc tatttattgg atacaatgag cagttgatgg aacagcttat cttaaataaa
    59641 tagactttaa ctgttttaat caagtcactg agcatttcat acagttgtaa cagtcgaaca
    59701 cttagcatca agcgtgttat gtgttctttt tttgacttta tttttctact atagagacat
    59761 gctcaattta tgtggtcaaa attcaaatct aggtggggat atagagttaa aaatatattg
    59821 acatgtgtaa atttacatga tatattcatt tctgttgttt taagtagcaa agcaataaaa
    59881 tagtatttta aaaaacatat aaaatagttg ttgacatgtt agaaatgaca tgataagata
    59941 actgagttgt taaaaaagaa ccacttgtga agcttgattc tatcgaaaaa aacttcaaaa
    60001 aagttcttga caaataacaa atgataagtt atgataaatg agttgctgta agatttcgaa
    60061 aaacgaaatc aaaaaaactt ttaaaaaaga gttgacaatt acttgtctct ctgatatgat
    60121 ataaaagttg ctacggcagc caaaaacgaa tcggggaaca ctcctgatga agtagacctt
    60181 tgaaaactga acaaagtaag acaaaccaaa tgtgtagggc gtcttgattc aattcaagac
    60241 aacaaaca
//
LOCUS       DASHRQ010000012        58472 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.012, whole genome shotgun sequence.
ACCESSION   DASHRQ010000012 DASHRQ010000000
VERSION     DASHRQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.012"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(183..1502)
                     /locus_tag="U2M88_000808"
     CDS             complement(183..1502)
                     /locus_tag="U2M88_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HEM8373208.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            1759..2229
                     /locus_tag="U2M88_000809"
     CDS             1759..2229
                     /locus_tag="U2M88_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEM8373209.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2451..3554
                     /locus_tag="U2M88_000810"
     CDS             2451..3554
                     /locus_tag="U2M88_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEM8373210.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            3775..4734
                     /locus_tag="U2M88_000811"
     CDS             3775..4734
                     /locus_tag="U2M88_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM8373211.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            4904..5584
                     /locus_tag="U2M88_000812"
     CDS             4904..5584
                     /locus_tag="U2M88_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEM8373212.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            5674..6708
                     /locus_tag="U2M88_000813"
     CDS             5674..6708
                     /locus_tag="U2M88_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEM8373213.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            6722..7057
                     /locus_tag="U2M88_000814"
     CDS             6722..7057
                     /locus_tag="U2M88_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373214.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            7041..9521
                     /locus_tag="U2M88_000815"
     CDS             7041..9521
                     /locus_tag="U2M88_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373215.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTEANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            9577..11136
                     /locus_tag="U2M88_000816"
     CDS             9577..11136
                     /locus_tag="U2M88_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8373216.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            11133..12497
                     /locus_tag="U2M88_000817"
     CDS             11133..12497
                     /locus_tag="U2M88_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEM8373217.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            12668..13738
                     /locus_tag="U2M88_000818"
     CDS             12668..13738
                     /locus_tag="U2M88_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8373218.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            13753..14421
                     /locus_tag="U2M88_000819"
     CDS             13753..14421
                     /locus_tag="U2M88_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8373219.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            14441..15019
                     /locus_tag="U2M88_000820"
     CDS             14441..15019
                     /locus_tag="U2M88_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM8373220.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            15420..16331
                     /locus_tag="U2M88_000821"
     CDS             15420..16331
                     /locus_tag="U2M88_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEM8373221.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(16709..17626)
                     /locus_tag="U2M88_000822"
     CDS             complement(16709..17626)
                     /locus_tag="U2M88_000822"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEM8373222.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            17828..18265
                     /gene="zapA"
                     /locus_tag="U2M88_000823"
     CDS             17828..18265
                     /gene="zapA"
                     /locus_tag="U2M88_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEM8373223.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            18323..18871
                     /locus_tag="U2M88_000824"
     CDS             18323..18871
                     /locus_tag="U2M88_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEM8373224.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            18986..21346
                     /locus_tag="U2M88_000825"
     CDS             18986..21346
                     /locus_tag="U2M88_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEM8373225.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            21433..21747
                     /gene="trxA"
                     /locus_tag="U2M88_000826"
     CDS             21433..21747
                     /gene="trxA"
                     /locus_tag="U2M88_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEM8373226.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            21929..23707
                     /gene="uvrC"
                     /locus_tag="U2M88_000827"
     CDS             21929..23707
                     /gene="uvrC"
                     /locus_tag="U2M88_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEM8373227.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            23852..24751
                     /locus_tag="U2M88_000828"
     CDS             23852..24751
                     /locus_tag="U2M88_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8373228.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            24744..26006
                     /locus_tag="U2M88_000829"
     CDS             24744..26006
                     /locus_tag="U2M88_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8373229.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            26167..28065
                     /locus_tag="U2M88_000830"
     CDS             26167..28065
                     /locus_tag="U2M88_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEM8373230.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(28395..29114)
                     /locus_tag="U2M88_000831"
     CDS             complement(28395..29114)
                     /locus_tag="U2M88_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEM8373231.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            29451..29783
                     /locus_tag="U2M88_000832"
     CDS             29451..29783
                     /locus_tag="U2M88_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373232.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            29770..31773
                     /locus_tag="U2M88_000833"
     CDS             29770..31773
                     /locus_tag="U2M88_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEM8373233.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            31792..32262
                     /locus_tag="U2M88_000834"
     CDS             31792..32262
                     /locus_tag="U2M88_000834"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEM8373234.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            32283..32867
                     /locus_tag="U2M88_000835"
     CDS             32283..32867
                     /locus_tag="U2M88_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEM8373235.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            32882..33868
                     /locus_tag="U2M88_000836"
     CDS             32882..33868
                     /locus_tag="U2M88_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEM8373236.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            33858..34169
                     /locus_tag="U2M88_000837"
     CDS             33858..34169
                     /locus_tag="U2M88_000837"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEM8373237.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            34191..35972
                     /locus_tag="U2M88_000838"
     CDS             34191..35972
                     /locus_tag="U2M88_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEM8373238.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            35965..37341
                     /locus_tag="U2M88_000839"
     CDS             35965..37341
                     /locus_tag="U2M88_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEM8373239.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            37348..37983
                     /locus_tag="U2M88_000840"
     CDS             37348..37983
                     /locus_tag="U2M88_000840"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEM8373240.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            38013..39368
                     /locus_tag="U2M88_000841"
     CDS             38013..39368
                     /locus_tag="U2M88_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEM8373241.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(39560..40954)
                     /locus_tag="U2M88_000842"
     CDS             complement(39560..40954)
                     /locus_tag="U2M88_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEM8373242.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            41046..42017
                     /locus_tag="U2M88_000843"
     CDS             41046..42017
                     /locus_tag="U2M88_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEM8373243.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            42014..42796
                     /locus_tag="U2M88_000844"
     CDS             42014..42796
                     /locus_tag="U2M88_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEM8373244.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(42965..43336)
                     /locus_tag="U2M88_000845"
     CDS             complement(42965..43336)
                     /locus_tag="U2M88_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEM8373245.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            43470..43913
                     /locus_tag="U2M88_000846"
     CDS             43470..43913
                     /locus_tag="U2M88_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEM8373246.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(43956..44765)
                     /gene="yidA"
                     /locus_tag="U2M88_000847"
     CDS             complement(43956..44765)
                     /gene="yidA"
                     /locus_tag="U2M88_000847"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEM8373247.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(44784..46151)
                     /locus_tag="U2M88_000848"
     CDS             complement(44784..46151)
                     /locus_tag="U2M88_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEM8373248.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(46135..46965)
                     /locus_tag="U2M88_000849"
     CDS             complement(46135..46965)
                     /locus_tag="U2M88_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEM8373249.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            47128..47565
                     /locus_tag="U2M88_000850"
     CDS             47128..47565
                     /locus_tag="U2M88_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEM8373250.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            47641..48267
                     /locus_tag="U2M88_000851"
     CDS             47641..48267
                     /locus_tag="U2M88_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEM8373251.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDEDTDLTEEDDDDLFDDDDDEDEEIAAYNS
                     DLQEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            48456..48962
                     /locus_tag="U2M88_000852"
     CDS             48456..48962
                     /locus_tag="U2M88_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEM8373252.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            48979..49878
                     /locus_tag="U2M88_000853"
     CDS             48979..49878
                     /locus_tag="U2M88_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEM8373253.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            49965..50849
                     /locus_tag="U2M88_000854"
     CDS             49965..50849
                     /locus_tag="U2M88_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEM8373254.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(51041..51112)
                     /locus_tag="U2M88_000855"
     tRNA            complement(51041..51112)
                     /locus_tag="U2M88_000855"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51077..51079),aa:Arg,seq:ccg)
     gene            51172..52506
                     /gene="rpoN"
                     /locus_tag="U2M88_000856"
     CDS             51172..52506
                     /gene="rpoN"
                     /locus_tag="U2M88_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEM8373255.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            52655..53746
                     /locus_tag="U2M88_000857"
     CDS             52655..53746
                     /locus_tag="U2M88_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEM8373256.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            53787..54788
                     /gene="gap"
                     /locus_tag="U2M88_000858"
     CDS             53787..54788
                     /gene="gap"
                     /locus_tag="U2M88_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEM8373257.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            54898..56088
                     /locus_tag="U2M88_000859"
     CDS             54898..56088
                     /locus_tag="U2M88_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEM8373258.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            56192..56947
                     /locus_tag="U2M88_000860"
     CDS             56192..56947
                     /locus_tag="U2M88_000860"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEM8373259.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            57063..58361
                     /gene="eno"
                     /locus_tag="U2M88_000861"
     CDS             57063..58361
                     /gene="eno"
                     /locus_tag="U2M88_000861"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEM8373260.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        19397 a        10179 c        12340 g        16556 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaattaaa agtatgaatt attcaggtag aatctctgaa gaataaatca
     1681 cgtgatcgtg acgataaaat acacttacgt caggtgataa aaaatacata agtagaaaac
     1741 taagaggagg aacagaaaat gaaagaagtt caattgatca gaaaaagcga actatcagaa
     1801 ggtggttgca atgcatgcgg cgtagtagaa gcaacaagct atacattaaa gcttggtgcc
     1861 aacaaagcga taatctctga gttgacagta ggcggtcttg tggacagtct ggcgcttgct
     1921 gaaggtttta ttggagaaga tatttatgag atgttttcag aaatccgtca gttaaaaaaa
     1981 ggggaaaact gcatagaggt tcatcatgag agcccgaatg tgagattcaa acgtggtgat
     2041 aatgagatga tcttcaataa tcatgtgtcc gatcacaccg agctatatgg aatcgtgaat
     2101 caaatactaa ctgagctttt tggattggga ccttatgcgt tcaaggaaga aaacggtaat
     2161 ccaaaattaa acgaagaatg gcaagaaacg attgaaatac aaagaaacaa cccgcactta
     2221 tttcaatagg catccacagt atttcgtaaa agggactttg acacgacttt tgtcaaaatc
     2281 ccatcgtttt ttatctattt gtgttctaaa catttctttc agagtagtaa caactggaat
     2341 gactgaaatg taaacagatt atttgcctgt atcaatacgg aagagtaata taaagtgtgt
     2401 aaacgctatc atattattca ttcaattttt tatagaggga aggcattgaa atggaaaaaa
     2461 cgtatcaagc aggtacaaat gaaggaatag tggattttat caatatggaa gatttagaaa
     2521 tagccgcttc acaagtgatt ccagctggtg gttatggtta catcagcagt ggtgctggcg
     2581 acttatttac ttatcaagaa aatgaacggg cattcaatca tcagctgatt attcctcatg
     2641 tattaagaga tgtagaatta cccgatacaa ctacacattt tgacaaagaa acgctgactg
     2701 caccaatcat catggcacca gttgctgctc atggcttagc tcatgtgaag gcggagaaag
     2761 cttctgcaaa aggtgtagca gattttggga cgatctatac agcaagttct tacgcttctt
     2821 gtacattaga agagataaga gaagcaggcg gggagaaagc accgcaatgg tttcaatttt
     2881 atatgagcaa agatgatggg atcaatttgg atattcttga agtggccaag cgtaatggag
     2941 caaaagcaat cgtattgaca gcggatgcaa cagttggcgg aaaccgtgaa acagaccgca
     3001 gaaatgggtt taccttccca ctgccaatgc cgatcgttca ggcttatcaa tcaggagtag
     3061 gccaaacgat ggatgctgtg tacaaatctt ccaaacagaa gcttagtcca aaagatgtag
     3121 aatttattgc tgctcattct gatttgccag tctatgtgaa aggtgtccag tcagaagaag
     3181 atgtctatcg ttcattagaa tctggtgctg gcggaatctg ggtatctaat catggtggcc
     3241 gtcaactaga tggtggtcca gcagcgtttg attcgttgca atatgtggca gaagcagtag
     3301 ataaacgtgt accaatcgtg tttgacagcg gtgtacgacg cggacagcat gttttcaaag
     3361 caatcgcatc aggtgcagat cttgttgcta ttggcagacc cgttatttat ggattgtctt
     3421 taggaggaag tacaggagtc catcaagtgt ttgatttctt taaaacagaa ttggaaatgg
     3481 tcatgcaatt agctggaaca caaacagtag aagatatcaa aaaaataaaa ttgcgtgaga
     3541 atcgttttat ttaagaaaca aatgacgaaa aaaataggag tgcggcagat gatgccgtac
     3601 tcctattttt atagttcaag gatgtcacta atagaataat ttgaaacgaa aaaataaaat
     3661 tgaaatattt tttcatactt cgccgattgt aaaattaagc tagacaacta aaaaaagtca
     3721 tttgtgctac actcaaaata gttccgacga aagaattata aggagtgagt acaaatgacc
     3781 tataaccatc ttacaccaac tgaactcgta atgatagaag cttatttcaa tcaatcacaa
     3841 cctgtttcaa aagtagccaa tctgttacaa cgttctcgac aaactattta taaggtctat
     3901 cgttttctta aatctggtgg aagcgcaatg agctactata aacaatataa gaaaaacaaa
     3961 cgtaactgcg gaagacatcc gattgtttta cctgatgagc aacaggaata cattcaaaaa
     4021 aaggttgttc aagggtgggc tcctgatgtc cttattggtc gagcagagtt tcctattgct
     4081 tgttcggtac gcacactgta tcgaatgttt aaagataaaa cctttgacac tcacgattta
     4141 ccaatgaagg gaaaaagaaa accaaacggt cataaagaaa aaagaggaaa acaagggttt
     4201 cgccggtcaa tccgagacag aaagacagac tatacccagt ttgatcatga atttggtcac
     4261 ctggaaggtg acactatcgt gggtggaaaa cataaaagcg cggtcatcac attagttgaa
     4321 cggctatcta aagtcatcat taccttgaaa cccgaaggca gacaggctaa agacattgaa
     4381 aataggctga atcaatggtt tcgttgtgtc ccaaaaaact tatttaaatc catcacattt
     4441 gattgtggaa aagaattctc aaattggaaa aagattagta ataccaacga tatttcaatt
     4501 tattttgcgg atccaggaac accttctcaa cgaggattga atgaacattc aaatggctta
     4561 ttacgtaaag atggtttgta taaagctatg gattttaatg gtgtatctga aacctttatc
     4621 caatcggtgg catcgaaacg aaatcatatt cctagaaaat cattacacta tcggacacca
     4681 atggaagtat ttttgagtta cgtcacaaaa gatgatttgt ctaacttaat ttgacaaacg
     4741 aaacttaata aaaaaggaag aatattcatg taaacgattt tattgttttt ttctctttta
     4801 tacaaaaagg gtcataattt caagtaagcg gatgaattca tgaggatttt ttgcatactg
     4861 tctctactat acttaggtca ctaagaaaaa ggagtgattt attatgaaaa agaacatgat
     4921 tgggacatta ttactttcaa cacttcttct aggcggcttg gcaacaccag cttttgctga
     4981 gggtcaagcg acttcaaaag gagatattac ctttactgaa ccaacaaata ccgtggagcc
     5041 tcttaaccct actgatcctt caaaaccagt agagccagca gatccggaaa atccagcaac
     5101 tggccagaca ggtcctttaa cactggatgt agttcccgaa ttgccatttg gtacacacga
     5161 gattgaaagc ggaacaaaaa cgtatcaagt agacgcttca aaaaacgata caccttatct
     5221 ccaagtatca gatcgtcgag gtgtaggtgc agatggccaa gcacaagggt ggaacgtgac
     5281 agtttcggtt tcagattttg taaacggttc acaagttttg caaggcgctg aattagattt
     5341 tggaacatcc actgttaaaa gtacgtcaga taacgaatcc actggaccaa ctagtcaaac
     5401 tgtaactggg ttgagtaaag caagtgcagc gacaccaata tttacagcag caaaagatca
     5461 aggtctggga acatggcttt ctgtttatga tccagcaaat atcacactga aagtgccgaa
     5521 agcagcagct ggaacattca cggctgattt gacttggaac ctcgtggctg gcccagtagc
     5581 atagagtatg aaataaattc aacaaactgc ggtgaaaata ctataggtga aggatttttt
     5641 accgcagttg ttgttgaaag gatgtggtgg acaatgaatc aaagtaaacg actgtttgtt
     5701 tcgttttttt ctattttaag tctatttttc atttttccgt caatcagcca agcaaaggat
     5761 tcagcaggag actttggaat caaaccggta tttccagaga accaaataga taaagcaatc
     5821 ggttattttg atttattagt ggcaccggaa caaaatcaga cactagaagt aattatcagc
     5881 aattcttcag atgaagaacg aacatttgag gtctctgtca atccggcggt aacgagtgat
     5941 ggtggaacga tcgattatag tcaaaaaaac ccaacgttag atgagacatt gccgtttgat
     6001 gtccgtgacg ttttgctcat tgcgaaaaag gaaatcaatg tatcagcaca tgcagaaaca
     6061 actgtaccta tagaagtgaa aatcccagca aaaagcttta agggacgtgt attagcgggt
     6121 attcatgttt cacctaaaga agaagctgaa acagaaaatg ctacagaagg agcacaaatc
     6181 aaaaatcgta ttgcttataa tctcgcagtc gttttacaag aaagtcaaga gacgatagaa
     6241 ccggatctga aacttttatc aggagatttg gacgaggtca acgcaaagcc aactgttcag
     6301 cttcgtttcc aaaatcctca gcctaggatt atcagcaatt tgatatttac gagcaagatc
     6361 ttttatgaaa atcagcttta tattgaaaat acgtctaatg catttttagt tgcaccaaat
     6421 tcgaattttc acttgaatct tgatttagct ggtgataaag caaaagccgg cgattaccgc
     6481 gcagaaatca tagcaaaatc aggcgacagt aatgaatggc gatttacaca aaactttacg
     6541 atcaaaaaag aaaaagcaca aaaagtaaat gaaaattccg tttttgcagt acaagaacaa
     6601 tctttcccat ggctttatgc aggattggtt gctgcagttc tagtgttatg gttgattttg
     6661 ctgtttttgt gggtccacaa gaaaaagaaa aaaggagaaa caacatgatg aggtgatttc
     6721 gttgaaaaca tcgatcttat tgggagggat tattgcaagt ttgctgttct tcagctggca
     6781 agagccagtc caagcagata ccaaaagttc aggtacgatt acattttatg atctaagtcc
     6841 aattccgcat ccaagtggtt cagtcaatgg cgcatcaaaa cagcagacac agctaccgat
     6901 ttataaagaa tctaatcaac atggcgtatt cccggagacg aatgaacaaa agatccgttt
     6961 tctttatttg atcattggta tagaattatg gcttcttgcg tggttgttgt tatggagaag
     7021 aaaggaaaag gaggatgaac atgttgctac aaaataaatt ttcatttttt cgctccttct
     7081 tttttacttc gctttttgtt ttaagtatat ttatcttttc accttcggca gaagctgttg
     7141 gagcaacgcc tcctgccatt tcttatgatc catacacgta tgaaaatatc gtacctccaa
     7201 atggtggacg tagtgcagtc aatggcggga agcttgtcca actaactgag gctaaccaat
     7261 tgcctaaatt gactgctact tatttgaacg atggttcaaa tgatccttgg aaaggtggca
     7321 cggtccgttt tcattgggaa agccatgtga aactaacaaa tgtacttgat ttggtgaata
     7381 tcactgcaca aggatttgca tttgtggcga tcatcaattt gccggaacat gtatcaagca
     7441 gtgatgtatt aaaagcaatt gactggaaca acgcctactt gtatattgcc ggtgccccca
     7501 tcaaggttac tagcggaagt ttattatcag caggaagcca taccttgcgt tttgggatgg
     7561 ggtcgtggga tacaaatact ctattgacag atattattgc tattattacg cgaggcgaat
     7621 tcaacgtgga cgatatcccg cttcaatttg atgttacagc taatgtcgcg aagatgacag
     7681 aaaacggaga taaatatgat acgagtccaa acaaaatttt gaccaagggt aaattacaac
     7741 cttctatgtc taaaaaggcc gttttttctg ttgattttta tgattcgaat aatattatcc
     7801 aagatcgtat gttcaattcg attttagctc cagctgcagg tagattttat cctaaattaa
     7861 ctggcggaaa cttgaatcca ggagcatatg cattttctgc aagtgcagag ataaatacgt
     7921 ggaatagtta catctcacca agtgattcaa caggaaaata taatacattt gctgatacca
     7981 cagaatacgt ggatggaaat aaccagaacc ttccaggctc taccaatagt cggacattga
     8041 cgatggggct gactgaagga cgttttgatc agttgccatt tagtcgcttt gatcgcgtag
     8101 tcaactattt tacaggaaaa aacgttacac aaggagcaat actgactcat actccagcaa
     8161 gcacaagtgg attgaatcag acagccaata tcgtgtacag tgggaaagat gcgcaaggcg
     8221 gattgctttc tcctgttgct ttaaaggtat tggagcagta caagctagac ggaacggttt
     8281 taccaacaaa cttaacagcc gatagtcagt ggggaaagct cctttcttct gcatcgtacc
     8341 aagtcacggc caattggtca gcaactgaac taaaaaaggg ttcgatccat tacaggcttt
     8401 attcgaaagg aacccaaaaa ttagcgacag gatggacaga tcaacttttc caaacgatcc
     8461 aatcaaataa cgggggtgta aatacagcta cttctacact acctgcattg gcttcagggc
     8521 aatattattt tgattatcgc ttggtggacg atgtacttac taacatgtat ccgtcttttg
     8581 cgtacaaatg gcagtctgaa caagctggaa tcaccactct tcctcaaata acagtggcta
     8641 attttccatc tgtgagtgca gatagtcagc taaaaaacct tagcagacaa cctgagacag
     8701 gagatcccct gtctgctcta tcaggagata cgattcaaga aaatgtgaca ttcacgttaa
     8761 caaaatctgg ggatgcgata accgacaaaa aaatcacgat ctcacttcct gaaaatacta
     8821 cttatatcaa tggttcctta aaattaaatg gtacaactat cgcagatacc ggcattcaac
     8881 aaggagtttc tgtcccagca gatctattga gcaaaatagg agataccatt catcttactt
     8941 acaactatca attaaatact gtagatactt ctttacaatc cgtcagcata ctgacaaagg
     9001 cagctgtact ttccagcaat attacattag cagatggtgc taagcttcct aacccagttg
     9061 tccaaacatc tgctaaaaca attcttgtcc ccaaacaaga attaacatta gttaatgtac
     9121 cagatgattt tacatttggt aacgatctcc ccaaaccgtt gaagacaagt tattatgaat
     9181 caaaaggcga tttttctttt gatgtgcggg atactcggct tccaagcacg tctccttggc
     9241 aactgacagg tacattgact tctttattta agaatgatca aggtcaagaa ttaagcggaa
     9301 caaaattata cttcaatcat tctggatcaa agcaattgat tcagcaaggt caaaacacgt
     9361 tgatttatga gagtgatgga acagctaaag gagaggttct cgttgacttt cccgataccg
     9421 atggtttact cttagaggta aatagcagta cgaatgctca acctggcgca acgtatcaag
     9481 gcatggtcac atgggaactg acagctgggc caaccagcta agtgattaga atttgtatgg
     9541 atctgtgata actgtcacag atcctctttt tcaaggatga gaaggaaact tctgctgtta
     9601 aaatcagaaa tgcttgaagg agtggacttg gaacttttca gtttgcttga aaaaaatgaa
     9661 caacggcaaa tcaaactttt ccaactttta ttagaggaaa aaggagaggt ttccgttaat
     9721 aacgtactta cccgcttatc agttgatcgg gctaccttga aagaagatct gttttattta
     9781 caagctaact tggaaaatat caaacaattg gaaattgtta tccaagaagg gaatatttta
     9841 ttaatcagac atggaaatat agcagcagtc gatgtttatt atcattgtta cttgcgctat
     9901 tctacaaaat atcagatact tctttatctg ttggaaaatg gctcatttga tcggcagaaa
     9961 cttgtcatgg aacttaatat cagttcggct actttgtctc gcagagtgaa ggaattaaat
    10021 gattctttaa atgagtttca tctgaaaata aaaagcgggc ggctgaccgg tccagaaagt
    10081 caaattcgtt atttttattt ccaattattt tggtttggac gtccctatct tgaaaacaaa
    10141 aaagaataca gtgatttatc aggaaacact tttttagatc tgctgcaaaa agagtttagt
    10201 tttgctatta cagaagaagg acgattgaaa tttgaaatat ggctttccat tatgaagaag
    10261 cgaatgcgct gtacgaaaaa atccaaacca gattatagca atcttcctgt tggattgttt
    10321 caaacgacac aatttttatt ggacctccat acatttttga cccgttactt ctttcaatat
    10381 gcctttcctt ggaatcaaaa tgaaacgttg gttttttatc ttttcatgat cagtaacttt
    10441 acattagata cacataactc gcatgtccag gagtttttga taagcgaaaa atacaaagac
    10501 gacgtagtag ctcaattgaa tcgaacgttt aaacaatcga tagaagaata tttctctttt
    10561 tatgtttttc cagaagagat ttcgcaaaag attgcgttga cagtgatgca aaatcacgct
    10621 cattttgcct attttagagg atggatgaat atttttggcc aagaaggatt aaaacagcgg
    10681 ttaactgaag tagaagataa acaattcctg gttttatgtg aaagattggt cagacaaaca
    10741 tttgctattg ttgattttac tgaagtagac gaggaaaatg cccgctttga attatttgct
    10801 cgctatgcat ctattttacg gacgctgatc aagcagttag aattacgtct gcaaattgct
    10861 tatgatcttc cttatgaaag gctaatggcg gatatcgtga aagaaatcat ccaggaacaa
    10921 ttgctgaata cgatcaaata tgatttagtc gaatacgaga aagaaaagca gtatgatatc
    10981 attttgacta gccaattgaa agagtacccg tcacagaagt ccgctaaagt ttttgtattc
    11041 actagcaacg aatataatta tgatttcccc tatttgaacc aatttttaaa agaatgttat
    11101 ttagaaaaat tgaatttaag gatgaataaa acatgaaaat cgtgatcatt ggtgcttccc
    11161 atgaaggcat cacagctgcc ctaaatctaa gaaaacttca accgaaagca gaagttttac
    11221 tgatcgatga agatcataaa gatggactag gctatgtttc aaatggaatc aacctttatt
    11281 taaaaggaaa gatacgtagt ttagctgaag ttgcccataa catgcggact cttcaagaaa
    11341 gtggggcaaa actgatcacc gaatggagag taactgaact tgatccagac aaacatcagc
    11401 tcatcctcgc atccaaagaa ggaaaaaacg agaccataac ctatgataaa ttaatcgtag
    11461 cgacaggttc ctctcctgtc accttgtata aacaaattga agctgaaaat gtctacacct
    11521 ataaaaatct agtgcaatct aagcaagtac ttgccgcttt aaaagaagca aaagaagtag
    11581 tgatcttcgg tgctggttac attggattgg aattagcaga tgctttgaga aataaaggat
    11641 acattattca tctagttgat tatatgccga atgttttatc aagatatttc gataaggata
    11701 tgatcaacag ttttcaaaat cagctgcaaa ctaagcaaat aaatttttat ccaaatgaat
    11761 tcttgatcga ttggaaaaaa tcagaagaga aagtagtatc cgtacaactt ctgtctcaaa
    11821 caataaaggc agatatggtc attttttctg cccaaactcg tcctaatacg acattgttaa
    11881 aagaaaaagt ggctttgtat gaggatgaga ctgtgatagt caatgaatat ctgcagacaa
    11941 gtgatccgga catctatgcg attggagata tcgtcccagt cagttttgac aaaaataaac
    12001 ggcatctctt tttaccttta gttactcgag ctgtacatat ggcaagagca gtagcgctaa
    12061 ctttgagtgg tcagccaacc gcttacgacc tcagacaaaa gataacagca gcagtcatta
    12121 tagactactt tttaggaact gtagggctga cagaagatga agcgccattc ttggaacaaa
    12181 ataccggttc gtgttcagga gaatttgatc tatttccaca atacgacgaa gaaaacaaaa
    12241 cagtaaatgc aaagctgatc taccatccgg atacattaga aataattggc ggacagctga
    12301 taagccaaga attcttatta tcagatttga acctgcttgc ggatatcgtg aaacacaaaa
    12361 ctacgattcc acagctagca gttgaggatt ttggatttct gtctgaatac acctctcgtt
    12421 ttcactacct caatgagtta gcttttaaag tactagctaa gagagcaaat agcaatagag
    12481 tagataaaag actgtgagaa atatcacggt cttttattgt gtggggatat aataaatatt
    12541 aatcaatcga gaataaaaaa ttcatataat gaaatagcgg aagaattttt tgagcataat
    12601 attcgccaaa aatttatcaa gtgataaaat tgttgtataa aatgattatt tcaggaggag
    12661 gagttaaatg tttttagcat taaatgaaat tatgcattca aagttacgtt atgcgttagt
    12721 tgcaggggtg atgtttttga tcgcctacct ggtatttttt ttgactgggt tagcttatgg
    12781 cttagctcaa gataatcgaa cagcagtcga caagtgggag gcagattcga ttgtgctttc
    12841 gaaagatgca aattcaaatc tagggatgtc catgattact aagaagatag cagaagaagt
    12901 cgaaggtggg aaagtggcgt atcttgccca aacacctgga gtcgtgacta gtaaagacag
    12961 tacagaagaa ggaaaaatca atgttagctt cttcggtatc gataaaaatc aattcatcat
    13021 gcccaatctt gttgaaggaa aagcttttga taacgatgat gaggcagtag gcgatattag
    13081 tttgaaagaa gaatatggtc tagccatagg agatacagtt aagttatctg gtagtgacaa
    13141 gacgtttaaa ctaactggct ttacggatca tgccaaattc aatgtctcac cagttctcta
    13201 tacaaccatc aatgcttacc aagaaatccg cttcgaaaaa gaagatacaa gtgaaaatgc
    13261 acggattaac gcaatagttg tcagaggtaa aatcagtaat ttacctgaag atttagaaca
    13321 gatcaaaatt tctaaattca tcaatgaatt acctggatat aatgcgcaag tattgacttt
    13381 tggatttatg atcggatttt tgatcgttat tgcggctatt gtgatcggga ttttcattta
    13441 tgtactgacg atgcaaaaga ttaatatctt tggtgtaatg aaagcacaag ggatcacagg
    13501 aggatttatt gcgcgttctg tagtggcaca aacattcatt ttatcatttg ttggtattct
    13561 tcttggtcta ttaggaacag tgggaacatc ccttgttcta cctgatgcgg ttccattcca
    13621 aagcaattgg ctgttttttg gcgtcattag cttgttgatg ttagtagtag ctgtgttagg
    13681 cgcactattt tctgttcgga caatcgtaaa aatcgatccg ttgaaggcaa ttggataaga
    13741 aggaggaaga acatgaaagc aatagaattt aaagaagtag aaaaaagttt ccaagatggc
    13801 gaccaagtag ttaaagcatt gaaagaaaca accatgagta tcgataaagg ggaatttgtt
    13861 gcaatcatcg gtccttctgg ttcaggtaaa agtacttttt tgactttggc aggcggattg
    13921 cagacgccta gcagtggctc tgtatttatc aatgatcaag aattcagtaa tcaaaaagaa
    13981 agcgaacgcg ttaaactacg ttttaaagaa attggcttta ttttgcaagc ttccaatctg
    14041 gttccttttc tgacagtaaa aaagcagtta ttattagtag ataagattaa acatgaacat
    14101 cgtcaaaaag aagcaaacga attatttgag cagcttggcg tggataagtt gatcaacaag
    14161 tatccagaag aactatctgg tggagaacgg caaagggtcg caatcgctcg tgcattgtac
    14221 aatgatccgt ccattattct agcagatgaa ccaactgcta gtttggattc agaaaaggca
    14281 cgagaagtag tcgatatctt ggcaaaagaa tcaaaagaca aaaataaagc aacgatcatg
    14341 gttactcacg atacacggct gattgacaaa tgcgatagaa tatttatgat ggaagatggt
    14401 gtattgaaag aaaaagaata accaaaaagc aggtggaaaa atggtccatc gatacaacaa
    14461 agatacacag caaaaaattt tgaaagaagc gactgacttg tttatgtcca agggatatct
    14521 cggcacttca acaagagaaa tcgcacaaaa agttgggatc acccagccca atctctatca
    14581 ttatttcggt gataaagaaa aactatatac ggcagtttta gaaaaccact taaaagatgt
    14641 tggaaaagct ttgagagaaa tcgttcaaac aagcgaaacg ggatttcaag taaccttgac
    14701 aaaaatggcg cagtttttga ttgagacaca tctggtcgat ttgtttctga tgcttcacga
    14761 tcttgaaagc aatctttcaa aggaaacaag agatcatctt ttctttctat ggaagaaaaa
    14821 ttatcgtgaa ccttttgaag aaatcttttc agcaaaccaa tctgtgctaa gaaatgggat
    14881 ttcaaaagaa atcgcagcaa ggcatttttt cttagtgtta gcaccttaca tcactcggtc
    14941 gaaaagacct gtggaagaac cgctaaatgt ggcgcaattg atcgatttat atttgaatgg
    15001 tgtattagga gaagcgtaaa gctgttatat agggagaaaa tagttaggtg aagaagcaag
    15061 atatgacgaa gatcgttata tcttgcttct ttatataggt agtttttcat aaatacaaat
    15121 ttgttattct ctcatgatta tcagggtata tttttattta ttatgtcggt gttccaggaa
    15181 gtgttttaat ttaataacga agaaattaat tgtttgtaga atgaaggaaa ctatttattg
    15241 ggtagatgaa tcaccacgaa tagtcaaaag gagatttcag gatatgccaa aaatgaaaaa
    15301 aatcatttta gcagtaatgt tattttctac ggttatatct tttacagccc cttttttgct
    15361 tacttaagct gattttaagt tttggccctt agtctggatc tacgaggtga gagtcaagaa
    15421 tgaaaaaaat aattggagct tttgtatttg caatcattat cattattatc tgttttcaaa
    15481 tagatgtttt tggtcaaaaa aagatagttg gacaaacggt cagcagtacg aacagtacgg
    15541 ttacaaagac aaaggaatca tttgatgata cagaacaaga gacattaatc aaaatcggaa
    15601 aaaatgaccg tacactatat acagatgact cgctaactac cccacttgca actataaacg
    15661 gcggagagct ggcggagttt ctttctgaaa caaaaaatgc ttatcagatc aagacaaatg
    15721 atggctatac tggatattta gccctcgaag atggaaccaa agtcacaaag aatatacaaa
    15781 caaaaccgga agagctatct gatgcagtga ttgtattaga tcctgggcat ggcggaaatg
    15841 atactggggc attaagcaat gatgaacaaa cagaagaaaa agatataaca ttaagtacag
    15901 ccataaaagt aaaaaaggcg ctagaggatg ctggagcaac ggtttatttg acccatacta
    15961 cagatgaatt agtacagctt ggagatatat gcgactacag tgaagaaaaa aaggcagatg
    16021 tctttatcag tttacacgca gactctacgg aatatgcgaa tgaagcaact ggaatcacga
    16081 catattatta ctatggacaa gaggaaacat tagcacagac aattgctgac tccttcactg
    16141 atctgcctct ggattcaaga ggaatctcca ctgggaacta tcaagttctt cgagaaaatc
    16201 ttcaaccttc tattttaatc gagatgggtt atatgaataa tgatagtgat ttaaaggaat
    16261 tagtaacaga cagttatcag cagcagattg ctgcaagttt gacacaagca ttgaccactt
    16321 attttcagta ataaataagc ccagccaaac aaaaatagct cctcatattt ttcgtttggc
    16381 cgggcttatt atcgcagatg caagctttta aacaccgttt aaggaagagg tcgtgagcct
    16441 gccgttcaac tctgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgatgac
    16501 tattgtgact tgtgaccgag gagttggcat gagaacaccg tttaagaaaa aggttgtgaa
    16561 atcaacgttt tgttctgagt attagagaaa caaacggaaa aatagttcct catattttgc
    16621 cgtttgttga gctaataccg aggatcagat gattgaacac cgtttattcg gaacaaggga
    16681 ctgtgacatg acttttgtca cagttccatt atttttttag tagcttcaaa gctttttctg
    16741 tattggcaaa atgaagtttg gcatattttt caagcatagg catgccgcct ttctccaata
    16801 gttttgccgc tacttggtca gatttcgttc cagctcctga cggaagggtg attccagctt
    16861 cagctgatgc tttgtctaac tcttccaaaa agctggcacg actgatgata gaagcagcag
    16921 caacagctaa atgatattgt tctcctttag taacgaagta cagtttttct tgtaattgat
    16981 ttttttctaa acgcgcatat tttttaaaat ttgcttccgg cgtaaactga tcgatcaaaa
    17041 tcgcttctgg tttttctggc tcgatttttt ttagcagcaa atggatcgct tgattgtgca
    17101 aagcgacttt catccgaacg gcattgtatt ttggttggat ctcattgtat ttttctggag
    17161 ataagacaag cagtttatag gggatggttt tttttaagat gtcagcaagc tggatgattt
    17221 ggctatcttt taactctttt gaatctctaa caccaagttt acgtaattct gcaagctgat
    17281 cacgtttcac ataagcagca catactgtta gcggaccgaa atagctgcca tttccgacct
    17341 catcactacc taaaacggat aattgactga tattttttgg caaagaagag gctgtttttg
    17401 ctggcttggc ttgtttatcc tgtgcctccc cccatttatc agcttctatt gctgcattcg
    17461 taccttgaaa catcactttt ccggatgtat aagcggtaat ggtcgtcccg tctttttttg
    17521 cagcaaagct agtataagga atctgtttat ttaacaaata agagcgatag tatgctttca
    17581 tttgactaag ttggtcgttt gttgctttga taatgacgtt gctcataaaa ttctcctcgt
    17641 ttttatacat aaaactatac cgcaactcac gacttttcgt tttctaagag attgcgagac
    17701 atagcatttt tttaaagttt atcatataat agttaagtat gtgaaaaata tttgtggaga
    17761 acggtgaaag aaccaacttc atggtacact aaataaaact ttcatcaaat aggaggaaat
    17821 gattcgtatg gcgcacgaaa aaacacgcta caaagcggtg attgccaacc aaacatatac
    17881 tattatcgga cgggaaacca agcatcatat ggatatcgta acgaagctga tcaatgaaca
    17941 gttagcagaa ttgaaacaat tatcaccaca aatggataat gaacaagcag caatcttgat
    18001 ggctgtcaat gcgctttcgg atcaattgaa gaaacaagaa agaatattgg agctggaaga
    18061 agaaacagca gaattaaaga aaaaaatgat caaatttaca gagcttgaaa atcgtgtgaa
    18121 acgcattgaa gcaatcgaga acgaagctcg ggaagtattg aaagaaaatg gtcaagcaga
    18181 tcataccatt aaaaatcatg tggaagcaca acagatttta aacgagaaac gaaaaggaca
    18241 aattaaacaa aaatcttcta attaagatcc ttttatcaaa ataagaaaaa tttaaaaaag
    18301 ttagaagaga aaggaacgta gcatgctgag tttgctgatt ttatttttat tgctaattgc
    18361 gtttttttct ggagcacgtc gcgggtttgc tttgcaaatc atattcgcga ttggatatgt
    18421 attgtcattt attgctgccc agcattttta caaaccatta gcaagtcatt tggaattata
    18481 cattccatac ccagctgtca ccccaacatc aaagttagca ttttttgatc aggtatttgc
    18541 ctttcaccta gatgaagctt tttatgcagg aactgctttt ctgattattt tgctaattgg
    18601 ctggctgctt acccgatttg tgggggtttt tgtccatgga ttgacgtatg tgccgatctt
    18661 gagacaggta gactggattg caggaggcat cctgtcacta atcatggctt acgttactat
    18721 atttttgatt ttgcagttac ttgcctttgt tccattagat gtggtccaaa atcaattcgg
    18781 cgagcatagt ttcgctcgtt ttatcgttga gagaacgcca tttcttacca acaagatgta
    18841 tgagctttgg gtaacgaata ttttgaatta aggaaaatag tgctggaaaa tatttcgttt
    18901 tttgtacaga gtagtttatt taagaaggct gcgtagcgta tgtccggcac agctcttttt
    18961 tcatagtgaa agataaggtg ataaaatgaa taaccgaatc cttgaaacat tggaatttga
    19021 aaaagtcaaa cagatggtca gacaatttgt agtgactgca caaggaaaag aagaactggc
    19081 agaattggta ccagtgagtg agaaacaaac gatcacaaac tggcttcaag aaactgagga
    19141 tggattgaaa gtgcaacgtc tgcgaggcgg gatccctata cctaaaattg aaaatatccg
    19201 tccccatatg aaacggattg agatcggagc agacttgaat ggagtggagc ttgcacaagt
    19261 ttccagagta ttgtctacga caagcgaatt gaatcgtttc attgatgatc tttctgacag
    19321 tgaaatcgaa tttgcacgtt tgtatatgtg ggcagatcag ttagtcacga tccctgtttt
    19381 gagccgccgt ttaaaggaag caatcgatga agacggacgc gtgactgatg acgcttctcc
    19441 tgagttaaag agcataagac agaacattcg gcgaagtgaa caagctgttc gtgaacagtt
    19501 agatgggatc gtccgtggga aaaatgcaaa gtatttaagt gatgcgatca tcaccatgcg
    19561 aaatgaccgc tacgttatcc cagtcaaaca agaataccgc ggtgtatttg gcggtgtagt
    19621 ccacgatcaa agtgcttcag gtcagacttt atttattgaa ccaaaacaag tggtggattt
    19681 gaataatcgt ttgcgtcaat accaaatcgc tgaacgaaat gagattcaac gtattttaag
    19741 tgaactttct gctgaacttg ttccccatcg tcaagaaatc atccacaata cttatgtgat
    19801 tggtaaaatg gatctgatga atgcaaaagc acgatttggt aaagaagtaa aagccatcgt
    19861 gccgggaatc agtgaagaca atcatgtcgt cttgaaacaa gcaagacatc cactgatcga
    19921 tcaggaaaaa gtagtttcta atgacatcac aatcggaaaa gactatcaag ccatcgtcat
    19981 tacaggacct aacacaggtg ggaaaacgat cacgttgaag actttaggtc tgttacaact
    20041 gatggggcaa gcaggattac cgattcctgc aggtgaagaa agtcaaatcg gtatttttga
    20101 ggaagtattt gcggatatag gagacgaaca gtcgattgag caaagtctat cgaccttttc
    20161 ttctcatatg acgaacactg tagatatttt atcaaaagtc aatgaaaaaa gtctggtatt
    20221 atttgacgag ctcggtgcag gaacagatcc gcaagaaggt gctgctctag caatcgcaat
    20281 cttagatgat cttggtaaaa agtctgctta tgtgatggca acaacgcatt atcctgaatt
    20341 gaaagtttat ggatataatc gggcaaatac gattaatgcc agcatggagt ttgatgtgga
    20401 tacattaagt ccaacgtatc gtttgctgat tggcgttcct ggacgaagca atgcgtttga
    20461 aatctctagc cgattaggtc ttgatacaga agttattgat gaagcgaagc agttgatgaa
    20521 tgatgagagc caagatttaa acgaaatgat cacagattta gagaatcgcc ggaaaatggc
    20581 agagaccgaa tatctggaaa tgcgtcattt tgtttctgag gcacaagaac ttcacgatga
    20641 tttgaaagaa gcttacagct atttctttga agaacgtgaa aaagaaatgg aaaaagcaaa
    20701 gaaaaaagca aatgaagtcg tgtcagaggc ggaagaaaaa gcagaaaaaa tcattgctga
    20761 tattcgtaaa atgcaacagc agattgggca aggaaatgtg aaagagcatc agttgatcga
    20821 tgcaaagacc caattagcaa atctacatca agaagaaact ttgaagaaaa ataaagtatt
    20881 gaagaaggcg aaagaacaaa aaacgttgaa accgggagac gaagtgttgg ttacgactta
    20941 cggccaaaga gggacgcttt tacgtaaaaa cgggaatcag tggcaagtag aaatcggtat
    21001 attaaaaatg aatgtgtccg aagacgagct gacgcctgtt gctcctcaaa aagagccgac
    21061 acaacgagtt atccatgctg tacgatctga atctagtagt cacgtgccga atcaattgga
    21121 ccttcgagga aaacgatacg aagaagcgct aagtgaggta gatcaatatt tggattctgc
    21181 cattctagct ggttatcctc aagtgactat tgtccacgga aaaggaacag gcgcacttcg
    21241 caagggaatt actgattatc ttaaaaacca tcggagcgtc aaaagctttg agtttgcacc
    21301 ggcaaaccaa ggcggaaatg gtgcgacaat tgttaaattt aagtaaagct tactaaaggt
    21361 aagcagatgg ttcgctatcc aagtatagaa atgctatact agtcatagat tttaaaaatg
    21421 gaggtctttg atatgtcaca agtaattaca gataagacat ttaatgaaga aacagataaa
    21481 ggacttgtat tgattgattt ttgggcaact tggtgtggtc catgtcgtat gcaagcaccg
    21541 attttggatc agttggaaca agaatatgat gaagaagaat tcagaattgc taaaatggat
    21601 gtggatgaaa atccagaaac tcctcaacaa ttcggtatca tgagcattcc aacattaatg
    21661 ttgaaaaaag atggtcaagt agtagaaaaa gctgttggtg ttcattcaaa agaacaacta
    21721 cgtcaaatga tcgatcaata tttataaaaa cttacagcaa aaataagctg agggtgggac
    21781 tgaatccagt tccgtcctcg gtttttttgc ggttgagtta aggttattca aattaatatt
    21841 ttttgagtat aattacgttg tcaggctgga ttgttttttt gacagacatg ctataataac
    21901 tttaattgaa tatgaagggg agtaccagat gaacgaacga atcaaaaaca aattagcttt
    21961 acttcctgat cagcccggct gttacttgat gaaagataaa aatggaacga tcatttatgt
    22021 cggcaaagcc aaaatattaa aaaatcgtgt ccgctcatat tttactggta gccataatac
    22081 gaaaacggaa cggcttgtta gtgaaatcga agactttgaa tacatcgtta cagaatcgaa
    22141 tatcgaagca ttgctcttag agatcaatct gataaagaaa aatgatccta aatacaacat
    22201 tatgttaaag gatgataaaa cctatccttt ccttaagata acgaatgaaa aatatccccg
    22261 tttggtgatt actcgtaagg tactaaaaga caaagcactg tattttggac catatcccga
    22321 tgtcggtgcg gcgaatgaga caaagaagat cctcgatcga ttatttcctc tgagaaaatg
    22381 caaacctagt cagacgaagg agccttgtct ttactatcat ttgggacaat gcctgtgtcc
    22441 ttattatttc gacgtagatc cgaaagtcta tacgaaaatc gttgatgaag tgaaaaactt
    22501 tttaaacgga ggacacgaaa aaatcgagtc agagatccat cagaaaatgg aaaaagctgc
    22561 tgaaaacatg gagtttgaaa aagctgcaga ataccgagac cagatccgtg ccattgagac
    22621 aatcatgact cgccaaaaaa tgacaaatac tgatttactg gatcgagatg tatttggtta
    22681 tgcgattgat aaaggatgga tgtgtgtcca agtgtttttc gtaagacaag gaaagctaat
    22741 tgaacgagat gtttcaatgt ttcctttcta taacgaagca gaagaagatt tcttgactta
    22801 cctcggtcag ttttatcagg aaaatgagca tttcattcct aaagaagtct tgattcctga
    22861 tacgatcgac aagcccagtg tagaagcttt gttagctacg aaagtgctgc aaccaaaacg
    22921 aggagaaaag aaaaaattag ttgaattggc gagtaagaat gcctcagttg ctttgaatga
    22981 acggtttgat ctgattgcca gaaaacagga acggacgatc ggagcagttg aacgtttagg
    23041 aaacgcgatg aatatcccga ccccaatacg tatcgagtct tttgataact ctaatatcat
    23101 ggggacgaat cctgtggctg cgatggtcgt attcattgat ggaaaaccgg caagaaaaga
    23161 atatcggaaa tataaaataa aaacagtcaa cggaccggat gattatgcat caatgagaga
    23221 agtcatctat cgcagatatt cgcgagtgat tcgcgaaggg ttgcctttac ctgatttgat
    23281 cttgatcgat ggaggaaaag gacaagtaga tgtggcaaaa gatgtcttag aaaatcaatt
    23341 aggaatcgat attcctattg ctggcatggt aaaaaacgat aaacataaaa caaatgagtt
    23401 gctattcggt acagatttgg ctgtagtacc gcttgagcga aattcaccag agtttttctt
    23461 actccagcga atccaagatg aagtccatcg attcgcaatc actttccatc ggcagctacg
    23521 cagtaaaaac agttttgctt cccgattaga tgaaattgaa gggttaggac caaaacgaaa
    23581 aaaaatgctg cttaaagagt tcaaatcgtt gaaaaatatc acagcagcca cgacagaaga
    23641 attacaagaa atcggactgc cgaaaaatgt cgcacaaaac gtatttgata aattacatca
    23701 ggaatgagga aagggcactt tacaaagaaa gtaaagtgtc ctatcatttt atgtacaaca
    23761 aaaagagcgt ttacgttttg ttggtaaata ataaatcgct tacgtctggt gtctaaggat
    23821 atatgtgatt tcttgaagaa ggagtgaaga aatgttagaa gtcaaggatc ttgttaaaac
    23881 ttttgggaac tacacagctg tcgatcatgt ctctttccaa atccccgatg gaaaaatcat
    23941 ggggctaatt ggccaaaacg gggctgggaa gacaacgact tttcgtttga tcttggattt
    24001 tttgacacaa gaccaaggag aagtgttatg gaatggccat catcttgggg agaaagatta
    24061 tgatgtcatc ggttatcttc cggaggaacg gggactttat ccgaaagtaa cgattcaaga
    24121 ccaactgctt tattttgcag aacttagagg gaaatcaaga aaagagattg aaccaaaaat
    24181 cgacttttgg atggaaaaat ttcaggtaaa agggaaaaag acggataaag taaaatcttt
    24241 gtctaaagga aatcagcaaa aagtccagct gatcgccacg ttaatccatg aacctaaatt
    24301 gatcattttg gatgagccat tcagcgggtt agatccggta aatgctgaat tgctaaaaga
    24361 tggaatattg gaattgaaaa aacatggttc atgtgtcatt ttttctagcc acaatatgga
    24421 taatgtagaa aaaatctgcg atcatttgat catgttgcga aacggaaaaa tggttttgaa
    24481 tggaaaagta catgagattc gtgaatcatt tggccgtacg aagttattct tggaatctgg
    24541 tttaagccaa caggaagtag aagaaatcga aggcgtgaaa aaagtgatcc ttcatgaaga
    24601 caatacgtta gagattacct tagaagatcc tgaagctggg aaagaaatct ttactcgtgc
    24661 gactcagttc ggctacattc ctatgtttaa ccagcaaccg ccaactttgg aagagatttt
    24721 caaattgaaa gcaggtgaaa tcgatgcgta agttttggat tattaccaaa gatgtctatt
    24781 taaaaaatgt taagtcgatt tctttcttga tcatgatatt agtccctttt atcatgctgg
    24841 gtatcattta tgtagcagga aactttgctc aacaaaatag tgatgtcaat aaaatcggcg
    24901 tattggctga taatgagcaa atcagtcagc aaatgagcca agtcaaaaca gatgattttg
    24961 agttcaaagc agttgattct gaagaaaaag cacaaaaaga gctttcggaa gaaaaaatcg
    25021 atgcgtattt agtagtctca actgagaatg ataagataaa tgggatgctt tatagcgaaa
    25081 attcattagg acaaagtaca caactactga tacaacaaca actgactggc ttgcaatcga
    25141 tgcttcgagc gagtcaatta ggcatttcac cagaagaagt agctagcttg agccaacaag
    25201 caagtttgac tcgacaaaaa gtcaattttg attcaaaagg aaaaatgaca gtcggagaag
    25261 ataactcgac agtccaatac gtcgtaagtt atgtagcaac gattgtcttg ttcattatta
    25321 ttttgacgta tgcgcagatc attgcacaag agatcgcttc tgaaaaaggc acgcggatca
    25381 tggaagtgat cctgtccagt acgactgcac aaaagcattt ttacggaaaa ttaacaggtg
    25441 ttttacttgt tgcattaacg caaatggcat tatacggtat tatttttggt ataggataca
    25501 gtcaattcaa agatatggaa atggtcaaaa atatattaga cggtatctca ttggataact
    25561 tgattggacc attcttgtgg tattctcttc tattcatgat ctttggcatt ttgatttttt
    25621 ctgttttagc agctttatgt ggctctttgg tcaacaaagc agaagataca gcaaaagcaa
    25681 ttttgccagt gacttatcta tctctaggcg gatacatgct tggattgata ctaggagctt
    25741 cagatccaaa caatatcgtt attcggatca cttcgtatat tccattctta tcttcatata
    25801 ttatgccgat tcgcttagca aatgagacag tcggtatggg tggagcattg atttccttgt
    25861 tggtacttgt agccgtaaca ttcttattga tgatcttgtc tgcaaacatg tacaaatcga
    25921 atgtactcgt ttatagcgaa ggcggattat ggagttcatt gaaacaatcg atttcaatta
    25981 tgaggaatga acgaaaaaaa gcataaaaca atcctaaaat acagaaggaa gctggacatc
    26041 tattgttccc agcttctttt tgtatagacc tattttattt attttactat attggatata
    26101 tattcattga aaatagtgta taacaacttt ataatattga gtataccaat ttttggaggc
    26161 gttttaatgg accgttattt agatttactt tcagaaaaat tcccctcaac ggaggatgtc
    26221 atcacggaaa tcatcaatct cgaagcaatt ttgcagcttc caaaaggaac agagttattt
    26281 ttgagtgata tacatggcga atttcctgct tttgatcata ttctgcgtat cggttcagga
    26341 aatcttaaag aaaagatcaa agaccttttt tctgaacaaa tgacggaaac ggatatcagc
    26401 caattcacta tttttacggc ttatccagaa tatgcattaa cgactgattg gtacaaagaa
    26461 caagataaat cgcagctgat ccatcatttg atcgatcttt tacggtttac gacagtcaaa
    26521 tatacccggt caaaagttcg aaaaggattg ccgaaagaat atagctatat cattgaggaa
    26581 ctcttgtata tcgatgaccg tatcaatgga aaaaaagact atgtaaaaaa aatcattgaa
    26641 caacttgttt tgatgaagga agaagaaaaa tttttaacga aacttgcaca gacgatccaa
    26701 aaatctgtca tcgatcattt gcatatcgtt ggagatattt ttgaccgagg aactcaagca
    26761 gccaaagtga tggatcgaat catggatttt tcttctgtag atatccaatg gggaaatcat
    26821 gatattttgt ggattggtgc gtattgcggc tctgaggcat gtttactgac acttttacgg
    26881 attgcagcac gctataatta tttatttgaa ttagagaaag aatatgggtt gaatttacgt
    26941 ccattgtttt cttttgctca tcaaacttat cagaagaatc cagtatttgc gcctaaaaac
    27001 agtaaaaatc tatccgaaag agagcaagaa gaattagaaa aaatccatca ggcactttct
    27061 atccttcaat tcaaatcaga acatcagtta ttggatcgtc gacctgaatt taaaatggac
    27121 gatcgtaaat tgctggaaaa aatcgactat gaagcatcaa cgattgattt agaaggtcag
    27181 ctctatgagt tgaaaggaac ttgttttcaa acgatccagc cagatgatcc aaaaaaattc
    27241 ttggaagagg aacaaaaagt gattgatagt ttgatgtatt cattccagca ctctttgaga
    27301 atgcaaaaac atatgacgtt tttgatcgaa aaagggggaa tgtatctcac gaacaatcag
    27361 catgtgctgt ttcatggctg tatacctgta gacgagaagg gacagctctt agcatttgaa
    27421 ttagaccaat cttatcgcgg aaaagaactg ctgggctttt ttgaaaaaca gataactgaa
    27481 agcgcaaaag atctgactgc aaaagaagat ttagcaaccg atttgatttg gtatgcgtgg
    27541 agtgggccat tttcgccttt attcggcaag agtaaaatgg cgacttttga acgttatttt
    27601 ctcacagagc ctcatacgca cgtcgagaaa agcaatcctt attatcactt gcgagatgaa
    27661 gagtggttca gtgaaaaaat tttagcagaa ttcgacacaa aagaagaagg gtcagcaatc
    27721 attaatgggc acacgccagt aaaagtaaaa aaaggggagt caccgatcaa agcaaatgga
    27781 aaagtatttg tcattgacgg aggtttatcc aaagcttatc aaaaaacgac aggaattgcc
    27841 ggttattcac tattatgcaa ttcttatggt ttccaaatcg tgacacatta tccgtttttg
    27901 cctattgaac aacaatttgc aaaaaaatcc gaccaaacaa atgtgaaaaa agtaatcgaa
    27961 cagcaactac cacgcaaact caatcgtgat acaacaaaag gtatggaact gcagcagcag
    28021 attacccagc tcaaaaggct gttggactat cgaaaggacg attgagaaga attccagtga
    28081 acattgttcc atagactgga gactgagaca aaagtcgtgt cgcagtctct ttttccggat
    28141 aaacggtgtt cttctgccaa ctcctcggtc acaagtcaca ctattcaaca aacatgaaaa
    28201 gctgtttttt gaaattgctc cttgtgactc agagttgaac ggcaggctca cgacctcttg
    28261 gtataaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgtg
    28321 aaaagccatt ttcacaaaat ttttcttaat actcgaaact aaacagcttt ttcacaaccc
    28381 cctcagaaag tctattaaaa ttcattcaca aagaaatcga ataagcgctg ttcagatgga
    28441 tgtacacggt gggtaagttc cggatgccat tgaacaccga ggattttatg catatcatct
    28501 ttgctttgaa ccgcttcaac gagtccatcg ttagaaaaag cgatggcttt taataaaggt
    28561 gaaagatctt tgactgcctg atgatggtaa gaattcacaa aatatggatc ggggagaagg
    28621 ttgcttaaca aactgtctga aacaatcttg acttcgtgag tcgtaaattg cggggcagtg
    28681 ggctgttgtt catgttttac tgaccacttt ggatataatg aaagatcttg ataaagtgta
    28741 ccttccaaaa cgacattcaa tagttggagt ccccggcaga ctgcaaaaat tggttttctt
    28801 tgtttcaatg cttccaagat cagtgcctgt tcaaattggt ctcgttgaat atttgtttcc
    28861 gttagctttg gatgaggatc ttccatgtaa agttgtggac tgacatcttg accacctgca
    28921 agaataagtt tgtcgatttg tccaatcagt tgaggagctg aatctggaga catgactggc
    28981 agaataagag gcaatccttg agcatctaaa actgcatcca cgaatccttg cggtgtatag
    29041 ctcacttgat ttccttggaa agtatctgtt gctcgaatca gttgatttcc ggcaatgccg
    29101 atgattggtt tcatttgtat tccccctaaa gtagacttat tgtaacaagg atttaaaaaa
    29161 aattaaacta tgtaagactt gcccctatac ttatctgttc ttgctgtgtt actatctgta
    29221 aagagaaatt tcgtgtgaaa ttcatgacga tagttgaaaa tattgcttaa tttgttataa
    29281 taaaaagtaa gcagaaataa aaaggttggg cgtaagcttc aaggatctag ttcttcaagg
    29341 ggtgagaagg acattttatt gaaggggcct gcctatacta agttatcttt agaggtacat
    29401 actttcaatg aaaaaagaaa gcatgtacct cttttttaaa tcgaaaggaa gtgaagcaag
    29461 tgttagaagc tttggaacaa atccgaaaag cagaagaaga aaatgaaaag cgtgctcaaa
    29521 cactgcggca gcagctgaag atctatgcaa cagaaaaaga gcaggaattg gaaacgatcc
    29581 aaacaaacta tcgacaacaa ctaaaacagt tggtggaaga aaaagaacaa tttgaaatac
    29641 aagaattaga aaaagaaaaa atgaaattga aagcagaagc agacaaggtg caagcaactt
    29701 tgaaagatca atatcaaaag catcagagtc aagcgatcgg tgcgatcatt gaaagggtga
    29761 aagaaactta tggccgtcac taagatggaa aaagtgaccc tcatttcaga taaaagaaat
    29821 caggagagga ttttacaagc agtacaagga atccaaaacg ttgaaatccg ggatctgtat
    29881 caaaatagaa caaacaacca gtgggtggag aagtattttc cagataccac gattattgat
    29941 aaagaagcaa aattaagcgc tttaggtaat cggttgaaag acatccaaga agctatccaa
    30001 ttcatacaac accacggcga tagccgtcaa aagaaactcc atttgaaacg tcaagaactt
    30061 tccttacaag atttggaagc caattattcc gaagaaaatt tcataaataa attgaaagaa
    30121 gtccttgatt taaaagaaaa gtgggaacaa ctaactgaag aacgtgatcg tttagaagaa
    30181 gaggaagaat ggcttcttca ttggcaacag ctggatattg ctccaagtat gtatgcaagt
    30241 aagacgacca tttttgagat ggggactgtg agtgcagcaa attttgaaag tttcaaggaa
    30301 gaactgagta agcttgatga cgtttatatg gaagaaatag attatacgcc aaaacaagtt
    30361 tatctttcgt atgttgcttt aaaaaaatca gttgctgtta tagaagaaat tgctgggaaa
    30421 tacgggttca ataaagaaac gtatccttat gaacaaaatc ctaaagaaaa actgcaagag
    30481 accaagcagc aattatccat tgttcacgag cagcaaaaga agataacgac agcgttaggt
    30541 cagtgcagtg gctatatcca agaatttgaa tgggtagaag aagtcacact tgccgttgca
    30601 gaacgagaaa aaataaaaga acggtttatc catgcgactt atctgatcgt tttgcaaggt
    30661 tgggtagatg cagaagaaaa acagaatttg ctgcatgtgt taaacgaaac cgtatcagaa
    30721 gaagatatat acgtcagttt cgaaacacca aactcagaag agattcagca ggaagtccct
    30781 actaaattga aaaaccatcc gcttgtcgaa ccttttgaat tgctgactga aatgtacagt
    30841 ctgccaaaat atgaagaagt agatccaaca ccttggatga caccatttta tcttgtcttc
    30901 ttcgggatga tggtcgcaga tgttggttat ggtttgctga tgctcattgg gacaatacta
    30961 gctcaaaaac tacttgttct accacgcggg atgaaacgtt tcgtaaaatt ctttgagatt
    31021 ctctcgatac catcgatcat ctggggattg atctacagtt catttttcgg acagtcatta
    31081 ccaaaagaac tgtttggcat ccgcttgcct ttcccaatcc tatctacgac ggatgacgtc
    31141 aatacgattt tgattttatc cgtgattttc ggtctgatac agattttggt tggattattt
    31201 gtggcagcaa aggaaaatat caggagaaaa gattacttga gtgctgtcag tgatggattt
    31261 gcctggcaag ggatcctaat tgggatcgtt attgcgttgg ttggtgccat gttgataaaa
    31321 aatacagcat ttgtttacct tggcgctggg ctatcgatca ttgctgcctt atgtatcttg
    31381 atcgttccga ttatccaaac gccgtctaaa gtcaaaggtg ctgccaaagg tgcgtataat
    31441 ctttacggac tgaccggcta tatcggtgat ttagtcagtt atacacgttt gatggcattg
    31501 ggtatttcag gcggaagtat tggagcggca ttcaatatgc tagttgcttt catgccgcca
    31561 gctgctcgat tcagtgtagg gatccttttg atcatcgctt tacacgcact gaatatgttt
    31621 ttgacactgc taagtgccta tgtccatggc gcacgattgc agtatgtcga attttttggg
    31681 aagttttata caggaggcgg acgtgccttt gatccgctga aaacggcgga aaaatacgtc
    31741 aacatcaatc acaagaaaaa aaacaataaa taattggagg atttttatat aatgatggat
    31801 tacttaatta gtcaaaatgg tggaatggta tttgcggtat tagcgatggc aacagcgacg
    31861 attttttcag ggatcggttc agctaaaggt gttggaatga caggtgaggc agcagcagca
    31921 ttgacaacaa gtcaacccga aaaattcggg caggctctga ttctgcaatt gcttccaggc
    31981 acgcaaggtt tgtatggatt cgttattgcc tttttgattt ttatcaactt aggaaatgac
    32041 atgtcagttg tacaaggact gaactttcta ggtgcttctc tgccgattgc ttttacaggc
    32101 ttgttttcag gtattgccca aggaaaagtc gcagcggcag gtattcaaat tttagcgaaa
    32161 aaacctgaac atgctacaaa aggaatcatt tttgctgcga tggttgaaac atacgcgatt
    32221 ttaggtttcg ttatttcctt cctattagtg ttgaatgcct aagcataaag gaggaaaagc
    32281 agatgaacgc cattgaaaaa atcatctctc agatgaacga agcagcagag caggaaagag
    32341 cggcgttaga acaagaagag cgaatgaaga tcgatcaaaa ctttgaacaa aaacgtacgc
    32401 aagttgaaac agaacatcaa aaacaaaaag aaaaacaaat cgaattactc gaaaaaaaat
    32461 accgtcagct aaggaatcgc cagcaagtag aagtacgaca agaaaatttg aatgcgaagc
    32521 aggaattttt aagacgattg tttgcagatg cggtaactga aatggaaaat tgggatgaat
    32581 ctgaacaaat ccagtttatc aaaaatgcat tgtattcatt acctttgaca ggaaaagtag
    32641 cattcattgc tggagaaaaa tcagcagcct atctttctca gacgttgttg gatgaatgga
    32701 acaatgaact tccctttatg atggttttaa gtgatgaaac tgttgctgat caagcgggtt
    32761 ttctgataaa cgatcaagga gttcagtata attttctttt cagcagcctt gtacaagata
    32821 tccaaggaac gatgagtttt gagattgcca atcaattatt cgaataagga gggatcagtg
    32881 tatggaatac cacgaactaa atcccttgat tcgcggaaga gagcttgaac ttttgtccaa
    32941 tgagacattt gaacggatga ttcaaacaaa ttctgtcgaa gcacttggtg aaatattgaa
    33001 atccacgatt tacagtccat atatttatga cggatatgaa tatgacttcg aacagaatct
    33061 tttaaaggag cagagccgtc tatttcgctg gttaaaggaa tcagcaccgg agcctgaaat
    33121 cgtttggatc tatacgatgc gttatacttt ccataatctg aaggttttga caaaagctga
    33181 gatgacaaat caaaatctgg accatttgta tatcgacgat ggattttatt cattagaaac
    33241 cttgaaggat gccattcata cgcaagtttc aaatgaactt cctgaaagca ttatggacag
    33301 tatacgagaa gtacgtgatt attttgaaga gtctaccatt cttcagggaa ttgacgtgat
    33361 ttatgatcga cagtttttga cagagcagcg acggcttgcc gataagctag gatatcaaga
    33421 attgctgaca gaagtcattg cttttattga cttgaccaat atcacaacga tggctcgagg
    33481 aatattgcag aaaagaacat atgggttcat gacgacggta ctttccagct cgggaagtat
    33541 cgaaaaagaa gtatttttga actttgtaga taaagatatg gaagattaca cgaattttct
    33601 attagagaca gattatgctg attttctgcg ccctgtcatc cgtgagggaa atattgatct
    33661 cgtaaagctt gaacagttga aagatgatta tctaagttca ttgtatcaag aagcacaaac
    33721 ccaggctttc ggaccactgc cactactagc ctttttgaac gcaaaagagg tagaaagcaa
    33781 aaaccttcgt ttactggtta tcggcaaaag aagccatttc gaatcagaaa caatcagaaa
    33841 gcgggtgaga caagtctatg acgcataaga tcggtgtagt tggcgacaaa gattctgttt
    33901 tgccattcaa attgtttggc tttgatgttc gttatggtac aaccaagcaa accgttcgtc
    33961 aggcaattga agaaatggca aagaatgagt atggcgtgat ttatgtcaca gagcaatgtg
    34021 cagcattgag cccagaaacg atcgaacgtt acaaggaaca aatgatacct gctatcgtat
    34081 tgattcccaa tcatcaagga tcattaggga tagggctgga agaaattcaa aaaaacgtag
    34141 aaaaagcagt aggtcaaaat atcttatagt atgaaaagga gatagaaatt ttgcaaattg
    34201 gaaaaatcat aaaagtctcc ggacctcttg taatggctga gaacatgtct gatgccagta
    34261 tccaagacat gtgtctagtg ggagatctag gagttatcgg agaaattatc gagatgcgtg
    34321 gcgatgtggc gtctattcaa gtatatgaag agacttctgg aattggtcca ggcgaaccgg
    34381 ttcgctctac tggtgaagct ttatctgttg aattaggacc agggatcatt tcacaaatgt
    34441 ttgacggaat ccaacggcca ttagatacct ttatggaaat cacacaaagt aatttcttag
    34501 ggcgcggagt tcaattgccc gcattggacc atgagaaaaa atggtggttc gaaccaacag
    34561 tagaagccgg agaaacggtg agtgctggag atgttatcgg gatcgtagaa gaaacaaaag
    34621 ttataaaaca taaaatcatg gtgccaaatg ggataaaagg aacgatcaaa acaatcgaaa
    34681 aaggttcatt cactattgat gaaacagtat gtgtcattga aaccgaaaat ggtgacaaaa
    34741 atttgacgat gcttcaaaaa tggccagtaa gacgtagtcg gccaatcaaa gaaaaattaa
    34801 acccggatgc acccatgatc actggacagc gagtgattga tacattcttc ccagtgacaa
    34861 aaggaggagc agcagctgta cctggtcctt ttggtgcagg gaaaacagtc gttcagcatc
    34921 aaattgcaaa atgggccgat gtggacatgg ttgtctatgt gggatgtggc gagcgtggaa
    34981 atgagatgac ggatgtttta aatgaattcc ctgaattgat tgaccctaat acaggagaat
    35041 cgttgatgga acgtacggta ttgattgcta atacatcaaa catgccagta gcagctcgag
    35101 aagcttctat ctatacggga atcacaattg cggaatattt ccgtgacatg ggttatgacg
    35161 ttgctatcat ggcagactcc acttcacgat gggcagaagc acttcgtgag atgagcggac
    35221 gtttggaaga aatgcctggt gatgaaggtt atccagctta tcttggttca cggcttgcgg
    35281 aatactatga acgatctgga agagtcattg cattaggatc agaaggtcgc gaaggaagta
    35341 taaccgcaat cagtgctgta tccccgtctg gaggggatat ttcggaacca gtcacacaaa
    35401 atacattgcg ggtcgtaaaa gtattctggg gtcttgattc cagtttagcc caaaaaagac
    35461 attttccttc gatcgattgg atacaaagct attctcttta ttcaacagaa gtagggaagt
    35521 atatggatca tatccttcaa gctgactggg catccatggt caccgagggc atgcgtattt
    35581 tgcaggaaga acagcagctg aatgaaatcg tgcgtttggt agggattgat tccctttcag
    35641 ataatgaccg tctgacatta gaagtggcaa aatccattcg tgaagattat ttgcagcaaa
    35701 atgcttttga tgatgtcgat acattcactt caagagagaa acaattcaaa atgttgaaga
    35761 tgatcttgac ctttggtgaa gaaggacgcc gtgcgttgtc gttaggtgct tatttaagtg
    35821 agatcatgga cggtaccgta tcgattcgtg aaaggatcgg acgaagcaaa tatatcccgg
    35881 aaacagaatt agaaaaacta gatcaaatta ttggtgaaat caagcaaacg atgcaaacga
    35941 tcgtttcaga aggaggcatg agcaatgatt aaagaatatc gtacgatcgg tgaagtcgtc
    36001 ggtccattga tggcggttga aaaagttgct ggtgtcaaat atgaagaatt aatcgaagtc
    36061 cgtatgcaaa atggcgagat tcgtcgtgga caagtattgg aagtccaaga agataaagca
    36121 atggtccaaa tattcgaagg aacaagtggt atcaatttac gtgactcttc tgtacgattt
    36181 ttaggccatc ctttagaact aggtgtatcg gaagacatga tcggtcgagt attcgatggt
    36241 ttgggacgac cgaaagataa cggaccagag atcctgcctg aaaagcattt ggatattaac
    36301 ggagaagtaa tcaatccagt agctcgggat tatccagatg agtttatcca aacaggtatc
    36361 tctgcaatcg atcatttgaa tacattagtt cgtgggcaaa aattaccagt attttctggc
    36421 tctggtcttc ctcataaaga attagctgct cagatcgcgc gacaagcaac ggtactcgat
    36481 tcatcagatg atttcgccgt agtctttgct gctattggaa tcacttttga agaagctgaa
    36541 tttttcatgg aagattttcg tcaaacgggc gcaatcgacc gttctgtcat gtttatgaat
    36601 ttagcaaacg accctgcaat cgaacgtatc gctactcctc gtatggcttt gactgctgca
    36661 gaatatcttg cttatgaaaa gggaatgcat gttttggtta tcatgacaga catgaccaac
    36721 tatgcagagg cattgcgaga gatttctgca gcacggcgag aagttccagg aagaagagga
    36781 tatcctgggt atctttacac gaacttagct actttattcg aacgagcagg aagaatccgc
    36841 ggactaaaag gatcggttac tcaaattccg attctaacaa tgccagaaga cgataaaact
    36901 catccaatcc ctgacttgac tggttatatt acagaaggac agatcatctt gactagagag
    36961 ctttacaaga gtgggattca gccaccaatt gatgtactcc cgtctctttc gcggttaaaa
    37021 gataagggaa ctggacccgg aaagacaaga gaagaccatg cagctacgat gaatcagctt
    37081 tttgcagcct atgcacaagg aaaacaggca aaagaattag ccgtagtttt aggtgaatct
    37141 gctttatctg atgtagacaa gatctatgcg aagtttgctg agcggtttga aaaagaatat
    37201 gtcaatcaag gattctacac gaaccgttcg attactgaaa cacttgatct agggtgggaa
    37261 ctgctatcga tgttgccaag aaccgaatta aaacgaatca aagacgacat gctggacaaa
    37321 tatctgccag aagggaagtg agttctgatg gcacgattga atgtgaatcc gacccgcatg
    37381 gaattgactc gattgaaaaa acagttgaca actgccacaa gagggcataa attattaaaa
    37441 gataagcaag atgaactgat gcgtcaattc atcctgttga ttcgaaagaa caaccagctg
    37501 cgtcaagaaa tggaagagtc tatgacagaa gcaatgtctg attttgtatt ggcaaatgct
    37561 tcgatcacgg aacctttcat cgaagaatta tttgttttgc cggcggaaaa cgttgatttg
    37621 tctatcgtag acaaaaatat catgagtgta aaagtacctg tgatgaattt tgaatatgat
    37681 gaacagctga cagacacacc tttggaatac gggtatttga attccagtgc cgaactggat
    37741 cggtcaatcg atcggtttac taccctgctt ccaaaacttt tggaactgac agaaatcgaa
    37801 aaaacttgtc aactgatggc gagtgaaatc gaaaagacac gtcggcgagt caatgcatta
    37861 gaatatatga cgatccctca attagaagaa acgatctatt atattcgtat gaagctggaa
    37921 gaaaacgagc gcgcagaagt aacaaggttg atcaaagtaa aaaatatggg taccactgac
    37981 taagtggtat ctttttttaa aagaggaaaa cgatgattaa acaaagcaaa caaataaaga
    38041 aacgtttgcc ggctgttcag cttctcgcat taggattttt tctcctgatt ttagttggcg
    38101 gcggcctact taccctacca ttttttagta atagtgggca tgggacaaat tttatcgatg
    38161 cgcttttcac tgcaacttct gctgtctgtg taacgggatt gacaacattg aatacagcag
    38221 agcattggaa cagtgctggt caatttttga tcatgctttt gattgagatc ggcggtcttg
    38281 gttttatgat ggttccgatt ctatgctttg cattgataaa gaagaaagtc agtctcagca
    38341 cacgtattat attaaaagaa gcgctgaatc tagaagaagt atcaggtgtg atgaagctca
    38401 tgctttacat cctaaaattg gcctttgtga tccaattgat tggtacggtt gctttatctt
    38461 ttgtatttgt gcctgaattt ggctggttga aaggtatctg gtacagtttg ttccatgcaa
    38521 tctctagttt ttgtaatgca gggtttgatt tgttaggaga cagtttagcc aatcaccagc
    38581 aaaatgttta tttgatcatg gtggtatctg ctttgatcat ttctggtggg ttaggcttca
    38641 tcgtatggcg tgatttactg gaatatcatc agaaaaagaa gatgacgatt cattcaaaaa
    38701 tcgcattaac cgttacaggt acccttttat taggcggatt cgttgtgttc tttttcacag
    38761 agcgcaatgg ggcaacgcta gtggaaggga atatgtttca acgattggca aacacctttt
    38821 ttatgagtgt gacacctaga acggctggat attattcagt tgattacctc catatgagcc
    38881 atgcaggtct gattttaacg atgtttttga tgtacatcgg cggaacatct gggtcgacgg
    38941 ccggcggatt gaaaacaact acgttaggtg tgttgctgat ccagatgcgt tcgatgttga
    39001 aaggacgtac gagagcggaa gcatttggcc gtaccatccg acaaggcgca gtgttgcggg
    39061 cacttacttt attttttgtt acgttgacgt tgtgtgtagt cgccatcatg attttatcgg
    39121 tgactgaaac gatacctaaa acttctggca tcgaatatat tgcttttgag gtcttctctg
    39181 cgtttggtac agtaggtctt acgatgggat tgacgccaga cttgactgca atagggaaat
    39241 tgatcattat agcgcttatg tacattggac gagttggtgt attgactgtg gtcttctctc
    39301 tgatgacaaa agcacaaaga acacaatcaa aatacaaata tccagaagaa agtgtcatga
    39361 ttggataatt tcatcttctg acagaaacct agtgatttag taaaataaga aaataaaata
    39421 atttttcttg cttcttggat actttaagtg caaaatgttc gtttattttt tgaggcacta
    39481 cccgttgttg aacctcgggt agtgcctctt ttttattgaa aggaatcaat aagatttgat
    39541 tcctttattt atcaataggt tataattcat atttttttat tttttctttt acgatactta
    39601 gtccagaagc taaaatttca aggttattag aggataggga aattcgaacg aatgcatctg
    39661 gttgcgcacg tgtagtaaag cgtctggaat gataaagtcg gataccgtta gcaagaaagt
    39721 cattctcaat tatcttaggg tcaatctcag gatcaactgg aagaattcga tagtaaggga
    39781 cacgattcgt tggtttttct actttgaaaa gagaatcaaa aagctgattg gctctttttg
    39841 taagacttaa tttagtttct aagatcctat ttgcttcatt tgaatggagg atttgagtga
    39901 tgacttccac atctaatcca gaagttttca catttatatt aaaaattgct tgttctatct
    39961 tgctttggac atgtactgga actacaagat aagcaactcg caatccagaa caaataaatt
    40021 ttgtcattcc agctaagtag attgtttggt taggtaataa attttggaaa ggaggaatga
    40081 tcgtatgttg atagtaagtt gttaaaaatg aatggatatc atcttcaata acccatagac
    40141 cttctttttc aatcacttcg gctaattgct ctcgtctttt gtgagagatt tgaaacccta
    40201 taggattatt acaagaaggc ataagaaaga caccatgaat cttctttttt ctacattcct
    40261 gaatcaatat atcaggtcgc attccttgct cgtcaaaatc aattggaacg atctctaaat
    40321 gatataactg agccaattca atgaaattag cgtatgtata tcggtctgtt gctattctat
    40381 cacctggaga gaaaagtgca gaaagtgtga tagccaatcc attttgaaca ccggaaacaa
    40441 tagatagaga ttctaaggac ggaatgactc cttgagtttt taaccaattt gctgcagtag
    40501 tcaattgaaa atttgtgcct ttgggatctt ggtaattaag caaaccaaca agatttgggt
    40561 tattactagt tgattgaata aacgggacaa cgaatttatt acattcttca aaagaactaa
    40621 ccagaccaaa atcaatgata tgttcggtta cttcattggt agagatggtg atggactcaa
    40681 agacattggg agaaataaat gtgccacttc caatattcgt atacagcaaa cctttctcaa
    40741 ttccatattt ataagccttg ccaacggtag taaaatttaa atctaaataa tcagctagct
    40801 ctctttggga gggtaactga gtgtttttct gtaatttatt ctttaaaata tcttgttcta
    40861 attgttgaat caacgatcga taataaggac gcttcaattt ttgtttgtct ggtcgccaag
    40921 ttaacgggta attgtcataa gaattaatag gcattttctc cactccttaa aattgtatac
    40981 catacaattt taccattgat tgtattgatt ttagaagtta atcttttgtg aggagggaat
    41041 tgtctatgaa aattattaat tttgaggaag tagtagcttc ttttagtttt gaagaagcga
    41101 ttcaagtaat gaaaaaatgt ttttttgatt atgaaagtgg taaaatcagc caaaatcctc
    41161 gattggtgga aattttacct gacggaaaag gcaaaaatat gtttgcgatg atgcctgctt
    41221 atttggggga gaacagatat tttggcacaa aagtcattac cgcttttccg gataatcatc
    41281 agtatcattt accttctcat ttaggagaga ttttcttgtt tgatgctcaa aacggactac
    41341 ccaaagcatt gataaacgca aatgcagtca cctggattcg aacagctgct gtatctgcgc
    41401 tggcaacaga ttacttagca aatccgcatg cagaaacact tgctttaatt ggagctggtc
    41461 aacaagcatt gtcgcatttg actgcgattt ctaccattcg tcccatcact acagtgtttg
    41521 tttacgatat tttagaagaa agacgaaata cttttatcgc taaagctaaa aaacagtatc
    41581 ctaaaataac gtttataaat gcaatatcag tagaagatgc agtacgtcca gcggaaatca
    41641 tttgtacgtt gacctctagt aaagaggcat ttcttcaaaa agagtgggta agggaagacg
    41701 cacatatcaa tgctgtgggc acatttactc ctgatacaag agagataagt acatcgttga
    41761 tggaaacttc tcaaatctat gtagatgatt actcagctgc cttaacagaa agcggggacc
    41821 tgcttatagc catggcagaa ggagatttag aggaatcttg tattgttggt acattaggcg
    41881 aattagtttc tgagaaaaaa acattttata aaggagaatc gaggccgacg attttcgacg
    41941 cagttggtct agctattgaa gatctttgtt gtgcagaata tatttataac aaattagaga
    42001 aaggggcttt aaaatgaact tagattatta cgtagttgat gcatttgcag atgaggtgtt
    42061 caaaggaaat ccagcagcgg tttatgtgct agaagaatgg ctgccagaag gtacgatgca
    42121 aaaaattgcc attgaaaaca atttatctga aacagcattt acagtgaaga aaaaccaaga
    42181 atttgagctt agatggttta cgccagatcg agaaatcgat ctttgtggtc acgcgacatt
    42241 agcaacagca tttgttttat ttaactatta caaaatacct gatgaaacga ttaaattttc
    42301 tactcaaagc ggtaatctat ttgtgacaag aattaaagat tattattaca tggattttcc
    42361 tagtattatg ccgaaaaaag tgccgatatt gtctgaatat gaagaagcga ttggggctaa
    42421 gattaaagaa gcctacttag cacgtgattt atttttcgta ttagaagatg aaaaaacggt
    42481 ggctaaattg cagccagatt ttacagctat caaaaatttt gaattaggaa taggagtcat
    42541 cgtgacagca gaaagtgtgc aaaaagactt tgtttctaga accttctttc caaaattaac
    42601 aatcaatgaa gatccagtat gtggatcagc acattctaat cttattcctt attgggcaga
    42661 aagaatgaat aaaacaaaat tgtcagctta tcaaatgtca tcaaggggcg gggatctaga
    42721 atgtgaatta aaagaagatc gtgtgataat aggaggaaaa gcagttttgt tttctaaagg
    42781 tacctcgttt atttgatttc tttcaaatga attaaaaata aattggtgga gagtggcaac
    42841 cttatagcta caatggttgt taaaaaaaga cgcaaacttt ttcaatttaa tgaaaagttt
    42901 gcgtctcatt ttaattattg gtgtttgttt tttattcaat caagagtaaa atgccccata
    42961 ctaactatcc tcggatgact tcgattttat aaccatcagg gtcaatgaca aagtagtaag
    43021 aaggcgcagt gccaggtaat ccttttaagt cagtaatctc aagaccagca gctttatgtt
    43081 gctcgtgtaa tttttctaga tcatcagctg ctatagcgat atgaccatag ccgtctccta
    43141 gatcatacgc accatgatca tagttgtaag tcaactctaa ttcatagtca tccccaggaa
    43201 gtgtcaaata aacaagtgta aatttgtttt ctggaaaatc acggcggcgg ctttcttcaa
    43261 aaccaaatgc cttttgataa aattcgattg atgcttcaag atctttcact cgaacacatg
    43321 tgtgagccat tttcatagat tcatctctcc ttttcctttt actcaatcat aacaaaaaga
    43381 agtaaggaaa tagttgattt gtgcttgttc atgagaaatt tctcatgtaa aatggataga
    43441 aaataaaact gttaaggtgg aaactattca tggataaacc aatttttggt gaaaaaaagc
    43501 aatttgctga ttatcaaacc cgttatgcag catatattgt catagaaaga gatcagcaga
    43561 ttgcagtaat cgaggctcca aatggtgctt tttttcttcc tggcggggaa attgaaggag
    43621 aagagacgaa agaagaagcg attgtccgtg agctgataga agaaatggga attgtggcag
    43681 aaatttcttc ttatctggga gaagctgatg agtattttta ctcgaattat cgtgcaactt
    43741 attattataa tccaggatat ttctttgttg ctgattcttg gaagcgtata ggagaaccaa
    43801 ctgaaaagac caataaaatt tggtgggtga gccctcaaga agcgcttgtg aaattgaaac
    43861 gtggtagcca ccgatgggca gtcgagagat ggatcgaaaa aaataaaaac tgaaagaaag
    43921 cgagtagatc agctttctct cagttttttt atttattaca gtacatattt tttgattgct
    43981 tcagctacac cgtgctcgtc gtttgatgaa gtgatgacat ttgctgcttc tttgacaaga
    44041 gaaacggcat ttcccattgc tactcctaga ccagcatatt caatcatgga taagtcgttc
    44101 tcgttatctc cgatagccat gatttcatct tgatcaatac caaggtgctg tgcgagattc
    44161 gcaactgccg cacctttact tgcttcttta tttagtactt cgaagtaata gggcgcactt
    44221 ttgaccgttg tgtatttatc acggaaagtt tgcggtaaac gcgcaattgc tgcatctaaa
    44281 atttctggtt cgtcgatcat catcattttg acgatgttca tgtctggtgt catttcttca
    44341 ggtgttcgat attttagcgg catcttgact aaacttgctt cgtggatcgt ataagcgctg
    44401 atatctcgat taggcgtgta gatcgctttg tcatcgatcg aatggagatg actgccgaca
    44461 cggcgtgcca ttacttcgat ctctaggaaa tcttcatgtg tcagtccgtg acgggcgatg
    44521 attttacctg atttcgtttg ttgtacgagt gccccgttat aagtaataac gtaatcatta
    44581 tcttgaaaaa ggttcaactc gtttaactgt tcctggacac ctggtaaggg acggccagtg
    44641 cataaaacga tcgaaacacc ttttgcaaca gcagcggcaa tcgcttcttt tacttctttt
    44701 gttacttctc ttttctcatt taataaagta ccatcgatgt cgatagcgac tagtttgata
    44761 gacataattt cctccaatat attttaattt atttcaatca atgcgccatt acggatatgg
    44821 ctggcaaatt cttgataagt ttcgtcaaat aaatcataat gatcacgcaa ggacggatca
    44881 accatttctt ttggaaagaa aaatcgctca tctccttgtt cttgtcccgc taatgcagca
    44941 acaagctgac taacctgtga taattctatc aaagttccgt catttcgcaa aatctcgatt
    45001 tgtgtgcgat gtctgccttg ttctgggcga taaaaatcat aaggtagatc gtagctagaa
    45061 ttcacagcag tgtaataaac aggattatat ccgacctttt ctactaacaa agtgagttct
    45121 tggactaatt ttgaatcccg gttgccagaa aaagtagcag atttcagtgg tttacgattc
    45181 aaaaaacgtt tagctaaatc atttaaaata ggatcagcag attcagacca ttgagtaaag
    45241 taagtgccca agaccccatc atcaagtttg aggtattctt ctaaagtaaa gcttccgtca
    45301 agaaatggca gaagtaattg agaatggagt tcaaaactgc tggcatcttc ttgatacaac
    45361 tcgtgtgcgc gatgaagcag atgctccaaa attacttcca taccacggga aacaggatgg
    45421 aagtacactt gcacatacat ttgatatcgg ctaacgatat aatcttccac tgcatgcatg
    45481 ccactcatgg aaaaagcgat cccgccttca taaggacgaa tcacacgtaa tattcgggta
    45541 agatcaaatg ttccatattc tgttccagtg aaataagcat cccgaagcaa atagtccatt
    45601 cgatctgcat cgatctgact agaaatcatt tggacaactt gtgggttggg gtaagttttt
    45661 tggatcacac tagctacctt ttcaggaaaa ccttcttcta ctcggttaag aatctgatac
    45721 acttctgttt ctggagaagt gatgatctct acagtgatgg cttcatgatt cgtatggaag
    45781 atatgttcaa atgtatgaga gtaaggacca tgaccgacat catgcaacag tgccgcacat
    45841 aaagtcacca gccgttcctg atcatcccag cctccgttgc ctattttttc ttttgaaaaa
    45901 tttcgagaaa agatatcgca gattcgtctt gaaatctcat aaacgcctaa tgaatgagta
    45961 aaacggctat gttctgctcc gtgaaaagtg aaagaagaag tgcctaattg tttaatgcgt
    46021 cgtagacgtt ggacttcttt cgaattgatc aaatctaaga ttacttgatg ctggacatgg
    46081 atatagttat ggactggatc acgaaaaact ttttccatag gcagcatttg atatttataa
    46141 ggggttgtca taatgtagct cctttatgtt attacgttgt tccattctct ggatttggtt
    46201 gttccattgc tccagctgga tttctttttt tagccagtcg gtagaatcct gtaaatcact
    46261 tgcagaatac agcttttcaa aagcttttgc aaatgcttct ttgacttctt ttacaaaaag
    46321 gggttggtca agcagttctt caagtgttgc cattgtagca ggatccactg caggatagcc
    46381 atttgtacca aagtcttctt ttaaagcaga ttgataaaat cgcttcatga cttgcccacg
    46441 tttttcctga ttaccgttga cacttaagta catcataaca gaaatcccat ttttgatccg
    46501 acgctgagcg ataccggcaa attttttgcc atggatgctt aaatcataag ttccaggaca
    46561 ataagaatgg gtgatttcga atgcttgtat cggtgccttt ggaaaagcgt atttagtcaa
    46621 taggagcatt tgctcgtaag cgtcatctat ggttattttt tttgtgtcac tttgggggaa
    46681 aatcaaagaa atattcaaaa ttccttcatc tgatacgatc cctaatccac ctgaatttct
    46741 gatcactgga gcgtaacctg catcttccag tgtttggatc ccgtcactta gaaaaggagt
    46801 tcgaacatcc ttcataccta agatgactgt gcagtcatat tgccagaaat gtaaaaagat
    46861 ttgctggtgt tctttagaat attccgttaa tatatctgtt aatgcaaaag gggaaaagtc
    46921 gtttttttga taaatgtgct gatctagtcg aatcgcaggg ttcattcaac cggctccttt
    46981 gtataaattc atattttcta caatattata ctctaaaatc aaagagttgt tttggaaaaa
    47041 aggttttctg taaaaatgga caagttggtt gacaaaacaa aagaaactag gaacaatgta
    47101 gctatgagaa gtaaaggaga atgaataatg gaattatcac aaggaacacc gatttcaatc
    47161 aaattgcgta caaaagtaaa acagcaaggg gaagtgcaag atttttattt tgatttgaaa
    47221 gggcaaatgg tgacaattgg cgatacgctg tacatacgtt ataaagagat tcaggaggag
    47281 acgggcgatg agattcctgt aacgatcaaa ttagttccag atggtcatgt acagctgatc
    47341 cgtgcaggag aaatgcggat gcgtttgaaa tttggctata aagaacggtt agaaacatcc
    47401 tatcgaacac catacggtat gatccagatt gctactttta cgaaagaact gcatgttagt
    47461 ttgaaagacc gtcccacagc agggaaggtt cgaatcgatt atgatctgtt catgggaccg
    47521 gaaaaaatcg gcgaatatta tttgaccttg gattttacag cgtaaatata aaagaaaagt
    47581 ttgatttttt gtgtctgatt ttgtatgata aggagtagac tgtgaaagga cgtgtcctga
    47641 ttggaaatta gtgtatttga aggtgcaaac aaaagcgaat tatcaatgat cgaagtagca
    47701 cacgcgatct tagaacaacg tggcgatgtc atggattttt ctgatttagc taaccagatc
    47761 caaaactatc ttgggaaatc agacagtgaa attcgtgatt cactcgcaca attttatact
    47821 gacttgaata ttgacggcag ctttatctca ttaggggata atcgttgggg cttgcgttca
    47881 tggtatgcta tcgattcgat cgatgaagaa gtaaaccatg gaatggacga agaggatgaa
    47941 gatactccac gtcgccgtaa acgcaagaaa gtcaacgcct ttatcaatga tgatgaagac
    48001 gcgatcgatt acaatgacga tgacccagaa gatgaagata cagatctgac agaagaagat
    48061 gacgatgatc tgtttgatga cgatgatgat gaagatgaag aaatcgctgc ttataattct
    48121 gacttgcaag aaatcggagc agatgacccg acagatgatg aagaagactt gccaggaaat
    48181 atcgaagaag atttaagtat catagacgaa gacgatgatg atgaagatta tgaagaggaa
    48241 gaattttctg actctgaaaa agaataaaca ttattttctt acgagaggtt gtgacagaga
    48301 cgatcagcct atcagaataa ggaggtgtga cacgattttt gtcacacctt cttttttgta
    48361 tataaaaatc ccaagtaaat acataaaaaa taaaaaaatt gaaactttta gcgcaccttg
    48421 tacgtcatat taagtgaggc agaaaggagt ggagaatgat ttttactgga tataagaaaa
    48481 aacggcagat cgaaaaatac cgtcacctgc tgaatggact catcgaaact gattatcaaa
    48541 aaatgttccg tatcacgatg aattacgtac atgataaaga agaggcctta gatgtgatgc
    48601 aagatagttt tcataaagcc ctgaactatt ttgaaaaaga aaaagagatc gagcatttta
    48661 gtgcatggtt ttatcggata ttgatccgca cggcattaga tagttggcgc agacaaaaaa
    48721 ggacgcctgt ggacttattt gaaattcaag atatccccca acagttacct ttagaaactt
    48781 ccgttgcaga gctccatatg attttagata aaatagacag tccggaaaaa gagattctga
    48841 tcctctactt ttttgaagga tttcgtttga aagaaattgc gactatcttg gatatgaatg
    48901 aaaacacagt taaaacgaaa atgtaccgct cattgaatgc tttacgtcaa atcctagaat
    48961 gaaggagaga agagtgccat gtattcccaa aaagaaaatt taaaaaaact aaagaaaact
    49021 tataaagaac aagcaattcc caaagggctt aaagaggaga tccgccagcg ttttattatc
    49081 gaagagcaat catttataaa agcaaagcgg agaaagagac gtctccaagg gttcggagta
    49141 tctttggcta tcgtaatgat tacaacaagt agccttcttt ttaatagtca agttcgaagc
    49201 tttgcagaag atttacccat cttaggatca gtcattgaat tgatcttagg cgaacgcttt
    49261 actgatcgat ctgaaaaaat cgatattcag gtaccgaaga tcaatacaca aaatgagaaa
    49321 gaaaataaaa caattcatgg attaaatcaa aaatactttc gcgaaggaca agcagatttt
    49381 gaaaaagctg aaaaagaata tggaaatttt gaaacggatc attaccaagt tttgggagat
    49441 tatcagaaaa tcgtagacga taaccggttt ctcgtgattg aaaggcagat cacccaaaca
    49501 gcagcagata gccatgttga aaaaagatac gacacaatcg acaagaaaaa cagtgtccag
    49561 ctctcacttc cacttttatt caaagatgac acctatctat ctgttttgac aaaagaagtc
    49621 aaacggcaga tggccgaaca agtcaaggaa gatccatcta agtactattg gactgagcaa
    49681 gatattcaag aagggacgat agaaaaacca acacttgtta cgcctacacg aagtttttat
    49741 ttgaacaaag agcatcaatt agttttgact ttttctcaat acgaaattgc tcctggttat
    49801 atgggtacac cagaatttgt cattcctaag tccgtgacaa aaacgatttt ggcatcggaa
    49861 gattatttgg atcattagac gagaagtagt tgcgactagc agtttcaatt ttcaagtaag
    49921 tacaagtaaa taataagaag agaaaaagag aaagaagaga tatagtgaaa agaaattatt
    49981 tagttatcag tattattgct ttatccgtgc ttttgttttt cgaaattact tcggtggcct
    50041 ccttgatcac cgaagtaaaa ggacaaacac actcttcgga gcattcgtca caatctttca
    50101 aacaatcaga acaatcaacc attaaaagcc aatcaactga aacagctaac gtcaaagaag
    50161 ccattcaaga atcagcggaa aaggtacaaa gtcagtcatc acaatcaaca caagacagta
    50221 caaaaacgag ccgagatccg gctgtcaaaa gcgtcgcaat cagctttgat gatggaccag
    50281 gagcgaccac aacaccgcag cttctgcgaa tcttaaaaga aaaaaacgtg catgccactt
    50341 ttttcgtctt aggagaaaat acggcgcagc atcctgaaat cgtgaagcag acagctgaag
    50401 cagggcacga gattggcaat cacacatacg atcatcagaa tctggccact ctttctgccc
    50461 agtcgatgac ggaggaagtg acgaaagcag acactgagat caaaaaagtc actggcaaaa
    50521 cccccacttt tgttcgtccg ccctatggtt ctgtcacgaa tgtaggagca acgatcatcc
    50581 agcgaccgat catcgaatgg tctgttgatt cagaagattg gaagacaaga aatcctgatt
    50641 tgattttgca gaagatccaa gcaacagtat acgatggggc aattatttta ttccatgata
    50701 tctatcctga aacgattcgt gccgttcctc aagtgatcga ttatctgaaa gagcaagggt
    50761 atcgcatcac gacagttggg gatttattag ggcatccaac ggctgtcgag aactattatg
    50821 gcagaaacga tcatcggcca gttcagtaag tgagacaagt atctttaata aataacacat
    50881 tttaataagt agttgaacac ataactacct tacttgcaaa ggcttaacaa tatctataca
    50941 ggtacaacaa aaaactgtcc accaattttc tttggcggac agttttcact taaaaataaa
    51001 gaagcaacta ttcgtaaatt atgctaaaat agcagtagaa cgcgtcctga gggaatcgaa
    51061 cccccgtctc aagaaccgga atcttacgtg atatccacta cactaaggac gcaagcactg
    51121 ggataattgt aactaaacag ataaaaaatt acaaggggat tggacaaaaa aatgaaattt
    51181 gagcaaaatt tttcacaaaa acagcagcag acacagaaac tagccatgac gcaacagttg
    51241 caacagtcca tccaagtttt acaatataac agtgaggagc tgttggcatt cgttgagaat
    51301 caagcaatgg aaaatccatt ggtggaagtt gttgagccgg aatggcagcc agattacata
    51361 aaagcttctt catcttctta tgaaggagaa gagacaaatt acctaaatca gattcctgat
    51421 acaaaagggt ctttatttga ttcattgatc gaacaagtcc atttgaatta ccgagatact
    51481 tttttgagga aaatcgttct ttatcttgta gaatacatcg atttgaacgg atttttgacg
    51541 attagtttag aagaagccgg aaaagagatt ggagctacgc ctatccaaat gctcgatgcg
    51601 ttgactctga ttcagcaatt agaaccagct ggagtaggtg cacgtagttt gcaagaatgt
    51661 ctcatgcttc aaacagaacg agatgattat gcgccggaat tagcttatat cgtgttggaa
    51721 gaatgttttg aggaactggt ggagcgaaaa tggaaagaaa tcgcacaacg tttttctgtt
    51781 gatctacatg ctgttcagca gatattcgac tatttacaaa cgttgagtcc aagccctggc
    51841 cggattttcg accgtagcag cgaattgtac attattcctg atgtccgggt attagtggac
    51901 gacgataaaa atgtacaagt tatctcaaac cgaaaaaatc agccaaatat acgctttcaa
    51961 gagagttact tcaaacaaat gtcacaacaa gcagataaag aaactgaaaa ttatttaaaa
    52021 gaacggaaac aggaattcga atggttgaag aaaacgatcc tacaacgtgg ggatacaatt
    52081 ttgcgggttg ctcaagtcat tgtttcccgg caaaaagctt tctttatcga caaggaacgt
    52141 ccaatcaagc ctcttacgtt aaaggaagtt gcaactgaga tagatgtgca tgaatccacg
    52201 gtcagtcgtg cagtcaacgg gaaatatttg gaaacagact tcggtgtatt tgaattaaaa
    52261 aaatttttca ctacacgaat tcctacgaat agtacagaac agacagaaga cctttcagca
    52321 gatacagcga agaaaaagct gcaggaatta gtcgatcaag aagacaaaaa caagccgttg
    52381 tctgaccaga aattggttga attgctgaaa aaagatgaga tagcgatctc aagacgaaca
    52441 gtagcaaaat atcgtgattt attaggaatc ccgagttcat caaaaagaaa acgttatgat
    52501 aactaatcag tcaaaagact agaaaaagta tttcactttt tctagtcttt ttagttgaaa
    52561 aacgagagca aaactgttag acttacagtc gtgaggttga gatttccctt tattgatgtg
    52621 aaattcgaat agttaatttc tttttttatc gtatatgggt cgttttaagt ctacattgga
    52681 cgtaaagtgt ccaactaagg agatcgctat gctggatgaa ataaaaatga ttgaatctgt
    52741 tgcaccagat attatcgaag tgttgcaaga acgctataaa attttacgaa atatctattg
    52801 gatgcagccg attggacgtc gaagtttatc ggaaagtatg gggatcacag aacgagtatt
    52861 gcgaacagaa acagatcttt tgaaaaatct gaatttgatc gacacctcaa aaagcggtat
    52921 gacgctgaca actaagggcg aagaagttta tcaaagcctt gaaaacttca tggatcagct
    52981 tttaggtacg catcaaaccg aacaacagtt ggcagaatat tttggtaacc aacgctgtat
    53041 cgttgtttca ggaaacagcg aagagcagac caaagtggct gatgcattcg gcgaagcgct
    53101 gagcgaagcc ctagaccgac tacttccaga aggcgagaat atcatcgctg tgatgggagg
    53161 aactactatg gctgtggtag ccgaacaatt gtctaatctg gaaaataaga aacggcataa
    53221 cctgtttgtc cctgcaagag gcgggattgg tgaagcgata actgtacagg caaattctgt
    53281 cagtgcaaga atggctgcta aagcaaacgg taaccaccgt gcactttatg ttccggaaca
    53341 attaagtctg gcaacttaca attctttact caatgagcca tctatccaag aagtcttaaa
    53401 cttgattagt gaagcgaatt gtgttattca tagtattggg cgtgcattac acatggcagc
    53461 acgtcgtaag atgactgaaa aagaattagt catgctgaaa caagcaaatg ctgttgcgga
    53521 atcttttggc tactttttca atgaaaaagg tgaagtcgtt tataaggtcc ctagaatcgg
    53581 catagaattg caagatttgg aaaaagtccc gatcatcatg gcaatcgcgg gcggaaaatc
    53641 aaaagctaaa gcaattcgag cttatatgaa aaatgcacca aaacaaacgt ggctcatcac
    53701 tgatgaagct gctgcaaatg agattttaaa aggggtaacc ctttaaaata aatatttttt
    53761 tgattttcat aaggaggaaa tcttaaatga cagttaaagt aggtattaat ggttttggac
    53821 gtattggacg tctagcattc cgtcgtatcc aagatgtaga tggaatcgaa gtagtagcaa
    53881 tcaacgactt gacagatgct aaaatgttgg cacacttgtt aaaatacgac acaactcaag
    53941 gacgtttcaa cggaacagtt gaagttcatg aaggttcatt caacgtaaac ggaaaagaag
    54001 ttaaagtatt agctaaccgc aacccagaag aattaccatg gggcgaacta ggcgtagata
    54061 tcgttctaga atgtactggt ttcttcactt ctaaatcagc agctgaaaaa catttaacag
    54121 ctggtgctaa acgcgttgtt atctctgctc ctggcggaaa cgatgttcca acaatcgttt
    54181 ataacacaaa ccacgaaaca ttaactggta aagaaacagt tatctcaggt gcttcatgta
    54241 ctactaactg tttagctcct atggctaaaa cattgaacga caaatttggt gttgttgaag
    54301 gattaatgac aactatccac gcttacacag gtgaccaaat gactctagac ggacctcatc
    54361 ctaaaggtga cttccgccgt gcacgcgctg ctgctgcaaa catcgttcct aactcaacag
    54421 gtgctgctaa agctatcggt ttagtaattc ctgaattgaa cggtaaatta gacggagctg
    54481 ctcaacgtgt tcctgtacca acaggttcat taacagaatt agtaacagtt cttgaaaaag
    54541 aagtaactgt tgacgaaatc aatgcagcaa tgaaagaagc ttcaaacgaa tcttacggat
    54601 acaacacaga cgaaatcgtt tcttcagata tcgttggtat gacttacggt tcattatttg
    54661 atgctacaca aactaaagta atgacagttg gcgacaaaca attagttaaa actgttgctt
    54721 ggtacgacaa cgaaatgtca tacactgcac aattagtacg tactttagaa tacttcgcta
    54781 acttataaga tagacaaacg tacaagtgta aattatagac atgtacattt aaaagcgggg
    54841 aagcaatcgc gcttcttccg ctttttttga taataagttt attcaggagg aaataaaatg
    54901 gctaagaaaa cagtgaaaga tatcgattta aaagacaaaa aagttcttgt ccgtgttgac
    54961 ttcaacgttc ctttgaaaga cggcgtgatc actgacgaca ctcgtatcaa agcagcattg
    55021 ccaacaatca actacgttct agaacaaggc ggaaaagcaa tccttttctc tcaccttgga
    55081 cgtgtaaaaa cagaagaaga taaagaagga aaatctttag ctccagttgc aaaacgtcta
    55141 ggcgaattat taggtaaaga agtaacattc gtacctgaaa ctcgcggaga acaattagaa
    55201 gaagcaatcc gcaacatgaa tgacggcgac gttgtcgtat tcgaaaatac acgatttgaa
    55261 gatattgacg gtaaaaaaga aagcaaaaat gatacagaac taggtaaata ctgggcttca
    55321 ttaggcgatg tatttgttaa cgatgctttc ggtacagctc accgtgcaca tgcttctaac
    55381 gtaggtatcg cttcaactgg tatcccaaca gttgctggat tcttgatgga aaaagaaatc
    55441 aagttcatcg gtgaagcagt agaagaacca aaacgaccaa tgattgcaat ccttggtggc
    55501 gcaaaagttt ctgataaaat cggcgtaatc gaaaacttga ttcctaaagc agataaaatc
    55561 ttgatcggtg gcggaatgac ttacacattc tacgaagcaa aaggaatcaa gatcggtaac
    55621 tctctagtag aagctgataa agtggaacta gcaaaagaat taatcgaaaa agcaggcgac
    55681 aaacttgtct tgccaatcga caatgtctgt gcaccagaat tttcaaatga tgttgaaaca
    55741 caagtaatcg aaggagacat tcctgatggt ttgatggctt tagacatcgg cccagcatca
    55801 gtaaaattat ttgctgatac attacaaggt gcaaaaacag ttgtttggaa cggacctatg
    55861 ggtgtatttg aaatgagtaa ctttgctaaa ggtacaatcg gtgtttgtga agcaatcgct
    55921 aacttagaag gcgcaacaac aatcatcggt ggcggagact cagctgcagc agcagaacaa
    55981 ttaggatttg ctgacaaatt cacacacatc tcaacaggtg gtggcgcaag cttggaattg
    56041 cttgaaggta aagaattacc aggactagca gcaatcaacg ataaataaac gaccaagttt
    56101 gctcaattct gacgagtaat cgatcaaaag gtgaagggag ttttcatgac tccctagcca
    56161 tttttcaata aattaaggag tgtgttcctc aatgcgtaaa cctattatcg caggtaactg
    56221 gaaaatgaac aaaactgcat cagaagcaaa agaatttgct gaagcagtaa aaaacaaaat
    56281 cccttcaaat gacaaagttg attcagtcat cggatctcct gcattattct tacaagaatt
    56341 aatgacatca gctgaaggta cagaactaaa aatctcagca caaaactgct actgggaaaa
    56401 ctcaggtgcg ttcactggtg aaacttcacc agcagctctt gctgatctag gtgttcatta
    56461 tgtgatcatc ggacattcag aacgtcgtga atatttccat gaaacagacg aagacatcaa
    56521 taaaaaagca aaagctatct ttgcaaatgg tatgacacca atcctatgct gcggcgaatc
    56581 tttagaaacc tatgaagcag gcaaaactgc tgaatggatc gaaggacaaa ttacagctgg
    56641 tttagctgat ctttcagctg aacaagtgtc aaacatggtt attgcttatg aaccaatctg
    56701 ggcaatcggt actggtaaat cagctgacgc acaaatcgct gacgaaattt gtggagttgt
    56761 acgtcaaaca gttgaaaaac tttatggaaa agaagtatca gaagcagttc gtatccaata
    56821 cggcggttct gtaaaaccag aaaatattgc tgaatacatg gcaaaagaaa atgttgacgg
    56881 tgctttagtc ggtggcgcaa gcttgcaagc agattcattt ttgtcattgt tagacgctgt
    56941 aaaataattg ttgaaaattt gcgaaaaatg caagaaatgt ttgcactaat gagcaaaatt
    57001 aactaaaatc gtatatgagg gaaatccctt aggaaaaaac aaactcaaag gagagacaaa
    57061 acatgtcaat tattactgat gtttacgctc gcgagatctt agactcacgt ggtaacccaa
    57121 caatcgaagt agaagtatac acagaaagcg gagcttttgg ccgcggaatg gttccttcag
    57181 gtgcttcaac tggtgaatac gaagcagttg aattacgtga cggagacaaa gctcgttatg
    57241 gcggtaaagg tgtaactaaa gctgtcgaca acgtaaacaa catcattgct gaagcaatca
    57301 ttggctacga tgttcgtgac caaatggcta tcgacaaagc tatgatcgct ttagacggaa
    57361 ctcctaacaa aggtaaatta ggtgcgaacg ctattttagg tgtttctatc gctgttgcac
    57421 gtgcagctgc tgactaccta gaagtacctt tataccacta cctaggcgga ttcaacacaa
    57481 aagtattgcc aacaccaatg atgaacatca tcaacggcgg atcacatgct gataactcaa
    57541 tcgacttcca agaattcatg atcatgcctg ttggcgctcc aacattcaaa gaagctctac
    57601 gtatgggtgc tgaagtattc cacgcattag cttcaatctt gaaagctcgc ggattagcaa
    57661 cttctgttgg tgacgaaggt ggatttgctc caaaccttgg ttcaaacgaa gaaggttttg
    57721 aagtaatcat cgaagcaatc gaaaaagctg gctatgtacc tggtaaagac gttgttcttg
    57781 ctatggatgc tgcttcttca gaattctacg acaaagaaaa aggtgtttac gtactagctg
    57841 actcaggcga aggcgaaaaa acaactgacg aaatgatcaa attctacgaa gaattagttt
    57901 ctaaatatcc aatcatctca atcgaagatg gattagacga aaacgactgg gacggattca
    57961 agaaattaac tgacgtatta ggcgacaaag ttcaattagt tggtgacgac ttgttcgtaa
    58021 caaacactca aaaattatct gaaggtatcg aaaaaggaat tgctaactca atccttatca
    58081 aagtgaacca aatcggtaca ttaacagaaa ctttcgaagc tatcgaaatg gctaaagaag
    58141 ctggctacac agcagttgta tctcaccgtt ctggtgaaac agaagattca acaatctctg
    58201 atatcgctgt tgcaacaaat gctggccaaa tcaaaactgg ttctctatca cgtactgacc
    58261 gtattgctaa atacaaccaa ttactacgta tcgaagacca acttggtgaa gttgcagaat
    58321 acaaaggttt gaaatctttc tacaacttaa aaaacaaata attcttttct aatgaagata
    58381 catgatcgac tattgaaaga tagtcgagta tgatcactta gttaagtaat cgaactctct
    58441 acttgctgaa atatcagcag gtagagagtt tt
//
LOCUS       DASHRQ010000013        52214 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.013, whole genome shotgun sequence.
ACCESSION   DASHRQ010000013 DASHRQ010000000
VERSION     DASHRQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.013"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(28..726)
                     /locus_tag="U2M88_000862"
     CDS             complement(28..726)
                     /locus_tag="U2M88_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEM8373261.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(719..2071)
                     /locus_tag="U2M88_000863"
     CDS             complement(719..2071)
                     /locus_tag="U2M88_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEM8373262.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2294..3694)
                     /locus_tag="U2M88_000864"
     CDS             complement(2294..3694)
                     /locus_tag="U2M88_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEM8373263.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3855..4592
                     /locus_tag="U2M88_000865"
     CDS             3855..4592
                     /locus_tag="U2M88_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEM8373264.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4717..5196
                     /locus_tag="U2M88_000866"
     CDS             4717..5196
                     /locus_tag="U2M88_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEM8373265.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5193..5960
                     /locus_tag="U2M88_000867"
     CDS             5193..5960
                     /locus_tag="U2M88_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373266.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6190..6759
                     /gene="lepB"
                     /locus_tag="U2M88_000868"
     CDS             6190..6759
                     /gene="lepB"
                     /locus_tag="U2M88_000868"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEM8373267.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6863..7465)
                     /locus_tag="U2M88_000869"
     CDS             complement(6863..7465)
                     /locus_tag="U2M88_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEM8373268.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7571..8040
                     /locus_tag="U2M88_000870"
                     /pseudo
     CDS             7571..8040
                     /locus_tag="U2M88_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8146..8730
                     /locus_tag="U2M88_000871"
     CDS             8146..8730
                     /locus_tag="U2M88_000871"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEM8373269.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8733..9806
                     /gene="prfA"
                     /locus_tag="U2M88_000872"
     CDS             8733..9806
                     /gene="prfA"
                     /locus_tag="U2M88_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEM8373270.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9799..10638
                     /gene="prmC"
                     /locus_tag="U2M88_000873"
     CDS             9799..10638
                     /gene="prmC"
                     /locus_tag="U2M88_000873"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEM8373271.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10631..10810
                     /locus_tag="U2M88_000874"
     CDS             10631..10810
                     /locus_tag="U2M88_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373272.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10832..11857
                     /locus_tag="U2M88_000875"
     CDS             10832..11857
                     /locus_tag="U2M88_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEM8373273.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12032..13276
                     /locus_tag="U2M88_000876"
     CDS             12032..13276
                     /locus_tag="U2M88_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEM8373274.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13429..14376
                     /locus_tag="U2M88_000877"
     CDS             13429..14376
                     /locus_tag="U2M88_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM8373275.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14510..15139
                     /gene="upp"
                     /locus_tag="U2M88_000878"
     CDS             14510..15139
                     /gene="upp"
                     /locus_tag="U2M88_000878"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEM8373276.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15278..15520)
                     /locus_tag="U2M88_000879"
     CDS             complement(15278..15520)
                     /locus_tag="U2M88_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373277.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15641..15713
                     /locus_tag="U2M88_000880"
     tRNA            15641..15713
                     /locus_tag="U2M88_000880"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15674..15676,aa:Thr,seq:cgt)
     gene            complement(15832..16470)
                     /locus_tag="U2M88_000881"
     CDS             complement(15832..16470)
                     /locus_tag="U2M88_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEM8373278.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16593..17225)
                     /locus_tag="U2M88_000882"
     CDS             complement(16593..17225)
                     /locus_tag="U2M88_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEM8373279.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17576..17875
                     /locus_tag="U2M88_000883"
     CDS             17576..17875
                     /locus_tag="U2M88_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEM8373280.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            18370..20535
                     /gene="acm"
                     /locus_tag="U2M88_000884"
     CDS             18370..20535
                     /gene="acm"
                     /locus_tag="U2M88_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEM8373281.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(20807..21292)
                     /locus_tag="U2M88_000885"
     CDS             complement(20807..21292)
                     /locus_tag="U2M88_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEM8373282.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    21382..21727
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            21772..22413
                     /locus_tag="U2M88_000886"
     CDS             21772..22413
                     /locus_tag="U2M88_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEM8373283.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            22432..23067
                     /locus_tag="U2M88_000887"
     CDS             22432..23067
                     /locus_tag="U2M88_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEM8373284.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            23085..23900
                     /locus_tag="U2M88_000888"
     CDS             23085..23900
                     /locus_tag="U2M88_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM8373285.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            24219..25655
                     /locus_tag="U2M88_000889"
     CDS             24219..25655
                     /locus_tag="U2M88_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEM8373286.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            25844..28291
                     /locus_tag="U2M88_000890"
     CDS             25844..28291
                     /locus_tag="U2M88_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEM8373287.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            28521..29588
                     /gene="rlmN"
                     /locus_tag="U2M88_000891"
     CDS             28521..29588
                     /gene="rlmN"
                     /locus_tag="U2M88_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEM8373288.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(29781..30884)
                     /locus_tag="U2M88_000892"
     CDS             complement(29781..30884)
                     /locus_tag="U2M88_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEM8373289.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            31437..31985
                     /gene="aac(6')"
                     /locus_tag="U2M88_000893"
     CDS             31437..31985
                     /gene="aac(6')"
                     /locus_tag="U2M88_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEM8373290.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            32531..34192
                     /locus_tag="U2M88_000894"
     CDS             32531..34192
                     /locus_tag="U2M88_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM8373291.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(34262..34609)
                     /locus_tag="U2M88_000895"
     CDS             complement(34262..34609)
                     /locus_tag="U2M88_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEM8373292.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            34693..35634
                     /locus_tag="U2M88_000896"
     CDS             34693..35634
                     /locus_tag="U2M88_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8373293.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            35635..36678
                     /locus_tag="U2M88_000897"
     CDS             35635..36678
                     /locus_tag="U2M88_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8373294.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            36684..37754
                     /locus_tag="U2M88_000898"
     CDS             36684..37754
                     /locus_tag="U2M88_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8373295.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            37744..38697
                     /locus_tag="U2M88_000899"
     CDS             37744..38697
                     /locus_tag="U2M88_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8373296.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            38709..39719
                     /locus_tag="U2M88_000900"
     CDS             38709..39719
                     /locus_tag="U2M88_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM8373297.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(40004..40825)
                     /locus_tag="U2M88_000901"
     CDS             complement(40004..40825)
                     /locus_tag="U2M88_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEM8373298.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(40922..41200)
                     /locus_tag="U2M88_000902"
     CDS             complement(40922..41200)
                     /locus_tag="U2M88_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373299.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            41468..43477
                     /gene="metG"
                     /locus_tag="U2M88_000903"
     CDS             41468..43477
                     /gene="metG"
                     /locus_tag="U2M88_000903"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEM8373300.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(43956..44543)
                     /locus_tag="U2M88_000904"
     CDS             complement(43956..44543)
                     /locus_tag="U2M88_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373301.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            44718..46040
                     /locus_tag="U2M88_000905"
     CDS             44718..46040
                     /locus_tag="U2M88_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8373302.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            46195..46971
                     /locus_tag="U2M88_000906"
     CDS             46195..46971
                     /locus_tag="U2M88_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEM8373303.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            46964..47533
                     /gene="rnmV"
                     /locus_tag="U2M88_000907"
     CDS             46964..47533
                     /gene="rnmV"
                     /locus_tag="U2M88_000907"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEM8373304.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            47526..48410
                     /gene="rsmA"
                     /locus_tag="U2M88_000908"
     CDS             47526..48410
                     /gene="rsmA"
                     /locus_tag="U2M88_000908"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEM8373305.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            48861..49490
                     /locus_tag="U2M88_000909"
     CDS             48861..49490
                     /locus_tag="U2M88_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373306.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(49614..50723)
                     /gene="ugpC"
                     /locus_tag="U2M88_000910"
     CDS             complement(49614..50723)
                     /gene="ugpC"
                     /locus_tag="U2M88_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEM8373307.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            50932..51354
                     /gene="mgsA"
                     /locus_tag="U2M88_000911"
     CDS             50932..51354
                     /gene="mgsA"
                     /locus_tag="U2M88_000911"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEM8373308.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            51677..52213
                     /locus_tag="U2M88_000912"
     CDS             51677..52213
                     /locus_tag="U2M88_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEM8373309.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT        17566 a         9075 c        10626 g        14947 t
ORIGIN      
        1 tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc
       61 caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc
      121 atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc
      181 ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc
      241 accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa
      301 ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta
      361 gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc
      421 tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat
      481 caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc
      541 accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat
      601 ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc
      661 gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt
      721 agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca
      781 atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt
      841 cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt
      901 ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca
      961 tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt
     1021 aattggcatt taataaagcc actaaagaaa atggataagg agctagacca atcatatcga
     1081 ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga
     1141 tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt
     1201 tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat
     1261 tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca
     1321 cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac
     1381 aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt
     1441 gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt
     1501 cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg
     1561 gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca
     1621 ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa
     1681 ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt
     1741 ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa
     1801 ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca
     1861 aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga
     1921 cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac
     1981 ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa
     2041 ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac
     2101 gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc
     2161 ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa
     2221 aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac
     2281 ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc
     2341 gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca
     2401 tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata
     2461 aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt
     2521 ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa
     2581 cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt
     2641 cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc
     2701 cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg
     2761 ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg
     2821 aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct
     2881 tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag
     2941 ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata
     3001 gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca
     3061 tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt
     3121 caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac
     3181 gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta
     3241 attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac
     3301 gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag
     3361 aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata
     3421 tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc
     3481 cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat
     3541 ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat
     3601 aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc
     3661 gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt
     3721 ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat
     3781 acagctttcg attaaaatag aaatggctta taataaggaa aatcctagaa aaaagaagga
     3841 ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa
     3901 taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc
     3961 ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt
     4021 ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt
     4081 atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc
     4141 atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa
     4201 caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc
     4261 gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc
     4321 tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa
     4381 agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct
     4441 ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg
     4501 ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa
     4561 aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa
     4621 aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag
     4681 gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg
     4741 aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat
     4801 agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact
     4861 acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca
     4921 agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc
     4981 tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt
     5041 acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat
     5101 aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc
     5161 caaacgtata accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg
     5221 gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata
     5281 cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa
     5341 accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta
     5401 ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc
     5461 tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata
     5521 atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt
     5581 atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa
     5641 atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact
     5701 atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca
     5761 tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat
     5821 tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc
     5881 tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc
     5941 aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg
     6001 tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga
     6061 gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa
     6121 agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg
     6181 aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct
     6241 attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac
     6301 cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg
     6361 gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc
     6421 ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag
     6481 tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga
     6541 gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt
     6601 gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt
     6661 tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt
     6721 ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg
     6781 tgacatgttt tttgtcacag cttctttttc cgaaaattcc ttcttatttc ttggagttgc
     6841 tcactttttt tgcaatctca ttttaagctt tttggctgat ggataagaaa agcgaagcgt
     6901 ttttgttcat aaatagcttt tccccacgat ggaaggtatg actagtagca gagacaaagc
     6961 ccgttttttc caatcgatta ttcaattctt cttggctaaa gccattatgg actttcggat
     7021 gactaatttg ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca ggagctaaaa
     7081 tttcataaag ttttgtaaga atattctccg tatcaggaat gtgtaaaagt acaagtgata
     7141 gaaggatgag attagcttgg acagcaggaa attcgacaga agcatcagcg tggatcgttc
     7201 ggacattttt cagatctgct gcttggatct tttcttctgc cattttcagc atagtttctg
     7261 aagcatcggc aatgatcagt tctttgaatc tttccgcaag cggtaaacta acaagcccag
     7321 ttccgcctcc gtaatccagc aggacttttt ggtggctgtc gcgcggtaga taagtagtta
     7381 gttcttcgcg aataatcttg gctaatgctt gacggtcagg ggaatcataa tggttagcga
     7441 tggaatcaaa aatatttttt gtcatcaaat agctccttac gtaaaacttt gaaattttaa
     7501 cttattatag cacgtgaaaa gaaagaaagc atgctatact caattcaaaa agggagaatc
     7561 tagaaagaat atgccgatat cgtttgtaaa agatagagag gaaaaaggaa agtgtgtccg
     7621 agaaatatta ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg aaaaatatat
     7681 cgaggaatca agtaaactac ctttatggtg tgaaaaaaga aaagaagaat atttaggctt
     7741 tatcactctt tcacagacaa gtgaagatac tgccgaaatc tattctattg tatgggaata
     7801 aaaaaagact atcatcacca aggattagga acaaaacttt ttaaagaagc agaaggttac
     7861 gcagcaaaac attacaaata tttgcaagtg aagactgtag acgaggggca ctactctata
     7921 tatgatcaaa ctatttgttt ctacgaatca ttaggatttt caaggttgga agtttttcca
     7981 aatttatggg atgaatggaa cccatgttta gttcttgtca aaaaattgga acaaaagtga
     8041 gcaatagatt taatactaat ctgtgcttga tgaaaattct cactgtaaaa aatgctgaaa
     8101 atcaagtata ataccatagg aacaaactgg aaggtgaaaa agagcatggc acaactattt
     8161 tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt cgcacataat
     8221 tatgaagaac aaaataagcc agttgtctta atgacaagcg gtattgatac aagagatggt
     8281 ttaggcgttg tttcaagccg aatcggactg aaacgtaatg cgatgccgat atttgaagaa
     8341 acagacgtat ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt gctgatcgat
     8401 gaagcgcaat tcttaaaaaa gaaacatgtc attgctttta cgagaattgt ggacgaattg
     8461 gatattccgg ttatggcatt tggtttgaaa aatgattttc gaaacgagtt atttgaagga
     8521 tcaaaatatt tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat ctgttggttc
     8581 tgccacaaaa aagcaatcat gaatttacat tatattgatg gaaaccctgt ttatgaaggc
     8641 gatcaagtgc aaattggcgg aaatgaagct tattaccccg tttgtcgaaa acattacttt
     8701 cacccatcaa tgattacaga aggagaataa gcatgtacga tcaattacaa gcaattgaag
     8761 accgttatga agagcttgga gaacttttaa gtgacccaga agtgatttct gatacaaagc
     8821 gttttatgca gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa gtttatcgtc
     8881 gttataaaga agtagtacaa ggaatcaccg atacagaaga actgcttggt gaaaaactgg
     8941 atgatgaaat ggctgaacta gcaaaagaag aattatctga attaaaaaaa gaaaaagaag
     9001 tcttagaaga aaaaatcaag atcttgcttt taccaaaaga tccaaatgac gataaaaata
     9061 tcatcatgga aatccgtgga gcagcaggcg gagacgaagc agcattattc gctggcgatc
     9121 ttttcaatat gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa gtgatggaag
     9181 ctaacgttac cggaatcggt ggatataaag aagtgatcat gatgatcacc ggagacaacg
     9241 tatattcgaa attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt ccttcaactg
     9301 aatcgcaagg gcgtatccat acttccaccg caacagttgt cgtgatgcca gaagctgaag
     9361 aagtagagat cgatattgaa gacaaagata tccgtacaga tatctatcat gcttcaggtg
     9421 ctggcggaca gcacgtcaat aaaacagcat ctgctgtacg tttgactcac ttaccaacag
     9481 ggatcgttgt tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa aaagcaatga
     9541 aaatcttgcg tgctcgtgtg tacgataaga tccagcaaga agcacaaagc gagtatgatg
     9601 ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga acggatccgg acgtataatt
     9661 tcccgcaaaa tcgtgtgact gatcatcgta tcggattaac tatccaaaaa ttagatcaga
     9721 ttttagcagg gaaattagat gagatcgttg atgcattgat catgtatgat caaacatcaa
     9781 aactggaagc tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc acgggcttct
     9841 tcttttttag aggatcatgg gaaagaagca tatagtatcc aatttttatt tttggaacga
     9901 aaacaatgga aaaaacttga ttggctcctt catatgaacg aagagatttc agaagaagaa
     9961 cagcggttga tcgaaaccga tctgcgactt ctgcttgcag atcatccacc tcagtatttg
    10021 ctaggatatg cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac actgatccct
    10081 cgaccagaaa cagaggagtt agtcgaatgg tgtttagacg agacgcctgg tgtaccgtta
    10141 gaagtgatag atattgggac agggactgga gcaattgcta ttagtttgaa agcagcaaga
    10201 aaaaattggc atatatctgc tgtggatctt tcagaagaag cattagaagt agcgaaagaa
    10261 aatgctcaga aagaaggaac gaaaatcagt ttttatcatg gagatacatt aggaccagta
    10321 atggatcagc aatttgatgt gattatttct aatccgccgt acatcagtcg gaatgaatgg
    10381 gaactgatgg atgaaagtgt ccggtcattc gaacctaaaa tggccctttt tgctgagaac
    10441 gatggattag ctatttatga aaaaatagca gttgaagcac caagcgtact aaaatcaaat
    10501 ggaaagattt ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa aatatttcag
    10561 caagcatttc cagataaaaa agtggcgata aaaaaggatc tttttggcaa tgaacgaatg
    10621 atccaagttt atggttgaag ttgtggatga aaaatttttt atttgttttt ttattataaa
    10681 aaaacactta tttgacaagg atgtaaacga ctttttggtt tatcaacaag tttatccact
    10741 attgacaaat agattatcca caaacagtgg aaaacttgag gataaactca gacaaaataa
    10801 gaatgtatga aaaaggggtt gtggataaac tgtggaaacg aaacgatatg atgaaacaga
    10861 attgcaagag gcagccgaag cactgaaaga aggagaactt gttgcttttc ctaccgaaac
    10921 agtatacgga ttgggcgcca acgctctttt accaaacgct gtcaagaaag tgttttctgt
    10981 aaaagggaga ccgcaagata atccattaat cgtccatgtc gcctcttttg aacaagtaaa
    11041 agagtatgtg gacaactttc atccggcagc agagaagatt gtgaagaact tctggcctgg
    11101 accattaact ttgatattta agatcaaaaa agacactttg ccctcagttg tgacgggagg
    11161 attatccaca gctgcatttc gtataccgga taacaaaaaa acactagaag taatcgaact
    11221 ttccggtgtt ccgcttgtcg gtccaagtgc aaatacgtcg ggaaaaccaa gtccaacgac
    11281 tgcagatcat gtatatcatg atctacaagg aaaaattacc gggattattg atgatggagc
    11341 tacgcgaatt ggtgtagaat ccactgttct tgatttaagt gatccaacag ccatgcctat
    11401 gattttacga ccaggtgcag taacgaaaga acaaatcgaa gcagtcatcg aatctcctgt
    11461 tgctattgat cagcatcttg taaaagaaaa tgaaacacca aaagcccctg ggatgaaata
    11521 caaacactac tcacccgata cacgtgttct gatggtcaga gatggcgatt ggtcaactgc
    11581 agttcaatgg gcaaaaaata agaagatccg tgcaggtgtc atagcaagcc ccgaaattgc
    11641 ggatcaagtg cggacagata ctgcagctgt ttatatgtat aacgacaact cagtagaagc
    11701 ggcggctaaa ggattgtttg caggtctaag aggattagat gaaccaacac ttggattaga
    11761 tttgattttt gtacaagtgt atccagaaac tggattgggg aatgcttaca tgaatcgctt
    11821 gaaaaaagca gcaggacaaa attattttga aaaatgatca attggttatt gtcgcattct
    11881 tttagtatga tacaatgcta tcaagtaaat cttctatcag tgaacgaaaa actaagcgta
    11941 actaatcgta atagaagaga tacgaatatt tggcaatttg tttttgatgg gaaaaataaa
    12001 attgtaggaa aataaaaagg agctgttgtt tgtggtagat tacaaaacgt ttgaccctga
    12061 tctttgggct gcgatcgcca aagaagaaga aagacaagaa cataatttgg agctgattgc
    12121 ctctgaaaac tttgtctccg aagcagtgat ggctgcacaa ggaagtatct tgacaaataa
    12181 atacgcagaa ggttatccag ggcatcgcta ttatggtggg tgtgaatttg tcgatatcgt
    12241 tgaaaattta gcgattgacc gtgcaaaaga attattcgga gcgaaatttg cgaacgtcca
    12301 gcctcactcc ggctctcaag ccaacacggc tgcttatctc gctttggtag aaccagggga
    12361 cacgatctta ggaatggatc tttccgcagg cggacatcta acacatggat caccagtcaa
    12421 cttcagtggc aaaacctatc actttgttgc ttacggtgtt gatccgacaa cagaagttat
    12481 tgattataat gtggtacgta ttttagctag aaaacatcaa ccaaaattga tcgtagcagg
    12541 tgccagtgct tacggacgaa cgatcgactt tgcgaaattc agagaaatcg cagatgaagt
    12601 aggcgcaaaa ctgatggtag atatggcaca tatcgcagga ttagtagcag caggacttca
    12661 tcctaatcca gttccttatg cagatatcac aacgactacc actcataaaa cattgcgtgg
    12721 ccctcgaggc ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa taaatagtgc
    12781 ggtatttcct ggtattcaag gcggccctct agagcatgtg attgcgggta aagcagtagc
    12841 attcaaagaa gcattagatc ctgcatttaa agaatatagt gaacaaatca tcgccaatgc
    12901 aaaagcaatg gtcaaagttt tcaatcaagc aattggaacc agagtcattt caggggcgac
    12961 agataatcat ctgatgttga tcgatgttcg cgagttaggt ataaatggaa aagaagcaga
    13021 gagcattttg gacagtgtga atatcactgt aaataaaaac tcgattcctt ttgaaacact
    13081 aagtccattc aaaacaagcg gtattcgaat cggcacgcca gcaatcacta ctcgcgggtt
    13141 caaagaagaa gatgctgtaa aagtggctga actagttgtc aaagcattac aagcaaaaga
    13201 tgataacgct caactagacg aagtcaaaac aggtgtacgc gaattaactg aaaaatttcc
    13261 gttgcataaa aaataaaaat ttaaattcca ttagtaaaca gaaaagttca atgttgggct
    13321 tttctgttta ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat
    13381 atgaggtgta attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt
    13441 atctcgttag aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc
    13501 tatgaactag caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg
    13561 aacaaatatt ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc
    13621 aatattcgtc tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt
    13681 catggagcag gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt
    13741 gcggttcgta cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa
    13801 tatccaacag caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag
    13861 tcaaaaaatc tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg
    13921 gctacagtca tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg
    13981 ctgtattatc cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa
    14041 aactattttt tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa
    14101 gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat
    14161 cttccttcag caatgatttt aacgggagaa gcggatgtat taagagatga aggtgaggct
    14221 tatgcgcgca aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc
    14281 atccatgatt ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg
    14341 gcattatcta cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact
    14401 tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat
    14461 tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt
    14521 tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg
    14581 cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt
    14641 atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca
    14701 aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat
    14761 ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg
    14821 agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc
    14881 acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat
    14941 cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc
    15001 tgagggagta aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct
    15061 agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg
    15121 tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc
    15181 ttttttggtc atttttaacg tttgatgcag aacagctttt tcacagcttt cgtttgcata
    15241 taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca aatctttttt agttctttgt
    15301 agttaacgat tactacaccc ttactttgcg tcatcaagtt cgcattttct gcagtttcat
    15361 caatctcaac aacagcggaa tttttcaatt gctttgaaat cgttccagtt tgcgacttgc
    15421 cgtgaattgt gaacattacg tgagtattga tcttgaattt ttcaccaatt tccgatggta
    15481 cgatagttgt atcaaatttt ccaaaagttg ccattttcat aaaacctgcc tttcacatca
    15541 attataccac gttttatgaa aaatatcatg aaattaaact aattttccat cggactcttt
    15601 acaatcagcc aaatagagta tataatttct tttggataag gccagcttag ctcagttggt
    15661 agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc cctatagctg gcattaaaag
    15721 agaagacaga tatatcaagg atttttaatc cattgataca tctgtctttt tgtttaaagg
    15781 aaataaagta tgctttctta gcaatagagc atgtattatt tctataaaaa tttaggagaa
    15841 taaaacgact agcaatatag ctgtgaaatc aaatatcaca tgaactgccc aacttgtaat
    15901 gatcgagttg cttttaatgg cagcttggtt gaataacaat cttgccaccc cttgtatcaa
    15961 aaggatgtgt gctacagtgt gtaaaatatt tccattatca taagtactgt aatggataaa
    16021 tccaaagatg attgctgaca agaaactagc gacaggaatg ctgaacttac tagaaaaaat
    16081 agcgagaaaa tacactgaga acaattcttc tccaagtatc atgataggca aaataataaa
    16141 taataacggg ctgaaagcct catttattgg attgcctgtt aaaccccatt tcaaaatttg
    16201 ttgcaaaaat accgagactg cggcactgat tactacattg ataaaaaaat atttggctat
    16261 atttttgaca ggagcgacag gtttcgagaa catttttttc gtatcttttt taccaaaaac
    16321 aagtgtaaat gctataaatc cagtgatagt taaagaaaaa atgctaataa tggatggcgc
    16381 aaatagcaac cctaacaaac cgagtacaac gacaatagtg cccaatatcc cttttttaag
    16441 ttctattttt tttcctaaaa tgttattcat gataataact cctactattc agtttttgaa
    16501 gatagagtca gtatacatga agagttaaaa taggcaatgt ttttttatgg atatttgtat
    16561 tgctaaaaat ttatcttata tattcatttt gattatacta cttttatttg taaaaatttt
    16621 ctagcaaaac caagtgcttg caactgatta agcgtacaat tttttagatg ttccgagcgt
    16681 acttcaatca tttcaaagga gttgctactt aaatcgatat tttttaaatc agtatcaaaa
    16741 aagctagcat tttctaaatc attagtagaa aacattattt tagtgaggga acagtctaca
    16801 aaatctgcgc ttgataagac agagtgctca aaggtacttt cttttatgtt tgaaccagat
    16861 aaatttacat agtccatttt acaattctcg aataacgtgt tcccaatata actttccatc
    16921 aatttagtac ctgttaattt acaattgaca aatttcacgc gatgcaacat gctttttgtc
    16981 aatatacagc ctgaaaaatc acagttttcg aatatacagt cggtaatctc cattctttga
    17041 aaatcattgg aagcaaaaaa tgcatacttg aaaatagatt tgtaaataat tggatggcag
    17101 attatttggt tttcgatgac ctctttttgt gaaaggatat tatacagttc aaaatcttcg
    17161 ttttcataga tatgaaaaaa ggatccttta ttcaagcttg cttcattaac tttaggaaaa
    17221 atcatattta attacctcca ttttcataaa gtcatattaa caaaagctaa accaacttac
    17281 attagcagaa aatgagatag gttttcgcct ttttcgataa cgatagtgtc gctatctctt
    17341 gaaacgttct tagaattgcc ctgagagaca caaattaggc gtaattctaa taaaaatctt
    17401 agaataaaaa tttttactga tttaatttag aatgttttta aaataaaaat cgttgccata
    17461 acagtgtatt gaaaggtttt aacaatccag taatgattta aaaaaatcta gatgtagcgt
    17521 atactttgtt tatagaatgt agtgggaaga aatatatttt tgggaggaaa tcgtgatgtc
    17581 taaaagtgaa gaaatagaaa acatggtact aaaattatat gatctcgttt taaatccaga
    17641 gatcaaagaa aaggaacgta tacttttgat agacgcaaag acaggcttag aaaaaggtca
    17701 gtattatcct aaagtcataa ataatttgga acgtagttta cgtccgttag caatcagggg
    17761 agaattgtct aaaccagtat caccgtttta tatggagata tcaacaatag gaaaatttga
    17821 aaaagaatta ggtcgaggaa tggcttcggc accgattact tttgggcatt tataaaaacg
    17881 agttattcat ctcaatgaat taaaaagtat agtcaatctc ataatgcagg ctaggttcat
    17941 ttatttgaac agagtctgta tttttttatt ctaaataaag aaacagatgt ttctttatat
    18001 tgtattttat cctttaatta acacaaaaac agctagtata aactaataat gttaattaaa
    18061 agtgtatttt atgaaactat cgttatttat taatgttgca tttttgtaag ttaaaaattg
    18121 gaaatataga ttgaaaaaat gaaagcaata agtaaaataa aatgaatata tgcgaaaaaa
    18181 taatttacat taggaaaaac cttgtaaaaa cagttttttg gcatatttat tttttataaa
    18241 tatttgtaaa ataactatca attttttagt taaaagattg gaaatgagtg ggggaggaat
    18301 tgaagaaagg atttttgaga gatgatatag taggttttat ttttagttaa aataggggag
    18361 atagaaaaaa tgaaaaagtg tagaaatatt attttatcaa tgttatttat tattactaat
    18421 ataacatcac tgattcctgt tcatgtctat gcggatgcag gcagagatat cagcagtaat
    18481 gtcacttcgt tgaccgtgga cccaacgaat attacagatg gaggaaacat aaaagtaaaa
    18541 ttctcgtttg acgaaaaaaa acaaaatatc caaccaggtg attatctttg gatcaattgg
    18601 ccaagtgaag gaaatattcg cggtgaagga ttccaaaaag aaatcccatt aatgattgaa
    18661 aataaaaatg ttggaacttt aacggtcaga aaagattctg cgcaggttgt gtttaatgaa
    18721 aatattaaaa acctagactc agtagaaggt tgggggcaat ttgagataca ggccagaaac
    18781 gtaaccgata ccggtgaaga aaatacggga ccattcaccg tgacgagcgg tgataaaaca
    18841 gctactgtaa atgtgacaaa gccagcttct ggttcttctt ctagcgtttt ttattataaa
    18901 acaggagaca tgctacctga agatactaag cacattcgat ggtttttgaa tattaacaat
    18961 aacggcactt atgttgaaca accagttaaa atcagtgatg aaatccaaag tggacaaaga
    19021 ttagatccaa gtacgtttga aattaatcaa atacacttag gtgaacagaa ggtttataga
    19081 ggagaagagg gaatccaaca atttttacaa gatttcccca gcgctacatt taatttttca
    19141 gtaactgata attacattga aataactata ccaaaaaact ttgtcaatct taggaaaata
    19201 atggtgagtt ataagacgat catagaaaac ccagaacaaa taaattttga gaatcattcg
    19261 gaagcttggt ttaaagaatt taataaacct gctgttgatg gagaaagttt caatcataca
    19321 gttaaaaata tttcagcttc tggtggagtc aacggtacag ttagaggcga attgaagatt
    19381 ttcaaatata tcaatgacac agagattggt atcccaaatg tgactttcga attaagacga
    19441 gcagatgaac agcctattca aggacagtct agtatcctgt tgacttcgaa tgaacaggga
    19501 gaagctagta tcaaaggcct gcaagtagga gactatgttg taaaagaaaa agaagcaccc
    19561 aattggattg attttgatcc actaagttca aatgaactca aattctccat aaacgaaaat
    19621 gatacagagg gagtcagttt acctatttat aacaagaaaa aggttacgaa catcacagca
    19681 actaaaattt ggaacggtgg aaccactcca agaccgagta tttattttaa attattcaga
    19741 gcaagtacaa acaattggga accagttcct gatgcagaaa caaaacgatt agacaatgga
    19801 ataacttcag tgacttggga agatatccaa cagtatgatg atagtggaaa cgaatatacg
    19861 tttaaagtgc aagaagtaga tcaaaacgga aatgattatg tcccttcagg ttatcaaaaa
    19921 attgaaaacg ggctagtcgt tacaaatgag aataaagaag tcatttcagt ctcaggacaa
    19981 aaaacatggg aagacaatga gaaccaagac ggcaaacgac cgacgacgat cacagtaaat
    20041 ctattagccg acggtcaacc gatccaacac aaagaagtaa gtgaaaaaga tgattggcgc
    20101 tatcgtttca caaatctgcc aaaatacaga gacgggcaag aaatcatcta tactgtaacg
    20161 gaagacaacg tgcccgaata cagtacgacg attgacgggt ataacatcaa aaacagttat
    20221 acaccggaaa aaacaaatat atctatacca ggtagccata taacaccgtg gaaaaatttt
    20281 ccagagaaga cagagagaga gaaaattaga aatctattct ctcaaaaatt gcaaatattt
    20341 gctcatacag gagaatccag cgaacaaaga agcaattata ttagtaagag agctgtacct
    20401 aaacagataa caaataaatc aaaaagcatt cttcctaaaa caagtagtaa aaaatctagt
    20461 tttgctgtaa tcattggttt attgatagta actatctgtg ctgggatatt cttacaaaaa
    20521 tataaaaaag tttagttaga atataatatt ttttgttaaa tatgactgaa atcaaaagaa
    20581 taaaattccg atttataatc gaaaaaagaa gcatttttat atatgcttct tttttcgtag
    20641 aatcaataaa tatggatttg gctaactatg gaagaatact cgtattagtt ttcagcgttc
    20701 atactagttg ttacattgtt tgattggcca aaagaatttc tgatatattt tgtgctaaat
    20761 aaagaaaaag acgcttgaat tgggaaataa gaaaactact ctatcattac aaattttcat
    20821 aaactggcag atgttctaag taaacaagta tcttttcaaa gtcggaaaaa tccataatca
    20881 acgtagtggt attgatattt ggatgaaagc ctacggtatc ttgacgatca atagcagcat
    20941 cgatcacgac ttggattttt ttctctttgt cgtgcatcaa tgctaatgga gtcactgtac
    21001 ctgctggaac gcctaggagg tcaggaagct gttcttcaga aacaaaagat aagcgctttt
    21061 cttttaattg ttcagctagt tgttttaaat cggcgtgctt ttcatcaggc aagatgacaa
    21121 gatagatttg ttttcctttt cttgatttca ataatagatt ttttacttgc ggaccaggta
    21181 gggaaaaagg gacatttttg actgacgtaa tcgcaggatg atcgactttt tgaaaagaga
    21241 tacccagttt ttttagtagt tcataagctt cttgttcggt tgcacgactc atcgcgcatc
    21301 accctccttt ttttattagt gtatcataaa aaagagttga caaatgaaaa ataatcatgt
    21361 aaattacacg taaagttaaa acaaatgaca tcgaaaagaa gagtagccgt taggtgtttt
    21421 tagagagtct ctggttggtg gaaagaggca gcagttaacc gtgaaacaca tcttggagtc
    21481 gattttctga agcgtatagt aggaaaatcc ggggagcccg ttacagctgc ggtcgttgct
    21541 agatgaccag ataaggctgg cttttccgtg aggagtcggt aaaaaaaggt ggaaccacgt
    21601 gattaacgtc ctttgatctt cggatcaaag gactttttgt tttcatctga acggcaacga
    21661 ctgtgtgagg ttttttctgc aaggaaaaaa taaacagagg tggaaccacg tataatacgt
    21721 cctcttggca tttgccaaga ggattttttt attttaaagg aggaaaagaa gatggagtat
    21781 ttgattgaag ttatgccgca attattagta ggagcactca caacattgaa attattttta
    21841 tttaccttga ttggctcatt accattaggg attgttttag cattaggatt gtctttgcat
    21901 tttaagccgc tacgctactt gcttcaaaca tatgtatggg ttatgcgggg aacaccgctg
    21961 ttgttgcaac tgatttttat tttttatggg ttgccgacga ttgggatcgt ttttgatcgt
    22021 ttcgaagcag cactcattgc ctttatctta aactatgcag cttattttgc agagatcttt
    22081 cgtggaggga tacaatcgat tcctcaaggt caatatgaag cagcgcaagt tttgcaatta
    22141 agtccctttc aaacggtgac gaaaatcgtc atcccacaag tgacaaagat tgttttacct
    22201 tctgtaggga atgaagttat caacttggtc aaagattctt ccttgatcta tatattaggt
    22261 ttaggtgacg taatgcgtgc tgggaaaatc gcgatggaac gcgacgcgac tttactgcct
    22321 ttgctaggcg tggcaatcat ctatcttaca ttgactggta ttctaacaat cataatgaaa
    22381 caagttgaag gaaaattaaa ttattaccgc taactgatag gaggaagaaa tatgattgaa
    22441 ttgaagaaag tgaacaaagc atttggtgaa aaaaaaataa tcaaagattt gaatctaatc
    22501 attccagatg ggaaaacatt agctatcgta ggtccttccg gtggcggtaa aaccacgctt
    22561 ttacgtatct tggctggctt agaaagttct gacagcggag aattttattt agacggtgta
    22621 ccgttcaacc cattcgagct taaaatgaaa gaacaagttg taggagtcgt attccaagac
    22681 ttccagctat ttccacatct gaatattttt gaaaacatta ccttagcacc aaaaatggta
    22741 ttgaaacaga caaaagaaga atacaccaaa aaagcagaag aattagcggc ttcattaggg
    22801 attagtgatt tattgaatat ctatccttat cagctttctg gcggacaaaa acagcgggta
    22861 gcgattgcca gagcattggc tatgaatcca cgggtattgg cttatgatga accaacaagt
    22921 gctttagatc ctgaattacg ccagcaagtc gagcgtttga tcctatcatt gaaagaagat
    22981 ggggtaaccc aaattattgt cacccatgat ttagtatttg cagaaaatgt cggtgatcaa
    23041 ttactaaaag tttccccaat tcattaagaa aggagaagat agagatgaaa aaaaccagtt
    23101 ttctattcgc tttgatcgca ggtcttcttc tttttgcagg ctgttcgaac aaaaaaactt
    23161 cagcagataa ttgggcgact atcaacgaag agaaaaaaat cgttataggt ttagacgata
    23221 catttgttcc tatgggattt agagataaag acgggaagct cacaggattt gatattgatt
    23281 tagccaaagc agtctttgaa caatatggga ttacggtaga ctttcagccc attgactggt
    23341 caatgaagga atttgaactg aacaatggaa cgatcgattt gatatggaac ggctattcca
    23401 aaacgccggc aagagaaaag aaagtgcaat ttacaaaacc ttatatggaa aacgatcaag
    23461 tgctgatcac acctaaagaa tcaggaatca caaactttgg acaaatgaat aataagagat
    23521 taggtgcgca aaatgggtca tctggttatg atgtatttac gaaacaacca gaagtattaa
    23581 aagagatagt caaaaaccaa gatgctgttt tgtatgatag ttttaatgaa gcattgatcg
    23641 atctgaaaag cagacgggtc gatggtctgc tgatggataa agtatacgca gactattatt
    23701 tgaaacaaag aaacgaacta gaagacttca acataacaaa aggttcttat gagagtgagg
    23761 attttgcagt tggtgcacgc aagtcagata gtacattagt gaaaaaaatc aatgaagccc
    23821 tagatcaact agagaaaaca ggtgaattcc aaaaaatatc cgataaatgg tttggcgaag
    23881 atgttacgcc gagaaattaa caaatagggg actgtgacaa aagacgtgtc acagtccctt
    23941 tttccgaata aacagtgttc tcctgccaac tccttgtgac tcggagctga atggcaggct
    24001 cacaacctct tggtaaacgg tgttcaaaag ccagtttttc ggtattaagc caaatttatg
    24061 caaaaatgta gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcgacttt
    24121 ttcacaacct cctttacata attttatttt gttgtggaag gggtttcgtg ttattctaaa
    24181 agaggaacga ttgaaggtta taggaaagga agagatgaat ggaacacaaa gaacttacct
    24241 catttccaaa agattttctg tggggctctg cttctgcagc gtatcaagta gaaggagctt
    24301 ggcaagaaga tgggaaagga gaatcagtct gggatcgatt tgtacggata cctggaaaga
    24361 catttaaagg aacgaacggt gatttggcag tagaccacta tcatcgttat aaagaagata
    24421 ttgctttaat gaaagaacaa ggtctgaaag cttaccgctt ctctgtagct tggacacgga
    24481 tttttcctaa tggacgagga gaagtcaatc aagctgggct cgctttttat gagagattga
    24541 tcgacgaatt gattgaaaac gatattgaac ctatgttgac tttatatcat tgggatcttc
    24601 cacaagcatt gcaagatgaa tataatggtt gggaatcacg tcagatcatt gaagatttca
    24661 ctcattatgc agaaacatta tttgaggcat tccgcgggaa agtccattac tggattagtt
    24721 tgaatgaaca aaatatcttc acttcattag gttatttgtt agcagcacat ccgccaggtg
    24781 tgacagaccc gaaacgaatg tatgaagtta accatattgc taatttagca aatgcatctg
    24841 tcatcaataa atttcacgag ttagaaatgc ctggaaaaat cggaccaagc tttgcgtata
    24901 cgccaaatta tccaattgac agtaatcctg aaaatgttct ggctgctgaa aatgcagaag
    24961 acctgatggc caattattgg atggatgtct atatgtgggg aaaatatccg attgctgcca
    25021 tgaggttctt agaagaaaaa ggatgggcac caacgattga agctggcgat gcagaattgc
    25081 tagaatctgc taaaccagat tttcttggga tcaattacta tcaaacggcg acgaatgcat
    25141 tcaatccatt aaacggagtt ggtgctggaa aaatgaatac gacaggaaaa aaaggttcat
    25201 cagaagaaac tggagttcct ggaatgtata aaaaagttga gaatccattt gtcgaacgaa
    25261 caaattggga ctgggagatt gacccagaag gattaaggat cggactgcgt cgtatcacaa
    25321 gccgttaccg catgccggta ctgattacgg aaaatggctt aggagagtat gacaagttga
    25381 cagaagataa acaaatccat gacgactatc gaatcaatta cttacaaagc cacgtcaaag
    25441 ccatcaaaga agcaatttcg gatggagcag aagtcctagg ctattgcacc tggtcttata
    25501 cagatttgct tagctggctg aacggttatc aaaagcgata tggatttgta tatgttgatc
    25561 aagacgaaac acaagaagga tctttggaac gatataagaa agacagttat tactggtacc
    25621 aaaaactgat tgaagaaaat ggagagaacc tttagttcac atacttaaaa caagaaggta
    25681 cggctggata tggccgtgcc ttttcttgat aaaatcattt agaaattaag aaaaactgta
    25741 aaataagctg aaaaatgaaa gaaagcgtca gttctttcaa acaaaacttt agttccctta
    25801 cttttttttg tataatgaaa cttgatgaaa tgagggataa taaatggcac aaaagaaacg
    25861 gcctactaaa aaaaggaaaa agacgaaaaa aacgaaaaag caaaaacagc agcaggaaca
    25921 aatgctgacg attggtgttg ggtttgcctt gattttattt gctgtcttcg gctttttaaa
    25981 attaggtttt ttaggaatat tgattgcaaa cggctttcgg atcattgcag gaaatactta
    26041 ccaaatcctc tgtcttttat tagccgtatt aggattttgg attgtcatta agaatacaga
    26101 gttttcaatt ggtaaaaacc gacgctggtt tggcggcata ctcttttatt tcggtatctt
    26161 attattgtta catgcccatt tatttggcaa actccatact ggggaaccaa atatcatggg
    26221 gacgacatgg gatttgttag ccagtgatat aaaacaaagt caagtagaca ataacgttgg
    26281 tggtgggatg attggagcaa tcctttatca ttttacttac ttcttgattg cacagcctgg
    26341 aagttattta gcagcagtgc tattactggc aggaggagca ttcctgttca gtaatctgga
    26401 aggctatcag ctgttaaatg gtatccaatc aattggagaa cgagtacagg aattattgga
    26461 aggtgatcct gccaagcaag ctcgaaagca agcggcaaag gaagagagaa tgaaacagcg
    26521 cgcagaagcg aaagaagcaa ggcgcttagc tgcaaaagaa gctgcagaaa aagaagctgc
    26581 agaatatgag aagaaaaaag cggtgagcca gcaaagggaa aatactgctg atgaatggca
    26641 ggaaccagaa aatacagagc cggagcagct aagttttgtt ccaatcgaca gttttcaaga
    26701 aaatatccat cctgcgaatc tagagaaacc agtaccagat acaccaaaac agaccaatac
    26761 agcagaaggt ttttcagatg agttgccaga ggatgacggt acctcattag aatttgagat
    26821 tgaagcagaa caggaaaatc aagactatga attaccgaca gttgatttgc ttgattctat
    26881 accaactgtg gatcaaagtg atgagtataa aaaaattgaa aaaaatatcg gcgtattaga
    26941 acaaactttc caaagtttcg gtgtcgatgc caaggtcgtc aaagccagct taggaccatc
    27001 cgtcacaaaa ttcgaagtcc agccggctgt aggggtaaaa gtaagtaaaa ttgtgaactt
    27061 aacagatgat atcgcgcttg ctttagcggc gaaagatgtc cggatggaag caccgattcc
    27121 aggtaaatca ctgattggta ttgaagtacc aaatggaaag atcagtatgg tttcctttag
    27181 agaaatcatc gaagcacaac caaaccaccc agataaatta ctagaagtac ctttaggacg
    27241 tgatgtttct ggacgggtac aaacggctga tctttcgaag atgcctcact tactcgtggc
    27301 tggttcgact ggtagtggga agtcagtagc aattaatggg attatcacaa gtatattaat
    27361 gcgtgctaag ccccatgaag taaaactgat gatgatcgat ccgaaaatgg ttgaattgaa
    27421 tatgtacaac ggcatacctc atctgttgac gccggtcgtg acgaacccta gaaaagcagc
    27481 gcaagcctta caaaaagtag tacaagaaat ggaagaacga tatgaaaaat tcgcggcaac
    27541 cggagtaaga aatatttctg gttacaatga atttgttcaa cagaaaaact tggaaaacgg
    27601 aacgaaacat ccaaccttac cattcatcgt agtgatcgta gatgaattag ctgatttgat
    27661 gatggtagcc agcaatgaag ttgaagatgc gattattcgt ttagcgcaaa tggctcgggc
    27721 agcggggatt catatgatcc tagctactca acgtccaagt gtggatgtta tcacaggaat
    27781 catcaaagca aatgtacctt ccagaatggc atttgcggta tcaagcggta cagattctcg
    27841 gacgatcatt gattctaatg gggcagaaaa gctcttaggg cgaggagata tgctattctt
    27901 gccaatgggt gagaataaac cgatccgtgt gcaaggagca ttcatttctg accacgaagt
    27961 agaacgggtc gttcaatttg ttacagatca gcaagaagct cattatgaag agaaaatgat
    28021 gccgacagat gaagttgaaa cgacaggtgc tccagaacag ccacaagatg agctgtttga
    28081 agaagcaaaa gcacttgtcg ttgaaatgca aacggcaagt atttcccttc tccaaagaag
    28141 atttcggatc ggttacaacc gagcagcacg tttagtagat gaactggaag ctcatggagt
    28201 tgttggcccg tcagaaggaa gcaagcctcg gaaagtattc attgagcagg aagaaacatc
    28261 agaaacgttg ccggacgata atgacatgta aaacttgtgt gatgaagggt tgaaaaacga
    28321 ataaccaata aaaagacaag gcatgatgat accaaaagaa aaaatggttt ttttccatct
    28381 tttctaatac ggtatgatgt cttgtctttt tagtttttgt gagattaaag ttctattcga
    28441 tttatttgtg aagtattgca ttaaggaaat gaatcatgta tagtatgtag cgaaactttg
    28501 agaaaaagag ggaaaatact ttggaaaaac agtccattta tggcttgacg aatgaagaat
    28561 taatcaattg gtttatagaa aacggcgaga aaaaattccg agctgcccaa gtttgggaat
    28621 ggttgtatca aaaacgtgta agtaatttca cagaaatgac gaatctatcg aaacaattga
    28681 tcgaaaaatt atcggagcat tttatcatca atcctctaaa acagatggtc gtccaagaag
    28741 cttcggatgg tacagtaaaa tatttgtttg aactccctga taaaaacatg attgagactg
    28801 tactgatgag acaagaatat ggcttgtcag tttgtgtgac gacacaagta ggatgtaata
    28861 tcggctgtac gttttgtgca agcgggctgc tcaaaaagaa ccgtgatctg actgctggag
    28921 aaattgttgc acagatcatg atggtccaac attattttga tgagcgcaat cttggcgaac
    28981 gcgtttcaca tgtcgttgta atggggatcg gtgagccatt tgataattac gacaatgtaa
    29041 tggacttttt gcatatcatc aatgatgcca aaggattggc aattggtgct cggcacatta
    29101 cagtttccac tagtggatta gcacacaaaa tcaaggaatt cgctaataac ggcttgcaag
    29161 tcaatctggc tatttccttg cacgcgccga ataatgaggt gcgaacatcg atcatgcgga
    29221 tcaaccgaag tttcccaatt gagaaattga tggaagctgt ggatgagtat ttagagaaaa
    29281 caaaccgtcg aatcacgttt gaatatatca tgttgaatca agtaaatgat agaccagagc
    29341 atgcgcaaca attagcagat ctattaaaag ataagaaaaa attagcatat gtcaacttga
    29401 ttccatataa tccagtcagt gaacatgatc aatattctcg cagtccaaaa gctgatgtat
    29461 tgagatttta tgatgtattg aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg
    29521 gtacagatat tgatgcagct tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg
    29581 ccaaataatc gtatcctact cgttttttca attaccataa aaggagcagt tttcttaatt
    29641 aaggaactgt tcaacttaaa taagagaaaa gattctatag cgaaagctat aaagcttttt
    29701 ttgacaagaa aaaccctcat caagcaaaga aatgtcctca atttgatgac agcctgcttg
    29761 atgggggttt tagcaattat ttaactgatt ttcttatatc cttgctggct ttcttgtgta
    29821 gcatgataag tttctacgtc aagaatggcc tgtttaaatt tttctcgggt gatttcataa
    29881 ggcacatgtt taatatcatt tgttgccatg gctttatcta agattgcttc taaatcggtc
    29941 gtgacattga gattcatgtc ggttaacgat actggaagtt ggttcttttt cataaacaca
    30001 tagattttat caaacaaatc gaactgctga tccatcatca ataaggtcag tacaccataa
    30061 ctgacagatg ctccgtgaag gtatccctcc attttttcta gaacagtcgt accgtaatag
    30121 aatgagtgtg ccatagcaga attatagtta ttgttaacta aaacagaaac caatccggta
    30181 gtaacaatga tatccgaaac gacttgttca attgcatatg agggacgatc gtcggcacta
    30241 tctttcaatg cttgcgtacc ataagtcaag agacgatcaa tgcacatttt tccgatttgg
    30301 attcctaatc tatccgtgta gcctagatca tcgccacgag cagaaaaagt agattcgact
    30361 tctttggata aagcatcgcc aatacctgcc cataagtatt tatcaggtgc ttcggcaata
    30421 attgctgtat caatgaaggt gtgtacaggt ggttttgaag gaaagaagag tccgtccatt
    30481 acgccttctt cgttatagat gacacatacc gcagtgacgg gcgaacagtt cgaaccaata
    30541 gtgggaaagg tgaagactgg tttagctagt ttttcaccaa tgacttttac agtatcgcaa
    30601 gcacggccac caccgactgc aaacaggacg tctgcttttt ggacactttc atgctggatc
    30661 aactgttcaa tatttccata agttgaatta cccccgtacc agagactggc gacaacctct
    30721 atttcctgtg tcaaagctac ttttattttg ttttctgcct ttgaaagtgc agtcttccct
    30781 ccaatcagca ctacttttgt cccatattct ttgacgattc catttgcttt ttcatacgca
    30841 tctgttccaa tggtataatt tggtaagaaa acactttgac tcatattcat ttccttcttt
    30901 cttcgttgaa acgggttaaa attttaaaga gtgatcgatc tgactgcggc tgtagatgga
    30961 gtagaggtga acctaagaca ggataagtga tgaataaggt ggcatgaaca ccttgaaatc
    31021 atcaataagt atcctaaaaa cgaacaagta caaaaacatt gaaaacgaca ataatgaatt
    31081 tttgttcctc ttagtatttc taccgattcc ctttgctttt ttgtaattat ctaaaaattc
    31141 taaaaattaa gattattata tgaaaacagg ttttatttgt caattcataa ttggtttttt
    31201 ttaaagcaaa agataatcta tatcatgatg aacaaacaga gttttttcct ccaattgatg
    31261 gctttcctga tctttcctat tttgaataaa gtgtttctgt ataatttttt tattttataa
    31321 gtaagataaa taatgaaaga atagagatac tgattggtag ctaaagattt ttgagcgaga
    31381 acatttcgtt ttttcaaaag gactgttaga ataagcaaga acaaggggga agacgtatga
    31441 taatcagtga atttgaccgt aataatccag tattgaaaga tcagctttct gatttactga
    31501 gactgacttg gccggaagaa tatggagaca gctcggcaga agaagtagaa gaaatgatga
    31561 atccagaacg aatcgcggta gcagcggtag accaagatga gttagtagga tttattggtg
    31621 caatccctca atacggtatc acaggttggg aattgcatcc attagttgta gaaagctccc
    31681 gacgaaagaa ccaaataggt actcgattag tcaattactt agaaaaagaa gtagcttcca
    31741 gaggaggaat cacgatttat ttaggtacgg atgatttaga ccatggaaca acgttaagtc
    31801 aaacggacct gtatgagcat acatttgata aagtggcttc catccagaac cttcgtgaac
    31861 atccgtatga attctatgaa aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa
    31921 atggctggga caaaccggat atttggatgg caaaaacgat tattcctcga ccaaattctc
    31981 aataataaaa agaggttgtg acagaagtaa tcagcttcaa gaaataagaa ggaatttacg
    32041 aaaattactt cttgaatttt tgtgaaattc caacttattt ccgaagaagc tacttttgga
    32101 acaccgtgta ccagaggttg tgaaaaagtc gcttagtttc gagtagtaag aaaaattttg
    32161 tgaaaatggc tttctacatt tttgcataaa tttggcttaa taccgagaaa ctggcttttg
    32221 aacaccgttt attcagataa gggactgtga caagactttt gtcacagttt ctttttgttt
    32281 attctttttt atctttgtat ggcaataata gacaatataa cgaaaattat ttaatggctt
    32341 ttggaagacc atggaaaacc ttgtacaaag ctattttttc tgaaaaattc cttgaaacct
    32401 cacgaaaaaa tcattatttt atgaaaaaga caaaaaaaag tgttgatttt ttaataatta
    32461 ttcattaaac tcttaaacaa cttcagaaaa tattaattat tctgaaaatt ccaataaagg
    32521 ggtacgtaaa atgaaaaaga aatttacatt tggtttggtt gcggtatgcg gccttgtact
    32581 tgctggatgt tatggcggca gttcagccac aagcagtagt tcagcaaaaa gcggttcagc
    32641 agatggagga ggcgtattta acttagtcgt gcctcaagaa atgcctacag ctgacttatc
    32701 agttgcaaca gatacaatca gttttacagc attgaataac gtttatgaag gaatttaccg
    32761 tcttgatgaa gacagcaaac cacagccggc aggagctagc gagttggcag aagtcagtga
    32821 agacggatta acttacaaaa tcaaattacg agaagacgca aaatggtcaa atggtgatcc
    32881 tgttacagca gcggattacg tttatggctg gcaacgaaca gtagacccag ctacagcttc
    32941 tgaatatgct tacctgtatg cgccagttga aaatgcagaa gacattacag ctggaaagaa
    33001 agataaatca gaactaggga tcaaagcagt tggtgactat gaattagaaa tcaaattgac
    33061 gaaacaaaca ccatatttcc aatatctgct tgctttccca tcatttttcc cacaaaaaca
    33121 atccgtagtg gaagaaaatg gggatgccta tgcatctgca agtgataaag ctgtttataa
    33181 tggaccattc acattagaag attttgatgg accaggaaca gatacagaat ggacttataa
    33241 gaaaaatgat cattactggg acaaagatac agtaaaactt tctgaaatca aagtcagtgt
    33301 agtaaaagaa tcttctactg cattgaactt attcaaagat ggccaagcag atgacgtgat
    33361 cttgtctggt gagttagcac agcaaaacgc aaacgatcca gcttatactt ctgttaaaga
    33421 agcgcggaca agctatatcg aattcaatca acgtgaaaag aattcaccat tcaacaatgt
    33481 gaacctaaga aaagctattt cttattctat cgatcgtgaa gctttagtaa aacaagtact
    33541 aggtgacgga tcagttgtct ctacaggatt gatcccatca gatatggtaa aaagtccaac
    33601 aacgaacgaa gactttgcaa aagaagctgg aaaattagtt tcttatgaca aagataaagc
    33661 aaaagaatat tgggaaaaag cgaaaaaaga attgggcgtt gattctttag aatttgattt
    33721 gatggcttct gatgatgatt catcgaaaaa agtaatcgaa tatgttcaaa actcaatcca
    33781 agaaaacttg gatggcgtaa cagtcaaacc aacacctgtt ccattctctg tacgtcttga
    33841 ccgctcaact tcgggtgatt ttgacgcagt actaggcgga tgggctgctg actatgcaga
    33901 cccaagcagc tttactgatt tgtttgtcac aggtaattca tacaaccgtg gtcgctggag
    33961 caatgcagaa tatgacgaag cagttaaagc agcttcatca acagacgctg gcgatgaaca
    34021 agcccgttgg gatgatttcc aaaaagcaat caagatcatc tctgatgata tgggtgtcgt
    34081 tccggtgtac caaaaagcag aaggtcacct agtgaatgaa aaaatcaaag gtatcgttca
    34141 ccatgcggca ggcgcttcat gggattacaa atggacatac gttgaagaat aatcgttaat
    34201 accgtgagca tgagaaaaaa cgaggctgcg gcaaatgtcg caacctcgtt ctcatattca
    34261 cttatcctaa caaagaaaaa agcatgaaaa tagcagaaag agcaattagt attccggcaa
    34321 tgatcagcca aaagctgtac ataccgtagc tggcagacga gagcgaggaa aattctggtt
    34381 ttttctttcg gttccttgtt cgtgcaagat ggaccgatcg ctgaaaatga tcgaagaaac
    34441 ccgaagaaaa gacccataaa aaaccgccga taatcagaaa cggcaatgac cataagaata
    34501 gatcattaga taattgaatg agtgtaaagg aatggttgat gagatttttt attaaaataa
    34561 ccagaatcag tagaaaagct actgcgtagg ggatagattt ttttttcatt tgattgctcc
    34621 tttatttttg gttcattatc taataatgta gcgaaacatc tgtcagaagt caaaagctag
    34681 ggggaaacat tagtgaatag ttatatcaaa tatgtattaa aacgtgtctt tttcatgatc
    34741 atcacgcttt ggttaattgc aacaatcacc tttttcttga tgcaattgct gccgggcacg
    34801 ccatatacga atcaggaacg tttgagtcct gaaacaatcg agatgctaaa taaacaagtc
    34861 ggtttggata aaccagttat cgtacaatat gggatttatc tttctaattt gcttcaagga
    34921 gattttggaa tttccttcca atttaaaaac cagccagtcg cacatttgtt agctggaaga
    34981 atcggaccat ccttgcagtt agggttacag gcaattatct ttggaacagt cctagggacg
    35041 attttaggaa cgatctctgc catgaagcaa aatacatggg cagatacatc ttctacactc
    35101 gtagcgatat taggtcgttc cattccaaac tttgtatttg ctgttttatt acaatatatc
    35161 tttgcaatca aattacaagt tttgccaatc gcaaaatggg atggctttgt gtacacaatc
    35221 ttaccaacaa ttgcgttggc catgtcgccg cttgctgact ccgctcggtt tatccgtacg
    35281 gaaatggtgg aagtactgca tagtgattat gtggaattag caaaagcaaa aggattgagc
    35341 cgttgggaaa tcgcctttaa acatggtctt cgaaatagtt tgattccatt gatgacttta
    35401 ttaggaccgt tagctgtagc attgatgacc ggatcactgg tggtagaaaa tatctttgct
    35461 attcctggta tcggggaaca attcgttaag tcaatcacaa caaatgatta tcctacgatc
    35521 atggctgtaa cgatccttta ctcatttatg ctgatttttg ttattttgat cgtggatcta
    35581 ttgtacggtc tagttgatcc aagaattcgt gtttcagaag ggagccgagg ataaatggaa
    35641 atgataccaa agcgttatga aacagttgca gatatccctg cggatgaatt tcttccatta
    35701 caaaaaaata ccgaagaaga acgtgaacag attactgcac cttctttgaa ttttatccaa
    35761 gattcatggc gccgtttaaa gaaaaacaaa gctgccgtta tttcaatggt tttactattg
    35821 gtcatcattt tgatctctgt gatcacaatc tttgtttctc ctcatgatcc aactgcccaa
    35881 aacgtggctt atatcaattt gcctcctcgt attccgggaa tcaatatcga tggattgaat
    35941 ggaaaagcaa tggttggtgg tgaacttgtt gataaatatg ctcaagcaaa cgtcccttca
    36001 aacgtaaatt tctttttagg aacagacggt ttgggacgtg atgtattaag ccgcttattt
    36061 atgggaatga gaatctcgtt gctgatcgca tttattgctg cattactaga tgtaacgatt
    36121 ggtgtagctt acgggttggt ttctggtttg ttaggcggac gtgtcgataa cgtgatgcaa
    36181 cgttttttgg aaatcttatc aggaattcca aacctcgttg tcatgatttt gatgttggtc
    36241 gtttttgaac caggaatttt ttcaatcgtg gctgctatgg ccatcacaaa ctggatacca
    36301 atggcacgta tcgttcgtgc acaaacaatg aaattaaaag atcaagaata tgttttagct
    36361 ggattgacat taggtgaatc aagaatgaaa attgcattca aacatatttt gccgaatatc
    36421 tctagtgtca ttattattca aatgatgttt agtattccat ctgctatttt cttcgaagcc
    36481 tttttaagct ttatcggttt aggtcttacg cctccgtcag cttcattagg aacgatgttg
    36541 agcgaagggt acaagacatt cctgtatctg ccttatctat tatggattcc ggcagtaact
    36601 ttatcggtta tcatgattgg ctttaactta cttgctgatg gattgcgtga tgcctttgat
    36661 cctaaaatga aagagtgaag acgatgacga aaattttaga agtgaaagac ttagaaatct
    36721 cttttgatac gtatgctgga aaggtacgag cgattcgcgg tgtcaatttt catttgaata
    36781 aaggagaaac attggcgatc gttggggaat ctggaagtgg aaaatcagtg acgaccagaa
    36841 gcatcatgcg cttgctgtca agcaacgcca atatagatgc tggtcaaatc ctctttaaag
    36901 gtaacgatat cgttggaaaa tcagaaagag agatgcaaaa aatccgtggt aaagaaatag
    36961 cgatgatttt ccaagatcca atgacctctt tagatccgac gatgcccatt gggaagcaag
    37021 tcgctgagtc ccttagaaag cataataaag tttcaaaaaa agagggacaa aaagctgcat
    37081 tagaattgct taaattagtt ggaatacccg aagctgagaa acgtatcaac agttatccgc
    37141 atcagttctc aggaggtcag cgtcaacgga tcgtgatcgc gattgcactg gtatgttatc
    37201 cggaaatcct gattgctgat gaacctacaa cagcgcttga tgtgactatt caagcacaga
    37261 ttttggagct gctgaaagaa attcagacaa aaatcgatac atcaattatc tttatcactc
    37321 atgaccttgg tgtagtagcg aatgtcgctg atcgtgtcgc tgtcatgtat ggaggaagaa
    37381 tcgttgaagt tggaacatcg gaagaaattt tctacaaccc gcaacatccc tatacttggg
    37441 gactgcttgg ttcaatgccg acattagata gtgcgaacga tcgattgtat gctattcctg
    37501 gttctccacc ggatttattg aacccgccaa aaggagacgc attctatcca agaaatgaat
    37561 tcgcgatgaa aatcgatgca gaacaagaac caccattttt tgaactatca aaaacacaca
    37621 aggcagcaac ttggctactt gctccgcagg cacccaaagt gacaccgcca gaagaaatcc
    37681 aacgtcgctg ggctatcttt aaagagaaac aaaaacatac ttacggtcaa ggaggaattg
    37741 ctgatgccaa ataaactact tgaagttaaa ggattgaaac agtatttcaa tgtaggaaaa
    37801 aagaatgaag tacatgctgt aaatgacatc agctttcaca tttatgaagg agaaacattc
    37861 ggtttagttg gagaatctgg aagcggaaaa tccactactg gccgtacgat cattcgcttg
    37921 aatgaaccta ctgatggtga gatattattc gacggtcaag atataacgaa gatcaaagat
    37981 aaaaaaggtt tgacgaaatt ccggcatgat gttcagatga tctttcaaga tccatacgct
    38041 tcgttgaatc ctcgtatgaa agtcagagat atcattgccg aaggaattga tgttaatgga
    38101 ctagctaaat catctgaaga gcgtgcaaaa aaagtcgatg atctattgag aactgttgga
    38161 ttgaatccaa gccacggtac tcgttatcct catgaatttt caggtgggca acgccagcgt
    38221 attggtattg cacgagcatt ggcagtaaaa ccgcgtttta tcatttgtga tgaaccaatt
    38281 tctgcactag atgtgtcgat ccaagcccaa gtggtcaatt tgttgcaaga tctccaaaaa
    38341 gaacatcaat tgacctatct atttattgca catgatttgt cgatggttaa acatattagt
    38401 gaccgaatcg gtgtaatgca taacggcttg ttattagaga tgggaacaag tgatgaaatc
    38461 tataatcacg gtgttcatcc ttatacagaa agtttgttat ctgctattcc tctaccagat
    38521 ccagaccatg agagaaaacg cagaagaatc aaatatcaac ctgaaccaga tgacggacaa
    38581 gtacgtcaac ttcgtgaaat cgcaccagaa cattttgtct atgcaactga gcaggaagtc
    38641 ccatattacg caaaaaaact aaaacgacaa aaggaatctc ttctggttgc taactaggag
    38701 ggacatagat gctaaaagct tttaaattcc gtatgtatcc gacagaagaa caaaaacagc
    38761 aattgattcg tacttttgga tgtgctcgat ttacctataa ccatttgctg aaaaagcggc
    38821 agaaaagttg gcaacaaaca ggagtagcca atttttcgct cacacccgct acattaaaaa
    38881 aagaataccc attcttaaaa gaagtagaca gtcttgcttt agcaaatgca cagcttaatt
    38941 tggatcgggc ttttcgcaat tatttcaagg gacgtgccag cttcccaaag cttaaaacga
    39001 aaaaaagtat gtggcaatct tatacgacca acaaccaaac ccgtacgatc tatctggaaa
    39061 atggctactt aaagttacca aaacaaaaag aactaatcaa gataaatcag catcggccag
    39121 ttgaaggatc gattcgctcc gctacgattt ctgctcgata taatgaagaa ttttatgtcg
    39181 cactactttg tgatgtttcg ccagtaaaaa aagaatcttt agctaaatgg atcgggatcg
    39241 cctatcatcc gaagacattg atccaaacat ctcgacctct agaagtaact ttaccaaaat
    39301 ttcaccagac agaagaaaag ctgcaacatg cacagcggaa attgaatgtg aaagtccgct
    39361 ccgctcacca tagaaagatc aggttagatc aggcaagcaa ttatcagaaa caaaagagaa
    39421 aagtaatgga tctttatctg aaacaaaaga accagcgaga agattatctt gaacaattat
    39481 ctgggaaatt ggtcaaacaa tatgactact tatttgtaga atcttttcca aaagaagagg
    39541 cacatgctga tttttctatt cacgactggc ataaattaat aacaaaattg cgttataagt
    39601 cccaatggta taataaaaaa tttcttttga tcaataccga cggagcagaa gaaagcaatt
    39661 ccgtaagaaa gagccaagta gtagaaaaaa tgggcagaca ttcggttatc aagggataaa
    39721 aaatgaatga aactgatagg agcgttaggg ttagccttgt taataaaaag tcttaatgac
    39781 ttttgtttct aggaattttt cttttgaaga aaagctcact aattatgaca aaccaataaa
    39841 acaatagcag aaaaagttcg tacgagcttt ttctgctatt gttaattttt tattcagaaa
    39901 gtttacgaat acatcaaaat tctgaaacaa aaaacgtaac tcacaaagag ttgtttttgc
    39961 gttttttctt gaatgattag gtctgaatct tttgctcaca accttattca tggtgccccg
    40021 gcttgagctg atagattttt gaaggacggc cgattcctac aggacgttca ccgatctcat
    40081 cgaaatattg gagcatagct ttcttaaaat tggaatgatc gatcgtttta taatcgatcc
    40141 ctaagaattt agcaaatact tttcgagctt ccgtaatcgt gaaatcttta cctaaaactt
    40201 gtagaacttg cggctcgtgt tccattttat tgactacacg gttgaatgct ttcaagatga
    40261 tttgactatg gtcaaaagcc aaagtatctt tgccagaaga ctctccagtg actaaatcaa
    40321 gcgagatagc cacttctccg ctagataaat ggatcttgtt actatgacgt tctagtgtga
    40381 accagcgtac ttccttggca tcatctccgg cgattagcgg ttcttctcca ataaaggcta
    40441 agtagctgac tgtgacgacc cagcctcgcg gatctcgatt aggcgtactg aatgtatgca
    40501 gttgctcaat attttctttt gaaataacca caccagtttc ttcctttgtt tctcttaaga
    40561 cgctttctcc agttgactca tccttttgga caaagccacc aggcaatgcc catgaatttc
    40621 gataaggatg cccttttctt tggatcaaca aaactttcag ctgatcttct tctttgttat
    40681 agcacatcag tacgatatcg acagtcaaag agggcttgtc atattctgga cgttcctgtt
    40741 tttgatacca gttaagaaat tcttcttcac ttgctttatg ttcataatat aatttttctt
    40801 cttctttaga agcaaaacgg ttcatctgac cacctccatt gacagtaaaa gtacttttta
    40861 ccttattgta gcagactctg aaaaaaaacc aaggatttac ttgaaaaatc taggttatca
    40921 tttaaatatg aaaaataaat ggaagtatga gattgaataa aaagaaaagc agataaaacg
    40981 gcaatacttt ataccgctct tcacgaatgg caaagaagcc acaaataacg atatatacgt
    41041 caatgataaa acgcaaaatc caagctgctg tcataaaact gttcaaatgc ccatggaggt
    41101 taaaataacc atacatacga atataaaaat agctgaatat taaatgtgct aatgaaagaa
    41161 aagcaatact aagtgtaaaa aaaagatgtt tcttctgcat gattgttcca gtcccttact
    41221 aagatagata gtctttatta taccacgtgt gaggggagaa ataaaaagcc gttttttagt
    41281 agatatgaaa caaaacaaaa acccgcattt attcaaccta ttcatccttt taaaggatag
    41341 attgaacgta gatagaagtc gtttatccag tctttttcat tttgaaaaaa agctaacagc
    41401 atgctttttt tatggtatac tagcaaggaa acgaattcgc atcatgcgat aaaggaggaa
    41461 gagccccgtg gcagaaaagg aaacttttta tatcactacg ccaatctatt atccaagcgg
    41521 caaacttcat atcggcaact cgtatactac gattgcatgt gacgcaattg ctcgttataa
    41581 acggttgatg ggctttgatg tgttttatct cactggagtg gatgaacatg gtcaaaaaat
    41641 cgaaacaaaa gcagaagaac taggtgtaaa accgcaagaa tatgtagata aaatggcggc
    41701 tgatgtcaag aaattatgga aaacgcttga tatcagttat gataaattca ttcgcacgac
    41761 agatgattac cataaagcag ctgttcaaaa aatatttgat cgtttattag aacagggaga
    41821 tatttactta ggtgaatatg aaggctggta ctctgtttct gacgaagaat tctttacaga
    41881 aacacaactt gcagaagtgt acaaagatga cgaaggtaac gtgatcggtg gtaaagcgcc
    41941 aagtggacac gaagtagaat tggtaaaaga agaatcatac ttcttccgca tgagcaaata
    42001 cgcagaccgt ctattggaat actatgaaga acatcccgaa ttcatccagc cagaatctcg
    42061 taaaaacgaa atgatcaaca actttatcaa accaggatta gaggatcttg cagtttcacg
    42121 aacaactttt acttggggag taccagtcaa gagtgatccg aaacatgtgg tgtatgtatg
    42181 gatcgatgca ttatccaatt atattactgc actaggatat ggctccgaag atgaaagtct
    42241 attcgaaaaa tactggcctg cagatgttca gatggtcggt aaagaaatcg ttcgctttca
    42301 tacgatctat tggccaatca tgctgatggc ccttgatttg cctctaccta aaaaagtatt
    42361 tgggcatggc tggctattga tgaaagacgg aaaaatgtct aaatcaaaag gaaatgtcgt
    42421 ttatcctgaa atgcttgtgg aacgttatgg tttagatgca ctccgttatt atttgcttcg
    42481 tgctgttccg tttgggtcag atggtgtctt tacgccagaa gattttgttt cccgtgtcaa
    42541 ttatgatctg gcaaatgatc tagggaactt attgaatcga acgattgcta tgatcaacaa
    42601 atattgtgat ggactcgttc ctgactatgc ctcacttgtc actccatttg atagtgaact
    42661 gtctacgacg gctgctaacg ttgtcggacg ttaccatgat gcaatggaaa aaatggaatt
    42721 caatacagca ttggctgaaa tctgggtgct gatttctcgt gcaaacaaat acattgatga
    42781 aacagaacca tgggtattag caaaagacga agaaaagaaa gctgaattga atagtgtcat
    42841 gatccattta gctgaatctt tgcgtatcgc agcgatatta cttcagccaa tcatgacaga
    42901 aacgccagag aagatcttca atcaactagg attggactct gaaacaatga atcttgaagg
    42961 attacacttt ggcgaatttc cttcaggaac aaaagtagtt gcaaaaggta caccaatctt
    43021 cccacgttta gatatggaag aagaagttgc ttttattcaa gaaaaaatgt ctgaaggtac
    43081 tcaaacaaac gaagatacag tcaaatggga tccagaagaa acagaacttg tttcaacgaa
    43141 agaaaaacaa atcaaatttg atgtatttga aaaagtagaa ctaaaagtag cagaagtcat
    43201 caactgtcaa aaagtagaag gggcagataa gttgctgcaa ttccgactag atgctggaga
    43261 cagccaagac cgtcaaatct tgtcaggaat cgctgaattt tatcctgatc caagcgaatt
    43321 gatcgggaaa aaagtcgtca ttgtagcaaa tctgaaacca cgaaagatgc gtggacaaat
    43381 cagtcaagga atgatccttt ctgcagaagc accagatggc tctttacaag taatcgaagc
    43441 accaaaatcc atgccaaatg gctcagaaat cgcatagaaa ttcaaagggg gtatgacact
    43501 agttgtgtca tacccccttt ttccgaaatt tgagaagcaa ttttcgtaaa ttccttctta
    43561 ttacttgaag ccaatcatat aatacagaaa cagagaacta tccaaaataa atcctagaaa
    43621 aagaagtacg agcttcgctg atttttgtta atttagaata atactaatag gaatcgtaaa
    43681 aacagaaaaa aagcataatg ataaagggtc gggcagattt taattgattc atctggtact
    43741 cttttttatt gtccttacat tcataaagtt tgaatgcaat gactttatta catctatatt
    43801 tatatctaac agtagcgttg atgtgttaat tataagttct atcactttat gttcaattag
    43861 tttgtgctct tatatgtgtg attgaaaata tagataaaaa aacaatcatc ggctttgact
    43921 tcgatgattg ttttctttat tttgtaaaaa tcctatcact tgattttatg gtaacctttt
    43981 atttttgtct tcaaacggct tggtaagaga gcagtaagca gtgcaaatgg gatcgaaaag
    44041 acataccaga aaatcgtacg tccgatacca taatgttgat agagctttgt caacatcacg
    44101 tgtaacaaga tcaaccctaa aagcaaggag ccagctataa accaatgatt atcccaatgc
    44161 atgacttcac taagtgccat cactccaaaa ggatagaaat aaaaatagat cccgactaag
    44221 gtcatccgcc agttctttgt aaatgttccg atattcaata aaaagaaaat cgctgaaagc
    44281 aaaataccga taggcggaaa aagcccaacc aaaaaggaat acaacatccc ccacaagatc
    44341 atcacaggac ctttgatcaa tgcaacgatt gcgatgaaca aagtcagcaa tattagtttt
    44401 tgtgataacg ttgcaacagt cattaatcca aataaaatta atgtgaacca atgccaatgg
    44461 gactgctgtt gttctttacg ggaaaaatcg acccggttgg ataaataatc tgctaatttc
    44521 ggataagaac gcttaaaatc cataaactgt tcctccttcg tggaaaaggt aggtttagta
    44581 tacgaaaaat aaaaaagaaa tacatccgtc ttaagaggga gtttttcact gaaagtggag
    44641 aaaaaagaat ggtaaattaa aatattctca gttatactga aaattatacc aaatttcaaa
    44701 gaagaaagag gatgggaatg aaaggcaaac taaaacgaga aatcaatctg tttggcgcat
    44761 tagctaccgt tatgggaacc gtgatcggtg caggcgtctt cttcaaaact gctgctgtta
    44821 ctgctagtac tcagtctaca agtttgacat tgctagcttg gcttttgggt ggtttcttaa
    44881 cgatctgtgc tggattgaca gtggctgaat tagctacagc aatcccggaa actggtggag
    44941 ctgtaaaata tattgaagct gcgtatggaa aattgcccag ctttttatta ggatgggcgc
    45001 aaagcttgat ttactttcct gctaatattg ctgcattatc gattatcttt gcgacacaat
    45061 tgacgaactt attacaatta tctaccgatt atcttttgtt gattgcagtg atcaccgctg
    45121 tttctgtgac agggttgaat ttgttaggga caaaagtcgg tgcttctgta caatcagtga
    45181 cgttgattgt taaattaata ccaattgctg tgattgtcat atggggatta ttgaccccag
    45241 gacaaggaac tgtccagtta tttccaatcg aagctggaaa agatgttact tttgtcgaag
    45301 gattgagcag tgcgttgtta gcaacgttgt ttgcttatga cggctggtta ggtgtcggtg
    45361 caatggcagg ggaaatgaaa cggccggaaa aagatttgcc taaagcaatt attcttggtt
    45421 tgagttttgt aacagtcgtt tatctattga tcaattttgt ttttttgaaa actttgccga
    45481 ttgatcatct tgccggtaat ttgaatgcag cttctgaagc atcagacgtc atttttggag
    45541 gaatcggtgg aaagctggtc actattggga tcttgatctc ggtatacggt gcattgaacg
    45601 gttatacact aactgggatc cgcgtgcctt atgcgatggc tttagaagac gagctacctt
    45661 tcagtaaaca gctgacaaag ctttcaaaga agtttacggt tccttatgtt cctgcggttt
    45721 tccagttagc agtagcttgt attatgatga gccttggttc tttcgacttt ttaacagaca
    45781 tgttgatttt tgtgatgtgg ctattcagtt tactgatttt tatcggtgtc tttattttaa
    45841 gaaaaaaagc gccggaattg ccacgtccat ataaagtacc actttatcca atcgttccga
    45901 ttattgcgat tctcggtgct attttcattt taggtatgac gatgatgacg caaacgaaat
    45961 tagcattgat cggtataggt gttaccttga taggtatccc agtctattat caaaaaaaga
    46021 aaaaacgctc agaagagtaa aatatttcaa caacagacag aaaaagtacg aactaggtcc
    46081 tctagatctt cgtgcttttt ctatttcaat aaaacaagtt cttctatttt gatttttgct
    46141 acttctgcaa gtagtgctta tgtgctaaag taagaatgaa aaggagagat aaacatgata
    46201 tttgattctc atacccattt aaatgctgaa cagttcaatg aagatattcc agaaacgatt
    46261 cagcgagcac aagaaatggg ggtaacgaaa atggcggtcg tgggcttcga tacgccaacg
    46321 attgaaaagt ctttgcaatt aagccatgac tatccaaata tatacagtat tatcggctgg
    46381 catccgacag aggctggcag ttatacgaaa gaaatcgaaa ataaattaca agaacaattg
    46441 actttaccaa aggttgtagc attaggggaa attggcttgg attatcattg gatggaagac
    46501 ccgaaagaag tccaagataa agtatttcgc agacagattg ctatcgcaaa agaaatgaac
    46561 ctaccaatca gtattcatac tagagaagca cttgaagaca cgtatcaaat cctaaaagaa
    46621 gaagatgttc gagatatcgg tggaatcatg catagtttca gtggtgatta tgaatgggca
    46681 aaacgatttc ttgacttagg gatgcatatt tcatttagcg gtgtcgtcac tttcaaaaaa
    46741 gcattagacg tccaagaggc tgcagcaaat gtaccaatcg aacgattact tgtggaaaca
    46801 gatgcccctt atctggcacc agttcctttt agaggaaagc gaaatgaacc aggctatact
    46861 cgttatacag tagaaaagat tgctgaactt cggaatcttc cgacagaaga agtggctgcc
    46921 caaacatgga caaatgcgca ccgattattt ggtctaagcg aacatggata agatgaagat
    46981 ccaagagatt atagtcgttg aaggaaaaga cgatacaaga aggctgcggg aagtagttga
    47041 tgcagatacg atagaaacaa tagggtctgc aatcaatgaa gagatcctga tgcaaattga
    47101 acatgcccaa gagactcgtg gagtgatcgt ttttaccgat cctgattttt caggagaaaa
    47161 aataagaaaa atcattatgg aagctgttcc tcaggcaaaa catgcttttt tgccgcgcac
    47221 acaagctaaa ccaaagaaaa agggaagcct tggtgtcgag catgcaagtg atgctgctat
    47281 tttagaagca ttgaaaaagg tcgtgacgcc agacatttcc caaacggctg tttctgaaat
    47341 cactagacaa gaccttttag actatggttt aatagctggg gcacaagcaa aagaaaaacg
    47401 agaaaaacta ggtgacgagt tgcgcatcgg ttacacgaac ggcaaacaat tggaaaaacg
    47461 tttgaagatg tttcgtatta cgccgaaaga actcgcccaa gcaatgaaaa tagtggagga
    47521 acaacatgag tgaatatcgt gaaattgcga caccctctag aacaaaagag attttattga
    47581 aacatggttt ttcatttaaa aaaagtttag gacaaaattt tctgactgaa ccaaatattt
    47641 taaggaaaat cgtagaaact gcgcaaatag atgaacatac gaacgtgatc gaagttggac
    47701 caggaatcgg tgcattgacg gaacaattag ccaagcatgc aaaacaagtc gtagcatttg
    47761 agatcgatga tcgtttgatc cctgtattgg ctgatacgat gcaaccctat gacaatgtga
    47821 ctattgtaca tcaagatatt ttaaagaccg atttgtcaac agctgttaga gaaacatttc
    47881 atgaggagct tccgttaaaa gtcgtagcga atttacctta ctatattacg acaccgatca
    47941 tgatgcattt tcttgaatca gatttggttg tggatgaact agtcgtcatg atgcaaaaag
    48001 aagtagctga tcgtatctct gctgaaccag gaacgaaagc ttatggttca ttatcgattg
    48061 ctgttcaata ttatatggaa gccagtttag cattcatcgt accaaaaaca gtatttgtcc
    48121 ctcagccaaa tgtagattca gcgattttga aactgacaag aagagatata ccggcagtcg
    48181 aagtgacaga cgaaaaagca tttttccgat tgacaaaagc ggcatttcag caaagaagaa
    48241 aaacgctttg gaataacttg cagcattcgt atggcaaaga tgaccagaca aaagcatggt
    48301 tagcaaaaag cttggaaaca gctggaatcg atccaaaacg tcgtggggag actttgtcat
    48361 tacaagaatt cgccgcatta agcaatgcga tgtcagaaaa tatgcagtaa aaaaagaggt
    48421 tgtgacagaa gttgtgaaaa agctgtcctg catcaagtaa taagaacagc caaacaaaaa
    48481 tagttcctca tgtttttcgt ttgtttgggc ttattaccgc agatgcaagc ttttgaacac
    48541 cgtttaagaa agaggttgtg ggcctgccgt ttaactctaa gtcacaagga acaatttcaa
    48601 aaaacagctt ctcatgtttt tgagttagtt ggtcttatta cttgatgcaa gcttttgacc
    48661 actattatgc cgaacaagga gatgtgacac aaattttgtc acatctccta tttttgtttc
    48721 ttgatcaaaa aattcttata cttgatactc acactcattt tgtatggatt tagaaaagaa
    48781 tatgtgtctt gacagttatt agtaagtgag ttatactaag tcgacattta tgtgtcaaga
    48841 catattagga gagagaaaaa atgagaaaaa attcagttcg aaaactaacg atttctgctt
    48901 tattgattgg gatgggggtc attatcccaa tggtcatgcc taaaatcatg attccaccag
    48961 cttcctttac tttggcgtct catgtgccat tatttattgc gatgtttttt acaccaggag
    49021 ttgcagtggc tgttgcgcta ggtacgacat ttggtttctt tttaacaacg ccggttatca
    49081 tcgcattgcg agcattgtca catcttgtgt ttgcgttgat tggggcttgg tacttgcaaa
    49141 agcatccgag tatcgtttta aaaaatggac agtttacttt tgctaattgg cgtttccaag
    49201 ggttcaattt tgtgatcggt gtgattcatt ctttagcaga aatgctagta gtcagcatct
    49261 tttactttat tggtaatatg cctgaaacat attattctca aggatatttt tatacagtat
    49321 tcatcttgat ggggattggt gggcttatcc acagtttagt tgattataat attgcttatt
    49381 ttatagcagg aacactcagc aaatattttg atatccccgt atttacggca gcaaaaaaag
    49441 aaaatcggaa agagtcgatt caagggtacg cgaagaaaac gacatattag acatgatctc
    49501 tcaaaaaaaa aactctcatc tagtaatgga taataaatcc tctaagatga gagttttttg
    49561 tcctaaaaag agctgcacca acatcttctg gcacagctct aacagggggc aatttatgga
    49621 agtttgatga cttgttctgt ttctttatca aagaagtggc ctttgctgat gttgaaggca
    49681 aggtcgacta tttcgcctgg tttatggaaa tctcttgcat ctacttttga gatgaactca
    49741 gtagaaccag tttttgtgta aagcatcgtt tcagctccaa gtaattcgga aacgacaact
    49801 tctgatttca ctacggcttc aggtgaagct tcaagggcta cttgttcact gtggatgtct
    49861 tctggacgaa taccgaagat aacttctttt ccttcataac ctttttcaac taatactttg
    49921 ttttttcctt caggaatacg aagcttaagc ccatgtccat ctgtgataac accatttttc
    49981 aatgtcacat taaagaagtt catggcagga gatccaataa agccggcaac gaacatattg
    50041 acgggtgtat cataaacttc tttcggtgta ccgatttgtt ggataaatcc gtctttcatg
    50101 ataacgattc ggtcagccat agtcattgct tctgtctgat cgtgagtaac atagatggtc
    50161 gttgtttcaa gacgctggtg aagtttggca atctcagcac gcatggctac acgtaattta
    50221 gcatctaaat ttgataatgg ctcatccatt aagaagactt ttgcatcccg aacgattgcc
    50281 cgtcccaatg ccacacgttg gcgttgtcca ccagataaag cagctggttt tcgtttcaag
    50341 tattctgtca gacctaagat ctctgctgca ttgtccacac gttttttgat gtctgcttta
    50401 tcatatttac gtaatttcaa gccaaaagcc atattatcaa aaacagtcat atgaggatac
    50461 aatgcgtagt tttggaaaac catggcaata tcacggtctt taggtgccac gtcattcatg
    50521 acttttccgc cgatatttag ttctccttca gagatatctt ccagtcccgc gatcattcgc
    50581 aaagtagttg actttccaca accggaagga ccaacaaaaa cgataaattc acggtctttg
    50641 attgatagat taaaatcagt tacagaataa ttttctgcat tgtcatattt tttatatacg
    50701 tttttcagag ccatttctac cataatacgt tccacacttt cttttttagt tttattaact
    50761 atttaaagta taaatgaaag cgttaactaa cttcaatgtc atgttgaaca agaatttttt
    50821 cttttttttg tcaatgtgct agttttaagc tttaataaat atccataaga aataaaaact
    50881 gatacactgg agtaaataag tatgttaaca atatcaaaag gagtgtctct tatgaaaatc
    50941 gctttgatcg ctcatgaccg aaaaaaacct atgatggtga aattagcaac agcgtataaa
    51001 acaatattgg aaaaacatga actttttgct accgggacga ctgggcagag gatcacggaa
    51061 gcaaccggct tgccgatcca tcggtttaaa tccggaccgt taggcgggga tcagcaaatt
    51121 ggagccatga tttcggagga tcagttagat ttggtgattt ttcttcgaga cccgctggct
    51181 gcacagcccc atgaaccaga tgtaacagca cttatccgtt tatctgatgt ctatgagatt
    51241 ccgttagcta cgaatatcgg aacagctgag gtgctactaa gaggactgga agccggcttc
    51301 ctagattttc gaagaatcgt ccatgaaata gacaagcatc cactttcatt ttgatggata
    51361 agcgtatgga aaaataacgt ttaatgttcg cctatatgta gctataaaag ctaaacaaac
    51421 tgataatatg cgatataata cgaacgtatt ggataatata aacaataata ttagtttttc
    51481 gtatttgcag taaatgtgaa aatatgctat agtttctgtg ggaaaagatc ccaaaaataa
    51541 tacagagtag aaaataaagc gttaagtgct aagaggatgg gatgttgcct cttggacgaa
    51601 ggacgtaatg ttcgcggttt tattttcgca ttcgctgcat gatacgatgt agcagctctt
    51661 aaaggagatg ctaaaaatga agaaacaccg gttagacgca cgtatgattg ctattatggg
    51721 cttattgatt gcattgatgg tcactttgtc ccgactggta gcaatcgaga ccccatttat
    51781 taaaatcagt gtcacattta ttccacaagt gatcatgggc attctttttg gtcctttttg
    51841 gtcagggata ggcgcagttt tagctgattt agtaggaatg gccctcttct cgaaatctgc
    51901 attctttatt ggatttacat tgaatgcatt tattgaagga gcaatctacg gattcttttt
    51961 ctatcgaaaa gagatcactt ggaaaaatgc cattctggct acgcttagtg tcacgctgat
    52021 catcaactta tttttgacac cattatggct tgcgttgatg taccatgtgc cattgttcag
    52081 ctgggtcgtt tgggcgcccc gcctgttaaa aacagtaata tggttaccga ttcaatcaat
    52141 tgccatttat tacgtaggac gtagtattcc ttacaaaaaa attttaagaa gtttagcgat
    52201 ccacgctaaa taaa
//
LOCUS       DASHRQ010000014        52160 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.014, whole genome shotgun sequence.
ACCESSION   DASHRQ010000014 DASHRQ010000000
VERSION     DASHRQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.014"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            330..1175
                     /locus_tag="U2M88_000913"
     CDS             330..1175
                     /locus_tag="U2M88_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM8373310.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="U2M88_000914"
     CDS             1456..2676
                     /locus_tag="U2M88_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEM8373311.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="U2M88_000915"
     CDS             2673..5393
                     /locus_tag="U2M88_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM8373312.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="U2M88_000916"
     CDS             5377..6321
                     /locus_tag="U2M88_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEM8373313.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="U2M88_000917"
     CDS             complement(6390..7400)
                     /locus_tag="U2M88_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEM8373314.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="U2M88_000918"
     CDS             complement(7580..7924)
                     /locus_tag="U2M88_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEM8373315.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="U2M88_000919"
     CDS             complement(7917..8351)
                     /locus_tag="U2M88_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEM8373316.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="U2M88_000920"
     CDS             8489..9226
                     /locus_tag="U2M88_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8373317.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="U2M88_000921"
     CDS             9219..10430
                     /locus_tag="U2M88_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8373318.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10634..11416
                     /locus_tag="U2M88_000922"
     CDS             10634..11416
                     /locus_tag="U2M88_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEM8373319.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="U2M88_000923"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="U2M88_000923"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEM8373320.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="U2M88_000924"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="U2M88_000924"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEM8373321.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="tadA"
                     /locus_tag="U2M88_000925"
     CDS             14132..15061
                     /gene="tadA"
                     /locus_tag="U2M88_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEM8373322.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="U2M88_000926"
     CDS             15018..16067
                     /locus_tag="U2M88_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEM8373323.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="U2M88_000927"
     CDS             16064..16342
                     /locus_tag="U2M88_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEM8373324.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="U2M88_000928"
     CDS             16317..16787
                     /locus_tag="U2M88_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEM8373325.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="U2M88_000929"
     CDS             16804..17106
                     /locus_tag="U2M88_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373326.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="U2M88_000930"
     CDS             17123..17494
                     /locus_tag="U2M88_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEM8373327.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="U2M88_000931"
     CDS             17466..17825
                     /locus_tag="U2M88_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373328.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="U2M88_000932"
     CDS             17997..19004
                     /locus_tag="U2M88_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM8373329.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="U2M88_000933"
     CDS             19029..20213
                     /locus_tag="U2M88_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEM8373330.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="U2M88_000934"
     CDS             complement(20650..22233)
                     /locus_tag="U2M88_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEM8373331.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIDYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="U2M88_000935"
     CDS             complement(22811..22954)
                     /locus_tag="U2M88_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEM8373332.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23177..23656)
                     /locus_tag="U2M88_000936"
     CDS             complement(23177..23656)
                     /locus_tag="U2M88_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEM8373333.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23729..24013)
                     /locus_tag="U2M88_000937"
     CDS             complement(23729..24013)
                     /locus_tag="U2M88_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373334.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24091..24285)
                     /gene="ssrS"
                     /locus_tag="U2M88_000938"
     ncRNA           complement(24091..24285)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="U2M88_000938"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24357..25643)
                     /locus_tag="U2M88_000939"
     CDS             complement(24357..25643)
                     /locus_tag="U2M88_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEM8373335.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25781..26452
                     /locus_tag="U2M88_000940"
     CDS             25781..26452
                     /locus_tag="U2M88_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEM8373336.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGAIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26418..26906
                     /locus_tag="U2M88_000941"
     CDS             26418..26906
                     /locus_tag="U2M88_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEM8373337.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26919..27866
                     /gene="prmA"
                     /locus_tag="U2M88_000942"
     CDS             26919..27866
                     /gene="prmA"
                     /locus_tag="U2M88_000942"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEM8373338.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27867..28631
                     /locus_tag="U2M88_000943"
     CDS             27867..28631
                     /locus_tag="U2M88_000943"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEM8373339.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28864..31077
                     /locus_tag="U2M88_000944"
     CDS             28864..31077
                     /locus_tag="U2M88_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEM8373340.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31088..31534
                     /locus_tag="U2M88_000945"
     CDS             31088..31534
                     /locus_tag="U2M88_000945"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEM8373341.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31705..32964
                     /locus_tag="U2M88_000946"
     CDS             31705..32964
                     /locus_tag="U2M88_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEM8373342.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33053..34000)
                     /locus_tag="U2M88_000947"
     CDS             complement(33053..34000)
                     /locus_tag="U2M88_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEM8373343.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34394..35686
                     /locus_tag="U2M88_000948"
     CDS             34394..35686
                     /locus_tag="U2M88_000948"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEM8373344.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35701..37470
                     /gene="aspS"
                     /locus_tag="U2M88_000949"
     CDS             35701..37470
                     /gene="aspS"
                     /locus_tag="U2M88_000949"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEM8373345.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37579..38454
                     /locus_tag="U2M88_000950"
     CDS             37579..38454
                     /locus_tag="U2M88_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEM8373346.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38720..40603
                     /locus_tag="U2M88_000951"
     CDS             38720..40603
                     /locus_tag="U2M88_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEM8373347.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40678..40962)
                     /locus_tag="U2M88_000952"
     CDS             complement(40678..40962)
                     /locus_tag="U2M88_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEM8373348.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40952..41686)
                     /locus_tag="U2M88_000953"
     CDS             complement(40952..41686)
                     /locus_tag="U2M88_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEM8373349.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41815..42438
                     /locus_tag="U2M88_000954"
     CDS             41815..42438
                     /locus_tag="U2M88_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEM8373350.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42630..43751
                     /locus_tag="U2M88_000955"
     CDS             42630..43751
                     /locus_tag="U2M88_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEM8373351.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43758..46886
                     /locus_tag="U2M88_000956"
     CDS             43758..46886
                     /locus_tag="U2M88_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEM8373352.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46905..50099
                     /locus_tag="U2M88_000957"
     CDS             46905..50099
                     /locus_tag="U2M88_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEM8373353.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50245..50952)
                     /locus_tag="U2M88_000958"
     CDS             complement(50245..50952)
                     /locus_tag="U2M88_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEM8373354.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51717..52115
                     /gene="spxA"
                     /locus_tag="U2M88_000959"
     CDS             51717..52115
                     /gene="spxA"
                     /locus_tag="U2M88_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEM8373355.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        17117 a         9071 c        10793 g        15179 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatcccaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggttt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg agactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatagt caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccatggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttta
    23041 ttttacttga actcattgac tgttttccct ttattttaaa cattataaag accgatgagt
    23101 gcttgccgcg tcttttgttt ttatcacggt tttttgtgca aaaaaacctg ccgatagttg
    23161 gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc tggcaattgg ttttctaaat
    23221 cagttcggac aacaagcaca tcgcaagctg cgtttctaat aacatattca gaaacagaac
    23281 caataaataa acgttctaca gcattcaatc ctgtggctcc taacataatc aaatctactt
    23341 gattatcttc tgggatttct ctggcaataa tttgttttgg tgagccatat tctacgacag
    23401 aggttacatt attcaagcca gctttcttcg cattgctttc ataatctgcc aatgtttgct
    23461 ttgccatttc agtagcttgt tctgcaagca ttccatcaaa ggatgaaacg gtttggaaag
    23521 aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt atttcggata gctacattga
    23581 ccgccttttt aaaagctaat tcagcttcat cagaaccatc tacggcaacc atgatttttt
    23641 tgtattgttg taacataagc attcactcct caagtcaatc gtattgtact cttttattgt
    23701 agaacacttt agggaaaaag agaattattt actgctttgg ttcagcaatt ttttttgcaa
    23761 atcggatact gaaggtggca gaaacaataa ttacgatcag taaaaagacc aaggcaataa
    23821 agcgttgatt gaaaaaagct gttaaaatac ccattacgcc taaaacagca tatgcagctc
    23881 cgtaaaattg taggaatcct tgatttttat catttttgtt gatcaataca aaaaagatat
    23941 cctgtttatt aaataaataa tagctaacga ataataatac taaagtggcg ataatcatta
    24001 aaatcctaat catgaacaaa tcctccaaaa taagcaacaa atagaaaaag caatttgcca
    24061 agagcaaatt gctttattct ttaaaagatg gtaatctccg ttaatgccgt tgtttgtcct
    24121 gtagtattga tcccgcgaat tcaagcaggt gggtcccatg gtcgtagctt cgaactacca
    24181 aatgataagg catgttacca gcagtgacac aagacgggtc ccaagatatc aataaaaatg
    24241 ttcggtcaac acacaaagag gacaattcga acatcacaga tttcccacct ctatagtagc
    24301 atatgaattt aaaaagagca atgagagagc tcgcagaaac agaaccgttt tcttgtctag
    24361 ttttttggag gatgcttttt ccactctttt atacggtaat actgttgacc cagagcctgt
    24421 tcatatttcc ctgtatcttt tggctgataa tactgagcat tcttcagttt atctggtaaa
    24481 tattgctgat tgacccaagc ctgatcaaaa tgatgaggat actgataacc cacgccacgg
    24541 ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat gatcaggaac atctccagct
    24601 ttgccctctc gaatatcagc aagtgctgca tctaatgcca tgtaggcaga attggatttt
    24661 ggcgacaagc aaagatcgac gacgacgtct gctagtggga tacgtgcctc aggcaatccc
    24721 aatttttcag cagctaatac agcgttcact gtacgagccg cagcagcagg attacctaaa
    24781 cctatatctt cataaccgat aaccatcaga cgccggcaaa tacttgccag atcacctgcc
    24841 tcaactaaac gggcgagata atgaagtgca gcatccacat cacttccacg aatcgatttt
    24901 tgaaaagccg aaataacgtc ataatgagca tctccatttt tgtcatgggt aagagctttt
    24961 ctttggacac attcttcaat gatcgacagt gtaagatgaa tccttccttc tttatctgaa
    25021 ggagtcgata gtgtagccag ttccagccca ttcaatgcac tccgcaaatc gccgttcgtt
    25081 gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga ttgcttgttg tccaaggccg
    25141 cgttctttat cccttaaagc atgttcgact gccaactgga tgtcttgttc tgttaatggt
    25201 ttgacttcaa agatctgtgt acggctgcgg atcgctggat tgatggtaat ataaggattc
    25261 tcagtagtcg ctccgatcaa gatgattcgg ccactttcta aatgaggaag cagaaaatcc
    25321 tgttttgttt tatctaatcg gtgtacttca tccaataaga ggatcactgt accactcatt
    25381 tttgcttctt ccgctacaac ttgtagatcc ttttttgaat ctgtagcggc gtttagcatg
    25441 cgaaatgcat aattagtaga gccggcgatc gcacttgcga tactcgtttt tccagtacca
    25501 ggaggtccat atagaatcat ggaagagagc atgcgagcat cgaccatgcg gcgtatgatt
    25561 tttccttccc cgactagatg ctgctgacca acgacttcat cgatcgttcg aggtctcatg
    25621 cgataagcca atggctgttg cataggattt tcctccttct tgtttttacg tcccttaatt
    25681 atatcatatt tagcaagatg cctagaaaga acagccttct gttgttttgt tttcatagct
    25741 tatccaacta atattgtgta tgataaagta ggtgagaaaa atgacggata aacaaaagat
    25801 agatgaattt ttttggaata aaacgacgcc agaagtggca cgggatttat tggggatgta
    25861 tttggagcat gacacaccag aaggaagatt agctggatat attgtagacg cggaagcata
    25921 cctaggtcca gaagacgagg ccgcccatag ctatggttta agaagaacac cgcgtgtgcg
    25981 ggcaatgtat gaaaaaccag gtgcgatcta cctttacacg atgcatactc atcggatttt
    26041 gaacatcatt actcagccag aagggattcc tcaaggagta atgatacgtg caatcgaacc
    26101 cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga aaaactggtc cagatatcag
    26161 caacggtccg ggaaaattag tggaagcatt agcaattcct caagaacttt atggtcagtc
    26221 tatagcagat agttcgttac gcttagtttt tgagaaaaaa aagacaccaa aaaagatcaa
    26281 tgctttgcct agaatcggta tcccaaacaa aggtgtatgg acagaaaagc cgttgcgttt
    26341 tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa aaccaaatcg agaaagattg
    26401 gggttggagg aaagaaaatg aaaaagaagg atctattaac atatttagat gaaataattg
    26461 aaaaacaagt gacagactat gatgtagctt tagattggga tacaaaaaat catacgatcg
    26521 agatcgttgt tcgcttgttt gcagaaaata aagcacaatt tgagattgat gatgcagaag
    26581 ggatcgtttc gcaagaacca attatcgaat ttgaagacgg cgtacttctt tttaatcctc
    26641 agaaatctgt ttttgatgaa cgcaattatc tagcagtaat cccttatgaa ggaaaaaaag
    26701 ggttggcaaa atcagtagct gattctttag tggaatatct aaatgaagta ttagctcaag
    26761 ggcaaagcga tttgcttgat tttttggatg aagacgatga cgaagctgta ttcgagcttc
    26821 actgggataa tcagcaacta gcagaattag tagaagtaaa acagcaaaat gaagcagata
    26881 cttatttacc gtacccaagt tattaaggag tgacagatat gaaatggaat gaagtaaaag
    26941 tagaaactga aagtgaagca gtcgaagcag ttgctaatat tttgatggag gcaggggcga
    27001 gtggtgtcgc gattgaagat tctttagatg ttgaacattt tcagtcggat caatatggtg
    27061 agattttaga taaaaatgat tttacatcgc tgaaagaagg cgcactagtc atggcttatt
    27121 ttccggaaac gactttctta ccggaaatct tgccttttat caaagatcgt atcacacaat
    27181 tacctgagtt tggtctagct attggaaaaa ataaagtaac agttagtgaa gtagaggaaa
    27241 gcaactgggc aacagcctgg aagaaatact atcatccggt gcgtattacg cgaatgctga
    27301 cggttgcgcc aagctgggaa aactaccaga cagaagatcc tctagaaaaa attatcacac
    27361 tagatcctgg tatggcattt ggcacaggca ctcatcccac gacttccttg acattgacag
    27421 cactagaaac agtcttgcgt ggtggtgaaa cattgctaga tgtgggaact gggtcgggcg
    27481 tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt ctatgccttt gatttagacg
    27541 aagtagccgt tcgttcggcg aaagaaaata tggatatgaa tgaaatagcc aaagatgtcc
    27601 atgtatcagc taacgatctt ttgacaggaa tcgagattga aagtgatgta atcgttgcga
    27661 acattttagc agatatcatc ttattgatga tcccagatgc ttggcgttta ctgaagcaaa
    27721 caggcacgct gatcgtttca ggaatcattg aagagaagaa gcaaatggtt ctggatgcca
    27781 tgttagaaca agggtttatc gtggatcaaa ttttccagca aaaagactgg tatgcaatta
    27841 ttttgaaaaa accagaggtg gaatagatgc agcggtattt tttaaaagaa tcatatgaat
    27901 caaaagacta ttttgtttta gaaggggaaa actatcacca cattgtacgg gttatgagga
    27961 tggaacccga ggatgaagtt tttttggcat tcaatgatca aatagccatt gttgcagaaa
    28021 tcacggagat tactgaagag gctgtttatc tgaaagaaat taaaaaagaa gaaaatcaaa
    28081 aggaactgcc aagttcgatt accatagcga gtggctatcc taaaggggaa aagctggatt
    28141 ggatcgttca gaaatccact gaattaggag cgcaagcttt tcttggtttc ccggcatcag
    28201 cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg acaacaacgg cttgaaaaga
    28261 ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt gccatcagta gagttattgg
    28321 aaacgaaaca agatttattt gagcggctag atgattatga cgtggtcttg gttgcttatg
    28381 aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa aattcttagt gaaacaaaac
    28441 cagatagtcg aatcttagct gtctttggac cagaaggtgg attatcccgc gatgaagtcg
    28501 attattttgt agaacacaaa gggatacttt gtggattagg accaagaatc cttcgtacag
    28561 aaacagcacc tctttattta ttaagtgctg ctagctatca atgggaatta atgaatcctt
    28621 ctttcagcta acgttcttca ttaataaaag agtatcgaca gaaagaattg aaaaaatagt
    28681 caacaatttg tataatagaa acaattattt atacaatgga tacgaaaaat cgtttgaaaa
    28741 agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa ctgcacggat tttgggacaa
    28801 ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa aaaagaaagg gggatcgatt
    28861 cctgtgacaa aagaagaaat tttaacggga cccggtgtca taaagctcgt tagtcaatac
    28921 atgggtcccg aacatgtagc atttgttgag aaagcatgcg aatacgcgac tgctgcacat
    28981 gacggacagt ttagaaaatc aggcgaaccc tacattattc atcctatcca agtcgcaggt
    29041 atattagcag atttaaaaat ggatccccat acagtggcta caggcttctt acatgatgtt
    29101 gttgaagata cagaaatcac tttagaagat ctgagagaag aatttggcga tgacgttgct
    29161 atgttagtgg acggcgtaac caaattaggg aaaataaaat ataaatccca cgaagagcag
    29221 ctagcagaaa accaccgaaa gatgctgcta gcaatggctc aagatttacg agttatcatg
    29281 gtcaaattag ctgatagact acataacatg cgtacgttga agcacctgcg agaagataag
    29341 caaagaagga tcgctcagga aactttagaa atctatgcgc ctcttgcaca tcgtctaggg
    29401 atcagccgga tcaaatggga attagaagat acagcacttc gttatctaaa tccaaaacag
    29461 tattaccgta tcgtccactt gatgcagacg aagagagaag aacgtgaaaa atacgtaagt
    29521 ggtactgttg aagatattcg aatagcgaca gaagagctgg ggatttttgc agaaatctat
    29581 ggacggccaa aacacattta ttcaatctat cgtaaaatga aagatcagaa aaagcagttc
    29641 aacgaaattt atgacttgct agctattcga gtgatcgtag attcaatcaa ggattgttat
    29701 gctgtattag gagcaatcca cacaaaatgg aaaccaatgc ccggcagatt taaggattat
    29761 attgccatgc caaaagctaa tatgtaccaa tctttgcata ctaccgtgat cggtccggca
    29821 ggaaatccgg tagaaattca aatccgaaca caggaaatgc atgaaatcgc tgaattcggg
    29881 gttgctgcac actgggccta taaagaagga aaaaatgaaa aagtagaacc agatggtatg
    29941 acgaaacaat taagctggtt ccatgagata ctcgaacttc aagacgaaag ctatgatgct
    30001 tctgaattta tggaaggcgt aaaaggagat atctttagtg ataaagtcta cgtcttcaca
    30061 ccaaaaggag acgttactga gttaccaaaa ggatccggac cattggactt tgcatacagt
    30121 atccatacag atatcggtaa caaaaccact ggtgcaaaag taaatggcaa aatggtgcag
    30181 cttgattaca aattgaaaaa cggagatatc attgagatca tgacttctcc aaattcattt
    30241 ggcccaagtc gcgactggtt gaaattagtt gctactagca aagcaagaaa taagatcaaa
    30301 cgtttcttca aagcccaaga tcgagaagaa aatgtgatca aaggccacga atccgtggtc
    30361 aaatgtatta cagatctagg atttacgcct aaagatattt tgacgaagaa caaactgcaa
    30421 gaagcactcg atcgttttaa ttatcaaaca gaagatgatc tctatgcagc tgtagggtat
    30481 ggagaagtta gccccttgac gatggccaat cgtctgactg aaaaagaacg taaagaacaa
    30541 aaaatcgagc agcaaaagca agaagcagaa gaaatcatga atcagccgaa aaaagaacct
    30601 gacaaaatga aagtacgtca tgaaggtggc gttgtcattc aaggggtaga aaacttacta
    30661 attcgtatca gtcgctgctg taatccgatt cctggtgatg atatcgttgg ttatatcact
    30721 aaaggcagag ggatatccat tcatcgtcga gattgtccga atgttcagcc tgacaaacca
    30781 aatgtagcag aacgtttgat tgaagtcgaa tgggaagata catcgaatac acgaaaagag
    30841 tatgatgcag atttggaaat ttacggctat aatcgttcag gcttattgaa tgatgtactt
    30901 caaacagtca atgcgctaac gaaaaatctc aacagcgttg aagcacggac gaataaagat
    30961 aaaatggcga cgatccattt gacggttggt atccagaatt tatcccacct aaagagtatc
    31021 gtggataaaa tcaaagcagt acctgatgtc tacagtgtac gccggacgaa tggataggag
    31081 gacagatatg agagcagttg ttcaacgggt aagtaaagct gctgtgtcag tcgatcagca
    31141 agaaattgga aaaattggct acggcctact tgttttatta ggcattcatg aaaccgatac
    31201 gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa atgcgtattt ttgaagatga
    31261 acaaggaaaa atgaatatta gtgtagaaga tgtgggagga caaattttaa gtatctctca
    31321 attcacactg tttgcggata cgaaaaaagg aaatcgacca agctttgtgg aagccgcacg
    31381 accagaaaca gctattcctt tatacgaatc attcaatgaa ggaatcagaa gtcgcggcat
    31441 cacagtcgct accggacaat tcggagcaga tatgtcggtt tctctcgtca atgatggacc
    31501 tgtaacgatc attattgatt ctaaaaacaa ataaagtaat ctggttttca ctagtaaaaa
    31561 ctatgaaaca cgtcgcttaa gttagactga taaactctaa cttaagtggc ttttttgtct
    31621 gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt aagcttaaat tatacaagtt
    31681 attttttata ggaggttgct gaagatgaaa gaagtaccag tagtagatat caaaatcaca
    31741 gatttaaaaa aattgtatca aaaaggatac aacatgttgg aggagttacg ccatgaagcg
    31801 gacgctcccg tcgtcaaagc aaagattttc aataaagaag ctatcacgat ctacggatca
    31861 tcagcagcaa aagtatttta tgatccaagg aatttcaaac gaaaaggtgc tatgccaaaa
    31921 ttagtattga agacactttt tggtcaaggt ggagtacaga ctttggatgg agctgctcat
    31981 caccatcgga aaaatatttt tatggatcta atgacaccgg aaagaatgga agattatcat
    32041 cgtatcttag ataagaatct gacacaagca cttgaagcac agcatggtca atttgaacta
    32101 tttgatttaa gtaagatggt tttctttaca tctatttgcg agtgggcagg tatcaattta
    32161 tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat atcaaatctc tatgatcagc
    32221 ggaacgttta cttctccgat tgatcacata aaaggtgtcg aaaatcgcaa aaaatcagaa
    32281 aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc ctgtggctgg aaaagaaaat
    32341 gtggcattat atgcttttgc caatgccaca gatttagatg gtcagctttt acctctggaa
    32401 gtggcagctg ttgaattatt gaacatcatc cgtccaacgg ttgctctaac tgtgtgggcg
    32461 gcattgatgg gacatgcact tttcagtaga ccagatttgt atcagcaatt aaaaaatgac
    32521 ttttcgacgt tacaagatcc gtttattcaa gaaatgagac gctattatcc tttcttccca
    32581 atgctacctg cgatttcgct gaaagaagta gaagtagatg gttatcgtat accagaaggc
    32641 agttgggtta ttttagattt atatggaaca gaccatgatg aacgaacagt tgaagcgcca
    32701 gattccttta tgatcaaacg gtatgtaggg aaagcaaaag atatttccta taaagaagaa
    32761 tatgagatga ttgcacaagg cgggggaaat tttcgacaaa tgcatcgctg tgcaggagaa
    32821 tggatcactc ttcatagtct acgtgtgttt tccgatcagc tagtaaataa gtttgagttc
    32881 tctgtacctg aacaagattg gacgattcca ttcaatcagt ttccgactta tccgaatagc
    32941 cgagcgctac tttacaagaa ttagattagc ataaacttat cgaaaaaaag gaagatcaaa
    33001 cagtgctgat cttccttttt tcatattact ataaattgat aaggtcaatt gatcaaaatc
    33061 cacgaaatgc ttcatcaatg gtttgataat cttcattgga taaatgcaaa tctaaagctt
    33121 ggacattgtc agatacttgc tctggacgct tagctccagg tataactaca gacactcgtg
    33181 gattcttcat gtaccaagct aaaacaagtt gcgcaggagt ggcttggtat cttttagcaa
    33241 tggggcgaag tacatcgaca gcggttaaaa tttctctgaa acgttctcct tggaaatctg
    33301 gatttctttt tcgaggatct ccttcaccaa attgtttttc ctcaccaagt tcatattttc
    33361 ctgtcagcaa accggaagca agaggaaaat agggaacaaa actgattttg tttttttcaa
    33421 gataagggaa taattctttt tcagcctgcc gatggatcaa actgtattta tcttctacta
    33481 catcgacata tccatctgca ttcgcttctt tcaattgttc taacgtgaag tttgacacac
    33541 cgactgctcg gatttttcct gcttctttta attcgtgcaa tgtagcaact gattcatttt
    33601 ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc gatgtaatca gtttgcagac
    33661 gttttagtgc atcttccacc gattgtttga gaaactcagg agaattatca aatgttcgcc
    33721 ctttatttgg tacatgagca gcttttgtag cgatgacgac acgtgagcga tcatattctt
    33781 tttccttcag tacctcgcca atcagttctt cggaacggcc gtttccgtaa gcataagcgg
    33841 tatcgatcaa attgatcccg ctattcaaag ctgtacgaac tacttgtttt cctgtctcat
    33901 cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat ccaagctgtt
    33961 ctgcgtagac ttgtgaatgt ccgattctta tttctgccat agctgattcc tcctttttgt
    34021 ctagtctaaa acaaaatacc aaaaagctga actaaaacgt ttttgtgagg ggaaatagtc
    34081 agatatctct tgactttgag actggttttt agtaggttat aagtaatatc gaatgtcaat
    34141 gaaagagcga gtaagttttt gaaactttac aagagaggat catcgaggct gaaaatgatc
    34201 taaagtttca ttagcctaaa gacactctgg agatggacga gtgaaaagcc gtcccgcgta
    34261 cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat taggtggtac cgtgtatgaa
    34321 tttcattcac ccttgttcat gatgaacaag ggtgattttt ttatttacat agctaagaaa
    34381 gggagagaaa actatgagtt tccaaagacc aaaaggaaca aatgatattt tgccaggcga
    34441 atcagaaaaa tggcagtttg tcgaagaaac agcacgttta ttattcagag attatcagta
    34501 taatgaaatg cgtacaccga tttttgaaca ctatgaagtg atatcccgaa gtgtagggga
    34561 tacaacggat atcgtttcaa aagagatgta tgatttttat gacaaagggg aacgccatgt
    34621 gacgcttcga ccagaaggta cagcaccaat cgttcgttct tttgttgagc ataaattgtt
    34681 cggcccagag tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt ttcgctacga
    34741 acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag catttggatc
    34801 agaaaaccct gcaactgatg tggagagcat ggtgatggca cttgatttct tcaaacagtt
    34861 ggggatcacg caaatccggc tagtcatcaa ttcattaggt gataaagaaa cgcgtcaagc
    34921 ctatcgtcaa gcattgatcg actatctttt accttttgaa tcagagctga gtgaagactc
    34981 taaacgccgc cttcatgaaa atccattacg cgtattggac agtaaagaca aacgagatca
    35041 aaaatttgtg gcagaagctc catctatctt agattatcta agcgagcctg ctcaaaaaca
    35101 ttttgatacc gttgtttcca tgctggatgc attggctgtt ccttatgaaa tcgacagtaa
    35161 tatggttcgc ggactagatt attacacaca tacgattttt gaaatcatga gtgacgcacc
    35221 aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc tagtggaaga
    35281 actaggtggt ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc gattactctt
    35341 gactatggag gcagaggaag tagttatccc tgcatttaat gagttggatg cttacgtggt
    35401 cgcactcgga gacgaaacaa atatcgaagc attgaaagtg gtccaagcga ttcgaaactt
    35461 tggtttttca gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat ttaagacagc
    35521 agataaagcg aatgccaaat tagtattggt gatcggtgaa gatgagcttg ctagtcaaac
    35581 gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt tttccattag aagagatata
    35641 tgaacatttt gatgaagtct atgatgaaat gactttattc gagtaggagg aaaaaaataa
    35701 atggcacaaa gaacgatcta ttgcggatta gtttccgaac aacaattaga acaaacagtt
    35761 acattaaaag gatgggtaca aaaaagacga gaccttgggg gagtaatctt tatcgaccta
    35821 cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa agaagcatgg
    35881 gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag tgactgggga agttagaaga
    35941 cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg aattcgaagt gattgcaagt
    36001 gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc aaattgaaga tgatcaagca
    36061 atcagtgacg aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc aaaaatgact
    36121 aaaaacatca aattaagaaa tgcgacaaca aaagcgattc gtcattattt agatgataat
    36181 gactttctcg atatcgaaac accttaccta gggaaatcta caccagaagg cgcaagagac
    36241 tacctagttc cttcacgtgt ccatgctgga catttctatg cgttgccgca atctcctcaa
    36301 ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt attatcaaat cgttcgttgt
    36361 ttccgcgatg aagatttacg tggagaccgt cagcctgaat ttacgcaagt agatattgaa
    36421 acaacgttct taacagcaga agaaatccaa acttacacag agggattgat tgcgaaggta
    36481 atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac ctatgatgaa
    36541 gcaatggctc gctacggcag cgacaaaccg gatacacgtt ttgatatgga attgatcgat
    36601 ctaagcgata cagtcaaaga agtagaattc aaagtcttcc aaatggctct tgaaaacggc
    36661 ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc gctactcacg aaaagacatg
    36721 gatcagttag gtcaatatgt aggacaattc ggagctaagg ggcttgcatg gctgaaagta
    36781 gaagaagacg gattgaaagg tccaattgct aaattcatgg gagaagcaac agaagcaatc
    36841 attaaagcaa cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga taaaagtgaa
    36901 atcgtcgctg ctgcattagg tgctatccgg acacgtttag gaaaagaatt aggactaatt
    36961 gatgaatcga aattcaattt cttatgggta actgattggc cacaatttga attttcagaa
    37021 gaagaaggtc gatatgtctc tgctcaccat ccattcacga tgccaaaaga agaagatatc
    37081 ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat cgtcttgaac
    37141 ggttatgaac taggcggagg ttctatccgt attcatactc gtgagcttca agaaaaagta
    37201 ctagaaactc taggctttac aaaagaacaa atggaagaac aatttggttt cttgttaaca
    37261 gctttagatt acggattccc tccacatggc ggtattgcat taggattgga ccgttttgca
    37321 atgttattag ccggagaaga taatattcgt gaagtcattg cctttcctaa aaacggaaaa
    37381 gcagcagatg tgatgagtga tgcaccaagt atggtttcgc ctttacaatt gttcgaattg
    37441 aatattgatg taacgaatat agaagaatag ccgataaaaa ggtaaaaagg atttgtcgaa
    37501 tacgcttctt ttgacaacct tgatttaatt gaaaaacact ttataatgaa gccataacat
    37561 ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca tgattacacg
    37621 accaagcttt cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt gaatttcttt
    37681 tatcagccag gacatattta ttcaagtggt attactggtc tagctcagat tttgacgact
    37741 ttgtcaaaat cactgatagg attcgaagta ccggtatcaa caactttgta tttactgaat
    37801 atacctttgt ttgtattggc gtggttaaaa atcgggaaga agtttacaat tttcactttt
    37861 atcacagtca ctttgacctc cctttgtatc catattttac cggaaacagt cctatctacc
    37921 gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat tgggctagcg
    37981 ttgagaaacg gcctatcatc gggcggtcta gatatcgtga gtatcacgat tcgtaaaaaa
    38041 acaggaaagt cgattggttc tatttcgatc tacttcaatg cattgatcgt ttttgttgcc
    38101 ggaattctat ttggctggca atatatgttt tacagtacgt tgtctatttt cgtcagtgga
    38161 aaagtaactg atgtcgttta tactaaacaa aagaagatgc aggtaatgat cgtaacgaaa
    38221 catccagaaa aagtagtaaa tgaaatccaa aactgtatgc gtagagggat cacgatcatc
    38281 aatgaagcag aaggtgctta taagcatgat aagcagacag tattgatgac gatcgttaca
    38341 cgatacgaac tgcctctact tagagaagca atgaaagctt ctgatccaca agcttttgta
    38401 agtattgcag aaaacgtcca gatacttggt cgtttctatg aggatggcat ttagatcgag
    38461 tttgacaaaa attcatgaaa tactgctgta gacagacgtt cgtttgtctg cagttttttt
    38521 ctaccatagt agaatcagca aagaaaaact gcttttttat ttgaaatttt cttaaagttg
    38581 aaaaaaagca tgtttatttt tttaaattta tgggatgata gaaaaaaata gtagagaaag
    38641 gacacgattc tacttgctat tttcacttaa tacccgtata ttacgaatga aaaaagttta
    38701 tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc gctacatcac
    38761 tgggtttgat gggctccgtt cattagctgt ggtgggtgtt atcttctatc accttctgcc
    38821 aactagtatg aaaggcggct atttgggcgt tccgattttt ttcgtcgtat ctggttattt
    38881 gatcacggat ctgctgcggc aggaatggga acaaaatgga aaaatcaata tttggcaatt
    38941 ttatgtcaga agaatgaaga gactctatcc ggcacttgtt tttatgcttg tgacagcttc
    39001 ggcttatatc actttgtttc agcgagggct gttaaacaat cttcgaggag ttgtggtcag
    39061 cagcttactg tatgtcaaca actggtggca aatcaataac ggtttatctt atttcgatcg
    39121 atttgctaat gagtcaccgt ttacgcatat ctggtcttta gcagtagaag gtcaaaacta
    39181 tttggtttgg ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa gaaaatggat
    39241 cttttatact gtgttaggta catccttcct ctccgccatc ttgatgatga tcttgtatac
    39301 accaggaggc gatccaaccc gtgtttatta cggtaccgac actcgtctgt tttctatatg
    39361 gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa acatcccgaa
    39421 acaagcgaaa aatattctga atatcgctgg aggaatctcg cttttggcac tgattctttg
    39481 cttcttcttt ttagatgatc attacaattt catctattac ggaggaatgc tcctagtgag
    39541 tctgttatgc gtagtgctag ttgcagtgac agcacacccc ggagcaagtt tgaatcgatg
    39601 gctgacaaat ccagtattta cttggattgg caaacgaagc tatggcattt atctgtatca
    39661 gtttccagtt atgatttttt atgaagcaaa ggtgactgat attgccgatc atgttttgtt
    39721 gcatacttta gtggaaatca cattgattct aatcatcagt gaactttctt atcgatatgt
    39781 ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc caagtgatga aaggatggtt
    39841 cagcaaaccg atcatcagta agagcaaacc atgggttgtt ccaggcacat tacttgtagc
    39901 aattgcactc gttggtttcg ttacggcacc gaaaaacgct gtaacagccg atcaaaaaca
    39961 actgcaggca aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa acaattcaga
    40021 aaacaccact agttcggttg accaagcgat ccttgataag tacgagttgt ccaaggcaca
    40081 agggaataag gcacaaaaat tagaactaac ggcatttggt gattcagtta tgttggatgc
    40141 tgcaacggat ctacaagaac tttatccaaa agtagtggta gatggagatg tcggacgaca
    40201 attatatgcc agtgtaccgt acattgaaaa attaaaggaa caagatttgt tgaaagacac
    40261 tgttctgatc ggactaggaa cgaatggctc attctcggaa gctcagtttg atagtgtgat
    40321 ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga cgaagcgctg
    40381 gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca atatgacgct
    40441 gattgattgg tacagctaca gtaatgacca tagtgactgg ttttatgatg atcaagtcca
    40501 tccaaatcct gaagggatga cacattacat tcacttagtc agcgaagcct tgctaggagg
    40561 aagttcaagt acgaacagca gtgagacaaa cgtgacagaa tgatagctac aaaaaacttc
    40621 aggcaaaaac atttgttttt gcctgaagtt tttttatatt taccgtgttt ttaacattca
    40681 tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt tttgttttcg
    40741 ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag aaaatacttc
    40801 tgcatggatc atcttaacag gtcttctttt ttgaagacga gtatatttag caccagtacc
    40861 attgttatgt tcctctaatc gtctagaaag atcggttgta tatccaccat aaaatgtctg
    40921 atctttacag aataatacgt aaaaataatg ttcatttgcc atagagcatc tcacgaatct
    40981 ctgcagtgta ttctccatcc atattgtaag tgatcaatgg tggagcgact ttgaatccgt
    41041 ctaatttgcc ttctttgatt ccttcaacta acaaaatatt tgcctctttt ccaggttttg
    41101 ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc tgaatgattt
    41161 ctaagaaccg atcaggacga tggaccatag taaagcgtcc atttgttttt agtagcttac
    41221 ttgaaatatc gatcacttct tctaacgtcg tatgaatctc atgacgggcg attgccaggt
    41281 gtttattagg gttcgtttta tttgttggca gccctttgaa ataaggtgga ttacagacga
    41341 ctaagtcaca agaattatgt cgaacgacag ataagctgtc tttcagatcg atggtgtgga
    41401 cagtcatttg ttcatttaaa tggttaagcg cgatactacg tcttgccatg tctgctagac
    41461 gaggttgaag ttcgatagcg tcgatagccg cctgggttcg tttacttaaa aaaagtccaa
    41521 ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca cgggaaaagt
    41581 gagcaagcag cacagcatct aaagaaaaag aaaacacttt ggaactttgg ataatcttga
    41641 tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg aagcatggat tccctccaaa
    41701 atgaaaaaaa tagtcgtctg tattatacta tttttattaa aaagaaccaa ggtggcttgt
    41761 caaacctcta ttttttatgc atactatgac cataagaagg gaaaggaaga gactatgttt
    41821 tatcgattta tgcgcggggt agttcgagta ctattagcct tagttaatgg aaatgcccgc
    41881 tatgagaata aagaagtttt accgcaagat gagaattaca tactagttgc tccccatcgt
    41941 acttggtggg acccattata tctggcggta gctgcagcac ccaaggaatt cagctttatg
    42001 gcaaaagaag aactgttcaa aaatccagtt ctgcgattta tattaactcg atccaatgct
    42061 tttccagtta aaagagaaaa tccaggacca agcgtcatta aaacacctgt aaagattttg
    42121 aaatcgacaa atttgagttt gatcatgttt cccagtggga caagacatgc tacagaactt
    42181 aaaggcggaa tggcattgat ttccagaatg tctaaagcga aaatcgtgcc agctgtatat
    42241 caaggtccgt tgacacttgg ggatctgttc aagcgtcaga gagtgacagt gcgtttcggc
    42301 gaaccaatcg acttatcaga tatcaagaaa acggataaag aaggtatggc ggagatcgaa
    42361 cgacgaacac aagcagcttt tgacaaattg gacaaagaag tgaaccctga tttcaaatat
    42421 gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc ggtcttttga atctactgtc
    42481 ctgttcagtt cagacttttt caatgaaaac gagggagaga cgtcaagtct gccctctttt
    42541 tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag tgttataata
    42601 attagaagga cattttttga ggtgattgaa tgcgtttttt acatacagcc gattggcata
    42661 tcggaaaaaa actacaaggt tatgacttga ttgaagacca gcgtcatgta ctatcttcta
    42721 tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga gacttatatg
    42781 ataggagtgt cccagctgtt gatgccgtag aattatacaa tgaattgatg gtcgattgga
    42841 atttaaagga aaagatcccg atttttgcta tatcaggaaa tcacgatagc agtacgagac
    42901 tttcagcggg tacgccatgg ttcagttatt cgaactattt tttgcgaaca cagctagcag
    42961 aagcatttga accagtcgag tttaaaaacg tacaattttt ctttcttcct tactttgaac
    43021 caattgcagc gagattatat tttgaagatg acaatatccg aacgatcaag caagcggtag
    43081 ccaaggtgat agataaaatg caggagctat ttgattcaga aaaaaaacaa gtattgatta
    43141 gccatttttt tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg
    43201 ggttggattc gatccctggt gaactactta caccgtttga ttatgtagca ctaggacatt
    43261 tgcataatca agctgctttg agacaggata atgctcgcta tagcggttcc ccattgaaat
    43321 tctctttatc tgagatcaac caaaaaaaag gcgtatggat cgttgacctg acaacaggcc
    43381 tggagttgaa ttttagggag atcgaacccc tgcatgatat tgaagaaata acagcgagtt
    43441 tcaaagaatt gctggatcct gccttttatc agtccgtcaa tcgggagaat tacatctata
    43501 tctatctcac tgatagaacg atcatcccga atatgatgaa ccagcttcgg caaatatatc
    43561 ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa
    43621 aagtactaga aatgaaagat cctcaaaaat tagcagaagc attttttagt gaaatgacgg
    43681 aagagcagct aactgcaaac caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg
    43741 aaatggaatg atcaagaatg aaaccgaaaa aattacaact taaaaatttt ggtcctttta
    43801 tcaatgagac cgtagatttt tcacgtttga cagaagcacc gctttttttg atcagcggga
    43861 agacaggagc tggcaaaacg acgatttttg atgggatcac cttcgcactt tttggtgaaa
    43921 cttccggaag acttcgctct ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag
    43981 aaacaagcgt gactttttct tttgaacatc agcagatgag ctatgaaatc gaacgaaaac
    44041 cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa
    44101 cgttgaccat ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa cgtacagaag
    44161 cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa acagttttct caaatcgttt
    44221 tactacccca aggagaattc cgtaattttt tggtttcatc aagcagtgat aaagagactg
    44281 ttttacgtca tttattcggt actcaatttt tccaacagtt caatgagcga ttaaaagaaa
    44341 aagcaaaaca acagcaacaa aaattggatc atttagagca agagctgcat cttctgcaaa
    44401 aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc gttttagccc
    44461 aatggacaaa ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa gaagctctaa
    44521 cggaacttca ggaaaaacaa aagcaactag aaaattccta ctatcgtttt gaaagcttga
    44581 aaaaaagtta tgaagaaaaa acgatattaa taaacaagca aaaggaatta gctgctcaaa
    44641 aagacgaaat agaggaaaag aaacgatgga ttgggtatta tgaatttacg caacgattag
    44701 tcgaaccaat ccagcgccta caagaaataa aagttgaaaa aatagaactg tcacaaaaaa
    44761 gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag tggcaaaaaa
    44821 atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa aaactgcaaa
    44881 acaaacagat gatggtgcca attgtcgaat ccttacgtag taaaaaacaa gacgcagaac
    44941 aattaacaat ttcattggat aaagaagaat tgaatgccga gaagctgtat caaaatctcg
    45001 ctatctatca ggaaagaact cgaattcttc aagaaaattt actggatcag gaaaaactgc
    45061 aagaagatcg tcttgctttt ggacagttaa aatatctaga tgaaaagcag caagaaatag
    45121 aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat aagagaagaa ctcaaaaaag
    45181 caactgaaga aataacaaaa aatagtcagc tattgaaaga acagacagaa caagcagaac
    45241 agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt ttgcgtccag
    45301 gggagccatg tcttgtttgt ggatcaacgg aacaccccat acaacaatta aaacacgaac
    45361 agccaaataa ggaagatatc ttaaaagtag aacaacaact ggcagaagct gagtctgctg
    45421 tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat cttcaacaac
    45481 aattatcaga tttagaacaa aaaatcatgg caacgaatat acaattggct gaacaagaag
    45541 aacacttgca acaggctttg aaaaaggcat ttcctgagat aacagaacaa aaaacaaaaa
    45601 caactcttgt agtaatcgaa caatctttag atagacgaaa aagcaagatg gccaatgcaa
    45661 gaatagagct ggaagaaata agacaacaag cagaaaagct cgaacaacaa ttagctgtct
    45721 acaaacagca gcagacagaa aaaaataatc agctccagca actgcaagga gaaatcaaaa
    45781 gtttagaaca acaattgcaa gatacggata gtaactgttt agaagaggag atacgacgac
    45841 ttgaaaaaca gttagaagaa gactcgaatc aactagctgt tgagcagaaa gcaggagtgc
    45901 aattgttgca agatcttcat acgcttcgga cacagcttga tcttcaacat gaacaaatag
    45961 aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag ttagaaagcc
    46021 agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa gcacaatata
    46081 aagaaaacca acgtacagtt acttcttatc atgaagagca attagtgaca gctgaccggc
    46141 tgcgtcaatt agaaaatatc cagctaactg agacattccc agatacagaa gagatcagcc
    46201 aacagttaga agaaataaga cgtatgatcg aaataaagca agaaaagata tacggtttac
    46261 aagaacaaaa aattagtaat caaaaaatct acgatgaatg tctttcgatt tatcacacaa
    46321 gccagacgaa attagatgag cttacgcaat tacagcagct ttcgcaaaca ttcaacggag
    46381 aaaatcctaa gaagactagt ttggaacggt atgtgcttca ggtttatctt caagaagtgt
    46441 tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa tttgagctag
    46501 cggataccat tggcagttat cgagggaaaa ccggattaga aatcaatatc tatgatgatc
    46561 aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt attgcagcac
    46621 tttctttagc attagctctt gccgatgtga tccagacaca agctggtggt gtagcaatcg
    46681 atgcattgtt tattgatgaa ggatttgggt cattagatga agaagctttg gaaatggcaa
    46741 tggaagcttt agaaacgatc gaaagtgaag gaagaatgat cggcatcatt agtcatgtaa
    46801 gagaattgaa agaacgagta ttacaacaaa taagaattga tacagaaggt agcggacaaa
    46861 gcaaaatacg ctatcaactt ggataaaaag ggagattggt aactatgaaa tggagtattc
    46921 cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga aggccgagtg ttatcggttg
    46981 tccctgacca agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa ttgtatatgg
    47041 ttgatttgga tggtacagca aaagagatag attactgcca atgtccctat tgggaggaac
    47101 ataaatattg taagcataca gttgctgtgg aactttattt acggtcgaaa aatctatctc
    47161 gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa agcaacaagt gagggagaaa
    47221 tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt
    47281 tgattattga ataccaagta gaaacgattg agactaatca gtatcaccca gagctttcta
    47341 ttttggcact ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa
    47401 aaaacatcta tgatttttta caagcttaca cagaaaaaga aacttacact gttaataaac
    47461 agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc
    47521 atcttgcagg aagtgctcag acgcatcaat tattaggtac gaacggctta caagtcaaag
    47581 gaaaactaga taaaagatat ctgcttctcc cagtagaaca agcccgcttc ctgttagagc
    47641 agatgaatca gactacacgt ttgcatctgc agataaatga acaaaacgat caaggaatac
    47701 aattttccga tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga gaagattatt
    47761 tgctgcaagc attgaatgat tttgattttt tctttatgca ttatcagtgg ggagttatcc
    47821 aagggacatt ttattctctg acgaaatatc aacaaacggt ttatcaaact tggaaacaat
    47881 tgattcgccg attggagaaa ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt
    47941 ttaaagagat tatccctctg ctgtcagaga tcggcgacgt agctattgcc cctgcagtag
    48001 aagaggaagt agcagatatc cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa
    48061 aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat ctattcgact gacccgaagc
    48121 atgaggtctc gacagaacag gcttttcagg tgttacgtga cacagtagaa gagaaacgag
    48181 tacttgatct attcagaatg tatcgctacc agaaaaatga aactggttat gaacgtccat
    48241 tgccaagcgg ggaagaacta tatgcctttt tccgcacaga actatctgtc ttccgtcagt
    48301 ttggtgaagt cagacttgga agaaaattgc gagagttgta tttagatgcg cataaacatc
    48361 aaccgaaaat cgaagtaacc gagaccggtt catggctcga tattcgattc gatgtgacag
    48421 ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt
    48481 atacattaga aaatggtcag gttctttctt ttgattcaga agaatttcaa caaacaagcc
    48541 aagtgttgca acaattacgg gaatcgatac gtacagaaga aggaacgatc catgtaccta
    48601 aaaatcaagg attgatcatt caaaatcaat tggaaaaaag caatgctact tttagtgaat
    48661 cgttccaaac gatggtacaa gatctcattc atccagaaag ataccaggca cagcttccca
    48721 aaggattgaa tgcaacgatg agagattatc aaaagcaagg ttttcgatgg ttgaagatgt
    48781 taggtcatta tcagtttggc ggtattttag ccgatgaaat gggacttggg aaaacactac
    48841 agacaattgc ctttttactt tcggaaaaag aagaacggaa aagcttctct gctttgatcg
    48901 tagcgcctgc gagcttaatt tacaactggc aggcagaggt gagaaaattc gccccttctt
    48961 taagtatcca agtaatcaat gggaataaaa aagaacgtga agaattactg gctaaagaca
    49021 cagatatccg agtgacttct tatgccagct taagacagga tctagctaat tatcagtcgc
    49081 aaaaaattga ttatttgatt cttgatgagg cacaaatggt caaaaatagc agtacgaaaa
    49141 ctgcacaagc attgagagaa ttagctgtac ctcagcggtt tgcactgagt gggacaccaa
    49201 tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac gattatgcca ggatttttcc
    49261 cgactaaaac aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt
    49321 ttattttacg gcgagataaa caaactgtgt tgaaagactt gccggaaaaa acagagatga
    49381 atctatattc tgcacttaca gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa
    49441 tgcgtgaaga aatcacatca atggattctg aagcattcaa gaaaaaccgg atcgggatac
    49501 ttgctggtct gacaagactg cgacaaattt gctgcgatcc tcgtctgttt attgaagatt
    49561 accaaggcgg ttccggcaag ctcgaacaag taaaagatct actggtagct gccaaagaga
    49621 acaaacgaag agttttgctg ttttctcaat ttacagggat gctgactatc ttgcaagaag
    49681 agttagccga gctagggatc tcgacatttt atttaagagg cagtacgaaa ccacaagatc
    49741 ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg atttccttaa
    49801 aggctggggg aactggattg aatctcacgg gagcagatac ggtcatcttg tatgaccttt
    49861 ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa
    49921 atgtggtaga agtgtggcgt atgatctctg aaggaacgat tgaggaacgc atggattcac
    49981 tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa gaacagttga
    50041 cgaaactgac tgaagaagac atccgcatga ttttaagtgt aggagaaatc gaggagtaag
    50101 agactcgaaa aagcgttcag taatgggtaa gaaagccttt ctaaagaagc gattgtttat
    50161 ttgatttaaa aaatagcaag gaggctggga ttccatttta tcccagcttc cttgctatca
    50221 tttttttatt tctattttta aaggttatac aaagattgtc ttcattgtct ctaacagctg
    50281 atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt tcaattttgc gttctcgatg
    50341 gttgaaccaa agcgcttgcc agttagcact ttttgcacct agaacatcat tgtcgtaatt
    50401 atcgccaacg tataaggtct cttctggaag catatgaaat tctttctcag ctagctgaaa
    50461 aatctctttt tctggttttt ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg
    50521 aatccaattg ttcaattgaa gttgcttcag tttttttgtc tgatggtcag tcggcccatt
    50581 tgtgataatt cctaatggga cattcttttc cttcaagaaa tcgagggttt ttttgacctc
    50641 ctcatgcaag cagatattat caagttcttc ctcgtaaatt ttttgaaata agaggccatc
    50701 ttcttccgta atatgagggt agtctaaatc ttttagtgat tggctgatcc gatgtgctcg
    50761 catatattct agtgtccaat ctcctgccat tacttttggg aagttttcat cactatgatg
    50821 gcggaaccgg atatatagtg gatgcatatc tgcatctgaa acaagcggaa cgactctttt
    50881 gaccgcgttt cgaaatggct gctgctgatc atacatcgta tcatcaacat caaacacaac
    50941 tgatttaatc aaaattttac acgcctttcg gttttcattg attttttcac aaatccttga
    51001 ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt cttactatat
    51061 ttctatttcc ttgacagaat aaggttttta tatttcagtt tttttcacta aaaatcgtga
    51121 aacacgtcaa taagtttgat gaacaaatta atcattatgg tagaagtcga ttgatttatt
    51181 ttaatgcaat ctctgtggaa ttttcgaaag atttattttt ttaatacagt ctcaatataa
    51241 ctattcaaac ataaaataac gttatttgat ttatttgttt atagtaaatt ttatttagta
    51301 atcattgctt taaaatacta cgcttatata acagagaaaa taagcgttat aataaagaac
    51361 agtattattt aaaatgacag aaaacattac tattaaagaa ataaaaaaca taaaaaatct
    51421 taagaaatcg gtttcattta gcttttgaga taaaataata gtacagtttt atacgctgtt
    51481 tcatatgttt agaaaatttg aattagattt gaaaactcta atttttaaat gataaaaaaa
    51541 gcgagaatgc tggttttact gtatattaac cttagataac tttcattttt ttaaaaaaat
    51601 tatgaaaaaa aggtagctat ttaaaagttg attatgtata atagaaacag atgggagatt
    51661 attatgatct tctgttacaa aaaagtgatt cttatcacga aaaggagtgg tgcacgttgt
    51721 taacattata tacttcccca agttgtactt cctgcagaaa ggctcgtgcg tggttgcaag
    51781 aacatcagat tccatttgtt gaacgtaata ttttttcaga accattgaac agttctgaat
    51841 taaaagcgat tttacaaatg acagaagacg gaacagaaga aatcatctcg acacgttcca
    51901 aagtattcca aaaactgaac atggatttag atgatctgcc gttacaagaa ttattggaac
    51961 ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat gattgatgat aaacgtctgc
    52021 aagttggttt taatgaagat gaaattcgtc ggttcctacc gcgcgatgta cgacaattag
    52081 aattacgtca agcacaattg atggctggat tatagaagga taatacgaaa cttggaaaag
    52141 actattacaa agatagtttt
//
LOCUS       DASHRQ010000015        47854 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.015, whole genome shotgun sequence.
ACCESSION   DASHRQ010000015 DASHRQ010000000
VERSION     DASHRQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.015"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(38..1189)
                     /locus_tag="U2M88_000960"
     CDS             complement(38..1189)
                     /locus_tag="U2M88_000960"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEM8373356.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="U2M88_000961"
     CDS             complement(1202..1639)
                     /locus_tag="U2M88_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM8373357.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="U2M88_000962"
     CDS             complement(1654..3420)
                     /locus_tag="U2M88_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEM8373358.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="U2M88_000963"
     CDS             3684..4361
                     /locus_tag="U2M88_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8373359.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="U2M88_000964"
     CDS             4364..5491
                     /locus_tag="U2M88_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8373360.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="U2M88_000965"
     CDS             complement(5691..6131)
                     /locus_tag="U2M88_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM8373361.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="U2M88_000966"
     CDS             complement(6142..8175)
                     /locus_tag="U2M88_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEM8373362.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="U2M88_000967"
     CDS             complement(8177..9880)
                     /locus_tag="U2M88_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM8373363.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="U2M88_000968"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="U2M88_000968"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEM8373364.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="U2M88_000969"
     CDS             complement(10963..11988)
                     /locus_tag="U2M88_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEM8373365.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="U2M88_000970"
     CDS             complement(12197..13066)
                     /locus_tag="U2M88_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEM8373366.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="U2M88_000971"
     CDS             complement(13525..15417)
                     /locus_tag="U2M88_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8373367.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="U2M88_000972"
     CDS             complement(15414..17141)
                     /locus_tag="U2M88_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8373368.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="U2M88_000973"
     CDS             complement(17157..17807)
                     /locus_tag="U2M88_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEM8373369.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="U2M88_000974"
                     /pseudo
     CDS             18011..18307
                     /locus_tag="U2M88_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            18479..19099
                     /locus_tag="U2M88_000975"
     CDS             18479..19099
                     /locus_tag="U2M88_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEM8373370.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="U2M88_000976"
     CDS             complement(19191..20357)
                     /locus_tag="U2M88_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEM8373371.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="U2M88_000977"
     CDS             complement(20369..20722)
                     /locus_tag="U2M88_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEM8373372.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="U2M88_000978"
     CDS             20850..21872
                     /locus_tag="U2M88_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEM8373373.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="U2M88_000979"
     CDS             21891..22052
                     /locus_tag="U2M88_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373374.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="U2M88_000980"
     CDS             complement(22311..22745)
                     /locus_tag="U2M88_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373375.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="U2M88_000981"
     CDS             complement(22855..24714)
                     /locus_tag="U2M88_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEM8373376.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="U2M88_000982"
     CDS             complement(24832..25992)
                     /locus_tag="U2M88_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HEM8373377.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="U2M88_000983"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="U2M88_000983"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEM8373378.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="U2M88_000984"
     CDS             complement(27739..28437)
                     /locus_tag="U2M88_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEM8373379.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="U2M88_000985"
     CDS             complement(28394..30055)
                     /locus_tag="U2M88_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEM8373380.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="U2M88_000986"
     CDS             30273..31673
                     /locus_tag="U2M88_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEM8373381.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="U2M88_000987"
     CDS             complement(31925..33004)
                     /locus_tag="U2M88_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM8373382.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="U2M88_000988"
     CDS             complement(33090..34049)
                     /locus_tag="U2M88_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEM8373383.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="U2M88_000989"
     CDS             complement(34061..35572)
                     /locus_tag="U2M88_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEM8373384.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="U2M88_000990"
     CDS             complement(35594..36157)
                     /locus_tag="U2M88_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEM8373385.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="U2M88_000991"
     CDS             complement(36163..37026)
                     /locus_tag="U2M88_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM8373386.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="U2M88_000992"
     CDS             complement(37040..37936)
                     /locus_tag="U2M88_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8373387.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="U2M88_000993"
     CDS             complement(38111..39400)
                     /locus_tag="U2M88_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM8373388.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="U2M88_000994"
     CDS             complement(39372..40889)
                     /locus_tag="U2M88_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEM8373389.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="U2M88_000995"
     CDS             41087..41977
                     /locus_tag="U2M88_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEM8373390.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="U2M88_000996"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="U2M88_000996"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEM8373391.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="U2M88_000997"
     CDS             complement(42886..44358)
                     /locus_tag="U2M88_000997"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEM8373392.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44995..45357)
                     /locus_tag="U2M88_000998"
     CDS             complement(44995..45357)
                     /locus_tag="U2M88_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEM8373393.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45580..46332
                     /locus_tag="U2M88_000999"
     CDS             45580..46332
                     /locus_tag="U2M88_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEM8373394.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46472..47395)
                     /locus_tag="U2M88_001000"
     CDS             complement(46472..47395)
                     /locus_tag="U2M88_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8373395.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47410..47682)
                     /locus_tag="U2M88_001001"
     CDS             complement(47410..47682)
                     /locus_tag="U2M88_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373396.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        13931 a         9944 c         8259 g        15720 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttaatat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacattt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata tcagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggattaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgtttttt
    44941 attgtataca ttagatatgg agcaggttac cataaagatg attattagtc gttattaaat
    45001 agaatccttc gtattttttt gttctatcac tacaatatct ttatttggac gtttgaattg
    45061 tattccggca cgttctagct cctgtttcac tcctacatgg actttgacat cttctcgtgt
    45121 catttttaaa acgtgatgaa tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt
    45181 attgatcatc acgacttgct ttttatcata tgtgtctacc aatagcagtt gtgttcgatc
    45241 taacgaaaat aatttgtaat aattaatagc cgtattccca aaaaagaatt ccactacttt
    45301 aggataaaat gtcttaatat ttaaattact attcacaggt gtttcgatta gcttcatctc
    45361 attcctcttt tctatatagt cgatttagat ccaatcttaa gttatgttac catctttcct
    45421 agcattaagg aatcggttac aattgaacct ttaaagacaa aaggatttac cttttatcac
    45481 ttaatagtat aacataattt cgtgagaaaa aaatacaatt ttttcttgac catctcgtta
    45541 cgtaaaggat tatgcttaca tttgtcagga ggaaaaataa tggagtacac aataaagaaa
    45601 atgtcagaga tatcaggagt cagtccgaga accttgcgct tttatgatga gattggactg
    45661 ctaaaaccag ctaggatcaa ttcttcaggt tatcggattt atggcaaaaa agaagtcgat
    45721 cgtctgcaac acatcttatt ttatcgaaca atggcattca agttggatga tatccaagag
    45781 gtcttagaca atccatcttt tgaccaccag aaagctttaa tcaagcatca gcaaatgttg
    45841 ctggaaaaac gggcacaaat cgataccctc ttgacaactg tccaacagac acttgatatg
    45901 tacgaaggag gaagaaaaat gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag
    45961 ttgaaagaaa atgaagaatc ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag
    46021 gttacagcat ccaatgaaaa atggatgaat atggataaag aaacgtatga agaaatgcaa
    46081 actgttgaaa agcagatgtt atttgatctc actctttact taaaagaacc agaagatgaa
    46141 agcttagcag accgtatctt ccaagctcat aagaagtggt tctcttacag ctggcctgaa
    46201 tatcagccaa aagcgcataa aggactagga atgatgtacg aagccgatga acggttcact
    46261 gcatattatg acgagcgaag tggtcatggt gcagcaaaag ctttaaatga aattatccaa
    46321 aaaaaggcgt aatcttttac atgatccaac aaaaaagtac acgtaaaatg acctaataac
    46381 aaaacagcag acacccaagt tagattttta tgctaacttt tggtgtctgc tccattcttt
    46441 cacaagtttt ttcatcctac tttaaatata ttcatcgttg aataaacgta taaggtattt
    46501 tcactttatc ctttttttct gtaagaaaca attgaaatgc catttctccg caagcttcca
    46561 gatggtggtc gatggttggg aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa
    46621 tcaccactgg tgtcttatct tgcagatagc tttgcaagat gcctgctgca accatatccg
    46681 aatttgtaaa gacaccatca actccaagct ctttaaaaaa gaagcctgct tgttttcctt
    46741 tctctgcatc tatacaatcc caaaaaacaa gtgattcatc aaaagatgga aaaagttgat
    46801 gacatagctg caaagtgatt tttgagttat aacttagcct gccacttcgc cccaacgtga
    46861 tgcccaattg ctttatacct tgctgcttca aataagtaag accttctgta attgattgtt
    46921 ctcgatcaat aaagacacag cttgcctgtt ttcccttgat ttcttcacaa aaaactagag
    46981 gtccatattt ttgatatgcc aataaatcat ctagtgtatt ggcacgagac gtaatgatca
    47041 atccatcgaa agctttagct gcaaattctt caagatacct tcgctctttt tctttatgat
    47101 agcccgtcgg taaaagtgtc actttgtaat cataatcaaa cgctgcttca atgatcccac
    47161 tgatcaactg atcaaaatac ggatgattgg tataaggcag aatcactcca atgcttttcg
    47221 tttttccata acttaactgt ctggcatttg cattagggat ataatcgact tctttcatga
    47281 ccgcaagtat ttctttcctt ttttcctcat ctacatatgg atgtccattg attacacgtg
    47341 aaatagtaga aactgaataa cctgtgagct ttgcgatatc tcgaatgttt gccactgctg
    47401 cttcgccacc tatacaaaaa gattttcttg taaatagtct accacagctg gaccgcattc
    47461 aaaaaactga tacatttttc cttcatgcga aaaactgatc cgctgcccgc cccaatatat
    47521 atcttttgtc aaattttcga tttcattcat tggtatttct tttactatat tcctattcaa
    47581 cccttgaaaa ctgtacaacg ttaatacgga tcccttgaac tcgacaaacg cataaaaata
    47641 attgttcgtt gtgccatctg ttatctctac ataagtattc attgcaagcc ctccttttga
    47701 tactttgagt atagtgtatg aaacgcgtct catagcaagg agtttgtttt gccaaagaaa
    47761 agttttattc ctctttcatt attaccaaag tctcttattt taaattgaat acattataat
    47821 gatactgtaa atatatcttt attattagtg aatg
//
LOCUS       DASHRQ010000016        47419 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.016, whole genome shotgun sequence.
ACCESSION   DASHRQ010000016 DASHRQ010000000
VERSION     DASHRQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.016"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(250..2325)
                     /locus_tag="U2M88_001002"
     CDS             complement(250..2325)
                     /locus_tag="U2M88_001002"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEM8373397.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDYEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTYHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDADRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2327..3244)
                     /gene="glyQ"
                     /locus_tag="U2M88_001003"
     CDS             complement(2327..3244)
                     /gene="glyQ"
                     /locus_tag="U2M88_001003"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEM8373398.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3630..4442)
                     /gene="recO"
                     /locus_tag="U2M88_001004"
     CDS             complement(3630..4442)
                     /gene="recO"
                     /locus_tag="U2M88_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEM8373399.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKDMSEKDLPKKEE"
     gene            complement(4585..5484)
                     /gene="era"
                     /locus_tag="U2M88_001005"
     CDS             complement(4585..5484)
                     /gene="era"
                     /locus_tag="U2M88_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEM8373400.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5499..5894)
                     /locus_tag="U2M88_001006"
     CDS             complement(5499..5894)
                     /locus_tag="U2M88_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEM8373401.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIVGLILFVPKIYHLLFN"
     gene            complement(5878..6354)
                     /gene="ybeY"
                     /locus_tag="U2M88_001007"
     CDS             complement(5878..6354)
                     /gene="ybeY"
                     /locus_tag="U2M88_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEM8373402.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6383..8575)
                     /locus_tag="U2M88_001008"
     CDS             complement(6383..8575)
                     /locus_tag="U2M88_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEM8373403.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLFAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8599..9570)
                     /locus_tag="U2M88_001009"
     CDS             complement(8599..9570)
                     /locus_tag="U2M88_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEM8373404.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10276..11655
                     /gene="brnQ"
                     /locus_tag="U2M88_001010"
     CDS             10276..11655
                     /gene="brnQ"
                     /locus_tag="U2M88_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEM8373405.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGTNWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11811..12257)
                     /locus_tag="U2M88_001011"
     CDS             complement(11811..12257)
                     /locus_tag="U2M88_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEM8373406.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12284..12460)
                     /locus_tag="U2M88_001012"
     CDS             complement(12284..12460)
                     /locus_tag="U2M88_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEM8373407.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12622..13050)
                     /locus_tag="U2M88_001013"
     CDS             complement(12622..13050)
                     /locus_tag="U2M88_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEM8373408.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13142..14002
                     /locus_tag="U2M88_001014"
     CDS             13142..14002
                     /locus_tag="U2M88_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEM8373409.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(14097..14447)
                     /locus_tag="U2M88_001015"
     CDS             complement(14097..14447)
                     /locus_tag="U2M88_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEM8373410.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14463..16190)
                     /locus_tag="U2M88_001016"
     CDS             complement(14463..16190)
                     /locus_tag="U2M88_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEM8373411.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIQKMTEE"
     gene            complement(16404..17330)
                     /gene="trxB"
                     /locus_tag="U2M88_001017"
     CDS             complement(16404..17330)
                     /gene="trxB"
                     /locus_tag="U2M88_001017"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEM8373412.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17475..18911)
                     /locus_tag="U2M88_001018"
     CDS             complement(17475..18911)
                     /locus_tag="U2M88_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEM8373413.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(18908..20311)
                     /locus_tag="U2M88_001019"
     CDS             complement(18908..20311)
                     /locus_tag="U2M88_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEM8373414.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARINDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            20541..21443
                     /locus_tag="U2M88_001020"
     CDS             20541..21443
                     /locus_tag="U2M88_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEM8373415.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(21484..23370)
                     /locus_tag="U2M88_001021"
     CDS             complement(21484..23370)
                     /locus_tag="U2M88_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM8373416.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIRAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILMMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGNLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKTSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFKSL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(23466..24317)
                     /locus_tag="U2M88_001022"
     CDS             complement(23466..24317)
                     /locus_tag="U2M88_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEM8373417.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSEQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGEKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(24774..26558)
                     /locus_tag="U2M88_001023"
     CDS             complement(24774..26558)
                     /locus_tag="U2M88_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8373418.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEKYAPQQNPGADQTITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(26558..28309)
                     /locus_tag="U2M88_001024"
     CDS             complement(26558..28309)
                     /locus_tag="U2M88_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8373419.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFVIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(28451..28675)
                     /locus_tag="U2M88_001025"
     CDS             complement(28451..28675)
                     /locus_tag="U2M88_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEM8373420.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            28926..30512
                     /locus_tag="U2M88_001026"
     CDS             28926..30512
                     /locus_tag="U2M88_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEM8373421.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            complement(30822..32597)
                     /locus_tag="U2M88_001027"
     CDS             complement(30822..32597)
                     /locus_tag="U2M88_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEM8373422.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSRRKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(32808..33710)
                     /locus_tag="U2M88_001028"
     CDS             complement(32808..33710)
                     /locus_tag="U2M88_001028"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEM8373423.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEGGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQAFDPEVFPELLAEEVTL"
     gene            33989..36334
                     /locus_tag="U2M88_001029"
     CDS             33989..36334
                     /locus_tag="U2M88_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEM8373424.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKVIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            complement(36497..36763)
                     /locus_tag="U2M88_001030"
     CDS             complement(36497..36763)
                     /locus_tag="U2M88_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373425.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNHQ
                     MTAEEAAQEFSNRHVHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(36756..37079)
                     /locus_tag="U2M88_001031"
     CDS             complement(36756..37079)
                     /locus_tag="U2M88_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373426.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            complement(37091..37297)
                     /locus_tag="U2M88_001032"
     CDS             complement(37091..37297)
                     /locus_tag="U2M88_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373427.1"
                     /translation="MTEQEIIEALASVVATKENLVDSAKEVYLLRINKARRMGEAFDT
                     LVKEIQDKINEIVTRDRELAQQFN"
     gene            complement(37294..38349)
                     /locus_tag="U2M88_001033"
     CDS             complement(37294..38349)
                     /locus_tag="U2M88_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEM8373428.1"
                     /translation="MMADKQYSVISEAVYWLDPKHRDYDHTYKENSIKKIDSYNFKIL
                     KIKDSLDGMQAMGVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRPG
                     ASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALYV
                     AAEKQWKNVGLNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEVR
                     ISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQEL
                     NNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELEK
                     KWHGKKACGKHVLSPLR"
     gene            complement(38349..38744)
                     /locus_tag="U2M88_001034"
     CDS             complement(38349..38744)
                     /locus_tag="U2M88_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373429.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGAYRMFVTMINNHDESVYFTFGFWKERNELGDY
                     GVENKSIQKEGITVNKIRVIFSNNKEEYI"
     gene            complement(38787..39041)
                     /locus_tag="U2M88_001035"
     CDS             complement(38787..39041)
                     /locus_tag="U2M88_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373430.1"
                     /translation="MTVKDEEYRYISEHVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKDSLDGMQAMAAAPIVHSKLEKNFKNKKIPTNFRVLK"
     gene            complement(39038..39433)
                     /locus_tag="U2M88_001036"
     CDS             complement(39038..39433)
                     /locus_tag="U2M88_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373431.1"
                     /translation="MPKKRRKTIVIILTILAIGIGGKFYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGCYGALVTMTNNEGKSVYFDYFFSKQMNEISSY
                     GVENEEIQNEGVTTPKIRVIYSNGEAEEV"
     gene            complement(39787..40788)
                     /gene="ccpA"
                     /locus_tag="U2M88_001037"
     CDS             complement(39787..40788)
                     /gene="ccpA"
                     /locus_tag="U2M88_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEM8373432.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            40998..42101
                     /locus_tag="U2M88_001038"
     CDS             40998..42101
                     /locus_tag="U2M88_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEM8373433.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGADSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVVWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            complement(42163..42765)
                     /locus_tag="U2M88_001039"
     CDS             complement(42163..42765)
                     /locus_tag="U2M88_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEM8373434.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSAELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(42768..43208)
                     /locus_tag="U2M88_001040"
     CDS             complement(42768..43208)
                     /locus_tag="U2M88_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEM8373435.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIIRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            complement(43334..44272)
                     /gene="galU"
                     /locus_tag="U2M88_001041"
     CDS             complement(43334..44272)
                     /gene="galU"
                     /locus_tag="U2M88_001041"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEM8373436.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKIELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            complement(44287..45312)
                     /locus_tag="U2M88_001042"
     CDS             complement(44287..45312)
                     /locus_tag="U2M88_001042"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEM8373437.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNHYFRIYTNPDVVGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            complement(45356..46186)
                     /locus_tag="U2M88_001043"
     CDS             complement(45356..46186)
                     /locus_tag="U2M88_001043"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEM8373438.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLLVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            complement(46201..47139)
                     /locus_tag="U2M88_001044"
     CDS             complement(46201..47139)
                     /locus_tag="U2M88_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEM8373439.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            complement(47276..>47419)
                     /locus_tag="U2M88_001045"
     CDS             complement(47276..>47419)
                     /locus_tag="U2M88_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM8373440.1"
                     /translation="DFREVNQTFISSVSNQRNYIPRKSLNYRTPIEIFLSYVQEAFYS
                     SLI"
BASE COUNT        13262 a        10091 c         8469 g        15597 t
ORIGIN      
        1 taatcatacc acaaactgct ttcttgacta gggaaattta cgatttaaaa gtaaataaaa
       61 cattaataag ataacaatta atcgatttta cttttatttc cttaattttc ataggtgttt
      121 ttaaaagtta cacaaaaaat aaaataacaa aaaaaattaa cattgaatta ataatagtta
      181 tttataacaa ataaaccaga gaaatagcct attttgtgcc tgttctctgg tttatttaca
      241 tattagaaat tattttgtga tgatttgatc taaacttgct agtgacaaag ccatggcagc
      301 aatttgactt aattgcgtta atcggttctt acgtactgct ggttcttctg ccatgaccat
      361 cgtctgatca aaataatttt cgatcagtgg gcgaaggctt acaagctgat cataattttc
      421 gttgatcgtt tggtcagcaa agcttgtttt cattgcctct actgctttgt gcagttcttt
      481 ctccgcttcg ttttcgaaca gttcagggtc tacttctgat tgtttttcaa gctccgcttt
      541 tttcgctaag ttcacgacac gtgtcaaagc ttccatcgat ggtttgaagt cttcatctgc
      601 aagatgctgc ttgaacaatt gtgctgctgc aaatactttt gtcagatctt tttgttctgc
      661 attcaaaaca gcttcgatga catcatgacg aatattcttc gttgtcaata attggcgcag
      721 acggcctttg ataaagtcga tcacctgttg ttgtcccgct gacaagccaa tgccaaaacg
      781 atccgcatct ttactgataa cttcactaat cgtttcttgc agaacagata aaggaaaggc
      841 ccattttttc gattcgatga ttcgaacgac accatatgct tgacggcgca aagcatatgg
      901 atcgtttgag ccacttggga tcatcccaac tgcaaagaaa gaaaagatac tatctaattt
      961 atcagcaatc gctaaaacag aacctactgt agtctcaggt agatctcctt cactggatat
     1021 aggcatgtaa tgttcacgga ttgctgttgc tacggctggt ttctcgcctt taagcagagc
     1081 atatttttct cccatgatcc cttgtagttc agggaattct cctaccatgt tggttactag
     1141 atcaaattta tagatttcag cagcgcgttg cagatccatc aattcttcct cagatagtcc
     1201 tacttgctcg cctatgatgc gggcgatgac ttgtacccgt tgcattttct cgtagatgct
     1261 accgattttt tcatggtaag taacagtttt taatttttca acgtattggt cgattgaagc
     1321 tttcttgtct tcattataaa agaattccgc atcttctaaa cgagcagtca atactttttg
     1381 attgcctcga accacatttt ccaattttac ccgatcacca ttacgagcag aaataaaatg
     1441 aggaagcagc ataccttgat cattccgtac ctcgaaataa cgttgatgtt ctttcataga
     1501 agtgactagt acttcttcag gaacagaaag atatttttct tcgtagtcgc cagaaaagac
     1561 agttggatat tcgactaaat tgttgacttc ttctagtaaa tcttcgtcta gatcaataac
     1621 ccagtgattt ttttctgcta attcgtttgc ttgtgtaacg atcatttctt gtcttttttg
     1681 cggagacgca atgacgaatt gttcattcaa tttttcttca tattcttttg catgcgcaaa
     1741 tgtcacatct tcgcctaaga aacgatgacc tcgagaggct ctgcctgttt tgacatccaa
     1801 gatttcgaat gggattactt catcatctaa caatgccacg atccagtgaa tagggcggat
     1861 atattcaaaa tcgtattctg cccaatgcat cgttacaggg aaagtcaggc ttttaatgac
     1921 atctgctaaa cctgtcaata cttctttgga ttccttacca tgagtgtatt tcgttacata
     1981 gacatattcc acacctttta attctctaaa agtgatagcg tctgtcgtca gaccttgtcc
     2041 gcggacaaat ccttccgctg ctttcgtcca gtttccatct gcgtccaaag caattttttt
     2101 ggctggtcct ttgacttctt cttcactgtc cgcttgtcgt tctggaatat ttgttacttt
     2161 gattgctaaa cggcgcggtg tggaaaatgt ttcgatcgta tcataagtca attggttttc
     2221 atctaaaaat tttgctgttt tttcttctaa ttgtttcatg cttggtgtga caacatgggc
     2281 aggcatttct tccagcccga tttcaagtaa taagtctttt gccatcttat tcagcctcct
     2341 tcacggtttc tggatttgct gtgtttttat tcaacaaagg gaatcctaat ttttcgcgtt
     2401 ctgccacaaa gattttggct acagcacgag ccatgttgcg gatacgggaa agataaccgg
     2461 cacgctctgt tacggatact gcaccgcgag catctaataa attgaatgta tggctgcatt
     2521 tcaaaatata atcatacgct ggatgaacaa gattttcatc gatacagcgt ttagcttctt
     2581 tttcaaaacg atcaaagttg cttagtagca agtcttgatc gcttacttca aaagaatatt
     2641 tggaatgttc atattctggt tgtttgaaga tctcgccata tttcacgcca tcagcccatt
     2701 ctaaatcata gacactttca acttcttgga tataagaagc aagacgttct agtccataag
     2761 tgatctcaga agtgactggt ttacaaggca gtccaccaac ttgctggaag tacgtgaatt
     2821 gtgtgatttc cattccgtca agccatactt cccaacccac accggcacag cccatggatg
     2881 ggttctccca gttgtcttca acaaaacgga tgtcgtgttc taatggatcg attcctaaaa
     2941 ggcgcaagct ttctaaataa agctcttgga tattctcagg tgaaggtttc atcacgactt
     3001 ggaattgatg atgctgatac aaacggtttg ggttttcacc ataacgtcca tcagctggtc
     3061 ggcgagatgg ttctacgtaa gcagcattcc atggttctgg tccgattgcc cgtaaaaagg
     3121 tatatgggct catcgtacct gctccttttt ctgtgtcata cgcttgcatc agcatgcagc
     3181 cgtttgatga ccaaaatttt tgtaatgtta aaatcatatc ttggacggtc aattttttac
     3241 tcatctgttt tgcccctttc aattttaaag cgttgctgtc aaataagaat acaggaaaga
     3301 aaacgacaaa aaatccctat gtctgattac agacataggg acgaataatt cgcggttcca
     3361 ccctacttcc gaataaagat tcggcagctt catttatcgt actcgcaagt gcctcttcaa
     3421 aaatcgtttg ggtccggctc ccactctccc gaactcgcta aaccacgtgt tttttactga
     3481 tcttggtcaa tgtacgtatt cattttgcag ttttcttgtt tctataatag atcctctcac
     3541 tgaacgatct atttgatcta cggttaataa tagacggaac tccctgtgtt tgtcaaatgg
     3601 ttttgatttt ttgttgtatg tttcatttac tattcttctt ttttaggtag atctttttca
     3661 gacatgtctt tctctggcgt ctttaatacg tgtgcccaag acttcatttg atcgatgaac
     3721 tttttgcttt tcaaatgaat accgacatat tcatcataca gctggtcgat gagttcacgg
     3781 attgcttgtt tggtttcttc tttcagatgg attccactga ttttatcata agaaatagct
     3841 gaaaacatcc gaacgaaata aacagctcga ggatctgcat gataacggtg atcgtccatc
     3901 gaccaatgtc tttcgcatat cacaccacca tatttggaag aatagtcaaa ttttcctttt
     3961 gtttccccac atacgacgca gtgtgtccag attggtgcaa ttccgaagcg ttgcagtatt
     4021 tgaacttcaa agatattcgt gatgatttct gcatcatctc cttgatccat ccgctgcaga
     4081 gcttgttgcg taaattggaa taaatgcggg tcatattgat gatcttcgat tgctgcatct
     4141 actagattta gcaaataggt accataaccg ctcaaaaaga tatcttcttg gatgttccga
     4201 aacgcatgga cgtctttgac actgcttaaa aacgacaaac cttcacttcg aaaattaccg
     4261 atatagactg cttcggtaaa ggggagcaaa gccggagcta atggctgatt ttgccgatgg
     4321 attcctttga tataaaacat cattttgcca gaagattccg taaagatttt gactaattta
     4381 tctttttctt tgtaattacg gctagataaa atcaagcctt tcgattctgc aagctggctc
     4441 atattctccc tcctaaaaaa taaatctgga aaagcagatt ctgccgatcc agattattta
     4501 tgtttcgatc ttccgccgtt tttctcaacg tgcggatttt tatccagttt atttctatac
     4561 ttaacctata agaagttcag aatatcagta ttcatctttt cggtagccga agtcttgcag
     4621 atagactttt ttgtctcgcc agtctttttg gactttgacc caaagttcta gaaatacttt
     4681 gtctcctaaa agattttcga tatcctttct tgctttcgtt cctacttgct tcagcatttt
     4741 tccgcctttg ccgataatga ttcctttttg gctatctctt tcgacgatga tcgtggcctg
     4801 gatatggatt ttatcattct cgtttcgctt catcgaatca acaacgactg cgacagaatg
     4861 aggcacttcg tctcgtgtta aaaacaagac tttctcacgg atcaattcag aaacgatgaa
     4921 gtattcagga tgatcagtca cttgatcatc tggaaaatat tgtgggccct ctggcatttc
     4981 actgactaat acatccatca aacgttcgac gttgtttcct tctgtagcag aaattggaat
     5041 cacttgtgca aagggcatct gcgttgaata atcttcgatg attcccaaca actcgtctgg
     5101 atggatttta tcgattttat tgataatcag ataaacagga gaatgcacgt tttttagacg
     5161 ttcaataata aaatcgtctc cccggccgcg tttttgatca gcgctgatca taaataagac
     5221 cgcgtccact tctttcaacg cactgtaagc tgcttctacc ataaaatctc ctaaacgatg
     5281 ctttggttta tggattcctg gtgtgtcaat aaaaatcaac tgagcttctg gtgtagtata
     5341 tactccttgg attttatttc tagttgtctg cgccttatca ctcatgatgg caattttttg
     5401 acccacgatt cgattcaata atgtggattt tcctacattc gggcggccga cgattgcgac
     5461 aaaccctgat ttatgttctg gcatatacgt tctccttttt aattaaacaa caaatgatat
     5521 attttaggga caaataaaat caacccaacg atgacggcaa aacaagcggt cactaaaact
     5581 gctccagctg ccatatcttt gatttttttc ccaatcggat gaaaatgaaa atccgtaaac
     5641 atgtctacga cattttcaaa aacagtattg atgatctcta ctatccatac gagaaaaaca
     5701 gctaaaagca gccataacca ttctatccga tcaagttgga aaatcacgcc cataaacaaa
     5761 gcgaggattc cgaacgcaac atgtttcctc atgttgcgtt cttcctcaaa aacagttttg
     5821 actccttgga cggcaaattc cacggacatc aggaaatgtt tgtttttctc tacgttttta
     5881 tcttttaagt ccataggcat ctaagatctc tttctgtaaa ccaaacattt ctttctcatc
     5941 ctctggtgtc atatggtcat acccgttgat atgcaagaat ccatgaaccg ctaaaaagcc
     6001 aagttcccga tcatacgaat ggccgtaagc ttctgcttgc tctgcagcac gttcagtaga
     6061 gatcatgatg tctcccagta cacgaggcaa tggattctct ccatcttcaa aaatcactgg
     6121 gatttcgtct tcaccctctt cttccaaggc aaaactgatg acgtctgtcg gtgcatcttt
     6181 tccacggtaa tcacgattga ttacttggat cgctgcgttg tccatgaaag taacagacat
     6241 ttctgtttct tctggcaact ctaaatatga tgctgcaaat tgcaacaagt cgtcgatttc
     6301 tttcattttc tcttctgaga cttggtttgt ttcatccatg aaggtaatat ccattataca
     6361 tcgcttcttt ctattcttct tgctattctt cttgttcttc ttttatcttc tcaacttccg
     6421 atttcatttt aggatatttg atccgagaat ggaaggtact gcttaaaccg ctgatcaatg
     6481 atttttctac tttacggatt tcttttaacg tcaatccact gtcatccaat tgtccatcgg
     6541 aaatacgctc ttctataaga ttatggacaa aactttcgat tttttcgctt gttggatggt
     6601 ccattgcccg tactgccgct tcacaactgt cagctatatt gacgattcct gcttcacggc
     6661 tttgaggttt tggtccagga taacggaatt gctcttccgt gacttccggg ttgcgttcct
     6721 ttgccttaac gtagaaataa cgcatcagcg tggttccatg atgctgtctg caaatatcga
     6781 tcaccatttg cggcatatta taatcttcca atattttggc accatctgtc acatgtccaa
     6841 agataatatg tttactgtct tctggcaata agaaattgtg cggattttcc gctcctgaag
     6901 gcaagttctc aacgaaaaag ctggcgtgtt tgatcttgcc gatatcatga taatagcaag
     6961 ctactcgagt tagtaaagaa cgtccaccga tttctgctac agcattagca cttagattgg
     7021 ctaccatcat gctatgatga tacgtacctg gtgcttcttc taacaactgt ttcaataaag
     7081 ggtgattagg attgctgagt tcatttaata cgatcacact atcatcattg accatcaatt
     7141 caatataggg gtgcagacct atcgtcagca agaatgagaa caatgcacct gtgaatccgc
     7201 agactagcgt taaccaagtc gttgcatccc caaagctcat tccttggtag ataatcaaaa
     7261 tgacatccat catcacaggg aaaataatga cccacatagc agcgaataac ccttgttctg
     7321 agatccgttt gcgtctgacg atcgttgcca tgagaccaga aaatacatag gacatcaaaa
     7381 taactgtcag aaagttcgtg ccaatcgaat cataaaaaat gaaaagtgcg gatactgctt
     7441 ggaaaatacc agccatgatc cctgctcttc gattcaagaa aaagcttaaa acaagtggag
     7501 caaaagccgc ggggtaaaac agcggaatat aagcagcttg ttctgtttgg aagagttgga
     7561 aaaatttcat tagtactacg ctgactgaca ttgctgtcac gtagaaaagt acgtattttg
     7621 ttctttctcc tgcattttga aattgaagtg aattataaat cagtacaccg atttgaagca
     7681 aaacagctaa aaccatggca atgagtggga aaatcgaagt cgtttgattc gtcaatccta
     7741 gtaattccaa ttttttcatc gctgaagcat caatttgatt tccttcgcga acaatgattt
     7801 ctccttggaa gatcatcact ggttgtacag cttctcgtgc agattgtttt aattcttctg
     7861 tctttttctc attcaataca tcattgacta caatgccttg atccactaag tagcggatca
     7921 tttgttgctg ctctgttgtg acattcaaat actgaatctg atcttctgct tcttgtttaa
     7981 aagcttccaa ttcgctttca cgaacttgtt tactcatctg ttcgtcaatg agcttcaaac
     8041 tttcatccct aaccgtgtga atttccgttt cagtcatttc aaatatcgtc gaataaaaat
     8101 tgttcggcag tttttggtaa aacgcaacgt tttctgcatt cacgttttca aaatttttct
     8161 tcagtgtagc aatccgctct tctgtagtag gtttcgggat attttcatct tccttcgcct
     8221 tatctacctt gtcattataa gatttgttga tggtatcatt cgtcttatcg atcaattcga
     8281 ataattgctt gatccgattg ttctggtcat ctgccagatc tttttgataa gtatattctg
     8341 gagtcactgc ttctgctgct agttttcttc tttgttcggt ttcttccgtg ttctcgatcg
     8401 ttttattcgc acggatactc tcttctgcta cttggccttc tctaaaatca ttggcctttt
     8461 gtttgacact actaaatgta acgccgcaaa caatgataaa aaacaagaac agaaggaaag
     8521 gcaatgctgc ctttccgagt ttttcttgta gtttctgcag tgttttattc aacatgtgct
     8581 tatcctccta agcaaagctt attcttgatg aagatccgcc ttttcataag ctttgatgat
     8641 ttcggcaact acagggtgcc gaacaacgtc acttgcttca aaattaacaa aatcgatttt
     8701 tttgatttgc tttagtgttc gttctgcatg aacaagacca cttgtcgtcc ctcgaggcaa
     8761 gtctatctga cttgtatccc cattgacaat catttttgat tgataaccga gacgggtcaa
     8821 gaacattttc atctgagcga ctgtcgtgtt ttgtgcttcg tccaagataa cgaatgcatc
     8881 atccaatgtc cgcccccgca tgtaagctaa tggtgcgatt tcgattacgc ctcgttccat
     8941 caatcggttc gtatgatcca atccaaaaat ttggtataaa gcatcataaa caggccgcag
     9001 ataaggatcc actttttctt tcagatcccc tggtagaaag cctaggtttt cccctgcctc
     9061 tacagcgggc cgcgtcaaaa tgattttttg cacttcgcct ttttttaagg cagcaattgc
     9121 taatactacg gcaagaaacg ttttccctgt tccggctggc cctacaccaa atacgatgtc
     9181 atgtttggct accgattcaa catatttctt ttgaccgctg ttttttaccc gtataggttt
     9241 tccattacgg tctttgacga tctcttcctc atacatctca acaaaataat ctaatgtacc
     9301 tttttgcccc attttcaatg ccgtcacaac atcgggcgta cttacgtgga ttcctcgttt
     9361 gatcaattcc tgtaatgtcc gaatgactga ggcagccaag gaaatctggg attgttcacc
     9421 aatgatctga atgatttctc cacgtgtatg gatggttgtt tccgttgttt cctcgatcag
     9481 ttttatatgc ttgtcgtgtg cacctaaaag catgctgaca tcatcagcag ctgtcaatct
     9541 aatctctaaa gaactagact cttccgtcaa aaatagacat cccctttttt tcgattcatt
     9601 ctttcctatt ttaccatact cagaagaaac tatatagcaa ataagaaaag gttgtaaaag
     9661 agtcgtttaa ccatacgtac caagaaacgt taaaaaatag ccgtttccat tcgctaaagg
     9721 tctcgttttg ataaaaagaa tctgagaatg taagttatat gaaagtggag aacaaaatat
     9781 tccgtttcat tcagcaggta ttctctaggt atttttcttg tttcaaaaaa agaaaacaaa
     9841 atagttaaaa tattcttgca tttcaaaaaa aacaggtgta ttattacgtc aacaaataaa
     9901 catacagcaa agcgtcgagg aaaacagtag gctattcaac gatttattcc cagtgagtca
     9961 tgtaagtgag aaatgacaat aaatcgaata gacgaacatc atttccgagt caatgtcgaa
    10021 aacgtagatc attgtggctg cctccattat caggctagcg gataacttta tcaaaaagcg
    10081 tccgtgaatg aagaagcttt tttaagcgaa aaaaggtggt accgttctag aatctagagc
    10141 ccttttccca agtggaaaag ggcttttttt atattttaag gtggtggttt tcatggaatc
    10201 tgatattaat ggcattacta attgctgcaa cgtttatatc gtcaatcaaa atgataatta
    10261 ttggaggaaa aatttatgga agaaacaaaa acttattcat ggaaacaact attgatggtc
    10321 agttcactta tcttcgggat gttcttcggg tcaggtaatc taatctttcc tgtccactta
    10381 ggtcaaatgg ctggcacaaa ctgggtcagt gctggcgtag gatttgccat ctcaggcgct
    10441 cttttcccat tactagctat cttagctgta gtagtgacaa aaagtgatgg tctgtatgac
    10501 ttagctaaac ctgtcggaaa atggtatgct gccttgttct taatccttgt tcatttgacg
    10561 atcggaccat ttttcggtac accacggaca gctgctactg cttttgaaat ggctgctaaa
    10621 ccatttttgc ctgaaaaata taattcatta ggtatgtttt tattctctgc tcttttcttt
    10681 ttacttgctt atttccttac tgtccatccc acacggttgg tcaaatacgt cggaaagtat
    10741 ctaaactcct tgtttcttgc attattagca atcatttttg tcgtttcttt attccaccca
    10801 atgggaaatc taaatcaatc tgctgcagat acttaccaaa caaacgcttt aacaaatggt
    10861 gtcctagaag gttataacac ggttgatgca gtagctttgc ttgccttaag tgtcactttt
    10921 gtccatgcag tccgtgacct tggtttcaaa gataaaaaag tcacagaatt aacagcaaaa
    10981 gctggtagcc tgagcgttgc gctagaagtc atgatctatt ttggacttgt tcttctcggt
    11041 gcgatgagtt tgaataagat gtctttatca gaaaatggcg gaactgcact ttctcagatt
    11101 actaattttt atttaggtaa cttcggcgct agcttcttag gagtgatggt cacattaggt
    11161 gtcttcacta ccgctatggg actcgttgtt tcttttgcac aagatttcca taaactgttc
    11221 cctaaagtca gttatatgac atggctgcgg ttaacgactt tcgtctcttt tgtcgttgca
    11281 aatgctggtt tggacaatat catccaatgg tctttaccag tattgatgct tttgtaccca
    11341 ttgtcgcttg cgttgatctt gctttcctta acagcaaaat ttttccaaaa gacaccattt
    11401 gtctatcaag taacgatgtt gtttgctgct gttcccgccg tattggatat gctggcaaat
    11461 tcgcctgctt tagtgagtca acaaagagtt gtagcaagca tgctcgaatt ttatcatcat
    11521 cacgttcctt ttgctgcatt aggacttggc tggatggtcc caacgttact tggttacgct
    11581 ggcagcttac tcttctatta cgcctatcgc ctatcagggt acaaacaaga agcaaatgaa
    11641 ctaccagaag aataaccaga tcaaacacaa tttaacataa gaaatattaa acaggaaaaa
    11701 accagctaga tgaaagaatc ctattctttc gtctagctat tttttagatt tcttttttac
    11761 gagcacaaaa aaactgcgga caatgattgt ccgcagttta aatgataaaa ttaagattgt
    11821 aatagttctt tcacaatggc atttacttgg tttccatctg cttttccttt gactttaggc
    11881 atgatcgcac ccatgacttt gccaaattct ttcattgatg aagcaccagt ttgtgtgatt
    11941 gcttcttgaa caagttgtcg aatttcttcg tcggaaagtt gttccggcat gtatttttcg
    12001 actatcacaa tttcactttt tactttttca gctaaatcgt cacgtcctgc ttcttcaaac
    12061 tcatgcaaag aatcacgtcg ctgtttcatt tcgcgagaca acaccgttaa ttcttcttct
    12121 tctgtcaagt cgtgaccttc tttaatctgc tcgttttgta tagatgactt gatcattcgg
    12181 atgacttgta aagattcctt atctttcgct ttcatcgctg ttttcatgtc atcgtttaat
    12241 ctacttagta gtgacacact accaactcca attcatcaca gaactagaat tttttgcgtt
    12301 ttctagctgc ttcagatttt ttcttgcgtc ttacacttgg tttttcgtag aattcacgtt
    12361 tacgagattc ttgtaaagta cccgcttttg acacggaacg tttgaagcga cgaagagcat
    12421 cgtcaagtga ttcgtttttg cgaacgactg tttttgacat gttaattccc tccctccgag
    12481 cttaaaaagt aaccgtaatt gttattgaaa tatatctcaa aaaacaagac tgtcctgtaa
    12541 acattatatc taatgctgtt ttgaccgtca aggaaatctt gctttttttt aaaattatca
    12601 aatgttattt tatttaaatc cttattgaca attttcacat cttccgaaaa tctcgaatct
    12661 atgcccttcg atcgtacatc caccgagctg ttcttcgaaa taattcattg gacacatatg
    12721 gatttctttc gttgttccgc acaccgtaca gataaagtga tggtgatggc cgatttcccc
    12781 ttgacagcaa tgaaaacgaa acttcatctc tccatttaaa tcggtttctt ccagtaaatc
    12841 gatttctgca aaatcatgga gatttcgata aatcgtatcg taacttactc ctttgaattc
    12901 ctgattcata tagtcaaaaa cttctttagc ggatacgtag cgattgcgct tcactaaata
    12961 agttaacaat gcttctcttt ttttagtata tttaaaaccg ttatttttca atgtttctag
    13021 tgattgttcg atcaatgtct gttttttcat gacttattcc tccaaatcaa aatgattacg
    13081 aagtacacat tctagagtat ggtataataa acataaaaaa aatgcaagtg aggttttatc
    13141 catgtcttcc gattctacag aaatcgttac tttttttgtc atctccgact ctgctggaga
    13201 aactgcgaca aaattagcgc aagccactat ggcccaatat cctagcgttg agttcaattt
    13261 attccgcaga acatttgtga cagatgagaa aacattgatg caggcactgc aagatgcact
    13321 agaagaaaaa gctcttgtct tacacacatt gatcaatcaa gaattgatcg agatgacgcg
    13381 ttctttttgt gaagaacatc aattatttag ttttgatgtg atgactccgc cgatatcaga
    13441 aatcgagcgc ttaactggta tcaagcccat tcggcaacct ggcgcccttc atttattgaa
    13501 tgagaactat ttcaaacgga ttaaagcaat ggaatttgct gtaaaatacg atgacgggaa
    13561 agatcctcgt gggtttctag aagcagatgt cgttttgcta ggcgtatccc gaacttccaa
    13621 aacgcctttg agcttgtttt tagctaacaa aaatttaaaa gttgccaatc ttcctttgat
    13681 cccacaagca catattccca aacagctttg ggagattgat cctaaaaaaa tcgtgggatt
    13741 gacgaataac ccagatattt taaacaatat ccgtaaagaa cggatgcgct cttacggatt
    13801 aaatcctgat actgcttatt ctgatatcga aaaaatacga gctgaactcg attttgcaaa
    13861 tgatctttat gaaaaactgg gctgcgtagt aatcaatgtc gcttctttat ctattgaaga
    13921 aacagcttcg cttattttga atgctttaga cctagaagat catagttatt atggaactga
    13981 aaccaccgaa gaaacagact aacagttttt cttgtctttt tcttaaaaaa aactggactg
    14041 tgtcagcgaa gaagattcgt tgacacagtc cagtttgtct ctatttgcac ctatcgttat
    14101 tgatgttcca catgtttgag tgtctggttc aaaatatcca gtacgtgaga atcatcaaga
    14161 ctatacaaaa tcgcttttcc ctcacgatgg gacttgacga catggttttc tcttaataaa
    14221 cgcagttgat gcgatacagc agattgttcc atttccactg cttgggtgat tgctgtcaca
    14281 ttttgttccc cgtctttcag atacagcaga attttgagac gagtggggtc actcaacatt
    14341 ttgaatagtt ggctgacttt ctctactttt gttttttgcg tgtctggcaa tccattcacc
    14401 tccaacttga aaaaagactg acttctaaag tttaccactt tttccatcag tctcaagttt
    14461 ttttattctt ctgtcatctt ttggataatc gcttcataag cggctacttt ttcagctgcg
    14521 tcttctgaag agtttccttt tacaccgatg taaaatttga ttttcggctc agttcctgaa
    14581 ggacggatag cgatccagcc attttcttcc agcgtatact tcaatacatc agatggcggt
    14641 gtcgttagtt tttctacgtt tccttcacgg tctgttctcg tcaactcttt gaagtcttct
    14701 gtttggatga cttttgctcc ggcaaattct gttggtgctt cttcacggaa tttttccatc
    14761 aaagcagaga ttttcgcaga accttcttgt ccgctcattg tcacagaaat cgttttttct
    14821 gcgtagtagc cataggtttc aaaaatcgtt tgcaagccgt catacaacgt tttgccttgt
    14881 tttttatagt aagctgctac ttctgctaat aagagcaatg cttggatcgc gtctttgtcg
    14941 cgaacgaaag gtttaactaa ataaccgtaa ctttcttcga atccgaacat aaaagtatgg
    15001 gaatgatctt cttcaaattg ttgaattttt tcagcaatga atttgaatcc tgtcaataca
    15061 ttgaacatgg tagccccata gtgttccgca attgctgtag gtaattcgct agaaacgatc
    15121 gatttcaata ccgctgcgtt ttcaggtaaa gtgcctcgcg attggtgtgc ttccaaaata
    15181 tattgagcaa acagtgctcc gatttggttt ccggtcaata cttcatagga gccatttggc
    15241 aaacgaacgg ctgcacctaa acggtctgca tcaggatctg ttgcaatcag caaatcagcg
    15301 ccttctttct cgccaagtcg tatcgcatac tcaaaagcag agtgttcttc tggatttggt
    15361 gatttgactg tagtgaaatc tgggtctgcg acagcttgtt ctggaactag tacgaattgt
    15421 tcgaatccag cttgtttcaa cgcacgttcg cccaacattt ttcctgttcc gtgaagtggt
    15481 gtgtacacaa gtttcaaatc tttccccatt gtttccacca attcatgatc aatcgttact
    15541 gctttgattt cttctagata agcggcatcc acttcttctc cgatgatcgt gatcaatccg
    15601 ctatgttcag cttcttcgtc agataatact tcaactttta acggattgct gacttcacgg
    15661 acatatttcg tcaatgcatc tgcgtctgca ggaggcattt gtcccccatc ttcgccgtag
    15721 actttatatc cgttgtaagc tgctggattg tgggatgcag ttaccatgat tcccgcaaac
    15781 gtcttcaaat gacggactgc aaaagaaagc tctggtgttg gacgtagact ttcgaataca
    15841 taagatggga tattgtgatg tgctaatgtt ttggctgctt ccatggcaaa ttctggtgat
    15901 tgatggcgtg agtcataagc gatagcgaca cctcggcgtt ttgtttcttc gccttgtgta
    15961 tccatgaaac gtgcaagtcc ttctgttgct tgtcttaccg tatagatgtt cattcgattg
    16021 atcccaggcc ctaagattcc ccgcatgcct gcagtaccga attccaacgg tgcataaaaa
    16081 gcatcttcaa gtttttcagg atcttcaccc agatcattta attgtttttt taagttttct
    16141 tctaaatttt cttcatgtac ccattgttca tacacttgtt cccaagacat gaaaataaca
    16201 cctctttctg aaatatggtt cttaataagt atagcattca cttattagat ataaaagtat
    16261 gaggaccaaa aaacgcttaa atcaagaaag gattctttta tcaagcacgg ttgcaagtat
    16321 tcgcataaat cggattcagt agatttcacg cgaaaaaaaa gagtaaaaga acaattgaaa
    16381 ccgttcttcc actcttttta aacttatgca gttgtttctt ctaaagattc caagtattta
    16441 tacacttgtt gtccagcgat cccaccatca ccaacagcag tagtgatttg tcgcaaatct
    16501 ttttcacgaa catctccgat ggcaaaaacg cctggtatat tcgtcttcat ttcgttgtct
    16561 gttggaatcc agccagcttc gttcgtcaac cctgcttctc taaatggtgc agttaatgga
    16621 tccagtccaa catagatgaa tactccgtct gctgccaaat caaactcatt gccggttttg
    16681 acattacgtg ctttcacacc tgtcactacc atttcattcc caacgatttc gtcaacgact
    16741 gtatcccaca taaaggaaat tttttcattc gcaaatgcac ggtcttggat gatcttttgt
    16801 gcacgtaatt catctctacg gtggacgatg actacttcag aagcaaattg tgtcaaatag
    16861 atggcttctt ctactgctga gtctcctcct ccgatcacta ctagacgttt gttacggaaa
    16921 aacgcaccat cacaaactgc acagtaagaa acgccacgtc cagcaaattc ttcctcgcca
    16981 ggtacgccta atttacggtg gatacagcct gttgcgataa tgactgtttt tccttcgtaa
    17041 gttttatctt cacaaataac ttctttgtgg ctgccgtgat ccttgatatc ttgcacgatt
    17101 ccataagcat tttctgtccc aaatcttgaa acaccttcat acatcttata agctagatca
    17161 ggacccatga ttgaatcgaa accaggatag ttttcgattt ctgccgtatt gttcatttgg
    17221 ccgccagggg cgccttgttc gatcatcaat acggataagt ttgaacgtga agcatataaa
    17281 gcagcagtca tcccagcagg acctgcgcca ataacgatta catcatacat atgtagattc
    17341 tcctttactt aatcaagtaa gcatacttta cagccaatgc gaaaaaaagt ctattgttct
    17401 gcttgcgttt ctcgaatagc tttattatac tcgaaaaatc cgctgaaaaa caatagactt
    17461 ttataaatca ttgatcaatc attcgaaaga ttttctccat ttgaatcaat tactttttta
    17521 taccagttga atgatttctt cttcgaacgg gctaatgtgc catttccctc gttgtcacga
    17581 tcgacataaa tgaatccgta ccttttcttc atctcacctg taccagctga tactaaatca
    17641 atacatcccc aagtcgtata gcctaataga tcgactccat ctaattctac tgcatctttc
    17701 attgcttgga tatgtgctgc cagatagtcg attcgataat catcttccac ataaccattc
    17761 tcatcgggcg tatcgactgc acctaagccg ttttctacaa taaatagtgg tttttgataa
    17821 cgatcataaa tatcattcat cgttattctc aagcctaatg gatcaatttg ccaaccccat
    17881 tcacttgctt ccagatatgg atttttgaca gaagcaaaga tatttcctgc agtctgttcc
    17941 aagagttcgt tgtcagtagt cgaaactcga gaagaataat aagaaaagga gataaaatca
    18001 acagtatgtt ctttcagcaa tgcggcatcc ccaggttcca tttcgatctg aatgccattt
    18061 cgttccattt ctttcaatgc ataagcggga tattctcctc gtgactggac atcaatgaag
    18121 aaataatttt ctcgatctga ttttctagaa gcccaaacat cttcaggatt gcaagtgtat
    18181 ggataatatg ctcctgctgc cagcatacag cctactttgt tctccggatc tacttcatga
    18241 gcaatcttcg ttgcgatggc acttgcaacc aactcatgat gtgccgcttg atatttgatt
    18301 tgttcttcat tttctccttc ttcaaaatac agccctgcgc ccatgaatgg tgcatgaagg
    18361 atcatattga tctcattgaa tgtcaaccaa tatttgacaa gtcctttata gcgattgaag
    18421 atgactcggc acagattttc ataaaaacca acgagttctc tggaacgcca cgcaccatat
    18481 tctttgatca aatgcatcgg acaatcaaaa tgtgtaatcg tcacaagcgg ctcgatcccg
    18541 tacttttgac attctttaaa gacatcctca taaaatttca acccagattc atttggctct
    18601 ttttcatcgc ccattggaaa aatacgactc caagcaatgg acatccgata agtcttgaac
    18661 cccatttctg caaataaagc aatatcttcc ttataatgat gatacatatc gatcgcaatt
    18721 tttgctggat aaaaatgatt ttcatcaaat tcaaacattt tcttttttcc agtaatgaca
    18781 ggaaaacggt cttctccgat tggcacaacg tctacgttcg ctaacccacg tccgccttca
    18841 tcataaccgc cctcgcattg attggctgct gtagctccgc cccataaaaa atcttttctg
    18901 aatcccatca tacttcctcc attacatgtg tattgcttga taattctctt gccagttcat
    18961 cttggacttc gattccttct gcttttaacc ggctgattgt ttccttgaat tgcggtaaat
    19021 actgctgatg tgcaataaac aactcatcca ttatccgttt tgctgtcttt cctgatcgta
    19081 taagcggatt gatcgtaaac gcttgcaaag ctgttccata atcaccagtc acggctgctt
    19141 cgatcgtcag caattccata ttcttcatta cttgcaacca gcctctttca gcagcaggaa
    19201 gggaaccaaa cgctacattt cgtgcgcctt gtgcgccaac ataggcagtg acttctacta
    19261 cacaatctgc cggcagatca ggaacagctc catcattctt tgtagatacc acgatctgtg
    19321 tttctttatt tccatagatc gaagcaatcg tttcacaggc agcatctgaa taataagcac
    19381 cacctcgttt ttgcaattgt tcaggtttat gatccaattc tggatctttg tataattcaa
    19441 acagctctgc ttctgtttct ttcacttgct gcgctcttgt accaatcgtt tgatattctt
    19501 ctaaagcatg ttccagcatt tcttcttcac ggtagtaata gcggtggtag ccacaaggaa
    19561 tcatgttcat ttgttttaat tgttctttga aaaacgggat atcaaagata tttttcggca
    19621 atccattgtc ttcatcgtat aaacgatcaa tcaattctgc cgtgcggtca tttcctttat
    19681 tatctgctac tttgtgccaa tggaagtgat ttagaccggc aaacttgtag atcagctctt
    19741 cttttttctt gccaagcatt gttggttctg tcatcatcgc cattactggc acattgcata
    19801 atccgatcac tttatcccat ttgccatatc tgataactgc ttctgtcacc atcccgcttg
    19861 ggttcgcgaa attgatcagc caagcatttg gacataaacg cttcatgtct tcaacgattg
    19921 ccaaaaccac tggaacggta cgaaaagctt tgaatatccc gccagcacca tttgtttctt
    19981 gcccaatcat cccataataa ctagggatcc gctcatcatt gattcgtgca tctaattgac
    20041 cgacacgaaa ctgagtcgta acaaaatccg catctttcaa tgcttcttcc cgatccagag
    20101 ttgcatagat tttgacttca tacggagaag catcccacat acgctgagac atctgactga
    20161 cgatctcaag cttttctttt ccttcttcta catctacgag ccagatttct cgtatcggta
    20221 attcctcata tcttttgata aatccttcca tcaactcggg ggtgtaactg ctgcctccac
    20281 caatcgtagc gattttgatt ccttttgcca tgttctcact cctttacttt ttacactttt
    20341 attatctttt ttgtaaacaa aacaaacaat agaaattcgt ttctttccag taacatttac
    20401 aaaagttagt gttttcttct ggatttttgt aaatatcttg tcaaaaaatg tctttattaa
    20461 gctatataac ataaatccgt ttacaaatca ttttcaaaaa agttataatt tacgtaaaaa
    20521 aacaagaaga aggtatctac atgttattac gtgaaaaaat caaagaaatc tccttttctc
    20581 cttcagaaaa tgaagtgatt cagtttctct taagcgattc tccagcactt ggcgaaatga
    20641 ccatccaaga gattgctgag cgatgttatg tccacccgtc cacactcatc cgtgtagcaa
    20701 aaaaaatggg ttttgatggc tgggtcagtt ttcgtaatgc ttttttagct gaaacagcct
    20761 atcttgaagg gaattttcaa gacgtagatg cgaatcttcc tttttccacc acagacggta
    20821 ttatgacgat cgcaaataaa atggcttctt tagaacagat gacgattcaa gatacacttt
    20881 ctctcattca tcatgaccaa ctgcaaaaag ccaaacaact aatgcttcaa gcaaaaaaaa
    20941 tcgtgatttt cgccagtaac tcgaatactt tgatttctca agatttcatg ctgaaaatga
    21001 agcgcatcaa aatggatgtc acgattgtca ccacttttgg tgaatcgttc tatgaagcgt
    21061 acaattgcac gagtgatatg tgcgggattc tgatttctta tacgggtgaa aacaatgtga
    21121 tccttcgtac cgcagaaata ttgaaacaaa atcatgtgcc aattttagct ttgaccagta
    21181 tcggtgattc aacgctatct tcaatgagta cagccgcttt gcgacttacg acacgcgaac
    21241 gactgtattc gaaaattggt aattttacga tcaatacatc gatttgttat cttctagacg
    21301 tgttatatag ttgtgttttt gcagaaaatt atcaacagaa tttagtccat ctaatttctg
    21361 taggacaaaa atttgataaa cgccctatca gctcagccat catgaaagag cctttcaatt
    21421 ttgaaaataa agaagtggac taaaaagaat aattaagcct agtccattct ttcacctaac
    21481 cgttcaggtc agtgcggtga taagtgcatc gtctggcccg accgtctccc cttgattttc
    21541 caaaatatcc aaataatccg tgtaattggt gacgatcaca ggtgtttcta aactgtatcc
    21601 ttcctcttcg attttcttct tgtcgaagcg gagcaacaga tcgccttgtt tcactctttc
    21661 tccttgctgc accaagcttt taaaatattt tccttctaac tgaacagtat ccaatccgac
    21721 atgaatcagc aattccagac ctgcttcaga aaccattccg attgcatgtt tcgtcgggaa
    21781 cagggtcatg actgttccgt caaaaggggc acgtacttct cctttttcag ggtagatcaa
    21841 gattcctcga cctaatgttc cttgtgcaaa agctgcatcc tttgctgcat tcagtggagc
    21901 gatttgtcct tgtatcggtg tttttacaat ttctttacct gatgttttct gtctatcatc
    21961 tgtttcatct gcttcctcct tccaaaagaa aaaagtcagc gcaaaggata ccaccatcgc
    22021 aatggcgatc ccaatcaata cattaatcac atctgttaaa ttaccatctg gtctaatata
    22081 gttgggaatt gtaaagatgc ctagtccacc ttgattccaa gaagttaaat tcattaaccc
    22141 actatacgcg ccccctaacg caccaccgac acaggtaaaa ataaatattt ttttacgtgg
    22201 caatgtaaac ccataaatag ctggctctgt aatccccaca ataccagaaa caatggctgg
    22261 tattgcaatt gctttgtctt ttggatttct aagcttaaag tagagtgcag taagtgctgc
    22321 agtttgtcca aaagacacta ctgcaacagg tgtcatgata ttttgccagc cattcacaga
    22381 aaattgcatc aatcccattg gtacgattgc ccaatggaga ccaaacatca ttagaatttg
    22441 ccagaagaag cctacgattg ctccataaat aactggcgaa agtgctaaaa gtgtttcaaa
    22501 tccagccatt aaaatatcag ttgcgaatgt gaatgctggt ccaacgagta aaaaacctaa
    22561 gggcattgta ataagaacgg tgaagaatgg aacgaagaaa ttcgcgatca tctcaggtac
    22621 taacttttta attggtttct gaatttggct agctaacaac acaataaaga taatgggaat
    22681 gacagaagaa gcgtagttac gcgcaatcca gggaatgccg aatactgttt gataaatagg
    22741 tgcttcaaaa atagtgccag aaaatatggt cgtcagtggt gcttctgctg caccactcaa
    22801 agcttccagc gacaagctag gattcattaa tgctgcccca agcaacatcc ctacgaattc
    22861 atttacttta aatttacggg cagctgttaa accgataata acaggcataa attggaatac
    22921 agaatcaccg atattatcaa acataacaaa tgtgccagaa ccgcggtcaa atgcgcctaa
    22981 gaagacaagt aatgctgtca gtccacgaat catcccacca gcagctagaa tagcaaggaa
    23041 tggctggaaa cagcccgata ttccatcaat aagcatatca aacggtctca ttttttctgt
    23101 tgaaacagcc tcatcaaaag taaggtttcc tgctcttata acctcttcaa acacgactgg
    23161 cacatgattc ccgatgacaa cttggtactg tcctcccgct cgcattaccg tcacaacacc
    23221 gtccatattt ttcaagatct catcatttgc tttttcttcg ttttttaatt tgaaacgaag
    23281 ccttgtgata caatgtgtca aaccattgat attttccttc ccgccgacat tttctacaat
    23341 tttgctcgct aattcttgat attttcccat ttctattccc tccaatttat tagtggaagg
    23401 tttagccaac tgaatgtcac tacccttatt tcatgcgttc ttgttaattt atcctccttt
    23461 tttatttatt tacaacgatt ctcatgcact aaacgcgcag tatggatcgt taaatacatt
    23521 ttttcgtccg tggacatctg gtagttgaat atctcggcta aaaagtctgc tattttctca
    23581 acgcaaccaa acgtatcctt atattttttt tgaataactt ggtacaattc ctcagaaact
    23641 tctcccactt ctgatttttc tccatctagg acgcgtgtca gaaaaaaacg taggtgtgtg
    23701 acgaatcgaa aaaaatggac tgaatcttca tcaaatgtaa tcttatagta atagcggacg
    23761 atatttagaa tatcctgtat tgttgtagta aacaggtagg tattgtcttg ttggctttga
    23821 atgccttgtg cattgactaa atgcaatgcg ataaatccgg cttcatcttc tgataacttg
    23881 atgtggtatt tatccgagat tttcttcact gttttttccc cgatggcata ctctttagga
    23941 aatagttgtt tgatatcata cagcaataca tttggtacaa atattcccat tttgtgacgt
    24001 tcgatagccg tatacaaatg atccgtcaat gcaatataca ctgaatcatt caactggact
    24061 tgtagagcct cttttgtata ttcaatgatt tcacaagaaa tttctaagta ctcaagagga
    24121 atcgcttgtg ccaattcttg gaactgttct gaaagtccgt catttgtcaa ctgaaatttt
    24181 ttgtcgatgc atgagtcaaa aatcgggtca cccactttct ttttgaatgc caaacctttt
    24241 cccatcacga tcatttcctg atttctttca tctctggtta ttacgacgtt gttattcagt
    24301 actttctcaa tcagcatgtt ttactccttt ccatgatata tagggctgta tccaaaacaa
    24361 aaagaaaaac ccaaaaagcc gtccatggaa ccatgggaga ctcatcaggt ttagcttgca
    24421 tcaggcaatc actatccaag ttgtatcgtt ttctttatat tatcgaaagc aagcgctttt
    24481 gtcaacgctt tcttagtggc tattaggcac ttctgactag ctgtcgaatc ttttttaaat
    24541 ctggtgacta cgacactacc gctcacagat gaagaatctg ggaagctctg ttttattttc
    24601 actctagcgc ttaggtcaaa atgctactat gctactgtcc caacttctaa tatacggtgt
    24661 tccagtatca gcttatcggt gataagccaa atttatgcaa aaatctggaa agcctttttt
    24721 acaatttttt tcttatcacc taaagctgaa cggagccctg tcacaaccac tttttacaca
    24781 ccactgcttt gcagtttgta catatctgca tacaaaccat tttgcgctag aagtgtttca
    24841 tgattcccgc gttctactat atgacctttg tctaaaacta aaatcaattc tgcatcccga
    24901 atcgtggaca gccgatgggc aatcgcaatc gttgtccgtc cttgtcgcat ttttgtcaat
    24961 ccttcttgga tcaatccttc tgtttcagta tcgatatttg ctgtcgcttc atccaatact
    25021 agtattttag gatctgtcac gattgttcgc gcaaaagtga tcagctgtcg ttgtccgcta
    25081 gaatagctgg caccgtgttc gatcactttt gagtgatatc cttttggtaa ttcatcgatg
    25141 aacttgtttg cttgaacaaa ctcagctgca tggcgaatct gttcgtctgt gatagttgga
    25201 ttcatcatcc ggatatttcc tgcgatatct ccataaaaca agaaggcatc ttgcaagacc
    25261 aatcccattt tcttacgtaa ctcttccatt ggatattctc gaatgtcatg atcatcaatc
    25321 aagatctggc cttcataaaa ttcgtaaaac cgcatcaata cgttgatgat cgagcttttt
    25381 ccgcttccgg tgtgccccac taatgcaacg gtctctcccg gattagcgac aaaagagata
    25441 ttgttcaata cattgtgctc tccatcataa gaaaagctta cattgcgaaa ctcgatctta
    25501 cctgaagtaa tcgtttgatc tgctccagga ttttgttgcg gtgcatattt ttctgtatcc
    25561 atgattttca aaatacgact accggcaacc attccatctg taaaggtgct caaaaagtcc
    25621 atcatttgag acattggatt aaaaaatccc tggacatagg tcgtaaatgc atagatcagt
    25681 ccaacttcta cgggagaatg cagcgcatcg atcccaaaca aagtcaacgc caacgcaatc
    25741 gccaatgcat atagaaaatt gatgatcgga cttaacagaa tcgaattcgt tttgatcatc
    25801 gcatagcgcg tgtctaaata ctcgttgttc acttgttcaa attctttagc taaacgagct
    25861 tcttggcgaa actgctggat aatacgcatg ccggaaatcg attcattcaa tttcgtgttc
    25921 agctgactta atttttcccg catattccgg taaatacgag aactgaattt ctggtaatac
    25981 cagatgacga ttcctaaaat cggcaagaac accaaagtcc acaaggcaat ttctccattg
    26041 atttgaaaca tggcaatgaa ggaagaaacg actgcaaaga tcccagtcaa taccatcaaa
    26101 aagacatacc aaaattcaaa tagcgtttct gtgtcatttg tcactcgaga aacggtcgat
    26161 cctgccggtg tctgatcaaa ataacgcata cctagtgtat gcagcttttc aaataatctt
    26221 acccgaacat actgataggt cttcaatgaa gccatggaat acaaatacca ttggaaaaac
    26281 caaatgatac ttttgactat gactccaaac aagtacatac cagcaaaaaa caaaataact
    26341 tgtgtcgtag ccgactgttt agccagatga tcatccataa aagtttggat gatccttggc
    26401 aataggacat tgatcacaga cagagcaaac gcaaataaga tagcaacgat gaacgttctt
    26461 ctaaaaggtt tggcgaactt catcagtcgt ttgatgatct tcttctgttc tttaagtgga
    26521 atcgattttg tccattccga ttgataattt tcatccatca ggcctcgcct ccttctatct
    26581 tcgcttctaa ttgctgtttc tcccacattc gttcatacca gccgcctaat gcaagcagct
    26641 catgatgggt tcctcgttca atgattttcc cttgatcgac aacaatgatt tcttttgcat
    26701 gcatcacgct gcttaaacgg tgagctgcaa tgatggttgt tttctctttt cttatttctt
    26761 ttaaattatg caagatagct tcttctgttt tggcatctac tgcagaaagt gcatcatcta
    26821 agatcagcaa ttcaggattt ttgatcaatg ctcgagcaat cgaaatcctt tgtttctgtc
    26881 cgccagataa agagactcca cgttctccaa ctaaggtatc ataacctttt ggcaattcct
    26941 tgatttcatt gttgataaat gctaaagaag ccgcatgctc tacttcttct tctgaaagtt
    27001 ttggattaga aaaacggata ttatctcgaa tggtcatcga aaacaagaag tgatcttgag
    27061 gcacatagcc gattgatcct aataaagcat tcaatgaata ttctttgatc gaacgtccgc
    27121 caaattggat ttttccttga taatcatcgt attcacgcat cagtaatttg atgatcgtcg
    27181 tttttcccgc accagttttc ccaacaatac ctaatgtttc tccttcttgg atcacaaatt
    27241 tgatttgttc taatgctggt gattcttctt tcggatagtt aaaagaggaa atagctacat
    27301 tcagctcgcc ttttgccggg gtctgcactg cacccagttg ttccacgata tgcgtctttt
    27361 catgcaaaag ttcattgacg cgatcataag aagcatttcc tctttccaat acgttgaaca
    27421 atcgaccaat cgcaaacatc ggccaaacaa gcataccgat atagctaata aatgagacca
    27481 tctgcccgat cgagatcgtg ccttccaaga catactttcc acctaaaata attgttgcaa
    27541 cataagacag accgataatc aaggtaatga atggatcaaa taaggagtct aaaaaattga
    27601 ctcgtttgtt tttaataatt gcatcttcaa tcttttcttc aaaatccgcg agatcttctt
    27661 tttcttgtcc aaatgtttta atgactttga ttccagtaat actttcttgt gtcttatcat
    27721 tgatactaga aaaagcttct tgcgaatcac gaaaagcatc gtggagtttt gaccccagca
    27781 ctcgtgaagt aacggctagt aaaggcagag gaatcagcgc aatcagtgtt aagcgccaat
    27841 caataaacaa aatcatcgca atgatcgttg ttccgcccgt gatcactgaa tctgcaaagg
    27901 tcaagatacc agctcctgct acattttgga ttgcattcaa atcattcgtt gcatgagcca
    27961 tcaaatctcc agtccgatat ttttggtaaa agagattgtc catttctgta aaatgtttga
    28021 atagctgctg ccgaagttct ttctccaacc gagctgcact tccccaaata ttcgtccgcc
    28081 agatataacg aaaaagatat tgtaagagag ctgcaaggat caataataag atccaccagg
    28141 aaatgatctt tattgataaa tttttatcgg caatctcatc aatgatgatc ccaatcacct
    28201 ttggaggaac taactgtacc aaggccactg cgaataggga gaaaatccca attaaataat
    28261 gtttcttttc ctgtcgaaaa aaccaaccta attttctaaa aatcgacatt ttactcctcc
    28321 tttgtcttta tcaagagcat aggattgtct gtgcttgtct tttttgctga atagtctgaa
    28381 tagtctaaca aattacgaaa taaaaaacgg actgaaaaaa tcagcccgca aaaagtcttt
    28441 gggtcagaac ctattttttc ccttggttct tcatttgctg catcatctgg cgaattttct
    28501 tctcagatgg tttttgcccc attgacatca tcattgatcg caacatgtcc tcattaacgg
    28561 gaggattttt ttctaaatag tctttcatat atttacgcgc taggaaaaag ccacctactg
    28621 cgccgattag caaagcaata atagcaataa gtacgacaat acctgttgat accatataaa
    28681 tctctccttt ctatttccga attgctctca atgatttatt ttactaaagt ttattgaaaa
    28741 aagaaaggcg tattctttag tttcatgcaa aaaatagtgg tttttttaga gaagaacaaa
    28801 atttctttca ttaaccagca agacacgcca cgaaaaaaaa ttaggcatac cgaatatttt
    28861 tctttaattt tttctttctg aggtgtagaa tgtaatcgta gataaatagg aaaggtggtt
    28921 ttttcttgaa taatcaatcc aacaaacaat ttcataagca tcgacttatt gaacatacaa
    28981 atggtttatc tttagctgaa gtaaatggaa cagtggatgt acccaaggga aaaagttttt
    29041 ttaaaacact tcttgcttac tctggaccag gtgcattagt agcagtgggt tatatggatc
    29101 caggtaactg gtccacttcg atcactggtg gacaaaattt ccaatattta ttgatgtcag
    29161 ttattttgat gtctagtctg attgctatgc ttttacaata tatggcagca aaattaggaa
    29221 tcgtgaccca aatggatttg gcccaagcaa tccgagcacg gacaagtaag acattgggtg
    29281 tcattttatg gatcttgact gaattagcca ttatggcaac agacatcgca gaagtcattg
    29341 gtgcagcaat cgccctctac ctgctttttg atattccatt agtgattgcc gtatttatca
    29401 ctgtgttaga tgttttcgtc ctcttattgc tgacaaaagt cgggtttcgt aaaatcgaag
    29461 ctttggttgt ctgcttgatt ttcgtcattt tattcgtttt cgtctatcaa gttggcttgt
    29521 ctaaccctga ttggcacagt ttgtttgccg gctttatccc taacagtcat acattctcag
    29581 actcaccggt catcggcggt gaaactccgc tgactggagc tttagggatt attggtgcaa
    29641 cagtcatgcc gcataactta tatcttcatt cagcagtttc tcaaactcgg aagatcgatc
    29701 accaagatga agaagatgta gcacaagcgg tacgcttttc tacttgggat tctaatatcc
    29761 agttgtcaat ggcttttcta gtcaatgcac tgctattgat catgggtgtc gctgtattca
    29821 aaaccggttc tgtccaagat ccttcctttt ttggtctgta tgaagcttta tctgatccat
    29881 cagctatgag caatggtatc ttgattgctg tcgcaaaatc tggtatcttg tccactttat
    29941 ttgctgtagc acttcttgct tctggccaga actctactat tactggtacg ttgaccggtc
    30001 aagtcatcat ggaaggcttc atccatatgc gtatcccgat ttggctaaga agactagtca
    30061 cacgattgct ttctgtgatt cctgtattga tttgtgtttt gtttacaagt acaaaaggaa
    30121 cgatcgaaga acataccgct ttgaataact tgatgaatga atcccaagtt tttctggctt
    30181 ttgcattgcc attctcgatg attcctttac tgatgatgac caatagccaa gcggagatgg
    30241 ggagccgatt caaaaattca tttgttgtaa aactacttgg ctggttctct gtgattagtc
    30301 tgacttactt gaacttacga ggattaccag gtcaaatcga aagtttcttc ggcgagcaag
    30361 ccacctcagc tcaactagca ttggctgatc aaatcgctta tgtaatcatt gttcttgtcc
    30421 ttctcctgtt ggcatggacg attggtgatt tatataaagg aaacaaaaaa tatgaacagc
    30481 gtttgcaaga aacgatagaa gaaatcgaat agaaactgat tcttttacac aaaggaggtt
    30541 gtgacagaag tgggcagctt caagaaataa gaaggaattt aagaaaattg cttttcaaat
    30601 ttttgtgaaa tttcggctta tttttcgaag aagttacttc tggaacaccg tgtatcagag
    30661 gttgtgaaaa agctgtcctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
    30721 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
    30781 gaaaaaggaa ctgcgacaag acttttgtca cagttccctt tttatttaga agataaagct
    30841 tttttcagat tcaatttact gaaaactagc atgcttgtta ctacgatgaa ttgtgttaat
    30901 ggcacgcctg cgccagtgta gacacttaaa aaataatcct ttttttctgg cgcataagaa
    30961 caaaaccacg agacaccgaa aagaaaaacc aattgtgtca gtattaatcc ggtgactaca
    31021 atcaaagagg aactataata ggcacgatca gcttttgcaa atacggttcc tagtatcata
    31081 agtaatatcc ctgctattga agcaatcagc acgggaatgg ccagaacttg gtaaattttt
    31141 atcatgacat tcgcgacttt tgctgttttt gtcaactgga aatcaggtgt tctcggatca
    31201 ctgctatcga tccaattatc cattagtgga agattcaata attcttgggc ctgttcgact
    31261 tgttcttgcg tcccgaaact ataattccct ccttgatgga aatctttgta tcgccatgca
    31321 taggcatatc ctgatttcag aaaagcaaat acgattggca gatcattcat cgtctttcca
    31381 tcccctgatc ccatcagata gaattctttt ttctttttga tagttccctt ttgataggct
    31441 tcttctaatt cttgattgac ttttttccaa aaatcattgg tttcttttgc attgttttgg
    31501 taataaccag attcgcttac cacatctcgc aatgcccaaa aaatgatatc tcctggtaat
    31561 tcttttactt ttccgccttg ggtccacgca tgagtcgtat acaattcgtc aatacgagta
    31621 gaaacagtat gaaatgtagg agatgcgtct aatgctttat ttaatgcagc atgcgagacc
    31681 catatctcac tgttttcttt atttaagttc gtttcgtcat ctattcggat cagctgtttc
    31741 atcaaacgtc caaattcacc actcgaacgg tcattgatgg tggcaatacc atatgttgtc
    31801 tcgtttttat attcgatcca ctgatagacg atggagaaac cgactagagg gagtaaaaac
    31861 aatgaaagtt tcagccattc ttgtttcgaa aggagaaatg gtcgttcttt taacttcttt
    31921 gcttctttga aagaaaagtc ttgtcctagc aagatctgaa cgaaactgat gcctaacgcg
    31981 actacagtaa aaggaagaag ccagatggaa tcttcgcgaa tgaaccaaaa gaaaggaaaa
    32041 gaaacagcta accctaaaaa ccacggcagc aattttctgc gggaactata tttacggtaa
    32101 taaatagcta gtaaacaagc gataatgatc tcaacagccg ggatcacgag agcattacga
    32161 tacgttcgag tggaatactc atgtgtaaaa ctaatcggag aaaacaaaaa ataaaaatag
    32221 atacttaaaa gaagatactt gttcttcacg atcggtttaa acgctagtgc aaccaatatg
    32281 ctggcaagaa tactgaacat gccgaacaaa actgcataag aaagatgcag tttatgtgca
    32341 agtactaaaa acaagctgta ggagatccct tttgaaagtg tcgtagtcgt gtactcccct
    32401 agccatttac catccaaaat attttgagca tagtgaatca gcagctgatc atcataggtt
    32461 gccgacatat ctgttccata gattgcttta ctatataaat acaaccgaat aattgtaaat
    32521 aatacggcta tgccaagaat aaagtatgta tgtttctttt ttagttggtt gatcaagggt
    32581 tttaaagtgt tcagcatctt ttcgtccttt caatcacgtt ttgctacttg cttgtgaatc
    32641 ttttcgtttt ttacttttct atcagtatac cctgtttgtc tgaatttaaa aagagggggc
    32701 gaaaggagca tggcgaaaac gactgcaaaa agcactcctt tgtttcttaa acaaaaaagg
    32761 acaaggcaga ctgcccaatc cttttttgtt gctgtcagat agaaaactta aagtgtgact
    32821 tcttctgcta ataactcagg aaacacttcg ggatcgaatg cttgcgcttt gaacatggca
    32881 atttcttttc catatggtgc gtaagctttc tttttatctt taccgacata tggagtttct
    32941 aagattttcg aaatgttgtc aaacattgga tcatggacga ttttgttcaa tgtgtcaaat
    33001 ccaattgttc caaaaccgat attggcatgc cgatctttat gagaaccttg tggattttta
    33061 gaatcgttga tatggagtac tttaagccga tctaatccga tgaccttatc aaactcttcc
    33121 atcacacctg agaaatcttc ttttatatta tatccggcat cattcgtatg acatgtatca
    33181 aaagtaacgg ataatttttc attcaatttt actccatcga tgatttgcgc tagttcctca
    33241 aatgttcgac cgatttccgt acctttccct gccatcgtct ccaaagcgat ctgtgccgtc
    33301 tgttcttttg tcaaaacttc attcaaaccc tcaataattc tagcaatccc accttctgcc
    33361 cctgctccta catgagcgcc tgggtgaagg gtgatttgag tagcacctaa agcttccgca
    33421 cgaacaatct cttctctcag aaattgtaca gcaaatggaa acatttctgg tttgatcgta
    33481 tttcccaaat taatgatata gggcgcatga acgacgatat ttgttagtcc gttttctgcc
    33541 ataaatgctt ttccggcatc aatattcatc tcttcgatag cttttcttct agtattttga
    33601 ggagcgcctg tatagatcat aaaggtcgaa gatccgtaac tggctgcttc ttctgccgcg
    33661 cctaacagca ttttctttcc gctcatgctg acgtgtgagc ctaataacat ggatggattc
    33721 ctccttgaaa ggcaattgtt tcgaacaatt cctatatatt ggtaagatga tgtacaaaga
    33781 aaatggtttg tttttctttg ccctgttctc catagtaact gaaactttct ctaaagaaaa
    33841 gcttaagaac tctttcataa aaaagatcat ttcctcacgt tttatctcat tcgaaaaaga
    33901 aattcctgtc aaaaacagcc ttgtctgttt ttttgtctta ttatttgcta taattagcga
    33961 aataaaacca ttagttgagg tgaccctttt gccacgcaaa aatactcgaa aaaaacagaa
    34021 tagaagaaaa aaagaaaaat ggtttgttcc taaggttatt tttagagtgt ttcaatcatt
    34081 gactgttttt attactgttc ttcttttgtt gttcgcagct ctaggtatcg gaataggtgc
    34141 tggctacttt gcctatcttg tcgaagacac acaattgccg actaaaaaag aactgcagac
    34201 agaactagga aacattacgg aaacgtcaaa gattgtttat gcagataata cagagatatc
    34261 aaaaatccag acagatctga tgcggacgac tatttcatct gataagattt cgcctttatt
    34321 gaaaaccgcg attatcagta cagaagatga atacttcgat aaacaccaag gttatgtccc
    34381 taaagctgtt ttacgagcat tagtttcaga agctactggc atcggctcgt ctggaggatc
    34441 cactttgaca cagcaattag tcaaacagca gatcctaacc gatgaaacaa cattcaaacg
    34501 taaagcaaac gagatcgttc tttctgcaca agtagaaaaa tacttttcaa aagatgagat
    34561 cattgctacg tatttgaatg tctcgccatt tggtcggaat aataaggggc aaaatattgc
    34621 tggagtccag gaagctgcaa gaggaatctt tggtgtagat gcaaaagatg tgactctgcc
    34681 acaagctgct tatatcgctg gtttgcctca aagccctatc acgtatagtc cctacacgaa
    34741 tacaggcgcg ctaaaagaag atctttctgc cggtcttgcc agaaaagatt ttgttttatt
    34801 cagtatgtac cgtgaaggac aaattacgaa agaacaatac gaagaagcga aagcctatga
    34861 tcttacaaaa gactttctcc cacaacaaat cgcggaacag actgacagag aattcttgta
    34921 ttataccgtg atgaacgaag caacaaggat tattgcacaa caattggcag aaaaagataa
    34981 tgctgatatg tcagattcat cggtcagtga agcttattat caaaaagcac agcaaacgat
    35041 ccaaaacaaa ggatatacga tccattcgac gatcgataaa gatatctatg ctgcattgca
    35101 aaccggcgta gaaaattacg gctatatgtt agatgatggt gctggtagcc aagtggagac
    35161 tggaaatgta ttgatggaca ataggactgg acgtatctat ggattcgtgg gtggccggaa
    35221 ttacagccag aatcaaaata accacgcctt tgatacagaa cgtcaagctg gttcatccat
    35281 caaaccggtt cttgtttacg gtcctgcaat cgatatggga cttgtcggat cagaatcccg
    35341 tgtatcagat tatgcaacga cttggcaaga aggagaaaat gctggcgaga aaatcgtcaa
    35401 tgcgaccaat gaagggtcga acaccttcca aaccgtccgt gagtcactgg aatggtccaa
    35461 caatatccca gcttatcact tataccaaga cgtattgaat tcgggtggat cgaaacaata
    35521 tgcttacgag cactacttgg cgaagatgaa ctacccagca aatgataatt ggggcgttga
    35581 atctgctcct ttagggacag tagatgtaac gacgcttcaa cagacaaatg gattccaggc
    35641 cttagctaat ggtggcgtct acgaagaagg ctatatcatt gattccatca cagataatgc
    35701 aggtaatgtg atttataaac atgaaagtaa ttctgtccgt atttattccg aagcaacggc
    35761 ctcgatcatg aatgacatga tgcgcagtgt gatcaatgaa aaaatcacta caccattcaa
    35821 agatgtcatt tcaagcttaa acggaaatct aggaaaagca gattgggtcg gaaaaactgg
    35881 ttctaccaac gaatacagag attcatggct gatcgtctct actccgtcta tcactatcag
    35941 tagctgggca ggacacgatg acaatactgg aatggacagc caagcaagga tacgttcggc
    36001 taattatctt gccaatctaa tcaatcaagc ttaccaagca aaccctgaga ttttcggcac
    36061 tgatgagaaa tttgacttat catctgatgt gatgaagaaa aaagtatctg ggttcacagg
    36121 acaaacagct ggaaaagtta ctgtcaataa aaaatctatc caaacgccag gaaaaacagt
    36181 ggaatcactt tgggcaaaaa atggtccgaa gaaaagcgcc tttaaattcg gcgttggcgg
    36241 tactgatgaa aactatacag attactggaa caccgcttca gcttatgcta gagcgaatcc
    36301 agagaccaaa gaatcagacg ataaagatga ttgatctaat agattaatca gaattaaagg
    36361 gagctgcgac aaaaatcttg tcacagctcc ctttttccga ataaatggtg ttcaaaagct
    36421 tgcatctacg atattaagac aaatttatgt aaaaatgtga aaagccattt tcacaaaatt
    36481 tttctatata actttactaa cgaaactgtt ttaatttctt ttcttgtctt tggaataatt
    36541 cggttagttt ttctgcaaat tgccctttta ctgattcttt caagtgtaca tgtcggttag
    36601 aaaattcttg agcagcttct tctgcagtca tttggtggtt ttgatctgtc aatcgttttt
    36661 ctgcccaaat ttgctgagat atccccatac caactgaacg tacatttgat cgatgtagat
    36721 ttttctctaa ttcttttagt aattgcatta aaaattcatt cataagatga tttctccata
    36781 gttatttgtc tgattttttg acgataggaa cgttctagtt cgtcttgctg ctgatagagt
    36841 tttcgtcgtt ctacttgtag ggcttgttga taatgctcct gataattcga ataacttcgt
    36901 cttgcttgaa acagaggctc attatctaaa gctaagttct ttaatacatg atgcagtcgc
    36961 tgctcctctt gctccatcaa tcgtaatcct tgattcttaa agtggatcaa ttcatcttct
    37021 tgagcttcta attgtcttcg ttcacttcga taatctcttt ccaattcttc tagtttcatc
    37081 tacttctcct ttaattgaat tgctgtgcta attctcgatc tcgtgtcacg atttcattga
    37141 ttttgtcttg gatctccttc accaatgtat caaaggcctc ccccatacgt cttgccttat
    37201 tgattcgcag cagatagact tctttcgcag aatcgactaa gttctctttt gtggcgacaa
    37261 cactagctaa tgcttctatg atttcctgtt cagtcaacga aggggcgaca acacgtgctt
    37321 gccgcaagcc ttcttgccat gccatttttt ctctagctca aaaattcctc tttgattgat
    37381 gcggataact tcttccatag cgcttttcat ttttgctgat aacttttcca ctactaacct
    37441 agcttgtgtg tcatctaagt aaatttgttc attaactgtc agccggccgc cacttaatcg
    37501 tagtttttta cgtatgttgt tcagttcttg caactgatag gaaaacaact gggtcgcttg
    37561 gttttctttc tgttgttttc gagctttaac attattttca ttttcaggaa taatcagctt
    37621 tccattttca tcaaacttcc aagaggttaa actgtgggaa gcaagcagat tcaagaatgc
    37681 tgtttccata ctgatccgaa cttcagcacc tgtgccatta ccaccaaaat taccgataaa
    37741 atcggcgtga tttcggtaat tggtgagcat accgggattt tcttccaccc atttttttgc
    37801 ttctggtgat aagagatcat acgggtccgg cccgtttaag ccgacatttt tccattgttt
    37861 ctctgctgct acatataatg ctaagaatcc acctaatgaa tggccggtag tagaaatagc
    37921 agcttctcca tattcctttt ttacttcatt agcaaatgca atcgctgaat tgatttgccc
    37981 cgctgttttc aatccactaa cggataaaat cttgtttgtt gttgcttttt cagaaaaact
    38041 tgctcctggt ctattcccat agagattcat gatactcctt gcatccgttt ccaaatcttt
    38101 ccaatcactt aagttcgttc ctgcaaaagc aatcactact tgagaaagat ccactttacc
    38161 gttcttgatt ggcgctacac ccatcgcctg catgccgtcc aatgagtctt tgatttttag
    38221 gattttaaaa ttataactat caattttttt aatagaattt tctttataag tatgatcata
    38281 gtctctgtgt ttaggatcta gccagtaaac agcctcacta ataacactgt attgtttgtc
    38341 tgccatcatt aaatatattc ctccttatta ttagaaaaaa ttactctgat tttatttact
    38401 gtaatcccct ctttttgtat acttttattt tctacgccat aatctcctaa ttcatttcgt
    38461 tctttccaaa atccaaacgt aaaataaaca gactcatcgt gattatttat cattgtcaca
    38521 aacatacgat atgctcccgt catttcattt cgagcagatt tttcaatctt cacttccttg
    38581 atatccgcaa aagtattttt caaaactttg actgactgtt tttctactgc gagtaaatct
    38641 ttctgtcttt gtgcttctct tttgtccata tagactttcc ctccgattcc taatatgacg
    38701 attaataaaa tggctaagct gagttttctt attttccgag acaagaacgt gcctcctttc
    38761 tttttatgta agtattgata ccttcattat ttaagtacgc gaaagtttgt aggaattttt
    38821 ttatttttga aatttttctc caatttgcta tggacgattg gcgctgcagc catcgcctgc
    38881 atgccatcca atgagtcttt gatttttagg atttggaatt ttgtttctcc aaagttttta
    38941 atggcgcctt ctttgtaact tttactatat tgaggatgtt ttggatctag ccaataaaca
    39001 tgttcactta tatatctata ctcttcgtct tttactgtca cacttcctcc gcctctccat
    39061 tagaataaat aactcttatt ttaggagtag taaccccttc gttttgtatt tcttcatttt
    39121 ctacaccata actgctaatt tcattcattt gttttgaaaa aaaataatca aaataaacag
    39181 atttcccttc attatttgtc attgtcacaa gtgcaccata acatccagta acagggtttt
    39241 ttttaaattc ttctaccttc acttccctga tatctgcaaa ggtatttttc agaactttga
    39301 cggattgttt ttctactgcg agtaaatctt tctgtctttg tgcttctctt ttgtccatgt
    39361 aaaattttcc tccgattcct atagcaagaa tggtcaaaat gatgactatg gtctttcgtc
    39421 ttttcttagg caaatagaag tctcctttcg ggttatgtat gatggattgt ttctccttta
    39481 agataagtac gcaatcgagc ctggattttt ttggattttt tagagttact tttctacttt
    39541 tctactttct tttttatcac gttttcattt taaagtaatt ttgatagtag tgaacaaaat
    39601 gtagcatcta cgcacagatt gtgtcgtata cgatggacgt aaggtttgtt tccgccattt
    39661 tctctttttt taaagtcaca aaaaaaaggg gttatgacaa aattttgtca taaccccttt
    39721 tctcgaataa acgttgttcc agaagttggc cacttttgga acaacctcta aatgattgat
    39781 cttttattat ttagttgatc ctttttcttc gattccataa ggcaagataa tcgttttttc
    39841 ttcgacttct tctttattca tcattttcgt caataggcgc atggataccg ctcctaaatc
    39901 gtataatggt tgtgtgatac ttgtcaaacg aggacgagct acttctgtca gtaaagaatt
    39961 gttgcttgtg atgatttcga attcttctgg tacttttacg ccacggtcta ataaaccatc
    40021 caagatacca atggctaatt catcgtctga aacatagaca gctgttgcac cgctattatg
    40081 gatccggtca actagagaca gaccatcttt aaagttgtat tgtgcctcaa agattaatcc
    40141 ttcgctatat ggtagattgt tttcgcttaa tgcttcttta tagcctttta gacggttttg
    40201 accattgata ggatcaatca gcgcaccgct gacaaaagca atttttttat tgccgctttt
    40261 tgctaattta tttactgcat cttttgttgc tgatacataa tcaatgttta cgctacctac
    40321 ttgttcatct ggatcaatgg aaccagctag tacaacaggt gtttttgaac gtgaaaattc
    40381 tccacggatt tcgtctgtga tatggtgacc cataaagatc acgccgtcca cttgcttagc
    40441 caataaatta ttcaatacat tgacttcttt ttgatcgttg ccatctgagt ttgctaagat
    40501 gatattgtat ttgtacattg ttgctacatc gtcgatacca cgtgctaaag aggcaaagaa
    40561 catattgctg acatcaggaa tgattacccc aacagttgtt gttttcttac ttgccaagcc
    40621 acgtgcgact gcatttggac gataatccaa gcgatcgata acttccaaga ctttttttct
    40681 tgttgctggt ttgacgtttg gattgccatt tacgacacgt gaaacagtag ccatcgaaac
    40741 atttgcctct ctcgctacat catagatagt gattgtttgc ttttccattt gtgttctcct
    40801 attcatattt tatatgtttt ctgaaaatac gtcttacatt tctaaaatag gatatcaaat
    40861 aatctcaaca attgcaaccg ttttatctct gttttcatga aaatttttac ttttttcagt
    40921 tttaagaacg ttggtctttt aatcaatcag tggtacaatt attgaataca aaattaattt
    40981 agaaagtagg ttgccaaatg aataacgtaa aaatcaatga actacgtcaa tggatggcac
    41041 aacaccaagt agaattggct tatatcagcg accctggcca catcgcttac ttttccggat
    41101 atgaaagtga tcctcacgag cgtgtactag ctctttttat ccctcttgaa caagatcctt
    41161 tccttttcac tcctgctctg gaagttgaag atgctcaaaa cagcagctgg gcgtacgatg
    41221 tacgtggcta cttagacagc gaaaaccctt gggagatcat cggaaaagag cttcgcgcac
    41281 gctatgggca acctggtaat gtagggattg aaaagaatgc tctgactgtc gatcgcttcg
    41341 atgctatctc tgatatcttg acaggtgcag attcttacac tgatctaact tctattatcc
    41401 agcgtctgca actgatcaag acggtagaag aaaaagacaa actgatcgaa gctggtaaat
    41461 gggccgatgt tgcctttgaa attggcttca aaacagtaaa agaaggcgtg actgaacaag
    41521 caattatcgc agaaatcgaa taccaattaa aaaaacaagg tgtttcacaa atgtctttcg
    41581 acaccttagt ccttgcagga gcaaatgctg caagccctca tggtacacca ggaagcacga
    41641 aggtttcacc aaatgaacta gtgttattcg atcttggcgt tgtttggaat ggctattgca
    41701 gtgatgctac tcgtacagtt gcttatcaaa aaccaacaga attccaagaa aaaatctaca
    41761 acatcactct tgaagcacag cttgccgcac aagaggcagt tcgcccaggt gtgacagcag
    41821 gtgaacttga tcaaatcgca cgaaacgtaa tcaacagcta tggctacggc gaatatttca
    41881 atcaccgttt aggtcacgga attgggacaa ccgtccacga attcccttct cttgtagaag
    41941 gaaacgactt agtgatcgaa gaagggatgt gcttctctct tgaaccagga atctatattc
    42001 ctgaaaaagt aggggtacgt atcgaagatt gtgtgtatgt gacaagtgat ggttgtgtgc
    42061 cattcacgac tacaccaaaa gaattacttg ttttagagta atgaaaataa aaaaggtggg
    42121 agctggtccg ctctcacctt tttattctag aaatgagtat tcttagtgat tggtagtcga
    42181 atctgtcatt gtatctttca tttctttttt agcttctttt gcatcttctt ttgccatttt
    42241 caacatttct tttgtgtcat ctgcaactgg ttttgcttct gacattcctt cattgaccgt
    42301 ctctcctgct ttatctgcag catcttttac atcgatcatg atatcttctg aagtatcttt
    42361 agcaatacta cctaattcgt ctgctggttt tttcagatct tctgctgttt ttttgaaacg
    42421 attagataaa tcagctgatt gttctttgag gtttaacaag atatcatctg attcacttcc
    42481 tgcttcctct gctgattcac ttaccttttg cttaacatta cctgctagat cccctgcttg
    42541 ttgagaaaga tcttgcgctt tttcttttgc aatgcctgtt aactcagctg atttttcttt
    42601 tgccatatct gtgtaatctg aagccatgtc catcaaatca tctgcttgag cagccaattc
    42661 atcccgaagt tctttaccag atttgggtgc taacaataat gcggcgattg ctgcggctgt
    42721 acctccaact actgcaccta ataaaaatcc acctttttta gccattctta ttttcctcct
    42781 gtcgtttcag tatgtttttt gtttttaaaa aatttcatcg ctgttccacc gacttttccg
    42841 gcaacagtcg ctttagctgt cgtttttccg attccgccga ctttcgtcac gatgttttta
    42901 ctagaatgat taagttccga tacactttca cttaaatcag caactgctgt gaacaatgga
    42961 tcgatcgttg cgacttttcc attgatatcc aagagcagct cattactttt gaccagcaag
    43021 ccttctactt gtctagataa aagatctacg tcttttgtga cgatctcaat tgtcgtattt
    43081 gcttctgcga cagtctggtc tactttttct aaagttttgg aaacttgcaa caatacgcga
    43141 atgatgaaaa cgacaagtat cgcaaaggcg attgctgcga tcaatcctgc aatttctcct
    43201 cctgacatga tcaaactccc ttcttgtctc tcttttctat aaggatagca ttttcgtgtt
    43261 ttttaaacaa ataaaaacga gcttcaacta ctttttattc aattagcagc tgaagctcgt
    43321 tagaaaaaaa tgattatttt ttcttttctt ctttgatttc tgttattcct tttggattat
    43381 gatcgatctt gcttccttct gcttctaatg ttttacctaa agacatgatg tattcacgca
    43441 aaggtgcccc tacttctgga tgtgtcagac catattcaat acttgttttc ataaaaccaa
    43501 atttgtcacc aacgtcataa cgtttacctg tgaatttacg ggcaaatacg cgttgtgttt
    43561 tattcaacgt atcgattgcg tcagttagtt ggatctcatt tccagcacct ggttgttgtt
    43621 ctgataatac atcaaagatt tctggagtca ataagtaacg cccaataata gctaaatcac
    43681 ttggtgcttt gcttggatct ggtttttcaa caaagttttt gacattgtat aacccatctt
    43741 ccagtacttc ccctgggttg ataattccgt attttgaagt ttcatcgtga ggaacttgca
    43801 taacggcaat cgtagaagcg tgtgtacgtt catagtcatc gatcaattgc tgtgtcaatg
    43861 gtacttcgtc ttccatgata tcatcgccta gcataacgac aaacggttca tttccaataa
    43921 aggctcttgc ttgaagaacg gcgtgtccta acccctttgg atgagattga cggatgaaat
    43981 gcaagttgac atctgtcgtt tcttctacta atttcaataa ttcgattttt cctttttcac
    44041 ttaggtttgt ttctaattcg atatttgaat caaagtgatc ttcgattggt cgtttagctt
    44101 ttcctgtaac gatcaagatg tcttcgattc ctgatttcaa cgcttcttca acgatgaatt
    44161 ggatcgttgg tttgtccacg attggcaaca tttcctttgc catcgccttt gttgctggta
    44221 aaaatcttgt tcctaatcct gctgctggaa taactgcttt ttttactttc atatacattc
    44281 gctccttcaa aaaattgtta atgagaattc attttctttt ttcccatcac gtaacatgat
    44341 ttcttttgcg gctgttttga tttctttttg ttcatacagc acttcataga ttgtttgagt
    44401 gatgggcatt tcgatttcca tcatttgggc tagttccatc gctgcttgag tagtattcac
    44461 accttcaacg accattccca tattggctag cacctcttct agaggctgtc cttgtcctaa
    44521 caagttgcct gcgcgccagt ttctggaatg aacacttgtg caagtaacga ctagatcgcc
    44581 tactccgctt aagccaataa aggtcagcgg gttggcaccc attgctacac ctaaacggct
    44641 gatctcagct aatccgcgcg tcatgattgc cgcttttgca ttatcgccaa accctaagcc
    44701 gtgaatagca ccagctccaa tggcaataat atttttcaaa gctgccccca tctctacacc
    44761 aaccacatca gggttagtat agattcgaaa ataatggttc atgaacagct cttgtacata
    44821 ttttgctgct tttgcatctg tgcttgccgc tgtgatcgtc gtaatgtcat gctttgctac
    44881 ttcttccgca tggcttggcc cagaaagaac agcaacagct tttctttttt ctgtcgggat
    44941 ttcctctaac aagatttcag agattctttt atgcgttccc tgttctaatc ccttacttgc
    45001 gtggatgatc actggcttcg tattcaattt ggcaactagt tcttttgcaa cagaacgtat
    45061 cgctttcgtc gggataacga atagtaccgc atctgcatct tttactgcat cttccatatc
    45121 ggtgaatgct cggatggaat cgggtagatg atgatctggt aaataacgtt gattggtgtg
    45181 ttgggtgttg atttcttcta cttgtgaagc acgatgtccc caaatccgaa cgtcatgtcc
    45241 gttttcagcg aggacttgtg ctaaagcagt cccccaagag cctggtccta aaaccgctac
    45301 tctataattc attttaccac tcctgaaatt tttttcattt ttaacgctct aaaaatcaaa
    45361 taatttgttg attttttcct aatgagcggt cataatccgg taaatttcct ttttttctac
    45421 gccaaataaa cagaatcaat gtgccgacaa gtaaaacagc agacaataat tgagaaatgc
    45481 ggatcacacc taaaaagtaa aggctgtctg tgcgtaatcc ttcgataaag aaacgtccga
    45541 aactatacca cattagataa cataaaagga cttctccttt tttcaaaaga tgcggtttct
    45601 tccgcaaaac caaaagcaga ataaatccaa tcaaactcca aagtgattca taaagaaagg
    45661 ttggttgtcg atagatccct tcgatataca tgttatcaat gatgaattgc ggtaaatgca
    45721 gactttctaa gaaacttcgt gtcgtgtctg gaccatacgc ttcgtggttc atgaaatttc
    45781 cccatcgtcc gattgcctgc gccagtaata cacttggtgc agcgatatct aaaaaggtcc
    45841 acaaagaaat aaaatgatat ttggtaaaaa agtacagtgt gattgcacct gcaatcaatc
    45901 ccccgtaaat cgctaatcct cctgaacgga tattgaatat ttggattgga tcagctatat
    45961 attggggaag atcgaataaa acataataca aacgggcacc aataatggaa atcggcaatc
    46021 cccacaacat aaaatctgtc acgtcatctg cttttaatcc tacccgaaca gcttctctgc
    46081 tgctgagcca catggcaata atgattcctg atacgataat caatgcatac cagtagatag
    46141 gaattcctac aagctcaaaa gcaactcgat tgatttgtgc taacatattg tcactccttg
    46201 ctagtcattg cctgagtttt cttcaatcaa gcgacttaat ctttcttcaa atgttttcgt
    46261 tgcgtcaaag cccattgtac gcgcgcggaa attcatcgcg gctacttcaa tgatgattgc
    46321 aacgtttcgt cctgttttga cagggatttt tatttgtgga atgcccactt ccgcgatttc
    46381 aacggtcgtg tcttcagaac ccaaacggtc gtatttctta tctttcgccc atgcttctag
    46441 ataaacagct aactggactt gcatagagcc tcgtaccgca ctcgcaccga aaagattcat
    46501 tacatctatg atcccgatcc ctcgaatctc aatcagatgt tccaggattt taggaggttc
    46561 tccgattacc gtcagttcat cttgctgata gacatctact cgatcgtctg cgatcaatcg
    46621 atgtccgcgc ttaatcagtt ccaacgccgt ttcacttttc cctatcccac tgtccccttg
    46681 aattagtaca cctaatccgt agacatcgac taacacaccg tggacacttg ttcgaggggc
    46741 taagcgccca tctaaataac tagataattc acctagcagc cgagacgttg agataggcga
    46801 acgcagcaca gctatcccat tttcattggc tgcttggatc atttcttccg gagccggcaa
    46861 tcctcttgaa ataataaatg cgggcatatc ttcactacat aatcttctca tgatgatcaa
    46921 acgctcttct gggatcatcc gttccgcaaa actgatttct ttactgccaa atagctgtaa
    46981 acgattatga gaataataat taaaataacc ggtcaactct agtcctgggc gtgagatgtc
    47041 cccagtcacg atgacgcggt ctaaactttt ctcgtcgcct gtgacgattt ccaaagataa
    47101 tttttttact aattggctaa cttttacaac ttctgacatt catgctacct catttctatt
    47161 agagtgttct ctaccatttt atcattgtct agccaaaagt actaccaatt aaaaaaaacg
    47221 tttttattta attaccaatg taatagaaaa cgcattctta attatatatg atttgtcaaa
    47281 ttaagctaga ataaaatgct tcttgtacat agctcaaaaa tatttcgatt ggtgttctgt
    47341 aattcaatga ttttcttgga atataattac gttgattgct gacactggaa ataaatgtct
    47401 gattcacttc tctaaaatc
//
LOCUS       DASHRQ010000017        42851 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.017, whole genome shotgun sequence.
ACCESSION   DASHRQ010000017 DASHRQ010000000
VERSION     DASHRQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.017"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(97..372)
                     /locus_tag="U2M88_001046"
     CDS             complement(97..372)
                     /locus_tag="U2M88_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8373441.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            604..1338
                     /locus_tag="U2M88_001047"
     CDS             604..1338
                     /locus_tag="U2M88_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8373442.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            1377..2519
                     /locus_tag="U2M88_001048"
     CDS             1377..2519
                     /locus_tag="U2M88_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEM8373443.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(3147..3632)
                     /locus_tag="U2M88_001049"
     CDS             complement(3147..3632)
                     /locus_tag="U2M88_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEM8373444.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(3634..5070)
                     /locus_tag="U2M88_001050"
     CDS             complement(3634..5070)
                     /locus_tag="U2M88_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEM8373445.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPH
                     YCIMRKILKENFVDSEDFFDQERYNYELTKRRLQINDLSRFSINVVEVDDYSEITDIL
                     KSIRKKYLRKTIFISGSAVDYTPFTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGN
                     SIVSGVLKQRRNLRKNNIQDVLSLRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLE
                     SGEIVTADGMIEEFELSVQNENIVVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQ
                     ALEKLDTEGEVDKKVEQIVSLINSIQED"
     gene            complement(5103..5954)
                     /locus_tag="U2M88_001051"
     CDS             complement(5103..5954)
                     /locus_tag="U2M88_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEM8373446.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            complement(6110..6355)
                     /locus_tag="U2M88_001052"
     CDS             complement(6110..6355)
                     /locus_tag="U2M88_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8373447.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            6460..7233
                     /locus_tag="U2M88_001053"
     CDS             6460..7233
                     /locus_tag="U2M88_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373448.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLNVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            complement(7286..7600)
                     /locus_tag="U2M88_001054"
     CDS             complement(7286..7600)
                     /locus_tag="U2M88_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373449.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(9119..9337)
                     /locus_tag="U2M88_001055"
     CDS             complement(9119..9337)
                     /locus_tag="U2M88_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373450.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            complement(9352..11979)
                     /locus_tag="U2M88_001056"
     CDS             complement(9352..11979)
                     /locus_tag="U2M88_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373451.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            complement(11966..12184)
                     /locus_tag="U2M88_001057"
     CDS             complement(11966..12184)
                     /locus_tag="U2M88_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373452.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            complement(12181..13056)
                     /locus_tag="U2M88_001058"
     CDS             complement(12181..13056)
                     /locus_tag="U2M88_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373453.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            complement(13321..13620)
                     /locus_tag="U2M88_001059"
     CDS             complement(13321..13620)
                     /locus_tag="U2M88_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8373454.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            13822..14619
                     /locus_tag="U2M88_001060"
     CDS             13822..14619
                     /locus_tag="U2M88_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8373455.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
     gene            14670..15812
                     /locus_tag="U2M88_001061"
     CDS             14670..15812
                     /locus_tag="U2M88_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM8373456.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(15910..17301)
                     /gene="sufB"
                     /locus_tag="U2M88_001062"
     CDS             complement(15910..17301)
                     /gene="sufB"
                     /locus_tag="U2M88_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEM8373457.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(17306..17776)
                     /locus_tag="U2M88_001063"
     CDS             complement(17306..17776)
                     /locus_tag="U2M88_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEM8373458.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(17763..18998)
                     /locus_tag="U2M88_001064"
     CDS             complement(17763..18998)
                     /locus_tag="U2M88_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEM8373459.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(18995..20281)
                     /gene="sufD"
                     /locus_tag="U2M88_001065"
     CDS             complement(18995..20281)
                     /gene="sufD"
                     /locus_tag="U2M88_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEM8373460.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(20294..21067)
                     /gene="sufC"
                     /locus_tag="U2M88_001066"
     CDS             complement(20294..21067)
                     /gene="sufC"
                     /locus_tag="U2M88_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEM8373461.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(21264..22112)
                     /locus_tag="U2M88_001067"
     CDS             complement(21264..22112)
                     /locus_tag="U2M88_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEM8373462.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(22136..22822)
                     /locus_tag="U2M88_001068"
     CDS             complement(22136..22822)
                     /locus_tag="U2M88_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8373463.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(22815..23849)
                     /locus_tag="U2M88_001069"
     CDS             complement(22815..23849)
                     /locus_tag="U2M88_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8373464.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(23912..24168)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(24244..24582)
                     /locus_tag="U2M88_001070"
     CDS             complement(24244..24582)
                     /locus_tag="U2M88_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEM8373465.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(24584..24940)
                     /locus_tag="U2M88_001071"
     CDS             complement(24584..24940)
                     /locus_tag="U2M88_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEM8373466.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(24946..26133)
                     /locus_tag="U2M88_001072"
     CDS             complement(24946..26133)
                     /locus_tag="U2M88_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEM8373467.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(26324..27169)
                     /locus_tag="U2M88_001073"
     CDS             complement(26324..27169)
                     /locus_tag="U2M88_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEM8373468.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            27403..28368
                     /locus_tag="U2M88_001074"
     CDS             27403..28368
                     /locus_tag="U2M88_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEM8373469.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            28745..29788
                     /locus_tag="U2M88_001075"
     CDS             28745..29788
                     /locus_tag="U2M88_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEM8373470.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(29850..30314)
                     /locus_tag="U2M88_001076"
     CDS             complement(29850..30314)
                     /locus_tag="U2M88_001076"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEM8373471.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            30560..31507
                     /locus_tag="U2M88_001077"
     CDS             30560..31507
                     /locus_tag="U2M88_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM8373472.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(31718..33790)
                     /locus_tag="U2M88_001078"
     CDS             complement(31718..33790)
                     /locus_tag="U2M88_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEM8373473.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(33723..34100)
                     /locus_tag="U2M88_001079"
     CDS             complement(33723..34100)
                     /locus_tag="U2M88_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEM8373474.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(34229..34807)
                     /locus_tag="U2M88_001080"
                     /pseudo
     CDS             complement(34229..34807)
                     /locus_tag="U2M88_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            34939..36231
                     /locus_tag="U2M88_001081"
     CDS             34939..36231
                     /locus_tag="U2M88_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEM8373475.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(36291..37409)
                     /locus_tag="U2M88_001082"
     CDS             complement(36291..37409)
                     /locus_tag="U2M88_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEM8373476.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(37423..38844)
                     /gene="arcD"
                     /locus_tag="U2M88_001083"
     CDS             complement(37423..38844)
                     /gene="arcD"
                     /locus_tag="U2M88_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEM8373477.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(39090..40211)
                     /gene="mnmA"
                     /locus_tag="U2M88_001084"
     CDS             complement(39090..40211)
                     /gene="mnmA"
                     /locus_tag="U2M88_001084"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEM8373478.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            40615..41214
                     /locus_tag="U2M88_001085"
     CDS             40615..41214
                     /locus_tag="U2M88_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEM8373479.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(41352..42638)
                     /gene="efmA"
                     /locus_tag="U2M88_001086"
     CDS             complement(41352..42638)
                     /gene="efmA"
                     /locus_tag="U2M88_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEM8373480.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIITDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
BASE COUNT        12614 a         8628 c         7145 g        14464 t
ORIGIN      
        1 tgtgtttcct ccttataaaa ttctgttaaa gactaaaaca tacagataat aaaaacaaac
       61 tctttattac tatatgtttt agtcttattt gtttactcaa cctttatacc gttccatgaa
      121 actatcaata tcagattttt taaatctaat gaccgtatca atttttataa caggtaaccc
      181 gtattgagaa acatattttc taatcgttcc tactgataca cctagatatt cacttgcctg
      241 ctgatagtcc atatactcta gagtgactcc agaaataatg tcttctagca taggctttag
      301 cactgtaatt atctctttca cttgttgttc aacttgtata gaaacaatgc tatcaatgat
      361 ttgtagagac atatttcccc ctcctttcgt gattgcttga acaaattttc taagaattat
      421 cttcgtattt tttgctcaaa ataaaagtat tttgttatta accgattaat caaacaaaaa
      481 atgattacca gttcatcgac atacacataa tacctcgata aaaaaggggt gtcaacagtt
      541 ttgtaaaatt tatttacaac accccttttt tgaggtatac taattttagg aggtgtggtc
      601 aatatgataa aacgtccacc aataaattat ttagaacgaa aaaagatact cggaacaaaa
      661 ataaaagcta taagaaagag taaaaaatta acacaaccag catttggtct tatgattaac
      721 aacggtcaat taattgacaa aaagactatc tacgaatggg agaagggaac ctatttacca
      781 ataccagagc gattatcccg aattgctgat ttgggaaata tgagtataga ggagttagtt
      841 tgtggcaatg tagaagaata tattttggga attattcttt atagagatag cattgtacta
      901 gatggtataa catttccaga taaaaacctg ttccagcatc ttcgtcaaca gttcccacca
      961 gtacactcta atttagatac atggctggat agatattcca aattagaacc agagatgcaa
     1021 gaatttattg ctaacaagac ttgtaataaa gttaaaaatg aaaaaattag tcttttcaat
     1081 atcttaaaaa tagaagaatt attcatcaat gctattgtag aagaatttga caataacatt
     1141 cttttcctca ctagcagtat agaagaacta ctagaaagaa tggtagatga atggctacct
     1201 attcaattga aagacatgtc ttatcccgaa gaagctgtga gagaaattac tgataatata
     1261 aacaagctag aacaaacgat ttcatcaatt gggaaaaaat acactaagaa aaaaatgaag
     1321 ggaggtgata ccatctagtg gtaagtactc cttgtaaact aagaggagat gatttcatgg
     1381 caaaaatatc aaagtacaaa aagaaaaacg gtcaagtggc gtggatgtat aaaggacaca
     1441 ttgccaccga tccacgaact ggtgaaaaaa taaatactac gagaagaggt tttagtagca
     1501 aacaagaagc tcaaaaagat tatgacgagt acagacatca aatattatac ggtgttaaaa
     1561 agaaagcacc tgatatgaca tttgaagacc tgtataatga atggataact catcagcgta
     1621 ccagtgttaa agcttcaaca atcgcaatct cagtaagata cgcaaataat caaatacttc
     1681 cagcatttgg taagcttaaa ctatcgaata tctctgtccc atattgtcaa aaagtggtag
     1741 atgaatggca cagtaaatat gaaagttacg attatatgag aaaacagact gcacaaattt
     1801 tacgctacgg ggttgcaatg caatatattg acaataatcc tatggaaaaa acacttcttc
     1861 cacgaaaaaa agagtatgag aaaaatcgta aattttactc aaaagaggag cttaataatt
     1921 tactggatgc ttttaaagat tttggtaaca tgaaacaata cgccttcttc agattattgg
     1981 catacacagg tatgagaaag agtgaagtac tcgctttaca gtggaaagac atagacacgt
     2041 ttaataaaga actacatgtc aataaaactc ttgctgtaga tgaatttggt aaagttataa
     2101 ttcaatctcc gaaaacaaga gcttctagac gagttatctc tttagatact gagactcttt
     2161 ctatcctcaa caactggaaa ttacagcaaa aagaagagta tttaaaactt ggatacaata
     2221 ctagctctaa agagcaacat gtttttacaa cagttaaaaa cactctatac attccaaaca
     2281 ctgttaatga ttggttaaga tatattttga aaaaatataa cttgcctcgt attacacctc
     2341 atgggtttag acatacacat gctagtttat tattagaagc aggagaatct gtcaaggtag
     2401 ttcaacaacg attagggcat gaaaatagta aagtgacatt agacatctat gcacatatta
     2461 caaataatgc acctaagaaa acaggtcaag actttgctga tatgatggct catcagtaaa
     2521 agagctggtc aaaatctggt catttctagt atacaggcaa aacaaaaaag ctagaaccct
     2581 tatatatcaa gggttctagc tcctataaca ggtttaccca acactgcctt ccaatgactc
     2641 ttttgggaat cagaggaaag aatcaggctt tatagagata tttcatctaa caaattactt
     2701 tagactgaca gtatatatca gcaggtactg tcatcgggaa cgtagccaat atcgtatcca
     2761 aaacagcagg tagagaagct atttgaggat tcaagttatt ctatttcaac catgaagtta
     2821 agttcattaa aaataaagat agttggtatt tcaataattt ttgtatagag ttaatcactt
     2881 tattgatgct aattcatctt gattggtatt ataatcgtat tcaagttgcg tggcgtgctt
     2941 ttgctagaaa atatataact gttctttata aagaaatata aggattaaac attttttatc
     3001 atagagtttt acttatctat agaattatgt ctgttcctaa ttcagtttga tgaattaact
     3061 gataaaaaaa tatctactat ttaaagatac agggataagt aagtaaaaaa ataacatgtc
     3121 attagatagt agacagttcc aactctttat ttaccttttt tgtatgctgc actgtcaatc
     3181 caagttccta aattgttgta tccatcatca cttatataat catattggcc atcaataatt
     3241 tcgtcaaacc acaaatattc tccatctggt ttttgcccaa ttattttaat tactgattga
     3301 ctagtagttg tcatctggtt aaaatctttt attttaccta tattaacggc caaaatagca
     3361 tttcctcgct tataactctc ctggatttca tatttaacat attctcgatc aagagtttct
     3421 ttacctatca gcaccacagt cactgatgtt ccaattaatt gattatctat ccagtttttt
     3481 atagcaatat ctccattacg tttaactttc tcaaattcag ccttatctac aaaaccagct
     3541 tcctttgttc ctcgcaaagt ccagctgttt ctaactacca ttgctcttgt tacatctgca
     3601 taatggaaac taaaaaaaac ccttctcacc atattaatcc tcctgaatac tatttattaa
     3661 actgacaatt tgttcaactt ttttatctac ttctccttca gtatctaatt tttctagcgc
     3721 ttgaaattta ctttttaagg aatcatcaaa ataatcactg aaattctctt gaatcttatc
     3781 aaacaaaacc ttacttgcac ctttggtaaa tccaatcgga actactatat tttcattttg
     3841 aacagataat tcaaattctt caatcattcc atctgctgta actatttcac ctgattcaag
     3901 tttgtttcca aaaacaaaaa tactaatccc agactctgca ataatgtttt ctcgatatct
     3961 tctccactca tgaggcatat caaggggaag tggtcttaaa gaaagtacgt cttgtatgtt
     4021 attttttctt aaatttcttc tctgctttag aactcctgaa acaatactat taccaattcc
     4081 aagtccgtaa cctgatacta ttctataacc ggattcagaa agcctatatg ctaatttctc
     4141 gacaaatttt aatcctttcg attcagatgt aaatggagta taatcaacag cacttcccga
     4201 aataaaaata gtttttctca aatacttttt tctaatactt ttcaaaatat cagtaatctc
     4261 agaataatca tctacttcaa caacgtttat cgaaaatctg gacaaatcat tgatttgtag
     4321 tcttcttttt gtcagttcat aattatatct ctcttgatca aaaaaatcct cagaatcgac
     4381 aaaattttcc tttaatattt ttctcattat acaataatga ggtctttggt tttctctcaa
     4441 aacccatctt atatctgata aaattctttc taaattaggg tctgtaaaac taaatcctaa
     4501 aaagagaaaa gtttttgtta acaaatctcc ttttaaagtt tctttaaata acgcgtacga
     4561 atcatcatca tatttttcat aatcatctct tgttattact gtcgaactga catcatctat
     4621 atcaccatgc attttataaa caattgtatc cacatttgga ttagttgttg ctaactgagt
     4681 ataatttttc ttaacctgag ggtttttata agcttctcgg attgattttt ctattagatg
     4741 atcataatta gttgtccaat aagtttcaat aggtaaactt gctagtattc gatgattttc
     4801 agatatttct aattcttcac caaaattatc cataagtatc tgattcaaaa ttcctcttga
     4861 tccaccattt tcatttttat aatattgagc cagagtaact aagtctgttt cttttttaga
     4921 atccaacccg atttctgttg caggatcatt aagtagttcc ggccaactta catatccttg
     4981 tgacatcgat aatcctgcac ctgcaaatat tgctgcattt cctttttcaa tagcttcaga
     5041 gaacctcttt attaaaactt tcttatccat tctacccggt tcctttcgat tcagtaaatt
     5101 gattagagat tagtgaaaat ggtgttttta tataaaatat ctaatgtatt acttccttta
     5161 gtgcattcca cagcatctaa agcccatttt ttcaaatcag cttttcgtaa ataagttaat
     5221 tcacttaaat ttaaagaata ttttatttgc agattttctt gcgaagaata gtgctttata
     5281 atgcttctaa catcctcggt gctcatttga ggattacttt ccaatcgtgg attctttttt
     5341 tccactattg aagctgtcgt cagttcgcta tatagccatg gcgaatacgt aaatttctgt
     5401 tcattagtga tttcattgct caaactaatt gaattaggtg tattaagaaa aaacagacat
     5461 tctgtactat caatcatatt atttaaagct gtagaaagca tcatatgaac atgactagtt
     5521 gttacatttc ttttatcata atcatatgtt tttgtttgtt ccttatagca atatttttgg
     5581 tctatttttt ttaataaatc agaactgtat ccccaaaccg aagaatcaat aaatgcagta
     5641 agaccaaaat tttcaaatag ccatgatgca aaacgtatag ctaatttttc atcagcatga
     5701 gagtgagata taaaaatatc tgcttttata gttggaaacc aatctttaat aatttcgtct
     5761 gcatctagaa ttttgctgtc cttagaataa taatcaacta aatcattttc actttcaaat
     5821 agattttcta ttctcttgtt aatagatttt tggttgtgat ctatttgatt tactacatgt
     5881 gtatatgatt gcgagcttga ggagaatgca tttaagtcag catttgtaaa atccaattca
     5941 tatgccttat acaaggatat ccctccattc ttgtattttg atccacaatt tattaggcaa
     6001 cataacagta taccctatcg ttacatatcc cattcattat tataccagta tttcttcgtg
     6061 acacataagg gtgtaacata ataatttcaa acgaaatgac tacaagtagt cattgaagca
     6121 aatcttcata agacacctgt agaatatctt tgatggcttt caattgatcc aacttaatgt
     6181 gttggcggcc accttcaatc ttaaccaacg tttccctagt tatagcaatt ttacgaagct
     6241 gtaggtctct aatcagctcg gtttgaccaa ttcctttttc taatcggact cgtctaatat
     6301 tgctaccgat tacattttct ttatcaataa ttttttcctc tttcaagaat agcacacctt
     6361 ttggactaaa attagtcctt tttcttatta atcttaaaga ttcggtatgc tacaatggga
     6421 ctagtattag tccatattca attttaagga ggaatttttt tgaagaaatt tgcaatcatt
     6481 ctaacttgcg ctttactcat cggaactata agcgggtgta ccacttctga cacaagttct
     6541 acaagtacgg aaactagtac aacaacctca gaaaagaatt acccaataca tttttttccg
     6601 gttccagatg gatttgaagc ttattatcat gataaaaaac taaatgttcc tttatcagaa
     6661 actgaatcta ctgaaaagat tttgccaatt gaaattgaaa gaaatcaaga cgatataaaa
     6721 ctaaaagctg atcttccctt actgaacagc acacaaattg atagacatga aaatattaaa
     6781 gttaatattg aaaaaggagc gttagcacaa ctttatactg aaaaagttga acaatttttt
     6841 gaggctggtg ccaataaaga cttttcaaaa ataagcgatt attcggataa cttttttaat
     6901 gagacccaag atgaaatcac gaactcaact tttatggctg aagatatgta caatatgaag
     6961 ttgaaaaaaa taaagtcgta tgattatcca ttaaatctaa cattaaataa agggatactc
     7021 tcttttgtat tagcaggtta cgttgactat gaatatacag cagattattt ggagacgccc
     7081 cttcaacacg ataataacga tattaatttt agatttatct acaattcaaa aagtaaaaca
     7141 tggttagttg atcaattgag cactataaat acgggatttc atgaattacc taagcctgac
     7201 aatgaaactg ctttggtcat taaaacattc taaaaaagaa acgttgaagg aataattatt
     7261 ctttcaacgt ttcttttatt gtttttcagt ttacggtctt acctgagcga ataatctgat
     7321 ttctaaatga ttggaaaact tcttgtggtt gataataatc tgtatctatt tcaaatgttt
     7381 ctgagaataa ttcattgaat ttttctactg aaatgatgat tttaccattc tcctctcgac
     7441 tgttttctat tatttctgcc tctaattcaa ataaatctag tgacgaacta aaatcataaa
     7501 tcctgtcata ttgaacaaaa aaatcaagaa aaagttgctc aaactcttcc ttatttatca
     7561 ctagtgtttc aagcggaaca actttatttt catcagccat tcttccaatt tcctcacttt
     7621 cattttcaat tgtccattgt tgcggacgta tcagattttc tctatcattt ttcagtgtat
     7681 agatcacttg taatacacaa caatgctttg taaacttatt tttttagtta gtcgaatctt
     7741 tttaaatgtg cgtttcatac catgcgcttg atatattttc tcctattttt taaatcgttt
     7801 atcacaactt ttatcgtttt ttcttctgaa aaaaatcttg tttacatcgt tacatagttt
     7861 ccaaaaagat acattactag tagaaacaac aagattaatt ttacttccca ctgaaatggg
     7921 aagtatattt taaatatttt ttctccttca acttacacta taaagtatct taatcacacg
     7981 atatgctagc cactaaattc atcatatttg attccttttt taagataatg tagcatcttt
     8041 ttatccaata acgcaattgt ttttaaggat aaatttctta agatggtaca tttcaaaatg
     8101 gcatttgaca tatgaaacaa aaaagaaaga aaagagcata agggaaaact gatagcacct
     8161 tcctacagga aggtggctaa gttggctgtg ccaactggtt ttctttcaaa atcatttcat
     8221 aatttttgat atcataaaaa aatccatttt ggacctattt tcggtcataa tttagtacct
     8281 acttttggtc atagtttcgt ccgtagtttt ggtcatagtg actaaggctt ttctgttcac
     8341 aaaattggtt taaatttgct ttctttaaca cttcatttat atcatagcaa ttataatgtt
     8401 tacacaacat cattcctaat tttaacggtt gttcctcttt atatttttta agttctacat
     8461 ttttttgcgt ttaattactt ttctcttttc tttatattcc gctccaactt tctctttaat
     8521 agtgataata gaattacata tttttgttaa acttccttaa aaaaattgga tctcgattac
     8581 tcaaatttgc ttgcaggctt ttctaattgc tttgtttttt cttcctaatt atttgatttt
     8641 atttccattt tcttctagtg aaaatttatt atttttaata atcgtacgcg tagaactgat
     8701 ttcatttttt gtatctgttt gattatctaa cgtttgaaat tcaaagtttt gattggcttt
     8761 atcaatatct ttttatacta ctttatttgc aagatatatt ttcttgctta atttggtcat
     8821 agtttcattc gtcacttcgg tgatattttt ttaatttgca aataaaaaga gttaaaccga
     8881 tatacattag gttcaactct tttgcaggtt tcatgggtgt actatcactt ttaatctgat
     8941 agatgtaaac ctctctttat ctttttgtta cttcaatttt caacaatagc tcctttatag
     9001 gttgtcaata ttgctctacg tattctggct ttttaaccca ttatttctct aaagtggaag
     9061 agaataatcg cttaatcccc actaattcct tgtcgtaaat tcatgtcaaa tacaattttt
     9121 aaaagccatt taattcatgg attatctctt cacttttttc tagataacaa tatttcaagt
     9181 tgttttttat ctcatgtttc atctttaatt cttcttgaat catcagttca atataattag
     9241 tgaccgtgat tttttcattt gttgcgttct ttactgattc acataattcg tcaagttcta
     9301 agaaatcatg cgtactaatc tttagaatta atttcatgac aattccccct tttatttttt
     9361 taatagatta tcgaaaagcg gattaggatc attaagtgta tctctgaata actcgttgtc
     9421 ttcataggta aatcttcgtc ttggttttga tcccttatca ttttcacgga tttcactatc
     9481 aataatcact gtttcatgat ttgaatcaaa ttttttcgct gactttctgt cttcaacgat
     9541 attgattttt tgtgtttttt ctttcatttc tccactatct ttttgaacgt tctcccgaac
     9601 atctactgtt ttgaatcctt gactacttgt agaactttgt acattctctc ttacatccat
     9661 tgatttaaac ccttgactac ttgtagaatt ttgtacattc tctcttacat ccattgattt
     9721 aaacccttga ctacttgtag aattttgtac attttctcga acatcaattg tcttagtacc
     9781 ttgaccattt gaagagcttt gtaaattctc tcttacatca acattccgtc cgacatgacc
     9841 attcaatgaa ccttgaatat tttctcggac atctactgat cggccacctt gactacttgt
     9901 agaattttgt agattttctt tgaccaattt ttctaatccg gtatttgagt ttttagcttc
     9961 ttggattctt tctttggtca acgcttcttt ttcgtccgta ttagctcctg tacgcccttc
    10021 tacacgtttc cttaacattt cctcacgatt cattgcttca ggcgtgtatt tagcttcttg
    10081 tcgcttctga ttaattaatt cttcagcaga cttagcttga ccgtgcatag catttttacg
    10141 ttcttcaata tcttgttgaa ctttttgctg aattgcactt acaggattat tattcgattc
    10201 agcattattt ttcattgctt cacgcatacg ttcagaacta gacataattc tatcggcatt
    10261 ttcaacctta gcaccatctt ttctatccga aagacgtgtt ggcatggaat cagcatattt
    10321 tccagtttca tcttctttat ttttagctaa cataggtttg ctatttttat taatagcgga
    10381 cactgtacca tcatctaaac cttctttgaa cttattcttg gtatcattag ccatatcacg
    10441 catactctta aatggtttta ttgctctttc actagccatt gtagctccat ctgaagctaa
    10501 acctgataat ccacgttcat gggcatatcc caaagctctt ccagttttgc cagctttttt
    10561 tgatagagtt tcgccgaaat tcttttctgg ttttcgatct tctccttctc cactgtctga
    10621 tccatttcca tttccgcttc ctttagcacg attaaatcca gttgatcctt ttctaaacag
    10681 cattgcgccc ataccaaaag cagaagcaag ttgcccatag ccttctttga gtccaatatc
    10741 aacaccaaag tatctcatga ctgttccact gccttttatc agcacaaaca cagcacagat
    10801 ataagcaatt gtttttataa aaatatttgt ggagatacct tgtccagcat ttaaaaaatt
    10861 gataaacata gcaaacatag atagtccaaa cccttgaaat ccaactgtta agtaacattg
    10921 taaaatatcg gataaaacaa cttttgaacg ttgtcctgtt tcaatatctg tcgcaaaaac
    10981 aagaacacct aacacacgtt taaatgcaag ttctaatatt gctgtaatga tcacaaacgc
    11041 gctgaacaca taggcgattg ccaacgctgt caacccaatc gaaatccccc aaagatttgc
    11101 ttgatatcga taataacctg atttcaagct atcagaaaat gttgataaga agttatcaga
    11161 aaactttgtg gcaacaaatt cattattaga attttcaact aattcatagc gaagattatc
    11221 cgcagctgga ttttcttttt ccaatttatc aatcacttta tccgtcaaca cttgtgttaa
    11281 atctgtctgt tgaaaatttt ttcgtgttaa cttatttcga tcctccgttt tatcaatgct
    11341 tgagtattga tctgttttat taatatatgc cagatctgtt actccttgtt ttatcaatga
    11401 ccaagcgact ccgtcatctg aatttgtaat cgtagtagca tcattgtaaa atattttggc
    11461 aaattgaata ccgctagaaa tcattgttgg cattaaaaga attaacgcta cagacatcac
    11521 tatattcatt ccaacagatc ttaaatctat tgttcctttt ccagtaatca tcttgtagcc
    11581 aacaaacatc aatgaaagga tcattaatcc aacgacaatt gtatttatga ctgattgata
    11641 aacattattt agccctgtac tttcaatgaa attgaacaaa tccaacacat ctggaatcat
    11701 attttgaatc gaatcagtca cagtaaacat gccttttacc acagcccatc caagccaaat
    11761 cccgaactga ctaaggaaat ctccattgta tttttttagg taaggtctat aaagagaatt
    11821 attcaaaaac tctgtttgac tttttgtgtc atcaaaccaa ttgaaaatgc tagcttcaac
    11881 aatattactt gtaaaaacaa cgctaatcat taaaacaagt acactgagtg ctaaccagtt
    11941 tatttttcta ctaacgattt tttctttaat tgctttcata gttttctcct tcagcccatt
    12001 ttcttaggct ctcagagagc tgctgagcca ttttttgatt aattatgata tgagttacac
    12061 aattgtcatt ttcagctctt aaatcgagaa ctactgtgtt gaagcagata ctctcgatta
    12121 attcgagagt atctgtattt tgatcaagac accacatttg atctattaag cgatttttaa
    12181 tcatgattct tctcctttca accaattatg catagtaatt tcaggtaact ctttgatttc
    12241 gaaagctttt ttgaactgat ctataggctc tacattttct aaaaattgac ttttatatct
    12301 tttctgaagt ccattcaaaa aatatgttac ataatcgtat aacccttctt cagtttttct
    12361 ttgaaaaaag catgcttcat gttgttttat aatacactca gcaataagat actgagtatt
    12421 ttcagataaa tattcaatga tttttctgat atcttctgta tctacattag cgtttctcaa
    12481 atagtgatac acttgaaata attttcgacc atcttcgtta tttgcgtcat aaagaacact
    12541 aatcttactg taaatcctct ttatattaat atcctcttct tccataacga gagtagtctt
    12601 tgatatgttc tgtgaagtag tctttgtata cgtatataca aatttgtaca tacctctacc
    12661 tttttttaaa gagacatatc tctttttttc gatacccctc tgacgaattt tcggcatata
    12721 ctcctttttt tcgcaatcca aagtaggaaa agtaatttct atcaatttat gtacatttaa
    12781 aacaatgtac ataacatttg ataatgtaac tgctcttttc tcataataaa tatttctaaa
    12841 aacccttttg ataatcccct ttttctctaa gtacacaatt gcatctttta tagctcgttc
    12901 tgataatcct gtttttttgc ttaattgctt atatgatttt tgcaaaatat cttcaagaaa
    12961 ctttttttga taaccaagta gctgaccatc atcgttaaaa atttttttag gtctatacca
    13021 ataggtaatt tctgctaaga taactattgc attcaaatag ggcttctcaa cacctttttt
    13081 cgtttttttt cttaattgat cgtaccatgt gtgtggatta catttccgtc aaaatttatt
    13141 tctctaagtt tgtcaactac aacatttcca gaagaaaaca ccttttcacc ttcttttttg
    13201 atataatagc tataaataaa gttttctcag cactttatta ttgagtgttc ccccagtctg
    13261 caaacttttg ggaacacttt ttttcttttt ttaaagcatt tttttataat ttctacttca
    13321 tcatcttcca atattttccc ctcttttcat tcgagcaaac ataaactcat taactgcctt
    13381 tttcgaataa aattttcttg cccctaattc aactacgggc aatccctcat ttctcacagc
    13441 attttttaaa aagttttcac tcacatttaa gtattccgcc atttcttttt gattgagata
    13501 ttcccttgtc atccccgcat tttcttgagc cttttcaata gcttttgtag caatttgata
    13561 aaactgcttt agaaacattt cctcctgttc ttctgaaatc attaatttta ttgcttccac
    13621 aaaaacctct cctttttaat taattattct ataactcctc tacctttata agtacaaata
    13681 aatacaaatt gcgtaaaaaa atgtatttat ttgtatttat aagaataaag tatcatactc
    13741 cagataaaaa gtaaatactt ttaaatactt ttattttata aatatgatat actatcttta
    13801 aaaataaaaa ggagaaaaaa gatgaagcca aatccttcag aagcaggaaa acgaatcaaa
    13861 caattacgcc tttcatgtgg ttttactatg gaagaacttg gaaggaaaat agataattca
    13921 ccacgtgcga ccatatccaa ctgggaacgt gggaccaatt taccaaatcc acaaaagctg
    13981 aaattattat caactatcac taattcaaca attgactgga ttaaatgggg aactttagaa
    14041 gaatatataa cttcatacct tatcgatata gggtatgagc tatatattaa ggacttccca
    14101 gaaatacctc acaaagtctt taaagacatt caagaaagat atagtgatac gttttcgcta
    14161 gataaagact atgagttatt gaatcctata ataaaaaata tatttacaaa atattactca
    14221 aaagattttg aaatttatct aaatgagata cttgctaata atatcaatcc tctgattgaa
    14281 gagtacgcta accaaagaca ctctatctct aaagagctat ttttcaaaag agccaacagt
    14341 aaacttcaag acaagataag aaatggtgat ttcaaatacg gtgaaaaaga aagaatgatt
    14401 gattctttaa aacagctatt agacgaaatg gacttagcct ataaagatat ttcaaagtat
    14461 tcatctgtcg aggatttctt ttataaaatt actaaaaacc aatttgaaac agaaaaattt
    14521 atttcagatc ttagtaaaaa atacaatttc ccatatgaga aaaatagtat aatcgcaaaa
    14581 tttttaactg ataatcacga taaatttaaa aataattgat tcttcaaata acttctctac
    14641 ctgcaataat gaagtaagga gagaatcaaa tggcaacatt ttttagttat aaattacaaa
    14701 atggcgaaaa atattgggga tttgaaactt atttagggaa agatcccaag actggaaaag
    14761 atatacgtac aaagcgtaga agattcaaat caaaagctga ggcgcaaaca gcacttaata
    14821 gactacttgt agactttaaa gaaaaacaag agtttaaaat caatgttaca acttttcaag
    14881 agttgtatga attatggatt gataactaca gaacaagagt aaaaccgtct tctgttgccg
    14941 ttgctcaacg tttttgcaaa aaccatatat tacctgcttt tggggaatta cgattggata
    15001 aaataagtgt tagctattgc caaaaacagg tgaatgaatg gcacaaaaca ttaaaacaat
    15061 atggtttctt gagaaaaaca actgctcaaa tatttaaatt tggagtagca atggaagtct
    15121 gtaaggataa tcctatgtct aaaacattgc ttccaagaaa aattgaagaa gaacaaccct
    15181 tgaagtttta cacgaaagat caattacaac tgtttttaca gaatactaaa gaaaataatt
    15241 cagttaaact gtacactttt ttccgtttat tagcttatac aggaatgcgt aaaagcgaag
    15301 cattggcgct tcaatgggaa gatatagact atttcaataa aacaataaca attggtaaaa
    15361 ctatcgctca agacgagttt aatcaagtag ttctacaagt acctaaaact aaaaattcat
    15421 caagaacgat tcagcttgat gattttacac ttaaacaact gcgtatttgg caacaagaac
    15481 aaatgaaaat aatgatctta tatggctata acacgaattc accaaaacag tttcttttca
    15541 ctacaaacac aaacaagttg tactaccccc aagtagtaaa cgattggtta gactggattt
    15601 acaaaaagac accgatggag cctcaaatta ctccacatgg atttaggcat acccactgta
    15661 gcttattgtt tgaaagtggt gcgtcgataa aagaagtaca ggaacgatta ggtcataaag
    15721 atattaaaac taccatgaat atctatgctc atgtcacacc acaatctgtt aagaaaactg
    15781 gtgaccgatt tgcaaaattt atgggcgaat aattccaaac gtagccaaaa acgtagccaa
    15841 ccattaaaat acaaataaaa aaacgctcga aacgctttaa tatcaacgtt tcgagcaatg
    15901 aaatagttat taacctacag acccttccat ctcataactg atcaatcgat tgagttcgac
    15961 tgcatattcc attggaagtt ctttggtgaa tggttcaaca aagcccatga cgatcatttc
    16021 agttgcttca gattcggtca ggcctcggct catcagatag tagagttgtt cttctgagat
    16081 ttttgagact ttggcttcgt gttctaaaga gacttggctg ttgtggatct cattgaatgg
    16141 gatcgtatcg gatttcgacc attcgtccat gatgatcgtg tcacattcga tatgagagat
    16201 cgaacccgca ctgtttttgg caaaggttac ttgtcctcga tagtttactt ctccgccgtc
    16261 ttttgaaatt gatttagaga cgatagagct agatgtattt ggtgcattgt ggatcatttt
    16321 tgcacccgta tcttggattt ggtttgctcc tgcaaaggcg atggagagca tggtgcctcg
    16381 tgcacctttt ccatctaagt aaacacttgg gtatttcatc gttgtttttg cacctaggtt
    16441 tccgtcgatc cattcaacgg ttgcgccttc gtatgctttt gctcgtttgg ttactaagtt
    16501 gtacacattg tctgaccagt tttggatcgt tgtgtaacgg caatacgcat cttttcttgt
    16561 aaagatttct acgatggcgg catgcaggct gttacttgaa taagtaggtg ccgtacaacc
    16621 ttcgacatag tggatgcttg ctccttcgtc aacaatgatc agcgtacgtt cgaattgccc
    16681 cgtgttttcc gcattgatcc ggaagtacgt ttgtaatgga acatcgactt tgacgccttt
    16741 tggcacgtaa ataaaggtcc cacctgacca tacggctgaa ttcagcgctg ctaatttgtt
    16801 gtcggttggc ggtacaagtt ttgaaaagta ctctttgaat aattcagggt attctttcaa
    16861 agcagagtca gtgtcagtaa agacgattcc taacttttcg aattcatctt tcatattatg
    16921 gtacacgact tctgattcat attgtgcgga agcaccggct agataagctc gttcagcttc
    16981 tggtatacca atcttttcaa atgtatcttt gattttttct ggtacgtctt cccagtcacg
    17041 agcgggtttg tcacttggtt tttggtaata tttgatggca tcgaaatcga tgtctgaaag
    17101 gtcggggccc catgtttgca ttggcatttt gttaaatgtt tctagagatt tcagacggaa
    17161 ttcaagcatc cattctggtt cgcctttgat tcttgacatc tcacggatca cttcttctgt
    17221 caagccttct cctgtactga aaaccggttc gacatcatcg tgaaacccga atttatattc
    17281 ttctaattct ggtacgccca tgttctcact cctcttcctt gtggtaatga tggaaagctg
    17341 cggtaccatc gttttccatt gcttttccta gtgctttcca ggcaagtgtc gcacatttga
    17401 tccgagcggg aaatttagct acaccaccaa gcatagcagc atcgcctaat tggtcgggat
    17461 ctttgacttc ttttccttgg acaagctgta gaaagtctac agttaaatgt tctgcttctt
    17521 ctattgattt tccgatgacg gcgtctgtca tcatcgaggc acttgccgta ctgatggagc
    17581 agccactgcc gtcaaaggcg atatttttga tgattccgtc ttttacatcg acttgcaatt
    17641 cgatcacatc tccacatgtg gggttgttca attcgatctt gcttgtcgag tcagtcaagc
    17701 ttccacgatg atgcggatgg gaagaatgat ccaagatcac ttgacggtat aaattgtcta
    17761 atttagatag tcccatgttg gaaaaactcc tttgttgcca tgattgcttc aactaaacgg
    17821 tcggcgtctt gcgttgtgtt gtagaaataa aagctcgcgc gagcagtcgc tgatacttca
    17881 aggtacttca atagcggttg cgcacaatga tgacctgctc tgacggctac cccttccatg
    17941 tctaaagctg tcgcgacatc atggggatgt aacccatcca aattgaatgc aacgactcct
    18001 gtccgaaaat tcgggtcttg tggaccataa acagtcaatc catcaattgc ttgtaatttt
    18061 ggcaacacat aagcggctag ttcttcttca tatcgatgga tattgtccat accgagtttt
    18121 tgcaaataat cgattgcatg gcctaatgcg atagcgccgg cgatatttgg tgtccctgct
    18181 tcgaatttcc aaggtagttc tttccatgta ctgtcgtata attcgacaaa atcgatcatt
    18241 tcgccaccaa attcaacagg ttccatttct tctaaccatt ttcgtttacc atataaaaca
    18301 ccaatacctg ttggtccgca cattttgtgt ccgctgaaag caaagaaatc acagtctagt
    18361 gcttggacat caatggccat gtgcggggca gattgagcac catctactac gatgaccccg
    18421 ccgttttcat ggacaagttc agctaattct tgaacaggat tgaagacacc taagacattg
    18481 gatacatgag caatggagac gattttggtt ttgtccgtaa tctttttcgc tgcatcctcc
    18541 atatccagga agccttcttc tgttagttcg atatatttca agacagcacc tgttcgtttt
    18601 gccaactgct gccacgggat aacattagaa tgatgttcca tgtaagagag cactatctcg
    18661 tctcctgctt tgatatagcg gtcgccgaag gttctggcta cccaattcaa gcttgtcgtc
    18721 gtaccacgag taaacagcac ttctgctgtt tcttttgcat ggatgaacgc ccgtactttt
    18781 tctcttgctt gttcataatc atgggtcgcg cgttctgcta acgtatggac accgcgatga
    18841 acattggcgt tatcgtgttc gtaatagtac gctaatgcct ggagtacttg ttttggcttt
    18901 tgggtcgtcg ctgcattatc cagataaact aaaggttcat cattgacgtc ttgaaataag
    18961 ataggaaaat cattgcgtag ctttttagca tcgatcatcc attcaacttc ccttcgatta
    19021 cttcgactaa ttctttccga acttgttcta cagggattgc tgttaagaca gaaccgagga
    19081 atcctcgaat cactaagcgc tctgcttctt ctttatgcaa tcctctactc atcagatagt
    19141 acatttcttc tggatcaact cgtcctacac tggcagcatg tccagcagtt acttcgtttt
    19201 catcgatcaa aagaatagga ttcgcatcac cacgtgcttt atcagaaagc atcaagacac
    19261 gactttcttg ttgggcatct gcaccttttg cccctttcaa gatgtggccg ataccattaa
    19321 aggtcaatgt tcctttttct cggatcactc cgtgttgcaa aatatgcccg atcgtatgag
    19381 gggctttatt ggttactcgt gtatcgattc cttggatttg tctgcccgag ctgattgcca
    19441 cgattttgac ttctgcgtgt gcaccttctc ctgctaaatc tgagtcaaag tcagcgatca
    19501 catgaccgtc attcatgaca ccaattgccc agtcgacaga agcatctcgt aaaatgtgcc
    19561 cacggcgatt catataagaa gtaatatttt ctcctaattg atctacggct gaatatttga
    19621 tttttgaacc agctttagcg atgacttcca cgatgatatt acctgatgat tttgctactt
    19681 gttctttggt tgtctggaaa cgttccagat aagaaaattc actatgttca tctgccacga
    19741 tcaacacatg cttgaagaag tgctcatcgg atgcgccatc ttgaatgaat aaggactcga
    19801 gtggttcatc gatcacgaca tttttaggca catacaggaa aataccgctg ttcataaaag
    19861 ctacatggag tgcagtcaat tggtcttcgt tcatgtttac tgcttttttc atataatatt
    19921 cttggactag ttctggatat tcgatcattg cagtgaacaa gtcggtaaaa atgactcctt
    19981 tttctgctaa ttccacaggc agttgttcaa aaatcgtcca tgaaccttgt tggacaagca
    20041 caggattgtc tttcattgca tcaaatgccg gaatcgtgcc tgtttcactg atggtttgtg
    20101 tttctttcac atcaaataac gaccagcgat ggaatttcac ccgatcgata tgcggcaatg
    20161 gcagttcatc tgctttcgct agtgcgtctt gacggaaggt tctcatccaa tcaggttcgc
    20221 ctttacttaa agaaaaatca tttaccgcgt caagataatc gagccagttt ttttctttca
    20281 ttcttgctcc cccttacgct tcttcttctt tatagtcgat tcctaattct ttactgatac
    20341 cagcgtagcc ttctgcttcc agacgtttag ctaggtcagc attccctgtc atgactactc
    20401 ggccttccat catgatgtga acgacatcag gtgtgatata gttcaataat cgttgatagt
    20461 gggtgatgat caatgcaccg aaattgtctc cgcgcatttc atttactcct ttagcaacga
    20521 ctttcaaagc atcgatatcc agacctgaat cgatttcgtc taaaattgca aatgtaggtt
    20581 ctaacatcaa caattgcagg atctcattcc gtttcttttc tccgccagaa aaaccttcgt
    20641 tcagataacg ttcagccatt tcttctggca tatttagtaa agccattttt tcatctaact
    20701 ttttcaaaaa tgccataaca gaaattttgt cgttttcatc acgtttcgca ttgatcgctg
    20761 cacgcatgaa ttccgcattc gtgatacctg gtatctcact tggatattgc atagctaaaa
    20821 ataagccaag tcgcgcacgt tcatctactt ccaagtctaa aacatttttc ccgtcaaaca
    20881 aaatttcgcc ttctgttact tcatagtttg gattgcccat gatagcggca gataaggtag
    20941 attttcctgt gccgttaggt cccatgatgg catggatttc tccggttttc attgttaggt
    21001 ttacgccttt aaggatttct ttttcctcga tcgacacatg tagatttttt atttctaaga
    21061 cagacatgca ttataccccc aaattctctt tcgttctcac tcattttaga ctaattgaga
    21121 atgaagcgca atgaccttcc ttactaaaat caaatatctt ttatttatta ttaaacaatt
    21181 ttgatttcta aattagaata ggtataaaaa agatgagaaa taagccatta cagacttatt
    21241 tctcatcttt tttgttccat accttatttt gcatcagcct ttactggttt aacagagccg
    21301 ccccatttgt ctgtgatcca ttcttggact tctttgcggt gaagcacttc aatcagtttt
    21361 ttgattttgt cactgtcttc atcaccttta cgagtcgcaa taatattcac gtaaggagag
    21421 ttgtcctttt caagcaatac ggcatcttct aatggattca accctgcgcc gtaagcgaag
    21481 tttgcgttga ttgcaactag gtcaccttca tcattttggt acgtagaagc taaaagtgat
    21541 gggtcgatcg tatggttgaa cttcaagttc ttagggtttt cttcaatatc atcgaatgtt
    21601 gctgtttcca gatctactcc atctttgact ttgactaagc cagcatcttg tagaattgtg
    21661 atgatgcgtc cccagtctga ttcagaattt gaagtaatga ttgttgcacc atcttttaat
    21721 tcggaaacat ctttgacttt tttagagtac aagcccattg gttcgatgtg gacagcaccg
    21781 gcattgacaa agtcatatcc tttttctttg atttctttgt tcaaataagg aatatgctgg
    21841 aagtaattgg catcaatgtc gccttcttcc aaagattggt tcggtaatac atagtcatcg
    21901 aatttgacga tttcaagatc gattccttca tctttcaata atggtttcac atgttccaaa
    21961 atctctgcgt gcggtgtcgg tgaagcgccg actttcaatg tcgtgtcgtc tgctttattt
    22021 gctgaatctt tgctgtcgct gttgttaccg catgctgcta atcctacagt cagcaaaagc
    22081 gctgctgcaa atccaaaaat tttctttttc atatcctaca tccccctata tcctattatc
    22141 ttttgtctgt tttcttagtt aagaaatcac cgattccttg aacgataaat acgattagta
    22201 aaataattaa agtcgcaacg aatgtcactg ttagattacc acgctggtag ccttcctgcc
    22261 aagcaagacc tccaagtcca cctgcgccga tcgcgccggc catcgcagta aatccgatca
    22321 tcgagatcgt tgtaactgtt aatccagaaa tcaatgcggg aatactttca ggtatcaaga
    22381 ccttccagat gatttcccag taacttgctc ccattgcatc tgctgcttcc agtacaccag
    22441 ggctgacttc acgaaaagca atctctacta gacgggcata aaatggtgct gcggataaaa
    22501 caagagctgg aatggctgct tgcgcaccta gcatagtgcc tacgattgct ttagtaaatg
    22561 gaatgatcaa aaccatcagg atcataaatg gcgttgaccg gaaaatatta ctgatgatcg
    22621 atacgattcc ataaatgatt cgagcatacg gtttgttttt gcgtccaata gagtaaagga
    22681 tcaatcccaa aactaatccg atgatcgtca ctaagaccat tgagattaat gtcatatata
    22741 gtgtatcaag gatagcctga ttcatcgttt ccgggttgat ttgtttaaaa tctagatatg
    22801 tttctgaaaa gcttttatcc acgatggatc acctcaattc ctactccttg ttctttgatg
    22861 aacacttctg cttgatctaa ttctttggtt tcaccagtca tcaagacagt caatgaacca
    22921 aaggaacctt ctttcacttg tttgatcttt ccatacacaa cgttgacatc gatgttatag
    22981 cgtcgaatcg cttgggaaat gactggctcg ttggcatttc tttcgctaaa ggtcaaagtt
    23041 gccaaacggc cgcttggatt ttctttgatg agttcagcta atagttctgt cgaatcagct
    23101 tctggttcga tgtcttgttg tacaaagcgt ttggttactt cttttttcgg ttgtctgaag
    23161 acagtcaata cgtctccttc ttctacgact ttccccaact ccatcacagc gactttgttg
    23221 cagattttgc ggatcacatt catttcatgt gtgatcaaga cgatggtcaa attcaatttc
    23281 ttattgatcg ctaacaataa gtctaatact tcttctgttg tttgcgggtc caatgcgcta
    23341 gtagcctcat cacaaagcag gatctcagga tcatttgcca atgctcgtgc gataccgacg
    23401 cgttgtttct gtccgccgga aagttgtgaa ggatatgctt gtccgcgtcc ttctaacccg
    23461 actaaacgca acagttcttc tgcccgttct ttacgtttgc tctttggtac gccagctaat
    23521 tccagcggca gctggatatt ttcaagtaca gttcttgacc ataacaggtt gaaatgctgg
    23581 aagatcatac cgattttctt tcggaatgta cgtaattctt tgttttttaa ttttgtaata
    23641 tcttgatcgt ttacgatcac ttcgccgctc gtgggaagct caagtccatt caggagacgg
    23701 actaatgtac tttttcctgc gccagaatag ccgacgattc cataaatatc ccctttttca
    23761 acgtttagtg aaacgtcatt gacggcttgg atgtttccgt ttttcccatt gaatgtcttt
    23821 ttgacattcg ttagttggat taatgacatg ccattcacat ctttctctta taatttttca
    23881 aacaaaaaag ccttcgccct cttgccttaa gcaaaaggac gaaagcttga acttccgtgt
    23941 taccaccttg atttaccgtt acttcgcaat aacagcctct tccagtactt ggacttagct
    24001 tctttccgta tactgtcgcg ctgtaacggg cactcccgta gtaagtttgc ttacgctcgc
    24061 ttactctgct ctaagaccat cttccatctc attttcgtta cccattttca gcaaacaggg
    24121 ctctcttcaa acgtccgtga catgtactct tcttttcaaa gctttctcgt ttcatatagg
    24181 attgcttatg attatagcaa ctggaagaat cagtgtcaac ttttttcaag tgattttctg
    24241 attttataat tcgtcaaatt cttttggatc aacatctttg aaacttaaga tccacgcatt
    24301 catttcatct gaatcattca agatattgat atcttcaccg attttatcat taacagaaga
    24361 aactgtaccc gttagcgggc tggcaaattc attgaccgct ttttccgctt caacttcgat
    24421 cagaggttca tcttttttta gcgattgtcc tgcttttggc agattggcaa acgtgatgtc
    24481 tccaagttct tcttgcgctt catttgtcaa accaacacag tattcttttc cattaaataa
    24541 gatccaaagg ttgtcctttt tcttcaaatg cttgcctgcc attttatatc agttctcctt
    24601 cataaatttt ttcattgaat ccaattgcaa caagctggtc ctctcgtaag atcagtggtc
    24661 gcttgatcag cataccatct gtcgataaga cttggctcgc ttttgaagcc gtaaactcgc
    24721 ctacttgatc tttcagtcca agttcccgat atttcatccc gcttgtgttg aacaggcgac
    24781 ggattggtac ttgactcgtt tctagccaag tctcaatcgt tgaggccttc ggtacttctt
    24841 tgatcatatc aacggtttga taactaacgc catgctggtc taaccacgct ttggcttttt
    24901 tgcatgttga acatttcggg taccagtaaa aggtgtacaa ttttcttacc tccttgaaga
    24961 tcttgcaggt gcttcatttt ggtatctcat cgataatatc aacccgatac cgatcatgtt
    25021 ccctaaaatc gaggatcctc cctgactaat gaaaggcaat gggatccctg ttaaaggaag
    25081 taatccgata tttgctccaa tattctcaaa gacatggaac agcagcatca tgatcaaacc
    25141 gcttgcgata taggcgtaaa attcattatt cgtatcgaaa cacacccgaa tcatacgata
    25201 gattaaaata aaatacaaga aaatgacgaa tgcaccaccg ataaagccga agttttcacc
    25261 gataacggta aaaatcatat cggactcacg aacaggcaca tagatattgc ttacgttgaa
    25321 tcctttacca aacaagccac cggtaccaat tgccaaaagt ccttgggccg gctgataact
    25381 cattcctgac gtatcatgga aagggtccag ccatgaatca atacgtgcaa actgataaga
    25441 tttgaatcca actttataaa gcaagtctct tccgccttca gtcgtaacca agaagatcgt
    25501 tcctgctcca acgactactg ccaagattac tacaggtgcg atgatgcgcc aagagattcc
    25561 agacatcagg aaaatcccgc caaagatcgc aagaaagaca agcatcgttc caaagtcatc
    25621 ttgggccatg atcaatgcta aaaccggtat tgtcacgatc aagagcttcc caatcagcca
    25681 aaagtccgat ttcatcgtcc gttcttttac ttgcgtatta tgtttagtta cgatcagtgc
    25741 catcatcaag atgtacgcaa tcttcatcaa ctctgctggc tggaaagtaa acgcaccgaa
    25801 tcgaaaccag tttttagacc ctgttgaagc agctaaattc tgatcataga atttgagcaa
    25861 gagtcccatt accactaacc ccaaaccata aaggtaagga gtcagtttcc agatccattt
    25921 ggaattcaaa tgcatgatga caacgattgc tgccgctcca actaaatacc agacaacttg
    25981 catccctacg cctttcaaga ctgctggatt gttcggatca tggctaagtg ctacatataa
    26041 tgagagcaga ccaatcaaac ataacagaaa gactggtaaa atcacgccat aatcaatacg
    26101 attatcgttc gaagctttta attttttttc catgtttcat cccttttctt aaaaaccata
    26161 ctgctgatta ttatagcggt ccagttagat aatggaaagt ctttccatag acatttggga
    26221 aaaattaact ttccgtttgt gcagttgctt gagcaaatgc agtacaaaag cctactgctt
    26281 atctttagtg tatcatagaa ctttttttga tacatgcaat tatctaccag ctgagattct
    26341 cctcgctgtg agaagtttct gtgctttcat tgtatcgaag gatctcttcg gcaatctcta
    26401 gcagctgatc agaaaccaga ttggttcgtt ggatgaaata aaaatatcga tggaaattca
    26461 acggagtcat tggcacattt tctggaacag agcgcttaga caaaatcgag cgaccgattc
    26521 cttccttcag caacgcaaca attacatcgt tacttttcac gatcatcttc ttatcttgga
    26581 tattcatctc ttcaaaataa cgctgcgtgt aatgaaaaac accagagttt gcttctctta
    26641 caagccacaa tttgcttttt gggtccccag cgatgactag ctggtcttcc atcaaagagt
    26701 atctttgtac gccggaagtc tctaatggct tttcgataaa accaaaatcg gattcatgtt
    26761 tcgctacttg ttctaatgat tcatgagaat tcgtgatttc gatctcaaaa tgcactttag
    26821 gaaacctttt gatcagatgt ttcatcaact gaggcaaaag atagacagca aaggtgtggg
    26881 aagcaacgat ccgacatact tcttcttcat tttgttcatt gagcaatttt tgctgagttt
    26941 cttcccattc ttctaacaaa accagtgtcc gttcatataa taagtcggct tgcttagttg
    27001 gaagcatctc ttttcttccg ttacgttgaa aaagatcgat attcaattcc cgctccaact
    27061 gtttgatttg attcgagact gctggttgag ataaaaacag tacttctgct gctctggaaa
    27121 aatttcttgt ttcatatact gctcgaaaag tcgttaataa cttaaacatg gcaccacttc
    27181 cataactttt tatgataatt actataaaaa ctatttgatt taaatataca aaaaaccatc
    27241 tataatagaa atgtacaaca aaacagttgc tgatccaact agtaacttag atgaagacaa
    27301 tcagctgtct gcttgctcgt aatcatacat tcctattcat tttacaatgt attcgttcat
    27361 cttggtacta aaatctatta aataaaaagg agaatattat atatgaagaa ctttttgaaa
    27421 attattccga tcccgatctg cgggttgatc cttgggttag cttcccttgg aaatctgttt
    27481 aaagactatc accatgttgc tttaggcaat ctaactggcg gtatctctat gattcttatg
    27541 atattcattt ttgttaaatt gtttatctta ttcgaacaca cgaaacaaac gttgcatgat
    27601 ccaatcatcg cctccgtttc accaactttt acgatgtcac tcatggttat ttgtacttat
    27661 tttgtttcat atgaaagtat tgcaccaatt gtcaaattca tttggctgtg cgctgttatc
    27721 ttccaagtga tccttgtctt ttatttcaat tatcaccatg ttatcaaagc tgatctttca
    27781 attgaagcaa tttatccaag ctggtttatc atttacgttg gatttggggt catcactgta
    27841 actgccggga acttcttccc acttattgga aaaattttct tttggatttc attgatctgc
    27901 tatgcgttct tattgccgat catcatcaaa cgtgtctttt tcgtaagaaa tatggctcat
    27961 ccgacacgtc cgctgattac gatcgttgca gcacctggtt ctctttgcct aacaggttat
    28021 cttaaaaact ttgctgaacc taatttttat cttgtcgtag cattgttcgc tttatctcaa
    28081 gtattgtatt tcgttgtgct gtctatgctt ccacgtttgt tcaagcttcg tttttatcca
    28141 agctatgcag cctttacatt cccgcttgtt atctctgcaa cagctatatt tactactgtt
    28201 cattacttca acagtttagg tatctatagt agcgtattgg atttcttaaa agttgcggaa
    28261 tgtctgtttg caagtgtagt tgtcttctac gttcttgctc attacgtacg tttcttaatt
    28321 aaagaacata gcaaacatgg aaaatttgtt tctaaaaaaa tcgcttaata atcgattcaa
    28381 taggttgtga aaaagctgtt ctgcatcaag taataagaac ggccaaacaa aaacagctgt
    28441 tcatgttttt gtactggttg ggcttattac cgcagatgca agcttttgaa caccgtttac
    28501 caagaggtcg taaaagaagt ggtcagcttc aagaaataag aaggaattta cgaaaattgc
    28561 ttctcaaatt tttgtgaaat ttcggcttat ttccgtagaa gctacttctg gaacaccgtt
    28621 tattcgaaac aagaggtcgt acaagacttt tgtcacgacc tcttgttctt ttgtcaaaat
    28681 actggtttag tgctactatg ttcgagtatg cttttagtat ttattgacga aaggaaccga
    28741 tcaaatgaaa tcattgtcac atccaataaa atctgtaaag gattcagcgg tccgcgaaat
    28801 cttacccggt ttactgcttt cttttgccat cgctgccttc agcaggcttc ttgctattta
    28861 tctgccacag ttaggtggag cgactattgc tattttattg ggtattttat taggaaatac
    28921 tttttttaaa cagcctgttc tagcaatcgg cacaaaattt gctgaaagca aactattaga
    28981 attctctgtt tttttactag gtgcaactgt cacagctcaa acgattgctc aaattggtgt
    29041 aaaaggaatt gtttttgtgt tactgcaaat gtcagtcact atcatcggtg cttacttgat
    29101 tggtaaaaaa ctgcaattca gcgatacttc ttctttgatg atggctggag gaaacgctgt
    29161 ttgtggttct tctgctattg cttcaatcgc cccttccatc catgcgaaag aggaagaaaa
    29221 aggacagatc atcacactag tcaatctact gggtactgtg atgatgttga ctctcccttt
    29281 tcttggatta cagttgtttg gtggaaatct cattgaaaaa agtgcacttt tgggtggtac
    29341 actccaatct gttggtcaag tagtcgctgg ggcaagctta cttgacaatt ctgttgtcga
    29401 attcgccatg ctatttaaga ttctacgtat tatgatgctc gttgttgttg tctatctgtt
    29461 tgaaaaattc atgcacaaga aacaaccagc tctgtttgaa acgccaactg atacgcagaa
    29521 aaaacgaaaa ttaccgcttc cttggtatgt attagggttt gtgatctttt gtttggtcaa
    29581 cagcttgttt tctttacctg tagttattga ccaatcggca cattttatca gcagctggtt
    29641 tgagatcact gctttagcgg caattggtct gcgtttagac tttgcaaaat tcatgaaaga
    29701 aggtacacgc ttcttatctt atggattagc tgtcgggctt ctccaaacag tgatggcttt
    29761 actatttatt tggctgatca aagtttagaa tgaacatgaa aaaacagttg tgattctcgc
    29821 atggaacaat gtccgaatca caactgtttt taattttgca gatatccttc taacaacaac
    29881 aattggcgtt tgatttcaac cccatctatt gcgtttccca tatatcctgt cagttttcca
    29941 ttttgcccga tcactcgatg acaaggaata acgattggca atggattggt tcgatttgcc
    30001 tgcccaatcg ctcgcacagc tttcggacta tcaacagcaa cggctacttc tttatacgtt
    30061 cggatttctc cataaggaat ggtcgacaaa gctttccaga cattttgctg aaaaggcgta
    30121 cctctcttta tagataaagg aacatcaaat tcttttcttt ttcccgcaaa atattcttca
    30181 aactgttctt gtgtgcgcgt taaaattcgc tgattcgctg attcgctttc tgaaattggc
    30241 tgaaaagata cagcagtcaa tccggattca tctgcatcta accataaaaa gccgataggt
    30301 gtgtgtattt tcattttgtt tgctcctagt caatagactg gaacttcctc ctttttctct
    30361 atccaaaact acttgccaac taataaaaat tgttcttaaa agtttgataa accgaggaaa
    30421 catggtaaac taaatacgtt agtttacaaa aagaatacct gttcttttac gtaaaatcaa
    30481 attttaaagg tgttatttaa ggtttattaa attttgaatg taaaccatat ataaacatac
    30541 gattgaaagg atagaaacga tgaaagtatc aattgttgtc ccatgtttta atgaagaaaa
    30601 aactgttcct cttttttttg atgaggttga aaaattcaaa ggtgcccatg agttcgaata
    30661 tttgtttatt gacgacggat caaaagacgg tacgttagca gcaatcaaga agctagcctc
    30721 agcttatcct tatgtccgct ttgtctcatt ttccagaaat tttgggaaag aagcggctct
    30781 gtacgctgga ttgcaagctt ctacaggaga tttagtgacc gtgatggatg ttgatctgca
    30841 agatccgccc gaactgcttc ctgagatgat caaccggatc actacttctg atatcgattg
    30901 tgtcggcacc cggcgttcga ctcgtgaagg cgagcctgcg atacgcagtt tctttgcgaa
    30961 aaaattctac cagttgatca ataaaatcag cgatacagaa atggtcgatg gcgctcgtga
    31021 tttccgcttg atgactcgtc aaatggtgga tgccgtccta gaattaacag aatacaaccg
    31081 attttcaaaa ggattattta gctgggtcgg atttaaaaca gaatatattt cttatgaaaa
    31141 ccgcgaacgt gtggctggcg aaacatcttg gtccttctgg aaattattca actactcgat
    31201 tgatggtatc gtgaacttct cggatgcacc gttaacgatt gcttcattta tcggcgcttt
    31261 gtcttgtgtc ggttctggta ttgcaatttt gttcattatt ttgcgggcat tgttgtttgg
    31321 tgatcctaca tcaggatggc cttccatggt ctcgatcttc ctgttcattg gtggaatcca
    31381 attgttatgt cttggaatca tcgggaaata tattgggaaa atcttcttag aaacgaaaca
    31441 gcgtcctgtt tatatcataa gagaaacaga aaaagtgaag ccaaatgaat tgaagcctca
    31501 ccaataatat acgtcaaatg aagtaaacat ttttttaaat gaaaaaggac tgtgacaaaa
    31561 gtcttgtcac agtccttttt tccgaataaa tggtgttcca gaagtagctt cttcggaaat
    31621 aagccgaaat ttcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
    31681 agctgaccac ttctatcaca acctctttca tatcttatta cttcttcatt gctgctttta
    31741 gtgctgctgc taatgggctt tcttctggtt cttctgcttg tgcatatttt ttcagcaagc
    31801 gtcgttcttc atgtttggtc attttcttct tctgctcttt tttatcaagc aatttttctg
    31861 tcgttccatc gtatttacaa cggaaataag caccgttttt accttcgatg atttccattt
    31921 tccggtgaca ttgtgggcat cgatgattag agacttttgg atcttttctt ctgcggtagt
    31981 tgcaatctga attggtgcag acatagattt tgccgtcacg tgtgtttttc tcgcgcagat
    32041 tggccccaca ttctgggcat ttcttttggg taatcgagaa atcttgatat tcttgtttgc
    32101 ttgatttgat ctcattcacc aagcgttttg tttctttttc gattccattc aagaaggttc
    32161 ctgcttgttt ctttcctgca gcaatagctt caaggtcttt ttcccagctt tctgtcaaat
    32221 ctggtgttac taaagaggga tttaccaata ctaataattg cttaccttta ggagatactt
    32281 gcagtccttg aggggtacgt tccatcagtt cagatttgat tagtttctcg ataatctctg
    32341 cacgcgtagc tggggttccc agactgtatt tttccatttg accaagcaag cttgcttctg
    32401 acaatggttt aggcggtgca gatagttctt ttttgatggc gaaatcggga ttgattctca
    32461 ttcctttttc ccatttgact gtttccgtct cagtttcagt ttcgcctttc catcctgctt
    32521 ggacgacacg attttgccga aaaatgaatc gttctttacc aaattcaacc gtaacttttg
    32581 tttggctgac tttatggggt tgagcaaaca gtcctaaaaa acgagagaca atcatttgat
    32641 agatttttcg ctcttcatta cttaattttt cataacgtgc tctgttttca gttggcaaaa
    32701 gtgcatggtg atcggtcact ttactgtctt ggaatacttt tgtctgttgg acgatggctc
    32761 cgtttttcaa atactgcttg acctcaggag caaagtcgct gactgcttgc aatcgttctt
    32821 tcatcgtact tttcatgtca tttgtcagat atttgctgtc tgtacgcgga taagtaacga
    32881 tcttatgggt ctcatacaaa ctttgtacaa gacttaatgt tttctttgca gaatagccat
    32941 agcgttgatt ggcttctctt tggatctctg ttaaatcata aggcaatggc gcagattccg
    33001 tttttaattt ttcttggact tctgtgacag ttcctttgct tgtactgatc aattgaacaa
    33061 gtttttgtgc ttcttcttgt gtttttaaag caaattgatt tttttgaagc attttggctt
    33121 gttcttttcc agcttgcaac gtgatcgtga aatacgtttg tggacggaac tgctcgattt
    33181 tcttttcttg gtctctcact aatgctagtg tcggtgtctg cacacggcct gctgataaat
    33241 tatccgcgta cttcacagtt aaggctctgg tcacattcaa tcctaccagc cagtccgctt
    33301 ttgcccgggc aagagcagat tcatacaatg catcatattc tttcgcaggt cttagttgct
    33361 tgaatccagt tttgattgct ttatctgttt gagaagagat ccataaccgc ttcactggtt
    33421 tattgaaatg cacatactct aagatccagc gagcaacaag ttccccttct cttccggcat
    33481 ctgtcgcaat gactgcttct gagacatctt ttcgctgagc cagctgcttg attgctttta
    33541 attgatgtcc tgttttagga agtggtttga ttcctagttt tttagggatc attggcaacg
    33601 tgtccatttg ccagctctgc cattccttat tcagatcttc cggcattttc aatcctaata
    33661 aatgaccaag ggcccaagta acgatcacct tgggtccttc ataataattt ttgtgctttt
    33721 gattagcacc aagcaccttg cttaggtctt tcgcaacgct tggtttttcc gctagaatta
    33781 gttgtttcat aaaattcctt tctgctttct actgtgatgg gctgatgatt tttcaacaac
    33841 gcaagtccct tgtattgctg taaatcatcg tcacactctt cacaccagcg ctcatctccg
    33901 tcattccaaa aggcgctgag aaagttactt tttgcattga tatattcata attttgatgg
    33961 atttcgttcc attgtttttc gtacgcttgt cgtgcttctt cgaaagactc gaatgtttct
    34021 tcttcttcaa tatcttcttc ccagtcttca aaaaaccacc aaggctcatt gtccccgaac
    34081 atggtaatta cttgatacat actcgtgctc cttctttcag cttttctaga ctcatcttag
    34141 tcacttttca agaaagttgc aagaattttc tcttataaaa gggtcattta atgaacatct
    34201 taaaattgtt acaaatgaat ctgattgttt aaggcaaagt agcgataaag ttattgatca
    34261 tccaaaaata gctgtcttct ggatcgattg ttggttcttt tgtaaagtat cctaaaaaac
    34321 gtaaggaaag ataaccgtgg atcatactac gaagttccct gcttcgatga actttttctt
    34381 ctttcgttaa ttgaaaagcc tcaaatatct gcaaaatgat ctgattcgtt tcatggattc
    34441 catctaataa aacagcttca ttggtatgcg gtacactgat cagtaactca tacactgctt
    34501 gatttgaaaa agcaaaggat cgctaagttt ctgcatagat ctgcagtgct tcctgaccac
    34561 tttttcccac aagagcccgt cgcagctggt cattaagttc ctgcagaagt ttcgctgtta
    34621 gcgctgtttt gacatcacga atatttttga aatggttata cagcgaggga gccttgatat
    34681 ccaagcgttt ggctaaatgc tgaaaggtta tttgctgaac atccatctct tctgctagtt
    34741 ctcgaaagca gtcgatgatt ttctcttgag acagatttct ttttttcatt tccatgtttc
    34801 ctctcataaa ataaatcctt cactctttca gtttaccacg aaaaagaaag agaaaaaaga
    34861 gactttcatt gataaactac atattgtagt ttataataaa gagctgtaga tgaaaatttt
    34921 cagaaagaag gagtctaatt ggctgcaaga ataaaggaaa aattatttag gaaaagagaa
    34981 acttgggaac aaaacttgtt tgtcttatgg tttggaacat ttatggctgg tatcggattt
    35041 agtttagtca tgccttttat gtcactttac atagatacgt taggtaatta tagtacgtca
    35101 caattgaact tttggagcgg attgactttt tcatctactt tcttggtgac tacgcttatc
    35161 tctccttggt ggggaaaatt agccgaccaa aaaggaagaa aattgatgct gttgcgtgct
    35221 tcattaggaa tggcaatcgt catttctttg atgggttgcg tgactagtgt gtatcaatta
    35281 gtcgcattgc gtctgcttca agggattttt tctggttata tcagtaatgc cactgccctt
    35341 gtcgcaactg ggacgccaaa agaaaaaagt ggtcaagttc ttggaacctt agcaactggt
    35401 tctgttacag gaacattact tggaccgctt cttggcggga tctctgcttc agcgtttggc
    35461 tatcgcccta cctttttcat tacagggatc atcttatttt tcgtcttctt gttgagtctg
    35521 tttttcgtcc atgaaaaatt tgtcccagtg aaaaaagaaa acatggtttc tgccaaacag
    35581 attttcaaag acttgaaata tccacacgtc gtcatcggta tgttcgtcac aacgatgatc
    35641 atccaagcat ccaacaattc cattagtcca atcatcagtc tgtacattcg ccaattgctg
    35701 catggacatg ggaatgttac gttgatcagc ggaatcattg cttcgatacc tgggattgcc
    35761 actttgatag ccgctccaag atttggtcgc ttaggtgaca agattgggag tgaaagaata
    35821 cttgcgattg gtttgggttt tgcgattctg gtttatatcc caatggcatt cgtcacgaat
    35881 gtttggcaat tagctgcctt acgttttttg attggtatct ctgatgcttg tctgctccct
    35941 gctgtccaag cattgatcac aaaatattcg cctcatcaag ctgctggacg gatcttcagt
    36001 tataaccaat ctttccaagc aactggtaac gtagtagggc ctatgatcgg ctccagtgtt
    36061 tccagtgcat ttggttatcg cggcgtcttt ctttcgactt ctcttcttgt tttggtcaac
    36121 ttcttgcttg tacgacacaa tacacaagag atccatcagc aagaaactgc agaagaaaaa
    36181 gcttcaggag tctccatagc ttcagggcac tctccttctc atagacaata agcgtaaatg
    36241 aaaaacactg cttgaacagt tgtcacagac tgtccttgca gtgtttttgt ttaggcctgt
    36301 tttttcaaga actcagataa gaggaataat cccttttcca aggtttcttt tttacaacag
    36361 taacctagtc gggcatgctt cggtaaatca aaggcacttc ctggtactaa tagtactcct
    36421 gtttcttcta ataaattgat acaaaatgtc tggtcatctt cttcgatggc taatttaatg
    36481 aaagaagtag agacgtaatt gggagcgaca agttcaactt tcggttcatt ggctacccat
    36541 tgttttaaaa tcacaaggtt ctctgtaatg atctttttgt ttcgcgccaa tattttttct
    36601 ttattcttca atgcatgtac agcaagatca tctgacagga ctccgccgca aatcatcgtg
    36661 tagtctcgat actttcgaaa aacttctgcc aactcaggtc cagtagctgt ccagccaata
    36721 cgaatacctg gaatggagta ggttttggat agggaattgg ttgctatacc tttttcatag
    36781 acatctacga tcgacaagaa ttcaccttta tccgttaaag gcgcatatac ttcatctatc
    36841 aatacatatg catctactgt acgagcaatt tctgcgattt tttctaaagt tttccgatct
    36901 agcaatgtgc ctgtaggatt gtttgcacta ttcaaacaga tgatttttgt ttcaggtttg
    36961 actaaggctt ctagctgttc taaatcaaac tgccaatctt cttcttcctt caaatgaaca
    37021 aagtcaacag cggcacctaa agatttgggg atgtcatata attgctgata agtaggaagc
    37081 atcgaaacaa catggtctcc tgcttccact aacgcgtata gagctaaaag attagcacca
    37141 gtcgctccat tcgtttgtaa gatgttttcc ggatcgacgg tttgataaag agaagcgact
    37201 tcttgtttga aaacatctga tccttcgatc cagccatagt ccaatggttc gtttttgcgt
    37261 ttttcgaaaa atttacttac gctagtccca tctaagccaa cgagttcgtc taatgtcaat
    37321 gcttcgatcg agctttgtgc aatatcatat accgctttct tttcccattt atttagccat
    37381 tcttctacac cgaaatttgc gattttcatg tttctttccc ccttatatct tgatccagcc
    37441 tacagcaagc aacccgattg aaagcagtgc ggccacacaa atcagcccaa tcattacttt
    37501 ttcttttccc gtaatttgat gtttggtttc acgacaagcg atccagtaga agcaaaaacc
    37561 aggaagatag gcaatactac ataaaaggac ataagaaaat cctgctaaaa tgatgcccat
    37621 caattcaaaa actacagcaa tcccaccgat cactacttgt tttttgattc cttcttttaa
    37681 agaaaactgg acttgataga tcccgacaag tagataacaa atcaaaattg ccgctgtaca
    37741 taaagaatag gcaaattgat aggcataatc agtaactaag aaagtcaata agaatagatt
    37801 agtcaagatt gcagtaacaa ctaacgaata agttggggca ttttttttat tcgtttttcc
    37861 ccaacctttc ggcaacagac cgtctcgagc catcaacatc gttgtttctg ctgggagcat
    37921 cgtccaagac aaccaagaac caatgatcga aataatcaag ccgatattga tcagcgctgc
    37981 tccccaattt cccacgattc cttttaaaat atttgccata gcaggttgtg aaatagttgc
    38041 taactcttct tgagataaga cgccgtatgg caagatcgaa gcaaggatat agataaacaa
    38101 taaacaaagc agaccaatga tcgatgcttg ctgggcttct gaacgtttct tcgcgcggtt
    38161 ggctaaaaca cttgcgcctt cgatccctac aaagacccac atcataacca tcaaacaacc
    38221 tttaatctgc aaccagatcg ttccttgctg ccctgttcca ttcactagat tatcagatac
    38281 gttaccccaa aaatcagcag tgaatatatt cactttaaat gcgataaata caaatattaa
    38341 aaataggaat aatggcacta atttacagat cgtcacgatt gtattgatga cgctcgctcc
    38401 ttcaactcca ttgtttacta ataaagtcag accccaaata aaaatacttg caaataaaat
    38461 agaaggaaca ttttgtccac ctttgaatat cgggaaaaat tctcccatgg cgctcatcag
    38521 catcgttgca aaagcaacat ttcccagcca cgcagacaac cagtaacccc aaccactgat
    38581 gaagccacct aatttaccaa atgaagcttc cgcgtaaccg aaaataccac catctagatc
    38641 tggacgcttt tctcctaaat gattcagcga taataccaaa gctaaaatac caatcccaac
    38701 gattatccat gaaaggatcg ctggccctgg agctgcagaa ctagccagat cacttgtgat
    38761 cccaaacaca ccgcttccga ttgacgaact gacaaccaga gcaaccagtc cccatttgct
    38821 aatacccgtc gatttctttt ccatttccat caccctcact tataaaattg aacaaatcac
    38881 ttgcaaaaga ttcccttcta ttttgcaact gctcttgctt aacaaaaagc aactttattc
    38941 atattattgg atattatgaa ctttttacac aagtatttca agtataaaat gaataaaaat
    39001 taattttatt gcaaaaaaac agctatgcta tccaaaagaa aaggtgtttc ttttgttagc
    39061 catagctgtt ttgattcatt taatatcgct taaacgtatt gtaaaacttt tgtttcttga
    39121 tatgcgtggt cgatcaaacc accacccaga cattccattc catcataaaa tacaactgct
    39181 tgtccaggag tgattgcacg tacaggttcg tcgaatacga cttctgcacg attatcatcc
    39241 aataagcgaa ctgtcacagg aacgtcttgc tgacgataac ggaatttcgc tgtacacttg
    39301 aattcttttg gcattggttc attcgtcgta aagtggattt cacttgcatc caagcttgtg
    39361 gcatacaatg caggatgatg gaacccttgc cccacatata atgtgtttgt cgctagatct
    39421 tttcctacaa caaaccaggg ttcttgcgaa tctcctccgc caccgatccc tagtccttga
    39481 cgttgaccaa tcgtataata catcaagcca gcatgttgtc ctttgacttc accatcaaga
    39541 gtgaccatat ttcctttttt tgctggcaga tagttgctca aaaattgttt gaagttcttt
    39601 tcaccaataa agcagattcc cgttgaatct tttttcttag cagtcgcaag gcctgcgcgt
    39661 tcagcaattg cccgtacttc tgatttttcc atgccgccta gagggaacat cgtttttgaa
    39721 agctgttctt gtgataattg gctaaggaag taagtttgat ctttattatt gtctactcca
    39781 cgaagcatgt ggaccacgcc gttttcatcg cgagtgactt gcgcataatg tccagtagct
    39841 acataatcgg cgccaagatc cattgcataa tccaagaatg ctttgaattt gatttctttg
    39901 ttgcacatca cgtctgggtt aggtgtacgt cctgcgcggt attcggctag gaaatattca
    39961 aatacacgat cccaatattc tttttcaaaa ttcacagagt aataaggaat cccgatttga
    40021 gcagctactt tcgctacatc cttataatct tccgtggccg tgcatacacc gttttcgtcc
    40081 gtgtcatccc aatttttcat gaatattccg atcacgtcgt atccttgttc ctttaacaaa
    40141 agtgctgtta cagatgagtc gacaccgccg ctcatcccta cgacaacgcg tgttttgctg
    40201 ttgtctgtca tgatatcacc atcatttcaa ttagattctg aaacatctac gagttgaagg
    40261 tcgcaacttt tcagtatttt acattatacg tcatatttct gaaaatttca tcccttaatt
    40321 atcaagtcgt aaaaatactg ttctactgga cctattcagt acacaaacat gatagtcaac
    40381 caaaaaatca ctcatctttt gagcattcga tataaaaaat tggcatactc ataaaaaggg
    40441 ccagcctttc ggcaataagg aataatttta taaattgtga aaaactattt ttcaaaattg
    40501 cttcttatcg cttaaagctg cacgtctcat ttacaacctt ttctttttag atgttatgag
    40561 ttgctcagat aagtgatttt gtttactcta agaaaaaagg gaggcaaaag atacatgaat
    40621 tactttatca gtgatatgca ctttttccat gaacgcttat taggaaaaaa cgactttgct
    40681 ccgcggctct ttgcaaatgt ggatgaaatg aaccaacagt tgatcactag ctggaatgca
    40741 gtaataaaag aaaccgacca tgtttatcat ctaggcgacc ttgctatgca tccgcaatat
    40801 gaaaaagcaa atgcagaaat tttggaactg gtcactcaac tgcaaggaac aatccacttg
    40861 atcaaaggga accatgacag tcgcgctttt ttccgttatt tagaaaaaaa tgatcctagg
    40921 cttactgacg gaagtcccaa attttatttt tatgatgtag gtgccatcgt taaattcgac
    40981 catcaacaat attacttgac tcattatccg atgttgctcg ggcctaacga cactatccgc
    41041 aatcttcatg gacatatcca tcattacagc gtacctatcg agaacgatgt caatgtaggc
    41101 gtagacgcac cggaaagaga actcttaaag gaaaaactac catttggcac tccgttatct
    41161 gagcaacaaa tcaaagaaat ctatgaagca aagaggaagt acgataggtc gtgaaaaaac
    41221 tgccctatat caagtattaa acaaataaag ataattactc atatttttca tttgagtagg
    41281 cttattgcca caggaacatt aggatcagaa atggagttgt aacaagactt ctgtcacaac
    41341 tccattccat actattcgtc tttttgctca tggtgttctt ttaccagttt tttctgtaat
    41401 tctttatcat aatttcgggc agctgacgtt aaaaataaaa cgattccgca taataaaatc
    41461 ccgattccgg aaaacaagaa cattttttct acccctaaac gatcagctaa tggtcctgca
    41521 aataataatc caattggacc tgccaggttc atcaaagagt tgaagttccc taagacacga
    41581 cctaaaacat tcgattcata actctgctgg atcattgcca tcagcagtgt attgaaatat
    41641 ggcgttgcac aacctgctcc tgcattcaag ataagaaagt acaagaaccc ttgactatcg
    41701 tttggaacca atccgcttgc accgatcgtc aggcccataa tgatgtaggc tataataata
    41761 ggtttcattc ggtctttcca tgttcctata aaactgatca gtgcaccgcc taagagcatt
    41821 ccgactgcgt agactacttc cactgctccc gcctgtccaa cggaaccacc aaagtaatct
    41881 agcgtcatca atggatacag actgattgct ggcatgaaca acagcatcac aaatgcacca
    41941 ttgatcgtta tgtaccataa ccctttatta tccatcaatt gctgcagccc aaattttgca
    42001 tctttaaaaa tagtaagcgg aacttccaat atttcaggag aaacttttgg gatcttcaca
    42061 aaaatcaata agccgacacc aaaaactgcc cccaatacat ctaataaaat aaggtaattg
    42121 acaggaacga ctgaaaacaa agccgcacct agagctggcg caatgatgaa attcgctgat
    42181 tggaccattc ctaactgtcc attcgtcctt gttatgtgag aagatggaac gattgtcggc
    42241 ataatcgact ggatcgttgg catttggaat gtttgtgcga ccgaacgcat aaacaaagac
    42301 acaaaaacta gccaaatcgg gaaggattct gaaatcgttc cgactactgc taaaataagc
    42361 gcgaaaatcg ctacaataat atccgtgata atcaaaagag cttttttatc ccagcgatcc
    42421 actagcggtc ctacaaatgg gcttagtaaa accatgggaa tcattcctag taaagtagca
    42481 aaactcaaaa ttgtcgccga accggtttct cttgtcaaat accagatgat cgcatattgg
    42541 acgaccatac tagtgattcc tgacaaaaac tgtcccgata aaaagaacaa ataatttctc
    42601 ttccaattcg ttaaaacgac cgactgttca ttttccataa acatccctct tttctttttc
    42661 attttgtata gcgtttgcac aacaatcatt atacgggatt cttttttcaa aaaatacctt
    42721 tttccctaaa taacaatcgt ttcaatttaa atttctttac ttagaaacaa taaatcagca
    42781 aaatattaaa aataacaaca tgtgttaaat ttaaaaataa aaaagacaac taatttgcgt
    42841 cgtccttaca a
//
LOCUS       DASHRQ010000018        42390 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.018, whole genome shotgun sequence.
ACCESSION   DASHRQ010000018 DASHRQ010000000
VERSION     DASHRQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.018"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(154..552)
                     /locus_tag="U2M88_001087"
     CDS             complement(154..552)
                     /locus_tag="U2M88_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEM8373481.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(549..1322)
                     /locus_tag="U2M88_001088"
     CDS             complement(549..1322)
                     /locus_tag="U2M88_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEM8373482.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1319..2002)
                     /locus_tag="U2M88_001089"
     CDS             complement(1319..2002)
                     /locus_tag="U2M88_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEM8373483.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2169..3056)
                     /locus_tag="U2M88_001090"
     CDS             complement(2169..3056)
                     /locus_tag="U2M88_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM8373484.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3053..3922)
                     /locus_tag="U2M88_001091"
     CDS             complement(3053..3922)
                     /locus_tag="U2M88_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8373485.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4059..5345)
                     /locus_tag="U2M88_001092"
     CDS             complement(4059..5345)
                     /locus_tag="U2M88_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM8373486.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5618..6196
                     /locus_tag="U2M88_001093"
     CDS             5618..6196
                     /locus_tag="U2M88_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEM8373487.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6196..7125
                     /locus_tag="U2M88_001094"
     CDS             6196..7125
                     /locus_tag="U2M88_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8373488.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7122..8858
                     /locus_tag="U2M88_001095"
     CDS             7122..8858
                     /locus_tag="U2M88_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEM8373489.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8921..9757)
                     /gene="pheA"
                     /locus_tag="U2M88_001096"
     CDS             complement(8921..9757)
                     /gene="pheA"
                     /locus_tag="U2M88_001096"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEM8373490.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9754..10263)
                     /locus_tag="U2M88_001097"
     CDS             complement(9754..10263)
                     /locus_tag="U2M88_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEM8373491.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10256..11551)
                     /gene="aroA"
                     /locus_tag="U2M88_001098"
     CDS             complement(10256..11551)
                     /gene="aroA"
                     /locus_tag="U2M88_001098"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEM8373492.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11564..12655)
                     /locus_tag="U2M88_001099"
     CDS             complement(11564..12655)
                     /locus_tag="U2M88_001099"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEM8373493.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12672..13838)
                     /gene="aroC"
                     /locus_tag="U2M88_001100"
     CDS             complement(12672..13838)
                     /gene="aroC"
                     /locus_tag="U2M88_001100"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEM8373494.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13840..14904)
                     /gene="aroB"
                     /locus_tag="U2M88_001101"
     CDS             complement(13840..14904)
                     /gene="aroB"
                     /locus_tag="U2M88_001101"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEM8373495.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14937..15959)
                     /gene="aroF"
                     /locus_tag="U2M88_001102"
     CDS             complement(14937..15959)
                     /gene="aroF"
                     /locus_tag="U2M88_001102"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEM8373496.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16618..17592)
                     /locus_tag="U2M88_001103"
     CDS             complement(16618..17592)
                     /locus_tag="U2M88_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEM8373497.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGPGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17573..19456)
                     /locus_tag="U2M88_001104"
     CDS             complement(17573..19456)
                     /locus_tag="U2M88_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEM8373498.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19474..21699)
                     /locus_tag="U2M88_001105"
     CDS             complement(19474..21699)
                     /locus_tag="U2M88_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEM8373499.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21814..22848
                     /locus_tag="U2M88_001106"
     CDS             21814..22848
                     /locus_tag="U2M88_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8373500.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRTYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22915..23928)
                     /locus_tag="U2M88_001107"
     CDS             complement(22915..23928)
                     /locus_tag="U2M88_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM8373501.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKHLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24006..24194)
                     /locus_tag="U2M88_001108"
     CDS             complement(24006..24194)
                     /locus_tag="U2M88_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEM8373502.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(24634..24762)
                     /locus_tag="U2M88_001109"
     CDS             complement(24634..24762)
                     /locus_tag="U2M88_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEM8373503.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(24765..26138)
                     /gene="rlmD"
                     /locus_tag="U2M88_001110"
     CDS             complement(24765..26138)
                     /gene="rlmD"
                     /locus_tag="U2M88_001110"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEM8373504.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26249..27304)
                     /locus_tag="U2M88_001111"
     CDS             complement(26249..27304)
                     /locus_tag="U2M88_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEM8373505.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27314..28744)
                     /gene="gatB"
                     /locus_tag="U2M88_001112"
     CDS             complement(27314..28744)
                     /gene="gatB"
                     /locus_tag="U2M88_001112"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEM8373506.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(28741..30213)
                     /gene="gatA"
                     /locus_tag="U2M88_001113"
     CDS             complement(28741..30213)
                     /gene="gatA"
                     /locus_tag="U2M88_001113"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEM8373507.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30213..30518)
                     /gene="gatC"
                     /locus_tag="U2M88_001114"
     CDS             complement(30213..30518)
                     /gene="gatC"
                     /locus_tag="U2M88_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEM8373508.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(30624..32660)
                     /gene="ligA"
                     /locus_tag="U2M88_001115"
     CDS             complement(30624..32660)
                     /gene="ligA"
                     /locus_tag="U2M88_001115"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEM8373509.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(32677..34911)
                     /gene="pcrA"
                     /locus_tag="U2M88_001116"
     CDS             complement(32677..34911)
                     /gene="pcrA"
                     /locus_tag="U2M88_001116"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEM8373510.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35066..36625
                     /locus_tag="U2M88_001117"
     CDS             35066..36625
                     /locus_tag="U2M88_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEM8373511.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTSESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(36736..38667)
                     /locus_tag="U2M88_001118"
     CDS             complement(36736..38667)
                     /locus_tag="U2M88_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM8373512.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(38685..39599)
                     /gene="pfkB"
                     /locus_tag="U2M88_001119"
     CDS             complement(38685..39599)
                     /gene="pfkB"
                     /locus_tag="U2M88_001119"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEM8373513.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSETETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(39596..40348)
                     /locus_tag="U2M88_001120"
     CDS             complement(39596..40348)
                     /locus_tag="U2M88_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEM8373514.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(40507..40716)
                     /locus_tag="U2M88_001121"
     CDS             complement(40507..40716)
                     /locus_tag="U2M88_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373515.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(40727..42055)
                     /locus_tag="U2M88_001122"
     CDS             complement(40727..42055)
                     /locus_tag="U2M88_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373516.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            42191..42346
                     /locus_tag="U2M88_001123"
     CDS             42191..42346
                     /locus_tag="U2M88_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373517.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
BASE COUNT        11783 a         9177 c         7617 g        13813 t
ORIGIN      
        1 tagcatattt tgaaaagaaa gtctgatgct tagagtagaa tttcataaaa atttactatt
       61 tcataagtat tcacgagaaa aatacaaaca aaaaagcgga aataacgctc atccgccttc
      121 ttgtttgtgt ttatttcctc ttcaaacgct tgtctatgat tcagactttt ctctatcgtt
      181 attttcatct aaaagttctt gcattccgat aatttctcga tattgatgaa tcttagtgat
      241 accaatgact ttgtctacat caatactaaa acaaatacca ttacctggaa tatcgatgtt
      301 gatgccttga ccaatcgcat ccattacttt attcgtcaaa ctatcgggta ctaatgtcaa
      361 cacaatatct ttttctggcg aaatgtccaa acctaaaaat ttccctgaat catgtacacc
      421 agaacctctt ccatgtaaga tcgttccgcc tgctgcacct gcttcttttg aaaagtcgat
      481 aacatcttcg cccattcctc cgtcaacgat cgtcacaatc atgtgaagat tcaatgctac
      541 aagatctttc atttgctttc ccccaatttt tgcaatcttt ctttttcgat tctgtctttt
      601 tcatcttttc gtttataaat atatcctagt actaaagtgg ataatattgg tgtcaatgca
      661 atcattgcaa ccacaccaaa cccgtcaatg atcggatctc ttccttcaat ttgtgcagcg
      721 attgccacgc tcattgataa aataaatgtc gaacacatcg ggcccgttgc tactccaccg
      781 ttatcaaaag ccattgctaa aaagatcttg tccacttttc gtcctaaaat aaatgctagc
      841 ccgtatcctg ggatcaagaa ataataaaga gagaatccga cgagcaaacg ccacatagac
      901 aatcctactg ctgcgccgat cccaattgaa attaccatca gcatgatttt cgatcggatt
      961 ctcccctcgc tcaattcttc aacctgtttg atcattacat gaatcgccgg ctcagcaaac
     1021 cctactaaga accccattaa aaagccaagg ggaaccagta tataattgta aggcaatccc
     1081 gcaatagcac tgcctaaatg ctgtcctacc ggtacataag ccatattgac tccgtgcagg
     1141 aaaaggatca acccaatagt cgttatcaag aaacctttca taatgtcaaa gaaacgcttt
     1201 ttgcgcatac gaaaactaat gatattcatt aaaacaaata ggattacgat aggtgccact
     1261 gccaaaatga cttccatggt aacgatttcg attccatcta tcacttgctg ccagaaactc
     1321 attggaagat cacccccaaa atcatcacgg caagaatcgg ccctaacgaa gcaataccga
     1381 ccattccaaa actgtcattt tcatttttct tttgtttgat catactagtc atccctccgg
     1441 caagggccaa gataaatgga acagttactg gaccagtagt cactcccccc gaatcaaaag
     1501 caatcggcat aaattcggaa ctggtgaaaa atgaggcgat caaaacgcct atgtagccta
     1561 tagccatcaa ttgataatag gatagcttga atacgacccg aagcagcgca aaggctagaa
     1621 atactccagt gccgacgctg acgatcccaa tcagcaacac tctgccaatc tctccgtcag
     1681 atatatcata tacttgttgt cccaaaactt gcacggaagg ttcagcaatc gtgatcacga
     1741 tcccgatagc aaatcccaaa ctgaccaaaa cggtcaaact tttctttttg atcatatatt
     1801 tccctactag atctccgact tccatcatag aataatctgc tccaaacaaa aagagcgtca
     1861 ttccgatcat catgattgcc gcaccaacca gaaatgaagc gaggtcttct ccttctaacg
     1921 gtgcaaaaac aaacgttaaa atgacaatca ggacagtcat cggcaatata gcagcaatca
     1981 cttctttaaa attttttagc atgtatcctc ctcctttact ctttaagttt accttaaaat
     2041 gaaagagttt tcgagcacaa acttaaaacg ttatcattta atttttctca acataaaata
     2101 acacacgtcc caaaagtatc tttttacttg gaaaaatgac tttgaaggaa cgcgcttatt
     2161 gtgttttttt attttacaga gccgatcatt ccttgaacga attgcttttg caaaacaaaa
     2221 aatacgatgg cagttggtaa agtagcgatg actaatccag ccatgatcat tccatagtct
     2281 ggtgtatagg aagcacccat agcagataaa acaagcggaa ccgtccgttt atcaggagat
     2341 tgcaaagcta ctaagggcca taaataattg ttccagctac tcatgaatgt gatgattgca
     2401 gccgcggcat aggtattttt agccgtcggc atatacactc taaaaaaaat acttagttct
     2461 tttagaccgt ctaatcgagc ggcttctact agatcttttg gaaaagattt aacattttgt
     2521 cgaaagaaga agatcaaaaa agcagtgcta acagatggca ggatgacaac caagaaactg
     2581 ttgattccca aaaccgttcc gttcaattgt gaaaacattc gatataaagg gatcatcaat
     2641 gccgcaaaag gaaccatcat cgatagcagt agaatattga aaattaggtc ttttcgttta
     2701 ctttgataaa tctcaaatcc atatcctgcc attgacgaaa tcagcaatgc aaacacagtg
     2761 gtcaccaaag cgatcatcgc tgaattccaa agggaacgag taaaattcag ctcattggaa
     2821 aacaaatttt gaaaattgat gatcaagtta ctgccaatct ttatttttcc cgatgtgata
     2881 tccacgggtg tattcgtcat tcccaaaacc atccagacaa aagggaaaat ggaaataaat
     2941 gataggatgc ttaatgctgt atactttaat actgacccaa ttgtcgaacg aagttctaat
     3001 gcttgttttt tgacgttggg gcgttcgatt gtttcttttt ctaccatatt tttcatcgac
     3061 tttctcctcc tactttcgtt tggattatcg acaatatcac aataaaggaa acaataacaa
     3121 aggaaactgc tgccgcatat ccaaaattag gcgtgaattt ataactcaaa ttatagatgt
     3181 attgagatag ggtggtcgtt gcatttgctg gtcccccgcc agtaatattt tgtacttcat
     3241 caaaaagctg caatgtcccg atcgtcgaag taatggaagt aaacaaaatg attggtttta
     3301 aatttgggat cgtcactttg ataaactgct gccatttaga agcaccgtca atagatgctg
     3361 cttcataaat ctccgaatca atattttgca tgcctgaaag gaaaaaaatc atattgtatc
     3421 cagtccaacg ccaagtgatc gaaagaatga tcagaactct cgcccaaatt ggatgagtca
     3481 accagccaat cggtgtgtca atcataccaa tgcccaacaa caagttgttt acaaggccag
     3541 attgagaaaa tagacttttc atgatcagag aataactgac catcgaagtg atacatggaa
     3601 gaaagatagc ggttcgaaac aaccctttat atttcaactt tttatcattt aaaaggttgg
     3661 aaataaaaag tgctagaaac aacatgatcg gtacttgtac aagtaaatac aggaatgtat
     3721 taaaaaaagc ttttttaaaa gttgcatctg tcaacatccg ctgataatta cctaacccgt
     3781 taaatgtcat attgacaggg ggaccacttt gaaaagacgt gatcaaagcc aaaaccatcg
     3841 gaataaaaac catgattgat atcaaaatga ctggcaagat taaaaaagca tgcccgtttt
     3901 tgaataaatc tttctgcttc attcttttcc ctccaattct atcattgatt atctacatga
     3961 aaagacaaga aagacaatat ctgtaaggca gacgtgatct tttttgtctt ttcgtttgtc
     4021 tgatgaaaaa gcgaagaaat tcactttttt accaccgatc aattagccaa ttgtgcttct
     4081 acttgttttt gtgtatctgc tagtaccttg tctgtctctt ctccattgat gattcgatga
     4141 agagcttcag ctacgactgc ttcaattgca taggtctcat gtccgtaatt cacttctggt
     4201 atttcagatg tccatgttga aaaatcgtca aagatctttt gaccactgta aaattcagaa
     4261 gcttcttgat aagcttcttg ttcattcgcg ctaagcatcg tagatactag tccgatttct
     4321 ttcgcaagag tgcccatcag ttcttcattc gaagcaaacg ttgcttctag gaaatctttc
     4381 gctgaatctt ctccagctac tcctttgatc acataccagc cagcgccgcc aaggttagaa
     4441 gcttgcgctt tttgcagatt ttctggaagt gcaggtattg gtgcaatctt ccattttcca
     4501 gattggtttt ctgctccttg gatcgttgaa gaataccaag cgccagttgg gctagaagct
     4561 actgctcctg attgtatcgc agttactcca gcattccagt cagaggtttg ttcgactaga
     4621 tcttctttca aaagagtggc aaacaattca agaccatact ttaaagatgc attgtcttgg
     4681 atcgtgactg tctccccatc ttcagatgta taccattctc ctgcctgttg cataatcatc
     4741 cttactcgac caaggtcaga aggattgact tcagcgattt tctttcccgt cttttctttg
     4801 acatctcgcg ctacttgaat atactcgtcc cacttcagat tctccatatc ctcttctgta
     4861 taacctgctt ctgccatcag atccgtacga tagaagtttg ctgttacgcc cgaatcgaaa
     4921 ggaacaccgt atattttatc tcctactttg tttaccgcaa atttatacgg tgcaaaatga
     4981 tcttccttta caatagcaga caaattagcg aatgcatctg gataagaagt caaatagcct
     5041 tgtatcctat aatcttcaat caagacgata ttgggcaatc cttctgtatt cccgctcgct
     5101 aaagcggtgt tcagtttctg aacgatatca tcctgagaca tcgtaacaac ttctatctct
     5161 gtttcttcgt tctcataaac tttttttgct tcattcactg ctttgatatt gaatgtctca
     5221 tcccacgccc agacagtgac tttttcttct ttgtctgctg tactactagc acttccgcat
     5281 cctgttaaga aacctgatgc tgccgctaaa caaacgactc cagcaattaa ctttctgatg
     5341 ttcattttta tccgctcctt ttccatccat tagttgttta cgtttgctac attcatcata
     5401 ccaaaatttg aaaacggttt ctttattatt cagatacttc aaaaatttct cctaaaataa
     5461 taaaaaaata caaatcaact atgacaaaag gaataatctt agcatctaga ccaaagatcg
     5521 aaatggtgag gtttctttaa aataaaaata tccttgcatt ttctcctaga aataagtatt
     5581 tttaaattac aagactattc ttaaggcggg taaaaaaatg ccaatacgcg taattcttat
     5641 cgatgatgaa cagacgattt tagatggaat gaaacagttg atcaattggt ctgagcacgg
     5701 gtttcaatta atagggactt tttccagtcc gctgaaagcg cttgattttt ctttggtaca
     5761 tgcaatcgat atcgtggtca cagatgtaat gatgcctgaa ttaaatggaa tcgaattatc
     5821 gcggctatta aaacagcaac aacctgaaac acagatcctt atcctctcta gctatgatga
     5881 atttgatatg gttaaagatt cttttaaaga aggcgtcgct gattatcttt taaagcctcg
     5941 attgagtcct gatttttttc taaattcctt aactgccgtt tcacaaaaaa tcaaaaaaaa
     6001 gcccaacaaa agcccaatca ccaatcgtta tgagcagatc tgcgagaatc tttcacagca
     6061 gattgctgga caaactcgtc cattgcttga ccctcttctt tttgaacaca ccttgttttt
     6121 ctgttatata ccgaaaaacg gccattcagt tcaaaacaag gctctaaaca aaaacagtcg
     6181 atcacccatt cattgatgga tagcgacgat tcgtttacta tttatcctgt ttctaccttt
     6241 tcggaagaat ccggctatgt aatcaatact gattccaaag atacacttag gaagtttgtt
     6301 caaaagtttc gtcaagaagt ttttccaggt gatttttttg ttgtttctga ttcttttgca
     6361 atccaagata tcaatgaaat attccaagag atcagaaatt gcaccaaagg acaacgtttc
     6421 tatcataagg gaaagaacct tgcttatcag catgaacttc ttccgttatt aaccaaccac
     6481 gagcagcaag caagtagtta tttaacaaaa atcgttcata aagactacgc tttagctgtc
     6541 aaagaacttg agaatttctt cacgacgata ttggaacagc ctattgatcc agcttatttg
     6601 aaacatgagg caatcaatcg tttctatgca ttactcaacg ctttggctga cgagcatgtt
     6661 gggaatgaag aactttcttt gctcaagatc gcccttcctt ctttattagc cgatgctgag
     6721 tatttcgaag atttttctat tcttgtcaaa gaaacacttg agaaactcac ccacttttct
     6781 gcaaatgcaa aaaaagacaa tttcgatatc ttgactttga tctacgagtt cgtccaggaa
     6841 aattatgaga aagaaatcaa tttacaactt ttatccgaaa aatatcattt ttcatatagc
     6901 tatctctcag ccatctttac tgaaaaattt ggtatcaact tttcaaaata tctaaaaaaa
     6961 gtccgtatca gtaaagccaa gctctttttg acggaaacac ggcttacttt gacggaaatc
     7021 tgtgagaaag tcggctatac cgaactgggt tatttctcca gagtttttaa agaagagaca
     7081 ggaatgacac cttctcaata ccgtaaagga acttttgtaa aatgagaaaa aaaatcaaag
     7141 agcatgaact ctttgccaaa cttctgttca ttttgttcat cggtcttttt ctgcaagcca
     7201 tcgtcatttc tttgtttatt tatcatcgat caagggatgc ctatatccag ctgttcaatc
     7261 aatcgaatga tgtggtacta aaaaagattc aatctgaatt tgaaagtttg aatgatacaa
     7321 tcgagaatac attagccgct tttgattcta atccagctgt caaaagctat ttttccaatg
     7381 atccagccca acatatggaa cagcttcagc aactaagaac aatccaaaaa atgaatgact
     7441 ctctttcaaa aatccatcca atgatcgatt acgatgtatt gatctttgga gaaaatggaa
     7501 gaacttttgt cggaaacgac atgctcactg ccgtatctgc cgattctttt tttcaatctg
     7561 cgattgctca gagagtgaat gaacgtgctg ctgatacaca aatgcttttt gcacaccacg
     7621 gattgactct tcgcgataag aaagcaccca gcgtcttctt tgtcaaaaaa ctgaaaaata
     7681 cattgaatca cgtttacgga tatgctgttt tatcgatctc atccaagcaa ctagcgaatc
     7741 tttttcaatc tgtagtaaat cctgagatat cgaagatctc cttcatgaac gaagaacaga
     7801 cgatcatcgc atcaaatgaa gaaaatacaa ttgggagccg atcaatgatt ttcaaaaaac
     7861 taacagtagg tgaaacaacg atttcaaatg gacagcagtt gacccgtctt cctctgtacc
     7921 gccaggattg tacattactc agtcaagtcg atcttcattc tttatctaac caaatgggcg
     7981 tgatttttcc gattattttg tataatatcg tgacacttgc ttttgctggc tcattggtat
     8041 ttatgtattt ggatcgacac acaaaatcga tctaccgctt gattgattcg ctaaaacggc
     8101 tagaggaaga tggcttaaaa gctactgttc ctgtacaagg aagccatgaa gtccaattgc
     8161 tagggaatac gatcaatcaa ttgctaagta atgctcagca tcattatgaa cagacgatcc
     8221 aagacgaaaa gaagaaacgt tcgcttgaaa tccaaactat gcaagcacag atacagcccc
     8281 attttattta taatacgctg actagcttga aatttttgat ttggcagaaa aaaacagatg
     8341 aagcagttca aggaatcgac agtttcatta ctttactaag aagtacgatt gggaaaaaag
     8401 aagaagtgat tccagtcaag gaagaaatca aaagcgtaca aagttacatc gatcttttga
     8461 gtatgcgata tggcgaaaga atctcggcta aaataatgat tcctgatgaa ctgcagtcat
     8521 tatctatgcc gaatatgatc cttcaaccaa ttatcgaaaa cgcctatctt catgcttttc
     8581 acaataaaac gcaaggattc atcacggtgt acggcaaaac aaaaaatcag cttcttttat
     8641 tcgaagtgat tgataacggc gatggcttcg atactgaaaa taccaaacaa aaaaatgatc
     8701 atttttcagg aatcggcatc agccatgtag atgagcggat acgcctaatg tatggcgatg
     8761 catatggctt aacgatccaa tcagtgattg ggacaggaac gactgtaacg atttgtctac
     8821 cgcttcttcc taaaaatcaa tcagaatatc aaaaataaag aatagccaag cgctgatttt
     8881 tttattttaa gttaaataaa tcaatcagta ttcttgactt tcatctacgc gtctcttcta
     8941 ctgtcagcac tggataacag cccaatattt gactttctcc tccgagcatc ttgatttctt
     9001 ggagcgcatt ctctactaat gtcattggac ggtccaacaa caaatcgatc acgaaaaaat
     9061 attctcccaa gctagttttc aatggtcggg attcaatctt acttaagttg atttcccgcc
     9121 agccaaaagc tgccagcatt ttatgaagca tccctggccg attagcaggt agtgtcaaga
     9181 tgacactcat ttttactgga gctgactgct ggctggtcat ctttcggtca ccgacgatcc
     9241 aaaaacgtgt ctggttcaac tcattatctt ggatattctc agctaagatc tccaatccaa
     9301 catgctgtgc tgtttctaat gaagcaatcg ctgctgcgtc ttctttcgga tgttctgcta
     9361 catacattgc tgcagcagtt gttgattctg ttgctactaa aggaacatta ggataatgcg
     9421 tttctaaaaa ctgttgtgat tgtgctagtg cttgtggatg tgaaaggatt tttgtaatct
     9481 ttgttgctgg attgcctaaa agctgctgct tgataggaag aacgatttca cttttaactt
     9541 cgacttctgg atgtttggaa agaagatcga ttgtgtgatg aacagctcct tccaatgaat
     9601 tctctacagg aacgacagcc agatcgactt gtttgcgaaa caaagcgcgc aaacaaacag
     9661 ggatggaagc atacgcagtc aattgttctg taggaaatag ctgacaagcc gcctgataag
     9721 tgaatgaact ttctggccct agatagctga cgttcatatg cttttgatcc tttctataat
     9781 ggtatcgatg atcttttctg gtgtttgatt ggtcgtgttg attcgatgtg tcgcactttc
     9841 ttcataccaa gaaagtcgtt gtgccaataa tgcttttatt tcttctcttg tttttccaag
     9901 agctaaaggt cgaatagacg tttcatcttg ttggatacga tcaactagac agtccgcttc
     9961 tgcctctaag taaagaacaa tcggctgtgt ttttaaaaaa cgccgatttt ctgcactttg
    10021 gacaatccct ccaccagtcg cgataatttt gtcttcattt gacagctttc ttaaaatatc
    10081 tgactccact tttcgaaagc ttttttcacc gtaatagtga aaatattcgg caatcgacaa
    10141 ttggatatgt tcctcaatct ttgtatctaa atctacatac tctttttgta aacattgagc
    10201 taacccttta ccgattgttg tttttccagc gcccatgaaa ccaatcagca aaatgctagc
    10261 catgagcttc tcctcctgac acaagttctg ctaaatcgtc aaaaaatgcc ggataagaga
    10321 ttgaaacagc ttcagctcct tctagttcgc aaggtccatc agctatcaaa gcagctactt
    10381 gcagcatcat accgattcga tgatcgcctc tgctggacac ttttgcgcca tgcagaggtg
    10441 tcggcccatg gatgatcaga ccgtctgctg tcgcttttat ctttgctccc attttctgta
    10501 attcttctgc caccgcatca attctgttgg tttcttttac ttttaattct tctgcgtctt
    10561 tgatgatcgt gattccttct gcttgagtag ccactaaagc tagaatgggc aattcatcaa
    10621 tcagacgcgg aatgatttct ccaccaatcg ttgctttctt caaatgactc gtcttgacac
    10681 ttaaatcagc cgattggttc tgttcattgt gattcgtctg ggtaatcgct gcgcccatct
    10741 cctccaacac atctaagata ccagttcttg ttggattgac cccgacattt tttaacaaca
    10801 gttggctttc cggaacaaga agtcctgctg ctagaaagaa agcagctgat gaaatatcac
    10861 ctggaacagt tatctgttga ccagttaatt tttgcggtcc ggtcactatg atcgttttat
    10921 cttccactgt gatccttccg ccaaattgac gaatcatttc ttccgtatgg ttacgggaac
    10981 gttctttttc aacgattacg gatgtgcctt ctgcttgtaa tgccgcaaac agcaaagcag
    11041 atttgacttg cgcactggct acaggcatat tgtatcggat aggtgataaa gagcgggttc
    11101 ctgtcacagt gattggtaga tactggtcat tttcttctcc ttgcaagtca gcgcccatct
    11161 cgcgtaaagg ttccatcact cgccccattg gacgtttaga caacgaagca tccccgaaca
    11221 gagtagtcgt aaaaggctgt ccagccaaaa tacccatcaa taaacgtatg gtcgtaccgg
    11281 agtttcccat gtcaattgat tcggttggtt ggacaaatga gtggctaccg cgaccatgaa
    11341 tcttgatcac ctcttcctct tcttcgatct cgacacctaa ttgttggaag gcatgtaaag
    11401 tactcagaca atcttctgcg cgcaaaaaat tttgtatagt cgtcgttcct tcagcaattg
    11461 ctccaaacat gatgctgcga tgggaaattg atttatctgc tggtatccta acagtccctc
    11521 ttaatccatg tatttgttgt aataattgca tttatccagc ctcctatttg atccagcaat
    11581 gataactggt attagacata atgattcttt ttgcttgtaa caaatcctgt tggtttttaa
    11641 aactgatttg cagtattcct aaaatatctt ccctggtctc ttggatcttc aaattaatca
    11701 aagaaactcc cgcttttccg atcagtccag ttacttctgc aataacgcca ggttcatctg
    11761 gcacatcgat aaataaatca aaaaaggcag gaatcgcgcc ttgcttatgg acaggcaact
    11821 ggtctcgact ttcttttgcc cgattgaaaa aagaaaagat ttccgattgg ttctcagtca
    11881 tgatccattc tgctatttgt gtcatttctt gttgccaatc tgccaatcgt tccaataata
    11941 tctgcttatt gctcaacaaa atatctgtcc acatgacagg atctgaggaa gcgatcctag
    12001 tgatatctcg aaagcctcct gctgccagtt gtttggctcg cggatactct tgattgaatt
    12061 gttctgcctg attgactaag ccagctgcca gaatatgtgg aaaatgactt agcattcctg
    12121 ttatacggtc atgttctctt gctgtaagag ttacatattt tgctttggtt ccgcaaaata
    12181 gttcttgcag tttttcaaca tcttttttcg tcttttccaa aggtgtaaag atgtaatagg
    12241 cattttcaaa tagtttagca tctgctgcct gtatacctga tttatgagaa ccagccattg
    12301 gatgtccacc gacaaaccga agatttttct gctcagcgga tgtcacgact tgctgcttcg
    12361 tactgctgac atccgtaatg atgacgtgat cagacaagtt gattctttca aggacatcaa
    12421 gataagctaa ggttgtacgg acgggaacgg ctaataagat aacgtctgct tgttctgcac
    12481 ctgtttcaaa atcacttgca acgtaatcaa cgatctgctg ttctactgct tgctcacggg
    12541 tttttgactg actatcccaa ccaactacta tcagttctgg attggtctga cgaatacaaa
    12601 gtgccataga agcaccaatc agtccaagcc ccacgataaa aacatgttgt gccatcttat
    12661 ttgccccctt tttagtaatg tttcaagtag tcacggtact ccttcactgc agctttcaac
    12721 tcaacaagcg aatcacttgg gaattttgac aatagctctg tcgctaccac agtggctaca
    12781 acattttccg caacgacgct agctgcagga actgctgtac tatccgaacg ttctacactt
    12841 gctttgtaag gttcttttgt atcgatatca acactttgaa gtggcttgta caatgtagga
    12901 atgggtttca tgaccccgcg aatgacaagt ggttcgccat tggtcattcc gccttcgaag
    12961 ccgcctagat tattggtacg tcggaaatag cctctttctt cttcccatag gatctcatcc
    13021 atcacttctg agcctggtaa tgaaccagaa gtgaatccga ccccaaattc tacgccttta
    13081 aaggcattga tactaaccac agcttgtgcg attttggcat ctagcttcgt gtcccattgt
    13141 acgtagcttc ctaatccagc tggaacgcct ccaacgagca cttctacaat tcctccgatc
    13201 gtatctccat ttttctttgt ctgatcgatc aaatctttta ttctttgctc aactgctgga
    13261 tcaacaactc gtacttctga cgcttcagaa atttctttga tttcttgaat cgagatattt
    13321 ccaggtattt ctgcttggat tccaccaaga caaatcacgt gtcctgctac gatgatgtca
    13381 agttcttcca gaattttctt ggcaattgcg ccgattgcta cacgcatggt cgtctctctt
    13441 gcagaggacc gttcaagcac attccgaaga tctcgatgat ggtatttcat accgcctacc
    13501 aaatccgcgt gtcctggacg cggacgtgca actcgccgca gtgattggtc tttttcaggc
    13561 acttctgcta cagacatgac agttgtccaa tttttccaat ctttattttc taccactaaa
    13621 gtgacaggtg aaccaattgt tttgccgtga cggatgccag acgtgatccg aacacgatct
    13681 ttttcgatct tcatccttcc tcctctgccg taaccagctt gtctgcgagc gagttcgtga
    13741 ttgatttctt cttctgacag aggtaatcct gctggtagtc cttcaataat ggctgttaat
    13801 tctggtccat gggattctcc cgcagtcata taacgcatct tacagtcctc ctaagtaatc
    13861 tttgaatgtc tctgtgggaa caggaacgat ttttgcttgc ccaatctttt ctagcaagat
    13921 gatattcaat tggcttcctc ttgctttctt atcatgggtt atcgcgtgga ataattcatc
    13981 tggatgactt agatcgtacg taatcggcag atggaatttt tgaagtatcc tttctaaagc
    14041 agctgtcgat ccagttggag acaagccgat tttttctgca tgagaagtga tggctaccat
    14101 tccaatagct actccttctc catgactgac taccccatat cctgctgtat tttcaattgc
    14161 atggccaatt gtatgaccaa aatttaaaat aagtcgattt ccttggtcaa agacatcttc
    14221 ttccactact tttcgtttga tttccaacgt agcagtaatg attggaacag catgttccag
    14281 aagatctttt tcgtctttca gctcgttcag ctgtttccaa agcgttgcat ctgctatagc
    14341 tgctgatttg atgatttcag cgattccttc tcgtaaacga cgtaccggca aagtttttag
    14401 aacctcagga tcaatcaata caccagctgg ctggctaaat gttccgacaa ggtttttcgc
    14461 tttgtttgta ttgactgctg tcttcccgcc aatactagaa tctacttgtg caagtagcgt
    14521 agtcggaatt tgaacgaaat ggatcccacg catgaacgtc gaggccacga atgctgctag
    14581 atcacccacc attcctccac caagtgcaag tatcccatcg ctgcgggtaa aatcattatc
    14641 tgctaaaaaa tcgtataact cagctgcttg ttctaaagat ttactggtct cacccgccgg
    14701 tacgattgaa aggactggtt caaaattagc ttgtctcaat tggaacatga ttttttctgc
    14761 atatagtgga gcaacgtttg tatcagtgat cacagctact ttttgaggtc tccaaatcgt
    14821 ttgaagccat gatccagcct gttctagtgc gcctttttca atatgaatat cgtatttttg
    14881 gttatcaaca tgtacagtaa gcattcgttt cacttctttc ttttaaaaag ctggaattaa
    14941 tcgagtgtct gaatcttttc cattgcttct ttcatgatcg caacttcttt catcatgcgt
    15001 gagtaagtct tttcattcaa cgattgggga ccgtctgacc aagcatttgc aggatcagga
    15061 tggatctcta cgatcatgcc atctgctccg cttgccacac ccgcacgagc cattggtggt
    15121 acaagttccc aaattcctgt accatgactt ggatcaacaa tcacagggaa atgagttaat
    15181 tttttcatca atgggacagc acttaaatca aacgtattac gtgtcgctgt ttcgtaagtc
    15241 cgaatcccac gttcgataaa aatgacagga gatttgtctt cgactgcaat gtattctgca
    15301 gcattaagcc attcattgat cgtaccggaa attccacgtt tcaatccaac aggtttacct
    15361 gttttaccga cagcagatag cagtttaaaa ttttgcatat tacgtgcgcc aatctgtagg
    15421 atatcgctgt actcagccac catatcgata tgcccttcat ccatcacttc agtaatcact
    15481 ttcattccaa attcatcagc cgcttgacgg atgtatttca atccttcttc ttctaaacct
    15541 tggaaagcat atggagaagt acgtggtttg aaggcaccac ctcgcagaat ttttgcgccc
    15601 ccggctttgg ccattcgcgc acattctctg atttggtcta atccttcgat agaacaagga
    15661 ccagccatcg tcacgaaact gccgtcacca atttttactc cgtctacatc cacgactgta
    15721 ttgccaggat ggaattctcg agaagtcaat ttgtatgtgt ttaaaatacg cacagcattt
    15781 tctactcctt catagcgtgt aaatgctcct tcttgtaaac ttcgcgtatc accttttagt
    15841 ccaatcacta ctcgttctgt tccttgatcg attgagactt tgaatccttc tttttcgata
    15901 tgatcgacga ctttttttat ttgttcttcc gttgcttctt ttttcataat tactatcatt
    15961 gtttttcccg ctttcttttg tttacttatc tatcttggct gattttctct actttttcaa
    16021 ggtggtttta cattaattcc tactaaatga ccgtgtcctg ttgttctcat tttcgctcct
    16081 tctttcctac ttatgaccca gataatcaac aaaaaaagat gtccctgtag gaaattgcct
    16141 ctacagtgaa catcttcaac gagccttaaa aagcgctgca aaaatataaa atagccacta
    16201 tagatgaatt tctctacgtg gctgtaagtc gtgtttaaaa atctttagcc atcatagata
    16261 caaacctggt ttgtttgtac ccacgaagtc atgaatacaa acctatctaa aatagctaaa
    16321 gtaatacgta ttcagttttg taagttttct agatttcata aaaaatcgct ccaaactgat
    16381 gtaagaattg tacctagtat agacataaaa aaatcgactg tcaatcattt ttatgaaact
    16441 ttttttaatt ttctgatagt taactttgtt tttcagaaaa ataacaattg gttttattga
    16501 agaaaatagt catgaacaaa agtatctgag aactttttta ctctcttgtt catgacatat
    16561 agaataaagc agctttgtct tgtaaactcc gtttttcaaa taataatcta aagattgcta
    16621 ctgaataata aagccgattt catggtcctc ttcgctgctg attcggtaag gtgactcaac
    16681 atccgctccc cagctattga tacccccgac tcctcttact tttgcaaaaa tcgtaagaac
    16741 cgttcgtctt tctggcggta gttcttctat atgtgttgcc tgttccagtt cttctgcttt
    16801 gaatggtaaa gcggaaaaag caaatggcgt atcctgctgt tcaaatcgca acgaaaaatc
    16861 ttcttgatct ttatccgtat tatccaacgt ttgatttctt gttagttcta cccatttcgt
    16921 ttccatatgc atgccgcatt cttggggaac aaggtatggg gaaacgggga gtcctgccac
    16981 tttataaata ccttcgacac caccagccat ccgatccgga tatgtttccc ctgataggcc
    17041 ttcatactca aaaaattttg ctttcgttgg cataatgaac cttaaaccca atgcgggtaa
    17101 ttccggtaat cctttttgtc cataataatg catattgaca agcattttac cctctccagc
    17161 gatttggtag gaaattgtca cttgggtttt tggtactgtg ttgatttcca gtgtaaaaat
    17221 gatttcaacc gtttcagcgt attcattact tgaatattga ttatttactg gagcaatcgg
    17281 caaatcgatc tttttattgt ccaccagtac ttctatttct ttgacttttg gatacaatcc
    17341 gggtcctaac cacataccgg atttaaatcc aaatccgcta cctctgtcat tatcggtcgt
    17401 tgctcgccaa aaaactggaa gtgtttcccg gtatagccat tcttttccgt ttttattgag
    17461 cgattccagt ccgcctcttg tataagaaaa aatataatgc gtattccccg atttgacacc
    17521 taatgtacag tcgccgaaga cgattgcaac tttttccgta tccatatgac ttttatttac
    17581 tgtatttttc ataataatat cttacctcct gcatggcggg tttttcttgt ccatctgcaa
    17641 aaacgatccc attccctgag aattcatagt cacttggttt atcgtcaaag tcaccgccat
    17701 agcgcatcac ttctctgcct gtgattggat cagtcacttg gatcgcctga tctttaaaat
    17761 cccatatata gcctccttgg tacatttcat aacgatcaag caacgtcatg taagaatcca
    17821 ttccacctaa cgaattcccc atgtcatgca tatattcaca taagatgaac ggtttttccg
    17881 ctttattttc aaggtagtca atgatctctt ctggtggcgc atacatgcag ctttttacat
    17941 ctgaaatcga atcttcgaat gctggatttg gaaaattccc ttcataatgg acaagtcgat
    18001 cggggtctac ttttttgaag taatcgttca tttgtcgaat gttttcttcc gcataagatt
    18061 catttccaag agaccaaaaa agaatacttg gatggttttt taatgtttcg aaattcgttt
    18121 ttgctcgatc aagaacagcc gcctgccact gaggtactgc acctggtacg ttgtaagaag
    18181 gctcaaatgc gcccattttt tgccatgagc catgggattc gaggttcgtt tccgccatca
    18241 catggattcc tgcttggtcg cataaataat accaagtgat ctggttgggg taatgagatg
    18301 tccgtacgcc gttgatcttt gcatttttta tgatagccaa atctttttcc atatcttcta
    18361 tagtgatggc gcgtcctttt ttcggatgcc actcatgtcg atttaccccg ttgataatca
    18421 tccgttttcc attgaagtag atgattttat ctttgatttc gattttacga aaaccgaagg
    18481 aatagtcaat atactccgtt actctttttt cttcgtctat cgtctccaat tcaagcttat
    18541 ataaattggg ctgaaaatgc ccccacagtg ccacattttc aaatccatcg atgcgaatcg
    18601 tcatttgctc ttttaacgga tattgctgtt gatagatttc cttcccatca ggatctagta
    18661 tcgctacatt cagctgactg tttttggcta cttgctcaaa agacaaatct acttcaagtt
    18721 tgccattttg acatttgtca tctaagcgag gtttgatcca aagatcttct aaatggtttt
    18781 gtcctcgagc aactaagaag acatctctga agatcccaaa aaatcggaaa aagtcctgat
    18841 cttccaagaa tgcagctgta cttcttttgt aaacctccac agctagaata ttttcttctt
    18901 ccaaataagg tgttaaatca aattcagacg gagtgaacga atcttcggca tacccgataa
    18961 aatgaccatt caaccaaata aacattgctt gttctacacc agcaaaatga atcgagattt
    19021 gttttccctt tagaccttct tctaatcgaa aagtcgtcaa atacgaacca accggattgt
    19081 ctttagcagc tgaaaagatc ccttccgcct ctttctcagg gcttgtcgtg aaagggggac
    19141 ggcgaaatac atgtccctcc cacggataca tcgtattgat gtaatggatc ttgtcataat
    19201 ttgctaattc aatatgacta ggaacagtga tttcatcaaa agaacaggca tcaaaatctt
    19261 ttttataaaa tgtttttggt cgtaccgaag gatttggcga ataacaaaaa cgccatgatc
    19321 catttaaaga ctgtttaagg gaggatgtgg tttctacttc ttcccttgtt gcataacaaa
    19381 catgatcgct atgtgcattt gattggttca ctctaaaaac ttgtggatca tctaaccatt
    19441 ttatatttgc tttcatactt ttttccttct ctctcataac tttttgaaat gaagcagttc
    19501 tgtttggaaa tcaccttgaa aatcggggaa gtaaagtcca actgtcatca attcggcccc
    19561 cgaaaatatc cgcttcgagg tctgttcctg ataaagtgcc tcatcatcta accccatcag
    19621 atatacttca tggaacgctg gttgagctga agctagtatc ctccctaaaa acacaatagc
    19681 ttcatcttgt ttaggagaca caaacatcca tgcggcaata ttttcttcaa aaggactcgc
    19741 caaacgataa taatggccat actggatcac cggacgaatc gtttgatagg atataatttg
    19801 gtcttttact tgcttttctt cttcaggaga cagctttgtc aaatccagtt cataaccaaa
    19861 aacagcgctc atagctgttt ctcctcttgt tttcaacgaa gtagatctgt cggtctgatg
    19921 atttggtaca gctgatacat gagctgtgat ggacgaaatc ggataagcaa ggcttgttcc
    19981 atattggatt ttcatacgct ccactgcatc cgtgttatca cttgtccatg actgcggcat
    20041 ataataaata aacccggcat caaaccgtcc acctccacca gaacagcctt cccataagat
    20101 ttccggataa tcagttgtca aatgttcaag tagttcataa agcccgagca tataacgatg
    20161 cgctacttct ccttgtctct gtgggtcaag ggtaatcgaa tagacatctg aaagcgaacg
    20221 gttcatatcc catttgatgt agtcaagagg aatcgtatct aaaatctttc tcatttgttt
    20281 ctcaatcgtc tgccgaacat ctaaccttgt aaaatccagt acatgttgtg atctggaagc
    20341 agaaggcatt ctacctggct cctgcataag aaaatcagga tgtgttcgat acagttcaga
    20401 atcaaccgag atcatttcag gttcaaacca taaaccaaat ttcaatcctt tgctatgagc
    20461 ataatctgct atttcacgta atccatttgt caacttgcct tcatactcga accagtctcc
    20521 taatgaagaa ttgtccgcat ctctttttcc aaaccagcca tcatctaata cgaacatttc
    20581 gatgcctagc tcctttgctt catcaatgat cgccttgatt ttttcactat tgaagtcaaa
    20641 gtaagtggct tcccagttat tgaccagaat cggccgttca gcaaattgat gacgacctct
    20701 tgcgattctt tcacgtagca actcatggtg gatctgactc attttgttca atccctgact
    20761 tgagtaagaa aggatcattt caggtgtttg aaaagactgg cctgcattca attcccacga
    20821 aaaacgatgg ctgttgatcc caccgacgac tctcaattga ttgatctgat ctttttcaag
    20881 ttcgaaagaa tggttgccgg aataaacaaa ttgaagtccg atcgcttctc cagtattttc
    20941 tgtcgtatga tgatgaacta aagcaataaa gttattcata tggtgactgc tcgttcctcg
    21001 acggctttca aatacttttg ttccagataa aacagattgc cgtgaaatct gtctttcatt
    21061 gacatgtgct cctggcaaag caatgacttc atcaaatcgc ctagtatggg caaagtctag
    21121 ctggaaacta gccgcttttt ctagtttgat tgtttctccg gtttcatttc ttatctttac
    21181 agatcgtgtg atcactgctc tatcagtaaa tatggtatag aacaaatcga aataaatcgc
    21241 tgtttctcta tccttaagtg aaatcaccaa cgtttgtgcc tctgacttgt ccatcacata
    21301 agtttgaggc aagccttcga tctctggctt cccatctgta atataatggc tgtcatatct
    21361 cagatccagt aagtctgatc cattcttgcg ctgaatgatc gatgcgggcg ttcgaaagtc
    21421 catgctgcca tgagaagagt attcttgcgg tagtgtatct ttcgacagag accggtcagg
    21481 atttaaggga acatttcctg aaaatccacg atctcttcgg ggataatttt tattgtcttt
    21541 gtactgcttg actggtttgc caaaataaat atgcgccaat acattgcttt cttctatctt
    21601 tatcagataa gaaagaaatg tatttcgtaa atggaagact cggctctttt catcaaataa
    21661 gatgaaattt ttatctgttt ttctatgcgt aatatccatc atttcactcc caacaaaacg
    21721 gaaaccgttt actttttttt ataattcctg tatactgttt actaaacaat tttgtcaata
    21781 aaaatttttt atttttttga ggagtgtgta aaaatggttg gaattagaga tgtcgcacga
    21841 aaggcaaatg tctctcctgc tacagtttcc cgtgtgttga ataacgatca gacgatccat
    21901 gtggctcaga ctactcggca acggatagta gaggcagctg ctgcgttgga ttatgacatc
    21961 agtactcgta aatatatcaa aaagagaatg ccttcgattg gtgtgatctc tacgatcggc
    22021 aaagcgagcg aagaacagga tatctattac aaacatttgc gagtagggat ggaagaagaa
    22081 gctcgacgtc tgcatttagg gatgaatcga acctataatc tatctgataa tcctaaacaa
    22141 tgggaagatc tagatcagtt aggtgcggtg atcgttgttg ggactgttac gaaagaatcg
    22201 attaccgcct tgttaaaaca aaacatgcaa atagtcgtcg tggataaccc tgacatccag
    22261 caagaggtcg atatggtgta tgcagatctt gaacggatga ctaaaaccgt actggaattg
    22321 ttcttagaaa atggtcataa aagaatagcc tatatcgggg gatataaagt cgatatggat
    22381 gaattcggtg ggaagcagat ttctataaat gaaaagagga tgcgtgccta tcagcatttt
    22441 atgatcgatc atcacttaga aaaatatatg tcttatcatc taggtggctg gacagaaaaa
    22501 caaggtgagg tacttgcgac taatctatta gaaaatgaat cccctcttcc tactgccatt
    22561 cttgtcggga gcgatccaat ggcaattggt gtctatcggg cattgaaaca aaaaaatatc
    22621 caaattggag aagagattgt tgtagcaagc tttgataata tcgagacaat cgcaaacctc
    22681 tcccctggct tgaccagtgt tcaaatcaat gcgaaatcaa ttgggcaagc agctgttcga
    22741 atggcattgg agcgaattga cggtatacgg gacgaaccaa tcattttgac ttatcctgca
    22801 cgattgattg ttagggaaag ttttatccca agaacaaaaa ggtattgaaa gagaggttgt
    22861 gaaaagccgc ttcgtttctt catactcaaa acgaaacggc tttttcacaa cctcttactt
    22921 atacttattc acaaaatcca atgtctgctg gtaacaattt tgcgattctt ttagcgtgta
    22981 atcaaattgg tattcatggg taatctcttt ttttgtatct gtataaaaca aggattgcac
    23041 gggtaccttt aattctgtca accgattttc taatgcttga ccttgctcct ggaatgagta
    23101 tgcatttccg tcagttatat acgttggcgg aaattcctta gaaacatggt cagccacact
    23161 agcttctttc aattgctcac tcgttttcca atcttttgtt ccaatcaacg accacgcaac
    23221 ggttttgacg aaaaacttca taaatcgatc atccgaatgc tgctgtgctg ttagcttcaa
    23281 atcaacaggt ccgcaataag aaatggttcc tttcagacta tcttttggaa gtagatgttt
    23341 gattttcatt tgtttagcat acgcatcatt cgtttgtata gctgcatatt gcaatgcgat
    23401 ttgtgaacct gcactatctc caccaaaaaa taattgttcc atgtctagca atttgtcgtc
    23461 tttttcttca gctaataaat ctttgatcag ttcattcact tgaagaactt gattagggta
    23521 ttctgaatca ggcgctaatt cataattcat cgcaaccact gcgatgttgg cgtctgaaac
    23581 taattttgtc gcaaattctt tgacacccga tttatctccg ccgacgaatc ctccaccatg
    23641 tacccaaaat agaacaggaa caggcccctt gctgtttttt ggatagtaga tgtcataagt
    23701 attctcttta tacttggact catacgtcaa atctgttttt ttcgccacgt ttttttctgc
    23761 ttgatcgaac gtttttttat cagtgatttg aacagtattt gaaaacatat ggttgatcaa
    23821 gaatgctcca ggtctggggg aaagctggaa ggcaagaaag attgcaaacg cgatcccaag
    23881 aatgattccg aagaaccatc cgataaattt aagaattttc tttttcataa ggctccctca
    23941 gtagttagta ttaagcattc tgattatacc atagtgttat ttcctatcaa gctctcctag
    24001 atgatttaat gattttcccc aaacttcttc tgcaatgctt ttgacaaaag aaattttttc
    24061 cattgttctt caaacaaaac attttcttct tctgttgaag caaatccaca ttgaggactt
    24121 aagcacaaac gatccaaagg cagatatcgt gtcgcttcgt agatccggtc aatcacttgc
    24181 tggtgctctt ccatttcgcc atttttcgtt acagtcaaaa tctaagacaa ctaatttatc
    24241 ttcagagact ttctctaaaa gaacaaaact cccagcacgt tcactatcgt attctaaata
    24301 ataggcacgg acattttctt gagtaaaata atgggtctgc tacgtcgtca tcccccctga
    24361 tgccgccaaa atcgaataat aatttccgcg tcaaacatgt atatttagtg ttgaatcatt
    24421 tgagaatatg aaaataaaaa tctttttgat caatcagtta atcaaattga aataacgaaa
    24481 aatctgagac agaatgacgt tcattctgac ccagatttga ttttgcacaa ctcaacagtc
    24541 cttattggca aatcgccaaa ataatgtaga ctaactaaaa agactaagaa acaataacaa
    24601 aagttaacac ttcttagtct ttctttagca cgtctattta ttaaagattt tagggccttt
    24661 tctcatgttt ttacgatcat tcttaggtgt ttccatttgt ttggatcggc ctccaccttg
    24721 tttctttttg atcaattctt tcattttttc tttttggtcc atttttattc tcctttaacg
    24781 aatgaagcga ctaattcgat atgatgggtt tgcgggaaaa gatctacagg ctgaactttc
    24841 ttcacggcat aacctgcttc tgcaaataac ttagcatctc ttgcaaaagt tgccggatta
    24901 catgagatat agacgattcg ttctggtgac atttcgattg ctgattcaat aaaatgatga
    24961 tctaatcctt ttcgtggagg gtcaactacg atcacagatg gtttgatccc tttttcttgc
    25021 cattttttca taactttttc tgcttttccg acttcataat gaacattttc gattttatta
    25081 tttaaagcat tgaactgtgc atcttcaatt gcttctggaa taacttccat cccatacacc
    25141 ttatccactc tgtctgctaa agacaagcca atcgtaccta ttcctgaata agcgtcgatc
    25201 acaatgtctt ttttatctaa ctcggcaaac tcgatggctg tttggtacag atactctgct
    25261 tgttgagtat ttacttgata aaatgacttt gctgagattc gataggtttt tcctaatagc
    25321 tgatcttcga tatagtctct gccatataaa actttttctt tgtctcctaa aatcacattt
    25381 gttttttctt cattgatatt ttggatgacc gaaaccactt ctggcaattc ttcatgaata
    25441 gcttcagcaa tttccttgga tttaaagaat ttttctttac gagtaactaa aacgaccatc
    25501 atttcatggg aataatgacc acgacggata atgatgtgac gaagctgtcc ttcatttttt
    25561 tcttcattat atcctcgtac ttggaaccgt tgcaagatat ctctgactac tttgattgct
    25621 tgatcgatga caggatcttg gatataaaaa tcttcgatag ggactagatc atggctattc
    25681 tttttgtaaa aaccggtggc taactgtcca tcgatacggc gaacagggat ctgtgcttta
    25741 ttacggtagc cgaatggttc tgccattccg accgttggaa gtacaggaac ttctggcatc
    25801 ttagcaatct tcgccattac atgctctact tgtttttgtt tgaagacaag ctgttgttca
    25861 tacttcaaat gggctaaagg tgcgatacct atgcgaagta atgcattgtt ttctacaggc
    25921 tgacgatcag gtgattgtgt aataaatgtt tcgacttttc catatccaaa cttattgcct
    25981 gcttttacta ctagtatttc tacttcttct ccagggagtg cattttcgat aaataatgga
    26041 taaccatcga tttttgctac ccccattcct tcataactca agtcgacgat ttcgactgta
    26101 tatcgttcgt ttttctttac tatttgctgt tctgtcatat tttgctcctt taagcctatt
    26161 tttcaatcct actgaactct actataaatc agctaattac acaactaaaa ccggcactga
    26221 ccttactacg ttcagcaccg gcttttgatt aattcttttg attaggtgtt tcgtctgcta
    26281 tttttccgtc accgtcgata tcttcatccg ttaaacgttc gacttctttt acgaattctt
    26341 tgctgacaac ctctaattct tcttcatctg tacctgtgat tgcttcatca ggaatctgat
    26401 cggtattggc aaagaactca atgtgttgat gaagattttg gaaatgcatc ggtgcttccc
    26461 cgccgtattc accatctaag ttgatcatta ctggtcgatt gatatcatct tctgtttcaa
    26521 cgaacaaact gcttgttttg gtatagatta ttcttggatc atcgacatgt cgaccgccat
    26581 tcagcatgag tgctgcaata tgcaagatct caaagatatt tgctgtcttc acaatgatta
    26641 aagaaaactt cccatcatcc aatttggcat ctggcgcgat ctgttcgaat cctccaacag
    26701 agttcgtcag ccctaggaaa aacatcgaag catttccgat atattcccct tcatcatact
    26761 tcaagcgcat tttgattggc tttactcgag ggagcatctc tgctcctttc gcaagataag
    26821 caagataacc aaaaatactc tttagttctg atggaacttc ataagtcaat tctgtcaaat
    26881 agccacctgc agcgatattg atgaagtaac tgttatcaga aaggccgatg tccattttta
    26941 cggtttggtg tttttttatc acttctgctg ctgctttaat attatctcta ggaattttta
    27001 atgctcgagc atagtcgttc gttgtaccag ctgggatgat ggccatttta ggacggtgtt
    27061 caagcggagc gattccatta accacttcgt tgattgtgcc atcgccacca gctgccacta
    27121 ctagatcaaa acctagttct gcgactctac atgcttcatt cttggctgaa tctggttctg
    27181 gtgtagttgc ataggcgctg gcttcaaatc ctgcttcttc taatgcctgt aggatatccg
    27241 ccaaattttt tttcaataat tcttttcccg aggtggggtt ataaattacg cgtgcttttt
    27301 tcattttcag cctctatcgt ttcgctaatt catctttaag tagcttattc acgacaccag
    27361 gatttgcttg tcctttagtt gctttcatga tttgaccgac taggaagcca accgcacgat
    27421 cttttccatt tttgaaatca tcgacagatt gttggttgtt atccaaaatt tcattaatga
    27481 ttggcagtag tttgttcgga tcagataatt gcacccaacc atttttttca actacttctt
    27541 gggcttctcc gccatttgtg atcaattcac ggaagacttt tttggcgatt tttgagctga
    27601 tcgttccatc ggcgatcaaa cgaatcattc ctgctaagtt ctcaggtgtc aattttgtct
    27661 cagctaattc cagtttctca ctgttcaagt aagcagaaac ttcacccatc aaccagttag
    27721 atgcttgctt ggcatctgca ccattttcca aaacagcttc aaaaaaatca gacatttctt
    27781 ttgtaagtgt caataccatt gcgtcatact ctggaagtcc aaactcacgg atatagcgtt
    27841 cgcgacgaga agctggcatc tcaggcaaac tagcttttac tttttgaatc cattcgtcat
    27901 cgatcacgaa tttcgggata tcaggctctg ggaagtaacg ataatcacta gacccttctt
    27961 tgacacgcat caaaactgtt ttattcgttg tttcgtcaaa acggcgtgtt tcttgttgga
    28021 tttctcctcc tgaaagaaga acttttgctt gacgtttttc ttcaaaagct aaaccttttt
    28081 tcacgaaaga taaagagttc aagtttttca attctgcttt cgtaccgaac tcttcttgtc
    28141 cataagggcg aagagaaata ttggcatcac agcgcataga gccttcttcc attttcacat
    28201 cgctgacttc tgtaaattgg ataattgatc gcaatgcttc tagataagcg tatgcttctt
    28261 ctggtgaacg catatcagct tcagaaacga tttcgatcaa aggcgtgcct tgtcggttca
    28321 aatcaacgta agaataaccg ccaataccat gcatgttttt tccagcatct tcttctaaat
    28381 ggacacgttc gatccggatt tttttctttt taccttctac ttcgatttcg atccatccgt
    28441 catgaccgat tggttgatca aattgggaaa tttgatatgc ttttggatta tctgggtaaa
    28501 agtaattttt ccggtcaaaa tgagtatctt tagagatttc acagtttaat gccaaagctg
    28561 ctttcatccc gaattccagc gcagctttat tcattactgg cagcacgcct ggataacccc
    28621 agtcgatcac gtttgtattg ctgtttggtt ctgcaccaaa atgagctggc gctggagaga
    28681 aaatttttga gttggttttt aactcgacgt ggacttctag tccaatgact gtttcaaaat
    28741 tcattcgtct ttccctccta aaatagctgg ttgttgttta tggaattctg ttgcttgttc
    28801 aaacgcatat gccgcttgat acatcgtttt ttcatcaaaa taattaccga tgatctgcaa
    28861 tccaactggt aatccttctg ataaacctgc cggtacagac atacctggaa gtccagccaa
    28921 gttgacaggg atcgttaaga tgtcactcat gtacattgtg attggatcgt taatgttctc
    28981 accgattccg aatgcaactg ttggtgaaga tggcccgatg atcaagtcat aatctgcaaa
    29041 gactttttca aaatctcgtt tgatcaatgt acggacttgt cctgcttttt tgaagtaagc
    29101 gtcataataa cccgcgctta aggagaatgt acctaacatg atacggcgtt ttacttcttc
    29161 accgaatcct tctgaacgtg agttgacata tacatcatct aatgatttca cattttcaga
    29221 gcgataaccg tagcggatac catcgaatcg ttgcaaattg gaacttgctt ctgatgaggc
    29281 aatgatataa taaacagcca caccgtattt tgaatgaggc aagctgactt cttccacaac
    29341 agcacctaat gaacggaatg tttctgctgc tttcaataca gcttctttta cgccaggggc
    29401 cacaccttcg cctaagtatt ctttaggtaa agcaatcttc attcctttga tgtcgcctgt
    29461 cagtccctct gcaaaatcag gaactgaaac gcctgaagaa gtaccgtctt tttcgtcata
    29521 accgctgata gctgtcaatg ctaatgcatt atctttcacc gtacgggtca ttggtccgat
    29581 ttggtcaaga gatgaagcaa aggcaatcaa accaaaacgt gagacacggc cataagtagg
    29641 tttcatccct acgatgccgt taaaagctgc tggttggcgg atacttcccc cagtatcact
    29701 acctaaagaa actggaattt gtccagctgc aactgctgct gcagaaccac cagaagagcc
    29761 accaggtact tttgttgggt cccaagcatt ttttgttttc ttgaaataag aagtttctgt
    29821 acttccaccc atagcaaatt catccatgtt tagcttacca acagggatca tatctgcttg
    29881 atagatttta tccatgaccg ttgcatcata aattggttca aaattgtaaa ggatcttaga
    29941 tgcagcggtt gtcaagatat cttttgttac gatattatcc ttgataccaa ttggaatacc
    30001 agcaaggacg ttgctttcat caatcccttt ttcatctaaa gcttttgcct gttccaatgc
    30061 tttttcttcg ttcaatgtaa tgaatgcctc gacctcaggc tctgtttctt tgattcgttc
    30121 aaatgttgtt tttgtcaaat cttgtacagt gatttctttc gatacaagca tatgatgcag
    30181 ttcttctaga gaacgattgt gtaattctgt cattatgcgc cagcctcccc attatcgatg
    30241 attgcgggta ctttgataaa gccgtcttct gtttccggca catttttcaa taattcatcg
    30301 cggttccatc cagcttgcgc ccgatcttgt cgcatcacgt ttactgtttc aaccacattc
    30361 gacgtaaatg gaacaccttc cgtatctact tcttccagca tttcaaccat atcgatgatt
    30421 ttacctaatt ggtcggtaaa acttgcaagt tcttcatctg cgaacgcaag ttttgctaat
    30481 ttggcaacgt gtttcacttg ttcttcacta atcgccatac taccccttct ttctaaacga
    30541 gttaaaaact ttgctctcta ttttaacaca agagtgccgt gtctgaaatt gtttattgaa
    30601 atttgtctga aaaaactcat ttttcattct tctactgagc gacttttagc caatgcatct
    30661 gccatttgat cttctgtcca aacttcgatt cctaattctt gtgctttggt cagcttgctg
    30721 ccagcatctt ctcctgctac cacgatatcc gtttttttcg atacgctacc agtcactttc
    30781 ccgccaagat tttcgatcgt ctcttttgct tcttcacgag taaatcgtgt tagtttacca
    30841 gtaaggacta ctgttttttc tttaaaagga gactcgactt cttgcaattg ggcatttcga
    30901 agccctttgt attcgaaatt gacgcctgct tgttttaatt cattcatcaa ttcatggact
    30961 tcttcgttac tgaaataagt gactacactg tcggcaatga tatcaccgat cgtatcaagc
    31021 gcaataatcg actcatagtc acttttactc aatgtttcta gatcaccgaa atgttctgct
    31081 aaaatttttg ctgctttcgc tccgacatgt cggattccta acccgaaaat caaccgctcg
    31141 accgaattat ctttgctgtt gtcgattgct gtcaaaatat tatttgctga tttttctttt
    31201 attttgtcca acgtcaataa ctcttcttct gtcaatttgt aaagatcagc aacatctgct
    31261 actagttttt tatcatacat ctgttctagt acgcgcggcc ccaacccatc gatattcatc
    31321 gcatttcttg aaacaaagtg gttcaaacct tctttcattt gagcagggca tttaggattg
    31381 atgcaccgta aggctacttc ttcatccaaa tggacaagtt cacttccaca aactggacaa
    31441 tgtgtaggca attggtattc ttcgctgtct ttaggtcgtt tatctaacac gacttgcgaa
    31501 acttctggga tgatgtctcc cgctttataa atcagaactg tatctttcaa gcggatatcc
    31561 ttcaacttaa tataatcacc gttatgcagg ctggcacggc ttactgtcgt tcctgctact
    31621 cggactggtg tcatgatagc tgttggcgtt actacccctg ttcgaccgac tgtccattcg
    31681 atgttttcga ttagcgtttc aacttcttct ggcggaaact tgtaagctgc tgcccatctc
    31741 ggtgctttga tagtaaaacc aagttgatct tgtagcgaga attcattcac tttgatgacg
    31801 attccatcga tttcataggg caaatccaca cgtttgtcat gatattcttc aatgtatgac
    31861 cacacttcat caatcgaatg acatagacgt ttttcccgat tcgtgtggaa tccaattttt
    31921 tctagctctt ctagcgcatc atactgggtt tttgcttgca taggaccgaa atcagctact
    31981 gtgtataaaa aagtgtccag atttcttttc gctgtgattt tagaatctaa ctgacgcaaa
    32041 gaaccagctg cggcgttccg aggattcgca aagatatctt ttccctctgc ttctctgtct
    32101 tggttcaatt ggacaaatga ccgttttggc atgaaacatt cgcctcttac ttcgatgttc
    32161 attggttctt ttaatttgat tgggaccgac cggacagttt ttaggttttc agtgatgttt
    32221 tctcctacag ttccatcccc gcgagtcgca cctctcacga aatttccatc ttcataacgc
    32281 aaagaaacgg aaagtccatc tatttttagt tcacagcaat agtcaactac acgtcccact
    32341 gctttttgta cacgttgatc aaaagctatc agctcttctt tgctgaaaac gtcgttcaaa
    32401 ctgtaaagcg gaatatcatg agtgactttt tcaaaacctt ctaatacttt tccgcctact
    32461 cgttgagtgg gagaatcaga cgtgatcaaa tcaggatatt gttcctcgat tgctactaat
    32521 tctgcgtatt ctttatcata aacatagtct tccactgttg gtttatcttc tacgtagtat
    32581 tcgcgagacc attggttcag tcttgtacga agttcttctg ctcgtttagc agcttcgtca
    32641 atcgtcattt catgttccat catttaccct ccactactaa gctttttcaa ttggcgcaaa
    32701 ggcagccaaa agacgtttta ctccttgact tgggaaagcc acatctagtt ctagatcctt
    32761 cgacgtcccg cttactcgga cgactgttcc tagaccccat tttttgtgtt tgactttatc
    32821 tccaggtttc cagctgttat tttcccctcc agtagcactt ttagagggaa caaccgctgt
    32881 ctctggctgt ttatagctat atgctgtttt tttagcgaat acaggcgttt ttttaggcgt
    32941 aggacgcatg ccttcgattt ccagtaactc ctgatcgatt tcttctacaa aacgagaagg
    33001 tcggttatat tgcgtccgtc catataatgt tctggagaaa gcatttgtta aatacaacgc
    33061 ttcttctgct cgcgtgatcc ccacatatgc gagacggcgt tcttcctcca gttcgctttc
    33121 ttccatcaat gctcgagata aaggaaaaac cccctcttct agaccaatca ggaatacaac
    33181 tggaaattct aatcctttgg ctgcatgcaa agtcatcaat gtcacttggg aagcatcttc
    33241 ttctaaattg tcaatgtcgg aaacaagtgc cagatcattt aagaagaccg ttagtttttc
    33301 ttctggtgca tcagcgtctt cttcattttg ctgttcgaat tgtttgtcga attcttgtgt
    33361 gaccgttaaa aattcctcca agttttcgat gcgtgcttgt gcttctaatg tattttggat
    33421 ttttaagtct tctagatatc cgctacgatc caaaacctct ttcgtcaatt ctgtcactgt
    33481 caagtatggg atcatctgag tgacttcttg gatcatctct ccaaatgcac ctaattgctg
    33541 tcctgctttt ccaccgatat ttgccagatc cacattttgc gctgcttcaa gcagtggcca
    33601 ttcatgaaga gaagcaaaac tgcgtagttt ttcgatagat cctggtccga tgcctcgttt
    33661 gggcgaatta acgatacgct caaaactgat tgaatcttga ggatttacca gcacattcaa
    33721 atacgctaaa atatctttga tttctttacg atcgtagaac ttgtgccctc caaccatttt
    33781 ataaggaata tttgctttca acagagtttc ttccattaca cgagattgtg cgttcgtccg
    33841 gtacaagatg gcaaagtcgc cgtaattccg atgattgctt cgaatctctt cttgcatccg
    33901 gtctacgata aaacgggttt cgtctcgttc attatctgca cggtagtaag tgatcttgtt
    33961 tccttctttg ttttctgtcc acaagttttt tggttttcga ttgctattgt tttcaatgac
    34021 ttggtttgct gcgctaagga tgtttttcgt ggagcgatag ttttgttcta gcaaaattac
    34081 tgcagcatct ggataatctt tttcaaaatc caagatgttc tgcatatctg caccgcgcca
    34141 gccgtaaata ctttgatctg catctcccac gacgcacagg ttcctaaatc tacctgccaa
    34201 taaattgact agcgtatact gtgcgtgatt tgtatcttgg tactcatcta catgaatata
    34261 atggaatttg ttttgataat acgtgagtga atcaggatgt tcttcaaata atcgaatcgt
    34321 attcatgatc aggtcgtcaa agtccataca ttgattatat tgaagctctt tttggtaagc
    34381 agcataacat tttgccgcaa tctcttcgta ataactgcct tgcatttttg caaaatcctg
    34441 aggggtctgc aggctgtttt ttgcttgaga gattgttcct aatatacttc gaggatcata
    34501 ttttttcgga tcaatgttca gttcttttag tatccgtttc atcaaggtca gctgttcaga
    34561 agaatcgatg attgtaaagt tccggttata cccgataaaa tcaacatctc ttcttaaaat
    34621 acgcacacac atcgagtgaa aagtcgatac ccagacatct tcgcctccag atgcgagaat
    34681 cgcattgaca cgttctttca tttcttttgc agccttattg gtaaaagtaa tcgctaggat
    34741 gttccaagga ttgacttctt tttcctcaat caaataggca atgcggtgtg tcagcactct
    34801 cgtctttcca cttcctgcac ctgccattag cagtaatggt ccgtcagtgt gaagaacagc
    34861 ttctttctga cgggggttca tcccgtttaa taattcagct ttggatgaca ttaacaacat
    34921 cctttctttt atcaatcttg ttaattatag cattttcttg gctttcatta aactagctag
    34981 ttcaaaatca tttgtttctt tttatggatt tttccttgtc aattcgttga tttcgcgcta
    35041 aattaaaaga aagcgaggcg tttgaatgga aaaaaaagga aaaatcaaga aattagataa
    35101 gactcagata tattttattc taaaaggaat atttgttgga ctgctgacag gaatcgtcgt
    35161 tagcctgttt cgattatcta tcgaaaagct aagcgattta gttcgaagta tctatgaaat
    35221 gagtcgggaa cagccgattt acttaattgg cctagctatt tgctgcatcc ttgcagcatt
    35281 tttcgtgggt tatctggtca aaaatgaacc ggatatcaag ggaagcggga ttccacaagt
    35341 agtgggacag cttcgcgggg aattatcaat gaactggttc tctgttttat ggaaaaagtt
    35401 cattggcggt gtactttcag ttggtgcggg cctttttcta ggtagagaag gaccttctat
    35461 acagcttggc gcatccattg gacaaggagc tggacaattg tttcggagtc catcttcaga
    35521 agaaaagatt ttaatttcta gcggtgcaag tagcggactt gcagcagcat tcaatgctcc
    35581 tatcgcagga ttactctttg ttttagaaga aatccatcac tccttttctc cacttgtttg
    35641 gctgacatca tttgcttcag caatcactgc taacttcgtc tctctttact ttttcggctt
    35701 gagacctgtt ttatatctag gtgaccttcc ttctttgccg ttgaaatatt atggttcctt
    35761 agtgatctta ggagtacttc taggcatctt aggttttatt tatcagaatg tcttgctggc
    35821 tttgccaaaa ttttacaacc gattgccttt accagcttat tttcacgggt tcgttccttt
    35881 cttgctgatt ttgccaattg gttatttatg gcctagtact ctaggcggag gcaatcaaat
    35941 cgttttagct tttggacatg cttccttgcc aatcattatg attatcggtt tgtttgttct
    36001 tcgattcgtt ttttccatga tttcttacgg tgggggtctt ccagggggga ttttccttcc
    36061 gatattgaca ttgggggctt taatcggtag cttttatgga aacattatgg ttgatttcgg
    36121 gatggatcca atctacatca aggattttgt cgttattgcc atggctggtt atttcacagc
    36181 gattggaaaa gcaccgctga cagcaattat tttagtgaca gaaatggttg gctctcttaa
    36241 tcatctgatg cctttaggat tggctgctct tgttgcttat atcgtcaatg accttctcgg
    36301 cggtaatcca atttacgaat cattattgga acgtctactt ggaaatactc agaccgcctc
    36361 actaaaaggt gcaaggacaa cttttgattt tccagtaacg tcagagagtt ctttagacgg
    36421 attaatggta cgagacttca attggcctaa aaatatgctg ctaatctcta tacgcagagg
    36481 aaatcaggaa atcttgactc acggtgatac gatcatgaat gtaggagata ttttggttat
    36541 cttgactgat gaagcaaact tgccaagaat caagcaggaa atcgaggcta aatcttctat
    36601 cacaaatttg aagcaatcat tttaacgaac atggaaaaac tattttacaa caactattca
    36661 tctacactac ttgattctat atgattctat aaaaaaagaa gaggctggga aaacccagcc
    36721 tcttcttttt tatttctatg cttctgctac tgcaggagct tttctcaata aaccataaat
    36781 cacaccagat acgactgctc cgatagcaat aaacaccaaa tacatgaccg ggtgactcag
    36841 taagaataca acgaaaatcc cgccgtgagg agccagtaat ttgattccga aagctccgac
    36901 taatccgcca gttaaagcag aaccaataat aaaacttgga atggcacgaa tcgggtcagc
    36961 tgctgcaaat ggaatcgatc cttccgtgac aaacgatagt cccatcacga tgttcgtcaa
    37021 accagcatct tgatctgttt tgctaaattt attcttaaac aaacgtgtag caacgaaaat
    37081 tgccaatgga ggtaccattc ctccagccat aacagaagcc atcacgacac ttccgccagt
    37141 tgcaacagtc gtagctaaag ttgctgtacc aaacacataa gcggctttgt tgattggacc
    37201 gcctaaatct gccgccatca ttccagctaa taatgcacca agtaaagcag cattcgaacc
    37261 actcatactt gtcaagaaac cgttcaatgc atcattgatt gctttcatag ggacattgac
    37321 cagcaacatc aagaaaccag taatcagtaa gccaaacact ggataaaaga ggatcgtttt
    37381 aattccatcc aatgattttg gtaagttctt gaataattta cgtaaaaata cgatgacata
    37441 accagctaag aatccaccaa tcagtgcacc taagaatcct gcaccgcctg tattagctaa
    37501 tgcaccagca gcaaatccaa cgatcaaacc agggcggtca ccgatacttg aagcaataaa
    37561 tcctgctaat accggcagca tgaaaccaaa tgcagcttgt ccgatttggt tgaaccagct
    37621 agcagcttga ttgtaattcc ctagatttga taattgatca tgtggcacgc caataaattg
    37681 gtcgatcatg aaagaaagcg caatagcgat tcctccacca ataacgaaag gtaacatgtg
    37741 tgatacgccg ttcatcaaat ctttgtaaat tctttgtccg atagtaccat ccgcatttcc
    37801 ttcttctgaa ctgctttctt ttccatcacc atggaatact ggtgcagtac cactaagggc
    37861 tagattgatc aactcttctg gtttacggat accatcactg acaggacgat tgatcagttc
    37921 ttttccgtca aagcgattca tctctacttt tttgtctgct gcaacgatta caccttctgc
    37981 acgggcgatg tcttcagcag tcaatcggtt tttgattcct tctgaaccgt tcgtttcgac
    38041 tttgatctcc acacccattt ctttcgcttt tttcttcaaa gcatcttctg ccatgtaagt
    38101 gtgagcgatc cctgttggac aagctgtaac ggcaacaacg aattttcttt ctggatgagc
    38161 agaagcttct gcagcttgtg tttcttgttc tgctttttct tctgcttctt tttgctcttc
    38221 agctgtttgg aacaagtctt ggacttcttc tggtgtattt gcttctttta attttcctac
    38281 gaaatctgca tcaatcagca atcgagatag tgctgctaat gcttgcaaat gtgtgtcgtt
    38341 tgcgccttct ggagctgcga tcatgaaaaa cagataagtt ggctgtccgt ctaaggcttc
    38401 ataatctact cctttttggc tttttgcgaa taaaactgtt gcttctttaa ctgcactgtt
    38461 tttcgcgtga ggcatggcga ttccatcacc aagacctgta gaagtttgtg cttcgcgggc
    38521 tagaattcct tctttatatg tttcgatatc ggaaatccgg ccggcatcaa acattttttg
    38581 gaccatttca tcgattgcgc cttttttatc agtcgcttgc aaatccatga tcatcgcttc
    38641 tttgaccatt aaatccttga tattcatgat tatcctcctt gaaactaatc aatttttgtg
    38701 atttcaactt ctggcagcaa ctcatcaatc aattcgcgag ctgccaagtc atcagaaaaa
    38761 gctgttgcgc ttccgcaagc aactccccat ttaaaggctt cgattggatc tttcgtacgg
    38821 ctgtaagccc caataaagcc agcaatcatt gaatcccctg cacctactga atttttcaat
    38881 gggcgaacta atacattcgt tttataagtt ccttgttccg tgaagagcaa agcaccgtct
    38941 ccagccattg agatcagcac attctgtgcc ccttcagcta gtaatttttt cccatacgga
    39001 atgatttctt ctacagattg gaaggttgtg ttgtaaagct cagctaattc gtgattgttt
    39061 ggtttgatca acaacggttt cttaacaagt gcatttttta ggtcatcacc tgttgtatca
    39121 atcacgaaat ctgcaccttt ttctcgaatg atatcaatca gttcttgata aaatcctttt
    39181 cgcaggctag ctggcgcgct tcctgacaat acgacgatat ctccttgtcc tactgttcct
    39241 aatgcttgct tcaactcatc gatttcttca tctgaaatag atgggcctaa gccattgatc
    39301 tcagtttcag tttctgattt tagtttgata ttgattcttg tatcttcttt tacggttgtg
    39361 aacaaagact ggatttgttc tttttcaagc cacttcgaaa taaattcgcc agtgaaccct
    39421 cctaaaaacc ctagtgctgt agattgagaa tcgatccgtt tcaaaatacg ggaaacattg
    39481 atcccttttc ctccaggtaa cttgaagtct tccttcattc ggttcaaatc gcctagtttt
    39541 aacccatcta cgcgtacgat ataatcaatt gatggattaa gtgttactgt gtaaatcatt
    39601 gtattgcctc cttaattgtt gtcttttcac ggaattcatt aacgaattct aatggacaac
    39661 gctcggttat gatcgtggct gcttccaaag gtgcaacaga ggtgaaggac aactggttga
    39721 atttggtatg atcaatcaat acaaatgtct cttcggaatt tttcatggct aaacgtttaa
    39781 cagctgcctc ttctggatcc ggagtcgtga atccttgttc aagatgggca ccgttcattc
    39841 ccataaatgc tttattaaaa tgaaaatttt ttagttgttc tatcgcatta gatcctaaaa
    39901 cagcattagt tgccagcttt atcgttccac ctaaaataat ggtagcaagc tccaaatcaa
    39961 ctaaatttgc cgcatgttga acggaatttg tcactactgt gatctgtttt ccttgcagaa
    40021 aaggaatcat ttctaacgta gtagagccag cgtctaaata aatgacatct tcgtcattaa
    40081 tggtttcagc tgccgatttg gcgatttgct gtttttcgtg aacgtttttg agtgattttt
    40141 cactcatatt ctgctcaaaa ttcaataatt gctctttctt agctccgcca tgaactcttt
    40201 ctagaaaccc actattctcc agctcttgta aatcacgtct tatcgttgat tcagaagcat
    40261 tcaaaaggtt tacaagatct tgtgatttca cgattttctg ctgttcaagc aagtctagga
    40321 ttttttgtct gcgttcctct gtaagcattt tcacgatcac ctcaatacaa ataatagcat
    40381 attttttttc tactagcaat ataaatcagt caaaaacgtt cataaaaatt ataaatcagt
    40441 caaaaaaaaa taaaaaacca gtcagaaaga ctggtttttt atttttttgt ctatcatcaa
    40501 gccgttttat cgttttttat tttcgcttct tgtttttttc atataaacag aagcaggttt
    40561 tctttcctta tgaagaagat aataataaag tcccatcccg ccaaacatga ccaaaaaacc
    40621 gatgactgat cgaataggaa aattaggaac aaaaaagaga cagacggata ataatgatgc
    40681 cggaaccatt attcgataat agagttgttt atccattttc ttcacctcat attttttgta
    40741 aagtaatcaa ttgttctaat tctcggtaat ctaaaaattg cacacgcaga tgctgctcac
    40801 ctttcttgac atcgaattgg atacttcgcc gctttttctt tattcgtttg aaggtaacat
    40861 cagagagtct atgtttgatt tcctttaacg gtaattctga cagggattct gaaggaacat
    40921 agattcttaa ggataaaaga tactcttgat atttttcatc tgttacctct acttctagat
    40981 attccctaat ataataacaa taagcttcga ttccattatc caaagcaaca gtcggatcga
    41041 taactttata gcacatcatg cgtgatcgat tctcgtcaac ccaaggaaaa tacgcctgag
    41101 catttagtgt tccgcgaaca cgcctttctt tataatctcc ttgttggata tcataacttt
    41161 ttgttacaaa aaatgaagca ttctttttag gaacaggttg catttgtgca ttctgatact
    41221 ggacagattg gatcgtatgg tcagtgtaaa tgtattcaac gaatggcggt ttcaataaac
    41281 cgacaccatt gtcccaagca agatgatcaa acccaagctg gtccctaaaa taggattgaa
    41341 acatttgatt gtttggcttt tgccagttaa aggggtctcg ataaagctgg atacatatga
    41401 cttcgttttc taattccttc actttttcta cttgttggat gtcgagaaaa atcggtttag
    41461 ggatgatttg atgataatag cgactataat acatttccca acacgaagtg aaacataaac
    41521 cttggtaaaa aagatcgtat cctggaaatt ggttgcaatc gactactact tccccgtttt
    41581 gttgtttcat tttaggtaaa tgttcgattt catccttttt ctcaaacaat agccgttgag
    41641 agatctgttt tgtattgtga tagataaact cgctataagc tcgcatatag gcgaacaccc
    41701 catgagcaat catttttttc tgaatgaagt ctcgaatcaa aaattgcttt tctttgaaaa
    41761 cagagataga caatacattt tttatcaata attgattatc ttccaagcga aaactacatt
    41821 cgttctcgtc atttcctaca actaaccaac tttcgtctct taccgatgct agcattattt
    41881 tttcttgaag ctttcgaaga tcatacggat ccgcttgttg tgtgaaagag cggataaatg
    41941 ttggctggat caacagacgt gtcaaaatag cccaatcgct tatttcctgt tttttttctt
    42001 cttcatttaa gtctgtgagc tggatagtca tcaataattt atcttcgcca atcattttct
    42061 cacctctttc aaatcttttt ataatttaat tataaccaat gataaagaaa aactaaaact
    42121 ttatgtctta gaaaccaatt tggacgatta catgatagac taagtaaaaa gaaacaaaga
    42181 ggtgtttaca atggggttga aatttgaacg tacagataat ttagataaac ttttcgaatc
    42241 atttgctgtc gatccggaaa aaacagttgc accaaaagaa gagatcgatc gatttctaaa
    42301 accacagcca caaaatgaaa agaaaaaaga caagaaacaa aagtaagtta agctcaaaaa
    42361 aagacttacc agccaaactt ttatgaaaga
//
LOCUS       DASHRQ010000019        37762 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.019, whole genome shotgun sequence.
ACCESSION   DASHRQ010000019 DASHRQ010000000
VERSION     DASHRQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.019"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..122
                     /locus_tag="U2M88_001124"
     CDS             <1..122
                     /locus_tag="U2M88_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEM8373518.1"
                     /translation="HLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
     gene            complement(496..1065)
                     /locus_tag="U2M88_001125"
     CDS             complement(496..1065)
                     /locus_tag="U2M88_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEM8373519.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLYEL"
     gene            complement(1139..2418)
                     /locus_tag="U2M88_001126"
                     /pseudo
     CDS             complement(1139..2418)
                     /locus_tag="U2M88_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2772..3620
                     /locus_tag="U2M88_001127"
     CDS             2772..3620
                     /locus_tag="U2M88_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEM8373520.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3634..4707
                     /locus_tag="U2M88_001128"
     CDS             3634..4707
                     /locus_tag="U2M88_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEM8373521.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4697..6868
                     /locus_tag="U2M88_001129"
     CDS             4697..6868
                     /locus_tag="U2M88_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEM8373522.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6980..9151)
                     /gene="copB"
                     /locus_tag="U2M88_001130"
     CDS             complement(6980..9151)
                     /gene="copB"
                     /locus_tag="U2M88_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEM8373523.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9171..11357)
                     /locus_tag="U2M88_001131"
     CDS             complement(9171..11357)
                     /locus_tag="U2M88_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEM8373524.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11357..11566)
                     /gene="copZ"
                     /locus_tag="U2M88_001132"
     CDS             complement(11357..11566)
                     /gene="copZ"
                     /locus_tag="U2M88_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEM8373525.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11579..12019)
                     /locus_tag="U2M88_001133"
     CDS             complement(11579..12019)
                     /locus_tag="U2M88_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEM8373526.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12093..12566)
                     /locus_tag="U2M88_001134"
     CDS             complement(12093..12566)
                     /locus_tag="U2M88_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HEM8373527.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            complement(12876..14342)
                     /locus_tag="U2M88_001135"
     CDS             complement(12876..14342)
                     /locus_tag="U2M88_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8373528.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(14653..16737)
                     /gene="cadA"
                     /locus_tag="U2M88_001136"
     CDS             complement(14653..16737)
                     /gene="cadA"
                     /locus_tag="U2M88_001136"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEM8373529.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            complement(17261..17620)
                     /locus_tag="U2M88_001137"
     CDS             complement(17261..17620)
                     /locus_tag="U2M88_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEM8373530.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(17650..17991)
                     /locus_tag="U2M88_001138"
     CDS             complement(17650..17991)
                     /locus_tag="U2M88_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373531.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(17988..18662)
                     /locus_tag="U2M88_001139"
     CDS             complement(17988..18662)
                     /locus_tag="U2M88_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEM8373532.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            complement(19457..20755)
                     /gene="asnS"
                     /locus_tag="U2M88_001140"
     CDS             complement(19457..20755)
                     /gene="asnS"
                     /locus_tag="U2M88_001140"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEM8373533.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(20795..21985)
                     /locus_tag="U2M88_001141"
     CDS             complement(20795..21985)
                     /locus_tag="U2M88_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEM8373534.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(22006..22533)
                     /locus_tag="U2M88_001142"
     CDS             complement(22006..22533)
                     /locus_tag="U2M88_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEM8373535.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(22580..25369)
                     /locus_tag="U2M88_001143"
     CDS             complement(22580..25369)
                     /locus_tag="U2M88_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEM8373536.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDSSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAIYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKEIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(25516..25716)
                     /locus_tag="U2M88_001144"
     CDS             complement(25516..25716)
                     /locus_tag="U2M88_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEM8373537.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(26168..26488)
                     /locus_tag="U2M88_001145"
     CDS             complement(26168..26488)
                     /locus_tag="U2M88_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373538.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(26789..27906)
                     /locus_tag="U2M88_001146"
                     /pseudo
     CDS             complement(26789..27906)
                     /locus_tag="U2M88_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28377..29684
                     /locus_tag="U2M88_001147"
     CDS             28377..29684
                     /locus_tag="U2M88_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEM8373539.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFDNLF"
     gene            29803..31002
                     /locus_tag="U2M88_001148"
     CDS             29803..31002
                     /locus_tag="U2M88_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEM8373540.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(31029..32297)
                     /locus_tag="U2M88_001149"
     CDS             complement(31029..32297)
                     /locus_tag="U2M88_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEM8373541.1"
                     /translation="MEKQLPSPMRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLITLTL
                     FGTITTHLFGGSVGREGTAVQMGGSLADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(32460..33107)
                     /locus_tag="U2M88_001150"
     CDS             complement(32460..33107)
                     /locus_tag="U2M88_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HEM8373542.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILNHELPEIDTFLTTVLDVKLTKAQAERALEKLKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(33515..34018)
                     /locus_tag="U2M88_001151"
     CDS             complement(33515..34018)
                     /locus_tag="U2M88_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEM8373543.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(34141..34905)
                     /locus_tag="U2M88_001152"
     CDS             complement(34141..34905)
                     /locus_tag="U2M88_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEM8373544.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            35013..35288
                     /locus_tag="U2M88_001153"
     CDS             35013..35288
                     /locus_tag="U2M88_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEM8373545.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            35726..36676
                     /gene="yidC"
                     /locus_tag="U2M88_001154"
     CDS             35726..36676
                     /gene="yidC"
                     /locus_tag="U2M88_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEM8373546.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(36855..37055)
                     /locus_tag="U2M88_001155"
     CDS             complement(36855..37055)
                     /locus_tag="U2M88_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373547.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT        10822 a         7997 c         6658 g        12285 t
ORIGIN      
        1 accaccttcg gttgcacggt acacttggct acgagacacc ggttggttac cgtaaccaga
       61 gattggcgca gcgaatcctt gataatgagc tcggatgtgc taacgctagc gaggcagtct
      121 aactttaact gttagctcct gccgaagatc gtcacatccg aggaggctca tcgtcaagga
      181 caatcggaat agcatacgga aagaagttct gcaccttata aaatttgtca aaaaaactgt
      241 tgccattcca agtgaaaaaa agatcaatga atcaactaga aaactatata gaagagcaaa
      301 taaaaaagca ctcaaattga gtgctttttt aagctcacta tcactaaaac tatcactaat
      361 tttatttata agcgaacatg tttctatcac tgatttttta aatctagcga aaaaccctta
      421 tcttaaaggc gttcgttcaa ttctttggca aggtcttcaa agcctggctt gcctagaagt
      481 gcgaacatgg ttttcttata gctcatatag aaaaattttc ttgatgatga ttttatccat
      541 caattgagat tgactgtttt gctctcctat gatttcagct atgattgtcc gattttgcgg
      601 ttttcttctt aactggtagt tgattttccc agtttccatc aactcgctaa gcatatccac
      661 gactccgcca aatccggtct ttttcgtaat aaaacctagt gctaccattc gttcatctag
      721 attttttgct gtaatcgtca caccagtaat gagcgagtgc tgtaatgttt gggccagctc
      781 ttccatccct tcagcattgg atacgacgga agctgttttg ttaataggtt cttcgtttat
      841 ttcttttggt tctttcctct ccactttcac tgtttgacga aggacatcta gttcttgcaa
      901 gagcgtttcg tattgttctt gggtcagcat cacgccagct actttttcac gattaaatat
      961 atatacgcca gttcccgcgt ctcgcgcttg agcaaataca tccatcggcg atcgtttcac
     1021 ttctgtgatc gaagatgtgg gaacttctaa tttctttaat tccaatggat ttcctccttt
     1081 actaatcgct ttttattata gcacagactg acttgttttg atcttttttc caaaggtttt
     1141 atcggttttt gcctaaccag taattgatat cggtaagaag tctttcgata ccaaatgaag
     1201 gatggtacag atacgttttt tgatcttcgt tatggtctag tggaaaaaaa tgatcggtga
     1261 tcactagatc tgcataccga ggcggcttat ttcgagtaca tacatgtata gagatccgtt
     1321 ttcgatgtct tagcgttttg ttcagtaccc cagcaagtct tcttgcttct tggtgttcat
     1381 agcgaaaaaa aaagacgatt ttcacttcag gagggtggac atctaaatac cctcgttcct
     1441 ggagtgtttt cagtaaaagc atcccccact ctactgcggg gtgtgcaaat gcttgttgga
     1501 tcgctagccg aaattgatat agtttatctt tggaattggg aaatttttct aatgtttcct
     1561 gataataacg aacttcttct atcagccggt catggatacc aattacgtaa cgaagccatt
     1621 cttggtaaag tacttttgtc agctttgatt tgttttcgct ctcaaattcg ctattcagcc
     1681 aaaacagcga atcaagcttt tcagtcacaa tcgtcaattt ccgatagtct tgtgtctctt
     1741 ctatccgttt ccaccgcttt tcagtaatct ggttacagct caagcgcaaa attgctagat
     1801 aatttttttc ttttattggc aaaatctgct cttctctaaa tagcaattca ggaaacaatt
     1861 caaagaatag tgttcgactg tctcctaaaa tctcgatttc tgccggatcc tttctttgct
     1921 tcaataattc tcagaaaagg actgctctcc atcatctgca aaaaaatgcg gaaaatcttt
     1981 caaaaagcaa aacagcagat atcttacttt tctttctgaa ccaaagatct gtgctttttt
     2041 ataagaaaaa gaaaggtggt actcgccaaa ccactttctc aatcgctctt gcgtagaacg
     2101 atcgtacttc tgctcgtaag gttcattcaa tagcagattt ttcagtaaac aaaacggggg
     2161 tgattggatc agaaaaacaa acaagagata aggttcgcta tatccttgat cataaactaa
     2221 ctgaacggtt ttcttatgaa gaatcagctg gatgggacag tcgctttccg ccaaacgttt
     2281 cagatgccga tccactgttt taacatcgat tttcagttct tcagctaaat cagcttttcg
     2341 ccagatcaaa tctttcatta gattgattag tgaaaataaa ttctgtgcat tttttgataa
     2401 tagtttttgc catctcatcg atcatttctc acctgatttc taaacatatt gtttgatcaa
     2461 aatgggttca ctcctaaaaa gataaaaaac tccctttcta tttatttaaa tacgcaaatt
     2521 ttcctcatta tttttaattt tactcaattt aatcgtattc tcatttcaaa acgaatgtac
     2581 tttaaaaaaa cataataaaa tcactttgct tgtaggatgt attcccttcc attacaatga
     2641 aataaatagc aattttatga gaaggtgatg tgaaaatgga agaaaaaatc gaagaatcca
     2701 aacaaaaaat cgatcagttg atacaagaat tagaaaaagc ggtcatcggc aaacgttcaa
     2761 ctatccaatt gatgatcgtg gccctattag caggtggtca tgtcttattc gaagatattc
     2821 ccggtgtagg taaaacgtta ctggtcaaag cactagcaaa agctgtacag ggaaccttca
     2881 gcagagtcca atgtactcct gatctgctgc ctagtgatat tcttggtttt tccgtataca
     2941 attcacaatc aaaagagttt gaatttcgtc cgggaccgat ctttaccacg atcttgttgg
     3001 cagatgagat caatcgcacg actcctcgta ctcagtcagc acttttagaa gcaatggccg
     3061 agaatcatgc aacgatcgac aacaatacat accctctaga caatcatttt tttgtcttag
     3121 ctacgcaaaa tccaatcgag tatgaaggaa catatccttt gccggaagca cagctcgatc
     3181 gttttttatt ccgactacaa atcggttatc cttcttttga tgacgaactt ttgctacttt
     3241 tagataaatt cgaaaaagtg ttggataact taaatgaagt attgaacagt tatgaaatag
     3301 aagaaataaa aacaaatgtg gcgaatgtct ttgtcagtcc cgaagtggca tcttatgcct
     3361 tgcagttagt gtcagcaaca agaagccatg ctgccatcca gcttggcatc agtccacgag
     3421 gaagcctttc ctttattcag gcagcaaaag cttatgcttt aatccatggg agaaattacg
     3481 tcacgcccaa agatttgcag gatttagttc cttacgtgtt ttcccatcgc ctgatttttt
     3541 acgatcgttc attgaaggaa gacgaaaaaa aacaaatcat tcatactctt gttgcacaaa
     3601 ttcctgttcc tgtgaggtag attgaggtag ataatggaaa aaatcaaaga ttatttcatt
     3661 aattttgtgt tatttctggt atatttcatc attgtgcttt ttgctcttgt attcaacaat
     3721 tctttaggct ggttcttgtt ggcctttttt actctttatg tatcatggag cttgatttca
     3781 ttaagcagct ttctaaagaa actttcggtt acttgtacat tgcagccact cgtttatcgc
     3841 aaaaaaagta tgcaggttat tttttccata gcaaaaaaga gaactttttc tttacctctc
     3901 cctcgtttac aaatcggctt cccagcaatc tttgcagaaa aaaaacaaga gattttgatt
     3961 cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag aggatgccat
     4021 gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag aaaaagtgca
     4081 aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa
     4141 aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat
     4201 tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga ttggaaattg
     4261 tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga agaatgttct
     4321 cctctttttt gcttttgggg ttcgcacagt ccaaaatttg aacctctgct agacttgtat
     4381 ttttctttat ggcatttgag aaaagaaaag cagccggaac ttctgatatt aggcaaccgc
     4441 gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc tggcgatgag
     4501 aaagcttttt tcgcacacct aaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct
     4561 gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg aacggtttat
     4621 cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat atgttcattg
     4681 ccaggaggtg aatggattga cgattaaatg ggaaaaatgg ctgtgtggcg tttactcctt
     4741 tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc aaaacagcta
     4801 ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg
     4861 ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttgt attttccttc
     4921 gaagacattt tccttattat ggatcttaca gtttttttcg tcagtcacag aggaattctt
     4981 ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt ttctcataat
     5041 gttgctgatc tatttattta ttacattgac cgtggattac gactatttgt acgcaccctt
     5101 tctttctttg atggcttact tcatgatgct taccgttttt cgggaaaaag atttactgta
     5161 cgaaatgatt gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc aatttttctc
     5221 catgcggttg ttgtcgggaa acactcgatt ccagtcattg actacagttc tgcttatttg
     5281 tttcttaact attagcgcta tcttgccttt atatcttccg aaagtacacc aatctctcga
     5341 ggaaacgagt gaaccgatcc gagaatatct taatgatgaa gggctttatg atacactcca
     5401 tgagtatcaa ttaaccggaa gtgcaagaac aggcttcagt gagaatgatg aacagcttgg
     5461 tggaccttta tatgataatg gcgaagttgt ttttacagcg aaccaaaaag gaaatggcta
     5521 ttggaagatc gaaaataaaa atcattacac cggttctgga tggatagaag atcaggacgc
     5581 acccatccaa ccaatccaag aattccctgt tgacatcacg agctaccaaa ccgttatgga
     5641 agacaaagaa aacattacat tgaccctaaa aaaacaagct ttcttacctt atccttatgg
     5701 aaatactacc ttgactgatt tttcattagc tagtggacaa acgctttact atttgcctga
     5761 aagccagcga ttcattcctg tcccgaatga atcgacacgt tctgcaactt tacaaacaga
     5821 aaggttagtt tttacgagag aagaattaaa aagcagtcag ttaaaagctg atgatttaga
     5881 aaatcagcag acaaatcttc aattgccaaa aagtttgcct aaacgtattg aggacttggc
     5941 ggtcgaattg accaaaaatg aagcaacttt aattcgtaaa gtcgaagctg tcgaacagta
     6001 tttaaaaaca agcggaaatt tccgctattc caaaacggat accggacata ctccgaaaga
     6061 acacgattat gtggattatt tcctctttga ttcaaaagtc ggctattgtg acaacttttc
     6121 aacttctatg gtcgtccttc ttcgaacact tggtatccca gctcgctgga caaaaggttt
     6181 tacaaatggg ctcagaacag gcacagataa tcaagggaat gaggaataca gcattctcaa
     6241 tagtcatgct cattcttggc cagaggtata ctttcctgga tttggctggc ttccatttga
     6301 accaacccct tcatttgcca atccaatggg ttcacaacca gttgctgaaa caaccgaaac
     6361 cgtccagcct gctatcgaac aaccaaatga aagcgaaaat atggcagaaa ctatttccag
     6421 tgagagatct gaggtcattc catcagaaga aacaacactc acaccagctt ataaaatcgt
     6481 cttattcagc cttggggtgt gtttatacgt cttgttgttc tttctattgt tattcagaaa
     6541 atctctgcgc tggacaagtt atcttatcta tcttcatttt tttgcttcta ctaatacgat
     6601 tgcatatcaa aaacttctta accatttgga aacattggta ccgcgcaaac ccgcagaatc
     6661 tttgcaggaa tatagcctgc gaatagaaca agaattcaag ctagatctgc gtttcattcg
     6721 attaactact caatatgaag cagaaatata tggacgacaa gcagatccgg taaatttcga
     6781 taaaaaaacc tatttagaaa ttacgcatat aataagaaaa acggtataca aaaaagttct
     6841 gacaaaaggg agccgcttca aaaaataaga taaaatttct ggagaatcta cttctggaat
     6901 gccgagaacc agctgcttga gtatcgatta ttcgaaaaaa aggttgtgac acgttttttg
     6961 tcacaacctt ttttcattat tatttgattt tcaatgtcaa agcatttaga gctacaacta
     7021 ccgtacttaa cgacatcaag acagcaccaa ctgctggact taatatgata ccaattggcg
     7081 caagaattcc ggctgctagc ggaattgcaa gaatattgta acctgccccc caccagaggt
     7141 tctggatcat tttctttcgc gtttgcttgg ctagatcaag aaaatgcaaa atatcttttg
     7201 gatcgctgtt tgtcaatacg acatccgcag aatcgattgc gatatctgtc cctgcaccga
     7261 ttgccatccc gattgaagca cgggctaaac ttggcgcatc attgatccca tcacccacca
     7321 tgattacttt tttcccttgg tcaaggtatt tctggacaac agcttcttta tcatctggta
     7381 ataaaccgcc ataatattct tcgatcccta aataatctgc tactgcttgg gcagcgtttt
     7441 tattgtcacc agtaagcatg acaggagtga tttttctttc tttcaatgtt cggataaatt
     7501 cttttgcttc tgtttttact acgtcaccta aagcaacgat tgcagctaga tgattttcta
     7561 aaatcaggaa gctgatggta tttccttgtt cttggtaagg tttcaataat gcttgttcta
     7621 cgtttaatcc gagacgctca acttcttttt cattaactat tttcacttgt tggtttttga
     7681 ctgttgcctc tagcccaact cctgaaagat tttttagatt ttcggcttga taaggcttga
     7741 tttcatgttc tttcaaataa ttcataatac caacagccaa tggatgattg gcattcgctt
     7801 ccaatgctcc taaatattga aggatttcat ttttattgaa cgcatcatca agtacttcga
     7861 cacccgttac agtaaatttt ccttctgtta gtgttcctgt tttgtccagc atgatcacat
     7921 ctagatcatt ggcttgctcc agtgcattgc ggtttttcaa aagtaatccg tttttagctg
     7981 ctattgatgt agagcgagca acaactaatg ggatagctaa acctagagca tgaggacagg
     8041 cgatgataaa gactgttacc atacgttcta atgcattagg aagatctgtc aagaacaacc
     8101 aggcgatgaa tgccaggata cccacaatta atgcgacata aaacagccat tttgccactt
     8161 tgtcagataa tgactctagc ttggattttt ctccttgtgc ttttcgcacc atttccataa
     8221 ccttcgctag ataaccattt tcaccagttc cagttacttc gatttcgatc gttccatccc
     8281 cattgataga accgccaatc actttgtcat tggcttgttt tttgattcct cttgattcgc
     8341 cagtcacagc tgattcgtca acaattgttt cacctttcaa gattacacca tctgttggca
     8401 ttttatcacc agaccggacg atcaaacgat caccttcgtt tacttctttc aatgaaactg
     8461 tctcttcttt tccatctttc gtcaagcgtt ttactgattc tggcagtaat tctgctagtt
     8521 tttgtaaagc attgctggca ttcgacacag cattcatttc aatccagtga cctagtagca
     8581 taataacgat caaagtagcc aattcccaga agaaatccat gacgtgtgta tgtggattga
     8641 gcagatttgc gataaacgaa tagacactat aaatatatgc aactgtaatc cccatcgcga
     8701 tcagtgtcat catagcagga cttttttgtt tcaattccat cttagctcca cttaaaaatg
     8761 gctgaccgcc atagataaat agtatagttg ctaagatcaa tacgagccaa tcagaaccag
     8821 gaaacgttac ttgaaatgga aattccattc ccatcattgg tgaaaaaaga atgattggga
     8881 tagctaggat aagcgatagc caaaattttt gtttgaaatt tcccatgtgc atggaatgat
     8941 ccatgccgcc atgatcgtga tccatgtgca tcgaatggtc catttgtttg ctgtttgcta
     9001 tatctccatg gttatgtccc atgtgcatgg aatggtccat ctggctatgg tcatgtttca
     9061 tctccatctg tttttcatga gattctgtac tttctttgtt tactacttct tttttatgtt
     9121 catgtttttt ctccatatcg ttcccattca tttttactcc atcccttctc ttactttttc
     9181 atgtgccgat ttaagctcag ggagttcaac aatacactga cggaactaaa cgccatcgct
     9241 cctcctgcaa tgattggatt caaaaagcct agtgcggcaa aaggaatacc aatcgtatta
     9301 tagacaaaag cccagaaaag attttgtttg atttttctca atgtcaccct agacaactcg
     9361 attgtttgtg caatggatgc caaactgcta ctcatcagcg tcacatcggc tgtttccatc
     9421 gcgatatccg ttccacttcc cattgccata ccaacatcag ctaatgctaa agccggtgca
     9481 tcattgattc catcgcctgc cataccgact tttttgccat ctttctgaag tttttctacg
     9541 taggctgctt tatcctctgg aagtacttca gcaaagatat gctttggatc aataccaact
     9601 tgtttaccaa tcgtttcggc tgccagtttg ttgtctcctg tcagcataaa aacatctaat
     9661 cctttattct ggagctgctt gattgcttgt ttggcttcta gtttgatttg atcagccacg
     9721 gcaatcaacc cgatcacttg cttttcatca gctaaaaaca tgactgtttt tccctgctgt
     9781 tctaattcta acgcgtgttc ttggtattca tcaaatgata ggttcaattc aattaatcgt
     9841 ttacgtgtac ccgcaaaata gcgtactccg ttaatcgtac cgctgattcc agcacctggg
     9901 tgagctgtaa aatcaatcat atcgtatgtt gctgcttctt gcagttttcc gtattccacg
     9961 attgctttac ctagtggatg ttcagaagct tgttctaaag tataaaaaat agataggact
    10021 tctttagaac cgactagatc tgtaactttg ggctttcctt ctgtaatcgt cccagtttta
    10081 tccaaaacaa tgctgtctaa gtgagcagct gcttctaatg cttctccgcc tttaattaaa
    10141 attccatttc gtgcaccaac acccgttcct accataatag ctgttggagt cgctaaacct
    10201 aatgcacatg gacacgcaat gactaataca gagacactat ggatcaatgc gagttgccaa
    10261 tcaccagtga tcaaacctgt agctatgagc gtgataaacg cgatgactaa tacgatcgga
    10321 acaaaaatcc ctgagatccg atctgcgatt ttttggattg gggctttgct cccttgagca
    10381 tcttccacca tttgaatgat ttgcgctaat actgtctgct tgccgacttg tgtgacttcc
    10441 atgtgtagca gaccattcgt attgattgtc ccgccaaaaa gcgtgtcatc cggattcttt
    10501 tctacaggaa gattttcacc agtaagcata ctttcatcta tcgcactgct tcctgaaata
    10561 attcgaccat ctgctggaat ctgctcacca ggacggatga ctagttgatc accaacgact
    10621 acttcttcaa tcgctaacgt ttcttctttc ccgtttctta tcacttgggc ggtttttgtt
    10681 tgaagagaca tcaattgttt gattgcatta cctgttttcg tttttgctgt atgttccaaa
    10741 tatttgccga gcaaaatcag cgtgatgatc atactgctgc tttcaaaata aagatcttgg
    10801 gggtgtccac ggaaaaatcc gttgtaaatg ctcaatgcaa aggcagccga tgttccaatt
    10861 gctaccaata catccatatt tggtgctttc gacttgattg catggtacgc acctttataa
    10921 aaacgagcac cgacataaaa ttggacagga gcagacagca ctaactggac gattggaaaa
    10981 tgaaaaaagt gaacaatcgc cgcatggctt cctaacatca tagcaatcat tgacaacatc
    11041 aacggcaaag tcaaaaccgt actcaatata agatcgaata gcattttttt caaatacgct
    11101 tgcttttctt ctgcaatttt ttgtttgtgg gcttcatcgt acaaaattgc accatagccg
    11161 atgttttcta cgctttggat taatttttca gtggtcgtcc cttcatattt gacacttgct
    11221 ttttcagtgg ccaagttgac tgtggcattg accacaccag gttgtccgtt cagctctttt
    11281 tcgatcctag cagaacaatt tgcacaagtc atacctgtaa tgacgaacgt atccattttg
    11341 gtttctgctg ttgccatcaa atcacctctg ctttataacc caatccattg attgcttgac
    11401 aaatcttttc ggattgaact tttgtttcat caaacttgac cgtaccgttt gcttttttca
    11461 aatttacttt cactttttgc acgccgtcaa gtgcgcttac tgtttcttct actcttgctg
    11521 cacaatgatt gcagctcata ccttcgattg aaaattgttg tttcattttt tcttcctcct
    11581 attgatgttg tttacattca cattgacctg ggatacagtt acattcgatc gtatctactg
    11641 gttgcttttg ttccagttgt ttcaaaatct cattgatatc ttcttgagtc aaagtggctt
    11701 cttctactag atccgcaatc gtctctccaa tcctttttgc acagatatgg gaaaaaagat
    11761 tttccgtcgc actgcgaact gtttccatct ccgagacagc tggatgataa atgaattttt
    11821 ttccttcttg ttctgtccag atcacttctt tttttaccaa acgacccagc aaagttttga
    11881 tcgttgctgg tttccaatcc atcgaatcag ctaagatctg cgtgatttct tgggctgtcg
    11941 tctgtccaag tgtccaaata acacgcatga tctcccactc tgaatcacta atctttactg
    12001 gcgttttgac tttttccatt tatttcacac ctttcgttta caaatgtaat cattaactta
    12061 tgaatacaga ttacacttgt aaacaaaaac tgtcaattat ttttgctctc tattttcaat
    12121 ttagttaaaa attcttgttt ctctcgtctt gttttaagga tgatcagttt atttcttgca
    12181 ttagctactt ggatcagctg ctgtattttt ggttcattgt ttccttcaaa atgccagata
    12241 aaagataaaa attctaagta tttcctgttt agtttctccg aaaatgattc aggcatatct
    12301 ggccgagatt ttttgtttat ctttcttttt agagaacgcc aaactactcg gtagatggcg
    12361 agataacgcg gcactttaag ccaaatgatc ttatctgctt ctttaatcct ttcctctaac
    12421 gtagaagtat aatttccttc aacgatccat gacggattag ataccataaa cgattgctgt
    12481 tcttgcaaaa accacttttt tgcctgaatg cggaaagccg gtgatttctt tgatcttcct
    12541 tgcgagggac gattttcccg acccactttg tccaatcacg atgatcttca ttcttctccc
    12601 tcacttctct ttggaatttg gttcgatcat accacttata acgccgcaaa aaaacaaggg
    12661 actgtgacaa aagtcgtgtc aaagtccctt gtttcgaata aatggtgttc aatcagctga
    12721 ccttcagcat tagctcaaaa aacggcaaaa tatgaggagc tatttttgtt tggctgttct
    12781 tattacttga tgcaggacag ctttttcaca acctctgatt cacggtgttc cagaagtagc
    12841 ttctgtcaca acctcttgta acaagtattt ctattttaag gctgaaccac tgtttctggt
    12901 tcagtaattt ctttgttttt gtctcgacga gcaattgctg gcaagatcat acctaataag
    12961 atcaagacaa ttggtgtgat gatattcgtg aataattgga accaccatgc tttaggatca
    13021 gcagtataat ctacttttgg caccattcct aggatacatg caaacgcagt gaaggcaaag
    13081 caccaagcac caaagataaa accgattttt ggatttttaa caaatttata ttcagaattg
    13141 aatttcttat aagctttatt caacatcata tatgccaaga acacccatag gtaacgcatt
    13201 ggcatgacaa ctgagttcaa gttcgtcaac cattttacta attcattcat atctttgata
    13261 ccaaagatcg gcaacaaaat aatgattcct actagtatac ctgtcagaag ataaccattc
    13321 accaatgtac ctttacgatt acgtttacgt agccagtttg gtacaaattc tggatccgca
    13381 tctgctaaca agatttttag tggtgcatca attgaaaagg ctaatgcgga gatttgtcca
    13441 atcatgtttg tcaatgcgta aatgatcatc aatgagttac cgataccata gtaattacct
    13501 aaaatttgaa aagcactgta tgccccattt gccatcaagt ctttagggat attatcgcta
    13561 gcaaacagca tacccattgc gactgagcct aatacagcac aaatccctac cattgctgct
    13621 aagaagatca tccctttagg aaattccttt gcaggatttc ttgtagagtt gacataaggg
    13681 gatatttttt ccgcccctcc aactgcaaat actagcatcg aaacggttgt gaaataactg
    13741 aaatcaaatg ttggaatata tgttttcact tgattcatat gtggtgtggc aaattccatc
    13801 gttggattga tgaacggtgc cgttacagct aaaagaataa ataagatcga catgacgaac
    13861 atcgctgttc cggccagtcc gccaatcact ttcaaagtag aaagtccttt tgttgataaa
    13921 tataaaaagg caagaaatat aataagtgaa ataactgcta ccatttgtac tgacatcgta
    13981 tttacgatat ttccatttcc ttggactgcc caaccaccag caatcagaat tgcttgaggc
    14041 ttttgtgcca aataaggaat atggacgacc cagtaagtcc aagcggcata gtatgccagt
    14101 cgtttattag atgtcgtatt ttcgacccat gaactgacgc ctcctttact atccttgaag
    14161 gctgatccta attgtccaac gatcaatgcg tatggaataa aataaatggc caaaatcaat
    14221 agccaagagg tgacaactga tactccttgc tgagcatagt tattaactac attccccatg
    14281 ccccaaacca tgttaaatgc aattaacgca acagtaaacc agcgtaattg tttgttgttc
    14341 attcgttctc tcctttatga taaaaatttg ttatccagat gtaatcatat tcaattttta
    14401 acaaacttac aagacagtgt tattttatgt gtgtattctt ttatttaata cacatcgttt
    14461 tttcatattt tttccatcaa aaaaataagt tattttcttt tatttcattg accttattga
    14521 atttcaagct attttgaatt ttctgcaaaa ttaaaaacat tagttatttt ataatcctat
    14581 ctattttctt tacatttttc attaagaaaa ggatataaaa aaaagaggac gttcattcgt
    14641 cctcttcatt gatcaacgtt tattttttac atgcaagctt cgcatggcat tcaaaacagc
    14701 taatactgtc accccaacat ctgcaaatac tgcatctccc attgatgcaa aaccaaaagc
    14761 acctaaggaa agtaccaaaa tttttatccc aatagcaaag acaatgttct gtttgacgat
    14821 tttcaatgtt ttctttgcta aatggattgc ttctgcaatc ttcgatggtt gatcattcat
    14881 gatgactaca tctgctgctt caattgcagc atcgcttccc aatccaccca ttgcgattcc
    14941 tacatctgct cttgctagca ccggtgcatc gttgattcca tctccgacaa atgcggtctt
    15001 tttattgtta ctttgtaaga tttcctctaa ccgctgcact ttatcttctg gaagcaattc
    15061 gctatatatc ttgtccacgc ctacttgctt cccgatatga tcagcaattt ttttcgagtc
    15121 gcccgtcaac atgactgtgt ttttcacacc agcctgtttt aattgaacca atgcgtctac
    15181 ggcatcttct ttcaaggtat ctgcaataac aagataacca ctgtaacttt gatcgatcgc
    15241 tagataaagc aatgtaccga tttcttgact tgaaggaaaa gagatttcaa atctttccat
    15301 caatttgtga ttacctacta atacttgttt gccatcaact tcagctaaga tcccttcacc
    15361 tgcaaattcc tggatattag ctgttggtaa aagcggacct gcgtagcctt ctaaaacaga
    15421 ttgagcaatc ggatgtgtag aaaactgttc tgcacttgca acatattgca taaatttctt
    15481 tggatcaatt cctgttggat cgatcgtctg gatcgaaaag tcacctttcg ttaatgtgcc
    15541 agttttatcg aataccactg tctcagtttg tgcaagcaac tccaagtaat ttgaaccttt
    15601 tactaataca ccgattttac ttgctcctcc gataccgcca aagaaactaa gtggcacgga
    15661 aatcactaat gcacatggac atgagatcac taaaaatgtc aatgctcgat agatccattc
    15721 agaaaacgcc gctctagaaa caacaagtgg aggtaaaaca gctaataaaa cggccaatcc
    15781 tacaacgact ggtgtgtaat agcgcgcgaa tttagtaatg aaattttcag ctggtgcttt
    15841 tttactgctg gcattttcta ctaattctaa taatttgctg atagtagaat tttcataaga
    15901 tttttctact tgaactaaaa atggtttatt tttattgata aatccgctta atactgtttc
    15961 accaacatat acagatttag ggactgattc tcctgtaagt gctgacgtat cgactaatga
    16021 ttctccttcg atgatcgttc catctaacgg tacacgttca ccaggcttca caagtacaaa
    16081 ctgaccgatc agcacgtctt cagggttcac ggtcatcaat ccttccgccg tctgcacgtg
    16141 agctgtttct ggacggatcg ccatcaattc gcgaatcgat tttcttgaat gactgactgc
    16201 caatccttgg aaatactcgc cgatttgata aaatagcatc actgctacgg cttctggata
    16261 ttcaccgatc aacatagcgc caatcgtagc aacagacata agaaaattct catcaaagat
    16321 ttttcctttg acgatattca tcactgcctt cttcactaca tcatatccta tcagcagata
    16381 aatcaataaa taccctatca agcgaattgg ttcattaaaa tcaaataata gcaataaagc
    16441 gatacctgca agggaaaata aaatccggaa cagcgtattt ctcttttttt catttccatg
    16501 ttcagatact ttttccttat ctatttctgt tactttgaca tctggttcta attccttcac
    16561 tacttttttt gtttcttgtt caatcgtagc caaatgatct tcctctgcgt caatcgttag
    16621 ttttccagaa gtgaagttta ccgttgcttc cttgactcca ttgattttct gaactccttt
    16681 ttcgattttc attgcacagt tcgcacaatc taaaccttct aatctgtaac ttttcatttt
    16741 aatgacctcc tcgcctcaac atatgaatat ctcttcataa ctaattataa atgaatggtt
    16801 attcatatgt caaggtatcc gaaagaaaaa atgaaaaaca tctgcatcgg aaaatttctt
    16861 ccatacagat gttttggctt attctttctt tttctcttcg atcattgcca ctgctcttat
    16921 ccaacccgca cttgccttct tgatcttgac aggatagcaa ctaagtttga agcctgttgc
    16981 tggcaatttg tctaaattca ttaatttttc gataggacaa tatctttttt caatccctgc
    17041 aaaatgtcct tcccagatat acgaagctcc actacgcttt caaaaatata gaaagagttc
    17101 tttaatgaaa attatttcaa ctttccataa acagtaaccc catataaata aaagacgtaa
    17161 aagtcacata gaagaactta tacaggaaaa cgcatagagt caggtgaaat acatgtatcc
    17221 cacctggctc tatgctttat ctaaccttta ttattctctt ttatacaatt tctgcaccga
    17281 atggcatcaa cgccaatcga gcaagtttga aagattgctt ggcaaaaggt atccctatga
    17341 tcgtgataca tagaaggatg ccgctgacga catgtgcgag agcaattggt aggcctgaaa
    17401 cgatcagcca gatgatatta acgatcaatg acatgccaga ctggtgataa attacttctt
    17461 tgccaaaagg cgcaaaagaa agggaggcga tcttaaaaca ttgcataccg attgggatac
    17521 ctatgatcgt gatacaccaa agtatccccg ctgctaacca gctaagacca ccgattagtc
    17581 cgccgaatat aaaccagatg acatttccta aacaactcat tgtatttctc cttctattac
    17641 tgtttttgat taaaactggg caaaaattga ttttattctt ctttttttag cttccagcat
    17701 caagtattct gcaaattttt tttggatttc ttccgtattt tcttttgtta ctaatgtgtg
    17761 ttggattagt tttcctgatg gtcgctgatc caatcgttct tctgactgtt gcacgtccgc
    17821 ttgcgcaaat ccgataatcc gtttttcttt tgttacagca acgagcaata gtttattctt
    17881 atcatttagg tcagctgccg tttttgcttt tacgaatgat tctggatgat cagtaatcag
    17941 ctgattgatt tgaggtaaat cttcttttac tgcccacctt atttccatca tttttcactt
    18001 ttctttcgct catttttatc tccactaaaa aaatcgactg cgacaattgc atctatccag
    18061 ctttgtgcaa tgatctcatg tccgccaatc gttgggtgga ccccatcttc tcttgtatat
    18121 ttcgaaaaac cataagcaat ccctaaagca ttgaatatac catctaaagg aaccagcaat
    18181 gcgccatatt ccttagcaag tttacgaaca atttggattt tagggtctaa gtctttacgc
    18241 cagccatgac ggtcgtcggt tataggaagt acaaacggct ccatcaagat gatgttgcag
    18301 atttccgctg tttgaagttc atttagtaat tttcgataaa cggattcaaa ttgctgagca
    18361 gatttctcag ttccgaatat agcctgatcg ctagtattat gccaggtatc attgattccg
    18421 atcaaaatcg atacatagtc aggttgtaaa gctaaacaat cttcttgcca gcgtacttgc
    18481 agatctgcaa ttttgtctcc tcctatccca cggttcatgc attcaaattc catcgttgga
    18541 tattgggaga ttagttttcc ggcaatcaaa agtggataac ctttacctag atcagttgct
    18601 tctgctcggt atcttcctgc gtctgtaatg ctatcaccga taaataatat acgttttttc
    18661 atttgtcaaa aactcctttt tgaataaaac gatgattttc ttccttatat tatcccataa
    18721 gccctatcaa gacgaaagag aaaatatatg tataaaaaat gacaccagcc tagttccttc
    18781 aaacaattga atgaacgctc atcaagtgtc tagtttttta tgctattttt ttcttaacgt
    18841 gccggattcc ccttgattcc ttcgttgttt ggtccagaga gatcgatcac acccaagctt
    18901 ccgtcaatca gaccactgat gacgccatcg aaacatatat ttataggctc atctaagact
    18961 tctagtaaat gtccctttac taatatctct tctagattgc ttgggataga ttggtcataa
    19021 ctttatctgg tccgatgatt tcaagggtga attgaactta catatggatt attgcatgct
    19081 gaaaatacgg acctactcca tcttcgaatg ttactattag tttgttttca ggtgctagat
    19141 gctgctttat ttcaacagca gaagcatcat caaatcagat ttccatttga attaccttct
    19201 ttgtaaatgt ttgattgatt gcgatttcgt tatagaacct tgtgaatcaa atagcaattt
    19261 cttttgcttt cttctcttgt ctgctggaaa cttccattat cccgtattta aaaaaaagaa
    19321 ctgcgagagt tcgcagttct tttttttaaa taaacgttgc tctccggcca caagtcacaa
    19381 tagtcatcaa acatgaaaaa ctgttttttg aaattactcc ttgtgactcg gagctgaacg
    19441 gcaggcgcac aacctcttat ggataaattc ggttcaacaa acgtgggaat ggaatagctt
    19501 cacgtacatg gtcgatacca gcaatccaag tgactgttcg ttcaagacct agaccaaaac
    19561 cagagtgcgg aacagaaccg tatttacgta gatcaagata ccatgaatat tctttttcat
    19621 ccaaaccgtg attacggatt tgttctagca agaaatcata gtccgttgcc cgttcgcttc
    19681 cgccaataat ttcaccgtaa ccttctggtg cgatcatatc tgcacaaatt accacgtctt
    19741 ctcttgttgg atgtggtttc atgtagaatg gcttgatggc ttttggatag tttaggataa
    19801 agacaggctg ttcgaatgaa ttcgcaatga acgtttcatg aggtgaacca aagtcttcgc
    19861 cccattcgat gtcatcaaat ccatttttct tcagtaattc gattgcatca tcgtaagtaa
    19921 tacgagggaa tggcaatttt gtataagttt ttaagacttc tttgtctcgt cctagtacat
    19981 ctaatgcgta ttcgcaatga tctaaaacat tttgtactaa gaatgctaca tattgttcct
    20041 gtacttctaa actttcttct tgatgagtga aagccatttc tggttcgatc atccagaatt
    20101 cgatcaaatg acgtcgtgtt tttgattttt cagcacggaa agtcggaccg aatgaaaaga
    20161 cttttccgaa agccatagca gcagcttcca tgtataattg gccactttga gataaataag
    20221 ctttttgatc aaagtaattt gtttcgaata gatccgtagt gccttctggt gcagaaccag
    20281 tcaagatcgg cggatcaatt ttgacaaaat gatgttgatt aaagaattca tacgttgcac
    20341 gaatgatttc atttctgatc tgcataatcg catgttgtct tgaagaacgc agccataaat
    20401 gacgatgatc catcaagaag tctgttccgt gttcttttgg tgtgatcgga taatcgtggc
    20461 tctcgcttac cacttccaca tttgtaattc cgatttcata accaaatttt gaacgactgt
    20521 cttcacggat ttctccagtt actaacacgg atgtttcttg gtgtagattt ttagctgttt
    20581 ggaaaacttc ttccgacact tcgcttttta caactacacc ttggaaaaag gctgttccgt
    20641 ctcttaattg taaaaatgtg atttttccgc ttgatcgctt atttgcgacc caagcgccga
    20701 ttgttactgt ttctcccaca tggtttttcg aatcgatgat ttgaatttgt tccacgtatg
    20761 actctccttt tatattcctt gaatttcttc aggattaatt ttgactttta ctctcaataa
    20821 attgcttgat tctgcgtacc gcttcttcta acgtagcgcg atcagtagca taactcatgc
    20881 ggacattttc tggtgcccca aagccttcgc cagtcacaag cgctacccct gtttcttcta
    20941 aaagatcttc tacccattgt gtgacatttt catatttaca catgcgtagc gtttctttta
    21001 cattaggaaa aagatagaaa gctccttgcg gcttttcaag agaaacacct ggcagttctg
    21061 ccacaagcgg atataacgta ttcaggcgtt cttcaaaggc gttccgcatt tcttctactg
    21121 tatcttgctc acctttcaat gcttcaacag ctgcatattg actgactgct gtcggattac
    21181 tggttgactg ggaagcaaca gcaatcattc cattgatgat ctcagaattc cccaccgcat
    21241 aaccaatgcg ccagcctgtc atcgcatatg ttttagacac gccattgatg atgatggtct
    21301 gatttttgat tgcttctgaa atcgttgcaa ttggagtaaa ctcattgcca ttataaacta
    21361 aacgaccata aatatcatca gcaactatca aaatatcatt ttttactgcc cattcaccga
    21421 ttgcctgaag ttcattcttg ctataaatca ttccagtcgg attcgatgga gaattgatga
    21481 ttacggcttt tgttttttct gaacgtgctt gttctagctg ttcaacagta actttccagc
    21541 tattggattc ttcccctttc acaaagacag gaagtccttc tgccagtttt acttgctcac
    21601 cgtaactgac ccagtaaggc acaggaatga tgacttcatc ttgcgggtca aggatcgttt
    21661 ggaaaagggt atacaaagca aatttcgctc cgtctgttac gactgtttgt gaaggttcat
    21721 agacaagtcc atagtttttc ttcagataat caacgattgc ctgtcgaagc tctgggattc
    21781 ctgctgttgg tgtataatag ctagctttcc cattgcggat tgcttcgatt gctgcttctt
    21841 ggatgttttc tggtgtcgca aaatccggtt ctcccacagt caaactcagc acatctctgc
    21901 cttttgcttt taatgccttg gcctttgccg cagctgccaa agtgacagac ggttctaatc
    21961 gttttgttct ttttgataac tccatcccat tcattccttt accttttaaa ttcccttgat
    22021 tgtcttgact tcatctccag attcaaagcc aatcaggtaa taattcagtt tcccatttgt
    22081 ttcttttcct actacttccc acacaggctg atcatcatac atccctaaag cagctttttc
    22141 gatttggatt tcaggatggg ccgcagcaat tgtctgtttt gcttcgtttt cagtcagtcc
    22201 ctttgtttga tccaatactt ttactttttc accagattta ggaatgataa cggcgatttc
    22261 ttttccttta tcatttgtac ctgtcagtga aaagtatgtc ttctttcgag taaaccaata
    22321 aaaatgatcg gtttcctcaa gatttgcata tttctttgcg atttctactg cttctttctt
    22381 cgcctgcacc gccggacgac tggaacgcaa gtaaaaaatc gagctgatca cgatgaccgc
    22441 taataaaaat acgatcaagc caagtaacac gcgattaatc gttttttcct ttgtctgttt
    22501 tttcttcaag ggtcgtcgct cctttatctt cattcctttt cattatagca gattttaatt
    22561 atgaaaaaag acagctcctc taacttcctt cttcattttt ctttaagaat tcatggatat
    22621 cctgatagat ttcctccata gctagctctt tgatcggtac ttttttcggt aatgcatgga
    22681 tgatccgtgc gccatatttt gcagtgacta gtcttcgatc caaaataagc aatacgccac
    22741 gatctgtctc cgtccttatc aagcgaccta atgcttggcg taacttcaaa gctgctttag
    22801 gtacggattc ctggtaaaaa ggattgatcc cctctgattc taaataagcg ttcctagctt
    22861 tgaccatcgg gcgaagcgga ttatcgaagg gtaaacgtgt gactacaacg atctgcaaag
    22921 catctccagg caaatctaca ccttcccaaa agctgtcagc gccaaacaat acgctttttt
    22981 ctgacaaaat gaagcgtttc aaaagcttct cccggcttcc accaaaccct tgagctagta
    23041 tttctcgtcc tttttctagc atagacagat gcattttctg atagaccttc tgcaaaacat
    23101 catgagaagt gaataacaca agtattggcc gatcttcttg ttcgatcaga cctgtaagtg
    23161 ctgtctctaa ataatcgatg tatgcttgat tgctgacttg atggacggca ggtgcctctt
    23221 ttggcacata gatccttgcc tgtttctcat aatcataagg agaggcaatc actttaagag
    23281 gtgctgcttc aatgccaagc ctcttagaga gatagtgtcg attagggccg atcttcagcg
    23341 ttcctccaat ataaagaata cgatcatatc gaggatacca aacagtcgac ggcaacatag
    23401 ccgcttggaa atcatggaga tgaaaaatca tctgctgcgc tgtgttccca ggaacgaacc
    23461 aatggatcaa atgatctcgc cagtcttcca gccattgatc catcagcaag tactgcttgt
    23521 ctaattgatc aaataagcta tataaacttg ctaaatcgat ccgcatctgg tttgtccagc
    23581 tttcttggat aatctcaaaa gtcagacgca tttgtgactg tagctgctga atctcctgat
    23641 aaaaaagctg caggtgatca atggctgtcc cagattccag tggcagctga tccagcatct
    23701 cttttgtcat catgacttct tctggatact gtccgttggg ttgcttgccg ctcactaaac
    23761 tcattaatcc ttctgccata caaatctgtg cttcgcatag cgcagaaagt tcttctcgat
    23821 aaatcgataa ccagcgtaag gcttcatgtt ctttcggtaa atagctttgt attcggtcaa
    23881 acagctgatt tggttcttct aattgctgga tttgtttatg gaaatagtta atacttaatc
    23941 gtttgtcatt tactttttca gcgatttccg gcaaatgatg tacttcatcg atcaacaaaa
    24001 atggactagc tggaagcaat ggttcttttc gctgtgtttc ttgtactaaa aacgcatgat
    24061 tgacaatcaa aaaattactt tgcctgattt tcttttgcaa gaaacgaaca aagtcttgtt
    24121 cgtaaaacga ctgatttctt gctaaaaacc cagtacctct gtgcgcgata tctgcaaaga
    24181 atgcatggtt caagtttgtt aaatgtaatt catccaaatc accggtcttc gtttgcgtca
    24241 gccaaactaa gaccgccatt tgatacaaag catattgttt ttgttctggt ggttccacaa
    24301 gtgttccttt aaatcgttgc aggtcgatat agtggcgata acttttgata attacaggtt
    24361 gtatgggctg atctagcaat tgattcagca atggaatttc tttttctatg atctgctgct
    24421 gcaaaactag cgaaacggta ctgatgacag cgggtttttc aggtgtagcc aaataactca
    24481 atggcaacag ataaccgatt gttttaccca ttcctgtagc tgcttcgatc atcaaatctt
    24541 ttgattcatc ttttgtgaaa tggtcgtaga tcgcattcat cagtctgttc tgttcttttc
    24601 gatacatcag tgttttacca aacatctttt cttttgcttg tttttttctt ggatatgtcc
    24661 gctcgccgta atggacggat gtgaatagtt ccacttcttt tttctgcaaa gctagcccat
    24721 cgacgatttc taatgaggaa tcaagtggct ctggtttttc tttcatttct tcaatcacat
    24781 gataaatgaa acgactcgta tccatacctg tgtgtctact taatcgcgca atctgctcta
    24841 acgtgatgat tggcaatttt ctcatccgtt cttcgatcag caaaagaagt tgacctgtca
    24901 cttcggcatc actatctgcc tgatgaggat tctcatgaat aaatccaagg ctttctgaca
    24961 aatcagctaa gcgaaagctt ggttccgtcg gcaagaaaat ttgtgcaagt tctactgtat
    25021 cgatgcctgg tatcttcaaa ctaggaagtc cgcatcgcat gagttcatgg ttcaaaaaat
    25081 tataatcaaa ataaatatta tgcgcgacaa atatggtgtc tgccaaaaga ttgtaaatcg
    25141 tttgggcgac atcttcgaaa taaggcgctt tttggacgcg ttgattcgtg atccctgtta
    25201 aatgctggat ttgttttgaa atcttccgcc ctggatgaat atcgatcgaa aaacgtgaag
    25261 tgatccgacc tgattcaatc atgacgcagc caaattggat gatccgatct tcttttggat
    25321 tcgtcccagt cgtttcgata tcaacgactg cataagttct tggtttcaaa atatcttcct
    25381 cttctcctgc ctctaatgtt cttttgaaat tatactacaa agtgcagaaa aatactctca
    25441 tcgcttttta aagaaaatag acaaaaacgc ccagacacgc aaatgtctgg gcgtttattt
    25501 tttagtatag gttaattaag ctttagtaac gtttgttgct tgaggtccgc gttggccttc
    25561 ttcaacatcg aaagttactg cttggccttc ttctaaagtt ttgaatccgt cgccttggat
    25621 agctgagaaa tgaacgaata catcgtttcc gttttctgca gtgatgaatc caaatccttt
    25681 ttctgagtta aaccatttta ctgtacctgt ttccataata aaaatcctcc ttgtgtatca
    25741 acacatattt tgcaattact tgaatggtgt atatctggaa gatgtttatt tgaaacaagc
    25801 taccacagat taccgaacaa aaactactcc tatagtatat cataccgttt acaaaaaagc
    25861 tagagttttt cgactttttt tattaaaaaa agttttctca gcgattacat aaagtgaaaa
    25921 aacgaacttt ttttaaaata aacgacatat tgttcagttg gtgttttttt gctctgatgt
    25981 tcttctttaa actaaaaaaa ttattctttc tgactactta tcggttctgt ttcaacggtc
    26041 gaggctgggg aagattcttt agtaggtctg atgttctcat ggtatttctg atagcctatg
    26101 acagtgacca gaagaacaag gcaaaccaat acgatcaacg agattagttt ttcttttttg
    26161 ctcatatcta tacctcataa ataacaggat gatgtccatc atatttctga gtatgagtaa
    26221 aagcaaaagc tgcagctgta gaatgtaaga aatgccagca actttcataa tcacaggtcg
    26281 gacagtacca aaagtaacca aatcttggct gcaaagaccg ttcttctgtt tcaggaatcg
    26341 cagaatcaag atctgttgtc tgtacataaa catttttgta cagacgtgtg cctgtaatag
    26401 gagaccgctg tgtttcttca gcaaaaactg gctgacttgt taaacttagg gatagaaaca
    26461 gcaaacacca taacagtctt tttttcataa agacctcctt atcgataagt ttttcccaat
    26521 taaaatcata tctcaagaac gcccgttatt cacattttgt tcataaatga tgttttttgt
    26581 atcataatcg tcaaaaagag gtcgtgacca cgtcacaacc tcttttttaa acggtgttcc
    26641 agaagtagct tcttcggaaa taagccgaaa tttcacaaaa atttgagaaa taatttttgt
    26701 caatctcttc ttatttcttg aagctgacca cttctgtcac aacctcttcc ttaatactca
    26761 ggtcaaaaca ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt
    26821 catctggcgc atctttttga tccaaaataa acactttccc gccattaatg atcacatcat
    26881 ctgctaatgc attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac
    26941 catcagctaa gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg
    27001 gaacgatatc aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg
    27061 attcttgagc tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg
    27121 atagctgcgc aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa
    27181 ttttttgaac atcgtctgat tttctggtaa tgcaaataaa taaatcggcc attgttcagg
    27241 attttcgaaa gaatccttta agtacgtatc aacagcttgg taatagttga cccaatcgat
    27301 ctctacttct tcatcttttg gatttacgcc atggaaagtt actccatctc gggctccgcc
    27361 tcctccatta gaagagaagt tcaaaccgcc acctgtcagt tcagatccca atgcagtaac
    27421 gacatcaacc ggtgcccctt ctggtagaga tacttctttt acttgtttgt tgctgacctc
    27481 atacaatttc atggagtcgc ggttcaatcc caataaataa taagaatagt tgaactgccc
    27541 atttttgata atagccagta aataaggaag atcacttaca tagtattgat cgtctaccgg
    27601 gatactcaaa cgatggatat aggtctggtc agcagttata atgaccgcta cactcgtagt
    27661 tgcactacgc caaaacgaag gatctcctaa caaattttca attttttctt ggaacggaga
    27721 ccagtcatgt tcggaatatt ttttctcaaa acgtttcttt gcttctttcg caaaattttt
    27781 gaatttgatc tgatcttttt ccacatcttg atgggcgaca tgcgtattca gcatgatcgt
    27841 gacgaatggt cctttagcgc cttctgaagt caattgtgtc aataattctt tatccttttt
    27901 tgccataaac gtacccctcc taaaaaaatc attcctttgt ttctatctaa agtctaccac
    27961 aacactattt ttccatacaa taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt
    28021 aaatttagct atttttttaa gttatcttca taatttcctc aattatttag ccccagtctt
    28081 caactattct tcatgaaatg ctgataaact aagaaaaaga ataaaagagg tggatcttat
    28141 gcatttaatt atgattttca gtttattaag tattatcagt ttatttgctt tcagcgcttc
    28201 ccgcttatta gatgcggaaa cacggcctat tcctgtaaaa gtacgtaaaa acgaccgcta
    28261 aacttccata acgcgcgttc ttatcctaaa gttttttcag aaacattgaa actctctccc
    28321 ctctctgata aaattttaat ttgtagcgaa acaaattaat tacaaggaga tcggatatgt
    28381 ccataaaaaa aacacgtcta gtcctattat tggctagtct cattctttgc cttgcttctt
    28441 ttctaccagc tgttgctgtg caagctgaag atacgttcaa agtaaatgca aaagcagctt
    28501 ttgctgtaga tgcgcaaagc ggtaagattc tttatgatca agatggagaa aaaaccatgg
    28561 ggatcgcttc aatcacaaaa atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag
    28621 gaaaactgtc atgggatgat aaagtttcga tttcagatta tgcagaaaat ttgagtatca
    28681 ccccagattt atcgaatgtt cctttgcata aagaaaacac ctacactgtc aaagaattat
    28741 tcgactctgc aatcatccag tcagccaatg cttcaatggt ggccttggca gaaaaaatct
    28801 ctggcagcga agctaaattc actgaacgaa tgaaagaaca attgaaagat tggggaatca
    28861 aagatgcaac aatcgttaat gcatcaggat tgaacaactc ttatcttggc gaaaaccgac
    28921 cagaaggcac tggagaaaac gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg
    28981 cccgccattt gatcttggat ttcccagaaa tactggatgt atcaagtact acgacccaaa
    29041 tgtttggcga aaatacgcaa tcaccagtgg aaatggtcaa ctggaactgg atgctcccag
    29101 gattcgttaa ttacaaggaa ggcgtggacg gattaaaaac tggtacgact gagcttgcag
    29161 gtgcttgttt cgttggtacg atcactaaaa acgatcaacg gatcatcact gtcgtattga
    29221 atgcggacgg ccatgcggaa aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg
    29281 actactgcta tgacaattgg tcagaaaaag agctcggaaa agctaacgca agtattccta
    29341 gtctaaaaac cattgatgta aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa
    29401 gccctgtaaa agtatgggta cgaaacgata tggatactgg caaattaacg atcacgccaa
    29461 cacttgacaa gaagcaagtc actgacaatg agatccaagc acctatggat aaaggtacaa
    29521 aaatcggtac cgctacgatc accttggcag acgataaatt aggctattta gaagacaaca
    29581 atgcaccatc aacagagatc attacggata aaacagtgga aaaagcgaat atattcgtga
    29641 ttggctggcg acgcgtcgca gaatttttcg acaacttgtt ttaattgaac aataagaaaa
    29701 acatctttcc atcggaaaga tgtttttttg ctttttctag tttaaataac agcttttttc
    29761 atctattttc gttatactgt ataagaaaaa ggagttgaat ttatgaatct aaccagagat
    29821 ctgatctttt tagttctcta tcttattggg gtattctcta tcgtttattg gcgaaaacag
    29881 aagaaggatc cttccttgtt cttttacggc agtacgtgga catttacggt aatcttaact
    29941 tattttgtcg tgttggaaat tttttattcg accttttatt ttttgattcc attgttgttc
    30001 tttagtatct ttgctttttc ttatttcaca gaaaaacgaa ggctgctgaa tggtctttta
    30061 ttcaatgttt tcctcatcag tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa
    30121 aatattttcc ttggcggact gatcgccttg atcacgattc cattgctact tgtcctttta
    30181 tttggtattt atggattgat tgtttttctt ttttggaacg gggtcacagt tttacgtaga
    30241 gaatcgcatt cattagctaa tcttttaacg ttgatactag ccatttttct tacactgttt
    30301 cttgtttttg actttttctt gttaaaatat ttgccgcaat ggataaatgc tttgtttttc
    30361 tgcgttcccc tgatcttgat ctatctattt atcgtattct acaacttttt gactgtctca
    30421 ttcttgtatc agttcaaccg gcctcgatac aatcaagact tcatcgttgt gttaggcgct
    30481 gggttgatca atggagaaac agtttctcct cttctagcca agcgaatcaa taaggcaatt
    30541 gctttttatc gggctcaaag ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc
    30601 gggcaaggag cagacgaaaa agtaccagag gccattgcga tgaaacaata tgccatggaa
    30661 caaggaatcc ctgaaagaga cattcttgtc gaaaccaatt cgacaaccac tttggagaac
    30721 atgctctatt caaaggaaat catggatcag caaatgaaag ggccataccg cgcgatcttt
    30781 agttccaaca attaccacat ttttagagcc ggtctttatg cgtggcaagc caaattgaaa
    30841 gcaaatggta ttggtgcaaa aacagctttc tactacttgc ctaatgcgtt tttaagagaa
    30901 tacatcgcca tcttgatgat gaacaaaaaa agacatatga tcatttgcgg attgcttttc
    30961 gcaggctccg cattcttatc gctgattact ttacttttct aaaaaaacaa ggtgtgacaa
    31021 aacatatgtc acaccttgtt tttttctttc attctcttaa ctattggcaa aaagagtatc
    31081 ccttttttga ccgctacttg ctgggcagaa taaataccgc tgttgcctga acacataaag
    31141 ctgatcaagc agatcataaa gaagtatact gccgcttgtg aaccgaacaa ttcgatcccc
    31201 atcacaaagc aggcaatcgg tgtatttgtc gcaccagaaa atactccgat aaagcccaat
    31261 cctgcaagaa aaggaacaga aacatgaagc aaaggcgcta gtgctgcacc aagcgttgca
    31321 ccaatttcaa acaatggtgt cacttctccc ccttgatatc ccgcgcctaa agacaaaact
    31381 gtgaagaata acttcccaat aaaatcatag acatgtgcct gtccattaaa tgattcttga
    31441 agtaacggaa gactcaatcc gagataacgt tgtgtttgca aaatcaatgc tgctgccaca
    31501 actacagctc cacctatcgt atttcgccaa acaggatgag gaatccattg tgcatatcgc
    31561 gcttttaaaa agacgatcga acggctgaat acccaaccaa tcaaaccaaa agcaatccca
    31621 gcaacaaaaa ctttgaagaa cagcaacata ctccattcag gaatcacacc catttggtaa
    31681 tgtagatgag aaacaccata gctttctgtc atgaaatttg caaacagtgc agcaaagaag
    31741 ctagggaaga tcgcttctgt tcggactttc ccgattgcca acacttctaa tccaaataat
    31801 gttccagcta aaggtgtgcc aaatacagaa ctaaaacccg cactgattcc actgataatc
    31861 aagatttccc gttctgcttt atccaatcgg aacaaacgtg ctgtattatc tgctaacgat
    31921 ccccccattt ggactgctgt tccttcacgg ccaacagacc ccccaaataa atgggtagtt
    31981 atggttccaa acagtgtaag tgtaataagc cgcaacggga ttttttcttc tccaccattt
    32041 ccttgatcga tcactaagtt gtttccacga ctagaaagac cgccgtgata cgcatagaga
    32101 taagcaaaaa gcgcaccact aactggtagt agataaagga gccaaggatg tgatagtcgg
    32161 atatctgtca caactgtcag gctatttaag aaaaaagcag acaagctccc catgaatata
    32221 ccgagaatcg ctgtgatcac cagccacttc agcatgtaca ctaaaatgcg cattggactc
    32281 ggtaattgtt tttccataaa aaattcctcc tcaataaaaa aacgcctact aaagaaaggg
    32341 gctgtcccca gactttagta ggcgtcatta gctatctttc gttagcattt aaaggcgaac
    32401 accatcacct attcacttcc attaaaagat agcatatttt tctgtaaaaa acaataaaat
    32461 tatcgttctt tctttacacg ttcagcaaat tcatagaaag gagccaattt cgttaactgt
    32521 tgattacatt ggtcactatc atgacaaata taattcccct ttttcgtata cgtgccatca
    32581 gaacctgctt tcgttgtaga aagaaaaagc gacacattcg atgttttctg acagattgca
    32641 cagattccct tatttatcgt tggtgataaa gtaccgctaa tcccttttaa tttttcatct
    32701 tcgtaataga ccatgaattt tttctgcgta cctggatcat tccagccgat gtacgtatgt
    32761 tcttgcaagt cgatttcatt ccaagctggt tgtttgagtt tttttacttt tcggaataat
    32821 ttctcgattt gtggtttgct aggctgcata aacggctgaa cgatttgctt cagcttctcc
    32881 aatgctcttt ctgcttgcgc ttttgtcagt tttacgtcta aaactgtcgt taaaaatgta
    32941 tcgatttctg gcagttcgtg atttaatatg tctcgaatct tttcagctgc tagtgcctga
    33001 actgtttgta ccgtttttgg atcattgaca gatgagtacg catttaataa ctgttctact
    33061 tctcttttta taaaaaaata ttggtatggt tttattgttt gttccatgat tttaaagtcc
    33121 tttcttctaa ccaaagtgca tactttgtag aaaaaagtga tcttctgcaa agtatgtgtt
    33181 acctgaagat cccggactct tttaaataag atacgttttg catcttatca tcaccccctt
    33241 taatgcttat tcttctatgt acatgattca ttcgataaat aaaagtaaac cgtatcttga
    33301 acagatagtc aactgcaaac agaaaaaagg actgtgacaa aagtcgtgtc aaagtccctt
    33361 gtttcgaatg aatggtgttc aaaagcttgc atctgcggta ataagccgaa acttcacaaa
    33421 aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc acttctgtca
    33481 caacctcaat atggtctatt attggtttta cctgttaaat cgaggattct ttcccaaagt
    33541 aacgttgcca tctcttacgc atacttgcag acatgggctg agccgcttct ttgactgcgc
    33601 agtatttgtc cacgaatgtc acaatatatc cttctttata cttaggcggt gcgatcgttg
    33661 caccccacat gtgcttcaaa atgatatctc gctcaagatc cgacaaatcg gtgatttttt
    33721 ctgcattctt tacagcaatt cttggatgga tatacgcatg tgtcccctcg tcaaatttcg
    33781 tcgtacgcca atcatagtag aacagatcat gcaaaagtcc agcacgcgct gttgctttag
    33841 cattgccacc ccattttttt gcgagcagat aactatagta agaaacactg atggaatgct
    33901 ccaaacgagt ggaatgaaca tgctgtgtgt aattcgctaa ttttttcact gcttctgttt
    33961 ctaaaagatc ttctacataa gacatatatt cttcgtcttc acgccatttt tctgtcataa
    34021 ttatcgatct gccacctttt tgaatttcat tttcttctga aattgatcta agtataccac
    34081 cagtgttcgg attattctag tcgccttgga taaaatagcc aaagcttaat agttccttaa
    34141 tcataaagaa aaatgataaa gaacaatccc tgccgcaacc ccaacattca acgattccgc
    34201 atttcctttc atcgggatat atacgttctg atcagtcaaa gacaagattt cctgacttac
    34261 cccctgacct tcgttcccca tgattattgc gaagttatct gatttttcta ctttgtctaa
    34321 tgcgtgtgct tcttcattta attctgtgcc atacactgta aaccctttgt ctttcattaa
    34381 aggaatcact tctgtcaaat ctttttgcaa cacaggcaaa tgataattgc ttccctgcat
    34441 gctgcgcagc acctttgtac tataaatatc tgccgttcct ttgcctaata aaacaccagc
    34501 catatcaaat gcatctgccg tacggatcat cgtccctaca tttccaggat cttgtacatt
    34561 atctaacaac agccatcctc ctgaaaaatc tggcgtgcct tcagccgtta acgccatcac
    34621 tgctaagatt ccttgtggtg taggtagatc cgatacagca tccatgacct catccgaaac
    34681 tagaaaagta gaaatctctt tgtttgcggc cagccagtct ccccattctt tcatgcctcg
    34741 ctgtgtcaca aaaagttctt ttatttctgc atcagacgac gcagcttctt caatcagatg
    34801 gaacccttcc aaaagatatt cttccttttc ttcccgatat ttctttttat gtagtttttt
    34861 taattctttg attcttggat tttttgctga ttggatctct ttcacattcc tcacctcccc
    34921 atgattatat catggaatag gtgcttgagc ttgtttcatc ctaaaaaaat cggtatcatt
    34981 aatttataaa ataggaaaga aaggtgacga acatgcgcaa agttaaaatg aacgtccaag
    35041 gacgcgtcca aggcgtagga tttcgctaca tgaccaagat ggtcgctgat caactaggcg
    35101 tgactggaac ggtaaaaaac gaagatgacg gctcagtcac gatcgaagca atcggcaatg
    35161 acgacatcat ccagaaattt attgaagaag tcaagaaatc tccgagccca agtggacgag
    35221 tgcagtatgt ggatgtccaa gaagacccga tgattgagga acgaaagaaa tttgatgtgg
    35281 tgggataagg ttgtgaaaaa gctggtctgc atcaagcatt aaggacaggt tgtgaaaaat
    35341 agctctccat atttttcaac aggctggact taatgccgta gatgcaagct tttgaacacc
    35401 gtcgataagg ttattcttga ggcgctttgc ctcaaaaatt aaggacagca gtgaaaaagc
    35461 tgatctatca cttgcagtga gttcatttga ctccgctttt tcgggcagaa aaaaagtttt
    35521 tagtttatta aagtaagaaa gaaacacttg gaatcattct actttttcct agtatttctt
    35581 tcttgatttt ttgtcttttt ttcaaaattt ttcgctacgt aaaagaacga atatttatat
    35641 gaaatttcta tgaagttgat tgaattccat gcgctttttt agtatagtta agtttgtgta
    35701 aaaattatag aagaggaagt attctatgaa taaatggaaa aactggttat tgggatccgg
    35761 acttgtcagt atcctgctct ttttatctgg ctgtgtgcgg atggattcaa acggaaaccc
    35821 tgacacttcg ggaatcgttt atcgagtact cgtccatcct atgggccaag cgattacata
    35881 tctagttgaa aatttcaatt ggtcttatgg ctgggcagtt attgcaatga ctgtgatcgt
    35941 ccgtatcatc atcttgccac taggtattag tcaatcgaaa aaaacaatga tccaatctga
    36001 aaaaatgcaa gcgttaaaac cacaagttga agcagcccaa caaaaattga aagctgcaac
    36061 aacacgtgaa gaacaaatgg cagcacaagc agaaatgcag caagtatacc gcgaaaacgg
    36121 attgagtatg accggcggaa tcggctgtct gcctctattg atccaaatgc cgatcttctc
    36181 tgccttgtac tttacggcac gttatacaga aggtatccgg gaatcatctt tctacggcat
    36241 cgatttaggc tcaccaagta tggtacttgt tgccattgca ggggtcgctt atctgcttca
    36301 aggatacatc tcaacgatcg gtattcctga agaacagaaa aagacgatgc gtacgatgct
    36361 gatcgtctct cctgctatga tcgtcttcat gtcgatcagt gcgccagccg gtgttacact
    36421 ttactgggta gtcggtggtg tgttcagctg tctgcaaaca ttcatcacaa acgttatgat
    36481 gaaaccacgt ttgaaagcac aagtggcaga agagttgaaa caaaacccac caaaacaagt
    36541 cgtcactcca agaattgatg tgacagaatc agctaaacca gctgacagca aacaagcaac
    36601 caaacaaatc aagcaaccca aaaacagtac aaacaataaa ggccgcaacg caggaaagca
    36661 gcagaagcgg aagtagatag taaataagag tgtaataaaa attcattaac agttttagca
    36721 attaaaaata tcgaccaaga tttttgaaaa agaaatctgg tcgatatttt tatttaagtg
    36781 aatgtttaat ttgaatcatc gattgatcgc tatctaattg cagcagcatc tttttatgaa
    36841 gaaacaactt tttcttatct atcaacgtag acaggtgttg ctgtttcttc taatagtgta
    36901 cttaattcct taatattttg ttttccttct tctaacatcc aactactcgc tttttcttcg
    36961 ccttcggtaa caaaaggcgc cactcccttc gcccatgttg ctcgcccaca taagacacca
    37021 ttctccataa aggacaacgc ggagatacca cccatctaaa taacatccat aaacctttct
    37081 agtagaagaa tagtgatagt atccatccct ttttctactg ggaatacaat tacacgattc
    37141 aaagcttgat acacaacatt tcccttctta taaaccgcta ttgtaaccga attgttttga
    37201 atcacctttt tgggatagaa actggctcct ttccgcaact tcttaaatgt tggtttcttt
    37261 tatactttct ttgtagcaac tacaaatttc ttcaaatctc taaatgtttg cataacaacc
    37321 gcctttctga cttttactca ggatggtatt taaaaatcag caaaataagg cttctctcca
    37381 tcttttttat ttcctagctg ctttaaagac actcttgctg ttttttaatc tctatcaagg
    37441 gggctctgaa tctattttga tgaacttatc ttattaattt ctcaaatgag ctgtcagaca
    37501 caactgtatt ttttcaatct cataccggac aactatgcca gcttaaaaaa atttcctctg
    37561 gcaaaacttc tttattgaaa aatgacaatg aaaaaagacc attctttttg ttgaatgctc
    37621 attgtgtaaa catatttcct gcgactgctc ttcgaaccat tctcagtgca atccctaatt
    37681 tatctattaa atcttctttt agttcctcat ctttcatatt caaaatttcc tttgtatgtt
    37741 acacaagttt gttctctaaa ta
//
LOCUS       DASHRQ010000020        37284 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.020, whole genome shotgun sequence.
ACCESSION   DASHRQ010000020 DASHRQ010000000
VERSION     DASHRQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.020"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(180..2333)
                     /locus_tag="U2M88_001156"
     CDS             complement(180..2333)
                     /locus_tag="U2M88_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEM8373548.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2728..3576)
                     /locus_tag="U2M88_001157"
     CDS             complement(2728..3576)
                     /locus_tag="U2M88_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEM8373549.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3800..5740)
                     /gene="thrS"
                     /locus_tag="U2M88_001158"
     CDS             complement(3800..5740)
                     /gene="thrS"
                     /locus_tag="U2M88_001158"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEM8373550.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(6077..6256)
                     /locus_tag="U2M88_001159"
     CDS             complement(6077..6256)
                     /locus_tag="U2M88_001159"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEM8373551.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6303..8126)
                     /gene="cadA"
                     /locus_tag="U2M88_001160"
     CDS             complement(6303..8126)
                     /gene="cadA"
                     /locus_tag="U2M88_001160"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEM8373552.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8404..10683
                     /locus_tag="U2M88_001161"
     CDS             8404..10683
                     /locus_tag="U2M88_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM8373553.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10776..11423)
                     /locus_tag="U2M88_001162"
     CDS             complement(10776..11423)
                     /locus_tag="U2M88_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373554.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11602..12321)
                     /locus_tag="U2M88_001163"
     CDS             complement(11602..12321)
                     /locus_tag="U2M88_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8373555.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12452..13603)
                     /locus_tag="U2M88_001164"
     CDS             complement(12452..13603)
                     /locus_tag="U2M88_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEM8373556.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13820..14560)
                     /locus_tag="U2M88_001165"
     CDS             complement(13820..14560)
                     /locus_tag="U2M88_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM8373557.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14564..14887)
                     /gene="rsfS"
                     /locus_tag="U2M88_001166"
     CDS             complement(14564..14887)
                     /gene="rsfS"
                     /locus_tag="U2M88_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEM8373558.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14944..15537)
                     /gene="yqeK"
                     /locus_tag="U2M88_001167"
     CDS             complement(14944..15537)
                     /gene="yqeK"
                     /locus_tag="U2M88_001167"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEM8373559.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15521..16171)
                     /locus_tag="U2M88_001168"
     CDS             complement(15521..16171)
                     /locus_tag="U2M88_001168"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEM8373560.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16181..16492)
                     /gene="yhbY"
                     /locus_tag="U2M88_001169"
     CDS             complement(16181..16492)
                     /gene="yhbY"
                     /locus_tag="U2M88_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEM8373561.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16508..17617)
                     /gene="yqeH"
                     /locus_tag="U2M88_001170"
     CDS             complement(16508..17617)
                     /gene="yqeH"
                     /locus_tag="U2M88_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEM8373562.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17614..18141)
                     /locus_tag="U2M88_001171"
     CDS             complement(17614..18141)
                     /locus_tag="U2M88_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEM8373563.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18321..19106)
                     /locus_tag="U2M88_001172"
     CDS             complement(18321..19106)
                     /locus_tag="U2M88_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEM8373564.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(19099..19968)
                     /locus_tag="U2M88_001173"
     CDS             complement(19099..19968)
                     /locus_tag="U2M88_001173"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEM8373565.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19969..21348)
                     /locus_tag="U2M88_001174"
     CDS             complement(19969..21348)
                     /locus_tag="U2M88_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEM8373566.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21356..21775)
                     /gene="fabZ"
                     /locus_tag="U2M88_001175"
     CDS             complement(21356..21775)
                     /gene="fabZ"
                     /locus_tag="U2M88_001175"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEM8373567.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKTSAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21800..22276)
                     /gene="accB"
                     /locus_tag="U2M88_001176"
     CDS             complement(21800..22276)
                     /gene="accB"
                     /locus_tag="U2M88_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEM8373568.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22279..23514)
                     /gene="fabF"
                     /locus_tag="U2M88_001177"
     CDS             complement(22279..23514)
                     /gene="fabF"
                     /locus_tag="U2M88_001177"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEM8373569.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23533..24270)
                     /gene="fabG"
                     /locus_tag="U2M88_001178"
     CDS             complement(23533..24270)
                     /gene="fabG"
                     /locus_tag="U2M88_001178"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEM8373570.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24282..25199)
                     /gene="fabD"
                     /locus_tag="U2M88_001179"
     CDS             complement(24282..25199)
                     /gene="fabD"
                     /locus_tag="U2M88_001179"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEM8373571.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25306..25530)
                     /locus_tag="U2M88_001180"
     CDS             complement(25306..25530)
                     /locus_tag="U2M88_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEM8373572.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25583..26545)
                     /locus_tag="U2M88_001181"
     CDS             complement(25583..26545)
                     /locus_tag="U2M88_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEM8373573.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26555..26995)
                     /locus_tag="U2M88_001182"
     CDS             complement(26555..26995)
                     /locus_tag="U2M88_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM8373574.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27301..28266
                     /locus_tag="U2M88_001183"
     CDS             27301..28266
                     /locus_tag="U2M88_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEM8373575.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28323..28553)
                     /locus_tag="U2M88_001184"
     CDS             complement(28323..28553)
                     /locus_tag="U2M88_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEM8373576.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28639..29526)
                     /locus_tag="U2M88_001185"
     CDS             complement(28639..29526)
                     /locus_tag="U2M88_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEM8373577.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29615..30838)
                     /locus_tag="U2M88_001186"
     CDS             complement(29615..30838)
                     /locus_tag="U2M88_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEM8373578.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30855..31901)
                     /locus_tag="U2M88_001187"
     CDS             complement(30855..31901)
                     /locus_tag="U2M88_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEM8373579.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(32065..33792)
                     /gene="ptsP"
                     /locus_tag="U2M88_001188"
     CDS             complement(32065..33792)
                     /gene="ptsP"
                     /locus_tag="U2M88_001188"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEM8373580.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(33792..34058)
                     /locus_tag="U2M88_001189"
     CDS             complement(33792..34058)
                     /locus_tag="U2M88_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEM8373581.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34537..36771
                     /locus_tag="U2M88_001190"
     CDS             34537..36771
                     /locus_tag="U2M88_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM8373582.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36919..37239
                     /locus_tag="U2M88_001191"
     CDS             36919..37239
                     /locus_tag="U2M88_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEM8373583.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        10496 a         8062 c         6491 g        12235 t
ORIGIN      
        1 attagattcc acaaaaaaga ggttgtgaca atttttgtca caaccttttt tgccgagtaa
       61 acggtgttca aaagccagtt tctcggtatg aagtcaaatt taagcaaaaa tgtgagaagc
      121 tattttcaca aaatttttct tcatactcga aactaagcgg ctttttcacg acctcttttt
      181 tattttgcca tgaacatatc ataatacgcg tccattattt ctttagcaat cgtcaagttt
      241 atgtggtctt gttcgtctaa taagttcggc aagacaacac tgatggcaat ttccggattg
      301 tctgttggtc cgtatgccac gatattacta ttgactgtcg caatatctgg atgaccttcc
      361 gcaaccgttt ctgcagtccc tgtttttgcg gaaaggtcca ttttcgcact ggctaatggt
      421 ttggctgttg tgtaaggatc ggtaccatgg acaacttggt ggaatccttc acggaggatc
      481 gccatatttt ctgcagaaat attgaccgta ttttctactg ttgtgccaat ttctttcttc
      541 acttcaccca agtttccatt ttcgtcattg ccatagatcg ctttgactag gtgcggctgt
      601 actcgtttcc cgccattagc gactgttgaa gcatattgag ctaattggat tggtgtatat
      661 gtgtcaaact gtccaaatgc caaatcggta aagttacctg gagcatacca ttctttgcca
      721 ttatccgcat tgaacttctt catataatca acagaacgtg agatcccagg agattcattc
      781 ggcaaatcga tcccagtcgt tgtaccaaga ccaaactctt tgaaagagtc tctcaattgc
      841 tgataagctt tagcttcttc atctaaacta ggtagtccca ttccaggtgt atagtctagt
      901 cctaacatct ttaacgccac tttgatcata tacgtattag aagacaattc cgatgctttg
      961 actgcatcaa gagcaacttg tccgcttcga ttaaagacag agcttttttc tgaagcacct
     1021 tgcagacgaa tcggctcatc aatcaaaact tgatttccgc tgatcgcgtt acttgcccag
     1081 cctgctgtca aagtaccagc cttgactaca gaccctggat caaaggcgct agtaatcgtg
     1141 cctaaagcat tctcggtgat ttctttggaa ccttgttcat gactgaaacc agtcattgct
     1201 agcacttctc ctgtctgagg attcatagcc actgcataag caccaggcga atactgtgct
     1261 ttgccgttgt tgatcaatgt ttgataattc cttttcaaaa tttcttctac ttttgattgg
     1321 aattctgcat tcatagataa cattaaatta gaaccttttt ccccagagaa gatttctttt
     1381 tgattcgaaa catttccttc attatcaagg gaaacttcat attgggtttt ggttccttgc
     1441 aggacatctt catattgttt ttccaaataa ctttgtccta cacggtcatt ccgagaataa
     1501 ccttttgcta ggtatttctc tgcttcatct gctggaagtc cttgcttttc agtcgtcaca
     1561 ctgccaagaa tacttttcag tgaacttgct gcattatatt ctcgtgtcca gtctgtgcca
     1621 gtcgaaactc ctggaagttc agaagcgcgt tctgccacaa cagctaactc ttgatcagta
     1681 acaccagagt ttttgatata gacagtgttt aattcatatg cactattcat ccgtttaaag
     1741 atggtagcaa tttttaattg ctcctcgttt aaattaatct catcagctgt gactttattg
     1801 accatcgcac tatattgttc gctgttccct aattgctttt cttttgccga aagtcttttt
     1861 gtggcttctt tcaaatgttt gggatcagcc agccaaaaat cttttttatc tctatctgtc
     1921 agattttcat ctaccggaac atcgatcagc gagttcagct tcgttgcgat ttcaagcaaa
     1981 tctttagccg taacttgtgt tcctctcgta tatgtgatgg cctgattcgc tttattcgta
     2041 actaaagctt ttcctgtgga gtcgtagatc attccgcgag gagtactttc ttggaccgta
     2101 atcgtagaag tagacttcac tcgcgcctcc atctcttcgc cgttcacgat ctgcaaataa
     2161 cccaatctag caatcaacgc aacaaataac gtaaatataa tgaaaaaaag aaaatttaag
     2221 cgaaaaggaa catgggaccg gcgaaacgtt ttgttcttag gttcttgtgc accttttctt
     2281 attttttcta accaagaagt atttaatatt tttttcataa aatctttcat catttcctat
     2341 ccttccgcct atttacattc atgcttcatt gtacccaaaa acttcttaat aaacaagaag
     2401 tatttaatat ttttttcata aaatctttca tcatttccta tccttccgcc tatttacatt
     2461 catgcttcat tgtacccaaa aacttcttaa taaaaaagaa aaaatgggcg gagcaagaaa
     2521 ttctttacct aatttcaaga taaaatcaac tttttgtttc acagcaatca cttaaagtta
     2581 catatccaac tatttttttc attaaaaaat aaacttattt ttgaaaatta tgttttctta
     2641 agtcgtgaaa cactttgatg gatcatttat ttgtggaaaa ctacgttttg ttattctaaa
     2701 aaacttatat aaaaaatagt aaggttttta tttcgaatcc ataaaatatt cttggattgc
     2761 ttctcgtgtt tttttcactt tttcgctaag cttttcgttt ttagccatat agaaataaaa
     2821 aatgatatcg atcgtagcaa attgggccaa aagtgaatta gttgccgcac ttcgattcgt
     2881 gatttctttt ggtctagatg tttgtacgag tacatcggct atcttgctaa gcgggttatt
     2941 tccaaattgc gtcaacgcaa tgatttcgat attcatgctt ttcgccagct cggctaatgc
     3001 gactacatct gcacttctac ctgagtttga aacaagccaa agcactttct ttttctcatt
     3061 gctgacaagt tgcgggagca agacgtggat atctttttca aaaatgattg gcttaccgac
     3121 acgagaccat ttttgcagaa tatcttctac tactaaagaa gaggcgccaa ccccatataa
     3181 aaaaacagtc tctgccgatt ccaaacactc tacgactttt tggatcgttt cttcttctag
     3241 aatatctact gtttctttta tcgctacttg tgcattgtta ccaagtttgt ttttgataga
     3301 gtaaaaggac tcatttgctt ctatatcact aaaaccttca tcgattccag tctgtagttc
     3361 tgttgaaatc gtgattttga agtccgtcag actttgaaaa ccgatttttt tggaaaaacg
     3421 tacaactgtc ggcgcactca acccggaagc ttctgctaat tcattagctg tcattgtagg
     3481 gaccttctca atattaccga taatgtagtt ggccaacttt ttttctgctg aagaataatc
     3541 ttccatcata ctttcaatac gactaattac gctcataagt tcctccaccc ttttaatgat
     3601 agtctatgta agtataccat atcagaccct tattcacggt cgtatactgg ataaaaaaca
     3661 aaaaaacaat ccgcccaaca gaatatctta tcccatcaat cccacagatt ctattgagca
     3721 tctgtgtctt tgaggaatac ctgttatcta ttgttcggat tgctttctat ctactacttt
     3781 tttacaggaa atttacctat tagttttctc tgctgtaatt tttgacttct gcttccatag
     3841 catcaataaa gatattcaaa tcttctacag ctgtttcttt acttccataa cgacgtacgt
     3901 tcactgttgc atcttccaat tccttgtctc caacaactaa ttggtaaggg atcttttgtg
     3961 tttgagaggc acgtatctta tagcccattt tttcattacg atcatcaact tctacacgca
     4021 accccttcat ttgcaaacgt tctttgattt cataagcata atcgccatgt gcatcaacag
     4081 aaacagggat gattgttgct tgtataggtg ccaaccatgt tggaaaagcg cctttgtata
     4141 cttctgttaa ataagcgaca aaacgttcca ttgtagaaac aattccgcgg tggataacta
     4201 ctggacgatg cgtattttcc ccgtcttctc caacatatgt caaatcaaag cgctctggca
     4261 ataagaagtc taattgtata gttgataatg tttcttctgt accaagagct gttttgactt
     4321 gaacatctag ttttggtccg taaaaggctg cctcgccttc tgcttcaaag taatctagtt
     4381 ctaattcatc catagcggct ttcagcattg cttgggcttt ttcccacatt gcgtcatcat
     4441 caaaatattt atctgtattg tttggatcgc gatagctcaa gcggaaacga taatctgtaa
     4501 tattgaagtc agcatatact gccaccatca attcaagcgt acgtttaaat tcatctttga
     4561 tttgatcagg acgaacaaat gtatgaccat cattcaacgt catttctcgt acacgttgta
     4621 aacctgacaa agcacctgat ttttcatagc gatgcatcat tcctaattca gcgatacgaa
     4681 ttggtaattc acgataagaa tggatcgtgt ttttgtagac catcatgtga tgagggcagt
     4741 tcattgggcg aagaacaagc atttcgccat ctcccatatc cattggcggg aacatgtctt
     4801 cttgataatg atcccagtgt ccagatgttt tgtacaactc aacatctccc attattggtg
     4861 tatatacatg ttggtaacct agactaactt ctttgtctac gatataacgt tcgatcgtgc
     4921 gtcggattgt tgcccctttt ggaagccaga atggcaaacc agaacctact tctggggaaa
     4981 ccatgaacaa gtcaagttct tttcctagtt tacgatggtc tctttctttt gcttcttcac
     5041 ggagacggat atattctttc aaagctttct tgtcaaaaaa ggctgtcccg tatacacgtt
     5101 gcatcatttg attgtttgag tttccacgcc aataagcacc tgccactgat aataatttaa
     5161 atacttgtat acggccagtg gatggaacat gtggtccacg acacaaatcg atgaattcgc
     5221 cttgttgata agcagtaatc acttcttctt ctggcaattc actaatcagt tctactttgt
     5281 acggatcact tgcaaaaatt tctaatgctt cctgttttga aagaacacgg cgttcgatcg
     5341 gcagattttc tttgacgatt ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag
     5401 ttacttggtt ttgtccatta tctgtatcgt agtaaaaccc tgaatcgata gctggtccaa
     5461 cgccaaagtg gatatttgga tagaggcgac gcatggcttg ggccatcaaa tgagctgctg
     5521 aatgacggac taagccaagt gcatcttcat ggtctggtgt gatgatctca atacttgcat
     5581 cttcctcgat tggacgagtc aaatcaacga gttttccgtt cactttacca gctaaagctt
     5641 tttttgctaa gctttttgaa atgctttcag caattgccaa tgttgttgtt ccagactcaa
     5701 attctttgac tgcaccatct ggaaaagtaa tttttatcat aatagtttcc tccttgaaat
     5761 gttttgattg atcagatcat caagctgaaa aacaaaaaaa tcctagcatc tcttgaaaag
     5821 agatactagg acgaaaagtt cgtggttcca cctaatgttc aaacgcaaat gcgttcctca
     5881 agcgttgtaa cggtccgacc gcccttgctt caacaagggt ttcgaaagtg gtcatccttt
     5941 ggttttctcc tggaaaagtt tcagcctagc ttttcctctc ttatcgaaga ctctcaaatt
     6001 cagttggctt tctcatcaat caattatgtg tttatttaac cactaataaa tagaaaaatc
     6061 aagtgggaaa ctaaacttat tttttccatt ccccatttac accgtatgtt ccttttttca
     6121 tctcattgat gatgacatgg atgtgttctt ttggtgcctg cgtgttttta tgaacagctt
     6181 ccgtgatatc tttcatcata ttttctaatt gttccggact gcgaccttct actaattcga
     6241 catgtacgaa tggcataatg ctctctcctc tttctgatcg ttcttttttc aactcgattc
     6301 tatcatacta tgtttgtctt gggtactgaa tcaggaaaaa tggctggatc tttttttgct
     6361 ctcaaggcac tcaaaataga gaccgtatcc acgacttctt gcaaaacagc tccgaataat
     6421 gcggggatta gtcccgtact tgcaattaac atcaaaaaaa tacaaataaa gatgccaatc
     6481 agtacagact gtttcgctat tctcatcgta tctcttgaaa tcactactgc ttttgctaca
     6541 cgcaagagat catctttcaa gatgaccgca tctgcacttt cactagcagc tgtagaacca
     6601 tgagccccca ttgcaatacc gatatctgca gcagcaagtg ctggtgcgtc attcacgccg
     6661 tctcctacca tcgcaacagg tctttgatct ttagggatac tttctaaaat ggtcagttta
     6721 tcttgaggca agctacgggc atgtacagaa tctaattgga ctttttctgc aatcgtttga
     6781 gcaatggcag atccatcccc ggtgatcatc atcaaatttt tcacatcaag ttttcttagc
     6841 tccttgattg tctttgctgc ttctgggcga atctgatccg aaaataggat cgttcctgca
     6901 aaagatcgat caattgttac atagattgct gtattggacg tttctatttc ttcagctgaa
     6961 acaaaagagg cacgtccaat ctttacttca tgattattga ctactgcttg gattccttga
     7021 ccggtcactt ctttcagttg actaacagga tacagttggc tgtttttcac gttgttcact
     7081 aacgaacgag ctaagatatg gctagattcc tgttctgcac tagctgcata cagaagtagc
     7141 tcttcttttt ttattccatt gactggctga atcgcctcta cttccaatat ccctttggta
     7201 agcgttcctg ttttatcaaa agcaatcgtt tttgtacgtg cgagcttttc aatcgttgtt
     7261 ccagttttta caacgatccc gtttttactt gaacggctca ttcctgcaac taaagcaatg
     7321 ggcgcagcta gaatcagtgg acaaggtgat gccactacca gtacctcagc aaatcgtaaa
     7381 gggtcttttg agaaccacca agaaatacca ccgatcaaat aagcaatcaa cgtgaaaggg
     7441 actgcataac gatcagctaa acggacaaat cgggcaggct tttcttttga ttcttcgact
     7501 agtttaataa tcatttgata ctggctgtct gcggcacgtt tttcagcgat catttggatc
     7561 gatccgtctc catttaaaga ccctgacata atctgatctc caggtttctt ggtttccggg
     7621 cgggactcac ctgtcaaaga agattcatcg accgaagatt ctcctttggt cactcgacca
     7681 tctgcgggta caagttcgcc aggcttgact agtaactggt catcaatatt tatttcatcg
     7741 attactatat cctgcaattt tccttctttg aatcgatggg caattcttgg ggtgttatct
     7801 aacaaagact gcagttcttt acccgctcgt ttattcgcgt aatcctctag actatctccg
     7861 cccgtaagca tgatcaatat catcaaacta gcccagtatt ctttgacggc taatgttgca
     7921 aggattgcgg taatcgctaa tacatctacg ccatatcttc ctgaacgtaa cgtcttgatc
     7981 atttccaaaa acatcaagaa ggcgagaatc cctccagtaa atgcgacaat cgcaaatgca
     8041 agggccggct ggtgaaaaac aaattcactt aataaagcta tcccaccaat aagcaaagta
     8101 agtaaaaatt ttttgaaatg tgtcattgtt ttttctcctc ttttatgtga cttagagagg
     8161 ttagtaataa atttccagct gcctctttca tcttaagcat agtccttgac tagcactttt
     8221 taaaacaata ggccttaatt gaaaacaact atcactatca gcgtttccaa aattcatcag
     8281 ttctacaaac aaaaatattc agcaaggatt actttttcga ttcatcagtt aaaaagtacc
     8341 ttttttcgaa aaactatgct atacttttaa ttcgttattt acggcggaag gagcaaacat
     8401 gcgatgaatg agaaacaact agaacaaacg cacgtggatg aaacgatcga actaatccag
     8461 atcgaacaaa atatgctgga tacaaaacaa caagaattga ctcaggaaat gaatcattcg
     8521 acaaaagaca cagctaatca caagattcga gctggttcca atgaagcttt ttatgaatct
     8581 gctgttgaat atcgccaaca tgagcaggaa ttgcttttga aataccatac tcttgaagca
     8641 cagcaaaaac gcctgcaaac gttgtctgtc atgaaagata gtccttattt tgcccgtatc
     8701 gactttaaag aagaccgtga aacagaaaca ttatatctag gtatcgcttc tttaagagat
     8761 ctggcagaag aaacaatcgt tattgactgg cgcgcaccaa ttgccaatct ttattatgaa
     8821 ggtgagattg gtcccgcatt ttatgaaact gaaacagata gaatagatgt cgatctgcag
     8881 ttgaaacgac aatttaaaat ccaagaagga cgtattttat ctatggtaga tacgtcggaa
     8941 gtaatcaatg atgacttttt gctagagata ttggatgaat catcaagcaa ccatatgaaa
     9001 aacatcgtct ctacgataca aaaagcacaa aatgcaatca ttcgggacac aacaagtaaa
     9061 gtaatgctga ttgaaggaat cgccggaagt ggaaagacat ccgctttgct tcaacgtatt
     9121 gcttttctgt tgtatcataa tcgcaagtgg ctcgaggctg aaaacgttct tctattttca
     9181 cccaaccatt tgttttctga ttacatctcg actgtattgc cttcattagg tgaaagcggc
     9241 gtgcctacac agacattcaa aaattatttg gtgcagctgc tgccagagtt ttctttacta
     9301 gatgaacagc aacaagaaag tggttttttg ctaggggaaa aagatccgat acaagtgatg
     9361 aaaagcggat tgaccttagt cgatcagatt gatcgctata tccaaggcat cacaaactac
     9421 ggtcccttgt tccgtgatat gaaaataaat ggccgtacga tcctatcaaa ggaatcgatt
     9481 cgccaatggt ataaagaaac aaatgaacaa ttacctttac atcatagact ttctctatta
     9541 caaacgaagc tcctgaaaaa attaggcggt ctgcaaaaag acgaaacacg tcagcaatgg
     9601 gtcaaagatc tggcggaaga acagctccaa gaactatacg caacagatcc aaatcttgaa
     9661 tatacagaac aaaaggagcg ttcattacgt aaaaaactgg ccaaccaaat cgtacgtaaa
     9721 agattccgtc gaatccatcg agggatcatg aattatcaat ttgtcaatat aacgaaacaa
     9781 tatctccact ttctacaaac tgtgccactt tctcttctaa cacgacataa aatcagcgag
     9841 accaggtggc aggaacattt gacaactgtt cgtacacact tgcgtcaaag agaattgcgt
     9901 caagaggatg ctgtactatt ctttttactg atgcgccgaa catatccagt gacggttaca
     9961 cacaaagctc gctatatctt tattgatgaa atgcaggatt tcgcaccatc acaagtggca
    10021 ttgctgcaag ccttgttccc aaaagcaaat ttgactttct gtggagatct aaatcaaaat
    10081 gtctttggaa atgaaacgat cgttggatca ttggaagacc ttttcgtgga ccgtgaagtc
    10141 actcgtttcc aattaacgac aagctatcgc tccacaaaag aaatcactga ttttgctaat
    10201 cattttattg tgaaagaaca tcaagtagaa acaacagcaa gaaaaggaga ccttcctttg
    10261 ctcatccaag gaacaacaga tgatatcaaa ttgaattggt tagcagatac cattcgaacg
    10321 actcagggaa aagcccggta ttggcgtaca gcaatcatag ctaaaacaca agaagaatgc
    10381 gaagctcttt atgctcaact gccagacgag ttgaaacaac aagtccagct gattttaaat
    10441 gagagtgact ttatgaagcg ttccattatt ttacttccag cctttttagc aaaaggtctg
    10501 gagtttgacc gagttttctt gtgggatgta gatgcagaac atttttcttc tcagcaagac
    10561 aaattgatcc tttacaccat gtgtacacgt gcgatgcatg aattgaccct ttttacctcc
    10621 aataatgaaa gtcctttgtt aaacaatatg gatatttcat cttatgagaa aaaagtactg
    10681 taagtaaaaa gacaactgct atcattaatg ttagcagttg tctttttatt tacaataata
    10741 attgcagaaa aagtaattca atcatttaag gtcatttatt ctatatataa atccaaatct
    10801 gtttcaggat attttaattt gacaagcgaa ataatcattt tcagttcttc catgttttct
    10861 ggatattctg ggtcaaatac cttgctatga cggccaatcc cgccattgtc gatcaatcgg
    10921 taaattcgat tccattcaat gaattggtca attgattgga aattgctgat tggatagccg
    10981 ccacaatcca aaatctctga tacacgaaat gctaggtatg cactatcgat cactttattt
    11041 tttttcgtgt ctatcttctc atttctttcg ataagaattt tttcaggacg atgtttcaag
    11101 ctccactcta atagtagaaa gaaagcctcc atccgatact cttcttcaaa taaccgaaag
    11161 gctgaatcag aacgacgaaa aagcagcgga atcagataat tttgtctata aatcagcatt
    11221 ggtgcgcggt ccaccataca ctctaaataa cggttcagat accctgtacc gacatagttt
    11281 cctaatcgat agccttcaac gccgaatctt gcgtattttt gcgctaattt tgaacaatca
    11341 gttacgttca cagctaactc ttctactttg cggtaaccgc ttaagatacg ttcaaaaggt
    11401 tctaaagtga tttttgcttc catattcttc cccgtccatc ttccacatac ttcatatcat
    11461 ttcacatgat acttatgctc tcatcatacc acaggttgaa ttagaaaaga aaataaatca
    11521 cacaaaaaag caagacaatc tgttagtgag attgtcttgc ttttcgaaaa agactgaata
    11581 cttttcgtta aaactggttg cttagtcttc taatgccaca ttatggaaca catgctgtac
    11641 atcttctaga tcttccagtg catcaatcat tttttcaaat gtttcttgat cttctggaga
    11701 tagtgttacc tcgttttgag ggatcatttg gatctcagcg attgaaaaat cagtgattcc
    11761 tttttctttt aatgcttctt gtatactatg gaaatccgct acttcaccgt aaacgatgat
    11821 ttgtccttct tcttccatta catcgcgaac atcgatgtct ttttccatta ggtattcaag
    11881 gatctcgtca gcgtcttctc ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga
    11941 aaccgctccg gaaacaccca tgttcccgcc atttttccca aatgctgccc gtacgtctgc
    12001 tgctgttcga ttcacattgt tcgtcaatgt atcaacaatc accattgagc cgcttggtcc
    12061 aaatccttca tagcgcaatt cagagtatgt ttcatctcct gcaccttttg ctttttcgat
    12121 tgctcggtca ataatatgtt ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg
    12181 tagtttttgg tttgcatgcg gatcaggatc acctgatttt gctgccgcgt agatttcaat
    12241 cccaaatttt gcataaatcc ggctattatt agcatcttta gcagttttct ttgctacgat
    12301 atttgcccat ttacgtccca tattttcact tcgctttctg atttgatagt ttcataacgc
    12361 aaaaacgtcc tgttccatta tacttgtgaa tcacgattct gtgaagcttt ttttctttct
    12421 tccttcattc ttctcatttt tagtcaatta tctatactcg gattgggatt tgcccgaaat
    12481 tctgttccgg aatattgcct cccaattgat agatttgatc acttctaact aacagttccg
    12541 cttcttgtgc ctcttttttt ccgattcggg aataaataga aatctctttt ttttctttta
    12601 agaatcttct tccagcatcg gtaaatccaa gtagtcgaag ataattttta ttttgttgga
    12661 caggaatctc ttgctttttg atattcagca agacataaca agataatcgt tggatccgtg
    12721 tccatgtata tctttttgtt tttacctttg acacaaaatc ctcaaaacta tcagcagttg
    12781 cagctgctgt tttcatgcgc gattctattc cttcggtcat ctgatagata tctgtcagtt
    12841 ccagcaaagt agaagataac aaccgataac gcaaatacgg ccaataagat tcccaagtct
    12901 gcagcgaaca agttgaaaga tcttcttctg tctgaggcgg cacaagtcca gctattcttt
    12961 ttccttcttc taacgcatgg cgaatggctg ttgcactggc tatcctctga tctgtgatga
    13021 ttttttcatg atagcctgca cttttacgtc ggatcggatg taattccata gggtggacat
    13081 acttggcatt ttcctttgca taacttaatc ccaagatatg atttggcgaa gaaaaatcaa
    13141 gcgtaatatc gggatacaga ctgcgaaaaa cctcagtcat tttttgcgga taactatacg
    13201 tttgatcaga cagttcttga aatgcctctt ctatcctatc ttgattctct tggacaaagc
    13261 gtccgaaact gctgtaatca aacgaagtct cttcttctgt tccaaaacac aaactgtcac
    13321 agtccagtgt ttgcaagatt tttactgcac ctttagcaaa atagtcagct gactgaaccg
    13381 cccattcgac gggaagctcc actactaaat ccacaccatt ttgtaaagct tcattcgcgc
    13441 gatgccattt atcgatgata gccggttcgc ctctttgcaa aaaattcccg ctcattacag
    13501 caatgacgac ctctgctcct gttatttttc gtgccatttc cacatgatag cgatgtccat
    13561 tgtgaaaagg attgtactct acgataatcc cgcagctttt catgcgcatc ctccttcata
    13621 gttatcgata aaaaagaaat agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc
    13681 gaactatata aactattctc gcaaacacaa tacttctgcg acgaaaagct gcactgcaag
    13741 agcaatgcct atatcgttcg gatatataat ttatgttgaa gatttattct tttcttgtta
    13801 actcgagcag gcaaaaccgc tagtctttgt atgctacaaa gaaccagcgc tttgatttct
    13861 ctgatgctgc ttcgtctgta aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt
    13921 ccagcattct taaataactt tccaatggat aggtacgctc ttgatgtagt tcatcttcac
    13981 gaataaatcg accagaccct tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc
    14041 tgtgtttctc gcttcctgga taactatccc acaaaaaagc aaaattatcc ccttgataat
    14101 gatagctata ttctggaaaa acctcatcta cttgataggt ggagtgtacg tcaaataaaa
    14161 atcggccatt ttcttctaac gcttgataga cttcatcaaa cacttgctgt acctcttgtc
    14221 tatttttcat atagcacagc gaatcagaaa aacaggtaat tgcctgatat tgtccgattt
    14281 ctgataaatc gagcatattg ccttccacaa attggatcga tgcgtcttct tcatatgctc
    14341 gctggctggc aatcatcaac atctcttctg aaaggtctaa ccctgtcact ttatatccat
    14401 cttttgcaaa ggcaaccgct aatgctccag taccgcatgc cagttccaaa atattttttg
    14461 tgtcttcttg taaatgacgt ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca
    14521 tcacttcatc gtaaatgaaa gcaaatgttt cataagccat atgttaatcc aaccattcac
    14581 taagatcgac tagcggagca tctgaccata gtttttccag attatagaaa tttcgttctg
    14641 gcgcatggaa aacatgtacg atcacatctc caagatcgat caatacccat tttccgcctt
    14701 cttttccttc aatacgtttt acttcatatt tgttttcttc ttctttatca atgatttctt
    14761 ctgtaattgc gttgatttga cgttcgctat ttgctgaaca gatcatgaaa taatctgcca
    14821 acaaggaaac atttcgaacg tctaaagcga taatgtcttc tgcccgtttt gaatctgctg
    14881 cttcaatcgc aatttttaat tgttctttac tatctatgat gatttcctcc taatatgagc
    14941 agcttatttt tttgctaccc agtaattata tgtttcgatt gtttttggat aaatctgttg
    15001 ttcttgcgca agcaaatggg ccaatgtatg ttttgtttca aatgctacag cttcgtctag
    15061 atcagcccat gcaatggctc gtgcatcttg taccccagga aaatctcgac ctggttcaat
    15121 atagtctgct acataaatga ttttgtcaag gagactcatc ttagcagccc cagtcgtatg
    15181 cacccggatt gcatgaagaa tctctgcatc ggtgattcct aattcccgct ctacaaacga
    15241 agcgccaaca agaccatgcc agattgcatt attataattc aataactccg gatcaaaacc
    15301 gtctcttacg attaccattt tgaactcctc atctggtcgt tcttttgcat aatcatgtgt
    15361 caatgcagca atactagctt tttcaggaga agcaccgtat tttttggcaa gagccacggc
    15421 tgtttcttct actccaagca catgcttgaa gcgttgttcg ctcattctca tctgtatttt
    15481 ttgcatcagg ttttctctag aataagcagt atattcatta ctatagtcca tattcataca
    15541 acccttcatt ttgaatataa tcaattactt tatctggaac aaggtagcgg atagaacagc
    15601 cttctttgat cttttgacgt attttcgtgg aactgatgtc gatctcaggt acatctaccc
    15661 aaattacagg atatggagta tccgttgtgt atcctggtcg acgtataccc acaaagttga
    15721 ccatagaagt cagctcatcg attttatacc attttggcaa atattctacc atatccccac
    15781 cgataataaa gtaataatct gtatccggat tgttttgcgt caattccttc atagtatcat
    15841 aggtgtaact tttcccacca cgagctaatt caatcgtttc aatctgcaaa aaaggattgt
    15901 cttccaccgc taattccaac atgttgagtc gatgttttgc atcaatcgtc tgtttctcat
    15961 caacatgagg tggctgataa gaaggcatca aaaatacgcg gtcaagacct aaattccggc
    16021 cagcttgttc agccatcact aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga
    16081 tccctacttg ctttttcttc tctaagaatt gaggcatttc ttcaggaaat aattgtgaac
    16141 gaacaaatgt cttagcttgc gtattcatca tggtcactcc ttatattgct tttacttctt
    16201 ttgatagcct ttgatacttt tcttttgtgg aaggtttgta taatacaata acacgtccaa
    16261 tgatttggac gatttcgcaa tctatttctt tttctaatac ttgagctgct tcttctgcaa
    16321 tctcatctgt attttgaagt aacgatactt tgatcagttc tcttttttct aaagcttctt
    16381 caatttgaac gatcattgct gaattgatcc caccttttcc gatttgaaaa atcggctgaa
    16441 gatgatgcgc tttgctacgt aaaaaacgct tttgttttcc tcttaaactc ataattttcc
    16501 ttcttcctta aatcaatgct ttgcgtttca atacatctac accctttggc gcccagcccg
    16561 caattactac tggcgctgtt acagtgatcc agcctagacc ggcaaaaacg atgtctgttt
    16621 tttctttcac agaaaactca aaacgaacaa gctccggaaa tgcctcgact tcatcaggac
    16681 gaggaggctg taataatcca cctacatgtt tttgataaaa ttcgtctgct ttttccagtt
    16741 tcgttcgatg aatatttaca tcatttgaca cataagcaat aaatgaagca cgattgccgc
    16801 tgataaaatc aaatcttgca agtccgccca agaaaagtgt ttgcccttca ttcagctgat
    16861 aaactttagg tttgatttct tttaacggtg caatgatccg caaatctttt ttacctaagt
    16921 agtgcgccat ttgatggcga tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat
    16981 ctaaaggaat ctcgatttta tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat
    17041 cttgtactcc cgctgtattt ttgatgattt gattgataag cgtcgatttt ccgacatttg
    17101 ttactccgac gatatagaca tcccgtccat cgcggtattc ttcgattttt tctaatagtt
    17161 ccgtcatctc atgagatttc ttagcactag ttaataatac atctactgga cgaattcctg
    17221 cttcgtaagc acgttccttc atccattgtg ttaatttagt ttttttcaat gatttaggta
    17281 atatatccac tttgttccca actaaaagta ctggattgtc accgacaaaa cggtgtagtc
    17341 ctgggattag tgaaccattg aagtcaaaaa tatccaccac attgacgatc aatgcatctt
    17401 tttgccctaa ttcattcagt aagcgcaaaa agtcgtcatc cgtcaatgag acatcttggg
    17461 tctcattata atggcgtaat ctgaaacaac gctgacaata gacttccccg gaagctaaac
    17521 ctttttccaa agccgtttgc ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa
    17581 cggcaccaca gccgatacat tgtaattctt ctgtcattcg attccacctc gccagcccat
    17641 atcagggtgt tttgctaata gataggccat aattcttttt tcccaaaaac gattaaattg
    17701 tgtcttccaa ctatctgttg cgataatggg ttttacaagg atgctacgta tccctgcacg
    17761 attggctcca cgtatatcag tcataatctg atcgcctacc attactatct catcatctgc
    17821 taaatccaat tgtttctttg ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc
    17881 aacatacaaa agatcaaatt tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga
    17941 aacaacaaca acagggattc ctgcattttt catttctaaa atccaagtaa gcaactcttc
    18001 tgttccatct ggattattcc atgcaatcaa cgtattgtct aaatcggtca atactgcttt
    18061 gatgcctaga ttctttaatt gggcaggtgt aatctgatag atggcttcta ccatccatgt
    18121 tggtttgtaa tttgaaaaca tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa
    18181 gtactcctca accccgtatt atactgcata tcagcaaaaa aaactacctc tatactcgtt
    18241 cgtctttcag aaaaagcatt tctttacaaa atgaccaaaa ataaagattg atcacagaaa
    18301 aatttagaaa caaaaaacaa ctaatattgg cggaacctct tgtatcgttt ttccagtaat
    18361 ttatgctgat ctaatcgaac aagttccgtc aatctttcaa tcaacgcttt tttcataatg
    18421 tgtgcgactt tatatcgtgg caactgctcg ccgttgaacg tttcaggaat aactcgatca
    18481 acgattccta actcgcgtag ttctggcgca gtaattttca tcaactcagc tgcttctgcg
    18541 gcgcgactac cgtctttcca caaaatcgac gcaaatcctt ctggagaaag cacagcataa
    18601 atcgtttctt ccagcatcca gacttcatcc gctaaaccta gagcaagggc accgccactt
    18661 ccaccttcac caatgatgat ggcgatgatc ggtaccttta aatcagacat ttcgagcaga
    18721 tttctggcaa tagcctctcc ttcgccgcgc tcttctgctc ctacaccgca atatgcacct
    18781 gctgtattga tcaaggtaat caccggtcta ttgaactttt ccgcttgttt catcagtcgt
    18841 aaagcttttc gatagccttc aggatgggga gaaccaaaat ttctgcggat attttcctgc
    18901 agatcccgtc ctttttgaat accgatcact gttaccggtt tgccatctaa tcgagcgatt
    18961 ccaccaacaa tcgcttcgtc atctgcaaat aaacgatcac catggcattc ctcgaaagaa
    19021 tcgaagatct cttcaaaaaa atccaagctt gtccatcgtt ctttagctct tgctaactga
    19081 acgacatcat gtgctgtctt atccacgatc tatccatcct ttctgtgtat gcaattcaag
    19141 aagcgtatgg attttctgtt tcaattccat gcggggaaca atctgatcga caaaaccatg
    19201 agataataag aattcagcct tttggaaatc ttctggaagt tcctgcttga tcgtctgttc
    19261 gatgacgcgt cttccagcaa aaccgatcaa gctttggggt tctgctagaa taatatcgcc
    19321 ttccattgca aaactggcag ttactcctcc ggtcgtcggg tccgttaaaa cagttagata
    19381 aaaaagacct tcattgctat gccgtttgac agcagcagag atttttgcca tctgcattag
    19441 agaaaagatt ccttcttgca tccgtgcgcc acctgaagct gtgaacaaga cgactggaag
    19501 actttccttt gttgctcgtt cgaataatct ggtgatcttt tctccgacaa tcgttcccat
    19561 acttcccatg ataaaattag gatccatcac tccaataatg aagggaatcc cctgaatggt
    19621 tgctttacca gtgaggactg cttcatgaag tcctgtttta tcttgcattt ttttaatttt
    19681 ttccgtatat tcgggaaatg ctagcggatc tttggttaca agatccgtat cccattcttc
    19741 aaaacttttt tcatcaataa tcaaagctag acgctgccat gcaccgattc gaaaagcgta
    19801 tccgcaatga gggcatactt tttcttcacc gatgtctttc gtataaagga tatgtttgca
    19861 atttggacac tttgcccaca tgttatcagg aacagatggc gagttagcag gtttgctctc
    19921 tctattagga ttgatccgaa tatatttctt tttcttaaac agagccatct atgcgcctcc
    19981 tatctccgtt tctggtgtcc aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta
    20041 atcacctgct atgactgcag gatgactaat caaatccatc tgaaattctg cgttcgtgat
    20101 gattccttct gtgataaatt cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg
    20161 cgtatttcca tggacgatta tttttgcaat cattgaatca taatatggcg ggatcgtata
    20221 tcccgggtaa actgcactgt ctactcgcag tcccatccca cctgctggaa gcagtaagtt
    20281 ttggatcttc cctggagaag gagcaaaatg gaaagcagga ttttctgcat taatccggca
    20341 ttcgattgcg tgtccttgga atactacttc ttcttgacac acattcaaag gctcaccggc
    20401 tgctatctcc acctgttttt ttactaaatc gattcctgtg accatttctg ttactggatg
    20461 ttccacttgg attcgagtat tcatttccat gaaataaaaa tcgccttctt catccatcag
    20521 aaactcgatc gttccggcat tttcataatt gactgcttta gctgctcgaa cagcagcgtc
    20581 tcctagtgct tctctttttg tttgtgagat cactacagaa ggagattctt ccagtacttt
    20641 ttgattgttg cgttgtaaag aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg
    20701 atcacctaag atctgaactt ctatatggcg agcaggataa atgatttttt ccaaatacat
    20761 atcatcattt ccaaatgccg ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag
    20821 ctcttctttt gattgaactt tccgaatgcc tttcccgcca ccacctgcag ctgctttcag
    20881 catcactgga taaccaattc tgtctgctat ttccaatgct tcttctactg tagataatac
    20941 accagtactt cctggaatga ctgggacatt tgcttcttgc atcaattgac gcgcattgat
    21001 cttatttccc attgcatcga tcgttgcact tttaggacca ataaatgtga tattgcactc
    21061 ttcacacatg gaggcaaatc ggctattttc agataaaaaa ccaaatcctg gatgaattgc
    21121 ttcggctttt gtcacgattg cagcgctcag tacttcctga acattcagat atgaatcaac
    21181 ggcttttgcc gggccaatgc agattgcttc atctgctaac tgtgtatgaa gtgcttcggc
    21241 atctgcttct gaatatatcg ctacggtttg gatccctagt tcgcggcatg cacgaatgat
    21301 acgtacagcg atttcaccgc ggttagctat caatacttta gaaaacatat ttcacctatc
    21361 caatcataaa ggttaattcc gcttcggcta cttttttacc gtctacttta gcgattcctt
    21421 tgcctattcc ggctgaagtt ttcactttca atatctcaac ttcaaggtat aaggtatctc
    21481 ccggtgtaac tttcttacgg aattttgctt tgtccaatcc gccgaaataa gccgtctttc
    21541 ccttgaattg ttcaagagaa agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa
    21601 tcaatacccc tggcataaca ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga
    21661 tcgttacatt tttcttagca acgatccgtt ctccttcaac catttcttcc actcgatcaa
    21721 tcaaaagcat tggataacga tgtggaatga tttcttttat ttcttgtatg ttcattgttt
    21781 tccctctttc ctgctttgct tatttttgct gaatcctaaa gagtggctgt ccatattcga
    21841 caactgactc gttttctaca aggatttctg tgatcactcc gtcggcctca ctagttatct
    21901 cattcatcag tttcatcgct tcaataatgc agataacttc tcctttggca acttgatcac
    21961 ctactttttt gaaagctggt ttgtcgggcg ctggttgcag ataaacgatt cccacgattg
    22021 gcgaaacaac ctcgattcca tcaaatactg ccgattcttt ttctttcatt atttctggag
    22081 cagttgttgc ttgttcaggt atgacattga ctggctccga aactggcaac gtttcgtttg
    22141 tttgaaccat attcatgcct cgacttgttt tatttttatt catatacaat tcaaattgac
    22201 cttcacgcaa atcgaattcc gttagcgagg attgatcaaa ttgactgact aattctctga
    22261 tttcattgat atccaaaatt attcctccca gcgtttcatg cacagaaccg cattatgtcc
    22321 gccaaaccct aatgaattac tcattgcata agtaaaactt gcaggtttgc tttcgttttt
    22381 gacaactgtc aagtcaatct caggatcttg tttttctaca ttgatcgtag gtgggataaa
    22441 ttgatgttct aatgccagca aagttgccaa ggcttcgatc ccgccggctg ctcccaataa
    22501 atgtccagtc atcgatttcg tgctgctgac gtaagtatct ctgaaatttt cacctaatgc
    22561 gatttggatt gcttttgctt ctgcctggtc attggctggt gtacttgttc cgtgagcatt
    22621 gatatatccg acttgttccg gctgtacacc agcttcttcc atcgcaagct tcattgcttt
    22681 agatgcacca gaaccatctg gagtaggagc tgtcatatgg taagcgtcac agtttgcacc
    22741 gtatccaacg atttctccat agattttggc accgcgagct tgtgcatgtt ctaaagattc
    22801 aagaactaga acgcctgctc cttcacccat cacaaaacca ttccgatttt cgtcaaacgg
    22861 aatcgaacca cgattaggat cagttgattc tgagagtgct gttaaagctg caaatccagc
    22921 aatccccatc tcacaaatcg ttgcttctgt tcctccagca agaatgacat cagaatagcc
    22981 atgtttgatg ttacggaatg cttccccaat tgaattcgtt ccgctagcac aagctgttac
    23041 gatagctgta caaatacctt ttgcacctac acgcaaagca atatttccag ctgccatgtt
    23101 ggcaatcgcc attggtacga acattggaga aactcgtttt gcaccttttt catgtagacg
    23161 aataacttgg ttttcgattg ttggaagacc accgatccca gatccgatga tgacgccaaa
    23221 ccggtcttga ttcatttttt ctttatctaa accgctcatc tccattgctt ctaaagcagc
    23281 gtagattcca tagatcgaaa acatatccat tcgtttgcta tcttttttaa taaaatattt
    23341 atcgtaaggg aaatctttga cttcacccgc taaggtaatc cctgtttctg aagcatcaaa
    23401 tttagtgatc ggtgcgatgc cattcgtccc ctcttttaag ctattcaaaa agctgtctgc
    23461 gtcatttcca ataggtgaag tgactccata gccagtaatt accactcgat tcatttgttt
    23521 gctctccttt tcttatccgt gcatgaccaa tccgccatcg acattgatga cttgtccggt
    23581 gatatatgga ttttgtgcta agaaaactgc tgtttgtgca atatcttcta cttgtccgaa
    23641 acgctgcatc ggaatttgct tttccgcttg ttccttgact ttgtctgcta aaacttccgt
    23701 catatcagtt gtgatgaatc ctggcgcaat ggcattacat gtaatgcctc tagttgcagc
    23761 ttctcgtgcc acagattttg ttaatcctac tacgccagct ttactagctg cataatttgc
    23821 ttgaccaata tttccaatca acccagatac actagagaga ttaatgatgg ccccttctcg
    23881 ctgcttcatc atttttttca atacgtgctg cgtcatattg aaggttccaa tcaaattgat
    23941 atccagacat tttttgaaat cttctgcatc cattcgcatc acaagtttgt catttgtgat
    24001 tccggcattg tttactaaca catggatcga tccaagcttt tcttctgctt ctttgatcat
    24061 ttgtcccgct ttctcataat cagagatgtc accggaaact cctacgcatt ttacaccgaa
    24121 tgcttcgatt tcttctatct tttcttttgg aatctctccg cgaccattca aaatgatatt
    24181 tgctccagct tttgcaaaag ctaaggccat cgctttgccg attcctcttg tactacctgt
    24241 gataaataca ttttttcctt ttacatccat aacgttcctc cttatagtat tgctattgtt
    24301 tcatcaaatg tctgcttatt ctcaacacgc agaactggta ttgttttatc gatttttttc
    24361 ataaatccgc ttaatacttt tccggggccg acttcgatga cttgttcgat gccgattgtt
    24421 tttaacttat ggatactctc atagaaacga acaggttgca tgacttgctg ttcaagtagt
    24481 cccgcgattg tttctttctt cattatttcc gttgttgtgt tgcttatgat aggaaaagaa
    24541 ggttcagaga attgaatctg acttaggtcc ttcgccagtt tttttgctgc cggttctaaa
    24601 atagctgtat gaaaaggccc gctgacattt agagggatca ttcttttgaa ccctttttct
    24661 tttaatagta aaacagcttc gtctactgct ttttcttcgc caccaatcac gatctgctga
    24721 ggtgtattat aatttgcagg agatacgatg ccgtacttgc tagcttcatg acagctttcc
    24781 tcgatcgttt caatcggcgc attcatcact gcaaccattt tcccgcttcc agctggggca
    24841 gcttctgtca tataagcgcc tctttttgct actaacgcaa ctgcttcagt gaaggatagc
    24901 gcattactag ctactaatgc cgaatattct cctaaactca gtcctaaagc tgcatctggg
    24961 atgattccat gctccttcaa taagcgataa tacgcgatgc tgactgttag aatagctggc
    25021 tgtgtatatt gcgtctgatc caaacgttca ttttctgtaa agcaaagttc agccatatcg
    25081 tagccgagaa tctcacttgc ttcatcaaat gtctgcttta caacggcttc ttcatataaa
    25141 tcttttccca taccttgata ttgtgcacct tggccactaa ataagaatgc tgttttcatg
    25201 tttttctcca atatatccag aatatatgtt tgattcttcc tattcaaaaa ggccgagaca
    25261 ttcctatttc aggaaatgtc ccagccccca acctaatttt aatgattatg cagtttgttt
    25321 ttctacgtaa gtcactaaat cttgaaccgt tttgattccg tcttctgttt cgattttcac
    25381 gtcaaattca tcttcaattt cattgatgat ttggaataaa tccaagctgt cagcttctaa
    25441 gtcttcttgg atgtcagtag tcaatttgat ttcttcagtt tcttttccta attcttctgc
    25501 gatgatttct tgaatttttt cgaataccat aataatatat tcctccgata ttgtttgctt
    25561 tttattttta taatttaaca gtctacaact ccatgagaat acttccccat gttaaacctc
    25621 cgccatagcc tgtaaatatt actttctgct gttttcctaa ggtgatcttt ccactagaaa
    25681 ctgcttcatc caataagata gggatgcttg cagcagatgt attcccatat ttatccatat
    25741 tggtcaggaa cttttcttgc ggcatcttta cttttcgggc aattttctca ataattctga
    25801 ggtttgcctg atgcaaaagc agataatcaa ctggtttgtc tttcatgact tcttggatat
    25861 tgtttgcaac gtctcgtacg gcaaaatcaa aaatatctct gccagtcatt tgcaaataat
    25921 acgaaccttc tgaatcttct gaatagaacg gatttttatt ttgctggtag ccagcggtca
    25981 atgagctact tctggtaccg tcagctgcta atttttcttt cagcaatttt ttctgtggat
    26041 tcgcttctaa gagaatccca cctgctccat cgccaaacaa cacagcagtc gtacgatcag
    26101 accaatcgat tagcttagat agtgtttcac caccaatcac cagtccctta gttgagccgc
    26161 agcggatcat tttttcagcc atgcttaacg cataaacaaa tcctgagcac gcagcactga
    26221 tatcaaaagc gatagcatga gttgctccca atttcccttg tacttgtgct gctacagatg
    26281 gtgtcgtgta atcgggcgac attgttgcta ctaaaataaa atccaaatct ttagggttta
    26341 gatttttctg ttttatcaat ttttccgcca ctaaaatgca taaatcagaa gtctcctgat
    26401 ttgttacaca tcttcgttca cgaatacccg tccttgacga aatccattca tcagaggtat
    26461 ccatcatctt ggaaagatca ttattagtaa taatcttttc aggaaggacg ctggctgttg
    26521 cagtaatcat tccaaattgt tccattaaat atcctcactt atagtcttgc aagaagtcgt
    26581 gcaagttttt cagtgcatcc agcaaggcat gctgttcctc cgtagccatt ccgtctaaga
    26641 tgttcttgac catttcacga tgaaaatgct gatggacccg aaatagtaac tttccttttt
    26701 tcgttagtcc caactttaca acgcggcggt catcttcact gcggatacgt tcgacatagc
    26761 ctttttttac taacttgtta atagcagtgg tcaaggtccc aacagtaatt gatagttctt
    26821 tggctacttc tgaagttgtt tttttcttgt acatcccgat tgcctcgatc gtgtgcatct
    26881 ctgtgattga cagatctttg aattgagatt ttttcagctc agactcttcg attgtcaaaa
    26941 tatcattgaa gacactaaca agataatcat ttactgtttt taaatctggt tccatgttcc
    27001 accgtccact aattagtttg attatcaaat cttttgatgt tcaaactata tcttaacttt
    27061 tttcaaaatg caaccttaaa agatcatttt tttactatat gagctttttt taagatttta
    27121 ctcatttaaa acaactgata aatcagcatt tataatttct tttattagaa aaatcatcta
    27181 tctcacatat tttgattatc aaagtatttt ctgtgaacag aactatagaa ttccctaaaa
    27241 cttgtccatt atttcaaaat ttgttatagt ctttttattc aaagcgtaaa aggagatcct
    27301 atgttaaaag aaaaaatgaa aactctcccc atgctgccgg gtgtctatct tatgaaagac
    27361 cgagatggta aggtcatcta tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc
    27421 tattttcatc agaataaaca gcattcaaaa aaagtattac gtatgattca tcatataaca
    27481 gattttgatt tcgtcgtagt agacacggaa ttagatgctt tattgctcga gtgtcaattg
    27541 atccaacatt accgtccttt ttataaccga caaatgaatt atttcagtaa ttataattat
    27601 gtacatatta ccaataaggg atttgtatta acagatactc ctacggctcg aacatacggg
    27661 ccttttcgtc tctataaaaa aatgccgtct attttgcgta taatggaaga aacttatcaa
    27721 atgccttggt tgtctgaaat tagtttgtta gctttgcgag tccagcttcc tgacctacag
    27781 gaaatgactt ttgaacagaa aaaaaaggaa ctccaagggc tgtttcaagg ccgaaataaa
    27841 aagctactga cttacttgaa aaaaagacaa caacatttca tctatcagtt gaatttcgaa
    27901 aaagccggta tgcttcaaaa agatatcgaa ttagttactt attttattag acggatccag
    27961 gaacaaaagc agtttttaag aacaccatcg ctcaccttca gcatgccatt agcagcagat
    28021 gagagtcaga aaaagcacta cctcgtttgt tatggtcaac ttgcagaaac aatgattacc
    28081 tctggtgacg tttctcctga cttctattat gaaaagaaag aagctcattt atctttgaaa
    28141 cgtcaattgt caaaagaaga aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa
    28201 ttagaaaaag aacagtttga aattgaactg ttaaacaaaa aagaagctga aaagcaacta
    28261 aattagttgc ttctcggctt cttttaggca tcttcaactt caaatgtcaa ttatcctaaa
    28321 atttattgga ttaagtcata gatttccatc gctacgatat cgatattatc aaattcgtat
    28381 tgctgatgag tatctgtttc agttaattca aatgtatcgg tcgctttatc ataagttact
    28441 gtacacttct ctacgccttc tttttcaaaa cggcgaactt gcagttcatt atctgctgac
    28501 tcgaccatag cctccagtcg ttttatgatt gcgactaatt gtgaatgtgt catcggaggt
    28561 cctccttttc ttatttctgt tatattgtat caaaagcata gcctacttgc attgattttc
    28621 tctaaaaaaa ctcgtttttt atgatttttt ttctggtatt tttccttcag gccaccaaag
    28681 tttgatcaaa gcttgggttc cgtcattctc aaaaccttta tctgccaact cttcatatag
    28741 tttcaatgct tgggtagttg ctggcaatac aagatccatt ttttctgctt cttctaatgc
    28801 aatctttaaa tctttgataa aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa
    28861 aatacgagga gaataattgc ttaaagacca gttagctgca cttccacctg aaagcgtctc
    28921 aatcactttc tttaaatcaa gtccgttttt ttgtgcatag acaagcattt ccgtcattcc
    28981 tgtcatcgta cctgcaatca tgatctgatt agccattttt gtatgctgac ctttcccagc
    29041 agaaccatgc aatgtaaatg tcgtaccaaa ttctttgaat aaaggcagta cttgatcata
    29101 aacggcttct tctccaccga ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc
    29161 accggatacc ggtgcatcta acgcatctgc gcctttttct tttgctgttt ttgcaatttt
    29221 ttctgccagg cttggtgtac ttgtagtcaa atcaactaaa atatgtcctg agatatctgc
    29281 agaaaaaatc cccgactgtc catagtagat ttcttctaca tctttggggt aacctaccat
    29341 tgtaaagaga atattacttt gttctgctac cgcttttggt gtatctgccc agatagctcc
    29401 ttctgttact aaaggatcag cttttgcttt tgtccgatta tagacagcta cttcgtgtcc
    29461 tgcttctagt agatggcgtg cgacagcact tcccatgacg cctgttccga taaaaccaag
    29521 tttcattttt tcgacctcct aaaaatcaaa aatccttgct tctatgctag tatacgctag
    29581 aaacaaggat tttttcataa aaaatgtttg aattttatct tttaaatgaa tttaatttta
    29641 ttttgattcg ttcaatcgag tcttcttctt cttttcttcg catctcttct tcatgataaa
    29701 caaccatgtc ttgataaaat tcgatcatag cttcgccgaa atgtgtagat gaaatatcat
    29761 aaagtttagc ttctaaaact tttggatctt taggaacttc actggaaata taatcaataa
    29821 gtgctggtgc aaagtcttcg tcggtttcaa aagttttccc taatgaagga tgatcgaaca
    29881 atcggttcaa ataagcattt ccttccacga cacaaggtgt tccggctgcc atcgcttctg
    29941 tataagtaag cccttgtgtt tctgaagtag aagcactgac aaaataatct gccgctttat
    30001 aataaagtgc tacatcattg ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact
    30061 gcagttctga gatcaattcc tctaactctt ctacataagg tccttttcct acgataacta
    30121 atctggcgtt aggataagcg gagatgacct gaggaaaccc attaatcaag gcttggatat
    30181 ttttctcata agaaatcctg cttaaagaaa gaatcatcaa atggttttcc tgaagaccta
    30241 gtgactccct taatttgctg atatcctgct gagtgatatc agaacgtttg aatttttcaa
    30301 tgtcgatacc agtaggaatc acgcgaagcg gtgctattac accatagctg cgtaatgtat
    30361 caataacacg ttcacttgga caaactacac ctgtcgtatg atttgcaaat aatctagaca
    30421 agtattttac atgggattgc ctgatcactt ttcctttacc gatataatga aggtagtctt
    30481 catacatcgt atggtaagta tggatcagag gtattttcaa cttcttagct accatcttcc
    30541 caagtatccc tgctccgaat tctgtatggg tatgaataag atctagttct agttctttgg
    30601 caaccaaata agcataccac atgcctctaa caacaactct tcgatccttg aaagaaataa
    30661 atggtacact tggcattcga atcacatctt tttccaactt atccgcatta ggatcggtcg
    30721 ttgtaaaaat atagacttca tgcccatgtc tttctaattc ttgttttaat gttttgatcg
    30781 atgtagcaac tccgctgact tgcggaaagt aggtatcggt aaaaaagccg attttcatgt
    30841 ttttcgcctc caaactagtc cttgacctga tttacttttt ttaatttgct agagactttt
    30901 tcattcagta cactttcgta tacattttta agttcttcac cgattttatt gatacttcgc
    30961 tcttttgctg tttgataacc ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt
    31021 tggatcagtc ggataaattc ttcgtttgtc ttccccatat agcagttctt gctatcttct
    31081 aaccagccat tatacactgg aatatctcga acaagtacgt tttgtttgct tgctaaagct
    31141 tctaaaacaa cgatcccttc tgtctcttca taagaaggga aaaagaatag atctgcacct
    31201 gaatacgcac cttctatgat ctctccacga atgtaaccag gaaacaggac attttcagga
    31261 tgatcttctt ttacaatttt ccggatgttc cgaggaatcg agtacatggg aacatgacca
    31321 aaccaaataa atgtgtattc gggcatcttc ttagcaattt ctacgaaatc aatgattcct
    31381 ttacgttcga aaaacaaacc gacacaaatg attacttttt gatccggaga cagtttgaaa
    31441 tattctctga acttttgttc tttcattgga tcagggtgat atttctctaa gtcgatccca
    31501 ttggaaatag cttgaatcgg cacacggata ccatagcttt ccaacaactg ttttgaataa
    31561 ggtgttggcg tgatcagata gtccgctttt tggtacaatc ctaccagata tttttttacg
    31621 ataggagaaa gctggttcga tccgataaag gaatttcgga aatcttcctc tgtagaatgc
    31681 gcatgataga caactttttt tccttctctt ctggctttat tgaccatatt atgactattg
    31741 attccgtatg tattgatgtg caggatgtca taatcttctt tttcatctag tgtataagag
    31801 ataccgactt ccgtcagagc acgtttttga tgatcaagcg cacgcccgat tccagatttt
    31861 gcaagcatct tttcactttc aaaatataat aataccttca ccttacgtcc cccttcactt
    31921 atatagtata acatagccta caaactgtca ctcaaactta agccctattt ttgtctattc
    31981 ttactgacaa aaacgttttc ttaaaaaaga acaaaaaaac tggcgtagct cttttacaag
    32041 ccacaccagc ttttgatacg ttgtttattt tacatattca tgaacaagtt caacgacttc
    32101 ttccatcgta tcgcattctt tcaatgcacg atcagcaagt tcagccattt tcgctgtgtc
    32161 caagcgtttc attaaactac gcgttttaag aatagatgtc gcactcattg agaactcatc
    32221 tagacccata cctactagca atggaacagc tgtttgatct ccagccattt ctccacacat
    32281 acctgcccat tttccttcag cgtgtgctgc atcaattaca tttttgatca aacgtaaaat
    32341 tgatgggtta tatggttggt acaagtaaga aacgcgttcg ttcatacggt ctgctgccat
    32401 tgtgtattga atcaaatcgt tcgtacctac agagaagaaa tctacttctt ttgcaaactt
    32461 gtctgctaag actgctgctg ctggaatctc gatcatgatg ccgacttgga tcgtatcaga
    32521 tacttccttg ccttcactta tcaatttttg tttttcttct tcaaagatcg cttttgctgc
    32581 gcggaattct ttgagtgtag ctaccatagg gaacatgata cgtaaattac catgaacaga
    32641 tgcacgaagc aatgcacgca tttgtgtacg gaacatttcg tcgccttgtt cagataaact
    32701 gatacgcaat gcacgatatc ctaagaatgg attcatttca tgaggtaatt gcagatatgg
    32761 tagttcttta tctccaccga tgtccattgt acgaacaact acaggttttc cttccatacc
    32821 ttctaataca gctttgtaag cttcatattg gtcatcttct gttgggaagt ctggagaatc
    32881 catatacagg aattcagtac ggtacagacc aactgcttct ccaccattat tatggacacc
    32941 aactagatct tttggtgttc cgatgtttgc agctaattca aaatgcttgc cgtcagctgt
    33001 gacagtttct gcatttttta atttttccca ttctgctttt tgtgctgcgt aatctgcacc
    33061 tgctttttca aattcagctt tttgttcatc tgtaggatcg atgattacat cgccttcgat
    33121 cccgttgaca gccaaaatat cgccttcttt gactttagca gtaatttctt ttgtcccaac
    33181 gattgctgga atttctaaag aacgtgccat gatagcagaa tgtgaagtac gtccaccaat
    33241 gtctgttaca aacgctttta caaaattgcg atctaattgt gctgtatcac ttggagtcaa
    33301 gtcatgtgca acaacgacca cttcttcgtt gatcattgat ggatttggta aagtaacgcc
    33361 taataagtgc gctaaaatac gtttagcaac gtcacgaata tctgcagcac gctcttgcat
    33421 ataagcatta tcttccattg cttcgaacat accgatatac atatccgtta cttcttttag
    33481 cgctgattca gcatttacgc tgttgtcttt aatattttgc ttgatttgac ctaccatttc
    33541 tggatctgaa agaaccatca aatgggcatc aaatacttgc gcttctgctt cacctaagct
    33601 ttgcgctgct ttttcacgaa tttgctgcaa ttcttcagta gatttagcta atgcagcatc
    33661 caaacgagct tcttcagccg cagtatcttc tactgttgcc ttactgaatg ttaaatccgg
    33721 ttgaactagc aggtaagctt ttgcaacggc tacaccgtca ctagcggcaa tcccttttag
    33781 catttcaacc attattcaga taagccttct ttcttcattg tttcaacgat tgctgccatt
    33841 gcatcagctt cgtcagcacc ttctgcagta atagtaacat cagaaccttg acctacacct
    33901 aaagacataa cgcccatgat tgatttcaag tttactgatt tacctttata ctctaagttt
    33961 acatcagagt taaatttgct tgctgtttgt actaatagtg tagctggacg agcgtggatc
    34021 ccagtttctg ctactacgtg aaattctttc ttttccatat taatcggtct cctttaagtg
    34081 agtgttgttt ttatgtgagg gttttctttt ttagtaattc agagaatcct aacaaatgcc
    34141 accctttcat ctgtaaagat taacattttt tttcgttaga tacaacagtt ttcattcaat
    34201 ttcagataaa agtattcaaa ttattccgga ttctttttca tccttttctt cacattttat
    34261 aacaaacgtg tttatttcct ttttcttgtc gctttctctt ctcctgacgt ttatcattct
    34321 ttcctttgac cctctttgtt ttcatatgaa acgaattact tgcttccctt tttattttta
    34381 gtataataag agtcaaaggt caggaatggt caatagagat ttcacctttt ctacctcata
    34441 aatctcttaa aaaactaggg gttattcttt tacctcatga aaaacgagat gtaaaatatg
    34501 gatgtaccca tttttgtgaa aggacgtgaa tgattcatgc tttgtcaaaa ctgcggcaag
    34561 aatgaggcaa cgattcactt gtatgccact gtcaatggtc agcggacaca actcgattac
    34621 tgtcagagct gttatcaaaa aatcaaaaat caacaaaatg gaggtctgac taatatggcc
    34681 caaaatgatc catttggatt tggcagtttg gacgatcttt tccgttccat gtctcgccaa
    34741 atgcaacagc aaggaaatta tgagcaaaca ccaccaacac aatttggcgg aaacaacaac
    34801 ttcaatggtg gtcagccacc tcaaggcaat acagaaggtt tattgggaga atacggaatc
    34861 aatatcacag aacaagcacg acaaggtgat atcgatcctg tgatcggacg cgatgacgaa
    34921 atcaaacgag tcattgaaat tttaaatcgc cgaaccaaga acaatcctgt cttgattgga
    34981 gaaccaggtg ttggtaaaac agcagttgtt gaaggtttag cacaaaaaat cgtagatggt
    35041 gatgtaccgc aaaaattaat ggacaaagaa gtcattcgtt tagatgtcgt atctttagtc
    35101 caaggtactg gtatacgagg acaatttgaa gaacgtatgc aaaaattgat tgaagagatc
    35161 cgtcaagcag aaaatgtgat cctattcata gatgaagttc acgaaatagt cggtgctggt
    35221 tctgctggtg atggaaatat ggatgctggt aatattttaa aaccagcttt agctcgagga
    35281 gaattgcaaa tggttggtgc tactacctta aatgagtacc gcatcatcga aaaagacgct
    35341 gctttagaac gtcgtatgca acctgtacgg gtagatgaac caactgtcga agaaacgatc
    35401 agtattttga aaggtttgca aaaacgttat gaagactatc atcatgtaaa atatactgat
    35461 gaggccatcg aagcagccgc aactttatcc aatcgttacg tccaagatcg cttcttgccg
    35521 gataaagcca tcgatttgct agatgaaaca ggttctaaga aaaacttgac gatccaaatc
    35581 gttgatccta aaacaatcga gaaaaaactg caagaagcag aagaacaaaa agttttagct
    35641 tctcgagaag aagatttcga aaaagcagct tattatcgcg atcagatcaa taaacttcaa
    35701 aaaatgaaag aacgtcagct cactgaagag gaaacacctg tgattaccga aaaagatatg
    35761 gaaaaaatcg ttgaacaacg gacaggtatt ccagtgggtg aattaaaaga aaaagaacag
    35821 acacaactga aaaacttagc agatgacttg aaagctcatg tcatcggaca agataacgct
    35881 gtagatcgag tagcgaaagc gattcgccga aaccgtgttg gattaaacaa acagaatcgt
    35941 ccaatcggct ccttcttatt tgttggacca actggtgtag gtaaaacaga gcttgcgaaa
    36001 caacttgcct acgaactatt cggttcacaa gattcaatga ttcgtttcga tatgtctgaa
    36061 tatatggaaa aacatagtgt ctctaaattg attggttctc ctccaggtta tgtcggatac
    36121 gaagaagctg gtcaattaac agaaaaagta cggagaaatc cttacagtct tgtcttgtta
    36181 gatgaagtag aaaaagctca tccagatgtt ttgcatatgt tcttgcaaat cttagatgat
    36241 ggtcgattga cagatgcaca aggaagaacg gtcagcttta aagatacgat catcattatg
    36301 acaagtaatg ccggaactgg taaagtagaa gcaaacgttg gttttggtgc ggcacgcgaa
    36361 ggtgttactc gttctgtatt gaaccaatta aacaactact ttacaccaga atttctgaac
    36421 cgttttgacg ggatcatcga attcagtgca ttatcaaaag aaaacttgat gacaatcgtc
    36481 acactaatgc tagacgatgt caaccaaatg ctggcagcac agcaacttca catcgaagtt
    36541 cctacgaatg tcaaagaaaa attagttgac ttaggctatg atccatctat gggtgctcgt
    36601 cctcttcgac gcacgatcca agaacaaatc gaagatggta tcgctgaatt ttatcttgat
    36661 cacccttcta tccatgagtt gaaagcaaaa ctggataaag atggaaaaat catcgttact
    36721 tctaaaccag aacgtttggc taaagaatca gccgaagaaa cggctgaata aatccagttt
    36781 tatagcgtga gacggtattt ctttgagaaa tgccgtctca ttcttttttc agaaaagtca
    36841 aaatactgtt cattttataa aaaagaaatt ataataagag aaacaagcaa ttaaaaatac
    36901 ataaaagagg tgagcgccat gaaattggta aatgtaacaa acagccattc ccgtcttgta
    36961 ttaaaccagc ttgaaaacac tgatgcccat ctagtcaagg tctacacagc cggaaatacc
    37021 actattgtct acactgaagc ccctgaacat aatgaaattc tgctgatcaa tgacaaacgt
    37081 aaaatccagc cgaaagaaat cgaagatgct aaaaattatt tcttagataa aatcaaagac
    37141 gccgtttacc acgaggatga aatcaaagta atcgaattgg caggccttgt tgaaatctcg
    37201 attccaaaac atgaattagg cgctacatta gccgtgtaaa caaattttga acataataat
    37261 taaagagcgt gggacaaaag taaa
//
LOCUS       DASHRQ010000021        37177 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.021, whole genome shotgun sequence.
ACCESSION   DASHRQ010000021 DASHRQ010000000
VERSION     DASHRQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.021"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            476..1339
                     /locus_tag="U2M88_001192"
     CDS             476..1339
                     /locus_tag="U2M88_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEM8373584.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="U2M88_001193"
     CDS             complement(1468..3090)
                     /locus_tag="U2M88_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8373585.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="U2M88_001194"
     CDS             3339..3860
                     /locus_tag="U2M88_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEM8373586.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="U2M88_001195"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="U2M88_001195"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEM8373587.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="U2M88_001196"
     CDS             5941..6759
                     /locus_tag="U2M88_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEM8373588.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="U2M88_001197"
     CDS             complement(6862..7059)
                     /locus_tag="U2M88_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373589.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="U2M88_001198"
     CDS             7228..7806
                     /locus_tag="U2M88_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEM8373590.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="U2M88_001199"
     CDS             7901..8038
                     /locus_tag="U2M88_001199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373591.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="U2M88_001200"
     CDS             complement(8851..9354)
                     /locus_tag="U2M88_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEM8373592.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="U2M88_001201"
     CDS             9713..10738
                     /locus_tag="U2M88_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEM8373593.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11085..11963
                     /locus_tag="U2M88_001202"
     CDS             11085..11963
                     /locus_tag="U2M88_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HEM8373594.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12439..12555
                     /locus_tag="U2M88_001203"
     CDS             12439..12555
                     /locus_tag="U2M88_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEM8373595.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13483..13821
                     /locus_tag="U2M88_001204"
     CDS             13483..13821
                     /locus_tag="U2M88_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373596.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13915..14145
                     /locus_tag="U2M88_001205"
     CDS             13915..14145
                     /locus_tag="U2M88_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373597.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14545..14916
                     /locus_tag="U2M88_001206"
     CDS             14545..14916
                     /locus_tag="U2M88_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEM8373598.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15190..15882)
                     /locus_tag="U2M88_001207"
     CDS             complement(15190..15882)
                     /locus_tag="U2M88_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEM8373599.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15885..16670)
                     /locus_tag="U2M88_001208"
     CDS             complement(15885..16670)
                     /locus_tag="U2M88_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEM8373600.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16907..18307
                     /gene="lacG"
                     /locus_tag="U2M88_001209"
     CDS             16907..18307
                     /gene="lacG"
                     /locus_tag="U2M88_001209"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEM8373601.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18332..18649
                     /locus_tag="U2M88_001210"
     CDS             18332..18649
                     /locus_tag="U2M88_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEM8373602.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18662..20335
                     /locus_tag="U2M88_001211"
     CDS             18662..20335
                     /locus_tag="U2M88_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEM8373603.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20349..21980
                     /locus_tag="U2M88_001212"
     CDS             20349..21980
                     /locus_tag="U2M88_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEM8373604.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22852..24042
                     /locus_tag="U2M88_001213"
     CDS             22852..24042
                     /locus_tag="U2M88_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HEM8373605.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            24176..26365
                     /locus_tag="U2M88_001214"
     CDS             24176..26365
                     /locus_tag="U2M88_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM8373606.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            26362..28155
                     /locus_tag="U2M88_001215"
     CDS             26362..28155
                     /locus_tag="U2M88_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEM8373607.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            28355..28669
                     /locus_tag="U2M88_001216"
     CDS             28355..28669
                     /locus_tag="U2M88_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEM8373608.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            28690..29067
                     /locus_tag="U2M88_001217"
     CDS             28690..29067
                     /locus_tag="U2M88_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEM8373609.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            29077..29850
                     /locus_tag="U2M88_001218"
     CDS             29077..29850
                     /locus_tag="U2M88_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373610.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            29872..31275
                     /locus_tag="U2M88_001219"
     CDS             29872..31275
                     /locus_tag="U2M88_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEM8373611.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            31457..32641
                     /locus_tag="U2M88_001220"
     CDS             31457..32641
                     /locus_tag="U2M88_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEM8373612.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            32638..32928
                     /locus_tag="U2M88_001221"
     CDS             32638..32928
                     /locus_tag="U2M88_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373613.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            32925..33146
                     /locus_tag="U2M88_001222"
     CDS             32925..33146
                     /locus_tag="U2M88_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373614.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            33188..33967
                     /locus_tag="U2M88_001223"
     CDS             33188..33967
                     /locus_tag="U2M88_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HEM8373615.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            34034..34528
                     /locus_tag="U2M88_001224"
     CDS             34034..34528
                     /locus_tag="U2M88_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEM8373616.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            34584..35222
                     /locus_tag="U2M88_001225"
     CDS             34584..35222
                     /locus_tag="U2M88_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373617.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            35294..35689
                     /locus_tag="U2M88_001226"
     CDS             35294..35689
                     /locus_tag="U2M88_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM8373618.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            35673..>37177
                     /locus_tag="U2M88_001227"
     CDS             35673..>37177
                     /locus_tag="U2M88_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM8373619.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKA"
BASE COUNT        12163 a         6102 c         7302 g        11610 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt atatttattc tatcaaaaaa
    10921 ttttgtttat gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag
    10981 tcttacagat attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag
    11041 tatgtttata ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata
    11101 tgaaaagggg aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt
    11161 taactcttcc caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg
    11221 aggtgtttga gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa
    11281 agaaaataga caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg
    11341 cagcttctca aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt
    11401 tggcaacaat gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct
    11461 atcgctttga ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg
    11521 gtgaattgga gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag
    11581 gaaagcagtg cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa
    11641 gagatagtta cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc
    11701 aagtgatgta cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag
    11761 agtcaaaaat ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg
    11821 atagtggagt cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa
    11881 gtacgagttt acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg
    11941 gcttaggtat tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac
    12001 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc
    12061 aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc
    12121 ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac
    12181 acacagtacg ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag
    12241 agggcctttt ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc
    12301 aatcagctag tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca
    12361 tttaaaacac aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga
    12421 agaaagtggg tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa
    12481 agcccaaata tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat
    12541 agtgggaaaa ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg
    12601 gctattttca ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca
    12661 tttttaacat atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt
    12721 ccagttatat agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt
    12781 agttattttt caattccctt tcaacctctt attttgacca gtagtattga tcactttacc
    12841 ttttaacacc tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat
    12901 aatcaaaggt tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt
    12961 gcaaataacc attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca
    13021 gctcactcca tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga
    13081 aaaagttaac tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac
    13141 tattgctaga ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt
    13201 tgtgtattgg aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca
    13261 ctattacaag tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa
    13321 tgaatagttg tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc
    13381 aatttgtgat tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt
    13441 gtagtgttta ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca
    13501 agtgaggaac atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat
    13561 attgttccgg agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata
    13621 ggatggtata tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca
    13681 gtaggcttag agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact
    13741 gatgaaagtg acgagactga gatcatttta acggattact caaaaattgc tactgataaa
    13801 aagtatatat tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa
    13861 atttactaga tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg
    13921 gtagaaaagg gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa
    13981 aatgagtgga aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt
    14041 tacagagaag gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta
    14101 gctgaagtag aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat
    14161 gaaaaatttt gacacatatt atgttattgt tttttcgata tctttgctgt taatattact
    14221 aactttaatg cagtataaag agtatccaga gtattttatt ttaacttttg tatatttagt
    14281 aagtagctac acacttctat ataaattcag aagttcttct agagtttcta aagtttgttt
    14341 cttaattagc ttattgattc tataatattt ttgatttata taggaaatta aaaatattat
    14401 agaatcaaca ctcagagaac gggttggttt ttaatagatt taataagtac tgataatgaa
    14461 atttctcctg gttgggaaaa cgaagttggt taccctgtag aagaattttt tcaagattac
    14521 tttgcggagt atgccgatag gataatgaga gattacgtaa cagataaaac tcaagaagtc
    14581 atcaatcaat cccagtcatt aaatgaatta tctgataagg cgaaagaaga agcagttgcc
    14641 attatttctt cagggattag tgactatatt gatatgagtg gtgaaatcaa ttatggcgat
    14701 aaagacaaaa ttgtacgcat gataaaagct tttatcaaaa cttttgatgt gaaggcagat
    14761 cttaagttta atgatactta cctagtagga aaattaatta atatccttgg gacagagaaa
    14821 gatacggaaa cgtttatttc ctctttgtct atgttgttta cagataagca tttttcaact
    14881 cagcttggtg gagaggaact agtggaaatc ttttaaccat tacgcccagc attaataaaa
    14941 gtatatcaaa aaagtactcc tgatgatttt taagattggt ttgagaaata atgtgaaaac
    15001 actattttta atgttaatta tagatagaga tgaacgcgtc atatggttaa aagaatgaaa
    15061 aagtcaaaga tacactgcta tatttcacta aggatatagc agtgtatctt tcatgtcgtt
    15121 tcactggcag ttttttataa aaggaggtgt gcatttcagt tgcttttctt tggtagttag
    15181 ttgaatcgtt taagaattaa cggatgtggc agacaggtgc tctgtccaaa aaccagtaaa
    15241 atctttgatg gttccatctt tatttagctg tgcctgttca aatactgttt tgtttttacc
    15301 gaatggatca attgcaataa ttgttccgat ccccgcacgt ttggcagcaa gtaatcctga
    15361 gtaagcatct tcgataacaa gacaatcagc cggagctaag ctcagtttgt ctgccgcttt
    15421 aagaaaaata tccggttgcg gtttcccagg gaaagagccg tcgtcataga caatcttgtc
    15481 ccagtcaaac cagcgggcta aatcgaaaat atcaaaataa aaagcaacat tctctttgac
    15541 agtagccgtg gcaatcgtta atggaatctg ttggtcagtc agctggtcaa gagtcgcttc
    15601 aagccctttt gtcaactgaa gctcgttttc attttgcaag caaagcatac gataatagct
    15661 ttctttttct tctgacaata cggcgatctc agaggcggtc aaatgctctg aaatgaaatg
    15721 tttcaaaatc tccgtattcg tccgaccatg aatatttttt aaaatatcct cgtctgataa
    15781 accatctttt gtatgatcat gtatcatatg cagccaagca gcttcatgca gatgagagtc
    15841 caaaaacatt gttccgttga aatcaaatat aatccctttc atagttacgc tcctatagtg
    15901 gtattttcgg ttgaatcagc aatttgcaga tcgacatatt gtgcatagtg ctcttgaacc
    15961 tcccgcgaga tctggcgatc cgtaatgagc agatcgaaat catgcaaatt ataaaacaca
    16021 taaaaatcat ctcgattgaa ttttttatta tcaacgagta gatatttctt acgggaatta
    16081 tttagagcga tctgctgtgc ttctccttct tcggtactgt aagtagagat cgcttcgttg
    16141 tgaacgccat ttgaactgat aaatgctttt gtaaacttta gtcttcttaa attgtcgttg
    16201 gtgatcggtc cgacaaatgc gccggtattt ttccggaaat caccgccaat cagtagaatt
    16261 tctggtccat ctgcttgagc aagaatatca aaaactggat aattatttgt aatgatccga
    16321 attttcttgt tcagtaaatg cgtcgccaat agttcgattg ttgtacctgg tcctaagaaa
    16381 atcgtttctc cctcgtgaat caaggaggct gctgtggcag caatttcttt tttttcttga
    16441 atctgcagag tggctttttc agtgtgagaa agttcttgat ctaaagtgta ggaaagactt
    16501 tgagcccctc catggacacg cagcagcttg cccgattttt ctaattcatc taaatctctg
    16561 cgaacggtca tatcagaaac atctaattga ttgatgattt cattaacggt aaggattcct
    16621 tttttattga ccatttctac gatagtaagc aatctttctt ttttcaacat tgtcttcacc
    16681 tctaatttaa ggataatcta tcatataaac ggacaacagt caatctaatt cgaacaaaag
    16741 taaacattta gggtggtttt tatgtttgtt acaatagatt ttattgctgt tttctggttt
    16801 ttatgattat ttgtgtttgt ttttgttgac aaatgttttg gatgcgttta caatgcaatc
    16861 agttagaaag agcgaaaact gattatagat ttgaggagag atgtacatgc ttaaattacc
    16921 agaagatttt atttttggcg gagccacagc agcctatcag gttgaaggag ccactaagga
    16981 aggtggcaaa ggcgctgttg cttgggatga ctttttagag gaacaagggc gattcagtcc
    17041 cgatccggca agtgattttt atcatcaata tgcaaaggac attgaattgt gtgaacgttt
    17101 tggtgtaaat ggtctaagat tatccattgc ttggagccgc atttttcctg atggagctgg
    17161 gaaaccaaat ccagaaggaa tcgcttttta tcatcgtgtg ttcgaagaat gtaagaaaag
    17221 aaacgtaacg ccgtttgtga cgttgcatca ttttgatacc cctaaacgtt tgtttgacca
    17281 tggagatttt ttgaatcgag aaacaataga agcatttgtt tcctatgcca ttttttgttt
    17341 tcatgaattc aaagaagtca aggtgtggag tacctttaat gaaatctatc cagttgcaac
    17401 caatcaatat ttgttaggtg ttttcccacc aggaatcaaa tatgatttta caaagatcgt
    17461 cgcctgtctt cataatatga tggttgctca tgcccgtgta gtgaactatt tcaaagaaaa
    17521 cgaacttccg ggtgaaattg gtgtagttca ctcattagag acgaaatatg cggcaacaga
    17581 cgcgccggaa gataagcatg ctgccttttt agacgatgca ttgtcgatcc gtttcttatt
    17641 agatgctacc tatttaggct attattcaac tgaaaccttg actgctcttg atgagatatg
    17701 tgaagcaaat caggccagct atcattttcc tgaagaagat ttcgttgaat tgaaaaaagc
    17761 atccacgcgt aatgactact tagggatcaa tcactaccaa tgccattttg tcaaagctta
    17821 cgatggagaa aatgcaatcc atcataacgg tacgggtgaa aaagggacct ctgtttataa
    17881 agtgaaggga atcggagaac gtatttataa agaaggaatt cctcgaactg attgggactg
    17941 gctgatttat ccagaaggtc tatatgactt gttgctgcga attaaaagcg attacccaca
    18001 ttacaacaaa atctatatca ctgagaacgg gatggggtat aaagatcagt ttgaagatgg
    18061 cattattatg gatcagccga gaatcgacta tttgagagtc tatttggaat ccttaagcaa
    18121 ggcgataaca gccggggtaa atgtgaaggg gtattttttg tggtcgctta tggatctttt
    18181 ctcatggaca aatgggtata acaaaaggta tggcttgttt tatgttgatt ttgaaaccca
    18241 gaagcgttac ccaaaagaat cagcttactg gtacaagttg gtcagtgaaa ccaaaacgat
    18301 tatttaataa gtgataatgg aggaagaaaa gatgaataaa gaggaattac aaatgcttgg
    18361 ttttgagatc gttgcatatt caggcgacgc tcgcagtaca ttattgaaat tattgaaaga
    18421 agtaagacaa ggaaactttg aacatgttga ttcggcgttg aaagaagccg acgaaaattt
    18481 aacccttgca cataattcgc aaacaaaaat tttagcggaa gaagcatctg ggaaagagat
    18541 ggagatggga ttcattttta tccatgggca ggatcattta atgaccactt tattgctgag
    18601 agatctgatt caagacttta ttgtactgta cagacagaat caaggatagg agaagaacgc
    18661 tatggatgga atcattaaac aaatcgaaaa agggaaacca ttttttgaaa aaatctcacg
    18721 taatagttat ttaggagccg ttcgtgacgg atttttaaca gcgatgccag ctattttatt
    18781 ttcaagcatt ttcattttga ttgctgcggt tcctgagatt tttggttacc aatggcctga
    18841 atctgtctcc acatggctat ggaaagtcta taattattcg atgggtgttg tagggttact
    18901 cgtttcagca accactgctc gctgcttggc tgattcgatg aatcgtcgct taccgaaaaa
    18961 caaagtaatc aacaccacct ctgtgatgct ggcctctgtg gtaagtttcc tgttgttaag
    19021 cgtctctccg attgaaggtg gattttcaac ggaatatatg gggacaaagg gactactggc
    19081 ttcctttgtc gctgcatttt taacagttaa tttatataag ttttgtgtgc tgaaagatat
    19141 taccattaaa atgcctaaag aagttcctgg aacgatttcg caaaccttcc gagatatttt
    19201 tcctttctct ttttcagtat ttgcggccgt tttaattgat ttaggcattc gacaaatctt
    19261 ccaaatctca tttgcagaag caatcattac ggtgctgcag ccgctattta cagcagctga
    19321 cgggtactta ggaatcgcta ttatctgggg agcgatggcg ctgttttggt ttgtcggtgt
    19381 tcatggaccg tcgatcgtgg agccggcaat tgcggcaatt atctatgcca atgtggaaac
    19441 caatctgcaa ttgttcaatt ccggtgaaca agcttctaaa gtactgacgg tgggtttagg
    19501 gaattttgta ggaactatgg gaggaactgg ggcaacattg gttgttcctt ttatcttcct
    19561 actctttgct aagtctaagc agttgaaggc agttgggaaa gcttccttta ttccagtaag
    19621 tttcgctgtc aacgaaccat tgctatttgc ggcacccatg attttgaacc cttatttttt
    19681 tattccattt ttgttaacac ctgttgtaaa cgtttgcctg ttcaagtttt ttgtcgatgt
    19741 attgggcatg aacagcttta tgtacgtttt gccttgggca actccagcac cgatcggttt
    19801 ggttctagga acgggcatgg gtatcttagc ttttgttcta gcagcggttc taatcgtcgt
    19861 tgacttcctg atctatctac cattttgcaa agcatacgat gctgtcttag tagagaaaga
    19921 agcacaaaaa gcagaggtga tcgcaaccga ggaaggtgct tctgccgatg tacgtgagga
    19981 agtacctgtg gcagcgagtc aagttatcag cgatgcaaat aaagaaatca atgtgttggt
    20041 actttgtgct ggcgcaggga caagtgcgat gttagctaat gctttaaaaa aaggtgcatc
    20101 tgaatcgaaa gcaccgatca gtgcaatggc gggtgcttat ggttctcatt acgaaatgct
    20161 gcaggatttt gatatggtgg tcttagcacc gcaagtcaat tcttactatg atgatatcaa
    20221 agaagacacc gatcgcttag gaatcaagct ggttgcgaca aaaggagcag aatatatcgg
    20281 attaacgaga gatcctgaaa aagctgttca gtttattctg aaccaatttc aataaaggag
    20341 gagccataat gaaaatggga atttggttgc gtaaagctgt tttatttgca gctgtgttag
    20401 gggctttggg ggctgtttca aaaactgttg aagcatctgc cagcgaattt attcgcggag
    20461 cagatatttc gatcctgtca gatatggaaa aatctggtgc aaaatattat gaagacgata
    20521 tttcaaaaga tgccttagag attttaaaaa ataatggtgt caactatgtt cgactgcgtt
    20581 tgtggcagga tccttataat agtaatggag aaagttatgg agcagggaca aatgatctgg
    20641 aaacgacgat tgaccttgca aaacgagcaa aaaatctggg acttaaggtg ctgctggatt
    20701 ttcactacag tgatttttgg gttgatcctg ggaaacagaa tcttcctaaa gcatggcaag
    20761 gtctgacctt tgaagaaatg aatacggcat tgtacgacta tacaaagaac gttctgtcgg
    20821 aaatgaagca gctggacgtt tatcctgata tggttcagat cggcaatgaa ctgaatagcg
    20881 gaatgctctg gccttatggc aagtcttggg gagaaggcgg gggagaattt gatcgattgg
    20941 ctgctttttt gaagtctggt atccaagctg ttcgagacac tcaacctaaa acaactccag
    21001 taatgctcca cttagctgat ggtggcgata caggagcatt tacttggtgg tttgatgaaa
    21061 tcacgagtcg aggtgtgtca tttgatttaa ttggagtgtc gtattatccg tattggcacg
    21121 gttcaatgaa tgaacttcaa accaatatga atgcgatcag ccaacggtat ggtaaagaag
    21181 tgatcgtagt tgaaacagct tatgggcata caacagcgaa tgccgatact atgccaaatg
    21241 cttttggtga agccgaagca gctgcggggg gatatgaacc aacaccgaca ggacaagcag
    21301 agtatttgct ggatcttgcc gatcggattc aggcagtacc gaataatcgc ggggcgggct
    21361 tcttctattg ggaacctctt tggtacaacg gaaatgtgag ttgggcaaca caagcaggta
    21421 tgaattatct cagtgttcaa tccactatgg gaaacgaatg ggacaaccaa gcgatgtttg
    21481 attttgctgg aaacgccttg ccaagcttgc gtgcgtttaa acaagcaggg gcgcaaataa
    21541 atctagttaa aaatgcaagt ttcgaaagcg atggaccaac gaccagtcct tccaattggc
    21601 aaacctggtt tcaacaagga accgctaagg gtacgatcaa aacggaagcg ggcacttttt
    21661 ttggggacta caaactaact ttttggcagg atcaagcata cgaggcatca gcttatcaaa
    21721 atgttagtgg cataagcaaa ggaacttaca cattgagtgc ctatgtaatg tctgggggag
    21781 agtttgatac taatcagtta tatatcaaaa attatggtgg agcggaacta aatactgcgg
    21841 ttgttaaaac aagtgaatgg acaaaaatta caatcaaaga cattccaatt acaacaggtg
    21901 tttgtgaaat tggggtttat agcaaggctt caggaaatgc ttggacgaac gttgattttg
    21961 tttcattggt gaaacaatag actgcggatc gtttcgattc aagatcggag gttcccttca
    22021 gccacatcgt tagcgtagaa ggcaggaatc gaacctgcac ctcgcctttg ccaaaaataa
    22081 ccagaaacga tttccagtca tttttaccaa aacgagtaaa gcaccagact tcatctacac
    22141 tttgtattat gctaaagaga gttcaacgat ttcgtaagaa gcaatgaaaa aattgcaaaa
    22201 agttgtgcag ataacataaa gtttgtttaa gaaagggtaa ccttagaatt ccgtcgtgaa
    22261 tatgatttga gcacttagat ggatagaaaa aagttctcgg aaaacagttc gtttttcgag
    22321 aacttctttt tatgataaga atgaaacaaa ttaacttata ttatagtctc ttttggtagg
    22381 gaaccatctt atttttaatg aaaatcaagc attttagcta ttgaagaatg ttgatttata
    22441 gggattcccc ctccatatta tgttggctct ttattcccac tctgttttca acgtaaagtg
    22501 tataaaacag aatgcgatac tatcaaggga gttaaaacaa tatacagaac agtaaaacac
    22561 tataaataaa aataaaagtg caataaaagt gcaatttttg cacctaatca tactctgttt
    22621 atatatgaaa actagattct gaatccaata tttggttaac gagaggcgtt cgaatttgaa
    22681 cgtcttttat atattacttt caaataaata tgaggtagaa tactatctat tttcttttta
    22741 acccaattgt tataatggag agtgtaaaat attttgtgta aatagaaaaa aggaagtccc
    22801 ttctgtagaa tagagttacc acaacacatt cacagaagag aggacttccc tatgaacgat
    22861 tttactacag aaattctaaa gactctagcg aacaaaggcg atttgaatga attattccgt
    22921 gtccatttgg agaaagcagt caatacgctt ctcaaaacgg agttaacggc tttcctagat
    22981 tacgaaaagt atgatcgcat tggttttaac acgggtaatt ctcgtaacgg ctcctatgac
    23041 cgtacggtca agaccgagta tggggaactt catctccaga ttccgcgcga ccgcaatggt
    23101 gagttcaagc aacagactgt tcctgcttat agacggacga atgacacgtt agaggagacc
    23161 gtcattcacc tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa
    23221 atgtatgggc attactacac gccccaaacc atgtccaata taacaaaatc atttacagaa
    23281 gaggtaacgg cgtttaatgg gcgggagctt catgaccgtt atgctgctat ttatatggac
    23341 gcaacgtata ttccgttaaa gcggaaaacc gtcgccaagg aagctattca tatcgcagtt
    23401 ggcattcgcc cggacggatc aaaggaagta ttgagctatg cgattgcacc gactgaatcc
    23461 atcacgattt gggaggaaat tttattggac cttcaagagc gcggtttgaa aaatgtcctc
    23521 ctgttcatca cggatggctt aaaggggatg gtaggagcga ttagtcggtt ctatcccaaa
    23581 gctcgttttc aacattgttg tgtacacgtt tcccgtaata tcagtcataa agtgcgtgtc
    23641 gatgatcgta aggaagtctg tgatgatttt aaaatggtgt atcaagcctc atctaaagag
    23701 gtggcgttgg aagcacgtgg tacttttgcg gaaaaatgga aaaccagcta tccaaaagtg
    23761 gttgaatcga ttctttcgaa cgatcacttg ctcactttct atgatttccc cttggccata
    23821 cgcaagagta tttattctac gaacttgatt gaatccttta ataaacaaat caagaaatac
    23881 agccaccgca aggaacagtt ccaaaatgaa gagtcgatgg aacgtttctt agtctcgtct
    23941 tttgatactt acaaccaaaa attcctaggt cgcagtcata aaggtttcca acaggccgaa
    24001 ggcgagctcg aacaaatgct aagccaactg attgagaatt agaaacaatc tgcagtaggg
    24061 aagaatcatt tacacaaaat tattgacgct cccttataat gagaactttt actaacataa
    24121 tttatgataa acttactata taatcatatt aattaaatag ttaggagatc tgcttatgtt
    24181 tatatctact cttcaaattc gaaattttag aaattatgaa tccgaaatct ttcgattcga
    24241 taatgagaca aatactatta taggtgagaa tgattcaggg aaaaccaatg ctcttactgc
    24301 attgagaatc ttattagatg atagttatta ctatagtagt aaaacactaa aagaatcaga
    24361 tttttttcat ggaattcaaa atgggtggca agggcattgg attattatct ctgcgacatt
    24421 tgaaggaatc agcgaagaag aatttgacaa tgagatttgt gcttccattt ctttaaattc
    24481 tgaaagtcaa acaatcttag aagaattgat ttcaaatgca gataaaggtg taggtagtat
    24541 ttctctattt attagaccta acaaagctat tagaaagcaa ttatttgata tttccactga
    24601 attagactct catcaattta acgaatttag aaattctatt cgtctatccg actatgaatt
    24661 ttactacaca tctaaatcaa atctaaattt ctgtatcaat gaaaattatg ataaattggt
    24721 aggaagactc aatgacaata tagctagtaa tcctgaagaa gacgatgaag ctctgttggg
    24781 ctcaagaatt gacatgtcag atattttcaa gcatatttcg gttgtttatg ttgacgctct
    24841 tcgagatgtt cttagagaaa tgaaaaacaa tcgaaaccca gtcaaaagaa tcatggaaac
    24901 aattgaatca aaaatatcat cagataatgt tgagtcatta aaaacaatta tacagcaatt
    24961 aaatgagact attaccagtg ttcctgaaat taggaaaatt ggcgaaaata ttaatcggca
    25021 actaaatagc ataattggtt cagtgtattc tcctaatctt cttctttcct caactatgtc
    25081 agatgatatg ggttctttag caaaatttct ttcaatgaag ccagaacaaa atatcgactt
    25141 agacttacta gggttaggac atctaaatat gatttattta gctttgaaaa tagtcgaatt
    25201 tgaagcgtgt cgttcaagag aactacttaa tattatgttg attgaagaac cagaagctca
    25261 tattcatcat catattcaaa aaactctgtt cgaaggatta aatcttcaaa aaaactatac
    25321 tcagattctc atgaccacac attctgtaca tttagctgaa gtgtctgaaa tatctcggat
    25381 gaatgtatta aaatcgttta atggcaaatc tatcgttatg aatccagcaa ataatctgaa
    25441 ctcatttgct aaagaaaagt taaaaaagaa caatctaaat ctaataaacg cagtagagag
    25501 atatttagat gttaaaagaa atggattgct tttttctaaa ggtgttatct tagttgaagg
    25561 agatgctgaa gaaatactta ttcctcaaat agtaaaaaaa ttatttggta taaacttaga
    25621 cgaacttgga ataggtttga ttaatgttgg tagtacttcg tttgaatata tcgctagctt
    25681 gtttgacgat gatagaatac agagaaagtg tgctattgta actgatttag atgaacaggc
    25741 aatcccatca gaacatcgat tgtataaaag cactgcacaa gagaaaggtc aagcaagaaa
    25801 agaaaaatta tcctcccttt ttaatgaaaa caattgggtt aattgttttt atgctaatac
    25861 aacatttgaa attgagtttt tagatgtgaa taaagaaaat attctaactt atgtcagtcc
    25921 aattatagat gacgcattca aagataaaaa aacaataact gcttataaag aagaaataca
    25981 aagtgaacaa tatttagaaa gaaatttagc gatgttaaaa ctagccaatc atgttggtaa
    26041 ggggtggttt gcaattgagt taggtgaaaa aattgacttt ttaatcaaaa ttcctaagta
    26101 tattcttaat gctattgctt ttgcatgtca ggaaatagta gatctctcaa tttataaaaa
    26161 aattctactc catacaatcg acttgtacga ggaaactaca gaattgtcag ctttatcagc
    26221 tagtttaaaa acaagtgata cagctgattt gaatatagca ataatttctt tattagagta
    26281 ctttggtaaa gatgaggatg ttgtgtatct aatcaatcag atagaaaatt acacaaaaat
    26341 actttatgct gaggtttcaa aatgacaaat atcttcgaaa aaatcgtaaa tgatttaaat
    26401 ttagaacaaa aagatgttgt ggaaagtgaa gataaccttt atgtaaatgc atgtcctggg
    26461 agtgggaaaa caagggtact tacaaggaaa attgcttacc aagcaattaa acacgatgga
    26521 tcactaaaaa gaataattgc tatcacatac actaatagag ctgctgaaga aattaaggaa
    26581 cgcctaagtt tacttagtat agatgatgat attaatattt gggtggggac aatacatcag
    26641 ttttgtcttg aatttattat ttatcctttt aaaatgaatc tctcaagagt atcaaaagga
    26701 tttacaatta ttgatgatta tactcaaaga gcttacgtca gccaaattaa taatatgctg
    26761 aatttaaatc tcaaaacgta tgaactgaac aaaattgatt tgtcactttc tcatgatttt
    26821 aaaattaatg aagttcagta tactcaagtt tctgaaatgt atcacactat gttggaaaaa
    26881 aataaagaaa ttgattttga tatgatttta ctagtagcgt ataaaatttt aactgtaaac
    26941 agtaacgttg cacgtaatat tgctagtaat ataagggcga tttatgtgga tgaatttcaa
    27001 gatactagag aattacaata caatgtaata gctaaattac ttcaaaacaa ccctcaaatt
    27061 cagtcaatgt ttgttggaga tattgaccaa gcaatttatg gatcattaga tggtatagct
    27121 aaatcagttg aagaactaga aatgctaact agtcacactt ttcaatcaaa gacgctacat
    27181 ggctgttaca ggtctaatca gagattagta gacttctact ctaattttca gagttgtcct
    27241 tacgagattg aaagcagagg gaataataaa aacgaccgtg gttttataag ttatgattgc
    27301 aaagttacta aggaggattt acccaacata ataaaaaata taataaagga aaagattgaa
    27361 agcggcattc atgaaaaaga tatttgtgta cttgctcctc aacactatcc tctgttttca
    27421 ctgggtgagt atttaaagaa agaattaccg tattgcaatt ttgatgctat aaatatatca
    27481 ccaattaagg taaataatca tagtttattt ttcaagttag caattttgta ctttactgaa
    27541 tcaggtgaaa aggttcgaag aagaaagcta attgcaaacg atattttaat tatattaaaa
    27601 aatgaatttg gaatagaagt taaagattat tttattgact tagatttatt acagctaata
    27661 aacagtgtga aaagaatgta tagtggagac gacaacggtg ttgatttata caaatttgtt
    27721 accaaacata tatttgttaa tcttaatatt gacgagaaaa attacccgtc tatttttaag
    27781 cactatctat tttttattaa cgaagtagaa gatcggataa aaaataattc attatctagt
    27841 agtgtatcta gttttaagaa gatgtttaaa tctcgagatg gtatcacact tactacagca
    27901 cataaagtta aaggaaatga gtatgatact gttatcgcta ttaatcttct aaacgggaaa
    27961 attcctcatt ggaatgaaat ttttaatgtt tctgataaag gaattagttc agcgagaaaa
    28021 ctactatacg taatttgttc aagagcaaaa aataatttat ttcttttctc agaggtgggt
    28081 tattttacga gtaaaggaga cttgctttat cccactcaat tattagataa aatagaattt
    28141 gattataata tttaaaatac ctaggtagtc gttgttttaa atatagtagg aatcggacgc
    28201 tcagtacgga gcgtctattt ttttacccaa ttttgaaagg aagtgaactt atgaaaataa
    28261 aaaatcaaga atcaaaaggt cgctctcccc cctttaaaac catcacacaa ataagattca
    28321 ttcaaacata aaaacagaaa ggataggtaa aaatatggaa cttaaatatg tcattcccaa
    28381 tatggaaaaa acatttggac aattagaata tgctggtgaa gggaacattg aacaacgtcg
    28441 aataaatggc agaccaacca ttctctctcg tagttacaat ttatactcaa cgattcaacg
    28501 agcagatgat attgttgtca ttcttccctc cgaagctggc gagaaacatt ttgaatccga
    28561 cgaaaaagtc aaactcatca acccacatat taccgcagaa ggttataaaa ttggcaatcg
    28621 aggatttacc aattacatta tgcacgcaga cgatatggtg aaagcataag aaaggaaagg
    28681 tgaaaaaata tgagattagc agaaggtatt gtgattgaaa aagaccagac atttggcaca
    28741 ttgaaatttt ccgccttgcg tcgtgaggtt cgtatccaga atgaagatgg tacggtttcc
    28801 gaagaaatta aagaacgtac ctatgactta aaatcaaaag gacaaggacg catgatacaa
    28861 gtcagtatcc ccgcttctgt tcctttgaaa gaatttgact acaacgcaga agtggaatta
    28921 atcaatcctg tggcagatac cgtagcgacg gctacttttc gaggggcaga cgttgattgg
    28981 tacgtgaaag cagacgatct tgtattaaaa ggacagacaa aacctcatca gaaatataaa
    29041 aaagaagcgt ccgaaagtga aaaataggtg ttgcttatga caaacacacc attgattttg
    29101 aaagaaattc cgaaggatga agccatttct tttattcggc aataccatta ttcaaagata
    29161 ctccctagat tgtgtaaata ctttctaggg attttttcag aagaaaaatt attaggcgtt
    29221 gtggaattag gctggggtac gcaaccccta caaaccatac gcaaactctt tcccgacagt
    29281 tcccttcaaa ccacagatta tttagaaatt ggaaagatgt gttttcttcc agaaatgaat
    29341 caaaccaatt attttggcag tcaagctctt tcagctctaa ttaagtggtt gaaagaacat
    29401 acggactgtc attttttata tacactggca gatggaattg agggaaaatg tggctatgtc
    29461 tatcaagcgt ccaacttctt ttactgcggg tatttcaaga caagtgtcta tcgtgataag
    29521 cagtcatggg aaaagataca ccctcgaagt gctcgccttt tattagagga aaatgctcgc
    29581 tttgaacagg tagagaaaaa gcattggctt tcccaggcat tttgcgaata caaagggatt
    29641 gagaaaatca atggacgcat gtttcgctat ctctatccgt tgacaaaaga agcgaaaaaa
    29701 ctgttaggac acactctcta tcgacgtcat tattatccga aagaaaaaaa tttacgcttt
    29761 gaaaagcgaa tcgcttatca gaaatatgaa gccatttctc aaccaacttt tgataaacag
    29821 gctcgtatct acaatacaca attattttga taggtggaaa ggaggatttt tatgccattg
    29881 cttcatgaca gaggagagcg aatcaaagcc aacgataagc acttggtcta tcattttgta
    29941 ctaatttcat tgctggtagt ttttctagtc gtcctactcc cttttcattg gagagcgtta
    30001 caatcggtga actggcaagc agttcagtta gatacatttt tctatcaact tcatacgcca
    30061 tacttttgga gcagtattct cactgctctt tttgtatgcg gacttatcag ctggctttat
    30121 tatcactatc gtatagattc tataaaacag ttagagcacc gtcaaaagtt agctcgtatg
    30181 attttagaaa atcattggta tgaagccgaa gaaactcaat cagagggatt ctttaaagat
    30241 ttacccagca agaaaaccaa acaacgaatc cgccattttc ctaaaatata ttatcgaatg
    30301 aaagatggac taatccatat tcatgtagaa atcaccttag ggaaatatca agatcagctt
    30361 ttgcacctag aaaacaaact agaaacagga ttgtattgtg aattagtgga aaaaacgttg
    30421 aaagattctt atgtagagta tgtcctactt tatgatacta ttggcaatcg aattggcatt
    30481 gaagacgtgg tagctcaaga tggacgtgtc aaattgatgg aatcggtcta ttgggagttc
    30541 gattctctcc ctcacatgtt aattgctggg ggcactggta gtggaaaatc ctattttatt
    30601 ctcacattga ttgaatctct cctgcataca aatgcaaagc tttatattct tgaccctaaa
    30661 aatgctgatt tggcagactt aggggcggtc atgaataatg tctattaccg taaagaagat
    30721 atgttggcat gtatcaatca attttacgaa gatatgattg atcgcagtga aaccatgaaa
    30781 caacacccta actataaaac aggagaaaat tatgcttatc ttggtttgtc tgccaatttt
    30841 cttatttttg atgaatatgt ggcaatgatg gatatgctag gtcgtgaaag taccaccgtt
    30901 attcataaac tcaaacaaat tgtaatgtta ggacgacaag caggcttctt tttaattctt
    30961 gcctgtcaac gtccagacgc aaaatatttt agtgatggga ttcgggacaa ctttaacttc
    31021 cgtgtggctt tgggtcgtat gtctgaaatg ggctttggta tgatgttcgg aagcgataca
    31081 caaaaacaat ttttcttaaa acccatcaag ggaagaggtt acgtggatac aggaaaaagt
    31141 gtgattagtg agttttatac accccttgta ccaaaaaggt acgatttctt aggagaaata
    31201 ggaaaagtca ttcaaaaaaa gcaatctgag cctgtaccgc acgaagtgaa aggtacaggt
    31261 tcagacttgc tttgattggt gtggcgttag ccacgccaat cctttagccc cctctatcta
    31321 acaggggggc acaaatacaa ctgaaaacag gcaattctca tcaaccaatg ttgatggtac
    31381 aaaggattgc ggaaaatatg aaaggtgtaa actcaagggt catagtttac atctttttta
    31441 gattggaggt ttgcgaatgg aaaacgaaaa aatttggatt aaggaattga aagaaaagcg
    31501 actggcttat ggcgtttctc aaaataaact ggcggttgct tctcatatta ccagacctta
    31561 cctatcggat attgaaacag gcaaggcagt ccctactcaa caggtgaaag aagatttact
    31621 gaacgctttg gaacgcttca atcccgataa cccattggag atgttattcg actatgtacg
    31681 gattcgattc cctaccaatg atgtggctca aattatcggt gaggttttac gtttgaatat
    31741 ggattatatg ctacacgaag actttggcta ttattcttat cccgaacact atcgtttcgg
    31801 tgatattgtt gtcctagtct ctcatgatgt aagtaaaggt gttttattag aactgaaagg
    31861 aaaaggctgt cggcaatttg aaaactttct attggctcaa catcggagct ggtatgattt
    31921 ctttcaagat tgcatggaac ataaaggtat tttcaaacgc ttggatttag ctatcaacga
    31981 taaaacaggg attttgaata ttccagattt ggcaaagaaa tgtaaacagg aagaatgcat
    32041 ttccgtcttt cgaagcttta aaaattatcg cagtggtgaa ttggttcacc gtgatgaaaa
    32101 accatacatg ggaaacacac tttatattgg ctcattaaaa agtgaagtct atttctgtat
    32161 atatgagaaa gattatgagc aatacgtgaa aaatgatgtt cccttagaag acgcagaagt
    32221 aaagaatcgg tttgaaattc ggttaaaaaa tgaccgagcc acccaagcca tgaaagattt
    32281 attggcacat caacaggcgg aaaaaacagc ctttgaaatt atcaatcgct atatccgctt
    32341 tgcagataaa gacgacacta aacgtcggag tgattggaaa actaatgaac gttgggaatg
    32401 gtttatcggt aaaaatcgtg gagcattacg cctaaccaca cagccagagc cttattcttt
    32461 tgaacgaaca ttgaactggt tacatcatca agtcgcccct acattaaaaa tcgcaagcat
    32521 tttagatgtt ctcaacggta caaccattat ctccaccatg attcaggaag ccaaactgac
    32581 agaaaaacat gagaaattaa ttgaacaaca acatctagcc atggaggatt tgattacatg
    32641 agccttttaa caaaagaatt aaagaagtta gggtttcagt gtggcattga gtttcaagcg
    32701 tatatacaaa atacagggaa atatacgtct ctcattattg agggaaaacg gcaagctggt
    32761 gataccatct atacctatga tttttacaag gtgagatttt ataataatta taccaatcga
    32821 gtaaccgtct atggagagca tttaacccct tttcaattat taaggcgtgt aaaaagctat
    32881 atctattatc gagaaaaata tcttaaggaa aggaggacaa tcacatgaat tttgggcaga
    32941 atttgtacaa ctggttcttg gataatgcac agtcattggt attgttagcg attgttgtca
    33001 ttggactgtt tttaggtttt aaacgagaat tttcaaaact cattggcttc ttgattattg
    33061 ctctaatcgc cgtcggactt gtgttcaatg cagctggcgt gaaagatgtg ttattaaact
    33121 tatttaaccg aatcattggt gcttaacaga tatttttcat gataaaatag gaagtatata
    33181 ttttgttatg aaaggattta tgaatatgtt tacaaatgat caatttaaat ctcttttaat
    33241 taaaacatta gaaagaaaga gctcttcaaa ttattatgaa ggaggtaacg agattgtttc
    33301 taaaatgaaa atatcagagg taactttaga tgagactgac gattttactt attataaagg
    33361 atataaaaag ggctggaata cgctttgcac atatcttaaa ataagagttc cttttgatga
    33421 tttagacttt tttgaaagtc ataaagattt gattacccag acagcaagct ccatatatga
    33481 taaacaaggt gataatgtat tagtggatac tattttagta ccacttccag agaattacga
    33541 agtaattaac tttagtcagt tgaaaattag tgatgtggtt tcacaagcta ttgaagacgc
    33601 tgagtctttt atgagtaatg gtgaatatca acgtgccttt gacagagttc acactgcttt
    33661 tcatggatat ttaattgaaa tactaaaaaa atatgaaatt acagttccta gagacgaaaa
    33721 tctatctaaa ttatatagta ggatacaaca attaattgaa aaagaaattc aacctactga
    33781 acttgctgat atagtgaaga caacgataag gtcatcaaat ggaatgatta gctctcttaa
    33841 tgaagcaaga aataggcatt cacttgcaca ccctaataca aatattattg gaaagagaga
    33901 agcaaaacta ataataggta tttcaagtac agttacagat tatattagcg gataccttga
    33961 taagtagcgt cattaactga ttgctacttt ttttatgact atttttataa aaaggatgtg
    34021 ataagaattg gaaatgaaag tctatgtggc aaatttagga cggtataatg aaggtgaatt
    34081 agtcggagca tggtttactc caccaattga tgaggaagaa atggcagaaa gaatcggttt
    34141 aaatgaggac tacgaagaat atgcaataca tgattttgag cttccttttg atgtggacga
    34201 atacacgccg attagtgaaa tcaaccgtct atgtgaagcc attcaagaaa tcgaaggtac
    34261 acctatttat aatgaactca aagaaattca aggcatgtgg tttagtagct tggaagaatt
    34321 actggaaaac aaagaggata ttcattgtta tagtgattgt gattcgatgg aagatgtcgc
    34381 ccgttattac gtggaagaaa caggacagct tggtgaagtc ccttccaatt tacaaaatta
    34441 tattgactac caatctttgg gacgtgatat ggaaatagag ggtaattacc tagttacctc
    34501 tcatggggta tttgaatatt gtcagtaagt ttaacaatcc gtgttataat taaattaata
    34561 aatagcacgg agaggtataa attatggtaa atgtatttga aaatcttaaa ctattaaaac
    34621 tagacatgaa agataaaggc tgggtgatag attcctttta ctttcgttac aaacagcaaa
    34681 attatattgt acttgttaag ctatttgaaa aagaggaaaa agttccagaa tatgctctgt
    34741 taaaactaga atttttaaag gaaaacgatt tttcagatat gctagctgtc tatgctaatt
    34801 cggtaaaatt atatacagac acgaaaacaa taagagaata ttttggtatc gaatacagta
    34861 gtaacttggg agatgtttta tttcaattta gccaaactct tgctcgattt attccgacag
    34921 aagttagtga gaagaaaaat gaagaccaaa aagaagccat gtgtttctca ttaagtcaaa
    34981 gtgattcaga agatcccaga aaaaaatatt gtttttcagt tagaagaaat cctttaaagg
    35041 gaaatggtga gctgggaaaa cgaagtcctt ttaatgacaa taaaacgaga ttgtatagac
    35101 ccagcttata tgaacgatta gggtcagata ccaatttaag tttcagatat tcaatgaatc
    35161 cagatgatga agaaactgat gaaggtatta ttgcaaaatg gacaaaaaat aaaattgaat
    35221 agttaaagca ttgaaagagc aatcacctat tttgattgtt ctttttctat ttttacggaa
    35281 aggacgtgaa gacatgaaaa aaataagaag ttacaccagt atttggtcag tagaaaaagt
    35341 gatttatgcg attaatgatt tacaactccc tttcccaatt acctttacgc aaatgacatg
    35401 gtttatcgtt tctttgtttg tcgtcatact ctttggcagt ttaccaccac tttctctgat
    35461 tgacggagca ctactcaaat acttagggat tcccattgtt ctcactgggt tcatgtgcca
    35521 aaagaccttt gacggaaaga aaccctatgg atttctaaag tctatcatta cctatgctct
    35581 acgccctaaa gtaacctacg ctggcaaaca aatacgatta aaaaaagaaa caatgaatga
    35641 atcgattacg attgtaagga gtgaacaact agatggcata tccgattaaa tatatggaag
    35701 ataatctcgt atttaatcat gatggcgaag tattcgctta ttatgaatta attccctata
    35761 attacagttt tttaagttcc gatgaaaaaa ttcaagttca tgataatttc tgtcaattga
    35821 ttgcccaaaa tcgtgatggc aagattcatg ctctgcaact agcaaccgaa tcaagtattc
    35881 gagccacaca ggaacaatcg aaaaaagaaa tcactggacg attaaaagag atagcttacg
    35941 aaaaaataga tgaacagacc gaagcgttaa tttctatgat tggtgacaat caagtagact
    36001 atcgcttttt tattggtttt aaactctacc tcaatgaaca ggaagtatcc gtaaaaaatg
    36061 ttgggaaaga aattcaaaaa gcatttgcgg aattctttca tggtgtgaat cacaaattaa
    36121 tgggcgattt tgtttctatg cctatggcgg aaatcgaacg cttttctaag atggaacagt
    36181 tgttagaaag taaattatcc agacgctttc agattcgtaa attggagaaa gatgattttg
    36241 gttatattat tgaacatctt tacgggcaaa caggtgtccc ttttgatgac tacgactacc
    36301 atttaccgaa acgatacgaa gataaaaaag tgttggtaaa gaaatacgat attctcaaac
    36361 ccactcgctg tttaatcgaa gaaaatcaac gctatttacg gattgaacag gaagatacca
    36421 ctacttatgt ggcgtatttt acaattaata gtattgttgg tgaattggat ttccctaata
    36481 gtgaaatttt ttattaccag caacagcagt ttactttccc cgttgatacc agtatgaatg
    36541 ttgaaattgt cgccaataaa aaagccttat ctaccgttcg caataaaaag aaagaactga
    36601 aagatttaga tagccacgca tgggaaagcg acaacgaaac cagtactaat gtggtggacg
    36661 ctttagaatc tgtcgatgaa ttagaaagta atttagacca aaccaaagaa tctatgtaca
    36721 aactaagcta tgtggtacgg gtatcagcaa agaatttgga cgagctgaaa caacgttgtg
    36781 atgaagtaaa agatttttat gatgatctgt ctatcaaatt agtacgacct tttggcgata
    36841 tgctaggctt gcataatgaa tttattccct caagcaagcg atatatcaat gactatattc
    36901 aatatgtcac gtcggatttt ttggctggtc ttggtttcgg tgctacacaa atgattggtg
    36961 aaaattctgg tatttacgtc ggttacaatc tcgatacagg cagaaatatt tatctccaac
    37021 ctagccttgc aagtcaagga attaaaggct ctgtaacaaa tgcattagcg tccgctttta
    37081 ttggttcatt aggtggcgga aaatcattct ctaacaatat gctggtctat tattcagtac
    37141 tctttggcgg acaggcaacc attgttgacc caaaagc
//
LOCUS       DASHRQ010000022        36818 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.022, whole genome shotgun sequence.
ACCESSION   DASHRQ010000022 DASHRQ010000000
VERSION     DASHRQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.022"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            379..1197
                     /locus_tag="U2M88_001228"
     CDS             379..1197
                     /locus_tag="U2M88_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEM8373620.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
     gene            1216..1623
                     /locus_tag="U2M88_001229"
     CDS             1216..1623
                     /locus_tag="U2M88_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373621.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            1943..3028
                     /locus_tag="U2M88_001230"
     CDS             1943..3028
                     /locus_tag="U2M88_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEM8373622.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHKAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            3865..4197
                     /locus_tag="U2M88_001231"
     CDS             3865..4197
                     /locus_tag="U2M88_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEM8373623.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            4788..5117
                     /locus_tag="U2M88_001232"
     CDS             4788..5117
                     /locus_tag="U2M88_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373624.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            5104..5445
                     /locus_tag="U2M88_001233"
     CDS             5104..5445
                     /locus_tag="U2M88_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEM8373625.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            5559..5798
                     /locus_tag="U2M88_001234"
     CDS             5559..5798
                     /locus_tag="U2M88_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373626.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            5865..6104
                     /locus_tag="U2M88_001235"
     CDS             5865..6104
                     /locus_tag="U2M88_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373627.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            6231..6920
                     /locus_tag="U2M88_001236"
     CDS             6231..6920
                     /locus_tag="U2M88_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEM8373628.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            6926..8092
                     /locus_tag="U2M88_001237"
     CDS             6926..8092
                     /locus_tag="U2M88_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEM8373629.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            8174..8845
                     /locus_tag="U2M88_001238"
     CDS             8174..8845
                     /locus_tag="U2M88_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEM8373630.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            8898..10874
                     /locus_tag="U2M88_001239"
     CDS             8898..10874
                     /locus_tag="U2M88_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEM8373631.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            10887..11639
                     /locus_tag="U2M88_001240"
     CDS             10887..11639
                     /locus_tag="U2M88_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEM8373632.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            11655..12416
                     /locus_tag="U2M88_001241"
     CDS             11655..12416
                     /locus_tag="U2M88_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373633.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            12429..12689
                     /locus_tag="U2M88_001242"
     CDS             12429..12689
                     /locus_tag="U2M88_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373634.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            12686..14776
                     /locus_tag="U2M88_001243"
     CDS             12686..14776
                     /locus_tag="U2M88_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEM8373635.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMNKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(14812..16869)
                     /locus_tag="U2M88_001244"
     CDS             complement(14812..16869)
                     /locus_tag="U2M88_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEM8373636.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(17014..17205)
                     /locus_tag="U2M88_001245"
     CDS             complement(17014..17205)
                     /locus_tag="U2M88_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373637.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            17393..17848
                     /locus_tag="U2M88_001246"
     CDS             17393..17848
                     /locus_tag="U2M88_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373638.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            17845..18087
                     /locus_tag="U2M88_001247"
     CDS             17845..18087
                     /locus_tag="U2M88_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HEM8373639.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            18105..18407
                     /locus_tag="U2M88_001248"
     CDS             18105..18407
                     /locus_tag="U2M88_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373640.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            18476..20530
                     /locus_tag="U2M88_001249"
     CDS             18476..20530
                     /locus_tag="U2M88_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373641.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKHTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            20530..23142
                     /locus_tag="U2M88_001250"
     CDS             20530..23142
                     /locus_tag="U2M88_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEM8373642.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            23139..25517
                     /locus_tag="U2M88_001251"
     CDS             23139..25517
                     /locus_tag="U2M88_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373643.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            25578..26120
                     /locus_tag="U2M88_001252"
     CDS             25578..26120
                     /locus_tag="U2M88_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373644.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            26198..26530
                     /locus_tag="U2M88_001253"
     CDS             26198..26530
                     /locus_tag="U2M88_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373645.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            26531..27124
                     /locus_tag="U2M88_001254"
     CDS             26531..27124
                     /locus_tag="U2M88_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373646.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            27146..29170
                     /locus_tag="U2M88_001255"
     CDS             27146..29170
                     /locus_tag="U2M88_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEM8373647.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            29170..30369
                     /locus_tag="U2M88_001256"
     CDS             29170..30369
                     /locus_tag="U2M88_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEM8373648.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            30385..31029
                     /locus_tag="U2M88_001257"
     CDS             30385..31029
                     /locus_tag="U2M88_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373649.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            31040..31846
                     /locus_tag="U2M88_001258"
     CDS             31040..31846
                     /locus_tag="U2M88_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373650.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            31869..32098
                     /locus_tag="U2M88_001259"
                     /pseudo
     CDS             31869..32098
                     /locus_tag="U2M88_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32119..33192
                     /locus_tag="U2M88_001260"
     CDS             32119..33192
                     /locus_tag="U2M88_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HEM8373651.1"
                     /translation="MKKTYQKFLWISYIVLFIVGFLVSVSVIVGTLFLLFSDEDLVLK
                     GLMSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIEYTWLPLKRNSKR
                     KEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQIAASNIFTAVMTL
                     MCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIK
                     NFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVD
                     FGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYNFYKVEASLRYGI
                     GKLRLDGSNIQQNKPYVLGKVVI"
     gene            33236..33505
                     /locus_tag="U2M88_001261"
     CDS             33236..33505
                     /locus_tag="U2M88_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373652.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            33781..34056
                     /locus_tag="U2M88_001262"
     CDS             33781..34056
                     /locus_tag="U2M88_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373653.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            34098..34520
                     /locus_tag="U2M88_001263"
     CDS             34098..34520
                     /locus_tag="U2M88_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373654.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            34540..36132
                     /locus_tag="U2M88_001264"
     CDS             34540..36132
                     /locus_tag="U2M88_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373655.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            36153..36488
                     /locus_tag="U2M88_001265"
     CDS             36153..36488
                     /locus_tag="U2M88_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373656.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            36634..>36818
                     /locus_tag="U2M88_001266"
     CDS             36634..>36818
                     /locus_tag="U2M88_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373657.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT        13378 a         5612 c         7349 g        10479 t
ORIGIN      
        1 ataaaatcat tacctgcttc atttgtatat gatctaagtc ttgcttctag tatgaatatg
       61 accgatgtat acgaaaagtt attaatacta gaaaatgaat atgatcaatt caattcaaat
      121 cagcagcgaa aagtaaagaa acagatagac tagaatagct attttcgaaa agcaatattt
      181 aaggtataag ttataccttt aataataaaa gtataagtta taccataaat atatatggta
      241 taacttatac ttttattatt aaaaaccgct atatccaaac gtttttagat attattattt
      301 aagttatact tttaatgttg accatttccg atatataagt tatactttaa ttatcaatta
      361 aagattggga ggatttacat ggatactttt caagggacta catttacagt tggcaatttt
      421 aaaggaggag taggtaaaac taagatagtc agcatgcttg cttatgacaa tgcaatgata
      481 agaaatagaa aaacgcttat tattgattta gaccctcaag cgaacttaac acaagtcata
      541 gcaagaactt tcggtataac cgatatcaaa accactataa caaatggtgt cgcaaatgga
      601 gaattagcaa gtagtattat taaggtaaat gaaaatcttg accttctggc atgtgacact
      661 agttttcgat cattctccgc ttttgctaat aaacatgaaa atgaattaga ccaagttact
      721 gtcttgaaaa aattacttct tccaataaag aacaattatc aagaaatatt tattgatgta
      781 ccaccaacca ttagcgaatt ttccgataat gctatggcag cttcagacta ttcgataatt
      841 tcatttcaaa cagctgaaga atccttagag ggagtcaaca aatatgtaaa ttatcaaaaa
      901 ttcatggtcg atagatacga tcttgatttg caaattatag atattgtacc ttgtatggta
      961 gaaccaaatg atgattttga tgaagaaatc ctagaagaag caattgaaaa atacggaacc
     1021 gtggtatcca aaaatctcat tcattatcaa aaaagattga gaaggtattc taaaattggt
     1081 atacatttaa gaaaatacaa aaatggaaat tttgatcaat gggattataa agcacaccaa
     1141 gtatttataa atattttggc agaactagat gctagacaag agtttttaaa taaataataa
     1201 ggagttgcat aagtaatgcc taaagatttt gatattccta aaatgatgga gaatggtgaa
     1261 tttgagccat tattacccaa taacaaacgc caagatgtga aaataagcgg tgaatataac
     1321 tttagcaata gtgcattgag taacacaaga aatattacat tgaaaaaaag aggccgacca
     1381 gtttcaattc ctgataaaag aaacaaggta tcagttccta aaaagatctc tccggcatta
     1441 aatagcaaac tctcaatttt acaagactat atgacggagt tacaaagtga aaaagggaga
     1501 atttctttcg aaaaaattgt taatacatta gcagactcat atatccagca tagactagga
     1561 gtagcaaaag aagaacacat aagagaagaa atacaagaag agtttgaaaa attaccaaaa
     1621 taattttttt tcatgcggta ttcaattaaa gtataagtta tactagataa atgaatatta
     1681 aagtataagt tatacattaa atatttaagg tataacttaa taaacaacgt gtaaatttgc
     1741 gattaaatca acattataaa attattattt aaagtataag ttatacatta tttgtattta
     1801 aagtataact tatacttatt tttttaatat ataacttata ccttaaatac tgaaagtgtt
     1861 ttattcaccc ttaatacatg gtatagttca cttgttgaaa atcctgaata tatacaagtt
     1921 tctaagaaga aggagaaatt taatgtctac agaaaatcaa tctcaaaaaa tgataattaa
     1981 aaattgggga tataaagcaa cgaatgttta tattgaaaaa tattataaat ttcctagttt
     2041 tttaattcag aattctaaat actctaaaat gaaagacagc tcaaaaatag cctatatgct
     2101 ttttaaaaat gaatttcgta gagccttaca tgaaaaatgg atcgacgatg atggtctatt
     2161 atatttggaa tttacacaag cagacctaat ggacaaatta aattgctacc aaggcaaagt
     2221 aaaaaccatt ttaaacgagt tagaaacata taatttgatt tcagtcgtaa aaggagaatt
     2281 taacccaaaa acgaaaaaaa atgaaaaaaa taaatactat ttacttcaac ctgaaataag
     2341 tgccgatgat ctttttattc aaaattcttt cgatgacgat ttaagcgaaa ctattgataa
     2401 atcaacctct tctcggaatg cggaaatcgc acgtcgagag gaaactgcta aaacccttga
     2461 taacactgga aatgcggaaa tcgcacgtcg agaaaaaaca gctgaaaccc ttgataacac
     2521 tggaaatgcg gaaatcgcac aagattatta tattaataaa tctttagaca ctaaaagaca
     2581 cttaaaagac actgaaacag accaattaca aaatcaagtt ttgctagata acttcgttga
     2641 aatcatgaaa gaagacagca taaacacatt tgtaccagaa aaggtattaa gcttgataaa
     2701 aacattttct aacacttacg aagaagccca acaaaccgtc accacaatac acaaagcaaa
     2761 acataaagcc gaggaacttg agaagacggt agttgtgtat gaagaacttg aatcttacgg
     2821 aattgatgcg gataaaggat tatatatgac gttgctgaaa gcttatcaaa aacaaaagac
     2881 tgagaaagta aataatttgc agaatttgat ttttgtgtat gtaaaaaact ggtttattga
     2941 aaaagctatt gcggcaaagc ttgcaaacga acaaagagaa acatttaacg aattaccaac
     3001 agttagtacg gaaaattggc tagagtagtt gataaaaagc gaacactata aaaatgatca
     3061 attttaaatt agaacttttt tacaatttta attttattac aaatgttaca aataaatagt
     3121 tattattccg gagtttttag attaaaagtt tatgaaaaca atcacaaacg taggttttaa
     3181 tgtcataatc gttagatatt ttagttattt ttcattcaaa aaaacaggga actagtgttt
     3241 gcatatgtat ttttttgagt tataataatt catgaagaga ggtaattcac ttatttaccc
     3301 aagctgaaaa gcctgatatt acttcttttc aggcagtgct gctaatattc aattacactt
     3361 ttttgtataa atttagttgt tttaaattta attgtctgct atgcttgtga tgaattgatt
     3421 ttcgttctgc acttttggac gataaatttg gacaattgaa aatacaaaaa aaagaaaggc
     3481 cctgtgagac tcggaaggct accaaccttc gtcgacgtcc acaggtggca ttaagtctat
     3541 accccttaat gcgtttacct ttcagtgtta cggtattacc cgtaacggat ttagtataac
     3601 agatattcaa aagtgatacc actttttttg gctgtgttaa ggttaaaaat acgtgatatt
     3661 acgatgaatc tatgcctaag cataatgtta taaaaatgtc cgtggggctc tttgccgtat
     3721 gacgaacgct gaaattagag ggaacgcatg tttgaatgcg ttccctcttt tttgtttgtc
     3781 atgaacgaaa atcaattctc tatttttgaa aaataacaag aaaaacttgt taaaaatatt
     3841 taaagtaagt gggtggatta ttttatgtca tatacaggaa ttttgagtct cgaagatatt
     3901 tgtcactacg gtaaacggtg taccgctaca gaaaagatca cgaagaaact atcaactggt
     3961 caaagtaaaa ccgttgtgca gtgtaagaaa tatataatcc agaaagacaa agtctcagaa
     4021 gaaatgattt attacattgg gaaacaaaaa caaataattt taaaagatcc tatcccttta
     4081 aaagagctat atccaacaat caaacatgtt tatgaccaaa acggtgtact gattggtcga
     4141 agaaaaaatg gagtcttgcg ttgtaccgca aaagggatgg ggcgtttaat cagctgaatt
     4201 gatcattctc ataacttgta ttttatcgtt tttcgcttgc ttgaaaatat tgtcgagact
     4261 tctaaaggaa cttagaatca ctagaaaaat aaggagtaga cttgatctat caacaattcc
     4321 cttcggggag tgacggggta ggtttttacg attggtaacg taaaaaattg gtcaatcgag
     4381 taaatgccct tgtttggata gtggtgaaat gaatttgtgt aaataatatg cacagagtaa
     4441 acaagaggga atttagaagt tagacaagtt agaatagcgc ctagattaat catgagcagc
     4501 tactaaggga tctaggcttt gacgcaaact catggaataa ggagtaggtc ttctcgtaat
     4561 ggggaggacg cacaagaaat tgtgtcgtgt gctgcagtac atgtagatac ttgcgaggac
     4621 gttgatcatt ggatcaatcg agcatttacc gctgtctact agggtttctc gtttcggacg
     4681 ccataaacga gtcgatttat tttatatatt tttgagtgaa cgaaaaacga taaagatatt
     4741 cgttatgaag taaaactaat ttagtagata gcaaaggtgg tagaggaatg attcaaaaat
     4801 ttgcgaaaat gagtaaaact gcatggattg aaaagcttga aaatgacttg agtgtcatgc
     4861 gattagctcc aaagaaattc aaacgagaga ttgcagaaaa agaagcaatt ttgtcggtat
     4921 tgaatgcggt agacgataaa tgtccagtaa aaattagttt ttcagatata gcaacaatta
     4981 tcgaaggtat ttattatggt gaagttgatg aagagatatc ttctacaaaa gatttattag
     5041 attttttgga taaaaacaaa gataccttta tagtactaac tgattatgag gaggttacaa
     5101 caaatgaaaa attatgatcc gaatatacga ttgggcacac atacgattaa ggtttctttc
     5161 caacgttggg attacaaagg atttgtgacg tttagaagag gcggaaattg taaaggatta
     5221 gatgttttag cattagacga ggatgattta tacgatcaaa cattgactga taatccaatt
     5281 ggattcggct tgctacctga agatgatgaa ggtaatgaat ggtttaaaat gacgcttacg
     5341 gatgataatg gagacgaatt atctgttgaa gatacttggt catatttgag tgattatatt
     5401 gtcagcgttg aaatcattga atttgtagcg gataaagaag aataaaaact ggaggaataa
     5461 attttacaga taaaggctta gaaggagtca cgacgaataa taaaaaggta gtattacctt
     5521 aggcattgag taaggaattt atttaggagg aaaattagat ggcgcgacca gttttagagg
     5581 cgatggatat ggatactttg acaaaaggaa ggtatgtaca gctgttagct cgtggttatt
     5641 cacctaaaga tgtattcaaa tccctgtcta aaggaacaga tatggaaaaa gaaagattac
     5701 gaaaagaatt cgattattgg agaacccata atggaatcaa ggatttgaaa ccagcacggc
     5761 cagtaattaa acgaaagaaa aagataaccc aagactgaag tgtagagcgt atgtgatcat
     5821 aggctttttt atttttgcaa aaaacaagaa aggtaggaaa actcatgaat tatgaagaaa
     5881 tcgaaaacag aaaaaaagtg tcgaaagaaa tggaagaaaa gttgctaaaa atgatgaaac
     5941 aaaaacacct taaaagatta tctgtcatgc aatatatcaa tgatatgaag ataacaggaa
     6001 aagagaaagc ttgtttgtta ggttcaatga aaaactttga acagttgagg cgaacttatg
     6061 taaaaacagg ttccaattgc caactattat tagaagtttc ttgacgatta tgatatgaaa
     6121 atgatcaatt atgatgtggc attgttattg aataaaaaga atagtaaaat ttaacatggt
     6181 ttatcttggt tagatgtagg taaattaatt catatataga aggtagatag atgaataaaa
     6241 acaataaaac tttatttaat ccttacgtgg gcgtaatcat tgtttttctt ctttttagtt
     6301 attttgctta tcaaggttat caaaaggaca aaatcgctga aaaagtagat ggtgtaacaa
     6361 aaacaatcca tattcaggaa caggctaaaa cctatcaaga taatttaacc agtttaacgg
     6421 atatttctaa gagacaactg attactaatc aaaaacaggc attagaaaag gatctcaaag
     6481 gtgaaattgc tggaaaactt gttttttctt ctacaggatt tattctccct gtttttcaag
     6541 gagcgaatag cgacacgctt agtttaggag tagcttctac ttattatcta gatactgaaa
     6601 tgggaaatgg aaattatatt cttgctggtt ataacgtaga tacaccaagc gttttattat
     6661 ccgatatgac tgatttatct gaaggagaag aaattgatct aatcgatgct cattacaagt
     6721 atcagtatga agtctccaaa agaggaaaaa tttctgatta cagccaggta gttcaatcta
     6781 aggaaaacaa ttccttttta tccttgccaa aagcaggtaa aaaaccacta ctcacactgt
     6841 tagcctttca agcaaataat atgaaaaagt gcgatgtgat ccagtgtgaa ttagtcaatg
     6901 tagaaaaaat caataaataa gggaagtgag tcacattcaa gaacttgtag aaaaattgat
     6961 caataaaatg agtgccattt ttctgttact gttggtatcc attctatatt taatatggac
     7021 ggtggggtta aatcctccta cgatattatt tatttatgat aatgctgcac agttagtgac
     7081 gtttttcgta ggctgtttgt ttcttttgaa tctgtacaag gtaaagagaa acgatctatt
     7141 cttcattgca gcaggcattc ttttttacct tttcttttct ttttttagaa gctaccgagg
     7201 agatactttc tatggtgatc tgatcgttcc gacaattatt gctattattt tgattgtaaa
     7261 atggacgaaa tttactgtaa ttgatcgtag cttgtatctt gtagtattct atgtcttttt
     7321 actcgtaacg gtttatcgag tatttactga gataccagta ccagaaggac aaagtatttg
     7381 gaatccatcg aacaaattat cagatatttg gatcaataca aatacgatag gtagttcttt
     7441 aatgacatta gcccttctta tcacaggatt tgctagttca tttggaaaat ggtacatgcg
     7501 attgctatca ttaccagcgt tgattcttgc tggggcaacg atttgggttt gccaatcgaa
     7561 agcagcatta tatgcgcttg ttatcttcat tatcttagaa attttaccta agaaggtttt
     7621 tcgaaataca cacttgtcct tttggatgta tgcagcagtt tcggttctag ctttaccgat
     7681 ttcttatttt gctgcaatgt cagacaaagt tcacttattt acaggacgag aagaaatttg
     7741 gcgcaaattc tatgaaacat taggcgaaaa aacaggacaa atatttgtag ggatgaaacc
     7801 tttctttttc catagaggaa atcagattct aggtaatcat aatacgtatc acgcactcgt
     7861 caatcactat ggattgattg gttttgttct atcgattatt cttttgctta gtttcttatg
     7921 gttgctcgta agaaaacgtg agctgacaaa tggacaacta acattcttgt gggctttttt
     7981 agcgatcatg tgtcaatcgt tcatggaaga aacattaacg acaatcagtt gggtgccaat
     8041 tgtattctta atgttagcaa tggctacaca ccaatataaa accaccagat aaatgaaata
     8101 gaatagtaat aagagaaaac actttttcgt tgtgaaaaag tgttttttgt tttgtctgaa
     8161 aggaggcaga tggatgaaaa aatggttatt tggtttttta ggagtcgctt taatcgtcgt
     8221 ttgcagtgtg tttggctatg ttagctacca aaaacatgaa ggagaggtgt ttaagcaaaa
     8281 tatagaaaaa aagatgcccg tagatcaaat caatgcacac gcaaagagtt ataaggagga
     8341 tgcaacgaac gtcaataatg atatgtcttt ggggcaaatg ttgtccatac aaaaagaagc
     8401 aatcgaaatg ggcgtcaata aacaagtatt tgcacaaatt cagattcctg cacttggttt
     8461 agcattgcca atttttaaag gggcaaatca atacactttg agtcttggag cggctactta
     8521 tttttatgaa gatgccgaaa tgggtaaagg aaactatgtc ctagcaggtc ataacatgga
     8581 aatgccaggt gttttatttt ctgatattca aaagttgtca ttaggggagg tgatggactt
     8641 agttagtaac gatggcgtct atcgttacaa ggtaacgcgt aaatttatcg tacctgagta
     8701 ctttaaatta atcgatggcg ttcctgaaga gaactctttc ttgtctttac caaagaaagg
     8761 agagaaaccc ttattaacgt tgtttacttg tgtttatacg tcacaaggaa aagaaagata
     8821 cgtggtacag ggagagctac aataacaaga atttaatcaa cgcgagtcgt tgaaatatga
     8881 aatgaggaga gtaaaaaatg gcaataaatc ggtatgtaaa aaaagctgga atgattgttg
     8941 gaatgttagg aatcattgca ggctgctttc aggggtatcg agcttatgca gaagatgtca
     9001 cgcaaaaaac gccaccagaa agggtcaata tcacggttca taaattaatg tacgatcaaa
     9061 gtacgcaatt gaacgttgat attgacggaa tcaaaaatga tggttatacg cacgatcaat
     9121 atcctacagg tgtaacgaaa tacaacaagg cagattatgg tgatgttgag tttacgttag
     9181 gaaatattac ggatcaagta ttaccgactg aagattctga tctaaccaac gcgaaagtgg
     9241 acgaaatcgt taaagatgta gaagacaagg gatctaacag tgagtacgtt aagaatgcaa
     9301 ccaataaaat tacttcagct gtagatgaaa atggtgagat cacttttgct gatcaaccag
     9361 cctatgtcaa ttccaaaggg aatgtatatg tggtttatga aagtaaatcc gctgctggat
     9421 tagtcacaca aaaagcaaaa ccaatggttg tgattgcgcc aatgacagac aacacaggat
     9481 caggattttt aaaagatatt catttgtatc caaagaacat tgtttctaag ttgagttttg
     9541 aattaacgaa atttggtgac gatggcacag ctcaaagcaa acaaacacct ttaaaagggg
     9601 caaaatttga gttatacaag ggcgaacctg gaaaaggaac aaaattagga gagcttgtat
     9661 cggatgatca aggaaagctg actgcaactg atttgacgtt aggaaaatac tactttgtgg
     9721 aagttccttc agaagttgta gttggatcag atcaagaacc aacagcagac caatatttgt
     9781 taggcgcaga cgctcgcaac gatgcgcaca ataagttaac ctttgaaatt acgaatgacg
     9841 gcgtgacaag tgatttaaaa gcttcctacg tgaactataa agcaccagtg atcgataaaa
     9901 cagtgacaaa tggaacagga gaagaacatt ctttccaaat tggtgacgca gtgaattatc
     9961 aaggtacaat tcatatccct acagatatcg caggtggaac agatgggatt acagtaaatg
    10021 gtgtaaaaag tgaaacttct ccttacagtg tatttaaatg gggcgacaca gcaggtaaag
    10081 gactttctta tgttgctgaa aaagcagata ttaaagtaac aaataatgat ggttcagtcg
    10141 tattaaaaga aaatactgac tataaaatcg aaaattctga tagtggtttt gtgatcgatt
    10201 ttatcgtaaa taatggacaa gtaagtgata cagttgcgaa tttacatggt caagatctaa
    10261 aaatgactta taacatgttt gtgaatgata gtgctgaaat cgcaaatcca ttaacaaata
    10321 gcgtggattt cgtttataac aataatccat ttaatcagga agaacaccac gaaaaaacga
    10381 aagctgacgt tgtgacttat ggcgcaaaat tcttaaaagt cgatagcggc ctatttggaa
    10441 caggtatcaa agcaacaccg cttgaaggcg cagaatttgc agctaagaat gcagaaggta
    10501 agtactacgg tggacttact gatacagata aagatggtgt caaagaagca gtttgggtag
    10561 acgatgtagc aaatgcagca atcctaaaat cagataaaga gggtcatttt gaaatcactg
    10621 gtttaactga aggagattac tcactggaag aaacaaaagc tcctgaaaac tatcaaaaat
    10681 taacaaaaga gatctctttc aaagtagaca aagattcata caaagaagaa aaccgaatca
    10741 cgatcaaaaa taatcaaaaa gcttccgtac caatgactgg tagcaacggt ttccaatctt
    10801 acgtattgat ctcttgcttg ttgcttgggg caggcgcaat ttctgcagtt gtttacttta
    10861 agaagaaagc ctagaaggat cagctaatga ttatcagaca ccctaagaaa aagcgaatca
    10921 tgggtaaatg gatcattgct ttctggttac tttcagctgt tggtgtctta cttttgatgc
    10981 ctgctgaagc gagcgtggcg aaataccaac aaaatcagca gattgccgca atcgatcgaa
    11041 caggaacggc agcagaaacg gattcttctt tggatgttgc taagattgag ttaggcgatc
    11101 ctgttggtat tcttacaatt ccaagtattt ctttaaaact accaatttat gacggaactt
    11161 ccgacaaaat tttagagaat ggtgtaggga ttacggaagg aactggcgat attacaggag
    11221 gaaacggtaa aaatccttta atcgcagggc acagtggact ttataaagat aatcttttcg
    11281 atgatttgcc ttcagttaaa aagggagaaa agttttatat caaagttgat ggtgagcagc
    11341 acgcttatca aatcgatcgg attgaggaag tgcaaaaaga cgaactacaa agaaactttg
    11401 tcacttactt agaaccaaat cccaatgaag atcgagtaac gctaatgaca tgtacgccta
    11461 aggggatcaa cacacaccgt ttcctagtgt atgggaagcg agtaaccttt acaaaatcag
    11521 aattaaaaga tgaagaaaat aagaaacaaa aattatcttg gaaatggctt ttaggaagta
    11581 ctgtttttct ttcagtcatg atcattggtt cgctatttgt ttataaaaag aaaaaataaa
    11641 ggagggtaac caacatgaga aatagaaaat ggctatttgt ttgtgcagcc gctctttttt
    11701 ctttcactgc ctacacacag ttgacgtttg cagacagaca agaaagcacg gatagcacag
    11761 aacaaacaat ccaaacgtca gatagcagca cgattcaaag cagcgatgaa accgtcgatt
    11821 cgtcaacagt tgattcttca gaagaaccta aaattgctga agacgatcaa atagtcaaac
    11881 agaccattca cgttcaaaaa attatttctt ccgaacaatt agacaaccag gggcaaaagc
    11941 ttgaaaaaca agaaggagta aatggcgcga agttttctgt ttatgatgta tcggacattt
    12001 tacaaaagat ggatgttaag gatctcacta ctgaccaaat tgaatcccaa ttaaaagatc
    12061 gagtgaaaaa actatccagt gatcaactga agctaatttc taatggagaa acaaagacaa
    12121 ttgatcaaca aacaggtgtc tttgaattct cggtagaagt acaggcaaat cagaaacaag
    12181 cttattatat tgtcaatgaa tcaagtcctg aaaacatttc taatagcgag gatattttgc
    12241 tattaacacc tgtttctgat aaaaatggtg aattcttaaa ggatgtgtgg atttatccta
    12301 aatcagaagc ttcgcaacct aaagaagaag taaaaaaaat cgtatctact ggcgtaaaga
    12361 aaaacttctt tgagaattgt tgggattttg ttactcactt gttttctaga gattaaggaa
    12421 gaaataaaat gaaactgatc aaattattat tattcaattt ttcttgttta accgcagtct
    12481 ttttgtactg cggtttatta ttttctaaac tatctatttt gccaagctac caactatacc
    12541 taatgaggat tttaaacata ccagctgctt ccatgccgct agatattatg gaaaagctca
    12601 ttaggaatcc attatttact acgatcaatt tgatcgttat tgtcggaatt tcactagtca
    12661 tttataaaaa agaggggatg aagggatgaa ttttaaacac tcgtggacaa gacggttaaa
    12721 atatgttttt cccataatga tgatgttagg tacattgttt gggttaaaaa cagccaatgt
    12781 ctttgctgaa aaagttattg tcgatccgtc aaatccaatc cagaacgtaa agaataattt
    12841 tattattcca aaagatgttc ctaatgcaaa aacaaatatt actgtaaacg gagcgtcaaa
    12901 agtttacaaa tatcatgttg atgccaacaa aggtggtttt acagacgact acgtaggatt
    12961 agtggaggga aacaaaaata tacgctatcc ttctttccaa aaagaatacg gtgaaacaaa
    13021 ccttgtttta gaaggcgtaa caaccaacac caaagtcaat attgactatg gaaaaatcgg
    13081 tacgtataac gggaaaaaag tcaatatcaa gctagttcta tctaatattc acttatactc
    13141 ggatacgttg ccgtggaatc tactagataa caactataca aaaactcatt ttcgagacga
    13201 tggctataaa aatacgaatg gtgcaatgaa taaatctaaa aaaagaacag tactttggat
    13261 ctcagacaat cttttctcag gaattgttta ccattcaaca caaatgaatg tgcagctagt
    13321 agccacatat gaggacggta gccccgttca attttcagga gatacattta tcagttttaa
    13381 ctctttgaac cctgcaggag gaaaaagcac ggatcttaaa ggagaatatg cccactatga
    13441 caaaatgaat acgacagatt ggtatgtcag aaaagatacg gttctttcag aatttaaatc
    13501 tttctacaac aatctgaacg tggtaggtgg ccacccagga ggaagctcta agctgacaca
    13561 agcggataat gatttcaata atttgcatga taaattagga gatccgaagt ttggtcaagg
    13621 tactgtgtcc tttaaaatat ctgaagcaaa tccgacgttt gtgattggtt cttctaatgt
    13681 tcaaacctgg tttactttat cttctgcgac aatctttagt gttgttcctg atcaacctga
    13741 aaaaacaggg gttgataaaa atggaaataa cgtgaacgat aaaatgttgc aagtaggaga
    13801 cacgattcaa tatcgaatca aacagaaggt gaatcgatta ggagtggatt tactagcggt
    13861 ttacgatcga tttgagttaa tcgataattt accaaaagaa gtaaattacg tcgatgcaca
    13921 cgttgaaagt ggtacaaata aaaaattcga tgtatctggt gaagtgacct atgacaaaac
    13981 aaaacaccag gtaaaatatg cagcgaaagc tgatacatta aaaaatcgaa tgaagtataa
    14041 cggcgaaact tacgaattag tgatcaatgt aaaagtcaat gaattggcca atcaaaatag
    14101 tgtagccaaa aatcagggaa cgtcgatcat taataaagta gaaaaggata caaatatcat
    14161 taccgtttat ttccctaaaa ttccagtgaa agaagtacaa caaaatggaa aagatgtaaa
    14221 cggacgaaat gacggtaaga agggtacgcc gacagctcct ttaaacgcag gatctgaagt
    14281 acaatatcta gtcacacaaa aatggcacac aaaaggcgta gacgctgcgt cagatcacta
    14341 caaacaattt agtattcaag atccaattga agctcgtttg acatataaag aaggttctgc
    14401 gcaagtaata gataagagta aaggaaaaga tattacttct gaaggcacac taacctatga
    14461 tagcaattca agaacgctga agtgggaagc ttccgctgac ttcttaaata acaatctttt
    14521 agatggacga gaaattcagc tgatatttac agctaaaact ccattacaat cagaaaaaaa
    14581 tattgataac caagccgtag tagcagtaga taatgtttct aataaaacaa atgttgtgac
    14641 aattggggtt gatcctaact tgccacaagt tattgttcct aaaacaggtt ctacacattt
    14701 agtaacgatt ttagcggtca gcttactatt acttgtgtta gcaacttttg gctacgcagt
    14761 tctgaagttc aattaaacaa aaaaacaagg ctgaaaatca gccttgtttt tctatttgaa
    14821 aatgggattg gcactaataa atacgttgtc tgaagaaagt gatccgtctg cagaaacttc
    14881 ataagcatgg gataaaccaa cgatactttg attgttgtaa agcaagtttg cgttatgagc
    14941 agcgctatct ttccacataa ccatagcatt tactgctcct gtatctgaaa ggatctcgtc
    15001 aacgtaaagg tagaaactat tgatattttc tcctttagca ccgtagcagc ctaagctttc
    15061 taagagatct tggaaagtac cgccgccagg tcggtaatga ctgaagctta cttgtaattc
    15121 ttttgcacga atatctgcag cttgttgaac gatatcattg aattgcattg gggcaacgcc
    15181 attttgttga cggtagctat ttaaccaagc aagttctttt tgacgcacat agttgtttaa
    15241 atcacttagt gagatatttt tgtatttgaa agtgatcgtt cgattttggt caacattatt
    15301 gattacacgt tgtccatttt tatcaacggt aacagatgaa tcgtcatttc tacacaagaa
    15361 gtagttttga tcggtcatat ttttccaaga agtagagaaa ctatctccct cgtaaaccgt
    15421 ttgtgtttca gtagataaaa cagcgtttgt gtcaacgttt atatggttca cagtgacgtt
    15481 gtattgatga gtataggcaa aattgattcc ttgattactt gtaacgttgt ttaaagtcac
    15541 agtgttgcta gagctttctc taggatcaag aacaaaatta gtgaacatag gtgcttcaat
    15601 tgtgatgctt tgtccataaa cgacgggata actcttactg taattataga gttcttggat
    15661 atttggatca gtatccgaga ctttatttgg acgagctttc acttcaacta gaaagacttc
    15721 atcatagtaa aaagtaaatg tggtatttgt gttaatcgtt tgtgtttgtg tttcattccc
    15781 atttacttga tactgtggtg cttcaaagtt agtcaagtcg gttctagctt ttgcagtgta
    15841 acttgtacca gcttttactg agacatgttc ttcttttagc agcgttccag tttgtcgatt
    15901 catatgacgg acagttaccg catattctga aggtttaggt ttgtgttgta accctttaaa
    15961 tgcagaatca agactatttt tagcattatt tacttgggtt tgagaagctt tagaatcgtc
    16021 tagtactttt ttagcattgg ataaagcagt ctgaaaatta ttccaagatc cttctgtaaa
    16081 atctccttta gcagttgatt ttacttggtt gtacaaggct tgtaattccg ttttgttcac
    16141 agtaggagct ggttttttct ttaatccctt gtaagcagaa tctaatgtgt ttttggcact
    16201 atccacttgt ttttgagtcg cttttgagtc atttaacact tttttagcat tgtttaaagc
    16261 ggtttggaat gcgttccagg tattgtcggt atagtcacct tttttagttc ccttaacgct
    16321 cgtataaaga gcttctaaag ctgatttatt cactttgggt ttagaggtgt cagcaccata
    16381 ccaaccaata ccttcgtatt tccagccata gtctttggtt aaccattgta cttctttcca
    16441 acttgcagtg tagtgatgcg aaccagaggt aacacctgga aggaaaaggc ggtagatcgg
    16501 tttattattt ttttcggcag aacgccaggc aggcccttca taagtccaac cgtaattttt
    16561 ggtcaaccat ttcacttcat cccaactagc tgtgtaatgg tgatcacgaa ggcctttatt
    16621 gtataaacga tagacaaggt ggccaccgct agtcggtgct tcccaagcgg ggccttcata
    16681 attaccccac ttaattttca ctaggtgatc acgttctttt gtgctagagg tgtaaaaatg
    16741 ttcatggtta tttggattat aaaggcgata catgacgtta gttgaagcag cgtcagcaga
    16801 tccagcaaga gcaactgtgg cagcaaatgt tccaatagct ataacagttt ttgggtttaa
    16861 taatttcatg ttttcaactc cctattaaat gattaaatat tttgtttaca ttcataatat
    16921 accacggtat accgaggtag tcaacggtat accgtggaaa ttttgaaata aaaaagccgc
    16981 cctatgaaga gtgtaaggat ggctgtttat atgctatgaa gaaatggatt cggcgtattt
    17041 ggttagtttg gcagcattat caaatcgcat tttttcaatc gcactttttt tctttattaa
    17101 atccgcaatt gttgattctg cgactgaaga atgtttgcta atttcatatg ctgtcttgcc
    17161 agtttcaaat agtaaccatt cgattttctc tttatcagcg tacattgcaa tgctccctct
    17221 tgattttttc tttattatag ctcttttttg taataaatca caatatcaag aggaaaagtt
    17281 tctatagaaa ggtgggaaat tatgagtatc cgttccccgt gtatcaaaat gaggtttgtt
    17341 gctactttcg ttatttaata tactaaaata aaacaacgaa agaaggaata gaatgaaaaa
    17401 gttaataatg gtatttactt cgttgattgc catgattgtt attttaggtg gatgtggtag
    17461 tgaaaaaaat aagcaagata gtcaagataa tggcgtgtta aaaccaggaa cagtgtggaa
    17521 agaagaagta ggcggacttg ttcataattt aaaaattata gatgatacaa catgggaata
    17581 ttccgaaagt gtttggcatc ctgaacaagt acaaataaca gttgagaagc taaaagacta
    17641 taaaggatta gaaagatata aggtaatcga ttcaggtgga gtagatttat tcataggtaa
    17701 aacagcctta atagttccct ataacaaaga cggtttagaa acgtttaaat ttattccaac
    17761 aaacgaaaaa gaacatcgag ataaaagtga aatattaaaa gaatacagta ctaaatctgg
    17821 atacaagtta caaaagacta gcgagtgatt gctagtcttt ttatttatcc tgaaggagga
    17881 atcgcagtgg caaaagctaa aacgccattt gaaaaagccg cgtctaaaat cttagaacgt
    17941 cgcggtgtat ctgaaacaca gtggaaacaa gaagtccttg aaaaagcaca atttgaccta
    18001 ttacggggaa aagacaagga cttagaagga tatatctatc aacgtgaacg tgaaaagctt
    18061 gttttcgatg aaattattaa acactaaaag gagcgaacaa ttttatgttt gaatcaatta
    18121 caatgaattt tttaacacaa ccgaaagtag tagctgcggt tgatattttt agagcattac
    18181 aaggtgcagc aactagttta aaaaacaatc tgatgatttt tgcggctgta gctgccgttg
    18241 cctccattgt tcttgggggg attttcttct tctttggccg aagaggagct gaaaacggga
    18301 agatgcacgt aggttatgta ttgatggggg taggagttat attagctgcc gcctctattg
    18361 tggtttcgtt atttggaatg tttggacaaa ccccacaggg actataaaaa ataaatagaa
    18421 ggagtaaaaa gtacgtggct actttttact ccttttttat gaaaggagaa tactcatgta
    18481 ctttacgatt tcaggcaata ccaaaaaaga taaagagtta ataaaaaaag caacgaagaa
    18541 agtgggaaag catactgcct ttttaccgaa aacaattaaa tattctgtca ttgaaacgga
    18601 agaacagcta catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt
    18661 gactctcacg ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt
    18721 tggcccactg gattatcaaa tttcagaagg tttttatcct acattgatgg atttgatccg
    18781 ccttgatgtg tttaaggaaa cggatgaaac catgagtgtg gaagagtctt atcgaaacga
    18841 aaaactgcta caagttttag aagaagagtt agagagcgac caaagaaaag tgagtcaagt
    18901 gagtgaagtc gaagtaaatg atgaagtacc atgggaacga ccaatctttc caaagaagga
    18961 gtcaaaagaa aaagagacag tctatgttga cgaacaagct gatattccga ctcagaaatc
    19021 gatatttgag gaagagaacg atgtaatggg tcaattggag gtagatccct ttgaagaaac
    19081 aatggaggat gaagagacgg ccgaagtaga agttgatcta gttgaagcta atgaagagcc
    19141 aacatggact gaaggggatt ctcaggaata tattgaacca cttatcagtg aagaagtaga
    19201 gaaaaacgaa acagagatac aaaaggcaac tgtatttgat tatattttgg atcaatatcc
    19261 gccagagcat gaatggatga aagaaaaaaa taaattattt ttaaatgagc tttattcgaa
    19321 atatcaatta ccccaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca
    19381 gggtaaaggc acacttttag aacagttaga gtctgttcaa tcaacaaatt ggcatgagct
    19441 tgcacaagaa gatttattag ccacttttga agaacgtcaa caagaaacag atcaagaaat
    19501 aaatgagtat gcgacggaac agtcatctaa ttgggagaga acaaaagagg aactaaataa
    19561 agaagaacaa cgagtgattg aagaagaagt acaaaaaatt aaagagcgat acgaaaagaa
    19621 acgcgacaat gcgtattcct tgtgtataga gaaaattaat aactttacta aaactaatca
    19681 agaagcttta tcgaaagaaa aagaaaactt gttaaaagaa cgtataaaag aacgaaaaga
    19741 agagttctac cgaggtttac gtacagataa gctaaagatt agcaaagaat taaatgaaca
    19801 actgaataaa ctttatcaaa caacgactga agttcttatg gaaagacacg ggcagatcca
    19861 gcaagaatta caagcacaaa ttccaaaatg gaaaaaagag tatgaaattc aacaacaaga
    19921 attgaaagaa caagaagaaa aacaacgaca agaagaaaaa gagcgcgaac aattagaact
    19981 aaaaaaacgt gagcagcatc ttgcagaaca aaaagtggcg atagaaaaac aacggttaga
    20041 gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt caagaacaaa tggatacatt
    20101 gcgacaagca cagttgaatt atctagcaca acctcaacca gtagctgctg ttcaaacgcc
    20161 agtgaacaac caagcaaatt taactccttc accacagcgt tcctttaacg gatggatggt
    20221 gggatgtatt gcaagtttaa gcttgcttct aggtggagga acagtatttg cttttaatca
    20281 tatcaatcaa tcttcacagg cagattctgt agccacaacg gcgtcaatca aggaagaagc
    20341 ccaggaacaa gcaagaaaag agtatgaaca acaattggaa gaattattga atcgtaccaa
    20401 tcagtcaaca gcagagtcaa gtatggaaaa tagcaaagag agtgatccta gtaaggctaa
    20461 agaaggatca agcgattcaa ctgtacactc aaacacttcc gaaagttcag aatcaaatga
    20521 ggtgaaataa tgcgtattag agaaacaacg agacgaaaag acaaacgatt caatttagaa
    20581 aagaataggt atctttctca tttctatcgc tatactcctt caggagaaga gctaccagaa
    20641 aaacatgaca aacgtatttc tcaaaagatt ttttatagtg gcttagctta tcttccttta
    20701 aactatgttg ttacaagaat accagaagca tttcatcgat ttgagattat tccaggaagt
    20761 ttacaaggag tgaatatttt tcagttacaa gaaatgccgc agttattcca ttttgattca
    20821 actggttttt ggaatccttt gaccccaaca acaagtttag gcacacgttt agttgtttct
    20881 acgatttgtg ttgttccagc tgtacttatg ggattgaaaa tggacatgtg gtttcgtccg
    20941 ctagcagatg ggcaaaaagg agataaccga ttaaaaacgt taaaggaaat ccaacaaatt
    21001 tatccgcaaa taccagaatc aaagggaagt tttcctggtt atggcggaat accaattact
    21061 cattataaaa aatactggta tattcagacg gatacagtaa atacttgtat tgttggtact
    21121 tcccgctcag ggaaaggcca gatagaagtt ttagcaacca ttgacaattt gtcgcgtgca
    21181 gaaagacaaa gttcaatggt tgttaatgat ccaaaaacag aattatatgt tgctagtaaa
    21241 gatgaactag aagctagagg gtatgatgtg tatgccttta atatcaccga tccactgcaa
    21301 tcaatgagtg ataatccatt ggcactcatt gtgaaatact ggaagcgagg agacatagac
    21361 accgcaacac agttaaccaa tacttttacg aatacgattt attatgatgc aaatgcttca
    21421 gacaataaat actttaacga aaatgcgcag aaagctgtca atgcgcttat ttttgcgtta
    21481 ttggaacaag cggatcaaac aggggattac tccaaattaa caccaaataa tctcgttgaa
    21541 ctactttcag aaattggcgg ttttaattat tatccagatc ctgagaatga attcaagcag
    21601 ctcaatgcct tagatgagtt catgaatcag ttaccccctg gaaatatagc aaaaaaacaa
    21661 tatggggcaa caaaaattgg atctgataaa gcgaaaggga atatcttgtc tacggcaatt
    21721 acaggattga atccatttac cctaacgaaa attgcgaaga tgacttctca atcgaccatt
    21781 gattacaagt ccattggttt tccgaagtat ttggatctca aattacatga aagcctatta
    21841 aatcaacgca ttgtcgtgga attttatcat aagggaaaga aatttcacga ggaacgagtc
    21901 aaagttggct atcgtggttt ttgtgaaatc aactttgatt gtcatttaga gcagggagat
    21961 catgtttgct taaaatacaa tcatcaagga aaacaatatg ttgcttggta tgagttttct
    22021 caaaaagtat tgaaagacga agagggaaaa atcgtttatc gaaaaaaacg aggagaaact
    22081 catttgccag aactagaaaa agaagccgtt ctaacattag atgagaagaa aagtaaccta
    22141 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgttaacg
    22201 cctgattatg atccatctaa ccatatcatt gttagcatct ttcttagtca gttgtataaa
    22261 gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgtag gattcatttt
    22321 attttagatg aatttggaaa tatgccaccg atggacaaca tggaaggaat catgacggtt
    22381 acggcaggtc gtaatatgtt atgggatctg tttattcaat cttatcagca actttatgcg
    22441 aagtatggag aacaaaacgc agcgattatc aaagaaaatt gtcagacaca tatttatatt
    22501 atgtcgacag atgataatac catcgaagaa ttttctaaaa aagtggggaa taagacggtt
    22561 gaacaagaga acagtaccat caattcatta ggtatgaata ctcatatcaa tcgcaatgtt
    22621 gattcagatc gtatcttaac tcctgaaaga atttctactt ttttagaagg tgaaacgatt
    22681 gtactcgctc ctttaaaacg acaagatttg aaaggtcata aaatacggcc gtatcctatt
    22741 tttaatcatg gggaaacgaa tcttccttat gcttatcaat atattgaaga tttcaatcca
    22801 tcgtgggata tcaatgatat agacatacat tgtacacatg cggacttgga tcttaaggct
    22861 ttacaaattg attatgatta tttcctacca gatgaatatg ctagaaatct ctatcatgga
    22921 ctgcatgatc aattagaaga aaaaaacgaa gaaaatatgt tttataaaat cgttcaatct
    22981 tttgagaatg agacggctaa aaaattgtta attcaatttt acgaagaaca agcaacggat
    23041 cgtcttcaag aatatgttga aaagttgatc aaaaatgaag aaatttctga aatgattagg
    23101 aaacggctgt tattggaagc tgaaaaggcg gtgaaagaat gatcattgga ggaattttct
    23161 ctggcatagc cgactggtgg tcaggtttat tcggagaagg ggatgcagat cccaaaaccg
    23221 taacgaaagt cttacaagat tatggtaatt atctgcagta tcaagatgta ttcagttgga
    23281 tttgtaatac ttttttatgg ggattgatca aagggttata taatctcaat actttattag
    23341 aaaaaacaat ttatcaaagc tttgatttaa aagacttatt gaacgccgct ggtgttaatg
    23401 aactatttca aagtttgatt agtaaggtat tggctctttt ttgtgtcgtc acattaattt
    23461 atttaggctt gaaatttttt atgagtaaac acccaccgaa agtcaaaaat atcttggtta
    23521 atttatttat ggccatgatc ttgatttttg gtggtagttc tttaatcgat gaagggttaa
    23581 atatcagtaa aaacttttat ggagacgtca ctgcagcaaa taaagcagat aaaacgggtt
    23641 cacctgcttt tcaaattatt caaaataatg tgattgatgt cagtacatta ctagcgactg
    23701 acccgaataa agtagaatcc ttaccaacag ataaaagaaa tgctttaaca gcgaaaaatt
    23761 tcaaaacagc gaatatcaat gggattatta cacctgatca agctaagaaa atgtctgaat
    23821 caagtgatat tagtacagta caaaaagatc ggttaaaggc attacagtat cgactagagt
    23881 tagacgataa tgggaaagaa atccctgttc aaattagtga tgaaggttta gcaaaatatg
    23941 tgtatacgtc aggctatcgt cgatacatta gtagtccagg gattattttg gctggggaat
    24001 cttctttgtc ggtagcctat ttatttattt tgtttacaat cgttacttgc attattgagc
    24061 tagtatttaa aaaattctat ttagtaattg ccgcgtcaac ggactttgag acaggacaac
    24121 ggatgaaaag tgcgatagaa gatatttctc aatccttctt gttattggcg tttacgattt
    24181 tagaattacg aatctataca ttaatgcttt caggtatcgg tgatttgcat gcggtaggaa
    24241 agatcaatgg attcttgtat gttgttgctt taattgtgct aactattgcg ctattcaaag
    24301 gctctcaatc ggttacgaag attttcggtg ttgatacgag tctgaaaaac ggcagcaatt
    24361 ctcttcttag catgtttgcc ttaggaaata ttgcgaaaaa tgtgggtagc ggagctaaaa
    24421 accttgcaag tgctggtaag agtggtttaa gccatttaaa tgatattcgt aagaatggat
    24481 ttggccatcg aaaagatatt acccatgata acggggaaga acaatcagac gataaagcta
    24541 caactgtacc taatagtaag aagacagttt ccgaaatgtt cgggaaagcg aaaaatggtg
    24601 tttctaaagc cgcagaaggt cttggttacg tcaatgaacg tgggttaaaa ggaacagttg
    24661 aagatcttac agaaaaagcc aaagacacct tagaagagaa attagacggc acgagagcta
    24721 aagaatttgc tgaagctgtg aagaatccat ccgaagcact agaaaaacaa aaagaaaaag
    24781 cttcagcagt caaagaagga ctaaaagatg cgtatggtga aggacaagtc aatgcagcag
    24841 tgaaggcaaa tacaacaagt gaaaaagaaa atcaagaagg tgcagcgatt ccttcagcca
    24901 aagatcacca agatgagtta cattctaacg aaaattcgat cggagaaaaa gattcagaag
    24961 aaggacaaca tggtcagtta ccaaaaaccg atccagaaca atatcgttat aaatctaaca
    25021 ttaattctga aaacgaaaaa cgcatttcag atttaccaaa acgaaatcaa gaagtacaaa
    25081 cggatgagga ttccatcgat ctgccaaatg ataatggcat cgaagttgga actgaaaaat
    25141 caacgaaaat tggtttgcct aagacacaaa atttaccaat gaaagatagt cgtttgaatc
    25201 aattcaaaga gaatgatcaa agctttgttc atcaatcacc tgcaggtact gttcaaatga
    25261 aatcaagtcg tttgcctgaa aatggaaatc tgacaactgt ttcttacaat ttagctgaaa
    25321 gtggtgaaac agagaatgcg tccgttcaaa atattgtgac aaaagaagct gcacctaagc
    25381 caatcaataa agttcataat ggatacagac cagcaaacgt agcatttact gcaccgacaa
    25441 aaataaatgt gacaagagaa actaaaaaca ggaacaatga attgaaagaa caactattga
    25501 aggatatcaa accataaaag gcctatttaa gggccttttt ctttataaaa caagtttaaa
    25561 atggaaagga gaaaattgtg gaggatattt taatagctac gtttataact gcactgggag
    25621 catttttaac gatatttgtg gtgactatta aaggatttaa agatacggat agtttacaaa
    25681 aagatcatca gtctatttta gatggtcaaa aaacacatac cgaaagcatt attgtacacc
    25741 tttcagaagg attcacttat ttgaaagatg ctatgcccac attcacagta aaattaacgg
    25801 aaatatttca ttctgttgag caaatatcgt cagaattgaa agagcaaaga caacaaaaag
    25861 atcgtcaact tggctcactt aattttaggc aagaaacgat acaagaatca ttggagaatg
    25921 taaatcgatt aaacgatgaa atgcaacgag ttcatttgga aaatgaagaa ttacgcgcac
    25981 aaaattatga tttaattcaa aagaaaagaa atctggaaca aaaaattgtt gaattaaaag
    26041 aagaaaatcg tcaattgaat tatcaattac gacgaggaaa aaatcgttct cgtgatttag
    26101 gaaatgaatt agagttgtaa tatttagaaa aacgctgtga gaaatcacag tgttttttat
    26161 ttctaaaaat acaaaatata aggggtatgg ttatattatg gataaataca ttcatatgaa
    26221 aaatcctcca agatttagtg gggaatggaa gtttttcgcc tttttctttt tagatctatt
    26281 aacgcttggt ggctgctttt tttgggcgaa aatagtcaat aatcttgtcc acttaccact
    26341 cttttttgca ctcttgaatt atggattatg gcttgcgata gcgttagtga ttgtttggcg
    26401 accctataat aaccctggtc aacgccaaat ttttgcgatt atcgaatcta ttttctttac
    26461 ggataagaac cactatcatg cgttagatcc taaccgaaat tatcatggta cgtataagga
    26521 ggatgaataa atgcgagtca aaggacaaaa ttcagttgat aagaaatcca ttttgcagtt
    26581 tggaaaacca gaagagcgct ctatcttgga cttatttcca tataaatcct gtacagaaga
    26641 gggagtctta attactagag aagatcattt tcagcgttat tatcgtgtcg tttccactga
    26701 cgtggaggga ttaaatgaac aagagaaact agaacgaatg aatcagttga ccaccgtcat
    26761 gcgtacgtat gtgccaacaa ttaagttagt tagtttgacc acagagacgg atctaacgga
    26821 acaaatcgtg caaaagcggc agttattaga taaaaatcgt atggaacagt cactaggaag
    26881 aaatctacag cgattaagaa aatatgaaaa gaaattagta gaggagatcc aggaattaaa
    26941 acgggcagaa caagaacgtc ctgacttaag cttctttttc gttattgaag gaaagacaat
    27001 caaggattta caaactagaa atcgtcagtt aatgcgatct agtggcattt tagggttaaa
    27061 accactctac aaaaaagaat tgattaagat tctttatcga atgaacaaca tgaatgacga
    27121 ataaaaaaga tggaggaaat gattcatgct agatgtgata aaaacagctt tttcaacgca
    27181 aagtaatcaa aaggtgattg atccattaaa acaacttggt aatcgtgaaa tagagatttt
    27241 aaaatcaaaa ggttatgatt tagatttctt aaaacaagtg atgccacaag gtgggttgaa
    27301 ttttgatgaa gaatatgtga cctatggaaa tggttattca cgatgtgtga cagtttatca
    27361 ttacacaaaa aatcctactc ttttttggtt ggccaatctc atgaacaatc ctcatacagt
    27421 ggccacacta gatgttagca cagatgaaaa gcaaaaagtg attgatcaaa tcaaccgcgc
    27481 attagatgaa ttaaatgatc gatcagataa tgaacgaaaa gcgactgacc gaaataaaag
    27541 tgcggaagaa tacctggaac tagcggaata tgcccgaaaa ttgacttcta atggggaaat
    27601 ttctaaacaa gtcaaagtga gagtacatgt ttacggaaat acgatccaag aagtggatga
    27661 acgggcgcag gaactgatcg aaaatctaaa agggatggat tatcgtgcgg tgacttatct
    27721 gcataaaacc aaacgagatt ggcaatcgat gtttaccatg ttcgatgaac aagctgaatg
    27781 gtttggaaat atcaaagggc aatcacttcc atcgttaaac attggcggag gattcccgtt
    27841 ccaccaccaa acgctaaaag atccaggagg cacacaatat ggggaaacca tgacaggcgg
    27901 cccatttgtc tggaatgcca cgtataaaaa tagtgtacgt ttatctacca ataccttgct
    27961 tctaggaatg atgggagctg gtaaatcgac cgttcttaaa atgatcgcgg aagcccattt
    28021 agctgcagga gactttattt ggggctttga aaagggaaaa gactttattc ctttcttaaa
    28081 ggaatataac ggtattatgg tgcgtttaga cggatctgac gggatgatca atcctttaga
    28141 gatctttgcc acacggacgt atgatgaagc atccagttta tatgacgatg gttcagtcaa
    28201 agatttgaag atcaatgaag cagcgtccta tcaaacgcac ttagacaaag tagtctatca
    28261 ggtacagtta gtgagtcctc agctaaaagg aacgatgaaa gcagaattca agacctactt
    28321 gaatcgtttt tatgaagagt atggaacagt accacgcggt tttacttcct caaattcgcg
    28381 ttcaaatact gatacgcaag tcacagggaa agatccagaa gcttatccga cgttcaaaga
    28441 atttttagcc tttttaggac aactggaact accaggggct tcccaagaga aaaagaatcg
    28501 gaaagaagaa atggagtcta tcgtggaatc cttatgtgag acttatggca tgatttttga
    28561 tggtcatagt acgattcgac accttgacca acaacagttg gtcttttttg acattgactc
    28621 tattacaggc ctagatcgag gggtacaaca atgtcagatg tacatggcca tgacgttgat
    28681 ttggaatcag gcattgattc aaggacgaag acaacgacgc ttaattaaag aaaaacaaat
    28741 ggaagaacaa gacttcaaac gcttcttggc gatgattgat gagtgccata atgcgattaa
    28801 tccagatttc attgaaacgg taaattatgt cacaaacttc caacgagaaa tgcgtaaagt
    28861 agaagcaatg actgttttag ctacacaatc cccacaagaa atggtgccag aaaatatgtc
    28921 ttctgataac ttctcgaaac tgaagaaagt ctttgaattt tcttctacga agatctttat
    28981 gcgtatggat gaatctattt tacgtcatat agaaggatta gttggtaaat caatgacgaa
    29041 ctcagaatta gaatccattc ctcaacaaaa tcaaggagac gctgtgatca attttggctc
    29101 aaaagaaacc attcgtgttc attttagtcc gaacaaacgt caattaaaat tgtttgatgg
    29161 aggaaaataa tggaaaacca aaatgaaagt ttaatcaaac agtatgttaa gcgtcgtgca
    29221 aagcggcgct tatttttgtg ggtgtttggt acttctgcag gtttgattac tatattgatc
    29281 actgtgtttg tgaccctttt tctaatactt gcggcaggtt ctattgataa ttctgattca
    29341 agtagtggtg gcgaagcttt tacaggagaa tattcggaag gcttacccat ttataaagaa
    29401 atcaaaggaa gaggcccttt ttctgatgag attgcgcaat atgctgtagg tgcagcagtg
    29461 aagtataaat tattaccaag tgtgatcctt tcgcaatacg gttatgaatc agcgtttggt
    29521 acgtcggcgt ccgcaagaaa tgatctcaat tattttggga ttacgtggtt tgatgggtgt
    29581 ctgtttccta aaggaactgc gcgtggaatt ggtggcattg aaggtggctg gtacatgaag
    29641 tttcccaact caaaagctgc cttctcctat tacggcttta tggtagccac acaatcaaat
    29701 tttaatgctt gtgtcggaaa taaaagccct ggcgcaagct tattgatctt aggacgagga
    29761 gggtatgccg ccgcaggaat tactgaagac agtgcctatt ataaaaattg tatgtctatt
    29821 attaatacaa ataaattgat ggagtacgat gagtttgcga ttaaacactg gagagaaggc
    29881 ggcggaactg gtggtacgat tacaggaagc tggggcaatc cattcccagg aagtagctta
    29941 gataaaaatt cgttcagtgg aggacaactt tttgggaaaa atccaggagg agaattccga
    30001 cctaacgggt tccatgatgg actagacttt ggttcagtcg atcatccagg aagtgagatt
    30061 catgcggttc atggcggaaa agtcgtgtac gttggcaatc ctggtatttc aggtttgggt
    30121 gcgtgtgtga tcgtgattaa ttacgatggc ttgaatatgg tgtatcaaga atttgcaaat
    30181 agtacaggaa attctcgagt gaaagtaggc gatcaagtga aagttggtca agtaattggg
    30241 atacgagata cagcacactt gcatttagga tttactcgaa tggattggcg tcaagcacaa
    30301 ggacatgcat tcattgatga tggtacatgg atcgatccgt taccattttt gaatagcagc
    30361 aaaaaataga tcaggagaaa aaatatggaa aataaaacct taaaaatcgt cttaacagct
    30421 gcaattagcg ttagtttaat cggcaatatt gcgttgtttt ggcagcttaa agaagaacaa
    30481 gagacaaata cctataaaat tgaagaagtg gctaaaaacg cgtctgataa gctcaaaaaa
    30541 aattcttctg aagcacaggc gaagattgac aagttaaatc aaaccatcaa tgagaaaaac
    30601 caagaaatcg atcagctgaa gcaacaagat ggcacaaaag caaaagagga agtaactgaa
    30661 gcacaaagaa agacggcaga agagttcggt caattggcca ttgataaatc attaagcaga
    30721 aatgaattaa cggaaaaact aaaagatgtc gcaacccaag aagtgatcga caaattaagt
    30781 cctgtagacg atgatcacca acatgatgat tacggaagtt actcttttac attaggtgac
    30841 gtgcaaactt acgctcaaac aagcagtgta aaagaggaac aaaattttgt cgtatttgtt
    30901 gattatacaa tcggcaatcc tcaattcaaa gatgtcaaac cacaaaaaat aaaaggtgga
    30961 ttaactgtca ctgaaaaaca agtagatggt caatggaaag tcacagactt tagttatttc
    31021 actcgttagg aggaagaaaa tgagtaagaa aaaaggagca agacaacgtt ttaaaaaacg
    31081 aaaaagttat cttagatggc tggggatctt tatgattctc ggctttttga ttcttgctag
    31141 tccgatttac acacataaaa aggtagacat accagatgaa gtaagcacga acgcattttt
    31201 aaacacgaag gaactgacga tggtttcaaa agaaaaagga gcgaaccagc aattagtttt
    31261 tgaatttgcg atcgacgatg aagatcagag cttattaaaa gaactagcta atcttaatta
    31321 tcgtgtagaa gtaaagacag ctaaagggga ttatcaagca ataaaagcca aagtgatcaa
    31381 ggtgtctgat gattattttg tagtgaagtt agcacatgta ccagaagaat atattgctat
    31441 gcgattaact attattcctg aaaaaattga tccaaaggtg gatatgcaag aaccgcaaga
    31501 tctgattttt tatattcacg aagataaggt caaagatcac gtgaaagacg aagattatga
    31561 acaacatgcg atcaattaca aagtgaaagg atacaagaaa gaacaaaaag cagcacaaaa
    31621 acaaattgaa tccttccaag caaccattga tttgaatgaa gaattagtga agaaactaaa
    31681 agatcaattg ccttatcaag tcgcagaaga tcaggaaaat actgaaaata agattaatag
    31741 ttaccagcaa gaaattgaaa catcgaaaac gcaaatgaaa gatcaagaag agagcattca
    31801 aaaacttcaa gaaaaaattg atcgtatgac aaaagaaaat atttagaaga ataggtggag
    31861 taaaagaaat gaagcgagcg aacttggtcg gcaaagatac tttcgctgag cagaaagtat
    31921 tgattaaagc ctatctcaaa gttgtaaaag ctaaagaaat atgtcaagaa gtgaattatc
    31981 taacacaaca atatctgagt tgaagaatgg tagaaagagt ttagatacct taaaattcag
    32041 atcatttgaa gctctatata tagctgctaa aaaatatttt acagctgata ataaataaaa
    32101 aattaaggtg ggttctatgt gaaaaaaacg tatcaaaagt ttttatggat tagctatatc
    32161 gtcctattta tcgttgggtt tctcgtcagt gtgtccgtga tcgtcggaac gctttttctt
    32221 ttgttctctg acgaagatct ggtacttaag gggttaatga gtacgttatc gtgtgtcatg
    32281 ttactttatt tatgtataca gatcaacgaa attatgcccc ctcattggca agaaaagaag
    32341 aaagaattta aggaagaaat cgaaataaaa tccaatgact ggattgaata cacatggctt
    32401 cctctgaaaa gaaatagcaa acgaaaggaa ttttgggcat tgcaattatg gccaatagga
    32461 aaacttttta tttatatggc ttcacttatg gttactttaa tcggttattt ggtggtggtc
    32521 tatggggtgg cctattactt gttaagtgag ccaaaacaga tcgcggcgtc aaatattttc
    32581 acggcagtaa tgacattaat gtgtgtcatg tgcttttcta tttttagtta cttactcgtc
    32641 caatttatca ggcaaaattt aacaatgagt aaacttttca aaagtttatt ggacttactt
    32701 atcttaggca gtatgttaac gactctaaca gatcggttcg aacaatctga aaaaacggta
    32761 gaggggataa agaacttctt aacgacgatt gatcaattta taaatggata tatgtatttg
    32821 tttgaagtag gctttattct cactttgttt ggttgtgtat ttataaaact cattaaaaga
    32881 tcaatcgaaa aagaaatggt tattgatcta attccgacgc tgagcggcta tgagtattac
    32941 gttgattttg gaaagtatga ggagttgatc catacggata tcgttcacac gagtatccat
    33001 gaagtaaccg ttaagggcat cagatctact ttaaaagatg gacaggagac gaaaacaatc
    33061 gaccgaagaa aagaggtcta caatttttat aaagttgaag caagtctacg atatggcatt
    33121 gggaaattga ggttagatgg gagtaatatt caacaaaaca aaccatatgt tttaggaaaa
    33181 gtggtgattt agaacttcct atgaaagatg aataaataag gagaatcttt tgaaaatgat
    33241 tcttctttac agaattgtgc ttatagttgg cacggagtta aaaatcttaa agaaggagca
    33301 gttattcaaa gtgaaagaac atcataatca gttactatgt attaaaacgg gtacgatgaa
    33361 cattcaatac cgaacagaat atttagaatt aacgaatcaa actgattttt tgtcgattcg
    33421 agagcaacat aagcaagaaa aattaatggc tacttatagc caattgattg atgagtggga
    33481 acaattcaag gaatctaaaa cataaaattc catttaaaca ccttattttt taaagagcat
    33541 aaggaaacgt gtacccactt tcaaaataaa gtgggtagat aggctgtgcc tatcacgttt
    33601 ctgaaaaaaa taaatgaccc caaaattttc aatttatgtg taggtttatg ggtagcaaaa
    33661 atatgaatgt gggcagcaaa aagttcattt gtgggtagta aaattcaaat gtgtgtagca
    33721 atgtgtgtag ctacacacat tgctacacac gctataacat atagtgaaaa ggagatattt
    33781 atggaagtaa aaataagaga tattgatgaa aaaacgattc aaagaattga ccgaagatgt
    33841 attgaattaa gtgaacgcac cgggactaaa tggagccgaa atcgatacct aaaagttttg
    33901 attgaaaatg attttgacca tgaacttatg gatcatagaa aagatcaatt tgatcggttg
    33961 ttagaaaaat ttgtggatat tcaaacttat aatacgaaaa ttttagaaga atatattgca
    34021 acaaataatc aattaatcgc cttgttaatt gaataataaa aatagtattg tgaatttata
    34081 aaaggaggaa atcaattttg gcaaaagcaa caagaaaaca tgtatatctt tacgatatgc
    34141 atatcgaagt acttgatgaa ataatttcta ctcatgaaga tatatctaat tatagtgaag
    34201 cagtcagaca tttgtgtatg atgtatgatc caaaaatcga agaaaaaata aatccaattc
    34261 aaattaaatt gaatgcaatg ggaaaagaaa tttctatatt aaccgaaatg gtttcagagt
    34321 ttgcggatct tcatttgaaa gatacaggaa tactaattgg tacaaattca actgtctatc
    34381 aagaagcaaa agagcgtgtg gaagggaaaa taaaagcgaa tcaaacgaaa aagtattctc
    34441 ctaagatcaa gaaaccaata aatgaatctg ttcatgccag agtagacccg tttgatccaa
    34501 caaagttttt aaaaagataa aaagtagaag gggaacatga tgaataaatc gcctgcagtc
    34561 attctcactt gtcaatttac cttgcccaat aaaaaatctt tttcaacata cattgattat
    34621 atgacaagag aaaaagcgct ggaagaaaag gaacgacgca cgccggacga agaacaggaa
    34681 ctgcaacgaa tcaagaatgc tttagaggat ttttatattc ctgaaggaga aactttttcg
    34741 gagagtaaga cagataaaga attcagtgaa acgtcgaatg aagctcaatc gttgatggaa
    34801 catggaatca attttgacaa gttagaggaa caagatttta ccaaatatct ttcttatatg
    34861 acccgtcact atgcgttaga acaaaagaaa gaactgacgc aaagggaaca aaaagaatac
    34921 caacggttga accaagcaat gaaaaaatat caaacagagc cacataaaaa agcttcagga
    34981 aatcttcctg gagttttttc tatggcttca gacgaagtga aaggaagtga tctgaaggag
    35041 atcaaacaaa tcttccaaag agggcaacaa aaaggctcaa tcatttatca agatgttgtc
    35101 tctcacgaca attcgtatct tgaaaaaatg ggcttgtacg atcccaaaac agatacctta
    35161 gatgaagaag gattaaaagc agctggaaga aaaatgatgg agacattatt cgagaaagaa
    35221 caattgaatg aaacaggtta ctggatggca acgattcacc gtaacactaa gcatatccat
    35281 attcattttg cagttgttga aaaagaaaat acgcgtaaac cttacacgga ggttgaaaat
    35341 ggtgtgagtt atatcgtacc gaaaggtaag agaagacaag caacattaga cgcaatgaag
    35401 accaattatg tacatgagtt agaacgtttt tcttatgaaa aggatcgaat gattggtatg
    35461 gagaaacgaa atttattgac gcggaaaagt gatttgcgta acaccttaac aaaagtggta
    35521 aaagagccag cgcgttacga tcgacacgca atgaacttat taaatgaagt atataaaacc
    35581 ctacctgaca aacgttcgga atggcattat ggggatgaaa aacgaaccaa actttcacca
    35641 tacacaagag agaaactcga tgagttaacc aaatatgtgt taacaaaaga acctgaatat
    35701 caagaatata tacagatttc aaaatcattg aaagaagaag caaaaagtct ttatggggaa
    35761 agcaagagag aatcaaagga tgcagaaaaa aatgctctct ttgatttgaa gaagcgcaca
    35821 ggaaatgcca ttctaacaga actgaagaaa caggatagtc aaataaaacc tttaatagaa
    35881 agtatggatc agatgcaatc tatttacaat gaacgatcct ttcgaacgct taaacagtta
    35941 ccaacgaatg aatacaaata tagttacgaa gaattcatga agcgcaggaa acaattccaa
    36001 caaagagttg tcacgcaacg gagtttgcgt caattaaatc gaacgattaa tgcccatgaa
    36061 gaaaaatacc acgcaagtaa agcctacgaa gagcaacaac gaagaattca aatggaacaa
    36121 gaaagacagt aaagacagta ggaggaatag caatgaaaca atatttagat caatggaaag
    36181 ttatagaagg atcattaaga gaagaacgaa tcgaacaact tcccgattgt ttagaaaaag
    36241 agcatttgtt tcagataaga gaaatgttga gaaacgaaca gtttgatcca aatcaatttt
    36301 tagtcgttga gtactcagct actggtgtgt actgttgtaa tcatgtgaag ggtgaaaaat
    36361 attttatcat tcaagaatat gagggcaagt tagcgcctta ttatactact tgggaaatga
    36421 atgaagaggg gatcaataac ttcccgtgta aaagcattga agagtctatt tcactcacag
    36481 aatgttagtt gttaagtgaa taaaagaagt aaaagagtcc tgaacgtaat tgtccaggac
    36541 tctttttttc aatgaattgt tttccgccaa ttcaatgagt ggtgcgattt gcgactacat
    36601 tatatcaaaa gaaagagaag ggaggaagaa gaaatggctt attcaacgat tggagttgtt
    36661 gttgaaaaaa gcagagacaa tctcgttttt gtgacagaaa ttcaaacggg cagagctttt
    36721 gtcgtcacag acaaagcggc aaaagcgtat caaagtggcg atattttaac gttaaatctg
    36781 actacgaaga cgttcgtcga tgcggcagaa gattatcc
//
LOCUS       DASHRQ010000023        36606 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.023, whole genome shotgun sequence.
ACCESSION   DASHRQ010000023 DASHRQ010000000
VERSION     DASHRQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.023"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            25..696
                     /locus_tag="U2M88_001267"
     CDS             25..696
                     /locus_tag="U2M88_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEM8373658.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="U2M88_001268"
     CDS             755..1195
                     /locus_tag="U2M88_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373659.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="U2M88_001269"
     CDS             1207..1875
                     /locus_tag="U2M88_001269"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEM8373660.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="U2M88_001270"
     CDS             1939..2562
                     /locus_tag="U2M88_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEM8373661.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="U2M88_001271"
     CDS             2618..3004
                     /locus_tag="U2M88_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEM8373662.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="U2M88_001272"
     CDS             3217..4608
                     /locus_tag="U2M88_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEM8373663.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5219..5587
                     /locus_tag="U2M88_001273"
     CDS             5219..5587
                     /locus_tag="U2M88_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEM8373664.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5767..6708
                     /locus_tag="U2M88_001274"
     CDS             5767..6708
                     /locus_tag="U2M88_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEM8373665.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6777..7508
                     /locus_tag="U2M88_001275"
     CDS             6777..7508
                     /locus_tag="U2M88_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEM8373666.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7652..9832
                     /locus_tag="U2M88_001276"
     CDS             7652..9832
                     /locus_tag="U2M88_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEM8373667.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9852..10334
                     /locus_tag="U2M88_001277"
     CDS             9852..10334
                     /locus_tag="U2M88_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEM8373668.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10350..11111
                     /locus_tag="U2M88_001278"
     CDS             10350..11111
                     /locus_tag="U2M88_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEM8373669.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11242..12576
                     /locus_tag="U2M88_001279"
     CDS             11242..12576
                     /locus_tag="U2M88_001279"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEM8373670.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12883..13368
                     /locus_tag="U2M88_001280"
     CDS             12883..13368
                     /locus_tag="U2M88_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEM8373671.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13361..14044
                     /locus_tag="U2M88_001281"
     CDS             13361..14044
                     /locus_tag="U2M88_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEM8373672.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14170..16815
                     /gene="polA"
                     /locus_tag="U2M88_001282"
     CDS             14170..16815
                     /gene="polA"
                     /locus_tag="U2M88_001282"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEM8373673.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16860..17696
                     /gene="mutM"
                     /locus_tag="U2M88_001283"
     CDS             16860..17696
                     /gene="mutM"
                     /locus_tag="U2M88_001283"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEM8373674.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17660..18289
                     /locus_tag="U2M88_001284"
     CDS             17660..18289
                     /locus_tag="U2M88_001284"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEM8373675.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            18341..18673
                     /locus_tag="U2M88_001285"
     CDS             18341..18673
                     /locus_tag="U2M88_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEM8373676.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18985..19476
                     /gene="nrdR"
                     /locus_tag="U2M88_001286"
     CDS             18985..19476
                     /gene="nrdR"
                     /locus_tag="U2M88_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEM8373677.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19499..20872
                     /locus_tag="U2M88_001287"
     CDS             19499..20872
                     /locus_tag="U2M88_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEM8373678.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20872..21798
                     /gene="dnaI"
                     /locus_tag="U2M88_001288"
     CDS             20872..21798
                     /gene="dnaI"
                     /locus_tag="U2M88_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEM8373679.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21927..22367
                     /locus_tag="U2M88_001289"
     CDS             21927..22367
                     /locus_tag="U2M88_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEM8373680.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22511..23212
                     /locus_tag="U2M88_001290"
     CDS             22511..23212
                     /locus_tag="U2M88_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEM8373681.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23209..24330
                     /locus_tag="U2M88_001291"
     CDS             23209..24330
                     /locus_tag="U2M88_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEM8373682.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24345..25577
                     /gene="pepT"
                     /locus_tag="U2M88_001292"
     CDS             24345..25577
                     /gene="pepT"
                     /locus_tag="U2M88_001292"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEM8373683.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25709..26617
                     /locus_tag="U2M88_001293"
     CDS             25709..26617
                     /locus_tag="U2M88_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEM8373684.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26652..26924
                     /locus_tag="U2M88_001294"
     CDS             26652..26924
                     /locus_tag="U2M88_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEM8373685.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26935..27981
                     /locus_tag="U2M88_001295"
     CDS             26935..27981
                     /locus_tag="U2M88_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEM8373686.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28232..30841
                     /gene="clpB"
                     /locus_tag="U2M88_001296"
     CDS             28232..30841
                     /gene="clpB"
                     /locus_tag="U2M88_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEM8373687.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30992..31678)
                     /locus_tag="U2M88_001297"
     CDS             complement(30992..31678)
                     /locus_tag="U2M88_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373688.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31656..32150)
                     /locus_tag="U2M88_001298"
     CDS             complement(31656..32150)
                     /locus_tag="U2M88_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEM8373689.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32170..33390)
                     /locus_tag="U2M88_001299"
     CDS             complement(32170..33390)
                     /locus_tag="U2M88_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8373690.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33537..35372
                     /gene="lepA"
                     /locus_tag="U2M88_001300"
     CDS             33537..35372
                     /gene="lepA"
                     /locus_tag="U2M88_001300"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEM8373691.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(35466..36593)
                     /locus_tag="U2M88_001301"
     CDS             complement(35466..36593)
                     /locus_tag="U2M88_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM8373692.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        12186 a         6473 c         7824 g        10123 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacaga tcgtttagga ggaaacagat
     5221 gtttacgaat gaaataaaaa tcatgcttta tgtggacgat gtagaaaaaa atgcaacgtt
     5281 ttggagagca gttggctttg cggaaaaaga tcgtcaagaa atggatggga cgcttataat
     5341 tgaagtagga gttgaggaca gtcaaacctc atttctgtta tatgatcgag catttgttga
     5401 gtcccattca cctggcacag ctactagtgc cccatctttg atgttttaca gtaaagatat
     5461 ctttggacta tataaaaaaa tgcagacgca atcagtcaca ttaggtgata tggtacagct
     5521 tggagaagaa tatgtcttta actttgctga tcccgatggc aactactttg cggttacagg
     5581 taaataatag aaataaagac tttgtttttc tgtgcgaaac aggaaagcga agtcttttta
     5641 attcaaataa taatattatg taaacaaaaa actttccatg cgtaattata cacaagcaaa
     5701 taaatcgtca aatgcgaacg ccttctttat actgtttagg aacgaaaaga aggaaggaag
     5761 aaatacatgg attatcaagt actgctttat tacaattata caaaaatcga agaaccagag
     5821 gaatttgcca aaaatcattt agccttttgc aaatctttgg atcttaaagg tcgtattcta
     5881 gtagctgaag aaggaataaa tggtactgtg tctggaaccg ttgaaaacac gactgcctat
     5941 atgaaagcaa tgaaagctga tcctcgattt gctgatacga tgttcaagat cgatccaagc
     6001 gaaggacatg cctttaagaa gatgtttgta agataccgtc cagagcttgt aagtttaaag
     6061 ctagaagagg acattgatcc aaaccaagtg acaggtgcct atctttcacc gcaagaattt
     6121 aaagaagcta ttttagatga acagacagtg gtgattgatg cacggaatga ttatgaatat
     6181 gacttgggac attttcgagg ggctgtacgc ccagatatac gcagttttcg agagcttcca
     6241 gactggatcc gcgagcacaa agaagaattt atggaaaaac gggtagtgac ctactgtact
     6301 ggtggcattc gttgcgagaa gttttctggt tggcttgtcc gtgaaggatt caaagatgtc
     6361 ggacagctgc atggcggtat tgctacttat ggtaaggatc ctgaagtcca aggagaatta
     6421 tgggacggca agatgtatgt gtttgatgaa cggatcgctg tcgatatcaa ccaagtagat
     6481 aaacaaatcg tcggtcgcga ctggtttgac ggaacaccat gtgaacgata tatcaattgt
     6541 agcaatcctg agtgcaatcg tcagattctt gcttccattg aaaatgaaga aaaacattta
     6601 ggagcatgta gcatagagtg tgcgaaacat ccgcgcaatc gctatgttca agaccatgga
     6661 atctctccag aagaagcaga actgcggttt acagctttaa ttaaatagaa aacaatagtt
     6721 ttgattgtcg accatttcca atatgcgcta tactagcgtt gaggatggtg atccacatga
     6781 aatcattgat ttatgctatt ggggatgtcc atggcaaaca tgagcttttt gaaaaactta
     6841 ttcactatta tgatccaaag atccatcagc ttgttttatt aggggatctc aatgatcgag
     6901 gtcctcgtac aaaagaatgt tttttattag gaaagcaatt agtcgaagaa acaggagcca
     6961 tttacctccg gggaaaccat gaagaatatt ttttgcaatt tctgcaatca ccggaggact
     7021 ggtatgcgcc ttatatgaga aatggtggaa aagaaacgtt tgaaagcctg attcacccag
     7081 gggcatcgga ggaatactcc ccaactgaaa tctcgatgat gatccaatcg agatattcag
     7141 aactgattaa atttttagtc cagcgcccct tgtattatga atgggaaaat tatctttttg
     7201 tccatgccgg tgtggatttg acaaagaaag actggcgtaa gacaagtcta agagacttta
     7261 tttggataag ggaaccattt catgaaggga aaaacaatac aggtaagaca attgtatttg
     7321 gacatacgat tacaccaatg ctccatggag atatgcagac aacagacctc tggattcagg
     7381 atcataaaat cggaatggac ggtggggctg tattcggagg gtcattgcat ggggttgttt
     7441 ttgacaagga aggtattctt caagatattg aatatcagaa caacgaagga ccttggcatg
     7501 gaatctgact aatggaaaga atgaaggagc tgtgactttg ttgcggctct ttttattttt
     7561 atagagaata ttgtttcttt cagtagttta acccaggata aaaagtatga tagaataaag
     7621 aagcaatttt tagcagaaaa gaggcatttt aatgacggaa aatttgaacc agacgatcat
     7681 tcagcttgta caaaaagaac tttcagacta tcgtcctaag cagttgacta ctgtacttaa
     7741 tttgttaaat gaaggaaaca ctgttccttt tatcgcgcgt taccgaaaag aaatgactgg
     7801 aagtcttgat gaagtccaga ttcgtgaaat cgaagagcgg tatgcctatc ttgaaaactt
     7861 agaaaaacgt aaaactgaag tgattcggct aatcgatgaa caagggaaac tgactcccga
     7921 gttagaagca gaaatcaccc aagccgtgaa aatgcaacaa gtggaagatt tgtatcgtcc
     7981 atacaaacaa aaacgacgga caaaagcaac gattgctaaa gaaaaaggac ttgaaccact
     8041 cgcgaagtgg ctgatgcagc ttacagatgg agaggtgcaa tctgaagcag aaaaatatat
     8101 tgacaaagaa aaagaagtct cttctgccga agaagcgttg cacggtgctc atgagattat
     8161 tgctgaacaa gtcagcgata atgcaaaatt tcggacttgg attcggtctt atacgtataa
     8221 caaaggtatg tatgtcagca atgtcaagga tgagcaagca gatgaaaaag gcgtatatga
     8281 aatgtattat gactttgcag aacccgtcca taaaatggtt tctcatcgaa tattagctac
     8341 taatcgtgga gaaaaagaag atattctgaa agtattcttt caagtagatg aagcagcaat
     8401 tttagcttac ttggatcgcc aaatagtcaa aaatcctgcc tctccttctt cttcattcgt
     8461 cagagaagcc tatcaggata gttataaacg ctttattcag ccagcaatcg agcgagaact
     8521 gcgcaatgaa ttgactgaaa aggcagatga gcaagcaatc gctattttcg gcgaaaacct
     8581 ccgtaatctt ttgttgcaac cgccactaaa aggtaaagtt gtcctgggat ttgatccggc
     8641 ttatcgtact ggatgtaaac tagcagtcgt agatgcgaca gggaaagttt tagcaatcga
     8701 agttatttat cctcataaac ctgcagcgca agcaaaaaga gaagctgctg gaccagcttt
     8761 tatccaatta attaataaat atcaagtaga tatggtagct atcggaaatg gtacagccag
     8821 tcgggaatct gaactttttg tggctgagca gttgaaatcc gccgaccata aaacctatta
     8881 cgctatcgtc aacgaagcag gcgcttctgt ttactccgct agcgaaattg cacgaaaaga
     8941 atttccgcat ctacaagttg aagaaagaag tgctgtttct attgcccgtc gcctgcaaga
     9001 tcctttagct gaattagtca aaatcgatcc taaagctgtc ggagtaggcc agtaccaaca
     9061 tgacgtttct caaaaacgtt tggctgaaca attggatttt gttgtggaaa cagcggttaa
     9121 ccaagtaggt gtggatgtga atacagcaag cccgcagctc ttgcagcata tatcaggatt
     9181 gaataaaaca actgcccaaa acattgtcat ttatcgtgaa gaaaacggag aattcaccgc
     9241 acgaactcaa ttgaaaaaag tgccacgctt aggaccaaaa gcatatgaac aagcaatcgg
     9301 attcttgcga gtccctggtg ggaaaaacat tttggacaac acaggtatcc atcccgaaag
     9361 ttattctatt gcaaaagaaa tattaacttc agtccaactt tcagaaaaag agcttggtac
     9421 agaagaagca gtagaaaaat tgacacgatt atccgtagag aaactggctg aaagcctatc
     9481 agtcggagaa gaaacgttag ccgatatttt agctgggctg acacaacctg gacgagatat
     9541 gcgtgatgag atgccagcgc ccttattacg tacagatgta ttgagtatgg aagacttgaa
     9601 accagggatg gaattgactg gaacggtccg taatgtcatt gacttcggcg catttgttga
     9661 tatcggtgtg aagcaagacg gacttgtcca tatatcgaaa ctcagcaaaa aattcgtcaa
     9721 gcatcctaca gatgtcgtgt cagtaggaga tatcgtaaca gtttggatag aacaagtcga
     9781 tacgaaaaaa ggtagaatca gtttgacgat gctttcccct tatgaagagt aacaatgagg
     9841 agtaacaaaa attgacacaa gaagaactgc agcagttagt agaagagata tcattaaaaa
     9901 gcttccagct tcctttcaag caccaagcaa tgttcaatcc acgcttacgt acaactggcg
     9961 ggcgctatca tttaaacgat catcatttag attttaatgc gaagctattt gaagagtcag
    10021 acgaagagac gattgcagga atcatcaaac atgaactttg ccattatcat cttcatctag
    10081 ctggaagagg gtatcagcat aaagatatgg atttcaagta tcttttgaaa aaaacaggag
    10141 ggttgcgcta tgcaccagcc ttgacgaaga aacagataaa gatggagtat tatcgatgcc
    10201 aagattgttc tgccacgatc tgccgcaaga gaagaatcga tgtacgcaaa tatcgttgtg
    10261 gaagatgtca tggagaaatc aaatggattg agacaaaaag agaagacttt aatgaagcac
    10321 aaaaaatcag atgaaaggat agaaggcctt tggaaaaaag tgagcagagg aatctaaaag
    10381 actggaatca gctattaaat tttttagccg cacctgaaga gggaaatttt gaggaactga
    10441 cggcacctac acttatttta gctggaaact gtcttcctat cttagctgat aaagcagcac
    10501 aaatgtattt aaatggccaa gtagatcaat tgtttttggt cggaggggtg gggcatgcga
    10561 ctagaatcct ttatgagaat tttgaaaagc aaggatttca ttttgaagat ggcatgagtg
    10621 aaagtgaaat ctgtcgtcag tatctaaaag aagtctatga tttgcccgat aaggcttttc
    10681 tgatagaatc gaagtctaca aattctggag agaacgccac tttttcactg gagattcttc
    10741 attccttagg agctgtacca gaaaaaatct tattgatgaa tgatccgaca ctgcaacgca
    10801 ggactcgagc aacttttgag aaagtatggc aaaatgaaca aactattttc gcgaatgctg
    10861 tgccatttgt gccggaaatc cttcactttt ctgaagagat catctttaca gcaaaagaac
    10921 tgaatcatca atggcctaag gaatatttcc atgcgctagt attaggagaa atggaacgtc
    10981 tccatgacga tgagaacggc tatggtccga aaggaaaaga ttttattcct cacattgata
    11041 tatcagagga agtttggtct tcttacacca gaattaaaga gtcaataaaa acggattttt
    11101 ctcgtacttg accattttag caagcgaatc ctcctttcca aataaatttg gttgaaattt
    11161 tcaatcgttt ataagataat gaggagcgtg ttgaatgtaa tagctaaaag aatggacgct
    11221 agataagaga agaggattca aatggaaaat caaaataaat tgtatcattt tgttggtatc
    11281 aaaggttcag gaatgagctc cttggctctt gttttacatg aacaaggtct aaatgtccaa
    11341 ggatcagaca ttgaaaaata tttctttaca caaagagatt tagaaaaagc aaatatcacg
    11401 attctgccat ttaacgcaga taatgtaaaa ccagggatga cgatcattgc aggaaatgca
    11461 tttcctgatt cacatgaaga aatccagcga gcaaaagaat taggtttgga agtgattcgc
    11521 tatcacgact ttattggtca ttttatccaa aattatacga gtatcgctgt aacaggctct
    11581 cacggaaaaa caagtacgac tggactgctc tctcatgtat tgagtggtgt ccgtccaaca
    11641 agttatctaa ttggagatgg aacaggacat ggtgatccgc aagcggaatt cttttcattt
    11701 gaggcctgtg aatatcgccg tcatttcctt gcctattcac cagactatgc aatcatgacc
    11761 aatatcgatt ttgatcatcc ggattattat acaagtatcg acgatgtatt tacagctttc
    11821 caaacaatgg ctggacaagt gaaaaaagct atttttgcgt atggtgacga tgcttattta
    11881 cgcaaattaa aagcaaatgt gccgatttat tattatggtg tcacagaaaa cgatgatatc
    11941 caagcacgaa acatcgaacg cacaacttct ggttctgctt ttgatgtcta tcatggggat
    12001 gaatttgttg ggcacttcac tgtaccagca ttcggtaaac acaatatctt aaatgcttta
    12061 ggtgttattg cagtagctta tttcgaaaaa ctggatctga aagaagttgc agaagaaatg
    12121 ctgactttcc caggagtaaa acgtcgattc agtgaaaaga tcgtagcaga catgaccgta
    12181 gtcgatgatt atgcgcatca ccctgcagag atcaaagcaa cgattgatgg cgcacgccaa
    12241 aaatatccag ataaagaaat aattgcagtt ttccaacccc atacgtttac gcgtacgatt
    12301 gcattgatgg atgaatttgc agaagcactt gatttagctg ataaagttta cttatgcgat
    12361 atttttggtt cggctcggga agaacaagga aacgttaaga ttgaagacct tggagcgaaa
    12421 atcaaaaaag gtggagaagt catcaaagaa aataatgtat ctccattgtt agattatcat
    12481 gatgcagtag taatcttcat gggtgctgga gatgttcaaa aatttgaaca agcgtatgaa
    12541 aaacttttaa gcagcacgac gaaaaatgta ctgtaataca attactgcag tgtccttata
    12601 aaaatagaaa gagggggagt ggcaaaaatc ttgccaagcc cccttttttg caacttatga
    12661 taaacggtgt tcaaaagctt gcatctgcgg taataagccc aatcaaatca aaaacatgag
    12721 aagctgtttt ttcttggctg ttcttattat ttgatgcagg acagcttttt cacgacctct
    12781 gtaaactata ggaatctgtt aaatcaaggg agcagaactt gccttttacg ctacttttgg
    12841 tacaataatg tatgattaaa aataaactgg agggtttcta tcttgaacat aggtaagccg
    12901 agaaaactag gaaaaacaat aaatgaaatc gaggaaggcg actcgttatc cttaacagaa
    12961 tctattgaag ataaccagct tttgctgtat ttgggattga cgaatgatgc aaatcctttg
    13021 tatatccaac atgactttgc acaaaaaaca gaatatcaac agccaatcgt tccatcagtg
    13081 atgctgatgg ggatcatcac aagtgcaatt tcaaagcatc ttcctggacc aggatcacat
    13141 gttgtcaatt tttctgtcaa ttttattgaa ccggttttcc attatgaaac actgactttt
    13201 cagtttgagg tcataaaagt agataagatg aaggatgtgg tgacgatttc tgtagaagcg
    13261 gagaatacga acgaagatcg tgtgttggat gctgtcgtta tggtccaacc gccacaagtt
    13321 cctgaattgg aaatagcaat cgaaagtgaa ggtgaattga atgaataatc caagtacatc
    13381 aatgtcaggt atcaatcgtt tttatgcaaa agtttatgga tttttaggta tgggacttgc
    13441 ccttagtgca gtcacttctt acttggctct ttgggtattt cctttgcaat tagcaacttt
    13501 tatcaataat tttccactag gctttgtggg tctgtggatc gttgaactga ttttagtaat
    13561 ggttctaggc gtaaaagcac aaaagaatcc gagcttagca atcggtggat ttattgccta
    13621 ctctcttttg aatggcataa cattagcggt tactcttgca tactacgata ttggaacagt
    13681 aacgcaagct ttcttgacag ctactttgat gttcatgggg atgtctgttg tcggagtaac
    13741 aacaaaaaga gatcttagcg gggttggaag agcaggacag gcagctttaa tcggagtgat
    13801 cattgcacta gtcttgaaca tcttcatttt gagaagtagc gtagttgagc tgttcatctc
    13861 cctactgtta gtcgtcatct ttgcagggat tacagcttat gataaccaaa gcatccgtaa
    13921 tatctatgtc cagacaaacg gaacagcaca tagtggaatt gctgtattca tggcattgaa
    13981 gctgtatttg gactttatca acttgttctt ggcgtttctg agaatctttg gacggaacaa
    14041 ctaaaaaaga aaaaaattca tcagttgggg ctataccgaa ttatttggta tggtcccgct
    14101 ttttcttttc gcattccgca ggattctttg ttagaatgaa taagaacttt acagtaagga
    14161 gttaagaaga tgacaaaaaa taaattatta ctcgttgatg ggaacagtgt ggcttttcgc
    14221 gcattctttg cgcttcacaa ttctcttgaa cgtttcaaaa atagaaacgg tcttcataca
    14281 aatgcaatct atgcattcaa tacgatgttt gaaaatgtta tgcaaaaaga gcaaccgact
    14341 catgttctgg ttgcgttcga tgcaggaaaa acgacttttc ggacagaaat gtatgcggag
    14401 tacaaaggtg gaagatccaa aacacctggc gaattcaaag aacaaatgcc atatatacgt
    14461 gatttgttga caggactagg ggtacagtat tacgaattgc ccaattatga agcagatgat
    14521 attatcggta ctttggcaga aaaagtagac aaagatcaat ttgatgtcgt cgtgctttca
    14581 ggtgatcggg atttgactca attagctagt aaagaagtaa aagtggatat cactgtaaaa
    14641 ggtgtcagcg atatcgaaag ctatacacca gaacatgtag cggaaaaata tgatgggttg
    14701 acgccaaaac agattattga catgaaggga ctagccggag atgcctctga caatatcccc
    14761 ggtgtgacta aaatcggtga aaaaacagct atcaagctat tgaaacaata tggctcagtc
    14821 gaaggaattt atgaacatat cgatgaaatg aagcaaagca aaatgaaaga aaatctgata
    14881 aatgacaaag agcaggcttt cttatcgaaa aaattggcga ccatcaatac ttcagcgcct
    14941 gtggatgtct ctatcgactc attgaagtat gaagggaaaa atctcgataa actcgttccg
    15001 ttttataaag aaatggattt caatcagttc ctttctaaat tgaatatcgt agaagaagaa
    15061 gtaaaaatgg atgatatttt gtttgaagtc gttcatgaat tcaaagagga aatgtttact
    15121 acggatatgg cgttgtacgt ggaaatgatg ggcgacaatt atcatacaga ggaaatcgtc
    15181 ggagtagcgt gggggacaga aaagaaaatt tatgtcacga acgatctatc tgtattcgaa
    15241 aaccgagcgt tccattcttg gatcactgat ccgactcggc tgaaaaaagt atatgatgcg
    15301 aaacgaactt atgttgcttt aaaccgctat actggaaaaa cgaaaggaat cgattttgat
    15361 gtcttgctag gtgcatacct tctcgatacg aatgaaaaaa gcacagatat cgaaggaata
    15421 gctgctcatt acggatacaa cgatatccaa tcagatgaag ctgtatacgg caaaggggcg
    15481 aaaaaaggtt taccagaaga agaggaactc ttttttgctc atttagcgcg aaaagtagct
    15541 gcaatcaatg cgttgacacc aaaattgagc caagaactgg cagaaaaaaa ccaagaagat
    15601 cttttccgga aaatggaact gccattgtct atcattttgg cagagatgga aataagcggg
    15661 atcaaagtag atgcgactcg cttacaagaa atgaagggcg agttctctgc tcgcttaaga
    15721 gagatcgaac aaaaaatcta cgaagaagcc ggcgaagaat tcaatttgaa ttcaccaaag
    15781 caattaggag tgatcttatt tgaaaaaatg ggattacccg ttatcaaaaa aacaaaaact
    15841 ggatattcca cagccgtaga tgtactggaa cagcttcgtg aacaagcacc aatcgtagag
    15901 gatatcctca cctatcgtca aattgcaaaa attcaatcta cctatgtcga gggactgctg
    15961 aaagtgatcc aatcagatgg aaaagtgcat acccgttatg tccagacatt gacccaaaca
    16021 ggacgattaa gctctgttga tcctaatttg cagaacatcc cgattcgatt agatgaaggg
    16081 agaaagatac gtcaggcatt tgttcctaga gaaaaagact ggctgattta ttcttctgac
    16141 tattcacaaa tcgagttacg cgtacttgcg catatctcaa atgatgaaca tttgaaagct
    16201 gccttcctag aaggtcaaga tatccatgcc agcacagcga tgcgtgtatt tggaatcgaa
    16261 aaggcagagg atgtcacacc aaatatgcgt aggcaggcaa aagcagtgaa ttttggtatc
    16321 gtgtatggca tctctgatta cggactttca caaaatctag gaatcagccg aaaagcagca
    16381 cagcaataca tcgatacgta ctttgaaaaa tacccaggtg tcaaagaata tatggaaaga
    16441 atcgtacgcg aagcaaaaga ccaaggctac gtcgaaacgc tgtatcaccg ccgtcgatat
    16501 ctgccagaca tcaattcaag aaattacaat atccgttctt ttgcggaacg aacagctatc
    16561 aatacaccaa tccaaggaag tgctgctgat attttgaaaa tcgcgatgat cgaattggat
    16621 aaacgattaa aagaaacagg tcttcaagct acgatgctgt tacaagttca tgatgaactt
    16681 gtcttcgagg taccggaaaa agaacttgaa tcattagata aattggtgaa ggaagttatg
    16741 gaacaagctg tttctcttca tgtaccactg atcactgaca gcagttgggg aaaaacgtgg
    16801 tacgaagcaa aataaacatt gtttaagaca gagcaatata atgaaggtga aaagaaaaaa
    16861 tgccagaatt accagaagta gaaaccgttc gtaaaggatt ggaacggtta gtagtaggaa
    16921 aaacgattca aaaagttcag gtcttgtggc ccaagatcat cgagcaacca gagacaccca
    16981 tctttgaggc ttctcttgtt ggcgaaacca tccaatccat tggacgtaga gggaagtttt
    17041 taatttttca tctcgatcat tgtgaactga tctcacactt gcgaatggaa ggaaaatatc
    17101 aatttacgaa agaaaataca ccaatcgata aacatacgca tgtacttttc ttttttgagg
    17161 atggaagcca acttcgctac aacgatgtac gaaaatttgg tcgtatgaca atcgtcgaaa
    17221 aaggagcaag tgcaacttac cgaggaatca tgaaattggg acctgagcct ttgccagata
    17281 gcttcttatt agcagatttc gcaaatggac tgaaaaagtc ccacaaagca atcaagccat
    17341 tgctgctaga ccaacgtcta gtgactggtc taggtaatat ttatgtagat gaagcattat
    17401 gggaagcgaa gatccatcca gaacaaccgg cgaacacact gcgccccaaa gaggtagaac
    17461 aattacgtct atcgatcatt gatgttttgg atcgtgcaat agaagccgga ggtaccacga
    17521 ttcgcagtta tttgaatgca ttaggagaat caggcggatt tcaagtagca ctgcatgtct
    17581 atcagcaaac tggcaaacca tgtatacgtt gtggaacgcc gatcgtgaaa acaaaagtgg
    17641 cacaaagggg gacgcactat tgtccgaatt gtcaaaggct aaaaatcgca aagtaggatt
    17701 tgttttaggt ttgacgggtg gaatagctac cggtaaaagt acggcagcca aagtctttca
    17761 gtctcatgga tttcctttga tcgatggcga tgtgattgcc agagaaactg ttgagcctgg
    17821 aacgcttgct ctgaggaaaa ttgtatcagt gtttggccaa gaggtattgc agcctgatgg
    17881 gcaactagat cgagggaaac ttgggatgat tgtttttcca tcaaaggaaa aacggcaaaa
    17941 acttgatcag ctattagatc cgtttatccg aaaagcgata aaagatcaaa tcgcctctct
    18001 aagttccaat catcctttag taattgtaga tatcccattg ctctacgaag gtcattatga
    18061 ccactatatg gatgcagtag ctgtcgttta tactacgcca gaaactcagc ttcagcgttt
    18121 gatgaaacgt aatcaactga cgctagaaca agcgcagcag agaattagta gtcaacttcc
    18181 aatcgaagaa aaaaagcagc gtgcggatat tttgttcgac aacaatggaa caaaagaaaa
    18241 acttgtggga caaatcgaaa attggctttt tgctaatcgc tttctttgag tatctgtgtc
    18301 aattttgcta tcctgagtat atagaaagtg agggatagcg atgggatcat taaaaaatca
    18361 aaaggataaa gtcgtaggta acgcaaaaga aaaaatcggc gagatgctca ataaagaaaa
    18421 tctaatcgat gaaggtcgta cgcaatcaga aacagcagaa gaaaaagaaa agcagtacca
    18481 agcaagaatc gatgaaatag aagaccagca ggcagaagaa gaagcgaagc ggcaagattt
    18541 gactcaagaa cgaaaaaatc cgacacctga tcgtagtaaa acaccgctcc cagaaagtga
    18601 ccacattccc gatgagcaaa aaatcgagga acagcgaatg aaacactata caggaatcga
    18661 agagaagtta taaaatagta aaaaatcgag atgtgacttg aagcagatga gaaggtgttc
    18721 atctgcttca agttttttgt gctagatgac taaatcagct tttgtgtaag ttatttctca
    18781 gtaatcaagg tgatcagtat tggaaaattg aaatttagta aggacagggg acacaattac
    18841 gcatgaaagt agaaaaaatt gttcttatat ccgtgttttt tgggtaggac agtcgcgttt
    18901 gtgatttgtt tcgtgttata atacaactag ttgtaaagaa agacacatcc tttatataaa
    18961 atgcgatgca agaggtgaga caaaatgaga tgtcccagat gtcaacataa taattcacga
    19021 gtaatcgaca gccgtcaagc agatgatgga cgtgcgatcc gtcgccgtcg cgaatgtgag
    19081 aattgttcgt tccgattcac tacatttgaa cgaattgaag ctgctccgtt attagtaatc
    19141 aagaaaaacg gggaacgcga agagttcaat cgcgataaaa ttttaagagg actcatccgt
    19201 tcagcagaaa aaagacctgt agctatggaa caaatggaac aaatcgtcaa ccatgtcgaa
    19261 aaccgcgtac gcagtctcgg agaaaacgaa gtatcgacga atctaattgg tgaatatgtg
    19321 atggaagatt tagttgattt agacgaaatc gcttatattc gttttgcaag tgtttatcgc
    19381 cagttcaaag atatgtctgt tttcttaaaa gagttgcaag atatcgtgga taaagcaaaa
    19441 gaaaataata aagagaataa taaagaaaac aattaagaaa caaaaaggag gcatttcctt
    19501 ggaaaatgct tggaaagagc ttcaaccaaa aaatatttac caaggaagaa aagcggctcc
    19561 gctgagagaa gacagcaaga acgccttgct gtatctttat cagccactaa tcggcggaga
    19621 agccttgagt atctatttca ccctgttatc agaaatctct caagaatctg gcaaaggtcc
    19681 tgaagggatg catgcagatc tgcttagtag tctaggctgc ggtatcccgc aattttatga
    19741 tgcaagaaaa aagctagaag gaatcggctt actggatgtt tattttaaag aagatagtga
    19801 tctgggacct cgttttattt atgagctgtt agaaccactc attcctaaag catttttcaa
    19861 agactctgtt ctggcttttc ttcttctgga aaaagttggt gaacgacgat tccagaagct
    19921 tacccaattc tttgaaccta aaaggcatac agcagaggga tatcaaaaaa tcacaaaaaa
    19981 atttttagat gtttatcgct tcaatgaagc agtgtacgct gctaatcaag aaaacatcga
    20041 atcgctgaat aaatcttttg aagaatctga atcagtagca ttacttgaag cagattcgac
    20101 attcgattgg cagtttttga atgattggct ggcaaaaaaa catattgcaa gtcttgatga
    20161 tccaatcatc aaacaagtcg acatgtatca tcagctttac ggtttcgatg aattgacact
    20221 tggcgaactg atcagtcaat cttatgactt tagtgaacaa aaagtatctg aaaaagaact
    20281 gcaaaaagcc attttggcaa tgcagagacg tccggtgaag caaacaaaag aaacagttgc
    20341 ggtttctgaa aacagtaatg aaccctcgtc aaacgaacat ttgacaaacg cagcgcttca
    20401 gctgataaaa gaagcacaga gcattccacc gatgaaatat ctggaagcaa tcaagtcaga
    20461 aaaaggcgga tatgtttcta aacaagaaaa ctggttactg caagatcttg tttctcaatc
    20521 aggtctttcc agtagcgtga tcaatgtttt gctgaattat gtactagtga tcaagaataa
    20581 tgctagctta aatgccagtt atgtaaatac agtggcaaat gaatgggcac aaaagaaaat
    20641 cgccacagca gaagaagcga tccagcacat tcggcaaata agtaatcaag caaaacaatc
    20701 aaaacaaaag aagcaaacaa attatcaaac tggcaagcga aatgttcgac gggaaaagct
    20761 tcctgattgg gtcaaccagc caaaagatga aaaacagatc agcagcgaga aaaaagcaga
    20821 gattgaccgc cgttttaaag aatacctggc aaaaaaggag ggtgatatct agtggaagac
    20881 gtaggcaaag aattgaagag gatcatcagc aaacgcaatt atcaagaaaa gtatgctgaa
    20941 atgatcgctg aagtcttaaa tgatgcggat gttcaagctt ttctaagcgc acaccaagaa
    21001 gaattgacac aagcggatat tgaaaaaagt tacgctaaat tgtatgaatt cgtgcaagaa
    21061 aagcgtaaat atcaaaaaaa cgatccaacg atgatcgctc ccggctatga gccgaagctg
    21121 acgttgaatt ttcattatgt ggatgtcact tatgtaccaa cggaagcatt gttagcacgg
    21181 caaagacaag aagaaatcag aagccgagta aaagcaatgg atatgccaaa agatattcaa
    21241 tcggcttctt ttgaaaactt tgaacgtaca gatggacgag gatatgcgag cttagaagcc
    21301 ctagattttg tagagaaata tactgctgat ccaaaagggt atcataaagg actgtatcta
    21361 gtaggaagtt ttggtattgg aaaaacatat ttattaggag caacagcgag agaattagcg
    21421 attgctggat tcaccacgac gctagtccat tttccgacat ttgccgttga gatgaaacag
    21481 gcaatcggaa aagatcaagt ggcagaaaag ttagatgcag tgaaacgttc acctattttg
    21541 atgatcgatg acattggtgc agatgcgatg agtagttgga tacgagacga agtattcggt
    21601 gttatccttc aatatcggat gcaggaacag ttaccaacct tcttctcatc gaattttaca
    21661 atggatgaat tggaacagca tttgtccgtc actcaacgtg gagatgaaga gccgctaaaa
    21721 gcgaaacgga taatggaacg catacgttat ttgacaaaag aaatcgaaat gactggtcgc
    21781 aaccgacgca atggttgaac agttgacaag tccaactgac atttgaccta gtagtaaaag
    21841 caggtcagcg aatcattata tggacagaaa tgaccggaga caatcaccgc aacaagtaat
    21901 gcagaaactt aaacggagga ttttttatga gggctatttt ttctagtata tctgtagttg
    21961 gtctattttt tcttttagca ggctgcggta gcgcagatag caacacaggt tctgttgatt
    22021 cgattgatgc gggtcaaaca gtaaaaacaa gcagtacgaa aacggagaga caaagcatat
    22081 ttgtcggaac gattgaaagt attactcaag tagaagcaga agaagaaacg cttcagttga
    22141 aactcgtcga tgtcatggcc aaagaagatc cagcgaatat gggaacgtca ttcagcaatg
    22201 acggagtgat cttaaatgct actgctgaaa aaataagcgt ggacataaat gaattgaaaa
    22261 tcggtgacaa agtccaattt acattagttg agatgccgat catgacgatg tctatcccac
    22321 cgcaagttcc tggagattcg attattgatg tttcattgaa caaataacca ataacatttt
    22381 ttcaaaaata agaaaatgtt gttttataga acgaaaaggt atatttttta aggagatatg
    22441 ggaatttttt tattcctagt ctccttattt tatttggaaa atgctataat tatctagaag
    22501 gagtgaagct atgaaccatt tagatttatc tcagcgctta gccgcagtgg gtgactttgt
    22561 ccctgaatcc gctcgtttag cagacattgg atcagatcac gcttacttac ccgttgcatt
    22621 gatattaaga ggaaaaatcg aatatgcagt tgctggtgaa gtcgttcaag gtccttatga
    22681 atcagcacgt aagcaagtca aaaaagatgg attagaagac cgtatcaccg tacgccttgc
    22741 aaatggtcta gatgctatcc agccacaaga tggtattaca gctgtcacga ttgcgggtat
    22801 gggaggttca ctgatccgag atatcctaga agctggcaag ttgaatcaac gcttaagcgg
    22861 acaagaacgt ttgatccttc agccaaacat cggagagcgt acattacgat cttggctgca
    22921 agaaaatcag tataaaatcc aatcagaaaa aatacttgaa gaaaataaaa aaatatatga
    22981 aatcattgta gccgaaaaag aacaaaaacc agttacttac aatgaaatgg agctgatgtt
    23041 cgggccattt ctgcttgaag agaaaagtga gatcttcaaa tcaaaatgga caagagagct
    23101 tcaacaaaga aaagctatcc gtgaacagct caaacaagca gctaatcaag aggcacgtca
    23161 aaaacaaatc gagcaggaaa tccaaatgat tgaggaggtt ttaaagccat gacaatcagc
    23221 ggacgcactt tcatccaaaa gtttgaaagc tattgtcctc agtggctagc tgaagaagga
    23281 gacccagtcg gactgcatat cggaacattg gataagccaa tccaacgagt aatgatgaca
    23341 ttagacgttc gtccagaagt ggtcgaagaa gcaatcaaga aaaaaatcga tttgctgatt
    23401 gctaaacatc ctccaatctt tcgtcctgta aaacggctgg ttactgatca gccacaagaa
    23461 aaaatgtatg cagatttgtt gaaacatgat attgccgtct atgcagcaca cacgaacatg
    23521 gatattattt gggatggact gaatgactgg ttttgtgaac ttctcggtat tgaagtggag
    23581 agttatttag taaagactca tgagattcat tataaaaaac tggccgttta tgtacctgtg
    23641 gatcatgcac agaaaatgcg tgaagtatta gctgcagctg gtgctggtac acaaggtgat
    23701 tataccggta caagttttac ctcgattgga cacggtcgat ttactccgga agctggtgca
    23761 caaccagcga ttgggaaagt agggaagaca gaacaagttc aagaggcaaa agttgaggtt
    23821 attttgccgg aaactatcga aaaacaagtg attcaagcca tgcggtcagc tcatccttat
    23881 gaggaacctg cttatgatct tttcgctata gatgaaccag tcgaaatgtt tgggttagga
    23941 cgagtaggtg aattgccgca agaaatttct atagaggcat ttgtagaaca agtaaaagaa
    24001 gcgttccaac tagatggact gagaatagtc cagccaaaaa atgcaaaatc ctctgtgaag
    24061 cgcattgcga tttgcggtgg aagcggtgaa aaattttatc cgcaagcaat cgcccaaaga
    24121 gcagatgtct atatcacggg agatatctat tatcatacgg ctcatgatat gcaaagcgct
    24181 gggttgattg caattgatcc cggtcattat attgaatcgt tatgcaagca acgatttatc
    24241 gagaaatttg aatcttggaa acaagaagaa aattgggata ttaacttttt cgtttctgaa
    24301 acaaatacaa acccatttca atttaattaa aaggagagaa tactatgtac gaaaatcttt
    24361 tgccacgttt tttacgatat gtcaaaacag aaacacgctc cgatgcaaca agcaaaacga
    24421 ctccatcgac tcagacacaa gttgcttttg cgcaagaatt gaaaaaagaa ttagaagagt
    24481 taggcttagc tgatgttttc tataatgaag ataacggatt cgttatggga acattaccaa
    24541 gcaatgttga tcatgaagtg cgttccattg gatttatttc ccacatggat acagcagatt
    24601 ttaatgcagt aggagtcaat ccacaaatca ttgaaaacta tgatggcgag tctacgatcg
    24661 tactagatgc ggaagaacgc tatacattga atacaaaaga ttttccaaat ttgaaaaatt
    24721 atgaaggcca cacattgatc acaacagatg gcagtacatt actgggagca gatgacaaat
    24781 cagggattgc ggaaatcatg actgcaatgg agatcttgat caataatcct tctatccctc
    24841 acggtaccat caaagtggct tttggtcctg atgaagaaat cggtgtcgga gcggataaat
    24901 tcgatgttga gcaattcgat gtagattttg cttatacagt cgatggtggt cctttaggtg
    24961 agttgcagta tgaaacgttt agtgctgctc aagcaaatat tacgatccaa gggaaaaatg
    25021 tacatccagg gacggcaaaa gatacaatga tcaatgctct acagttagca atcgattttc
    25081 ataatcagct tccagccgat gaagttccag aaaaaacgga aggatacgaa ggtttcttcc
    25141 atttgatggc attggatggg aatcctgaag aagcttctat gagttatatc attcgcgatc
    25201 atgatcgtga aaaattcgaa gcacgcaaag cacaaatcac acatatccaa gaagagttga
    25261 atcagcgatt tgatcaagaa cgaatcaaag tagatatgta tgatcaatat tacaacatga
    25321 aagaaatcat tgaaaaagat atgagcatcg tggaattagc gaaacaagca atgatcgagc
    25381 ttggtatctc tccagtgatc gaaccagttc gtggaggaac agatggttca aaaatctctt
    25441 acatgggtat tccgacgcca aatctatttg ccggaggaga aaatatgcac ggacgattcg
    25501 aatttgtttc tttacaagta atggaaaaag caacagacgt gattgtaaaa attgcagaat
    25561 tgaacacaaa gagttaaaat cgaaaaagaa cagcttatga caaggcgttc gtcccaatga
    25621 tggaatgtca tctgtctaag gctaaagggc aggacaatgg aattggttgt cccgccctct
    25681 tttttgaaga gtaaatagga ggtaaagaat ggaaaagctg ctagtttttt ataacaaaag
    25741 ttcagggaaa gatgagggtg agaaattagc acgctggttt caagattatg taaaagaaaa
    25801 gcagccagaa ttatatgtag aactagctga aactggccca tcgattgatg atgcggaatt
    25861 agtcaagaaa gcagaagcca tagaagctga tactttagta attataggtg gagacggcac
    25921 gattcatcat atcgtccaag cgttccaaaa aacattatct caataccatg tcggattact
    25981 tccaggtgga actgtcaaca accttgcacg tgttcttgag attcccctca aaaaagaaga
    26041 agctgctgac gtgatcctgg aaggcaatgt acggaaaata gactatggtc aagtcaatga
    26101 tgaagtcatt atctctacac tgacaatcgg aatcttagca gatacagcag tttggatcag
    26161 tcaagaagaa aaacagcgtt acggctcgtg gatctttatc aagaagtttt ttaagttatt
    26221 gctgaagaag aaaaaatatc atttggattt agaaacagac gaggagcatt ggaaaggaaa
    26281 atcccaactg ttgacagtca cgatgtctaa ctccgtgggc ggttttacaa attttgacga
    26341 ctcagcgacc ctagatgatg gaaaatttca tgtaacgatc gtaccttcct tagatatttt
    26401 ccgatttatt ttttatttgc ctagaatcat gagaggcaaa ttctatacga tccccggcat
    26461 tacttatttc acagccaaac gcatgaaaat ccaagcgcag gaaaaatctg tgggcactcg
    26521 aacggatgga gacacgacag atgatcttcc agttgaatta tctgttgtat caaaaggatt
    26581 aacagtttat tcaccaaaga aagaagaaaa agcttagaaa tagtagttaa cagaaagttg
    26641 gaagaagagt aatggatgta ttgttacaca atcctttgtg gttttacctt ttcattgtga
    26701 atttttactt attttgtctg atggggtatg ataaacatca ggctagaact agcggctggc
    26761 gtgttcctga gaataatttg atatttgtca gcctgttagg cggtggtttg ggaggattga
    26821 tctctcgaaa agttttccat cataaaactc ggaaaaagaa gtttactata ggattcgctg
    26881 caggtgtact tgttgatttg attctcattt attttttcca ttaaggagca ggagatgtcg
    26941 tctaaaggaa cgaaaactaa aatcattctg aatctgcttt tgctgattgt gatttttgcc
    27001 attatttatt atttaatcaa tcaatcattt gcagatatct ttgccgaatt gctgtctacc
    27061 agtgttgttg ttttggcagc ggctgttata ttagggacag tttaccaaat cgctgaaggg
    27121 cgctcaatca aagaaattgc tgaacctttc gcgccgaatt ttacaacgaa agatgggttc
    27181 ttgacctcta gctatattgc gttttaccga atcgtttcgt ttggtacagg aacgctgatc
    27241 tcggagattt atttttataa caaaaaagga atgaagctat cccagggagc aggggtgaca
    27301 gcactccata tgattatgta caaactggca gtgatcactt atgcattagc tggcttggtc
    27361 atccaattct ctctttttta ttctaatggt ccatcaatga tctggtttat catcgctggt
    27421 atccttttga cgatcgtgat tgttgccttt ttattggcga tgtccgtcag cttgaatgtt
    27481 caaatatttt ttgtcagctt atcgaataag ttatttaaaa gtgagcgttt gcggaaatgg
    27541 gtggatacat gcaatcagca gatctactct ttaagggaaa cggtccaaac cattatccaa
    27601 gacaggactg cgctattgcg gatttatcta tggaatatgt taaaacttgt attttggtat
    27661 gtacttcctt atttgattct gattgaaaac catcctaaaa tagattttct actgaccatt
    27721 tcgtttatca gctttgcagt agtgctttca ggggttattc ctacgcctgc tggtattggt
    27781 tcctttgaat ttgtctattt gttattattc agaccagttg ttggaacagt tgacgcagtt
    27841 tcttcgcttt tactgtatcg tttcagttcc tttatcttgc catttctcta tggatttgtg
    27901 tatgtgattg ctgaacggcg aagagtgatc aaagaagaaa taatagaagt aaaaaaagag
    27961 aataaagaaa atcaagggta ggtcttttat aagatctacc ctttttatca aatcaaagaa
    28021 aaacgttatg gataagataa ttttacgttt gtttggctct ttttttatat acatacaatc
    28081 aattttgttt ttttaagatt tatataagtc gagaaaaaaa gtcaaaaaac tatgtgtata
    28141 ttttgaccaa aattgaccaa agcgatataa tagtttttgt aaagatcaaa gaaataagtg
    28201 atcttacctt taattaggag ggtatacctg tatgaatatt gagaaaatga ctacgacgct
    28261 tcaagaagcg attgctgaag ctcaacagat tgcggtcact cgaaaacatc aagatatcga
    28321 cattgcccat gtatggaaaa tctttttaca atctaatcat tttggtcgaa atttctatac
    28381 ggatgccgga ttggatgtcg aatcttttga acatgaaatt gatcgtctac tggatgagta
    28441 tccagttgtt tctggtggaa acgtccaata tggacaaaac ctcagccaga atttctttcg
    28501 attattgaat gaagcagacc aaatacgtga aagcttcggc gacgagtttc tttcaacgga
    28561 agtcgttatt ttagcgttga tgaaattaaa aaattatccg ttaactgttt atttgaataa
    28621 aaacggatta tctgaaaaag aattgcgtaa aaacatcgaa gagatgagag gaggagaaag
    28681 agtgacctca caaaaccaag aagaacaata caaagcatta gaaaaatacg gcgttgattt
    28741 agtacagcaa gtcaaaagcg gaaaaatgga tccaatcatc ggtcgtgatg aagaaatccg
    28801 tgatgtcatc cgtattcttt cacgaaaaac aaaaaataat cctgttctga taggagaacc
    28861 cggtgtaggg aaaacggcaa tcgttgaagg gcttgctcaa cgtatcgtac gcaaagatgt
    28921 gcctgaaaat ttaaaagata aaacgatttt ttcattagat atgggtgcat tgatcgctgg
    28981 agcaaaattc cgtggtgaat ttgaagaaag actaaaagca gtcttgaaag aagtcaaaaa
    29041 aagtgatgga cgtatcctct tgttcatcga tgaaatccac aatatcgtag gggcaggaaa
    29101 aacagaagga agtatggatg ccggaaatct gttgaaacca atgcttgctc gcggtgaatt
    29161 acatttgatc ggtgccacaa ctttagatga gtaccgtcaa tatatggaaa aagacaaagc
    29221 gttggaacgt cgctttcaaa aggtattagt caaagaacca acagtggaag atacgatcag
    29281 tattctgcgt ggattaaaag aacgttttga gatccaccat ggtgttaaca tccatgacaa
    29341 cgcattagta gcagcagcta ctttatctga ccgttatatt actgatcggt tcttaccgga
    29401 caaagcaatc gatttgatcg atgaagccag tgcaacgatt cgggtagaaa tgaactctat
    29461 gccaacggaa ttagatcaag ttactagacg tttgatgcag ttggaaatcg aagaagctgc
    29521 actgaaaaaa gaatcagatg atgcaagtaa aaaacgcttg aaaaatttgc aagaagaatt
    29581 agctgaactt cgtgaagaag caaatgcgat gaagatgcag tgggaaacag aaaaagaaga
    29641 agtcaattct gtttcagcaa aacgtgctga aatcgataaa gcaaaacacg aattagaaga
    29701 tgcagaaaat aactatgatt tagaacgcgc tgctgtattg cgtcatggta cgatacctca
    29761 attagaaaaa gaattgaagg aattagaagc taaagcaaaa gacagcgaga ttaagatggt
    29821 tcaagagtcc gttaccgaaa atgagattgc tcaagtcgtt ggcagattaa cgggtatccc
    29881 cgtaacgaaa cttgtagaag gtgaacgaga aaaactaatt aaattgaatg aaacacttca
    29941 taaacgagtg attggtcaag atgaagcagt tgatgcggtc agtgacgcgg taattcgttc
    30001 acgggctgga ttgcaagacc caaatcgtcc tctaggttct ttccttttcc taggaccaac
    30061 tggtgtcgga aaaaccgaat tagcgaaagc gttagctgag aacttgtttg actcagaaga
    30121 tcatatggta cggatcgata tgagtgaata tatggaaaaa catgctgtgt ctcgtctagt
    30181 cggagctcct ccaggctatg taggttacga agaaggtggt caattaacgg aagctgtcag
    30241 acgaaatcct tatacaatcg tcttgttgga tgaaatcgaa aaagcgcatc ccgatgtctt
    30301 caatatcttg ttacaagtcc tagatgacgg acgcttgact gattcaaaag ggcgagtagt
    30361 agatttcaaa aatacggttc tgatcatgac cagcaatatc ggttcacagt tactgctgga
    30421 aggcgtgact gctgatggaa cgattccaga agcagtggct gaacaagtca acacgctatt
    30481 acgtggaaac ttcaaaccag aattcttgaa ccgaatcgac gacacgattt tatttacacc
    30541 attgagccta gacaacgtga aaggaatcgt tgacaaaatg gtggcgcaat tggcacaacg
    30601 tctggaacat caagaaatcc tgctgacaat cagtgatgaa gccaaaacgt ggattgctga
    30661 aaatgcttat gaaccagcat atggtgcaag accattgaaa cgatttatca ctaaggaagt
    30721 agaaacacca ctagctaaag aaatcgttgc aggacatgtt atgcctaaat caaaagtaac
    30781 gattacttta cttgatggac aacttcattt caaaacagaa gagctagaag aaatcgtcta
    30841 atcttccatt gagactataa aagaaaaaag gggtctgaca caactagtgt cagacctcct
    30901 tttttcaaat aaacagtgtt ccagaagtag tttcttcgaa aaataagccg aaatttcttg
    30961 aagctgcccg cttctgtcac aacctctttt attaggtatt tttcactttt tctaatttat
    31021 aactttcaaa gtaacgctca tcttttttac tttgagacca tgcaaacaat cccattaagg
    31081 cagcacctat cgtatttgtc agcaagtcgc ccatcgtatc catcaatgct gctcgtccga
    31141 caaacagtgt gccatcagaa gcagcaaaac gttgaagatt catgcctaag aactggtcgc
    31201 ataagaattc ccagaactcc caaaacacgc cgcaaagtcc agcaaacgca aagccgaata
    31261 agaggaacaa ccaaggactg gtttttgata tctcagcatc tttcatcaag taaccaatca
    31321 atccatagcc aagagctgtc aggaccattg gactcaccgc atgtaagatt ttgtcccaaa
    31381 aagagatgat actgatcaaa tgcatccctg tgcctaaaaa gacagagatg aacaagaaaa
    31441 accagtaaaa caaaacgatt gctgctggta ttttaagttt gaaaactttc aaaatgactt
    31501 gtggcatgaa aatcaatgca atccctaata aagattccac gataaatgtg attccttgag
    31561 aagtcgaata tttttgtgtg ataaattcat aaaaattata gcagacaacg aatacaccaa
    31621 aagcaagtaa agctatttgc acctttttga tttctttaat cactgaaagt ccctccatct
    31681 aaaaatcttt ttacttctga aagcaactca atttctgaag aaaccacttt tccgttcaat
    31741 ttgatcaaac ctattgtata taagttcagg tagtgaaatt gattttcagc tgtttcttgt
    31801 agtgcagcga tttttttcgg gtgggttccg ccttgctgtc gagtatcagt atataagccg
    31861 atcactggga tgcctttcgc ataagccaca ccgatttccg atgctacccc ggcatcgatc
    31921 gtcagcccat ctagtaaagc aatcattaaa tcactagcca acactttttc tgtgtcagca
    31981 agagcaatca ttcgactgtc tgcataagca gttttgtcat tgattgcttc attttcttga
    32041 ggaagataga tcgaaagctc agaagagagc tctctgattt ctttaactaa ctgttggtta
    32101 tataacaaat cacttttagc aaacattggt gcggcaaaat aaattttcat ctaaacatca
    32161 tcctttttat caaaaataat cgctaaagta agcttccatg tttggaatga tacgctctag
    32221 tgacttcagt gtttcttcat ctgtttctaa ccgttcgatt cttgccaaat aagaagcacg
    32281 gcgataattc atcatcaagc aagtcaatgt ttggatatcc agtcgaacgg attttcctat
    32341 aggttcattt aagacacgga cttgtccctg ttcatcccat gttaaagcga agacaccatt
    32401 attccaagga gcgacaggat cttcaatgat gaaatgaaaa gcatcagctg ttccaacaaa
    32461 cggaaaccgt tgaaggaatt cttttacatc aacgatccga gccataaaat atggttcgat
    32521 ctgctctttg atctgactat cttcaatcaa aaatgctaaa ggctcatttt tataaatatc
    32581 tccatgtacc cagtagatca tggaaaaatg tgcggaaata aagttccata agccatttcg
    32641 agcttcttga tcaagataga acatttcttt tacgtgaaat atttcttcag ctacccagta
    32701 gaacagaaca cctcttggtt catggttgct atcgtagtag acagcggctg tgcgctcatc
    32761 ttcattttcg aaacgccaat actcttccca atgaaaatca ctgcggaata aagcaccatg
    32821 gttttgttgg gcaaatttgt cataaacgga aaatacgtct ggatgatcaa ctggtaatcg
    32881 ctcaaccata ccacttactg ggatttgttt aggtagctgt gtatctctga ttttaaagga
    32941 aagtttatca gacatgattt cccatccttt acgccgatag taggggatac tgtaaggata
    33001 caaataagag atccattgct tattcttgcg catattatcc aatgcaacga taattagatc
    33061 ttgcatcaat ccatggttag catattctgg ataagttcca actccagtca caccacccat
    33121 tttataacgt gcaccgtgaa tattgacttc acaaggatag atagcgattt gagagatcag
    33181 tttattttcg tggaaccatc cgaacacttt cgatacttcc aaaatcggtt ttttggaacg
    33241 gataaaagct cgtttattct caaacccgct ttcctcgatg tctgcttcag tcacttggaa
    33301 cacataggaa agcaattcat tgaattgatc gatatgttct tgagtgaccg gttttaattc
    33361 aagattttct ctaaaatctt tttgatccat tttttcatct ccatttatta gcttgataac
    33421 agtatagcaa aattctacaa aaaaaacttc tatagctgac taattaaaaa ctctttaaat
    33481 tactgctttt cgttggtata atggaaagtg ctgattatat gaaaagagga gacgctatgg
    33541 atataaagaa aatgaaagaa cgccaagagc agattcgtaa tttttcgatc atcgctcata
    33601 tcgaccatgg gaaatctaca ttggccgatc gtattttaga aatgacgcac actgtgacgt
    33661 ctcgcgaaat gcaagatcag ctgttagact cgatggattt ggaacgtgaa cgtggaatca
    33721 cgattaaatt aaatgctgta gaactcaatt acacagcgaa aaatggagaa acatatatct
    33781 ttcacttgat tgacacccca gggcacgtcg acttcaccta tgaagtctct cgaagccttg
    33841 ctgcctgtga gggtgccgtt ttagtggtcg atgcagcaca aggaatcgaa gcacaaacat
    33901 tggccaatgt ttatttagcg ttagacaatg atttggagat cctgccagtc atcaataaaa
    33961 tcgacctacc cgcagcagat cccgaacgtg tccgtcaaga aatcgaagac gtaatcggaa
    34021 tcgatgctag tgaagctgta ttagccagtg ccaaatcagg gattgggatt gaagatattc
    34081 tcgagcagat cgtggaatat gttcctgcac caagtggaga cttagatgca ccgctaaaag
    34141 ctttgatctt tgactctatt tatgatagtt accgcggtgt tgttctaaat gtccggatca
    34201 cggacggtat ggtcaaaccc ggagacaaaa tcaaactgat gagtaatggc aaaacatttg
    34261 atgttacaga ggtcggggtc ttctcgccaa aagcagttgc tagagacttt ttgatggtcg
    34321 gtgatgtcgg ttatatcaca gcaagtatca aaactgtcca agatacacga gtaggggata
    34381 cagtaacgtt agcagacaat cctgccgctg aagctttacc aggttaccga aaaatgaatc
    34441 cgatggttta ttgtggtctt tatccaattg atacttctcg ttacaacgat ttgcgggaag
    34501 ctttagaaaa actgcaattg aatgatgctg ctttacaatt cgaaccagaa acctctcaag
    34561 cattaggttt tgggttccgc tgtggattcc tcggacttct tcatatggat gtcgtccaag
    34621 aacgcttgga acgggaattc aatctagaat tgatcacgac tgcaccatcc gttatttacc
    34681 atgttaataa aacggatggt acgactgcta cagtagataa cccggcagat tttcctgaac
    34741 ctgtaacgat tcaggatgta gaagaaccat tcgtcaaagc acagatcatg gtcccaaatg
    34801 attttgtcgg tgcagtaatg gaactttctc aacgaaaacg gggagaattc attacgatgg
    34861 attatttaga tgattaccga gtaaatgtcg tctataatat tccgctttct gaaattgtct
    34921 ttgacttttt cgacaaattg aaatcaagca cgaaaggcta tgcctcattg gattatgaaa
    34981 tgtctggtta tcaaaaaagc aagttagtga agatggacat cttgctgaat ggagaaaaag
    35041 tcgatgcatt aagctttatc gttcaccgag actttgccta tgaacgtgga aaagcaatcg
    35101 ttgaaaaact gaaaaaacta atcccacgcc aacaatttga ggtgccaatc caagcagcta
    35161 ttggacaaaa aatcgttgcc cgttcagaca tcaaagcctt gcgtaaaaac gtcttggcta
    35221 aatgttatgg tggagatgtt tctcgtaaac gtaaactctt agagaaacaa aaagaaggga
    35281 agaagcggat gaaacaaatc ggctccgtcg aagttccgca agaagccttc atggccgttc
    35341 tgaaaatgga cgaggacgaa ccgaagaaat agcaagggtt tgtttgaaaa cagtgtaaaa
    35401 aataaaccat gtttacatgg atttgccacc ggtttgccac cggtggtttt tttcggtggc
    35461 aaaaatcaag aattctttct aagattttcc atgatatcga ccgtttcatt tctcatctta
    35521 tctgttacat gcgagtaagt atccatcgta attgaaattc tgctgtggcc caatcgttca
    35581 gagatttctt tcatttttgc gccattttcg agaagaagtg tagcatgagt atgtctgaga
    35641 gaatggaagt taaaagagag ggagagtgca ttcgatatcc tcctagtatt ccatttcact
    35701 acacttggcg taactagctc accgtcttcc ttcgtacata ctgcatttga atcaatgtag
    35761 agctttccat acttcattcg attttctaat tgttgtttct tatgtttttt cagaattgct
    35821 agcaaggttt gtccaataaa aatcgttcga ttggaactgc ttgtctttgg cgtaccatat
    35881 acccatgcgc cgtcattctt taccatttgt ttctctacag taattgttcc atttgaaaaa
    35941 tcgacatcat cccacgtcag accacaaact tcgccaacgc gcattcccgt ataaaatcca
    36001 atattcaaag gaatatagaa aggatgtcct tcaggagtaa tttctagcat atgatcaaag
    36061 tcctcaagag aaatgatttt tagatctttt ttagtcgttg gtcgtgcttc atattttggt
    36121 atttttacat acagcatagg attttgcttg attaatcccc aaggataaac cgccatattc
    36181 agcgcattct taaggacaga gtgagtaata gtcattgttt tcttcgagta acccttttta
    36241 aattcagcat tgatgaaatt ttgtaaaaga gcaggggaga gatccgtaag ttttttcttt
    36301 cctaaataac cgtttatatg atttttgatg gtaaatcggt agttttcata ggtattgtat
    36361 tttagattta gtttaacgta ttcctccatc caaaaatcaa ggtattgttt tactcgagta
    36421 tccgtaccta aaaagtattg tcctgtttcg tcaatatctg ataaaacttt tcgtaaagca
    36481 gcttcggcct ctggtcgggt gtctccgcca actttctcca ctttttttct tgagccatca
    36541 tcattgatat cttcgccgtt tgtataatta agcgagacaa ataaaaaagc catttatgtt
    36601 acactc
//
LOCUS       DASHRQ010000024        36567 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.024, whole genome shotgun sequence.
ACCESSION   DASHRQ010000024 DASHRQ010000000
VERSION     DASHRQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.024"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(25..1521)
                     /gene="lysS"
                     /locus_tag="U2M88_001302"
     CDS             complement(25..1521)
                     /gene="lysS"
                     /locus_tag="U2M88_001302"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEM8373693.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="U2M88_001303"
     CDS             complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="U2M88_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEM8373694.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="U2M88_001304"
     CDS             complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="U2M88_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEM8373695.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3741..5852)
                     /gene="ftsH"
                     /locus_tag="U2M88_001305"
     CDS             complement(3741..5852)
                     /gene="ftsH"
                     /locus_tag="U2M88_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEM8373696.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="U2M88_001306"
     CDS             complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="U2M88_001306"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEM8373697.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="U2M88_001307"
     CDS             complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="U2M88_001307"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEM8373698.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8052..8522)
                     /locus_tag="U2M88_001308"
     CDS             complement(8052..8522)
                     /locus_tag="U2M88_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEM8373699.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8568..8993)
                     /locus_tag="U2M88_001309"
     CDS             complement(8568..8993)
                     /locus_tag="U2M88_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEM8373700.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9060..9329)
                     /locus_tag="U2M88_001310"
     CDS             complement(9060..9329)
                     /locus_tag="U2M88_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEM8373701.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9340..10935)
                     /locus_tag="U2M88_001311"
     CDS             complement(9340..10935)
                     /locus_tag="U2M88_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEM8373702.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="U2M88_001312"
     CDS             complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="U2M88_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEM8373703.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14488..15048)
                     /locus_tag="U2M88_001313"
     CDS             complement(14488..15048)
                     /locus_tag="U2M88_001313"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEM8373704.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15401..16375
                     /locus_tag="U2M88_001314"
     CDS             15401..16375
                     /locus_tag="U2M88_001314"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEM8373705.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16545..17945
                     /locus_tag="U2M88_001315"
     CDS             16545..17945
                     /locus_tag="U2M88_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEM8373706.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18023..18745)
                     /locus_tag="U2M88_001316"
     CDS             complement(18023..18745)
                     /locus_tag="U2M88_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8373707.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18824..19330)
                     /locus_tag="U2M88_001317"
     CDS             complement(18824..19330)
                     /locus_tag="U2M88_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEM8373708.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19453..21252
                     /locus_tag="U2M88_001318"
     CDS             19453..21252
                     /locus_tag="U2M88_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEM8373709.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21320..22894)
                     /locus_tag="U2M88_001319"
     CDS             complement(21320..22894)
                     /locus_tag="U2M88_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEM8373710.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23149..23658)
                     /gene="mreD"
                     /locus_tag="U2M88_001320"
     CDS             complement(23149..23658)
                     /gene="mreD"
                     /locus_tag="U2M88_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEM8373711.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23662..24516)
                     /gene="mreC"
                     /locus_tag="U2M88_001321"
     CDS             complement(23662..24516)
                     /gene="mreC"
                     /locus_tag="U2M88_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEM8373712.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24671..25309)
                     /locus_tag="U2M88_001322"
     CDS             complement(24671..25309)
                     /locus_tag="U2M88_001322"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEM8373713.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25310..25960)
                     /locus_tag="U2M88_001323"
     CDS             complement(25310..25960)
                     /locus_tag="U2M88_001323"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEM8373714.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25973..26872)
                     /gene="rsgA"
                     /locus_tag="U2M88_001324"
     CDS             complement(25973..26872)
                     /gene="rsgA"
                     /locus_tag="U2M88_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEM8373715.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27037..29106)
                     /gene="pknB"
                     /locus_tag="U2M88_001325"
     CDS             complement(27037..29106)
                     /gene="pknB"
                     /locus_tag="U2M88_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEM8373716.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29103..29843)
                     /locus_tag="U2M88_001326"
     CDS             complement(29103..29843)
                     /locus_tag="U2M88_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEM8373717.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29856..31214)
                     /gene="rsmB"
                     /locus_tag="U2M88_001327"
     CDS             complement(29856..31214)
                     /gene="rsmB"
                     /locus_tag="U2M88_001327"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEM8373718.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31214..32152)
                     /locus_tag="U2M88_001328"
     CDS             complement(31214..32152)
                     /locus_tag="U2M88_001328"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEM8373719.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32145..32636)
                     /gene="def"
                     /locus_tag="U2M88_001329"
     CDS             complement(32145..32636)
                     /gene="def"
                     /locus_tag="U2M88_001329"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEM8373720.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32651..35059)
                     /gene="priA"
                     /locus_tag="U2M88_001330"
     CDS             complement(32651..35059)
                     /gene="priA"
                     /locus_tag="U2M88_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEM8373721.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35312..35617)
                     /locus_tag="U2M88_001331"
     CDS             complement(35312..35617)
                     /locus_tag="U2M88_001331"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEM8373722.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35622..36236)
                     /gene="gmk"
                     /locus_tag="U2M88_001332"
     CDS             complement(35622..36236)
                     /gene="gmk"
                     /locus_tag="U2M88_001332"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEM8373723.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9885 a         7988 c         6706 g        11988 t
ORIGIN      
        1 attccttaga cagcttttat tcgattatct cattgttggg aatagcaaaa catcccgaat
       61 tgattgtgca tcagttagca acattactaa tctgtcgatc ccgatgccta gaccaccagt
      121 tggcggcata ccgtattcta aggcttccaa gaagtcttcg tctacaccgt gagcttcgtc
      181 atttcctagt tcacgttctt tttcttgcgc ttcgaatctt tctctttgat caattggatc
      241 gttcagttct gtaaaggcat tagcaaattc gcgccccact atgaatagtt cgaatcgatc
      301 tgtgaatcga ggatcttctg gatttttctt cgctaaaggc gaaacttcga ctggatgtcc
      361 gtatacaaac gtcggttgtg tcaatgtttc ttcaacaaat gtttcaaaga attcattgat
      421 aacatgtccg acacccatag cttcggttac ttctacattg tgtttttcgg ctaaagctaa
      481 tgcttcttcc aatgtcattt cttgccagaa gtctacacca gtttgttctt tgattgcatc
      541 taccatatgg atgcgtttaa agtcactttc taagtcgaca gcttgtccat catatgtgat
      601 ttttgctgtt cctagtactt tttcagctgc gttacggata ataccttctg tcagattcat
      661 tacatcattg aaatcagtat atgctgtgta tgcttctaac atcgtaaatt ctggattatg
      721 tgtagtatca attccttcat tacggaaaac gcggccgatt tcataaactt tttccattcc
      781 gccaacaatt agtcgtttta aatgaagttc tagcgcaata cgcaaatata aatcaatgtc
      841 cagtgcatta tgatgcgtga taaatgggcg tgcggcagct ccacctgcct cattgtgcaa
      901 tactggtgtt tctacttcaa tatagccatt gccatctaaa tagcgacgga tttcgctgat
      961 aatttggctt cgtttcataa atcggtcaaa gctttcgcga ttactgatta aatccaagta
     1021 gcgttgtctg tagcgttgtt caatattcgt taagccatgg tatttatctg gcagtggacg
     1081 aagtgctttt gacaaaatcg tgatttctga agctttgata gaaacttcgc ctgtgtctgt
     1141 tttcatgatt tgtccagtta caccaaagaa atctccaagg tctgcatgtt tgaatacttc
     1201 ataggcctca tcacctactt ggtcttttct tacataaatt tgaatttggc cttcacgatc
     1261 ttgtagatgc gcaaatccag ttttcccttt tcctcggcga gtcatcatac gaccagctac
     1321 actagcggtc aatcccattt ctgctagttc ttcttttgtt cttggatcaa atagttcatg
     1381 caattcttct gagttgtgcg tgcgttcaaa tcgttttcca aatggatcga ttccatcttc
     1441 acgcaattgt tccatttttt gacgacgtac aagcatttga tcatttaaat cttctacttg
     1501 atgttgttgt tcctgtgtca caaaagttcc tccattctca tgttaatctc tttcctataa
     1561 tgccaatgaa acacaaaaaa agcaagccga acttggaatt tcggcttgaa aattcacgaa
     1621 aaacttagaa atcacaacgt ttgtttcaat agtacttttt ccgctttttc tacaaattga
     1681 tccagcaggt cattgatttc ttgctgtttt tctgtctgat tgattgctac tttcactttc
     1741 gctgctcgag gaatcccttt taaataataa gccgcatgtg ttctgaattc tctacaagct
     1801 actttttctc ctttgagatc tgctaagcgt tgtaaatgaa gtttagctgt agcaattttt
     1861 tcggctggtg ttggttcagg aatcagttct cctgtttcga gataatgctg tgtgcggtga
     1921 atcatccatg gattgcccag cgctgcacga ccaatcatca caccgtctgc accgacatag
     1981 tcaagcatcc attttgcatc ttctggcgtt ttgacatccc cgttccccat aaatgggatc
     2041 gtcaaatgtt ttttcacttc tttcaatact tcccagtccg cttttccttc atacatctga
     2101 acccgtgtac gtccatgcat agcgatggca cttgctccag ctgattgtgc tgctaatgca
     2161 ttttctacag caaagacgtg ctcttcatcc caaccaatac gcatcttgat tgttactggg
     2221 atatttactg ctgaagaaac agcttggacc atttcgtata ctttctcagg atctaaaagc
     2281 cattttgctc ctgcttctgc tttgattact ttatttactg gacagcccat attgatatct
     2341 attatatcgg cagctgtgtt ttcttcgaca aattgagcag cttcaactag actatcttta
     2401 tttccgccaa atatctgtaa acttaatgga tgttctgttt cttcaatgtg gagcatctct
     2461 aaagtttttt tgtttctgaa atgaatacct tggtcactga tcatttcaca tacgactaag
     2521 cccgcaccaa attctttgac agtgactcga aatgctgaat ttgttattcc agccatcggt
     2581 gcaacaacga cacgattagg gatttccaca ttgccaattt tccatgtgtg ttccacttaa
     2641 atccctccta aggcttcttt cttcaatcca tctaattctt cttcgctata cttgtatttg
     2701 ttattacaga aagaacaaac cgcttctgca ccgtgatctt cgtctatcat tgcttggatt
     2761 tcttctgcac ctaacgcgat aattgcttct gcaaattttt cttttgaaca gtcacatttg
     2821 aattgaacag gcattttttc caaaatgttc aattcttctg taccaagcaa tcctttcaat
     2881 atttcttctg gtgtctgtcc ttcatctaag agggtagaga ttcttggtgt cgtttttaat
     2941 tgttcttcga tcttgctgat tgtttcttca tcggcacctg gcatgatttg gatcatgaat
     3001 cctcccgccg tcttgatgct gtcgtcagta ttgaccaata cactcagtcc aacagctgaa
     3061 gggatttgtt ctgagatagc aagatagtaa gtaaaatctt cagcgatttc tccagaaaca
     3121 atcggcgttt gcccagaaaa aggttctttt aatcctaagt cttttgtgac agtaaacatt
     3181 ccttgtgttc caactgctcc ccgcacatcg attttacctt cactatttag aggaagactg
     3241 agatgcggat ttttgatgta acctttcaca tctccctttc catttgcatc tactacaatt
     3301 cctgttgctg gaccatcgcc ttggattcga acagttagtt tgtcttctcc tttgagcgtc
     3361 gcaccaagta gcagactacc gaccaatgtc cgtcccaaag cagctgagga ggtgtgccaa
     3421 gtatcatgac gtctttgtgc ctccgctacg gtatttgttg cttgaacggc gtatgctcgg
     3481 acgaatccgt cgtgagcaag tgccttaacc aaataatctt tcatttgtta tgtactcctt
     3541 tttccgtttt taaactcata gtgaatcata ctagcaacta ctgctttttt caactttgga
     3601 atgcttcttt taaaaataat tcagaatcat ctagaaaaca ggacatcacc ctatttacaa
     3661 cttaaataaa gaaatctccc gaaaaccggt ttttgttccg gttttcggga gtcatatcag
     3721 atcaattgac ctatattttt ttatttgtat tgatcgtctg aatgattatc ttctgattgt
     3781 tgagaatctg aatcatgttc tgctttattt tcctcattca gttcatgttt tgcttcttca
     3841 aaatcacgtt tttcttccgc ttgtttttca gcgtcttttt cttccaaagc acgttttgct
     3901 tcttcaaaag ttttcgcttc tttttcgctt ggataatcat ttgactcagt accttcaggc
     3961 atcacaccgt gttcaaacaa tgacttgatt gctttggcat ccaatgtttc gaattccaaa
     4021 agtttttcag caatcagttt atgctgtgca cgatgagctt cgatgatttc tcgagcttta
     4081 tcatgagctt ccatcaagat ttttcgtact tcttgatcaa tctcgaaagc gacttgttcc
     4141 gagtaagctt ttgtttgacc atagtcgcgg ccaacaaaca cttgatggtt tccttcatat
     4201 tgaacaggac ctaaacgatc actcattcca tattctgtta ccatgcttcg tgctagagct
     4261 gtcgcttgct caaagtcatt ggaagcacca gttgattgga cattgaagat gatttcttca
     4321 gctgtacgtc caccaagtaa gcctacgatt tgttcaaaca tatcttcttt cgtcatcaag
     4381 aactgatctt cttttggtaa agcgatcata tatccgccgg cgcgtccgcg agggatgatc
     4441 gttactttgt ggacgacccg agcgcgactc aatactaatc cgacaatcgt atgaccagct
     4501 tcgtgataag caaccatttc gcgttctttt ttgctgatta cgcgatcttt tttagctgga
     4561 cctgcaatca cgcgatcttc cgcttcatcg atatcagaag catcgatttt ctttttattt
     4621 cttctagcag ctactaacgc tgcttcattc aagacatttt ctaaatctgc tccggcaaaa
     4681 cctggtgtct gttgagccac aactttcaag tcaacgtcat ctgccatcgg tttattccgt
     4741 gcatggactc gcaagattgc ttcacgacct tttacatctg gacgtccaac taaaatctgg
     4801 cggtcaaaac ggcctggacg tagtaacgct gggtctagta catcagaacg gttggttgca
     4861 gcaatgacga ttactccttc atttccgtcg aagccatcca tttctacaag caattggttc
     4921 aatgtttgtt cgcgttcatc atgtccgccg cccataccgg caccacgttg acgacctacc
     4981 gcatcaattt catcaatgaa aatgatagct ggtgcgtttt tcttcgctgt ttcaaacaaa
     5041 tcacgaacac gacttgctcc aacaccgaca aacatttcta cgaaatcaga acccgagatc
     5101 gagtaaaacg gtactccagc ttctccggca acagcttttg ctaataaggt tttacctgtt
     5161 cctggaggtc cttctaaaag tacacctgcc gggatacggg cacctaattc aacaaatcgg
     5221 cgaggatctt tcagaaattc aacaacttca actagttctt gtttttcttc ttccgcaccc
     5281 gctacatcag aaaaacgtac gcgattagct tttttgtctg cttctttggc ttttgattta
     5341 ccaaagttca tgacacggcc gccaccgcca ccgccgccgc cttgttgtcc catcatcatg
     5401 taaaagaaaa agatgatgat tacgattggc aagaagctaa gcaataggga aacccatata
     5461 ccgctgcttg attcttcttt tactgttgta gcaactttgt tttcttttgc cagttctgtt
     5521 acttggctca atgttgtatc atttggcaaa atgattgttg tgaaatgggt agtagtcgta
     5581 tctgttgaac ctaagatagc taacccaccg ctgttagcga cggtctgctg ttctttgtat
     5641 tcaccagtga tcttatatac accatttgca ggctgcactt caaattcttt gatcttgcct
     5701 gcttgtagtt gttctgtgaa ttttgagtac tcgatatctg acgatcgctg gccgttatta
     5761 ccaaaaatga aataaacgac cattaccatc gctaagatta cgaggacata atacaaggca
     5821 tttttcatca tgccgttgtt ctttttgttc atagttgccc tccctaagcg tcaatttaat
     5881 ttaacagttt tttatcaatc ctgaccattc tattgtacca taatttaatt gagatacagc
     5941 attaatttga ttgataaaca ctaggtttca atacaccgat gtacggaagg ttacgatatg
     6001 cttctgcata atccaagcca taaccgacca caaattcatt tggtacgttg aagccaacat
     6061 aatctgcttc gatatttact actcttcctt caggtttgtc aagtagtgta acaattttga
     6121 ctgattttgc tttgcggtat ttgaaaagat ccactaaata agcaagtgtt cttccgctgt
     6181 caatgatatc ttcaacgatc aacagatcgc gaccttctac attcgtatct aagtctttga
     6241 cgatttttac ttcccctgac gagactgttg cattcccgta gcttgaaaca tccatgaaat
     6301 ccaattccaa atacgtatcg atagaacgaa cgatatctgc catgaatggt accgctcctt
     6361 ttaagacacc tactactagg ggattggtgt tttggtaaat ttctgttaac tctttgccta
     6421 gttctttgca acgaacttgt atctcttctt gtgagatcaa aattctctca atatcctttt
     6481 ccaacatcag gttctccttc caattgcttc tttttgatat ttgtaaagaa gtatgtagtg
     6541 tattttatca gtttcttcac gaatactcaa atatgaaaag gcaaaaggta tcaagctata
     6601 tatatttctt ttggcatctg taatgatcca agcatgctgc ctttgtgaat taggtacttt
     6661 ctgatcgata aaatatctag atactttttt tgtgagattc tctgttaatt taatgcggtc
     6721 tcctgctttt cttttttcga catacagttg cacatcagca ggaatccaaa tgtcatgaga
     6781 aaatagagac catgaatctg ttgctttgat gtcccctgca ttttcaggtt tgaccagacc
     6841 aatccattct gtttcattca aatatattcc ttgttctgga atcagcaaca agcgctggtc
     6901 ttctgtctgg ctagttgctt gtttttcttt tttagagagt acaacaaaat gatactctct
     6961 tttcagtatc caattattgc caatactgag ttgccattgg ctttttttct gttgccactg
     7021 gtctaaaatc gactccattt gttgttcttt caaaataatt cctgtttttg agaatgcttt
     7081 gtcgaaaaag gagtaaaaaa aatagtatcg ttcagcatct gtcatttctt caatcatttc
     7141 tgcagaaaat cgaaattgtt cctcccactg ttcgacttcc ttttcgatca actgtcccat
     7201 atatttttga atgacttggt ctgcccattg gatctgttgc gaaaattgct ggaaatgtct
     7261 caacgcctgt gtattttctt gtttcaattg aggaactacc aaatgacgaa gccgatttct
     7321 ctggatatct aacaattggt ttgtctgatc ctcaaagtaa acaagctgcg attccgctgc
     7381 ataagtatat aggtcttctt tgctaaaact cagcaacgga cgaatcagtc tgcccgtagc
     7441 aaatggttgt acttctttga ttccagaaaa agtatttaac tgtcccccac gaacgatttt
     7501 cattaacacc gtctcgattt gatcatctcc gtgatgagca gtcatcaacg tgtcatattg
     7561 tccttcttcc atcacgcgag tgaattcttg ataacgaaag ttacgagctt ctgtttctat
     7621 ggcggatttg gctggatctt cccaaatact tacatataaa gggagattct tctcttcaca
     7681 atatgagcga agataggcag cttcttctgc tgattcttct cttaattggt gattgatgtg
     7741 gacgatacct aaagaaaaac cgatttggcg ttgcacttgt aaaagtaaat ggagcaaaac
     7801 catcgaatca acaccacctg ataccgctgc taaaattctc gatccttgtt gccagaatcc
     7861 ttgttcattc ccgtgccgaa taaatgactg ctgtaatagc ggtgcttttt tcccgccttt
     7921 ttcaacgttc atcctttcca caccctttct tcctaaaaaa aagagagcgg gaaaacgtct
     7981 aaaacgacaa ttaacctgcc ctctgttgat actatgaccc aaatagtcgc actatcatta
     8041 tttgcggctt tttagctgcg acgtccgcca cggccaccgc gctttccttc tgtgtttcgc
     8101 ttcaatgaag ataagcggtc atcgctatct ttcaaaaatg aactcatcaa tgaatcgaag
     8161 tcttcttttt gagctgaagg ttgactgcga gaaaaagatt ttttctgtgc tggacgattt
     8221 cggttgtcac ggggatcttt ttgttgatat tttttaggac ggctttcttc tccagattca
     8281 gcaggttgtt cctgcgcttt ccggatagat agtccgattt ttccatcatc atttactgaa
     8341 ataactttaa ctgttacttc atctcctaca cttaaaacat catggatgtc ttttacatag
     8401 ccatttgaca cttcgctaat atgaactaga cccgtttttc cagcacctag atcaataaac
     8461 gcaccaaaat ttgtgattcc tgatactttt ccttgcagtt ttgctcctac ctcgattgac
     8521 ataaaaaaaa tgttcctcct aattatttcc cttttttata ttcctgctta ttttgcagtt
     8581 gtagaactag ttgattgatc tgtttcattt gtcgagctgt ctgtatttga ttctaatata
     8641 ggataaactt tttcaccgtc ttttgagtaa aagaatttgc ttctggcaag tttagctaca
     8701 tactcatcat ctttcagcaa tgaaacatct tggtgcagct gatcaacgtc tgcgttgact
     8761 tctttcattt cagtatttgc ttcgactctc aattcgttca attgatgcat cctttgcatg
     8821 tcatggaaaa tctgcacacc tattatcgcg aaaatgacca aagcaccaac aaaaattgca
     8881 gccagtctgc gtcttcgaaa aatcagttga cgttgttgtt gctgaaattc agcatacttt
     8941 ttctttgcat attccgtatc tagcgcagcg attttattgc tttttttcgt cacttggatt
     9001 cgctccaatc tttttgattg tctttttcat tataccaata tcgtgaaagc ttgtctaatc
     9061 tatttctcta gtgtttctgt gcgattttca gaaataattt cgtacatttt ttgtgcatct
     9121 tcttttttgg ttgattcatg taattccgta attttcactt ccaacgtttt gttgccaaac
     9181 tggactttca aaatatcgcc tactttgaca tcactggaag atttcgccaa tttaccgttg
     9241 atttgaattc ttcctttatc agctacttct tttgctactg atcgtctttt gatgattcgg
     9301 gaaattttta aaaatttatc taatcgcata tcttattcct cactttttaa tttttttatt
     9361 taatcgcagg attttcttac caaaaggcaa cattagccat tctcgtactg taaacagacg
     9421 aagacgaatc actgcataaa tgaacacaga agccccgatc ccaactccta tcacgctgac
     9481 aaaaaaagca gtggaacgat gagttacagg accaaatact atattcaata caccataata
     9541 cagcagcagt actactgtca tacaaagaag gctgaacatc aacttccgtc caaaatgccg
     9601 ttctttccaa aaagcattta gctcttttgt ttcaattcga acaaaatacc aaagcgtcac
     9661 acctaatcct aaaagggtag acaggcttgc acctgctgtt cctaataaac cggtcagaaa
     9721 agaagtagtc aatcctttta ccaatagtcc ccatcctgcg ccttttaaag aagggcgaaa
     9781 cgagttcttg ctttgagcaa tactttgata agcttggatg actgcagtga aagcaatgga
     9841 aaaaacaaat aatacaagcg ctgcatttcc cgcataatct ttaaacaacg cataattgat
     9901 ataagggagc agcaaggcta atcccaaaga cgcagctaat gctaaagcgg tcgtcaatct
     9961 aagatagatt ttggctgtct gtaaaaactg acgatagatc cggttggtca aatgacgagt
    10021 caacgcaggc aggaatgtcg cacttaatgc agtggcaatc actaaaccta attgtactaa
    10081 aggctgacct cgatcataga ccccttttgc tatcttcgca ccgtattctg acagtccgta
    10141 aacttccaaa gcatttttta caaagaaaga gtcgatcagc tggaaaaaga taagcaatcc
    10201 actataaatc gaaaccagtc cgccttctac cagaaaacgc cggatcaagg gctttgacgg
    10261 ctgtttgaac aatggaaacc gtcggaagct taacgctcct ccatgaatct tttgttcata
    10321 gtagtgaagg atcccatatg cgcataaacc gccaaaaaca gctccactca ttgctgccgt
    10381 ccctgtttgg tacaccgtcc atccaagctt tctaaaagca aacgcactac agacaatcac
    10441 aaaaacacga acgaactgct caactacttg agaaacagca gttggttcca tcaagaatct
    10501 tccttggaaa ttccctcgat aaaaagatag accaggcatc agcaaaaagg taaaggaaac
    10561 tacttggatc agcggtttca attgtatgtc tcccatcatc caagcaatcc agctgctaaa
    10621 tgcaaagaca aacaaccaca aaaagacacc tgtccaaaat actaagggat aaagattttg
    10681 caaagcttgc tttcgctcga aaggattctt tctttctgca acgtactttg atatgaactg
    10741 cggaagaccg gataaagcta aagtcattgc tatcccataa attggataaa cttgctgata
    10801 aacataaaaa ccttcgtcgc cgactaagtt ttggagggga attcggtaaa gggcacttaa
    10861 tacttttgct atgaaagaag caatcgtcaa tacaaatgct ccttgcatga cgcgccgcat
    10921 ttctttatga tccaaatcat tgctccttcc tacgctgaca gatatttttg ctcacgcaac
    10981 gctttgacaa acttttggat ttcctgaagc catactgctt cttccatttg gttggggatc
    11041 gtcaagcgta tcaccatctg ttctttttca acgcctagac tggcttttag ttttgtcgct
    11101 gtcagtgctt cgaataactg ttccacacta tagcgttttg ttccaacttt acttagagta
    11161 aagactatat gcgcctcctg tttccttatc gtttctagga gcgctcgatc gccatccatt
    11221 ttgatctgcc caattgtcaa gagatgtgct acttcttctg gatattctcc aaaacggtcg
    11281 agcagatcat cttctagttc atctagcatt tcttgcgaat caagttcacg aattcgttta
    11341 tagatctcga ttttctgccg ttgatctgcc acgtatgttt caggtagata cgcatcgaca
    11401 ccgaggtcga tctcaacagt ggtcttctcc acttgactat tctttccttg tttacgagaa
    11461 acagcttcgc ttagcatttg agaatacata tcgaaaccga ctgcatcaat aaatccatgc
    11521 tgctgagcac ctaagagatt acctgcaccg cgaatggaaa gatcccgcat cgcaatcttg
    11581 aatccagatc ccagttctgt aaaatctttg attgcctgaa gacgtttttc actgacttcg
    11641 ttaaggatct tctgctgctc atacataaag taagcatatg cgacacggct gcttcttcct
    11701 acccgccctc gcaattggta taacgtggat aatcccatat aatcagcgtt ctcaacgaac
    11761 aacgtatttg cgttcgggat atctactcct gtttcaatga tggttgtagt gacaagaaca
    11821 tcatattgac cttcaataaa ttcaaaaagc gtattttcta attgcgcctc tgtcatttgc
    11881 ccatgagcat aaccgatccg tgcttccgga acgagttctt ggatttcttc tactttttgt
    11941 tcgatcgttt ctacccgatt atataaatag aatacttgtc cacctcgtcc catttctcga
    12001 tggattgctt cgcgaatagc cccaggattt ttttccataa catacgtttg gatcggataa
    12061 cgattcgcag gcggtgtttc gattactgaa agatcccgca cgcctaacat cgacatatgc
    12121 aaagttctcg gaatcggtgt tgcagtcaga gtcaaaacat ccacttgtgc acgtaactgt
    12181 ttcaatcgtt ccttatgttt tacaccaaaa cgttgttctt cgtcaatgat cagtagaccc
    12241 aaatcgctaa attcaatatc ttttgacaaa attcggtggg ttcctacaac aatatcaact
    12301 tgacctgtac gcagttgttc gatggtttct ttttgttgct ttcttgttct gaaacgactt
    12361 aatagcccga tatttacggg gaaaccttca aatctttcca gcatcgtttc atagtgctgc
    12421 tgtgccaaga tcgttgttgg aactaaaaag gctacttgtt tgctttcctt tatcgcttta
    12481 aaggccgctc gaagcgctac ttctgttttc ccataaccaa catcacctac gagtagtcga
    12541 tccattggtt tttctttttc catatcccgt ttgatctccg ctgcacttct caactggtca
    12601 tcagtttcag agtaggggaa ggcattttcg aattcttttt ggtaggcgtc atcaggtcca
    12661 aaagcatatc ctttttctga ctctcttgcg gcatataatt tgattagatc gtcagcgata
    12721 tcctcgattt ttgaagagac ttttcgcttg gttttcgtcc attcacttcc gcctagctta
    12781 ttgatgcgag gcgctttggc ttcagaggcg acatattttt ggatcagatt cagttgagta
    12841 acaggaataa acagtttgtc gtcgttttga tacaaaatag tcatatagtc ttggtgaacg
    12901 ccatctactt ctagcgtttc catcccaatg tatttcccga taccatgatt ggcatggaca
    12961 acgtaatctc ctgcttttaa ctcattatag cttttcaatc gctctgcgtt tgttactgtt
    13021 tgacgtctcg tttgtttttt cgtcgttttt tggaaaattt ctttttctgt gatagctact
    13081 attttttctt gaggaagttc aaaaccagcc tgcaaagctc cttcaactaa ttgtacctgt
    13141 cctctgatca atgcattttt ttctgtttct actactaata tgtcttcatc tcgaagcatt
    13201 tgttctgctt tgcgtattct gtcctttgtc ggaataaaga tcacaacagt ctgtttttgc
    13261 ttgcgccaac ggtccatttc tgttttcagc aatggcattt gtccgaagaa ctgttgcatc
    13321 gtccgatatt gaaaattatg gattgcttgg aaacgaagat ttcccatccc tttttggaag
    13381 agtgagaaaa aggttgtggt gaacttttcc ttctgcacga ttttttgtat atctgctgaa
    13441 aatgtctgct cagaaaacac ccgcaattcg ctgatttttt gagtctgcca ttctgccgct
    13501 tccctttcga tttcccgatt tgtttccatg attcgctgat aatcatcaac gactagtaaa
    13561 ctatttgaag ggaaataatc caagatcgtc acagattctt ggtaaagaag atctgagtag
    13621 aattttgctt cttctgtcgg gacacctgct tgccagcttg aagtcagctg accgaagtaa
    13681 tcttgcaaaa actcgcggtc tgcttcttct gtggtcgcag caagccgttt ttctagcatt
    13741 ttttgcattt gctctattcc atgttctaga tcctctggtc cgtagacttg ttcactcgtt
    13801 ggggaaaccc agatttcttc cgttttttcg attgagcgtt gtgtttctgc ttcaaaatac
    13861 cgcatagaat ctatctctac atcaaatagt tctacacgaa caggataggt tgtcgttaac
    13921 gggtaaatat cgataatgct tcctcgaata ctgaattctc ccggttttgc gaccatctct
    13981 tctcgtacat agcccatcaa agtcaattgg cgaggaagat tttccacgtc tatctcatca
    14041 ccaatatgcc aattcaattg cgcctgtagc caagttttct tattcggcag tcgtttgtgc
    14101 aaagatgcga ctggcaacaa atagatacct gctttttttt cttgaagtgc gttaagcgtt
    14161 gcaactctct cgcctcgcgc ttctggagaa gaaaaggcca tttcagcaga aagcacttca
    14221 tccactggaa ataaatggat ctgatcttgc ggaatcacat gttgcagatc ttctgctaac
    14281 tgattcgcat aatacaaatt tggcatcaga ataatcatgt ttttatttgt tttcttaaac
    14341 gctcgagcaa atagaagtgt tttcgctgag cccgctaatc ctgtgattag ttgtctaggc
    14401 attttttctt ctaaattgct gacccattct tttacttgcg gatcttttcc gactaattga
    14461 ataatatcca ttccttttcc tccttcctca attgaatcga ttcatcgcat ccacgaacga
    14521 atgcccttct atgagatatt cgactgcttt aacagattga cacatgcttt cttctatcaa
    14581 aggctgattt tctttgctga aaggactaag cacatgctgg actactgttt ttttcccttc
    14641 tggcctgccg attcccacct tgattcgatc aaagacattc gtattcaaat gggaaataat
    14701 acttttgata ccgttatgcc cgccggcact tcccttttga cggagccgga tttttcccac
    14761 tgccagatcc aaatcatcgt agattacgac taattcctct ggataaatcc caaaataagt
    14821 cattagtggg ccgactgcac gaccagattc attcatgaat gtttgtggtt tgactaaaag
    14881 gatcttttct ccattatgaa aaaattcggc aacttctgct tcgaacggat tttttttgaa
    14941 agtagcttga tgctcgcgtg caattttatc cacgaccata aagccgatat tgtgttttgt
    15001 atattggtat ttggtacctg ggtttcctaa cccgacgatc attttcattt ttgttttcca
    15061 cctaattcta aaaagcagca caaaaggctg gactgctaat tagcggtcca gtccatcgag
    15121 cttttttctt ttttatactt gtcaatgcat agtataacac aaaacaaggc gtttcggcta
    15181 aaagagaatc gttaataaat gataaagaaa ttaaaaattt cttcaaagtt taatgaaata
    15241 attgatatat aaaaatagtg aaataacgca catgtaagct tttacatata atacaacctt
    15301 gttatctgtt atattttata gccaaatagc gtgacatggg gcttgagaaa tgttagcata
    15361 agcatgggaa aaaacaaatc aatggaagga ttgatactaa atgactgcag ctgcagaaaa
    15421 aaaagatcat caaaaagtta ttcttgttgg agacggcgcc gtaggttcta gctatgcctt
    15481 tgctctagtt actcaaaata tcgctcaaga agtaggtatt gtggatatca atactgctaa
    15541 aactgaagga gacgcaattg acttgtctca cgctttggca tttacttcac ctaaaaaaat
    15601 ctattctgct acttacgcag acgcacatga tgctgactta gttgttatta cagctggcgc
    15661 accacaaaaa ccaggtgaaa ctcgtttaga cttagtaaac aaaaacctaa aaatcaaccg
    15721 cgatgttgtc acacaaatcg ttgaatcagg atttaacggt atctttttag ttgccgctaa
    15781 cccagtagat attttgactt actcaacttg gaaattctca ggtttcccta aagaacgcgt
    15841 aatcggttca ggtacctctc ttgactcagc tcgtttccgt caagcacttg cggaattagt
    15901 tgacgttgat gcacgtaacg tccatgctta catcttaggt gaacacggag attcagaatt
    15961 cccagtttgg tcacatgcaa atgttgccgg cttgcaaatc tatgaatggg taaaaaacaa
    16021 cccagaaatc gatgaagaag ctatggttaa cctattcttt ggcgtacgtg acgctgctta
    16081 cacaattatc gagaaaaaag gcgctacttt ctatggtatc gcagtagcat tagcacgtat
    16141 cactcgtgcc atcttagatg atgaaaatgc tgttcttcct ttatcagtat tcatgaatgg
    16201 cgaatatgga ttgaacgaca tctacattgg tgcacctgct gttatcaacc gtcaaggtat
    16261 ccaaaaagta atcgaaattc ctttaagtga cagcgagcaa gaccgtatgg ctgcttctgc
    16321 taagcaatta aaagaaattc tagatgaagc ttttgctaaa ctagacgctg aataatcttt
    16381 gaacatgacg gaaaacatct attcggtgtt ttccgttttt ttattttatc acccttatgg
    16441 tttctaaatt ttttcataaa gtagagataa ccttcgtctt ttcctctaaa ttatgctatc
    16501 atgagaccgt tccaccctaa acttttactt aaggagttgt cgatatgaca agatcttcgc
    16561 atcgaaaaaa atcgaaacat gttgctttgc tttcagttct tggtgttctc ttagtgctga
    16621 tcagctttta tacctatcgc agtacatact acacaaagca ttttttgccg aatacagaaa
    16681 taaatggaat aaatgtcagc aatctaacag tcgaaaaagc aaatgaaaaa ttaaaagaag
    16741 cttattctga taaaaaactg gctattaaag aagatggaaa agtctggcag gaagtaccaa
    16801 aatccgagct cggctacaaa gatgatttca cttcggagct ttctccgatc ctaaatgaac
    16861 aaaacggatg gacttgggga atgacttatg tctcagcggc agaaaaacaa gaaatagatc
    16921 ctgtcagtca aaaccaccaa aaactagaca ctaccgttca gacattgaca actaagctga
    16981 ctgatttgaa caaagaccgg acacctacta ctgatgcaac gatcgaaaaa gcaggtgact
    17041 ctttcgttat caagcctgaa gtcaatggaa atacgatcga cgtcgatgct gcggtcaaac
    17101 aattaaaaag tgcggtaaac tctggtaaag atacaattga attgactgaa ttcaaagaaa
    17161 aaccaaaagt aacatctgaa gacagctcac tgaaagaaca actcgcatcc atgaatgcta
    17221 tcgcaaatgt aaaagctact tacagtataa acggtgagac tttccagatc ccatcaagtg
    17281 atattatgag ttggctgacc tacaatgatg gaaaagtcga tttagataca gaacaagtac
    17341 gtcaatatgt aacagattta ggcacaaagt ataacacaag tacgaatgac acaaaattta
    17401 aaagtacaaa aagaggagaa gttacggtcc cagttggcac ttacagttgg acgatccaaa
    17461 cagacagtga gacagaagca ttgaaaaaag cgattctggc aggccaagat tttactcgtt
    17521 cacccattgt gcaaggtggc acaaccgctg atcatccatt gatcgaagat acgtatatcg
    17581 aagttgatct ggagaaccag catatgtggt attacaaaga cggaaaagtg gcccttgaaa
    17641 cagatatcgt ttccggaaag ccaactacac ctacacctgc tggagtcttc tatgtttgga
    17701 ataaagaaga agatgcaact ttaaaaggaa caaatgacga tggaacacct tacgagagtc
    17761 cggtaaacta ctggatgccg attgactgga caggtgttgg tatccacgat tcagactggc
    17821 aacctgaata cggtggagac ttatggaaaa cccgcggttc acacggctgc atcaacaccc
    17881 caccaagtgt aatgaaggaa ttattcggga tggtcgaaaa aggaacgcct gtattggtct
    17941 tctaacaaaa ataatatata aaaagaaagc acccgtacga ttggtacagg tgctttcttt
    18001 ttattatcta acaatgtgct acttacattt cagcagcagt aaagacttct gaaacatcat
    18061 catcgtcttc tagcttatct actagacgtt ccaattgctc tttttgttcc tcatttaaat
    18121 caatcgtcgt ttgtggaatc atcgtcaatt ctgcttgcgc caaagtgaac tctttttcca
    18181 actcatctcg tacagcagca aaatcttctg gtgcagtata aatctcgaat acttcaggag
    18241 aagtttgcag atcttctgcc cccgcctcta atacatcctc aaacatcgtc tcttcatcaa
    18301 cagtcaaccc atcgcgttca atagcgatat atcctttgcg gtcaaacaag taactgactg
    18361 aacccgtttc tcccagtgat ccaccgtttc gagtaaaagc tactcggaca tttgtcgctg
    18421 tccgattacg gttatctgtc aatgcatgga ctaagattcc tactccaccc ggaccatatc
    18481 cttcatacgt gatttcatcg taattcgttt catctacggc actcgtagcc tttttaatcg
    18541 cccgttccac attgtcgtta ggcatatttg cagcttttgc tttgtccaca acaagccgca
    18601 aagctggatt catagatgga tcaggaccgc ctgctttagc tgccatataa atttcacgag
    18661 aaagtttctg aaagactttc cctcgttttg catcttgggc attttttctt ccttggatat
    18721 tatgccattt actatgtcct gacatgattt ttcctccctt gttcttttga gaatacactt
    18781 ctattctagt actatttcac aaaaaaacaa agccacagcc aatctataaa cgcttcttga
    18841 atgccgcaaa aagcacacat aatatcacag cagtcagagc accgatcgaa tccaacatga
    18901 catcttggaa cataggtgtt cgtccaccgg tcatcatctg atggaattcg tccaaacctg
    18961 cgtaccctgt tgctgaaagc caagcaagga tcgctgtcag ccaccaaatt tttagcttgg
    19021 ggaaaacacc taaaaacaag ctgcctccca aaataaaata ggtaccgaaa tgtgcgccct
    19081 tccttaaaaa gaactctaaa aatttggcgt agcccagatg gtcgatactc accatacttc
    19141 caccataatt aaaacgtata cccgctaatg tgtctttaaa tggctgatta ggcagccagc
    19201 tttctaatcg cgaaacttgt gattgctgtc cgtaagtttg agaagaactg taaaataaga
    19261 ttataacgac tatcagtgcg attactaaat aaatatttcc gtttttcatt tgtttttttg
    19321 aagaattcat aagtcacctc tgtattttat cttagacttt ttttgaagaa acaacaatca
    19381 ttttcataga gacttcttcc ataaaaaagt gctatactta attttgtatt acaatatgaa
    19441 gggagttttt tcatgagtta ttcattaaat tggaatctag attctatttt ttccggaggc
    19501 agtcattctg atgctttgaa ccaacggatg aagcaattgg aagaccaaat gaacgagtat
    19561 tatcaccgcg taacaaaatg gtcacctagt tctgacaatg cagagcagtt aaacgctatt
    19621 cttcaattgc aagaaacgat tacgaatggt tttacccaat gcagcagcta tattactgca
    19681 ttgctttctg ccaatgtcaa tgattcagat gcaaaggtct tatcaggcaa gctttacgca
    19741 atgctgcctc gtctgcaatc agccgaaact gtattgtcaa agaaatttgc agaaatatct
    19801 gacaatgatt ggaatcagat gctttctcaa tcttcgtttg aaacaattgc ttttcgttta
    19861 aacgagattc gtcgtgatgg ctcacaatta ttatcagaag ctgaagaaaa cattataaat
    19921 acgctttctt tagatggtct aaatgcttgg agcagtcatt atgatacgat tgtagccagc
    19981 atttcgattc ccttcgaaca agatggacaa gtagtcgaat tatctgctgg acaagccttc
    20041 aataagatga tgggcgatcc agatccaaaa gtcagagaaa cacttttcgc agcatgggaa
    20101 gaagcctgga aagaaaaggc acccctcttc acggatactt tgaatcattt agatggtttt
    20161 agactttctg actatgaact tcatggtgtc acagattttc tacaaaaacc attagagtac
    20221 aatcggttga agaaagaaac attgtctgtc atgtgggata caatccagaa aaacaaacaa
    20281 ccccttgtta attacttgac tagaaaagct aatcttttcg gtaaagaaaa aatggaatgg
    20341 caagatcaag atgcacctat tattttaggc gatttgaaag aaaaaacctt tagttttgac
    20401 gaagcagcag catttattat cgaaaacttt aacaaattca gtccaaaaat ggctaaattt
    20461 gcccaatctg cttttgaaaa gagctggatc gaagcagaag atcgtcctgg gaaacgtcct
    20521 ggtggttatt gtactgagtt accagaaaca aaagaatcaa ggatctttat gacctacagt
    20581 aattctgtta atgaggttgc gacacttgct catgaactag gacatgcttt ccatagcagc
    20641 acgatgtggg atttaccttc tttaaatcgc gaatatgcca tgaatgtcgc agaaacagca
    20701 agtacattcg cagaactgat cgtagccgat gctacattaa aagtcgcaaa aacaaaagaa
    20761 gaaaaaatca atttgctaga tacaaaactg caaaatgctt tagctatgtt catgaatatc
    20821 cattcccgat tcatttttga aaatcgattc tatgaagcac ggcaagaagg attagtctca
    20881 gaagataaga tcacagagat gatggttgag gcgcaaaaag aaggctatca aggagcttta
    20941 gctacttacc atccatattt ctgggctgca aaattgcatt tctttattga tgacgtaccg
    21001 ttttataact tcccatacac atttggctac ctctttagtt tgggcatcta tgcttacgcc
    21061 aaccaaaagg gagcaaattt tgaagaagaa tatatccatt tactacgcga tactgcctca
    21121 atgacgacgg aagatcttgc gaagaaacat ttgaatgtcg atcttaccca accagatttt
    21181 tggcaagcag gtatcgacat ggtgactgaa gatatccaaa cgttcatgga attgacagaa
    21241 gaatttatct gatttctatt ttatcacaat aaaaaaagag ttagtccaga atgggctaac
    21301 tctttttgat taatcattct tacatgctga cagcaaagtc aggggcaaac cattgaactg
    21361 aaccaacttt tacgctttcg cctggttgag gagcatggat atattgtcct ccgcctaaag
    21421 caatcgctac gtggtaagtt ccgccttgtg acccccagaa taacaagtct ccagcttttg
    21481 cttgagaaac tgaaattttt gttccagctg attcttgagg aactgtccaa ccaccgatgt
    21541 cacgaccagt tacttgcatg taaacatagc gtgtgaaccc tgagcagtca aagccacttg
    21601 gatctttacc gccccaaaca tatggagtac caatatattt gtaagcttct gctacgattg
    21661 ctgcaccatt cacacttcca gaaggagctg gagcaggtgt tacaggtttt ggttgttcag
    21721 gtgttggtgt tggcgttgga gccggagtac tacttcctgt gccgtttcct gtatcaacac
    21781 tttgatctgt tgatggtgtt gtaggcgcag gtgttgttga ttcttctgtt gttgaagatt
    21841 caggcactgt tgttgattct tctgttgctg aagattcagg cactgttgtt gattcttctg
    21901 ttgctgaaga ttcaggcgct gttgttgatt cttctgttgc tgaagattca ggcgctgttg
    21961 attcttctgt tgaagattca ggtgttgttg attcttctgt tgctgaagat tgtgtagctg
    22021 atgatgattc ttcagtcatt gaagattgtg tagctgatga ttcttctgtt gctgagcttt
    22081 gtgcagtaga tgaagcttga gtagcttgtg cttcagcctc tgcttgttcg cgtgcttctt
    22141 tttcagcttt ttcttgtgca gcttgttgac gagcttgttc tgctagacgt tgttgttcac
    22201 gaatacgagc ttgctctgct tcagcttctg ctttttgacg gttcaagtca gctttcttgt
    22261 cttcagcagt tgcttgttca gcagctaatg aagttttcag tacgttcaaa tcagcttgtt
    22321 tagataaaag atcgcctttt tgagattcca aagcagcttg gttttcagct aattctttta
    22381 gtttagcatc gttttcagct tttttgtctt ctacagcttt tttatcttgt ttttgttgtt
    22441 ccatcaagtc gttgtttgct tttaccatcg ttgtcattgc ttgaacgcgt ccaatagcat
    22501 ctgctaatga atcagcattc aatacagcat cgatgtagtt tgagcttgtg ttgcttactt
    22561 gtgcttcgcg tgcttgtttt tggattgtat cttcacgttt ttcaatacgt tcttgtaaat
    22621 ctgcaatgtc ttttactaat tgtgcagatt cttgacgcaa tgtatcttgt tttgctaata
    22681 ggtcttgtgc ttgtgtgttg atttcagaaa cttggctttc taaggcatcg atttgtgatt
    22741 gtgcatctgc ttgttgattt ttcaagtctg caatcttttg atcttgctgt tgtatctgag
    22801 aatcaaaatc gtccgccgcg gcagcgatcg gtgatgctac ggcagtcaag gtcattgaac
    22861 ataccattac tgctgatatt aaactctttt tcactcttca ttcctccgac tggcttaatt
    22921 taataaatat ctaaatatat ttaagtcttt cttaacgata taaaaattgt accatagcag
    22981 tgtgtcaacg atataatagt tatgttacaa aaatgtttca aaacaacttt taaaaatgac
    23041 aaaactgttc gtttcagaaa atgatcatga aaaagccgcg ttaagtctag catttcttaa
    23101 gataacaacg attctttttc gctttcgacc taaaaataaa aaaagaaatt attcatcact
    23161 gaataatttc ttaaagggaa aaatgaataa tacaaatata atcatgttca acagcagtgt
    23221 aggtcctaaa aaccttgtaa taaaatacgt gttatttact actgctaatt taaaaatcaa
    23281 ctggaccacc atcgtaaaca attcataacc cgtgacaaag atgatcatac tgaaaaattc
    23341 agtaaagatg ttgacatgga taacgtcttt cattacatac atcaaccata caattaaagg
    23401 gagagctact gcatagatac cgataacgcc aatatagtaa gcgtcataga tagccccgag
    23461 aacaatcgtg gtaatcagta aataacgttt ttcaaacgcc atactgcaac ataataatgc
    23521 caatatcaga aagtgggcat ttgacatata ggtgcctttc gaccactccc ctagcattct
    23581 tgttaaatga ccatctatca acatcaaaaa gaacaaaacg attggcagat agtacttcat
    23641 tgtttctttc ttcaacatgg actaatcctc cgctagccgt tggacaatcg tcacaactga
    23701 cacgtcatac atttctgcat aaggtttcac ataaacttcc cgatccagcc cataactatc
    23761 tggtttcgtt ttaataaccg taccgatcgg caagttagct ggagaattcc ctccaagacc
    23821 ggatgtctgg acaacatccc cttctttgat atccgtatca ccagttaatt gggtcaccac
    23881 taaggcatgg agcttttcat catagttttt aagcaaacca aacgcttcgc cattagccga
    23941 agatacccgt actggaaaat gattggagct ttcattagag gatgacagta attcgatttt
    24001 agacgaagcc gtattgacct cgatcactcg gccaatcaat cctttttgcg acatgactgc
    24061 catatttgct tcgataccgt ctttagaacc cttgtctacg atcagcatgt cctgccaact
    24121 gtcaggcgaa cgagtaatga catttgccgt tactttttcg taactagtca gcgtctgatt
    24181 caactcaagt tcttttttca acgcttcgat ttctctctcg taattcttgt tttgctgtga
    24241 aacgtcatca tagctatcga ttttttcttt tagccgctca ttttcggcat aagttgaaaa
    24301 aagattatgg acagaatcta ctccatttcg tacccaccgt actggggcag agattgtttt
    24361 atcaaccatt gcaacactgt cattaacgat ggattggaag aaattgggct ttccttcatt
    24421 cgctcgctgc gcagcggtca cacttagtat cgtcaccacg atgataacaa taaccaaggt
    24481 aataataata tttttattag gattgaactt cttcacaaag acacctcgaa ttaattagac
    24541 tcttgtgttc aattttatca ctaaatgccg tgaaacaaaa ggtaagttgc tcctcttaag
    24601 ctttttttta gcaaataata gattctgtca tttttagcct gaaagacaaa tttttttatt
    24661 tgtttatgga tcaatctttg ctttgaataa ctgcaatcat accatctgaa aaagaaaatt
    24721 ctgcggtatc tgaaagaaat tcgttacttg cataagatgt cggatattct acttctttat
    24781 tttccaatac atacttgctg ccttttaatg taagattcga gacgggagtc aagcagcaat
    24841 aagctaaata cttcatatct ggtatctgtc ttactatatg ctctccaggc aaataataag
    24901 acaaataatt ttgacaatct ctgataataa gctgatgggc aaagggtttg aagcgaggtt
    24961 ccatacctaa ccagagattg gctaaaagat gatccaatct tccgcccgta gctccaatca
    25021 aagtgacttc cgcttgcggg taacgttcaa atgtttttaa gatacctaac tgtgtatctg
    25081 tatcgtcttt ttctgcaggg gaacgggtat actctttggc ttttgcaaat acttccttct
    25141 gctcttcgtt cgacaatgaa tcaaaatcgc ccactgccag atctactggg tagcctgctt
    25201 gaagcaggta cagcgcacca cggtctatcc cgacataggc atcgtatttg gacagcagag
    25261 actgcggcca gttttgcgga ttacctccag cagctaataa aattttcatt tagtctaatg
    25321 cctcttttaa tcgttcgatt tgtccgactg gattttcggc tccatagata tatgatcctg
    25381 caacgaagac atccgcccct gcatctttac agatttttgc tgtttctgga acgattccgc
    25441 catctacttc gattgcataa gtccaattgt tctctctacg tagatcagcg agttccgtaa
    25501 ttttttctac tgtttcttca ataaagcttt gtccgccgaa cccaggattg acagtcatga
    25561 ctagtacttg atcagccaaa ggcaaaacat gtttgatcat ggagactggt gttcctggat
    25621 tgataactac tccagcttta actcctaagc tttttgtcat ttgaagtgcc cgatgaatat
    25681 gaggagttgc ttcgacgtgg acagtaatga tatctgctcc agcttttgca aatgctggaa
    25741 tgtagttttc tggatcaacg atcatcagat ggacgtctaa cggtaattta gtaacaggac
    25801 ggattgcctg aacaatattt ggccctaatg tgatatttgg gacaaattgg ccatccatta
    25861 catctacgtg gatgtaatct gcgcctcctt tttccactaa ctcaatgtct ctttgtaaat
    25921 tggcaaaatc tgcactcaaa atggatggtg ctatcttcat tcttgtttcc tcctattttc
    25981 gtttaccttc tttttttgca tacattggtt ttcttttttc gatttcttgt aagaattgca
    26041 aataattgtc gtaacgtgtc tgtgcgatac cacctgtttc tactctatgt tttacttcgc
    26101 agccaggttc tttagcatgc atgcactctc taaatttgca atggacagaa gcttctacaa
    26161 attcaggaaa ttgtctcggc agttctcctg cttcaattgt caaaaagtca atcgagctaa
    26221 atcctggtgt atctgctact aacccgccat agataggaat cagttccaca tgacgtgttg
    26281 tatgccgtcc tcttccaagt gcatcagaaa tctctcctgt tgctaaattc agttccggtg
    26341 atatttgatt cagtagcgtc gattttcctg ctccagactg acccataaag accgatagcc
    26401 gttctgggaa gtaacggatc agttctgtcg tatcacccgt atgattgggt acaatcaccg
    26461 gatagcctat tttagtatag gtttgcttga tctcttcgat tttttctggg tcgtctgaca
    26521 agtcggtctt tgtcaaaaaa ataatcggtt cgatcgactc gtattccaag gtaactagaa
    26581 agcggtcaag tagattataa gaaaatgcag gctctacgat acttgtgaca atgattccaa
    26641 gatcaacatt tgtgactggt gggcgaacta attgattttt tctcggcaag atttccagaa
    26701 gatagccgtc tgtttgattt tcactctcaa agatcacctg atctccgaca agcggcgtga
    26761 ttttccgatt ccggaaattt cctcttcctc ttgtttgata ggtctctccg tttgcgtata
    26821 catagtaaaa accgctgatt gcttttctga tttgtccttt gataagtgcc acctccgttt
    26881 ttgtttattt taccatacga agaggaaagt aggtgttatt ccatgcaaac ttctcaaagt
    26941 atatggcttg aaaattgatc aaaagagata gaaacacaaa aaagagaatg caacaaatca
    27001 atgtcgcatt cccttctgat atattttatt cagcacttat tttgaacttt ctgaggtgct
    27061 agctgatgaa cttgtggagc tggaagattc ttcttttgaa gagttgcttg tgctactgga
    27121 gctacttgag ctgctagaac tgtctgaaga gtctttttca gaactagaag acgctggatc
    27181 tggtccttta gagtaaatta cgttcacaac cattcctttg gtaattgtcg ttcctgcccc
    27241 tggtactgta cgaatcactt tatctttatc caccgtgttt gaatattcgg aagtctcatg
    27301 cccgatatat tctgcaccga cactagctaa ataactttgt gcttcgcttt ttgtatagcc
    27361 ggagatatca ttcaatttag gctctgttcc ttggcttacg acaaaattga tgtaagtttc
    27421 ttctgctaca acttcacccg aggctggatc ctgactgatg atcgaacctg atggtacagt
    27481 gtcgctatag tcataagtag cactaatatt attgatattg aatccttgtg taacaagcca
    27541 attacgaaca gtttcgtagt tttctcctac atgactactc aaagtcactg tttctggtcc
    27601 tttgctgact gtgaaagtaa tcgtgtctgt tttaggatcg acttctgtat cttctgatgg
    27661 agattgcgaa atgatcattc cgctttctac ctctgaactg aattcctctt ctttttgaat
    27721 caagtcagca gaaaacccta attctttcaa acttgcttcg gcatcttcat aacttttacc
    27781 actgtaatca tctaatttga tttttttcgt gccgctacta atatacagag tgacttcgcg
    27841 tttttgcttg actgtcgttc ccgctgccgg gtctgtcttc actacattac cttcttcgat
    27901 tttgtcatct gcaatttctt ctgtttttgt tgctacttct aatttcgctt ttgcgagtgt
    27961 ttctctagca gaagcttcag aaagatcaga aacatcagga acaacaactt ctcctcgccc
    28021 gccagaagca aagaatacag ctgttccgat tcctaaaaca gcaagaatag ctaataggat
    28081 cagccaccat tttttctgtc ctttcttttt aggatcaggc agaacttctt cctcctctgg
    28141 caaggtttcg ttttctggct tgtcttttgg cagaggcata gatttgaatg aatcaggcat
    28201 tggtgcatct tccgtaaggg gtgtgataac ttttgtttca ttatccatca catgaggctg
    28261 ccatttttct tcgtttgcac gagctggtga taatactgta gaaagatcat cagacatttc
    28321 ctcggctgac ttataacggt ctgcaggttc tttagccgtt gcccgtaaaa tcacgttttc
    28381 cagtgcttgt ggcacatttg gatcaagtgc ttttatagat ggaagatcat cttgaaaatg
    28441 ttttaaagca attgttacag cagattcacc atcaaacggt acgctacccg tcaacatctc
    28501 ataaaggatg atccctaaag cataaatatc tgactgcttc gttgccatac ttcctctggc
    28561 ctgctcaggg gacagataat gaacagatcc cagcatcgta ttcgtctgcg tgatcgatgt
    28621 ttcagataaa gcgatagcaa tcccgaaatc cgtgattttt acaacacctt cattatcgat
    28681 cagtacgttt tggggtttca aatctcggtg gatgatttga tggttatgag ctaaagaaat
    28741 agcagaaagg atctgctgca taatatttac tgctgtttca taaggaacag gataatgtgt
    28801 ttggatatat cgtttcaagt ccattccttt gacgtattcc atcaccagat actgcatgtt
    28861 gtcttcttca ccaacatcgt aaacactaac aatgtttgga tgaacgagtt ctgttgctgc
    28921 taaagcttct ctctgaaatc gtcgaatcgc agtctggtca ttttgaaagt caaatcgtaa
    28981 gactttaact gctacatcac gatccaagat taaatcgtgc gccaaaaata catttgccat
    29041 accaccgcta ccgatatttc cagtgatttg ataacggcca tttaattttt tgcccaactc
    29101 gatcatacac tgtcctcctt atattcgata agaagaacgg tgatattatc cgctccgcct
    29161 gcttcattgg cacgatcaat cagctcagtt actttatgag acaatgggcc ttcttgattg
    29221 atgatcgttc ctatttcttc ttcagacagc atgttcgtca agccatctga acataacagc
    29281 agtcgatctt ttagttgcca aagatgagta gaaacatcta cttcgactgt tccaggcatg
    29341 ccgacagaac gtgttaaaat atttttcctt ggatggttca ctgccatttc ttcgctgatt
    29401 tctcctgatt tgactagttc attgactaaa gaatgatcct ccgtcaattg catgatttta
    29461 tcttcacgaa tcaaataagc tctgctatcc ccaacattgg caatcgtgaa ccagtcatct
    29521 gacagagcgg ccgctacgat tgttgttccc ataccgttat attccggctg ttcttgtcca
    29581 cgctgataaa tcagcatatt ttcttcttga atcgattgaa tgaaccattg ggcgattttt
    29641 tcatcgtcaa cagcttcttg tttttcccaa gcttcgccta gattcgttac cgccatctga
    29701 ctggcaatgt ctccggcacg atgaccaccc atcccgtctg ctaatacagc aagcttgaga
    29761 cctgaacggc tggtaaatac actggcatag tcttgatttg tgttacgtcg tcttcctaca
    29821 tctgattgat attcaatctg cattttttca cctcattact ttttgcgcaa acaacaaatg
    29881 aaaaatccat ctgtgtaaaa ctgatgcgga tacaaagtca acattttctc ttcgattgat
    29941 gattgtagca taggattggt caaaacatca atcctctcaa aatcagggtg tctttccaag
    30001 aaagcagaaa caacgtcttg gttttcttct tttaagatcg tacaagtgct ataagtcaga
    30061 ataccggaag actttaaagt tggtgcgcag ctttctaaaa tttccaattg gatttttggt
    30121 aaattcgcta attcatccgg ctgtttttta taacgaatat ctggttttcg ccgaagcagc
    30181 ccaagacctg aacaaggcgc gtctactaaa atccgatcaa actgctctgc ttcgaactgt
    30241 ttactgactt ctctagcatc catcttttga gcgtagacag cagaagagac acctagacgt
    30301 gctgcattat cttcaatgag tttgactttg tgttcgtgta catctaaagc gaccacttct
    30361 ccaccgcatg acggatctaa aaaagaagca atatgcgtcg tcttacctcc gggtgctgca
    30421 caggcatcta agacatgatg ttcaggctcg atttgcatag acggcgctac aagcatcgaa
    30481 ctttcatctt ggatcgtcat gacgccatcg tggaacaatg agctagaagc taaatgccct
    30541 tttttcgcta ctataccgta tggactgact tggctttttt cagctgtgat ctcttcttct
    30601 tgcaaaacag tgatggcctg atctctggaa agtcggcgtg tatctactcg ggcactagca
    30661 tggctaggct gatatagcga taaaccgagc ttttcagttt cttcgtatcc gatttgatcc
    30721 accagtcgtt tcgtcatcca tagaggcagg ctgattttga cagaaagtct ttcgatagga
    30781 tcagcaatgc cttgcagatc tggtgctcct tgacgctgat aattcctcaa gacaccattt
    30841 acaaatttcc ctgtcccagg atttcctttt acttttgcga tttcgaccgc ctcgtttaaa
    30901 atagcatgtg cagggacttt atctaaaaat tctaattggt atacagaaag ataaagaagc
    30961 gtcttgaccc agtcatccac tttttttgct ttagcgatca atggtgcaac gtaaaattcc
    31021 aatagtcgct gatggctaat cgtaccatag atcaattctg taaataaacc cacatctttt
    31081 tgatttagct cgctttgttt gatcatctca ttcaacaaaa gattcgagta ggcgccaccc
    31141 ttgttcactc gctctaatgt cagtaatgcc atatatcgta ctgatttttt cattttttta
    31201 gggatttttt tcattatttc acctgctgac caatctgaac ttgctgaccg cttccgttaa
    31261 ggaaatcatg tacggcctgt tttcctttac cagctggctg taattcattg atggctaaga
    31321 cggtctgttt accgcaagca atccatagat tttttttgtc tttccggatg atcgtaccag
    31381 gtgctttgtc tgtagtttct gccaatactt caaccgataa taatttccag cgtatgccct
    31441 cataggtcgt gaatgcaatt ggccaaggac gcattccgcg tacttggttg tcaatttctt
    31501 ctgcagtttt atgccaatcg attgcttctt gttcccgtgt gatgtttggc gaaaaagttg
    31561 cttctgactc gtcttgaggt actggttttt gtccatccaa aatattgggc aatgtttcta
    31621 gcaatagctt tcttcctaaa aggctaagct tttcaaacat cgtccctaca tcatcttgtt
    31681 tagtgattgg tatgctttct tgcgcataga ttccgcctgc atccattttt ttgatcattt
    31741 ccatgatcgt tacaccagtt tctttctccc cattcatgat ggcataatga acaggcgcac
    31801 ctccccgata tttaggaagt aaagaagcat ggacattgat tgcccctaat tttggtactt
    31861 gcaataactt ttcaggcaag aattggccaa atgccgcagt gacgatcaca tcaggcacca
    31921 attcaatgat ttcttccatt tctggcgaac cggagatttt ctctggctgc agcacacgga
    31981 taccgtgctt cagagccgct tcttttaccg gtgtcggcgt aattactcgt ttccgcccaa
    32041 ctggacggtc gggctgtgtt accactgcct ggatctcgta acctgattct atcagccctt
    32101 caagaatcgg cacagaaaaa gcaggtgtgc ccataaatac tagcttagtc atcaagatgt
    32161 tcctccatat attcttcaag ttcttcttcc gggatctgct gtatcatttt ctcaatgaac
    32221 aagataccgt ctaagtgatc gatttcatgc tggaaggcac gggctaaata gccaaaagcc
    32281 gtcacttcta tctcttctcc gtctcgatca taataacgca cggtcacttc atctgctcgc
    32341 ttgaccgttc catagacatg cggaatactg agacaacctt ctacgtcaag gctttcacct
    32401 tttgcttcga tgatttctgg gttaatcagt tcaaatttct cgccttcatc tacttcgatc
    32461 acagcaattc tcttgttttg tcctacttga ggagcagcga tccctattcc gtcattcgca
    32521 atcattgtct catataaatt gtctaataaa gcgattgtct cgtccgtgat tacatcgatt
    32581 ggctgagcag ttctttttaa tttatcatta gggtggatca atataggata gcgcataatt
    32641 tggccctttc ttaaataaag tttaatggtt cgttgtcaat tgccacaaaa agaccttgtc
    32701 gctgttcttt ctggcttcca ttcaaaattt catccaaaac atgaggaaga ttcggttcat
    32761 gtttatattt gatgattagt tgataataat atcggttttt cactctcaaa atcatgctgg
    32821 gcgttggccc taatagcaag ctttgagggc ttaacacttc ttttagctga ttggcaatct
    32881 gaaagatttt ctttgccgct acttgttctt caggatggct ggcggttatt ttcacagtaa
    32941 agtaataagg aggatagcct ccacgatgcc gtacatgcat ttcctgctga taaaactgct
    33001 cataattttg ttttttagct aattctatgg cgtaatgttg aggattgaat gtttggatca
    33061 cgacttcgcc tgctttttcc gctcgccctg ctcgtcctga aacttgggta agcaattgga
    33121 atgtatgctc actagaacga aaatccggta aattcaggga ggtatcagca ttcaacaccc
    33181 caactaatgt cacttctgga aagtccaaac ctttagcaat catttgtgtc cctaataata
    33241 tatctgcttc ttttgcaccg aatttttgta aaatctgttc gtgtgcccct tttcttcgag
    33301 tggtatctac atccatacgc aaaatcctag cttgcggata aagttctcgg agttcctctt
    33361 ctactttttg agtacctgtt ccatagtagc ggattttatt gccgccacaa ttaggacagc
    33421 gattgggaat aggttcttca tgtccgcaat aatggcagcg cattgaacgt gtatccatat
    33481 gcagtgtaag agagatatcg caattaggac atggaagaac aaatccacag tccctacaca
    33541 tcacaaaaga cgaatatcct cgacgattga gcagtaggac agtttgttct tttttgtcta
    33601 atcgattttg tattttttcc tgcaaattag cagaaaaagt agaggtccga tgattctgaa
    33661 actcttcacg catatctacg atctcaatag ctggaagctg tgcagctgct ttggctcggt
    33721 ggtttaactg caataactga taaacgcctt tttgagctcg tgctcttgat tctaaagagg
    33781 gcgtcgcgct tcctagaact attgggcaat gatgatattt actgcgccaa attgctagat
    33841 ctctcgcatg ataacgcggt gtctcatctt gtttgtaact tgattcatgt tcttcatcga
    33901 taatgatcag cccgatattt ttcagcgggg caaaaatagc agatcgtgcg cctactacta
    33961 cttgcgcttc ttcccgctca atcttgcgcc attcatcata tttttcacct tgggaaagtc
    34021 cgctatgtaa gaccgcaact gattctccga agcgtccttt gaatcgctcg accatttgcg
    34081 gagtcaatgc gatttcaggc actaacataa tagccgtttt attttctgac aaaacatcag
    34141 cgattgcctg aagatatact tctgttttcc cgctgccagt gattccctcc agcaaaaaga
    34201 cctgatcttt ttgctgctga cctgctgtga tgatttgttc gacagcattt ttttgttctg
    34261 cattcagttc caatgccgtc gtttggtcaa atactcggtc tttataaggg tcgcgataac
    34321 gttctgattc tataaactcc agccagccct tttcttttcc ttgattcaag acagcagtac
    34381 tgattccttt atttttgaaa taagcaattg gttcttcttg gctcagttca gataaacaca
    34441 aaagaagttc tttttgtttc ttggcatttt gccgtaattt tgcccattct tcttcgattt
    34501 gcttttcagt taacaaagaa cggaccaaac gaatcgtttt tactttattt cgtgtatgga
    34561 cttcatattt gatatctact aattgctctt ggcgccagcg aatcaattga gaaaggctgc
    34621 cttcttcttc tgcttccttc caggaaatca cttctcgctg atcaaacaat acctgaactt
    34681 ctggtgtgtc tgccaaagga tagatcaatt tatcatattc tgctttcatc acactcggaa
    34741 gcatcgtttg tagacaagtg attttgaatg cgtaagtaat ttttttcata taatcagcta
    34801 atgaaagtaa ttcttcattg accaccggag caaggtctag caaacgaata actgccttca
    34861 aagatggatt agctgattct tctgattttt ggatagccat aacaaatcct tggacatgac
    34921 gattgccatt accaaaaggg acttctactc gcatcccgac ttgcaaagct gtttccattt
    34981 cagagggaac caaataagtg aatggctgat cggtctgcat agtaggtacg tccacaatga
    35041 cttcagctgt taatgtcatg gttcgtttcc tccttttttt gtcttttctt atcaagcgac
    35101 aaaaagttcc ttgcctattt tactagagat cgatggattt gtcttatctt ctgccaaatc
    35161 ctaacaaaaa attaacagca gataagaaaa aaaagaactg tgtcaaaaag ttcagacaca
    35221 gccttttcta ccagacaata agtggcgaat gtttgaaaag gaaacagctg ggtttgcttt
    35281 tctttttaga tgttcgctct atttttcttt attacatttt ttgctcttca cggatacgtg
    35341 attccaattc gtgttgttca cgctcttttt gtgcttgacg ttcctcttct tccatcttca
    35401 aacgttcacg ttttaattca ggatgtggat cattgactac atttcctgca tcgatttctt
    35461 ctaaagcttg accgacgctt tttactgaat caaaagaatc tagagtaggc tgtgcacttg
    35521 catccaattc atgcgcgcgt ttacttgcta aaatcactag agaatatttt gaatttacac
    35581 gatctaatag tgagtcaatt gaaggtttta acatcataag actacatctc ctttaacatt
    35641 ttaatatatt taccgattac gcgatctact cgaaaatgtt cactggcaat aatgtttttg
    35701 attcgttcaa ctgctagcgg cacttcatca tttacaacag catagtcgta aagagccatc
    35761 atttcgattt cttcttttgc aactttcatt cgttcttcga ttacttcatc tgcatctgtt
    35821 cctcggccta cgatccgtga ttttaattcc gctaaatctg gcggagtcaa aaagatgaat
    35881 acaccatcgg gcactttttc tttgacttgt tttgctcctt ggacttcaat ctccaagaaa
    35941 acatcttttc cttcatccaa tgtcttgttg atatatgaca aaggagttcc ataataattc
    36001 ccgacatatt ctgcgtattc cagcatctct ccagcttcga tcattgattc aaactcttct
    36061 ttgcttcgga agaaataatc aacgccttcg acctcccctt cacgcatttt acgggtagtc
    36121 attgagatcg aatactgaaa atcattatcc tcactctcaa aaattgcttt tcgtactgtg
    36181 ccttttccta caccggatgg accagacaac acgattaata atccacgctc tgacatgacg
    36241 acgtcctttc aactagtctt tttgtactta tcgtttattt tgcacttttt ttctgcaact
    36301 ttcaaggtta tctttaatta tagctagaaa ttggtaaaaa agctaccctg taccaagagg
    36361 tcgtaaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacataaac agcttctcat
    36421 gtttttcgtt tggttggggt tattatcgca gatgcaagct tttgaacgcc gtgtataaga
    36481 ggtcgtgaag aagccgatta gtttcgagta tgaagaaaaa ttttgtgaaa atagtttctc
    36541 acatttttgc ataaatttgg cttaata
//
LOCUS       DASHRQ010000025        34866 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.025, whole genome shotgun sequence.
ACCESSION   DASHRQ010000025 DASHRQ010000000
VERSION     DASHRQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.025"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..106)
                     /locus_tag="U2M88_001333"
     rRNA            complement(<1..106)
                     /locus_tag="U2M88_001333"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            847..1536
                     /locus_tag="U2M88_001334"
     CDS             847..1536
                     /locus_tag="U2M88_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8373724.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1523..2731
                     /locus_tag="U2M88_001335"
     CDS             1523..2731
                     /locus_tag="U2M88_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEM8373725.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLLEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            3025..4296
                     /gene="serS"
                     /locus_tag="U2M88_001336"
     CDS             3025..4296
                     /gene="serS"
                     /locus_tag="U2M88_001336"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEM8373726.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4845..6329)
                     /gene="guaB"
                     /locus_tag="U2M88_001337"
     CDS             complement(4845..6329)
                     /gene="guaB"
                     /locus_tag="U2M88_001337"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEM8373727.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6834..7529)
                     /locus_tag="U2M88_001338"
     CDS             complement(6834..7529)
                     /locus_tag="U2M88_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEM8373728.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7619..8719)
                     /gene="ychF"
                     /locus_tag="U2M88_001339"
     CDS             complement(7619..8719)
                     /gene="ychF"
                     /locus_tag="U2M88_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEM8373729.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8728..8919)
                     /locus_tag="U2M88_001340"
     CDS             complement(8728..8919)
                     /locus_tag="U2M88_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEM8373730.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8940..9830)
                     /locus_tag="U2M88_001341"
     CDS             complement(8940..9830)
                     /locus_tag="U2M88_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEM8373731.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9817..10584)
                     /locus_tag="U2M88_001342"
     CDS             complement(9817..10584)
                     /locus_tag="U2M88_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEM8373732.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10596..11309)
                     /gene="rsmG"
                     /locus_tag="U2M88_001343"
     CDS             complement(10596..11309)
                     /gene="rsmG"
                     /locus_tag="U2M88_001343"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEM8373733.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11541..13442)
                     /gene="mnmG"
                     /locus_tag="U2M88_001344"
     CDS             complement(11541..13442)
                     /gene="mnmG"
                     /locus_tag="U2M88_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEM8373734.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13459..14856)
                     /gene="mnmE"
                     /locus_tag="U2M88_001345"
     CDS             complement(13459..14856)
                     /gene="mnmE"
                     /locus_tag="U2M88_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEM8373735.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            15155..15538
                     /locus_tag="U2M88_001346"
     CDS             15155..15538
                     /locus_tag="U2M88_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373736.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15706..16464)
                     /locus_tag="U2M88_001347"
     CDS             complement(15706..16464)
                     /locus_tag="U2M88_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEM8373737.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16482..17300)
                     /locus_tag="U2M88_001348"
     CDS             complement(16482..17300)
                     /locus_tag="U2M88_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEM8373738.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17297..17653)
                     /gene="rnpA"
                     /locus_tag="U2M88_001349"
     CDS             complement(17297..17653)
                     /gene="rnpA"
                     /locus_tag="U2M88_001349"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEM8373739.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17771..17905)
                     /gene="rpmH"
                     /locus_tag="U2M88_001350"
     CDS             complement(17771..17905)
                     /gene="rpmH"
                     /locus_tag="U2M88_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEM8373740.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18428..19762
                     /gene="dnaA"
                     /locus_tag="U2M88_001351"
     CDS             18428..19762
                     /gene="dnaA"
                     /locus_tag="U2M88_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEM8373741.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19960..21090
                     /gene="dnaN"
                     /locus_tag="U2M88_001352"
     CDS             19960..21090
                     /gene="dnaN"
                     /locus_tag="U2M88_001352"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEM8373742.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21319..21561
                     /gene="yaaA"
                     /locus_tag="U2M88_001353"
     CDS             21319..21561
                     /gene="yaaA"
                     /locus_tag="U2M88_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEM8373743.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21548..22672
                     /gene="recF"
                     /locus_tag="U2M88_001354"
     CDS             21548..22672
                     /gene="recF"
                     /locus_tag="U2M88_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEM8373744.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22669..24615
                     /gene="gyrB"
                     /locus_tag="U2M88_001355"
     CDS             22669..24615
                     /gene="gyrB"
                     /locus_tag="U2M88_001355"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEM8373745.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24635..27106
                     /gene="gyrA"
                     /locus_tag="U2M88_001356"
     CDS             24635..27106
                     /gene="gyrA"
                     /locus_tag="U2M88_001356"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEM8373746.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27342..27641
                     /locus_tag="U2M88_001357"
     CDS             27342..27641
                     /locus_tag="U2M88_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEM8373747.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27691..28203
                     /gene="ssb"
                     /locus_tag="U2M88_001358"
     CDS             27691..28203
                     /gene="ssb"
                     /locus_tag="U2M88_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEM8373748.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            28229..28465
                     /locus_tag="U2M88_001359"
     CDS             28229..28465
                     /locus_tag="U2M88_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEM8373749.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28608..30581
                     /locus_tag="U2M88_001360"
     CDS             28608..30581
                     /locus_tag="U2M88_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEM8373750.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30587..31039
                     /gene="rplI"
                     /locus_tag="U2M88_001361"
     CDS             30587..31039
                     /gene="rplI"
                     /locus_tag="U2M88_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEM8373751.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31313..32680
                     /gene="dnaB"
                     /locus_tag="U2M88_001362"
     CDS             31313..32680
                     /gene="dnaB"
                     /locus_tag="U2M88_001362"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEM8373752.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33460..34752
                     /locus_tag="U2M88_001363"
     CDS             33460..34752
                     /locus_tag="U2M88_001363"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEM8373753.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10611 a         6778 c         6893 g        10584 t
ORIGIN      
        1 tctttttccg gtggagcaag ctccggtgga aaaagaagcg ttcgacttgc atgtattagg
       61 cacgccgcca gcgttcgtcc tgagccagga tcaaactctc ataaaaagtt cgaacaatct
      121 tgcgattgtt aagctcaatt tgtttgctag catattgctt gcttgttaaa aatgtttgtt
      181 gtcttgaatt gaatcaagac gccctacaca tttggtttgt cttactttgt tcagttttca
      241 aaggtctact tcatcaggag tgtttcccga ttcgtttttg gctgccgtag caacttttat
      301 atcatatcag agagacaagt aattgtcaac tcttttttaa aagttttttc gatttcgttt
      361 ttcgaaatct tccagcaact catttatcat aacttatcgt ttgttatttg tcaagaactt
      421 ttttgaagtt tttttcgata gaatcaagct tcgcaagtgg ttctttttta acgactcagt
      481 tatcttatca tgtcatttcc aacatgtcaa caacttattt tttattattt aataagttat
      541 tggtgttctt ttgaacaaat aataatatat caacatcaca tgtgaaagtc aataagaaaa
      601 tgcttattta tcaacatttc tagcttacat gatcattggt tagttctggt cctttttcgt
      661 tcgtctttat aactccgaga gacgtaaaat ccttttgttt tcagtttaat gttcatgttc
      721 ttgtcttatt cacaccaact agatgtctca ccttctaaaa ttttccttaa caaagtgctg
      781 cttttatcta tttctgcttt tttttgctag aataatagta acaatatgac tctggaggat
      841 ttatttatga aaattttgat tgctgacgat gataaagaaa tcgttgaatt attaagtatc
      901 tatgttcaca atgaggggta tgaagcagta aaggcttatg atgggaaaga agcattatct
      961 aaaatcagaa caatccctga tattgaatta cttattttag atatcatgat gcctgagatg
     1021 gatgggatgc aagtagtgaa agagctacga aaagaatccc aaataccaat cattatgctg
     1081 acagcaaaaa ctactgatat ggataagatc aaaggactgg tcgctggtgc agatgattat
     1141 gtgaccaagc catttaatcc gttagaagta atggctagag taaagtcttt attacggcgt
     1201 acaaaaatgc agattgcccc agatcagcct gatgtattag aagtaaatgc attaatcatc
     1261 aataaagact ctcatgaagt taaaacaatc gatgggaaag aaatccagtt aacagctttg
     1321 gaattcggta tcctctactt gcttgctagt catcctaatc gagtatttag cgcggatgaa
     1381 atttttgagc gtgtttggaa acaagaaagt attgtctctg ctaaaactgt catggttcac
     1441 gttagtcatt taagagataa gattgaagaa gcaactggtg gtgaaaaagt cattcaaacc
     1501 gtttggggag tgggctataa aattgacgca agatagtaaa cgagcaactg aaaaacgccg
     1561 tcggatcaat ttgacttcaa aagaaatcag cgaattgtta gcggaaggaa tcatcacgat
     1621 tatccttttg cttctattaa atgtttctat tctggttgtt atcagttcgg tgatcaacag
     1681 ttcaccttct ttgacgaatg cgatttggga ttcaaagaat atttttgcag aacgattgaa
     1741 cactgatttg ttctggaacg gccggaattt tattattccc tttttctttc tattagatat
     1801 tggtgttttg tattggcgct tgattcgaag atatcgccaa atgcaattgc ggcatatcat
     1861 cagcgaactt cactacatcg caaacggaaa ttatgatcat cgtatccctt ttgaactgag
     1921 tggtgactta agccgagtgg taacaagtat caatggattg gtggatagta ccgtagcagc
     1981 gattgaagac gaacgtaaaa tcgaaaaatc aaaagatgaa ttgatcacga atgtcagtca
     2041 tgatatccgt accccactca cgtccatcat tggataccta ggactgatag aagatggcca
     2101 gtatcactca gaagaagatc ttttaaagta tacgcatact gcttatatca aagcaaagca
     2161 gatgaaatct ttagtggacg acttatttga atacactaag gtacgccagc ctgctgtccc
     2221 ggtgaatttt tctgcctttg acatgatcca gttgctcgaa cagttagcag ctgattttga
     2281 gttggaagct tctaaaaaaa atatccaaat acttgttcaa tcaaaagtag actcccttat
     2341 catggatggc gataccgaaa aacttgtacg tgtcttcaat aatcttttga cgaatgcgtt
     2401 gaaatacgga aaaggagcaa cgaagatcgt aatcgaagta gaacgtatcg gttcagaagt
     2461 tgttgcaaca gtcaaaaata acggagcaat gatcccacaa caggcaatcg ataatctatt
     2521 cgaccgtttt tatcgtgtag aagaatctcg ttcacaagca actggaggaa ctggacttgg
     2581 tttagcaatc gctcaaagca ttgtggcttt gcacggcgga tacatttatg caaaatcaga
     2641 taagcaatgg acttctttca tcatgcacct tccaatcaag aaaaatgaaa aattacctat
     2701 tgatacacag gaagttattg acgaaagctg aaatattcgc tattcttatt acaattatta
     2761 gaataaatga aaaacacaga acccagtaca ctttattctt tttcagagag ctgatggcag
     2821 gtgtaaatca gcaaaggata atttggaagt tccagttcgt ttacgtgccc tttcgaaata
     2881 agaaaggtcg gttcaacagc cgttatgtgt tcaacaagtg cagctgacgc tgaacttggg
     2941 tggtaccgcg aatttcagac tgattcgtcc caagaataat gggatggatc agtctttttt
     3001 tattttcaaa aaggaggaaa tcatatgtta gatgtaaaaa tgatgcgaca aaatattgaa
     3061 gaagtacaag aaaaattgat gactcggggc gtaaaaaaag aaacattagc tgaattcatg
     3121 gaactagatg aaaatcgtcg taagctacta gtcaaaagtg aagaattaaa aaaatatcga
     3181 aatgatgttt ctgctgagat cgcccaattg aagcgtgcaa aagaagatgc ttctgctaaa
     3241 atcgctgaaa tgaaagaagt cggtggcaac atcaaagcat tggatactga aattgctgaa
     3301 gtggatgaaa aattgaaaca aattgcaact actttgccaa accttcccca tgattctgtg
     3361 ccagttggaa cagatgaaga ggacaatgta gaaatccgtc gttggagcga accacgtaca
     3421 tttgcttttg aaccaaaacc acactgggaa attgcagaaa atcttgatat tttagatttt
     3481 gaacgtggcg caaaagtagc cggtagccgt tttgtctatt acaaaggatt aggtgctcga
     3541 ttagaacgtg ctgtctacaa cttcatgctt gatcagcata tttacgaaca cggctatact
     3601 gaaatgatca ctccttatat cgtcaacagt aaagcgatgt ttggtacggg acaatttcca
     3661 aaattcaaag aagatgtttt ccaattggag aatactgatc taacattaat cccaacagct
     3721 gaagtaccat taactaacta ttataatgat gaaattttag atggtgcaga tttacctatt
     3781 tatttcactg cactaagccc tgcttttcgt tctgaagccg gaagtgctgg tcgggataca
     3841 cgaggactga ttcgtcttca ccaattcaat aaagtagaaa tggtcaaatt ttcggatgta
     3901 gagcattctt acgaagaatt ggaaaaaatg acagctgatg cagaagatat cttgaaaaaa
     3961 ttgcatttgc cttatcgtgt gatggcctta tcaactggag acatgggctt ttctgcagct
     4021 aaaacttatg acttagaagt atggatacct gcacaagata cttatcgtga aatcagttct
     4081 tgttctaact gtgaagattt ccaagcacgc cgcgcaatga tccgttatcg cgataatgat
     4141 ggaaaaatcc actatactca tactctgaat ggttcaggac ttgcagttgg ccgtacagta
     4201 gctgccattt tggaaaacta ccaaaatgaa gatggttctg tcacagttcc agaagcgctc
     4261 gttccttata tggggaatct gaaagttatt aaataaagag gctgtgaaaa agccgcttag
     4321 tttcgagtat taagaaaaat tttgtgaaaa tggctttcca catttttgca taaattggac
     4381 ttaataccga gaaactggct tttgaacacc atttatcgag aggttgtgaa atcagcgaat
     4441 taacctgagt attagagaaa aaaatggaaa aatagtccct catattttac cgtttgttga
     4501 actaataccg agggtcagct gattgaacac cgtttagtgg agaggtcgta agcctacccc
     4561 ccagctccta gttacaagga caaattcaaa aaacagctct tcatatttta tgaatgttgt
     4621 acttgtgatt cggaggtggc aagggaacac tgtttattca aaaagaggct gggacagaag
     4681 cgtttaactc caagaaataa gaagaaattc acgaaaattg cttttcaaat ttttgtgaat
     4741 ttcttcttat ttccgaagga gttgcttcta cttccgccgt ttatacgtgt ttagagcgtg
     4801 agacaaaagt gttctttact tttgtcccac gctctttaat gtttttaaga ttcaactgaa
     4861 tagtttggtg cttcttttgt gatttgtaca tcatgagggt gtgattcttt taatccactg
     4921 ccgctcattt ggataaattg cgcttcatca cgtaattgct gtagattggc tgccccaaca
     4981 taacccattc ctgaacgcaa accgccgatc atttggaaaa taatatctgc aacacttcct
     5041 ttataagcaa cccgaccttc gattccttct ggtactaatt tatttgcttc attgacacta
     5101 ccttggaagt aacggtcttt tgaacctttt tccatcgcgc ctaatgagcc catacctcgg
     5161 tatgttttga atcgacgtcc ttggaagatt tcgaattcac ctggtgattc atctgtccct
     5221 gccaacatac ttcctagcat cactgcatgt ccgccggcag ctaatgcttt gacgatgtca
     5281 ccagaatatt tgatccctcc gtctgcaata atcgctttgc catattgacg agcaactgat
     5341 gctgcatcat aaatagctgt gagctgaggt acacctacac cggctaccac acgtgtcgta
     5401 caaatcgatc ctggtccgat accaactttt acgacatcta cacctgcatc atataaagct
     5461 tttgtcgctt cagccgtcgc aacgtttcct gcaatcaatg ttgcatctgc aaaagttgaa
     5521 cggatttctt gaatttttcg gatcacacct gcactatgtc catgagctgt atcgatgatg
     5581 attgcatctg cccctgcttc taataaagca ttcgcacgct caaatgtatc gcttgttaca
     5641 ccaacggctg ctgctacaag caatcgacca tgttcatctt ttgctgcatt tggaaattcg
     5701 atcactttct cgatgtcttt gatcgtgatc aatccgctta aacgaccttc gttatctacg
     5761 attggtaatt tttcgatttt atgtttttgc aaaatctttt ctgcatcttt tagagaggta
     5821 cctactgggg cagtcactaa gttgtctttt gtcatcacat cagcaatcgg catgtgataa
     5881 tctgttacaa aacgcatgtc gcggtttgta attatcccta ccaattttct gttttcaagt
     5941 gtttcaacga ttggcacacc actgatacga tatttgctca tcaattcttc tgcgtctgct
     6001 acaagatgct gtggcgttaa gaagaacgga tcaatgatta cgccactttc agaacgtttg
     6061 acttttctta cttcatccgc ttgctggctg atcgtcatgt ttttatggat cacacctagt
     6121 ccgccttgtc ttgccatagc aatggccatc ttgctgtcgg taacagtatc catacttgca
     6181 ctgatgattg gaatattcaa cttaatattt ttagctaatt gtacgctcat atccacttcg
     6241 tttggtaaca cgtgactttc tgctggtatc aataagacat catcaaaagt aaatcctttt
     6301 ttcgtgaact ttgtttccca gttggacatt tttagtacca ctcctctaat ttagtttatc
     6361 tattaaaata attgattttt tcatttaagt caatgatcta cttggaatca agttagcttt
     6421 ctcatagaat accaatataa agcgtttaca tctgtgtata ttaaaagaga ttgtaaaaaa
     6481 gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac agcttctcat gtttttgaat
     6541 tggttgggtt tattaccgca gatgcaaact tttgaacacc gtttagtgga gaggctgtga
     6601 aaaagctgct tagtttcgag tagtaagaaa aattttgtga aaatggcttt ccacattttt
     6661 gcataaattg gacttaatac cgagaaactg gcttttgaac accgtttatc gtaaaaaggg
     6721 actgtgacaa gacttttgtc acagtccctt actattattt aactatggat aaacaaaatg
     6781 aggttcttta ctacccctag atatctagca aaatagctga tctttttgct agtttatctt
     6841 cttgggacgg ataaacttcc aataatcccg tattttttct tcaagtagta acggattgct
     6901 aaagcagttg ccccgataat gataactact actggatcta atactggatt gataactggt
     6961 ggcaatagtg ttgtcgcaga aaacagtacg acccaaagaa gtgtcgttcc acctaggata
     7021 agcaatgtct tacccattcc tggtttcttg cttcgatctg ctccgggata ttcatattga
     7081 tagatgtatt tgtacatcat ataaaagacc cagccaccga ttgcagaacc tagaacgagt
     7141 gtagaaaagc cataagctgg cgttgttcca cgtgaaaaca atccggtaat cgcaatcatg
     7201 atacttaata gaccaagcaa cagcaacgta ttatctaacc acatcaataa aggggtggat
     7261 gaatttttta cttctgctgg cttattgatg attgcttccg ttctttcaga gactgtccca
     7321 aacaattgac gggctgttct tccgcctttt tgttctgcaa ctaaagtagg cagaatgtca
     7381 tgaagggctt ttactttttc ttcttcagac aaattcgctg cttctaaaga ttttttcaaa
     7441 tcaaaaatgt actgttgatt tcttttggtt aattttgttt ctaaactatt gttttctttt
     7501 acgattgctc gtaattgttc tggttccatt atctaatagt cctccttagc cgttcctttt
     7561 ctcaacggtc gtcacaaaag acattctacc atattctaca gctagtttga tagccctttt
     7621 acacattaaa gcggaataac attacgtcgc cgtcttgtac gacataatct tttccttcta
     7681 atcgaacacg tccagcttct tttgctgcat gcatgtttcc gtaatgatcc aagtcttcaa
     7741 aagagacagt ttctgcacgg ataaatccgc gttcgaaatc tgaatggatg attcctgctg
     7801 cttgtggtgc tttgattcct tttttgaatg tccaagcacg tacttcttgt tccccagcag
     7861 taaagtaagt agctaagcct aacaaatcat aagcagcacg gatcaactga tccaaacctg
     7921 attcttcgat tcctaaagct tctaaaaatt ccgctttgtc ttcttcatct aattctgcga
     7981 tttcttcttc tgcacgagca ctgaccacga tcacttctgc attttcttct gcagcaaagt
     8041 tgcgcacttc ttgtacataa tgattcgcgt ctgcatctgc tacgtcatct tctgagacat
     8101 tggctacata aagaattggt tttgtcgtca ataaaaatag tgatttaacg attttttgtt
     8161 cttcttcagt aaattcgatc gttcgggcag attttccttc ttccagtaca ggtttgattt
     8221 tatccagtac agctagttct gccactgcat ctttatcttt ggttttagca acttttgcta
     8281 cacgagtata gcgtttgttg atagattcta agtctgctaa cactagttcc aagttgatcg
     8341 tatcaatatc ttctagtgga tcaacacgtc cttctacgtg agtaatatta tcatcatcaa
     8401 aacagcgaac aacgtgacaa atagcgtcta cttggcggat atggcttaag aattgatttc
     8461 ccaaaccttc tcctttactt gcgcctttta cgattccagc aatatctgta aattcaaaag
     8521 tcgtcggaac agtttttttc ggatgtacaa gttctgttaa gcgttgcaaa cgccagtcag
     8581 gtacttctac catccctacg ttggggtcga tcgtcgcaaa gggatagttt gctgcttctg
     8641 ctcctgcttt tgtgatcgca ttaaacaaag ttgattttcc tacgttgggc aacccaacaa
     8701 ttccagcagt caatgccata aatttattca ctctttctct gcattttttt caattacttt
     8761 ttttaatttc ttttcaaaat ctcgacgggt catcatgacg atatgtccgc aattcataca
     8821 tctgattttg atatctgcgc ccattcgaat gacttcccag cgattcgtct ggcaagcgtg
     8881 aggttttttc atttcaacga tatcgtgcaa gtcgtacata aaaatagcct cctttttgtt
     8941 tattcttcat caaattgaat attcaagata tcaagaattc gagtcaaatc tttttgagaa
     9001 agatattcaa tctcgatctt acctttccca tttttttctt ggatagctac acttgtacca
     9061 aatttatcca tcaaacgatc ttcgctttct cggatataat atggtttttc ctttgctagc
     9121 cttgggattt ttttcttttc ttttccatca ttttgattta actcagaaac aagctgttcc
     9181 agttggcgga cagttaaatt ttcagttact gcacgattgg ctaatttcaa gatcaatgtt
     9241 ttatttttta aacctaacag cgtacgggct tgtcccatag ataaacgctg atcttgtacc
     9301 atttccttga caagatccgg taaagaaagc aagcgcaaat aattggcgat ataaggtctg
     9361 ctctttccta aacgactagc tacttctgct tgtgttagtt tcaggttttt catcagcatc
     9421 tcatatgctt ctgcttcttc taacggattc agatcttctc gctgcaagtt ttccagcact
     9481 gctacttgca tcatcgcttc ttcgtcaaag tctctaacga tagcagggat cgtttctttt
     9541 tcagctagtt tggacgctcg aaaacgtcgc tcccctgcaa taatctcata tccttttact
     9601 gcggaacggc gtacgatgat gggttgaaag acacctgatt gttcaataga attagctagt
     9661 tcctgcaaag atacttcttc aaatgtttta cggggctgat acggatttgg tcgcagctca
     9721 ttcaatggta gctggacaac ggtatcatct tttacatcca ccgtctctaa gttttctaaa
     9781 tcttgaaaca gtgcatcgat tcctcggcct aatcctttac ttttcttcac gacttaccac
     9841 ttcctttgcc aatgcttggt agacttcagc tcctcgagaa cgtggatcgt agtcgatgat
     9901 cggttttcca tgactaggtg cttctgataa acgaatattt cttggaatga tcgtttcgta
     9961 aactttttcg cggaagtatt tacgtacttc ttctacgact tcatttccca gatttgttcg
    10021 tgcatcatac attgtcagta agactccctc gatctctaat tctggattaa agtgtttttg
    10081 gactaaacga accgtattca gtaattggct taatccttcc aatgcgtaat actcacactg
    10141 aacaggtatc aaaatcgaat cacttgctgt aaatgaattg attgtcagat ggccaagaga
    10201 aggcgggcaa tcgatcaaaa tataatcata ctgactgctt acttctgcta aagatccttt
    10261 taatcttgat tctcttgcca tcattgacgt caattcgatc tctgctcctg ctaattggag
    10321 agtcgcaggc acaatactca aattttcgtg ttcagtgatc aaagttgcct catcaattgg
    10381 caattcattg actagaacat catagatgtc tcttgtcaca tcaggttttc tgatcccgac
    10441 accactggta gcatttcctt gtgcatccat atccactagc aatacttttt tgccaaggct
    10501 agcaagacaa gcacctagat tcaccgtcgt cgttgttttg ccgacgccac ctttttggtt
    10561 cgcaacagat attatttgtg ccatctcttc tcctcctatt tgattggctg cttattaggc
    10621 agtccgggct ttcttgggta tttctttggg gttggtttct ttttttgaat aacgatgata
    10681 tgccgttgat cagagacatt cggcaaggta accgcctctt ctttgaggaa tttaccacct
    10741 aataaagcaa tcgcttgttt tgcttctgtc aattcctctt cactttttgc tgctttcaat
    10801 gcataaaaat agccttcttg tttagccaag ggcaaacaca attccgctaa cacattcaaa
    10861 cgagcaaccg cacgagcagt gacaaggtca aatgatgcac gaaagtgagg attttgccca
    10921 aatgtttctg cccggtcatg atataaggaa gcatcgattc ctaataatcg ggttaactca
    10981 gataagaagg ttattcgttt attcagagaa tcaacgatcg ttatctgcag ttctggaaag
    11041 gcaattttta aaggtatgct aggaaaccct gcccccgaac caacatcgca taaagataaa
    11101 ggttgcgtaa agtctacttc aaatgctaat gtaagtgaat cgtaaaaatg cttcagatat
    11161 acgtcttttt catccgtaat agctgtaagg ttcatctttt cgttccattc gactaatgtc
    11221 cgataataaa tagcgaattg attcatttga tgatcggtaa gctctatccc tttttctgcc
    11281 aaggattggc gaaattcttc tggtatcata aatattcctt tcattgtgaa acagcaattg
    11341 tttcacagat tctctcccta ctttaacgca aaatctctag acatgcaatc cttccatatg
    11401 gattcttaaa aatttgaaaa gtaagaaaag ccgatagact ttttctttct agtatgactc
    11461 ataaaatata cacagaagag agaactagct ttgagcaatt tctgcaaaaa ctagttctct
    11521 cttctattta ttattacgta ttacgctggt tcactgactt tcgcaatctt cccttgttcg
    11581 atatagacca ttaggatact taggtctgca gggtttacgc cactgattcg gctagcttgg
    11641 gcaattgttt ctggttggat tttctgcaat ttttgttttg cttctgttgc taatccgttg
    11701 attgctgcgt agtcgatgtt ttctggaatc tttttcgctt ccatgcgttt taatttttct
    11761 actttttcca tggctttttt gatgtagcct tcatatttga tttggatttc tacttgttcg
    11821 atttcttttc gtgtcaattc ttcattttct ggaataaacc gtagaagttc tgcataagta
    11881 atctcaggac gtcttaaaaa gtcgcttgct agtacaccat cttttaatgg tgccgcatta
    11941 atcgttgcta aaaagtcttg gacttcttct gttggtttga ttcgtacatt catcaaacgt
    12001 ttgatttctt gttcaactgc ttcttttttc ttcaaataat tcttgtattg atcttctttt
    12061 actaagccta cacggtgacc catttctgtt aatcgcaaat ctgcattatc atgacgtaaa
    12121 agcagacggt attcagcacg tgaagtcagc aaacgataag gttcgttcgt tccttttgtt
    12181 accaaatcat cgatcatgac accgatgtaa ccgtcacttc gtttgatcac taaaggttct
    12241 tttccctgaa ctttcaaagc tgcattgata ccggcaatca gcccttgtcc agccgcttct
    12301 tcataaccgc ttgtaccatt tgtttgtcca gctgtataaa gattttcaac gactttagtt
    12361 tctaatgtcg gacggagctg gtgtggctct actacgtcat attcgatagc atatcctgta
    12421 cgcatcattt ctactttttc taatccagca atagaatgaa gcatgttgac ttgaacatcc
    12481 tctggtaaag aagtagataa accttgaaca tagacttctt ccgtatttaa gccttctggt
    12541 tccaaaaata attgatgacg tggtttgtct gcaaaacgca cgatcttatc ttcgatggat
    12601 ggacaatagc gagcgcctac cccttcaacg atccctgtaa acattggggc acgatgtaag
    12661 ttgtctcgaa taatttggtg agtcgtctca cctgtatagg tcagccagca agattcctga
    12721 tctagtttat aggcactatc tggtgtgctg aagctaaaat gattcggctc tttgtcacca
    12781 ggctgctctt ctgtaacaga atagtcgatc gtacttgatt ttactcgagg cggtgttcct
    12841 gttttgaacc gttcgatagc aaagccaagt tcttttagat gattcgctag tccaatagaa
    12901 ggttgagagt tattcggacc tgatgaatat ttcaattcac cgataatgat ttctccgcga
    12961 agtgctgttc cggctgtaat aacaactgct ttggcccggt agctcgctcc tgtggacgtc
    13021 actactccat gacaaacacc atcttcgacg atcagttctt cgacgattcc ttgtcttagc
    13081 gtcaaatttg gttctttttc gatcgtatgt ttcatttctg ttgaataagc atgtttgtct
    13141 gcttgtgctc gcaatgctcg gacagctggg ccttttcctg tgttcaacat acgcatttgg
    13201 atataggttt tatcgatatt cttacccatt tctccgccta acgcatcgat ttctcgaacg
    13261 acaacgcctt tagctggtcc gcctacagaa ggattacatg gcatgaatgc taccatgtcc
    13321 aaatttattg tcagtaataa tgtttttgat cccatacgtg cagcagcaag tgcagcttct
    13381 gaaccggcgt gtcctgcgcc aacaacgatc acgtcatacg catctgcttc ataatgttcc
    13441 aatcgtttgt tcctcctttt attttcctaa acaaaattga ctgaataatt gagtgattag
    13501 ctcatcttgt acactgtcgc cgacaacttc tcctagatag tcccagcaac gcgtcatatc
    13561 tatttgaacg agatccacag gcattcctga atcaatacct gcaatgactt cttcaagtga
    13621 gagggaagct ttttctaaca acgcgatatg gcgggtatta gaaacataag tagcgtctct
    13681 ttctcctgtt tctccgctga agaataaatc agaaatagct gattccaaac gatctagtcc
    13741 gtcgttttta gctacggaaa ttgaaaaaac tggttcattt tcgatcaatt tcttcaattt
    13801 ttcttgttcc agctgtgctg gcaaatctgt tttatttaga agaatgatcc gtttcaatcc
    13861 acttgtcgct tccaataatt gttcatcttc tgctgtcagt ggttcgcttt ggtttagaac
    13921 aagtaagatc aagtctgctt ctgctaatgc ttttcggctt ctttcgaccc cgatccgttc
    13981 caccacatct tctgtttccc gaattccagc tgtatcgatt agcttgagcg gtaccccccg
    14041 aacattgaca tactcttcga ttacatctcg agtcgttcct gcaatatccg taacgattgc
    14101 tttttcttct cttaacaaat ggtttaacaa acttgatttg ccgacatttg gccggccgat
    14161 gattgccgta cttaaccctt cccgcagaac cttcccttgc ttggatgtag caagtagcgc
    14221 ttggattctt tgttggatca tctgcgcttt ttcaagcaat aacttcgtag tcaattcttc
    14281 gacatcatca tattctggat aatcaatatt tacttctact tgtgctaatg tctctaaaat
    14341 ctcttgtctc aatgagcgga tcaaagcaga cagattgccg tctaactgat taagagctag
    14401 tcccattgct ttgtctgttt ttgcccgaat caaatccatc acagcttctg cctgtgaaag
    14461 gtcaacacga ccattcaaga atgctcgttt tgtgaattcg cctggctcag ctaatcttgc
    14521 accttctctt aacaatagct gcaaaatttg attgactaca acgatcccac cgtgacaatt
    14581 gatttctact acatcttccc gcgtaaatgt ctttggtgct ctcatcacgg agaccatgac
    14641 ttcatcgaca agctgattgc tcttgggatc aacgatatgt ccataatgga tcgtatgact
    14701 tggtacttct gataatcgtt tgtttccaga ttgataaact ttatctgcta gttgcacagc
    14761 ttgatcgcca cttaaacgaa cgatactgat tgcaccttct ccaggtgggg tagaaattgc
    14821 cgcaatcgta tcaaattcta aagtaatttc agccattttt tcacttcctt tttcattttg
    14881 cgcacaaaaa aagtgcccac tccacccttt atgcacaata aacggtgaac ttcgcacttt
    14941 gactgcttgt tcttgattaa attctggtca tagattatca cacttgctat aaggaatcaa
    15001 gtgtttaaaa caattagatg aagttgagcc tgttctgctt ccattacctt atatttaatt
    15061 gtccttggat gtctctttgc gttctttggt ttattcgaag agaagatact gtataaacaa
    15121 ggctttatct ttcttaaaaa aaacggtaca ctaaatggta gcgctatcta aactatacac
    15181 ttttgaaagg agaaaatatg tgagcccgtt cagatctgct gcattggaag aagaaagtga
    15241 ccctcggatc aacgatgtcg ttttatctac agggaatatc aaccagaaat atatcgtccg
    15301 cgatatcgtt tttgttgtgg aatgcttaga agctgatttg tttgacccat cttttcccaa
    15361 agatgacctt ttcttaacaa cgaagaggaa attaaagacg aaggtacttg aatatggtgc
    15421 gaatgcagta atcaactgtc attttgagta tcagcaaaaa aaacgcgacg gaaagtattt
    15481 tctggaactt tttgcttatg gaacagtgat ccaatttatc caaacaacga ttggttaaat
    15541 gaaacagaca aaaaaagcgg atcaaaactg aagacagttt cgatccgctt ttttctatca
    15601 aagttagtga ctacttcatc gtatactctg ataagaatga tttctagtaa aatatacttg
    15661 gtttgaaaaa ctttgtcaac gtgatttatt caaaaagtaa tggaatcaat aatatttttt
    15721 agcaggttct actactaaat aacgataagg ttcctcacct tccgaatgtg tttttacatg
    15781 ttcatctttg cttaatgcag catggatttg tttacgttca aatgcaggca ttggttctaa
    15841 gaaaacaggc tgccctgtcc gttttacttt ttcagccgtg cgatcagcta aacgctgaat
    15901 gatctcttgg cgcttttcac ggtaattgcc tacattgact actacagaca gcttatttct
    15961 tgctaaacga tggatgaaga cttgtgccag atattgcaat gcattcagtg tttttccatg
    16021 tttcccaatc agtatgcctt gcttttgtgt ttctaaatgg aaaacaacca tattatttga
    16081 ttctctttcc attttgacaa gagctggagc atcaagctgt tttgaaattt cagtaagata
    16141 aactgccaat tccgttaaag ctgcttcatc acctagctca cttacttctt ctgttttttc
    16201 ttcaagaatc gtttcgttga ctacctcgat ctcagctgtt ggttcttctt ccaaaatttc
    16261 ttctacagtc tgttctactg tttcagaaac tttttcgctg atacttggct ggatcgaaag
    16321 ccgtgcattc tttcgaccca ttcctaggaa accttttttt ccttcttcca gtatttcgat
    16381 ttcgacttgg tctttggcca aacccaaatc agccagacct agttggacag cttcgtctat
    16441 tgttgcagct tcataaatcg gcattagcat tacctcccct attatttccg ttttttctta
    16501 ggattcattg ctttcttaag agcacgttct ctttcgcgga ttctccgtgc ttcttcttca
    16561 cgctcttttc gaattttgaa agggttgttg atcagcagcg tttgtacaac ttggaaggca
    16621 ttggaaacta cccagtataa tgaaagaccg ctggctaaat tgacacccat cactaaaatc
    16681 attacaggca tcacgaaatt catgatcttc attgatgcat tcgattcaat ttgactcatg
    16741 ctagataaat acgtacttgc aaaagtaaag attgctgcta aaatcggcaa aataaatgtt
    16801 ggatccgcgt tccctaagtt caaccataag aaatgccctt gtttcaactc tggtacacgt
    16861 gaaatagatt gccataacgc catcaagatt ggcatttgta ctaataaagg aagacaacca
    16921 gcatatggat tgacgccatt ttctgcatat aaacgctgct gctcttctct taacttactt
    16981 tgtgtttccg gatctcttga actatattgt tgctgcagtg cttttaattt cggttgtaat
    17041 tcttgcgttt tacgcatgct ctttgtttgg aaatgcatca acggcaatag gatcacgcgg
    17101 atgatcagtg taaataaaat gatccctatc cctgcattac caaatgacaa cgccttgatc
    17161 gcctcagcaa agtaataaac aatataacga tcccaaattc ctgtactctg tgcgctgact
    17221 tccccagttc cgcaaccgga taagacgatg accaaactaa cgataccggc catcaaaagt
    17281 aaacgtttgt atttcttcac ttactctttt cctctctgtc gattattttt gcaagtttta
    17341 agacgtggac taaattggag cgaacctcat cataagtcaa ccccttcaca cttggtctag
    17401 caatcacaat aaaatccaac tccggatcaa tttcttcttt tatagaataa actgctgctc
    17461 ggatttttcg tttgacttca ttgcggttta ccgcgttgcc gacctttttt cctacagata
    17521 aaccaactcg aaaatgaggc tgtcccggtt tttccaaacg gtaaacgacg aatttgcggt
    17581 tagcaaaaga atctcccgta tggaacacgg cttgaaactc tcgttctttt tttactcgat
    17641 acgcttttct cattagtttt ctttcctttt ctatctctca cttacgctca taatcatacc
    17701 ataattacga ttgtcctttc atcatttccc taaaaaagag tataaaaaaa accactgaaa
    17761 ccccagtggc ctaagctgaa agaacttttc ttcctttgcg acgacggcta gctaacacac
    17821 gacgtccatt tttagtactc atacgtttac ggaatccgtg aactttttga cgtttacgtt
    17881 tatttggttg gtatgttctt ttcatatatt ccacctccaa aatgttctta tctattttat
    17941 aaacacagac atactttgat agtataacga caaagtagtg attttgtcaa tataaaatag
    18001 aagatcgatt gttaaaactt atccacaaga ttattttttt ctatgggaat cctatgtgga
    18061 aaaagttatg cacagggatt atccacagat aataaaaggc tggttttaca cagaaaacac
    18121 acgtgtaaaa aaagtaatcc acaatcaggc gatttcctgt gtattccgtc tcctaactat
    18181 tggtatatct gtatttttta tccacagacg caagaggaaa tctattggtt tttacgagtt
    18241 ttccacctgt gcattagtgg tgtggaaaac tttttttgac aactgtgttt ttctcttttt
    18301 tctttttgtc gattgtgtgg ataattgtaa ttaaaaaatt tttttcttcg gtttctgtgg
    18361 aaaactattg aaaaaaatgc taaattagta ctagttattc actttttaat gaaggaggag
    18421 gccttatatg gtatccctcg atgctttatg gaatgaatta aaagcaacat accaaaagga
    18481 tttatcgcct gctagctata atacatggat cgaaacagcg aaccctcgaa cccttgatca
    18541 aaaccagctc gttgttgagg ttcctagcaa gattcataaa gaatattggg aaaaaaacct
    18601 ggcgacaaaa atcgttgaag tcggttatat gttatcagga aatgagatta tccctcgctt
    18661 tattactggc gaagaagcag agcaagaaga agttatagaa gaaaaaaatc caaaagttgt
    18721 ggcacctagt ccgctaaaaa aagccatgct gaaccctaaa tatacctttg atacgtttgt
    18781 cattggtaaa ggaaaccaga tggctcatgc agcagcgctc gttgtagcag aagatccagg
    18841 ttctatttat aacccgctct tcttttatgg aggcgtggga cttggtaaga ctcacttgat
    18901 gcatgcaatt ggtcatcaaa tgctgcaaag tcagcctaat gccaaagtaa aatatgtaag
    18961 cagtgaaact tttgctaatg atttcatcaa ttcgatccaa aacaaaacag cagaagagtt
    19021 ccgtcaagaa tatcgaaatg ttgatttact gctagtggat gacattcaat tttttgcaga
    19081 aaaagaagct acgcaagaag agtttttcca tactttcaat gccttgtaca atgaagggaa
    19141 acaaatcgta ctaaccagtg atcggttgcc aaacgagatt ccgaaactgc aagaacgcct
    19201 tgtttctcgc tttgcatggg ggctgtctgt tgatattaca ccgccggatc ttgaaacaag
    19261 aacagcgatt ttacgtaaaa aagcggcggc tgaacgtcta gagatcccag atgacacact
    19321 aagctacatc gcaggacaaa ttgattctaa cattcgtgaa ttagaaggtg cgcttgttcg
    19381 agtgcaagct tttgctgcga tgaatagtga agatatttcg actagtctag ctgcagacgc
    19441 actgaaaacg ttaaaatcag gaaaaggaca tcctcaattg tcaatcctgc agatccaaga
    19501 agaagtggca aagtattatc acatccaatt gaaagatctc aaaggaaaga aacgcgtcaa
    19561 atctattgtt gttcctagac aaatcgctat gtatttggca cgtgagctca cagataattc
    19621 tttaccgaaa atcggagctg aattcggtgg caaggatcat acaacggtta tccatgctca
    19681 tgaaaaaatc cagcagctga tggattctag tgtcagtatg caaaatgaag tatcagaaat
    19741 caaaaacttt ttactaaatt aatttgtcga ttctatcaac tgaaaatagc atgtggataa
    19801 aagaaaaaaa cgttcaaaag ttatacacac tttttccaca gctgtttttt cgtttctctc
    19861 ttacgttttt tgagttttcc acagaatcaa catgccctat tacttttatt attcttttta
    19921 actaataata aataaacaaa cattcaaagg agatacatca tgaaagttac tttaaaccga
    19981 gctagcttta tgcaggaatt gcaaactgtt caacgagcta tttcaagcaa aaccacgatc
    20041 cctattttga caggtgtaaa aatcacactg acacaagaag gtttgacttt gacggggagc
    20101 aacgctgata tatcaattga aacttttttg tctgttgaaa acgaaaaagc aaatatgcaa
    20161 atcgaatcta ctggttccat tgttttacaa gcacgtttct ttagcgaaat cattcggaga
    20221 cttcctgaag aaacatttac tttagaagtt ttagaaaata aacaagtagc gatcacttct
    20281 ggaaaagcga attttatcgt aaatggatta gatgcagata actatcctca tcttcctgtt
    20341 gtcgaaagcc ataaccagat gaaattacct gtacacgtat tgactaaact aatcaacgaa
    20401 acagtttttg ctgtctcaca acatgagagt cgtccaatct tgacaggtgt gcatttcatt
    20461 ttatctgata attctttatt agctgtagct actgattctc accgtctaag tcaacgcgtg
    20521 attccagtag aacaagcggc tgatcatttt gatattgtta ttcctggaaa aagtttgatc
    20581 gaattatctc gctcattaac aaatgaagaa gaaatcgtcg aaatcagcat tatggaaaac
    20641 caagtgttgt tcaaaacaga aacgatgtat ttctattccc gtttgctaga aggaaactat
    20701 ccagatacca atcgtttgat tccttcaagt tttaatacag aggttgaatt ttctgttcct
    20761 agctttttag cggcgatcga acgggcttct ttactttctc atgaaggccg taacaacatc
    20821 gttcgtttat ctattcgtcc agatgcggtt gttttatacg gaaattcgcc agaaatcggg
    20881 aaagtagaag aaagcttgag ttatacagca agcagtggcg atccattaga tatttctttc
    20941 aatccggatt atatgaaagc agcgttgcgt gcatttggag atatgagcat caaagtgaaa
    21001 tttatctctg ctattcgtcc atttacattg gaaccaacgg aagatggcgt tcaatttatt
    21061 cagctgatta ctcctgtacg tacaaattaa caatgatttt caatgttctt gaaaatttaa
    21121 ttgaaaagaa atagaaaaaa taaaatttta aacagaaggc cctaaaatct cttttaaggc
    21181 tttctgttta tttttgttta aatcaccttt tttagattaa atcgtcttat atggaaaaat
    21241 atatggataa atttgtttta ttgcgaaaaa aaaggtataa tagagtatac ttttaataat
    21301 gaaaatgagg gatgggcatt gaaacaacaa gtcattctaa atacggaatt catgacgttg
    21361 ggccaaatgt tgaaagaagt cacagttatc ggtagtggcg gacaagcaaa atggtatttg
    21421 gcagaaaaca ctgtgttagt cgatggcgaa ccagaaaatc gtcgtggacg caaactttat
    21481 cccggcatga tggttgaagt tccagaagta ggaacttttt ttatggtaaa aaaaggaaac
    21541 gacgataatg aggctgaata agctgtattt aaaaaattat cgaaattacg aagagctgaa
    21601 catcgaattt tccaagaacc tcgtcatctt tttaggagaa aatgcgcaag gaaaaacgaa
    21661 tcttttggaa agtatctacg tattagctat gacgcgaagt catcggacaa gtagtgagca
    21721 ggaattgatt ggctggactg acgatcaggc gatgattcaa ggagagatca caaaaggttc
    21781 atccactctt ccattagaaa tcttattatc taaaaaaggc cgaaaaacca agatcaacca
    21841 tatcgaacag aaaaagttaa gtacttacgt gggacaaatg aatgtcattt tatttgcacc
    21901 agaagatctt tctttagtaa aaggcagccc gcagaccagg cggaagtttt tagatatgga
    21961 attaggccag atcaatccaa tctatcttta tgatcttgtc caataccaag caattttgaa
    22021 acaaagaaac cagtacttga agcaattgaa tgaaaaaaaa caaactgatt tgctttattt
    22081 ggatattttg tccgaacaac tagcagcgtt tggcagcaaa gtattgaaag caagagcaca
    22141 gttcgtcaag cgattggagt tttgggctaa ttctttgcat cagcaaatca cccatcaaaa
    22201 agagcagctg gaaatcgagt atctcaccgc agtcgattct ttagaaacac atacgcaaga
    22261 gcaaatccaa gaacaatttc tcgcactttt gaaccaaaat aaaaagaaag acctttttcg
    22321 gggaacaacg acagttggtc ctcatcgtga cgatctttcc ttttttatta atcagaaaaa
    22381 tgtgcagact tatggatcgc aaggacagca aagaacaact gcactaagtg tcaaattagc
    22441 agaaatcgat ttgattaaag aagagacagg ggaatatcca atccttttgt tagatgatgt
    22501 gatgagcgag ctggatgata atcgtcaact ccatctgcta gaaacaatcg aaggaaaagt
    22561 tcaaacgttc ttaacaacaa cgacacttga ccatgtaaaa aataaaatga ctgttgaacc
    22621 tgagattttt tatgttcaac aagggaaact aaaaggagaa gaaactgaat gacagaagaa
    22681 agaagtttag tagaacgcgc taaagagtat gatgccagtc agattcaagt acttgaagga
    22741 cttgaagccg ttcgtaaacg acctggcatg tatatcggtt cgactagttc tgagggtctg
    22801 catcaccttg tttgggaaat tgtcgataat tcgatcgatg aagtattagc aggttttgca
    22861 acgaaaatcc atgtgattat tgaaaaagac aacagtatca ccgttattga cgacggtcgg
    22921 gggatacctg tagatatcca ggcaaaaact ggacgtcctg cagttgaaac ggtctttact
    22981 gttttacatg ctggaggaaa attcggcggt ggtgggtaca aagtttctgg tggacttcac
    23041 ggagtaggat cttctgttgt taacgcgctt tctacacagc ttgatgtcaa agtatacaaa
    23101 gaaggaaatg tgtactatca agaatatcgt cgtggagctg tagtggacga cttaaagatt
    23161 atcgaacaaa cggatcgcca tggaacaacg gttcacttta cgccagaccc tgaaattttt
    23221 acagagacaa ctgagtttga tttttcaaaa ttggctaccc ggatccgtga acttgctttc
    23281 ttgaaccgcg ggatgcgtat atcgattgaa gacaaacgag aagaagaacc agtcatcaat
    23341 gaataccatt atgacggcgg gattaaaagt tatgtcgaat acttgaatgc aaacaaaacc
    23401 gtgatttttc cagaacctgt atatttggaa ggtgaacagc aagatatcgc agttgaagtc
    23461 tctatgcaat atacagacgg ctaccattcc aatatcatga gttttgccaa caatatccat
    23521 acgtatgaag gcggaacaca tgaatcagga ttcaagactg ctttaactcg tgtgataaat
    23581 gattatgccc gaaaacaaaa gctgatgaaa gaaaatgacg acaacttgac cggtgaagac
    23641 gtgcgagaag gattgacagc tgttatttct atcaagcatc ctgaccctca attcgaagga
    23701 caaacaaaaa caaaattggg caattccgaa gtacggacag tcactgatcg gttatttagc
    23761 gaacatttca tgaaattctt gctggaaaac ccaagtgtgg gccgtcaaat tgttgaaaaa
    23821 ggattactag cttccagagc tcgtctagca gcgaaacgtg cgcgtgaagt aacgagacgt
    23881 aaaggtgctt tagagattag taatctgcca ggaaaattag cggactgctc tagcaacgat
    23941 cctgaaaaat gcgaattgtt catcgtcgaa ggagattcgg ccggcggatc agctaagcaa
    24001 ggtcgtaatc gagagtttca agccatcttg cctattcgtg ggaaaatctt gaatgttgaa
    24061 aaagcaagca tggacaagat cttggcaaat gaagagatcc ggtctttatt tactgctatg
    24121 ggtacagggt ttggagaaga ttttgatgta tcgaaagctc gctaccacaa attagttatc
    24181 atgactgatg ccgatgtcga cggcgcgcat atccgtacat tgctgctgac tcttttttat
    24241 cgttacatgc gtcctattgt tgaagctgga tacgtctata ttgctcagcc acctttatac
    24301 ggggtaaaac aagggaagaa tattacgtat gtacaaccag gaaaaaatgc agaagaagag
    24361 ttgaaacaag tcgttgcttc acttcctgcc tcaccaaaac caagcgttca gcgttataaa
    24421 ggacttggag aaatggatga tcatcaacta tgggaaacaa ctatggaccc aagcaatcgg
    24481 atgatggccc gcgtcagtgt ggacgatgct attgcagcag atcagatatt tgaaatgttg
    24541 atgggggatc gagtagaacc ccgtcgtgca ttcatcgagg aaaatgcaca ctatgtgaag
    24601 aacttagata tttaatcatg aaggaggaaa aagaatgagt gaagaaatca gagaaaacat
    24661 ccatgatgtc aatctaacca gcgaaatgaa agactccttc atcgattatg cgatgagtgt
    24721 tattgtcgca cgtgcacttc ctgatgtcag agatggtctg aaacccgttc atcgccgtat
    24781 tttatacggc atgaacgaat taggtgtaac accggataaa gcccataaaa aatctgccag
    24841 aatcgtcgga gatgtcatgg gtaaatacca tcctcatgga gactatgcga tctatgagtc
    24901 aatggtacgg atggcacagc cattcagcta ccgttatatg ctagtggatg gtcatggaaa
    24961 ctttggttct gttgacggtg acggtgccgc cgctatgcgt tataccgaag cacggatgag
    25021 caaaatcgct acagaaatgc tgcgtgatat caataagaac actgttgatt tccaaagcaa
    25081 ctatgatgat acagaaaaag aaccagttgt actacctgct cgcttcccga atcttttagt
    25141 gaacggaaca acggggatcg ccgtaggtat ggccacgaat atcccacctc ataacttgtc
    25201 tgaggtagta gcggctatcg atttgctgat ggataatcct gatgtgacga ccaatgaatt
    25261 gatggaagta ttacctggac cagattttcc aacaggtggt ttggttatgg gaaaatcagg
    25321 aatccgaaga gcgtatgaaa caggtaaggg ttctattacc gtacgtgcaa aagttgagat
    25381 tactgagatg ccaaatggta aagaacggat catcgtgaca gaattgcctt atatggtcaa
    25441 taaagcaaaa ttgatcgaac ggatttctga actgcacaga gacaagcgta tcgaagggat
    25501 cacagatttg cgggacgaat cttcccgtga aggtatgcgg atcgtcattg acatccgtcg
    25561 tgacgccagc gcatctgtta tcttgaataa cttatataaa ttgacatcct tgcaaaattc
    25621 atttggattc aatatgttgg cgatcgaaaa aggtgtgccg aaagttttga gcttgaaaca
    25681 aattcttgaa aactatatcg aacatcagcg cgaagtcatc acaagacgga cagtctttga
    25741 aaaagaaaaa gcagaagcaa gggcacacat tttagaaggt ttgcgtattg ctttggatca
    25801 tatcgatgaa atcattgcga ttatccgcgg gtctaaatca gatgacgaag caaaagcgac
    25861 gatgatcgaa cggttcgatt tatccgatcg ccagtctcaa gctattttag atatgcgttt
    25921 acgccgattg accggtttgg aacgggaaaa aatcgaaaat gaataccaag aactcttaaa
    25981 actgattgaa gatttaactg acattctggc aagaccagaa cgtgttacag aaatcatcaa
    26041 gaatgaatta gcagacttga atcaacgatt tggtgataaa cgacgcacag aattactagt
    26101 cggtgaggtc ttaagtctgg aagatgaaga tttaatcgaa gaagaagaaa tcgttattac
    26161 attgacaaac aatggttaca tcaaacgttt ggcaaataat gaattcagag ctcaacgaag
    26221 gggtggacgt ggtgtccaag gaatgggtat ccacaatgac gacttcgtca aaaatctggt
    26281 ttcttgttct acccatgata cattgttatt ctttacgaat aacggaaaag tgtatcgggc
    26341 aaaaggatac gaaattccag aatacgggcg cacagccaaa ggaataccaa ttatcaattt
    26401 actaggtatc gattcgagtg aaagcatcca ggcaatcatt gcggtgacgg gagaagcaga
    26461 agaaggccac tatctcttct ttacgacgcg tcaaggtaca gtaaaacgta cttctgtcac
    26521 tgcttttgct aacatccgaa gtaatggtct gatcgcaatt ggtttgaaag aagacgatga
    26581 gctcgtcaat gtgctactga cagatggtca atctaatatc attattggaa cacataacgg
    26641 ctactctgtc actttcaaag aagaagcagt ccgcgatatg ggacgtacag cttccggggt
    26701 tagaggaata cgcctgcgtg aggaagatta tgtcgttggg gcagccttaa tgaaggaaga
    26761 tcaagaagta ttagtgatca cagaaaaagg ttacggaaaa cggacaaaag tatcggaata
    26821 tccagtgaaa ggccgcggag gtaaagggat caaaacggcg aacatcactg aaaaaaatgg
    26881 tccactagct ggattgacca ctgtttctgg agatgaagac atcatgctga ttacagataa
    26941 aggtgtcatt atccgtttca acgtatcgac tgtttctcaa actggacgtt caacattagg
    27001 cgtacgtttg atgaagatgg aagctgatac gaaagttgtc acgatggcag cagtcgaacc
    27061 agaagctgca gaacaaacag ttgaagaaca aggatcagaa gaatagccat caagactaat
    27121 tgtgtaagct agcaagaatg accgattaaa aaatctggtt attcctgctg gcttttttga
    27181 ttagttgaat ttctcttaaa atattcgaaa ctgttcattc tgtattgctt ttgaccaaaa
    27241 tagagtgtat aatcagagct tgtgagtagt ctaattaatt agataactct ccttgctctt
    27301 aggaacctaa gagccgaaag tccacaggga ggtgaatatc aatggaaaac acgaaatatg
    27361 aaatcatgta cattattcgt ccaaacattg atgaagaagc aaaaactgct ttaatcgaac
    27421 gtttcgatgc aatcttgaaa gataacggag cagaagttat cgaatcaaaa gattgggaaa
    27481 aacgccgttt cgcttatgaa atgaacggtt accgtgaagg tatctatcac atcgttaaag
    27541 tatcttctcc atcatcagca aatgctgtca atgaatttga ccgtcttgct aaaatcaacg
    27601 acgatatcat tcgtcacatg attgttaaag aagaagctta attgttgttt catgtgaaac
    27661 aatagaaaac gatgagagga gatgactatt ttgattaata atgttgtatt agttggacgt
    27721 ctgacgaaag atccagattt gcgttacaca gcaagtggta cggcagtcgc aacatttact
    27781 ttggctgtga atcgtaattt tacaaaccaa agcggcaatc gcgaggctga ttttatcaac
    27841 tgtgtgattt ggcgcaaatc agcagaaact ttagcaaact atgctcgtaa aggaacactt
    27901 ttaggtgtta ctggacgtat ccaaactcgt tcttatgata atcagcaagg acaacgtgta
    27961 tacgtgacag aagtagtagc tgacaacttc caattgctgg aaagtcgttc tgcatctgaa
    28021 aatcgccaac aaagcggcgg attccaaagt tctggtcaat cagctggcgg atttggcgga
    28081 aacaataact ctaatcaaac atctcaatca tccaacggta tgcctgattt cgaccgtgat
    28141 acttctgacc cattcggctc aagttcaacg atcgacattt cagacgatga tttaccattc
    28201 taaacgaatt tgataggagg gaaatagaat ggcacaacaa agaagaggcg gacgtaaacg
    28261 tcgtaaagta gactacattg ctgctaacca tattgaatat atcgattata aagatatcga
    28321 attactaaaa agatttattt cagaacgcgg taaaatcttg ccacgtcgtg taactggtac
    28381 aggcgctaaa aaccaacgta aaattactgt tgcaatcaaa cgcgctcgta tcatgggctt
    28441 gctacctttc gttagcgaag actaataaat cctaggaatt gaatgactga tccttttgat
    28501 cagctattca attccttttt ttctgtaaaa ataacctcta ttcgtttagt cgctcgaatg
    28561 acgaaagtta tggtaaaatc aaattagtca tatatgggag gaaaattatg gaaaataaaa
    28621 acaatcattt atccccgaaa cctctcttcc ttatcctgtt tatattaatc gaagtattgg
    28681 tagtcttaat tttaccattt cgttttgtta gcgcttcggt cattctattg atgaatttat
    28741 ggggtgttcg ctattggttg aaaatgaaac gttggcttat tttaaataaa cgtgaaaaaa
    28801 tccagcaggc ttcgactgta gctgaaacca atctagctta tgtctcagaa gtcatgcctg
    28861 taggagtgat tgcttatcag ccatcaagcc aaaaaatcga atggctcaat ccattagcat
    28921 tgaaaatcaa agatcaaagt ggattaacag aggaagaatt gctagatact ttttttactg
    28981 caaaagaaaa aaagaaaaac caagtctatc tagcagataa aacatttcag tttgatatgg
    29041 atacagtaaa gggtgttgtt tattttaatg atattaccca aaaccagttg ttaaaactcc
    29101 atcagacgaa cagtcagccg gtgattggta ttttatctat tgataactat gcagatgcca
    29161 ttgataaatt ggatgacaaa gagatttctt atttgaactc tttcgtcact acactagttt
    29221 ctgactggat gaatgaatac catgtctttt tcaaaagaat caacgcagaa cggtttttct
    29281 tcgtagcaca gatgatcgat ctgaaaaaga tgatggataa aaaatttgat ttgctggatc
    29341 gctttcgagc agaagctgaa aaacagagct cgcctatcac ccttagtatg gggattgctt
    29401 atggctcaag aaccctcgat gaaatcggtg gagaagctca gaataattta gatgttgctt
    29461 tagtccgtgg aggcgaccaa gttgtcgtga aagaggtaaa agaaggagca aaacctgttt
    29521 attatggggg gaaatctgct aagaatgtca aacgcacgcg tgttcgttct cgagcgatga
    29581 gtaccgcttt gcgtcggatt tttgatgaaa caggcgatgt gtttatcatg gggcacaagt
    29641 ttccggatat ggatgcttta ggctctgctt ttggtgtcag ttgtctggca cgcttcaacc
    29701 agaaaaaagc ttacatcgtt atcaacgaga acgagatcat tccagatgta gaacggtgtc
    29761 ttgaagaaat ccataagcat cctgaattag aaatgcaact aatctctcca gaacgtgcca
    29821 tagagctgaa aaaagaagag gacctcctcg tgatggtgga ttaccataaa ccttctctat
    29881 cgatctctca gggcctatat gagcaatttg ataagattgt gatcattgat caccatcgtc
    29941 gtggggaaga gtttccaagt aaacctctgc tgacttatat cgaatcttca gcttcttcag
    30001 cttcagagct agtttctgag ctgatccaat accaaagcag caaaacaaat cgcttagatc
    30061 gttttgaggc cacattattg cttgcaggga tggttgtaga tacaaatagt ttcaatgtcc
    30121 gtacaactgc acgaactttt gacgttgcca gctatttaag gtcttgtgga gcagacgcat
    30181 ccttagtaca atatttattg agctcggatt tagcgacata cttggagatc agcaatttga
    30241 ttgctggaag cgactatgtg acttctgata ttgttgtggc ggcagggaca gacgaaaaag
    30301 agtataatag tgttatagca gcaaaaacag cagatacatt gctttcgatg atcaatatca
    30361 atgcagcttt cgtcattaca aagcggacag acggcttgat tgggatcagt gccagaagta
    30421 ccggcacgat caacgtccaa atcatcatgg aatccttagg tggcggagga cacttcacaa
    30481 acgcagctac tcaactggaa aatacgacag ttgaggaaac aaaacagaag ctatttgctg
    30541 caatcaaaga aaatatgaat caaatatatg ataaggagta atccaaatga aagtaatttt
    30601 tttacaagat gtaaaaggaa aaggtaaaaa aggggacatc aaagaagttc cagcaggtta
    30661 tgcacaaaat tatctaatca aaaataactt ggcaaaagaa gcaacgaaaa gcagtatcag
    30721 tcagttagca ggacaaaaga aagcacaaga aaaacacgaa gctgaaatct tggaagaagc
    30781 taaaaagtta aaagaattct tggaaaaaga agatactgta gtcgaattga aggcgaaagc
    30841 aggggaagac agccgtttgt ttggttcgat cccttcaaaa caaattgctg aagggttaaa
    30901 caaacaatac aaaatcaaat tagataaacg aaaaatcgaa ttagaaaatc cgatccgttc
    30961 actaggctac acaaacgtac ctgttaagct tcatcatgag gtgacggcta cgatcaaagt
    31021 tcatgtcgta caagaataac aaatcttgat agcgatgttc agctggtaag tataaccaga
    31081 aggaaaaaag acaaaccgaa atttatctga gaaaacagct gcagcaatga aagcaaaagc
    31141 tgcaaatcga tttcagattg atttcggttt cttttttggg aacagcaaaa tagcataaag
    31201 aaaacgagta taataaaaga gaactatttt tttattacac tttcggtacg aatgatgact
    31261 tttctctcat tttcttgtaa aatgaagcaa ttgaagacaa ggagcgatac gtatgaatga
    31321 agtgtggcag gatcgaatcc caccgcaaaa tattgaagct gaacaagcgg ttttaggctc
    31381 tgtttttctt gacgcagaaa cgatcattga tgccatggag tacatagagc caaaagactt
    31441 ttatcgtcgt ggccatcaaa ttatttttga gacaatgatc gagttaaatg atcgtaatga
    31501 agcgattgac gtggttactg tcaaagaccg gctggaacaa gccaatttgt tagaagatgc
    31561 cggtggttta agttatctct cagacttggc attagcagtt ccgacagcag caaatatcgt
    31621 ctactatgcg aaaatcgttg aagaaaaatc attgctgcgt acattgatcc agacagcaac
    31681 aggaatcgtt acgaaaggat tcgagcaagg agaagaagtt caaacaattt tagatgatgc
    31741 cgaacgcagt atcttggagg tttcggaaaa aagaaatcga agcggctttt tatctattgc
    31801 tgaagtactg aactccacga ttgaaaatat cgatcgacta gctcaaaaca atgaagaaat
    31861 cacaggtcta ccaactggct atcaaggtct ggataaaatg acagcaggtt tccagcccga
    31921 agaactaatc attcttgctg cacgtccagc ggtaggtaaa acagcgtttg cattgaatat
    31981 tgctcaaaat gtaggaacaa aaacagatcg ttctgttgcg atatttagtt tggaaatggg
    32041 ggcagaatct cttgtaaatc ggatgctttg tgcagaaggt tcgattgagg ccagccattt
    32101 acgtacaggt cagctttcgg aagaagaatg gtcgaatctg atcgttgcga tggggagtct
    32161 ttctcgagca aatattttta tcgatgatac accagggatc aaaatctctg aaatccgtgc
    32221 gaaatgtcgg aaactagcgc aagaaaaagg caatctaggc ttgatattga tcgattattt
    32281 gcagttgatt gaaggtaacg gtaaagagaa ccgtcaacaa gaagtatcag atatctctcg
    32341 ccaactgaaa aaactggcga aagagctgaa agtacctgtc attgctcttt cacagttatc
    32401 tcgtggagta gaacagcgac aagacaaacg accagtgcta agtgatattc gtgaatccgg
    32461 atcaattgaa caagatgcgg acatcgtagc cttcttgtat cgagatgatt attatgaaag
    32521 agaaggcgga gatgacgatg atggaaacga gccaaaaaat gacaacgtca tcgaagtcat
    32581 catcgagaag aaccgaagcg gtgcacgagg aacagttgaa ctactgttta tcaaagaata
    32641 caataagttt tcgtcgcttt cgccaagaga agaattttaa agaagaggtt gtgagccttg
    32701 agcaatcccg agagctctcg taagaagatg caaggagtaa ccgcaggaac gactagagta
    32761 cttaatttaa acagatctcc aaagggattg tgacaaaagt cttgtcacag tccctttttc
    32821 cgaataaacg gtgttcaatc agctgaccct tggtattagc tcagcaaacg tcaaaatatg
    32881 aggaactatt ttgacgtttg tttctctaat actcaggtca aaacgctgat cttacgacct
    32941 cttttcctta aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatcatcaaa
    33001 catgagaagc agttttttga aattgctcct tgtgactcag agttaaacgg caggctcaca
    33061 acctctttct taaacggtgt tcaaaagctt gcatctgcgg taatacgccc aatcaaacga
    33121 aaaatatgag aagctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt
    33181 cacaacctct gattcacggt gttccaaaag tagcttcttc gaaaaataag tcgaaatttc
    33241 acaaaaattt gagaagcaat tttcgtacat tccttcttat ttcttgaagc tgaccacttc
    33301 tgttacaacc tcttttttaa taaccattct aaaaagataa agttcgtttt ttaagcgttt
    33361 ttataaaata aaaataaaaa tcattcgatt ttatttgaaa tatgtatcta ttattgatac
    33421 aatggttcag gattaaataa tatagaacga ggtgttcaca tgtcatctgt agtagtagta
    33481 ggtacgcaat ggggcgatga agggaaagga aaaatcacag actttctcag tgaaaatgct
    33541 gaagtgattg cacggtacca aggtggcgac aatgcagggc acacaattaa gtttaacggt
    33601 gtgacttata aactgcattt gattccttct gggatttttt ataaagacaa gatcagtgtg
    33661 atcggaaacg gcgttgtagt caatcctaaa tcattggtta aagaattagc ttatttacat
    33721 gaaaaaggtg tagaaacaag taatttgcgt atttctgacc gcgcgcacgt aatcttgcct
    33781 tatcatatca aattggacca attacaagaa gatgctaagg gcgaaaataa gattggtaca
    33841 acgattaaag gaatcggacc tgcctatatg gataaagcag ctcgtgtagg tatccgtatt
    33901 gcagacttat tagataaaga aatttttgaa gaacgtcttc gtttgaatct agatgacaaa
    33961 aaccgtcaat ttgtaaaaat gtttgattca gaaccacttg attttgacga tatttttgaa
    34021 gaatattatg aatacggaca acaaatcaaa caatacgtca cagatacttc cgttatcttg
    34081 aatgatgctt tggatgcagg caaacgcgtg ttgtttgaag gcgcacaagg tgtaatgctg
    34141 gatatcgacc agggaactta tccttttgtt acttcttcta acccagtagc cggtggggta
    34201 acaatcggaa gcggtgtagg gccttcaaaa atcaataaag ttgtcggcgt ctgcaaagcc
    34261 tatacgtcac gtgtcggtga tggaccattt ccaactgaat tatttgatga aacaggagaa
    34321 caaatccgcg aagtaggacg agaatacggt acgacaactg gtcgtccgcg tcgtgtaggc
    34381 tggtttgatt ctgtagttat gcgccattct aaacgagttt caggtattac caacctatct
    34441 ttaaactcga tcgatgtatt aagtggattg gaaacagtaa aaatttgtac ggcttatgaa
    34501 ttagatggcg aactaatcta ccactatcca gcaagcttga aagagttgaa ccgctgcaaa
    34561 ccaatctatg aagaattgcc aggctggtca gaagacatta caggatgcaa aacacttgca
    34621 gaactgccag aaaacgcacg taactatgta cgtcgtatct cagagcttgt tggtgtacgt
    34681 atctcaacat tttctgtcgg acctgataga acacaaacaa acatcttaga aagcgtctgg
    34741 gcgcaaatct aaggataaaa acgacgactg aatcaggacg ttcttaccag agcgagtcga
    34801 agtgtttatc tatacgataa taataacttg aacactgttt attcagaata agggactgtg
    34861 acaaaa
//
LOCUS       DASHRQ010000026        34522 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.026, whole genome shotgun sequence.
ACCESSION   DASHRQ010000026 DASHRQ010000000
VERSION     DASHRQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.026"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(133..306)
                     /locus_tag="U2M88_001364"
     CDS             complement(133..306)
                     /locus_tag="U2M88_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8373754.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="U2M88_001365"
                     /pseudo
     CDS             754..3116
                     /locus_tag="U2M88_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="U2M88_001366"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="U2M88_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEM8373755.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="U2M88_001367"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="U2M88_001367"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEM8373756.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="U2M88_001368"
     CDS             5704..6489
                     /locus_tag="U2M88_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM8373757.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="U2M88_001369"
     CDS             6502..7353
                     /locus_tag="U2M88_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEM8373758.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="U2M88_001370"
     CDS             complement(7477..7929)
                     /locus_tag="U2M88_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8373759.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="U2M88_001371"
     CDS             complement(8394..8762)
                     /locus_tag="U2M88_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEM8373760.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQMISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="U2M88_001372"
     CDS             8912..9394
                     /locus_tag="U2M88_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEM8373761.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="U2M88_001373"
     CDS             9431..10441
                     /locus_tag="U2M88_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEM8373762.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(10556..11269)
                     /locus_tag="U2M88_001374"
     CDS             complement(10556..11269)
                     /locus_tag="U2M88_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373763.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(11459..12049)
                     /locus_tag="U2M88_001375"
     CDS             complement(11459..12049)
                     /locus_tag="U2M88_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEM8373764.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            12533..12760
                     /locus_tag="U2M88_001376"
     CDS             12533..12760
                     /locus_tag="U2M88_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEM8373765.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            12776..13942
                     /locus_tag="U2M88_001377"
     CDS             12776..13942
                     /locus_tag="U2M88_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEM8373766.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            14176..15219
                     /gene="hrcA"
                     /locus_tag="U2M88_001378"
     CDS             14176..15219
                     /gene="hrcA"
                     /locus_tag="U2M88_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEM8373767.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            15313..15876
                     /gene="grpE"
                     /locus_tag="U2M88_001379"
     CDS             15313..15876
                     /gene="grpE"
                     /locus_tag="U2M88_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEM8373768.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            15927..17756
                     /gene="dnaK"
                     /locus_tag="U2M88_001380"
     CDS             15927..17756
                     /gene="dnaK"
                     /locus_tag="U2M88_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEM8373769.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            17907..19073
                     /gene="dnaJ"
                     /locus_tag="U2M88_001381"
     CDS             17907..19073
                     /gene="dnaJ"
                     /locus_tag="U2M88_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEM8373770.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(19155..19820)
                     /locus_tag="U2M88_001382"
     CDS             complement(19155..19820)
                     /locus_tag="U2M88_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8373771.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            19796..19969
                     /locus_tag="U2M88_001383"
     CDS             19796..19969
                     /locus_tag="U2M88_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373772.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(20014..20751)
                     /locus_tag="U2M88_001384"
     CDS             complement(20014..20751)
                     /locus_tag="U2M88_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEM8373773.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(20753..22255)
                     /locus_tag="U2M88_001385"
     CDS             complement(20753..22255)
                     /locus_tag="U2M88_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEM8373774.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            22514..24508
                     /gene="uvrB"
                     /locus_tag="U2M88_001386"
     CDS             22514..24508
                     /gene="uvrB"
                     /locus_tag="U2M88_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEM8373775.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            24518..27334
                     /gene="uvrA"
                     /locus_tag="U2M88_001387"
     CDS             24518..27334
                     /gene="uvrA"
                     /locus_tag="U2M88_001387"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEM8373776.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            27597..28862
                     /locus_tag="U2M88_001388"
     CDS             27597..28862
                     /locus_tag="U2M88_001388"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEM8373777.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            28866..29597
                     /locus_tag="U2M88_001389"
     CDS             28866..29597
                     /locus_tag="U2M88_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEM8373778.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            29732..30616
                     /gene="rapZ"
                     /locus_tag="U2M88_001390"
     CDS             29732..30616
                     /gene="rapZ"
                     /locus_tag="U2M88_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEM8373779.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            30613..31611
                     /locus_tag="U2M88_001391"
     CDS             30613..31611
                     /locus_tag="U2M88_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEM8373780.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            31645..32580
                     /gene="whiA"
                     /locus_tag="U2M88_001392"
     CDS             31645..32580
                     /gene="whiA"
                     /locus_tag="U2M88_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEM8373781.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            33123..33773
                     /locus_tag="U2M88_001393"
     CDS             33123..33773
                     /locus_tag="U2M88_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373782.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIEGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            33960..>34522
                     /locus_tag="U2M88_001394"
     CDS             33960..>34522
                     /locus_tag="U2M88_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HEM8373783.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        11453 a         5831 c         7324 g         9914 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcatttg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaaatg ctgtcagctt ctttttgtat aacatattag aaagtctaat
    10561 tatctataac tgtcacatcg attaactgtt tcacgtcatt gggatttcct gtaggaatag
    10621 ctactacaga tgcattatca atgatttctt gagttgtttc tctaatgaac gctggataag
    10681 atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg attgtcccgt
    10741 catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg agcataacca
    10801 catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt cctgcaggat
    10861 aatgaataac atgtaattcg aaatcacccg atgtatctct aaaagtatta cctctaatag
    10921 ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac ggatctattg
    10981 actgagaaga ctgtgaggag gttgtgtcta ttgttgaaga agaaatattt gtctcaactg
    11041 tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt gaagatgtat
    11101 tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg gtttgtctga
    11161 ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga gacgtacatc
    11221 ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata taaaatacct
    11281 cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt aaaaccttgt
    11341 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
    11401 tcatcagtaa aattacttgt cacactattg ataaggagta tttaattgaa gagaatgatt
    11461 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
    11521 tcgttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
    11581 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
    11641 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
    11701 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
    11761 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
    11821 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
    11881 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
    11941 tagaagcagt tttgttttgc catttctcat tttggtttag tgtgctagta atataagcac
    12001 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
    12061 cctttactat ctataacaag gacaacagtt ttaacctaca gacattcatt aaaattgggt
    12121 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagata gtcaacttaa
    12181 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
    12241 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tattaaaaga
    12301 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
    12361 tgaaaacaac cctagagata tataactagg taaccagttt aatgaaagaa aaaatcatcc
    12421 aagaagtcga ttcttggatt ttttagtgga tttgttcgcc aaatacgttt agtatgcgta
    12481 tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt aaggaggaat ctgtgaaaat
    12541 agcttttcat atttttgcag attttgactt aatgccgaag gtcagctttg cgattaccgt
    12601 ttatcgagag gctgtaaaag caccgtgtag ctccgatgac aataagcaag catttttaga
    12661 gctaggtttc cttgattcta aatatgcggc ttatttcaat gagttggtgc ttgaacgcga
    12721 ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa ggagaaagaa tttcaatgat
    12781 agaaacaata aatcccgcag aaaacaaggt ttctgcgtac attcatatcc cattttgcga
    12841 gcatatctgc tattactgtg attttaataa agttttttta gaagggcagc cagtagatga
    12901 gtatgtggaa atgctgctga atgaaatgaa aataatgcta gaaagtcatc cgacagcgaa
    12961 tttagaaaca ctttacgtag ggggcgggac accaacttct ttatctgcta aacaacttga
    13021 tcgactgtta tcaggtgcta gagagatcct gccttttcaa aatggtaatg agtttactgt
    13081 agaagcgaat ccaggtgatt tgacccgtga aaaacttcaa gtaatgaaaa actatggcgt
    13141 caatcgttta tccatgggag tgcagacatt tgataatcgc ttattgaaaa aaattggacg
    13201 taaacacacc gcagaagatg tgtatcaaac tatgcaattt ttagaagcag aaaattttac
    13261 caatgtaagc atcgatttga tttatgcatt accgggacaa acactagaag gatatctgga
    13321 tactttggaa aaagctttgg ctttggatct gcctcactat tctttgtact cattgatcct
    13381 cgaaaacaaa acaatgttca tgaactgggt acgacaagga agactcgaac tgcctgatca
    13441 ggaaacggaa accagaatgt ttgaagagac gattgaagca atggaaaagc atggacgtca
    13501 ccaatatgaa ataagtaact ttgggctctc cggacatgaa tctcaacata acctcatgta
    13561 ctggaacaat gaccactatt ttgggtttgg agctggtgct agcggttatc ttggagatat
    13621 tcgttaccgc aacaaaggac caatccaaca ctatttacgc ccattacgag caaatgaacg
    13681 cccggtattg gaagaagaaa aattgacacg aaaaaatcaa atagaagaag aaatgttctt
    13741 aggactaaga aaaaaaacag ggattactaa aaaacatttc tatgatcgct atcaaaaaac
    13801 aattgaatcg gtgtacggtg atgtgctaag ggaattagag caagaagggt tacttgttaa
    13861 tgaagaagac cgtgtttatt tgacaccaag agggaccttt ttggggaaca atgtgtttga
    13921 gcgctttttg tttgaaaaat agtttaaaga atatttgttt tttatctgaa tttaaataaa
    13981 aaaagttcaa aaatacaata aaggactatt gaaacgttga tataactgga tttacatcaa
    14041 ttatttagaa ttagcactct tgtgtttaga gtgctaaaaa atggagtctt tctcttgaca
    14101 ggagaagggc ttcttgctat atttataagt ggattagcac ttagaatata tgagtgctaa
    14161 tgagaggtga ggaagatgtt aacaaagcga caaaacgaca ttttacgtct gatcatccaa
    14221 cactatacga gtagtggcgt gcccgttggt tccaaaacat tgatgtctga gggagtcaag
    14281 gccagctcag caacgattcg caatgatatg aaagcattag aagatgaagg attgctgttg
    14341 aagactcact cttcttctgg gcggattcca tctgctttag gctatcgtta ttacgtggat
    14401 catttgttaa aacctgctcg cgtagcagaa gatgatttgc tgctgattcg tcagtctcta
    14461 ggcagagaat ttcatgaaat caatgagatt attaagcaat ctgctgagat attatctgag
    14521 ctgacaagct atacgacttt ttcattggga cctgaaataa aagaccgccg actgactggc
    14581 tttcgtatcg tacccttgaa taatcgacag attattgcta tcgtcgtaac agatatgggg
    14641 aatgtagaaa gccaagtgtt cactcttccc gaatatatgg gaagtcagga actagaaaaa
    14701 atggtacaga ttgtaaacga ccgtctagtc ggagatcctc tgatgacggt gtaccataaa
    14761 cttcgtacgg agattccgat gattcttcat aaatatttcc aaacgacaga agggatgtct
    14821 cagttatttg acaccgttct tggtcatgtt tttgaagata aggtttatgt cagtggacaa
    14881 atgaatctct tggattatga gccccgtcaa gatcttgatc agttcaaatc gatgttttct
    14941 tttatgaaag actcagacgg acttacacaa atgatcactc cgatggatag caatattcat
    15001 atcaagattg gtgctgaatt aggcaatgat ttattgcaga atatgagtat gattcaagca
    15061 agttacgaga tagatggtca tggaagaggt acaatagcct tgttagggcc tactagtatg
    15121 ccttattctc ggatgttcgg tctagtcgat gtttatcgaa gagaactagc agctaaactt
    15181 gctgattact accgttcgct tgacttgtca aactcgtaat gtgaataacg aagctatttg
    15241 cagctgatcg ttctctcttt cttagtaaga gcaatcaaac agctgactga ataatgaaag
    15301 gaagactgta tcgtgaaaga aaatcaagaa gaactagata aagaaatgac agatgctcag
    15361 ccagaaccgg aaatcgatgt cgaagctgca gaagattcgg gtatttcaga agctgaagcg
    15421 gaagaattcg aaacagcgaa attaaaagct gagttagaag aaatggaaga taaatacttg
    15481 cgggctcgtg ctgaaatcgc aaatatggca aatcgaggaa aaaatgagcg tgagcaatta
    15541 caaaaatacc gttcacaaga tttagccaaa aaattacttc catcaattga caacctggaa
    15601 cgtgcattag ccacagaagt gtcagatgat caaggggcag gtttgaaaaa aggcgtggaa
    15661 atggtcttgg aaagtttgcg taacgcactg gaagaagaag gaattgaaaa aatccctgct
    15721 aaaggagaag cctttgatcc taatcttcat caagcagtac aaactgttcc agcgactgaa
    15781 gatacaccag ccgatacgat tgtagaagta ctacaagaag gctacaaatt acatgatcgc
    15841 gtgttaagac caaccatggt cattgttgca caataattgt gcggatataa aacttacact
    15901 ttcaattaaa gaaggagacg ttaattatga gtaaaattat cggtattgac ttaggtacga
    15961 ctaactctgc agtagcagta ttggaaggaa acgaagcaaa aatcatcgct aacccagaag
    16021 gaaaccgtac aacaccttct gttgtatcat tcaaaaatgg agaaatccaa gtaggggaag
    16081 ttgcaaaacg tcaagctgtg acaaacccaa atacgatttc atcaatcaaa cgccatatcg
    16141 gtgaagcagg ttacaaagta gatgtagaag gtaaatcata tacaccacaa gaaatctcag
    16201 caatgatcct tcaatatatc aaaggattcg cagaagacta tttaggtgaa aaagtcgaaa
    16261 aagcagttat cactgtgcca gcttacttta atgatgcaca acgtcaagca acaaaagatg
    16321 ctggtaagat cgctggtcta gaagtagaac gtatcgtcaa tgaaccaact gcagctgctc
    16381 ttgcctatgg tttagataaa acagatcgcg acgaaaaaat cttagtattc gaccttggtg
    16441 gcggtacttt tgacgtatct atccttgaac taggagacgg tgtctttgat gtattgtcaa
    16501 cagctggcga caaccatcta ggtggggacg actttgataa caaaatcatc gactacatgg
    16561 tagcagaatt caaaaaagaa aacagcattg acttgtctca agacaaaatg gctttacaac
    16621 gcttgaaaga cgctgctgaa aaagcgaaaa aagatttatc tggcgtatca agcacacaaa
    16681 tcagcttgcc atttatcaca gcaggagaat ctggaccatt gcatttggaa atgacattga
    16741 cacgtgcaaa atttgatgaa ttaacagctg acttagtaga acgtacaaaa gtaccagtac
    16801 gtcaagcgct aaaagatgca ggtttatcac aatcagaaat cgacgaagtt attttagtcg
    16861 gtggatctac tcgtatccct gcagttgtag aagcagtacg taaagaaaca ggaaaagaac
    16921 caaacaaatc agttaaccct gatgaagtag ttgctatggg tgctgctatc caaggtggcg
    16981 tgatcactgg tgatgtgaaa gatgttgttt tacttgacgt tacaccattg tcattaggta
    17041 tcgaaacaat gggtggagtg ttcactaaat tgattgaccg caatacaacg attccaacaa
    17101 gtaaatcaca agtattctca actgcggcag ataatcaacc agcagtagac atccatgtat
    17161 tacaaggtga acgcccaatg gctgctgaca acaaaactct aggaagattc caattgacag
    17221 atattccagc agctcctcgt ggtattccac aaatcgaagt aacattcgat attgataaaa
    17281 acgggattgt aaatgtatct gcgaaagatc taggtacaca aaaagaacaa aaaatcacga
    17341 tcaaatcttc ttcaggtcta acagacgaag aaatcgaacg tatggtaaaa gatgcagaag
    17401 caaatgcaga agcagataaa gctcgtaaag aggaagttga cctacgtaat gatgtagatg
    17461 cattgctatt ctctgtggac aaaacattga aagaattaga aggcaaagtg gacgaagaag
    17521 aagtgaaaaa agcagaaaca gctcgcgatg aattgaaagc cgctgttgaa gcaaacaaca
    17581 tcgaagaaat gaaaactaaa cgcgatgcat tgaacgaaat cgttcaaaac ttgactgtta
    17641 aactatacga acaagcggca caacaacaag ctcaagaaaa cccagaagca gctcaaggcg
    17701 gagcagatga tgtcgttgat gctgattttg aagaagtcga tggcgacgat aaataatcag
    17761 ttcactgaat ttcgagagaa ggccaaagtt gctactttgg ctttttcttt tgaaaaatag
    17821 tttgaagtat cggtttttgt ttcaaacaaa atatgatatg ataacaagcg acgtgaaaat
    17881 gatgagaacc aataggagga aagcctatgg caacaaaacg tgattattat gaagtcttag
    17941 ggctctcaaa aggagcatca gaagatgaaa taaagaaagc ttatcgtaag ctttcgaaaa
    18001 aataccatcc tgacatcaat aaagaacctg atgcggaaga aaaattcaaa gaagtatcag
    18061 aagcatatga gattttaagt gacccgcaaa aacgcgctgc atatgaccaa tacgggcatg
    18121 caggcacaga tccaaattac ggtgctggtg gagctggtgg cggatttggt ggattcggcg
    18181 gcttctctgg tggcggcttc ggtggttttg aagatatctt cgactctttc tttggaggag
    18241 gcggtcggtc agtcgatcca aatgcgcctc gtcaaggtgc tgatttgcaa tatacggtcg
    18301 acttgtcttt tgaagaagcg atttttggtg tagaaaaaga aatcaagtat aatcgggaag
    18361 aaatctgtca tacttgtggt gggaacggtg caaaaccagg cacacaacca actacttgtc
    18421 ataaatgtca tggtgcaggt acgattaatg tagaacgcca aacaccactt ggacgagtga
    18481 tgagccgtca aacttgtgat gtttgtcatg ggacagggaa agaaatcaaa gaaccttgtc
    18541 caacctgtca tggctcagga catgagaaga aagcacattc agttaaagtg aatgtaccag
    18601 ctggtgtaga agaaggacaa caaatgcgtt tagctggtca aggagaagct ggagaaaacg
    18661 gcggaccatt tggtgatttg tatgtggtct tccgtgtaga agaaagtgat atctttgatc
    18721 gtgaaggttc tgaaatttac tatgaattgc ctttaagttt tgtacaagca gctttggggg
    18781 atgaagtcca agtaccaact gtccatggag atgtgaaatt gaagatccct gctggaacac
    18841 agacaggtac gaacttccgt ttacgtggaa aaggcgctcc gaagttacgt ggtggtgcaa
    18901 caggcgacca acatgtcaaa gtaaaactga tcacaccgaa aaatctaaat gaacaacaaa
    18961 gagaagcatt acgtgcattc gctgaagctg gcggcatcaa agttgaagaa cagcaagaag
    19021 acggtttctt cgataagttt aaagatgctt ttggcgggaa aaagcggaag tagtttttct
    19081 agtaaaaacg ctaagtcatt aataattaaa aaccagacga acaatcatat gttcgcctgg
    19141 tttttttgta gttgtcatgt ttgtaccaac aaaaaagcca gctttagaat atgataatcg
    19201 tcatagtttc taggggtata accagtgaga gaatggaact tgttcaatcg atattgcaag
    19261 gtgtttttat gaataaataa ttcttctgag caatttttga tgcttccgtt atttttactg
    19321 aagcatgcca aagtcttctt gaacaaatgt acttcttctt ctgtcaggct tttcaaaata
    19381 cgttttacat ataatgttcg ttgatctgac gggattgctg gtaaaacgat cccgaggtct
    19441 aaatcttcaa aaaagcggat ctcgttcaca gattgatgga aagctatcca ttcgcaaact
    19501 tgtttggcat gaaggaaaga ttgcggtgtc tcttgctctg tataacaaag cgacccaatt
    19561 ccaaagttta gctgtaactg atacatttta ttaaactttg tctggatttt atcaagcaat
    19621 gatagcaatg aggctctgga aatccgattg actaggataa tgatttgttg tctatgtaca
    19681 gtgagcaaag cctgtccgta ataagagaag accctttcga gcaactccaa taaaggattg
    19741 atccgttctt gacacaattg ctgattgtca gaaagttgtc ccacaactac ataatatgga
    19801 gaagaaaaat caagctcaat taagtgaggg tggtatcgac tatgtacttg atatcatcaa
    19861 tgaaccgatg gtactttctt atgcgatgga acaggtagag cgattgcttg tttgtgcagg
    19921 aaaacaagcg atgcggctga tacgattagg tgagaaaata agaagataaa aatacacggt
    19981 cccttcatgg cgaccgtgca tttttggttt ttattaaata tttaatactt tattcaagaa
    20041 atcttttgtc cgttcatttt gcggattttc aaagatttgt tgaggcgttc catcttctaa
    20101 aaagtttccg tcgtcaatga acatgacgcg attggccact tctttagcga accccatttc
    20161 gtgcgtaacg ataaccatcg tcataccttg ttctgctaac tttttcatga cgttcaagac
    20221 atcaccgacc atttcaggat cgagtgcaga agtaggctca tcaaaaagca tgatgtcagg
    20281 gttcatcgct agagcacgag caatagccac acgctgtttt tgtccaccag aaagcatttc
    20341 aggatacata tcttttttat cttctagacc aactgtctgc agtaatttga ttgctttttt
    20401 ctctgcttct tctctagatt ctttcttcag gtccatcggt gctaaaatga tgttttccaa
    20461 aatagaaaga tgagggaata ggttaaagtg ttggaatacc attccgatat gttgtctcac
    20521 caagttgata tctgtgcttt tatccatcaa gtgggcgccg tcaataataa tttcaccgtc
    20581 agaaggttct tctaaacgat tcaagcaacg caagaatgta ctttttcctg aacctgaagg
    20641 gccaatgata cagacgacat ctccttcgtt gatggataca ttgatgtcgt ttaatactgt
    20701 gttatcgccg tattttttga ctaaatgctc aactaagatt ttttctgcca tcttacttca
    20761 ccttcttttc taaaactttt gctaattttg tcaatgctgt gatcaaaatc aagtacatga
    20821 tcgcaatgat caagtaaaca tacgtacttt gtgttgtacg tgcaacaatg atcttaccag
    20881 tttgcagcaa ttcgactaca ccgattgcgg aaatgatcgt tgtgtctttt aatgagataa
    20941 cgaactgatt gacgaatgat gggatcatga tgcgtatcgc ttgcggcaaa atgatttttt
    21001 gcattgtccg attataagaa agacctaaac ttctggaagc ttccatttgt ccgacaggaa
    21061 cggcgttgat cccgcctcga acaatctctg cgatataagc acttgcgtta agagtcaagg
    21121 tgataacacc agcaaggaag tcaggaatcg taaatccaac gataccaggc aacccaaaga
    21181 aaataaagaa cgcaagaacc atcattggaa ttcctcggat gatatcgaca taaatactag
    21241 caattgtgcg aagagcgcgt attggtgcta cactgaataa tccaaaaatc acaccgatta
    21301 ctatagccaa agcaaaggag atcaaagtta ggagaattgt tctccaaagt cctttcaaca
    21361 atgcttgata gttgttttgg ataagtccaa gaaatgttga ttcatcagca gtggaagact
    21421 cactggaagt ttctgaacca tccgctacat attctttaat aatcttatcg tattcgcctg
    21481 ttctttttaa ttctttcaat ccttcattga acatattaag aagctcagga ttttgtcctt
    21541 ttttaacggc aaatgcatat tttccgccag attctttttt gatcggtgtt tccaattctt
    21601 gtccttgttt gatcgcataa ccgattaccg gataatcatc catcattgca tcgattgcgc
    21661 caacttttaa cccgtcgtac aattgatcag cagcatcaaa atatttgata gaatagccat
    21721 attcagattc atggtcgctt aaaaattcag cactttcggt accaactttt gctccgactg
    21781 tttttccttt caaatcttca taagacttga tcgaagtatc tccttttttg acagctaatt
    21841 ggatcccgct atcaaagtaa gaatcagaaa aatcatagct tttctttcgt tcatctgtta
    21901 cagtcattcc ggcaatcatt ccgtctgctt gtcctgattc caaagcttgc aaagcagcag
    21961 agaaaccacg gtagtccatt cggatgttaa atccttgcag tttggcgatg ttttccatca
    22021 attttacatc gatgccttcg tactcgccct ttgcgttttg aaattcaaat ggagcaaaag
    22081 cagagtcact ggcaattacg tatacgtctt ttttgggctc tattttttgc attgtctcgc
    22141 ttgtgcttga ctctgccgtt gtagtgttgt cagcttgtgc aacatgacca aacgcaagtg
    22201 tcacaagtcc tgtcattaag aaaacaaatg agtgaaataa atgctttctt ttcataatca
    22261 acctcctaaa tttccaatat tttattatta taggcgaaaa gtgctgaatc agacaagact
    22321 gtaagctcga aaaaatgaaa aaaatgcgag tttctaacat tttatgttat aaaacataac
    22381 atgtataata agataaaaaa gtggcgaaag tatgttcggt gtggtataat tttttagtaa
    22441 ggaatgcctt tgctttcctt ttctttttat tggtcgtaaa atgaaaagga atgaatgaaa
    22501 aggaggacga agaatgattg agagagatac ctcccgacac tttgagcttg tctcgaaata
    22561 ccaaccggct ggtgatcagc ctgaagcgat taaccaatta gtggatggcg tagtcggcgg
    22621 gaaaaaagca caaatcttat taggtgcgac tggaacaggg aaaacatata cgatttccaa
    22681 tcttattgag aaagtgaata aaccaacact gattattgcc cataacaaaa cattggctgg
    22741 tcagctgtac ggggaattca aggaattttt ccctaacaat gctgttgaat attttgtttc
    22801 ttattatgat tattatcagc ctgaagctta tgtgccttca agcgatacgt atatcgaaaa
    22861 agattcaagt gtaaatgatg agatcgacaa attacgacat tcggctacta gttcattatt
    22921 agaaagaaat gatgtgatcg tcattgcatc cgtttcatgt atttttggat taggctcgcc
    22981 ttttgagtac caaaaacaag tagtttcgat tcgacaggga gcagaactag atcgcaatca
    23041 gttgattcgt gacttagtca gtatccaatt cgaaagaaat gatatagatt ttcagcgcgg
    23101 tcgttttcga gtgcgcggtg atgtcgtgga gatttttccg gcttcaagag atgaacgtgc
    23161 cttacgtgtg gagttttttg gagatgaggt cgaacgtatc cgagaagtca atgcactgac
    23221 aggtgaagtt ttaggagaaa cggaacacgt agcaatcttc ccggcgactc actttgtgac
    23281 gaatgatgaa catatggagc atgccgtagc gaatatcaaa gaggaattag aacagcgttt
    23341 aactgtcttg cgaaatgaaa acaaacttct ggaagctcaa cgtttagaac agcgaaccaa
    23401 ttacgatatt gaaatgatgt tagaaatggg ctacacgtcg ggaattgaaa attattctcg
    23461 gcatatggac ggacgtaaag aaggtgaacc gccttatacg ctattggatt tctttcctga
    23521 agattttttg atcgtggcgg acgaatcgca cgtaaccatg cctcaaatca gaggaatgta
    23581 taatggtgac cgagcgagaa aacagatgct agtagattac ggtttccgtt tgccatctgc
    23641 tttagacaat cggccacttc ggttagaaga gtttgaaaaa catgtcaatc agatcattta
    23701 tgtctctgcg acgccaggac catatgaaca tgaacaaaca gataccgtta tccaacaaat
    23761 catccgtccg acagggctgc ttgacccagt gatcgaagtt cgtccaatca tgggacaaat
    23821 cgatgatcta gtaggcgaaa taaatgaacg agtcgaaaaa gaccagcggg tgtttgtaac
    23881 gacattgacg aaaaaaatgg cagaagacct cacagattat ttcaaagaat tagggatcaa
    23941 agtcaaatat ttgcatagtg atatcaaaac gctggaacga acagaaatca ttcgagatct
    24001 tcgattaggt gaatttgatg tgttagttgg aatcaatttg cttcgtgaag gattagacgt
    24061 acctgaagtt tcccttgttg ctatcttgga tgcggataaa gaaggctttt tacgtagtga
    24121 acgttcctta gtccaaacga ttggacgagc tgctcggaat gaagaaggta aagtaatcat
    24181 gtacgcagac aaagtaacag attctatgcg tctagcgatg gatgaaacgt caagacgtcg
    24241 tacgattcag cagaagtaca atgaagaaca tgggatcgtt ccaaaaacga tcattaaaga
    24301 aatccgagat ttgatttcta ttacgaaaga atcggaagat gatacaaaag aagcggtaca
    24361 agtctcgtac gaagaaatga cgaaggaaga aaaagacacg ttattgatga aattagaaaa
    24421 agaaatgaaa gatgcggcaa aagctttgga ttttgaaaca gccgcgaatg tgcgagatat
    24481 gattttagaa ttaaaagctt caaagtagga ggacaacatg gctaacgata aaattacgat
    24541 tcatggtgca cgtgcccata acttgaaaaa tatagatgta acgattccac gagataaatt
    24601 cgtggtcgtt acaggtttgt ccggttctgg aaaaagttcc ctagcatttg atacgctgta
    24661 cgctgaaggg cagcggagat acgtagagag cctttctgct tatgctcgtc agtttttagg
    24721 acaaatggat aagccggacg tagatagcat cgacggattg agtccggcga tttctattga
    24781 tcagaaaaca acaagtaaaa atcctcggtc gacagtaggt actgtgacgg aaatcaatga
    24841 ctatttacgc ttgttgtttg cacgtgtagg acatccgatt tgtcccaacg atcatatcga
    24901 gattaccagt caatcagtag atcaaatggc agatcaagta atggctttgc ctgagcgcac
    24961 aaagatccaa gtgttggcac cgatcgtggt aaagaaaaaa ggacaacata aaaaaatatt
    25021 cgaacgaatc caaaaagagg gatacgtccg cgttcgagtc gatggtgaga tctatgattt
    25081 aagtgaagtg cctgagctgg agaaaaacaa aaaacatgat atcgcgatcg tcatcgaccg
    25141 gatcattgtg aaagaaggga ttcgttctcg cctatttgat tctttagagg cggccctgcg
    25201 attagcagac ggctatgctc tggttgacgt gatcggtgaa gaagaaatgt tgtttagcga
    25261 acactatgcc tgtccttatt gcggatttac tgttggcgaa ctagaaccaa gactgttctc
    25321 attcaatgcg ccttttggag cttgtccaga ctgtgatggt ttaggtgtca aattagaagt
    25381 cgatcaagat ttagtgattc cagatagaac gaagacactt agtcaaggtg ctatcgttcc
    25441 atggaatcca atcagctctc agtattatcc tcaaatgtta gcgcaggctt gtgaaagttt
    25501 cggtatcgat ttagatacac cattcgaaga attaccggaa gagcatcagg agatcatctt
    25561 gaatgggaca gatgatcttt tccatttcca ttatgaaaat gattttggtg gcgttcgcga
    25621 tgtagaaacg actttcgaag gggtattgaa gaatatcgag cgtcgttatc atgatacgaa
    25681 cagtgatttc acgcgtgagc aaatgcgtct ttatatgaca gaattaactt gtcagacatg
    25741 taaaggttat cgattgaatc cccaagctct atctgttcaa atcaatggaa cgaatatcgg
    25801 gcaaacaaat gagttgtcta ttcaaaaagc agtagatttc ttctcaaact taacgttatc
    25861 tgaacaagag aaagtaattg ccaaaccgat tttaaaagaa gtaaatgaca gactttcatt
    25921 cttggaaaat gtgggactag actatctaac tttgagtcgc gcttccggaa cattatctgg
    25981 gggagaagcg cagcgaatca gattggctac acaaattgga tcgaatcttt ctggagtttt
    26041 gtatatttta gatgaaccat cgatcggatt gcatcaacgc gacaacgatc gactgattgg
    26101 ctcattaaag aaaatgcgtg atcttggcaa caccctcgtt gtagtcgaac atgatgaaga
    26161 tacgatgtgg gcagcagatt atttgattga cgtcggacct ggggccggag aacaaggcgg
    26221 acaaatcgtg gctgcaggca ctcctgaaga agtagctaat gatgaaaatt ctttgacagg
    26281 gaaatatctt tcagggaaaa aatcaatccc tgtaccaaaa gaacgccgta aaggtaatgg
    26341 gaaagcaatc aagatcactg gtgcttcaga aaacaatctg aaaaatatta gtgttgaatt
    26401 tccattagga gaatttgtag cagtcacagg tgtgtctggt tctggaaaga gtacattggt
    26461 caatagtatc ttaaagaaat cgttagcgca aaaattaaat aagaattctg ctaagccagg
    26521 taaattcaag acaatttccg gctacgaaag tatcgaaaag atcatcgata tcgatcaaag
    26581 cccaatcggc cggacgccga gaagtaatcc agcgacttat acaagtgtat ttgatgatat
    26641 ccgtgggtta tttgctcaaa cgaacgaggc aaaaatgcgg ggttataaga aagggcgttt
    26701 tagtttcaac gtaaaaggcg gtcgttgtga agcttgtcgc ggggatggaa ttattaagat
    26761 cgaaatgcac tttttgcctg atgtctatgt tccttgtgaa gtatgtcatg gtaaacgata
    26821 taattctgaa acattagaag tgcattacaa aggaaaaagc attgctgata ttttggaaat
    26881 gacagtagag gatgctgtag aattcttcaa gcacattcca aagattcatc gcaaactgca
    26941 aacgattgtt gatgttggct taggttatgt gacgatgggg caaccagcaa cgacattgtc
    27001 cggtggtgag gcacaacgga tgaaacttgc cagtgaattg cacaaaatct ctaatggaaa
    27061 gaatttctat atactagatg aaccaacgac aggacttcat agcgatgaca tcgcccgctt
    27121 gttgcatgta ttacaaagat tagtagatgc tggtaacaca gttttagtga ttgaacacaa
    27181 tctagatgta atcaaaacag cagattatat cattgattta ggaccagaag gtggagaagg
    27241 tggaggaacg atccttgcga ctggaacacc agaagaaatc attaacgtaa aagaaagtta
    27301 tacaggtcac tatttgaaaa aaataatggt ataaagagga tagatagctg agatagttaa
    27361 aaaaacataa aaaaatgaat ttttgcttgt tttattaata tcagtgttct agaatcatta
    27421 catggtagtt ctttctacac tgattggtat agataaaaac ttcctataag ttcaatccgt
    27481 tcaaaaaaga attgagcaat cggaggactt tcctttgatt gcttttttct ttatggtatg
    27541 atattccttg tgtagttgga aaagaaaaac aagagattat gagtaagtga gagataatga
    27601 tgaacagtat tgaaaatgaa gaatttatcc ctatattatt aggaagcgac atgaacgttt
    27661 atggtatggc acgttctttt aatgaagcat acggcaaaat ctgccaagca tatgcttcgg
    27721 atcagttagc ccctacccgc tacagtaaaa ttgtcaatgt agaagttatt ccaggatttg
    27781 ataaagaccc tgtatttatc gagacgatgc ttcgtttggc aaaagaaaga tattctgata
    27841 agagtaaaaa atatcttttg attgcttgtg gtgatggata tgcagaattg atctcccaac
    27901 ataaacaaga gctgtcagaa tattttatct gtccatatat tgattattct ttatttgaaa
    27961 ggctgatcaa taaagtaagc ttttatgaag tttgtgaaga atatgatctt ccgtatccta
    28021 aaacattgat cgtcagagaa gaaatgttag taaatggaca tttggaacag gaattgcctt
    28081 ttgaattccc agttgctttg aaacctgcaa acagtgtgga gtatctttct gtccaatttg
    28141 aaggaagaaa aaaagctttt attttagaga ctagagaaga gttcgattta attttaggcc
    28201 ggatatacga agccggttat aaaagtgaaa tgattgtaca agattttatc ccgggtgatg
    28261 atagtaatat gcgtgtgcta aatgcgtatg tagatgaaga tcatcaagtt cggatgatgt
    28321 gccttggtca tccactatta gaagatccta caccagcatc gattggtaat tacgttgtga
    28381 tcatgccaga ttataacgaa aaaatctatc aaaccattaa ggcatttttg gaaaaaatcg
    28441 aatatacagg ctttgccaat ttcgatatga agtatgatcc tagagatgga gaatataaat
    28501 tatttgaaat caatttgaga caaggtcgta gcagtttctt tgtgacattg aacggcttga
    28561 atctggctcg ttttgtcaca gaagatcgag tgttcaacaa accctttgtt gaaacgacct
    28621 atggaacgaa tcaatcagat aaagcaagat tatggatggg tgtgcccaaa aaaatctttt
    28681 tagaatatgc tcgggaaaat gaagacaaaa aactagctga gcaaatgatc aaagaaaatc
    28741 gttacggtac tactgtattt tatgaaaaag accgatcaat caagcgctgg ttgttgatga
    28801 aatatatgtt ccataactat attccaagat tcaaaaagta ttttcatgtg aaagaaggct
    28861 agttcatgga gaattttttc agtattttag gcggaatggg cacgatggcg acagaaagtt
    28921 ttgttcgctt gatcaaccac cgaacgaaag ctacaaaaga tcaagaatat ttgaattatg
    28981 tcttattcaa tcatgcaaca gttcctgatc gtacagctta tattttagat cgatcagaag
    29041 aaaatccaat gccgtttctg ctggatgata ttgagaaaca aaatttattg cgaccaaact
    29101 tcattgtgtt gacttgtaac accgcacact acttttttga agagttacaa gcggcaacgg
    29161 atattcctat cttgcatatg ccaagagaag cagcaaatga acttgtccgt cagcatacga
    29221 cagggagagt agcgattcta ggaacagaag gaagtatgaa agctggaatc tatgaacgcg
    29281 aagtcaaaaa tcttggtttc gaaacagtga ttcctgatac tgcgcttcaa gaaaaaatca
    29341 attacctgat ctatcatgag atcaaggaat cggattattt gaatcaagag ctctattacg
    29401 aaatcttaga ggaagcggta gagcgcctaa actgtgaaaa agtcatccta ggttgcacag
    29461 aattatcttt gatgcatgaa tttgctgaag ataatcatta cccagtgatc gatgctcaat
    29521 ctattttagc ggatcgaacg atcgaacgtg cattagctga aagaagcgag gcattggata
    29581 cagcatcgga aaagtaaaaa aattggattt ggggcaaata tcctaaaaga tatttggtcc
    29641 aaatcttttt gtaaaagaaa aagaaaactg gtaatgatac gcccgggatg atataatttt
    29701 attatgcaca atgtgagagg ggttcgtaaa tatgacagat aatttgcaat tagtcatcat
    29761 aacaggtatg agtggtgcag ggaaaacagt agctatccaa agttttgaag atatgggcta
    29821 tttttgtatc gataatatgc cgcctcgatt gattccaaaa ttctgggaat tgatcaaaga
    29881 atcaggtaaa gtcacaaaaa tggctttagg agtggatttg cgttctcgtt ccttctttga
    29941 agaaatccaa aacatgctga tcgaaatcga aaatacgaac ttcatcgaca cttctatctt
    30001 atttttagat gcttcagacg aagaactagt ggctcgctac aaagaaacaa gacgagcaca
    30061 tccaatggcg atggacggtt tgatcactga agggatacgt aaagaacggg cgatactaga
    30121 cgacttgaaa gcccaagctt ccgtgatcat tgatacgaca acattgacac cacgacaatt
    30181 acgtgaaaaa atcaatgagt cattcaaatc aaagggagac caaggatttc ggatcgaatt
    30241 tgtatcattt ggttttaaat acgggttgcc gatcgattcg gatattgtga tggatgtgcg
    30301 ttttttgccc aaccctcatt atatcccgga actaagacca ttgaccggaa aagatcagcc
    30361 agtctatgat tatgtgatgt cctttgatga aacgcaatca ttctaccata aatttttgga
    30421 tcttctggaa actgtattgc ctggatacat taaagaagga aaaagcagct tgacagttgc
    30481 aatcggctgt acaggcggtc aacaccgttc tgtggcattg actgaacgtt tggcaaatgc
    30541 tttagggaaa aactataaag tcaatgtgac acatcgggac aaggataaaa ggaaagagac
    30601 ggtgaaccgg tcgtgaaaat gaaaacctac cggataagaa aaccaaagat cgtagtggtc
    30661 ggcggtggca ctggtctgcc agttatcctg aaaagtttac gaaatcaagg agcagatatc
    30721 acagcagtag tgactgttgc agatgatggt ggaagcagcg gtgcaatcag agaatcgatc
    30781 gctatggctc ctcctggtga cttgagaaat gtcttagtgg cgttatcgga tatgccacaa
    30841 ttctatgaag atattttcca ataccgtttc aaaaaggaag atcagttttt agccaatcat
    30901 acgatcggta atctgatcat tgccgcagtt tccgagatgc ggggaagcac ttatgaggcg
    30961 attcaattat tagcaaaaat gatgcatgta gatggacatg tttatccttc ttcagaaact
    31021 cctttgacct tgcatgctgt ttttaaagac ggaacgactg cagtaggaga atctaaaatc
    31081 gcgatcaacc gtaaaaccat tgaccgtgtg tatgtctgca acacaaatga taaaagtgaa
    31141 gccaaagcgg ctcgaaaagt cgtttcagca attatggacg ccgacatggt cgttttggga
    31201 cccggaagtt tattcaccag tatccttcca aatttagtga taccagaaat cggcgaagca
    31261 atgctcaaga caactgctga aaccgtctat atctgtaata tcatgaccca aaaaggggaa
    31321 acggaacatt tcacagatgc cgatcatata cgtgttttac acaagcattt gaatcagcct
    31381 tttatcgata cagtattggt caatacggaa aaagtgcctg aagattatat ggacccagag
    31441 atctatgatg agtatctagt acaagtcgca cacgacttca acggattgag aaatgaagga
    31501 tgtcgagtga tttccaccga ctttttagaa ttgaaagatg gtggtgtctt ccacgatgga
    31561 gacaaagtag tagaagaatt gttccgttta gttttcggtt caaaatatta agcaatttat
    31621 tgttggagaa aggaggagaa taggatgtca tttgctgcag atgtgaagaa agaactaact
    31681 ggtttggaag ttcatcgaga acatgcgaaa gcggaattag ctgctttgct gcgaatgaac
    31741 ggctctttag gtattgtgaa ccagcagttt attttgaacg tccaaacaga aaatgctgca
    31801 attgccagac gtatctattc tcttttgaaa gatcattatc aggtacgaag tgagctactc
    31861 gttcgtaaaa aaatgaaatt aaaaaagaac aatgtctata tcgtccggtt gaagcaaggc
    31921 acaaaagatg tgttgatgga tcttgatatc atggatggta tgatgttcaa tggacacgtt
    31981 tcggacgaga ttatgggaaa ttcgcaaaaa atgcgttctt acttacgtgg tgcttttatg
    32041 gcctcaggtt cagtcaataa tccggaaacg agtcgttatc atttggaaat attttctatt
    32101 tatgaacagc ataaccaaga catttgcgat atgttaaatt attacggtct acatgctcga
    32161 acgcttgaac ggcgcaatgg ctatatttcg tatttaaaag gagcagaaaa aattgctgat
    32221 ttcttaacat tgattggtgc aaccaactca atgctccgat ttgaagatgt tcgaatcgta
    32281 cgggatatga gaaattcggt caatcgtcta gtcaattgtg agacagcaaa tctgaacaag
    32341 acgattgatg cggcttctaa acaaatcgaa aacatcgaat tgatcgaagc aaaggtcggt
    32401 ctacatgccc ttcctgaaaa acttcaggaa atcgcagaac ttcggcttca acatccagag
    32461 gtcagcttga aagaattagg ggaaatgatt ccctcaggtg caatatcaaa atcgggaatt
    32521 aatcaccgaa ttcgcaaaat aaatgaattt gcggaaaaac tgcgtgaaga agcagtttaa
    32581 tccaaagaaa aaactgatac atgaagcagg agatgcgaat gtatcagttt tttttaaaag
    32641 ctatacttgc ttttcttccg tcaaactata attccaactt tactggagta acataatgtt
    32701 gaaaggcttc tattgttttt taatacaatc gtataaaaag cggggagaca actgaatgaa
    32761 gattttagtt ttatacataa acttttgctt aatatagaag tcctgatata gataggatca
    32821 agtatttatc tataactgga gattaattaa aaactggaag gaactgctcg atcaaccttg
    32881 agaactgttt ttcttacttc tggtcaagat tctcgtggtt ggtggataag gatggttgat
    32941 aagttcattg gattagctag tcgaaaacaa ccaggtgtaa ttaatgtaga agttttaaca
    33001 aggggatgga gtgatttatt cagtgataat cataagttca taaatagtaa ttgggatgta
    33061 tggttggtat acgttatacc tgaacttgaa ctatcaagat ttggtttaga atttgagaat
    33121 cgatgtttat aaagggaggg caaaaaatgt gcttttttac aacttttact atcattatga
    33181 ttgcagtact ttttattttt ctagatatag caaagcgaaa tacagcattt ttattatatc
    33241 gggttttact tcgagcaggt ctcataacat ttatatcgat cgttggattt tttctattca
    33301 cagttattgt atttatttgg agaactacag caccaccatt gccagagatt acatatggag
    33361 aatttccgtt tagtcttgaa tatgaaatag atgaggaaag aattttgatt gaagatacgg
    33421 tgattgcaga atttatagga agttttagag ggaatgtgac aactcaacct agacgaagtt
    33481 ggagtataaa acttttaaat aatagtggag agttgaaagc atttcattat gaattcttaa
    33541 taatagaaag tgaagatgta acaatcactt taagtagtgg ctctgctgga tattttatga
    33601 atgatggtgg aagcaagcca cagctcacag tccgtgatcg tattgaaggt tttatgcatt
    33661 tttctcacta tgacttagaa gaagctcgta aaatacttgc agaatatggg atcacattaa
    33721 tcaattggga atatgcgcca ccgataagaa attttttcaa tgattattat tagtcttata
    33781 aaaaacagaa tgtaaaattt atgtgcgcga gtatttagtt ttccattttt tgtttgatag
    33841 atatatagca ttgttgaagc atgggatagt ggtgccaatt ttatcaatga ggttggtcag
    33901 gcagttaaca taattggtga tgctataaat ccatttaatt ggaattggag gaattaggtg
    33961 tggaaacagt gagattatgg atattcatga aaaattttcg ttcggctttg agatatgtta
    34021 ttctggaatc aaaatcaggt tattatctga ttgacacaga tcactatact ttttggggag
    34081 gctacctttt tccgattttt aattggataa tcccccataa ggttatgaaa attaacttgc
    34141 ctgcagaaga aataaataga ttaatactca acaaagattt acaaaggaaa agagagaaag
    34201 aacattctaa tgttgcttgg ataggtccag catttgcagt agtgacttct tcaattgtcg
    34261 gtcctatgct agagagtatt tatagtgatt tttctattgg aatacgttat ttatttgtta
    34321 ctttaagtat agctgttctt ttgtgcataa agggttatgt tagcaagaag aataaagata
    34381 ttttaacact aatcggtgta aacaatttat ctagtactaa aattatactg tttcctgaat
    34441 ctataaaaga aggatcaagg ttgttgataa tttttataat gcttatactg ttttctgttt
    34501 tatttggggt gatgtttata ga
//
LOCUS       DASHRQ010000027        34380 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.027, whole genome shotgun sequence.
ACCESSION   DASHRQ010000027 DASHRQ010000000
VERSION     DASHRQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.027"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(197..916)
                     /locus_tag="U2M88_001395"
     CDS             complement(197..916)
                     /locus_tag="U2M88_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM8373784.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(1043..2314)
                     /gene="celB"
                     /locus_tag="U2M88_001396"
     CDS             complement(1043..2314)
                     /gene="celB"
                     /locus_tag="U2M88_001396"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEM8373785.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            2467..3864
                     /locus_tag="U2M88_001397"
     CDS             2467..3864
                     /locus_tag="U2M88_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEM8373786.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            3945..4385
                     /locus_tag="U2M88_001398"
     CDS             3945..4385
                     /locus_tag="U2M88_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEM8373787.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            4533..4859
                     /locus_tag="U2M88_001399"
     CDS             4533..4859
                     /locus_tag="U2M88_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEM8373788.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            4860..5609
                     /locus_tag="U2M88_001400"
     CDS             4860..5609
                     /locus_tag="U2M88_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373789.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(5689..6144)
                     /locus_tag="U2M88_001401"
     CDS             complement(5689..6144)
                     /locus_tag="U2M88_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEM8373790.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(6571..6846)
                     /locus_tag="U2M88_001402"
     CDS             complement(6571..6846)
                     /locus_tag="U2M88_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373791.1"
                     /translation="MKLEDKKVILKKIKRLPEQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            7140..7955
                     /gene="thiD"
                     /locus_tag="U2M88_001403"
     CDS             7140..7955
                     /gene="thiD"
                     /locus_tag="U2M88_001403"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEM8373792.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(8284..9735)
                     /gene="cls"
                     /locus_tag="U2M88_001404"
     CDS             complement(8284..9735)
                     /gene="cls"
                     /locus_tag="U2M88_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEM8373793.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            10076..12742
                     /locus_tag="U2M88_001405"
     CDS             10076..12742
                     /locus_tag="U2M88_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEM8373794.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            13106..13858
                     /locus_tag="U2M88_001406"
     CDS             13106..13858
                     /locus_tag="U2M88_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM8373795.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(13880..15034)
                     /locus_tag="U2M88_001407"
     CDS             complement(13880..15034)
                     /locus_tag="U2M88_001407"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEM8373796.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            15219..17660
                     /locus_tag="U2M88_001408"
     CDS             15219..17660
                     /locus_tag="U2M88_001408"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEM8373797.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(17990..18694)
                     /locus_tag="U2M88_001409"
     CDS             complement(17990..18694)
                     /locus_tag="U2M88_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373798.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(18881..19126)
                     /locus_tag="U2M88_001410"
     CDS             complement(18881..19126)
                     /locus_tag="U2M88_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373799.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(19143..19994)
                     /locus_tag="U2M88_001411"
     CDS             complement(19143..19994)
                     /locus_tag="U2M88_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEM8373800.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            20160..21164
                     /locus_tag="U2M88_001412"
     CDS             20160..21164
                     /locus_tag="U2M88_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEM8373801.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(21405..21761)
                     /locus_tag="U2M88_001413"
     CDS             complement(21405..21761)
                     /locus_tag="U2M88_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEM8373802.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            22129..23316
                     /gene="tuf"
                     /locus_tag="U2M88_001414"
     CDS             22129..23316
                     /gene="tuf"
                     /locus_tag="U2M88_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEM8373803.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(23504..26095)
                     /gene="mprF"
                     /locus_tag="U2M88_001415"
     CDS             complement(23504..26095)
                     /gene="mprF"
                     /locus_tag="U2M88_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEM8373804.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(26121..26882)
                     /locus_tag="U2M88_001416"
     CDS             complement(26121..26882)
                     /locus_tag="U2M88_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM8373805.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            27160..27681
                     /locus_tag="U2M88_001417"
     CDS             27160..27681
                     /locus_tag="U2M88_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEM8373806.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            27740..28600
                     /locus_tag="U2M88_001418"
     CDS             27740..28600
                     /locus_tag="U2M88_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEM8373807.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            28785..31100
                     /locus_tag="U2M88_001419"
     CDS             28785..31100
                     /locus_tag="U2M88_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEM8373808.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            31282..33123
                     /locus_tag="U2M88_001420"
     CDS             31282..33123
                     /locus_tag="U2M88_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEM8373809.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            33147..34256
                     /gene="rpoD"
                     /locus_tag="U2M88_001421"
     CDS             33147..34256
                     /gene="rpoD"
                     /locus_tag="U2M88_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEM8373810.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT        11155 a         6070 c         7094 g        10061 t
ORIGIN      
        1 aaaaactagc tttaaaaagc taactttaga atcattacgg gtggtcataa gtctttttat
       61 gctgagttat tttttcattt ttgagctaat gcttaggata gaatagaatg ttgatttcac
      121 gatctcttct tgttacttga aactgcttac cactgtcacg atctcttggt aaacggcttt
      181 ttcacaacct ctttggttaa gttgaacgca catccagcac agaatagttc cgaacatcaa
      241 atcggtttcg gctggaagaa tattcaatcg gctgtccatc tttgagatag acgacttgct
      301 gaacttccag tacaggatcg ttttctgcac agttcaaata ttgctgatca tattcatcac
      361 ttttatctgc agaaatagca cgataagccc ctgcaaaatg gacattcaat tcttgatgaa
      421 gatgatcata gatagaggct aacaaatgtt ctttttttaa acccggcaca agatggactg
      481 gcataaaggt atgctctaaa acatatggct cgccatctaa gatccttaac cggtgaatat
      541 tgtagacagg ttgctcggca tcgatcatca atcgttcttg gatatattga ttagggaatt
      601 caacatcaaa atgaatgatt tgactcttca aagaacgatg tctgcgtttc atttggagac
      661 tcaaaccttc atactcgtta gcgggcgatg tgtctttttg cataaatgga tgattcaaga
      721 ttttcgttcc tgatcctcgc tgcgaataga caagtccttc cattgctaag atattgatgg
      781 cttttttgat tgtcatccgg ctcgcaccaa attcttttac caagtcgact tgattaggca
      841 aaaaactatc gagaggatac tcttttgaac ggatacgttc tcttaaaata tttgcgattt
      901 cttcgtattt tggcacgtta ttcacctctt ctagagtata acgaaagatt atgcttccga
      961 caactcgaag tagcatctgg aacattgttt attcgaaaaa agaggtcatg acaagttttt
     1021 gtcacaaccc cttaatgatc tattaagcaa tatctaaatc tggttgttta tctagtactt
     1081 tcaggaatgg gtaccagata aaggcaacga tgacaaagtc gatgacttgc agcataccac
     1141 caagccatga atttgttgct aagacaccgc ttgcaatgat tggcacagtc catggaacag
     1201 agacgccagt tggtgcaggg acaagtccag cagccattac caaataattg aacgtcgtta
     1261 cgattagagg tgccacaacc caaggaataa ggattgttgc attcaatacg atcggtagac
     1321 cgaagatcgc tggttcattt acattgaaaa taccaggtgc taaagctaat cgtccaacat
     1381 cacgatactg tttcttttta agaacaaagg ccatcaataa aacgacagct aaagtcattc
     1441 cagagccacc aataccaaca gtgaaggttt ccatgaatgg ttttgtgatg atgtgtggta
     1501 attcttttcc atgttgataa gcttctaagt tatcaagcat cagtgtattc caaatcgggt
     1561 ccattacaga gttgacgatg atttgtccgt gaagtccgaa gaaccataag aattggacaa
     1621 agaacaaagc aatcaatgtt gcaggtaaac tacttcccaa accagttaat ggtttttgga
     1681 tcacttcata aacgacgtca tgtaagttag cattgaaaat accagtcatc acagcattga
     1741 ttactaagaa aatagttagt gtcactacag cagggatcaa agcggcaaat gattttgtta
     1801 ctgcaggcgg aactccatca ggcattttga tctgccagcc gcgtttcgtg attctgcagt
     1861 agatttctcc agccaagaaa gcagcgatca ttccaatgaa cattcctttt gcgccaagac
     1921 gatcaagtga aagcacacca gtcacatctt caccgccagc ggttttcatt gcaaaaggcg
     1981 taagaatcaa gaagctggca aaagacacgg caccaccgaa aataccttca acgtcataag
     2041 attttgataa ataatagccg ataccaaaag taacaaaaac cgacatgatg ctcattgttg
     2101 cattttgccc attaccgaat aaattcgata aagttccctt tgttgcgtca ctgaaaaaag
     2161 gcaagttatt gataacgaca acgatcgaac cgaacatcgt taaagggaag gaaagcatga
     2221 aagcatcccg cagcactgta aggtaacgat tttgccctaa taaattagct aagggcatga
     2281 ttttctcaga aagcttgtcc ataaaaccgt tcatttttat tcacctcaaa tttatttgca
     2341 atcgattaca tacaaagtat aacgccaaaa attttaaatg tctagtcatt ttcataaaaa
     2401 gtattgattt attttttagg gttcgttatg attacggtat caagaaaaaa gagaggaagt
     2461 aaaaaaatga aaacctacac attcccagaa aatttttggt ggggttctgc tgctagcgga
     2521 ccgcaaacag aaggaagagt aagtgatgat ggtaaaggag aaaatatttg ggatcattgg
     2581 tatgaattag agcctgaaaa attcttcgat caagtaggac ctgaaaaaac ttcgcaagtt
     2641 tacacaaaat acaaagaaga tgttcagtta atgaaagaaa ctggccataa cacatttcgt
     2701 acatctatcc aatggagtca tttgattcca gaagggacag gtgaaatcaa tcaaaaagct
     2761 gtttcttttt atcgagatta ttttactgaa ttgctggata atggtattga accttttgtg
     2821 aacctttacc atttcgatat gccactacca ctgcaagaaa aaggcggatg gttatcaaga
     2881 gaaacggtcg acgcttatgt ttcttatgca gcagcgtgct ttgaactgtt tggagatctg
     2941 gtcaaaaaat ggtttactca taatgaacca atcgtacctg tagaaggagg ctacttgtat
     3001 caattccact atccaaatca tatcaatatg aaagaagcgg tccaagtagg ctatcatgag
     3061 aatctagcaa gcgcaaaggt aatcaaagcc taccatgaag gcggacatga tggtgagatc
     3121 ggaatcatct tgaatctgac accaagctat ccgagagatg aaacgaaccc agaagatgta
     3181 aaagcggcga agatcgctga cgcattcttc aaccgatcat tccttgaccc tgcagtgaaa
     3241 ggagagtttc cacaagagtt gatcgaaatt gtcaaagaat tggctataat gccagagatc
     3301 catgaaggag acctagaact catccgtgaa aacacgattg acctgctagg aatcaattac
     3361 tatcagcctc gtcgaatcaa agcaaaagaa actccaatca atcacgaaca tagccccatg
     3421 ccagatgatt attttgataa ttacgacatg cccaataaaa aaatgaatcc atatcgtgga
     3481 tgggaaattt acgaaaaagg aatctatgat attttaacga atacaagaga aaactatggt
     3541 aatatcaaat gctatatttc tgaaaatggg atgggggtcg aaggagaaga acgatttgtt
     3601 aacagcgaag gaattattga agatgattac cggatcgagt ttgttacgga tcatttgaaa
     3661 tacgtccatc aagcaatcca agaaggaacc aattgtgtgg gttaccacat gtggacatgt
     3721 atggacaact ggtcttggac aaatgcttat aagaatcgct atggctttat cgctgttgat
     3781 ttggcacaag acggtaaacg gacgatcaaa aaatcaggtc gctggtttaa aacggtctct
     3841 gaaaaaaacg gatttgatgc ctgatatttt ttgataaaac gttcacaaaa tgtttgttct
     3901 aacactgtaa tcgtgctaca ctaacagtga aggaggcgtt tacaatggta gatacttatc
     3961 gtaatatttt agtagcaatt gatggttctg aaaaggcaga aaaagcattt cttgaggcaa
     4021 tcacgattgc caaacgaaat caggcaacgt tgcacatcct gtatgtcaat gaagtaacag
     4081 gcaattactt tggtgatttt gcatttgtta caacaagcct tcaggaagaa ttggatgagg
     4141 ttgcggaaaa ccagatgaaa gaacatcgaa acttggcaat tgaaaaaggc ctgacagata
     4201 tcgagacata tgttctttac ggttatccaa agacactgat tgctaatttc catgaatcga
     4261 aagaaccaat cgatctgatt gtcatggggg cgacaggtct gaatgcggta gaacgtgcac
     4321 tagtaggttc cactacttcc tatgtagtca accacgcatc atgtaacgtc atggttgtaa
     4381 aataaataaa ttaagggaac agaaagcaaa aaagcataac gcttcttttg ctttctgttc
     4441 cttttatttt tttgtaaaaa attactttac ctgatgaagt aatcatgcta ttctaattac
     4501 aacgacagat ggagtaatat ggaggaaaga aaatgatttc aagtgatgga atacgcggat
     4561 acaatgatgc gattatttta tcgatcctta acaaaaacga ttcttatgga tatgaaattt
     4621 ctaaaacgat tcgtgagagg acaaatgatt cctatgtgat caaagagaca acactttact
     4681 ccgtttttgc ccgtttagaa aagaatcaac tgattgtttc gtatcctgga gaagtgactc
     4741 atggaaaaaa gcggacatat tataaaatca ctgaaatagg gcgtaatctt ttagaagaaa
     4801 aaaagcaaga gtggctgacg acgaaagacg tagtcgatca atttttggag ggaaattaaa
     4861 tggatacaat ccgtaattat ttagacagtc tttttatcgg agtacctcaa agtaccgaga
     4921 tagataagct gaaaactgat ttgttggcaa atatggaaga ccattatcat gaattgatgg
     4981 gagaaggaaa aaatgaacag gaggccatcg gtacagtaat ttcgacattt ggttcaatcg
     5041 atgaattatt agaagaacta gatgtcgaaa aaaaacatca agctgacgag acggaaacaa
     5101 acacagcttc tatttattta agcgaagcag aaaactactg gaaagaatac agagcggcaa
     5161 gtctccaagt ggcatcaggt gttctattca ttagcttatc ttttgcaagt tttcttttct
     5221 tttgttcagc aggatatgtg ttcatgggta tttcttgtct gattttcggt atagcattag
     5281 ctgtcggttt ttttattgct tctggtatga aaatcacacg tttaaatcac tttttgcacc
     5341 atcggaaaat acctgaaaaa gtattagcag aagcaaaaga aaaagaagaa gagtatcagc
     5401 gaagttttgg cttctcactg attgcaggaa ttggattatg tattttttca cttttcccac
     5461 ttttggcttc tttaatgtgg tatatggatg gtagtatcgg tgcctctata ttttttgtta
     5521 cggtaggaac gggtgtcttc ttgatcattt atggcagtct agtccgtcat tcttatcgtc
     5581 aattcactca aagcgcttat tactggtgaa tgtattcaga tttgtaaaaa tattctattt
     5641 atccggcgta gaaaaaaaga ctgtgacacg cttttgtcac agtctttttt atatataata
     5701 atttaaaaat tcatcatcta aatccgtaga attttctaga ttgcttaccc atgtaaaatc
     5761 gttatagaca gatttcagtt gacttctgcg ccattgtaca gggataaaaa gacgcatgct
     5821 atcttcattg aatccgacta ataatttctt tccgtcagta atgatgggac gttgtaacaa
     5881 agtaaaatgc gagacgatca gatcgacaag ctctgaggtc gtacattggt cgatttggtc
     5941 tttcaaattt ttataatcaa tcgagcgttt agctaataga tcgtcaaatc cgttttctgt
     6001 taatgacaat aaataaaata tctctttcct tgttaaaggc tgtttaggtg tgaaaattcg
     6061 ttctctgtaa ggcatttgat ggtctttcag ccagtttgcc atcagatggt aactatgtgt
     6121 gcgagaagaa tagatcgtaa gcataaaaaa actccttaaa tagtccatta atttgttaga
     6181 tctgttgaga taaataatag gtaaggagct gtgcataagc attgatcaaa acaacccaaa
     6241 catcattaga aataactttc taaagtctta aacttgcgac gagaaacaac tagactaata
     6301 gaaaaaatgt cttagccatt tggcttcctg tttgtttttt tgcttatgct gcagctcctc
     6361 gggaaagggt tctaacaatt tgaagaaggt tgatgattga tctagtttaa attgtgtgaa
     6421 atttgaaatc tggtgttttt actagatgaa tcacctaaca actatgatta taacttatag
     6481 aacttctgtt ttgcattcgg ttttgttata gttgttataa agttttattt tattacagaa
     6541 cattttttat taataaagaa cagttagaag ttaaaattgc tgattcacat agagtttatg
     6601 acttcttgga aattcttttt caatcagttt tttcatctgt ttatgaaatt cttttttttc
     6661 agtaaacata aaggtttggg tatcgtaacc ttcttcatgt aggaaataga ttattccttt
     6721 ttctgtgttt tgcgtataag cttttatact cctgtctttt ttgaaagaac agatcgtata
     6781 gccatctatt tgctctggta gtcgtttgat tttcttcaaa atgacttttt tatcctcaag
     6841 tttcattttt ttcacctgac tttatttttt tcatatggaa atcatagcgc ctttggttat
     6901 atgaaaacaa gaaggatact gaaatatata taaatcaaat agtggttatt tattattaga
     6961 atagcgaata caaataaacg accactattt tctgatttat tgcaaatgtt ttttcaaata
     7021 aatttgatta ccaagaaaag gtagtggtat atttactgag ggatactttg aaaaacatat
     7081 gatagaaaca gtaacagtat tatgaaaaat ctttttataa aaatggagga atcgatttga
     7141 tggaagatca gtatgtacaa gcaatgacga tcgcaggatc agatagcgat ggaagcgcag
     7201 ggatgcaagc agatatgcat acattcttta caagaaatgt ttacggtgtt tctgtcatga
     7261 ccgcatgtgt tgcaggtaat tcttacggaa tcggagcaag tgtgacattg ccaacagatt
     7321 ttatcgacaa agaatttgaa ctgattgcga aggactttca aatacgtgca gctaaaacag
     7381 gtatgctagc agattcaaag ttgattgaga cagttgtaaa aaattaccaa aaatatgatt
     7441 ttggaccatt agtcgtggat cccgtaatcg taacgaaaca tggaaatctt ttactagaag
     7501 aatctgcttt gcaaagcttg aaagaaaagc ttgtcccttt ggccgaagta ctgacaccta
     7561 atttttatga agcagaaaag ctgactggac ggacattagc agctgaagaa gattttgtgc
     7621 aagcagcaaa agaactgcaa gatatgggtg caaaaaacgt tatgatcaaa ggacatcatg
     7681 ctaaaaatgc gcagcaagaa acagtcaatg actacgtatt gttcgaatca ggaaaatcat
     7741 tttggttaaa tgcaccattt tatcctaccg accggatcaa cggaactggc gattcgctat
     7801 ctgcatgtat tgcagctgag ttagcgaaag gaaaaacagt tgaacaagcg atccgattgg
     7861 caaaagacta tgtaaaccgt gcaattcgtg aagaaataaa tgtagggcac aaatatggcc
     7921 ccatcaatca ttgggcagct ggtgaattag agtaaaagaa agaagttgtg aaaaagttgc
     7981 cctgcatcaa gtaataagaa cagccaagta aaaacagctt cttatgtttt taacttggtt
     8041 ggacttatta ccgcagatgc aagcttttga acaccgttta agaaagaggt cgtgagcctg
     8101 ccgtccagct ccgagtcaca aggaacaagt tcaaaaaaca gcttctcatg tttgttgaat
     8161 attgtgactt gtgaccgagg agttggcagg agaacaccgt ttgttcggaa gaagggactg
     8221 tgacaagatt tttgtcacag tccctttaat cttagtatat ttttctctac ttgaataaaa
     8281 aaattatagg attggagaaa ataatcgaga aatttgttgc ttgaataata gccagttaga
     8341 catttccttg actgtttcag ttgtcatgga tgaacaatgt gtctcatctt ctttgaactg
     8401 agtagcaagt tcttctaaaa actcaggatc gtaaatgaca gcacttgttt caaaattcaa
     8461 tcggtagcta cgaatatctt gatttgctga acctaccatg cagatttcat catccattat
     8521 caatgttttt gcatggagga agccgttctc atagacaagg atttgtacat tttccttgat
     8581 cagctgccga gcataatatt gtgttgctcg ataaataaaa ggatgatccg gtttgttggg
     8641 aatcatgatt ttcacatcta caccagaggc agttgcgatt tttaaagcag caatgacact
     8701 ttcatcagga acaaggtaag gtgtctgtat ccaaacacgt tttttagcag aagtaatcaa
     8761 tttgataaat gaaagcttga tttgttccct ttcgttattc ggtccgcttg aaacaagctg
     8821 gatggatgta tcagcaagct tagattcatc tcgatctgct tttttaaaga aataattcaa
     8881 atgatagccg accttttttt cttcaggtac cgagacgttc cagtctgtta agaagcgaag
     8941 ctggagcaaa gaagaagcgg caccgaaaat ccgtatatgc gtatcgcgcc aatagccgaa
     9001 tttttttgtt gtatttacat attgattggc aatattgaaa ccaccggtat aactaatctt
     9061 tccatcgatc acaacgattt ttcggtgatc atggtaattc aatcggaaac gaagtaatgc
     9121 cctttgtgag gtaacaaatg tatggacatg tccaccattt ttgattagac gattgaaatc
     9181 ttttgctttt gtgccatgag agccaaatgc atcatataat attcgaactt ccacgccttc
     9241 tgcggctttt tcttctaaaa catgtaagac ttgctggccg atattatctg tcacaaatgc
     9301 gtaatattcg atatggatcg aatgtttggc tttttttaga tcttgaagca gtgaatccaa
     9361 tttctcttgt ccgtctgtga gaagagtgac agaattcatt cttgtcagcg gcatacgatt
     9421 taatgaagag aagaagtcaa caaattgctg tttgtcttta tcgcccatgt caggatcgta
     9481 atgttcaatg gtatctcctc taaaagactg aaagttttct aattctttca agtcactttg
     9541 tcggagatag aattttttct tatccgttaa tccacgtccg aaaaataaat ataatacaaa
     9601 gcccacccca ggaagggcaa acaatactaa gagccatgcc caaatggctg ctacatctcg
     9661 gggtttgatc aaaatagcca ccaaagcaat aagtgcattt aatagataaa gggcggttat
     9721 aatactagat accacaaaaa tgttcctcct tacatacaat aacattagta tagcatgata
     9781 gaagaaagaa gcctaccaag gtgaatatac ttttcactcc tttcatgaaa atgtaggtaa
     9841 atcgggctaa agtagttgct ttttgtaggt taataaggta ggatattagt acagttgaat
     9901 atctccttag gggagtaacc ggcaacgttt gttgcgataa tatcaacagt aagttaacca
     9961 aatggttttc tggtattgta tgaaatggtg agacctaaga caaatgcgta tttgtcttag
    10021 gtctcttttt tttcagacag gactagggcc aagatcaaaa aatgggggat gaaatttgga
    10081 aaacaaggaa caagcacaac aagcggtcta taaagaaacg atcgactcgt tgatgcagca
    10141 aacgcagagc tcaaccgatg gattgagcac cacgcaagcg aaggaaaaat tagaaagaga
    10201 tggcttgaac cagctaaaag aaaagccgaa gaagccaact tggaaattgt tcttagaaac
    10261 tttcaaagat gcaatggtta tcgtattgct gattgtcgca ctgattcaga tggtcatggg
    10321 tgactacatc gaattcttag ttatttttgc agtattgatg ttgaattcta taatcagtgt
    10381 tgtccaaaca aaaaaagcag aaggttcttt agaagcgttg aaaaaccttt cagcgcccga
    10441 tgctcgagtg attcgtgatg gtaaggaaaa aacgattccg gcaaacgaac ttgtggtagg
    10501 agatatcgtg ttgttagacg caggcgatta tgtacctgct gatggacgtt tactagatgc
    10561 gggatcatta aaagtagatg aaggtatgct gacaggagag tcgacttctg cagaaaaaga
    10621 agtaacggat atcacaagta cggtaccaat cggagatcgt acaaatatgg tattcagtgg
    10681 aacgatcgta acttacggac gcggtaaatt cttagtgact tcaactggga ataatacgga
    10741 gatcggacaa gttgctggcc ttttggaatc aacaactgag caagtcactc cgttacaacg
    10801 aggtctggat aaattcagta aaaaactaag tttggctatt ttggcattgt ctttattgat
    10861 tttaggtatc caactgtttc gtatttatct tggagaaggt acaggagata tgactgcaga
    10921 tatcgtcagc gcagtgatgt ttgccgtagc cgtagccgta gctgctattc cagaagcatt
    10981 acagtctatc gtaacaatcg tgttatcttt gggaacgaat aaaatggcaa aacgtcatgc
    11041 cattattcgg aagcttccag cagttgaaac acttggttcg acaagtatca tctgtacaga
    11101 taagactggt acattgacac aaaacaaaat gacagttgtt gactactttt taggtaatgg
    11161 cgatactggg gatttcacaa atgacccttc aaaatggaca gctgacgaac gtcgcttgat
    11221 cgaaatcagt gttttagcga atgatgcatc gatcagtgaa gacggtacta aattgggcga
    11281 ccctacggaa gtagcgttta tcgactttag tgaaaaatta aaccagcctt atcaggaaat
    11341 ccgaaacaac tacccaagac aagcagaact tccttttgac tcagatcgaa aattaatgtc
    11401 tactggacac acaattgata acaaaaatta cttgtttgtt aaaggcggac cagatatcgt
    11461 cttccaacgc agtaaaaaag tattgctaaa tggtgaagtc gttccgatga cggatgaatt
    11521 gatgaaaaaa ttccaagagc aaaatgaagc tttttcaaca cgcgcattac gtgtactagc
    11581 ttttgcttac cgtccacttg ataattttga tttatcattt gaagatgaag acgatttgat
    11641 ccttgttggt ttgcttgcta tgatcgatcc tccacgtgct gaagtgactc aggcggtaac
    11701 agaagcaaga agtgcaggaa tcaaaacaat catgattact ggtgaccaca aaacaacagc
    11761 tgttgctatc gcaaaacaaa tcgggatttt tgaagaagga aacctggctt tgaccgggac
    11821 ggaactagat gaattgacag aagatcaact aaacgaagtt ttagaaaaag tcactgttta
    11881 tgcacgtgtt tcacctgaga ataaaattcg tatcgttcgt gcttggcaaa ataaaggtca
    11941 tatctcagct atgacaggtg atggtgtaaa tgacgcacca gcattgaaac aagcagacat
    12001 cggaattgct atgggaacgg gtacagatgt ggcaaaagat gcagcagcga tggtattgac
    12061 ggatgataac tttgcttcta ttgtcagcgc agtagaagta ggacgtaatg tgtttgataa
    12121 tatcaagaaa gctatttctt atctatttgc aggaaattta ggtgcgatca ttgcgatcat
    12181 ctttgcctta gttgctgact ggtccaatcc atttacagca ttacaactgt tgttcatcaa
    12241 tttagtcaat gattcactgc cagcaattgc attaggtaca gaaaaagctg aacctgctac
    12301 gatgaaccgc ccgccaagag atccaaattc aggaatcttt acggacaaaa cattgatttc
    12361 tgtaacgtat cgtggtgtgc tgattgggat tgctgttatc tttgctcaat ggcttggcat
    12421 gcagcattca cctgaattcg gtgtagccat gtcattttca acattgattt ggtgtcggac
    12481 attgcaaaca ttcccagcac gttcaaacac acaaacagct atccaagcag ggttcttctc
    12541 taacagaagt gtcttactag ctgtagttgt atgtagcgcg ctttactgct taacaatgat
    12601 cccaggactg aaagaagtct tctctatccc atcagcattt ggattgacag aagtctttac
    12661 atgtatggga atcgcacttg tgacaattgt tttgatggaa atcactaaat tgatcattgt
    12721 acgagtacaa ggaaataaat aaaacgaatc aacgaggttg tgagcctgcc gtttaattct
    12781 gagtcacaag gtgcaatttc aacaaacagc ttctcatgtt tgttgaatat tgtgacttgt
    12841 gaccgaagag ttggcaggag aacaccgttt aaggaagaag ttgtaaaatc agcattttga
    12901 gctaatactg agggtcatct gattggacac tgtttattcg gaaagaggga ctttgacacg
    12961 actttggtca cagtcccttt ttttatttat gatagacgga aagccaatca ttttattatt
    13021 catcaaaaga gatagaatag ataaaagagg agaatgattt attccgaaaa gtgaaaacat
    13081 ataacaaaaa attagaggaa atcaaatgaa tgaaaaagaa tgggatgaat ttgctgaaga
    13141 atattatgaa atccagcaag aatcgcaagt aactattgct gaagatgtaa aaaattatct
    13201 gaaaacggag aaagtcctgc ctgctgaaac agcagcagac gttgctggag gatctggcag
    13261 atttctttcc ttagcaaaag aaattaatcg ctatgatttg atcgattttt cttcaggaat
    13321 gctagaaaat gcaaaaaaag aagcagaaaa aaatgagttg ccaaatgttt cctttatcaa
    13381 acagtcgtta gaagaattta tccaatcaaa tgagacatat gaaatggttt ttacagcagc
    13441 aaatccagca ttggtacatc ttattcaatt agaaaaattg atgaaaaagt caagcaaatg
    13501 gtgcgtgata atcagagtag tggaatcaat cgatgatgtt ttttatccta tcgaaaagca
    13561 actaggaatg atagaagaag atcctaatac atcgccagtt gtaatggatc aatttgaaac
    13621 atacttaatt gatgaaggat tcaaagtgaa gaaaaaagat tttacttatt ttacacagga
    13681 atcaattact gaagaattga tgaagctata ctatgacgcg tacgcaaaag atcctgttgt
    13741 gaccaggtac attcgcaaac tattcagtga aaaacgagaa atccttagta cgactaagct
    13801 gacctataga ctgctccttt ggtccgcaga taaaaaaaga acctttcacg actcgtgaag
    13861 ggttcttttt atgattttat tatattttgt agtaacgaat atcattttcg atctttgtaa
    13921 tcgaaaaagg tacatctgaa gtatattctg cacattctcc gtgttcagga agcgtctcac
    13981 tgaaaatcgt ttcatattcg tcaacagtga tccgtgttcg agaatctagc atctcttgat
    14041 gagattgtgc gaagagatat tcttgatagt tttcttccag ataacctgta aaaaattcac
    14101 ttactgcacc agaaccgtag ctgaatagac cgatgcgatc tcctggctgt agtgatttcg
    14161 aattttccag tagagaagtt agccccaggt ataatgaacc agtgtaaaga ttaccgattc
    14221 gtcggctgta acggatgctt tcttcatagc gagccataag acgttcctga ttttcttcgt
    14281 ctgtttggtc taatacgctt tgcaatgcct tttttcccat cttagtatac ggaatatgga
    14341 aagcaatcgc ctgataatct tcgagtcctc gacccgacaa ttctttatgt cgattccaaa
    14401 ctttttggaa tgattcgata tacgtagaat tagataaagg accatcaaca acaggaaatt
    14461 cgctataatc tggacgccag aaatcataga tatcttctgt cagaaatacg ctgtcgtctt
    14521 caatcgataa aatacgcggg ttttgagtga tcatcatcgc aacagcaccg acaccttgcg
    14581 tcacttcacc accgcttgcc aagccgtaac gagcaatatc acttgctatg actagtactt
    14641 ttcgttctgg atgatttttg acatattctt tcgccatgtg caaagctgcg gtgccaccgt
    14701 agcaagcctc ttttacttca aaagaacgag caaaagattg gattttcagt aaatggtgga
    14761 taattaccgc actcgctttt gaatgatcaa tcccggattc tgttccgacg atgaccatgt
    14821 cgattagctc acggtctttt tctgttacga ttttgctggc agcatttgct cctagtgtaa
    14881 tgatgtcttc atttgcacga ttgactgcca tttggtcttg accaatccct atatggtatt
    14941 ttgcaggatc atccccacgg ctttctgcca actctgtcat atctaaatat aaattaggaa
    15001 taaaaaagga aagacgatca atccctattt tcatttcaac acctctttac ttatgataaa
    15061 aaacttactt tactaaggta tcataaaaag attaaaagaa atataaaaaa gagaatattt
    15121 aaggattgtt aaaatttaat cttagaaaaa aataactgga acaagagcta tttgttttta
    15181 cagcaaagaa tggtatattt ttgtaggagg tggcttactt gaaagaagtc gttatgatag
    15241 atgctgcaag aacgccgata ggaaagtatc gcggaagttt aagtccattt actgcagttg
    15301 aattaggcac acttgtgact aaaggattat tagataaaac aaaactgaaa aaagataaaa
    15361 tcgatcaagt aatatttgga aatgtcctgc aagctggtaa tggtcaaaat gttgccagac
    15421 agattgcttt gaacagcggt ctccctgtag atgtcccagc aatgacgatc aatgaagttt
    15481 gcggatctgg tatgaaagca gtgatcttag cacgccagct gatccaatta ggtgaagcag
    15541 aacttgtgat agccggcgga acagagagta tgtctcaagc acctatgctg aaacagtatc
    15601 agtcagaaac aaatgaatat ggtgaaccaa tttccagtat ggtcaacgac ggattgactg
    15661 acgcattttc aaatgcacat atgggattaa ccgcagagaa tgttgcaaca caattttctg
    15721 tgagcagaga agagcaggat cgctatgcct tgtcgtccca gttgaaagca gcacatgctg
    15781 tcgaagccgg tgtattttct gaggagatca tcccagtcaa gatttctgat gaagacgtgt
    15841 tatctgagga tgaagcagtt cgtggaaata gtacattgga aaaactgggc acgttacgta
    15901 cagtattctc agaagaagga actgtaacag caggaaatgc ttccccgttg aatgacggtg
    15961 cctctgtggt gatccttgca tccaaagaat acgcagaaaa taataatctg ccttatttag
    16021 caaccatcaa agaagtagcg gaggtcggta ttgacccaag tatcatggga atcgctccaa
    16081 tcaaagccat tcaaaaattg acagatagat caggaatgaa tttatcaaca atcgatcttt
    16141 ttgagatcaa tgaagcattt gcagcttctt ctatcgtagt tagccaagaa ctgcagttag
    16201 atgaagaaaa ggtcaatata tatggaggag caatcgcatt aggccatcca attggtgcaa
    16261 gcggtgcacg tatcttgact acattagctt acggattgct tcgtgaacag aagagatacg
    16321 gtattgcttc attatgtatt ggtggaggtt taggcttggc agtcttgtta gaagcgaata
    16381 tggagcaaac acataaggat gtgcaaaaaa aaaagtttta tcagcttacg ccatctgaac
    16441 ggcgcagtca attaatagaa aaaaatgtat tgacacaaga aactgcgttg atttttcagg
    16501 aacaaacact ttctgaagag ctctccgatc acatgatcga aaaccaagtg agtgaagtgg
    16561 aaatcccaat ggggatcgcg cagaattttc aaatcaatgg caagaaaaaa tggattccga
    16621 tggcaacaga ggaaccatct gtcattgcag ctgcaagtaa tggtgcaaaa atctgtggaa
    16681 atatctgcgc agaaacacct cagcggctga tgcgagggca aatcgttcta tcaggaaaat
    16741 cggagtatca agcagtgatc aatgcagtta atcatcgaaa agaagagctg attctttgtg
    16801 caaatgaaag ttatccttca atcgtgaaac gcgggggcgg tgttcaagat atctctactc
    16861 gggaattcat ggggagtttc catgcatact tatctattga ttttttagtg gatgtaaaag
    16921 atgcaatggg cgctaacatg atcaactcga ttcttgagag tgtggcaaac aagttacggg
    16981 agtggttccc tgaagaagag attctattca gtattttaag taattttgct acagaatcat
    17041 tggcttctgc ctgttgtgaa atcccattcg aacgcttagg gagaaacaaa gaaatcggtg
    17101 aacaaattgc caaaaaaatc caacaggcag gggaatatgc gaagcttgac ccttaccgtg
    17161 cagcaacgca caacaaaggg atcatgaatg gtatagaagc tgtagtggca gctacaggaa
    17221 atgatacacg tgcggttagc gcttcgatcc acgcctacgc ggcaagaaat ggattatacc
    17281 aaggattaac agactggcag atcaaagggg ataaactagt tggaaaacta acggtgcctt
    17341 tggctgttgc cacagtaggc ggagcttcca atatcctgcc aaaagcaaaa gcatctttag
    17401 ctatgttaga tattgattca gccaaggaac tagcacaagt aattgctgca gtagggttag
    17461 ctcaaaacct ggctgctttg cgtgcattag ttacagaagg aatccaaaaa ggccacatgg
    17521 gacttcaagc tcgttcatta gcgatctcaa ttggggctat tggtgaggaa atcgagcaag
    17581 ttgcgaaaaa attacgtgaa gcagaaaaga tgaaccagca gacagctata caaatattgg
    17641 aaaaaatccg ggaaaaataa aaaagcaata tctttagaag aataaagcaa gatttacagt
    17701 tattttattt actgcagatc ttgctttttt ctctatcaga aaatatgttt attattagaa
    17761 ttttttatag ttaaggttag gcatcttaag aaatcaggca actccaagat atcttcaggt
    17821 agaagagaag tcgcctaaca ttgtaggatc ataggtgatt tgttggacag gtcaagctga
    17881 tatttgagcc tgatgttaga aggaatttct ttttacttgc tgtagaaatt attatatgac
    17941 taaaaaggct gggattttaa atcccagcct tttatgacaa atagaatttt tatcgatcag
    18001 taatcgctgt cgctgcaaat tgttcgatca aaggaatcaa agtcaatttg actttgtatt
    18061 tccgttcgag tgctttttcg ataacgaact cattttcagt agtcatagaa atagttgcta
    18121 atttacgagg ttcatcagca tttttttcat taataactac ttcatgttca ttttgttctg
    18181 tgtaaaattt aggatactca gcaacggctt ttgaaaaatc agcaaaactt aaagcggcat
    18241 tcgtatcagt tgagcgtgta ttttcagttg tgtccgcttt tggtttttcc gttttttctt
    18301 ctggcacact gactgtttct tctgtaactg gtgtcgcaac agcttgcgca gttggttcat
    18361 cgtttttcaa tcgttgatca ttccagtaag acacgatttc aataagaaga ttgtcacgga
    18421 atcgttcgaa aatcccgttt tctcctgaac gaattctctt taatacagta ttgaagttgt
    18481 catcgattgg cacgttgatc cgtgtatctt catttgtttt cccagctaat ccgatcgtta
    18541 agatttgacg gtttttggga tcaagcatga cttgtaacgg caaaccataa cgtcgcaagc
    18601 tctcttcttc tagagaacgg aataattgtc ctcttacacc atccaatgaa ggacggaatt
    18661 gatttaataa tttttgtttc tgttttttct tcatatgttc tctccttcgc tcataccctt
    18721 tcgattataa gaaaaaaaca gccaaatgaa aaggagtaat gcaaaaaaac ttgcgtatac
    18781 gcaaattaca acagaaaaat ttgcgtattt tcccgttttt tgaaagaaaa ataaaaaatg
    18841 gaacggatta tgtatatata cataatccgt tccattcttt ctattttttc ggtatcaagg
    18901 caaaaacagg tagttcaggt gaataaatga cttcatgttc gtgccagggt ttgaccatta
    18961 gcgaatgatc gaaccgtttt ttcagttctt cgatgatttg atcatgtgtc cagtttcttg
    19021 caggaaaatg ttggattttc tctgcatcga tcaaaaagac aaaatcggaa tgaaaagtct
    19081 gggtaacttc ttccgagaga gaattcaagc gttcgattgg atccataaaa tcacctttcg
    19141 acttaaaatt gggtagtatc tgggtctttt gctttgccga ctacaccatc taactgatcg
    19201 atttgtttca tttcttcttc tgttaattca aaatcaaata attcagtatt ttctttgatt
    19261 ctgcttggtg tcactgactt aggaagagga aggaattcgt gttgcagcga ccaacgcaaa
    19321 gcaacttgtg caatcgtctt gtcatgtgct tcagcgattt gtttcatttc aggcacatcg
    19381 aagattttac cagtgcctaa tggactatat gcttcgagga tcatatcatg tttttttgca
    19441 tactcaacta cagttgtctg taattcgcct ggtgcaagga aaacttgatt gaccatcggc
    19501 ataatttttg ctgtttttgc tagtgtatct agatggtggg gtaagaaatt gctcacaccg
    19561 attgctttga ttttacctgc ttcatacagt tcttcaaatg ctcgccatgt atctgcattt
    19621 gcttgttccc aattatcacg aaaagcaaca gggtttggcc agtggatcaa aaacagatct
    19681 aaataatctg tttggagctt cttcaatgat tcttcaaaag aagacatcac aagttcatag
    19741 ctgtggtttg cattccataa ttttgttgtc aagaatatct cttctctagg aattccactt
    19801 tccttaatcg cttggccgac gctttcttca ttcttgtatc cttgggcagt atcgatatgg
    19861 cgataacctg cagccagcgc ttctttaaca gaggaaacag cgacatcccc atcaggtgtt
    19921 tgccatgtgc cgaatcctac taccgggatt tcgtaaccat ttgatagacg ataagtgctt
    19981 gttaatgaat tcatactttc agctcctttt ttattcgttc aactataagt gtagcgcatt
    20041 tttcgaaaaa aagaatccat tttgtttcta ctcttgaaat ttttgtttaa aagaaaatga
    20101 ttttgattct ggtatttttg attttttttc cgtaaactaa gacagaaggg aatgagaaaa
    20161 tggatatcta tttaaaaaaa gcaattctgc atattattga cagagaaact ggttctccag
    20221 tcttttcaca aaaagagctg gatctgacaa aagaatatat tcgtgatttt cttcaaaaga
    20281 agatccaaaa aatctcttct gctcaaacaa aaactggaca actagcagaa gaaagcatga
    20341 tcgttgaatc acttaaaatc gctcaagaaa attttgtgga agcaagtgaa gcaatcgttc
    20401 agcggtggtt cgatatctac caagaaagtg aagaagcacc tagtgcagat gtatttgttg
    20461 tcttgtatga attagataca gtgatgtact tatctttatt gaaagtcaat tatcgtgagg
    20521 cttacactca ctttgttgaa gcagacgaag cagggatcga caaccgcttg attttgaatc
    20581 gagcaattct aggggcgaaa acacaaaaag cagatgaagc attcgctgtg aatttgactg
    20641 attggaccta cgaattaatt gaaaaaaaat atgagttttc tggcaaaaaa gagctttatt
    20701 tttctggaag agtgatcgaa agtgttcctg caccttcttt agaagaaaat gtaaaagtga
    20761 tccaaaaagt agccaagcat cttggaaaaa aatttgagac agaagaattt gatatcatgg
    20821 ctgatttgaa agaagctgtc tatgatacga tagaagaaaa aggtcggttg gatcatgaga
    20881 tgattgcgga aaaagtcttc aaggaaaaca tcacggcaaa aatggctttt aaagaagaag
    20941 tacaagagca aggatttgtc ccagaagcac ctcctgtaaa agaagtacaa gaaatttctg
    21001 agaaaaaatt tggaaaacaa aaattcaagc tatcaaacgg gatcgaactg atcgtaccag
    21061 tagatgtata tcgcaaccca gatttgattg aatttgtgaa taatccggat ggtacgatat
    21121 ccgtgatgat caaaaatgtg gatgaagtat tgaatcgttt ataaaaatga ctagaatatt
    21181 aggtgttcaa gtaccagtga tcagttaaat gaattgtgtc aagagaagga tcggaaaaga
    21241 cgaatataac aaaaaaagag cgtgagacaa aagtaaagaa cacttttgtc tcacgctcta
    21301 aacacgtata aacggcggaa gcagaagcaa ctccttctta tttcttggag ttaaacgctt
    21361 ctgtcccagc ctctttttat ctaataaaat tttaagacca tttactaatt atcgattttt
    21421 tctgccgcat ttttaggcac ttcagaagcc tttatttctt cccagcttaa tacaccttta
    21481 cgaggattga ctttgagttt aagatacgca ttcatcttta atggatgttc tctttcttcg
    21541 cgaagcgtca cctgtttttt ttcaccatct ttattataag ccgcttgctt gtaggtataa
    21601 gtcgtgaatt tctctccgtt atctgcttgg gtagctgaca tttcgccatt cgtcgtgatt
    21661 tttgtataat aagcttcgcc gccgtaaaaa taatgataag ctgcaaaact tccgccgaga
    21721 aatatgccta aaacgacaag tacgccaatt aattttttca tgtacttcac ctctttcctt
    21781 attattaacg aaatagaaga tttcgtctat tgaaagttgt tggcaacagc tgaattttaa
    21841 acttttcctc attttgttct tattgcttca aaagaaagtt aaggactatc ttgaagtttt
    21901 tctcaaaaaa cacatttttt tatttataaa aggtactttt ttcgcgtata agacagacga
    21961 gtgctcttag ttgactttga ccaatattga cgtataataa gcttagttcg aatcttacaa
    22021 aaagtaagtc ggacgtattc tttttgattt agtcaaaaaa agactataat aaaaaggtga
    22081 gagctgaaat ttttcagtga tctatattat ttaggaggtt tgtaaattat ggcaaaagaa
    22141 cattatgaca gaagtaaacc acacgttaac attggtacga ttggacacgt tgaccatggg
    22201 aaaactactt taactgctgc gatcactaca gtattaggca aaaaaggcct agcaaatcct
    22261 caagactatg ctagtattga tgcagctcca gaagaacgtg aacgtgggat tacgatcaat
    22321 accgcacacg tagaatatga aacagaaaaa cgccactacg cgcatattga tgcaccagga
    22381 cacgcggact atgtgaaaaa catgattaca ggggcagcac aaatggatgg tgcaatctta
    22441 gttgtttctg caactgacgg tccgatgcct caaacacgtg aacacatttt attgtcacgc
    22501 caagttggtg taaaatacct gattgttttc ttgaacaaag ttgatttagt cgatgatgaa
    22561 gaattgatcg atttggtaga aatggaagtt cgcgagttat tgagcgaata tggtttccca
    22621 ggcgatgaca ctcctgtgat caaaggttcc gcattaaaag cattgcaagg cgatccagat
    22681 gctgaagctg ctattatgga attgatggat acagtagatg aatatatccc aacaccagaa
    22741 cgtgatacag ataaaccatt actattgcca gtggaagacg tcttctcaat tacaggtcga
    22801 ggaactgttg cctcaggtcg tattgatcgt ggtgctgttc gtgtcggtga tgaggtagag
    22861 atcgtaggga tcaaacctga aacacaaaaa gcagttgtaa caggtgtaga aatgttccgt
    22921 aaaacgttag attacgggga agctggggat aacgtaggcg tgttgttacg ggggatccaa
    22981 cgtgacgata tcgaacgtgg acaagtactt gctaaaccag gttccattac tccacataca
    23041 aaattcaaag cagaagtgta cgtgttgaca aaagaagaag gtggacgtca tactccattc
    23101 ttcaacaact atcgtccaca gttctacttc cgcacaactg atgttacagg aacaatcaca
    23161 ttgccagaag atacagaaat ggtcatgcct ggtgacaacg tgacgattga tgttgacttg
    23221 atccatccaa tcgctgttga aaacggaacg acattctcaa ttcgtgaagg cggacggaca
    23281 gtaggttcag gtatcgttac agagatcgaa gcataagaaa cgcgactcga atattttggt
    23341 aatgaaaaat gtttaaaata aaagagcggt ccaaacagga tttctgtttg gaccgctctt
    23401 tgttcttcaa ttgtataaga agagattgta gaagcatgaa ctttcgtttt acttcagtct
    23461 cttttattca tcatgagagt gttcatctat cggtatgcag tttttaatac tttcttcgta
    23521 tccatttttt caaaccactt attctacctt gtgtctgctc aactggcgca ttatcaataa
    23581 tcagcaaagc aatcataaca tatgcaatcc aactatctct agaatataga gtataacgtg
    23641 agacccagtt tgtagcgtac ttgcttttat attctcttaa tccatggaaa gaataaaaat
    23701 gagaaccaaa ctcgtaaacc aagtatgcaa tcctttcctg taaaaaactc tttctggaag
    23761 ttccgacatt tgaaagaggg gccatcccta aattgaacca ttggatatgt tcttctttca
    23821 tataggcaaa caaatgcaag aacaagaaat ccatactgcc agaaggtgct tttttagaat
    23881 catatcgcat cagatcaatc gttcctactt tattgttcgt gtaagtaggc atgatcgttg
    23941 caaaagcgac aatttcttct tgggtatttt tgacaactgc aataggcgct ctttgtagat
    24001 aatcttcaga gaaaaagcct aaagaaaacc ctttttcctt tcgtgaaccc aaccaattat
    24061 cagagatatt tttgaaagta ctcatttgct cagtagaaaa tggtggttgt aagacatcaa
    24121 atgtaaatcc ttctcgttcg atccgattca agacagcacg agtgcccttc atttttttcc
    24181 ctgaagttgt aaaactattc aggtctacat aagcttcttc ccccatctta atgaaatcgt
    24241 agccgaattc atgcaaaatc ataacaatct cttcgcttgt ttcgtagaaa actggtaaat
    24301 agcatagtcg atctgtttct tcgataaaag cttcgattgc ttctggaaaa tcttctttct
    24361 taccagaagg atcacccatt acaatacatt tattattgaa gcaagcgaat tggagaagaa
    24421 cagttggttc accgtctttt tcataggcaa acatccgctt atcttttaag aagatcaatt
    24481 gactatcgga gtttcctcca taagttgtaa ggatcttcag ggccttttct tcattaaagg
    24541 cttcgccaat ctgtttcttt tctccttgaa ggaaatgaac aaatagaacg atcatgaaac
    24601 taacagcgat gatggccaac aatcccgaaa accaaatctt ttcagaagga aataagaaaa
    24661 aggaaataaa atggtgacgc cgatgaggga aatccggcag attgtatacc ccaataacga
    24721 tgtaaagcaa ggacagtgca ccaataatga ttccatcgat cgtacgccac tcccatgaat
    24781 agatcaattg ttcccgaaat aattcgtttt tagaagcaag aatgatcaga agcagaatag
    24841 acaagaaaac caccggtgtg aaactaaaat cacttaataa aacataaagt aaagctagaa
    24901 caatcaagaa gattgtaggc aaatatgctc gtttcacacg tgctgcgatc cctctgccca
    24961 taatgattaa aagaaatccc aatagtatac taggaaattg gataataaaa tgaaatttca
    25021 atgggttcaa actatgaagc cagcgaaact ctgtaaaagc ttgcgggata gtggcagaaa
    25081 gtactagcat gattccagaa aagtataata agacaaccac gattttatgc gcaatttctg
    25141 ttgctaattg tttagggata ccagaataac gctgatcaaa agtggaaccg atatttttga
    25201 agaaaaagac gattccaatc aaaaacggaa tgatgtagta gaacaggcga tagagcaaga
    25261 tccacacaac gactatttct cttggcacac caatagcgga caacccgata atcatcatta
    25321 catcgaaact cccaatttct cctggaatca tagacacgat tcctatcact gaagcagcaa
    25381 tgaataatgg aattgtttgc cacagatcaa tccggatttc cattaaataa ccaacagaaa
    25441 ggaaactaaa caggacaccg gtccattcca acaatgaaac tagcaatagc tgtccttgag
    25501 ttttaagtga ggtaccacca aggaatcctt tctttttgaa agtagtaaat aaaaagacga
    25561 caggaaaata tagaccgccg ccgatcaacc atatccaata ttgcttcaaa aaaggagcaa
    25621 ctggcgtgaa gactacaagt aaaaaagaaa gcagactgta aatagaaagt ccagccatca
    25681 aaaacaagaa aactttcgat aatgcttgga tcacgtttct gctctctttc ttttgtccat
    25741 atagttcaga gcgaagcccc acactaacaa acccgccgaa accggcaata ttgttgatcg
    25801 tattgatcag ccagcttgtc tcaaacaggt aacgaggatt ctgcttttgt tcaagcatcc
    25861 gattcaaaat aatatcataa ccaaccatag gtaacacaga gataagacca atcaatagca
    25921 ttagtccgct tttccataca ggtatagtgg caaacgtttc tcctaattgc tggatagata
    25981 gagttttacc gatggacatc aattcaccga caataatgac aatgacagaa atcaaaaaga
    26041 ttgttttgaa cagtcctagg tggtttttca gccattgtat ggtatttttt aacaaataaa
    26101 tcaccttcta ttcctaatag ttatgtttca gaaagaaatt gaagaacttc tcgattgaag
    26161 cgttcaggat cttttctagc taattcatgt ccttgttctt tcacaagcac aaaggaagct
    26221 tttggtatag ttttagctat atacaaagaa tgttttagct tgattacatc tttttttccc
    26281 acgatgatca aagtggggga gttgattttt tttaaatcat tttctgtaag cccgatgtca
    26341 tgcagcaaaa gtttgataac taaaagattt ttgcgaagac ttgggcgaaa taaagagagt
    26401 aaccataccc atgcataatg aatattactg atgactctgg cagaaaaacg aacgccttta
    26461 acaagcgtgt ttccagaatt taaaatcaat cgatgaactt ttttagggaa gctgctggca
    26521 aataccagtg ctagatttgc tccatcacta aatccaagaa aatctgcctg atcgattttt
    26581 tccagcaaca tgatcgtatt cagatcctcg gccattaact gaaaattcaa catagatgct
    26641 tcgtttgtcg aacgaccatg tcctcggcta tctactagat acacggtata gtagcgttcg
    26701 agtacaggga cctgttctga aaaaaagcgg ccgctgccgt cattgccatg cagtaaaaag
    26761 agagggaagc cttgaccgct cttttcatag taaatttcgc tcccatctgg catttttgca
    26821 tatttctttt cttttttcac ataatcacct actgtgtaaa gtataacgat tatatcagtc
    26881 attgggcaat tgtttttact gaaaaacaac gatcattttc gtaagatgtc aaaaaataca
    26941 ggaaaattca ttttttctta aggcgtacac agaaatcaag gaagaaatag ccaagaagat
    27001 aaagaaatag taagataaaa aaagatgtca aaataactag gtgatgaaat gaagcgaaaa
    27061 cgattatcta ttatccgtat tcttttttac atgatcattc tgacggttat tggtgcggtg
    27121 ttgtttttat gaggaagaac ttatcgttcg gtaaaacgag tggaaagcta tcaaatagaa
    27181 ataacaacag ctgcaaaaaa ttatcatatg gaaaactacg agaatttgat caaggcgata
    27241 atattgaccg aatcaaaagg aaaagggaca gatctcatgc agagctcgga aagtctgcat
    27301 ggagaagctg agatcattga taatccagct gacagtattg atcaaggtgt caaatatttg
    27361 gcggaaatga tagtagaagc aaagaaacaa ggatgcgatt tatggacagc tgtccaagca
    27421 tataattttg ggaaagatta tattgcctat gtaaaaaatc atggcggaga aaacacagta
    27481 gaattagcag aagcgtattc taaagatgtg ctgagcccac ttttaggaaa tgaggaaggg
    27541 aaacaatacc gatattggcg gatccagtcc ttgctttaca atggcggcta tctctatcat
    27601 aatggaggta acatgtttta tcctgatatt gtcaaaatga atgaaaaaat aatagagacc
    27661 tatgaaaaag tctttaagta aaatagcagg aaatgttcaa tcatgaataa aattaaatga
    27721 aagaaggagt gcgcaaatag tggaaaaaag gcaaaaaatg gaaaatcaag aagtggggta
    27781 cacaaccaaa gactggatgt tacggtttgt gaagggaatg tttatcggtt caggatttat
    27841 cttgccagga gtcagcggtg gtgcattagc agctgtcttt ggcatgtatg agcggctgat
    27901 ttctttttta gcccacctta caaaaaattt caaagaaaat gttttgtttt ttctgcctgt
    27961 tggtctaggc ggagtgacag gcatctttct actttctttt ttagtcagtt ttttattagg
    28021 tgcgtatgaa acaacgatat tatggttttt tgtcggatgt atcgctggaa ctgttcctgc
    28081 gttatggaaa gaagcaggga aaaaaggaag agatacttct gatgtgatca tcatggttgt
    28141 tacttttgtt ctggcttatt tgtttttatt atacggcgct caattatttg acgggcaagt
    28201 cgatcagaat tttggaacat ggctgattgc cggcggattg attggtttgg gtatgatcgt
    28261 cccaggattg agcccttcta attttttggt gtatatggga ttgtataaag cgatggctga
    28321 cggatttaaa gaagtacgac ttgaagtgat tgtgccgatc ggaattggtg ggattctttg
    28381 tgttcttgga ttgtcaaaaa tcatggatta cattttctca aaggcatata gcaagctatt
    28441 ccatttcatt ttaggtgtag tttttgcttc aacgattatg atcattccta ccgattacgc
    28501 gggattggga gttgctgggg tactagcttg tcctgttctt tttatagccg gtacagcact
    28561 aggttggtgg atgagccgtt tggaggaaca atataagtaa agatagattg ataataaaaa
    28621 cagcacacgg gtgattcaaa caaaaaggtt ggcatctttt ttgtaaaatc attcgtgtgt
    28681 ttttctataa attttataaa aacaagttgt ttttaatctt ggaacacgct agacttaaac
    28741 acgtgattat ttttgaaagt tggcggaaaa tggagaaaaa gaaaatgaat ggacttacac
    28801 agcaagaggt agttcagaaa atggcttctg gccagcaaaa tgactaccag gaagatgcat
    28861 caaaaagtac aaaacaaata ttcagcgata atatattgac gttattcaat tttctgaact
    28921 ttggtattgg gatttgtctg ttatttgtag gtgcgtattc caacatggcg tatttagcaa
    28981 ttattgtcgt caatatcgtt attggaattt atcaagaaat acatgcaaga aatcttgtac
    29041 ggaaattatc gattgtttcc caatcagaag tactagtgat ccgggacggt aaagaacaat
    29101 cgatttctac taaagagctg gtattaggag atttggtaaa aatcggagca ggagaacaga
    29161 taccagccga tttacaggtt atatctggac gggcagaagc aaatgaagct cttttgacag
    29221 gcgaatctga tttgattgtc aaagaaactg gagctgaatt gctttctgga agctttttga
    29281 caagcggttc tgtcatggct gaagttatcc gagtaggtgc agataattat gctgtccgct
    29341 tgactagcga ggcaaagact cacaaagcta ttcgttctga acttgtagat tcgattcgca
    29401 aagtatccaa attcactagt ttcgtgatta ttcctttggg gatcatacta tttttggaag
    29461 cactgttcat ccgcgatgct tctatcaaaa tttctgttat tgcatctgca gcagctttgt
    29521 taggcatgct tccaaagggt ttggtattgc taataagtat tgcattaacg acaggggtaa
    29581 ccaaactggc aaaaaaacga atccttgttc aagacatgta ttcaattgaa acgctggccc
    29641 atgtcgatac attatgcttg gataaaacag ggaccattac tgaagggaaa atgagtatcc
    29701 agcgggtaga agcactacat ccgctgtacg agaaagccat cccagaaatt atggggacgt
    29761 atctggcaga aagcagtgat aacaacatca ctatgcgggc tatgagagat tattttgagc
    29821 tttctaatcg gtatgatgtg acaaacaaag tcgcattttc ttctgatcgc aaatggggcg
    29881 cgatggaatt tccagaactg ggagtcgtgt acgtaggagc gcctgaacgt ttggtggaag
    29941 aagatcggct tccggacagt ctaaaaacag cccaagcgaa tggctatcgc gtacttatgt
    30001 tggggatcgc tccaaatcag caattgaacg aagaaaaacc agcatttgtt gagccggttg
    30061 ctctgtttga aatcgatgat ccaatccgaa aaaacgctca agaaacacta gcttatcttc
    30121 atgaggaagg tgtggatctg aaagtgattt ctggagacaa tccttttact gtctccaata
    30181 tcgctagacg tgcaggatta ccgaattatg atgcgtatgt cgatctttct caaattactg
    30241 aagaaatgga agtgcgagaa gctgctcatc gctatacggt ttttggacga gtgagtccgc
    30301 agcaaaagaa attgctggta aatgaattga aagaaagcgg cagaacggtt gcaatgacag
    30361 gcgacggtgt gaatgatgtt ttggcgttaa gagaagcaga ttgcagcatt gctatggcag
    30421 aaggtgatgg agcaacacgt cagattgcga atctggtatt gttggactct gattttacta
    30481 cattgccaga agtcctgttt gaaggacgcc gagtggtcaa caatgtgacg aaagtctcag
    30541 gcatcttttt tatcaaaacg atttattcat ttattttatc gattatatgt gccgttacag
    30601 caattggatt tccgtttatc cctatccaaa tcacgctgat tgatttagcg attgaagggt
    30661 atccttcctt ctttttatct tttgaacaag ataaaagaaa aatcacttac cgttatttgc
    30721 ctacagcact tattaatgct ttaccaaatg ccttgcttgt agtagtgaat atcattgcgg
    30781 tgtatctact tggaaaaaca ttcgcatggg gaaatgctga aactactact ttgatgtatt
    30841 acttgctgat tgggatcagt tgcatggcag ttgtaaaagc ttgttatccc ttcaacccat
    30901 tgcgcatctt cctagcagta acgacgatcg tagggatata tgtagctgca atgctgttcc
    30961 atggaatttt ggaagtaaac ttagggttgg gagagagcgt aggatacttc tttgtattca
    31021 tgctcgtaaa tattgttctt cgagtattgt actggaaggc tgatattcaa aatcagttac
    31081 tgaaaagagt agcaaattga tgcttaatga gatagaaatg tgttatactt gaaaaagcaa
    31141 tgaaatatga tacaatcaaa catgtataat tgtgtccttt aaaatcgcac tgatttcagt
    31201 tagtgcgatt ttttaccggt tattctcctg aaatatcttc tgttaaaccc aaaagatatc
    31261 aatagagcag ggggtgaaaa aatggctcaa cggattcctc aagaggttat cgaaacagta
    31321 cgcagtcaaa cgaatatcgt tgaggtgatt ggacaatatg tccagttgaa aaagtctggt
    31381 aaaaattact tggggctgtg tccttttcat gaagaacgta caccgtcttt ttctgttgca
    31441 gaagacaagc agatttttca ttgctttggt tgtggtaaag gcggaaacgt gtttactttt
    31501 cttcaagaac tagaaggtct aagttttcca gaagcagtgg taaaagtggc agacttcgaa
    31561 cagataccgt tagaagatca atggcgtcag cgtactatag ctgtccaaaa tcctgaatct
    31621 gctacgggga aactgattgc tgctcatgaa aaagcagcgg aaatctatca tcacatgcta
    31681 ttgcatacaa aagctggaca gccagcttta gaatatttgc aaagtcgtgg gttgtcttta
    31741 gaattaattg aagaattcaa cataggtttt gcacctaatg agcgttcttt tcttcagcaa
    31801 gtattcaaga atgaatcaat ttcaaatgaa ttgttagaac gctctggatt gtttgtcacg
    31861 catgatgatg gccgtatgtc tgatcgtttt tatcaaagag tgatgtttcc acttcgtaat
    31921 tctcaaggcc agacgatcgg tttttcagga cgctggcttg cacctgaaaa agggcaggaa
    31981 aaagatcagc ccaaatactt gaatagtcca gaaacagaac tgttcaataa aagacaagtt
    32041 ttatttaatc ttgataaagc aagaagcgat atccggaaaa acggagaaat tttgttgttc
    32101 gaagggttta tggatgtgat tgctgcatgg caggctggta tcaaaactgg attagcttct
    32161 atggggacaa gtctaacagc tcaacagatc caacaaatgg aaaagctgac gaaagaattg
    32221 gtcttttgct atgacggaga caacgcaggt tttgaagcaa ccaatcgagg gattgaattg
    32281 cttcggcaaa acagtcgatt agcactttct gtcgtagtcg ttccagaaaa gcttgatcct
    32341 gatgaatatc tccgtaagta tggagaagca tctttccaag aattagtggc tcatgggcgc
    32401 gaaacagtct ttacttttaa atcacgttat ttaaaacaag ggaaaaattt agataatgaa
    32461 aaagacaaga tcgaatacct cgaagaatta ctagttgaac ttagccgagt tgtttcacca
    32521 atcgaacaag atatgtatct atctcaactg tcaacagagt ttcagatcgg cagagagacg
    32581 atacaaaaac aactgcgcga tcttcgaagg acaaatcagc aaaatcgtca agaacctcag
    32641 gaaagtcaac acactcaggt tacgagacaa gaaactgcac ggaaaaagag acctttgacg
    32701 caagtagaaa aggcagagca gattctatta tatcgtgcat ttaaagaagt ttctgtccgt
    32761 aatattttaa aagaacgaga atttcagttt attcatgatg catatgcaga agtttatttt
    32821 ttattcgatg catatgtctc acaacattat gaattcaacc ttgcagagtt tttagacttt
    32881 ttgcaagatg aaaatttaag gcggttggtg gtggaaatcg aatatttacc ggtggcagaa
    32941 gaaagttcgc cacgagaaat ccaagacttg ctagatgtga tcagcaaatc tggcttagct
    33001 gatgaaataa cattaaaaaa acaaatgcag caagaagcca gacgaactgg aaacaaacag
    33061 cttgagcttg aattgacgat tgaaatcatc aatttaacga aacagttaaa acaagccaaa
    33121 tagctactca aggggggcct tcttttatgg caaatggaac agataaaaaa acgtatgaag
    33181 cagcagtagc agctttcatc aaagaaaaca aacccaaacg acaagttgta tatgatgatt
    33241 tatccaatca gcttgctaca ccattcactt tgaatgcaga tgaaatggac aaattgatcc
    33301 aaaagattga agacgcaggt atcagtgtgg tggatgaaaa tggtgatcca tcaattcaca
    33361 gcttgaagag tgctgagaaa aaagctgaac aagcaaaaac agaagatctt tctgcgccaa
    33421 ccggtgtaaa aatcaatgac ccggttcgta tgtatctgaa agaaattggc cgtgtctccc
    33481 ttttaactgc ggaagaagaa gtagcgttag cattgaaaat cgaagaaggc gaccaagaag
    33541 cgaaacagcg tctggcagaa gcaaaccttc gtttagtagt aagtatcgct aaacgttatg
    33601 taggacgagg catgcagttc cttgacttga tccaagaagg aaacatggga ctgatgaagg
    33661 cagttgaaaa atttgactac cgcaaaggat ttaagttttc aacctatgct acatggtgga
    33721 ttcgtcaagc catcactcga gcaatcgctg accaagcacg tacgatccgt attccagttc
    33781 atatggttga aacaattaat aaattgatac gtatccaacg tcaattgctg caagaccttg
    33841 gaagagaacc tacgccagaa gaaatcggtg cggaaatgga tcttccaaca gaaaaagtcc
    33901 gagaaatctt gaaaatcgcg caagaacctg tttcccttga aacgccaatc ggtgaagaag
    33961 atgactctca cttaggtgat ttcatagaag accaggaagc aacaagtcca gcagaacacg
    34021 cagcttatga gttactaaaa gaacaattgg aagatgtact agatacattg actgatcggg
    34081 aagagaatgt ccttcgctta cgttttggtt tagatgatgg ccggacgaga acattggaag
    34141 aagtagggaa agtatttggt gttactcggg aacgtattcg tcagatcgag gctaaagcat
    34201 taagaaaatt gcgccatcct tctcgttcaa aacaattaaa agatttctta gaataagcgt
    34261 ggactattga ccagaagaga aatcagtaaa actgtttggt ttattttaaa agaaaaaaag
    34321 cttgagacta cgcagtctca agcttttttt tctataggaa ggaaattcat tcattatttt
//
LOCUS       DASHRQ010000028        31764 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.028, whole genome shotgun sequence.
ACCESSION   DASHRQ010000028 DASHRQ010000000
VERSION     DASHRQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.028"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(273..740)
                     /locus_tag="U2M88_001422"
     CDS             complement(273..740)
                     /locus_tag="U2M88_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEM8373811.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(860..2398)
                     /locus_tag="U2M88_001423"
     CDS             complement(860..2398)
                     /locus_tag="U2M88_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEM8373812.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            2478..3164
                     /locus_tag="U2M88_001424"
     CDS             2478..3164
                     /locus_tag="U2M88_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEM8373813.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(3365..3892)
                     /gene="msrA"
                     /locus_tag="U2M88_001425"
     CDS             complement(3365..3892)
                     /gene="msrA"
                     /locus_tag="U2M88_001425"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEM8373814.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(3976..4653)
                     /gene="pgmB"
                     /locus_tag="U2M88_001426"
     CDS             complement(3976..4653)
                     /gene="pgmB"
                     /locus_tag="U2M88_001426"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEM8373815.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(4650..5780)
                     /locus_tag="U2M88_001427"
     CDS             complement(4650..5780)
                     /locus_tag="U2M88_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEM8373816.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(5780..7963)
                     /locus_tag="U2M88_001428"
     CDS             complement(5780..7963)
                     /locus_tag="U2M88_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEM8373817.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(7960..8985)
                     /locus_tag="U2M88_001429"
     CDS             complement(7960..8985)
                     /locus_tag="U2M88_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEM8373818.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(9030..9857)
                     /locus_tag="U2M88_001430"
     CDS             complement(9030..9857)
                     /locus_tag="U2M88_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEM8373819.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(9854..10921)
                     /locus_tag="U2M88_001431"
     CDS             complement(9854..10921)
                     /locus_tag="U2M88_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEM8373820.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(10947..11783)
                     /locus_tag="U2M88_001432"
     CDS             complement(10947..11783)
                     /locus_tag="U2M88_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM8373821.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(11780..12649)
                     /locus_tag="U2M88_001433"
     CDS             complement(11780..12649)
                     /locus_tag="U2M88_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8373822.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(12719..14002)
                     /locus_tag="U2M88_001434"
     CDS             complement(12719..14002)
                     /locus_tag="U2M88_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEM8373823.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(14016..15701)
                     /locus_tag="U2M88_001435"
     CDS             complement(14016..15701)
                     /locus_tag="U2M88_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEM8373824.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            15976..17001
                     /locus_tag="U2M88_001436"
     CDS             15976..17001
                     /locus_tag="U2M88_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8373825.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(17596..21270)
                     /gene="rpoC"
                     /locus_tag="U2M88_001437"
     CDS             complement(17596..21270)
                     /gene="rpoC"
                     /locus_tag="U2M88_001437"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEM8373826.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRGERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(21328..24945)
                     /gene="rpoB"
                     /locus_tag="U2M88_001438"
     CDS             complement(21328..24945)
                     /gene="rpoB"
                     /locus_tag="U2M88_001438"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEM8373827.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            25673..26641
                     /locus_tag="U2M88_001439"
     CDS             25673..26641
                     /locus_tag="U2M88_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM8373828.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            26659..27564
                     /locus_tag="U2M88_001440"
     CDS             26659..27564
                     /locus_tag="U2M88_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8373829.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            27561..28367
                     /locus_tag="U2M88_001441"
     CDS             27561..28367
                     /locus_tag="U2M88_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8373830.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            28503..29492
                     /locus_tag="U2M88_001442"
     CDS             28503..29492
                     /locus_tag="U2M88_001442"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEM8373831.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(29809..30333)
                     /locus_tag="U2M88_001443"
     CDS             complement(29809..30333)
                     /locus_tag="U2M88_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEM8373832.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            30620..>31764
                     /gene="scm"
                     /locus_tag="U2M88_001444"
     CDS             30620..>31764
                     /gene="scm"
                     /locus_tag="U2M88_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEM8373833.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDT"
BASE COUNT         8923 a         6866 c         5979 g         9996 t
ORIGIN      
        1 tttgaataaa cggtgttctc ctgccaactc ctcggtcaca agtcacaata tccataaaac
       61 ataagaagct gttttttaaa attgtttctt gtgactcgga gctgaacggc aggcttacaa
      121 cctcttggta aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa
      181 tatgaggaac tattttgacg tttgtttctc taatactcag gtcaaaacgc tgatttcaca
      241 acctcttttt cggtcaagtt caacaacagt ttttacgcgt cgatctctgg cgctttgccc
      301 aaatgagctt ggatcatcca gatacgtttt tctgtggctg ttttgaattc gataaagata
      361 tcttgtgttg gatcgtcgcc ttcttctcca gaaacttcga ttcctttttg gtaaagatct
      421 gctagataac gatagcctgc aacaagtgtt tccaaacgtt ctggaatagt acgatcccag
      481 cggccaattt catcagggat ttttgtgtta tctgcaaatt cacgcaatgt agaataaggt
      541 gaaccgtcta atgtgatcaa gcgttcggat acttcatcta actgagcgtt caaatcatcc
      601 atgaactcat ccatcattgg gtgcaatgta aggaaacctg gtcctctcat gtaccaatgt
      661 gtttggtgaa caacaacaga catttggctt agatctgcaa ctaattgatt taatacttct
      721 ttggtttgtt ggaatttcat ctttcttgcc tcctaacatt ttcttacact taaaataata
      781 gtagactttc aatgaaaagt acaacaaaat catttacctg agaacgattt tcagttcgct
      841 tttttatccg taaagccaat catttttcag aatcagctaa ttcttctcgc agttgagcaa
      901 attgctgata agtctgtgct aaatgctttt gtgtcgaaac aaataatgga tagattttct
      961 gatatttttt atgatcagtt tcttgtactc caacttgctc tttcaattct atcaattcct
     1021 gtgcttcact gatactttgg atctttccga tgcttctcca gcctaaaatc acagctccta
     1081 atgcggatgc ttctgtgaca gtagggaaaa caagcgggta acccagtaca tcagccatca
     1141 tctgacgaaa aatcggagat tgagcaaacc cgcctgtagc acggatctct gtgagctgat
     1201 tttcctggca aactcctttt aaaatccgat aaaggttgag acagatccct tctagaatcg
     1261 ccttcatcat ttctttttct gtatgttgcc gctgtaaacc gataaaactg ccaaacgcat
     1321 ctgcgtccca aagcggtgct cgctcaccaa gtaaataggg atgaaaaatc agaccattgg
     1381 cacccgctgg cacaaaagcg atagccgaaa gcagtgcatc atatggattt tctccttttt
     1441 ctttagcaga ttgaacgact tcctgtaaaa atgtatgccc tgcccaatca aagatgccag
     1501 caccattgct agtcgctcct cccactaccc aatgtttctg atccagaaca taacaaaacg
     1561 tctccatttc tggatggaac tgaggctgag tagtaataaa acgaagtgct ccgctcgttc
     1621 ctacggtcaa agcggcgatc gtatcaccaa aagcacctaa ccctagatta gaaagcggcc
     1681 cgtctgcccc tcccaaaaca aatgatatat catttggcaa acctagttct gctgctgttt
     1741 ttgatggtag acctgttact tgataggtgg aaggaaagac ttgcggcaat tgctctattc
     1801 gaatgttcaa gtagtcaagc acttcttctg cccattggaa ttcatggata tcaaaatatc
     1861 cagttcctga agctgttccg tagtcgctga tcagttcgcc aaacaatcta taaaagatat
     1921 agtctttgat accgacaaaa taagcagttt ttttgaataa ttcaggttgg ctgttttgca
     1981 gccacttgat tttgttcaat ggtgtcattg gatgaatcgg cgtcccagtc tgccgataaa
     2041 attctttgcc aagcacggta tatttcagtt tttctgcttc gtccgctgct cggttatctg
     2101 cccaagtgat cattcgagat aacggtgttt tctcttcatc caacaatatc aggccatgca
     2161 tcgcagagga gaaggagaca aattgaatga ttgtgtctga aggcaactgt ttaattactt
     2221 cttttataac ggtgcagaca ccttgaaaga tcaattcctg atcttgttct gccatccctt
     2281 gtgtttcttg gatcaaaggg tagcgaaaat aagcggatgc tgcttcttgg gcttcatctg
     2341 tgaaagcaac tgctttggtt tgtgtggttc cgatatctaa tccgattgaa acgttcattt
     2401 tttcacctca aattttatgt atgtacaaaa atttagttta agcttgcgta tcttggtttt
     2461 gaaaaggggg agatggcgtg ttactttatt ttatcattgg ttgctgtatc ggatcatttt
     2521 tatgtttaac cgcccagcgg attccgttag gtcactctat tatttatcct agatctcatt
     2581 gtgtcaaatg ctgtcgttcg ctttcttggt atgaattgat tccgattctc tcgatcatcg
     2641 tccaacgatt tcgctgccgc tattgcaggt gtcggttgcc cttctactat ttgttggcag
     2701 aagccttatg tggtggtctt tttgcctggt tctttacttt ttcttccgca agaaattttt
     2761 cgacgttcat atggatgctt tcggctttgc tattttcgtt gatggattta ttttacttca
     2821 tggtccattc tcacacgcta ttctgttctt gggcaatttt atggattttt tggttgcagg
     2881 ccggcgtctt ccaatggcaa agtgtgctgt taacgtttat cgtaggcggt gtgtgccttc
     2941 gctatggtca atcctatctc ggagctggcg atactctgtt gatgctttct tggagtggtg
     3001 gcttgtctct agaagagttg cttcagatct tgtttcttgc tagtctgttt ggaatatccg
     3061 ttttttctgt ttactttatc tgtcataaaa aaaagttgga aaagctgcct ttcattcctt
     3121 ttttgagcag tgcgttgctg attgtccttc atcttaggta ttgaaaaaag gttgtgacaa
     3181 acgacttgtc acaacctctg atatatagtg ttctcttacc aaatccttgt gacttggagc
     3241 taggcgacag gttcacaacc tctccactaa atggtgttca atcagctgac cctcagtatt
     3301 agctcaaaat gctggtttta caacctctga tatacggtgt tccataagta gctttttcgg
     3361 cttcttattt cttgacgccc caatgtcgac gaaggaaacc tgcacgtcca gaaccttctc
     3421 gataaagatt gtaatggagc ttgtttttct tgtaataatc ctgatggtaa tcttctgcag
     3481 gataaaattc gcttcttggt tcgatttttg tcacgattgg cttatcgaat cgcccgctgt
     3541 cttgcaatgc ttgttttgaa cgctctgcaa tatttcgttc ttcttcatca ttatagtaga
     3601 tcactggacg ataagaatca ccgcgatcag cgaactgtcc taatgcgtca gtcggatcgg
     3661 tttgctgcca atagatttcc accaactgat cataagaaat aacttctgga tcatattcga
     3721 tctctactgc ttctgtatgc cctgttgtgc ctgttgttac ttgttcataa gttggatttg
     3781 gtacatgccc gccagtataa ccggatgtca cagaaatgat ccctggctgc tcttcaaatg
     3841 gtttgaccat acaccaaaag catccgcctg cgaaaactgc tttttttgtc atataaaatc
     3901 cccctttttt ctcatcatac cttgatttcc aaaaggagga taacgttttg cttgtgacag
     3961 aaaaaacgga aactattata tacgtgatac ggcttctgat aaatgccaaa acgaacgtac
     4021 ttgatcaaaa acggttaaat caggaatcac atattcagca gaaggaagat ttatgttttc
     4081 acctattccc aatacatgca tgttaccagc caaagctgct tgacaaccag cttctgaatc
     4141 ttcaatgacc aagcatgcat ttggcggtac attcaattgc tgtgctcctt tgagaaaaac
     4201 ttctggatca ggttttgctt ttgatacttg cgtgccgtca ataaggacat caaaataact
     4261 tgtcaaattc gttttttcta aaatgaggcg tgcatttttg cttgctgatc ccaatccgat
     4321 cttgatgttt gtttttttca agtactcaag aacagctatt gcaccaggca aaacggatgt
     4381 ttcatccatt ttttgaatcg cttgaagata tagattgttt ttttgctcag caaaggctga
     4441 tttctctttt tctgagaatt tctgttccat ttttccaaat gacagcaatc gctctagtga
     4501 ctcggttctg gatattcctt tcagttgctc gttttctttc tcagtgaatg gaatggacag
     4561 ctggttagct aaaaccagcc acgcttcgta atgataacga gctgtgtcta caagaacgcc
     4621 atctaaatcg aatagtgctg ctttgatttt catcgattac ctccttgaaa atgaacgact
     4681 tgaagtaaag atgcgatgtt tttagctgtg cgatgaagat tatcagtggc ttgctgtaaa
     4741 gcttcttgta aaggcacagc tgcaggaagg atcggaaaaa cggccgaaat gccaacatct
     4801 gctgcttttt cgcttccttt accaacactt ccgcaaatag ctatcacagg aacgccctct
     4861 ggtactcttc tagcaatccc aactggcgct tttcctttta gactttgtcc atctagacgt
     4921 ccttcgccta cgattacgag gtctgcttgc tgtacccgtt tatcgaactc cagtaaatcc
     4981 aatacgtagt cgatgccaga gactattttc cctgaagcaa atgctaacag tcctgcaccc
     5041 attccaccac cagcactact gcccgatgcc tcgattactt gaggggcaaa tctcatataa
     5101 aaatcatgca tggcttggtc agccactgct aattcatttt ctttcaagcc tttttgtggt
     5161 ccgaaaacaa aagaagcgcc ttcttttccg cataaaagat tagtaacatc agtaatgaca
     5221 gtgattttgg ctttaatcag ctcaataggc acgttcgttt tatcaactgt agcaatttta
     5281 cctagatttt ctcccagtgc ttctactttt tgattatctt gatcgtaaaa agcataacct
     5341 agaccttctg ccatgccgat gccgccatcg ttggtactac ttccgccaac gccgatgatg
     5401 atgtgtcgaa caccttgttg caacaattgt cgtatcagtt ctcctactcc tcgcgtggat
     5461 aacctaagtg gctgttgttg gtttagtggg acatctttca atccgacgat ttctgccatt
     5521 tcaaaaatcg cagtttgacc gtctgaagcg tactggattt ctttgttttt gccaaatgca
     5581 ttagtaacga ctgctgtttg ccgcttcaac cttaaactgt ctgctaatga tgaaaccgtt
     5641 ccttctcctc catcgcctat aggtagcaga tcatattcag cctgaacaaa aacttcttga
     5701 aaacctgatt gaatggcttg tgctgcctct aaggctgtca gactttcttt gaaagaatct
     5761 ggcgcaatca gaatacgcat tattttcccc tccgttccaa gatcagttct ttttccagct
     5821 ggtattggtc tccattgaca aaaataggta atgtttcttt cgtctctttt tttattacta
     5881 tttgtttttt tgtcaaaaca aatagcaacc tgtgattttt ccaaataaaa ggaaaagaaa
     5941 ttttttccca tgctgaagga agtcttggag aaatagaaag agttccattc gttactgtaa
     6001 tacctgaaaa accaaatata actgctagcc atagtgctcc taatgaagca gcatgaattc
     6061 cgtcatctga tgaatgcgga tctggtccta aatcaatcag gcacgcttct tggaataaat
     6121 gatacgccca ttccatttct tggatacggc aagcctctat ggcgtgaatc gctttactca
     6181 acgatgagtc gtggattgtt ttgttttcat agtaatgcaa attcttagaa acgattggtt
     6241 gagaaaaata ctcaggtaat aagtataaca gcatgacaag atctgcttgc ttgctgactt
     6301 ggagttcatt tacttcgcta cgggcataat caagtaaaat agactggctg ccaggacgtt
     6361 ctttgtaagt ggtcaaatca ataactggct tactgagata cgtgtcatct tgcggaatca
     6421 gctgttcttt gttaggcact ggcaaatata acttatctaa aaaattttga cattctttca
     6481 aaaaaagcgg atcgccagct tgatatttaa cagctaaggc cacattataa tgcgccagat
     6541 agttcgtata tgcattattg tcgatatatt ctgtgtattc atctggacca atgactctgt
     6601 gtatctctaa atgatcatcg acttttacag ctctgctcag ccaaaaagcc gctgtttcat
     6661 gaatgagcgt ttttcccttt gtttctaaaa acgattgatc ctctgttgct tggacatact
     6721 cttgaacggc ataagcaagg tctgcactga tatgatgttc tgcggcagct gaagcaactt
     6781 cttgtcttgt tccggttttg atattcaatg cagcaaattt gggtgtctct tcctgaccag
     6841 tgtgtgcgct ttcccatggg aaaagtgcac cttgatagtg ataatctctt gctttttgcc
     6901 gtgcgccttc taatcgatca taacggtaca gcagcaaatt ctttgctact tcaggaaact
     6961 gatataaaaa gaaaggcagg ataaaaattt ccgtatccca gaaaacatgc cctttataac
     7021 cttcgccagt tagtcccttc gcccctacac taacacttgg atcattgcgg ggagtcataa
     7081 tctgtaaatg gtaagcagcg aagtccatcg ctaactgatc gaaagaagaa tcgccttgga
     7141 tcgttatccg atgctgatgc caaaaatctg tccaataaga catcgactgc tctaaaattt
     7201 ctgtataact aacgcttttc tgggcttggg caatactttt cttaaacggg tctgtttgct
     7261 gaagagaggt agagagcacg atgatcttct ccagcgtaaa ggacgtattt tttctgatct
     7321 tttctgaaaa ggaagcggtg atccggcgat tttttgcttg gaatacacct gctttattga
     7381 atttagctgc aatcccaata tcaattttac tttctgttgt ctggtaggat gcactgatca
     7441 aatcgtcacc aaatactcgc agttcttttt caaccaactg ctgtgtacca aaattggttt
     7501 gctgtccatc gatacccgtt ttcacatcta cttggcagtc ttcggtcaac ggcttgatga
     7561 ccactttcag ggctgcctga tggagattat tttgatcagc aacacgacaa gtaatgactt
     7621 ccactttttt tccttgagca gtttcaatga gcgcatagcg tttaagctct cccatttcta
     7681 aatctagtgc ccgctcgtat gcaagaactt tcatcgtctc aagagaaaaa tgttcaccgt
     7741 cgatttttat ttcccatctg atgacatctg ggagattcac taactcggct gattctgctc
     7801 ctaaaggtcg gttatataca ccagctgcaa acattcccct tacttgattc cgatagtctt
     7861 cctcctggct tcctctgata ccaaaaaatc catttccaat ggtcatctga ctcgcatact
     7921 tattgatata ctctaaatca aactgttttt cttctacttt cataaggtaa taacttctcc
     7981 tgtttctgcc gaacgatata aattttccac cagattctgg atgacatatc cttgccagcc
     8041 atctgcaatc atctgagtct ggtcaccgag cgcccgattc acaaaatgag ccatacttag
     8101 ttcatgtggg tcattaactg gcgtggtctt ttgtaaggaa accagctttc catctacatc
     8161 ttcgaatagt tcgcctggat acagattagc acctgcctga tcgccacaaa aagccacatt
     8221 cagtttgtct tctctgatat tcaaagcaaa cgaagtattc agttggatga tcttgccgtt
     8281 ttctaattcc agtgtgccaa aaattgcatc ttctactgtg aatttgcttg gatcccattt
     8341 cccgaatttc cctgaatttt ttttcgtacc aattttttgg aagctgtacg cccgaacgct
     8401 ttttacttta ggaaatccag taatatacat cgctgtatca agcatgtgaa tgccataatc
     8461 gatcaaaggc ccgcctcctt gaagttcttt gtcgataaac gttccccagc ctggaacacc
     8521 agaccgacga agcgcattcg cttctacgta ataaatatcc ccgagaacat caagatgatt
     8581 cttgatcatt gttgcttcgt cagaaaaacg atgctggaat ccataagcca aaatctgccc
     8641 gtttttatcg gctgtctctg ccatctttct tgcttgatca gctgtcatag ctggtggttt
     8701 ctcgcacatc actgaagcat tagcttcgag ggcagccaat acatgttcga agtgaaagcg
     8761 attgggtgta cagacactca cgatatcggg agaaatctca ttcagcattt gtgtaactga
     8821 tgtatacgca tgagaaaatc ctgtacgtcg gcaaaagtct tctgctcgct ggctgactgg
     8881 atcaaccaca gcggccaatt ctaagacatc tgatcttttt tgataataag cagcatgtac
     8941 tttttctgct acttgtccag cgcctatgat tgctactttc ttcatttatt tctctctcct
     9001 ttgcttattt acttattgtt attctttctc tattctttct ctaaatcatt taaatgacgc
     9061 gctgtttcca gacagttttt caagtattgc aatgattcct gataaatttt cacttcattt
     9121 tctcctcgca aacgacattc atataccata taaccttcat actcattttg aagtagcgtt
     9181 tgaaaatggc gagtaaaatc aattgcgcca gatcctggtt ggtagcgatg attatctgct
     9241 aaatggatat gacccacata ctctttttgc gtagtcaatg cttttgaaat atcatcttct
     9301 tcaatattca tatgataaaa atcagcgatg atctgcacat gtttcaattg attagagtca
     9361 atgtaatgac gcgcatccgc taacaaattg atcatatggt cttggtatcg gttcaaaggc
     9421 tccaaataaa taaccgtacc tgtttcttcg gctactttat ctaaataacg aagtgattcg
     9481 ctgactgctt tatagtctcc ttctttgctt cggggtgaaa ccatcggcgg taatcgataa
     9541 gtgaacattc cccaagctgc tgggataacg attcctttgc ctccaacctc gcttagtgca
     9601 cgcaaaatct cagcaatttg acgtacacca ttaagacgcc gttcttcgat aaagtcaccg
     9661 atccacccat cgtagccgtt acaagctgtc actactggga tccctgtttc tttgattgcc
     9721 tgtttgactg tgtcgatatt ttctattaac aacttaccgt cgatttcgta ttcttcaaat
     9781 ccacagtctt tgataaatct aaatttttct aaaatgtctc ttgggaaaaa tgcttgatct
     9841 tgcgtaccga atttcattgt tctcctcctc ggacttgaac gcccattttg atactttctt
     9901 ctgggtgctg gtctacataa cgcatgtaag cttctgcgct ttctgcaaat ggcacaactg
     9961 gatcaatgat ttcttcacaa tccaagtaac cgttcatcaa taattcccaa catgtgtcct
    10021 cgattcgttt acggttccat cttgggtaat ctgggtttgg ttcactagat gctcgagcaa
    10081 agacgatttt tgcattattg aaatgtgctt cgcgaccaag attaaaccca gctggaaaag
    10141 gtttagcaaa ggcaacataa gaaatgatcc cgccatagcc gatccctctt aaagaagctt
    10201 gtaatgcggc agcactgcca ctcgtttcaa taactgaatc tactccacgt ttatctgtca
    10261 attcctttag acgaaaaccg acatctgctt tcataggatc tagcgtgtaa tcggcaccgt
    10321 atttttctgc taattctcta cgctgcgcta tgggatcgat cccaacgatg gtagtagcac
    10381 ctgctttttt agctaattga atcgcaattt gcccgatagc gcctaaaccg aagattgcta
    10441 cagcatcacc tgctcgaaca tgtgcttccc ggataccacc taaagcgaat tgagcaggat
    10501 cgtaacatac tgcatttttc cattgatcca accgatcaag tttacgtagt ttataattat
    10561 caacaccgtt tgtaatcagc gtttcacgaa tgggtccata agtgcagact aactctcctt
    10621 tttgatactc tgtcaccaaa ctgcccgttt cttcgatcgt accgacaatc atatttccta
    10681 attgaaattc gccaaatacc acaccgcgtt ggcttccttc ttctcttggc aagaataggc
    10741 gccattcttc tgaaaattct tcatcgataa atggacttaa tcctctaaag tcgacaattt
    10801 ctgttcctgg ttttggagta gcgaaaatta ctcggatacg tacttcgttc ttttcgatct
    10861 cacggtcttc gtaatctttg aatccggcta ttcttggttc taatgcaact aattttttca
    10921 tcttttcttt cctctttctt ttctttttat ccttttacgc cgccttcgac taaatcacct
    10981 ttaataaatc gttcagataa cgcatacata atgatgactg gtaaagcggt caccaatgaa
    11041 gcggccatca ttcgtcccca aatgtagtct ggtgtactga ataatgtgtt caaaccgatt
    11101 ggtaaagtaa atttttcgga ggtcgacagg aaaatcgaag caaataaata atcattccac
    11161 gaaaccataa agcaataaac gaagacagaa atgattccag aaatagccaa tggtataatg
    11221 atccgaataa tgatctggat ccggctaaga ccatcgatca ttgctgcttc ttcaatatca
    11281 gtcggaatcg tgtcaaaata actttttagc atgaatatcg cagtaggaag tgtttgaacg
    11341 accatcgtga taatcagtgc tgtattggta tcatacaaac ctagaccgga aatgatttta
    11401 aataatggaa caacgatcaa aataccggaa aacatataaa cggtgtaaaa gctagcattg
    11461 attttcgagc gagctttgaa gcgtagtttt gacaaagcgt atgccccgct gatcccaatc
    11521 actactgcag ttacggaggc tatcaaggag atcactaaac tatttttgaa atacatgatg
    11581 aatggaaaaa tctcagcatt gaaaatatcc tgataatgtt caaaagtcca ttctttcgga
    11641 aaaatagttt gtgtcgttgc aattgcttct tgtgaatcct tgaatgatgt catcagcatg
    11701 atcaaaaatg gaaacaaatt gacagctaat agcaacaaaa tcgtcgcgta aaataagatt
    11761 ttcctgctct ttttattttt catttcaagt ccaccttctt tcgtgagagc aagacgaccg
    11821 ctgcaataat gatgaacaat agaatcgata tagctgctgc ttttccaaga tcattgaacg
    11881 caaaggctgt ctgatacaaa taaacgccta agatattgac tttattcgtc agtagataga
    11941 catctgtaaa catatagaac atccagatag atcgtaaaac aatcacagtt gctagtactg
    12001 gtttgattgc cggcagtgtc acgatcttga acttctgcca agcatttgca ccgtccattt
    12061 cagctgcttc ataaagcgtt ttgtcgactg tttgcaaaat agccaaaaat gaaataaacg
    12121 catacggata gtatctccag atagcaaaca aaacgaccat aaagaagcta gcaactggct
    12181 ggtcgaacca cagcggtgct tcgttgaaaa gctgcaacct atctactaac agatcattga
    12241 cgattccata actattattg aacatatatt tccaagcaaa aatcaaagaa atagatggcg
    12301 taacataact taataaaatc agtgaacgca ccgttttcct aaatctgaac ttgcggttaa
    12361 gcaaaatagc tacggctaat cctaaagctg tactgcctgc tacaactaag aatgtgtaag
    12421 caactgttaa acctaaagaa cgataaaatt cactatcact taaaatttca agataattgc
    12481 ttaatccgat aaaaacggcg tctgagttag gattcagcgg catatcgaaa aaactgatct
    12541 gaatattgga aagcattgga atcagcacta aaacagctaa taaaagcatg cttggtgcta
    12601 aaagtcccat ggctaatttt aagtcagaat gtttccttgg acgattcatt gaaacctccc
    12661 cctgataatc tgccaatagc tatctacttt tttcgaacac cattggcaga acttcttgtt
    12721 atttcgtatt tttttgtgcg tcagctaaag cttgatcgac atcttcgttt ccgacagtta
    12781 cttggttcac tgcttttccg atcacacctg agcttgtgat atctcccatt ttcgtgaaat
    12841 tcttttcccc gactagtcca aagacttgta cttcatcaaa agcactagca atctcagatg
    12901 gcagctcgcc aaaagacttg atgacttcat tttctttgta accatccagt tcgactactt
    12961 gtttattcac tggttgagca ccaccaggag acatcaagac ccacttttcc atgtttttcg
    13021 gttgagataa gaaagctgtg aatgcttcag cagcctcttt ttgtttctcg tctagaccag
    13081 aagaaatcgt caatccagat actgtgccat agacagcttg atctttttgt gttgggatag
    13141 caaaaccgat attttctgag ttccccgctt cataaacaga aggcaaaatg taagttgaat
    13201 agattgccat tggtgctgtt ccattcatga aagcatcttt gatttctgtc acatcattag
    13261 agcctggcat cgtgtattgg gataagtttt gataaaacga caatgcttct ctcatctttt
    13321 ccgtatcgat cgttacctcg ccttcatcat ccaaaacatt tgctccattt gacaaagcaa
    13381 attgagagaa cgcttgctca ctcattgttc cttcagcagt aggcatagca attccatatt
    13441 gtttattctc tttatctgtg aaatgttgag caatttttaa aatatcatcc caattttctg
    13501 gttctgaaaa accagcttct gataattttt ctttgtcata ccaaatacct tgaacccagc
    13561 cgctaattgg tgcagcaata tagctttttc catcttctga acgaacaagg tttttggctc
    13621 cgtcataata attatcttct ccagcttcct cgataacgtt ttggactgcc gtctgatcaa
    13681 tcagttcatc tttatccatc actttagcaa aatcctgact gacttccatt acttctggta
    13741 atttaccgga acgagctaac gtaacgattt ttgtattgaa cgctgattct tctaccggca
    13801 cttgcttgat tttgatgtct ggattttctt tttcaaaatc agcaaccagt tcattgatca
    13861 cacttaatcg ttcctgttcg acagaagagt gcatgaattg aatcgttgtc tgttgtgatt
    13921 cgttagagct ttgactttca ccacaagcag taagaagaaa tccagtagct agcagacagg
    13981 tagtaacaat aatttttttc atttttttcc acctcttatt ttattatttt cagccacata
    14041 tattcatacg gctggaatat caactttcgt tcttgactgc aatggataat ttgtccagaa
    14101 aacaggtcta ggtaatctcc ttttggtaaa agataacctg ctgattgatt ggaaaaatta
    14161 tggataacta agactttctc ttccttcgct tcgcgaataa aactgataaa agaatctgct
    14221 gagtccagta agtaaatcaa actattcgga tggaaggctt tttctttttt tcgaatagat
    14281 agcatctggg tcaattcacc atatactgct gttcttaatg gatctgtctg caactctttc
    14341 tcaatatctg ctaacaaaaa tttttgccga ttgatttttc gattctctcc tgttttttct
    14401 acgcctttgt aatcatttcg acttcccagc acactttgaa tataaattgc cggcacgcct
    14461 ggtaacgtta ccaaaaatga atgagcagct aaaaagcgtt tgcttctcca ttccgtcgta
    14521 tctgttggag aacttaacgc atctagatac gtagcgttta tctcatatgg actggtcgtt
    14581 ccatctgggt ttttcttgta gtttactcgg gcaccctctt tcttcagctg ttccactaat
    14641 tcaaggatcg ttttttccgg cagaatacct cgtaccggat tcaatccgat accgtcatgg
    14701 gaggctaaaa aattaaaaaa agttgttttt ttcgatgcga aatcgatggt ttttaaccat
    14761 tctttcaact tacttgtatt acccgaacga atcgcttgaa gggtcaaagg tggtagcgaa
    14821 aactgataga ccatctgtgc ttcattctcg ccattcccaa aataactgat attatctttg
    14881 tgtggcacat ttgtctcagt gatcaatacc gttcctggag ctgcttcatc agcgattgca
    14941 cggaataatt ggatgatttt atgcgtttct ggcaggtgta tacaagaagt tcctagttct
    15001 ttccataaaa atcccacagc gtccaagcga ataaatgaag cgcctttttc taagtaaaac
    15061 aaaagaatat cgatcatttt caataaaact tctggatttt taaaattcaa gtctacttga
    15121 tcttcgctaa aagtcgtcca tacgtgtttt tctttcccgt ttttgtcaat gaacgttgta
    15181 agtaacgggc tggtccttgg tctagtcacg ctgcttgtat ctgtatccag tggcaaatca
    15241 ataaaaaaat ctttgtacgt atcattcaag tttaaatatt cctgaaacca agtactttcg
    15301 gcagacatat gattgcagac aaagtcgaac attaaccgtg tttcgcttct catttcttct
    15361 atatcttccc atgtgcccaa acggtcgttg attgcctgat aatttacaac ggagaaacca
    15421 tcatcagatg tatatgggaa aaatggcaag aaatgtacaa ctctaaatgt atctgccaaa
    15481 tacttttgat aaaatttctt aaatgtacgc aaagttggtt gctgctcttc aaaaaattga
    15541 tccccataag taatcaagaa aatatcttct tcgtcccaat gcagtttgcc tgcctggcct
    15601 ttcttctgct gataaacact gactttttgt tcaatcatag aggcggtttt ctcagacaaa
    15661 tctgagtcgc gataaatcgc ttgtaaaagt tttgtataca tcggattccc ctttcttttt
    15721 tgggagcgtt cccaacgatt tgatcatatt atggaaaacg cttactgtca atagcatttt
    15781 tttaaataat tttgatatac acaaaaatac aaataagaaa gcttatgtat caataattaa
    15841 tagcttctta aaaaaatatg gtctctcatg atcatgcaag ctattcctaa attttcaaaa
    15901 tcatagacaa aaaagaatgt ctattgtact atggaattat tctaaaacgc tcccaaaaag
    15961 gaggtgcata taaaattgaa acctactatt tatgacattg cgaaaaaagc taatgtctca
    16021 aaatcaactg tctccagagt attaaataac cagccaaata tctctgataa agcaaaagca
    16081 agtgtacttc aagcaatcaa agaattgaat tatgaaccga gcaaaattgc aagaggactg
    16141 tcaggaaatg gttttgacgc gattctagca ttatctcatc gtacttctca tacgacctat
    16201 ggcaatcctt ttttttcaga tgttattcaa tccatttctg ccgtttctga acaaaataac
    16261 ttcgacttga ttctacaaac ggcaaaaaca ccagaagaag agctagaaaa atgcgcgaaa
    16321 aagatccaag aaaaaatgat ccgaggaatt attattttaa gctctacgat ggatgaaaca
    16381 cttctttttg aattggatca atacaatatt cctattgtgg taatcggaaa agtcggaaac
    16441 gactacacga acatctactc agtggacacg gataatttcc aggatagtta cgatttggtg
    16501 gaaacactga tcaagaatgg acaccgtcat atcggctgtc tccatgctcc gacagaagtt
    16561 tatgtatcca atgatcgtct caatggttac cgtcaatgct tatttgatca ccacctagat
    16621 atccgtaatg aatggattat agacggcggc tttcgcttag aagatagcat ggcagcaatg
    16681 aatgatttaa tgaagctacg agaaagacca actgcgattt tttctaccga tgcattaaaa
    16741 tcattgagta tttacaaagt ggcagaacaa tctggaaagc agatacctga tgatctttcg
    16801 atcgcaggat tcagtgatcc cttattgtcc tccctatttt cgccacagtt gactcggata
    16861 gacattccga caaatcagtt aggaatcaaa gccacagaac ttttattcca actaatccac
    16921 caccaatacc ctgaagaaaa aagaatcatt ctttcaaccg agattactct cacagattcg
    16981 atccaacaac aagaaatgta gaacagaatt ataaaagaac agaaacactg aggataagtc
    17041 agttactctg ttcttttttc aatcactcat ccctcgttac cccctcccgg tattttagcg
    17101 aatttttctt attttccatc tgttttagat agctgtggat aactataatg tttgtgttaa
    17161 actatttttt ccacagggaa gaaaaaacta tttttaacca cgttgtgtat aagtttttat
    17221 agttcgtttt tttaaacaca tttaccctta tttcaatcca tgttatttgg ggatacttat
    17281 ctctaaaaca taaatagaat aacagacatg tctactggtt ttgttttatt cgaggaatta
    17341 ttcaataata gctagcaaaa tattaaaaat aaccgctcta tcttcccatc aatttatttt
    17401 gcattcaaaa aatgatctat ctttactttt gttgattgac ttctcttgga agatgatcgt
    17461 tccgatacct taattggata cgaaccgatg ttgttaagat cctttcccac ttttcttcta
    17521 gttagacaaa ttattttaga caaaccaaaa aagaggttgt gacaataatt tgtcacaacc
    17581 tcttttttaa aatatctatt ttgtttgatt gttcattgcg tcttctgcag ccatttgcgc
    17641 ttcaatatct gaaatactgt agacattttc gcttgctaca ccgacttctt ttggttccat
    17701 gtcacgataa cgttgcatac cagtaccagc tgggatgatc ttacctataa taacattttc
    17761 tttcaaacca agaagaggat ctttcttacc gcggatcgca gcatccgtca agacacgagt
    17821 tgtttcttgg aatgatgcag cagataagaa actatttgtt tctaatgatg cttttgtgat
    17881 acctagtaag acaggtcgag aagttgcagg aacgcctcct gagactagcg tattgtagtt
    17941 ttgatcttta aagtctgcga tgtctaataa tgtacctggt aagatatctg tatcacctgg
    18001 atccattaca cgaactttac gcagcatttg acgaaccatt acttcgatat gcttgtcgcc
    18061 gatttctacc ccttgcatac ggtaaacacg ttgtacttca cgaagcaagt agttttcaac
    18121 agataataca tcgcgaactt gcaacaattg ttttggatcg attgatcctt ctgtcaatgg
    18181 tgcaccacgg tggatcatat cgccttctgc tactttcata cgtgctgtat aaggaacagt
    18241 atacgtacgt gtatctgttt ttcctttgat tgttacttct ttggtacgtg ttgcaggatc
    18301 ttctgagata tcgatgacat cccctgttac ttctgtgatc actgcttgcc ctttcggatt
    18361 acgtgcttca aagatttctt ggacacgagg aagaccttga gtgatatcgt ctccggcaac
    18421 cccacctgta tggaatgtac gcatcgtcaa ctgagtacca ggttcaccga ttgattgagc
    18481 ggcgatcgta ccaactgctt ctccgacttc aacgtcagag ccagttgcaa ggttgcgtcc
    18541 gtaacaatgt ttgcagacac catgttttgt attacatgtg aagacagaac ggattgtcac
    18601 tgtttcgaca cctgcatcaa cgatttgttt tgcgatgtct tcggaaatca attggtttcc
    18661 tgcaatgatc aattcatttg tttcaggatg acgtacttct ttacgggtgt aacgtcccac
    18721 taaacgatct tctaatgatt cgatgatttc atttccctcg cggatggctt ggatttcaag
    18781 acctcggtca gtaccgcaat cttcttcacg gatgattaca tcttgggcca cgtcaaccag
    18841 acgacgagtc aagtaacctg aatcggcagt cttcaaggct gtatcggtca tccctttacg
    18901 agccccgtga gttgagataa acatttccaa gacggatagt ccttcacgga agttcgaaat
    18961 gattggcaat tccatgattc gaccattagg agcggccatc aaaccacgca ttccggcaag
    19021 ctgtgtaaag ttggaaatgt taccacgagc tccagaatca gacatcatga agattggatt
    19081 tttcgcttcc aaaccttcca tcagtttttg ttggatgctg tcttttgcag cattccaaac
    19141 agcaatgaca cgttcataac gttcgtcatc tgtgatcaga ccacgacgga attgttttgt
    19201 aatagtttca acttgtttat gggcattttc aatgatttct tgtttttcat tcagtacact
    19261 gatgtcagcg atccctactg tgataccggc atgtgtcgaa tgtttgtacc ctaagtcttt
    19321 catacggtca agcatcttag aagtttctgt tactttgaat cgtttgaaga cttctgcgat
    19381 gatgtttcct aagtttttct tcttgaacgg taatactaat tcttgctctt tgatatgtgc
    19441 aggaatatca gtaccagctt caacaaagta tttgtccggt gtttggacgg tcaagttgaa
    19501 gtctgttggt tcattcagat aagggaattc aggaggcatg atctcgttga agatgatctt
    19561 accaactgtt gtgatcatcg tacgttcttt ttgccattca gtaaatggtt tttcacctaa
    19621 tgagttaggg ttcacaccga tacgtgaatg taaatgaaca tatccgttgc gccatgcgat
    19681 aacggcttcg ttttgatctc tgaagaccat tccttcgcct tcgcgtcctt cttcttccat
    19741 tgtcaagtag tagttaccta gaaccatatc ttgagaagga gtaacaactg gtttaccatc
    19801 tttagggttc aagatgtttt gagcagctaa catcaacata cgtgcttctg cttgtgcttc
    19861 ttcattcaat ggaacgtgga cagccatttg gtctccatcg aaatcggcat tataagcttc
    19921 acatacaagt ggatgcaaac ggattgcgcg tccttgtact aaaactggtt cgaatgcttg
    19981 gataccaagt cggtgaagcg taggtgcccg gttcagcaat actgggtgtt ccttgattac
    20041 gtcttctaaa acatcccata cttcgtcttc ttgacgttcg attttacgtt tcgcattttt
    20101 gatgtttgaa gctagttcgc gttgaacaag ctcacgcatc acgaatggtt tgaataattc
    20161 aatggccatt tcttttggaa gtccacattg gtacatcttc aagaaaggtc caacaacgat
    20221 aacggaacga cctgagtagt cgacacgttt acctagcaag ttttgacgga aacgaccttg
    20281 tttccctttc aacatatgag aaagagattt caatgggcgg ttacctggtc cagtaactgg
    20341 acgtccgcga cgaccgttat cgatcaatgc atctactgct tcttgcagca tacgtttttc
    20401 attttgaacg atgattccag gtgcattcaa gtctaacaaa cgtttcaaac ggttattacg
    20461 gttgattaca cgacggtaaa gatcattcaa gtcacttgtt gcaaaacgtc caccttctaa
    20521 ttgaaccatt gggcgtaaat ctggtggaat aacagggata acgtccataa ccatccagct
    20581 tggttgattt cctgaagcgc ggaatgcttc taaaatatcc aaacggcgga ttgcacgtgt
    20641 acgtttttgt ccttgtgcag atttcaattc ttctttcaat tcggctgctt ctgcatctag
    20701 atcaacatta tcaagtaatt gtttaatcgc ttctgcaccc atagcagctt ggaattcttg
    20761 tccatattgt tcacgttttt cgcgatattc gcgttctgtc aacaattgtt ttttctctaa
    20821 attcgtattt cctggttcga tgactacata tgaagcaaaa taaatgattt cttccaaagc
    20881 gcgtgggctc atgtctaata caagacccat acgtgaagga atccctttaa agtaccagat
    20941 atgtgaaaca ggagctgcta attcaatatg tcccatgcgt tcaccgcgaa ctttcgaacg
    21001 agtaacttcc actccgcatc gatcacaaac gattccttta taacggatac gtttgtattt
    21061 tccgcaggca cattcccagt cttttgtagg accaaaaatc cgctcacaga acaacccttc
    21121 gcgttcaggt tttaaggtac gatagttaat cgtctcaggt ttttttactt caccgtaaga
    21181 ccagcttctg attttttcgg gagaagccaa acctatttgc atactttcga atttatttac
    21241 atcgatcaaa aggggttccc tccatttcat ttaatggcaa accgcaagac ggctgatctt
    21301 cgaccgcctt gcagctcata gaccggatta gtctaaatta tcttctgcgg tttcaaagtt
    21361 ttggatgaca tcgtcttttt catcttccac ttgttcagca gctttctttt ctaattcttt
    21421 ggcagtttgc tgttcggcaa attttgtcaa agcatcaaca gtgatcaaat cgtcgtcttc
    21481 atcgtccatg tcgcgaagtt cgatctctgt ttcttcgata tccaagacac gcatgtccag
    21541 acctagtgat tgcaattctt tcaccaatac gcggaatgat tccggtacac ctggttttgg
    21601 aattggttca cccttgacga ttgcttcgta tgttttcaca cgtccgacta cgtcatctga
    21661 tttgtatgtc aagatttctt gtaaagtata agccgcaccg taagcttcca gtgcccatac
    21721 ttccatttca ccaaaacgtt gtccaccaaa ttgagcttta cctcccaatg gttgttgtgt
    21781 aaccaatgag taaggtccga ttgaacgtgc atgcaatttg tcatcgacca tgtgagcaag
    21841 tttgatcatg tacatcacac cgacagacac acgtccatca aatggttcac ctgtacgtcc
    21901 gtcgtacaga acagttttcg catcgctggc cataccggct tcacgaactg tttcccatac
    21961 gtcttcatca cttgcaccat caaatactgg cgttgctacg tggataccta actgacgtgc
    22021 agccataccc aagtgtaatt ccaatacttg cccgatgttc atacgtgaag gcacccctaa
    22081 aggattcagc atgatatcga ttggtgttcc atcaggtagg aatggcatat cttcttccgg
    22141 cataatacgg gatacaaccc ctttatttcc gtgacgaccg gccattttat ccccttcatg
    22201 gattttacgt ttttgaacga tataaacacg aactaacatg tttacgcctg gtgacaattc
    22261 atctccagct tcacgagtaa agattttcac atcgtgaacg ataccgccgc cgccatgtgg
    22321 tacacgtaat gatgtatcgc ggacttcacg agctttttcg ccaaagatcg catgcaatag
    22381 acgttcttct gcagataatt ctgttacccc ttttggcgtg actttcccta caagcaaatc
    22441 gccatcttgg acttctgcac caatacggat gatacccatt tcgtctaaat cttttaatgc
    22501 gtcttccccg acattaggga tttcgcgtgt gatttcttca ggtcctaatt ttgtatcgcg
    22561 ggcttctgat tcatattctt caatatggat agaagtgtat acgtcatctt ttaccaaacg
    22621 acggctcatg atgatcgcat cttcatagtt gtacccttcc caagtcatga aaccaactaa
    22681 aacgttttgg cctagagcca tttcgccttg ttccatagat ggtccgtctg ccagagtatc
    22741 ccctttttcg actttctcac ccaaatgaac gattggacgt tggttgtagc ttgttccgga
    22801 gtttgaacga cggaattttg ttacatcgta tttatctaat gcaccgttgt cgcggcgtac
    22861 gcggatttca gaagcatcaa cgtattcaac gacaccgtca tgtttacata acaaagcagc
    22921 tcctgagtca tgcgctgatt tgtattccat accagtacca acccatggtg cttgaggatt
    22981 gatcaacggc actgcttgac gctgcatgtt ggcacccatc aaggcacggt tggagtcatc
    23041 gttttccaag aaaggaatac atgctgtcgc aacggcaact acttgtttag gtgatacgtc
    23101 catgtagtct actttatcaa tagatacttc taagttttca ctttgagcac gagccataac
    23161 aacgtcttga gcaaatgttc catcttcatt caatgggctg tttgcttggg ctacgatgta
    23221 atggtcttcg atatcagctg tcaagtagtc gatttggtca gtaacacgac ctgtttcacg
    23281 gtctacacga cgataaggtg tttcgataaa tccgaattta tttactttcg cataactgga
    23341 caaactattg atcaacccga tgtttgggcc ttcaggagtt tcgatcggac acatacggcc
    23401 atagtgggag tagtgaacgt cacgaacttc ataaccggcg cggtcacgag tcaatccgcc
    23461 aggtcctaag gctgaaagac gacgtttgtg tgtcaattcg ccaagagggt ttgtttgatc
    23521 catgaactgt gacaactgag aagaaccaaa gaattctttg atacttgcca caactggacg
    23581 gatattgatc aattgttgag gtgtcaatgt ttcagtatct tggatcgaca ttctttcacg
    23641 aaccacacgt tccatacgtg ctagcccgat acggaattgg ttttgtaaaa gttcaccgac
    23701 tgaacggata cgacgatttc ctaagtggtc gatatcatcg acattgccga taccttccat
    23761 caagttcaag aaatagctca ttgaagcaat gatgtcggct ggacgaaccg tttttacagc
    23821 tgcttctgga tagccgtttc cgatcacgtt tacttcacgt tcagggtctc ttggtgaaaa
    23881 tactttgatg acttggacag tcattggatc tgtgaccaca ccgtcttctg atggatagta
    23941 agtcactgaa tttaggccat tatcaatgaa aggagctagt gtttccatga cttggtgagt
    24001 cagaactgtt cctttttcaa cgatgatctc gcctgtttct ggatcaacta atgtttcagc
    24061 taatgtcaga ttcaatagac gtgttttcaa atctaatttt ttgtttactt tgtaacgacc
    24121 aacgtttgct aagtcgtaac gttttggatc gaagaaacga gcattcagca agttacgtga
    24181 gctgtctgct gtttttggtt cgcctggacg caaacgttcg taaacgtctt tcaaaccttc
    24241 ttctgttctt gagtcgcttg cgtttttatg caagtctttt tcgactgtat tgcgtaatgt
    24301 ttcgctgtcg ccgaagattt caaagatcgt atcatcggaa ccaaatccta acgcacgaac
    24361 taatacggtt aaaggaatct tacgtgtacg gtcgatacgt acataagaga tgtcttttgc
    24421 atcagtttcc atttctagcc aagctccacg gttagggata acagtagaac cgaatccttc
    24481 ttttccgttt ttgtcgactt ttccatggaa atagacacct ggtgaacgga ctaactgaga
    24541 aacgattact cgctctgccc cgttgatgat gaatgttcct tgttctgtca ttaatgggaa
    24601 atcaccgaag aatacttctt gtgctttgat ttcgccagtc tcgcggtttg tcaaacgcaa
    24661 tgttacatgt aatggtgcag agtagtttgc atcatgcgca cgagcttctg ctactgtata
    24721 ctttggttct ttcaattcat aatctacaaa ttccaaagat agattgccat tgaagtcatc
    24781 gattggcaaa atgtcttcaa acatttctct aagtccctca tctaagaacc attggtaaga
    24841 gtctgtttga atttcgatca aatttggtaa ttccaacact tcactgattc ttgcgaaact
    24901 tctacgttcg cgatgttttc cgtattttac tacgtgtcca gccaagctct tcacccctct
    24961 aaaatgtcat tcaaaaagac tacccgatca attgttacaa taatatttca aatattattt
    25021 ttaggtaaca acagacattt tttgcggctt ttaggcaaaa aaaaaccaaa aatagaacat
    25081 tctcttgaaa ccttacttcg tgagcttcta cttttgtttc cttatattaa agccgaaacg
    25141 gacaatattt cgtttctgcc ttttcttcat cagatagttt ttgcaacttc atatactaca
    25201 cttattttct ataaaagtca acacttcctg gcgtttcttc tgctttttcg caacattttc
    25261 ttaatgtttt caattttatg ttttcaatta tctgacaatt cttaattgac agaccttttt
    25321 atctcggtta tactttagaa aattcatttt gtggaggaac ttctatgcga accaacatca
    25381 accaaatcgc accaacacaa ctgaataact tttactttag ctactttaga taggtttgta
    25441 ttcatcatga tggatacaaa cgttaaacga cagtttgtat ctatgatggc taaagaactt
    25501 tgtattgcag caaaaagcca cgtagacaaa atgctaagtg gctgagcagt gaaaatacaa
    25561 agtaaaagta tcaagcattc tactttttca atttattttc cgacttttca atccatttgg
    25621 acattttgtc tgaatggatt ttttgtttcc caaaattaag gaggaaaaag aaatgaaaaa
    25681 aaatataatc atcggattag gcgctgccct gctactagct ttaggaggtt gcgcacaaaa
    25741 agacaattcc gcagaggcat cagatggaca aaaaacaatt ggcgtattgc aacttgtaca
    25801 acataactcg ttagatgctg cctataaagg attcaaagaa ggattagcag aagaagggta
    25861 taaagaaggc gataatctaa caatcgatta tcaaaatgcc caaaacaatc aagacaacct
    25921 gaaaagtatg agtgagaaac tcgtcaaaga agatcctgat ttgctacttg gaatcgccac
    25981 tccagcagca caatctttat taaatgaaac aaccactatc ccgatgactg tcacagcggt
    26041 cacagacttg gaacaagccc atttagtcac atccaatgaa aaacctggcg gcaatgtgac
    26101 tggcacgaca gatatggtgc caatcgaaaa acaaatcggc ttactgttaa atatcgtgcc
    26161 agacgccaaa acaatcggca tcatgtataa caacggtgaa gcaaactcta agatccaagc
    26221 tgatttggca gaaaaagaat tgaaaaaagc gggcgtcaaa gtcaaagtac tgacagcaaa
    26281 ttccacaaat gatgtccaac aagtgacgaa aagtctagct aaagatgtag atggaatcta
    26341 tattcctact gataacacgt tcgcaagtgc ggcaagtgtt gtcggtgaag tcgcaaaaga
    26401 aacaaaaaca cctgtcgttg ctggcgcagt tgatcaagtc aaagacggcg gtctagcaac
    26461 agtaggaatc gactacgaaa gcttgggcaa acaaacaggg atcatggcag caaaaatctt
    26521 agacggcaag aatccaagtg aacttcctgt cgaaacagcg aatgatttgg aactgtatgt
    26581 caataaagac atggccaaag cattaggcat caatccagac agcatcaaag aagctgaata
    26641 aaacacagga ggattctcat gacaaatttg ctctctcttt tcttatcatc tgcatcgcaa
    26701 ggggtcttat gggccctact tgcgattggt gtatacgtca ctttccgtat cttggacatt
    26761 gccgacttga ctgctgaagg aagctttcct ttaggtgcag ggatcgcggc agtaagtatt
    26821 acaaatggct atcatccatt gacagcttgt cttctaggct ttttaggggg tgcggcagca
    26881 gggcttgttt ccggactgct tcacacaaaa ttgaaaattc ccgcccttct tgcagggatc
    26941 atcaccatga ctggtttata ttctgtcaca tcccgtgtga tgggtgcgcc gaacatctct
    27001 ttattgggtc agccaacgat ctttcattgg gtcgagtcat tcggcataac aaaagacaac
    27061 gctgtactga tcgtcggtct gctttttgct ttgatcgttg tatgtttact tgttttgttc
    27121 ttacgtacag aaacaggact tgctattcgt gcaactggcg ataatctggc aatgagcgaa
    27181 gcaaacggga tcaacacaga taatatgaag atcatcggtt atatgatttc taatggatgc
    27241 attgctcttt ctggctcctt acttgcgcaa aacaacggct ttgctgattt aaattctggt
    27301 attggaacga tcgtaattgg acttgcttct attatcatcg cagaagtttt gttacgtaac
    27361 cagccactat ttctccgtct cttgactgtc gttttaggtg cggtcattta tcgttttatt
    27421 ttagcgttag tcttcgaact gaatgttgaa ccaagtgatt cgaaacttgc ttctgctttc
    27481 gtgctagtga tctgtctttc tttcccgcag atccagcaaa aattccattt acctcaaaaa
    27541 aagaaaggcg gtagtgtcaa atgaatgacg tcttaacatt ggaaaatctc catcatacct
    27601 ttgaaaaagg aacgatcaac gaaaatcatg tcctgaaagg cattaatctt tccttagatc
    27661 ccggcgactt cgtgacgatc atcggtggaa atggggctgg taaatctact ttgctgaaca
    27721 gcatcgccgg tacattttct gtcgatcagg gtcaaatttt attgaatgga aaaaatatca
    27781 cgaaaaaatc tgttgtcgaa cgttcgaaat ccatcagtcg cgtattccaa gatcctaaac
    27841 tagggacagc cgttcgttta acggtagaag aaaatcttgc tcttgctatg aaacgaggaa
    27901 aaaaacgcgg cttcttccgt ggtgtcaaac cacaagaccg aacatttttc aaagaccagc
    27961 tggttcggct aaatttgggg ttggaaaatc gtttatcctc agaaatcggt ttgctgtctg
    28021 gaggacaacg tcaagctatt acgttattga tggccacatt acagcgtcca gaactgatct
    28081 tattagatga acacactgca gcgcttgacc ctgctacttc caaaacggtg atggaattga
    28141 cagaaaagct gatcactgag cagaatctca ctgcttttat ggtcactcat gacatggaag
    28201 acgcgatccg ctatgggagt cgactgatca tgctgcatca aggaaggatc gtcgtagata
    28261 tccagcaaga ggaaaaacag cagctcacag tgccccaatt attagcgttg ttcaaacaaa
    28321 acagcggcac ggcattaaaa gacgatcaag tacttttatc ttcataatca gaaagaggat
    28381 gggacaaaaa atgaacgccc accctcttct tttttctctt tccttctttc gtttttgctt
    28441 tatgtatcaa ccgttgtttc ttcgaaaact attttgttct ctccttcttc ctgatagaat
    28501 ggatgggaaa ggagcttgat gccatgacaa cgaaagacaa ggtacttcaa ttattgaaaa
    28561 agacaccaga ttttctttcc ggagaaaagt tggcacagca actggaagtt tcccggacaa
    28621 gtatttggaa agccatcaaa gaattggaaa aggcaggcta tcgttttgag cacgggccta
    28681 cagggtatcg ttacctgccc tctgacgtac tagatgcaaa agaaatccag caagagctac
    28741 ggtcgtttat tcctgatttg aaagtacaaa cgatgttgac ttctgaatct acgatgaaag
    28801 atgccaaact ggcagcagct caaggagatt ctgtttcgaa gttatttgtt gctgatacac
    28861 aagaacaagc tcgtggacgt tttggacggc cttttttcgc tgaacctgga cgaggaatct
    28921 acatgagtct cttgctccat ccagacaaac ggtttgatga attgcctcaa tatacgatca
    28981 tcgcagccag tgcctgtgtg aaagccattg aatctgtaac cggtcgttct gcttcgatca
    29041 aatgggtcaa tgatatctac atggatggaa aaaaagtctg tgggatttta tctgaagcag
    29101 tcagtgatat ggaaagcggc aggatcagtc atatcatcat tgggatcggg cttaattttt
    29161 ctgttcctca aaatcatttt ccagctgatc tccaagaaaa agccatctct gtctttcccg
    29221 acggacaagc tagtgtcaca aggaatcagc taatcagcaa aatctggcaa gaattgttca
    29281 cttatctttc tcacccagaa aaagcgttgg acttctataa agaaaaatca ttcgtattgg
    29341 gaaaaaccgt gtcgttcaat caaaaaggaa cgacttatgt cggtcttgca aaagaaatcg
    29401 gtgatcatgg cgaactgatc gtccaaacaa atgaacagaa gatgagtttg tcttctggcg
    29461 aaatcagtct cacatctatc cgtacgcaat aattatcaga tttcacaaaa ggacacctga
    29521 tctcatacag tgtccttttt tctgaattct cttcaaaaga ctctccttat tctatcagct
    29581 gacagtttct ctatcctcta gaaaaaggta ttctaaaaat atctataaag catggtacac
    29641 tttctttaaa tgaatcaatg caaaaaacaa gagagtcatc agtttacacg atcaaaagag
    29701 atagcaggcc gcttcctttt tcaatcttat agatagacca tgggtaacga taaaaacaaa
    29761 aaagaacttg cgacttattt tgccgtaagt tcttttttag ctagtgcctt aatctcttaa
    29821 tcccaacaaa cgtctcgcat agatcacctg accagagtgc atagcagcat catcaataat
    29881 agaaacgaga cgcattcctc gtgtgactgg tggtgtccag ttctcgtcaa ttacttcatc
    29941 tagctcggaa ggttccagcc ttcttaagta agaaatagca aaatcagcag ctcctcgcaa
    30001 gtatcctagc agatcttcta tttgattagc ttggatctgc tttgcttgtt ccagactatg
    30061 cttccaatct tgttcgttat ctgctatcgt catcggaaac cggctttccc agtcttgact
    30121 atgccaaatt ggagaagttc ctgctaaatc agcaatctgg aaatctaatt ctcgagcggt
    30181 atgccaagcc agccaagtca ttgatttgat ctgcggggct ctatcttcca ctggaaaacg
    30241 attggcctcc tcaatagaga cgccttcaaa caaacgttca aaccgatcgt atgcccgatc
    30301 cacactctcg catagcatat ctaaataatc catttatttt cctccttttt atttatccta
    30361 cttacttttt cctaattcta ggatacctac ttatatgtta ctacacaccc ttatttttta
    30421 cactttaaca gtcaccatca tcaatagttt ggagattgaa agaaaaagat agtgtcgaac
    30481 tatcaattga acaagaagca acaatatgaa cgtttcatta atattcttta aataaacaat
    30541 gatttttcaa acgaacagtt taagactagg agaaaaagag tataatagga aaatgtttga
    30601 aaggaaagga aggggaaaaa tgaaaaaaag ggcaagttta ctagtcctag ttgctttgtt
    30661 gttcaatatc ttttcgtatg gtgtggccgt ttcagcggaa acatacattc ctaaagaccc
    30721 tgggaatatt tataatgatc taactggtaa ctatggcttg ttaggaattg ctagtcaatt
    30781 ccacgtattt gctaaaaata agacaactgt gaatgcacat acagacggaa atatagcaac
    30841 acatgaactt gatgcaaaaa ataattttgg tacagagctt catgaaggtt tattgaacca
    30901 agaaataaat tatatacaaa aaactgattc tttcgctaat tcttcaggca tacctacagg
    30961 tagcacaaga atgaataaat ttgtcgttgg cagtaacgtg gatgtagcac ttcaagatgg
    31021 tcatcctaca gtcaatggat ccaggcttga tcacttaact ccagatgatt tttataaaga
    31081 tcgtgctggg catgaatata ttgatttcga gcaggaattt gctaaattga atgtagcagc
    31141 aaatgatctt gcaacgatca ctccagctaa aacatatact gctgcagatt ttccagacat
    31201 gaacaatcgg acaatcgatt taactgattt atcagatacc gggtttttat tggtaaatat
    31261 cgatgcagaa gtgttaacga tgaatacgcc acttcaaatc attaatccaa atgatcaagt
    31321 agttgtattt aatgtcatca acagttcttc tgcattgaac gtacagtcac caatcaaata
    31381 caatgatcgt tcaaatcatg aaacagaaga tttttcggat gcgaatatct cttggaattt
    31441 tggaaatgag atgactgatt tgacaatcag tgctcctttt caaggaacaa tccttgcgcc
    31501 taatgctact atccgcatta ctcaaaacca agacggaacg attataggaa caaatgtgat
    31561 tttagatgca gctaccaatc gttgggatcc taacgaaata tttatcagcg acaatggtac
    31621 agatacgaca gacacaagcg acagtacaga tacatctacc acaagtgata gctcagattc
    31681 atcaacttca agcgacagta cagatactac ctctaccaca agtgatagca cggatacatc
    31741 agcttcaagc gacagtacag atac
//
LOCUS       DASHRQ010000029        30792 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.029, whole genome shotgun sequence.
ACCESSION   DASHRQ010000029 DASHRQ010000000
VERSION     DASHRQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.029"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(138..302)
                     /locus_tag="U2M88_001445"
     CDS             complement(138..302)
                     /locus_tag="U2M88_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373834.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            597..1925
                     /locus_tag="U2M88_001446"
     CDS             597..1925
                     /locus_tag="U2M88_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEM8373835.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2127..2960
                     /locus_tag="U2M88_001447"
     CDS             2127..2960
                     /locus_tag="U2M88_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEM8373836.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2976..3713
                     /locus_tag="U2M88_001448"
     CDS             2976..3713
                     /locus_tag="U2M88_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEM8373837.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3868..4836)
                     /locus_tag="U2M88_001449"
     CDS             complement(3868..4836)
                     /locus_tag="U2M88_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEM8373838.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5068..5901
                     /locus_tag="U2M88_001450"
     CDS             5068..5901
                     /locus_tag="U2M88_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEM8373839.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5935..6774
                     /locus_tag="U2M88_001451"
     CDS             5935..6774
                     /locus_tag="U2M88_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM8373840.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6800..7360
                     /locus_tag="U2M88_001452"
     CDS             6800..7360
                     /locus_tag="U2M88_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEM8373841.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7559..9280
                     /locus_tag="U2M88_001453"
     CDS             7559..9280
                     /locus_tag="U2M88_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEM8373842.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9445..10587
                     /locus_tag="U2M88_001454"
     CDS             9445..10587
                     /locus_tag="U2M88_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEM8373843.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10603..11814
                     /gene="thiI"
                     /locus_tag="U2M88_001455"
     CDS             10603..11814
                     /gene="thiI"
                     /locus_tag="U2M88_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEM8373844.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12387..13034
                     /locus_tag="U2M88_001456"
     CDS             12387..13034
                     /locus_tag="U2M88_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEM8373845.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13163..13379
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13427..16072
                     /locus_tag="U2M88_001457"
     CDS             13427..16072
                     /locus_tag="U2M88_001457"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEM8373846.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16382..17695
                     /locus_tag="U2M88_001458"
     CDS             16382..17695
                     /locus_tag="U2M88_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEM8373847.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17685..18338
                     /locus_tag="U2M88_001459"
     CDS             17685..18338
                     /locus_tag="U2M88_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEM8373848.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18393..19085
                     /gene="radC"
                     /locus_tag="U2M88_001460"
     CDS             18393..19085
                     /gene="radC"
                     /locus_tag="U2M88_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEM8373849.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19235..19435
                     /locus_tag="U2M88_001461"
     CDS             19235..19435
                     /locus_tag="U2M88_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEM8373850.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19689..21368)
                     /locus_tag="U2M88_001462"
     CDS             complement(19689..21368)
                     /locus_tag="U2M88_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEM8373851.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21370..21582)
                     /locus_tag="U2M88_001463"
     CDS             complement(21370..21582)
                     /locus_tag="U2M88_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEM8373852.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            21975..23705
                     /gene="efrA"
                     /locus_tag="U2M88_001464"
     CDS             21975..23705
                     /gene="efrA"
                     /locus_tag="U2M88_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEM8373853.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23702..25462
                     /gene="efrB"
                     /locus_tag="U2M88_001465"
     CDS             23702..25462
                     /gene="efrB"
                     /locus_tag="U2M88_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEM8373854.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            25557..25754
                     /locus_tag="U2M88_001466"
     CDS             25557..25754
                     /locus_tag="U2M88_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373855.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            25907..26392
                     /locus_tag="U2M88_001467"
     CDS             25907..26392
                     /locus_tag="U2M88_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEM8373856.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(26496..27089)
                     /gene="clpP"
                     /locus_tag="U2M88_001468"
     CDS             complement(26496..27089)
                     /gene="clpP"
                     /locus_tag="U2M88_001468"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEM8373857.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            27269..27796
                     /locus_tag="U2M88_001469"
     CDS             27269..27796
                     /locus_tag="U2M88_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373858.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            27887..28624
                     /locus_tag="U2M88_001470"
     CDS             27887..28624
                     /locus_tag="U2M88_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEM8373859.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            28628..29545
                     /gene="miaA"
                     /locus_tag="U2M88_001471"
     CDS             28628..29545
                     /gene="miaA"
                     /locus_tag="U2M88_001471"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEM8373860.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            29547..30776
                     /gene="hflX"
                     /locus_tag="U2M88_001472"
     CDS             29547..30776
                     /gene="hflX"
                     /locus_tag="U2M88_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEM8373861.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10172 a         5266 c         6568 g         8786 t
ORIGIN      
        1 taaataaagc ttatgcaaag aaaaagaaat ccaatcactt gggtttcttt ttcttttatt
       61 attatgatga atcaagcttt ctgtttttgt cacgtatgtt gtaatcttta cgaaagagta
      121 gagttggcta ctcataatca accatacttc ccatcatcgc taaatacgaa ataaatcagc
      181 attcctacac caatcaaagc acaagctgca ccaagaaaag ggatgacatt cccaaagtta
      241 tctgtattca taagtatgcc accacccaca aacaaaaagc cgccagcaat caggtttttc
      301 attttattca tctcctttct tacgttatag tatttccttc tttcaatgag tattttgaaa
      361 gctatcttat tgtatgatgt tcttctaggc ctcttcttta ttatacaggc cattttcgtt
      421 atctcataga aatattcctt tttatctgaa aactattgta taccgtatga catttttctt
      481 catgaagttg taagtatatt ctcattcaaa tgaaaatttc atcatagaag agattgtatt
      541 tctaaattct taagagtaaa atacgaagta taacttggat ttggaaggat gattggatga
      601 aagctattac gcctgatatg acaacaaaat ttaacaaaca gtttcaagca aatatcaaac
      661 agcaggcatt gcagcgggca gttgtgaaga acggaattac tgcttctgct gaaaatgttt
      721 cagcacatgt ggagaataca ccggtatttt ctatagattt ggctacgggg aaagtggcta
      781 atcaaatgca atcggggcgt tgctggatgt ttgctgcact gaacacattc cgtcataaga
      841 tgctgaatca attccattta aaagagtttg aattatcaca aaattacact tttttctggg
      901 ataaatatga aaaggcaaat tatttctatg aaaacatcat tgcaacagga aaagaggcaa
      961 tcgatagccg gaaagtagct ttcttactcg ctactcctca acaagatggc ggccaatggg
     1021 atatgatcgt ttcattattc caaaaatatg gcgttgtgcc taaatcggtg atgccggaaa
     1081 gtgcgaatag ttcaaattct cgagatttga ataactactt gaacaaatta ctgcgtaaag
     1141 atgcagtagt gttacggaaa atggttgctc aaggagaaac attagacgct attgaagaaa
     1201 agaaagaaga aatgctagaa agcatttata acttcttagc tatctcatta ggaacacctc
     1261 caaaagaatt cgattttgag taccgcgatg aagaaaagaa ctatcatctt gaccgaggac
     1321 tgacaccaca aattttttat gataaataca tcggtgtaaa attagatgat tatgttagcg
     1381 tgatcaatgc accaacaaaa gacaaaccat tcaatcgttc ttatacagta gagatgctag
     1441 gaaatgtcgt tggcgggaaa gaagtcaaat atctgaatgt tgatatggaa actttcaaaa
     1501 aattagcgat tgctcagctt gaagaaggag aatctgtttg gtttggttgt gatgtaggtc
     1561 aatcttctac tagaactagt ggcatcatgg ccttagatgc ttattcaatg gatgatctat
     1621 ttgatacgga ttttacaatg actaaagctg aacgtttaga ttttggagaa agtctgatga
     1681 cccatgcgat ggtattgacc ggtgttgact tagtagacgg gcaatcaaca aaatggaaag
     1741 tcgaaaatag ctggggcgaa aaagttggaa agaatggctt ctttgttatg agtgatgcct
     1801 ggatggacga atatacgtac caaatcgttg taagaaaaga tttattgacg aaagagcaat
     1861 taaatgcgtt caatgaagaa ccaattgttc tatctccgtg ggatccaatg ggtgctttag
     1921 catagacgag tatttcaatg aagagtatgt agtaaagata aaaagagctg gcttaagcca
     1981 gctcttttta tctttaaagt ccattccctg ttttttatgg tccctgtgct ataattctaa
     2041 ataaaggata aggattggaa ataatccttt tatcattaaa aatatgatga cgaagtagaa
     2101 taaaattggg agtagggggg aaagtcatgg atatccaaac tgttttatct gatctgaaat
     2161 taaatggtaa agtgattcca gttgttggtg gcgatgttaa tcagacctat cgtataaaaa
     2221 ccgaacatcg tgcttatttt ttaaaaatcc atccgaatgt aaaaaaagga ttttttgaag
     2281 cagaagtaga cggattaaaa gaactgtcag cattcgtacg tgtaccggat acgtatatgc
     2341 taggtgaaac aagtgaaggc gcttatttgt taatggaatg gatcgagccg ggaaaagggg
     2401 atcaacgaga tcttgctgca gctttagcta atctccatca gcaaacagcg ccacaatttg
     2461 gttttcgtaa agataactat ttgggtacac ttgttcagaa aaattctttt gaagaagatt
     2521 ggtggacttt tttctttaaa gatcggcttg aatcccagat ttctttggca gaagaaacga
     2581 atcgatggaa tgtacagcgt caagaaaagt atctgcgatt taaagaacgt gtattgaaat
     2641 cggttgaacc aaaaaagatc actccacgcc tcttgcacgg agacctgtgg agtggtaatg
     2701 tgttctttga ccagcaaggg catccagttt ttgtcgaccc agctgtttct tatgggaatc
     2761 gggaacaaga tattgcaatg agccagttat tcggtggttt ccgaccggaa tttttagatg
     2821 cttatcaaac tatatttcca ttagaaaagg gatggaaaga ccgactgccg atttaccagt
     2881 tatattatct tttggcacat ttgaatatgt ttggagaaag ttacggttcc caagtagatc
     2941 aattactaga aaatttttaa gaggagggcg tgtttttggc aaaaaaatat accaaaaagc
     3001 atgaagtttc atattatgag tgtgacatca accaaacgat gacatttcct tcgatgttag
     3061 gagtagttat caaaacgtct gaagatcaaa gcgaccagtt gggaagaggc agtgaattcg
     3121 tcaatagctt tggacttact tgggtcatca cgaattattc catggagatt aatcgtctcc
     3181 ctagagtcgg tgaagtaatc tcagtaacga ctcaagccat ggagtacaat aagtttttct
     3241 gttatcggaa cttctggatc catgatgaag atgggaaaga attagtcaaa atagaatctg
     3301 ttttcgtatt gatggatttt gtgaatcgaa aaatgagcag tgttaatgaa gagatcattg
     3361 ctccttttga gagtgagaag atcaagaaaa tcaagcgtca ggaaaaaatt gaaaagattg
     3421 attcgggctt catgttgcca tatcgtgttc gtttttatga tatcgatagt aatcagcatg
     3481 ttaataatgc gatgtatttt aattggatca ttgacgtatt aggatatgac tttttgacta
     3541 cgcatgttcc agaaaaagta attattcgat ttgataaaga agtagagtac gggaatgaga
     3601 tcgaaagcca ttttgaacaa atccctatcg aaaatggtgt caaaacaaaa catgaaatcc
     3661 gaatgcaaga acagctgtat tgtgaagcaa atattatatg gaaaaataaa taaaaaagag
     3721 gttgtgacag aagggggcaa cttcaagaaa taagaagaaa tttacgaaaa ttgtttctca
     3781 aatttttgta aattttcgac ttagttttcg aagaagctat ttttgtcaca gcctctcttt
     3841 ttaagtttag ctattagata atgatgttta gttttggata aacgaaatca attcttcacc
     3901 cgacaaacca tagtttacag caatcaatag ttttaaacgt gctttttgac tattaatact
     3961 gttacagaaa ataatcccca ttttttctag ttcttttccc ccgccttcat aatcataaac
     4021 aggctctgcg attccattga aacaacgaga aacaagtact acagggattt tttggacaag
     4081 aagcttttcc aaagcagcta aggcttgtgg agggagattc cccgcaccta aagcttcgat
     4141 taccaaaccg tctactttcg tatgggcgat ggcttccaat aagtcccctt gcatcccagc
     4201 atatgcttta acgatcggga tagtcccatc tacagcggaa atgtccagtc gctttgtttc
     4261 taaaagttcc tgcaagaata aaattcgatt cttagttact aagccgatgg gacccaatgt
     4321 aggtgttcgg aaagtagcaa catttgtggt atgggtcttt gtcacgtagc gtgcagaatg
     4381 gatctcatcg ttcatgacca ccaaaacccc cttatctagc gcttcttcac aacttgcgac
     4441 acgaatcgca ctttcaaaat tataaaggcc gtcacttcca agttcattac ttgaacgcat
     4501 tgccccagtc aaaacaattg gtaatttgtt gccaatcgtt gtatcaagaa aaaaagcagt
     4561 ttcttctaat gtatctgtac cgtgtgtgat caccactccg gatatacctg atgcataagc
     4621 ggacaagata cgttctttta ataccaacat gtgagaaggc atgatgtgtg gtgagggaag
     4681 attgaagata tcttcaacta ataattctac atgcttgggg attgtgattt ctgcatctaa
     4741 taatgggttt gccacatcag gactaacacc gcccgtgagc atgttttctt tcatggcgat
     4801 cgttccgccg gtgtgtagta caagtatttt tttcatttta cagacttcct tacgaataat
     4861 tttgcatagc cgttaaatct tattgtatac tatataaaag agttttgcta tcagtgaaga
     4921 ataacgaata gcctaactta tcataaaaaa gctgacctta ttaaaatgga aaagactcaa
     4981 ccttgacctc ctaatacaat ggagtatctg gttgagtttc ttttacaaga aagcagccaa
     5041 ggaaatcaat cacgaaggtg gccggaaatg atcagaatcg gattaacatc gtttaaagaa
     5101 catgacaaat taactggtaa aaagaataac actctttatg aatatgcagg gtttctaccc
     5161 ttagtggaat tagatactgc ctattatgga attccccgaa agtcctcagt tgagaattgg
     5221 ataagtgaag tacctgaatc atttcgcttt gtagtcaaag tctatcaagg aatcagcggt
     5281 caaggagact ggaagcctta ttatcagacc gaagaagaga tgattgcgca ttttttagat
     5341 gttatgtcgc caatggtaga tgctggaaag atgttttgct ttttagtcca attcccctca
     5401 tcattcaaat gtacgaaaga aaatgtccag tacttgcgca aacttagaaa gtggtttgag
     5461 ggttatcctg tttctataga attgcgggat tattcttggt acagcgatga atttcttgaa
     5521 cgtacaagaa actttatgtc tagagccgct ttttctttag ccatcatcga tgagccacag
     5581 ttgctgaata caacgattcc ttttgatcca tttgtcacca atccgaactt tgctcttttt
     5641 cgattccatg gtaggaatgc gcaaggatgg caagcaaatg ataaagagtg gcgtaaaaaa
     5701 agaacgttat atcgttattc agaaaaagaa ctgcaagaac tagcacatga agtcaaagta
     5761 gttgattcta agacaaaaga ggtaggtgtg atttttaata ataattctgg tggtgatgcg
     5821 gctgaaaatg cattagcact gcaaaaaata ttaaatatca agccagatgg actcaatcca
     5881 acccaaatgg atctgtttta agggcttatt ttattcaaga aaggggcgaa gtctatgatc
     5941 aaagctattt tttttgatat tgacggaaca ttggtcacca atcgttcaaa agcgttggaa
     6001 tcaacaaaac aagcaattga atatgctcgg aaaaacggca ttttatgcgg tgtagcaacg
     6061 gggcgcagcc cagtaaagat caaagaaatc attgatgaat tggaattaga tatgtatgtc
     6121 gtgtataatg gtcaactggt ttttactgca gatcgcacga ttatcgacca cccttttgaa
     6181 caaaaagtat tggaacatat tgttgaattt gcagatgaga accatcgaca aatcgttttt
     6241 ggtgcgagaa atcggttaga tggatcaacg acgatgctat taggacaatc gatttttatc
     6301 aagcgactag tcagcttttt accgcgtaaa ttcccggtac gtctgatgaa aaaaatcctc
     6361 caggttttta gccctcaccg acaaaaggat cgttatgaaa cgttagagat attaaaagaa
     6421 ccaatttatc aatgtatatt gctcagtccg gagtcagaac agaaaaagtt agaacatcgc
     6481 tttcccgagt gtacgtttca gcgttcaaat acgtatactg tagatatcat cccaaaaggt
     6541 ggttcaaaat tactaggcat ccaagctttc gcaaatgctg tcgaaattga aatggaagaa
     6601 attatggcat tcggcgatca ttacaatgat atcgagatgc tgaaaggtgt aggaattgga
     6661 gtggccatgg ggaatgctca gatcgaagta aaacatgctg cagactttgt gactcaatca
     6721 aatgaagaag atgggatata cgaaggactg aaacacttcg gtctgattta ttagtcaatg
     6781 aaagaaggaa aaaatcagta tggaagcaga gacacctttt caaaaagtag aaacattcca
     6841 caaaactttt gatccaagaa gaccagagat acccacggct tttacagaaa atgaagctca
     6901 tacacgtgct gcgttcaaaa tagaagaact tgttgaattt gttgcagcta cttcacctaa
     6961 taaagaacaa ctgcatgaag ctgttatgct gctgcacgaa gcattagata aagcagaaga
     7021 aaaaatactt ggtaaaacac agtggggaag tgatccgctg gttgaacaag tagatgcact
     7081 gacagatatc ctttacttta cttatggttc tttttcatta ctaggaatag tgcctgatcg
     7141 aatattcgaa atcgtccatc aggcaaatat ggggaaatta tttccagatg gaaaacctca
     7201 ttatgaccct gtaacaaata aagtgttgaa acctgaaaac tggcagatgg aatatgctcc
     7261 agaacctaaa atcgcggcag aaatccaacg tcagattcaa gaagcgattg ctaagaagaa
     7321 atccgctgat ataggcatgt gcacaaatga aaaagagtga tcagagtttc actatttttg
     7381 ttatctttat acgatggaaa aacaaaagat tttcgtttaa gttagtgaat gcatgaaaat
     7441 agtatgctat aatctagaat agatgtcttt tgcacgagag acgtattatt tggatttatg
     7501 ccaagcagaa cgccatctgt tgcataaaag atgcatactg aaaacggagg aaccataaat
     7561 gggaaataat gttatcatcg gaatcgtcat tgcaatcatt atagtcgcca ttgtcgtctt
     7621 tttggttagt tacttgatga agaaaaaaaa ccaagatcga ttggatgcct tagaaaaacg
     7681 caaggaagaa ttatttgact tgccagtgat tgaagaagtc gatgatgtta aaaaaatgca
     7741 tctggttggg caaagccaaa atacctttcg tgaatggagt caaaaatgga atgacatatc
     7801 tacttcctcg ttcgctgaac tagaaagtca aattttcgaa gtggaaaacc tcaatgaaag
     7861 ttttcggttc ttaaaaggaa aaaaagcagt tgttgaagca gaagctacaa tggaccaaat
     7921 ggagaaacaa gtgacggaaa tccgtgaagg tctaaaggaa ttgcgggaaa cggaagaacg
     7981 caactcacta gctgttcagc aggcacttga cgtctatgaa gaactgaaag aaaaagtcag
     8041 agaagacgag catgaatacg gaccagctct ttttgaattg caaaaacaga tcaaagtgat
     8101 cgaaaaagaa tttacgcagt ttgtgacttt aaacacttct ggagaccctg ttgaagcccg
     8161 cgaggttttg gataaagcag aaaaacatac gtatgaagtg gaagctctga tgaaaaaaat
     8221 tccaccgctg tatgaggagc tccataccac tttccctgaa caacttgaag aaatcagtga
     8281 tacttacgag aaattgatgg aagaacaata tgtattccca gaagaagatc ttgctgaaga
     8341 tatcgcaaaa gtaagtcgaa gaattgaaaa ttcattagcc aatcttgaaa aaacagaagt
     8401 tgaaacggtc gaatttgaaa accgtgaaac agctgatttg attgattcgt tatatgatat
     8461 cttagaacgg gaaatggaag ctcagagata tgtaaaaact aaccaatcaa cgattgcaga
     8521 gtatatcaaa catacaacaa agaataaccg tcaattattg attgagctgg atcacacatc
     8581 tcaaagctat acattgaacc acaatgaaat tggccgtgta cgaggtttcc aaaccgaagt
     8641 ggaagaaatg gaacgtcaaa atgaacagat gattccacag atccgtcaac acgaaattcc
     8701 ttattcagaa gtgcgcacgt tctataaaac agtcttcaaa gtcttagaag atatcgaaaa
     8761 gcaacaagta gagatcgacg actcattaca tgaattgaga aaaggcgaaa aagaagcgca
     8821 agaaaagatc gatacattcg aattcaaatt gcgcagtttg aaacgttttg tagaaaaaca
     8881 acgtttgcct ggcttgccga atgattacct agaattcttt tttgttgcta ctgaccgtat
     8941 tgaagaacta agtgttgtct tgaataaaat tcgagtaaac atggaagagg tcaatcgttt
     9001 agttgctcta tgtgaagaag atctagaatt attagataag aagacacatg atttagtaga
     9061 tgcagcagca ttaacggagc aaatgctcca atatgcaaat cgttatcgtc atacacacga
     9121 agaagtgaga gaagcaattg ataaaagcta ctatttattc aataaagaat atcactatca
     9181 agaagcatta gacgaaatcg gtacagcatt agagcgagta gaacctggtg ctttcaaacg
     9241 catcgaagat ttttatttta atcatccaga cttagtataa agcaaaaaag ttagtaaacc
     9301 agcaagagca agacggatta ttgtcttgtt cttgtctttt tttgtatatt tgaaaattga
     9361 aacaagtgaa aatttcacct gctcttgaaa gcaccttgga caaccgttat aatagaaaag
     9421 aatttcaaaa aagaggggtt tcttatgata tattttgata acagtgcaac aaccccaatc
     9481 tttccgcaaa gcttagatgc atatgtaaaa accagtcagc ggattattgg gaatccatca
     9541 agcttgcatg atttaggcaa tcaagctaac cggttgttgc agcaagcaag aaagcagatc
     9601 gctgatttga tccatgtaga accagaagaa atctatttta cttcaggcgg aacagaagga
     9661 gataactggg tattaaaagg aacgatgatc gaaaaaagag aatatgggaa tcatatgatc
     9721 atttcaggag tcgaacatcc ggccgtcagc gagacagcag aacaattaaa agaattagga
     9781 atcgaagtaa gtatcgcccc agtggataaa cgcggatttg tacgtgtcga cgaattgaaa
     9841 gaattaataa gaaaagagac agtattagtc tctgttatgg ctgtgaataa tgaagtagga
     9901 tcgattcagc ccatccagga aatcagtgat cttttggatg attttcctaa aattcatttc
     9961 catgtagatg ctgttcaggc gattggaaaa gtaccattct cgcaatggtt gacagaaaga
    10021 gttgattttg caacattttc agctcataaa ttccatggcc cacggggaac tggtttcatt
    10081 tattggaaaa agggtcgtcg tttagcacca ttactgacag gtggagggca agaaagaaat
    10141 cagcgaagtg ggacagaaaa tgttccaggt attgtggcta tggttaaagc tttacgttta
    10201 tcattagaga taaaggaaca aaaacctgag cagacacaac gcttaaaagt agctctaatc
    10261 aatgcgttga agacgtatga gaaagtgacg attttttcag aaggaactga ctatgcaccg
    10321 catattttat gttttgctct aaaaggaatt cgcggcgaag ttctcgtcca tgccttagaa
    10381 gaaaaacaaa tatttgtttc tactactagt gcctgctcct cacgtaaaca tatggcaagc
    10441 agtacattgc acgcaatgca tgtaccaaat gatctggcta catctgctgt tcgaatcagc
    10501 ttagatgaaa acaattcgct tgcagaagtc gagcaattta tgattatttt caagcagctt
    10561 tataagaaat tttctgcaat caattaaaag aggagtgttc ttgtgaaata cagtgaaatt
    10621 atggtccgtt atggggaact ttcgacaaaa ggaaagaatc gaaaaagctt tatcatgcaa
    10681 ctagcacaaa atgtcagaca agctttgaaa gactttccag aaatcaaaat ccatgctgat
    10741 cgggaccgga tgcatctttt attgaatggc gcagacgggc agctagttat tccaaaacta
    10801 gcaaaaatat ttgggatcca aaatttctct ccaagtattc gtgtagaaaa agatgtcgaa
    10861 gttttgaaac aagctgttca agacatcatg aaagaaattt atactgggaa agaaacattt
    10921 aaaattatcg ctaaaagaag cgatcaccaa tttgaattga caagtaatga attgaatcaa
    10981 acattaggta atgctgtttt tgatattttc ccgcatatcc aagttcaaat gcgtcaacca
    11041 gatattccat tacgtgtaga gattcggcgt gatggcgctt atctatcgta tgaaacgatc
    11101 aagggagctg gaggattgcc cgtaggaaca agcggaaaag gaatgcttat gctttctggg
    11161 gggattgatt ctcctgtagc agggtacttt gccatgaagc ggggagtgga aattgaagct
    11221 gttcattttg ctagtccgcc gtatacaagt gaacaagcac tgcaaaaagc aaaagatcta
    11281 acagccaaac tagcacctta tgtgggaacg atccatttta tcgaagtgcc atttaccgag
    11341 attcaagaag aaatcaaaaa gtcagtgcca caaggatatt ggatgacaat tactcgtcga
    11401 atgatgttgc gtttgaccga tgaaattcgc cgtattcgtc atggtttagt cattttgaat
    11461 ggtgaatcat taggtcaagt ggcttcacaa actttgcaaa gtatggtggc tatcaatgaa
    11521 gtaacgaata cgccaatcat tcggcctgta gcgacaatgg ataaacttga aatcattgaa
    11581 gtagcagaac agatcgatac atttaatctt tctatccagc cttttgaaga ttgctgtacg
    11641 atttttgctc ctccacagcc aaaaacacgc cctcaactgg aaaaggtcca tcaatatgaa
    11701 gagcgtttag caatagatga aatgatccaa cgagcactag ctggcttgaa gatggaagag
    11761 atacaagcag aatcgtctaa tgaaaaaact gaagaatttt cagaattgct ataagaggtt
    11821 atgaaaatag tgttttgacc agagtattag agaaacaaaa cgaaaaaata gctccatgta
    11881 ttttaccgtt tttgaactaa tgtcgaagat tagctgattg aataccattt atcaagaagt
    11941 tatggaaaag ccggttcttt tttactaact gaaaaataaa attttattta gagatcaagc
    12001 tacagttgat aaatgatata aaaaagagaa tttcccatga aatagcgagg ttgtgacaga
    12061 agtgggcagc ttcaagaaat aagaaggaat ttacgaaaat tgcttttcaa atttttgtga
    12121 aatttcggct tatttttcga agaagttact tctggaacac cgtgtatcag aggttgcgaa
    12181 aaagctgccc tgcatcaagt aataagaaca gccaaacaaa aatagttcct catattttac
    12241 gtttggttga gcttattacc gcagatgcaa gcttttgaac accgttttat ttgtctcaca
    12301 cccttttcct tcctacttgt aaccttgctc ccgtgttaga aacatgttac actaaatatg
    12361 tgaaattatt aacaaggagc gtcgttatgc gagaaaaaac agatacactg ccaaaagcaa
    12421 ctgcgaaaag attaccttta tatttacgct acttgaagat gctggatgac tcaggtataa
    12481 gtagaatcaa atcaaatgag ttcagcgaaa tcacccaaat tccttctgca acgatccgca
    12541 gagacttctc gcaattaggc gagctaggca gaagcggata tggctatgat gtcccttttc
    12601 ttatcgatgt attcaataat atccttaata ctaaagaaga aaaacgaatc gcattagtag
    12661 gatatggaaa tcttggcaaa gcattgaaac acaacaattt tagaagaaat gaaaatctaa
    12721 atatcgtgtg cgtctttgat aatgatccag cactgatcaa tcgagtaatt gatggagaaa
    12781 tgatctatcc gattgatcgt ttcgcggaga tagccaaagc aaaaaatgtg acagtagcaa
    12841 tctctacggt accaagtaaa tattcacaat ctgctattga tgaaattgtc aaaggaaatg
    12901 taaccgctat acttaatttt gcgcctgaca gagtaactgt cccagcttat gtcaatgttc
    12961 aatacattga tttgacaacc gagttgcaga cgcttattta ctttgatgag aattactcag
    13021 aagttttttc ttaggaaaat ttgagtaggc ttattgatct tgcgagaact cgtcgtaatt
    13081 ctttcaaaaa ttttttagta ctaaatgaag tggttttttc acgaccactt gttgactaaa
    13141 aaaaaaaacc attgtagaat gaagcataac gctgaacaag aggagtaact ggcacattca
    13201 ttcttagaga gagaactatc tgctgaaagg ttctcaatga caaccagtga aggtagcttg
    13261 ggagtttctg tcttgaatgg agtagagata gacgagtcgt gatcgttaac tcacttgaga
    13321 ggcaataatg attgattatt gcaatttagg tggtaccgcg taaatcatac gtcctagaat
    13381 acgaaagtat tttaggacgt tttttagttt aaaggaggaa aacagcatga cggaagaaaa
    13441 taacctatca acaaaatata atccgcaaga agttgaagct ggacgttatc agcattggtt
    13501 agaacaagat ttgtttaaac cgagcggaga taaaaaagca aaaccttatt caatcgtaat
    13561 cccgccccca aatgttactg gaaaacttca tttagggcat gcttgggata cgaccttgca
    13621 agatatgctg attcgtcaaa agagaatgca aggatatgat actttatggc tgcctggtat
    13681 ggaccatgca ggaatcgcga cacaagcaaa agtagaagcg aagcttagag agcaagggat
    13741 ttctcgttat gatctaggtc gtgaaaagtt catcgatcaa gtgtgggaat ggaaagaaga
    13801 gtatgcgagc cacatccgcg aacagtgggc taaaatgggc ttatctttag attacagtag
    13861 ggaacgtttt acattagatg atggattatc tgaagcagta cgtaaagttt ttgttacctt
    13921 atacgaaaaa ggtttgattt atcgtggtga atatatcatc aactgggatc ctcaagcacg
    13981 aacagctcta tctgatatcg aagttattca taaagatatc gaaggcgcct tttatcatat
    14041 gagctatgaa ctagcagatg gatcaggcgt agtagaaatt gcgacaaccc gtccagaaac
    14101 gatgttggga gatacggcga ttgctgttca tccagaagac gagcgttatc aagcgctcat
    14161 cggcaaaaaa gtgattctac ctttagtcaa taaacaaatc ccaatcattg cagatgaata
    14221 tgtagatatg gaattcggga caggtgtagt aaaaatcact ccagcacatg atccaaacga
    14281 ctttgaggta ggtaatcgtc atgatcttcc acgcgtaaat gttatggatg atgatggtac
    14341 gatgaatgaa ctagcaggaa aatatgctgg aatggaacgt tttgctgctc gtaaagcaat
    14401 cgtgcaagat ctgaaagaaa tcggtcgatt gattaaaatc gatacgatga tccacagtgt
    14461 gggtcattcc gagcggacag gtgtcgttgt tgaacctcgg ctgtctactc aatggtttgt
    14521 aaaaatggcg ccgttagcga aaaaagccat tgataaccaa gaaacagatc aagcagtgga
    14581 attcttccct ccaagattca atcaaacgtt tttacgttgg atggaaaatg tccatgattg
    14641 ggtaatctca cgacaattat ggtggggaca tcaaattcct gcatggtatc acaaagaaac
    14701 aggtgaaatg tatgtaggga tggaagcacc agcggatagt gaaaactggg tacaagatcc
    14761 tgatgtttta gacacttggt ttagttcagc tttgtggcct ttctcaacaa tgggctggcc
    14821 agatacggaa agcgaagatt ataagcgata cttccctact agcactttag taaccggtta
    14881 tgacatcatt ttcttctggg taagcagaat gattttccaa agcttggaat ttactggcga
    14941 acgtccattt gagaatgtct tgattcatgg attgattcgt gacgaacaag ggcggaagat
    15001 gagtaaatca ttaggtaatg gaatcgatcc gatggaagta atcgaaaagt atggagcaga
    15061 tgctctccgc tggttcttat cgaatggttc tgcaccagga caagatgtcc gcttcagcta
    15121 tgaaaaaatg gatgcttctt ggaactttat caacaaaatc tggaatgctt cacgtttcgt
    15181 tatcatgaat atcgaaggaa tgaccgtaga agagatcgat ttatctggag aaaaatcagt
    15241 ggctgaccgg tggatcctta ctcgtttgaa tgaaacgatc gaacgagtaa ctgagctatt
    15301 tgatcgtttt gaatttggtg aagcaggtcg acaactgtat aactttatct gggatgattt
    15361 ttgtgactgg tacatcgaaa tgagtaaaga agtactttat ggagatgatg ctgtttcaaa
    15421 acaaaccacc cgtagcatct tagtccatac acttgatcag atcctacgtt tattgcatcc
    15481 aatcatgcca ttcgttacag aagaaatatg ggaacatatt cctcatcagg gaaattctct
    15541 agtagtggca gaatatcctg ttgtacgtcc agagtttgat gatgaaacag cttctaaagg
    15601 gatggaagta ttgaaagaat tgattcgagc cgttcgaaat attcgttcag aagtgaatac
    15661 accattgtct aaaccaatta cgttgctaat taaaacaaac gatgctaaaa tcgatcaatt
    15721 tttagttgaa aatacaagct atatcgaacg cttctgtaat cccgaagagt tgacgatatc
    15781 aagtgagatc actgcgccag atctagcgat gtcagctgtt ctgacaggtg cagaaatctt
    15841 cttgccgtta gcaggattga tcaatattga agaagaaatc aaacgattag aaaaagaact
    15901 ggaaaagtgg acaagcgaag taaaacgtgt ccaaggaaaa ctttctaatg aacgttttgt
    15961 ttctaacgcg ccggaagaag tagtagaggc agagcgtgca aaagaaaaag attatttaga
    16021 gaagcaaacg gctgtcaaag aacgaatcga aagtttgcgt actattcaat aagaatagaa
    16081 aaagagagtg tgaaatcagc tagctattac taaaagctag ctgattgaac atcgttacag
    16141 gaaagagttt gagcctcact cttagcttcg attcataagg agatgtgaca taaattttgt
    16201 cgcatctctt ttttagtcta ctcttattat gtaggagaag gagggaggtg gatcatgtat
    16261 caagagtcaa taaagtagtt gataaaatcg ttcctctctc tgtttctttt ctctttgtca
    16321 tacttttatt tttcttatgt gttctatttg ttataataaa agagaaatgg gggaattgtt
    16381 gatgacgatt gaagaagcaa tagaatggat ccatagtcga ttaccatttg gttcacgtcc
    16441 tggccttgac cgagtcgaag ctttattaga agtggtaggg catccagaga gaaaagtaaa
    16501 aacgatccat atcgctggaa caaacggaaa aggttctact gtagcttatt tgagaagcct
    16561 attagaagag gctgggctga cagtaggtac atttacttca ccttatatcg aaacgttcaa
    16621 tgaacggatc gcaatcaatg gcaaaagcat tccagacaat gatttgatcc agcttgtaaa
    16681 aaaatatcag ccactgatca aacaacttga tcaatttgaa gcagtagctg gtattactga
    16741 attcgagaca ttaacagcaa tggcatttga ttattttttg atgaagaaag tggacgtagc
    16801 gattatcgaa gttggcttag gcggcttatt ggattctacc aatgtagctg taccaatgct
    16861 gacgggaata acaacgattg gacttgatca taccgaaata cttggagata caattgaaga
    16921 aatcgctgca caaaaagctg gaatcatcaa acaaggcgtt ccagtagtaa caggaaatat
    16981 tgccccagaa gcttttactg tcatccaaca aacagctaaa gcaaaaaatg caccgcttta
    17041 tgtatacggg gaaaattata aaataaacta tctgcatcct gatgagatgt ggggagaagt
    17101 atttgaattt tttaatgaaa taggaaagat ccctcaatta accactccgc ttttagggca
    17161 acatcaagct gaaaatgcag gagtagccat cgaattgtat tttgtttact gtcaattgga
    17221 aaagctgcct ttccgagaaa aagatgtttt gaagggattg aagaaatctc gatggccagg
    17281 aagaatggag cgtttgagta acgagccatt agttgtttta gatggtgccc ataatacgca
    17341 tgcaatgaac cggcttgttg agaatttgaa aaaagaattc aaaggatatc aaatcaacat
    17401 tttgttttcc gccttacgaa ctaaaaatgt tagagaaatg ctagaacgat tgcagcaagt
    17461 accaaatgtc catatttatt tgacgacctt tgaacatcca aaagccatcg aattaagtga
    17521 agtatcagat ttagcaagtg agagagtatc aatcgtttca ttgtggcaat tcggattagc
    17581 agatatattg gaaaagatga caaatgaaga cgtattagtt gtgaccggtt cactttattt
    17641 catttcagaa gttcgccatt tattaataca attaggagga gatcatgaag tataatgcag
    17701 tgatttttga catggatggt ttgctgttcg acacagagtt agtgtattac gaagccacac
    17761 aagtagtagc agacaagatg ggttttcctt atgataaaga cctgtattta aaattcttag
    17821 gtgtttcaga tgaagaagta tgggcgaatt atcatatgat tttctcggaa tttggcaaaa
    17881 caagtgtaca aagattcata gatgattcat accaggaaac gcttgaacgt ttttcgcaag
    17941 gagcggtaca aatgaagccc ggtgtgttgg aactcctctc ttttttagaa gaacatcaga
    18001 ttcccaaagt tgtcgcttcc agtaaccaac gagcagtcat tgaactttta ttgaagaaga
    18061 acaatctgtt tgaacgattt gagacgattg tttctgcaga aaatgttcaa cgagcaaaac
    18121 cagacccaga aattttcttg ttagctaatc agaagctagg tacagcgaag aaagaaacat
    18181 tgattttaga agattcacaa aatggtattt tagcagcttc tgcggctgag atcccggtag
    18241 tcatggtacc agatctgctt ccaccatccc aagaattgat caaaaaaaca gctggggttg
    18301 tttcttcttt acatgaaatc cctgcattgt taaaataaaa atccatctct tttcgtatct
    18361 tattttagat aagatagaaa ggagatgttt ttttgggaaa acgattgatc aaagaagtac
    18421 ctactagctc tttaccaagg gaacgtatgg aaatttatgg cgcggaagct ttatcggatc
    18481 aggagctttt ggcaatctta ctgcgtaccg gtcaacaccc ttatagtgtg atgtcgattg
    18541 ctggaaatct gttaaaaacg tttggaggac tggcctcttt acgacaggct actttgcatg
    18601 agttagaaga gattcgaggg ataggacgcg tgaaagcaat cgaaatcaaa gccttgatcg
    18661 aattaggtcg acgaattcag acggatcatg aaagagcggc tccaccggtt cgtaccagct
    18721 acgaattggc ccagcaattg attctggaaa tgaaagatca caaacaagaa catttggttt
    18781 gtatttattt ggacacgaag aatcaagtga tcttaaaaaa aacagtgttc atcggttcgc
    18841 tgaatcagtc agtcgctcat cctagagaaa ttttccatta tgctgtacgt tattgtgcag
    18901 caaggatcgt tttagcgcat aatcatcctt ctggtaatgt tattccatcg cagcaggata
    18961 tgaattttac caaaaggata caaaagtgtg gagaaatgat ggggatcact gtactggacc
    19021 acttgatcat cggacgcaaa cgatatttta gtttgagaga agaaggaatg atggaagaga
    19081 agtaagttgc tttcttgcaa aaaaaatgaa gcaagtgtac aattatattg taattttgtt
    19141 tgggaataga agttgaaaca tctgttgcac ctctatcgta cacgagcaat gttacaattt
    19201 aaattagtgc ttttggcacg aggaggatat tattatggaa aacggaacag taaaatggtt
    19261 caacgctgaa aaaggatttg gatttatctc acgtgaagat ggaagcgacg tattcgttca
    19321 cttctcagct atccaaggtg acggattcaa aacattagaa gaaggtcaag cagtgacttt
    19381 cgacgttgaa gaatcagacc gcggcccaca agctgcaaac gttgtaaaag cttaatttta
    19441 tctgtaaatc aaattgatgg tagacatcaa tttttacgag agaacaaagc gagtaacaaa
    19501 aatacctgcc acaagagaca atttctcttg tggcaggtat ttttttgtat gaaaaagtaa
    19561 ataaatttaa gaggctatga aagagccacc ttgtatcaag gaataagaac aaacaaaaat
    19621 ggcttctcat gtttttcatt tgttttctct tatattcagg acaaggtgct gatttcataa
    19681 cctcttattt attcttcatc tggtgtcatt accattggca aaatcattgg atgtctttct
    19741 gtacgttcga ataagaaagg ctgaagcgcg ccagtcatcg cttctttcaa tttatattct
    19801 gaacagtcag gtccattcaa acattctctt aaagcgtgga ataagattcg ctgagcttca
    19861 ttgatcattt ctcctgattc tcgcatgtag acaaatccac gagagagaat atctggacca
    19921 gccaagattt cttttttctt gatatccact gtcgcgacag ctaatacaag accttcttct
    19981 gaaagcaggt gacggtcacg taaaacgaca ttaccaatat caccgatccc gctaccatcg
    20041 atatacacat ctccagccac aaaatgccca gcaggacgcg cagaatcgct agttaacgct
    20101 aaaacatcgc cattttccat gatgaagcag ttttctgcag gaacacctac atcttgtgct
    20161 agagaagcat gtactttcaa catgcggaat tctccgtgga caggaataaa atattttggc
    20221 ttcatcaaac gaagcattaa tttttgttct tcttgtccac cgtgaccgga agtatggatg
    20281 ttattgattt ttccgtgaat cacttctgca ccagcttcag acagcaaatt gatcaaatga
    20341 ttgacacttg ttgtattacc aggaataggt gaacttgaaa agatcactgt atcgtctggc
    20401 tggatttgga tttgacggtg agtaccattc gcgatgcggc tcaacgcggc catcgcttct
    20461 ccttgagaac ctgtacatag gatcatcgtt tcattggcag gcaagcgatt gatttcgtgt
    20521 gcatctacaa atgtgccgtc tggaactttg atatatccta aacgttgacc gttgacgatg
    20581 gcattttcca tgcttcgtcc gaaaacagcg attttacgac cagttgccac agcggcatct
    20641 gctgcttgct gcaaacggaa gatgttagaa gcaaagctgg caaaaatgat acgtccttcg
    20701 atttttgaaa agatcttcaa aatagaatgc ccaatcgttt tttctgattt agtaaatgtt
    20761 ggaatctcag catttgtact atcagataaa aggcacaaga ctccttcttc cccgatacga
    20821 gccattttat gcaagtttgc tggttcgcca acaggagtga aatcgaattt gaagtctcca
    20881 gtcgctacga tatttccaga aggtgttttc accacgatcc ctaatgcatc tgggatacta
    20941 tgggtcgttc tgaaaaagct gacagaagtc tttctaaaac ggatgatcgt atcttcattg
    21001 atctcatgga gctctgcatc tcgaagcaag ccatgctcat ctaatttgtt tgtgatcaac
    21061 gctaatgcga gaggaccagc atagatagga atatttgctt gtttcagcaa gtagggtatt
    21121 ccgccgatgt ggtcttcgtg cccgtgagta atcaccaagg cttttacttt gtgtaagttt
    21181 tgtatgatgt agctgtagtc gggaatgaca tagtcaattc caagtaaatc gtcttccggg
    21241 aatttgatcc cggcatcaat caaaatgatt tcatcttgga attgtacccc atacgtattt
    21301 ttcccgattt cacctaatcc accaatagca aagacgcctg tctcgttatt tttgatggaa
    21361 agtttcatat taaaactcca ctaatgagaa atcagcattt tctttttcgt actctagatg
    21421 acttccttct agaagttgaa cgaattcaat attgtatggg gtattttctt ccacacgttg
    21481 gcgagcgtct acatcgcttt ttgcctcaat gtaaagagat tttgtttctt cccgtttagg
    21541 gttgcgtact tttgtttctt gataataaac tttatagatc attgttttgt ctcctttatc
    21601 tgcgtaaagc agtgatttag ctagttgatt tttttagaca acacaaaacg aggggacaag
    21661 ccacctggtt tttcatatgc tttgactaga atactggatt cgagtcaaag agacaagcct
    21721 cgtttttgct tatctgttcg gatatttttt caaccgtacc ttctgtataa aaaatataca
    21781 acaattaatt gttattctag cataaaacag caaataagta tagaaatcaa ctagattgaa
    21841 agaaaaaatc aatgtttatc atcccgctat aatcataatg acttttttat gagagaagtg
    21901 ttctattaat aggctcgttg tgctatcatt tttagtaatg aaaatgtaac aaaagattaa
    21961 aaggagatga gaaagtgaaa ctaatgtggc attataccat gcgttataag aaattcttgt
    22021 ttttcaattt catatgcgta tttggtttta ttttgattga attaggactt cctacgattt
    22081 tagcacgtat gatcgatgta ggaatccgca atgatgattt tgattatgtg aagcaacaag
    22141 ggatatttat gatcgttatt actgtaatcg gtattatttt gaatatcttg ttaggttatt
    22201 ttggttcgag gattacgact aatatcgtag cagatattcg agatgatttg ttcgaaaaga
    22261 tccaaagtta ttcgcatcag gaatacgaaa atctaggcgt atcttcattg atcactcgta
    22321 caacaaacga tgcttatcag atcatgttgt tcctgcaaaa tattttacgt atcggtttta
    22381 tggcaccaat gatgttcatt gtcagtttgt acatggtcat gcgtacaagt gtgacactca
    22441 gtttgtatgt agtaggtgca ttacctttac tattattggc agtcgtagcg attgctaagg
    22501 tgtctgaacc gttatctaaa aagcaacaaa aaaacttaga taaaatcaac agtatcttgc
    22561 gggaaaatct ttcaggattg cgtgtgattc gtgcattcgt caatgaaaag tttgaagaaa
    22621 aacgttttgc tgtagtcaat gagaattatg cttctagttc aaaaagctta ttccgtttga
    22681 tggctgttgc acaaccgggt ttttatttct tatttaatat cgtaatggta ttgatcatct
    22741 ggagcggggc agtgcaaatc gatgcaggcg agcttcgtgt aggagatttg attgcgttta
    22801 tcgagtatat attccatgcc ttgttctcat tcatgttatt tgctagtgtg ttcatgatgt
    22861 atccaagagc agctgtttca gctggacgta tccaagaagc cctagatgcc aaaccttcga
    22921 ttgtagaaga tccaaatggt atatctgata caaaaactaa agggtatatc gaatttcaaa
    22981 atgtaacatt tgcttatcca ggccattcgc aagagccagt gatacgaaat gtcagtttta
    23041 cagcctctcc aggagagacc gttgctttta ttggaagtac cggtagcgga aaatctacgt
    23101 tgatccaatt gatccctcgc ttttatgatg tgagtgaagg aaagatcttg atcgatggag
    23161 tagacgtgag agattatcgg ctaagcaagt tgcgagataa gattgggttt attccgcaaa
    23221 aagcgctgct atttactgga acgattgcag ataatttgcg atacgggaaa gaagatgcta
    23281 cgcaagaaga aatggaacgt gcagctgaga ttgctcaagc ttcagaattc atttctcgaa
    23341 aaccagatgg atttaatgaa catttatcag aaggtggttc caatttttca ggtggacaaa
    23401 aacaacgatt ggcaatagcc agagccatca tacgccgtcc ggaaatctat atttttgatg
    23461 acagcttttc tgcattagat tatcaaacgg atgcgaagtt acgtgctcgt ctgaaaaaag
    23521 aaacgacaga atctactgta ttgatcgtag cacaacgtgt gggaacgatc atgcatgcag
    23581 ataagatcat cgtattaaat gaaggagaag tcgtcggtat ggggacgcat cgggagttgt
    23641 tggaaaaatg cccgatttat tatgatattg ccgcttctca attatcaaag gaggaattag
    23701 catgaaaaaa accttttctt ccttaaaacg tttagctcga tatatcagac cgtatcgtga
    23761 gacttttctt ttagtgatcc tatttacagt tctgacagtg gctttcaacg tggctttgcc
    23821 atatgttaca ggacttccta ctacagaaat cagccgtaat attgccaatg gcgaatcaat
    23881 caatttttct tatatattca aatgtctcat ttggatcaca gttgttggaa tagggtattg
    23941 tatttcgcaa ttactttctg gtgttttgat gacaaatgtc gttcaaagtg ccatgcatga
    24001 tttgagaaga gacatagaag aaaaaatcaa tcgtcttccg gtttcatatt tcgataaaaa
    24061 ccagcaagga aatattttgt ctcgtgtgac gaatgatgta gatgcagtca gcggtgcttt
    24121 acaacaagct tttattggta tcgtaaatgc gatattggga attacgttgg ctgcagcaat
    24181 gatgttttat atccagccga tgatggcgct gatctctatg atcatgattc caacatcgat
    24241 ctggatctca aaacgagtga tcaatgcttc gcagaaatat tttcaaagca tgcagaattc
    24301 tcttggtgag ctaaacggct acgtccaaga aaatatgaca ggttttagtg tattgaaagt
    24361 atatggacgc gaaaaagaaa cattggaagg attcaaaaaa gttaatcaca gcttgaaata
    24421 ctttgggttt cgtgcagcat tcatttctgg cttgatgatg cctctagttc aattaactgc
    24481 ctatgctact tatattggta tggctgttct aggaagcttt tatgttatta ctggggcgat
    24541 cgttgtcggt caactacaag cattcatcca atatatttgg caaatcagtc agccaatggg
    24601 gaacgtgaca caattgtctt cagttttgca aagtgcatca gcggcaacga aacgtgtatt
    24661 cgagatttta gatgagccag aagaaaaaga aaacaaagta gatgcttcac tgccagaaac
    24721 gattttagga gatgtgacat tcgatcatgt cgattttgca tatgatccga agaaaccatt
    24781 aatccaagat ttgaattttg aagtgaaagc tggtcaaacc gtagctgtgg tcggtccgac
    24841 cggtgccggc aagacgacat tgatcaatct attgatgcgt ttttatgacg tagacaaagg
    24901 agcaataagg attgatggaa tcgacaccaa atcaatgagt cgcagtgatg tgcgttcgct
    24961 atttggtatg gtcttgcaag atgcatggct ttatcaagga acgattgcag ataatatccg
    25021 cttcggaaag cttgatgcga cagattatga agtagttgat gctgcgaaaa cagcaaacgt
    25081 agatcatttt atccgaacga tgccagatgg atatgatatg gccatcaatt ctgagggaga
    25141 caatgtgtct ttaggacaaa aacagttgct gacgattgcc cgagcagtgg tctccgatcc
    25201 taaaatcttg attttagatg aagcaacaag ttcagtagac acccgtttag aagcgttgat
    25261 tcaaaaagca atggatcgcg tgatggaggg acggacaagt ttcgtcatcg ctcaccgttt
    25321 atcgacaatc cgtgatgcag atctgattct agtgatggat caaggtcaga tcattgaaaa
    25381 aggaacgcat gagagtttat tggcacaagg tggattttat gaaaaactgt ataacagcca
    25441 atttgctgag gaagcagaat aagtcatata agaataaaac agtttaaaac ttattttttg
    25501 ttctctattc ttgataatgg taaagtaaag ttatcaagaa aagtgaggac acggagatgg
    25561 aacagttttt aaatgaaaaa attactgaag ccaaaagaga agttgatcgt ttaacgcacg
    25621 tctgtcagac gaagcctttt gattcagctg atttttttga aaatgagttt caaatcgaac
    25681 gcttggcagc acaaatagaa gtgtacgaat taattttaaa aaaagtaata gaagaaaata
    25741 tagatgtaca ataaaaacag gtaaaacgtc agttaccaga tactgtaaat agacatctgg
    25801 cctgagcagg ttttacctgt tttttttctt atgaaataaa tatatgctat gataccttta
    25861 ttcaatagga ttagtttcta caaaaaatga aaggagaaaa ataaatatgg aacggtttat
    25921 agcatcaaat gccacagtga ttggtgatgt gacattgtca gaagatgtga cgatctggta
    25981 tcaagctgtt ttacgcgggg acagtaattg gataaaaata ggacaaagaa cgaatatcca
    26041 agatggtaca atcatccatg tcgatcatga tgcacctgta gatattgcgg agaatgtgac
    26101 ggtaggacat cagtgcatgc tacatggctg tacaatcgaa aaaggggcgc tgatcgggat
    26161 ggggaccacg atattgaatc atgcggttat tggtgagaat agtttgattg gtgctggatc
    26221 attagtaaca gaaggaaaag ttattcctcc taatgtcttg gcttttggtc gcccagctaa
    26281 agtgatccgt cctttgacaa aagaagaaat acaaaaaaac aaagaaaaca tccagcatta
    26341 tgtcgagata ggtcggatgc atgcgcaagg aaaattcaag gagcaaagat aggcagtagc
    26401 atccataaaa taaaaaagga tggtcagttt gagttgacca tcctttttta tttggtttag
    26461 ataagagagc ttaaaaaagc ttcctttgaa acagattaat ttaatgaaga actgttttcc
    26521 atgacttcat cgatcaatcc atatgctttt gcttgttcag ctgtcatata gttgtcacga
    26581 tctgtatctt tttcaatcac ttcaagcggt tgtccagtgc gttcagctaa aattttgttt
    26641 aagcgttcgc gtgtttgcaa aatatggcgt gccgcaattt cgatttcagt tgcttgccct
    26701 tgagcgccgc caagaggttg atggatcatg atctcagcat ttggtaaagc aaatcgttta
    26761 ccttttgtac cagctgtcag caagaagctt cccattgaag cagccatacc cataacaatt
    26821 gtttggacat ctgctttgac aaagttcatt gtgtcgtaaa tagccatacc tgctgtaact
    26881 gaaccaccag gagaattgat ataaagatag atatcttttt cagaatcttg agcgtctaag
    26941 aaaagcaatt gggcgatgat cgaattagca agatcatctg ttacttggcc gcttaacatg
    27001 atgatacggt ctttcaataa acgtgagtag atatcgtaag ctctttcacc tcgtgaagat
    27061 tgttcaatga ctgtaggaat taaattcata atagagttcc tccttgattt cgtttagaaa
    27121 tagtgtacca taatttagtt tctaatacct aaaatgtagt atacattttt ggtcaaaaaa
    27181 ggtcaaataa aaaacttagg caaattagtc gcataaaccc gctttatggt atgataagca
    27241 gagaaacaaa acaaggagga acaacagatt gatagttggt attactggcc ttatcggagt
    27301 gctgacaggt gctgtagttg tctatatcgg catgatactg caaatgcgtc acttggaaaa
    27361 taaagaacag cgtagacgtg aattggaatt gaaagtcaaa gaaatcgaga cgctgaatca
    27421 attaaataaa aagatcaacg agatcttgca aaaacggaca acgctgatgg aagaatatgt
    27481 cagctttgat gcatttgatg attgttatat cacgattgac gattttgcgt atctccaatc
    27541 attttcagcg caaaatagtt tttatctgcc aaattatatt ttagacgagc tatttaagaa
    27601 tatctcccat cgaaaagtga ttttatctcc agatgaaaca atgaagattg gtggatatgc
    27661 ttataaaggc ggacgcgtga ttttagagaa ttttacggat agtctgactg aaatgatcaa
    27721 tgaaaaaaaa gctcagttga agactatgac gcgccagccg attggtttct tctcgaaaga
    27781 tgatgtttgg agctgatatt ttgaagcatt gccgttgcac atgagagcgt ggtaatgctt
    27841 tctttttttc ttaatgatac aatagaactg aacggagtga agaaagatgt tacaaatcta
    27901 tgcccatcgc ggaagcagcg gcacccaccc agaaaatacg cttcctgcat ttgcagaagc
    27961 tgtacgagta ggtgcagatg gtatcgaact agatgtccat ctaagcaaag atggttattt
    28021 gatcgttatg cacgacgaag aagtggaccg gacgactaac ggtaaagggc tgattcgaga
    28081 aaaaacattg gaagaactca aaaaattgaa tgcagggggc aaattcagca aagaatttcc
    28141 agcagctaaa gttcctgaat tgagagaagt gttaggactg ctcctccaaa aaaattaccg
    28201 agggatgcta atgattgaaa ttaaaactga tcagtatgaa tatcccggta tcgaagaaaa
    28261 attagccgaa acattaaaaa gtagagattg gccttttcaa catgcatatt gcagctttca
    28321 tttggattct ttggaaaaaa tcgcattact ggaaccggag atacagctgg acttattgat
    28381 gagtacttct gataaaaaga tccaactagc agcagaaacc ccttatatag aaggagtaca
    28441 tccaaaaaat acttggttgt tcgaacatcc agtccaggca gctgattttc caaaagcaat
    28501 tcgtgcatgg acgattaacg atgaagaaga aatggccaaa tgtcaagaac tcaaaataga
    28561 tggaataata actgattttc cggagaaagc tcttcggtgg aaacaatcaa cgaaaagtag
    28621 gtaaaatatg gaaaaagtat tagtgatcgt cggtccgaca gcagtcggta aaacagcgct
    28681 aagtgtggaa ctcgcacaac tatttggagg agagatcatc agtggtgatt cattgcaaat
    28741 ctataaaaat ctggatatcg gtacagcaaa aatcaaagaa gaagagatga ataatgttcc
    28801 gcatcatttg attgatgtga tcgaacctac tcaacgttac tcagccgctg attttcagca
    28861 ttcaggtcga caattgatta ccgagattgc gaaccgaggg cacctaccta tcatagctgg
    28921 tggtacgggt ctctatatcc aatcccttct atatgattat cagttaggag cgacagaaga
    28981 agcgaatcct gagattagaa aaaagtatga aacgttagct gaaacaattg gttcccaagc
    29041 attatggaat cagttgaaag aaaaagaccc ggcagctgct gagaaaatcc actggaacaa
    29101 tcaacgcaaa gtgataagag ctctggaagt tttagagacg acaggacata gtatcacggc
    29161 acctaaagaa gagccaaagc aattgtatga ttacctcatg attggattag atacgaagcg
    29221 tagtattctt tatgaacgaa tcaatcaaag agtcgatttg atgctagcag aaggtttagt
    29281 agcagaagcg cgtaaggtgt atgagcttgg agaagtccag gccagtcaag gtattggata
    29341 taaagaattt ttcccttatt ttgaagggaa agaaacattg gaagaagcaa cagaacaagt
    29401 caaactacat tcgagaagat acgcgaaacg tcagttgacg tggtttcgta accgtttgga
    29461 tgcacagtgg tttgatctta taagtgagcc aaatcaaaaa agtcagattg agcaaacaat
    29521 caaaaaatgg ctggaggcgg aataaaatgg aagaaaaagt gatcatcgta ggggtagaaa
    29581 ccgaagcaaa ccagcgttac ttttcagagt caatggaaga gctagttcag ctgacgaata
    29641 ccgcatcagg agaagtcgta ttcacaatta cacaaaagag accacaagtg gatcggcaaa
    29701 cgattatcgg taaagggaaa ttacaagaat tagttcaaca agcggatgcg catgaagcgg
    29761 atttgatcat ctttaatcat gagctaactc cgagacagag ccaactgatt accgatgctg
    29821 ttggtcttcc ggtaattgat cgcgtgcagc ttatcttaga tatttttgcg atgcgtgctc
    29881 gttcgaaaga agggaagctg caggttgaac ttgctcagtt agaatacctg ctgcctcgtt
    29941 tagtagggca agggaaaaca ttatctcgtc ttggcggtgg gatcggaaca agagggccag
    30001 gtgaaacaaa gttagaaaca gaccgccgac atatacggaa taaaattttt gctgttaaga
    30061 aagaattgaa agaagtagaa gctcatcgag agcgcaatcg ccaaaaaaga aaaaatagcg
    30121 agatttttca gattggactt attggctaca cgaatgccgg gaagtcaact atcttgaatc
    30181 tattgacaca agcggatact tattcgaaag atcaattgtt tgcaacgtta gatccattga
    30241 ctaagagatg gcgttttgct gaaggatttg agattactgt aacggatacc gtcgggttca
    30301 tccaagacct gcctacgcaa ctgatcgatg ctttccattc tacattagaa gaaagtcaaa
    30361 atatggatct tttgctacat gtagtcgatg ctagttctcc agatcgtatt ttacaggagc
    30421 agactgtctt aaaattgatg gatgaactga atatgaaaga aatgccgata ctgactgttt
    30481 acaataaggc agaccagatc gacccagcga tgttcacgcc tacgctattc ccaaatgtat
    30541 tgatttctac tcagacgaaa gaaggtaaga tggcgttgat cgaagcaatc aaacgtcagc
    30601 tgatggaact aatgacccct tatacacttt ctgtgccaag caacgaagga cagaaattaa
    30661 gcgagttgag aagacaaaca atggttttgg acgaagcgta catggaagaa acaaacgaat
    30721 atgaagttaa agggtttgca aaaaccaatt caaaatggtt aagaaaaaac caataaaatt
    30781 ttattttttt aa
//
LOCUS       DASHRQ010000030        30348 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.030, whole genome shotgun sequence.
ACCESSION   DASHRQ010000030 DASHRQ010000000
VERSION     DASHRQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.030"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            169..513
                     /locus_tag="U2M88_001473"
     CDS             169..513
                     /locus_tag="U2M88_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEM8373862.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            514..933
                     /locus_tag="U2M88_001474"
                     /pseudo
     CDS             514..933
                     /locus_tag="U2M88_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1025..1375)
                     /locus_tag="U2M88_001475"
     CDS             complement(1025..1375)
                     /locus_tag="U2M88_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373863.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1341..2672)
                     /locus_tag="U2M88_001476"
     CDS             complement(1341..2672)
                     /locus_tag="U2M88_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEM8373864.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2928..3494
                     /locus_tag="U2M88_001477"
     CDS             2928..3494
                     /locus_tag="U2M88_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM8373865.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3639..3726
                     /locus_tag="U2M88_001478"
     tRNA            3639..3726
                     /locus_tag="U2M88_001478"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3671..3673,aa:Ser,seq:cga)
     gene            4060..5034
                     /gene="bsh"
                     /locus_tag="U2M88_001479"
     CDS             4060..5034
                     /gene="bsh"
                     /locus_tag="U2M88_001479"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEM8373866.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5359..7254
                     /gene="asnB"
                     /locus_tag="U2M88_001480"
     CDS             5359..7254
                     /gene="asnB"
                     /locus_tag="U2M88_001480"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEM8373867.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEVIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7449..7895)
                     /locus_tag="U2M88_001481"
     CDS             complement(7449..7895)
                     /locus_tag="U2M88_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEM8373868.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8123..8272
                     /gene="rpmG"
                     /locus_tag="U2M88_001482"
     CDS             8123..8272
                     /gene="rpmG"
                     /locus_tag="U2M88_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEM8373869.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8360..8878
                     /locus_tag="U2M88_001483"
     CDS             8360..8878
                     /locus_tag="U2M88_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEM8373870.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8955..9911)
                     /locus_tag="U2M88_001484"
     CDS             complement(8955..9911)
                     /locus_tag="U2M88_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM8373871.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10077..10883
                     /locus_tag="U2M88_001485"
     CDS             10077..10883
                     /locus_tag="U2M88_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8373872.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10880..11869
                     /locus_tag="U2M88_001486"
     CDS             10880..11869
                     /locus_tag="U2M88_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEM8373873.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11866..12870
                     /locus_tag="U2M88_001487"
     CDS             11866..12870
                     /locus_tag="U2M88_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEM8373874.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13002..13544
                     /locus_tag="U2M88_001488"
     CDS             13002..13544
                     /locus_tag="U2M88_001488"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEM8373875.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13564..14262
                     /locus_tag="U2M88_001489"
     CDS             13564..14262
                     /locus_tag="U2M88_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEM8373876.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14283..14495
                     /locus_tag="U2M88_001490"
     CDS             14283..14495
                     /locus_tag="U2M88_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEM8373877.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14495..15457
                     /locus_tag="U2M88_001491"
     CDS             14495..15457
                     /locus_tag="U2M88_001491"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEM8373878.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15475..15870
                     /locus_tag="U2M88_001492"
     CDS             15475..15870
                     /locus_tag="U2M88_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEM8373879.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16033..16398
                     /locus_tag="U2M88_001493"
     CDS             16033..16398
                     /locus_tag="U2M88_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEM8373880.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16395..18206
                     /locus_tag="U2M88_001494"
     CDS             16395..18206
                     /locus_tag="U2M88_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEM8373881.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18268..18435)
                     /locus_tag="U2M88_001495"
     CDS             complement(18268..18435)
                     /locus_tag="U2M88_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEM8373882.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEATIEEN
                     RKKAMRKRVSR"
     gene            18683..19672
                     /gene="galE"
                     /locus_tag="U2M88_001496"
     CDS             18683..19672
                     /gene="galE"
                     /locus_tag="U2M88_001496"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEM8373883.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19684..21168
                     /gene="galT"
                     /locus_tag="U2M88_001497"
     CDS             19684..21168
                     /gene="galT"
                     /locus_tag="U2M88_001497"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEM8373884.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21192..22172
                     /locus_tag="U2M88_001498"
     CDS             21192..22172
                     /locus_tag="U2M88_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8373885.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22363..23085
                     /gene="tsaB"
                     /locus_tag="U2M88_001499"
     CDS             22363..23085
                     /gene="tsaB"
                     /locus_tag="U2M88_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEM8373886.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            23069..23611
                     /gene="rimI"
                     /locus_tag="U2M88_001500"
     CDS             23069..23611
                     /gene="rimI"
                     /locus_tag="U2M88_001500"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEM8373887.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23618..24082
                     /gene="rimI"
                     /locus_tag="U2M88_001501"
     CDS             23618..24082
                     /gene="rimI"
                     /locus_tag="U2M88_001501"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEM8373888.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24079..25095
                     /gene="tsaD"
                     /locus_tag="U2M88_001502"
     CDS             24079..25095
                     /gene="tsaD"
                     /locus_tag="U2M88_001502"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEM8373889.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(25704..26405)
                     /locus_tag="U2M88_001503"
     CDS             complement(25704..26405)
                     /locus_tag="U2M88_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEM8373890.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            26866..28095
                     /gene="arcA"
                     /locus_tag="U2M88_001504"
     CDS             26866..28095
                     /gene="arcA"
                     /locus_tag="U2M88_001504"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEM8373891.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            28186..29205
                     /gene="argF"
                     /locus_tag="U2M88_001505"
     CDS             28186..29205
                     /gene="argF"
                     /locus_tag="U2M88_001505"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEM8373892.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRI
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            29320..30267
                     /gene="arcC"
                     /locus_tag="U2M88_001506"
     CDS             29320..30267
                     /gene="arcC"
                     /locus_tag="U2M88_001506"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEM8373893.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
BASE COUNT         9919 a         5197 c         6318 g         8914 t
ORIGIN      
        1 aaaaaagcgt tagcaactat gcttaaaaag catatcgact gaataaacta agcattggac
       61 aggctgaaag actacattta aaatgaaatg cgaagaaaca atagaaaata aagttgcctt
      121 agaaccatag ttgaatcacc taacagtcgt gaagggagaa taagagaaat ggcagaaaaa
      181 caaaccgcag gacgagatca attaggagat tttgcaccaa aatttgcaga attgaatgat
      241 gatgttttat ttggacaagt atggtccaga gaagaattat ctgctcatca gcgtagtatc
      301 atcaccatat ctgcattaat ttcaggagga aattttgaac aattgccagc tcatctgaag
      361 attggcaagc aaaatggcat tactagtgaa gaaatagcag aaatcattac ccatttagct
      421 ttttatgtag gatggccaaa agcttggtca gcttttagtt tagcaaaaga aatctattta
      481 gaagaaaata gcgaagataa gaaagaggcg taaatgacga caaaacacga agaaatacac
      541 aatggcgtga tttttccagc aggtgaaaaa aacgaagctt acgctcaagt atttaccggg
      601 taaagctatc tgaatatgtt agtgaatgat cccgaagttt ctgttggtgt aggtaatgta
      661 acttttgaac caggttgtcg caatcactgg catgtccata aagacggcta tcaaatctta
      721 cttgtgaccg gaggagaagg ctggtatcag gaagaaggaa agccagcaag acgattaatg
      781 gctggagatg tcgtcgtaac gcacaaaggg attaaacatt ggcatggtgc gacaaaagac
      841 agttggtttg aacatttggc gatcactgct ggtaattcag agtttttaga acctgtaaca
      901 gatgaagtgt atgaaggatt ggaaaatcaa taatgtcaag cttgaacagc tcctgaaagt
      961 agattttaat cgaactttca ggagctgctt ttttgattca gtcttttata aatagaaaat
     1021 agggttaata ataggaatga gaagcacgtc gaaagatctt aatattgata atcggctggg
     1081 catacatgat ttccacgtca tcaatcaaac tacctaacag atgataatct tcccgatcat
     1141 gtcggggatt acctaatagt tcttgataat agatttccat actagggtcc cgaggattgt
     1201 tcagcaaatc agcaagtaca tctaaatctt ctggctgagt ttctgccgta ccctgtatct
     1261 ctatgaaaag cccttcctca gcaaattcta ctccaatacg tatttcagtt atttcatggg
     1321 aatataaata agtaagaagc tcatcgatga ttctttttga taattctttt tccaagtgtc
     1381 tttttttcct ttctgacagc ggtaaagtta tctagtatcg gtacaaggat acctgcaacg
     1441 aaaccagttg aaaaaccact gttatataga tttagtcctc cgtgcaaagt gctgacgtta
     1501 tggacaagag caatatgtag tacaccagct attacaccgt aaaaactgcc gtaaaaaccg
     1561 cttatgggcg cgagacctgt cccaaacaca gctgcaacaa gcatgccagt actggtgatc
     1621 tcatgaaaag ttccaaacaa agaagcaata aatattccga ctagtatcgg aaaactgttt
     1681 tttaaatgac aaccaaaagc actgaaaccc accgcagata aaatagcacc gatgacaggt
     1741 ccatttaact gaccgccaac cagcaaaaca taagaagaca gcatcagacc aactaagcct
     1801 atgttgatca aagtagcacc aataccagaa ttagcaatga aatcagttgt caattttcca
     1861 gatgaatcga aaatttctcg aatccccaaa agccgaaagg aattataata gaaaccaatt
     1921 ccgactgtca gcgaaaaaaa gccaaaaata atccaaatga gaggaaagtg atattcattg
     1981 aaaaccaatg tattttcgac gattgaatag ctgaataacc tcaaaacagc agtaaaagtc
     2041 atcgcaatca acccagaggt gaatccaata ttataaagtg tgaatccacg atggaaaact
     2101 aaagtttgag cagctaaggg cggaagaagt agaccaattg ctatgccagc taaattcccc
     2161 aataaaatac ctatcagtaa aggaaaacgc ataccaaaag tgatataact aacgaccggt
     2221 gataatgcgc taccaaacag tccgatcatg atgtactgag aaaaaggttt attgacaaat
     2281 acagcgtata gatacacacc taaaatgatt gagatagaat tataaaggtt tttgccaaaa
     2341 aaagagaaac cggtaactgt tagaatcgaa gcaaataagg gaccgtttaa aatcaccttg
     2401 tgccgataag ctaaaagcac agaaagtaag gtcaaaatcc ctacatttat gaatgcgctt
     2461 ccgaaaccgc ctaaagcaaa ataatctgtc aaaagattgc ttggcgaggt aagaatagtt
     2521 agcaatccac tccaattatc agtaggagta ttagaaagca agccaataaa aagaaaaaaa
     2581 acacctgtaa aaccaagata aaaatacttt cttttctgtg aaacgattcg tttttgagga
     2641 tgaatataaa taacgtcagt ttcgttttcc attacacacc ttctttttca ttttctttaa
     2701 ctataacttg aaatgaaaga tttggacaac tatcatttag gtcaaaacta aaaatgtaag
     2761 ggaacccatt gaatttaacc aaattttttt aattttgcaa aaaaaagtta aagaagactg
     2821 aaaaaaaatt taaaatataa cgttttgagt aaaatgtttg caaaaaatgc taaaaatagg
     2881 gtacaataaa acaacatata ctgcagtaaa gaagtggggg agttttaatg agaagaaaag
     2941 tctataccaa ggatcaaatt ctaaaagctg cttatgatgt tgttgcaaag gaagggttta
     3001 aaggatttac tgcgagaaat attgctaaaa agatggggat ttcgacacag ccgatttatc
     3061 tggaattcaa aaacatggaa gatttaaaaa atacgttgct ggattctatc tttgaacaac
     3121 taaaagaaga tgttttccct attgaacgta ctggtgataa aatcattgat ctcggtttga
     3181 actatatcta ctttgcaaaa aagcatcata gtctttacat ggcattgtac attgatgaac
     3241 atggggacgg aaagaaaatg catgattttt catacaatta tttccgtgaa atagttaaag
     3301 aagatgaaag atacaatcaa ttatcggatg aatatgtcga tgctctgcat actggtacat
     3361 gggtagcagt gactggctta gcgtcattga tgtcatcaaa catcatgcat ccaacagaag
     3421 aacaaatcat tgcactgatc aaacggacaa ttgactcgat tttagcatta gataatcctg
     3481 aaataatcag atagtgttct atcagtatga atttgatcgg taagtccgtt ggctgcaaac
     3541 tgtcaatgga cttttttgct aaaaaaagcg atttctgtta tttatctatt agaaataggc
     3601 ttgacatttc gtgaaaaaag tttaaaaata gtacttacgg aaggttggca gagtggtaat
     3661 gcaccggact cgaaatccgg cgaaccggct aataccggcg cgcaggttca aatcctgtac
     3721 cttccttata tcaacgatta gaggttgtga agaagctgtc ctgcatcaag taataagaac
     3781 ggccaaacaa aaatagcttt tcctattttt tcgtttgatt gggcttatta ccgcagatgc
     3841 aagcttttga acaccgtttg ttcggaataa ggggctgtga caagactttt gtcacagccc
     3901 cttattgttg tacttttaac ctacaatatt ttttgattcc ccctgagtac tattttttat
     3961 aaatgctagt aaatcaatct tctttggcct ttttttgata aaaaaaatac tagatgtaag
     4021 ataaaagtgt aaaaacgatt tgttttagga cgggaaacga tgtgtaccgc aatcacttat
     4081 gcaacgaaag accattattt tggaaggaat tttgattatg agatgtctta taatgaagtg
     4141 gtcactatta ctccaagaaa ttatcgtttc gattttagaa aggtaaaaaa tttagataaa
     4201 cattatgcaa tgataggaat agcagctggg gtatccaact atccactcta ttatgaggct
     4261 acaaatgaaa aagggttgag tatggcagga ctgaattttc ctggcaatgc tgattataaa
     4321 gaattacaag aaggaaaaga taacgttgca ccatttgagt ttattccatg gatactaggg
     4381 caatgctcaa ccatagacga agctaaagaa ttattggcta ctatcaatct agtcaatatc
     4441 gattttagtg aaaaactacc cttgtcacct ttacactggt tattagctga taaagaaaaa
     4501 tcgatagtaa ttgaaagtat gaaagatgga cttcatttat atgataatcc tgtcggtgtg
     4561 ttgacaaata atccgccatt tgattatcaa ttatttaatc tgaacaatta tcgctcgcta
     4621 tcaaatggaa caccagaaaa tcatttttca aaccagatca gcttgaatgt ttatagtcgt
     4681 gggatgggag gattgggctt acctggagat ctctcttctg tttctcgatt tgtaaaagcc
     4741 acatttacta aaatgaatgc tgcatcaggt gattcagaat ctgaaagtat cagtcaattt
     4801 tttcatatac taggctcagt cgagcaacaa aaaggagtat gcgatactgg agaaagaaaa
     4861 tacgaatata cgatttattc ttcttgctgt aacgttgata aagggattta ttattatcgt
     4921 acgtatgaag acagtcaaat tactgctgta aatatgaata aagaagactt ggatcgtcat
     4981 gaattggtca gttatccagt cataaaagag caacagataa actatgtcaa ttaattaatg
     5041 aatagatccg tttggtatca tattttctga tacagtcata attagacacg atctttaggt
     5101 cgtgtctttt tttgctggtt tttttggaaa tagcatccac taatattttt ttaacacgaa
     5161 gagaataagg tagcctcaaa aaagaaaaca tggtaaatta gatattggga ctttattttc
     5221 tgtaaaaaca gaaactttca tcttttgttt tcacgtgacg aaattgtaag agagaaaata
     5281 ttcgtccgct atttattcta tgataagatg catatgaatg aaataaacag taattttata
     5341 ttaggatgtg aaagttaaat gtgcggtatt gtaggttttg ttaacgacaa ggacaacaaa
     5401 aaaacaatta ttaatacaat gatggatcga atcgttcacc gtggacctaa tagttcagga
     5461 gaatatatcg ataaacatgt tgctttagga ttcagaagat taagtattat tgacttagag
     5521 ggcggtactc agcctattta caatgaagat cggacaaaga tcattatttt taatggcgaa
     5581 atctataatt accagccttt gagagaagaa ttgattgcag ctggccacgt ttttcaaaca
     5641 catgcagata cagaagtcct tttgcatggt tacgaagaat ggggaacaga attattgcaa
     5701 aagatccgcg gaatgtttgc ttttgctatt tgggataatg aaaagaatga gttattcggt
     5761 gcaagagacc actttgggat caaaccatat tattatgcag aaatgaacgg aacatttatg
     5821 tttggttcag aaatcaaaag ttttctgcct catcctgatt ttaacaaaga attgaacaaa
     5881 gaagcgttga aaccttatat gactttccaa tattctccat taaacggcga aacattcttc
     5941 aaagatgtct atcgtttgcc tgaaggccat tattatacgt ataaagatgg aaaaatagac
     6001 attcagcaat attgggatgc agattttgaa acaaaagaaa cgcattcccg acaagaatgg
     6061 atcgaaaaaa tcgacgaaac cgttcaagct tctattgaag cacatacggt cagtgatgta
     6121 gaagttggtt ctttcttatc cagcggggtt gattccagtt atgtaacctc tgtattgaaa
     6181 ccagatcatt ctttctcaat cgggtttgat gataaaacct ataatgaagt gattgaagcc
     6241 agaaaactaa ccgaactatt ggacttagat aatacagcag cagtgatcga cggagacatg
     6301 tcgttcaaag cattcccttt gatccagtat cacttagatg aacctgattc aaatccttct
     6361 tgcgtacctt tgtacttttt agcaaatctt gcttcacaaa gtgtgcgggt agtccaatct
     6421 ggagaagggg cggatgaact gtttgcggga taccaaacgt atggattcca tacgaattca
     6481 aaattcatcc gtgtgattgc tcaaggattg aaaaaattac caaaaggaac acgctataat
     6541 ttaggacgta aaatcggaaa aatgaaaaat ttccatggac ggatccattt atatgaatca
     6601 cttgctcctg caaaagaata ttttatcggt catgctcgag tgttcgaaga atcagaagca
     6661 tcagaagtgt taacaccaaa atatcaaaca gcaccatctg tggatgaaat catgactgtt
     6721 cattacgaga aaacagaagg aatcaaagat gaagtcaata agatgcaata tgtcgatctt
     6781 catcaatgga tgccaaaaga tatcttgtta aaggcagata aactttctat ggcaagttct
     6841 ttggaagtcc gagtaccttt attggatatc gaagtcatga aattggctca acaaatacca
     6901 agtaagtttt tattgaatca aaacaacaca aaagatattt tcagacaagc tgcgaacaaa
     6961 catcttccgg aaaaatggtc gaatcgtgtg aaattaggat tccctgtgcc aatcaaagct
     7021 tggttgaaag aagaacacgg ctacgaacaa gtcaaagcat tgttcgaagc agattttgca
     7081 aaagaatttt ttgatcaaga aaaaatcatg aagttgctag atgaccatca cgaaggacgt
     7141 aaagaagagc aaagaaaaat ctggacaatc ttcagtttct tgacatggta taaagtctac
     7201 tttgtagatg aaacgattcc gcaagcagaa gcaatcgact atgtgactgt ttgatgaaaa
     7261 tcaaatatca ataaaaaaga ggttttctcc taccgtttaa cactgagtga taagaaacaa
     7321 tttcaaaaaa cagctcttcc cgttttatag atattatgac ttatggttcg gagttggcag
     7381 aggaacacca ttgtttcgaa cataaggggg ttgtgacacg attttcgtca caaccccttt
     7441 acatatgttc aataatcgat ttctttttct tttgtcccat cttttttata aatgaacaaa
     7501 gttgattctt tgttttgtgc tacttgcttt cctttttcga ttgcttcttt ttttgtatca
     7561 aaatggttgc ttgccttttt tgccccttta gatttcacga tccattgttc ttcctcatat
     7621 tctacgatca catctgaatc aagcagtttt ccagcacgag gattcgtatc gtgttcatcc
     7681 gtcttgcttg gatttttttc tttttcgaaa gttcgttttt ctgattcact agcatcttca
     7741 taccattttt ttgcttgatc aatggcaatt gggatcgcac ggttatcagg gtacccttca
     7801 tctaataatg catttgcgat gtcgattgct ttttttcttg tcaatttatc taagtttttc
     7861 attgatgcag gaaagtcctt catgttccat ggcatattag tatccccctt tattaaaaaa
     7921 cataatctat ataaacaagt gtaagggtat ttatacaaaa agaaaaagat tttgtttgag
     7981 aagtaaaacc gttgtattta tcgtcaagta gttttactta acaaaatcgt gaaaaacact
     8041 agcaatctag aaaaaatcat ggtaagataa tcaagtctga gagtcatctt agaggattct
     8101 tgtaaaaatg ggagggaaaa acatgcgcgt aaacatcact ttagagtgta cttcttgtaa
     8161 agaacgtaac tatctaacaa gcaaaaacaa acgtaacaat cctgatcgtt tggaaaaaca
     8221 aaaatattgc ccacgcgaac gcaaagttac tttgcaccgt gaaacaaaat aatgttttta
     8281 aagttctcaa ttccttgctg gtcaaagaat tgagactttt tttatctcaa aacatctaaa
     8341 atcataagga gcggataata tgtttatttc catgtgggca caagataaaa atggtctgat
     8401 cggcaaaaat ggtttgttac catggcgatt gccaaatgat atgcgtttct ttcgcgaaca
     8461 tacaatggat cgaattttag tgatgggaag aaagacctat gaaggaatgg gggatctttc
     8521 acttccttat cgccatatca tcgtgttgac aacgcagcca aattttcgca cgaaagaaaa
     8581 tgcggaagta atgcattcga tcgatgaact tttgtcgtat agtcaaacga tctcagaaga
     8641 tatctatgtt tcgggtggta gtcggatttt tcaagaactt cttccttaca ctggtcggat
     8701 ctggcgaaca ttgatcgata gcacgtttga aggcgatacg tatattggga atattgattt
     8761 ttcagacttc gcccttatag aggagtatga ggggataacg gatcaagaaa atctatatgc
     8821 ccatcgtttt caaaaatggg aaagagtaaa aaatagggtt gcttcaaaag aagaataaaa
     8881 tctatgcgtc tagtttgtta ataagaaaaa aagcatatca cgactaatcg tgaagatgct
     8941 ttttttagtt aaaatcaatt tagtttttct ttgatatcat tgaccatttc ctgactggca
     9001 ataggtccgt tatatagcca actagattca ggtccgaatt gcagtacatg attgtctgtc
     9061 actgctttaa gattcttcca gcttggttca ttgaacatgg cagcatcttt gtcactgttc
     9121 acaaggatga tgtagtcagc atctaattca gctagctttt cagaggaaac agcagaccag
     9181 tcagaagtgg cttttttgga gacctcttcc actagatcag gaacaccaaa ttccagatcg
     9241 tcgtatacga ttcttccact tgaccgattt ttgctgacca taaaggctga gttgttggtc
     9301 acccacaaca ctgcagctgt cttgttctcg attttatctg ctagttcttc tcgagttttc
     9361 ttgatggatt tttgataatc ggaaatgatc ttttctgcct ctttttcttt tgccaaagct
     9421 ttgccgacat cttttagttg ttcttcccat gtgacatctg ttccgttttt tacaacataa
     9481 gttggcgcga ttttgctata ttgttcgtat ttcccgcctt cgacagtagc ggaagaagaa
     9541 atcaacagca aatcaggctc aaagcttaac actttctcat aaggaaggtc ataagaaatc
     9601 gttgggacat cctttaaatc atcttgcaga taatcttgaa tggaaccact gccaacagtc
     9661 cactgggcga taggtttttc ccctagtgca atcagatagt cttctaagta actgccgatg
     9721 atccgtttgg gcgcgtttgg aatctctact ttatgtccaa gtgtatctgt gactgtatga
     9781 gtactattgg atgaagtcgt cgaattggca gttgtagctg tttctttatc tgaagagggg
     9841 gagcaagcgc tcagtactcc agccagcagc aatatgctag ataatgtgaa tacttttgtt
     9901 tttaatttca tttctttcct cctaacataa ttgagaatct ttctcattgg ttattattcc
     9961 tgatatttcg aacgctgtca accgcttcat ttggctatag atatgaaagt agcgcttgac
    10021 gaaatgttga aacagcgtca taattgagaa tgattttcag cgaaaggatg ggtgaaatga
    10081 cgagattaag agcagctgac attgcagccg gctacgaaaa caaaaaaatc attgaaaaca
    10141 tgtctgttac gattcctgaa gggaaaatca ctagtttgat tgggccaaat ggaagtggaa
    10201 aatctacttt attgaaaagt tttacccgta tactacctgt gaaatcagga aagatcattg
    10261 tcaataactc tgatatctct acatacagtt caaaagaact tgcaaaaaaa attgcattat
    10321 tagcacaaac tagtgaacac cctacaggta tgacagtaga agaagtagta tcttacggac
    10381 gataccctta tcaaaaactg ttcagtggca tggacacgca agatcatgaa gcaatcgagt
    10441 gggcgttgga tgctacaggt ctagctggat ttcgtatgcg ggatatcgca acgctgtctg
    10501 gtggacaaac ccagcgtgtc tggatcgcta tggcgcttgc tcaggaaaca gatatcttga
    10561 tcttggatga accgacaaca tttttagatc cggcacacca attagagatc ctacagttat
    10621 tgactgaaat caatcaaatg aaacaaacaa cgatcttgat gtcgattcat gacattaatc
    10681 atgcttcacg tttttcagat tatttattag caatgaaaaa cggtaagttg atcacagaag
    10741 gtacacctga agaagtgatc acaaaagagt gtatgaaaaa aatatttgag attgatgcaa
    10801 cgattgtcac gcttccaaga acaaataaac cagtttttct aacctatgat ttgttgccaa
    10861 aagagcagga ggatcaatta tgaggactat gaagaaaaat agactcctgc ttttggtctg
    10921 ttttgttctt ttgattattt tttttgttgc tctttcaaat ggcgcagcga ctgtgggaaa
    10981 agcagatttt tggcaggcgt tgtttgattt tgatagatca aaacaaagtc accaaatcat
    11041 acgaaatatt cgtttgcctc gtgtggcggg agcatttctt gtagggagtt gttttgcatt
    11101 gagcggtgca ttgatgcaag gagtgacaag aaatccttta gcagattcag gcttactagg
    11161 aatcaacgcg ggtgcttcgt tggcaatggc ccttagtttt gctttttggc ctaaagcttc
    11221 tgatttttct gtgtttctaa tgtcattggc aggttctttt gctgtaactt tgctcgtatt
    11281 tggttttttg cagttttcga aaatgggcat gaatccttta cagctgattt tagctggcgt
    11341 agctattagt tcttttttta ctgctatcag ccaagcactt acccaattct ttaatctgca
    11401 gcaggatctt gctttttggt ttgtaggcgg agctgccaat atcacatgga aacaattagc
    11461 agtattgttt cctttatata tacttgctgt tttggtttct ttttcattag gaaaagcaat
    11521 atctttgatt catttgagtg aaacacatgc attagctctt ggaagtcatc ctaaacgaac
    11581 gcggttgatt gttttctgtc tagttgcagt gttagcagcc attgccgttt cgttagtcgg
    11641 acctgtttca tttattgggc tgttggttcc gccagttgtt cgcggaatgg ttggaacgga
    11701 ctatcgttat gttctacctg tctctttttt agttggcggt ggattggtta tgttggcgga
    11761 ttttacagcg aggatgatca atcccccttt tgaaacacct tttggattag tcattgcatt
    11821 gattggcgtt ccgtttttac tgatacaagt aaggagggaa caacggtgaa aaaaatatat
    11881 tggacgttct atttcttgct ttttgtactt gtcatcctgt ctataggtag tttaatgatt
    11941 ggtacgcctt ttatttacct ggatcagctt tttgccacgt tcatagggaa agaatcatct
    12001 tctttggcgc tgattgttag agaatttcga gttcctcgat tactgatcag tttattagca
    12061 ggagcttgtc ttggcgtttc tggttatatc ttgcaaggag taacaagaaa tgaattggca
    12121 gattctagta tattgggcat taatgccgga gcaggatttt tagtgatgct ttatttaggt
    12181 ttcttttcgc actattcgct gccgtttcta ttgctagttg tagcctttct aggtggtgtt
    12241 ttagctgctt gctgtgtcta tcttgcggcc tatgatcgaa atggcttttt gggtatgaat
    12301 cgaatcttat tgtccggtat tgccgttaat gccggccttt ctgcattaac attgctatgc
    12361 acgatccaat tatctaagga aaattatgga tttgtgaatg catggttagc gggatcaatc
    12421 tggggtgcga gctggcctta tgtttgggca ttgcttccat gggcggttat cttaattccg
    12481 ctaggaggac tttatgcaca gaggattgta ttgctatcat ttggtcaaga acgcgcgcaa
    12541 ggattaggcc ttaacgtggc taaaagtcaa aaaatccttt tgttgttagc tgttgctttg
    12601 gcaagcggta gtgtagcggt gacaggtagt ttatcttttg taggactact agctcctcat
    12661 gctgcgaagc tagtcgtacg taaagaaaat aactggacct ttgttctgag cggtttgttc
    12721 ggttccattt ttctgcttag tgcagatatg gttgctagag tgattctact aagtggggaa
    12781 gtacctacag gaattttgat tgcccttttt ggtgcgccat attttctgta tgcacttttt
    12841 atcagacaaa aacacgcgct gaacagttga aattcattaa aaaaggaagg ccaatcattt
    12901 ctaggtcttc tttttattct atttatgcat ttatttgaaa gttcgattgc tatttttctt
    12961 tttttcctat accattttat gagagaagga agggtgagtt tttggaccaa ttgaaagtaa
    13021 gattacgccg gaaaggaatc gaaaagctga aaaatctggc caaaagtggc aagaaaaagg
    13081 aaaaagaaca gcgaatctat aaacagctat tcgccagcga gtactggaag tcagcaaaag
    13141 tgatagggac gactttatcg aatgaatttg aactggatac acagccggtt atccaacgag
    13201 cagaaaaaga acaaaagaaa atagttgtac ctaaaacttt gccgaaacgt cagatggcgt
    13261 tttatgaaat ggatgaacag tccgttcttg tacgttcgag ttttggtgtt cttgagccac
    13321 aatcagagaa gctctatttg ccggagatga tcgatctcct cattgtacca ggtattatct
    13381 acaatagagc cggttatcga atcggttttg gcggtggtta ttacgaccgg tatttaaata
    13441 attttcataa aaaaacatgt agccttgttt ttcgtgaaca attgaatgaa caatggcaac
    13501 cggagagctt tgatcagaag atccaacaat tgtttattga ctaaaatgaa atgggaggtt
    13561 ttcatgaatt atcaacagca aataaaattg cgtgcatggt taagacgacc gttatggacc
    13621 tatgcttttt tagggataca gacagcggtc ttcctcatga tggaattatt tccgcgactt
    13681 gagatccctt attatgccgg aatgtatggt ccttatcttg ttcacttcaa tgaatggtgg
    13741 cgtttagtga caccaatctt catccatttt ggactgatgc atttcgtaat gaactcactg
    13801 atactgtatt ttatggggca gcaaatcgag gcaatctatg gccattggcg ttttttcttg
    13861 atttatatgt tcagcgggat aatgggaaat acagcaagct ttgcttttaa cgaagcgaat
    13921 gttctgtctg gtggtgcaag tacttcgata ttcggtttgt tcggtgcgtt gtttatctta
    13981 gggtttcatt ttaaacataa cccagcgatc cagcagctag tcagacattt tcttttattt
    14041 attgtgttga catttgtttt tggtcttctg gatacctctg tagatgtttg gggacatgtc
    14101 ggtgggctaa ttggcggatt agtgttaggg aatgttttgg gtctaccgaa acaacagaca
    14161 agctattcga tacaccaaag gattctttct acactagttt ttgtatttct ttttgtaata
    14221 tgtattttac tgggtttaaa aaaatatggt ttacttgtat aatatgaagg aagtgcttaa
    14281 agatggagag cttatatgat gtacagcagc tgttcaaaaa atttggcatc tttatctatg
    14341 tgggtgcacg catctatgac attgaattaa tgactatcga attgcgtaat ctgtatgata
    14401 gccgtttgat tgatcatgat acatacatga cagcgtggcg catattaaaa agagagcacc
    14461 gaattgaaga aagccgaaag aaaggaaatt tgtaatggac aagaagatta ttgggattga
    14521 tttaggtgga acaacagcaa aatttgcaat tttaacacca gaaggagaga ttcagcaaaa
    14581 atggagcatc gataccaata ttttggatga tggtaagcat atcgttcctg aaatcattga
    14641 atcaatcaat catcgtttga atttatataa tatgaaagcc gaagacttta tcggaatcgg
    14701 catgggtacc ccaggaagcg tggacagcga agctggaacg gttattggtg cgtataactt
    14761 aaactggaca gaagttcaat ttgtgaaaaa actgatcgag gcaggaacag gaatcaaatt
    14821 cgctatcgat aatgacgcga atgttgctgc tttaggtgaa cgatggaaag gtgctggaga
    14881 aaacgatcca gacgttgtat tcgttactct aggaacaggt gttggcggag gtatcgttgc
    14941 tggcggaaat ttgattcacg gcgtgagcgg agctggagga gaaatcggac atatcactgt
    15001 ggatcctgaa gggttcgaat gtacttgcgg taaacgcgga tgtttagaaa ctgtatctag
    15061 cgctacaggt gtggtacgcc taggtcgttt cttggcagaa gaatacgcag gggattcaaa
    15121 actgaaagca atgctagaca acggagaaga agtaacaagt aaagatatct ttgaaatggc
    15181 tcaagaagac gatccgtttg cattgatggt tgttgatcgc gtatgcttct acttaggttt
    15241 agcttgcgga aacttgggga atacattaaa tccgtcaagt atcgtgttag gtggaggagt
    15301 atcggcagca ggagaatttt tacgtagccg tgtagagaaa tattttaaag aatttacttt
    15361 cccgcaagta agagaatcaa caaaaatcaa attagcagag cttggtaatg aagctggtgt
    15421 cattggtgct gcctcattag ccttgaaatt tgcttaaaaa aacggaggca ttacatggtt
    15481 ttatgggtaa ttaatatcat tttgctattg attgtgcttg caatcatcct ttgggaagtc
    15541 tatctacgag taatggccaa acgttcagca acgactttga cagaagaaga atttcgagaa
    15601 ggaatgagaa aagcacaagt aatcgatgtt cgcgaaaaag atgtgtttga tgctgggcat
    15661 attttaggtg caagaaacat cccttacact gttttgaaag attcattagg atctattcga
    15721 aaagaccagc ctgtctatat ttacgatcaa aagaaaagtt tgagcatccg tacagcaaat
    15781 aaattacgta aagcaggtta tcaagagatt tatttactta agggtggata tgacggatgg
    15841 tctggaaaaa tcaaaagtaa aaaagtataa aacagatgat tgtatgacag ctttaaaaac
    15901 cgaatcaatc attagatttt gaccaagatc acatgaacgg cgcaatggat agttgtgccg
    15961 ttcatttttt ttattttgtg ctattatcta catatataag ctaagcgaaa aggtagaaag
    16021 aaggaaacaa ctatgatcaa attaaaacgt gtttaccttg atgctgaaaa agaggatggc
    16081 tatagaatac tcgtggatag attctggcca cgaggaattt caaaagaaaa agcgcagtta
    16141 gatctgtggt tgaaagaaat tgcacctagt aatgaagcac gcaaaacttt tgaccatgta
    16201 ccagaacgat ttgcttcatt tgcaaagaaa taccagcaag aattgatttt cgaagaagaa
    16261 aagaagcaag ctatttttaa actcagtcaa cttatgaaag aacatccagt cattactttt
    16321 gtttatggtg caaaaaatga acatcagaac catgctgttt tattgaaaaa atttatcgaa
    16381 atggaggcat gcaaatgaaa atagggatca ttggtgccgg acctagagga ttaagtgtag
    16441 cagaaaggat tctccataat aatcaggaca gacattctct tgaacttttt cttttcgatc
    16501 ccaatggtcc tggaggtcga gtttggcggt tgaatcagcc accggagtta ttaatgaatt
    16561 ctgtttctca acaagtgacg ctttttacag atgaaacatt gtccagtgga ggattggtaa
    16621 cacagggacc caacttgttt caatggagta aaggacctgc tacagattat atcgaacaga
    16681 cggtaaccaa aaataagcaa ctaattatag aagaaatcaa tcgtctggaa aaagatcagc
    16741 aatctactcg atgtttatac ggactctatc aacgctggtt tttctcaaag ttgcaagaag
    16801 aatttccagc ttctattcag tttgtttatt ctttagtaaa aaaagtaagc aaaagaaaag
    16861 atggttatct tttacagaca gatcagcaaa gctattctat cgatcagctt gttttagcaa
    16921 gcggtcactg ggaaaatgat gagacagaag aagaaagagt gtgggtagac tacgcaaaga
    16981 aaaatgtttt actgtatcag cttccttcta atcctgcaga tgtagatctt tcgaagatac
    17041 cgcctaaaga aaatgtcttt ttaagaggtc taggcttaag tttttttgat tatgtcggat
    17101 tgtttacagt atcaagagga gggcgcttcg agaacgaaaa gggaaaactg gtctatcatc
    17161 catcaggcaa ggaaccgatt gtatacagtg gatcaagaag ggggctccct tattaccctc
    17221 gaggaagaaa tcaaaagcaa ggcggagcaa tggcatggcc acggttacta acaaatgaaa
    17281 acctggagca atggcatcag tcgggatttt tgactggcga aacatttttt gactatctga
    17341 aaaaagacgt tgagttgttt tattacaaga aaatcatcga agaatatcac ttgccgattc
    17401 caaagaaaaa ttttgagcat gattttctct ccctctcttc agaggagtgt ctcggaaagc
    17461 atccagcact aatcccttat agatgggatt ggtcgtattt agaaactcct cttgcctatc
    17521 agaatgagcc cttttcagaa gctagccgaa tatttattga agggcagata aaagaagcgg
    17581 aaaaaggcaa ttgcacagga gcatttgctt caacatttga tgcattaaaa gactggcgtg
    17641 atccaatccg gcaggctatt gaatggcacg ttttttctgc taaggaatac aaagaaatac
    17701 tatggggatg gtttactcat ctaaatagct ttttaacgat cggtccgcca atcattcgga
    17761 cgaaagaatt agcagcactg atagatgcgg gtatttttca tttgatcgaa ccgccatttg
    17821 atattcagca aaagcacaac cgctatgtta tttctctaca agataaagaa atcagcagtc
    17881 gctatttgat tgaagctcga ttgccacaaa caagcttttc tcgttcaagt aatcctgtca
    17941 taaaaaattt gaaggaaaac aaacttgttc gccctttttc attttcagat aatacaggaa
    18001 gttatcattc agaagctata gatgttgagc gaggtacgaa tcgcgtgatt gatgcttcag
    18061 gggaaatcca gtctgacttt tattgctatg gaatacctgt tgaaggtgtg gattggctaa
    18121 ctgcagcagt agctagacct tatacagatc catggaatct gagacaggca gataaaatag
    18181 cacaacttat attgactgac aaataaaaat acgcatgatg acttatctgt tttttagaaa
    18241 gtcttcatgc gtatttttaa ataattatta acgagaaacg cgtttacgca ttgctttttt
    18301 acggttttct tcgatcgttg cttctttttc gtcaattgga tcgatcactg tttttttcac
    18361 agaagcaacc aaaccagcaa cggcagcggc tgttgccaga cttcctacta atattccaga
    18421 tacaaatttt ttcattgaat ccacctcatt tattatttgt tatatctatt atggaacaaa
    18481 agagaaaaaa aggctagttt tacgtttgtt tgtaaacctt tgcaaaaaat ccttgaaaat
    18541 gtttactaaa tgttttatta taaatttacc atatgaattt acgaaatttt actttgtttg
    18601 ttttcaccat aaaagtaaac ttcgtttttt aaattatata gaatcaacaa acacaatttt
    18661 actaatcaaa ggagtgtttt ttatgacaat tttagtacta ggcggagccg gttatatcgg
    18721 ttcgcacgct gtagaccagc tagttcaaaa ggggtatcaa gtagctgtgg tagataattt
    18781 gctcacaggt cacaaacaag cagtacatcc agatgctcat ttttatgaag gagacatccg
    18841 ggataaagaa tttctacgtt ccgtatttga aaaggaaccg attgaaggag taatccattt
    18901 tgctgcaagc tctttagtag gagaatctgt cgaaaaacca ttgatgtatt tcaacaacaa
    18961 cgtatacggt atgcaaatct tacttgaagt catgcacgag ttcaacgtga ataaaatcgt
    19021 attctcttct actgcagcaa cttatggaga gccgaaagag tctccaatca cagaagatac
    19081 accagctaat cctaaaaatc cgtatggtga aagtaaattg atgatggaaa aaatgatgaa
    19141 gtggtgtgac caagcatatg gcatgcgcta tgtcgctttg cgctacttca atgtagccgg
    19201 agcaaaagca gatgcatcta tcggagaaga tcatacacca gaaacgcatc ttgtcccaat
    19261 cattttacaa gtagctcttg gtcaaagaaa agccttggct gtttatggag acgattatga
    19321 tacaccagat ggaacatgta tcagagatta tgttcaagta gaagatttga ttgctgcaca
    19381 tattttagct ttggaatact tgaaagaagg aaatgaaagc aacttcttca atttaggcag
    19441 cagcaaagga tactcagtca aggaaatgct agaagcagct agagaagtga caggaaaaga
    19501 aattccagca gaaatagcac caagaagagc tggagatcct agccgattag tcgcttcaag
    19561 tgaaaaagcg agagagattc tgggctggaa acctgaatac acagatatca aagcgattat
    19621 caaaacagca tgggattggc atgtcagcca tccaaatggt tatgaagaat aggggaatga
    19681 gatatgtcaa tcagtcaaac gatttgtgat tttgccacac ttgcgatcga agctggcgga
    19741 tggatggaaa tggaccgctt atatttacag aatcgtcttt tagggatggt aggagaagat
    19801 gctctagaaa gcattgaagt aagaccagtt aaggaagaat ctgtagactt attggatcag
    19861 cttgtcgcta aggcaagaga aaatggtgtt ctttcagaat tgccagcaga acaagagatt
    19921 tttgaagcgc aattaatgga ttttctcaca ccgccacctt cagtcgtcaa tgcattcttt
    19981 gcacagcact attcgaaaag cccagaggaa gcaactgagt atttctttaa tttaagtaag
    20041 aaaaatgact acatcaaaac aagagccatc gcaaaaaaca tcaaatttcc ggcagagtcg
    20101 gagtatggtg aactggaaat cacaatcaat ttatctaagc cagaaaaaga tccaaaacaa
    20161 atcgcagcag aacgcgaggc ggtaaaagta gactatccta cctgcatgct ttgcatggaa
    20221 aacgaaggat atagaggtcg cttgaactat ccagcccgca ccaatcaccg gattattcga
    20281 atgaatctag acggtgaaag ctggggattc caatattccc cttatgctta ttacaatgaa
    20341 cattcgatca ttttatcaga acaacaccgc ccgatgaaaa tcacaaagga aacatttgct
    20401 agactgcttc ggattactga agtcttgcct cattactttg ttggttcgaa tgctgactta
    20461 ccaattgttg gaggatcgat tctttctcat gatcattatc aggcaggacg tcatgaattt
    20521 cctatggcaa aagcaccgat agaaaaactt atcaaactag aagattatcc gcaagtcatc
    20581 gcaggagtcg tgaaatggcc gatgtctgtg atccgtttgc aatcaacaga gaaaaacttg
    20641 cttattgaag cagctaactc agtattggaa aaatggaaag catattcgga tgaaacagta
    20701 tctatcagag ctttttcagc agatggtaca ccacaccata cgattacgcc aattgcacga
    20761 agaagggaca attactttga gttggacctt gtccttcgag acaacaatgt ttcagaagaa
    20821 catcctgatg ggatttttca tccacataaa gatgtccaac atatcaaaaa ggagaacatt
    20881 gggttgatcg aagtaatggg gcttgctgtt ttaccacctc gtttgaaacc agaactagta
    20941 gaagtagagc atttcttatt aggagaagaa aaccagatgg ctgaatatca tcgtccgtgg
    21001 gcagaacaat taaaaaaaga acatcctgat ttgacgaaag atacggtcac cgatgtcgta
    21061 caaaaatctg taggtcaggt ttttgcacgt gtactggaag acgctggagt attcaagcga
    21121 gatgaaaaag gacaggcagc gtttgaacga tttactgcga ttttataaac gtaaaaaagg
    21181 aggagagaca aatggcaaca attaaagaca ttgcgcagtt ggcaggagtt tctcctgcta
    21241 ctgtttcaag agttctcaac tatgatcctg atctttccgt tggcttagcc acaaagcaaa
    21301 aaatttttga agcagcagaa cgcttaaatt atacgaaaca tcgtaaaaat gcagcaaata
    21361 ctaagcaaac gcttcgcctg atccagtggt acgacgatac ggaagagctt gaagatcttt
    21421 attatttatc tattcgccta ggaatcgaaa aaaaggcaga agaagcaaac gttactttat
    21481 tgaaagaaac gtgggattcc attagtgatc ttccggcaga tggaacgatc gcactaggaa
    21541 aattcgacga agctcagtta ttagaaatga aaggatccca aaatgccttg cttttggtag
    21601 attctgacgg cacttctcaa ggaatcgact ctctagttgt cgactttaag tcaagtgttc
    21661 aaaaagtcat tgaacacttt gtctcccata aagcgcaaca tatcgctatg cttgctggaa
    21721 cagaatatac gaagaaaaac catcagcggc taaaagatcc cagaattatg gcatttcaac
    21781 aagttttggc agaaaaaggc tacgacgaag gtccaattat cgaagcagat ttttctgtcg
    21841 agtcaggata tcaagcagtg aagcagtatt taaaaacgaa tccgcatgtt cctgatgcgc
    21901 tttttgcagc taatgatgca ttagcgattg gcgcactgaa agcgattcaa gaaaaaggtt
    21961 tagtggtacc agaagatatt tctgtgatgg gcttcaatga tgtcagtgta gcgaaatatg
    22021 taacgccctc gctttctact gtaaaagttt ataccgaatg gatgggagag ctagcagtgg
    22081 agacgattct tcaattaagt aattccaaag caccggtttc aagaaagatt attgtggaaa
    22141 cagagttgat tattcgagaa tcaacgaaat aaaagatgga aaagctgtgg gaaaatggag
    22201 tgataccgca gcttttttgt gtttcaggaa ggggcaaaat ttgcccgcca gcagataatc
    22261 atgtattatt gtactagttt tgcctttggt attttcatcc tcagaataaa aaagtatgct
    22321 acaatggaag gcgagaatgt catagcagaa aggatcaaat atatgattac attaggaata
    22381 gatactgcaa accaaacttt ggcgatcggt gtggtagaag acgaacaaat tcttggacaa
    22441 atacaaacaa atatcaaacg caaccacagt gtaacactga tgcctgcaat cgaccaattg
    22501 tttgcagatc ttcaaataag tccaaaagac attgaccgaa tcgcggtttc tgatggtccc
    22561 ggttcttata cagggcttcg tataggtgta acgacagcaa aaacgattgc ttatacatta
    22621 gataaagagc tagtaggagt atctagtctg aaaaccatcg cggctaattg catcggtgtg
    22681 gataatatta ttgtgcccat gtttgatgct cgaagaaaaa atgtatacgc tggggcatac
    22741 cgctggaaag aaaatagatt agaaactgta cttgaagatg tacatatttc tgcttctgat
    22801 ttgttcacac aactacaaaa gctgaatgaa gctatttatt tcgtcgggag tgattgtcac
    22861 aaattcttct ctgaaataaa ggaaatattg cctagtgcga tgattaatac agttccagca
    22921 tgggatattc caagtggtgt aactgtggct caattaggtg ctttagttga accagttgca
    22981 gagaaacaag cttttcttcc tcgctattta aaacgagtag aagcagaaga aaaatggttg
    23041 gagacgcaca cacctggaga cgaaagttat gttgaaaaaa tttagagcgt atgccagaag
    23101 cttttttcaa gataaaatga tttatacaga aaaaacagtc gaacaaatcg atcgtcgtta
    23161 cgtactaaga gaattgacta cagaagatat caaagagctg ctggatgtcg aacgagaagt
    23221 ctataatggc gaattgccat ggacaaaaag tgcctttata tcagaattga agtcaccgct
    23281 tcctcatcta tatctaggta tctttgataa tggaagaatg attggtttta tcggttcaag
    23341 agcgttcggt ttagattgcc atatcacgaa tattgccgtt cgaccggcat atcagcgtaa
    23401 acgaatcggt tcgctgctta ttgacgaaat cgagaacttt gctataatga atcgctgtga
    23461 gacgatgtca ttagaagtgc gtatgagtaa tcaagatgca caaagacttt atcggaaatt
    23521 ggggtttcaa gcaacaacga tcaaaaaaag atattacact gaaacaaaag aagatgcgtt
    23581 ggatatgata aaacttttag aaaatgagtg agattagttg atttgcagaa aaaaagattt
    23641 tgatgaagcg tacttggcag aatgcgtatg gatgattagc gaaaatgctt atccacatgg
    23701 ttcaccttgg acgaaaatgc agtttttggc agacatccaa cagcctcact ctgattatct
    23761 gcttttagct gaagataacc agatctgtgg ctttattgga tactcgaaag taatagatga
    23821 agtcgagatc acaaatattg ctgttgcagt ttcagaacaa ggaaaagggc atgcacggca
    23881 tttgttacaa ttactgatta cggaacaaat aaaagattcg ctgcatgttt ttttagaagt
    23941 acgtctatct aatgaagcag caagaaaact ttatgaatca gaaaagtttc gtatattagg
    24001 taaacggaaa tcatattatc gcaatccgac ggaagatgcg atcattatga gcagaaagtt
    24061 aaaaacggag acgatgaaat gaatgaagaa ttgattctgg cgattgaatc aagttgtgat
    24121 gaaacgagtg tagcggtcgt tagaaatgga acagagatct tatctaacat cgttgcttct
    24181 caaataaaca gtcataaacg ttttggtggc gtagtacctg aagtagctag ccgccatcac
    24241 gtagaacaaa tcaccttatg cttggaagat gcactggtag aagctggtgt gtcggcagaa
    24301 gatttatcgg cagtcgctgt cacttatgga ccaggacttg tcggctcgtt gctgattggt
    24361 atttctgcag ctaaggcttt tgcttgggca caccaattgc cgcttattcc tgtaaaccat
    24421 atggcagggc atatctatgc agcccgtctg gttaaaccat tccagtttcc attgatggca
    24481 cttcttgtta gcggaggaca tacagaatta gtctatatgc aagaagatgg atcatatgaa
    24541 atcattgggg agacaagaga tgacgcagca ggagaagctt atgacaaagt tggaagagta
    24601 ttaggtcttt cttacccaag cggaaaagaa atcgatcagc tagctcatca gggaaaagat
    24661 aattaccatt ttccgcgagc gatgatccat gaagataatt atgatttcag ttttagcgga
    24721 ttaaaaagtg catttatcaa tttggtgcat aatgcacaac agcgtggaga agatctagac
    24781 aaaaatgatt tagcagctag ctttcaagca agtgtgatcg atgtattgat tactaaaaca
    24841 cttcgagctt gtcaaaatta tccagtcaag caactgatta tagctggagg tgtagcggcc
    24901 aatcaagggt tgcgtgaagg attgcaggat gctttggcaa ctaaactccc tgaggtagaa
    24961 ctagtgattc cccctcttcg actttgtgga gataatgctg caatgattgg agcagcagca
    25021 catgtggaaa tgcaaaaaag aaattttgct agctatcagt tgaatgctga tccaagcttg
    25081 gttttatctg aataaagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc
    25141 caggaaaaac ggcttctcat gtttttgacc tggctgttct tattaccgcg tatgcaagct
    25201 tttgaacgcc gtttaatcaa gagttcgtaa gcctgtcgtt taactctgat tcacaaggaa
    25261 caatttcaaa aaacagctcc tcatgtttat gaacattatg acttgtgacc gaagagttga
    25321 caggtacaca ccgtttaatc gaaatagggc tgtgacacgt ttttgtcaaa gccctatttt
    25381 ttacatactt ttaatcatta ttgttatttt gctatttttt acagcttatt tatcattatc
    25441 cgttcctctg attgccctca ctgagtaatc gtttattgtc gaggaatatc gaataatcta
    25501 gatcggatga ggacaagacg cttcatctag ttaatctaac agtatttttt gcaaacagtg
    25561 aatattacat aactgaaaca cacatagaaa atctttgagt cttgtccagt gatctaaaac
    25621 tgccggatct ttcagaagtt ttagggagga aaataagcag tgaccctgat atttatcagg
    25681 gtcactgctt atttttgttc gcgttattct ctcatagctg gagtagtagc tgtcttacag
    25741 aaagtaaaac gctttttgtc ataatctaac agcccggaat cacgtagttg tttgattact
    25801 tgtccagcag tttcacgtgt cgtgccgcta ttcactgcta attcttttaa ggtgatcgga
    25861 taaggaatgg tgatatgacc atcttttgtt ggtacgccca attcttcttt caaaatgaat
    25921 aaattttgtt ttacccggtc aaaggcgcta gaggtcaatc cgatttggat acgcaattca
    25981 tgtatttcaa ctactttaga aattttttgt atcatataga tcaactgttc gggatttttt
    26041 cgtgctaatt tttcatagta ggaaacagga aggtagaatg tttcaatatc agtcatcgct
    26101 tgagctgtat aagtgtaggt ctcttctcca aacattcctg acataggaaa aaggttattt
    26161 gtattcacat agtcatagta gtaataagtt gcactttcat cataacgttc taaacgaacg
    26221 agtcctttgg tcaagatata caaccgttgt ctgggatcac catcgtcgaa aaggatctgt
    26281 ccttttttat aagaacgaag aacagtatgt tccttgatct gttggaactc ttcatccgag
    26341 aaatgttcaa aatcaggttg atatctgagt tttgaaaagt gaaaatcgat tttattaaca
    26401 cgcattaaaa agtcctctcc ttattgttca ctatatttat tgtcactttt tttaaagaat
    26461 ttgtaaagtt ttaacatacc aacattagaa acaagtttta atactttaca aatataattt
    26521 gttattatgt aaaaaatata caatggtatt gaataatttg ttttgaagcg aatgatttgt
    26581 gtataaaaaa caacaataat acatatagta aacgctaaca acgataatat aatttatttt
    26641 tttaatttat tcaaaaaaat tttttcaatg aataaatatt tttttgaaag tgtatacaga
    26701 ataaaaaatg aataaattac gcttaaaaat agaaaaacgt ttaattattt aattatcgaa
    26761 tattaatgtg aggaatgtct cattttgaat gagacaaaat tatgaaagtg attacaaaag
    26821 gaggatatcc tatacatgta atcaaaaaga aggaggcaac acaacatgga taaacctatt
    26881 cacgttttct ctgaaatagg gaaattaaag acagttttac tgaaacggcc tggacaagaa
    26941 gtggaaaatt tgacaccaga tattatggat cgtctgttgt tcgatgatat tccatatctt
    27001 ccgattgctc aagaagaaca tgacaacttt gcgaagacat tgcaaaatga aggcgtcgag
    27061 actctttact tggaaaaatt agcggcagaa gcgattgacg caggcaatgt caaagaacaa
    27121 ttcttgaata aaatgttgga tgaatctcat attgcatcga atgctgtaag agagggtctt
    27181 catgagttcc ttctatcaat ggaaacgcaa gaaatggtag ataaaatcat ggctggggtt
    27241 cgtacaaaag atatcgaagt acgttctagc agtctatatg atctgtcagc agacgatgac
    27301 tatccatttt acatggaccc aatgccaaac ttgtacttta cacgcgaccc ttccgcatca
    27361 atgggaaatg ggatgacagt caataagatg acttttgaag cacgtcgtcg tgaatcgatg
    27421 tttacagaat atattttgaa acatcatcca agatttgcaa ataaaggaat cgaagtatgg
    27481 ttggatcgtg aaaatccaga tcatatcgaa ggcggagacg aattgatctt aagcgataaa
    27541 gtagtagctg ttgggatttc tcaacgaact aatgcgaaag ctttagaaaa attagcacgc
    27601 cacctattcg caaaaaattc aggatttgaa aaagtgttgg caatcaaaat tccgaacaac
    27661 cgtgcaatga tgcatttgga taccgtattt acaatggtag atcacgataa attcactatc
    27721 catccagcaa ttcaaagtaa agacggtaag atggatgtct ttacaatcga accagatggt
    27781 gatgatatca agattactca ttcagataac ttacacgaaa cgatgaaagc tgctcttgga
    27841 ttagatgatc ttgtactgat cccaactggt aatggagatg agatcgttgc acctcgagaa
    27901 caatggaatg atggatcaaa cacattggca atcgctccag gtgttgtagt cacttataac
    27961 cgaaattatg tatcgaacga attgcttcgt agctatggca tcaaagtgtt agaaatcaat
    28021 tcaagtgaat tgtcacgagg tcgtggtggt ccacgctgca tgagccaacc acttgtccgt
    28081 gaagacttga agtaatttgt ttcctgacgg cagtcttact gtcagctgcc gtcgggattt
    28141 cctctttaat cttaaataaa aacaatctaa aggagaatgt ttaatatgaa agaatcagta
    28201 ttccaaggaa gaagcttatt agcagaaaaa gactttacaa aagaagaatt acaatatttg
    28261 atcgattttt cagaacactt gaaagacttg aaaaaacgtg gtatcccaca tcactatcta
    28321 gaaggaaaaa atattgcgct tttatttgaa aaaacatcta cacgtactcg ttctgcattt
    28381 actactgcag cgatcgatct aggtgcacat cctgaatatc taggtgcaaa tgatatccaa
    28441 ttaggtaaaa aagaatccac agaagataca gctaaagtac taggacgtat atttgacgga
    28501 atcgaattcc gtggattcag tcaaaaaatg gtagaggaac tagcagaatt ctctggtgtt
    28561 ccagtttgga atggcttgac tgatgaatgg catccaacac aaatgatcgc tgacttttta
    28621 actatccaag aaaactttgg aacagtagaa ggtatcactg ttgcttactg cggagatggt
    28681 cgaaacaata tggctaactc tttattagtc acaggtgcaa ttttaggagc gaatatgcgt
    28741 atcgttgcac caaaagaatt acaacctgaa gaagaaatcg tcaaaatggc agaaggcttt
    28801 gctgaaaaat ccggtgcaca attgatgatt accgacgatg ttgataaagg tgtagacgga
    28861 gcagacgtcc tttattctga tgtatgggta tcaatgggtg aagaagataa attcgaagaa
    28921 cgtatcaaat tgttgaaacc ttaccaaatc aatatggaaa tggtagaaaa aacacacaac
    28981 acagatcgtt tgatcttctt gcactgcttg ccagctttcc atgatacaaa cactgtttac
    29041 ggtgaacaaa tgaaagaacg ctttggcatc actgaaatgg aagtaacaga tgaagtcttc
    29101 cgcagcaaat atgctcgtca atttgaccaa gcagaaaatc gtatgcactc aatcaaagcg
    29161 atcatggcag caacattagg taacttgttt atccctcgcg tttaagcata accacattca
    29221 tctcacgaaa aaacgagtca gcattgaggg aaaatcacaa aacgctggct cgttttgtta
    29281 tctagaaaat tcggtagaac tgaagaaagg acgaatgaaa tggccaaacg aaaagtagtt
    29341 gtcgctttag ggggcaatgc catcttgtct acggatgcaa gtgcaaaagc ccaacaagaa
    29401 gcgttaatgg aaacagcaaa gtatttagtg aaatttatcg aacaaggaga tgaattgatc
    29461 atctcccacg gaaatggtcc gcaagtcggt aacctattga ttcagcagca agctgctgat
    29521 tctgagaaaa caccggcaat gcctttagat acatgtgttg caatgacaga aggatctatc
    29581 ggttactggt tacaaaacgc catgggcgaa gtactgaagg aaaaagggat cgataaagat
    29641 gtggtttctt tagtcactca agttattgtg gatgaaaatg atccttcatt caaaaatcca
    29701 agtaaaccag tcggtccatt ctacacggaa gaagaagcca aagaacaaat gaatgctgac
    29761 tccactgtga cattcaaaga agatgctgga cgtggatgga gaaaagtagt cgcttcccct
    29821 aaacccatct cgattaaaga agcacgtgtc atcgaaacgc tggtcgatca aggagttatc
    29881 acagtctctg ttggtggcgg tgggattcct gttgtagaaa cagctactgg attagaagga
    29941 cgagaagccg taatcgataa agattttgct tcagaaaaat tagccgaaat catcgatgcg
    30001 gatttgctga tcgtattgac aggagtagac aatgtgtatg tcaattacca aaaaccagat
    30061 caaaagaaat tagaaactgt tactgtttca gaaatgaaac aatacattga tgaaaagcaa
    30121 ttcgctccag gaagcatgct tccgaaagta gaagcagcta ttgcatttgt tgaagcaaaa
    30181 ccaaacgcta aggcaatcat cacttcttta gaaaatatcg agaatctttt agcttctgaa
    30241 gaaggaacga ttatcgtagc tgattaattt gttaagtctt tcgtatttac gaaatgattt
    30301 atagctatcc aggtggtatt tcttctcctt taggccatct ggattttt
//
LOCUS       DASHRQ010000031        29110 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.031, whole genome shotgun sequence.
ACCESSION   DASHRQ010000031 DASHRQ010000000
VERSION     DASHRQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.031"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1030)
                     /locus_tag="U2M88_001507"
     CDS             complement(<1..1030)
                     /locus_tag="U2M88_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM8373894.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="U2M88_001508"
     CDS             complement(1112..1801)
                     /locus_tag="U2M88_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEM8373895.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     LKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="U2M88_001509"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="U2M88_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEM8373896.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="U2M88_001510"
     CDS             complement(2328..2831)
                     /locus_tag="U2M88_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEM8373897.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3035..3162)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="U2M88_001511"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="U2M88_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEM8373898.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="U2M88_001512"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="U2M88_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEM8373899.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="U2M88_001513"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="U2M88_001513"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEM8373900.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="U2M88_001514"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="U2M88_001514"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEM8373901.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="U2M88_001515"
     CDS             complement(6443..7462)
                     /locus_tag="U2M88_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEM8373902.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8540)
                     /locus_tag="U2M88_001516"
     CDS             complement(7635..8540)
                     /locus_tag="U2M88_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEM8373903.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="U2M88_001517"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="U2M88_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEM8373904.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="U2M88_001518"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="U2M88_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEM8373905.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="U2M88_001519"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="U2M88_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEM8373906.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="U2M88_001520"
     CDS             9753..10397
                     /locus_tag="U2M88_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEM8373907.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="U2M88_001521"
     CDS             complement(10453..10956)
                     /locus_tag="U2M88_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEM8373908.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="U2M88_001522"
     CDS             11553..12518
                     /locus_tag="U2M88_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEM8373909.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="U2M88_001523"
     CDS             complement(12617..13789)
                     /locus_tag="U2M88_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8373910.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="U2M88_001524"
     CDS             complement(13776..14921)
                     /locus_tag="U2M88_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8373911.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="U2M88_001525"
     CDS             complement(14935..15867)
                     /locus_tag="U2M88_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8373912.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="U2M88_001526"
     CDS             15984..17063
                     /locus_tag="U2M88_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEM8373913.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="U2M88_001527"
     CDS             17060..17689
                     /locus_tag="U2M88_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8373914.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="U2M88_001528"
     CDS             complement(17799..18026)
                     /locus_tag="U2M88_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEM8373915.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="U2M88_001529"
     CDS             complement(18129..20717)
                     /locus_tag="U2M88_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEM8373916.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="U2M88_001530"
     CDS             complement(20737..21396)
                     /locus_tag="U2M88_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEM8373917.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="U2M88_001531"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="U2M88_001531"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEM8373918.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="U2M88_001532"
     CDS             complement(22169..23035)
                     /locus_tag="U2M88_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEM8373919.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="U2M88_001533"
     CDS             23144..23785
                     /locus_tag="U2M88_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEM8373920.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="U2M88_001534"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="U2M88_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEM8373921.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="U2M88_001535"
     CDS             complement(25226..26125)
                     /locus_tag="U2M88_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEM8373922.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="U2M88_001536"
     CDS             complement(26127..26924)
                     /locus_tag="U2M88_001536"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEM8373923.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="U2M88_001537"
     CDS             complement(26902..27606)
                     /locus_tag="U2M88_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEM8373924.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="U2M88_001538"
     CDS             27737..28312
                     /locus_tag="U2M88_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEM8373925.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..29107
                     /locus_tag="U2M88_001539"
     CDS             28430..29107
                     /locus_tag="U2M88_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEM8373926.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8566 a         6168 c         5232 g         9144 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgagatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gcttatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taattaaaaa
//
LOCUS       DASHRQ010000032        27634 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.032, whole genome shotgun sequence.
ACCESSION   DASHRQ010000032 DASHRQ010000000
VERSION     DASHRQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.032"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(23..388)
                     /locus_tag="U2M88_001540"
     CDS             complement(23..388)
                     /locus_tag="U2M88_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373927.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYAVCIIKRDK"
     gene            complement(563..2698)
                     /locus_tag="U2M88_001541"
     CDS             complement(563..2698)
                     /locus_tag="U2M88_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM8373928.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2781..4436)
                     /locus_tag="U2M88_001542"
     CDS             complement(2781..4436)
                     /locus_tag="U2M88_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEM8373929.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(4438..4752)
                     /locus_tag="U2M88_001543"
     CDS             complement(4438..4752)
                     /locus_tag="U2M88_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEM8373930.1"
                     /translation="MNLLFQSIKSITVPELKEKILENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4922..5182
                     /locus_tag="U2M88_001544"
     CDS             4922..5182
                     /locus_tag="U2M88_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEM8373931.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            5196..5510
                     /locus_tag="U2M88_001545"
     CDS             5196..5510
                     /locus_tag="U2M88_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEM8373932.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(5995..6549)
                     /locus_tag="U2M88_001546"
     CDS             complement(5995..6549)
                     /locus_tag="U2M88_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEM8373933.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6675..8603)
                     /locus_tag="U2M88_001547"
     CDS             complement(6675..8603)
                     /locus_tag="U2M88_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEM8373934.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(8909..9625)
                     /locus_tag="U2M88_001548"
     CDS             complement(8909..9625)
                     /locus_tag="U2M88_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEM8373935.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9626..11449)
                     /gene="glpO"
                     /locus_tag="U2M88_001549"
     CDS             complement(9626..11449)
                     /gene="glpO"
                     /locus_tag="U2M88_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEM8373936.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11463..12959)
                     /gene="glpK"
                     /locus_tag="U2M88_001550"
     CDS             complement(11463..12959)
                     /gene="glpK"
                     /locus_tag="U2M88_001550"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEM8373937.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(13095..14555)
                     /locus_tag="U2M88_001551"
     CDS             complement(13095..14555)
                     /locus_tag="U2M88_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8373938.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14545..15840)
                     /locus_tag="U2M88_001552"
     CDS             complement(14545..15840)
                     /locus_tag="U2M88_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEM8373939.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(16172..16849)
                     /locus_tag="U2M88_001553"
     CDS             complement(16172..16849)
                     /locus_tag="U2M88_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEM8373940.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(16879..17970)
                     /locus_tag="U2M88_001554"
     CDS             complement(16879..17970)
                     /locus_tag="U2M88_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEM8373941.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(18071..18505)
                     /locus_tag="U2M88_001555"
     CDS             complement(18071..18505)
                     /locus_tag="U2M88_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM8373942.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(19089..20546)
                     /locus_tag="U2M88_001556"
     CDS             complement(19089..20546)
                     /locus_tag="U2M88_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEM8373943.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENL
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20543..22438)
                     /locus_tag="U2M88_001557"
     CDS             complement(20543..22438)
                     /locus_tag="U2M88_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM8373944.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22496..24271)
                     /locus_tag="U2M88_001558"
     CDS             complement(22496..24271)
                     /locus_tag="U2M88_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEM8373945.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYLVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESSEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(24321..25817)
                     /locus_tag="U2M88_001559"
     CDS             complement(24321..25817)
                     /locus_tag="U2M88_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEM8373946.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     EIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRIWKEEDCAPVMLDGDEQYLQANTVDYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(26011..27633)
                     /locus_tag="U2M88_001560"
     CDS             complement(26011..27633)
                     /locus_tag="U2M88_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEM8373947.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
BASE COUNT         7828 a         5951 c         4601 g         9254 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgtattcaa
       61 aagatttatt tacttttttg tatagatttc gattgttcca cctagtcaat acaaatgagg
      121 taatcaggaa actgaaaaaa atcaatacaa aaaagccgat taagaaatac agcattttct
      181 tttctgataa aaaagcgtac aaggaaaaat ccttggctct ttctggtctc gttaatggga
      241 aaaagaccca tataaaagga agaacaacga tcatccatcc taaaagagcc actaacgttt
      301 gccaaatctt taaccagtaa ttaccttttg aaaaatactt atctttcacc aaaattgatg
      361 caacttcttc tcttgttact tttttcatat cctcacctca cgtttttttg ttacgacgta
      421 ggagtttacc atacatttca tttcttttca cataaagtgt tcctaaattt ttcctaagaa
      481 aaagaacaaa acatttctca tcagcaaatg aaaaatattt tgttcttttt atcagttgtt
      541 ctttttattt tttatctctt aattatgttg ctctttccaa tacgacaatg cttttcattc
      601 ctctagatat cgccgtgtac atttgtctgc gtcttctttg ctcatctcca tcgttttcaa
      661 atggatataa gattacatga tcaaattcca gtccttttgc tagactcaaa ggaatgattt
      721 ggcagttttc taatccatat gaattttttt taatcgattc ttgtagcgtt tgggtttcct
      781 cttctgtttg ggtgataact gccgcttttt ctccttttag agaaaccaaa atctcagcta
      841 gtattttcaa caattcttca gaggatcgaa aagagcggta ttcgggttct tttccttgcg
      901 gtcggatcga tacgatgtcg atcttctctt gatccacagc ataggttttg aaaagctctg
      961 tgattgctcc gctagaacgg taactatttc taagatcata ccgatggatc ggcagatggt
     1021 agtcatcgaa acaacgcata atatcagcaa aagtagtgga agaattaaag attgcctgat
     1081 tctcgtcacc tactaaagta aagtgagttt ttggaaataa ttcgatcaat agacctaatt
     1141 gtgccagggt ataatcttgt acttcatcaa tcaatagata gcgaaatgct ggtacattta
     1201 ccttttcaac aagtaaatga cgaatagcta atataatgac tgcttcatca acagtacgtg
     1261 tgggttgcca agcataagct gagccatatc gtttttcata aattctttca aacaactgat
     1321 gctcatccac ccatgccatt tcttcgattt gtctggttat ttttcgatat tttttttgta
     1381 aaagtttcct tgcataagct acaatacttt gttccgaatc atcagaaatc aatttaccga
     1441 ataattccaa ctgcctatct tctgtcaacg agagtacctg attctgcaca gcaggacttt
     1501 ttgcttgttt caaaagatga ttcttccatt cgctcatcag tgcctttttc gttgcttgaa
     1561 gcctatcgat caatttagga tgagcaggaa ctttctcata tattttttcg atcgtttttt
     1621 tagaaaggac tgtttttcca tttttagtaa gtccacggat aaaattcgga tgatctaaaa
     1681 acaaaggatc tgattgtttt agattatcga taaacttttt gctacgcaaa cgttctgtct
     1741 gctccgatac attttctccc gtgatacgtt tgaaataagc ctcttcacct tctatttcct
     1801 ctgctgaaag ctgtgaaacc agctgtatga tggttagatt cagtggattt ctttcaccta
     1861 atgatggtag gacattcgaa atatagtcga taaaccgatg attaggtgat agaatcagca
     1921 agtcatcgga tgtcatcgta tctcgatata aatagagcag atacgcaata cgctgcatga
     1981 tagcagaggt tttaccactt cctgctacgc catttaccaa gatattttga cttttcacat
     2041 cccgaatgat tgtgttttgt tccgattgga tcgtagcagt gatagccttc atttcattgg
     2101 tctcatcttg gcctaagact tctaataata cgtcatcttg aatagctaca gacgtatcaa
     2161 aaaacttgac caaacgatct ctttccaaaa gaaattgtcg tctttcttta ataaccgtat
     2221 cgattttatt ttgacgaacc gtataagaag atggaccaat catgttattg taaaaaagtt
     2281 cggcaattgg tgaacgccaa tcataaacaa gattttcatc tgcatcatct gtgaaatttg
     2341 ctgtaccgat ataaaaggat tcttttggct cattatccag aaagtctaca acgacttttc
     2401 caaagtaggg acttttcaat aaacgttcga tctttgctag ctgattttct gcgtttttct
     2461 gtttgatatt catctgatcg atttctctat ttttcatttc aatcatcgaa aaagtatcca
     2521 ggttatctaa gtaactgtca aaattcaatc gaagctcgcc agccatttct ttcaacgact
     2581 ggtttccttc tatttttgcc gtatccatcg attcccgcag ttcgtttttt ttatcaatta
     2641 gcttttgata cacgttcttc agatatgctt cttctaactg tttttgtgtg ttgaccatac
     2701 acattctcct ctttttttaa agtggtggaa ccgattagtt tacctctttt aagagtcgaa
     2761 tgcaagcttt ttaacgagta tcatttgtta ggatatatca gttcttcagg tcggacagtt
     2821 tgatataaag aaaatgcacc atcaaggttt tttacgtggt agccagactg cttcaagatt
     2881 ctttccgcta gatagctacg taatccgcta tgacagctga cgatatattc ttgttggctg
     2941 tctaattcat ttaatcggtc ccgaagttca ttcaaaggga tatgtttggc ttccttgaat
     3001 ttgccctttt ccaattcttc tgctgttcgc acatctaata aaatttttcc ttttgctagt
     3061 tctgtcggca attcatgcca ttgtacggtt tcactaagcc cttctgcaat gttcattgct
     3121 gcatatccta gcatgtttac tggatctttg gctgagccaa atggcggtgc atacgtcaat
     3181 tctaattccg gtaaatcgaa gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg
     3241 atccgtttat ccacaccttt tgccccaaca ccttgtgcac cataaatctc gccagtctct
     3301 ggatggaaga tcaacttcag taaaatatct gttgcttctg gataatagct tgcatgatct
     3361 tttcctgata catgaatgac ttcaaaagac agttcgtttt ctttggctgt tctttcactt
     3421 aaaccggtgg atgcggcagt cattccaaac gttcgtataa ttgctgttcc aataccgcct
     3481 tgatttcttc gagaaattcc ggaaatcgtg tccgcaactt gtctaccttg acgattggct
     3541 ggtgaagcaa gagaaataag tgcatctttt ccagtgattt gctgttttac aacgatagca
     3601 tcccccaccg cataaatatc aggaatattc gtttgataac gttcgtctac taggataccc
     3661 ccgcgtatgc ctaattcgat tccagcggtc actgctagct ggttatcagg acgaacacca
     3721 atggataaaa taactaaatc agcagggatg gtacgcccat catccagtcg caatcgtttc
     3781 ccgtggtctt caaatccagc gacagcatga gaagtaatca cttctactcc ttgatgagac
     3841 aattcttttt ctataaaagc tgccatttct tcatccagtg ttggaagaat atgtggtgct
     3901 tgttcgatca ccatgacttc taaacctctt ctttttaggt tttctgccat ttccaatccg
     3961 atgaatcctg caccaacgat cacggcacgc tttggctgtt tttctaatgc atgcatcact
     4021 ttatcgatat ctggtacatt tctaatagaa aaaacattgt cggcttctgt tatccctgga
     4081 atcgatggaa caactggttt tgcaccagga gataaaacca atgcatcgta ctgttcaatg
     4141 tgtttttgac cctcatgtac gacttctacc gttttgtttt cgggaaagat ggctgtcact
     4201 tcatgatttg gacgcacatc taaacaaaaa cgagcggata atgattctgg cgtttggaca
     4261 agaagatttt cccgttcact gatttctccc gaaagataat aaggcaagcc gcaatttgca
     4321 aaagaaacat acggtccttt ttcaaaaaca acgatttctg catcttccat taatcggcga
     4381 agccgtgtag cagcagacat tccacctgct acacctccga caataacgat tttcatctta
     4441 tttccctcct actgttcgat caaaccattg attcatgcca ccacgtacat tcactacatc
     4501 ataaccagat ttctttaatt ctttagctgc ttgtttactc ctcatccctg attgacaaat
     4561 cacatagacg gtcttttctt tgtctccatc atatctgttt atactttgta atggaacatt
     4621 tttagctcct tttatatgcc cgccccggta ttctgcaggt gttctaacat caagtaacgc
     4681 tggattttcc aaaatctttt cctttaattc tgggactgtt atcgatttta ttgattgaaa
     4741 tagcaaattc attttctgca cctcatttac atttttataa atacctcatg gggtatatta
     4801 aacaggagct ttattaatat gtcaaccaga atgactcttc atttttttca ctacttttta
     4861 tatttgcata aaataccgat aaggggtatt atctaattat aaaaatagag gagaatgtgt
     4921 tatggagatt gataataaaa atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg
     4981 tggtatccaa aaaatgatcg aagacgaaaa agaatgtatg gatgtcatca ctcaattaag
     5041 cgctgttcgt tctagtattg atcgcgtaat gggcatgatc gttgcagata atctgaaaaa
     5101 ttgctttgag aaacctgata gtaatccaga agaacaagcg gctaaactag aacaagcgat
     5161 caagatgatt attaaaaagt aaaggaatga tttcaatgaa agtcgttttc catattgatg
     5221 aattagaaaa atggtcagaa acagggaaaa acgtaaaaaa cctgataaaa gcttctccag
     5281 aaacgacgat tgtcgtatct gtaaatggca tcgcgattac cggttatcta gactctgcca
     5341 atgcagaatt cttagatttg caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg
     5401 ctaatcatat ttcagaaagt tctttacctg aacaagtaat cgttgtacca gcaggtgtcc
     5461 ttgatttagt tgaactgcaa tcccaaggtt atgcttatat caagccttag tcagtagagg
     5521 ttgcgacaga agtgatcagc ttcaagaaat ttcggcttat ttttcgaaga agctacttct
     5581 ggaaaaccgt gaatcaaagg ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc
     5641 aaacaaaaat agcttctcat atttttcgtt tggttgggct tattaccgca gatgcaagct
     5701 tttgaacacc gtttaaggaa gaggttgtga aatcagcggt ttgacctaag tattagagaa
     5761 aaaaacggga aaatagctcc tcatattttg ccgttttttg agctaatacc gaaggtcagc
     5821 tgattgaaca ccgtttacca agaggttgtg agcctaccat ttaactccga atcacaagaa
     5881 ataatttcaa aaacaacttc tcctgttttt ggtttggttg gcttattatc gagaagtagc
     5941 tactgggagt ctatttatca aaaaaaggaa tgcgacatag gtcacacccc ttttttattt
     6001 tctttgaaac atctttcggt actcgacagc atacaagaca aaagattgta aaagaccgta
     6061 agtgatgacg gcaaaaatga ctctcaccca cagcgttggt aattctggga aaagcagcaa
     6121 aaggatcatg ccaacataga tgacagcagt cgctactttg ccataccact tcgctccact
     6181 taatttgata tttttagata gcccgattaa tccagctaca aacataaagc cttcttttat
     6241 caagaataga ccaaacaaca gccaaagcca cggtcgatac catgccatag acaaaatcag
     6301 aaaaagttgt gtcaatttat ctgcaaacgg atcaagcacc ttccctaagt ctgttacctg
     6361 attatatctc cgagcaatga ccccgtctag ataatcagac aaacctgaga caacaatgac
     6421 cgctcctgct atataaaatg actgtctcag cacaaaataa agataaatcg gcagcaaaaa
     6481 aatacggaat atcgataaaa gattcggaat cgtttgtatt tctttacgcc agtctgtgct
     6541 taatttcatt ttatcgccct acctcctcag cactcttttc tttttatttt agcttacacg
     6601 gggaataaaa ccaatgattt cactgttttt atttttcttt acagccactg atattatgag
     6661 ctaatttgag ataatcactt gctgttttca tcgtttactc ttctttcttc ctgtcgtttg
     6721 cgcatttctt ttactagtac tttatgagct ttttttgatc ctcgctctag gtcacgctgc
     6781 cgtttacgtg ttttataatc gataaacaga ctgtctaaat cgtaacctgt cgggtacaga
     6841 tcttctgctg aacgcaccag acgaacacgc tgacgaggca cgtggattat ttttttgtct
     6901 aaatatacat caatcgtatc ttcacctata tcttcataaa ctaacgcttc tttttggtat
     6961 tcattgacaa atatccgatc accttttgca aagagctggc gttctttttt attctccttc
     7021 ctatttgtaa ctgctgtctt aggaagaaat tcttttttct gtgtccgata ggctttttcc
     7081 gccaagtaac gctctgcttg atggatcaat ttcatcgaca tatccatttt atgagcaatc
     7141 cacaaagctt ggctttctcc tgtttctcca acccgcaact gatatttagg acgaagtgct
     7201 tcccgatcga aagccatagc agccggcaca aaatcttcat gatctccggc gaatttcttg
     7261 atttctccat aatgggtcgt ggcaatgatc aatgcccctt gctcatacat agattccatg
     7321 attgcgatcg ctagtgcagc cccttcattt ggctctgttc cactgccaat ctcatctaaa
     7381 agtaccaacg tattcctttt tatctttttc aaaatagcag cgatgttttg catatgacca
     7441 gaaaatgtac tcaacgcatt ttctaaattt tggtggtcac cgacatcgac gaaaatctca
     7501 tcaaagacag caagctctgt cccttctttt gccggaactt gcataccgaa catcgccatc
     7561 aaagtcaata aaccgactgt cttcaacacg actgttttgc ctccggcatt tgctccggtg
     7621 ataatcaagc ctcgatagtc tttaccgatt tcaacatcca atggtaccgc atgctcaggt
     7681 aaaaatggat gtctgccttg cttgataatg atatgttccg atttattgat tctaggtgtg
     7741 cttccattga tttctcggct aaattttgcc cgagcaaaga tgatatctaa caccgtgatc
     7801 gtttcgatca ataaatcgat cacttcttcg ttttctgcga tagcaccagt caatgccgcc
     7861 aggacttgat attcttcact gatttcttca gcttttaaca actgatagtg ttcattcaat
     7921 ttagaaatca cagttggttc aataaaaacc gtcgttcttt tatttgattc atcgataatc
     7981 gttccatcta ttttattttt atagctggct ttgataggaa tcgtatagta ttcgcctttt
     8041 tgaacaatca ttgcttcttg gatcatttct ttgtttttcg gatgacgcaa gaatttcagt
     8101 aattttgact ggatttcttt ttcgattagt tgtaattgtt tgcgtacttt acgcaaatta
     8161 cgtgacgcgt cgtcactgac tttattattt tttattttct ggtcaatcaa ctcttctaca
     8221 tttatcaaat caggaagatg tttgctataa gcaaacaaca agggtgtctg gtattgattc
     8281 ttatcaaaaa actttgtgat catccgactg cttctaagaa aatccgcata ttcgatcaaa
     8341 tcggttggct gcagaatcaa cccttttttg acttgttctg tcagtgcatc gatcctcggc
     8401 aaccccataa atggaacatg ctgactgctt tctaagatca agcgtgcttc tcttgtttct
     8461 tcttgacgat ccaagacagt ttgtaggttt gtcgagaccg tcatatcgct gattcgtttt
     8521 ttgctatagt ttccaattgc tcttgcttgg atttcctttt tgatccgatc aaattgtaat
     8581 tgactaatca tttcgttgtc catattctct ccttttcttg aagagatacc tgtcccaaaa
     8641 taaataaggg cctagagata aataaaagga tagaacacag cttaaaaaag tcatgtccta
     8701 tccttattag aatacaaaga tattgttttc ctgacaggta ccgtttcata agcacaccaa
     8761 ataaactttg tttattggct attcgactta ctgattagat accagctaca ctcacaaaac
     8821 ataccccatt cttctaggtc ttatttattt tccacttaac tatagcaaga cacattttgg
     8881 ttgtcaaact ttagtatgtt ttatagattt acaccattaa tgaatagaca cctaccgcga
     8941 taattgctcc gatcattgga cctgcgattg gtacccatga atatgcccaa tctgaagttc
     9001 ctttattagc tattggcaat accgcatgtg ctaatcgagg accaaagtcc cgagcagggt
     9061 tgattgcata tcctgttggc cctcctaatg aaagaccgat ggctaagatc aatccaccta
     9121 cagcgaatac gttcgttcca ggtgcaaatt cgttttgtcc aaaagctagc aacccaagaa
     9181 ctaatacaaa tgtaccaatt aattctgtga tcacatttgc tggataattc cgtatagcag
     9241 gacctgttgc aaacgttcct aaaatcgcgc tttcatcttt cgttgcattc caatgtggca
     9301 aataagaaag ccaaacaaga atggcccctg caaaagctcc taaaacttgc gcaatgatga
     9361 aaggaacgac aagattccaa ctaattgccc ctgtcatcgc catagctaga gagactgcag
     9421 gattcaaatg agctgggccc atgaatccag aaatatagac agccattgtt acagcgagtc
     9481 cccaaccaaa tgcgatgaca atccagccag aagcctgtgc tttacttttg tttaaattca
     9541 cagccgcaca cacaccatct ccaagcaaaa ctaggatcag tgtcccaata aactccccca
     9601 tgatttgagt catatcactg ttcatttatt tctccccctt tagttcccgc agatctgatt
     9661 ctgcaatcaa tgcttctaaa cgtttagtct gttgcgcctt ctcctcttct gtccattcaa
     9721 aatatccagc aattgcatcg atcacaggtt gcttgatctc atcaagctga tctcgttcaa
     9781 acaacagatg atttgtacga cgaatgagat aatcgcctgg ggccaaaacc atttcttctt
     9841 ctaaaccata acgtaaccga gctgactcag cgagactcaa accaggataa ggtgccattt
     9901 cttttgctaa ttcaaagata cgtcgggcat tagtcccgta aaaatcagcg atataagtag
     9961 catcttcttc ctctaaaccg gctgctactc cttcttttgc taattctgtc actgtttctt
    10021 ctaatttagt tggatcgaag tttccacctg aaatctgata ttttttagag tcgatttctt
    10081 tcgtctctat tccgtattct tcttttaata attggcgtat caatcgcaaa gctccttctg
    10141 ccatcttacg gtaatcggtg attttcccac cagataaagt cagcaaacca tctggttctc
    10201 tttctaaaga actacctctg gaaatcgtag aaggtgcttt ttcatcacgg ctgttttcca
    10261 aatggttcaa cacgtcttct acttcagcac gagaaacttt gttttcctta tattcactta
    10321 ctgtatcgac aactttattg aagctcttgt ctgaaatcga tccattatct ccaccattgt
    10381 aatcagaacc agaattacca atcaacagtg gacgaagtcc tgcccagctt gcttcgatat
    10441 cattcaatgt gatatttgct tctggatagc gatgattgat cacatccaat agataatcta
    10501 catcttcttg tgtgactttc ggatccgtaa aatctccttg gtaatccgta tctgtcgtac
    10561 caaagtaagt cttgttttct cttggaatag caaaaaccat ccgcccatct tgctttcctg
    10621 tatcgaagta tgtgggttgc ggtacaggca gttttttcgc atctacaact aaatggatcc
    10681 ctttagttgg acgcatttta ggagagactg gacgcgtaaa attcaagttt cgtactttat
    10741 ctacccaagg accactcgta ttgatcacta attttgcttt gatttcgatc acttcatctg
    10801 tcagcaggtc acgagctttg acgccaacga tttgatcgcc atcatataaa aagccaaccg
    10861 ctttcatttt acttactaga taagccccat cttcggcagc ctttttgata ttatcaatca
    10921 ctaaacgggc atcattgttg cggaaatcta gatagacacc cgcgcctttt agcccttctt
    10981 ttttcaaaaa cggttctctt tccaatactt cttctggtgt cagcgtataa ttctcatatt
    11041 tggttcctgt cacatttgcc agtttgtcat aaaggtccat tgctactttg accgagaaca
    11101 tattgaaggt tgttgctcct tcgccctcat agattggcaa aagcattgga tctggttttg
    11161 ggatgtgtgg ggcaatactt tgtacgactg cacgttcacc aactgtgtca gctactactt
    11221 ccacatcaaa tgtcttcaga taacgaatac cgccatgaac aagtttggtc gagcgagagg
    11281 aagtcccttc tgcaaaatcc tgcatttcga tcaatcctgt tttgattcct gatgctgctg
    11341 actggactgc cacaccggca ccagtgattc cgccaccgat gatcaacaga tccaattctt
    11401 cttgctgcat tttttgaatg ctatcttgtc ttgtcttgtt tgaaaacata tggatttcct
    11461 ccttattcaa atgcttgggt agcttttact gcttttttcc aaccgttgta aagtttttct
    11521 cggcgctctt cctccatttg aacttcaaat agcttgccag cttcataaaa ttcgcggatc
    11581 tcgtctgtat ctttccaata accgacagct aatcctgcta aaaatgctgc ccctagcgcc
    11641 gttgtttcca agttttctgc tcgttggatc ggtacattca agatatctgt ttggaattgc
    11701 atcagatatt cattgtttgc tgcaccgcca tctactttca atacaggaat cttgattcca
    11761 gtgtcatctt gcatcgtatc gatgatatct ctcacttgat aagcaatcga ctgtaaagta
    11821 gctttaatga tatcttcttt tgtcgtacca cgtgtcaagc caaacatagc ccctcgtgct
    11881 gcctgatccc aataaggagc acctagccca acgaatgcag gaacaacata aacctcatct
    11941 tcgcttgtag agcgtttcgc tgcgtcttct gaatcgctag ctttttgcaa catttgaagt
    12001 ccatctctca gccattgaat cgaagaacca gccacaaaaa tacttccttc taaagcatag
    12061 taaacctttc catttatgcc gtaaccaatt gtcgtcaata aattattttt ggataattgc
    12121 ggtttttcac cggtattcat gacaataaat gaacctgtac cataagtatt cttgaccatg
    12181 cctggttcga atgccatttg tccgaataat gcggcttgct ggtctcctgc catccctgaa
    12241 attggtgctt cgcttccata aaaatgataa ccttgcgtat aaccataaac ttctgagtta
    12301 gaagtgactt ttggtaacat cactcgagga atatttagta aatctaaaat ctcctgatcc
    12361 caatctagct gatggatatt gaacaacatc gtccgactgg cattcgagta atcagtaaca
    12421 tgtgcttgtc catcggtcag tttccatact aaccaagaat cgatcgttcc aaacatcaat
    12481 tcgccttttt cagctcgttc ttgcgcgcct tctacatgat caagaatcca gcgtatcttc
    12541 gttgctgaga aataggcatc gatcactaac cctgtttttt cgtggaaaaa ttcttgatag
    12601 ccttcatttt ttaagttatc tgcaattcca gctgactgtc tggattgcca aacaactgca
    12661 tgatagattg gacgtcctgt atctttttcc catattactg ttgtttcacg ctggttcgtt
    12721 atgccaatcc cagcaatctg atttggtttg atccctgatt cgatgaacgc tccggcaata
    12781 actgactgta cagaattcca gatttcattt gcatcatgtt ccacccaact ttcttgcggg
    12841 aaatattgcg tgaactcttt ttgagaactg ccgatgtgtc ttcctttctt gtcaaagata
    12901 attgctcgtg agctagttgt accttggtca atcgccatga tatacgttga ttctgtcatt
    12961 gtgattccct ccattatatg caaacgcttt ctttaactac tcttatcata ctactgtttt
    13021 aaaaaaagaa actgtcaaaa ttttattctt tttttctttt tttgacagtt ttttattatg
    13081 aagctatttt ttaatcaatt cttttctttt ttcagcgtct ctattcttct tttcaatcga
    13141 ataatgtcgt aaggtgacgc aaattctgac agataatatt cacactgttt tttgtttctc
    13201 tggcaaaatg tgatcaccaa atcatattct ttaccttctt cataacttcc aatctcaata
    13261 ccgccgattc ccctcagttc tctgatcaga tattgttgaa aagcaatacg ataaatcggc
    13321 agactttcta aatcgattcc aatcactact ggttttgcaa tataaaaatc gatcatcaaa
    13381 agaacattgg catactggat catcaggtaa ctaagctcac tatcttcagc tcttttcctc
    13441 ggcaattgct gcaccgctgt ttcctgtaat ttcgccagta actgtgttaa ggaatgcccg
    13501 agcattttct gctgctgttc caaaagccga tcacgctcat agacttcgat ctttccaaca
    13561 aagaaaaacc attcattgtt gatctgggaa atttggtagc ctactgcctt ttcttctttg
    13621 attttcaaac gacgaggacg ataatgccat aacatcgttt cccgaatgta agtatctaaa
    13681 acagcggttg gtaacttttt gctccgtgtc aaatcatact gataaaatat ttcttcgtct
    13741 actatcgaaa aagaaacaaa aaagctgtaa aacatcattg tttcgtatac attcagttct
    13801 gctgctgttc gactaagatg catcacgatg attggatcga ttgcttgata aagccggtcc
    13861 gactgataga gtaatttctt ttctttcaaa gttgcgtacg tacttttttc attcaatagt
    13921 ctttttttcg taatcccgag ccagcaagca atctgtgctt ctgctgatgc cgtaaacgtt
    13981 gtttctaata cacggtccaa tcctagaatg aacggtcttt tttctggtgt attttgcaaa
    14041 aaaagcggtc gctgatcttc cgggatatat tggaatagtt cataataaaa atatcgtatc
    14101 tgtagctctt ctccgtataa ttgaccgttt ttgatttgta atccaaattc ctctaagagc
    14161 tggttcaatt ctttgatctt tcgaaaaaca gaagactcac tgatattaaa cgctgtcgct
    14221 aatcgaacga tggatacttg ctgatgttct aataaataga cgagcatctt aaacttcaag
    14281 ctttcctgca ataaaaacaa tagaattttt tccaaactta catcttcgtc caacacaagc
    14341 agcgcctgat actcatttcg ctgtacttct actttttttt ccatcattcg gccaatcaat
    14401 tcaatatctt ccaaatattg atcaaacgag gcttttgaca gttggacatg ctctctcaaa
    14461 ccattgatag aggctgttcc cccttgcaga acaaggtttc gcaaaatcag gatctgtaac
    14521 tgctcttttc tttctaataa tgcttcaatc ttcataggca gtattcgcca cctttgacca
    14581 caccatatct ggcacacggt attctggctg gaaaaatagc tcatgttctt ctaactgttc
    14641 aaacgtccat ccttctttta ctgcaattgt cagttggtca agaaggtgga acatctcttt
    14701 tttgctttgg agctgtgctc caagaatctt tttttcagaa tcaaatagaa atttgagttt
    14761 aactttgctt tgatttattg ctgtaagctt gtagtctacg tatcctgttt caattttttg
    14821 gggataaaaa atgctctctt cttctgtcaa acctacgctt cctaagaaat agccaaacca
    14881 ctgattgccg actggacgat aggttccctg atcctttgtc tgtgagccac taattgtctt
    14941 gctgacattt cgtgctgtac ggacagcgtt atttactaga gaaacataca ttttttcatt
    15001 tgttggacga aaagttactt ggatcgcatc gccaatcgca taaacgtctt tttgagacgt
    15061 ttgcaagtag tcatctaccc agatcgttcc atcatcattc aatgttaatt gttcttccca
    15121 catctgattg tcggggcgag aatgattagc cagcaaaaca agatcatagt tctcctttct
    15181 tttttccgtc tctacaatag cggtttcatt ttcggtcaaa gacacaactt gttcttctaa
    15241 aaaaagtgaa agcccttgat ttttcatttc ctttactaac ggttctgtca tttcaggatc
    15301 tagataacga aaaagaattg actttcggct ttcgtaaaga tggatttgtt tttttcttga
    15361 tgccaaccct tcagctattt cgattcctgc ttgtcctgca ccaataattg caattttctt
    15421 tgcattcatg atcttttgct gaagagccgt tacgttttgt atctttttaa ctgggtgaat
    15481 aagcgttgat tctgcagaaa tatttcgaaa cttttgattt gaaccagtcg ctagaatcaa
    15541 acgatcaaaa tccaattttt ttcctcctgc taaaacgact tttttttctt tcaatcctat
    15601 tactgtcttt tctgtgtaaa catggattcg atagcggtca gtcagttctt ctttcgtgat
    15661 ccaagaaagt tgtttttcac tggtaacctt tccttttaat atcaaattga acccgctggg
    15721 aatccatcct aacttcgatt gcttttcaat caaatatatt tcacttgttg gcatcaattg
    15781 gcgaagataa atagccgcat gaatccctgc aaacgacccg ccgacaatta caatgttcat
    15841 ctgcattcct ctttcttata aggtgttcta ctgctaactt ctcaatcaga aaacacagta
    15901 ttcataactc gctttcaacc ttatgataca tagcgatgca gcagaatcaa ccctctccat
    15961 cataccgtgt tctaggtctg cttctcaata atgtactagt ggacctaatt gaagagtatc
    16021 atttctgaag agatgtttca aaaaatgtac tcttataaaa gaaaaaaact cgccttttac
    16081 gtacctctgt ccaaatagga aacagaaaag aacgaaaaga cgagttttag cgtgtgtaat
    16141 gtaatagtta gaatagacac atcatttatt tttaattctt taatgcttca acaactgctt
    16201 cgcctaattg atgagcagat tgaggatttt gccctgtaag aagccgccca tcaatttgaa
    16261 ccacttctga aaaagctgct ccagactgat agtttgctcc tttttctttc aaagcatctt
    16321 ctgctaagaa tggtacttca ttcgttgtac catttgcttc ctcttcttca ttactgaacc
    16381 cagtcactgt acgtccagct aaaatagatt ttccagactc atctgtaact ggcaacaatc
    16441 ctacaacacc gtgacaaaca gctgagacca gcccttcatt tcgatagatt tgtgaagcga
    16501 ctttcccaag atctattgct tctggaaaat cccacattgt tccgtggcca ccagcataat
    16561 aaaccgctgc ataatcattt gcatccacat catcaggacg taaggtattt cccaaggccc
    16621 gatttctgaa atcttcatct tcataatagg cccaatcgat ttcatccata ttcaaaacac
    16681 tttgcggttc taaaggtaca tatccgcctt taggactgac aaaatctgca tcgatcccat
    16741 tttttactaa tacatcataa aaatgagtca gttcacttaa ccacaaacct gtaggacgct
    16801 cgatcttttc gtacttttct acatttgtta atacgatcaa tactttcatt ttcccatcct
    16861 ctttctataa aaattgcgtt attgataacg tgctttcaag aaattatata atgggcttgg
    16921 caaataaagc tctttatatg cttcttcaaa aggtttcgtt acaaattcat cctcacggtt
    16981 ttccaataat ttctctgccc agtcaggatc gacgatcatt tgctgtccaa ttgctgcaag
    17041 ttctgcatat ttcaatacct gctctacatc ttcgcgattg cggatcccac cgacaccaac
    17101 taaagggatc tttccttgaa tcgtgttatg gaccacttca agaatcgatt tatcgttgta
    17161 tttatcagaa cgagctattc gatcatacat atttaatgaa acatgcagat aatctagttt
    17221 tgactcacgt aatcgttcaa gaagccagat cgtgtcttca aaacggatac ctggtgtttc
    17281 atattcttct ggtgagaaac gataccctac gatgaatggt tttttagcat attgttcaac
    17341 cgctgagaaa accgttgtta ataattcttc aataaaacga taacgatttt ccaagctacc
    17401 gccccattga tcctctcgtc tattcgaatg cggagagaaa aattgttgga tcaaatacgt
    17461 atttgctcca tgcaactcga ttccatcaaa tcctgattca atcgctctac gtgtggcttt
    17521 gccaaatgct tggattgttt caacgatttc ttcttcactc attgctcgtg gtgtttccgc
    17581 atcaggacgt tttgctgcaa ctgcacttgc agatacaggc tgttctccag ataaagttgc
    17641 tcgacttgtc attcgtccac cgtggaaaat ctgaataatc gcttttgcgc cttctccttg
    17701 gattgctttc gctagctcac ttaatcgagg aatcatttca tcatgagcga tgctgagttc
    17761 acctggccag ccttttccga tatcttggac atttgcagca ccagtaataa cagcaccaag
    17821 acctttagaa cgttgtttat aataagtgat ctcatcttcg gtgatcactc cattgaaaaa
    17881 gctgagttgt gtcgtcatag gcgacagcat caatcggttc ttcaattgaa ctcctgaagg
    17941 taaagtaatt gattgatcaa attgtgacat tgtcattcct cccataattc gctctgatta
    18001 acctatcgag aatcatctat cattaaacca ctcgattagt tgtacatgca actaatttgc
    18061 ttaaaaaatt ttattttttt tgtaacttat ctgttgcatc attcattgtc tgggctaatt
    18121 ctttacttgc ttcatttccc aaaactgctt tgtatgactg atgaagttct tcccagctac
    18181 catcgacttc cttagcaaat tggcgtcctt gttctgtaag ataaactaat gacaagcgac
    18241 cttcgctaac tgttttgacg tatcctttat attctaattt ttcaacaaaa cgagcaatcg
    18301 ttgatggggc ggaatagagc atttgtgcaa tatctttttg tggtattcca tctttttctt
    18361 ttaatatcaa taaaataaaa ccataagttg gtgccagacc tgtccttcta aacgcttcat
    18421 cagctaattt attcaacatg cgatctagct ttttcacgct aaagaaaaga cattcatcta
    18481 acgctttttc aattggcttt tccatttcat cccctccatt ggcgtacata tcatagtatg
    18541 atttttattt tttgtcaaaa aacacggaat aatgtccgcc tcctctgtct agatttgaaa
    18601 aaaagaagta gccatcttca aagaatttcg gcttgttttc gacaaaactc cttctggaac
    18661 accgtgaatc agaggttgtg aaaaagtcgt ttagttttga gtatgaagaa aaattttgtg
    18721 aaaatggctt tccacatttt tgcataaatt tggcttaata ccgaaaaact ggcttttgaa
    18781 caccgtttat atcagaggtt gtgaaaaagc cgtcctgcat caagtaataa gaacagccaa
    18841 acaaaaatag ttcctcatat tttgtcgttt tttgagctaa tgccgatggt cagctgattg
    18901 tacaccgttt attgagaggt cgtgagcctg ccgttgaact ccgagtcaca aggagcaatt
    18961 tcaaaaaaca gcttctcatg ttttatgact atggtgactt gtgatcgagg agttggcagg
    19021 agaatactgt ttattcgtaa agaaggggct gtgacaagac ttttgtcaca gtcccttatt
    19081 tccttttatt ataggatttc gccatttgat tggatgactt gtctgtacca atcaaatgat
    19141 tttttcttta accgtttctt tgtgccattc ccttcgtcat cttgatcaac atagatatat
    19201 ccataacgtt ttgacatttg tgaagtagaa gcgctgacga tatcaatgca gccccacgac
    19261 gtataaccaa ataaatctac tccatcttcg atagcttcct tcatctgctc aatatgctgc
    19321 ttcaaatagt caatgcggta atcgtcttga acggttccat cttcttctac tttgtctata
    19381 gctccgagtc cgttttcagc tatgaacagt ggtttttgat acctatcata taattgattc
    19441 aaagcaattc tcaaaccctt aggatcaatc tgccagcccc attcacttga tggaagatac
    19501 ggatttttaa ttcctccagt tattaagttt ccttggaccc cttccaaatt ttctggatga
    19561 gcactgactg cactagacat gtagtagcta aaagaaacaa aatcaacagg gtaggctgct
    19621 aagatttctt catcacctac ttctattttt aagttgacac ctttttcttc aaacattcgt
    19681 tgcgtatacg ctggataata tcctcttgct tgaacatcac taaagaacaa agaagttctt
    19741 tgctgctcaa ttgttttttg aatatcatcc ggatcacaag aatacggaac aagaagctga
    19801 tagctgatca tacaaccgac ttgtgaacct ttgataattt catgacaagc ttttgtagct
    19861 aaagcacttg ctataaactg atggtgtgca ccttggtatt tcgcttgttc caaatgggga
    19921 tggttttctt caattactcc tacgctaaca aaagggtgat gttttacaca gttgatctca
    19981 ttgaaagtca tccaatattt tactttattt ttatagcgtt tgaatactgt ttcagcgaaa
    20041 cgaacgtaaa actgaataag ctttcgatta ggccatccac catattctgt aaccaaatat
    20101 aacggcattt cataatggga aagagaaacg actggctcaa tcccatattt tagacattcg
    20161 tcaaacactt gatcataaaa aaataatccc gcttcattgg gtgtttcttc atcaccatga
    20221 ggaaaaattc ttgtccatgc gatagacatc cgatagcatt taaaacccat ttcagcaaat
    20281 aaacggatgt cttctttaaa gtgatgatag aaatcatttc cgtgtctttt aggataataa
    20341 tacttgctgt tgggatcctg cgccgctttt acttgctgat gcgtcatcat atgccattga
    20401 tctgtccatt tttctttagg taaatcgctg tcgaatgtat acacatcaga cacagataat
    20461 cctttttcat ctacatccca tgcaccttca cattgattgg ccgctgtcgc tccgccccat
    20521 aaaaatcctt ctggaaattt tttcatatcg atccccctaa acttgctagt tttcttgttt
    20581 gattattttt cttatgacta gtaatgatat ttttgaatct actactattt gtaacaataa
    20641 ccattgtggt cggatcatat ccctctttga ttaattcttg caaatctact tccatcaata
    20701 aatcgccttt acgtactttt tttccatcag atactgcact ttgaaagtat ttgccattta
    20761 aattcactgt atcaataccg atatggatca gtatctctgc tccttcttca ctgactaccc
    20821 caattgcatg tttcgttgga aaaactgttg aaacttcccc gtttacagga ctgtagattt
    20881 ttccgtccgt tggaatgatc gcaaaccctt ctcctaaaga tccactagca aatgttggat
    20941 cgtttacttc agataaatca attatttcac ctgctgcagg agtacttaac actatatcct
    21001 ttgaggattc ttcttcgatt tcatccttgt agccgaaaag aaaagttagc atacaggaac
    21061 tgatcatcgc taagaaataa ctagcaacta gccaaacaaa tccttcacca atataaatcg
    21121 ggaaagacaa aatacttctt gtagcaactg ccaaagcacc ggctcctgcg gcaccagcta
    21181 ctccaccaaa tatagctcca catatacaag caatgtaaaa tggtcgttta tatttaagag
    21241 tcacaccata aacagcaggt tcagtgattc caaataatgc tgtgataaat gcagataaag
    21301 ataaactttt tactttctta tttttcgttt tcaaaaatac cccaagtact gctcctgctt
    21361 gcgcagctac ggcagtcata catgctggac ctagcgtatc acgaccgtag aaagaaagat
    21421 tgttggtcat tactggtaca agtccccaat gtaacccaaa aatcactaac acttgccaaa
    21481 atccgccgat aacagcacca gataaaacag ggctaaagtt ataaacagct aaatatccag
    21541 ctgcaatcag ctgacttgca taatctgcaa ttggacctac aactaataaa gtaattggga
    21601 ccatcacgat gatacaaatg aatggtgtca ataaatttcg aatactttcg tgcaagcttt
    21661 tgcgaatctt aggctcaata taagaaagaa cccatacagc taaaattgct ggaataaccg
    21721 tacttgtata tcttgctaga atcacgggca tccctagaaa tcgcaaggtt atgctatcac
    21781 tatatgcact gacaatcgta ggataaacga gagctgctgc aataaccata gcgatgaaac
    21841 gatctgtatt gaactttttt gcagcagtat atgccaaaaa tataggcaag aaatagaaaa
    21901 atgcatcagc tgctgcattc aaaatgatat aagttcctga ttcagtagtt aataaattca
    21961 aactcgtaca taaggcgagt actcctttga tcaaaccact tcctgccaag acgcctaaag
    22021 tcggggcaaa aacagcggcg atcatgtcta taaaagagtt taaaatgcct tttttctcag
    22081 ttcctctctt tccactagat gcatcatctt ctagtttcat ttgtttggat aatacgtcat
    22141 acacttcatt tactgcattt cctactacga cttgaaacat tcctccagct tcgactacag
    22201 tgatcacacc ttctagttgc tctaccttct ccttatctgc cttcttatta tcttttaggg
    22261 taaagcgtaa ccttgttgca caatggtaaa cattgctgac attttcttcg cctccgacaa
    22321 atcggacaat atctttcgct aattgtgaat agtccatttt tacttccccc ttgttaatta
    22381 atgccgtagc aatccttctt acaagataag tatagttctt agaaaacggt ttctccattc
    22441 aattagtacc ccttctaata gcgaacgaaa ctgtacaata gtgtgtatgg tttacttagc
    22501 cgagcaaatc actcactgct tgaaagaaaa caagtatatc gtttctctta tagtcaaagg
    22561 agacaaaaaa aacacgcttg atcttccttt gctgatgtat aagtggtatc tcaagctgat
    22621 actctgtgat ttttgtttct acttggcttt tgataagaca gcacaacaaa gtctcggcat
    22681 ttaacggaag cataactagg tcagaactag gagagattag ttgctgaatg gaatctatct
    22741 ttttatggat ttttatttga ttttcttctt tgtagtaatg atcaagcagt tttgttttct
    22801 ttttttcttc aactatggtc agcttttcac gcaaaatctg ataaatctcc gaagattcaa
    22861 actcatctag aacactaggt agataaatca aatcatccca ctcaaaaata agctcttgct
    22921 cagtagtcaa aattgcatca tattccgtta ttttgtttga ttgatttttg aaaagataca
    22981 ctggttctat ccggcattct ttgatccaca tgttcatact ttgttgaatc tgttttttta
    23041 gtgtattcaa aatactaatt ggctggttac taacaatcag tatctcccct tgttttcgaa
    23101 aaggattcaa cgcttcggca agatacaaaa ccaaataagc gctttcagct aaattgggac
    23161 gtaattgaaa atgtattggg aagaaacgcc gaattaaata aatttctaaa ggataacggg
    23221 agatgatctt ttgtacaaaa tgattcatgg tattatgtcc ggcatccatc ctcgttttca
    23281 tttgtttgat atgggttagc aaaaattctg tttcatggaa caataacgaa acaggaagat
    23341 ctaaaaaacg aataatcgtt tgctcaaatg ctcttagatt cttctctatt tctttgtttt
    23401 tttcatcatt aatcatcaaa taatttaact ctaatagtcg attttcaatt gcttgttttt
    23461 cttgattgga aaaagaatag ggtagttctg atgataactt ttttgtgagg gtctctacca
    23521 tcggaataaa taatgtatac ctgtttgttt tttctaaaag ttcttctctt cgaagaatgg
    23581 tcaaaacgag ataccgagaa aatcgtatga agtcctctcc tgaaacaata taattatatg
    23641 atataagaac ctcttttaat gtcttttgga cagtcggcat gatattggaa aaaattgtac
    23701 tgatttgttg aggcaacctt gcgtgttgga tcaccgtttc tgttccaaca agagataaga
    23761 aaattctttt tgaattttct tcagcaatga tttttagtcc ttttgtcgac gaaacttcca
    23821 gttctaattt aggattgcgc tcgatccaac gcaaaatcgt ttttatttgt aatgatacgg
    23881 ctgtttttga aagaaagaaa atgtctgcta tcttttgcat actgacatac cctttttgaa
    23941 acaataagta cgtcacaata gttgaagctc ggaaatccat gctggaatag attttatgtt
    24001 tcgtcacctc ttgtatgatt tgctgtttag tttcagattc aattgattct aaaaaataac
    24061 ctgaacgttt atttgactgg ataatgattt tcttctcgta gatagcaagc atatttttga
    24121 tttcagtttg gatcgttttc ttgttggtac ctaaagcttc agctaattct tcgcttgtta
    24181 tccaatgatt cttatttctt aatactacaa gaatttggag ttgtcgttta tttaagtcca
    24241 tttgcttaca cctcctcttt gttataaaca tacaggaaaa acctctcaaa tgacaatagt
    24301 cacttgcaga ggtttaaaaa tcatttgaag aaagatctat attcaaggtt tttaccatta
    24361 gactggataa tttgtttata atattcaaaa ctatcttttt tcaatctcct aaagcttccg
    24421 ctcccatcat tttgtctatc cacataaata aagccatatc gcttcctcat ttcccctgtt
    24481 cctgccgata cgatatcgat tggtccccac cataagtaac caatgatttg gcaaccatct
    24541 tttattgcct cagcaatttc ctgaagatgt atttttagat actcacaacg ttttggatcg
    24601 tggattcgat tcaattcatc tggtgcttca tctagtccaa tgccattttc aactatgaat
    24661 aatggtaatt cataacggtc tgtcaattca ttcatgacat aacgaaaccc tagaggatca
    24721 attggccaac tccaaggttc gggagatttt tccttcaaaa agggattatc gaaaccattt
    24781 gctccgccag tatctacgcg catttctgct tcttttttat agacagcgct acgataataa
    24841 ctaaatgcaa tataatctac cgtgttagct tgcaagtact gttcgtctcc atctaacatt
    24901 actggtgcac agtcttcttc cttccatatc ctgtaaatat aagctggata atgccctcgg
    24961 cacatgacat cggagaaaaa ccatgaattg cgtttaaatt gcagtgaacc aaatacattc
    25021 tctggatcac agtcatatgc ataagtcgct aatgcagaaa agctacacat aatacccatc
    25081 ttagcatttg gatcgatttt ctttaataat tgagtagcac gggcgttcgc tagaaacata
    25141 tgatgacagg cttgataaat ctcttttttt gttagtgtag caatgggttc aagtttatct
    25201 ctagcaggga atgtaaccaa tccagcagct gcataaggca tacgaaaagc attattgatc
    25261 tcattaaagg tcatccaata tttcactttc ccttggtagc gttcaaagac ggtctgcgca
    25321 taaaattcaa aaaagtcgat taattttcga ttttgccacc ccccatactc tcctaccaat
    25381 gctaaggggg tttcataatg actaagagta attactggct cgatatcata tcgatttaat
    25441 gtattgatta gtctgtcata aaatacgaga ccagcctcat ttggcttctg ttcgtctcct
    25501 cttggaaata ttcgtgccca tgagatgctt gttcgaaaag cttttaaacc taactccttc
    25561 agtaaacgaa tatcctcttc aaatcgatga taaaaatcaa tcgcttcatg agaaagataa
    25621 tggagtgatt cgtctaaagc aatctcccaa atcttttttt cttcattcca ctgaatacta
    25681 ggatgctggt tcatgatccc gataagcgta tctgtcacat taggtaattt gccgtcttcc
    25741 aaccatgcac cttcacattg attagcagct actgctccgc cccataaaaa attttctgga
    25801 aatatttcct gatccataac tatctctcct cgctatcaac aatttcttac ttgaagtata
    25861 gctcgattca tcaaaacaag atattctaac tatttcggca gaagagaaga aaaaaattga
    25921 atgctactct tcatttttaa tatttatata taacaaatac ataagacgat ccccgaaaga
    25981 aaacactcgt taaccatcta agcaaacgat ttattttact tccaacacaa atgcttcata
    26041 aggtcgtaaa tcaactttgc cgtctggtaa ctgtttcaga ggataatttg acacaataac
    26101 ccttaaagct tcacgtggaa cagtgaacgt ctgcttttca ctggacatat tacaaataac
    26161 gagccattgc tggtcttcat atatgcgaga ataggcaaag acatgctcag gcgtcttttc
    26221 cactaattca tagtctcccc agacgatgat gtcatgtttt ctacgcaaat cgatcaattt
    26281 cttgtacata taaaaaacag aatcttgatc tgctaacgct tcttctgcat tgatttcttt
    26341 ataatttgga ttgactcgaa gccatggaat tccagtagta aacccagcat gttcacgggc
    26401 gttccattgc ataggcgtcc tagcattgtc tcttcctttc gcattgattg attccaagat
    26461 ttcctctttt gtaaatccag aagaaatcct ttcgttatac atattgattg tctcaatatc
    26521 ttttgcttcc gcgatatcat cgattgggca attcgtcatg cctaactctt ctccttgata
    26581 gatataaggg gtgcctttca tcatatggag caagatagca aacatcttgg cactttcttt
    26641 tcgatacaca tcgtcatttc cccaacgaga aacaattcgc ggaagatcgt gattattcca
    26701 aaataaacta ttccatcctt catcacctaa agatgtttgc catttggata atactttttt
    26761 cagttcagag atactcaagg gtttcaaatc ccatttgtct ttgccttctt gctgatctaa
    26821 tcctatgtgt tcgaattgga agaccatgga taattcattt cttttaggat tggaataaag
    26881 tttagcgatt tcggtagttg ctccccatgt ttcgccaaca gtcatcagat ttttgtcacc
    26941 aaaagttgct tgattcattt cctgaagata ctcatgcagt ttaggtccat tacttgtgat
    27001 ttctttatcc ggttctttgc caatcatatc gatcacgtcc atacgaaagc caccaatacc
    27061 tttatcgatc cagaaattca tcatttgata aatctcttgg cgaacctgtt tatttttcca
    27121 attcaaatca ggttgacgct tgctatacaa gtgcagaaaa tattgccccg ttttttcatc
    27181 caactgccaa gcagaaccac tgaatattga gcgcaatcta ttaggctctt ttccattgac
    27241 cggatcacgc caaatataat agtcccgata aggattttct ttatttttac gggcctcaat
    27301 gaaccacgga tgttcatcag aagtatgatt caccaccaga tccatgatga tacggatatt
    27361 tcttttcttt gcttcttcaa tcagttcttc catttcttcc atcgtaccga attctgccat
    27421 gattgcttga taattactga tatcataccc attgtcatca ttcggagact cgtatactgg
    27481 agaaagccaa attgcatcaa tacctaaata cgccagataa tccaacctgg aaataatccc
    27541 ccgcaaatct ccaataccat cttgatttga atcttgaaaa cttcttggat agatttgata
    27601 aacaactgaa tggtgccacc atttttgttc catg
//
LOCUS       DASHRQ010000033        27251 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.033, whole genome shotgun sequence.
ACCESSION   DASHRQ010000033 DASHRQ010000000
VERSION     DASHRQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.033"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(161..445)
                     /locus_tag="U2M88_001561"
     CDS             complement(161..445)
                     /locus_tag="U2M88_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373948.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(463..1353)
                     /locus_tag="U2M88_001562"
     CDS             complement(463..1353)
                     /locus_tag="U2M88_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEM8373949.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1373..1591)
                     /locus_tag="U2M88_001563"
     CDS             complement(1373..1591)
                     /locus_tag="U2M88_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEM8373950.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1561..3261)
                     /locus_tag="U2M88_001564"
     CDS             complement(1561..3261)
                     /locus_tag="U2M88_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEM8373951.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFRSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3528..4301
                     /locus_tag="U2M88_001565"
     CDS             3528..4301
                     /locus_tag="U2M88_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373952.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="U2M88_001566"
     CDS             complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="U2M88_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEM8373953.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5017..5119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5125..6096)
                     /locus_tag="U2M88_001567"
     CDS             complement(5125..6096)
                     /locus_tag="U2M88_001567"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEM8373954.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="U2M88_001568"
     CDS             complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="U2M88_001568"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEM8373955.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8066..8884)
                     /gene="purR"
                     /locus_tag="U2M88_001569"
     CDS             complement(8066..8884)
                     /gene="purR"
                     /locus_tag="U2M88_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEM8373956.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9051..9875)
                     /locus_tag="U2M88_001570"
     CDS             complement(9051..9875)
                     /locus_tag="U2M88_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEM8373957.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9848..10528)
                     /locus_tag="U2M88_001571"
     CDS             complement(9848..10528)
                     /locus_tag="U2M88_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEM8373958.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10551..11489)
                     /locus_tag="U2M88_001572"
     CDS             complement(10551..11489)
                     /locus_tag="U2M88_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEM8373959.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11734..12597)
                     /locus_tag="U2M88_001573"
     CDS             complement(11734..12597)
                     /locus_tag="U2M88_001573"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEM8373960.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="U2M88_001574"
     CDS             complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="U2M88_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEM8373961.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15453..16259
                     /gene="proC"
                     /locus_tag="U2M88_001575"
     CDS             15453..16259
                     /gene="proC"
                     /locus_tag="U2M88_001575"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEM8373962.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16338..17894)
                     /gene="rny"
                     /locus_tag="U2M88_001576"
     CDS             complement(16338..17894)
                     /gene="rny"
                     /locus_tag="U2M88_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEM8373963.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18305..19354)
                     /gene="recA"
                     /locus_tag="U2M88_001577"
     CDS             complement(18305..19354)
                     /gene="recA"
                     /locus_tag="U2M88_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEM8373964.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19463..20704)
                     /locus_tag="U2M88_001578"
     CDS             complement(19463..20704)
                     /locus_tag="U2M88_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEM8373965.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="U2M88_001579"
     CDS             complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="U2M88_001579"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEM8373966.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21731..22654)
                     /locus_tag="U2M88_001580"
     CDS             complement(21731..22654)
                     /locus_tag="U2M88_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8373967.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(23393..24679)
                     /locus_tag="U2M88_001581"
     CDS             complement(23393..24679)
                     /locus_tag="U2M88_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEM8373968.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24672..25940)
                     /locus_tag="U2M88_001582"
     CDS             complement(24672..25940)
                     /locus_tag="U2M88_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEM8373969.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26192..26653
                     /gene="mscL"
                     /locus_tag="U2M88_001583"
     CDS             26192..26653
                     /gene="mscL"
                     /locus_tag="U2M88_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEM8373970.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26712..27158)
                     /locus_tag="U2M88_001584"
     CDS             complement(26712..27158)
                     /locus_tag="U2M88_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8373971.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         7743 a         5878 c         4727 g         8903 t
ORIGIN      
        1 taataccgag aaaatggttt ttgaacatca cttattcaaa ataaaaaaag attgtgacac
       61 gaattttgtc acaaccttta aatgtattcc tagagcaaag aagctaagcg aagaaatcca
      121 attctgatta tttattgtat ttctttgttc agcatccttg ctaaaagccc atattggaca
      181 aagtattcac tacacccatg atctcatctt ttcgagaacg tgctttttct gtcggatctt
      241 ctacctcttc tgtgtcaatt cgaacaataa atttccgatt catcgtcacg taagcaccgt
      301 cgttgatcaa aataaacagt tcgttctcat tatagtctgc acaggcatct ttccatacgt
      361 caaagccgtt tttttcagaa gggatatccc gtttaaccac agtttttcca cttaattcgt
      421 ggaacgttac gtcataatat ttcattatct ctacctcttt cgtcattcaa tattttgtac
      481 ttgttctctg attttttcta agatcgtctt agactggacg acttgatttt taatttcgat
      541 tgcatttgat ttggaaccga tcgtgttgat ttcccgattc atttcttgga tcaggaaatc
      601 taattcccga cctacaggac attgtgcttc gataagttca tctagttttt gtacatggat
      661 cgccagacga tccagttcct cgtgaatatc tccgcgttcc aacaagatgg ctagctccgt
      721 caacaaccgt tgctcatcga ctttcgctcc aagaaattcc tccagctttt tttgatacct
      781 tatttgaaat tccttttcat agatttctac aaaactatcg agttttttta gtacttcttt
      841 gaattcttca gtattctttt tcaacactaa agctaaagct tgaccttctt tttctcgact
      901 cgcctcaata gaatcaagtg cgcttttcaa tgtatctaga gctaactgtg ccaattcttc
      961 catgctctct tccggttttt cttctaccgt tacgaagttt tcgtccagcg cgaagcgttc
     1021 cgccatccga ctaattgaca cttgattttg actaccgtat cgttgttgaa ttccgtctgt
     1081 caattgagtg ataagtgaat caatcaattc ccagttgatg acgatctgtt tttcgttttt
     1141 gccaatataa gctagattca caaatacttc gattcgaccg cggcttaact gctgtttaat
     1201 aagctgacga aaatcgtttt caacaaagtt caggatttta ggacttcgta tttgaacatc
     1261 tagaaaacgc tggttgacac tttttatttc aatatctaac tgatacgctt ttgtttcgcg
     1321 gatggctttg ccaaaaccag tcatactttt cattcgacag ggtccttcct cattacatat
     1381 tttctattgc tttttgcagt tgttcaaact cttcgttcgt caatgtcagg cctttcccca
     1441 ttttttcatg gtctggcgcc caatctcgaa gatcgaattt tggcgggcga ccattccagc
     1501 tgactaggtt cagttcttta cgccacccct tgttgttttc tgacaatata gcgatttctt
     1561 ctacgatttc atatgaaaat tcttgtgcca tggttgttcc tccttgagat aatataaata
     1621 taggatcagt aatgtgtcaa ctggttgctt aagcaattca tgctgcaaac gatccttgcg
     1681 ttttccttgt agcagataat caaaataaac agttttcgtg cgctcatttt gtattttttg
     1741 ctggtatttt cgataccttc gccattcttc agtacttaat tgaccaattt gtgccaataa
     1801 atcatccggc atacttttta tatcttgttg gcagatcatt tgaatcatca tgtctacttg
     1861 atagatttca ttgggcagta aaaaatgttt ttttgctaaa agttctagca tcgccaatcc
     1921 gcatgtctct ggtacgaccc tgttcttata taaattacgc agtttttgcc aaaattttat
     1981 gcttctgaaa aaagataatt ctaactgaat ttcttttgtt aagtaagaca aaaatcctcg
     2041 ccagtatgtt cttgcaggtt taacagaatg aaatgtatac cagttcccat agtaatagcc
     2101 taatcgtttt ttcatttcca gatagcctaa ttgtaaattt aattggttcc tttggctatc
     2161 aaaaagcaga aagctgccta gtgtccacaa tgtttgacat ttccaatgga taaaaatgtc
     2221 tggcatacga atttttttcg ctgggcctgg cccttgcaca tctacgacaa atgcctctgt
     2281 tgctcctttg ttaatcgcaa tatcaatagg tattttgttg cgatacccac cgtctgcata
     2341 tttatttttt ccgatctttc gataagccat agcgggataa aatgaagcag aggcgagtat
     2401 ccaatctggt atttcttctg tttttgtctg ttggatatca gtcactactt ccgctagttc
     2461 tggcagttta gtagacaccg tatataaacg tattttggga ttatgtttga gtaaggacaa
     2521 atccaaattg tctttgacca tcttttccaa tggagcagaa gaaaccccgc cttcaattag
     2581 tgctgttttc gtcatttggc gtctttctcg atagaagcgt tcacgaaggt cttcaactgc
     2641 agccatttct ggtaacgcaa gcacttgtct tgtcgacagc tttttccata aattcacagc
     2701 ttttttcata tctccttgta aaatcaatgc gccgttcaac gcacctaccg aggtacctgt
     2761 gataatctca aagttgatat catgttcctt cagcgcctgc cacacaccaa tctgataagc
     2821 accgtgcgct cctcctccac ctaaaacaag tgctgtatga tagaccagct ttttatttaa
     2881 ttgtgtggtt ccttgatagc cgtttttatg aaaaatacgc aaccattctt ctgatagagg
     2941 agattgaaat aaaaaacaac agttttcttt aataaaaaaa cgagcgcaag cttccatata
     3001 ccgaaaaaat tttttccatt caaaagtttc accttcaatc aacagattat taaaaataac
     3061 atctcctcta cttttagcaa tagtaaaata gacggtatat tttttcgata ctgctttaaa
     3121 agcacattgc ttttttgcag aacgccaaaa agcgtctcta ctttcttgat gactttgaaa
     3181 aaatggaagg ggttgttttg cttgctggat aggcgaatac agcgacattt tctgtgaaaa
     3241 gtaaactttt tttatctcca tcgcgctctc ctttttcttc aagtatacca gtacgtcata
     3301 aaaaggtcat gagtctcgta ttcgactttg tagattccat aagaaaacaa ttcataaaac
     3361 ctcctttaaa taaaaaaata caaaaatcac aaaaattaat tgaaaatgag agtgttttca
     3421 atgaaaacgt gattttgtaa tggaatttta aaataaaaga aacaagcttt tttaatgcaa
     3481 atgttgtaaa ataaaagaga ttaagaggaa ataagaggag gaacatgatg actgagtcag
     3541 tttatgtaca tattgatacc acaagtaatg ctgttttaac aaaagggctg actgctactg
     3601 actttgcaaa cagtatcgtg cattttcctc aaaacttgct cttgcttgat ccatctgctt
     3661 ctgctggaga atatgaatcc catacagggc tgaaagtaat ccgtggtaca gaaaatattc
     3721 agcgtttctt ttcctcttct cgtaacaggc gtggattcga tgaattgaag tggatcgact
     3781 ttactgattt aacgatgctg aaagaattga cacctttaga aatttcagaa ttattatatt
     3841 ttggtcatat gaaaacacat cttcattcgc cgttttttta taaactacag aataattttg
     3901 tatacttcga tcttaatgat cagctgaatc ggatttatta tcgctattta gatgaatttt
     3961 ataggatttt tgctagtaaa ttaagttcga tcttgtcaga taagatcaat gtaaaaaaat
     4021 cttttttcaa aaaagaaaca cctgttgaaa aaatcagtgt ggaattattg aaagaaatgc
     4081 gtaatttgat gcaggaaggg atcatttttt cttttgatca agttggtttg atcgatggta
     4141 aatatcgtat cccaatctat gtgattgaag attatatttg gaaattaaaa ggcacccggt
     4201 atcgagaaga ggaaatcatc gctactttaa tctactctgt caccgatcga cactggcaat
     4261 tggtagaaga tgccgatctt tcttatctgc cattgaagta gatctatttc ttatcaaaaa
     4321 ataaaaagga aacaagaaac taggacatga ttctgtctca agtttcttgc ttctttttta
     4381 tttacagctc ctgtcgttta tctggcacaa aaatcgaagg agtcgtttgt tggatcccta
     4441 ataaaacgat gatcgttaca atagctgtac tcaacccgct ggcaccattc attacgaaag
     4501 aaaagagcca aggattcatc ccccatagtg catacgctcc ccagaaaacc actcctgcaa
     4561 taaaatgcca aaaatatctg gaaagtgcgc caatcaatgc accaaacacg atgctttttc
     4621 taatttgttt cgtattatcg tctacatagc ctttttggac atttgaagcc atgagccctg
     4681 ctgcgcctgc aaaagtatag gcaatcgggt actcaatcaa cacttgaacg acacttaaat
     4741 aataaacttg tcccaacgga aaatgtagga tgccccaaat gaaacctgcc agcatccctg
     4801 cctttgtccc tcttcttaac gcaaaaagag tcaatggaat ctgccctaat gaaatagaaa
     4861 aactgctgcc gatctggatc ggaatcaacg acaacaccat cgctaacgct gcaacaatcg
     4921 ttccttctac ccaaactcta ttttttccca taaaaaaact tcctcctttg aataaaaaag
     4981 tcacaaagga ggaagcagct ttcgaaaata gtcaaacagc tgtttattca caattcctac
     5041 gctcgcataa ccgatacagg tacgaagggt ttagaaatag attcaatctc agcccaattg
     5101 gactcccctt tgtgagttta tttattattt attttttgtt tcaaacaatg gactaactgg
     5161 tttgttttca tggatacgtt tgattgcatc tcccattagt ccgccaacac ttacttcatc
     5221 gattttgtcg attttacgat cttctggaag atagatcgag tctgtaacaa ctagacgctc
     5281 gatagctgaa tcttctattc tttgtaaagc agggccggat aatactggat gtgtacatga
     5341 agcatacaca ctagttgcac cagcttcttt caaagcattg gcagccaatg tgatcgtacc
     5401 tgctgtatcg atcatatcat cgatcagtac acaagttttg cctttgactt gtccaatgat
     5461 attcattact tctgcaacat ttgcttttgg acggcgtttg tctatgatcg caatcggaga
     5521 tttcaaaaat tccgctaatt tacgtgcccg tgtgacacca ccatggtctg gtgaaacaac
     5581 gacaacatca tcgcctttga ttcctttttc taagaaataa ttggcaataa gtggcgcacc
     5641 catcaaatga tcgactggaa tatcaaagaa accttggatt tgaactgcat gaaggtccaa
     5701 agtcagcatt cttgttgcac cagctttttg aatcatgttt gctactaatt tcgctgtgat
     5761 tggttcccgt gcacgtgctt tacggtcttg acgcgcatag ccataataag gcatgactac
     5821 gttgattgtt tttgcacttg ctcgtttcag tgcgtcaatc atgatcagca actccatcaa
     5881 gttatcattg actgggctgc tcgttgattg aataacatag acatgcgatc cacgaatact
     5941 ttcttcaatg tttacttgaa tttctccatc actaaattga gtaacagatg attttcctaa
     6001 ctcgacgccg actgctgctg caattttctc tgctaatggg cgattggaat ttaacgcaaa
     6061 aatttttaat cttgggtcaa agtaatgttt tgacatgggg acctccactt tctttttcac
     6121 aactctagaa ttctacctaa taaatagtag tcatattttt taaataaatc aagtgatttt
     6181 gcaaaagtct tagtttagat aagggagttt ttttgcatat ccttctttat tgacttgtct
     6241 tgctctcgca attgccagcg catattctgg aatattgtct gtaattgttg acccggcagc
     6301 aatcgaactg tttttagcta cctcgacagg tccgatgata ttcgtgctag aaccaataaa
     6361 gctgtggtcg ccgacagtcg tatgatgttt gttctttcca tcatagttga cgaagacaac
     6421 accacatccg acattgattt cttctcccag cgtagcatca ccaacataag tcaagtgacc
     6481 gactttagtt cctttgccga tttgagcatt tttcacttcc acaaagtttc cgatatggac
     6541 gttttcgcca atttcagctt ttggtcgtaa atgtgcataa ggtcccacat ctgcatgttt
     6601 cttgacatgg ctgctttcaa tgactgagtt ttcaatgacg acgtgatcct caatcctact
     6661 gtctacgatt tttgaatgtg acccgatgac acaatcagaa ccaatgactg tatttccttg
     6721 gatttgtaca cctgcttcga tcaatgtgtc tggaccgatt tctacgcctg catcgatgta
     6781 tgtcgttgct gaatcgacaa agcttacgcc attgaccata tgcatctggt taattcgctt
     6841 tctcatgatc tcatttgctt tagctaacgc gatccgatca ttgactccca ttgattcttc
     6901 aaaatcatcc gtttgataag cagcaactgt atggccttct tcttttaaga tttcgatgat
     6961 atctgttaga tagtattcac cttgagcatt gtttgttcca actttgctta acgcatcgaa
     7021 caaagcttcg ttgtcaaaac agtaagttcc tgtattgatt tcttgtacaa gagcttcttc
     7081 tggtgttgca tctttttgtt ccacgatttt ttctacgatc ccaatatggt cacggatgat
     7141 tcgtccatat cctgttgggt tttctgcttg tgccgtcaag atcgttgcac ttgcattttt
     7201 tccttggtga tattcaaaaa gattgttcag cgtttcagta gtcaataatg gtgtatcgcc
     7261 actgataact aacgttgttc ctttttttcc ttccaagaaa gaagcagctt gcaaaacagc
     7321 atgtcctgtt cccaattgtt ctgcttgtaa cgcatattta ctgcgttctc ctagttgtgc
     7381 tttaacttgt tctgctccgt gtccaacgat cgtaatcact tcatctggtt tcgtttcact
     7441 gacccgatta atgatatgtt ctaccatagg tttaccagaa acgggatgta aaactttata
     7501 taattttgac ttcatgcggg tacctttccc tgccgccaaa ataattgcat aacgtgcgtc
     7561 caactgcgtc actcctaaat atagtttgct gatttcagtt tatcccagta tacccatttt
     7621 ttcaacaaaa caagtgattt tcttttatcc cagcgttttc tggataaaaa aatagataga
     7681 ccataacggg cctatctatt tggtaaccgg tgctctcctg ccaactcctc ggtcacaagt
     7741 cacactactc ataaaacatg aaaagctgtt ttttgaaatc gtttcttgtg acttagagtt
     7801 aaacggtagg cttacaacct cttcattaaa cgtttttcaa tcagctgacc ttcggtatta
     7861 gctcaataaa cggcaaaata tgaggagcaa tttttccgtt tattgagcta atactcagga
     7921 caggacgctg atttcacaac ctctagataa acggtgttca aaagccagtt tctcggtatt
     7981 aagccaaatt tatgaaaaaa tgtggaaagc cattttcaca aaatttttct tactactcga
     8041 aactaagcag ctttttcaca acctcttatt ctgaaaaata atttcctggc acaaccgtaa
     8101 tatttttcgt ttgagtatct acatctttga cataaagcag tgacgtataa tcatcgatgg
     8161 cgcgatgacc gttgaatttt gattcggcaa atacagtgat gccaacaagt tctgattcga
     8221 attcttcaat caagctcttc attccgttga ctgttccgcc gcctttcatg aaatcatcca
     8281 ctacaaggac acgtgaacca cgtttcaaac ttctcttaga cagttccatc ttttcaattc
     8341 tttcagaaga tccagacaca tagttgacac ttactgttga tccttctgtt atttttgagt
     8401 cccggcgtac aataacgaat ggcgcattca gataatagga cacagcttgg gcaataggta
     8461 cgccttttgt ggctacagtc attacagcat cgattttttc accaaggtat ttagaagcaa
     8521 tgattcttcc aacttgacgc agcagttcag gttctcctaa caaatcagag agataaacat
     8581 atcctcctgg aagcagacgg tcttgttctg atagacgttc tgctagagat tcaatatatt
     8641 ttttcgcctc ttcatatgaa atttcaggga tgaacaatac accacctgca gcacccggga
     8701 ttgtttccaa ggttccatac cctcgttctt taaatgtttt cttgatgatt cccaaatctt
     8761 cactgataga agatttagca gattcataac gttcggcaaa gcttgttaag gaaatcaatt
     8821 catgcgggtg attcaataaa tactgtgtca tgtcaactag acgttcactg cgacgtactt
     8881 tcacctattc caactccttt taagaaattt tatcgtttta cattatataa gtttttcttc
     8941 aaaaattcga gcattttatg aaaaaataag aaaatagttc ggtatttatt aaaataaaaa
     9001 gaagcctata aaaaattttt cgtttttata aggcttcttt tctttataat ttagttattt
     9061 tttcttctga aggcaaccaa tattcgtttg atgatattta caatcaggaa caaagcaata
     9121 aagactaatg tgattgttgc tcctggaggt gtatctagat aaaacgagct agttagtcct
     9181 gacagcatcc ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt gaagcttttg
     9241 cctaatcgca taccgattgc tgcgggaaga accatgattg cggatatcaa aagtgcaccg
     9301 gcaatcggga tcatcacagc aatagccaca ccagtaatca cattgaacat catcgacatc
     9361 aaatgaacgg gtagcccatc cacgtgggcg gtatcttcat caaaagtcag aacatacatc
     9421 ggccgtttga ataaaaagaa caatataccg atggcaacga ataagattcc taaaaagatg
     9481 acttgctccc atgtaatggt cacgatcgaa ccaaacagat aggattggat acttgttgct
     9541 gaattccctc cgctcaaatt catcaatact aacgctagcg ccaaaccacc agccattaaa
     9601 atggcaatgg agatttctga ataggttcgg tagatcgttc gcagatactc caatatcaca
     9661 gctgctaata caacgactag catggtcgta agtgtaggat taaggttcaa aaagaatcct
     9721 aacgctaccc ctgctaaaga gacatgagaa agcgtatcag ccatcagcga ttgtcttctg
     9781 atcactaaaa acacacctag catcggggcg attccggcaa tgaagatagc ggcaagaaac
     9841 gctcttctca tgaattcata tgaaagcaac gccattgaga atcctccttt ctcaccagtc
     9901 ggatctgtct gtccgcatac tgtttgatat cttcatgatc atgggtgatc attagaactg
     9961 atttcccatg gacatgcgca ctatgtcgga gaagacgata aaattcattt ctggattctt
    10021 catccatacc tgttgttggt tcatcaagaa caaataagtc agggtcagta gcaaaaatac
    10081 gcgctaaact gattcgctgc ttttgtccgc cagacaattc acctattcgc cgatttcgca
    10141 tatcccacat ccctacagct tctaaagcac gctgtacatg ttgatgatct ttttcagaga
    10201 gacgcttgaa ccagcggcct cttggatatc ttcctgaacg gactaattcg atcaccgtac
    10261 taggaaaacc tgcattgaaa gatgcaactt gttgaggaat ataaccgata cttaattttt
    10321 ttccttcttg atttttctca gcaatcttta tcattccttt tgaaggtttc agtaatccta
    10381 acgatgcttt caccaaggtt gacttggctg ctccattttc tccagttaaa atgacaaatt
    10441 cacccgcatc tacatgataa gagacatctt ctagtacagg ttcatcatca tagtaaaagg
    10501 tcaaatcaga tatttcgata taacgcacct tacaacctcc attctaaaac ttaattgata
    10561 ctttctttta aagctttcag atttttttcc atgacagaaa tataattttc gcctgctttg
    10621 atttgttcat tagtcaagct ttctaaagga ttcaacactt ctagcttcac tcctgtttca
    10681 ttcgacaatg tctcagctac cttcgaagag gcattttctt caaaataaat cactttgatg
    10741 ttgtttttct tcacaaattc ttttagctca gctaagcgac ttggtgtggg ttcctgatct
    10801 ggtgaaacgc ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa ataagcaaaa
    10861 gcagcatgct gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc ggagtatttt
    10921 tgatccagtg cttcaagctt tttgatataa gcatcggcat tcttcgtaaa agtttccttt
    10981 ttatctggat atttttcgcc taattcttca gcaatcgttc tgacttcttt tatagctaat
    11041 accggatcga gccaaacatg tggatcgtat tcatgtgaat gaccatgccc ttcttctgat
    11101 tcttcatgtt cgtctgcttc ttgcgattct aaacggtcga ttcctttagc tgcttcgatc
    11161 atcaaggtct gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa ttccatgtct
    11221 gaactattat acacgaaaac atctgcatcg gaaatttctg ctctttcttt tgcacttggt
    11281 tcgaaatcat gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc ttcatcccct
    11341 acgatctctt ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt caaatcctct
    11401 tttttgtctg cttccttgtt cgtatttcca catgctgcaa acagtaaagc acctatcatt
    11461 accgtagctg tcaaaagata ttttttcatt tatatgctcc tttcaatgaa caaatcggaa
    11521 ttttacgatt tattaacgtg aactaatgta tcaaaataaa aaaacacagt caatagaaat
    11581 ccatcaattt ctgatttaag catgtatatt cttgattaaa tcgtaaaatg gatgagttgc
    11641 tgctcatatc tagccatttc actacaataa aaaactgaag aataccattt taggattcct
    11701 cagtttttca tcacctattt tacttcttat attttattgc agggttcgta ctaaataaac
    11761 ttcctcacaa aaacctttta acccattata tactctctgc gctcttgaat aatgttgaca
    11821 caaggcaaaa acggttggtc cgcttccact catcaatgca gcatctgctc cgtacttgat
    11881 catacgctct ttgatttgat ggatgactgg atgccgcttt cccgttatat cttctaaact
    11941 gtttcccatt tttttgatca tctgctgata atcttgctct cggatggctt cagcaagtcc
    12001 gtggatgtct tgatgttgga tggcttgcgt atctacttct ctaaacactg tacgtgtaga
    12061 gacactcatt cttggtttga ccaacacaac ccagcactgt ggcatagaag gtaatacttg
    12121 gatttcttcc cctttgccac cgacaaatgc cgtacttccg tagacacagt aaggaacatc
    12181 tgtaccgact tccaaaccga tttctgccag ttcttctaac gaacagccca gattccataa
    12241 ttggttgatc cctcttagag cagctgcaca gtctgtactt cctccgccta atccagctgc
    12301 tactggaata ttcttcttaa caaagatctt gacaccggtt ttgatattgt atttattttt
    12361 tagcaataaa gcagcttgat aaacattgtt ccgttcatct accggtaaaa atgctttatt
    12421 tgtttctaca atgattttgt cttctgttat ttcttcaaaa aatagatgat ctgccaagtc
    12481 tacgctcgac atcaccattt ccaactcatg atacccatct tgccgtctat ataatacatc
    12541 cagtcctaaa tttatcttgg ctggcgcttt ttcaatcaat tccatcctct cacccattcg
    12601 aaaagtctaa gtcgcaagta ttctatcata gggaaaaata aaagtatagc ttatgtctgc
    12661 ctagtggttg attttgtcat cgtttcaaca gctttcacag aattttctct tcataatcca
    12721 aacaagttct agtgattctt ataattctgt gagcacttct tctgccaaac tcggaacatt
    12781 tcctttttct tttcctatca gactgagtag ctgcatggta gcaaatccga aattacttcc
    12841 tttcctataa gcaaaataac gtccttccca atgatcgaca tattttaatt tcttttcata
    12901 agcagactgg aaggaataga aaaaattacc ataattatat acgatatgca tgactttttc
    12961 ttgaaagaaa gaaaaatctg tttctccaac attagctaat ggagctaagc ctagattggc
    13021 ttccgagtag cccattttct cgtatacaat aaacagattg gctaacagat agtcagctaa
    13081 ttcctcagat tctttaggta agacccgcaa caaatcatat cccgcctgct tcttcgtcat
    13141 cggctgagcg gtgatgaagc caacgacttt ttgatcttta cggacaatac cgacaccgct
    13201 agataataca tatgatttat caaatcgtcc cacagagaag tatttttctc gtgcaccttg
    13261 aagccatttt tgtgaaactt catttacagc ttgatgtaat tcttccggca gttgatcgta
    13321 ccaagtaaaa tggtatcctt ctttcatcaa acgttgatat tcaagttgct gactcagccg
    13381 agacaagtct tctgttgcta gatcaacaat ccctgcttca cctactttcg tgaagtgaaa
    13441 acccaattca tgaagcatca ctacgtagct ttctgatatc ttataaaacg caagctgata
    13501 gcctaaacaa tctgcttgtt tcataaactg attcgttgct gcccgcatca ttttaggatt
    13561 gccaactgga tctcctaaaa caaagcattt gtttcctttg atctgataac caaaaacgac
    13621 ttgatcttgg tcgtcttctt ggtaataaaa ataactgtaa cctgacaagc gaagctgatg
    13681 gcttgtagct gtgcctccgt atgtttccaa taaacggtca aaacggtctt tttcgtactc
    13741 actgccaagc ttcgtagtat cttcagctaa atattgataa agcgccacta aagctagcaa
    13801 tgagatgccc aaaccaataa gccctgagaa ccacacatca tctgaaggaa acaaaataaa
    13861 atgatttcct gttattcgac cgactaaaaa gcgtccgctg tgatagcctg caattgcgta
    13921 aacgataaac aaaaagccga acaagctagc atcaaagatt atagctcccc aagaataaac
    13981 gaattttgtt cgataaaatt cttttctgga taaccacaca gctaacagaa tcaagacata
    14041 taccaatatc aactgccaag ataacgtacg tgtgattgta tttaaaatac caaaagatag
    14101 taaagcaatc gtaggcagaa atgccttttt gacttttact gaaagtgctc tagcaaggcc
    14161 gagtaataaa aagccaacca atagattcac tgtctgatct aaaaaattga atgagaacgg
    14221 caataatact tgaaatagcc gactgattgc agacagatta gtgattgttg atagaagtac
    14281 catcatgatc cccgcaaaat agacagcact tacgagcacg atatgcgcaa ccttttggaa
    14341 aaacagtcgc ggaatgttat ctaaaaatcg attgattttg actcctgtat gtgtgatgaa
    14401 caatagaagt cccgtcataa aaggtagaac ataataaaac aaccgataat ataatagcca
    14461 aactactgct acatcttgcc gaacaggcaa ttgactcatt cctaagatca tcaaaacgtc
    14521 aaaagtcccc attccacctg ggaccatcgt cagcattcct atcaatgtag caatcatgaa
    14581 cataggatag atcgaaagca tcgaaatatc tattctcatc aacttgccga tgatcaaaaa
    14641 taccgcaagg gctcccgtcc attgtccaaa ggatgccagt agcagaccaa gcaagccttt
    14701 tggataaaac tcgaagaaca atgtctttct ctttagataa gcaaaaaaga aaatgactgg
    14761 tgctaaaaaa ctaccgccga aaagccagat ccaatagctt cgaaaaatac tttctgactg
    14821 aataaaaaag acatcaataa atgtaagaaa cgaccataca gacaaaccag tcagcataaa
    14881 tagtgcgact tttgatacag tggctaaaac catttttcta tttgtctgct ttccataaaa
    14941 actagcccgc agacttgctc ctaccacacc accaaaaccg gcgagattgt tgatcgtgtt
    15001 tgttacccac gcagcaagaa gaaactcttt tctttccatt tttggttttc cttgtttttc
    15061 taagactctg atcgtcaccc aatcatacaa caacatcgga gtgacaccca gcagtccagc
    15121 cagaatcatg agcaggatcg tcatgcggct ttgttgtccc atcctttgcc aaatatcttg
    15181 ccaactcatg ccattagcaa tatttgcgac ttgatttgcc acaaagacta acacggagcc
    15241 gaaaaaaatc agttttaaaa tcagactatg ttcctttatc cattgcagca tcaagtatcg
    15301 tttatttttc acattttccc attcctaact tgtatacttt tgtgtcatac cccaatgtta
    15361 tcttatttat tttaattttt ccactttaga cctgttcttt gcttgcgatg atagaaaaag
    15421 aaagatatcc taatgctaaa ggagaggata acatgaaagt aggatttatc ggcacaggga
    15481 atatggcaca agctattatt gatggtttat taaaaaaagg aacagtctcg aaagaagagg
    15541 tgttcgtttc cagtggacat tttgaaaatg cacaaaaatt tgctgctgaa actggcgtcc
    15601 atgcatgcaa aagtaatcag gaagttgcat cacaatcaga tatcgttctt ttagccgtga
    15661 aaccaaaaat cattgaaaaa gtattggaag aactaagaga atatagccag aaagtcttat
    15721 tcatttctat tgcttcagga aaatcattaa cagatttaga aacagcgctt gaagatcctc
    15781 aggcctccat cgtacgtgtg atgccgaatg tcaatgtcag cgtaggagca ggcgtttctg
    15841 cgatttgcaa aaatgaagct gccacggagg aacagcttca gcaagctacg atgatttttg
    15901 aagcaatcgg cagcgtctat cctttagcag aatcagattt cagtacattc attggccttg
    15961 ctggcagctc acccgcattc gtttacatgc tgatcgatgc gatgggacgg actggtgtac
    16021 tgaatggttt gtccaaacag acagccactg agattgctgc aaaagcaatt atgggttctt
    16081 gtcaaaaatt cctttcaagt gataaaaatc catgggaact tgtagaccaa gtttcttctc
    16141 ctggcggtac aactgttgca ggtgtagttg ccttagaaga agcaggattc atcccagcaa
    16201 tcatcaaagg aatcaatacg acgatcgaga aagatcttga gatgttgaat aaaaaataat
    16261 gatagcaatg ttttttcgtg ccttacaaaa aaagaagagc tgtggctttt gccgcggctc
    16321 ttcttttgta ttttttatta tttagcataa tcagttgcac gagtttcccg aataacgatt
    16381 actttgatgt gtccaggata atccagttct tcttcgattc gtttacgaat atcacgtact
    16441 aaacgaacag cgtctaaatc cgagatttct tctggcttga ccattacccg aacttcgcgt
    16501 ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat attttccaga
    16561 cttcttaaac gacggatgta gttttctaat gattcacttc gcgcaccagg tcttgctgct
    16621 gagagcgcat ctgctgcagc tacaaggaca gagataacag aagtagcttc cacatctcca
    16681 tgatgagaag cgatcgcgtt gattactact gggttttctt tgtacttggc agctaattcc
    16741 gcaccgatct caacgtgaga tccttcaatc tcatggtcaa gtgctttccc aatatcatgg
    16801 agcaacccgg cacgttttgc taattggata tcttcgccaa gctctgctgc taagatacca
    16861 gctaattttg caacctcgac cgaatgcttc aagacgtttt gtccataact agttctgaat
    16921 cgcagacgtc cgatgatctt gattagatct ggatgaagtg tatgagcacc gacttcaaac
    16981 gctgcctctt ccccgtattc gcgaatacgt tcatccattt ctttacgaga tttctcgacc
    17041 atttcttcga tacgcgctgg atggattcgt ccatcttgta tcagtttttc caaggtcatc
    17101 cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa caactgcttc cggtgtatca
    17161 tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc gaatattacg accttctcgt
    17221 ccaatgatac gacctttcat ttcatcattc ggaagggtaa cgactgaaac agtcgtttct
    17281 gaaacttgat ctgctgcgca gcgttgaatc gctaatgata ataagttttt cgctttgcga
    17341 tctgcttctt ctttggcacg ctgttcagat tcttttacca ttagagttaa ctcatgattc
    17401 agctcttctt cagtcgattt catgatgaca tctcgtgcgt cctcttttga taaaccagca
    17461 attttttcta gttctttttg ttgatcttca atcatttttt caacgtcttt ttctcgctct
    17521 tcaattaatt gctgttttga actaagtgct tcttctttac tttcaagtga gttctcgcgt
    17581 ttttctaacg agtcatcttt acgattcagc gtttgttcgc gttgaatcaa acgattctct
    17641 tgagatttta attctaactt actttctctc agctcacttt caacttcaga acggtatttc
    17701 tgattttctt ccttagcttc taacaatgct tcttttttta gagtttctgc ttcttttttt
    17761 gcactatcga tgattccagc agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt
    17821 gactttgcga ccataaaccc taaaccaaga ccgacaatta aaccgatgat agcgaggaga
    17881 atgttgaata ccataaaatc cacctccata ctatcttttt catttttatc atctatacag
    17941 cttctatgat aaattattct aaactcgtca atatgcatct tcgcatatat gtgtacaaaa
    18001 aacatgcaca actttattct aatgtttgta ccccgtagtg tcaactttaa ataaacaaag
    18061 aaacggcttt ctacttattc attaaacgat ggtttttaaa ataaatgaca ctagtttcat
    18121 ataggtaaac ctttaaattg agaaactttt ctattaaatt attctttgta gatgaacaga
    18181 tatttttatt ttatgtaccc actcgtataa ttaaataact cacgatttct ttattcaata
    18241 aaaaaacggc aggtcaagga gagtacctac tcttcagacc agccgttttt gtttatcata
    18301 atttttattc ttctactaaa ggcaattctt cttgtccttt ttcatcttct gggacagcga
    18361 cttcttcacc aataccataa gctgctctaa ctagagcaga aacttctgcc atcatttcag
    18421 gatgattagc catatagatt ttagcgtttt cccgtccttg accaatccga tcttctttat
    18481 aagaatacca agcaccgctt ttatcaacaa tgtctttttc tactgccatg tctagaagtt
    18541 ctccttcttg tgagatccct aaaccataca tgacatctac ttcagcgatt ttgaaaggag
    18601 gtgctacttt gttttttacg actttgatct ttgtacggtt accaacaata tctgtacctt
    18661 gtttcaattg ttctgcacga cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc
    18721 gtcctccagg agtgatttct ggattcccaa acatcacgcc tactttttca cggatttggt
    18781 tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc agagagttta cgcaatgctt
    18841 gagacatcaa acgtgcttgt aacccgacat gtgagtcacc catttcgccg tcgatttctg
    18901 ctcgtggaac taaagcagca actgaatcga ctactacgat atctacagcc ccacttgata
    18961 ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt
    19021 catcgatatt cacacctaat ttttgtgcat attgcggatc taacgcatgc tcagcatcaa
    19081 tgaaagcggc cgttccgcca tttttttgta cttctgcaat agcgtgtagt gcaactgttg
    19141 ttttacctga actttcaggt ccatatactt cgatgatacg tccacggggg taaccgccga
    19201 cacctaaagc gacatctaac gcaagagagc cacttggaat tgtagagatt tgttggtcga
    19261 ttttttcgcc caatttcata atagagcctt taccatagct cttttcaatt tttttcagtg
    19321 cagcatctaa ggctgcttta cgatcatctg ccaatacgat aatcctcctt atatttacaa
    19381 atcagagcaa catttcatta tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa
    19441 accgaacaaa tattcgcatt tatcattttc ttaaaagctg ttttctcaaa agatcaaatc
    19501 cttgcataac cgcattatat ctgatagcag cacgatctct ggcaaagtga tatttttccg
    19561 aaaccacgcc gtctttagat gaaatagcga tccatactgt tcctgcagat tgaccttcta
    19621 acgtgtccgg tccagccact ccggtgaaag ctacactgta atctgttctt gctatctcac
    19681 gtgcttgaat ggccatctgc tccacacatt ctttgctgac tgttccatat tctgctaata
    19741 gctccggatc aatctccaga aaaccagctt ttgtttggag cgagtaggta acgaatccgc
    19801 ctggaaatac ttcagataca ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg
    19861 tcaaactttc cgctgctgtt acagtctgct tgttttcttt cagtagttcc actacgactt
    19921 ttgctagcga attatcatcg ccgtatccat aaaaatattc ccctactcgt tcttggattt
    19981 tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt ttcactgtca
    20041 gtcgtaatgt cacttcattt ggtttggcat atggtgcgat cgttggattg atctgtgtct
    20101 caatcagatc ttttagctca gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa
    20161 ctttagaaat tagtttttct tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt
    20221 ccacaaacat tggtttcaac tcattaggcg gaccgggaag aaggatatac gcatgtgtat
    20281 ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt tggtagagga accccgcctt
    20341 cgataatctg ggactgttgc agattgtttt tggtcatttt tcgatgggtt gtttcaaaat
    20401 atgccaataa ttttttgtat ccttccgtat tttgtatgag agacttaccg aggtgagccg
    20461 cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa tccgccacat aagagtatga
    20521 gttcacttct tgtttcagct agagcaatca actcttctaa acgctgctga ttatctccta
    20581 caactgtttg ataataaact tcgataccta gatcagctag ctgttctgac aagaaagtgg
    20641 cattcgtatt cacgacttgt cctagtaaaa gttctgttcc gacagctatt atttctgctt
    20701 tcattacttc ccgctccctt atatagtata cgctgtattt ttatattttt ttatttaaaa
    20761 agagggcaag gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt
    20821 tctcctgcca aaacctcggt cataagtcac aatatacata aaacatgaaa agctgttttt
    20881 tgaaattatc ccttgtgact cagagttaga cggcaggctc acaacctctc cactaaacga
    20941 tacttacatt gatcctttga acacaccgct gtttttgatg aagtaatcca cacctgaata
    21001 gatagtaaag atcagacaag cataaagcat gatttgatct actggaatac cgatcaggct
    21061 gaatgggata ttattgataa acagcaagat aatagcaacc atttgtgtag ccgtcttgac
    21121 ttttcccggc caagctgcag ccatgacttc tccatgttcg acaagtagta aacgcaagcc
    21181 tgtgacggct aattcacgac agacaatgat cgctactacc caaccagggg cctttccttg
    21241 gtcaaccaac atgataaaag cagtcataac tagcatttta tctgccaacg ggtccgcaaa
    21301 cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta accagtctgt
    21361 gatactagct aaagcaaaaa taactgcacc tactagctgt gtgacagcca gtgtggtgtc
    21421 tcctacgtta aattgtcccc aatctaaagg taaacctaca accaaaatga aaatcgggat
    21481 catacaaatt ctaagtacag ttaatttatt tggtaaattc acactatcga ctcctttctt
    21541 ccctattcta cgtgactatc agcgaagttt ccaccgatat tatttacttt tccaaatgaa
    21601 tttattcaaa ggagtatttt tattgaaatt gatctttttg aattaacgaa gaaggaccgc
    21661 gacacagatt tgtcacgatc cttctttttt atacctacaa gtaaacagat gcgtaaacta
    21721 atttatacga tcattctgcg tattgaagag taaggttcac attttttgga ctctgattgt
    21781 tttcccctgg attaactggt acggattctc cattcgcttt gatcgttgca ttgcttgctg
    21841 caccaatcgt gatggtcgca tttgttgctg tttccggtaa cacggtactc tgtgtttcat
    21901 ttgctgccag tgtcccttga taaacatagg cattgtctac tagcacacct aaccatacac
    21961 gattgacagc agtaaattca agggttacag gcttggtagc gtctgtgata tttactgcga
    22021 tggcactgcc agaatcttct cctgtagcta ttgccatttt cttttcttca cttgattttg
    22081 ttgttgattc tactgtagaa gactctttgg tctgcgtgct ttgctctgag gaagaggact
    22141 cctcgactga tccttctact ttaagcgaag aagctgtgtc tccgattatt ggatcagctt
    22201 gacgatcttg ccaagtcata tagcccacga caacgacaat cgccaaagca accaatccta
    22261 ataaaatgac aggaagactt gtccaaaagc gacttggatg cgattcttcc tgatgcattg
    22321 cttttctaga agtacttact gtttcaggct gcggacgttc agggaccgat cctgtcaatt
    22381 tgcttttccc atcaaaaaca tcgactaaat ggtctccatc ttctcctaca gcttcagcgt
    22441 attgccgaat aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt
    22501 cgattgcttc caaataacgt ttttgaattt ttgtaatctg ctgtaattca tctaaagata
    22561 tttttttatt caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt
    22621 catcttctcc agtctgtttg attctccgaa gcatctgctg ttgcaacgaa agacgttatg
    22681 tttgggtgct gtgacaaaag tcatgtcaca gctccttttt tccgaataaa cattgttctc
    22741 ctgccaactc ctcggtcaca agtcacaata tccaataaat ataagaagct gttttttgaa
    22801 cttgttcctt gtgactcgga gttaaacggc aggctcacga cctctttctt aaacggtgtt
    22861 caatcagctg ttcctcggta ttagctcaaa aaccggcaaa atataaggag atatttttcc
    22921 gtatgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttcc ttaaacggtg
    22981 ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacatta agaagctgtt
    23041 tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct cttggtacac
    23101 ggtgttccag aagtagcttc ttcggcaata agccgaaata tcacaaaaat ttgagaagca
    23161 attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa cctctttgat
    23221 tacttcgttt gattttattt taatataagt tgattaaaag agagcatgat ttgttttatt
    23281 ttctcactaa tttttccaac acaactaaga ataccataaa agctgatttt ggaggaggtt
    23341 cctactacaa gtctaaaacg aaatttaaga cttttcctca taaaatactg gtttattttg
    23401 gtctcatgta aaactcgctg aaggcctctt cacttataaa tgcttcccca gctgccagta
    23461 cgtctttcat ttggatcgaa tcgatgattt caggaagatc aaataaagtt cgatcgccaa
    23521 ataaactttg cgtaaattga ttggcaatat attcgatcga atttaatgat tggaaatatt
    23581 gtccaagcat tttcttcttc agcaaatcga gatttgtttc tgacacttcc ggatcatcag
    23641 cgaatccaag aatgatttct ttgacttttt ctgctgcttt ctctggttcg tccgtatctc
    23701 cgctgaagtc cgcaaagtga aattcacgat ccaagctgaa ttcaaaacca aagctgtcat
    23761 cgataattcc ttgattgtac atcgctagat agtttcgaga agtattgcct aaaatcattt
    23821 ggaacaacag attgattgct gttttatagc gtaacagttc tgttccttct tgcggcaatg
    23881 tgtctaatcc tttaatacca agtacaaatt tatctcgtgt aatggctgct tccaatgcac
    23941 tttgtttgat gatttctttt gtgttttcag ggaaataacg tacaatttct tgctttggag
    24001 ggaaattttt tgcttcttgg ttttcccgaa taagtttcat caatttctca ggctccatct
    24061 taccaactac aaataaaacc atattgcttg gctgataaaa cgtccgatag cacgtataca
    24121 agtcttgagc tgtgattttg tcgatacttt caaccgtgcc cgcaatatcg atatgcaatg
    24181 ggtgtgtagg atacaagtta tttagtatgc caaaaaacat ccgccagtta gggtcgtctt
    24241 catacatttg gatttcctga ccgatgattc ctttttcctt gtttacagtt tcttctgtga
    24301 agtaaggtgc ttgtacaaaa tcgatcagag tcgtcagatt cttttctacc tgatccgtcg
    24361 tagaaaacag atagctggtt ttcgtaaaac ttgtgaaagc attggcggaa gctccttgtt
    24421 tcccaaataa ttgaaagaca tcaccatctt ctttttcaaa caacttatgt tctaaaaaat
    24481 gggcaattcc atctggaact ttcacctttt ctttttcacc atagggaata aattcgttat
    24541 cgatcgaacc ataattcgta ctgaacaaac catatgtctt gtgatagtca tttttaggca
    24601 acaaatacac ggtcaaaccg tttggcagta cttcatgata aagtgtttca ttgatttgct
    24661 catattctgt tttattcatt tgcatttcct cctgctaaaa agaagattgc ctgcaattcg
    24721 atttgctcag ctacttgttg tatctctttg atcgttacgc tctccatacg tttcaaccat
    24781 tcttcatcag ataattttgt ttccggcagc cagctgtcca aaaaagaagc ttcgattgcc
    24841 gcttgcggag agtctagaga aagcagatat tggtttctca gcattgcttt tgtctgagac
    24901 agttccaact ctgttatttc accatttcgc aaactttcta attgctcgtg aatcagatga
    24961 agtacttgat ttcggttctt ttcatcgata ccggtttgga cactcatgaa tcctcggaac
    25021 gtatccacgc tactagaggc ataataagct agactttctt tctctctgac attcatgaaa
    25081 agtttagagt gaggaaaacc gccaaataaa ccgttaaaga ccattagcgc aaaacgttct
    25141 ggttcatcgt aatacacatt tgtctgataa gctaggttca gctttgcttg aacgattggc
    25201 tcacgtactt gtcgttcctt cacgatattc acttgaggtt gggtataaaa cagatctgga
    25261 taaactgttt cccgcgactc aaatggtaat tgcccaactg cttcagctgc tttgtccgga
    25321 tcaacatcgc cgacaacaaa aatgtcgatt tgatcatttg ccatcatttc ttgatagtag
    25381 gacgctagcg aactagcagt gatcttgtcc aaatcttcga ctacgcccaa ccctgggatc
    25441 ttttgttccg ggctgttttc aaaataaagt tcttgcaagg cataggaagc aaaagtttgt
    25501 ttatcttcct tcaaactttc caaatataga cgcagattgt tcttttccaa gtcaaacgtc
    25561 aactgatcaa actgatgatt tttaatattt ggataaaata aaacctcttt taaaaaatct
    25621 accgcttgag aaaaaaggtt tggatcattg acatattcac cattcacaaa actgatccct
    25681 gcgtttagcc aatgtaaatt tccttttttt ctaactgata atccaaagct tgcgccataa
    25741 agctctgcaa gttgcgcact taactttgtc tgatccggat aattcaaact atttgtttcc
    25801 aacatgctgc tgagaagtga acgtttcgtg atgacttctt gttgcagacg cgcagtaaaa
    25861 cggataaaaa tccggatcgt tttgtattgt tttgttggta agacatgaag attcacgcct
    25921 tctgccaatt gatacaccat atttatatgc tccttttcac agttgattgc ccgcttactc
    25981 ttttaatggg tcaacctgcc ttttttactt attcctctgc ttgaaattta taagttcagg
    26041 caattcgccg ttttttctaa atgatgttac actttatagt atagcataga tgttttttat
    26101 caaaagaaac agacggctaa aagcgtatat tgttttttta ttttttattt ttcgatatac
    26161 taaagacaaa taaaagaagg gagggtcatt catgatcaaa gaattcaaag agttcatcat
    26221 gcgtggagat gtcttggatc ttgctgtcgg cgttgttatt ggtagtgcgt ttacaagcat
    26281 cgtaaaccaa gtggtgcaag gcttgatcac accactaata ggttggtttg tcgcaatgat
    26341 cacaggtact tctgatttag aaggagcact ttcaattctt gactggtcgc cgacaaaagg
    26401 cgtcacattt gcgtttggta acgttattag tgctatcatt acatttctga tcactggttt
    26461 tgtcttattc ttagttgtca aagcggcaaa ccgtgcgaag aatttacgct ccaaagaaga
    26521 gcaagtggaa gtggaagaaa ctcctactgc agaagattat cttagtgaca tacgcgatct
    26581 tcttgccaaa caagtggcag aagacaatac acatccagta gagcaaacaa atctttccga
    26641 tacttctaaa taataaagaa tctaaaaaaa agaaagccga ttttcagtgt cggctttctt
    26701 tttttagatt cttaacttgc tacttgttcc tttttcatgt cgatatgtgg aatatcatct
    26761 tccaaataca catctgagat tgccttgaat ccaaacgact cataaaaagc ttgtaggtat
    26821 tcttgtgcgc cgatcacgat tggtttttct ggaaaatttt tctggatcat gttaatcgtt
    26881 tcttgaacaa tctttcttcc taaaccatct ttacggttgt cctgatgaac aaggacacgt
    26941 ccaaaatgga tactctccgc ttcttcatat actcgcgtgt aggcaaggat cttcccatca
    27001 gtatcttgaa aaaacgtatg ccaagcttgt ccatcaatgt catcaacttc ttgataagga
    27061 cactgttgtt ccacaacaaa aactgcgatt cgttctttga cgatcgcgta aaactctgct
    27121 gttgacaatt cttcaaattt ctttacctga tattccacta agcaaacgcc ccctgctttt
    27181 ttattcttag cataccgatt cgcacacaaa aaagcaaacg aaccttacga atctcaggct
    27241 cgtttgcttt t
//
LOCUS       DASHRQ010000034        24271 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.034, whole genome shotgun sequence.
ACCESSION   DASHRQ010000034 DASHRQ010000000
VERSION     DASHRQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.034"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..572
                     /locus_tag="U2M88_001585"
     CDS             <1..572
                     /locus_tag="U2M88_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEM8373972.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="U2M88_001586"
     CDS             576..1217
                     /locus_tag="U2M88_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8373973.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="U2M88_001587"
     CDS             1214..2131
                     /locus_tag="U2M88_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM8373974.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="U2M88_001588"
     CDS             2128..2799
                     /locus_tag="U2M88_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8373975.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="U2M88_001589"
     CDS             complement(3340..3828)
                     /locus_tag="U2M88_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEM8373976.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="U2M88_001590"
     CDS             complement(4029..4295)
                     /locus_tag="U2M88_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEM8373977.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="U2M88_001591"
     CDS             complement(4295..4558)
                     /locus_tag="U2M88_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEM8373978.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5034..5219
                     /locus_tag="U2M88_001592"
     CDS             5034..5219
                     /locus_tag="U2M88_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEM8373979.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5361..7184)
                     /locus_tag="U2M88_001593"
     CDS             complement(5361..7184)
                     /locus_tag="U2M88_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEM8373980.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="U2M88_001594"
     CDS             complement(7782..10487)
                     /locus_tag="U2M88_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEM8373981.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="U2M88_001595"
     CDS             complement(10793..11740)
                     /locus_tag="U2M88_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEM8373982.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="U2M88_001596"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="U2M88_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEM8373983.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="U2M88_001597"
     CDS             complement(12375..12737)
                     /locus_tag="U2M88_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEM8373984.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /locus_tag="U2M88_001598"
     CDS             complement(12748..13869)
                     /locus_tag="U2M88_001598"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEM8373985.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /locus_tag="U2M88_001599"
     CDS             complement(13885..14235)
                     /locus_tag="U2M88_001599"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEM8373986.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="U2M88_001600"
     CDS             complement(14365..15876)
                     /locus_tag="U2M88_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEM8373987.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /locus_tag="U2M88_001601"
     CDS             complement(16198..17550)
                     /locus_tag="U2M88_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEM8373988.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="U2M88_001602"
     CDS             complement(17703..18779)
                     /locus_tag="U2M88_001602"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEM8373989.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="U2M88_001603"
     CDS             18975..20720
                     /locus_tag="U2M88_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEM8373990.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="U2M88_001604"
     CDS             complement(21000..21437)
                     /locus_tag="U2M88_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEM8373991.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="U2M88_001605"
     CDS             complement(21421..22794)
                     /locus_tag="U2M88_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8373992.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="U2M88_001606"
     CDS             complement(23116..23604)
                     /locus_tag="U2M88_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8373993.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="U2M88_001607"
     CDS             complement(23626..24228)
                     /locus_tag="U2M88_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEM8373994.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6958 a         5163 c         4489 g         7661 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agctcaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgtc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcgt aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctg caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa gtttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa gcttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc atggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctcc t
//
LOCUS       DASHRQ010000035        23819 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.035, whole genome shotgun sequence.
ACCESSION   DASHRQ010000035 DASHRQ010000000
VERSION     DASHRQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.035"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(45..779)
                     /locus_tag="U2M88_001608"
     CDS             complement(45..779)
                     /locus_tag="U2M88_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEM8373995.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="U2M88_001609"
     CDS             1106..1516
                     /locus_tag="U2M88_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEM8373996.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1509..2210
                     /locus_tag="U2M88_001610"
     CDS             1509..2210
                     /locus_tag="U2M88_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEM8373997.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2210..3796
                     /locus_tag="U2M88_001611"
     CDS             2210..3796
                     /locus_tag="U2M88_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8373998.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3815..4036
                     /locus_tag="U2M88_001612"
     CDS             3815..4036
                     /locus_tag="U2M88_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEM8373999.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4033..4794
                     /locus_tag="U2M88_001613"
     CDS             4033..4794
                     /locus_tag="U2M88_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEM8374000.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5174..5683
                     /locus_tag="U2M88_001614"
     CDS             5174..5683
                     /locus_tag="U2M88_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEM8374001.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5758..6297)
                     /locus_tag="U2M88_001615"
     CDS             complement(5758..6297)
                     /locus_tag="U2M88_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEM8374002.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="U2M88_001616"
     CDS             complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="U2M88_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEM8374003.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7310..8161
                     /locus_tag="U2M88_001617"
     CDS             7310..8161
                     /locus_tag="U2M88_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEM8374004.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8189..8824
                     /locus_tag="U2M88_001618"
     CDS             8189..8824
                     /locus_tag="U2M88_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEM8374005.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8844..9617
                     /locus_tag="U2M88_001619"
     CDS             8844..9617
                     /locus_tag="U2M88_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM8374006.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10025..10822
                     /locus_tag="U2M88_001620"
     CDS             10025..10822
                     /locus_tag="U2M88_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEM8374007.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10800..11213
                     /locus_tag="U2M88_001621"
     CDS             10800..11213
                     /locus_tag="U2M88_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEM8374008.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11197..13842
                     /gene="mutS"
                     /locus_tag="U2M88_001622"
     CDS             11197..13842
                     /gene="mutS"
                     /locus_tag="U2M88_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEM8374009.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13860..15968
                     /gene="mutL"
                     /locus_tag="U2M88_001623"
     CDS             13860..15968
                     /gene="mutL"
                     /locus_tag="U2M88_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEM8374010.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15990..16550
                     /gene="maf"
                     /locus_tag="U2M88_001624"
     CDS             15990..16550
                     /gene="maf"
                     /locus_tag="U2M88_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEM8374011.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16635..18623)
                     /gene="treP"
                     /locus_tag="U2M88_001625"
     CDS             complement(16635..18623)
                     /gene="treP"
                     /locus_tag="U2M88_001625"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEM8374012.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18846..19559
                     /gene="treR"
                     /locus_tag="U2M88_001626"
     CDS             18846..19559
                     /gene="treR"
                     /locus_tag="U2M88_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEM8374013.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19636..20082
                     /gene="msrB"
                     /locus_tag="U2M88_001627"
     CDS             19636..20082
                     /gene="msrB"
                     /locus_tag="U2M88_001627"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEM8374014.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20252..20854
                     /gene="ruvA"
                     /locus_tag="U2M88_001628"
     CDS             20252..20854
                     /gene="ruvA"
                     /locus_tag="U2M88_001628"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEM8374015.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20867..21868
                     /gene="ruvB"
                     /locus_tag="U2M88_001629"
     CDS             20867..21868
                     /gene="ruvB"
                     /locus_tag="U2M88_001629"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEM8374016.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21897..22475
                     /locus_tag="U2M88_001630"
     CDS             21897..22475
                     /locus_tag="U2M88_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM8374017.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22527..23009
                     /locus_tag="U2M88_001631"
     CDS             22527..23009
                     /locus_tag="U2M88_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEM8374018.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23126..23500
                     /locus_tag="U2M88_001632"
     CDS             23126..23500
                     /locus_tag="U2M88_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374019.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7774 a         4284 c         5115 g         6646 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg aaatggagga
     1501 gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg actttaggag
     1561 tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg gcacttgcca
     1741 tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta atggctgcga
     1801 ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac ttcttctctc
     1861 ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct atttctgtcg
     1921 atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt gtctctacag
     1981 gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc gaaagtccag
     2041 ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca aaagcaatcg
     2101 agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac ggaatattga
     2161 ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga tgcgaaaacc
     2221 actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga aagacgtgta
     2281 tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga ttctcctcct
     2341 agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag ttgttcagct
     2401 ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc agcagtttag
     2461 cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact tttatgatac
     2521 gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta cgctgaaatt
     2581 ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat ttgcgcaaaa
     2641 gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac agcaactgca
     2701 aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag aagaaatcat
     2761 tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct ggccatttga
     2821 aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt tttttaatgt
     2881 tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct tttttttaag
     2941 aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga tttctgacaa
     3001 tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt attttcaagt
     3061 agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat attatagtga
     3121 tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac cgccctttac
     3181 agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg ggctgccagc
     3241 agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt tcgcctatct
     3301 ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta atcatttagt
     3361 tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta gacacaaaca
     3421 tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc tgactgtgtt
     3481 taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt taatgacaga
     3541 tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt tccgtaatga
     3601 gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc ttctgatttc
     3661 gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg tgactgaaga
     3721 actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc aaaaacaaaa
     3781 actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa ttttaccaga
     3841 attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac tttatgttgt
     3901 tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat tgatcaaaac
     3961 aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag cagatgattg
     4021 ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt atttatcaca
     4081 ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca agatgctttt
     4141 gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg atttcctgaa
     4201 acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca agcggtatcg
     4261 aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg gatattggat
     4321 gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta tgagacgaat
     4381 gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg ccaaaaaagc
     4441 ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg tgggatcgct
     4501 tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct ggattacgca
     4561 ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc aatgaatgcg
     4621 gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac accagtcaag
     4681 cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc tcctcaagca
     4741 agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa ataaccttgt
     4801 gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg atgataaaaa
     4861 aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa ttctttttaa
     4921 taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa acgatattca
     4981 aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag ctatttttat
     5041 ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc gacacttcag
     5101 ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt atcttgcaaa
     5161 ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga caacgacaga
     5221 tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg ttttgtctgt
     5281 ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc tgtccttgta
     5341 taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata ttgcttctcc
     5401 tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat tgcttatcaa
     5461 ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga cagcatgcgt
     5521 atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc aactaccttt
     5581 tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg tcggtgggat
     5641 aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg tttaatttaa
     5701 aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct tttttgatta
     5761 atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat aagctgctcc
     5821 agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa atgcagttgt
     5881 aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg cagcaagaga
     5941 gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt cgatcatcca
     6001 accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg gcccaaataa
     6061 aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc caagtaaaag
     6121 ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg ttaatccaat
     6181 agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct gagaaaggac
     6241 agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc ggttcataac
     6301 gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa acaaagtgat
     6361 tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc ggtctttttc
     6421 aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga gcttcgtcga
     6481 tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc aatttttcag
     6541 cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct tttactgtta
     6601 cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg tggtatgaag
     6661 ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat tgacgtgctt
     6721 gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt tgttctagta
     6781 agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt acgaacaagt
     6841 tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt tcagttaatt
     6901 gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct ggtgcgtaag
     6961 ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga cgagaaattt
     7021 tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat aagtttttgg
     7081 agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat cttcaccttt
     7141 gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat gagttattat
     7201 ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca cgaatgtcaa
     7261 gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa tgagagggta
     7321 tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc cggtatcacc
     7381 attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg aaaacagctt
     7441 ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag gaacggctgt
     7501 ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa agattttata
     7561 cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg aattaggaac
     7621 atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt taagtaaggc
     7681 agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat tatgtggatt
     7741 tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta tgttagaatc
     7801 aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga tgtttgcgaa
     7861 tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg tcggaaaagt
     7921 gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac acgtgatagc
     7981 gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg atggaatgac
     8041 agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata ttggtggcgg
     8101 tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct atgtggacta
     8161 aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa aaaatgagga
     8221 aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg gagtacctaa
     8281 gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt tatctatttc
     8341 tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt atcagtatgt
     8401 agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact tagtcgtatt
     8461 gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga gaaaagtata
     8521 cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat tgtttgaaaa
     8581 aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt ataaacagca
     8641 aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa aggaattctt
     8701 tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg ccgattgggt
     8761 aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa gtaagtattt
     8821 ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat attgatggaa
     8881 cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg gaaaaattac
     8941 gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat gcgcaagaga
     9001 tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc ggttttttag
     9061 accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg ttcgttcaag
     9121 aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc aagcgttctt
     9181 cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct gtcttgcctg
     9241 aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca tttttcgatc
     9301 aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc tggcatgaga
     9361 acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg cacttagcag
     9421 aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa aacgaccgtg
     9481 aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca gaagtacaag
     9541 cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag gcattgaaag
     9601 agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt aataagccca
     9661 gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc gcagatgcaa
     9721 gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc aagaaataag
     9781 aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat ttctgaagaa
     9841 gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg tcatagtccc
     9901 atttttttat tttatttttc aaaactaaga aataccagga aagagttcct tttgtttgac
     9961 cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag aagaggaatt
    10021 tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag agatgattac
    10081 tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg taaatggtga
    10141 gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt tgcaagacgg
    10201 cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct ttgagtttgt
    10261 aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc ctggacaagg
    10321 gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc aggctcgttc
    10381 ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag aagaagccag
    10441 aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg aaaaacaagc
    10501 aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata cacacgttca
    10561 gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg ttgggatgac
    10621 aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa agtttctgac
    10681 tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa gcggctgtat
    10741 cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc agatcaacga
    10801 tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag ccggaaattc
    10861 aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc attcgctaca
    10921 aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga gaaaaaatca
    10981 aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca gcagaaaaag
    11041 aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg cttgttgtag
    11101 cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca actatgatac
    11161 aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac taaaaatact
    11221 cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt tttgttctat
    11281 cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc acagcttttg
    11341 gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat gtgcggtgta
    11401 cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta taaagtagcg
    11461 atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg cgaagtgatc
    11521 cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa agacaacaat
    11581 tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc cgatttaagt
    11641 acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa tgaagcctct
    11701 gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct tcgtgaacag
    11761 ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga aaacgcggaa
    11821 tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac cggcaaacta
    11881 ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa agcagtagaa
    11941 taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct agaattaagc
    12001 caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga tgaaacaaaa
    12061 acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat ccaggcaaaa
    12121 caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt tgaacgtatc
    12181 gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg tcgagtagct
    12241 ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga acaagtgcca
    12301 atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct gctttcagaa
    12361 atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga tgatccgccg
    12421 ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct cgatatgtac
    12481 cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa agagcgtcaa
    12541 gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta ttatatagaa
    12601 attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa acaaacttta
    12661 gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca aatcctcgaa
    12721 gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag agaagaagtc
    12781 aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt agatgtgcta
    12841 caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact tgtcagtgac
    12901 aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt attaggacat
    12961 caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt gctgattaca
    13021 ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac tgttctgatg
    13081 gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt tgatcggatt
    13141 tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt tatggtagag
    13201 atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat tttgtttgac
    13261 gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc aatcatcgaa
    13321 tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca tgaactgacg
    13381 gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt tgaaaaagac
    13441 ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag ctacggcata
    13501 catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc agatatcctg
    13561 catacgctag aaaatggcga taacggacac catactgttc cagctgaagt agaagagaaa
    13621 actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa tcaagaccat
    13681 atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga attatccgaa
    13741 aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat gacgccaatg
    13801 gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg cggatatcga
    13861 tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg ggagaagtag
    13921 tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac gccggcagta
    13981 ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg attgataacg
    14041 gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg acaagtaaga
    14101 tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg gaagctttgc
    14161 caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa gaaaaacaag
    14221 gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca ccactgcgtc
    14281 aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc ttgaaatacg
    14341 tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga ctggcattga
    14401 gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg gcaactgctg
    14461 gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc gcaaaaaaaa
    14521 tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt tcattgcctg
    14581 aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt tttatcaaga
    14641 acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg gtcggtcgtt
    14701 tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta aatgttcatc
    14761 cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta attagtaacg
    14821 ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat aatctacggt
    14881 ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat ttgtcctacg
    14941 cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga gagacaggta
    15001 cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat aagtctgtgg
    15061 aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca gaaaaaacag
    15121 cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa acagtggaaa
    15181 aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat gaagaacaag
    15241 cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca ctccatcctg
    15301 aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag aaattagaaa
    15361 cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg cacggaacgt
    15421 atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct gcccaagagc
    15481 gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat ctgcaagaat
    15541 tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta aaagagcaaa
    15601 aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac agctttattg
    15661 tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt cgggagatga
    15721 tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa gcaacagcaa
    15781 tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa gcacaagcac
    15841 gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc catggccgtc
    15901 cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga atccaagacc
    15961 cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc caatcaccaa
    16021 gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct gctgatatcg
    16081 atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg gctgaagaaa
    16141 aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt gccagtgaca
    16201 cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct gaggctgaat
    16261 tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt gttttaaaaa
    16321 caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat cctttgacag
    16381 acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca ggggcatacg
    16441 gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat tacagtatcg
    16501 taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa acaaaaaagc
    16561 ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt gaaaagatta
    16621 cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc cgggcgaaca
    16681 agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa agcaacaata
    16741 atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc aatcagggtc
    16801 tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc ctttagttca
    16861 acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag aataccaatg
    16921 gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata gactttgccg
    16981 tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac tgggtcattt
    17041 acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc ttttgttgca
    17101 actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt tgcgccattt
    17161 gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat cttattcaaa
    17221 attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc aatgatcatt
    17281 gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg caaaccgcct
    17341 actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc taatccagag
    17401 ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa catagcaggt
    17461 tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc ttctttttta
    17521 ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat atttgataaa
    17581 gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat atcgatggca
    17641 ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc gaatacggca
    17701 ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga gatccagctt
    17761 ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt tggtaacaat
    17821 gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg gaagaagatt
    17881 tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc ttgataacca
    17941 atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc ggctgtggaa
    18001 gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa aacgattcct
    18061 aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac tggaagaaaa
    18121 tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca aaacttcgag
    18181 tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac atttctgaat
    18241 ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat ttctgctaaa
    18301 acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt tccttgttct
    18361 ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt agaaacatcg
    18421 tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac gctaggtata
    18481 tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg catacgagtc
    18541 gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa cagcttctcg
    18601 gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg tctatgcaag
    18661 ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt attaaaaagt
    18721 acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa aaataggaac
    18781 attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg agtgagggaa
    18841 aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt ttagcgaaag
    18901 aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt tatggtgttt
    18961 cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat attcaaaaaa
    19021 aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct atttctggat
    19081 tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg atcatccata
    19141 cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg gagatcggtg
    19201 cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt atattagaca
    19261 ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca caaggatcga
    19321 tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa aaggagatca
    19381 cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat gacacttatg
    19441 cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag tattctgagt
    19501 cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga aaagtgtaaa
    19561 tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga taggatcaca
    19621 aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact gaaagaaaag
    19681 ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc atttagcggg
    19741 aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg tgaaccgtta
    19801 ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag caaaccaata
    19861 acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg aatagaagtt
    19921 cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc agtagatcaa
    19981 ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata tgaaaagatg
    20041 gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata cacaaagagc
    20101 ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg attgctattt
    20161 gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca ataaagggaa
    20221 cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag gtgtggttac
    20281 ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc agatcgcact
    20341 aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt atgtccatca
    20401 ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag aaaagcaact
    20461 gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg caatcatggc
    20521 aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca cttatttgac
    20581 gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc taaaaggaaa
    20641 attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa acacaaaaga
    20701 cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca gtgatcgaga
    20761 aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg atgagtatct
    20821 acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga cagatgaata
    20881 cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc ttcgaccaca
    20941 gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga tttacatcga
    21001 agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc ctcctggatt
    21061 aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata ttcgtacgac
    21121 gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg aactggaagc
    21181 aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag aagagatgct
    21241 ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc caaccgcaca
    21301 tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac gggcaggtat
    21361 gctttctgca cctttacgag atcgatttgg catcatttct catatggaat attatgacga
    21421 acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag agatcattga
    21481 agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg ctaatcgctt
    21541 gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg atcgtcctat
    21601 agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt atgttgacca
    21661 gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt taagtacgct
    21721 ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac cgtatctgat
    21781 ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt atgcctatga
    21841 acattttggt tacgactatc atatatgatt tttgctttta gattggagga aaatgaatga
    21901 gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg tgccaaacgc
    21961 aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc aatcgacaaa
    22021 cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat tgtgaggatg
    22081 cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag caagcattga
    22141 aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg ctttctgacc
    22201 cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc aagatttttg
    22261 aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat gctcgttttt
    22321 tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat gaagaaacac
    22381 cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta tattcttatc
    22441 gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg ttggaaaagc
    22501 aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt ttgggaaata
    22561 tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta gaaggaaggg
    22621 aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga aagacaccgc
    22681 accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat atggttgctc
    22741 ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg gatcaagaaa
    22801 atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc catttgtttt
    22861 taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat accaataact
    22921 ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat atttggattg
    22981 atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag gcgatgttag
    23041 agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa gtaacacttt
    23101 cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa tgaagaagct
    23161 tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa atgacactga
    23221 agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc aaaaagctgc
    23281 tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta gcaagtatga
    23341 aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga tgatccgcct
    23401 acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa aaataacaat
    23461 cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag atagaaaagc
    23521 acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc tttaagctga
    23581 tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt cggttgatta
    23641 gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct agccggggag
    23701 ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt tggtatgctt
    23761 atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt tgttaagtt
//
LOCUS       DASHRQ010000036        23659 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.036, whole genome shotgun sequence.
ACCESSION   DASHRQ010000036 DASHRQ010000000
VERSION     DASHRQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.036"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            257..532
                     /locus_tag="U2M88_001633"
     CDS             257..532
                     /locus_tag="U2M88_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374020.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2623
                     /locus_tag="U2M88_001634"
     CDS             545..2623
                     /locus_tag="U2M88_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEM8374021.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2783..4669
                     /locus_tag="U2M88_001635"
     CDS             2783..4669
                     /locus_tag="U2M88_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEM8374022.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4681..5628
                     /locus_tag="U2M88_001636"
     CDS             4681..5628
                     /locus_tag="U2M88_001636"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEM8374023.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5648..6175
                     /locus_tag="U2M88_001637"
     CDS             5648..6175
                     /locus_tag="U2M88_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEM8374024.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6238..6891)
                     /locus_tag="U2M88_001638"
     CDS             complement(6238..6891)
                     /locus_tag="U2M88_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEM8374025.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7023..7865
                     /locus_tag="U2M88_001639"
     CDS             7023..7865
                     /locus_tag="U2M88_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEM8374026.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8023..8919
                     /locus_tag="U2M88_001640"
     CDS             8023..8919
                     /locus_tag="U2M88_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEM8374027.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8922..9635
                     /locus_tag="U2M88_001641"
     CDS             8922..9635
                     /locus_tag="U2M88_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEM8374028.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9655..10173
                     /gene="msrA"
                     /locus_tag="U2M88_001642"
     CDS             9655..10173
                     /gene="msrA"
                     /locus_tag="U2M88_001642"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEM8374029.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10170..10397
                     /locus_tag="U2M88_001643"
     CDS             10170..10397
                     /locus_tag="U2M88_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEM8374030.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10549..11100
                     /gene="lepB"
                     /locus_tag="U2M88_001644"
     CDS             10549..11100
                     /gene="lepB"
                     /locus_tag="U2M88_001644"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEM8374031.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11183..11683)
                     /locus_tag="U2M88_001645"
     CDS             complement(11183..11683)
                     /locus_tag="U2M88_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEM8374032.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11962..12360)
                     /locus_tag="U2M88_001646"
     CDS             complement(11962..12360)
                     /locus_tag="U2M88_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEM8374033.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12620..13480
                     /gene="ylqF"
                     /locus_tag="U2M88_001647"
     CDS             12620..13480
                     /gene="ylqF"
                     /locus_tag="U2M88_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEM8374034.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13473..14240
                     /locus_tag="U2M88_001648"
     CDS             13473..14240
                     /locus_tag="U2M88_001648"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEM8374035.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14296..15153
                     /gene="dprA"
                     /locus_tag="U2M88_001649"
     CDS             14296..15153
                     /gene="dprA"
                     /locus_tag="U2M88_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEM8374036.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15275..17353
                     /gene="topA"
                     /locus_tag="U2M88_001650"
     CDS             15275..17353
                     /gene="topA"
                     /locus_tag="U2M88_001650"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEM8374037.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17322..18698
                     /gene="trmFO"
                     /locus_tag="U2M88_001651"
     CDS             17322..18698
                     /gene="trmFO"
                     /locus_tag="U2M88_001651"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEM8374038.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18896..19801
                     /gene="xerC"
                     /locus_tag="U2M88_001652"
     CDS             18896..19801
                     /gene="xerC"
                     /locus_tag="U2M88_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEM8374039.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19838..20386
                     /gene="hslV"
                     /locus_tag="U2M88_001653"
     CDS             19838..20386
                     /gene="hslV"
                     /locus_tag="U2M88_001653"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEM8374040.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20400..21800
                     /gene="hslU"
                     /locus_tag="U2M88_001654"
     CDS             20400..21800
                     /gene="hslU"
                     /locus_tag="U2M88_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEM8374041.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21818..22609
                     /gene="codY"
                     /locus_tag="U2M88_001655"
     CDS             21818..22609
                     /gene="codY"
                     /locus_tag="U2M88_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEM8374042.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22691..23566
                     /locus_tag="U2M88_001656"
     CDS             22691..23566
                     /locus_tag="U2M88_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEM8374043.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8192 a         4079 c         4983 g         6405 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaaacgag tccaataaaa taaaaaggta aaaattttca
     2641 agttttataa aaaacaaaac atatcagaac aggatcgaat cggtcctgtt ctttttttga
     2701 gaacacaaaa agaacaaata ccaaaaaaat tttcctgaag ttcactattt ttatgataca
     2761 atatccaaga taaggaggga acatgttgaa agaattaaaa gtaactgacc tcaaaaaaac
     2821 ttatggtgaa aaagatttgt ttgaccagat ttcttttttg gtccacgaaa aagaccgtat
     2881 cgggttgatt gggacgaacg ggacgggtaa aacaagtctc ttaaatatta ttgccggtat
     2941 cgatagcagc gacggtgatc ggcaaacagt gttttatcca actgattacc gtattggtta
     3001 tctttctcaa gcagctgaat tttccgatga attaactgtt ctccaagcag tctttcaagg
     3061 aaacagcccc ttgatccaaa ctgttcgagc atatgaggaa gcattgatcg aactagggga
     3121 aaacggagaa gaccctgatg tgcaaaaacg ttatgctaaa gcagaagaac aaatgaataa
     3181 agaagatgct tggactacag ataccaacgc aaagattatt ttgcaaaaat taggaatcag
     3241 tgaattagat aaaaaaatca gtactttatc aggtggtcag aaaaaacgag ttagtctggc
     3301 gcaagtgttg attgatgaac ccgatttact attactagac gaaccaacaa accatttaga
     3361 ttatgaagcg atcgagtggt tggaaaacta tttgaaacaa taccgtggcg cactattgat
     3421 ggttacccac gatcgttatt ttctagatag agtagcaaac cgaatcttcg aactttcatt
     3481 tggaaaatta tacgagtata aaggaaatta tgaagcttac gtcttagaaa aagcagaacg
     3541 tgatcgtgta gaagtagaac aagaagaaaa gcgcaagcgg ctttataaac aggaattagc
     3601 ttggatgagg gctggcgtac aggcacgtgg caccaagcag caagcacgga tcaaccgttt
     3661 cgaagatcta aaggagaact tgtaccaagt aaaccaagaa gatgatttag agctgaacct
     3721 agcgacacaa cgtttaggga aaaaagtact ggaaatcaaa gatggcagtt accgtataaa
     3781 tgaccaaaca ctgctagaac atttagattt attgattcaa tctcgggaaa gactaggtat
     3841 tactggtaaa aatggtgcgg gaaaatcgac attgcttaat attctagctg gcagaattcc
     3901 cctggatagc ggtacaatgt ctatcggtga aacggttcat cttgcgtatt atacacaaga
     3961 aaatgaagaa atggcaccag ataaacggat gattgcttac ttacaagaag ctgctgaaga
     4021 agcaaaaaca gcagatggca gtcaaatcgg agtagcagaa ttacttgaac gatttttatt
     4081 tccaagattt atgcatggga cactgatagg caaactttca ggcggagaaa aacgccggtt
     4141 atttttgttg aaactgctga ttcagcaacc taatgtttta ttattggatg aaccgaccaa
     4201 tgatttggac atagcaacat tgacgatttt ggaagattat tttagaagtt ttccaggtgc
     4261 tgtgatcact gtttcccatg accgttattt cttggacaag gtagcagata agttattagt
     4321 attccaagga aacggaaaac aagaacttta ttacggaaac atgtccagtt atttactgaa
     4381 gcaaaaagag acccagcagc ctgctgaaaa agcgaaacca aaaatacaga gcaaggaacc
     4441 tgctgaaaag aagaaattat cttacatgga acaaaaagaa tgggaaacga ttgaagatga
     4501 gattgcagaa cttgaagaaa aaatctcatt gctccaagaa gagatgaatc atcaaggaga
     4561 taatttcact cgattacaag aattgcaaaa cgacgtatct gaaacagaag cacaacttga
     4621 agaaaaaatg gcacggtggg aatacttgag tgaatgggta gaagattagg aggaaaaagg
     4681 atggaacaag cgtatcttga cttaggaaga aaattgttgg aagaagggca tcgtaaagaa
     4741 gacagaacag gcacaggcac attaagctta ttcggatacc aaatgcggtt tgatctgcaa
     4801 aaagggtttc ctttgttaac aacgaaacga gtaccttttg gattgatcaa aagcgaattg
     4861 ctttggtttt taaaaggaga tacgaatatc cgctatctat tacaaaataa aaaccatatt
     4921 tgggatgaat gggcatttga acgctacatc aaaagtccag actataatgg accagatatg
     4981 actgattttg gccatagatg tttgacagat cctgaattca atgaagaata taaaaagcag
     5041 agcaagcttt tttgcgagaa gatcctaacc gatgattcct ttgctgaaac atacggcgat
     5101 cttggacaca tctacggcta tcaatggcgt cactgggaaa caaaagacgg tggctttatc
     5161 gatcaaatca aacaagtgat cgaagcaatc aaaaagactc ctgattctag aagattgatc
     5221 gtttctgcat ggaatccgga agatgttcct agcatggctt tgcctccttg tcatacgatg
     5281 tttcaatttt atgttcaaga gggacggcta agctgccaat tgtatcaacg cagtggggat
     5341 gtgtttctag gcgttccgtt caacattgca agttatgcgt tgctgactca cctaattgca
     5401 catgaaacag gtttagcggt tggcgaattt gttcacacat taggagatgc tcatctttat
     5461 atcaatcata tcgaacaaat gaaagaacaa ttgagccgag aaatacgcgc atttccgact
     5521 ttagttttga atcaggaaaa gcagtcagta tttgattttg agatggaaga tatccaatta
     5581 gaaggatatg acccacatcc ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga
     5641 tgagaaaatg ttagctgcaa tttgggcaca ggatgaaaac ggattgattg gaaaagaaga
     5701 ccgtcttcct tggcgtttgc caaatgatct gaaattcttc aaacaaatga cagaagcaaa
     5761 tacactagtg atgggacgaa aaacattcga agggatgggt agccgtccgt tgccgaatcg
     5821 tcagacgatt gttttgacga gagattcttc ctataaagca gaaggcgtgc atgtcatgca
     5881 tagtgtggaa gacgtgcttg ctctggaaaa agaaacagac ggtatctttt tcatcgctgg
     5941 agggtcagct gtttatcaag aatttctgcc attttgtacg atattatacc gtacagtcat
     6001 ccatcatgta ttcgatggag atgcttattt tccacctgtt gactggtctg attggtcact
     6061 gatcaatact agtcaaggag aaatggatga aaaaaatcgt tatccgcatc aatttgaaac
     6121 gtatcagcgt aatgagaatc ctgtaaaaag cgggacaggc gaattgacga tataaaaaag
     6181 ctatggatag actagctgtt atttagagct gtcccatcca tagctttatt ttttttatta
     6241 agtaaagaat aaaacagaga agtacataaa acctgcacct aacataacaa acaaatgcca
     6301 aacaacatgc atatagcgga tagaattcaa gctataaaag aatgcgccta tcgtgtaaga
     6361 aagcccgcct gctaccatca aaataacacc agtcattcca agagagtgat aaagaggaac
     6421 agcagccacg acacagagcc accccataaa aatatagatg atcgtagata cttttgatac
     6481 tctctcccgt ttatgcaacg tcaacgcttt atatataatg ccaagaatcg caaacaacca
     6541 gattaataca aataaagtcc aacctagcca tcctcggata ctcagcaaac aaaaaggagt
     6601 ataacttcct gcaatcaaaa gaaaaatcga actatgatcg aatacttgga acaccttttt
     6661 tgcttttgta aaaatcaatg aatgaaacaa agtagacgtt aaaaataaaa gaatcatcat
     6721 cgatccatag atagcataag agacaatatg gagggaggaa tgcaagtggg caccttttac
     6781 taaaagaacc actagtcccg cgatactcaa tccagtccca attccatggg tgactgcatt
     6841 aaacacttca tttaatattt catacgttcg attaaatttc gttttttcca tgaaaacggc
     6901 tcctttacta ggtgtaatta atgaaaaatc attatttgtc tgttatacta aaaatagaaa
     6961 ttgtcttaca tgtagtttaa catgaataag gcatcttgtt aaagaaagag agagagaaaa
     7021 taatgacaaa tgtaaaaatc gtaacagatt cttcatgtac gatggaaaaa aatgtacgcg
     7081 atgctttagg cattcgtgtc atgccgctat ccatcatgat cgacggaaca gtatatcccg
     7141 atgatgacca actagacggc gaaagattta tggaaatgat ggcgaaagca aaaaatctgc
     7201 ccaaaacaag ccaaccacca attggagaat tcatctcgat gtatgatgag ctaggagaag
     7261 acggcagtga agtgatttcg atccatatga caaaaggctt gagtggaaca gtagaagcag
     7321 caagacaagc cagtaatcta acaaaaacga aagtaacggt tatcgacagt gataccactg
     7381 accaaggatt atcttttcag gtaattcgag cagctgaatt agcaaaagaa ggtaaaacag
     7441 tagagaaaat ccttgaagaa gtcaaaaaag tacaagacaa tacaaaatta tacatcggaa
     7501 tatccacact agataacttg gtaaaaggtg gaagaatcag ccgaacaact ggtttgctgt
     7561 ctagtgtttt gaacatacgc gtggtgatga atttcgccca ttctgaatta atccctgtaa
     7621 ctaaggggcg aggaaagaaa acgttcgata aatggtttaa taatttgaaa aatgaactga
     7681 aagaattgcc aaatgtgcga caaatcggta tttcacatgc ggatgcaaaa gaattggcgc
     7741 aagaattcaa agagggtttg caagaattat tcccggacat gcatatccct gtactgcata
     7801 cgaatccggt tatcgctacg catactggta aagacgcgtt tgcaatcatg tattatgtag
     7861 attagtcgaa aagaggataa gccctcagcc tgaaaggctt gcagcttatt ctctttttct
     7921 ttataaaatt aggaaaataa ttgttttctt ttttcataaa gactaccttt ttgataaaat
     7981 ttttgataac attagaacca atcgaataca agaggtgaaa aaatgaaatt aacgaaacga
     8041 catttgatcc tcgttttgtt cccattatta attgcgatca tcatctatgc aggtttagct
     8101 gcttctatac cgaaagctgg cccactaatg aaaccagtaa ctactgtgag agaagatcca
     8161 aataaaaaag aaaaacttca ctacatagct atcggagatt cgctgactga aggagtagga
     8221 gatcaaacca agcaaggagg attcgttccc cttgtcgcaa atgatctaaa agaccgttat
     8281 aatttgaccg cggttgaaac agaaaactat ggtgtaaatg gtgaacgaag tgaccagatc
     8341 ttaaagcgag taaaaacaaa agaagaaatt cgaaacaata tcaaaacagc tgattttatc
     8401 acactgactg ttggcggtaa tgatttgatg aaagttatcc aaaataattt attcgggtta
     8461 acggtcaatt cctttaaaaa gccactgaaa aactatcagg aaaatgtcac agaactattg
     8521 gaagaatttc gatccctcaa taaaaccgca cctatttaca ttttaggcat ctacaatcca
     8581 ttttatttga actttcctga gatcactgac atgcagacaa ttgttaacaa ttggaacaaa
     8641 ggaacagaaa cggtgacaaa aaaaatgccg aactgctact ttatcccaat caatgatttg
     8701 ctttatcaag gattgaatca ggaagtaggt gtcacagaag aaggaacaga tcaaacaact
     8761 agcacgacag aaaatctaaa tggcatcaaa aataatgtgc tctatgaaga agatcatttc
     8821 catccaaata atttaggtta tcaattgatg gcaaatgcag tgagagatca gctgatcgag
     8881 acacaaaacc aatggttaga gaaggaggat gatcaataaa catgaaaaca agatctgacg
     8941 tatcttcaaa caaaacaaag aaaacaaatt actggaaatt cgccttcctt atccttcttg
     9001 gggtagtact tggttcgacc gtatttttag gcagccgtat ttttgctaat cgggaaccag
     9061 aactcccaga aataccagct cttacggaaa gacaaggaga tcctgtctta acgatcaatt
     9121 caaataaaga aaaagtcaat caaatcatca gctttttcct tagtgagtat caaaaagata
     9181 gtgatattga atataaattt tatttggaaa atgaagcact actcaatggt actttcgaag
     9241 tactaggttt tcctatcgat ttctatttat attttgatcc gtatgtgatg gaaaacggaa
     9301 atgtccaact aaaagctaga agtctttcta tcggaacatt gagtttgcca atcaaagatg
     9361 tgatgaatat gatcaagaga aactataaat taccagattg gatcgaagtc aatacagatg
     9421 atctgactat catgcttagg ttggatcaat tccgaatgca aaacgggatg tatattaaag
     9481 cagataaaat cgacttagta aatgatgaca ttcggtttag tctatatttg ccaaaagata
     9541 cctctgatgc ttcaaacaac tcaaatacca acaaaactag caccagcagt actgaaagca
     9601 agacaaaaga aaataaaaaa tcatcggatg agtagtatta gagggggaat cataatggaa
     9661 cgagcaatat ttgcaggagg ctgcttctgg tgtatgatcc agcctttcga cacgcttcct
     9721 ggtatccata cagtgatgtc aggatatact ggagggcatg taccagatcc gacttatgaa
     9781 caagtaaaaa gccaaacgac tggacataca gaagcagtgg agattatcta tgatccaaca
     9841 ttgatttcat atgaacaatt agtagaactt tactggcagc aaactgatcc aactgacgct
     9901 ttcggacaat tcgaggatcg cggtgacaat tatcgcccgg tgatctttta tttgacagaa
     9961 gaacagcgga tcattgctga aaaaagtaag aaacagttag ctgaaagtgg acgtttcgaa
    10021 cagccaatcg ttacaaaaat cgaaccagcc caaccatttt atctagcaga agaagaccat
    10081 caagagtatt ataaaaagca tcctgaaaat tttaaaagaa accatgcacg ccgggctgcc
    10141 tttatcgaag aaaattggag gaacgaaggg tgaatcgaag cttttttcat tatttaatga
    10201 cactgcgagg cggtggcaca catgatgctt tatctgtttt cgctacagaa gccggtaaag
    10261 acatccaatt tcctaagcac agctctagct acgaagaaat ttctaattat ctggaactga
    10321 atgtcgacta tttgccgtcc atggatatct ttgacgaagc atgggaaaaa tatttagaaa
    10381 ataatcatga catataagag atagttttgt actttatgat caaaaaagtt ttttgtccaa
    10441 tagcctaatt gaaagaatta tttttatttc agctatctag tcgcattttt ttgatatttt
    10501 tcttataata aagaaagaat gaaaaaaaga gggatggatt gatagagatt gacaaaaaaa
    10561 caacgctata ttaatcgctt ctggcttgta ttcaaatact tacttgtttc tgtattcctt
    10621 gcgttcatgt tgcggggatt tttattcatc ccggtaccag tggaaggcaa ttcaatggaa
    10681 aacgtgttga agcaaggtga tatggttgtg atggaaaaat tttcggagat cagacgattt
    10741 gatattgttg tttttcaatt agcagatggc acgatttata tcaaacgagt aatcggtctt
    10801 ccaggagaaa atgtcagcta ccaaaatgat caattgaaga tcaatgggaa agtagttaag
    10861 gaaccttatc taactaaaaa cctgaaatca gatcatgcaa atgcatccta tacgacggac
    10921 tttacactac aagagctaac gggacaatcc aagcttccgg aagataatta ttttgtactt
    10981 ggagataatc gaagagtttc aaaagatagt cgttcgtttg gcacgatcaa taaaactgat
    11041 attctaggta aagcaagatt tgtctattat ccacttgatg aaataaaatg gatccagtga
    11101 gaaacaataa agcgtgcagt ctgttgagac tgcacgcttt attgtttaat ccaaaatcag
    11161 tttactacct tagtacgtaa aatcaaacag ctcgtttaaa cacttttcta gcctctaaag
    11221 ttttgataaa tgaataaaca accattattt gaatagggat ttggatcata ttcctgatga
    11281 atctacctag aaaaattact ttgaaatctg caccaaacaa aacagacatc agccaagaat
    11341 tgataaaaag gctgactggg atcataatca ataattgggc agcgatgacg gaaggcaggt
    11401 ttatcttttt atgatataaa aataagccat taataaatcc accaaggaaa gccgtgataa
    11461 gaaaagcagc ttgaaacgtt cctgaaccaa atatcgtgaa atttaaagtg ttcgacaaca
    11521 tttcaagcaa accagcccag atcggtccat atagataacc cataagcgcc atacttaaaa
    11581 atccgaatgt gatttgaaaa taagcaggac caatcgctat cttgtataag atgacatcaa
    11641 gggctaataa aagagcagag gtcgacaaca atcttgcatt catttcgggc aatctaaaaa
    11701 cgtcttttgc aatcaaagtg ttcaaaatat tctctccttt gtcagttaat aataaaagga
    11761 taccataaaa tcaccaatgt gtcatgacac attggtgatt tacatacact ggcatctaac
    11821 aattaaaaaa agggtgagaa gactgctttt ataaactgta aaaaagagat tgtacgaata
    11881 aaaaaaatag aggcggagaa tacttatcgt atcatccgcc tttgtttttt tgttataaaa
    11941 aacgtcagat tagttatctg cttacatctt catccatacg ggttctaatg tatttccgtc
    12001 tagatcttgg acttctaatg aaaacatttc ttcttcaggc attcccatat ctacttcgtg
    12061 gaaagtacca ccattttctt ttgcaatttg agcaaatttt tttacttcat ccgcattttc
    12121 aacactaaac gaaatcaaag caccacttgt ttttttatta tcagctattt ctttatcttt
    12181 tataaatttt ttataaaaat catgagagag aagcattatc caaaaagtgt catcccatac
    12241 cattgaacca gcttcttcag tagaaaattc ttcatttttt ttaaaaccca gcttttcata
    12301 aaaagcaacg gaacgattga catcagcaac aggaaaatta acaaaaacca tactattcat
    12361 ttgtttcgtc tcctttctgt tttgttcgtc tatcatctct tatttaggaa acgctgtcaa
    12421 aaatttagat tcatatatag aaaccaaaat aaaaatttgc atcatcaaaa aagtgaagat
    12481 gtgatagaat gaggactggt tcactttcac ttaactaata gatggaattt ctttataata
    12541 aaaaagtgaa tttatattga aaaagaaaaa aacaagtaaa gccggggaat attccggtat
    12601 gaaaggaatg gagagaacaa tgaccatcca atggtttccg ggacatatgg caaaagcccg
    12661 acgagaagtt tctgaaaaaa tgaaatatgt ggacatcgta tttgaattgg tagacgcacg
    12721 attaccttta tcttctcgta atccaatgct agatcagatc attcagcaaa aacctcgcct
    12781 tgttttattg aataaagcgg atctggcaga tccacaacag acaaagctgt ggcaagaata
    12841 ttttcgtaaa caaggccatc ttgcattagc tatcacggca caagaaagta aaggaattaa
    12901 aggtctagta cctgcggcta aagaagcatt gaaagaaaaa cgggcaagag atgctgctcg
    12961 ggggatcaag cctcgcgcta ttcgggcaat ggtcttaggt attccaaatg ttgggaaatc
    13021 tacgttgatg aatcgactag taggcaagaa aattgcacag acaggaaata agcctggcgt
    13081 aacaaaaggt cagcagtggc ttcgtttagg taatgagttg gaattgttag acacaccagg
    13141 gattttatgg cccaaatttg aagatgaaga aatcggcaaa aaactagcgc tgacaggcgc
    13201 gatcaaagac cagcttgtcc atttagatga tttagcgatt tacggattag acttttttgc
    13261 ccgatattat tcaggaagaa tcagcgatcg ttatggttta tcaatcgagc aagaagaaaa
    13321 attggcaccg gaattactga tggatattac agcaaaacgt gggtataaag aagactatga
    13381 tcgtggcagc gaattagtcg tgcatgatat tcgtcaagga aaacttggac gatacacact
    13441 ggatcgttgc gaagaaatgg agcaaaaaag tgatgactga atccatccaa agtatcaaag
    13501 aacgattgaa aagcatacag agaatagatg atcctacact agaatcatta agaaatgatc
    13561 cacgcaaagg cgtgcagcaa gcactggctt cttttgaacg acgattgaaa aaggaagcat
    13621 taattaagga aaaatatgaa gaaatgtttt gctttgaaaa gcaagggtac gcacaaggtc
    13681 accaatggat tgccggcatt gatgaagtag gaagaggacc attagctgga cctgtcgtgg
    13741 cagctgcagt tatcttgccg aaaaatgaga tcatattagg gctgaatgac tccaaacaat
    13801 tatcagagaa aaagcgagaa cttctttatc aacaagttca agaaaaagcc ctggcaatcg
    13861 ggatcgggat cgttgatcag cagacaatcg atgatgtgaa tatttatgaa gcaagtaaat
    13921 tagcgatgat ccaagcagta gaaaaattgt ccattgtacc aagctatttg ttgatcgatg
    13981 cgatgacctt ggatttgcct attcagcagg aaaaaataat caaaggggat gcacgatctg
    14041 tgtcgattgc tgctgcaagt atcattgcta aagtatatcg agaccaattg atgaaagaat
    14101 atgacgtgct ttatcctgga tatggtttct cgcataatgc aggttatgga acaaaggaac
    14161 acttagaagg acttgaacga caaggaatta cgccaatcca tcgtaaaagt tttgcacctg
    14221 taaaaaaata tttacaataa aaaacagccg cttttacgta tcttattaga aagagagacg
    14281 aaaggcggtt ttatgatgta tcaaatagaa gaaaatttat tgaaacttgc ttatacaaaa
    14341 ggaatcagta gtcaaggaaa atgggcactt gctaactgga tgatgcagta tccttataaa
    14401 gaaatcggat ttgaagacat cattaagata ggtaaaataa ccaaacaccg ggagctgttt
    14461 cgcaaaagtt ggcaggaaat tcacgctaac tggggcaaaa tccaatcgaa acagtcgttt
    14521 atcacttgct ttgatcccaa atatccccct cagctgcttc acttgaccta tccgccaatt
    14581 gtactgtttt ataatggtga tctatcttta ctttcttgta acatgttaag cgttgttggt
    14641 ggaagacaca cttctgcttt agccaaaaaa acagtttatt atttactaaa accagtgata
    14701 gaagctggat atgtgatcgt cagtggctgc gcgaaaggga tagatacata cggtcaccaa
    14761 gcagcaatct ctcatggtgg acggacaatc gcagtcattg gaacagggat agagcaagtc
    14821 tatcctgctg aaaatcgtga cttgcaagag gtgattgcaa atgatcatct tcttttgagt
    14881 gaatatcctc ctcaatccgg cccgaaaaag cattgttttc caatgagaaa tcggattatc
    14941 gcagctttgt ctaaaggaac atgtgtcttt gaagccaaga aaaaaagcgg ctctctgata
    15001 acagcacagc aagcattgga tcttggaaga acagtttttt ctgttccagg aaatatttta
    15061 acagaacatt ctaccggatg tcatcatttg atccaagacg gtgcagtttg cacggtttcg
    15121 ggtgaagata tcttggcaga atttaaagaa taacaccata cttttcattt gcttttcttg
    15181 acaaacgttt tctgaatgga tatgattagc tacgatttgt atgaatcctc ttttaatagt
    15241 aggaacacga caatggaaag gagtccattc agtaatggcg tataaatatt tagtgattgt
    15301 agaatcacct gcaaaagcca agacaattga gaaatatctt ggacgaaact ataaagtaat
    15361 ggccagtgtt ggccatataa gagatttacc gaaaagtaaa atgggtatcg attttgaaaa
    15421 caattatgaa ccccattata tttctatacg cggaaaaggc gatgtcatca aaagcctgaa
    15481 agccgcagca aaaaaagctc aaaaagttta cttggcaagt gacccggata gagaaggaga
    15541 agcgattgct tggcatttag cgtacctact tgggttggat ctgaaagaaa aaaatcgggt
    15601 ggtcttcaat gaaatcacaa aagacgcagt caaagcagct tttaaggaac caagaacgat
    15661 cgatgtagat ttagtagatg cacagcaagc tcgtcgtacc ttagacagaa tcgttggtta
    15721 ttcgatcagt cctattctct ggcgtaaggt caagaaaggg ttaagtgcag gacgtgtcca
    15781 atctgtcgct ttaaaaatta ttattgaccg tgaaaaagag atccgagaat ttgttccaga
    15841 agaatattgg agcatcgacg gtaattttaa aaaagctcgc aagaaattca aagcaaattt
    15901 ctggggaatc gacggtaaga aaaagaaatt accagatgca caaagtgtaa aagaagtcac
    15961 tgctagaatt gatggaacag acttcgaagt taaaaaagta gaaaagaaag agcgcaaaag
    16021 aaaccctgcg aatcctttca ctacaagtag cctgcaacaa gaagctgccc gtaagttaaa
    16081 ctttcgaaca agaaaaacca tgatggtagc acagcaatta tatgaaggaa tcactttggg
    16141 taaacaagga acagtcggct tgattacata catgcgtact gattctaccc ggattgccga
    16201 tacagcaaaa gcagaggccg cagcatttat tgaagatacg tatggaaaag aattttctag
    16261 acatgatcac cgaaagatca aaaatgctca aggcgcacaa gatgcccatg aagctgtgcg
    16321 tccaacgagt gtgcttcgta caccagatga actcaaacaa tatttagata aagatcaatt
    16381 gaaactttat acattgatct ggtcacgttt tgtggctagt cagatgacac cagctatttt
    16441 agatacaatg aaagtgacat tagaacaaaa tggtgtcatc ttcattgcga acggttcaaa
    16501 aatcaaattc aaaggattca tgcaagtgta tgtggaaggt agagatgacg gcaaagaaga
    16561 gaaagaaaat attcttccag aacttgaaga aggcgatgta gtccaatctg ttgatattga
    16621 accaaaacaa cattttactc aaccaccagc acgttttagt gaggctacgt tgattcgtgc
    16681 actagaagaa aacggggtag ggcgaccttc cacttatgca ccaacacttg atacgatcca
    16741 aagacgctac tatgtaaaat tgacacaaaa aagatttgag ccgactgaac taggagaaat
    16801 tgtcaattca ttaatctgtg aattcttccc tcaaatcgtc gatatccatt tcacagcaga
    16861 aatggaagga gatttggata aaattgaaga aggcacggaa gcgtgggtca aagtggttga
    16921 tcgtttctat aaaccgtttg aaaaagaact gacgaatgcg gaagaaaaaa tcgaaaaaat
    16981 tcaaatcaaa gatgaaccag caggattcga ttgtgatgtc tgtggacatc cgatggtgat
    17041 aaaattaggg aaatatggga agttttatgc ttgcagtaat tttcctgatt gtcggaatac
    17101 gaaaccaatc gtaaaagaaa tcggtgtgac ttgcccagta tgtcatgaag gacaagtcat
    17161 tgaacgaaaa tctaagaaaa acagactatt ctatggttgt tcccgtttcc cagaatgtga
    17221 attcacttct tgggataaac cagtgggcag aaattgtcca aaatgtgacg gatatctggt
    17281 cgagaagaaa gtcaaaggcg gtaaacaagt agtctgtgta aatggagatt acgaagaaga
    17341 catccaaaaa taatagaaaa gaggttcttt cattgaaaat ggtaaatgtt gttggagcag
    17401 ggcttgccgg aagtgaagct gcttggcaga tcgcacaagc aggagtccct gtccgtttat
    17461 atgaaatgcg tccagtcaaa tcgactgagg cgcatcacac aagtaatttt gctgaactag
    17521 tatgttccaa ttcattaaga ggaaacagtt tggctaatgc agtagggtta ttgaaagaag
    17581 aaatgcgccg attgaattcg gtagtaatca cttcggcaga tgaaacagca gtgccagctg
    17641 gtggtgcctt agctgttgac cgcgattctt tttctgaaac aattacccga aaagtcaaag
    17701 agcatccatt agtaacagtg ataaacgaag aaattactga aataccggaa ggaatcaccg
    17761 tagtagcaac aggacctttg acttctcatc cattagcaga aagcatcaaa gcattcaatg
    17821 gttcagatgg tttttatttc tatgatgcag ctgcaccaat cgtagacaaa gctacgatcg
    17881 atatggataa agtatatttg aagtcccgct atgacaaagg agaagcagct tacttgaact
    17941 gtccgatgac agaagaggaa ttcaaagctt tccatgaggc actaatcaat gccgaagtgg
    18001 cagaacttaa atcttttgag aaagaaaaat attttgaagg atgtatgcca attgaagtaa
    18061 tggcacagcg aggattcaaa acgatgcttt tcggacctat gaaaccagtt ggattggaag
    18121 atcctaaaac tggcaaacgt ccttatgctg ttatccagct gcgtcaagac aatgctgcag
    18181 cttctttata caacatcgtg ggctttcaga ctcacttgaa atggggcgag caaaaacgtg
    18241 ttttccggat gattcctggg ttagaaaatg ctgaattcgt tcgctatggt gtgatgcacc
    18301 gaaacagctt tatgaattcg ccagaattgc tgaaaccgac ttaccaatca acgaaacgcg
    18361 aagatctgtt ctttgcaggt cagatgactg gtgtagaagg ctatgttgaa agtgcagcga
    18421 gcggactact tgctggaatc aacgccgcac gcttagcaaa agaaatggaa ccagtcgagt
    18481 ttccacaaga aacagctatt ggaagtatgg cttactacat tactcatgcg gaaggaaaac
    18541 atttccaacc gatgaatgcc aactatggtc tgtttcctga actgccagag cgcattcgag
    18601 acaaaaaagc aagatatgaa gcaattgcac aacgagcttt aaaagctaat gaggcaataa
    18661 aaacagaatt agaaaaaact gcaatcccaa atgagtaagg aaactccatg aagagggtga
    18721 gacggatagt ctcgctcttt ttcttgcgtt tttcttaaga atttctcaaa caaaatgtga
    18781 ggaattgcgc caattgtgaa caaacttaga aatattggtt aaattctgac aatttatttg
    18841 tctttttata aacgctgtgc taaattattc tctgggacaa aaggaaggtg agaaaatgac
    18901 agaagccacg tgggaacaaa aatttttgcg ttatttgatt gtcgaaagag gatattcaga
    18961 aaaaacgaaa gaagcatatg aagaagacat tctccatttc aaacattttt tggaggaatc
    19021 tggagacgct gatctgctga aggcggaata ttttacggtt cgtgtctatt tgagtgcatt
    19081 agctgatcat cgtcctagct atagcagaaa ttctatcagt cgcaagatag ccagcctacg
    19141 ttcgttttat cagtatcttc tcaaagaaga agtgataaaa gaaaatccat tttcttacgt
    19201 tcatctcaaa aagaaaaatt tgagactgcc acgttttttt tatgaaaatg aaatgcaagc
    19261 actttttgac agcgtagcag gagatagtcc tttggatttg aggaaccgcg ctttactgga
    19321 agtcctttac gggtcaggca tccgtttaag tgagtgtagc agtttgtcgg tatcagacat
    19381 tgattttgat tcagaggtca tgttgatcca tggtaaaggg aacaaagaga gatatgctcc
    19441 tctaggttct tttgctcaag atgctctgca agaatatttc agggaaggcc gccaggtatt
    19501 gatgacaaaa tatcacgaag agcatgatat cgtttttgtg aatcatcatg gaaaaccaat
    19561 cacgcctact ggaattgaat atgtcttgaa tcaaatgatc aaaaaaagca gtttggatag
    19621 tgatatccat cctcacatgc tgcgccatac atttgctact catttattaa ataatggggc
    19681 agatttgcgg acagtccaag aattgctagg acatgccaat ttatcgacta cccagatcta
    19741 tgcccatgta acaaaagaaa gtttacaaaa aaactatcgt tctttccatc caagagcgta
    19801 aaaataagga aaagaacaaa tttaggagga aacaaacatg gtcgaatcac catttcattc
    19861 aaccactatc tgtgccgtcg aaaaagacgg gaaatttgct atggctggag atggccaagt
    19921 aacaatgggt gaatctgttg tcatgaaagg cactgcaaag aaagtacgac gtatctacaa
    19981 tggagaagtt gttgtaggtt tcgccggaag tgttgcagat gcgtttactt tagaagaaaa
    20041 atttgaaggc aaactaaacg aatataacgg gaatctacaa cgcgcagctg tcgaattagc
    20101 tcaagaatgg cggacacagc aatctatgca aaaacttgaa gcgatgctga tcgtaatgaa
    20161 tgataaagaa atgcttcttg tttcaggtac aggagaagtt atcacaccag atgatggtat
    20221 cttagctatc ggatcaggag ggaattttgc gttggctgca gcccgagcaa tgaaaaacta
    20281 tgctcaaaac gacatgagtg cgaaagaaat tgcagaaaat gcgttaaata ttgccgcaga
    20341 tatctgtatc tttactaatc ataatattat cgtagaagaa ctgtaagagg tgagtagata
    20401 tgtatgaatt aaaacaaaca ccaagacaaa tcgtaagtga attagatgaa tatatcgtag
    20461 gacagcacgc agcaaaaaaa tctgttgccg ttgctttgcg taaccgctat cgccgtttgc
    20521 agttagatga aaaaatgcaa caagatatta caccaaaaaa tatgctgatg atcggaccta
    20581 caggggtcgg taaaacagaa atcgcacgac gtttggcaaa aatcgtcaat gcaccatttg
    20641 tcaaagtcga agcaacaaaa tttactgaag tcggttatgt cggaagagat gtagaatcaa
    20701 tggttcggga tttagtagaa aatgcgatcc aaatcgtaga aaaagagcag tacagccgtg
    20761 tatacacaag tgctaaaaaa aatgcagaaa aacgtttggt aaaaatcttg gtacctggta
    20821 tcaaaaaaga acagaaacaa tccactaatc aatttgaaca aatgatgaat ctttttaatg
    20881 ctagtcagca aaataccgct gaaccgcaag aagaagtgac agaagatatt cgaatcaatc
    20941 gccgtacagt cttggaacaa ctacaaaagg gtttactgga tagtcgtgaa gtaaccattg
    21001 aagttgaaga ccaaaagaaa cctgcaccaa tgatgaacaa tggattggag caaatgggga
    21061 tcgacctaaa tgaaactcta ggtgctttga caccgaagaa aaaagtggaa cggacggtaa
    21121 cagtcaaaga agctttagag ttattgatca aggaagaatc agcaaaatta gtcaaggatg
    21181 ctgatatcca tagtgaggca atcaaattag cggaagaaag tggaattatt tttatcgatg
    21241 aaatcgataa gatcacttct aaatcacagc aatctggaga agtatccaga gaaggcgtcc
    21301 aaagagacat attgccaatt gtcgaaggtt cacaagtaaa cacaaaatac gggatcatcc
    21361 aaacagatca cgtcctattc attgcttcag gagctttcca tttgtctaaa ccaagtgatc
    21421 taattccaga acttcaagga cgcttcccga tacgggtaga attagatgat ttgacagctg
    21481 aagattttgt gaagattctg accgaaccaa ataatgcttt agtaaaacaa tacgtcgcgt
    21541 tgatcggtac agaaaatgtg tcagttacgt ttacaaaaga agcaattgaa cggatcgctc
    21601 aaatcgcttt cgatgtcaac cgtgacacag ataatatcgg cgcacgcaga ctgcatacca
    21661 ttttggaaaa actactagaa gatctattat ttgaagcacc agatatgcaa atgggagaaa
    21721 taacgatcac agaagcatat gtggatgaca aacttggctc aattgcggca aatgaagatt
    21781 taagtcgcta tattttataa aaaatcggag gacgacaatg acagacttgc taacgaaaac
    21841 ccgcaagata aacacacttt tgcagcaaaa gaatactttt gacatgcaag cagatttgcc
    21901 ttacgataaa atggctgtca cactaggtgg tattcttgac agcaacacct atatcatagg
    21961 aagtgacgga gtattactag gttataatga acgtcacgat gtgaacaatg aacgaatcaa
    22021 aacaatgttt gttgagaaac aatttcctga atcttatact gatacagtgg cacagttgat
    22081 gaaaactgaa gctaatatcc caattactag tgatattact gcatttccgg tagagtcaag
    22141 agagaaatat ccatttggtc tgacgactgt cgttccgatc tttggagctg gcgaacgttt
    22201 gggtacgatt atcttatctc gtatggaaca atcgtttgca gatgacgatc tagtcttagc
    22261 agaatatgga gctacggttg tcggtatgca gatcctttat cagaagtctc gaagcatcga
    22321 agcagatgta cggagtgcaa cggctgtcca aatggcaatc aacacattat cttatagcga
    22381 attgaaggct gtacaagcta tctttgaagc attggatgga gatgaaggtc gtctgactgc
    22441 ttctaatatt gccgatagta tcggcatcac tcgttctgtg atcgtcaatg ctttacgaaa
    22501 attggaatca gctggaatca ttgaatctcg ttcgttaggc atgaagggaa cttatttaaa
    22561 agtattgaat tcccgtttta aagacgagtt agcaaaacac agttattaga agtaagggga
    22621 ctggggcaga tagaaatgtt tgttccagcc ttcccatttt aaaaagtaag tcgaagttgg
    22681 aggaatggca atgactgtta ttattcaaaa tgaccagctg atcgccgaaa tcgctgaaca
    22741 cggtgcggaa ttgatcagtc tgaaatcaaa agaacatggt ttagagtata tctggcaagg
    22801 tgatccagcg tattggggca gacatgcacc ggttttattc ccttttgttg gacgtttaaa
    22861 agatgatcag tatgtttatc aagggaaaac atatcaaatg gggcaacacg gatttgctcg
    22921 ggatatggat tttgaagtca ttgaacatgg acaagaaatg gctagtttca tcttaaggag
    22981 tacgccggag acattagaaa agtatccatt tgattttgaa ttagtgatca gctatgaatt
    23041 aggaggagac ggcatcacgg ttcattatca agtggcaaat accggtgaaa aagaaatgtt
    23101 cttttctatc ggtggacatc ctgctttcaa tgtacctctt gaagaaggat tgtcatttga
    23161 agatttctat ctggcatttt ctccattgaa atcaagatta cgcctaccat tgaaaggccc
    23221 attcatcgat atggatcaaa agacgttagg tcagacaaat acgaatctgg ctttgaatcg
    23281 tgaacttttt gagcaagatg ccttggtttt tgaaacaagg ggtctaaatg cgtacgccat
    23341 tcgaagtgaa aaatcttccc atagcgtaac tttaagctat aaagatatgc cttatgtagg
    23401 gatctggtct ccttatccaa aagaagcgcc tttcgtttgt atcgaaccgt ggtgcggaat
    23461 cgctgacacg gtgaacagta gtggaaactt gatggaaaaa gtagggatca atcaactagg
    23521 tgcccatgaa atcttcaaga caaaatattc catcacagtg aagtaagagg ttgtgaccga
    23581 gaagttagca gggaacaccg tttaccaagg tgtgtaaaaa agctatcctg taagtaataa
    23641 gggagtgtga catcgattt
//
LOCUS       DASHRQ010000037        23589 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.037, whole genome shotgun sequence.
ACCESSION   DASHRQ010000037 DASHRQ010000000
VERSION     DASHRQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.037"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            103..273
                     /locus_tag="U2M88_001657"
     CDS             103..273
                     /locus_tag="U2M88_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374044.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            493..1200
                     /locus_tag="U2M88_001658"
     CDS             493..1200
                     /locus_tag="U2M88_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM8374045.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1259..1903)
                     /locus_tag="U2M88_001659"
     CDS             complement(1259..1903)
                     /locus_tag="U2M88_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEM8374046.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            2030..2317
                     /locus_tag="U2M88_001660"
     CDS             2030..2317
                     /locus_tag="U2M88_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374047.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2425..2745
                     /locus_tag="U2M88_001661"
     CDS             2425..2745
                     /locus_tag="U2M88_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8374048.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2826..4139
                     /locus_tag="U2M88_001662"
     CDS             2826..4139
                     /locus_tag="U2M88_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEM8374049.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4136..4855
                     /locus_tag="U2M88_001663"
     CDS             4136..4855
                     /locus_tag="U2M88_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEM8374050.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4983..5534
                     /gene="raiA"
                     /locus_tag="U2M88_001664"
     CDS             4983..5534
                     /gene="raiA"
                     /locus_tag="U2M88_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEM8374051.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5756..8290
                     /gene="secA"
                     /locus_tag="U2M88_001665"
     CDS             5756..8290
                     /gene="secA"
                     /locus_tag="U2M88_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEM8374052.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8416..9517
                     /gene="prfB"
                     /locus_tag="U2M88_001666"
     CDS             join(8416..8487,8489..9517)
                     /gene="prfB"
                     /locus_tag="U2M88_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEM8374053.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9622..10308
                     /gene="ftsE"
                     /locus_tag="U2M88_001667"
     CDS             9622..10308
                     /gene="ftsE"
                     /locus_tag="U2M88_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEM8374054.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10292..11185
                     /locus_tag="U2M88_001668"
     CDS             10292..11185
                     /locus_tag="U2M88_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8374055.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11337..12785
                     /gene="cls"
                     /locus_tag="U2M88_001669"
     CDS             11337..12785
                     /gene="cls"
                     /locus_tag="U2M88_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEM8374056.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13122..13979
                     /locus_tag="U2M88_001670"
     CDS             13122..13979
                     /locus_tag="U2M88_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEM8374057.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14094..15014
                     /gene="pstC"
                     /locus_tag="U2M88_001671"
     CDS             14094..15014
                     /gene="pstC"
                     /locus_tag="U2M88_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEM8374058.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15014..15898
                     /gene="pstA"
                     /locus_tag="U2M88_001672"
     CDS             15014..15898
                     /gene="pstA"
                     /locus_tag="U2M88_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEM8374059.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15913..16719
                     /gene="pstB"
                     /locus_tag="U2M88_001673"
     CDS             15913..16719
                     /gene="pstB"
                     /locus_tag="U2M88_001673"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEM8374060.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16735..17493
                     /locus_tag="U2M88_001674"
     CDS             16735..17493
                     /locus_tag="U2M88_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEM8374061.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17506..18183
                     /gene="phoU"
                     /locus_tag="U2M88_001675"
     CDS             17506..18183
                     /gene="phoU"
                     /locus_tag="U2M88_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEM8374062.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18406..19437
                     /locus_tag="U2M88_001676"
     CDS             18406..19437
                     /locus_tag="U2M88_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEM8374063.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19434..20201
                     /locus_tag="U2M88_001677"
     CDS             19434..20201
                     /locus_tag="U2M88_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEM8374064.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            20301..21869
                     /gene="liaX"
                     /locus_tag="U2M88_001678"
     CDS             20301..21869
                     /gene="liaX"
                     /locus_tag="U2M88_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEM8374065.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22045..22353
                     /locus_tag="U2M88_001679"
     CDS             22045..22353
                     /locus_tag="U2M88_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEM8374066.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF
                     "
     gene            22353..22703
                     /locus_tag="U2M88_001680"
     CDS             22353..22703
                     /locus_tag="U2M88_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEM8374067.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
     gene            22841..>23589
                     /locus_tag="U2M88_001681"
     CDS             22841..>23589
                     /locus_tag="U2M88_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HEM8374068.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT         7767 a         4071 c         5063 g         6688 t
ORIGIN      
        1 gttagttcga gagctctttt ttttgctttg ctaccttgtt ccttgaagat tctgctgaat
       61 taagatagag ttaagttaac tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa
      121 gtagacgaag cagctgaaaa gttatatgat ttggtcaaat cagaaaaata caatattgaa
      181 gtgaatattc cgaaaaaagg cggaaaagcg gtccaggtaa aatcaaaacg cccaacgaat
      241 catacaaaaa aatggatggc taaaaaccgc taataataac aaaagatcca taagtcggca
      301 ttttcatcga ttttagggtc ttttgttatt ttctaggttg aacatatcta aaaaattgtt
      361 tctgacaata ggagtaggga gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac
      421 ctttatcttt ttcagttgtg aatcaggtat ataccagtta taatagaaga aacgaaagga
      481 cgggtgtacg ttatgtcgaa tcaattacct gtatatatac agattcatga ccaattaaaa
      541 aatgaaatcg aacagggtat atggaaagtc ggagaccgtc ttccttcaga aagagaatta
      601 gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag caattcaaaa cttggcagat
      661 gaaggaatct tagaaagaaa gatcggttcg ggtacgtatg ttgcgcgaga aaaagtacaa
      721 gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg aatcacaaaa tcgagtaccg
      781 tctagtcgga caatctctta tttcttgact tcgccaagct caagtgaaat ggaaaaacta
      841 aacttgagta aagatgacac gatcttgcga atggaacgta ttcgtttcgc agatgatgtc
      901 cctatttgtt ttgaagttgc cagtgttcca caaaagctga tcgatggcta cagcaaagca
      961 gagattactg aatcgttgta tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca
     1021 gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa ttgctgacta tttagaggta
     1081 aaacgaggag atgcagttct tcgtttgcgc cagatttctt attttgaaga cggtacgccg
     1141 tttgagtatg tgcgtacgca atacgtagga aatcgttttg agttttattt ggaaaaataa
     1201 aaaacaggtg atccctgatt ccttattaga aataaaagag aatcagaatc acctgttttt
     1261 atatatgatc agttatcagt tgctcgtgat aagaaacaga accctcttca aattggactt
     1321 gtccgttcag cagctcaatg atggattctt taaagtggtc agcacttttt tcatctacag
     1381 cacagatgac gctaacttga tcagtatatt ggatttcttt tataaaaaaa tgttggagtt
     1441 caagataatt ctgtaatttt cctagttgcg gatacgtgat catcacagag atctcctgtt
     1501 gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg agagaccgca tgagtgtatg
     1561 ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa atatcgagtg actactgcag
     1621 ctacattgat cagttgattt tttttgagga cttccaacat cggtacgccc gccgttccac
     1681 ttggttctcc atcatcgctg cttcgctgga tttcattttt ttctccaatg atgaaggcgc
     1741 tgcagttatg gttcgctttc caatgttctt tttttatttc ttgaatgaaa ctttttgctt
     1801 ggtcctctga ttctattcgt ctaagagaac agataaagcg tgattttttg atctcaatct
     1861 cactttgtcc attttctttt attgtacgat agcgctcgat catcttgtca cctctttttt
     1921 tcattttctt tattcagtat aaataaagtt ctaggttgtt tcaacactgt aacggttgcg
     1981 caattgctgt agtatgcgat aataaagaca aaggcaggtg gatctgggga tgcaggaacg
     2041 aatgaaaaag tacgatgcaa ttacgcatta tttgaagaac aatggcggct ctcaagtaac
     2101 gttgaccttt acccaatttg acgaactgct gtttcccagt aacggacttc ccaaaacagc
     2161 aagggaaagt acagattggt gggcaaatga ttataaacac cctgaaaaag gtgcctatgg
     2221 ctggataaat gcaggctatg aagtagttgt catcaatttg gacaaagaat atgtcgtgtt
     2281 caataaatta gtgaaatcta gttggctatt tgattaaata aaccgcgttt agaaaacgtt
     2341 gacaaatttc tttcttttta tataatagag taacagacgc gaagaaaacg tttgcatagt
     2401 ttgttttaaa aaggagagaa aagtatggct aaaaaaacaa tcatgcttgt atgttcggct
     2461 ggtatgagca caagtttact agtaacaaaa atgcaaaaag ctgctgaagc aaaaggaatc
     2521 gactcggata ttttcgctgt gtccgcttct gacgcagata acaaccttgc aaataaagac
     2581 gtggatgtgt tgttattagg cccacaagtt cgctttatga aagctgattt tgagaaacgt
     2641 ctggaaccaa aaggcattcc tttagatgtc atcaatatgg ctgattatgg tatgatgaat
     2701 ggcgaaaaag tgttagatca agcaatcacg ctcataggtg aataaataga acagtataac
     2761 tatcgaacaa gcgatccaaa aaatagatcg cttgtttttt cgtatatttt tttagggcga
     2821 agaatatgaa tcatttttgg ggtagaaggc ttctgacgag agatattgaa gcaatagact
     2881 gtgaggaggt aaatttacca aattatgaga agatcgcagc agtggaacgt ttgggcaact
     2941 ggattctttg tcatcgctgt ggaatgagga cgtccgtcat tgatggacag cttgcagatt
     3001 acggttattt ttgtacacat tgtctatatt tagggcgatg cgacactcaa caagatttat
     3061 atttgtttga tcagcctgaa gcaaaacctc gagaagtcgt ctttttttgg acaggacagc
     3121 ttactgaaaa acaaacggaa atctcaaaga gaatactttg ccattcagaa aaaagacatc
     3181 atcttatttg ggctgtaaca ggtgcaggga aaacagagat gctttatcct attttagtgg
     3241 aaactttaaa agctggagga agagtagcga tttgtacacc gcgagtcgat gtttgtaatg
     3301 aattgttttt acgctatcgt tatgtttttc cagaagaaaa aatcacgttg ctccatggga
     3361 atacagcaac ggcttatacc tttacttctt ttgttatctg tacgattcat cagctatacc
     3421 gtttttatcg gtcctttgat ttaatagtga tcgatgagat cgatgcgttt ccttataacg
     3481 gggatgcagg attagctcat gctgtgcaaa cagcgattaa accagacgga cgattcatct
     3541 acttaagtgc cacacctgat aaaaagttac taaaggaaat caagcagacg tttgacatcc
     3601 acaaacttgc tttacgcttt catcggcgtt tgcttcctga acccgtcttg gttttcatga
     3661 ataattggcg acaaaaatgc aagaacaaga agaagataaa atctttggtg cggatcattc
     3721 atcaattaat taaagaaaac catatccttt tattttgtcc aagtatcacg atgatgcatc
     3781 gtttgaaagc cacacttcaa gaggctcttc cgagatatga gatcacagat gtttcttcaa
     3841 gagacaatca gcgggcggaa aaagtccaga aaatgcgaga agcaaaatat gatatactgt
     3901 taaccacgat gatccttgaa cgtggagtca catttgaaag aatatccgtt atcgttttag
     3961 gagcggatca tccggttttt actaaatctt ctttggtaca aatcgcagga agagccgatc
     4021 gtaaagggcc ttatacgaac agtcaagtat actttttcta tgaagaaaaa actgcagcta
     4081 ttcgaaaagc ttgtcaggaa atcaaagaaa tgaatgaaga agggaagaag atgctgtgag
     4141 gtgcagttgc tgcagtaaag atatcttcca aaatttaaca ttcagcgaga ttattggtgt
     4201 ttcgaaatac agattacctc agacttgttc agtttgtcgc gcacaatttc atttattgga
     4261 aacaagaggc tgttctggct gtcaaaaacc aatccatttc aatctagaga gtacgaaaaa
     4321 gaaagtaaag tctattcccg tttactgcag tgactgtcaa gaatggaaga agaaatatcc
     4381 gacctatgat tttcagcacc gtgccttttt tgtgtacgat gaaaatatga aggcctggct
     4441 caatcaatac aagtttatgg gagatatccg actagcaggc acctttgcat ataaatggac
     4501 gcagataaaa aaagaatacc cgacatacat ctattgtcca atacctttat ctgaaaaaag
     4561 gcgaacagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa gaattccttt
     4621 tcagatgcta ctcaaaagag agaagcatct gctggctcaa gcaaaaaaca cgcgaaagga
     4681 aagattggag atgcctcaac cttttgaact tgcagtcacc ttttctaaga tacaaaataa
     4741 aaaaatattg attattgatg atgtctatac aacaggacag actcttttcc acgcagcaga
     4801 ttgtttgcag gtcgcacagc cgaaaatcat ccgaaccatt tctttggcga gatgaattag
     4861 atgtggaaat ttgatagcga tttaatttgc aaataaccta gagttcgata taatagaatt
     4921 aagaagaggg aaagagtggt ccttctcctt cttgattgtg gtagacgaaa ggggtaattt
     4981 ttatgtttag atataatgta cgcggcgaaa atatcgaagt aactgaagcg attcgcgact
     5041 acgttgagaa aaaagtaggc aaactagaac gctatttcag tgacgcaccc gatgcaacgg
     5101 cgcatgtgaa cttaaaagtt tatacagaaa aaacagcaaa agtggaagta acgatcccat
     5161 taccttatct tgtattacgt gccgaagaaa cttctccaga tttatacgca agtatcgatt
     5221 tggttgttga taaattggaa cgccaaatcc gcaaattcaa aacaaaaatt aatcgtaaat
     5281 cccgtgaaac gggcatgaat actgcagatg ctgctgcttt attcaatgaa gaggacgaaa
     5341 acgaaaatga ttctgaatta gatatcgtac gtacgaaacg cctttcattg aaaccaatgg
     5401 atagtgaaga agcagtctta caaatgaaca tgctgggaca taacttcttt atttttgaag
     5461 atgcagaaac aaatggtaca agtatcgttt accgccgtaa agatggaaaa tacggactga
     5521 ttgaaacaga ctgatcagcc ttacttaaat aacaacgaaa acttgcagca gctactgaca
     5581 gctgccgcaa gtttttttga gtttcctcct cttttagatg aaatatgaat aaattatgaa
     5641 gttcttactt taatacgctt aagatatttc aattatctat aactaatgat aaaatgaaaa
     5701 gtaaagtcta catagtctgg ataatacaca gaaggaaaag gagattacac aacgcatggc
     5761 caatttgatt cgttcactta tcgaaaacga taagaaagaa ctaaaacgtt tagatagcat
     5821 tgcaaaaaaa gttgaagcat acgctgacca aatggcagca ttaactgatg aacaattaca
     5881 ggcaaaaact gctgaattca agggcagata ccaaaaagga gaaacattag atcaattact
     5941 tccggaagct tttgcagtcg tccgagaagc agcaaaacgt gtattggggt tatatccata
     6001 ccacgtacaa ttgatgggtg gaatcgtgct tcacgatgga aatatccctg aaatgcgtac
     6061 tggtgaaggt aaaaccttga ccgcaacgat gccagtatat ctaaatgctc tgactggcga
     6121 aggggtacac gtagttaccg tcaatgaata tttagcaacc cgtgactcga cggaaatggg
     6181 cgaactttat aatttcttag gattaacagt ggggttgaat atcaactcaa aatcttcgga
     6241 tgaaaaacgc gaggcttata attgtgatat cacttatagt acgaataatg aattaggatt
     6301 tgactacttg agagacaata tggtcgttta ccgccatcaa atggtacaac gtccgctgaa
     6361 ttatgccatt gtcgatgaag ttgactcaat cttgattgat gaagcgagaa cgccgctgat
     6421 catttcaggg caagcagaaa aatccacagc tttatataca agagcagaca acttcgtgaa
     6481 acgtttgaaa gaagaagaag attataaaat cgatgtacaa tcaaaaacaa tcagtttaac
     6541 cgaagcaggg atcgaaaaag cagaagaaac attcggtttg gataacttat acgacattga
     6601 aaatactgca ttgacccatc acttggatca agcattgaga gccaactata tcatgctttt
     6661 agatatcgat tatgttgtcc aagacggaaa agtactgatt gttgaccagt tcactggtcg
     6721 gatcatggat ggacgccgtt attcagatgg acttcaccaa gcaatcgaag ctaaagaagg
     6781 cgtagagatc gaagacgaaa cgaaaaccat ggcgacgatc actttccaga actatttccg
     6841 tatgtacaag aaacttgcgg gaatgactgg tacagctaaa acagaagaag aagaattcag
     6901 agaaatttat aacatccaag tataccagat tccgacaaat cgtccggtta tccgtgatga
     6961 tcgtccagat ttactttacc caacattaca aagcaagttc cacgctgtcg tgcaagatat
     7021 caaagatcgc taccgcaaag gtcaacctgt gttagtcgga actgttgcgg ttgaaacatc
     7081 tgagttatta tcagatatgc tgaatcaaga acgtattcct catgaagtgc tgaatgcgaa
     7141 aaatcacttt aaagaagcag agatcatcat gaatgctggt caaaaaggtg ctgtgacgat
     7201 tgctaccaac atggctggtc ggggaacaga tatcaagtta ggacttgggg taagagaact
     7261 tggtggtcta gcagttatcg ggacagaacg acatgaatct cgtcgtatcg ataaccagct
     7321 tcgaggacgt gcaggacgtc aaggagaccc tggtatgtca caattctact tgtcattgga
     7381 agatgatttg atgaaacgat tcggttcaga acgaatcaaa gctttcttag atcggatgaa
     7441 gatcggtgag gaagatgctg ttatccaaag caagatgctg acgaaacaag tagaatctgc
     7501 acaaaaacgt gtagaaggaa ataactatga cacacgtaaa aacgtcttac aatacgatga
     7561 tgtgatgcgt gaacaacgtg aagtaatcta taaacagcga caagaagtaa tcatggaaga
     7621 aaaaagctta tccaagccac taatgaacat ggtcaaacgg acaatcagtc gtgtagtaga
     7681 tgcgcataca caattagaga aagaaaattg gaatttggaa agtatcatcg attttgccgg
     7741 cagtactctt gtccatgaag atagtattac tcttgcagat atcgaaggaa aaacatctca
     7801 ggagatcaaa gagtatctaa acgaacgagc acaagaagta tttaatacca aagcggctca
     7861 actaaatggt ccagagcaac tgttggaatt tgaaaaagtc gtgatcttgc gtgttgtgga
     7921 ctctaaatgg acagaccata ttgatgcaat ggatcaatta cgtcagtcaa tcggcttacg
     7981 tgcttatgga cagaacaatc cattagtcga atatcaaaca gaaggctata agatgtttga
     8041 agatatggta ggcgccatcg agtatgaagt cactcgaatc tttatgaaag ctgagatccg
     8101 tcaaaacgta cagcgtgaac aggtttctca aggaacagct tctcactcag aagacggaga
     8161 tgccaaagaa aatagtaata ctagcgcgaa aaaacagcca atccatgtga acaaggtcgg
     8221 aagaaatgat ccttgtcctt gtggaagcgg gaaaaaatat aagaactgtc atggaaaaaa
     8281 tgcaaactaa tttctgacag caggattggt gagacaaatt ctttttttaa gaattcgtct
     8341 caccttcttt ctgttatttg ctgattctgt tagactagat ataatgaaaa aagtatagtg
     8401 aaagaaggaa aaaacatgga aatatccgaa attcgaaatc tcctgacaga aatgcaggaa
     8461 aaggtcacaa gtttcaggag gtctctttga cttagatcag ctggaagaag acatcgcgga
     8521 agcagaagca aaaatggctg aacctggttt ttgggatgac agcgaagccg cacaaaaagt
     8581 gatcagcgaa aataacgcta ataaagaaac ctatgaccaa ttccattctc ttgccgaaga
     8641 actagaagaa atcgaattga tggtggagat gctgcaagaa gaacctgatg cagcaatgca
     8701 agaagaagca gaaacacgta tcaatgcatt agatgaaaaa atgaagacgt atgaattatc
     8761 tatgctgtta gacggacctt atgatcgaaa caatgcgatc gtcgaattgc atccaggggc
     8821 tggcggaacg gagtcccaag actggggcag catgctttta cgtatgtata cacgttgggc
     8881 agagcagcat ggctatcaag tagaaacact agattatcaa gcaggtgatg aagcagggat
     8941 caagagtgtg accctgctga ttaaaggaca taacgctttt ggttatctaa aatcagaaaa
     9001 aggtgttcat cgccttgtac ggatttcgcc ttttgactca gcgaaacgac gtcatacttc
     9061 tttttgttcg gtagatgtga tgccagagct tgatgaaacg atcgatatag aaatcaatcc
     9121 agatgatttg aaaatcgata cgtaccgagc aagtggtgca ggtggtcagc acatcaataa
     9181 aacagaatct gctgtacgga tcactcactt gcctacagga acagtagtag ctagtcaggc
     9241 acaacgctca caattgaaaa accgtgagca agcgatgggg atgttaaaag ctaagcttta
     9301 tcaattagaa gtggaaaaga aagaacaaga agcagctgca cttcgaggag aacaaaaaga
     9361 aatcggctgg ggttcacaga tccgttctta tgttttccat ccctattcta tggtaaaaga
     9421 ccatcggaca aacgtagaga caggaaatgt ccaagcagtt atggacggtg accttgattt
     9481 atttattgac ggctatttaa aaatgcatct acaataagaa ctcattgtta aattgaaata
     9541 aaattgtaag attttgcaag catgttgaaa cattgccatg ctataatagc taaagactga
     9601 aaaagatatg gagagaacgc catgattgaa atgaaggatg taatgaagaa gtactcaaat
     9661 ggtacgaccg ctatccgcaa tctctcgatt gtcatcgaac aaggagagtt tgtctatgtg
     9721 gttggtccgt ctggagccgg taaatctaca ttcatcaaat tgatgtatcg agaagaaaaa
     9781 gcttcaaaag gaactttgaa cgtcgcaggt catgatctaa tagcaatcaa aaaccgcgaa
     9841 gttccttatc tacgcagaga aatcggcgtg gtgttccaag actataaatt attaccaaga
     9901 aaaacagtat atgaaaatgt tgcgtacgct atgcaagtta tcggcagaaa acctcgagat
     9961 atcaaaaaac gagtaatgga agtgctggac cttgttggtt tgaaacacaa agttcgagtc
    10021 tttcctagcg aattatccgg tggggaacag caacgtgtct ctattgctcg ggcaatcgtg
    10081 aatacgccta aagtacttat cgcggatgaa ccgacaggaa atctggatcc agaaaactca
    10141 tgggaaatca tgaaattatt agatcggatc aatgcccaag gaactactgt cgtgatggct
    10201 acacataaca gtacgatcgt aaatacgatt cggcaccgag taattgctat cgaaaacggc
    10261 cgcattattc gagatcaagc ggaaggggaa tatggatacg atgattagaa cctttttccg
    10321 ccatttatta gaaagtatca agagtttgaa acgtaacggc tggatgacag ttgcttcagt
    10381 cagtgcggta acgatcacgt tgactttagt cggtgtattc atggcagtta tcatgaatgc
    10441 aactaaatta gctcaggata tagaaggaaa cgttgatgta tctgtattcg tagatatcgg
    10501 tacaaaagac gaagagatga aaaagctaga aaaagaattg aatgacttag atcatgttaa
    10561 aaaagttgaa ttttcaagca aagaacagga attgaaaaaa atccaaaacg aaatgggtga
    10621 ttcctggaaa ttgtttgaag gtgacaataa cccgttatat gatgtgtatg tagtaagtgc
    10681 tactgatcct tcttatacaa aacaagtagc agaagaagca gcagatttga agaatgtttc
    10741 tcgttctgat tacggtggag cttcctcaga tcggattttc caaatcgctg gtgcagttcg
    10801 gacatgggga ttagttgcag cggcattgct gctgttcgta gcgatgtttc tcatttcgaa
    10861 cacgattcgg atcacgattt tgtctagaca gcgtgaaatt caaatcatgc gtttagtcgg
    10921 tgcaaaaaat ggatatattc gctggccgtt tttccttgaa ggtggctgga tcggtctgat
    10981 cggtgcgatt cttccgattt tgatcatcac atttggttat gcatggtttt tccaattgat
    11041 caatccatca ttattacgtt cccattattc attgattcgt ccacaaaata tcgtttggaa
    11101 aatcaatctt ttaatggtcg gcattggtgt gatcattggt tcacttggct cgatcatttc
    11161 tatgagacgt tttttgaaat tctaatacca caatataaat aaaatgagaa agttggacag
    11221 tttcgttcca gctttctttt tttattggct aggagaatag aaggaagcgg cttttttccc
    11281 tttactttaa aaaacaactt gggtattctt aagttggtag aagaagaggt gaagccgtga
    11341 aaattatcgt tgacaatttt tttacgatcc ttttgattat gaatattttg ctttccttta
    11401 tcatcgtttt tcgagaaaga aaggaaactg ctcaaacatg ggcatggctg ctcgttttga
    11461 tgttcattcc agttgtagga tttattttat atattttttt aggaagaggg atttccaaag
    11521 agaaaatctt tgatttgaag atccaaggga aaatcggtaa gaacctggaa attgaagaag
    11581 acaaacaggc attgatgcga gggctttatc ctcatccgcc tactggacaa gtagatgtga
    11641 aacaattgat ctatatgctc acggtatttg aaagtacgct ctatacgact aaaaatgaaa
    11701 tcactttatt tacagatggt cgtgaaaaat tcgatgcgct gattgaagat atcaagcagg
    11761 cagaagacca tatccacttc cagtattata tttatcgcag tgatgcatta ggtgaagaag
    11821 ttcgagatgc attgacagag gcggcaagac gaggggtaaa agtcagagtc cttcttgatg
    11881 catggggttc cacacaagtt tcttccagct tttttcagaa tttgaaaaaa gcaggcggag
    11941 aagtcgcatt tttctttcct ctatttgttc cgtatattaa tcctcggatc aattatcgca
    12001 atcatcgtaa aatcgtagtt attgatggga aaatcggata tacaggtggg ttcaatgttg
    12061 gaaatgaata tctaggattg gtaaagaaat ttggttattg gcgagacaat catttgcgta
    12121 tttacggaga agcggtgtat accttgcaaa accgtttttt gatggactgg aattctcagc
    12181 atacaaaaga agtaggctat tctcccaaat ttttcccgtc cattcacact actggtgata
    12241 tagctgttca aatcgtgaca agcggaccag ataccgaaca tgaacaaatc aaaatgactt
    12301 atttgaaaat gatttcgtta gccaaacggg agatattgat ccaaacgcct tattacatac
    12361 cggatggatc gattcatgaa gcactgaagt tggctcttct ttcaggcgta aaagttcata
    12421 tccaaatacc taacaaacca gatcatcttc tcgtttactg ggcaacgtat tctttcgctg
    12481 cagagctgat cgagtatgga gcaaggatcg aaacatatga gaatggattt attcatgcaa
    12541 agacgatgat tatcgatggt ggcattgttt cagttggctc agcaaatatc gatgttcgtt
    12601 catttcgctt ggactttgag gtcaatacac tggtttatga tgaacgaatg gcttcacgtg
    12661 taagaaaagc tttttttgaa gactcgaaaa tttctacgca tctcacaaaa gaaatgtatg
    12721 aaaaccgagg aataatcatc aagatgaaag aaggattagc aagattgatt tcgccgctcc
    12781 tttaaaacat aaaaaggaca tgagaaccat caacagatgg agatctcgtg tccttttcta
    12841 acggggccat ttctgagagt acgtcagaaa tggagaaagc gtctcgcatg agcgaaatta
    12901 actttggggt atctcttgtt ttttggggaa agcaagaaat atgctaatat atgctattaa
    12961 aagagacaca tttcaaagcc agtatagcat agaaactttt aatgagatag ttttttttac
    13021 aattatgaga atttattttg ttatttaatg attttgattc gttttcatca agtaatgaaa
    13081 atgataaaat aaacatactt taggattaaa ggagatttct aatgaaaaaa cttaaattat
    13141 tcttaccaat aataggatta gccattcttt tatcaggctg ttcaaagtgg attgatcgag
    13201 gagaatccat tacggctgtt ggctcttctg ctttacagcc tttagtagaa acagttgctg
    13261 aaacttacca gacagaaaat cctggaaaat tcatcaatgt acaagggggc ggaagcggaa
    13321 ctggtttgtc tcaagtacag tcaggagcag tcgacatcgg aaactctgat ttgtttgcgg
    13381 aagaaaaatc ggggatcgat gcctcagcat tagtcgatca tcgagtagca gttgttggga
    13441 tcactcctat tgtgaacaag gatattggcg taaaagacat cagtatggaa aacctgaaaa
    13501 aactatttac aggcgagatc aaaaactgga gtgaagtcgg tggaaaagat cagccagtag
    13561 ttatattgaa ccgcgcttca ggaagcggaa cccgtagcac gtttgaaaaa tgggtattgg
    13621 acggagaaac agctgttcag gcacaagaac aagattcaag cggaatggtg cgccagatcg
    13681 ttgccgacac acccggtgca atcagctatg ttgctttttc ttatgtttcc gatgatgtag
    13741 atacgttgaa tgttaacggt gtagaaccga cagatgaaaa tgtgacaacg aacaaatgga
    13801 cgatttgggc atatgagcat atgtacacaa gagatcatcc aaaaaaactg acaaaagaat
    13861 ttttagacta tatcttatct gatgaagtac aagataacat cgttggagaa ctaggttata
    13921 tccctgtttc tcatatgaaa gtcgaacgtg attggcaagg aaatatcgtt aaagagtaat
    13981 ctttacacaa aatttacaaa cttagctaaa cttttacatg ttattaaaac caaattgatg
    14041 ttcaatttgt acactgagtg agtatgttaa acctgattaa gggggagtca tctgtggaag
    14101 atgtgaaaaa agtattatta acaaaatcaa aacgttcaaa aatggaacag cgtggaaaat
    14161 tcatcagttt tttatgtatc gcgctgatcg tgttggttgt attatctatt ttttattttg
    14221 tagctagtaa aggattggcc acctttttta ctgataagat caatgtattt gattttctgt
    14281 ttggtacaga ttggaatcca agccaaatcg gagcaaatgg gaaaccaatg gtaggggcat
    14341 tgcctatgat cacaggttcg tttattgtga cctttttatc tgctatcgtt gccacgccat
    14401 ttgccattgg tgcagcagta ttcatgacgg agatctctcc taaaaacgga acgaagatct
    14461 tacagccggt catcgagctg cttgtaggta ttccttctgt cgtttatggt tttatcggtt
    14521 tatcagttat cgtgcctttt gtcagaagta tttttggcgg gacagggttt ggtatcttag
    14581 ctggtacatt tgtgttgttc gttatgatct tgccgactgt cacaacgatg actgttgatg
    14641 cattaaaggc agtaccacgg cactatcggg aagcttcatt agcgcttggt gccacaagat
    14701 ggcaaacaat ctacaaagtc gtgctacgag cagccgtgcc cgggatttta acagcagtcg
    14761 tttttggtat ggctcgtgcg tttggtgaag ccttagctat ccaaatggtc atcggtaatg
    14821 cagcattgat gccaacagga cttactactc ctgcttctac attgacgtcc atcttaacaa
    14881 tgggaatcgg aaatacaatc atgggaacag ttgaaaataa tgtgttatgg tcattagcgc
    14941 tgatcttgct attgatgtca ttattcttta atatcgtgat ccgtatgatt gggaagaaag
    15001 gagagctgaa ataatgaacg caaaacgctc agataaaatt gctacaggtg ttttatatat
    15061 agtttcgggg atcatcgtat tgattttagc agctttacta ctttatatct tagttcgggg
    15121 aataccgcac atttcatggt ccttcttgac cgaaccttcc aaagcctacc aagaaggtgg
    15181 tggaatcggg atccagctat ttaactcgat ttatttgctt ttgatcacga tgatcatcag
    15241 ttttcctatt tctttaggtg cgggtatcta tctttcagaa tatgctggaa aaaactggct
    15301 aacagatgtg attcgcacgt cgattgaaat cttgagctcg ttgccttctg tcgttgtcgg
    15361 tttattcggt ttcttggttt tcgttattca attccagtat ggtttctcga ttatttctgg
    15421 tgcattggct ttgactgtat tcaacttgcc tcttttgact agaacagttg aggagtcttt
    15481 acaagcggtc cattacacac aacgagaagc aggattggcc ttaggtttgt cacgctggga
    15541 aacagtgatg aaagtggtgg ttccagaagc gacaccaggg attttgactg gtgtgatcct
    15601 aagttctgga agaatttttg gtgaagcagc tgcattgatc tatacggctg gacaaagtgc
    15661 acctgcgtta gattttacga actggaatcc attaagtgtt tcaagcccga tcagcatttt
    15721 ccgtcaagct gaaacattag ctgtccatat ttggaagatc aatacagaag gcacgatgcc
    15781 tgatggtgtt tctgtttcgg ctggcgcttc tgccgtgttg atcattgctg ttttactgtt
    15841 caacttcggc gctcgtgcga ttggcaaacg attataccga aaaatgactt cagcctaagg
    15901 agaagaagca aaatgaaaaa atataactgg aatgaaacaa atgtcatcac catgccaaaa
    15961 gagaaagaag tagcactata tacggatgat atacatgtct ggtatggcga caatgaagca
    16021 atcaaaggag tggatctgga atttgaaaaa aataagatca ctgcactgat tggtccttcc
    16081 ggctgcggaa aatctacgta tcttcgttcg ttgaatcgaa tgaatgatgg gatcgctaat
    16141 acgaaaatca ctgggaaaat catgtatcaa ggcgtagata ttaatgcgcc tcaagtggat
    16201 gtttatgaga tgcgcaaacg gattggcatg gtttttcaac gtcctaatcc gttcagcaag
    16261 tcgatttatg acaatattac atttgctttg aaacagcacg gcgaaaaaaa caaaaagaaa
    16321 ttagatgaaa tcgttgaaac aagcttgaaa caagctgcct tgtgggacca agtaaaagac
    16381 agcttggaca aaagtgcctt ggctttatct ggcgggcaac agcaacgttt atgcattgct
    16441 cgtgcgatcg cgatgaaacc tgatatcttg ctgctagacg agccggcaag tgctttggac
    16501 cctatttcga caggaacggt tgaagaaaca cttgtaaact tgaaagaaga ctatacgatc
    16561 atcatcgtga cgcataacat gcaacaagca gctcgtatca gcgactatac tgcctttttc
    16621 tatctaggaa aagtaatgga atatgacaaa actaacaaga tatttaccag accaaaaatt
    16681 caggcaaccg aagattatgt gtccggtcat tttggttaag acggaggcct caacatgaca
    16741 aaagaaatta tttcatctaa agatcttcat ctgtattatg gaaagaaaga agcactaaaa
    16801 gggatcgatc taagtttcaa tcaaggggaa atcactgcga tgatcggtcc ttctggttgc
    16861 ggaaaatcca cttatctgcg ttctttgaac cggatgaatg acctgattcc gaatgtaacg
    16921 atcaccggaa gtgttttata taaagacaaa gacatctatg gtccaaaaac agatgttgtt
    16981 gaactaagaa aagaaatcgg catggtattc cagcagccaa acccatttcc tttttctatc
    17041 tatgaaaatg tcatctatgg attgaaatta aaaggggaaa aagataaaaa agtccttgat
    17101 caagtagttg aagaaagcct gaaggcagcg tctgtttggg atgatgtgaa agacaaattg
    17161 cataaaagtg ctttatcttt atccggcggg cagcagcagc gtgtatgtat cgcacgtgtt
    17221 ttggcagtta atccggaaat catcttattg gatgaaccaa caagtgcgct tgatcctgtt
    17281 tctactggga aaatcgaaag tatgctgtta gaattaagag agcggtatac gatgatcatc
    17341 gtgacccata atatgtctca agcttcacgt atttcagata agacggcatt ctttttagat
    17401 ggtcacttga tcgaatacaa cgatacgaag aagatattca tgaatccaga aaaacaagag
    17461 acagaagatt atatttcagg tcgatttgga taaggaggaa acggaatgct acgtacacaa
    17521 tttgaagaag aattgctgaa tctccataat cagttctacg aaatggggat gatggtgagc
    17581 agcgccgtgc acaagtcagt tcgtgcatac atcaaacacg ataaaatctt agcccaagaa
    17641 gttatcgaaa acgatataaa tatcaataac atggaaacac gcttggagaa gaaaagtttt
    17701 gaaatgatcg cattgcaaca gccagttaca actgatttgc gtatgatcat tacggtcatg
    17761 aaagctagct cggatttaga acgtatggcc gatcatgcgg tgtctgttgc taaatccacg
    17821 attcgcgtaa aagggcagac gagaataccg gaaatcgaaa aagagatctc cgatatgtcc
    17881 gattatgtga aaaaaatggt ggacaacgtc ctagttgctt atgtcaaaac agacgaaaaa
    17941 gacgcacgta cgatcgccaa tatggatcaa agagtcaatg actatttcaa tcgaatctat
    18001 aatgcaacaa tcaaaaatat gcaggaaaat cctgagacag tcatcagtgg aacagactac
    18061 ttgaatgttg ccagttatct agaacggatc ggagactatg tgacaaacat ctgtgaatgg
    18121 atcgtctact tggctacagg gaaaatcact gagttaaata cgaaccacaa tgagattttc
    18181 taaactgaag ctgtgattat gccgtttgtt tatgaaaaga ggttgtgaaa aagctgttct
    18241 acatcaagta ataagaacag tcaaacaaaa aatagctcct catatttttc gtttggctgg
    18301 acttattacc gcagatgcaa gcttttgaac accgtttatt cgggaaaggg tttgtgacaa
    18361 aacatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca aaaaaaaatt
    18421 agcatcatca ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagcc
    18481 gctttatatg aagtacctgt catcgagata gaaaaagtac gaataaaaga ggatcatcaa
    18541 ttgattacag gaaaactgat gaactcggat caaaaaggag aatcgattta tttatcggtt
    18601 ccttatcaaa aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc
    18661 caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa atgatggctg gcttttcttc
    18721 ttcacaagta tcttcattgg cgccatgttg cttattggcg gaaaacaggg tctatttgct
    18781 ttaggaagtc tcatcatcaa tatcgtttta cttttacttt tgttgctcat ctttactaaa
    18841 acagaaggga tttctctatt aaataaatct atcctgttcg tcgttatagg gatgattatt
    18901 agcatattgg ctttagatgg atggaataga agcagtataa tcaaaatttc agctgcagtt
    18961 acgagtgttt ttttagcatt ttttatttgt cttttgacaa tgaatcattg gaaagaccaa
    19021 ggactgcgct atgaggaact gaattatctg acacgaccgt atcgttctgt ttttctttct
    19081 agcgtactga ttggaacagc tggaggaaca ctcgataccg ttatcacagt cattgcgaca
    19141 ttagaagaat tgactaagcg acaaccagaa ataacagcga aacagttatg gctttcagga
    19201 aggcaagtgg gtcgagatgt gattggctcg atgaccaata tccttttgtt cgtctatatc
    19261 agtgggtcag ttccatcgct tcttttatat ttaggaaatc aatggtcttt caaagaaacg
    19321 atagaacagc atttatcatt ggaattttta cgcgtcattg ctggcagctt aggaatagta
    19381 ttatcgatcc ccatctcggt cctctttttt ctgactgtaa ggaggctgaa acgatgaatg
    19441 tacttattat acttgttggg attttagcaa tcagcgtgct acttattggc ggtacgcaag
    19501 gtatgtatat tcttttaggc ttattcatca atttagggat ctttttcctg ttgttattcg
    19561 gttatcatca gaagtggccg atactggcat tgtcggtcat aggattttta ttgattgctg
    19621 tggttatctt attttttatc aacggttata atctaaaaat gcgtgcagct tttgtgagta
    19681 tactgatctt tttattttgt ttcctactat tgcttcctat cactgatttt ttagcgatcc
    19741 aaggttttac atcggtagaa ctagaagaat tatctggctt agataagact ttagcgattg
    19801 attttagatt acttacgaga tcgttgcttc tgattagtct tagtggtgct gttttagatg
    19861 cgagtgtagc aatttctagt gggacattcg aagtatatca ggcaaatccg caattatctt
    19921 tttaccaatt gcgccattct agtttcgcta ttgctaaaaa aatattggcg tcaacagtaa
    19981 tgacacttct ttttgcattc atgggaagtt ctttagcgtt gatcctctgg tttattgatt
    20041 tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa tatacgatgg
    20101 ctattcttac cacattgtcc gcactaattg ttcttccttt aacctgcgtg actacagcat
    20161 atctctttac aaaaaagaaa gaacaactca agatggagta aaagttacaa cttaagtcct
    20221 atgacatttt tactgaatca gtgcataata tggatgtaag gaaaagcttg caaacaattt
    20281 tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat tttagattta gtgaaaaaag
    20341 gaatcctttc gactgaagaa ggcttagatt tattagaaag catggcaact gaaaaagacg
    20401 aaaagcaaat caaaaaagaa gcagacaagg taactgctag tcataaagaa aaagataagg
    20461 caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga aatttctgag ccagttgatc
    20521 caaaggaaag agaaagacaa gatcaagaaa acttagaaaa gatcttagat gaactagcaa
    20581 ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc gctgacaata
    20641 aggcagcacg caatgagaaa caggaagcat tgatgcagct gaatacaaaa gaagaattag
    20701 gcgagttgac agaagaagaa ttagcacagc gtcaggcgtt agaggcagaa atcaaagagt
    20761 tagaagctag tggcgataca ctgttagaag aacaattgaa attagaagca gaattaaaag
    20821 atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt tactgataag tttgaattgc
    20881 cagatgattg gaaagatcaa gcaacagata cactgaatca agtaggcgaa aaaatgtcag
    20941 aagctggatc acagttaggc aaattcttga agaaaacatt ccaaactgtt tcagaaacag
    21001 tgaatgacaa catggaatgg aaagatgtca gcttacgtgt ccctgggatt gcgacaacga
    21061 aatttgagca tgaattttac tatgaagctc ctgcagcaag tatccttgat atcaaggcag
    21121 caaacggaaa cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt gaagctaaga
    21181 tcaaacttta cggaaaaatg ggtgctgaac catttgaagc attctctgaa cggagccaga
    21241 ttgaggttaa tgaagatcat atctccttcc aaattccaaa taaacgtgtg cgtgcagacc
    21301 ttgttttcta cttacctaaa cgagtatacg accacgcagc tattaaactt ctaaatggca
    21361 acatcatgat tgaaacattg gaagctaaag atatttatac aaaatctacg aatggtaaca
    21421 tcatcgtgga ccaattaact gcaacgatgc tggaagtaga aggtgtaaac ggcaatatcg
    21481 atattcgaaa cgggaacatc ttagattcta ttatcgaaac agtaaatggt acagtgacat
    21541 ttggtgcgac agcagaaaat ctttctgtgt ctttagtaaa tggtgatgta cgtcttacga
    21601 ttaaagaaga taatttgaaa aaagtagaag caagttcagt gaacggaaat gtcaaagtcg
    21661 ctttaccaga tggcatcggt ttggaaggac atgctaaaac aagtcttgga agtatcaata
    21721 gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga acggacaaac caaatgttgc
    21781 aattccgtcg tgtttcagat gatgagatcg cgcaagtgca actttcaacg acaacaggaa
    21841 gtatctattt aaaagataca gataaataaa gaggttgtga aagagatgtt cagccttgag
    21901 caataagaag cgattttgta aaatagcttc ccctatttta caaaattgtg ccttattgcc
    21961 gataggctat ctcttgaaca ccgattaaga agaaggttgt cacaacgatt ttcttcattt
    22021 aaaatagtag aacagaggtg agacatgatg aaaaaaaagt tgacaaaatc caataaaaat
    22081 gttgttttga caggtacttt agcagggatt ggggaatata tcgggattga tcccactgtt
    22141 atccgtgtgc tttatgtatt cctaagctta gttgcttttg gaagtccaat cattttatac
    22201 attttattag ctttgattat cccatctgcg cgttctgcac gaacaaacta tggacataac
    22261 aatgcatatt atgaaaaaaa caattatcgt gaacaaacaa aaaaacgcaa agaagcggaa
    22321 aaaatcgatg atgacgaatg gagtgacttt taatgtcgta ctttcagcgt attttggtta
    22381 atacgttaac attcatatcg ttggctgttc tttttcctgc gatggtccat gtgagcagta
    22441 tttggacagc cgtccttgcc agcttcgtat tatctgtgtt gaacatgttt gtcaaaccga
    22501 ttctgacgat cctgtccctg ccctttacat tagtcacatt gggattgttt acgtttgtta
    22561 tcaatgcagc gattcttaaa atgacttcct tttttgtagg agcagaacat tttagttttt
    22621 cttcatttgg tgcagcgtta ttcgtagcta tcttgatgtc acttgtcaat gtaatcgtta
    22681 gtgaacataa cagcagaaag taaaaaaagc tgtgccgaga tctcggcgca gcttttttgt
    22741 ttatcgcgaa ttgtaaaatt aagttagaaa aataaaaagg catttatggt acactcaaat
    22801 tgtatttccg acgaaagaaa acaaaggagt gtaaccataa atgacctaca aacatcttac
    22861 catagacgaa ctgacaatga tagaatcata ttatcttcaa cataataagc cggttgaaat
    22921 cgctaaccga atgggacgtg ctatacaaac tatttataat gtagtcaata agttcaagca
    22981 aggtaagact gctcttgatt attggcacca gtataaagaa aataagaaaa aatgtgggag
    23041 aaaagtcatt caattacctg ctcatgaggt agattacatt aaagagaaag tcactcttgg
    23101 ttggacgcct gacgtcatta tcgggcgaaa agaaaggcct gtttcatgcg gtatgagaac
    23161 actttatcgt ttattttcta aaggaatatt tgatattgac acactaccga tgaaaggtaa
    23221 aagaaaaccc aatggccatc aggaaaaacg gggaaaacaa cagtatcagc gctcaatcca
    23281 tgatcgacct gataattatc ctgatttcaa ttctgagttt ggtcaccttg aaggtgatac
    23341 gatcgttggc attcatcata aaagtgccgt cattacttta gttgaaagat tatctaaagt
    23401 cattatcacg attaaaccca acggccgtaa ggcattagat attgaaactg cccttaatca
    23461 atggttttct cgcttcccta aaaacttctt taaatctatt acgtttgact gtggaaaaga
    23521 attttctaac tggaaagcca ttagtaacca acatgatatt gatatatatt ttgcggaccc
    23581 tggaacacc
//
LOCUS       DASHRQ010000038        23573 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.038, whole genome shotgun sequence.
ACCESSION   DASHRQ010000038 DASHRQ010000000
VERSION     DASHRQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.038"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="U2M88_001682"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="U2M88_001682"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEM8374069.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="U2M88_001683"
     CDS             complement(1174..1869)
                     /locus_tag="U2M88_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEM8374070.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="U2M88_001684"
     CDS             complement(1882..2598)
                     /locus_tag="U2M88_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEM8374071.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3533
                     /locus_tag="U2M88_001685"
     CDS             2928..3533
                     /locus_tag="U2M88_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEM8374072.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="U2M88_001686"
     CDS             3858..4181
                     /locus_tag="U2M88_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEM8374073.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="U2M88_001687"
     CDS             4363..5733
                     /locus_tag="U2M88_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEM8374074.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="U2M88_001688"
     CDS             complement(5863..7164)
                     /locus_tag="U2M88_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEM8374075.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7394..9352)
                     /locus_tag="U2M88_001689"
     CDS             complement(7394..9352)
                     /locus_tag="U2M88_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEM8374076.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9529..9795)
                     /locus_tag="U2M88_001690"
     CDS             complement(9529..9795)
                     /locus_tag="U2M88_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEM8374077.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="U2M88_001691"
     CDS             complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="U2M88_001691"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEM8374078.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="U2M88_001692"
     CDS             complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="U2M88_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEM8374079.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13139..13702
                     /locus_tag="U2M88_001693"
     CDS             13139..13702
                     /locus_tag="U2M88_001693"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEM8374080.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13955..14476)
                     /locus_tag="U2M88_001694"
     CDS             complement(13955..14476)
                     /locus_tag="U2M88_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEM8374081.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14479..15321)
                     /locus_tag="U2M88_001695"
     CDS             complement(14479..15321)
                     /locus_tag="U2M88_001695"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEM8374082.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15314..16039)
                     /locus_tag="U2M88_001696"
     CDS             complement(15314..16039)
                     /locus_tag="U2M88_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEM8374083.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16215..16511
                     /locus_tag="U2M88_001697"
     CDS             16215..16511
                     /locus_tag="U2M88_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374084.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17079..18671
                     /locus_tag="U2M88_001698"
     CDS             17079..18671
                     /locus_tag="U2M88_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEM8374085.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18684..19424
                     /locus_tag="U2M88_001699"
     CDS             18684..19424
                     /locus_tag="U2M88_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEM8374086.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19592..20941
                     /locus_tag="U2M88_001700"
     CDS             19592..20941
                     /locus_tag="U2M88_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8374087.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21069..21659)
                     /locus_tag="U2M88_001701"
     CDS             complement(21069..21659)
                     /locus_tag="U2M88_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEM8374088.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21806..23158
                     /gene="brnQ"
                     /locus_tag="U2M88_001702"
     CDS             21806..23158
                     /gene="brnQ"
                     /locus_tag="U2M88_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEM8374089.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6751 a         4746 c         4490 g         7586 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcgaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattcta tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 ctttcccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat gatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aacggtgttc aagtagctga cattcgacat tagttcaggt
     7381 caaagcgcta ctttcacaac cgctctttca accatgtttt ccgagtattt ccaatgataa
     7441 gcggaacagt ttcatgtttt acttcgctgt actctagccc aaaccatgtt tctggcgagg
     7501 tagcgatttt tttgattgcc agttttgcct gcatgatttg acgttgccat tttcctagag
     7561 ttgtcacatt ggaaactact tcatcaatca ggacaaattg gaaatcgcca acttctcgcc
     7621 ctggtgtcag agagtatctt tgtggctgtt ttggcaaacg gccttgtttc atcaaatcat
     7681 ggatgacttg gcgaacatat acgttgattt cctgttggac cctgaaacct aagaacagat
     7741 tcacttggac aataaaatca gtgtccatca tatctacttg atattctttc gtatatggct
     7801 catccgtcac ttccacatga acaaaccaat agactcgtgc tcttttaggc cgtttatcta
     7861 aaatcgaata gatgaactgt cggccaactt tatcttcagc catatcagga accaagaaga
     7921 ccacatttgt ctgatagatt ggtaaagaag tatcatcacg taattctcta agctgcggga
     7981 tataatctct caaagacaca tcctcggttg acgattcgcg aataatgttc ccttgttccc
     8041 aaataaacat cacgactaag atgatcaaac caatcagaac ggctacaaaa ccgcctcgga
     8101 agaatttgac aacacttgag ataaagaaaa cgacttctat cgcaccgaag aaaaaggtaa
     8161 ctaaatatgc aagccaacga gaatgcccat tttggataag ataaaagtaa agcagtgctg
     8221 ttgtcatcaa catcgtcaca gtgatcgata gaccgtaagc agcttccata tgatgagacg
     8281 ttcggaatgt gacaaccact agagaacagg cgatccataa aatcagattg actgctggga
     8341 tatacatttg cccgatactg ttcccaggat acatgatctt taaccgaggc agcagtttca
     8401 acttgattgc ttctgaaact agcgtaaacg aacctgtaat caatgcttgc gaagcaatga
     8461 ctgcagcgac tgttgcaaaa gcaacgccga tcacgatcca actttgaggc atcatttgga
     8521 agaatggatt caggttcttg atttgctgtg cttcagggtt ttgataaaca ttcaacaacc
     8581 acgcagcctg cccaaaataa ttgataacca aacaaatttt gacgtaaggc cagctcagac
     8641 gaatatttcg tttgccgaca tgtccaagat cggaataaag cgcctctgca cctgtcgtag
     8701 ccaggaaaat atttccaaga atgaacaagc ctaatttgtt atctgcacta aaaagcagct
     8761 ggatagcata ataaggattc agtgcacgga taactgtcca gtcttgactg aagttcatta
     8821 atcccattac gcctaaaaaa gtaaaccaag cgaacatgat tggtccgaat gcttttccga
     8881 caatatctgt accaaagcgt tggaccatga ataaaacaaa aataataact aaagtgataa
     8941 tgacgatcac gttctggtta ttaccgaacc ggtcaaaaaa ctgcggaatt cctcgcaagc
     9001 cttcaatcgc agtcgttacc gtcacagcag gggtcaatac cccatctgct agtaaggcag
     9061 ctcctccgat cattgcagga atgatcaaat atttcccacc ttttcggaca agtgtgtaga
     9121 gtgagaaaat tccaccttca ccatggttat ctgcattcaa tgcaatcacg acatatttga
     9181 ttgttgtcag tagagtcaat gtccaaaaaa tcaatgagac tgatccgata acaaaatcag
     9241 gattgattcc tgacaatccg ccattatctt caacaatcga tttcataacg taaagcggac
     9301 ttgtcccgat gtctccgtag acgactccca tcgctacaag caggccagcc attgatatct
     9361 ttttggtatt gtgcgttgtt gccacgtttt tccctcattt cttgttatat ggatatcctt
     9421 taggtaagct tgaatgatag atgatattct tatccatgac acccgctttc ctaaattgct
     9481 tctaattatc caagtaacac actgaagtgt caacttattt gaataaaatt attctttctt
     9541 tggacgttct ctgatacgtc ttgccacaaa tgaataaaga gcaaggcaaa aaattgcacc
     9601 caataatgct ggtatcaaat aaaaaccgcc gagttccggt cccagtggtc ctagtaaaaa
     9661 ttcgccgagc caactaccta aaaaaccgat aatgatattt ccagatacgc ctcccgggac
     9721 ttcctttcct aaaattgcgc cagctatcaa acctaatata ccgccgataa ataacgacca
     9781 taaaaaattc agcatctcct ttccctcctt tatccatatt tctcactttt attttacgct
     9841 ttattcagaa ggactccaaa aatatgcgcc aagaaactcc agaaaaaaag aagttgtaac
     9901 aaaagtgttc agcttatttc cgtagaagct gcttctggaa caccgtgaat cagaggttgt
     9961 gaaatcagcg gtttgacctg agtattagag aaacaaacgg aaaaatagtt cctcatattt
    10021 tgccgttttt tgagctaata tcgagggaca gctgattgaa catcgtttat tggagaggtt
    10081 gtaaaaaagc tgttctgcat caagtaataa gaacagccaa gtaaaaacag ctcctaatgt
    10141 ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcgaaa
    10201 aaaaaggact gtgacacggc ttttgtcaca gtcccctttt ttcgaataat tattgtcccc
    10261 ctgtcacctt cacgatcaca agtacattat tcctatgaat taaattattt ttcttcattt
    10321 ttcgttgaac catcttcgtt caacattgct tttcttgaca gatttacgcg tccttgttta
    10381 tcgatttcag ttactttcac aaggatctca tcacctagtt taacaacatc ttcgacttta
    10441 ttgacacgat cgtttgctaa ttgggaaata tggacaagtc catcttttcc tttgatcaag
    10501 ttgacgaatg caccgaattt ttcgatacgg acaactttcc ctagataaac ttcaccaact
    10561 ttgacttctt tggtcaaatc ttcgatgatc ttgatagctt ttttgatcat ttctgcatca
    10621 gctgaagcaa tacttacttt tccatcttga tcgatatcga ttttcacacc agtttcatcg
    10681 atgattccgt tgatcgtatc gccgccttta ccgataacat ctttgatttt agcaggttcg
    10741 atctggatca tttcgatttt tggtgcatat tggcttaatt cttcacgtgg agcagcaatc
    10801 gttgaagtca attcttctaa gatttccata cgtgcttgtt ttgcttgagt taatgcttca
    10861 gttaggattt gttctgtgat tccttgaatc ttgatatcca tctgtaaagc agtgatacca
    10921 tcttttgtac cggcaacttt aaagtccata tcgccaagat gatcttccag cccttggata
    10981 tcagttagga tcgtatagtt ttcaccatca gaaacaagtc ccatcgcaat accggcgact
    11041 ggcgctttga ttggtacacc tgcatccatc aatgctaatg tgccagcaca gatacttgct
    11101 tgggaagaag aaccatttga ttccaatact tctgctacta aacggatcgt ataaggaaat
    11161 tcttcttctg aagggattac ttgtgctaac gcacgttcac ctaatgcgcc atgtccgatt
    11221 tcccgacgcc ctggagagcc tgcacgtcct gtagaaccaa cagaaaattg agggaaattg
    11281 tagtggtgga tgaatcgttt gctgacttcc acccctaaac catcaatgat ttggtgctct
    11341 cctagaggtg cgagtgtaca agctgataaa gcttgtgttt gtcctcgtgt gaacaaacct
    11401 gatccatgta cacgtggcaa taagctgacc tctgatgaca atggacggat ttcatccaat
    11461 ttacgcccgt caggacggat cttatcgatg gtgatcaatt cacgtacgac atctttttca
    11521 agatcttctg tgatttgttt aacttctttc aacaagtgat cttcatcttc atgacctgca
    11581 aatttctcag cataagcttc gcggatatcg atcttcacct gctcgatatt atcttcgcga
    11641 gcaagttttt cttcagtcat tactgctgct ttcattgtag cgtaagaagc atcaaagatt
    11701 tcttttttca atacttcatc aacttgcagt aaagtcactt ccattttttc tttccctact
    11761 gcttgaacga tttcttcttg gaaagcaact aactctttga tagcgtcaaa accaaacagc
    11821 aatgcaccaa gcatgtcttc ttctgaaact tctttcgcgc cgctttcaac catattgatg
    11881 gcttgtttcg ttccggcaac agtcaattcg atatctgttt gttctgcttg ttctactgtt
    11941 ggattcaaga cgtactcgcc atttacacga ccaacttcta cgcctgcgat tgggccgtca
    12001 aatgggatat ctgagattga tagtgctaaa gaagaaccta gcattgctgc catagctggc
    12061 gagcagtctg tttctacact catcacgatg ttcgttactt ggacttcatt acggaagcct
    12121 tctgcaaaca tcgggcgaat tggacggtcg atcaaacggg cagtcaatgt tgcttccgta
    12181 cttggacggc cttctcgttt gatgaaacct ccagggattt tccctgctgc atacattttt
    12241 tcttcataat tgatcgttaa tgggaaaaag tctgtatctt ttgcttcttt agaggccaca
    12301 gctgcactta agactactgt atctccataa cggactaaaa cggctccgtt tgcttgtttt
    12361 gctagctgac cgacttcgat ttgtaaggga cggccgcccc atgtcgtttt gaatacttgt
    12421 ttttctgaca taacttctcc ttttccttct cgtgaaagca agacgctact aaacaaatga
    12481 aaactgccga aagccgtctt ttgagcagct ttcatttggt tagtaggagt ccatctagct
    12541 tgatcacgga attttactta attgatatac gagtaaaaaa agtgagattc atgtgaatcc
    12601 cactcttttt gactaaaatt aacgacgcaa gcctaaacgt ttgatcaatt cgcggtaacg
    12661 ttgaacgtca gttttacgta agtaagctaa caagttacgg cggtgaccaa cttttttcat
    12721 taggccgcgg tatgaatgat ggtctttttt gtgcacgcgt gcatgttcat tcaagtggtt
    12781 gatttcttca gttaatacag cgatttgtac ttctggagaa ccagtatccc cttcatggcg
    12841 agcgtattcg ttgatgattt cattcttgcg ttcttttgaa attgccattt ctttcacctc
    12901 tttcgattat tcccctgcga ctgagtaaaa cgttggtgat tcgcttaacc aagtggcagg
    12961 tcatgtgcct agagcacaca gctatacttt acctgaaacc gggttgaaaa tcaagtagtt
    13021 aatcattaag aaaaggtagt tttcaaacaa tcttactact acttttccat ttttgtggta
    13081 aactataatg cgaaaataga cttgaaacac agtcttttaa gaaaggggta catattccat
    13141 gattacaatg gatgatatca ttcgtgaagg aaatccgacc ttacgcgaag ttgcaaaaga
    13201 agtttctctt ccattaagcg aagaagatat ctctttagga aaagagatgc ttgagttctt
    13261 aaaaaacagc caagatccaa tcaaagctga agaactacac ttacgaggcg gtgttggctt
    13321 agctgctcct caattagata tttctaaaag aatcattgct gttcacgtac caagtcctga
    13381 cccagaggca gatggacctt ctctaagtac tgttatgtat aatcctaaaa ttttgagcca
    13441 ttctgttcaa gatgcttgtt taggggaagg tgaaggctgt ttatctgttg atcgagaagt
    13501 accaggatat gttgttcgcc atgccaaaat cactgtgtct tattatgata tgaatggtga
    13561 aaaacacaag attcgtttga agaattatga atcgatcgtc gttcaacatg aaatcgacca
    13621 tatcaacggt gtgatgttct atgaccacat caacgatcaa aatccatttg cactaaaaga
    13681 aggcgtattg gtgatcgagt agagaattgg caggttcctt cgatactatg ctacaagaaa
    13741 gctttataaa aaaggggagt gctcaaaagc tgcacttccc ttttttatgt ttctccacta
    13801 atcgaaattt agatagacat ctagaaatcg caattccaaa aaattctgtg tattggatac
    13861 gcatcttttc ttattttttc ttatttcttc catatgggtg tttatctcag agatgataga
    13921 atagtttcta ggataaaaag tagcagctac gatgttatcc ttcgtagagg attcctgctt
    13981 cagctagttt ttgtttgatg tttttcagca tatgcttttc tgggtagcgg atcgtatgca
    14041 gatggatgcc gcctgttaat tcggataaca aagtagcatt acttttttga taacgtctca
    14101 taaaggaatc gatatcttgt ggcgttttga tgtgcaaagt tcctgctaac tcaccgtaaa
    14161 ggggatgttc cacaattaca tcaatgattt caccaccatt ttcaacgatt agtctcagtt
    14221 cttcttctgt ttgatctttt ccatgctgga cagcgatttt actgatgtgc ccagtattgg
    14281 tttcttctgt ttcgaggata taacctcttg ctgttgccac gatactttta cctgcagctc
    14341 gtagtaaagc aatatcgcct acaatgatct gacggctgac tccaaaagtt ttcgcaaact
    14401 tgctagcact cactggtttt tctgtatttg ccaaggtttg catgatttgt tttcgccgtg
    14461 tctctccttc gatcacactt attcctcctt cattttgttt tggtattcgc gcgagtgttt
    14521 catcaaatca acatacgtgt ctgttattgt ttccttatat cccttatttt tcagcagact
    14581 aacaacttta tccaatactt gctgctctct ttttgcatca taaactgctt ggttttcttc
    14641 ttttttcact tgtcctattt tagttaccgt atccattctt ttttctaata aagcaaccaa
    14701 ctctcgatcg atcatatcga tcttatgacg taatgtttca agagactctt ctgttagttc
    14761 ttcctctact gtcttgatga tacgagcagg atttcctgca atcactacat tgtcgggaaa
    14821 agatttggtc acaaccgaac ctgcgccgac tactacgttg tcccctagag tgacacccgg
    14881 cgtgacgata actcctccgc ctaaccaaac attattaccg attttaatgg gttttgcgta
    14941 ctccagaccg ctgttacgtt tcactggatg tagtggatgc gtcgccgtgt aaagctggac
    15001 attcggagcg aacatgcagt tatcaccgat ttcaatcgtg ctgacatcta aaaatgtaca
    15061 attgaaattt gcataaaaat tctccccaac aaatatatta tatccgtaat caaaattgat
    15121 aactggctcc atatagattt ttttccctgt acggccaaaa gtctctttca acaattggga
    15181 tcgcagttcc gctgattctg cttggttgat gatttggctt tgacttcttg caaacatacg
    15241 gtcggcaacc agctcaggat cattggcaaa ataaagctcg cctgcaatca ttttttcttt
    15301 ttcggttttg aattcagtca taatctgctc ctaattcttg tatttttttg aaaacaaccg
    15361 ctccgtcttc ttgaataaac gtataaggct ccgctaaata aacaggcgtt tgattgatta
    15421 ctaagatttt ggctgacggc tggcgaaact gtaccagatc acaaaacgga tgaacttgaa
    15481 aactcgttcc aacaatgacg atcagctctg cttggctgac ggcataagcc gcctgctcca
    15541 tgactccatc ttgaaaccct tcaccatata acacgatgtc tgggcgtatt tgtcctccgc
    15601 atgcttgatg tttgtcgctg tgaagatatt ctttccagtc taccgtttgt tgacattttc
    15661 gacaatgaca ttgatacaga ctcccatgaa aattgacgag ttgcgggctt ttagcctttg
    15721 cgtgcaaacc gtcgatgttt tgggaaacaa cccaaacatt tttttctttc gctagatgtg
    15781 ctatttccaa atgaatgata ttgggttttg cttcaggatg ataaatcttt ttgacaaatt
    15841 gatagaattt atccggttca ttcaccattg cttgatgact cagcaagtat tccggctgct
    15901 ccatcccgtg gtagattcct gacaaagatc gatagtctgg cactccagaa ggtgtggata
    15961 cccctgcacc ggtcaaaaat gtaatttgct gataagctaa tatctctttt gctgcttcgt
    16021 ctactgttaa ttgtttcatg ttttgtcacc tcactttcca ttatagccca tactcgaaca
    16081 aatttattgt tgaaaagtca gaaaaatggg aataaaaaag tgttatgggc atttgagcaa
    16141 tttatcacaa tgttcactga caaaagaatg aaaacgtgtt acaatactgc tgtataaaag
    16201 tgaggtgaat cgatatgtta ccattcttta tttattgggg cgttattctg gcttgtatcg
    16261 cctggttagc gttaagcgtt tatttctctg ttttttatct tgtacgcaaa gaaaatggaa
    16321 atctttgggc ttttgcttta ttcaacgtga ttgccgcaat tgttttagcc attacattag
    16381 ccgtatatcg aacatggggc tggggaatca cccaatactc aagtttgatt tatctaatat
    16441 tagccattta tggcgtcgtt gttcttttgc aagcgatcct aggacgtgag ccaaagaaag
    16501 cggcagctta aatgatcgag atggaactgg acacaggaaa ttttctgttc tagttctttt
    16561 tttattgtat tacatacatt tttgtgtata attatactat cgtttgcaat ggatagatga
    16621 ttcacatttt gattgttttt tcggtaaaag taaagatttc tatatttatc agtatttttt
    16681 caaaaaccag cagttataga ttgacaaagc ctctcgagta tcgtaaactt tttttgtata
    16741 aataactgaa tacgagatgc ctcaatcaat gaggtagagg tcgcgagaat cattaaccct
    16801 gtggagtgaa gcaaacatgg agaagcagga atagggagga tcgccgaaat gtgcactttt
    16861 ttgcttggag tgtatgttgg gacgcaagtt aagagcttgc ggactgtctt agcagtgatg
    16921 ctaagttgcg ctatgttttg tgagaggttt atcaccttat ggaattttag cggccttttt
    16981 atatctataa atcggtcgct ttttattttt tcagataaag ctttaccgca tttcatctag
    17041 accgctcgtg tatcatataa tcagaaaggg agaaaaaaat gaaaaaaaat atggtttcag
    17101 ttgccgtact atttgctttt gtcttctcac tattcagtgt gggatctctg gcaaatgcag
    17161 atgaaaaaac accgagcgat gagtttcgcg taggtatgga ggctgggtat gcacccttca
    17221 actggtctca acaaacagat gagaataatg ccttgccaat tcaaggccaa aatagttacg
    17281 ccggcggtta tgatgttcaa atcgctaaaa aagtagcaga tggtttaggg aaaaaattag
    17341 tcatcgtcca aacgaaatgg gatggtctgg ctcctgccct tcaatcaggc aagatcgacg
    17401 cgatcatcgc cggaatgagc cctactgctg aacgacgaaa agaaattgat tttaccgatc
    17461 cttattacga atcacaatta gtagtagtag ttcaaaaaga tagcaaatat gcaaaagcaa
    17521 aaagtttagc agatctttct ggagcaaaga tcactgctca gttaaataca ttccactata
    17581 ctgtgatcga tcagatacca gatgtgcaga aacaacaagc aatggataat ttctcagcaa
    17641 tgagaacagc tttagcttct ggaatgatcg atggttatgt cagcgaacgt ccggaaggtg
    17701 taacggcaac tagtgtgaac aaagacttgg aaatgcttga attcagcggt gcagatggct
    17761 tccagacaaa cccggaagat gttcagattt ctgttggtat gcgtaaaggc gacccggata
    17821 tccaaaaagt aaaccagatt ctttctggca tctctaacga tgaacgaacc aaaattatgg
    17881 accaagcagt caaagatcag cctgctgcaa ctgatagtga agaaaaacct ggcatcattt
    17941 ctgatttcac tacgatttgg aaacaatacg gcaatatgtt cttacgaggt gctggactta
    18001 ccttgttcat cgcattgatc gggacagttg tcggtactac acttggatta ctgattggtg
    18061 tcttccgttc ggtacctgaa tcaggaaatg cactgactcg cttcttccaa aaaatcggaa
    18121 atgcacttct ttctatctat gtcgaagtat tccgcggaac accgatgatg gtacaagcaa
    18181 tggtcatctt ctacggtctg gcactcgctt ttggcatttc attgaaccga acgattgccg
    18241 ctttgtttat tgtctctctc aatactggag cttacatgac agagatcgtt cgtgggggga
    18301 tttttgcagt agatgaaggg cagttcgaag ctgcgcaagc cattggtatg acccatggcc
    18361 aaacgatgag aaaagttgtt attccacaag ttttacggaa tatcttgcca gcaacaggaa
    18421 atgagtttgt tatcaatatc aaagatacag cagttttaag tgtaatcggc gtagctgatt
    18481 tgttcttcca aggaaactct gcttccggtg cgaatttcca attcttccaa acatttacga
    18541 tcgtcgggat catctactta gtcatgacct ttacgatcac cagaatcctg cgggcggtag
    18601 aaaagaaaat cgatggtcct tcagcttata ccaagcttga agaaatcgac aattcaaatt
    18661 tgcaggaata aggaggaaga atcatgaatg aaaaaattat tgaaatcaaa catctaagaa
    18721 aaagcttcgg tgaaaatgaa gtattgaaag atatcagcgt aacagtcaat aaaggtgaag
    18781 ttgtgacgat catcggttct tccggttctg gtaaatctac tttcttgcgc tgtatcaatt
    18841 tactggaaaa accaacaggc ggcgagatca tttataatgg agaaaatgta ttagctccta
    18901 aatataattt acctaaatat cgaacgaatc taggtatggt tttccaatcc ttcaacctgt
    18961 tcaacaatat gaatgtcttg gaaaactgta tgtctggtca aacaactgtc ctgaaacgag
    19021 ataaagaaac tgccaaaaaa gtagcaatcg aaaatctgga aaaagttggt atggatcgtt
    19081 atatcgaagc caaaccagca caattatctg gtggacagaa acagcgtgtc gcaattgctc
    19141 gggcattatc aatgaatcca gatgtattgc tattcgacga acctacttcg gcgttagacc
    19201 ctgaaatggt tggcgaagtg cttaatacga tcaaagactt agcacatact ggcttgacaa
    19261 tgcttatcgt gacgcatgag atggaatttg caaaagatgt atctgaccgt gtcattttta
    19321 tggataaagg tgtgattgca gaagaaggta caccagaaga tatctttgtc catccgaaag
    19381 aagaacgtac aaaagaattc ctttcacgta ttttaaatgc ttaggatcaa tagctgattg
    19441 ggacaaagag ctgcggtgtt taccgcagtt cttttttatg aattactcat aaaaggtaaa
    19501 tttctgaaaa ttttggaatc ttagttgtgt ttttcagaaa actctcttaa tatggaagta
    19561 attaaacttt tttagttagg atgatttaaa aatgaatgaa gagcaagaac tgcagcgtgg
    19621 actgaaaaat agacatgtcc aactgatttc tattggcgga gcgattggaa cggggttgtt
    19681 tttaggttcc ggaaagtcta tccagctagc tggcccttcg atattgctcg cctatcttat
    19741 tactggatgc atctgttttt tcattatgcg tgcgttaggc gaacttctgt tatcaaacac
    19801 caataaccat tcttttctgg attttgttgc agaatatctt ggcaaaaaag cggctttcat
    19861 cactggctgg acgtattggt tctgctgggt atcgattgca atggccgatt taacagccat
    19921 cggaatgtat gtacgctact gggcgcctgg tgttcctcag tggcttcctg aactgattgc
    19981 attaggtctt cttctttgct tgaatatggt ggctgtctca ttgtttggtg agttagagtt
    20041 ttggtttgca ttgatcaaag ttgtagcgat cattgccttt atcctcgtag gagcttatat
    20101 gatcctcacc cactatacga cagatattgg cacagccagt atcacgaatc tatggtctca
    20161 tggcggattc tttccaactg gtgcaaaagg atttatctta gctttccaaa tggtaacctt
    20221 tgcttttgct gggatagaat tagttggatt agtcgctgga gaaacagaaa atcctgaaaa
    20281 agtgttgccg gaagctatca ataatattcc tatcaggatc atcttatttt acttaggatc
    20341 tctttttgtc attatggcaa tctatccatg gaacagcttg aatccagata acagtccatt
    20401 tgtagaagta ttttcggaaa ttgggatcac gattgctgct tccttgatca atttagtcgt
    20461 attaagtgca gcagcttctg cctgcaacag tgcaatatat agtacaggac gtatgcttcg
    20521 ttctttagcg caagaaggca gtgcccctaa aaaattcaaa aagttaacaa cacatcatgt
    20581 gccagggaat gctttgacat tttctactat cgtcattttc atttctgtta tcttgaatta
    20641 cgtgatgcct agtgaagtat tcacactagt aagcagtatt gccactactt gtttcttgtt
    20701 tatctggagt atgcttgtct acacgcacat gaaatatcgt aaaagtatcc tcggcaaaaa
    20761 agctcattca tttaaaatgc cgttgtaccc atttagtaat tatctggtat ttctttacat
    20821 ggcctttgtg tgtgtcgtct tgttcctagg aaaagatacg agaatcgctt tattactaac
    20881 acctgtgtgg ttcttgcttc tattactaat ctatcatatg aaatatgaac gtaagaagta
    20941 agaaagtagt tgtgacagaa gtggtcagct tcgagaaata agaaggaatt tacgaaaatt
    21001 gcttcttaaa tttttgtgaa atttcggctt atttcttgga gataaaagct tctgtcccag
    21061 cctcttttct atttccccgg aaacaaccgc tgataatagg catctgcttt tttataggaa
    21121 aaaatccaaa taccagatac aaaaaaacgt ccgaataacc cgatcaacgg tacaaaaaag
    21181 acaccggcaa tcaagcataa gttaatcatc atggagataa gacttcgacg gtcctttgac
    21241 aaaactctca cgacatggga atctggttta tttgctttca tgatttgcct catcaagata
    21301 ccaaaagaaa aactccacat cgtgtatact aagacataag ccatctcagg ccaaaaagct
    21361 tcagggttac gcccgaccca actagtaaca gaaggacata aagtcagcca aaagagcaaa
    21421 aagatattcg accagaccac tcggtaatta attcgttctg ccatctgaaa tatacgatga
    21481 tggttcaccc agatcaccgc aacaaatata aagctgatgg tataagctaa taatgtattt
    21541 cggatgaccc ataaagaatg aaagctagat gtattaggga tcttgatctc taatacaata
    21601 actctcaaaa caatggctac cactccatca gtaaatgctt ctaatctcga ctttgtcacg
    21661 atgattcctc ctcagatgat tcagcgcttt tattttatca cttacgttat taaataacca
    21721 tcgttttata tagggaattt tctgaaaatt gttgcatatt tttcgaaatt ttgttacttt
    21781 taaaaaaacc gtaaaggagt tatgtatgac aaaaaaatta tcatttaaag actatctttt
    21841 tataggctcc atgcttttcg gtctattttt tggtgctgga aatcttatct ttcccgttca
    21901 tttgggtcaa gaagcaggcg ctgctacctt ttgggcgaat ctaggtttct tggttaccgg
    21961 tatcggtctt cccttcttag gggtgattgc cattggtgta tcgaaaagct caggtgtata
    22021 tgaattagca aaacggataa atcaaacata tgcgatggtt tttaccatct tgctttactt
    22081 agttatcgga ccgttttttg ctttacctcg ccttgcaacc acatctttcg agattggttt
    22141 agcaccattt atcgatccta gcagacagac gctatatcta gcaattttca gtattgtatt
    22201 ttttgtgctg gcttggtggt tttcaagaaa accgacaaag atattagatt atgttggaaa
    22261 gttcctgaat cctgcatttt tggttttatt aggtgctttg cttctccttg cttttttgaa
    22321 tccactaggt tctgtaaatc aagcgcctat tcagccaaat tatcaagagc atgctttttt
    22381 cacagggttt actcaaggat ataacacatt agatgcgctt gctgctttag catttgggat
    22441 catcattgtc acaacaatcc aaaacatggg tgtaacaaaa cctgctgaaa tcgctaaaga
    22501 cacgatcaaa tctggtgcga tcagcattat attgatggga atcatttata ctttgctcgc
    22561 ttatttaggt gctatgagct taggtagctt tgctctaagt gaaaatggcg ggatcacgct
    22621 ggctcagatt gctgatcatt atttaggtac ttatggcagt atcttattag catttatcgt
    22681 tatattagct tgcttgaaga ccggtattgg tttgattaca gcttttgcag aaacattcac
    22741 agatttgttt ccgcaaagaa attacgcatt ctttgttgca cttgctagta tccttccttg
    22801 tttagcggca aacgtaggct tgacgaatat cattcaattt tcgttgcctg ttttgatgtt
    22861 catctatcca ttagcaatga cactgatttt actagtgtta gttggtcctt tgtttaagca
    22921 gcgtacggct gtttatcgaa tgactactta ttttacattg attgcttcga tttttgatgg
    22981 gttgaacgct tgcccagaaa ctatcaaaca aacacctatt gttcaaaatc ttttatatgt
    23041 tgcggaaagc tacttgccgt ttttcaaatt ggggatgggg tggatcgtac cagcagttat
    23101 cggttttgtt atcggactga tttggagttt cgctaagaaa gaagaagtag cagattgata
    23161 aaagaggttg tgaaagagac gttcagcctt gagcaataaa aagtaatttt gaaaaatggc
    23221 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctgacttttg aacaccgttt
    23281 attgagaggt cgtgaaaaag ccgcttagtt tcaagtatta agaaatattt tgtgaaaatg
    23341 gcttctcaca tttttgcata aatttgactt aataccgaga aactggcttt tgaacaccgt
    23401 ttattgagcg tctataaact gacctgagta ttagagaaac aaaacggcaa agtagctcca
    23461 tatactttgc cgttttgttt tgaactaata ccgaagagaa gctgatcaaa caacatttct
    23521 tttataatat agattttaga catatatatg atataaaaat agttttcgca aaa
//
LOCUS       DASHRQ010000039        23337 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.039, whole genome shotgun sequence.
ACCESSION   DASHRQ010000039 DASHRQ010000000
VERSION     DASHRQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.039"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1141)
                     /locus_tag="U2M88_001703"
     CDS             complement(<1..1141)
                     /locus_tag="U2M88_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEM8374090.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKK"
     gene            complement(1175..2545)
                     /gene="radA"
                     /locus_tag="U2M88_001704"
     CDS             complement(1175..2545)
                     /gene="radA"
                     /locus_tag="U2M88_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEM8374091.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(2608..3135)
                     /locus_tag="U2M88_001705"
     CDS             complement(2608..3135)
                     /locus_tag="U2M88_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEM8374092.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFFQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            3365..4171
                     /gene="proB"
                     /locus_tag="U2M88_001706"
     CDS             3365..4171
                     /gene="proB"
                     /locus_tag="U2M88_001706"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEM8374093.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            4168..5415
                     /locus_tag="U2M88_001707"
     CDS             4168..5415
                     /locus_tag="U2M88_001707"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEM8374094.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            5491..5835
                     /locus_tag="U2M88_001708"
     CDS             5491..5835
                     /locus_tag="U2M88_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374095.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(5952..6278)
                     /locus_tag="U2M88_001709"
     CDS             complement(5952..6278)
                     /locus_tag="U2M88_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374096.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(6651..7853)
                     /locus_tag="U2M88_001710"
     CDS             complement(6651..7853)
                     /locus_tag="U2M88_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM8374097.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            7970..8485
                     /locus_tag="U2M88_001711"
     CDS             7970..8485
                     /locus_tag="U2M88_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEM8374098.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(8684..9130)
                     /locus_tag="U2M88_001712"
     CDS             complement(8684..9130)
                     /locus_tag="U2M88_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEM8374099.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(9177..10655)
                     /gene="msr(C)"
                     /locus_tag="U2M88_001713"
     CDS             complement(9177..10655)
                     /gene="msr(C)"
                     /locus_tag="U2M88_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEM8374100.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(11009..11638)
                     /locus_tag="U2M88_001714"
     CDS             complement(11009..11638)
                     /locus_tag="U2M88_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEM8374101.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(11622..12086)
                     /locus_tag="U2M88_001715"
     CDS             complement(11622..12086)
                     /locus_tag="U2M88_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM8374102.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(12573..13841)
                     /locus_tag="U2M88_001716"
     CDS             complement(12573..13841)
                     /locus_tag="U2M88_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEM8374103.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(14072..14809)
                     /locus_tag="U2M88_001717"
     CDS             complement(14072..14809)
                     /locus_tag="U2M88_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM8374104.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(14876..15445)
                     /locus_tag="U2M88_001718"
     CDS             complement(14876..15445)
                     /locus_tag="U2M88_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEM8374105.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            15573..16445
                     /locus_tag="U2M88_001719"
     CDS             15573..16445
                     /locus_tag="U2M88_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEM8374106.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            16787..17659
                     /locus_tag="U2M88_001720"
     CDS             16787..17659
                     /locus_tag="U2M88_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEM8374107.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            17656..19613
                     /locus_tag="U2M88_001721"
                     /pseudo
     CDS             17656..19613
                     /locus_tag="U2M88_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            complement(19864..20715)
                     /locus_tag="U2M88_001722"
     CDS             complement(19864..20715)
                     /locus_tag="U2M88_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM8374108.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            20838..21305
                     /locus_tag="U2M88_001723"
     CDS             20838..21305
                     /locus_tag="U2M88_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEM8374109.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(21568..22050)
                     /locus_tag="U2M88_001724"
     CDS             complement(21568..22050)
                     /locus_tag="U2M88_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEM8374110.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(22150..23013)
                     /locus_tag="U2M88_001725"
     CDS             complement(22150..23013)
                     /locus_tag="U2M88_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEM8374111.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6870 a         4887 c         4235 g         7345 t
ORIGIN      
        1 tctttttctc tgtctctttt ttattgtcga ttcctcgtcc tgcatgtgca ggtttagcaa
       61 agatcatgcg tccagcagct gtttgaaggg cacttgttac aacgacttcg atacgttcat
      121 tcatgtaatg ctgtccatct tctacgacaa ccatcgtgcc gtcatcaagg taggctactc
      181 cttgttggcg ttctgtcccg gcttttacta ctagaacatt catcgtttca ccagggatga
      241 cgactggttt tacggcattt gccaacgcat tgatattaag aacaggtaca ttttggaatt
      301 cagagacttt attcaagtta tagtcattgg ttacaatgac accatctaaa agcttagcca
      361 atttgatcaa tttgctgtct acttcactga tatcttcaaa gtctccatcg tacatttcga
      421 cagatatacc ctcttctttt tgcaaggcat ttaggatatc taatccgcga cgaccacgca
      481 cacgtttcaa actatcccct gagtccgcga tgtattgtag ttcgtaaaga acaaaattag
      541 ggatcatcaa ggttccttct aagaatccag tctttgtaat atcataaatc cgaccgtcaa
      601 tgatcacgct tgtatccaag attttatatt tatggaaatt ttctcctgat tttcgttcta
      661 acagttgact ctcaacttga ctagctgttt cttgctcgat ttttttgctt cgtgaaccaa
      721 aaactttctt ccattcttcg attcgtgtcg ttcccattct caatcccaga tagcctaaga
      781 tgatcatcag taaaatcggc aatacactat tgacgaatgg aatttccata ttgtaaagag
      841 gggtcgagat cacaacacct aaagtcaatc cgatgatcgc accgatcgca ccgaagagaa
      901 gataagtcaa gctgacctca cttagttttt gttctacttg tttgataata gctgagatat
      961 gtttggccaa cagcaaagat aataaaaaga aaataagtgc gcctaacagg ctgttggtaa
     1021 atgtattatt cagccatccg tttgactgtt gaccgattgc ctgccaagct gagggtaaaa
     1081 atgaaatacc caagcttgcg ccgacaatga tcatcagcaa agtaatgaca cgtttttgca
     1141 tcgtttttcc tccgtttcga caatcattta ttagttagcg gaacacacgc ttcaacgttt
     1201 cagccaaagt tgctactcca actatctcga ttccttttgg cgcttcccag ttacctaagt
     1261 tgtttttagg taggtatact tttgtgaagc cgagtttttg gacttctttg actcgttgtt
     1321 caatatgact gacacgtctg atttctccag tcagtccaat ttcaccgatg aaacattccg
     1381 ttggctgtgt tcctttttct ttatagcttg aagcaatact gacagcgata gctaaatcaa
     1441 tcgctggttc atttaatttt accccacctg cagcttttaa gtaagcatct tgattctgaa
     1501 gaagcaaccc agctcttttt tccagcactg ccatgatcag agatacccga ttgaaatcaa
     1561 gacctgttgt tgttcgtttg gcatttccga acatagtagg tgtgactaat gcttggatct
     1621 ctaccaaaat tggacgtgtc ccttccattg ccacaacgat agcagagcca gttgcaccgt
     1681 ctaggcgttc ttctaagaaa atctgtgaag gattagcgac ttcttccaat ccatgctcat
     1741 gcatttcaaa aatcccaatc tcattcgttg agccaaatcg gttttttact gctcgcaaaa
     1801 tccgaaaact atgatgttgt tcaccttcaa aatataacac cgtatccacc atatgttcta
     1861 acattcttgg gccggcgatc gagccttctt tggtaacatg accgacgata aaaatggcta
     1921 tcccatttgt tttagcgatt ttgagtagtt ctgcagtggt ttctcttact tggctgacac
     1981 ttcctgccac acttgtgatg tctggctgcg tcatcgtttg gatggaatca atgataacat
     2041 aatcagggga gatatgttcg atggcacgac tgatttcgtt catatctgtt tcagcgtata
     2101 gataaaattc tgtgttgata cttccgaggc gttccgcgcg taattttatc tgctctgcgc
     2161 tttcttcgcc ggatacatat aagacttttc cgcctatggc tgctaattgc tgagaaactt
     2221 gtaacagcaa agtggatttc ccgatacctg gatcgccacc aatcaagacc atagaccctg
     2281 gcaccacacc tccgcctaac acacggttca gttcttctaa ttttgttttg atccgaggtt
     2341 cttttttagg gacgacgtct gcgatttttg ttggttttgc tttttctcct gtcaaacttg
     2401 tccgagttct gcggtccgtt gtatcttgtt cgatttcttc gaccatcgta ttccattttc
     2461 cgcaatttgg gcagcgtccc aagaacttag gcgacacata cccgcaattt tggcaaacaa
     2521 attgtacttt tgattttttt gccattgtac cgcctccttt tatcctaatc atgagacatt
     2581 aaatcaagct taagtatctt taacgtttta tttactggaa gaaccaaatc cgccttgacg
     2641 ttgagcagtc gtttgatctt gatctgcttt taaaaacggc aaaaagatcc cttggccgat
     2701 acgctcgcct tttttgatca ctacatcctt gaaaccaaag ttggtgaatt ggaacatgat
     2761 atggccttca tttgcctcat tattgtaata atcactgtca atcacaccta ctccgtttgt
     2821 caacactaaa aaccgcttca acggattgct tgaacggtta gcaagctgaa aaaattcatc
     2881 ttctcccatg taagctttga ttccagtagg cacgagggtt ggtttcaatt gtttttcatt
     2941 ttctgataca tcttttttca atccatctgc gactgtttcc aacccctttt tccagcctgc
     3001 tttccagata cttggaatca cgacctcttc tgctgcttca aaatcatagc cagcagcatg
     3061 atgtgtagcg cgctgcggta gattgatatt ttgatcttta tatttagaaa taacttcaaa
     3121 tcctcttttt ctcaccggtt ttcctcccta aataaactac tcctattcta acatatccga
     3181 ggctgtaaaa gactttcttt acaaattctt caagaaattc tgctatctta aagtaaacca
     3241 ttaattcgtt ctttttccaa acgactgcaa gttgtacaaa aaaactattt tttacatgac
     3301 tttactcgtc aaaacaagta gaatagaacg aaattaatga gtggatattt gattgaggtg
     3361 tcacatgcgt aaagaaatta aaaatgcaaa acgaattatt gtcaaagtag gaacaagtac
     3421 attgatttat cccaatggaa atatcaatct tgcggcaatc gatcagttag cttttgtcct
     3481 ttctgatctt agaaatcggg gaaaggaagt gatcttagtc tcatcaggag ctgtaggcgt
     3541 tggtatgcat aaattacatt tgtcagaacg tccgactttg attcctgaac agcaagctgc
     3601 ggcggctgtt ggtcaagccg aactgatgaa tatctataat caaagattca gtgtatataa
     3661 tcaacagacg gcacagatcc tcgtgacaag agacgtggta gattatcctg aaagtcgtaa
     3721 aaatgtgagg aatacattgg aacagctttt gattatggac acgatcccta tcgtgaacga
     3781 aaacgatact gtagcagtgg atgaattaga tcatctgaca aaatttggcg ataatgatca
     3841 gctgtcagcc atcatggcaa aacttgtcgc ggcagacctt ttgatcgtcc tttctgatat
     3901 cgacgggttt tattcagata atccgactac aaatcccgag gctcagatgt atcgctatat
     3961 cgataaaatc gatgaaaaga ttctggcaca agcaggtggt gctggtagca catttggaac
     4021 aggcggtatg cagagtaaat taaaagccgc ttctaggata tttgaagcaa acagcgcaat
     4081 gattttagca aatggaaaaa atccggcaat tatttttgat attcttgcag gaaaagaagt
     4141 aggtacttta tttaaggagg acttgaaatg actgacttga tgcaattagg acaacaggca
     4201 aaagatagcg cgcaacaact agcacttatg cctacaaacc gcaaaaatga gctacttgtt
     4261 caaatggcac aaaccatcaa aaagaatcaa caagcgatca ttgaagcaaa tcataaagac
     4321 ttagaaaaag cagtcgaaaa caatatcagt gaaacgatgc aagaccgctt gcagctgaca
     4381 cctgagcgaa tcaatgccat ggctacagag atcgaaaaaa ttgcttcgtt agacgatcct
     4441 attggaaaag tagatgagat gtggacgaac accgacggat tgagaatcgg caaaaagcgc
     4501 gtgccactag gcgtgaccgg catcatttac gaatctcgtc caaatgtcac gacagatgca
     4561 gctagtctag cattcaaatc agggaatgcc gttatccttc gcggtggcaa agaagcattc
     4621 ttctcgaatc aattattagt ccaattattg cagcaagtac tggtcagtgc tggcgaatct
     4681 ccttatgcca tccagtttgt cgatgatact tctcatgaaa ctgcaggaca gttgatgcga
     4741 ttgacagaat acttagatgt gctgattcct cgaggcggag caaaattaat ccaacgagtc
     4801 aaagaacagg caacggtacc tatcattgaa acaggtacag gaaataatca cgtatatatc
     4861 gataaagaag cacagttatc tatggcagtg aacatcatcg tcaatgcaaa agcttcccgt
     4921 ccatctgtct gtaacgcggc agaaacattg ctgatccatt ctgagatcgc accttttttc
     4981 ttgccagcta ttgaaaaaga actggtagaa cacggggtct ctttacgtgc ggatgctcgt
     5041 gcgttagaat atctagagac ggctgcttta gcagaagaca cagattggga tactgaatat
     5101 ctagattata tccttgctgt caaagtggtc gattctttgg atgaagcaat tgcacatatc
     5161 aatcggcata atactaaaca ttcagaaacg atcgtgacag atagttatgc ggctagtcaa
     5221 cgtttcttaa atgaagtgga cgcggcggct gtctatgtca atgcctccac tcgttttaca
     5281 gacggatcgg tatttggttt tggtgcagag attggtatct ctactcaaaa gttgcatgcc
     5341 cgtggtccaa tggggttaaa tgaattaact tccacaaaat atatcattta tggcgatgga
     5401 caaatccgcg aataatgttt cgtgtgaaac attattcccg ccttttgcct tttttctgta
     5461 aaggttctat ccaactagaa aggtgtcaat atgtttgaaa ttttaatgat tttttttatt
     5521 taccttattt ctctgaatat cgctgctttt ctgggagtcg gtattttatc ccttttcttt
     5581 caattcaaaa aacgaagcat agggagtcaa cgggaaaaat ggtcgcagta ttttgacaaa
     5641 atcgggcaaa aagggctggt tacacgcttg cacattagtt acatggttgc attatgctta
     5701 ttagcgacaa tcaattacta ttcttttttc gatcattcaa tcgcttatac gattacgcta
     5761 ctgattgctg ggatttttca cttatcttat aaatatcagc tgaataaaaa ccacttgaat
     5821 cggactttcc gctaaaaaat atccgtacga taagtagagt gccttgaaga tcggcaaaaa
     5881 actgccggac tttttagagg ctgtgacaaa gattttgtca cagcctcttt ttccatataa
     5941 acggcgttcg cctaccagct gcctggtaaa tctgtttcct cttcgttaag aagcgaaaat
     6001 ccgtcaaatg tttcgccatc ataataacga agcagctcat ccatggacaa gttcatcgtt
     6061 ttttcttttt cttcttccgc tgattcagcc agtagctgtt cccgatacac ctgatacata
     6121 ccagctattt tttgctgcat cgcttttcta gtttcacctt ccgcattgat tattggcaat
     6181 tctttgatat agccacgata aatcaaccga tcatctgaaa attcaatggg taattcaacg
     6241 agtgtcaacc cgacttcctg aaaatattct tccagcatct attatccccc cattcttttt
     6301 cttaaatgaa ttttagctta cctactgaaa attttccagt ttcattattt tgagaaaaaa
     6361 agggaatgtg acaaaaatcg tgtcacatcc cctttttccg aataaatggt gttcttctgc
     6421 caactcctca atcataagtc acaatattca acaaacatga gaaactgttt tttgaaattg
     6481 ttccttgtga ttcagagtta aacgacagtc tcacaacctc ttggtaaacg gcgttcaaaa
     6541 gcttgcatct gcggtaataa gcccaaccaa acgaaaaata tgagaagcta tttttgtttg
     6601 gctgttctta tgacttgatg caggacagct ttttcacaac ctctgatttc ttattcttct
     6661 tcatttgtag caaaataatt acggtgataa atgatacaac atacactgat aagaacaatg
     6721 gcacctgcaa aataaaacgc atagctttca ccaatatgtt caccaatcac acctgcaata
     6781 aatggtgcta aagctccacc gaaaaagcgg acaaaactat agctggacga agcaacgttt
     6841 cgttgcagac ctggtatttc catagcaatc gtcgtcaaca gtgtattgat aattccttgg
     6901 aaaaatcctg ctaacacaat gctgatcggt acaaggactg gcatagaagt tccagctcct
     6961 aataacagca aacaaataat gaataagaca aatgctgcta aaattgtccg ataagtcgta
     7021 aacttccgct caagagccgg tgcaacgaaa atcgaagcaa tagccagaag cacaccccag
     7081 ccaaaaaata caaaaccgac ttctaattca gaatagccaa tcaataaaaa tggcgcatac
     7141 gcaagtaaag taaagaatcc gaaattatat aacaatgcga ttagtccaac tgaacgaagt
     7201 ttatgattgc ttaacgcttt gacaccttcc caaaggccta cttttctctt tggtttctca
     7261 gtttcaggta aaagtaccgc tagggctata aaaccgcaaa acatcaaaaa tgctacccca
     7321 aaaaatggat aacgccatga accactgccc aagaccccac cgactagagg gccaacagcc
     7381 attccgcagc caagtgctgc ttcatacatc atgattgctt tttctgtgtt accaactaaa
     7441 atactgacga tcgttgctaa agcagtagag acaaataaag cattcccgac accccagccg
     7501 gcacgtaacc caatcagcat tccgatattc ccagataatc cgccaagacc agcgaataca
     7561 acgatgataa acaatcctgt catcaatgtt tttttcaaac cgatccgact agataagaaa
     7621 cctgtaaata gcatcaccac accagtgact aacatgtaac tagtaaataa cagggtcgtt
     7681 tcagctggcg tagcgtttaa ctgtgcagca atcgttttta agattgggtc tactagaccc
     7741 actcccataa aagcgatcat acaggtagct gctacaacta gcgctgaact tttccccttt
     7801 gtttcttcat ttgtttgttc ttgtatatct atttgattct ccaatatttc catccctttc
     7861 tctctattag catcatacat gatttatttt acatgtaaat atgcacatgt aaaacgtgga
     7921 aactttactg tgatataata aagaaaataa gacgagaggt aggattttta tgaatacttt
     7981 agcctatata ttattatgcg tgcttttatc caaaccacgt tctgggtatg aattgaagca
     8041 gctgatcaca attttttggg aagcccatca cagtcagatc tatacaacgc ttgccaagtt
     8101 agaccagcag ggttatgtga ccgttttgga caaagacgag cactctcaaa aaaagatcta
     8161 ccacttgact gatgaaggac gtgctcttgt agaggaatgg atcaaagagg aaactcccgc
     8221 tcctacgcaa aaagatgaat ttctagcaaa aatctatgct ttttcgacgc tcgataaaac
     8281 aacagctact ggactgctat tcactagaga acaacagctg aacaaaatca tgcaaaaaaa
     8341 tcaaaaaaaa ttgaatgacc tctcgctaga caagaaacaa gaatttggac gttatgttgt
     8401 gatccagcgc agaattatgc tttgtcagca ggaactccaa tggtgtcagc aagtaagaga
     8461 acaaatgcag cagttttttg agtaagggat aaacagaacc tttctagaaa tatctattga
     8521 agagcaaatt atcttcaata aaagagcaat acagttggaa aagaaaaagc gatccgaacg
     8581 acaggccaaa acctatcgtt cggatcacta tttttctaga actgcattca agagactatg
     8641 acaaaaagca tgccaaggcc ttttttcaaa taacagtgtt ccatcattca ttactttgaa
     8701 ttgcttttag cacacttctt gaaagcgggc caacgatgat caactgcaac ggtaaagcgg
     8761 caatcacatt cattttccaa gcattcatat aagcagagaa taattgatca ggtattcctt
     8821 gttccatgat cacaccgaat aaagacatac atgtcaccat tcctaaaacc atcaagcatg
     8881 aaatagttaa gatcatttgg accttgcttt ctggatttac tggtagttta aaggcaattt
     8941 ttttcgccag cacaccgatg atgaagacat ccaaaatgaa agccaccacg aatccaggca
     9001 ctagaccaat caacaaactt gataaatgaa gatcttgatg cagccataaa ttatacgcgc
     9061 tcatccccaa gaccatcaaa aaacacatca ttgttgtaaa aattattcct tcttttttat
     9121 ttgtaggcaa tctgtaccac tcctttaaat ttgtcacgaa aatcatcgta gcacatttaa
     9181 aaatttctcg taagtacttt ttttattttc tgccctttta tttcataaac ttcatcagcc
     9241 gtacgctcga caaagtaaga atcatgagaa gtaaacaaaa tcgttcccgg atatatttgc
     9301 attagcttct ctaaagcttc gatcgttgcc atatcaataa aattagtcgg ttcatctaac
     9361 agcaacacat tacttggctt agtaaaaagc aaagcaatcg tcagacgggt cgcttctccc
     9421 ccacttaatg tacataacgg tttcgtcaaa gtttcctgag caaaccctaa gtgattcagc
     9481 aaactacgga caagcgattc tgaaaactct gtttgtctca tgacaaaacg aatgatggat
     9541 tcttcagatg tcatttgata acccttttgc tgatatactt gaaagcttac tttcggagag
     9601 agaaggattc cttctccttg tttgcggata tgttctaata aggttgtctt acctgaacca
     9661 ttttcaccga caatcgcgat ttttttacct aaagaaaact gaaaatcacc atttaccagt
     9721 aatgttctcc cacttctttc caattggaca ttttgtccca tgatgggata gcggctgtgg
     9781 agttcaagag attttggtac tggaaaacgg atttgtttca tctgctgcgg cttggttgtt
     9841 tctcctattt tttggagtct tctctctaat gttttcgcat tcttttggat ggctttttgt
     9901 acactgtctt tttgtttaga ggaagacaac cgatccggtc tgatttgttg ctttttcttt
     9961 ttactggaca ctttttccaa cttttccgct tgttttttct tttcttggat cgatttttcc
    10021 aatcgttttt tctctttcat gatcgactct gcttctcgta gctgagtctt tttctccaat
    10081 tccttttgag tgagataggc actataattc cccgaaaact ctcggatttc tccgtctttt
    10141 acttcccaga ttttcgatgc tagctcatct agaaaatagc gatcatgact aacaaaaacc
    10201 agcgtaccat aataataacg aagctcttca atcagtcgat caatactttt tctatctaga
    10261 tgggttgttg gctcgtctag aagcatccct tgttcataag aagaaagaag acgtatcaac
    10321 tcgatctttc gttcttcccc tccgctccat ttccgctctg aagtttcttc cagtcttaac
    10381 tggaaatgac tggctaattc caaatttagg tcctttgctt cagcgagata ggttaattga
    10441 ggcaggtagt tgaaggtgat ctctgtttgg attttcccat gatcgggaaa aagttcaccg
    10501 gctattagtt tgagcaaagt cgatttaccg acaccatttt ctcctatgat ccctatacga
    10561 tcttgctgat agacacgaag agaatcaata gatagttcta gctggttgcc aaagctgact
    10621 tgtagatttg ttatatttac tgctaaattt tccatatgtt ttacactcct atttcatgta
    10681 aaacaacaat tatcgatact aaaaaagagc agaaaaatct gtttgcaaaa aaactgcatc
    10741 acatcttttc ctactctgaa cgaaataact atgactaaat caccaaatct gtcatagctt
    10801 tcacctattc tactgtcgtt cgaaaaaatg gacatactcc atccatgaaa ttgttgttta
    10861 tacatgtctt ttgaatttat ttagttgtta ttcaaaagtt cgaaacgtaa tttcatcgat
    10921 gcagtcattt ttccgacctc cttttttatt aggttaccac acacgattca aaaaaagcaa
    10981 tagctactga atagtaactg aaaacctttt attcgaacgt agtgatcttt tctggtgcta
    11041 aacgaacaga ctcgtaacct tcttctaccg cttttcctat tgataaaata ataactggaa
    11101 cataacggtc tttatccaaa ccaaatgcct ctgccaactg atctgcttcg aatccaccaa
    11161 ttggatttgt ttcatagcca tgatcacgcg caactaacat gaattgcata gccgctaagc
    11221 tagaatcgac tttgactaca tcgttcattt cttctcttga aaaactcttg taataaggaa
    11281 tgatcgcagc taattgttga tctctgactt cttttggcat cttccctgat tcgtaagctt
    11341 ggttatagat ttcttcgcca tattcgtaac attgcatatc gccaaaaatc atgaccattg
    11401 cagaagaagt atcattttgt ctcgtattga aacggatcaa aggacgcaag atttcttttg
    11461 cttcctcgct ttcagcgacc acaaagcgcc atggttgcat attgacagaa gaaggtgcag
    11521 tcgtcgcttc tttgatcatt tccagcattt cttcgtgtgg gattttgtat gtttcatcat
    11581 atacgcgaac ggatcttcta ccagtcataa ttgttgaaaa atcatttttt tgttccatta
    11641 ttattaacct ctttcgtttc tgttgttata ttgcaaagtt gggtattcaa tttatctaaa
    11701 agtgttagaa attgtttcat ttctacatcg ctgaaagccg gggatagcac ggaacctgta
    11761 tctttatggt ccatttcaca atgctcgatt ttcttctggg ctttttctgt tagcgctaca
    11821 tacacttccc ggttgttctg aggatttctc tgacgcgaaa catagccatt tgcttccaat
    11881 accttcagat gacgagtaac tgctccttga tcaatctgca acttttcttg caaactgctt
    11941 tgtagcaatg gattttcttc tttcagtctc atcatgatct cgtatctagt aagacttata
    12001 ccgatatttt cttcaaagat ctggacgatt ttttgatcgg ctaatttgat ttgatataaa
    12061 aggtgactaa tctcttctaa agacataatt tcctcctttc taataattga ctcgtcaacg
    12121 attgacttgt caataataga ataaaaaaag aaaaaagtca aagaatcagc tgtgtaataa
    12181 aaaaaacgct ctcaatagaa aagtatagag aaaagaaatt ttttgtagaa tgaacggaaa
    12241 aaatgataaa gaaagcacaa cgctaaaacc aagattattg attcttatca aactatagaa
    12301 gatagctctt ctgcagtaga aaattttaca ccgccaaaaa tttccgaaat cgccttttgc
    12361 aagaatcccg tcgaatatta gaaattggat cagtatgctt aaacagtaat aaaaaagtta
    12421 tgacaagatg ttgtcacaac cttttttgag taaacggtgt tcaaaaactt gcatctgcgg
    12481 taataagttc aaccaaacaa aaaacatgaa aatctgtttt tgtttggctg ttcttactac
    12541 ttgatgcagg gcagcttttt cactacctct ttttaataac cataaaatcc tattccttca
    12601 tatctccagc ccatcggtgt tagccaatct ctttccttag cactcaaggt atagtgatgg
    12661 gtaccagttt tttcatttgg attaaatagt cgataaattg gcttggaacc accagaataa
    12721 aaacttagac cttcataacg ccaaccgatt tttgttaaag agttgatctc agattgtgcc
    12781 attgtgtaat gatggtctcc actgtaagga ttgtataaac ggtagacagg ttgtccgctg
    12841 tttggtgctt gccaagcaat cccttcatat tcccagccta cagttggcag ataatttttt
    12901 tctccggcat tcaatgtata taaatgttct cctgtatgac ggttatacag acgatacatc
    12961 ggagaagttg aagttggtgc tgtatcatac tgtgtcagat tataggtttc gattacattg
    13021 ttcaatgtag aaccataatt aggcgcagtg gcataacgac ctgttagcca agccgtagca
    13081 tctttgtaag agttcgtatt gcttttccat gcaccggaat agtagtaaac gcctggctga
    13141 aaagaagtcg tcttcaatac cgttacgtta tcttgcaatg attctttgta cgaaggatat
    13201 tttctaaaag cggcattcac cgtaatccat tgcccattca catattccga agtaggcatc
    13261 gtaacagatt ggccattata gcttccttta atcccaaata gattgtaatt aggggcagaa
    13321 gccaaagtac tgttccccca gccactttca acgatggctt gagcaatcat gactgatgca
    13381 tataagtcat tggcacttgc caaactttgt gcagttgagg caatactatc aatgaacgct
    13441 tgttgagaat tagcagatga gcgagcataa aaagcatagc cagtatagtt ttcgaattca
    13501 gacaaatctg cttgatgggt atgtgtcaat gtttgcccat ctgtcaattg gttcgtgatt
    13561 tcttctgcat tctctggatt gttctgagct tctgctgatg tactgtcttc ggtttgtgtt
    13621 tgttctgttg tttcagttgt tggttcttcg gttgaaattg tattttctga gacagtagct
    13681 gactgcaaag tactctctgt atcgttttca attcctgttt ctgaagaact cgatgtctga
    13741 gttgcgacag tttctgtcga ttcttcagct agcactgttg tgttggcaac tgctagattt
    13801 cctaaaagaa taacgctaaa taataaattc tttgctttca tcttttttct cctttgaatt
    13861 atgaaacttt tcctaaataa cagtgtaact aaaaaagtaa aagcaagtca atgtccatat
    13921 ttaaataaca tttgtttctt tttgataaat ttgctttttc tacataaaaa gacgtaaatg
    13981 aagaatattc ttctccattc acgccttaaa ttgatggtcg tttgaataac tatttaacaa
    14041 gtaattctct ttggatatta tccgttatgg attatacttc ctgtgtcgtc ggacggataa
    14101 tcatgttact gacagacatc cggtctggcg tttctatcgc aaatgctaca gcagaagcta
    14161 tatcttcgga ggtcaatccc cattcttttt gcgcttcatg aagttcaaga gcagcctttt
    14221 tgtcgctgat ggtttgatat aattcagttt gaacagctcc tggagagatg atcgtagatt
    14281 tgatgttatt ttgccgttgc tcttgacgta atccttccat gattgcacgg acagcaaatt
    14341 ttgttccgca ataaacggcg gaatcaggat aaacaacatg tcccgccaca gaatcagttg
    14401 aaatgatatg cccagatttt tgtttttcca tgattggaag tacggcggaa attccattta
    14461 acacacccat gatgttgata tcaagcatat tttgccattc atcacggtga ccttcaacta
    14521 atggagcggt tggcatgatt cctgcattat tgtacaatac atcgattcga ccgaactttt
    14581 ccatcgctaa atcgataact gcttggactt gtgcaaaatc tcttacatct gctgtttgaa
    14641 tatatagctc agcttctggt aaagattctt ttattgcttt taggcgatct tctcggcgtg
    14701 cagctataac tagtttagcc cctttttctg ctaatagctt cgttgttgct tcaccgattc
    14761 cgcttgaagc gcccatgatg acaatcactt tttctgataa atcattcatt ctattctcct
    14821 cctgttttga aaaatctctt gcttatactc actatctctt atttcatctt actgttcaaa
    14881 taatccgttc gacaggtttg acgattttgg cagggattcc tgcaacgatg gtattgtctg
    14941 ggacatcttt cgtaacagtt gcattcgctg caacaatggc attctcgcca atcgttacgc
    15001 ctggcagaat agttgctcct gctccgatcc aagcattttt cttgatgtgg attggcttta
    15061 ctgacaagcc tcttcgttct tttggatcgg ttagatggtt caccgtgatg aggttgacac
    15121 gaggaccaat caatactgaa tcttctaaac agatgccacc taagtcaacg aacatcactt
    15181 gacgattgat aaagacattt ttccctatac taatatgttt gccgaaatct gtataaaaag
    15241 gcagcagaac agtcaccgta tcatctactt tgtcactaat gatattttct agccgtttgc
    15301 ggatatctgc tggttcatgg tacccagtat tcagcgctgc taaaggctct tcgttttcag
    15361 cttggatctg atggatcact tcaaagagcg gatccgttac tcgaatctct ttattgacga
    15421 tttgtttttc gatgaggctt gtcattttgc tccctccttt tatccttact ttataacgga
    15481 cggcttatca tgtctaatac ttataaaaca tacttaacta ttccttttaa ctatacttag
    15541 tggtatgctt ttttaaaaac aggagggaaa aaatggatat tcgtatactg acgtattttt
    15601 tagcagtagc tagagaaaaa aatatcagcc gagcagcaaa atctctgcat atcacgcaac
    15661 ctactctctc aaaacaactg aaagatttag aagaagaact tggtgtgaca cttttcactc
    15721 ggggctcccg agagatacag ttgacggatt ctggtcgata tctttattca aaaggaaaag
    15781 aaatcttgtc gttggtagaa aaaacggcga acaatttaac aaatgacgca atcatctctg
    15841 gagaaatcta cattggcgga ggcgaaacta aagcaatctc tttcatttct gatagccttc
    15901 attcgttgat caaggcgcat cctgatatcc agtttcattt atatagcggg aatgcagatg
    15961 atatcataga caaactggat agcggattac ttgattttgg actcgtaatc gaaccgacaa
    16021 acaaacaaaa ataccagtca tttcgactgc cagcccaaga tcgttgggga cttttgatac
    16081 atcgaaatca tcctttagca aagcaaaccg tcatccatcc gcaggaactg aaaactgttc
    16141 ctttgatcat ttcccggcaa acgtcagtgg acagtcaact agctgaatgg ctaggtttca
    16201 gcatagatca gttatcgatc gtcggaacat ataacttatt gtacaatgct tctttgatgg
    16261 tcgataacgg gacttttggt gcattagctc tagatgggat catcaatact tatgggacgg
    16321 aaaaaatatt catcccactc gatccgccat tacaagcttc actgaatctc atttggaaaa
    16381 aagaccagcc tctttctcct gctgctcgta aatttttaga tgtcctaacg gagaaataca
    16441 gttaagtaga gtttttttcc ttcgctttca gctttttaac aacgaataag ataagcaaaa
    16501 gatccaacga ttaaataaca taagtttctt aatttatttc ttccttttat ttctgataat
    16561 tccagtaaat tactataatt ataaaaaaag aattgacaaa acataactgt ctatataaaa
    16621 ttgattacgt taacaataat tcgtttcatg tgactagatc atactaggca tgaaaaatct
    16681 ctgaggaaca tggtttccct aggaggtttt tcgtgtctat ttttgtttgg ccgcaatcaa
    16741 ctggtctagc aaacgtgaaa accaaatctt caaaaagggt gcgcgcatga aaatcaaaaa
    16801 gatctttaat caaaatgcag tattggtaga tgacggtgga caggaaaaag tagctattgg
    16861 aaaagggatt ggatttgaca gaaaaagaaa tgatttgatt tttgatcgtg atgtcgaacg
    16921 gatatttgtc atggaaccag aaggtcaaat caagttgcaa aatttactta atcagattga
    16981 tgaacgcttt ttatttgcag cagaaacgat cattgatcat gcggaaactg ttttgatgga
    17041 aaagttgaat gaacatgtat tgatcgcttt gaccgaccac attgcttttt ctgcggagaa
    17101 cattaataac ggtatcgtca tccgcaacaa acttttaaga gaaatcgaag tgctttacag
    17161 tgatgaattc agtattgctc aatgggcagt agagtatctg acaaaagagc ttgggattcc
    17221 atatacttat gacgaagcag gatatatcgc aatccatatt cacagcggac gaagcggtcg
    17281 gaacaataac catcgcagta tccgtgaagt gacgattgta tctgacatca tccatttagt
    17341 ggaatcagaa ttggaaacag atatccattc agaaccattt tcactgaatt attcaagact
    17401 ggtgaatcat ttacgactac ttctacaacg aacccatgcg caacaatatg ctgttttaga
    17461 cacggaaatc gtagaaatgg tcaaacgaaa atacccagaa agctataaga tttctaaaaa
    17521 aatccgtgtg ttgctgacca aagaatacca gctcgcgatc acgaaagaag aactgggtta
    17581 cttggccatc catatcgagc gtttaagaag cgcaatcgta caaacaaatg ttaacaatca
    17641 tgaggaggaa aaaaattgaa agcatatcta caacgaatgg gacgttccct tcaacttcca
    17701 gttgcggtct tacctgctgc tgctttacta gtagggatcg gaaactggtg ggcatcttat
    17761 agtaacgaca ttgtcgctca ttttttacaa gctggcggta acgcagtttt aggtcaatta
    17821 cctttgctgt ttgccgttgg gttagcacta gggatgtcag tggacaaatc aggtgctgct
    17881 gccttggcag gtttggtcgc atttgaagtt ccagtaaatg ttttaaaaac tgattcagta
    17941 gctactttat tgaatatcaa accagaatca gttgatccat cttttgctca gatcagtaac
    18001 gtatttattg gtattattgc cggattgatt gcggcagctc tttataatcg tttccacgaa
    18061 gcaaaattac cgatggcttt atctttcttt agcggcaagc gtctagtgcc aatcttggct
    18121 gccttttcat gttgctgatc agtgcagtgc tactgctcgt atggcctccg gtttactctg
    18181 ctttagtttc ttttgctaaa ttcattttga gtttaggctg gggtggtgca ggtctttatg
    18241 gcttcttcaa ccgtttactg atccctacag gattgcatca agcaatgaac tccgtgttct
    18301 ggtttgacgt agcaggaatc aatgatattg gtaaattttt ggcttctgaa ggaacaaaag
    18361 gcgtaacagg aatgtatcaa gctggatttt tcccaatcat gatgttcggg cttccagcag
    18421 gagcatatgc tatttacaga aatgcacgtc cagaaaacaa agcaaaaaca gcttccttga
    18481 tgctagcagc agcttttgct tcattcttta ctggtgtgac agaaccactt gaattttcat
    18541 ttatgttcgt tgcttggcca ttatatgtct tgcatgcaat tttcactggg atttctttag
    18601 cagtgagtgc attcttccat tggacagcag gatttgcctt tagtgccgga tttgtcgatt
    18661 acttcctatc tttgaaaaat ccgattgcga atcagccatt gatgttgatc gtccaaggat
    18721 tagtaacagc agtgatttac tactttggct ttaactttgc tatcaaaaaa ttccatctga
    18781 tgactcctgg ccgcgaggaa gcagatcttt cagatgacga tactgcaacg actaattcag
    18841 acaataaata tgcggcacaa gcaagcaaaa tctacgctgc attaggcgga gccgacaatg
    18901 taacttcgat cgataactgt actacacgcc ttcgtttgca agtcaaagat acaggcctga
    18961 tcgatcaaaa taaaatcaaa gccactggcg tacctggtat gaagatcatc gacggaaaaa
    19021 atgcgcaagt catcgtaggt acagaagtac aattcgttgc agatgagatg gcaaaactac
    19081 atggtggtgc tgcagcacga cctgcacaaa caaacaccgt ggttaaaaca gaaacatttg
    19141 ccggtgaaac gatcgaacaa gatatttacg ctatcgcaaa tgggaaactg attccaatca
    19201 ctgaagtttc agatgatgta ttcgcggaaa aaatgatggg tgacggctat gctgtattac
    19261 cagaaaatgg agaaatcttc tctccaatcg ctggtacgat cacaaacatt ttcccaacga
    19321 aacatgctgt cggaatccaa acagatgctg gtatcgaagt cttgcttcat atgggcatca
    19381 acactgttga cttaaaaggc gaaccattca ctttatatgt agaagaaggt caaaaagtcg
    19441 caagaggtca attgattgct ttagttgatc ttgccgctat tcaatctgca ggcaaaaaca
    19501 cggatatggt cgttgtattc acaaatggcg acaaagtcca aagcttagaa atcgaacccg
    19561 cacgtgatgt aaaagcaaat gataaaatcg gctctgtttc caacaaagcc tgagccatcg
    19621 tcttccccag acttttttat cgataaacaa acaggagctg agacaaaaat cttgtctcag
    19681 ctcctttttc cgaataaaaa cggcgttctc ctgccaactc cgcggtaata agtccagcta
    19741 aacgaaacat atgaagagct attttttatt tgactcttct tattacttga tgcagaacag
    19801 ctttttcaca aaatttttct tcatactcaa aactaatcgg ctttttcaca acctctttat
    19861 acattattcc tgatccaata tttcttttaa tgctgcttca agagaagtta atggttttcc
    19921 caatactttt tctaaatcat cagaggaaat agctaactga tcctttttaa tatcatgttg
    19981 gatcgctaaa aacatgtcag ctgcttcttg agggaatccg gcttctttta gattttcgac
    20041 aaacgctgca tcacttgctt caataatttc taactcttta cctgtcgcac gctctaatgc
    20101 cttggctaat tcttcatagt cagttagatt gccggataat tctaagattt ctggagagtc
    20161 actttctact agtacttttg ctgcagcttc tgcatattca cgtttcaatg cccaacctgt
    20221 ttttccattt cctgcagcat aaacgaattg accagttttt aaagcctgcc caatgatagg
    20281 taattcattc tctacatacc aattattccg taaaaatgta tgggaaatac ctgaatcctt
    20341 aatcattttt tcagtgaatt ggtgatctgg cgctaataca ctgatcgagt gatcggcatc
    20401 tgcaaaactt gtataagcga tataagatac cccagctgct tgtgctgcct tcactacatt
    20461 cttatgttcg gcttgacgat ttcctggaac gccagagaca aaaagcaatc gatctattcc
    20521 tttcaacgct tctttcattg agtctaaatc atcataatca ccaatacgta tattaaatcc
    20581 agcttctttc agtgcttctc ctttttcttc actacgtact agtacataga tttcattttg
    20641 tggaaccatc ttttttaaga attccagcgc ataaccacca aaacctccag ttgctcctgt
    20701 tactaaataa tgcatatttg ccactcccta ttctttttat ttgttaagat ttacagctgt
    20761 acatttataa attacacctt tatcataaag agtgtggtaa gataagtcaa gcaaaaaaaa
    20821 tgaaaggatg acaactgatg aagtattctc tgcaatttag tgatgcaatc catgtacttg
    20881 cctatattga aatgtttcaa gatacccgat cactttctag tgagatgatt gccagtagta
    20941 tcgaaacaaa tgcagcaaat gtacgtaaaa tcatgagtca gctcaaaaaa tccggtttga
    21001 tctttacaaa aaccggaaaa ccgcagcctt cccttgccaa aagtccagat cagatttctt
    21061 tactagatgt ctatcgcagc atcgaaggta atacacagct tatccaagtc gatccaaaaa
    21121 caaatccaaa ttgcgtcgtt ggtgcgaata tccaagaagc tttagaagaa agctacgctc
    21181 ggctgcagaa aaaggcagaa gaagaaatgg ctaagatcac acttgatagt ctgatacata
    21241 aaatagccgt tttggaaaaa gaaaaaagac cagaaaacac aggcctcgta gagagatttt
    21301 tataattctc tctactgtgc ctgcattttt ctgatcggaa tcgtctacat aaattctttt
    21361 attctccggg ggacaaaagc cttgtcatgc ccctttttcc tctctaatgc tcaggtcaaa
    21421 gcgctgaagt catgacgtct tgataaacgg tgttcaagta gctgaccctc ggcattagtt
    21481 ctgaaaaatg caaaatatgg aaagctattt cccatttttc ctctctaatg ctcaggtcaa
    21541 agcgctactt tcacgacctc tatattatca tggtttattg actaccggta tccagatttc
    21601 tcccatatat ttcccgttgg taactcccat ttcgactaca gcatttggac ccccaacata
    21661 ggcgtaatct tctacttctc ttaaaacatg cccaaaggcc gtattcgtca aatcatttct
    21721 taattcttca ggcgattctg ctaaagcgct tacaacaaga tacgttcctt ttgggaatgg
    21781 gatgattcgc gtagtgtttg gaacatcctg actcgtttga acaccgacat agtacatcat
    21841 tttcccgtta tagggttcgt tgactgtaaa gaaataatga ttttcagcag cttgtttcaa
    21901 tgcatctatt gttccattac cgataacttt ttgctcaaat gcttgtttct ctgcagccaa
    21961 accgacaaaa tccgtattgc tactcttcaa ttccacacca atacccgtca ccgtaaaatc
    22021 tggtttttct tcgatcctat actttgccat ttctcccacc ctttctatta tattaagttc
    22081 atcttacata aaattaaagg gaaatgacac gattacgctc ttttttctag ggatattttt
    22141 attttttaac tatttttttg ctttcactgc aatccaaatc tcactgtaaa cattactgaa
    22201 atcctctccc cattttgtaa aagagatctc gggagcttcg actaacccat aatctgaaga
    22261 aggaagccat tctgcataga ttttccccca agtatcttgt aaagtttggg gaaatggccc
    22321 tttattagga aaaatggccc aagtgtgttc ttctatagca agcatttcta aatcagcgta
    22381 cggattttct tttgtcgtag caaagccaat catgtgcatc aaactgccct tttcttccaa
    22441 gcggtctcca tcaaatccat aagaaacgtt gattacttga tttggatata aatctcctaa
    22501 cttatgcatc tcttcccttt gagtctccgt gatcgtctgc gctaactttt gtatttcttg
    22561 attgactcct tcaaatttga caggaacttt tttcgccaca cctgccacat aaaactgttc
    22621 tttcttttcg atacgaaatt ccatcgattt tcctcctctg acatcaataa aaaatgtcaa
    22681 gcgtggaaaa gatttttgta tgccgtatct gctgatttct gaaggcagat atcctgacca
    22741 atcacgaaaa gctcttgaaa aaccttcgac tgtttcatat ccatatctaa acgccgtttc
    22801 tgtcactctt gatccatcta gaagatcgcg attagccaaa gccaatctac ggtttttgat
    22861 atagtccatt agcgacatcc ctgcaataaa cgtaaagatg cgttttaatt gatattcaga
    22921 gattcctgta ttttcggcta ttattttata ggaaagtcct tctgtcaaat gttcttcgat
    22981 gtaatcgatc aattggttca gatctttaag catatttatc ttcctccttg attgcctttt
    23041 tatcatatta tcaaagaaag tcaattactt gatgaatcag gaaccaattt agtcagatgt
    23101 agaaaatgtt gtatggatct aaagaaaatt ctcaagcagc gtttcgtaac ggataattgg
    23161 tattatctgc ctatctatgt tttaccttta gaaaaataaa ttttggggaa gacagattct
    23221 aagtaaacaa agcggtaaaa atatctttta ttcccttagt taatccgctt actacctact
    23281 gtacaaagca aattgttgtt caaactgctg atcagaacgc tgcttggatt tttcttt
//
LOCUS       DASHRQ010000040        22874 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.040, whole genome shotgun sequence.
ACCESSION   DASHRQ010000040 DASHRQ010000000
VERSION     DASHRQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.040"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            238..2469
                     /gene="pflB"
                     /locus_tag="U2M88_001726"
     CDS             238..2469
                     /gene="pflB"
                     /locus_tag="U2M88_001726"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEM8374112.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            2713..3474
                     /gene="pflA"
                     /locus_tag="U2M88_001727"
     CDS             2713..3474
                     /gene="pflA"
                     /locus_tag="U2M88_001727"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEM8374113.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            3559..4485
                     /locus_tag="U2M88_001728"
     CDS             3559..4485
                     /locus_tag="U2M88_001728"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEM8374114.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            4667..5560
                     /locus_tag="U2M88_001729"
     CDS             4667..5560
                     /locus_tag="U2M88_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEM8374115.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            5694..5819
                     /locus_tag="U2M88_001730"
     CDS             5694..5819
                     /locus_tag="U2M88_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8374116.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5953..6585)
                     /locus_tag="U2M88_001731"
     CDS             complement(5953..6585)
                     /locus_tag="U2M88_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374117.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(6733..7275)
                     /locus_tag="U2M88_001732"
     CDS             complement(6733..7275)
                     /locus_tag="U2M88_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEM8374118.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            7410..7799
                     /locus_tag="U2M88_001733"
     CDS             7410..7799
                     /locus_tag="U2M88_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374119.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            7803..8567
                     /locus_tag="U2M88_001734"
     CDS             7803..8567
                     /locus_tag="U2M88_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEM8374120.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            8696..9643
                     /locus_tag="U2M88_001735"
     CDS             8696..9643
                     /locus_tag="U2M88_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8374121.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            9773..10513
                     /locus_tag="U2M88_001736"
     CDS             9773..10513
                     /locus_tag="U2M88_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374122.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10804..11472)
                     /locus_tag="U2M88_001737"
     CDS             complement(10804..11472)
                     /locus_tag="U2M88_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEM8374123.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            11606..11893
                     /locus_tag="U2M88_001738"
     CDS             11606..11893
                     /locus_tag="U2M88_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374124.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            12309..13847
                     /locus_tag="U2M88_001739"
     CDS             12309..13847
                     /locus_tag="U2M88_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEM8374125.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            13844..14809
                     /locus_tag="U2M88_001740"
     CDS             13844..14809
                     /locus_tag="U2M88_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8374126.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            14796..16118
                     /locus_tag="U2M88_001741"
     CDS             14796..16118
                     /locus_tag="U2M88_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEM8374127.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      16181..16285
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16359..17102
                     /locus_tag="U2M88_001742"
     CDS             16359..17102
                     /locus_tag="U2M88_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8374128.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            17117..18109
                     /locus_tag="U2M88_001743"
     CDS             17117..18109
                     /locus_tag="U2M88_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM8374129.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            18121..18858
                     /locus_tag="U2M88_001744"
     CDS             18121..18858
                     /locus_tag="U2M88_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8374130.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            18848..19570
                     /locus_tag="U2M88_001745"
     CDS             18848..19570
                     /locus_tag="U2M88_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEM8374131.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(19654..20277)
                     /locus_tag="U2M88_001746"
     CDS             complement(19654..20277)
                     /locus_tag="U2M88_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEM8374132.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            20672..21363
                     /locus_tag="U2M88_001747"
                     /pseudo
     CDS             20672..21363
                     /locus_tag="U2M88_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
     gene            21571..21861
                     /locus_tag="U2M88_001748"
     CDS             21571..21861
                     /locus_tag="U2M88_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374133.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT         7889 a         3799 c         4616 g         6570 t
ORIGIN      
        1 atacaggtgg cgtctctttt tcattcattt ttaataaatc cttatacgtt tgcaaaaaaa
       61 gatgaaagtg ttcacaatgc ttaaatgttg gtatgtcaac gttgatttaa atatatgtaa
      121 ataaaacata aaaattgtta aataaaaaac aagaaaaccc ttgcaaagat agggtgctgt
      181 gttatactaa tcacgtaaaa actgttacat aaaacagaaa tttaggaggt ccatacaatg
      241 gagcaatgga atggatttaa aggtaccaaa tggaaagaag gcgtagacac tagagacttt
      301 attcaaaata actacactga atatcgcggt gatgacagct ttttggaagg cccaacagaa
      361 gcaacagaaa aattatggga tgaattgcaa aagttaaatg atatccaatt cgagcgtaac
      421 ggtgtctatg atatggacac agaaatcgta tcaactatta cttcacacga tgcaggctat
      481 ttggataaag atttggaaaa aatcgtcggt ctacagacag acaaaccttt gaaacaagca
      541 ttcatgccgt tcggcggaat caatatggct aacaatgcgc taaaaagcaa tggctataca
      601 ccaaacgatg aattgacaca tattttcact gactggcgca aaacacataa tcaaggtgta
      661 tttgacgctt atactgctga aatgcgtgct gctcgtaaaa acaaaatcat tacagggctt
      721 ccagacgctt acggtcgcgg acgtatcatc ggtgactatc gtcgtgtggc attgtatgga
      781 atcgactatt tgatggaaca aaagaaaaaa gatcatgata tgactggtta tcatacaatg
      841 agtgaagaag tgattcgttt acgtgaagaa atcactgaac aatatcgtgc attagcgcaa
      901 atgaaagaaa tggctgcgaa atatggctat gatatttcta aaccagctgc aaatgcaaaa
      961 gaagctgtcc aatggctata cttcggatat ttagcagcaa tcaaatcaca aaatggtgct
     1021 gcgatgtcaa tcggacgtat ctcagcattc cttgatatct atatccaacg tgatttggat
     1081 aatggagtaa tcacagaaaa agaagcacaa gaattgatcg accacttagt aatgaaatta
     1141 cgtatggtca aattcgcgcg tacacctgaa tataaccaat tattctcagg attccctatc
     1201 tgggctactt tatccattgc aggtatggga ttagatggtc gttcattagt tacaaagaat
     1261 gatttccgtc tgcttcatac attaacaaat atgggacctt caccagaacc aaacttgact
     1321 gttttgtatt ctgaacacct tccagaagga ttcagaacat atgctagcag aatcgctatc
     1381 gaaagttctt cgattcaatt cgaaaatgat aatcagcttc gtgaacaatg gggttctgat
     1441 gattgtgcga ttgcttgctg tgtatcagga acagttgtcg gaaaagacat gcaattcttt
     1501 ggcgctcgtg ctaacttggc taaaacacta ttatatgcca tcaatggcgg agtcgatgaa
     1561 gtgactggcg cccaagttgg accagaatat cgtccaatca catcagaaat actagacttt
     1621 gaagaagtga aacataactt catgaatatg atggattggt tagcagaact atatgtcaat
     1681 acgttgaaca tcattcatta tatgcatgat aaatattctt atgaagcagc tcagctggct
     1741 ttgatggatt ctgacttgca acgcacattt gctacaggta ttgctgggat ctctcatgcg
     1801 gcagacagct tgtcagcgat caaatatgca aaagtaaaag caatccgtaa tgaaaaagga
     1861 atagcagttg attttgaagt ggatggcgac ttccctaaat acggaaatga tgatgaccgt
     1921 gcagatgaat tagtttcatg gatcttggaa tacttcatgg gacaaatcaa acgtcataaa
     1981 acttaccgca actcaaaacc aacaacttca ttattgacga ttacttcaaa tgttgtttac
     2041 ggtaaaaata caggaaatac tcctgatggc cgtgaagctg gaaaaccact tgcgccagga
     2101 gcaaatccaa gttacaatgc ggaacaaagt gggttattag ctagcctgaa ctcaacagct
     2161 aaaatgaatt acggctgtgc attggatgga atttcaaata cccaaacaat caatccttca
     2221 ggtcttggaa aagaacaaga tacaagaatc gaaaacttgc gtaatgtctt agatggatac
     2281 ttctcaaaag gagcttatca tttgaatgtc aatgtattca gtaatgattt attgcgtgat
     2341 gcaatggaaa atccaatgaa atatccgaac ttgacgatcc gggtatctgg ttatgcagtt
     2401 aaattccgtg acttgacaag agaacaacaa ttagatgtct tgatgagaac ttcacacgac
     2461 agaatgtaaa aagttcgtga aaaaagtttt tcgacttagc gtcgaaagga cgttgaatag
     2521 ccatcgcatc aaaatcgaat aatcagtatg cttatttgaa agaaacgatt tacaggggca
     2581 ggacttgtat gttccgtccc tgtaaatttt acacataacg aagcggcaat tgcgaaaaat
     2641 tttgttcaga aagcttcgct tcgacagcta acttgtactt aaaatgaaat caaagaaaag
     2701 atgtgaactt aaatggaaga aaaaacaatc ggctatgttc attctatcga aactttcggt
     2761 tcagtagacg gacctggact gcgctttgtc gtgtttatgc aaggatgccg catgcgttgc
     2821 caattttgtc acaatccaga tacttggaat attggcggag ggaaagaata cacagcagat
     2881 gaacttttag ataaagcaga acgcttcaga ccttactggg gagataaagg tggtattact
     2941 gttagtggtg gagaacctct attgcagatc gattttttga tcgaactatt taaaaaagca
     3001 aaagagcgga agatgcatac tacattggat acatgcggaa aaccttttac gtatgaagaa
     3061 cctttctttt cacgttttca agaattgatg aagtatacag atttactgtt atttgacatc
     3121 aaacacatcg ataatgaaga gcataaaaaa ctaacgcatt gggacaatga aaatatttta
     3181 gaaatggctc aatatttgtc aaaaatcaac aaaccggttt ggattcgcca tgtactagtt
     3241 cctgagcgaa gtgattatga tgaatatttg atccgactag acaactttat tcaaaccttg
     3301 tcaaatgtag acagagtaga gattcttcct tatcatacga tgggaaaata caagtgggaa
     3361 acacttggtt tgaaatatcc attagaaggg atcgaaccac ctacaaaaga acgtgtcgaa
     3421 aatgctaaac gattgcttca tacagaagag tatacaaaat atttaacgcg ataggtcgca
     3481 tcggaaagaa gataagtatt gcttctttcc gtttttttgc tattattaaa taggagaaat
     3541 cacaaaggag cgaatgacat gtcaaaaatt ttagtttttg gtcaccaaaa tccagataca
     3601 gatgcaatcg gggcagccat tgcgttttca cacttgcaaa atgaattagg taaagaaaca
     3661 gaagcagttg ctctaggtac acctaatgaa gaaacacaat acgcattaga ccactttcag
     3721 ctatctgcac cacgagtagt agagtcagct aaagcagaaa atgcagatgt catgcttgtg
     3781 gatcataatg aattccaaca aagtatttca gatatcagcg aagtaaatat tttagctgta
     3841 gtagaccacc atcgtattgc aaactttgaa acagctaatc cattgtacta tcgtgcagaa
     3901 ccagttggat gtacaagtac gattatttta aaactatata aagaaaacca agtaacaatt
     3961 cctaaagaaa tcgcaggaat catgctctca gctatcattt cagatacatt gttgttcaaa
     4021 tcaccaacat gtacgcctga agatgaagca gctgcaaaag aacttgcagc aatcgctgaa
     4081 gtcgatctaa acacttatgg acttgaaatg ttgaaagcag gaacaaatct aagtgataaa
     4141 tcagctgcgg ttcttcttga tttggatgca aaaagtttcc caatgggcag ttccaacgtt
     4201 cgtatcgctc aagtcaatac agtggacctt aatgaagtga tggatcgaca agcagagcta
     4261 gaagcagcaa tggaagatga aatgaaagca aatggctacg atctgttcgt attagttgtc
     4321 acaaacatct tagacagtga ttcagaatta cttgttgctg gtaaaggaaa agaaaaagta
     4381 gaagaagctt tccaaacaac tttgaccaac aaccgtgctt tgcttaaagg tgtcgtttca
     4441 cgtaaaaaac aagtcgtacc tcaattgacg gctgtattta gctaatcaaa tggtagtatg
     4501 aagcattcaa aatgaatgga taagcgattg atataaaaag aaacatagaa catactaaag
     4561 gccgaagaaa tggaggagaa taacagtcaa ttatgactct tgttcttcct cctttttacg
     4621 taaaagcctt ttaagtaaaa taaaacggat agaaaagaga acatatatga actattattc
     4681 aaaaaataag gaaaactttt atcaaaaact gaccgatgat ccgctttttt cttcattgac
     4741 cgattatcta tatgaacatc gagaacaaga aacgattcta agagaattga aaaaagaatt
     4801 ttcgcaaaat aaattttccc attttcttga tttattgatc gatgcaggat taatcaagag
     4861 ggaagaacga agatatcatt tgaactttcc aatatttgat cctaaagatt acttgcaaca
     4921 agccacttcg gctgctgaaa ccatagctga acaattgaag agactctctg ttgatgaaca
     4981 aaaattagcg atgggagaag ttatttgggc gtattgtttc gaagatgaac ggaaagaagc
     5041 gtatttttat ggagtaagaa acagccgaga gacagaattg ctgaggacaa ctgcaggaaa
     5101 tgagaagtac cgttttatca cactttcctc tatagaacac ttcccgctta ctttggctaa
     5161 ttactttttt gtacagaaga atcagcttcc tgtaacgaag gcattcaaag aattagccga
     5221 acttattgga gacgtaaatg aatcgtactt ttttgatcaa atagaagtta tcgttgacag
     5281 gatacgtaag aataaatata aaaatcgccg gcctagtatt ttccatcagt ctctacttgt
     5341 gacgaatact ataaaggaag aagaatcatt cacattagaa ttacctattg tcgaaaaaaa
     5401 caattttgag attgaattac caacattgga tccttcattg acaatggagg aaatggcctt
     5461 tcttaaacga caaatctttt ctgagctgag caagaaattt ataccgcatg cattttctta
     5521 tatcaaagaa tatggaacag ttttagtgag taaaacctaa gagagcagta agcaaaaatt
     5581 atactgactc ttgcaggagg agcctttctc ttcctttatg gttaaaagaa ttatttgagt
     5641 attttgccta tctaccacct attagattca ctacatggtc tggaaacaaa ggaatgcaaa
     5701 aaaatggtga gaaatgtgga atgacaaagg aagtagtgat aagaaaagtt agatttttaa
     5761 ataatcaata ttatgattca gtcaagtatg gcattttatg ggaagaacta gctgattaga
     5821 ttaggaagtc catttgataa tccagaatca ctaagaaacg aatggcgagt cattaataat
     5881 ttatccaatc ttgggttatt cttttagatt gaagtgttta aaactaaaaa agcatttatg
     5941 aagtcgtaat agtcaggctt caaaatgctt tttattttgt ttgttttttc tttgccacat
     6001 aaatacacct agactgacga tgaagagcat atccaacaga tttcctgcga cttggatcgt
     6061 cgaagggttg gtcagtggca ttaaagcaag gacaaaaaag gaaaacaagc tgtaaataaa
     6121 acaggtgaca ggaactaatc gaaagaagag cggacatttc aattggctaa aaatgagtga
     6181 aaaaataaaa taagtaaaaa tggataaaat cacgactaaa ccattcatga taaagaagaa
     6241 aagcgtattg tgattaaacg aataaaaaag aattgctaaa aaagaaaacg ttttaaatgc
     6301 aatcaatacg cttgaagctt cgtttaacgt accaggatgt cgatgaaaaa aataggcacc
     6361 gaaataaaca gaaaacagta agccgcaaag agaagacaaa atcaaaaaaa acggattttc
     6421 ttcggcttct gtgatatcag aatgatccaa aagcaaccag cctaatgcac tagaaggcgc
     6481 atgtgaggtc ttaaggtatg aataacatag aaaaaataag atcgtcaaag ttgcaaaaaa
     6541 agagcctaaa tgtaaaaaat tagtggttct acgttgaatt tccataatat ctccaaatta
     6601 aattgataag tatataaagt ataataacaa attgatgagt atatgtcgat gttttttaat
     6661 caaaataata aataaaaact gtctaacatt taaaatttaa tttagatatt agacagtttt
     6721 taagtttgtg agttattttc cttgtcgttt tctcactttt ttagtccaga aaccacctga
     6781 aatatatttt ggctcataag aaatgataaa agcacgctct tcgatttctt tgatcaggcg
     6841 atacagttca cgttcgcttt ttctaggaga aagtatttcg agaactagcc gctcaccttc
     6901 cagccccatt gcagaagtct gtgtcacacc ataaccgtgt tctctcagta tacccggcaa
     6961 acttttatct tctgcgctat ttgctggcaa aatcgttgtg accatgatgt atcctaatgc
     7021 caatttgtct tcaatcaaaa ttccgacact gatacctact gcgtaaccta acgcatagac
     7081 aaataaattc agtggattat ctaaacgatc taaaacaagg gataagccga cgatataaat
     7141 cgtgatctcg accatactga caagtggagc aatcagtcga tagcctttca ttgtcaacat
     7201 gaaacgtaac gtgttcagag tgatataagc aaaattaatg aggaagatca tccccaacat
     7261 ttttggatca atcatttgta aaactccttt catttatata acaacaatat caaaaaaaat
     7321 cgtaagtgaa gaaaaattga aactttatac atacaaaaaa atgataaaat gattataata
     7381 gaattataaa gataggttag gtgattaaaa tgaaaacggt caatcgtgtg ttttgtgcca
     7441 gccacgtgct aagtctcctt ctcagtatat acttaggtgt ctattcgatt caacagccaa
     7501 ttgaggtaga gaacattgtt tttcagatgt cgctgacaga tggtttaatt ttaagcgttt
     7561 ttttcttttt gatactgttt attggtaata ttttcggtag cgttgccagt tttttgaatt
     7621 tttctattta tccactcctt tcgctagcat taggagtggt gggactttgt tctctgtgct
     7681 tatttcccga gccattcagt cctgcattta tattatttgg tacactgaac ctgttccaga
     7741 caactgttgg agcatggttg ctatggcgat caggaaattt aatgaaaata ggtgagtaac
     7801 aattgaatcc aattatattg aaacgttctg tccatcatat tccagtgtta gaagttgttc
     7861 tgaaagaatt aagagaggaa cgtattcccg ttgtcatcta ttatcacgga tggcagacaa
     7921 gtaaggagtt agttttgacg caaggaagaa aaattgcgca gcagggcatt cgtgtgatcc
     7981 tgccagatgc gatgaatcat ggggagcgca aacagccggt ttcttctatt ccatcctata
     8041 cattttggga tagtatctac ggaaatctgt ttgaatttga cacacttatc aagcatttag
     8101 aaaaaagagg tcttctacat gatcagcttg cagttggcgg ggtttcgatg gggggaatga
     8161 caacatgcgc cttgttggct aaacatcctc atatagtagc gggcgtgtgt ttgatgggtt
     8221 cccctgcacc tttggaatat ggaaaagaaa tcgtaaggcg cgcatcggaa tataattatg
     8281 tcgtaccaaa agactatttt gatttgattt catgggttaa gagctatgat ttgtcattgc
     8341 agccggaaaa gttagctgga cgtcctctgt tcttttggca tggacaagct gacgaaaaaa
     8401 tcccttatat acaaacggaa cgatttgtag aaaaaaatcg gcataagtct tacggaaaag
     8461 aaattgtttt tcgttcctca aaaacacagc gacatatggt acaaatacca ctaatggaag
     8521 aagcttccac ttttttagca aaaaatttga taaaaaagta taaataattg aaacggaaaa
     8581 tcactcatga cgatagagga ttttccgttt tttattttgg gctataataa actctgttag
     8641 gaaaatttgg tataattgat agtaagaatg acaacatgtt agaaaggaag agagcatgcc
     8701 cactttagca gatgttgcaa aacgtgccaa tgtttcaaaa atgacggtct ctcgagtcat
     8761 caatcatccg gaacaggtga ctgacgaatt gaaagagtta gttttctcag caatggctga
     8821 actggattac cgtccaaata ttgctgcaaa agcacttgtc acgaatcggt cgcagatcat
     8881 caaattattt atactggaag aaatcgacac aactgagccc tattacatga atttgctgat
     8941 gggaatcgct aaacggatcg gtaaagcgca ttattctctc caattagtta caaatgatgc
     9001 cttcgacgtc ggttcttgtg atggatacat catcacaggt gtcagaaaaa aagacttcga
     9061 ttggatcgac cgtttggaaa aaccagttgt cttgtttggg gaaaattctg aaggatatga
     9121 ttatgtagac agcgataatg aatatggtac agcaaaagca acgcaatatg ccttagaaag
     9181 agattatcaa acgattattt atatcggtat cgaagtggac gagccttttg aaatttcaag
     9241 ggaaactggt tatttatctg tgatgaagga agttgatctt cctagtaaaa tcttgcggtt
     9301 tccaaatcat tcgacagaag tagaaagata tatgacagaa aactgggcag attttacaga
     9361 taaaacttgt tttgtatgta gttcagatcg gctagcacta ggagcagtaa gagggattat
     9421 aaatgctgga ggacacttgc ccgaagaatt tggtgtgatt ggatttgatg gggtattttt
     9481 agaccaaatc agttcaccta aattgacaac gatcaagcaa gatattatac gtatgggtga
     9541 agtatgtgga gaaatgttgt taaaaaaaat agaagaaaaa ggggcgagtc aaggttatcg
     9601 tcgttttttt cctgaactag ttatcagaga gacaacgaat taagtcttat tactatatat
     9661 atatttatga aattcatcgc tattttctta aaataatcag cgcattgcta ctgaaaacaa
     9721 tgaatctgtg ctattattga gaaaagttcc atttttgaga aggatggttt gaatgaaaga
     9781 aatcagacga aaacatttcc gttttcctgc gtacgatgat gagttaggcg taaaattaac
     9841 caacgacaag aagcgagtat tatttgacgg acaagaagat cttttgactg aacagaatca
     9901 gtccccgttt gaacagatga aagaatttca tttcacgaac acgaacaccg agaaaatccg
     9961 tcgcaagcgt cgtgcaacaa atggattgaa gtcagctccg cgtaaggaaa atttggaaaa
    10021 gcataaagga aaattaccgg attataccgg ccattaccaa tcagaaatca catcgacagg
    10081 acgaaaaaaa ctttttggtg attcctctcg ttctgtcaga caaagtgaaa caaggacaga
    10141 gtccaagcat actacacgaa acgcatctgt tgacacgaag gtgcgggagc gttcttattt
    10201 tgttcctaaa tatgtacccg catcgattat cccagaccct agagaaagca aaatttctga
    10261 ggatcattta atcgaatcga tgaaaaaaac ggatgactct tatctgttgt tcgatacaga
    10321 accgacgccg tatcaagaaa aaagaaatgg tgagccgtct gtacgaacat tcaatcgaac
    10381 aaaaacgtta gaggaaacaa agcagtcaga agaaaaaaaa tcgaaaaaaa gccatggtat
    10441 cttagaacgc tcattacaag gattgatcga tgatcaagga aactctttgg gagaaaatag
    10501 ctattttaaa taagtgatat acaaatttat attcggctac agttggaaag gaaacgctgc
    10561 ggcgtttcct tattttatac tctatccgaa agtttatatc gtatcctcaa atttttttga
    10621 gtttaagcga attttttctc ccaataagta agaaaataat ggcaaaataa aaaaacaaac
    10681 tattttcgca gatcgtttgc aaaaatagtt tattttttta taaagaagat tttactgaat
    10741 aatatcattt tagtttacat aatgatattt ctgtaaatta aataaagtag tgaaaaataa
    10801 gaatcagtgc tctaactgtt caagaggatc aagtggttca aatgtttctg tagtctgttt
    10861 tgcttccaat tggggcaaaa cagatccaat ttccagatac ttcactttat tcaatgtata
    10921 agatagaata gaacagctaa gtaatgtata cagaaaatgt gtgagatcca aataaagcaa
    10981 ggagacaact aaaataccaa aagtaatcaa gaaaagactt ttgccaatct taatatgaaa
    11041 atatttttca atggcttgat aaatgatatc tgttccgcct gtgcttccgc cgaatcgaaa
    11101 gacgagaccg caaccaatgc cagaaagaat acctgtcatc aaactattga tcagtagact
    11161 atctacagca aaatgtatat gaaggctctc ccataaatgt aaaaagaaaa ctagcgcaaa
    11221 tgttccgacg atcgtccgga gaaaagcgtg ttttccaaaa agaaacaatg agataagcaa
    11281 taaaggtaaa ttaaaaagca gtgaagtgac agaaggagcg aatccaaaaa gattatgcaa
    11341 aagcaaagca ataccagtaa ccccaccatc tgccagctta gatggaatat tgattcctgt
    11401 cacagctaat ccgtagattc ctgcgcctaa aatgattccg cacaattctg taaataaatt
    11461 taattttttc atttctagcc tcttttcttt tgaaaatttt cagcaacttc tttattatac
    11521 tgacttatac tcttacttca agaggaaaat gaaaaaaaat tatttttggc tgtttctttt
    11581 tatttatcaa ataaggagtg gaaaaatgcg agcagcagac ttattgacaa ttcttacaaa
    11641 atcacccaaa aaaacagagt atcaaggagt ttttttggct caagaacaaa aactggtgcc
    11701 tgtgacaatg gtccagataa ataaagcaaa tcaatttatc tttttatttg aacacggcaa
    11761 agcaccactt gctatgaaag acatctttct ttttttgatg aagaaccgtg agaaagctgt
    11821 ttattatcag tcaaaagaag aactcattcc tctttatgga gtgaaagaga tagaaaataa
    11881 agtggtgata taagtaaaaa taagaaaaca agacgtttat gatgtcaaaa caaccatttt
    11941 ttacattttt ggcaaaaaag tgtcatgata cagtataata ttcgttgaac aggccaataa
    12001 aggtgttctc ccccacgaga tttctacttt attggctgtt tctttttatt aaaataaaag
    12061 ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa tttcgtatag atttttatgg
    12121 tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga ctttgttgct tggattcttt
    12181 ttttgtcagc atatttagag gtattttttt cttttttttg ttcgtgctgt aaggttaagg
    12241 caagacaagt tagttgggaa aaaggaaaga aatgtttata cttaaagaat aacagaaagg
    12301 aaggtcgcat gaataatttt atacaaacat taagtgaaag aaaaggcgag ttgctggaag
    12361 ctgcatttca acatctgtcc atttctctca tttctttgct gattgcagca ctgattgcta
    12421 ttcctttagc gatttgggca gccaatcata aaaaaatggc agaagtctta ttgcagatca
    12481 cgggtgtgct ccaaacgatt ccttcacttg ccttattagg tttacttatc ccatttgtag
    12541 ggatcggaac tgttccagca ttgattgcac ttgttattta cgctttgctt ccgattttcc
    12601 aaaacacgta tattggtcta gctgaaattg acccgtctat tgaagaagca gcggtagcat
    12661 ttggtatgtc cagaatgaga cgcttgctga aagtagagct tcccatcgca ttacctgtca
    12721 ttatctcagg tatccgaact gccttagttt taatcatcgg tacggctact ttggcagcat
    12781 tgatcggagc aggtggatta gggacattta ttttgttagg tattgaccga aatacacctg
    12841 ttttgacatt gattggtgcg atcagttctg ccttgcttgc aattatattc agcggactga
    12901 ttcgctggtt gcagcataag aaacctcgat attccatcat tactttagca gcagtggtgt
    12961 gtttatttgg cggcgtttct ttatatcagt ctgccttgtt taaaaatgaa accatcacga
    13021 ttgctggaaa attaggagcg gaacctgaaa tcctgatcca aatgtataaa gaattgattg
    13081 aagacaacac aaaaacagaa gttgaattaa aaccgaattt tggcaagacg agttttttgt
    13141 tcaatgcact ggatcaaggt caaatcgata tctatcctga gttcacaggt actgtcctag
    13201 aaagcttggt gaaggtacct gaagcaatga aaagtgctga tttgacaaaa gaaaaaacat
    13261 atgacgaagc agaagaatta ttgaataagc aattcgatat gacattatta aaaccaatgg
    13321 cttaccaaaa tacgtatgcc ttggcagtca agagatcttt tgcgaacgaa catcaactta
    13381 aaaagatttc cgatttggca gctatctctg atcagatcaa agcgggattc acactggaat
    13441 ttattgatcg ttcagatgga tataaaggaa tccaagaagc atatggcttg aatttttcaa
    13501 gtgtacaaag catggagcca agtttgcgtt accaagcaat caataatgga gatgtcaata
    13561 ttgtggatgc ctattccaca gatagccagc tcaaggagta cgatctagtg actctagaag
    13621 atgatcgagg gttattccca gcttatcaag gcgcaccgct gatgaaaaaa gattttgcta
    13681 aagaacatcc tgaaatcgtt aaagcactga ataaattaag cggaaaaata acagaggatc
    13741 aaatgattga tatgaattac caagtcaatg tcgaaaagaa aaagccgaca gaagtagcac
    13801 gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga tcagtgacta aattaatcga
    13861 gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc atcgatgacc taaatctggc
    13921 aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc ggtagtggaa aaacaactac
    13981 tttgaaaatg atcaatggat tatctaaacc atcagcagga gacatctact tcaaaggaaa
    14041 atcgttaaat gaatataatc tgcagaaaat gcggtggaat atgggatatg tgctgcagca
    14101 aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt gaagtcatcc ctgaaatgtt
    14161 aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa cttcttcaaa aagttggctt
    14221 atctcctgat atttatcgag atcgtatgcc tagagagttg tctggaggag aacagcaacg
    14281 gatcgggatc atccgagcta ttgctgcaag cccagatgtg attttgatgg acgagccatt
    14341 tagcgcatta gatccgattt caagaaactc attacaagaa ttggttttgt ccttacacga
    14401 agaattgggc acgaccatcg ttttcgttac gcacaatatg gaagaagcaa tcaagctagg
    14461 tgatcgtatt gcatttatga aggatggaga aattatccaa tgtgacacac cagagcagtt
    14521 attgatgaat cctaaaaacg actacgttcg gcatttcttt gatgaaccga aacagacgaa
    14581 agagtggcga gtagaagatt tagtcgtcaa tggatatttc ttaaatgaaa taccagaaaa
    14641 tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa gtatattcgc ttctttcggt
    14701 gtatccaagc attgtgatag tagaaaagca aagaagtatt gggtcgctga caagtaaaga
    14761 aatctttgct tttttaagtc gagaggagga aggaaatgaa aacatatgat gcaatcatca
    14821 tcggttcagg tgtgagcggc ttaagtgctg cttatggatt gaaagaagca ggtaaaacag
    14881 tactagtggt cgaagaagat ctatggggcg ggacttgtcc taaccgtgga tgtgatccta
    14941 aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt aaaacaattg agtggaaaag
    15001 gttttaacga gatcccaact gccaactggg aagaacttca aaaattcaaa cgaacattta
    15061 cggatccagt cccagaaagc cgaaaaaaac aattagcaga agctgaaatc gatcatcttt
    15121 caggaaccgc tcgttttttg gatgattcat ctattgaagt caacgaagaa gtcttccatg
    15181 cggattactt agttttggca accgggcaaa gacctacgat cttaccagtg gagggaaagg
    15241 aatacttgaa aactagtgca gactttttat cattgcctgt actgcctaaa gaaatcattt
    15301 ttatcggtgg tggttatatt gcttttgagc tagctacgat tgccaatgct gcagggagca
    15361 aagtaaccat tgtccatcat aatcaacggc cattaaaaga atttgaggcg tctttggtag
    15421 aggaagccgt ccatcaaatg gaagcatctg gcattcagtt tgcttttggt gttgaaaccc
    15481 aaaagatcat ttcagaaggt actcgctatc gtttagtcgg aaaagagacg gaattagtag
    15541 cagatatgat tttttgtgcg actgggagac agccgaatac agaatcttta gcacttgagc
    15601 aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa tgattatctg caaacgagca
    15661 atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa aaccccaaaa ttgacaccag
    15721 tcgccacttt tgaaggaaat tatgtagcta agcggataac ggatgcaaca agcgaaccta
    15781 tcaaatatcc gattatacct acgatcgtct acgccagtcc taaacttgct gaagtaggag
    15841 ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga gatggatctt accagttggt
    15901 tcacctatca tcgtgtcaat gaaccagttg ctaaggctga attgaccttt gaccagcaaa
    15961 attatttgat tggtgctgca gtgattagcg agcaagcaga cgaattgatc gatgatttga
    16021 cactcgtcat caatcaaaaa ttgactaaaa aagagttgga tagctatatc atggggtatc
    16081 cgacattggc aagtgatctc tcctatttat tgaaataatc agcatgataa ctaaagcgct
    16141 tgttgctttt tccttcttgt gctacacttt aaaaaatgaa tacctgtttc tggggatgtc
    16201 gtagacgaca tgagagtaag ttgtgaactt agacccattg aacctgtcag ttaatactgg
    16261 tgaagggaga aacatagcag acgcttgaag cgggttacta tgtcgtgccc atttcaagcg
    16321 ttttttgtta ttcatattag aagaaaggag gtaacaaaat gaagaaaaat ctttatccag
    16381 cagtcagtat ggcattcatt ttaattattt ggcagattgt tgtcatagtt tggaaaatac
    16441 ctgcctttat tttacccgga cctgctgctg tattttctgc attagcggca gatgcacaag
    16501 tattactttc ccattctgtc acgacattaa atgaagcagt gcttggtttg gtaatagcta
    16561 gcgttctttc ttttattact gccttggcaa tggattattg gaagttttta gaaatgtctt
    16621 tttatccgtt actagtgatt tcacaaatgt tgccaatcat ggtgttaggg ccattactta
    16681 ccctatggtt tggtttcggt atgcttccaa aagtgatcct tgttgtcttg ataagctatt
    16741 ttccgatcgt agtttctttt tcggatacat tgaggaaagt atcaaaagag caaatcatct
    16801 ttttgaaaac gatgggggca gatacaggga agatttatcg aatatataaa ataccagcgg
    16861 gtttatccgg cttcttttcc ggtttaaaag tggctgctac ttattgtgtc agcggagcag
    16921 tcgttggaga atggctaagc gctcaagcag gcttagggta ttacatgata cgcgtaaaaa
    16981 acagctacca gatcgataaa gtttttgctg cgatcatttg tgtcattcta ttgagtttat
    17041 tgctgaatgg cgcttgttca ttattgaaaa aagggtatta tcgtatgctt tatatttttt
    17101 aagagaaggg gatcaaatga aaaaattatg gcttttatta ccgctactgt tgcttttatc
    17161 cgcttgtggc actgcaaaag aaacaagtag caaacaagaa ataaaggatt tgaaaaaagt
    17221 gacacttgtt ttggattatg taccgaatac taatcatact ggcatctacc tagcaaaaga
    17281 aaaaggctat tataaagagg ctggtttaaa tgtccaaatc atcgaaccag gagataattc
    17341 gacaagtatt ggtctagtag gtgcggacaa agcacaattt ggtgtaagtt atcaagaaga
    17401 tgtcacttat gctcacgcag atggacagaa tataccagtc aaagcaattg ctacagtgat
    17461 caaacataat acttctggat tcgccaccct atcagacagt aacatccatt caccaaaaga
    17521 ttttgaagga aaaacatatg caggttggca aagtccaagt gaagaagctg tcttaaaagc
    17581 tgtgatggaa aaagacggcg gggacttttc aaaactgaca atggtaggaa gcaatggaga
    17641 agggccagaa agcttaggaa aatcctcaga tatccaatgg tactttgaag gttgggatat
    17701 gatcaaagca aaagaagcgg gtattgaact aaactatatc ccattgaaag aattagatga
    17761 acggttagat tactatactc cggtaattat tacgaatgat caactgatca aaagtgatcc
    17821 agaattggta cagtctttta tggatgccac gaaaaaaggc tatcaggaag ctattaagga
    17881 tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa gaaaatgatc ggacgttttt
    17941 agaagaatca caagcattct tgtcaaaaaa ttatacggat gatcctgaga attggggatt
    18001 gatggaagaa aaagtttggc gcaattatac agcttttatg caggaaaacg gattgatcaa
    18061 gcaagatgta ccttcgcaag aactattcac taatcagttt ataaaatagg agtgatagat
    18121 atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa acctgaaaat attagaagac
    18181 atccatttca ctatccctaa agataccttt acatcgattg tcgcgccaag cggagccggt
    18241 aagtcaacat tgctgaaaac tttaacagga gtccagccta tgacttctgg aacgatcaaa
    18301 gtagacgatc aaaaagtaac tggtctccat acagcattta gttatatgcc gcaagaagat
    18361 atgttacttc cttggctgag cgtctatcaa aacgttacgt tatatcaaaa aatcaatcat
    18421 ctaacaatca atgaagaaca agtaagggat tatctggata tctttgggtt aaacggctat
    18481 gaacattttt tgccggaaca attatctggc gggatgaagc agcgaacggc cttgttacga
    18541 acaatcatga atccttcttc ttacttgctt cttgatgaac catttggagc gttagatgct
    18601 atgaccagag ggcagatgca agactggatg ttgaagctgc ctaaaaaagc aaagcggact
    18661 actttacttg tgacgcatga tatcgaagaa gcaatttatt tatcagacag gattcttgtg
    18721 ctttccgccc gtccggcaca tgtgattgct gagattcaag tgcctgaaaa acgacggtca
    18781 cgtgaatggc tactgcagca atcagagttg aagcaaacga tttatcagtt attggcaggt
    18841 gaattagatg ttaagtgatg ctcattgtca tttagatggg agtcagagtt tagcactcct
    18901 tcagcaggaa cacacgattt tgacgatcat caattgtgat tcgccagaag aatggaagga
    18961 aaacagacag cttgcagcaa gtaagactca agccttaagt tacggtatcc atccttggaa
    19021 agcagatagc tatacgtttg aacaagttga gccttttttg aaaaaagcaa ggatcatcgg
    19081 tgaaataggg ttagataata tttggacgaa tgtaccgatg actacacaaa aaaaagtatt
    19141 tgagcgacag cttgcttttg cagccataaa tgagaaacct gttgtactgc acacaaaagg
    19201 atgcgaaaaa gagatcttaa attatatcca agcttaccct aatcactatt tgatccattg
    19261 gtattcttct ttggactatc aacaagacta tattgattta ggctgttatt tcagcattgg
    19321 tcttgatttg aaaaagaatc cagcagtctg gcaactagct aaagcagtcc caatcgacca
    19381 cttacttatt gaaacggatg gactacatgc agcagagtgg gctttagggg ggattttttc
    19441 agaaaaagat tatgtctcac tgcttgaaga acagttaaag acaatcgctg agatcaaaga
    19501 gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac ttggaacgtt tcattagatt
    19561 aggtaaataa aaaagagatg tgacacaact agtgtcgcac ctctttttgg ctttctttgt
    19621 ataaaagtaa actgtttgat taataaatga atcttagtac caaccgtttg cttcccagaa
    19681 tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg ctactctttc
    19741 ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt aacgtccgat
    19801 gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga tccattcttt
    19861 tgcagagctt gtgcttgctg gagtagcagc agtttctgtt acagcaggag caggttgtgt
    19921 ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt tcttgtacaa caggttgttc
    19981 agcttggact ggtgcttctt gaacggctgc ttgaggttca gcagctgctt gtgcagagcc
    20041 ttctgttggg attgttaatt gttgtcctga gtaaatcagg ttaatatcag aaattgaatt
    20101 tgattctgca atggcattga tcaaggagtt atcaccaacg tatttttgag aaatcgttga
    20161 tagagtatca cctgattgaa cggtataagc ttcgtccgca tgagcagtcg ttcccatgaa
    20221 gaatgcagcg ccggcagcca aagttgttcc aaaaagtaaa gttttaagtg atgtcatgaa
    20281 aaatctcctt tattcgttaa aaagttttat ttgttgtgtt caacgaggaa tactttaaca
    20341 cgtgaaaata tttcataggt aaaagaagca tgacaattta tgacaaatcc gcgttttttg
    20401 taataaatac agtaatctat gaaatgaaaa gggttacgtt taataaaact tgcttttttg
    20461 tttttgttca ccataaatat ttatttaaga aagtattaac ctgcattttt ctctataaaa
    20521 aagatttttt aaatagataa aaaaggagga ttaaatgagt atttgatagt atttatatta
    20581 aaattacata tgtaatttaa aaacgcttga cattgtgttt gagatcactt acttttagca
    20641 tgtaaaagta ttggattaag gagctatgca aatgaaaaag aaatttatta ctggtgggat
    20701 tgtcgttgca tttctatgtc taattggcta cacaggcacg cggtacgcaa atgaaattat
    20761 tttgtggggt ggagagacag atattgatac aatcaatgag aatctgacac agcttgatca
    20821 tacattgaca gataaagagc aagcgattac agatcttacc gctcgactag cacaaaaaga
    20881 acagacattg caagattcaa aggcagatgt ccaagaatac caaacaaaag taaatgaatt
    20941 agaaaaccaa aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc aattagtcag
    21001 caaggatgca caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa
    21061 ccaaaaaaat gatgaaatca atcatctgaa tgcagattgg acagcaagac tacaagaagc
    21121 acaagcaaaa ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac
    21181 actgacacaa gaaaaaacaa atgtagaaat cagttaacag atacacaaaa gaaattagac
    21241 gaagcaactg ctgaaaactc aagcttgaaa caatatattc aaaagttaga aaaagcaaaa
    21301 gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca tttgcgtaaa
    21361 taatctttct taacaagtaa acctaaatta gtaaagaagc aacagtaatc tatactgctg
    21421 cctctttttt ttagtacatg tccttcatct tcgcaggaag tgccaatatg attttaagaa
    21481 aggtaataga caaaaagtca aaaagataaa acagagtttc ttctttacaa agggaatgta
    21541 tgttcgtata atggtattga ggtgaaggtt atggagaaaa aatatggaac agtagataag
    21601 atccgtactt tgcaagttag tgccagaccg ttgatccgtt tcagtttaaa tggggtcaat
    21661 tgtttgattg cttcccatag tttgaacttc ctagctgaag tagacgaagg gatgaagtta
    21721 gtcgttacag gttattataa taatagaaag caatttgttg tgagatccta tcatgtgtta
    21781 gggaaaccca aaattgtggt agaatatgag aaaagtctat acccaagaaa aaaggtccga
    21841 caagatgaaa gaataaacta gaaaacaagc ggctgagaca aaaatgtcct agccgcttgt
    21901 tttggaaaga tggatgcaga cgatgggatt tgaacccaca cgagcatacg ctcacaagaa
    21961 cctcaatcta tatataacta catgtattta agtatttgtg atgaatcact aaactttcca
    22021 aactcatttg agggatattg cttaaagatt gaacagatca attcgaagcc ctattagtat
    22081 tcgctcaatg gggggatcag aggccataaa aagagactag tcattactag tctcaggtag
    22141 tggaattgta ggattttaaa cgttcctgga gagtaccggt atgtaactcg aagaggtgat
    22201 tatcatagtc gtaaaagtaa attgaagaag cctcgccttt cacacgagaa cgtcctggtt
    22261 cgacagttaa acctaacatt tggatttttg agacaaacga atctatatct tgttcgtcaa
    22321 ttttaaaagc aatatgattg tatgtctttg gcagtttatt cgacgaattc tgcattactg
    22381 caatccatag gtccttaact aagaaaaatt tttctggata taatgaaaat ttttttgttg
    22441 actatcgtag atttcttttg catcaaataa ggtatttaac aattaagtat ttaaaagata
    22501 acttttaagt tatctttaag taatgcttaa agctatgtta tactctatta ggaaatataa
    22561 gaaggagtat aaaaatgaac aaacataata gaaaaataac gaataacaaa gaactgatct
    22621 tgttactaat agggatttgg ggtatctttt tactctttaa tatgtatatg ccgactatga
    22681 gagcggatga cgtagtttat gcaagtagat tagatgaact tggctatttg ggtgcttcga
    22741 tagaacatta taagacatgg agttcaagaa ttattataga actattctta atgttctttt
    22801 ctaaacactt tatgctatgg aaattattaa attctacgat tatgttagga attgtagtat
    22861 tactttgcaa gtat
//
LOCUS       DASHRQ010000041        22857 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.041, whole genome shotgun sequence.
ACCESSION   DASHRQ010000041 DASHRQ010000000
VERSION     DASHRQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.041"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(104..1336)
                     /locus_tag="U2M88_001749"
     CDS             complement(104..1336)
                     /locus_tag="U2M88_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEM8374134.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="U2M88_001750"
     CDS             1591..2160
                     /locus_tag="U2M88_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM8374135.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="U2M88_001751"
     CDS             complement(2338..2778)
                     /locus_tag="U2M88_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEM8374136.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="U2M88_001752"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="U2M88_001752"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEM8374137.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="U2M88_001753"
     CDS             3732..4655
                     /locus_tag="U2M88_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEM8374138.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="U2M88_001754"
     CDS             4731..5870
                     /locus_tag="U2M88_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEM8374139.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGCLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="U2M88_001755"
     CDS             5863..6663
                     /locus_tag="U2M88_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEM8374140.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="U2M88_001756"
     CDS             6663..7490
                     /locus_tag="U2M88_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEM8374141.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="U2M88_001757"
     CDS             7468..8202
                     /locus_tag="U2M88_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM8374142.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="U2M88_001758"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="U2M88_001758"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEM8374143.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="U2M88_001759"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="U2M88_001759"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEM8374144.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="U2M88_001760"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="U2M88_001760"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEM8374145.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="U2M88_001761"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="U2M88_001761"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEM8374146.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="U2M88_001762"
     CDS             11787..13820
                     /locus_tag="U2M88_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374147.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15143
                     /locus_tag="U2M88_001763"
                     /pseudo
     CDS             13864..15143
                     /locus_tag="U2M88_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            15353..16159
                     /locus_tag="U2M88_001764"
     CDS             15353..16159
                     /locus_tag="U2M88_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8374148.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16171..17391
                     /locus_tag="U2M88_001765"
     CDS             16171..17391
                     /locus_tag="U2M88_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8374149.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17381..18967
                     /locus_tag="U2M88_001766"
     CDS             17381..18967
                     /locus_tag="U2M88_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEM8374150.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19006..21144
                     /locus_tag="U2M88_001767"
     CDS             19006..21144
                     /locus_tag="U2M88_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM8374151.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21512..22495)
                     /locus_tag="U2M88_001768"
     CDS             complement(21512..22495)
                     /locus_tag="U2M88_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEM8374152.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         7176 a         3899 c         4710 g         7072 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag ttgggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctgtcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcacagcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt tatcgtgtat
    14281 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
    14341 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
    14401 gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg
    14461 ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
    14521 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
    14581 ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
    14641 aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag
    14701 gagaaaaatc tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc
    14761 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
    14821 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
    14881 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
    14941 tggaggatac attgacttca taaccatggg ttccgattat ttatatcctg ttggctatgg
    15001 ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat
    15061 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
    15121 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
    15181 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
    15241 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
    15301 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
    15361 agatttgtat ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc
    15421 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
    15481 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
    15541 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
    15601 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
    15661 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
    15721 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
    15781 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
    15841 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
    15901 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
    15961 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
    16021 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
    16081 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
    16141 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
    16201 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
    16261 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
    16321 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
    16381 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
    16441 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
    16501 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
    16561 atcattgaat ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc
    16621 gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt
    16681 gtggatgaag cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt
    16741 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt
    16801 cgtcgttttt gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat
    16861 acgatggaag taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg
    16921 gatcggaata aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa
    16981 gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta
    17041 tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt
    17101 gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc
    17161 aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg
    17221 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
    17281 gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
    17341 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
    17401 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
    17461 agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca
    17521 atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc
    17581 tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca
    17641 gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac
    17701 attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa
    17761 cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta
    17821 tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
    17881 tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg taggtgatac
    17941 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg
    18001 accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag
    18061 ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac
    18121 gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac
    18181 actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc
    18241 tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa
    18301 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt
    18361 cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg
    18421 ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac
    18481 aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga
    18541 aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga
    18601 aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa
    18661 aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa
    18721 aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa
    18781 gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa
    18841 tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga
    18901 gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga
    18961 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
    19021 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
    19081 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
    19141 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
    19201 gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc
    19261 ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac
    19321 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
    19381 ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg
    19441 aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa
    19501 gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct
    19561 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg
    19621 tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
    19681 tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat
    19741 ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca
    19801 ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta
    19861 aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc
    19921 aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat
    19981 tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt
    20041 aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct
    20101 gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca
    20161 actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca
    20221 gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt
    20281 tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag
    20341 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca
    20401 aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt
    20461 gcagaataca agcatcagct taaagaccgc tttatcgtag aattgattga cattccattc
    20521 aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc
    20581 ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca
    20641 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
    20701 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
    20761 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
    20821 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
    20881 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
    20941 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
    21001 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
    21061 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc
    21121 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
    21181 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
    21241 ataagtcgag gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa
    21301 ttgcttttca aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac
    21361 cgtttattcg tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta
    21421 ttcatgctca gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg
    21481 tcaaagaatc gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt
    21541 cctctacaaa agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa
    21601 ccagattgtt cttagcgtca atataaagaa catttccact attaccaatt gctgaatata
    21661 tttcgttctc agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt
    21721 ctaaagcact ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt
    21781 ttcctttata catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg
    21841 ataaacacaa accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg
    21901 ttttaggaga cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat
    21961 tgacatggtt tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg
    22021 ttagcttact tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt
    22081 tgaattcgcc ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat
    22141 cttcacttga acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg
    22201 ttagcaagtg cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact
    22261 ctggaaaata atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa
    22321 tgccaatcag caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt
    22381 ttttataacc attccattca tctgtataaa cttctacccc atccttatat gcgacaattt
    22441 gacatatatt tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat
    22501 attacctcac tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat
    22561 tcttcacgta atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct
    22621 gaaaaatata aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct
    22681 ttaatttacc aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg
    22741 tcttgattag aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc
    22801 ttataaacta ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgtg
//
LOCUS       DASHRQ010000042        22325 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.042, whole genome shotgun sequence.
ACCESSION   DASHRQ010000042 DASHRQ010000000
VERSION     DASHRQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.042"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            5..403
                     /locus_tag="U2M88_001769"
     CDS             5..403
                     /locus_tag="U2M88_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEM8374153.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="U2M88_001770"
     CDS             387..1082
                     /locus_tag="U2M88_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEM8374154.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1676..2563)
                     /locus_tag="U2M88_001771"
     CDS             complement(1676..2563)
                     /locus_tag="U2M88_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEM8374155.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2748..3587
                     /locus_tag="U2M88_001772"
     CDS             2748..3587
                     /locus_tag="U2M88_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEM8374156.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3783..4757
                     /gene="sgrA"
                     /locus_tag="U2M88_001773"
     CDS             3783..4757
                     /gene="sgrA"
                     /locus_tag="U2M88_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEM8374157.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5055..5411
                     /locus_tag="U2M88_001774"
     CDS             5055..5411
                     /locus_tag="U2M88_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374158.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5414..6571
                     /locus_tag="U2M88_001775"
     CDS             5414..6571
                     /locus_tag="U2M88_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8374159.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6597..7037)
                     /locus_tag="U2M88_001776"
     CDS             complement(6597..7037)
                     /locus_tag="U2M88_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEM8374160.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7197..7703
                     /locus_tag="U2M88_001777"
     CDS             7197..7703
                     /locus_tag="U2M88_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEM8374161.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7871..9783
                     /locus_tag="U2M88_001778"
                     /pseudo
     CDS             7871..9783
                     /locus_tag="U2M88_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
     gene            9788..10789
                     /locus_tag="U2M88_001779"
     CDS             9788..10789
                     /locus_tag="U2M88_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM8374162.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10991..11197)
                     /locus_tag="U2M88_001780"
     CDS             complement(10991..11197)
                     /locus_tag="U2M88_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEM8374163.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11843..12691)
                     /locus_tag="U2M88_001781"
     CDS             complement(11843..12691)
                     /locus_tag="U2M88_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEM8374164.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12690..13757
                     /gene="gnd"
                     /locus_tag="U2M88_001782"
     CDS             12690..13757
                     /gene="gnd"
                     /locus_tag="U2M88_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEM8374165.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            13759..14760
                     /locus_tag="U2M88_001783"
     CDS             13759..14760
                     /locus_tag="U2M88_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEM8374166.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14757..15041
                     /locus_tag="U2M88_001784"
     CDS             14757..15041
                     /locus_tag="U2M88_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEM8374167.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15074..15871
                     /locus_tag="U2M88_001785"
     CDS             15074..15871
                     /locus_tag="U2M88_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEM8374168.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15885..16718
                     /locus_tag="U2M88_001786"
     CDS             15885..16718
                     /locus_tag="U2M88_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM8374169.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16736..17593
                     /locus_tag="U2M88_001787"
     CDS             16736..17593
                     /locus_tag="U2M88_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEM8374170.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17615..18124
                     /locus_tag="U2M88_001788"
     CDS             17615..18124
                     /locus_tag="U2M88_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8374171.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18161..18562
                     /locus_tag="U2M88_001789"
     CDS             18161..18562
                     /locus_tag="U2M88_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374172.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18674..19027
                     /locus_tag="U2M88_001790"
     CDS             18674..19027
                     /locus_tag="U2M88_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374173.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19061..19504
                     /locus_tag="U2M88_001791"
     CDS             19061..19504
                     /locus_tag="U2M88_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEM8374174.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19521..20606
                     /gene="uxuA"
                     /locus_tag="U2M88_001792"
     CDS             19521..20606
                     /gene="uxuA"
                     /locus_tag="U2M88_001792"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEM8374175.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20599..21312
                     /locus_tag="U2M88_001793"
     CDS             20599..21312
                     /locus_tag="U2M88_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEM8374176.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21519..21668)
                     /locus_tag="U2M88_001794"
     CDS             complement(21519..21668)
                     /locus_tag="U2M88_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374177.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22064..22264
                     /locus_tag="U2M88_001795"
     CDS             22064..22264
                     /locus_tag="U2M88_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEM8374178.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7688 a         3430 c         4625 g         6582 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa
     1501 aaaacttttt taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca
     1561 aataaaaaaa ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga
     1621 atttccaaaa atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt
     1681 tgaatatatt ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac
     1741 ggattgtttt atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta
     1801 gctcagattt gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta
     1861 gataatagct ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc
     1921 cgttttttag tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat
     1981 caaaattcat ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa
     2041 gcttggagaa attttctcct tcatttgctt ctttttttgc tttatctgct tcttctttag
     2101 tcgaaaatag aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta
     2161 gatctttatc tgttaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt
     2221 taaagagttt cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt
     2281 ttggaccgag ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct
     2341 cttttccgta tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat
     2401 tatttgattt aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa
     2461 acgttgcaat cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg
     2521 aaaggcacat aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa
     2581 cagtctaaca gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt
     2641 cttagttcgt aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga
     2701 caagaaagaa aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta
     2761 ttcggttgat tggatctgtt ttaatagtct tagttggttt tgttttagga attcttgtca
     2821 ataatgcact tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta
     2881 cggtcggaca tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa
     2941 aaagaataga agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag
     3001 gtctcgttgt tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg
     3061 cgtatcggtt agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat
     3121 ttattgcagt gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc
     3181 ttagatttgg tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg
     3241 gattgaatcc taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt
     3301 tcagcgcact tatctacagt acaggaagtt tatttacggc tattggtgct catattactt
     3361 ggaattggac acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt
     3421 ttttctccac tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg
     3481 aagcatctat atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg
     3541 catataaaac aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag
     3601 caaggctgat atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca
     3661 aattcaagcg aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca
     3721 tcattttcat tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag
     3781 aaatgaagaa aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa
     3841 tgattggaat cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga
     3901 aaactgatcg gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa
     3961 acaaaagcgt gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg
     4021 gaagttcaga aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta
     4081 ttgagtatac aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata
     4141 aagttgattt gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa
     4201 gcagttggac agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta
     4261 cagaaaacac gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa
     4321 acacgagcag cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcacga
     4381 gcagcgaaag cagtacggag agtacgagta gtgaaagtag cacaggaagc atgagtagtg
     4441 agagtagcac aggaagcacg agcagcgaaa gcagcacagg aaaagtaaat aatgaacttg
     4501 atttggtacc tatcatacca actaaggaac aaaagagaaa tgcagggagc aacctaaaaa
     4561 gtgtgctaaa acagcctgat tttgtttctc gtggaattga tcatcaagaa aaagcgaaag
     4621 aaacagataa tagaaaaagc gatttaccag aaactggtag tagaaccttg aacaagctaa
     4681 ttacatggat gggtgtgtta ctcattttga ttgtaggagc aagctatttt cgttctctct
     4741 tccatcgagt gaaataaaga ataaaagcaa cagatctaaa attgaataaa acgatagatt
     4801 tttgagtgaa tatttcattc gagagtgctg tattgcctgg cgaagttgta gctttgccag
     4861 atctattttt gtttactaac atatttgaga aacaggaaat aacaggaaca caaatggaaa
     4921 ataagagcaa gaataattga caatgcacac tgcgcatggt agtatctctg aaaaatcagt
     4981 aacgaatcta tttgtacgtt attcatgtag ctgtcggaat aagtctaaat aatagataaa
     5041 aaggagtatg tactttgttt gaagaattta ttgatatcaa tgaaaggcaa gtatatcaat
     5101 ttttgaacta ttgttatgaa agagacgaga aattatatgt ggtgaaggat atcgctttag
     5161 atctaaacta caccctggct aagatgaatt cggtgattca acaagcagaa tcgttttgcg
     5221 aaaggtatcc agaatataaa ttatcttttc tttcagaaaa caagatgata aaagtcgagt
     5281 tttcttctca gtttttactg agtaaagttt attctatact tttagaggga acgattggtt
     5341 atattttact ggattcactt tacaaaggta cttatcaatc cttagaaaat cttagtcaaa
     5401 aaattatata agtttgcgta cgattcaaag aaaattaaga gaacttaata ctattttgaa
     5461 aaactatcat ttatcttgca gcttgaaaaa aggtaatccg atagaaggag aagaataccg
     5521 tatccgatac ttttttcact tgatgtattg gcagtcattc gacgaaaatc attctgatat
     5581 attatcaagt ataaaaaata aacgtcaact attagaaaaa tttgcgaaac atgcgccata
     5641 tacgcgtagt atagatagaa agaaatggat cagcctacta agaatttcgt tgcaacgaat
     5701 gaaacaaggc cactcgatta cgtatgtccc agaagagatg aacaaatttg tgcatccatt
     5761 gataacaaaa gaagagttta cgcattacat actagagcct ttttttgaag caaacttctg
     5821 gtcgatgaat cacatcactc caacagagca agcttacctt tattttatgt ttggtgtaat
     5881 gaacacctat ttggaagaag atttgttgga tactccagat aaagtacaag aaagttgggg
     5941 ctggattgaa tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt gttttacaac
     6001 ttcagaaaaa aattatctat atgtcaattt ggcaatgatc catttgtatt caagggtttt
     6061 tgggacaaaa aagaaggtgg atgcttttgg aaaatcagca gaagacagag attttcagca
     6121 tatatttcca gtgatgtatc caatcatgca acagtttttt agtggggtag ctcgcagaat
     6181 tccagagctt aatacttatt atgaaaagaa cagacggttg atttttcaat actgtatgtt
     6241 agtcagggat atttttataa aacacgaaca acctgtcata ttctatattc aatcaaagta
     6301 tggtaaaaca caggaattat gggcaaagaa aaggatatta tctatgactg aacgcccaat
     6361 aaaatatagt gcatccgctg atcagttacc tgatattgtc attagcgatt atcctatcga
     6421 caaaaaggca tatgatcaaa cgaatacaca gatattttac tggaatggac aacctacaaa
     6481 aaatgattgg gcgcgtctga aagctgaaat cataaaaatc aggaatgaga aaaatagatc
     6541 catattggaa aaattggatt tcgttaatta agaaaagcag attgtttatg ccaaaattaa
     6601 gacattgcaa aaagattatt ttctttaaaa tcgacaagat gaccagaaag caagagttcg
     6661 ttgagctctt gctttctttt agttttagaa aggaacatcc tgattttatc ttcgctaaat
     6721 ccagtcaaaa gttttgcaga ttggtcgtag ataatcggtt ttttcaataa agaaggggat
     6781 gtacgaatct caatatatag ttcactcaaa ctgagttcat ctattctgtg aacaagattt
     6841 ttataactaa tagaatgagt agagagcatt tcggtcacgc cgttgtttgt caattttagt
     6901 aagtggataa attcattttt ggttagtggt tgacgaatta agttctgttc gataaacggt
     6961 atatcatgga cgataagcca acgttttgcg cgtcttgaag acgagcagcc agaacctgta
     7021 tacaatttaa tcatcataca ttcctccaat agctaagtat ttatgaaata ataacatgaa
     7081 gaaatagtcg aaaagtgcca tttagaggtg taaaataaga aaatgttgtc acgtataatg
     7141 gaactagcta gataagaaat actattaaat caagaaaaaa gaaagaaggc acaagtatgt
     7201 caaacacagc cgacgcaata gtagtgatcg atttacaaaa aggggtatgt tttgattctt
     7261 cgccgataca cgaattagaa acagtgacag cattagtgaa taaaagaatc aaaacatacg
     7321 aaacgattgg gaaaccagtt attttcgttc aacacgaaga tgctcaatta gtgccacaaa
     7381 caaaaccatg ggagctattg tcggaattat ctattcctga gcacagttat tttgtgagaa
     7441 aaacccatgc gaattcattt tataagacta atttgagtgt tttattaact gaattggact
     7501 gtcattcgat cgaattatgt ggggcacaag cagagtattg tatagatacg actgtgaagt
     7561 ttgctcatgg attaggatac cagcttatga tgtgtagagg agcaacgaca acttatgata
     7621 atccttttat gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg gataaacgat
     7681 ttctgacatt tttggagcaa taagttgttt cttcgtttaa aaaaagcaga atggatactg
     7741 gtttatccat tctgctttat tacgtaaaat gagaaaataa tcctgttcta tttttatggg
     7801 ataaaccatt tattttttgt ataaaaaatg tttgcgttta catataattc tcctagaaac
     7861 ataaaaagga gtgaatagct tgagaacaaa acaagtatta aagggagcgt tggtcatctt
     7921 atttgctgta agtacaagcg tcccgcaatg gacagtatat ggagagagca tttcttccga
     7981 atcggaatct cgggaggtag aaaccacaga aactactgat gctggaaatg aaacgaaatc
     8041 gagtacttca caaacagaaa caaatactga aacaacggtt gagacatctg ttactagcac
     8101 agatcaagaa acgaccgcta ccacagataa ccaacaaaca gaagaagcaa cagctacaaa
     8161 aatgtcaaac gacgaatcct cttctgctaa agtaagtgac aaagagaatg ggacagaaaa
     8221 tgatgatatc ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa
     8281 acagatgaca gaaatactta atggaccaac agaagacatc aagtcaaaat taatagagtt
     8341 attagtcact gagcttgctg ttaatgatta tggtgtagat aaagagatta tcaaacatct
     8401 gattgctact tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat
     8461 gcttggaggt acagatgatg ggacaacgcc tacagaaaac acaggcttct atccaaatac
     8521 aaaacaactt catcaaactg tactaaacaa acaagatggc aaaatgatgg atgtatatgc
     8581 atattatgtc gatcaaggtt cagataaaac agtcatgatc catggcggat ttcgagggaa
     8641 ttggaataac gggatcgtta cagaagaata caatgatttt tataaggccg ggtataattt
     8701 attatttgtt gattccagag cgactggtaa tagcggaggc gattatgtga cctatggaca
     8761 atatgagtca gatgacgtcc tttactggat aaatcaagaa gttcgagagc ggccatctca
     8821 aaagatttta ctttatggtg gttcgatggg agcagctacg atgatgtctg tattagcgaa
     8881 agatattcct gtcaatgtca aaggaattat tgaaaattgc ggatttgcat caattgatga
     8941 acaactacgt tttacgtaca gccagacagt agcccccgca ctaccggatg ccataaaaaa
     9001 tcagttagac atcattgggg atcaagagca tgaagattta tttatgggcc ttttgaaaca
     9061 atactatttt gatcaggaga tgcatttgga tacaaaagct gctctaccta cgattggaat
     9121 gtcagattca ttgcctaaat tgattatcca tggaacagca gatgatgtag tacctgtgtc
     9181 taacgcgcaa aaattatatg agttatcagg agggtataaa gatcttttat tagtagaagg
     9241 agcagggcat ggaaaggcac aggaagttga ccatgctgcc tacacgaaac atgtaacgga
     9301 ctttttgaaa gtagtgttcc atgatcagat aaacgtaaag tatgtggatg aaaacaataa
     9361 aagtctactc aaagaccagg acgaaatcca gctttacgga gcttatggag aaaattatat
     9421 gacagagcaa aagacattcg aaggatacga attagcaaat gttgaaggac cgactgaagg
     9481 gatttttaat gagacgatac caactattat tttctattac aaaaaaatcc cagtagctcc
     9541 accaaaaaag caagatccga cagagaatgc agacaataag ggaaagaatg aacctcgaga
     9601 ggatcaatca agtgatgata cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac
     9661 agatgcaaaa aaagaatgtt gccgaaaact ggagaaaaga aacattcgct tctcatttat
     9721 tcaggtggat ttttatctgt tagcacgatt atcacatgga tttggcgaag aaagagaggg
     9781 taaatgaatg aaatggttca agcgaatagt gattgcagta ccagttatca tcgtagttat
     9841 tttagtcact gtatttttga ttaatcaaat cacaccaaag ccgattagct tgcttgttag
     9901 aaaacaattt gacacaagtg aaaaagaaca agtatatgcc cgaccagatg atttccagca
     9961 aaaaatcgaa caaatcgata taaaaaaaga aatatcctat ccttcaaagt acaaaaataa
    10021 taatatggat atctacacac cgaaagtgga aaagaaaaaa ttaccgattc ttttctggat
    10081 gcatggaggc gcatatgtag gaggggataa aaatgattgt cgtgattatt tggaatatct
    10141 atgttcagac acgcagcaaa tcatcgtaaa tatagattat gaacgatcac cggaagcaaa
    10201 acatcctaca ccagttatcc aattgaacga agccattcaa gcagctaaaa aagagataaa
    10261 agatcaagca gattggtcga atatttcgat tggtggaaat tcggcaggtg cacaaattag
    10321 tggagaatat ttgttagcac ttcaaaatga agaaattaaa aaagctgatc atcttgatcc
    10381 gactttggag aacaagcaga ttacgaagtt tatttctttg tctggtctgt tggatccttc
    10441 tgcacttact caagtaagtg ataaaatctc tagctttctt tatgaaaaat gtggatgggc
    10501 ttactttgat aataaggact ttgaacaatc agagaaggtc aaaagtttag ccttggtcag
    10561 acatgcggat cgttggacgc aaaacacatt cttaacggat ggaaatacaa atacgtttac
    10621 gaaacaaatg gaagaagtaa cttctgtatt tgagaaagcg ggggtgaaag tcacaaaagt
    10681 agattatgcg gagaaaaatg ctgttttgaa ccatgaatat cagtttgatt tcagcaataa
    10741 acaagcgcaa gaaacatatc aaaagttagt aatgtttttc aaggagtaaa gtcttatgat
    10801 gcaaaagagg ttgtaatctg tcacttagct ccaagtcaca aggagcaatt tcaataaaca
    10861 agcttttcat ggtttatgaa tattgtaact tgtgactgag gagttagaga tggcaagggg
    10921 caccttttat tcgaaatttg ggaatatcac acgattattg tcacgatctc ttttacgtaa
    10981 attttataag tcagatttgt tgttttgatc gtttaaaaaa agcaacaaat ccgaagcctg
    11041 cggaggcaag aagggacaaa aagccgagtg gtaagcaaaa gaatgctggt tccactaata
    11101 ttccgttgtt attgatataa ggctgggaac aaagatgaat agcaaataaa atcaatgcac
    11161 tagcgaaaaa aagcaaagaa atatggatag ctttcataag cagcacctcc ttttttataa
    11221 ttgtatgata aatagattaa gaatctataa atgtgacaag tttgttagga tgtgaataaa
    11281 atgatcatct agcgaacttg aaacaaagat ggctaatgat aagcaattaa tgattcagca
    11341 atcagatagg tacaaagttt accatcatac gtctagacca ataatagaaa atatgggaat
    11401 ataatagaaa agatttagga tacccccaaa agaatttccc caaaaattcg atgtatccta
    11461 aactttttta ttttacataa caatcatatc aactgtgaaa taaaattaca aatgataacc
    11521 taaaatgagg ccaaaagcca gtgttatcaa ggaaatactt aattcaccaa aatttttttt
    11581 aattttttag aaaataagaa acaaaaataa taaaaaaaag aaaagataaa ttttttacta
    11641 ctgtatacta actttgttat cgcatctaac aagtaactcg aataatacca atacaacctt
    11701 ctccaaagat gcgatgacat acgttctaac ccaccgacta ttgatgttct gtaccaccct
    11761 tgcagaacca aaaaagccac taccaaatat ttggtagtgg ctttttttgt tttatggatt
    11821 tttcgctttt tctgttatgg tgctattctt tgatttcaga aatagtatgt cgaactttag
    11881 cgatagaacg acgtgattct ttttcgttgt aaaacatcaa aatcacatat agagagtcca
    11941 aaatgcttag ttgagaaata cgggaagcta aagcttcaga tcggaattct gtttcttcag
    12001 aaatagaaat aaaaacgaca tcgccaagtt tagcaaggga agaattagct tggctagtaa
    12061 taacgattgt ttttgctcca gtttttctta cggtttcagc aatatggatt gtttcttttg
    12121 attgccctgt gtgagaaatc aaaacagcac agtctttttc cgtcaataaa gaagcttcca
    12181 tcaattgaat atggtaatcc gtgctgtgag aagttgcgat aggggaacgc aaaaatttat
    12241 gataagcgtc agttgctaca atctctgaac cgccaacacc gaaaaaatag acacgtttag
    12301 attcattgat aatagtagcc gcttttttta gatccttttg ttgtaacaac ttttttgtgt
    12361 catttaaagt tgaaatacta gactcgaaca ccttttgcgc catagtgagc tcagtatctg
    12421 ttttctgaat attttcgtgg atagaaatag cagatagatc attttcttgg atcaacatgg
    12481 ccattttaaa atctttaaat ccattataac caagtttttt tgtaaattga aaaaaagtag
    12541 agtcagcaat gcccagttca cttgctaaat tgctaatagg actatgactt actttgcttg
    12601 gattttctaa aatataatcg gcgatagcct gttcttttgg gctgaagtcg ttgtacattg
    12661 ttttgatttg tagtcttatg gagacgctca tgagaattcc tcattttcat aataagatta
    12721 tcattttttc cgttctcatt aatgttaaag atacaaggag agtaggaaaa aatcaaacaa
    12781 aaaatatttt tgtaaaatct atttacaaaa aaattttttt gtataatgag ggtataagaa
    12841 atggaggcgc tatcatggaa attggtgtaa ttggtttggg aaaaatggga ttgaatttag
    12901 ctttaaactt aaaagagcac catcacaaag tacaaggact cgacatttca agtacagctg
    12961 ttagtcaggc aagagaaaat gacattcctg cttatcaaga gatctctgaa tttctagcta
    13021 cttttgagca gaaaaaaatc atctggttaa tgcttcctgc tggagagata accgaaaaca
    13081 tgttaagaaa actttttcca aaactcggac caggagacat tattattgaa ggcggaaact
    13141 cttattataa agattcaatc cgtcgagcag aagagtttca gaaaaatgaa atcggttact
    13201 tggattgtgg gacttctgga ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg
    13261 gaggagataa agaaactttt ctatccgtgg aacaattatt caaagatgta gcaattgaaa
    13321 gaggatatct gtatgctggt gcatccggaa gcggacactt cttgaaaatg atccataatg
    13381 gaatcgaata tggaatgatg caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg
    13441 agtatgcttt tgacttggaa caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg
    13501 gctggctgat ggaaatcatg gaagatcagt taaacgaaca tcccaattta agtaattatc
    13561 ggggaatcgt ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg
    13621 ctgttccagt tccagtaatt gcaactagtt tgttcatgag gaatctttca caggaagaag
    13681 atagtttttc agcaaaagta gtttcagcac taagaaatgg gtttggcgga catgaaatag
    13741 ttgaaaaggg tgagtaaaat gccagattat catatagcaa tcgttaattc cagcagtttc
    13801 ggaagagtct ttccacacca cttggaaaga ttaaagtcag ttggagacgt tcatttcttt
    13861 actgttgatc aagagattcc aggaaaagaa ctagcggagt tacttaaagg attcaatatc
    13921 attattgcaa gtgttacgcc attttttcga cgagattttt ttgaaaacaa agatgaactg
    13981 ctattgattt cccgacatgg tattggttac aacaatattg atttaaaagc agcaaaagaa
    14041 caccagacaa tcgtatctat catacctgct ttagttgaac gaaatgctgt agcagaaaat
    14101 aatataacta atttgttgtc catgcttcga cgcacgacac aagcacaagc aagagtcaag
    14161 gacgatcgat gggaagatcg ggcggagttc gtcggtagga cgctgtttaa caaaacagtt
    14221 ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg taacggtttt
    14281 cgctgtgatg ttctagctta tgatccttac aaatcagatt tatacatcga aagctttggt
    14341 gcaaaaaaag tagaacttca agaattactg gaacaatcag atatcatttg tttatgtgcg
    14401 aatttgacag aagagaacta tcacatgatt tctgataaag aagtcgatca gatgaaagaa
    14461 aatgtttatc tatctaatag tgcaagaggc gctctagttg atgaagaagc gatgattaat
    14521 ggtctgaaaa gcggaaagat cgcaggatat gccacagatg ttttagaaga agaaccggga
    14581 cgtcaagatc atccgtattt agcgtttgaa caagtagtga ttacgccaca tatatcggcc
    14641 tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc
    14701 agtcagcacc ttcttcctga acgttctgtc cagacaaaga gcaactatat aaaaggatga
    14761 tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc tcaattttat ttatatgaag
    14821 aaggtggtat ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa agagtactgg
    14881 tcacccacgg aacggtatca tgggaaaagg cgctacctaa attagtattt ttgaatgacg
    14941 aaacgattca atttttttat catcgttatt ctggagaatg tagttatgct gaagccagac
    15001 gaatagctac aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa
    15061 actgaccgat ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg gagtcatccc
    15121 tactttagct agtaattgtg ctccatggac acctcttgct gtcatgtata aggagaatgg
    15181 acaagcagaa gggaaaacag aacactttta cagacaagct gcttttttta taacagatcc
    15241 ccaattagtg attgattccc ctgttccata tttcattgcc gggttagcag atacactagc
    15301 aaaatggtat gaatctgagc tcatcttaga acaagatttt ttgcaagatg aatcattttt
    15361 gaagctagca caagatactg cgaagatctg caaagaagag attttgaatc attctgctaa
    15421 agcaatccaa gatatggcac aaagaaaact gacatcggag ttcaaacagc tttcagagat
    15481 tatctttgct gttgctggtt tagttggagg attaggagat aaatacgcaa gaaatgcggc
    15541 agcacatgct atgcatgacg ctatcagtaa atatattccg gaaagccatc gttttctaca
    15601 tggagaaaaa gtagcgtatg ggattttcta tcaactggca ctagaaggtc gctgggaaaa
    15661 aatagacgag ttatatcctt tgtatcggga attagaactg ccggtttctc tagaacaaat
    15721 gggattattt ccgaaagata aacagataat agataaaatg gctgaattta ttgattcgaa
    15781 agagaaagtg catttgattc ctatagaggt aacacagaaa tcactgatac agtcaatgat
    15841 ggaactggaa ggttatatca gaaagatctg aaggaggagg acacatggaa acattaagtg
    15901 tagtacaaag tttgttgatt gctttatggg tagccgcggt tatgtcccgc tggttaggtg
    15961 gaggagccac gttgacctta cgtttttctc ctttgatgac tggattagtc gtcgggttga
    16021 tcatggggga agttgcacaa gcgatgattg ttacagccgc tttgcaaatg atttatatgg
    16081 gggttttttc tccaggagga tcaatgccag ctgaaccatc tattgcagca gctatcgcag
    16141 taccagtcgc attattaggg gatctgtcgc cagaagcagc gattgccgtt gcggtgccag
    16201 tcggattgct cggaagttac ttgtatcagt ttcgtttttt catcaatact tttttaggta
    16261 aatatacaga caaagctgta gaagatttga atacaaaagc tatggcccga tcaattattc
    16321 tttatccaac ggttgcctca tttttattat tcgttccgct tgttttcatt gctttatatt
    16381 taggggcgcc attgatcgct gatgtaatca aagctttaga gggaacagtc gttatccaca
    16441 ttcttgaagt agtaggtgga gggttagcgg caatcggtat cgcgacaact gtatacgtta
    16501 tcggtcgaaa agattatctg gtctttttct tccttgctta ttttatgagt gttgttttga
    16561 aatctttaga tattacgatg gttacttatg ccatttttgg gattattatc gctttgatct
    16621 ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac aagcaataac aattcagcat
    16681 cttttgatga cgaagatgac tatgatgacg gattttaatt taggaggaat gggaaatggc
    16741 tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa
    16801 agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac gttacttggc
    16861 gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag atgaagactc
    16921 tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa gctggggtgg
    16981 cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag aagagtatga
    17041 agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg gattgatggg
    17101 ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata tttttatcgc
    17161 aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc cttttatctg
    17221 ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt tcactatggg
    17281 ccgtaatgct acaggcctaa tgcaaaacac tggtgttcag acagcgattg aaatgctatc
    17341 agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc
    17401 attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg atcaaatcgt
    17461 gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg
    17521 attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc tagcaggaat
    17581 cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt
    17641 ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa
    17701 ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag atctttacaa
    17761 aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga aagctaaatt
    17821 ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa aaacagtcca
    17881 aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata ttggagggat
    17941 tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt taaataaatc
    18001 agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata tttattttca
    18061 agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc
    18121 ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga acgagctgtt
    18181 tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa
    18241 acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag atgtgacagt
    18301 cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag atatcgaaaa
    18361 agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag gaagttattt
    18421 catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat tgtccttcat
    18481 tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc ctcatcacgt
    18541 attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga gctacttttt
    18601 cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc gaaatacttc
    18661 atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg tagcgtatgc
    18721 gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat cgggtatgaa
    18781 aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc tacagttttc
    18841 gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa
    18901 aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa
    18961 aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta ttgctctttt
    19021 tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa tcatgatcgt
    19081 tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg tcgggacatc
    19141 gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat tcggagagaa
    19201 ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat ttgttgatct
    19261 aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga ctgaagaaaa
    19321 gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag aagcaatcaa
    19381 tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca aacaaggaaa
    19441 agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg aggacgattt
    19501 ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa aaaggggatt
    19561 cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt ggaactttgc
    19621 tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg aaagcatctg
    19681 ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca
    19741 aggcagggac agatgaacga gattactata ttgataacta tatacgaaca atcaaaaact
    19801 tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt ggatgggcga
    19861 aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac gatcagaaaa
    19921 tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag tcaaagggat
    19981 tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt
    20041 atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta gaaaaagtga
    20101 ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat ccgccatggg
    20161 aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa attctaaatg
    20221 cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg gcaaatccgg
    20281 aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt gtccacttca
    20341 gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc tctacagaag
    20401 gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc gacggtccta
    20461 tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga tatggcctgt
    20521 atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata agtaaggaaa
    20581 aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat ggatggcctt
    20641 ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca aaagtatgat
    20701 tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat ggcgataaga
    20761 gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg agaagcaatc
    20821 aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa aaaaggagca
    20881 gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc ttcgtctaat
    20941 acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa
    21001 atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt tttgaaagct
    21061 tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc gattgcaggg
    21121 atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca tttgataggg
    21181 ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata tgaatccaat
    21241 aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga aagtaaaggg
    21301 ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc ctgcacgact
    21361 gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt atttgtagtg
    21421 aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat gcgtttaaag
    21481 agaagaacga gaatcgtcca tatgagggtt tccactcctt atgcagattt ttttaattct
    21541 tgcactgctt gttcgatcgt aacacctgtc gctacatgat tggtattttc tttgtcaaaa
    21601 acgacaaata gattcaaatc tgtatccaag gttactcgat attgtatatc tttgttatag
    21661 aatgtcatag ccattctcct ttctttaaat acttgctgaa aggcaacgaa cgcgttgata
    21721 acgaaattcc atttataagt ataccaccaa ttgaaaggtt ttcatagtta agaaatttta
    21781 aagtcaaaag ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg
    21841 ttattcaact ttttcttcga ttcgaagaca atagaagaca atgaatttta tatttcattg
    21901 cattatcctt gtgttattag aaaaagcatg atatattaaa tgagtaatct attttgaaac
    21961 gtaatttgag cgatatatct acatagataa aactcctttt gcctatcaaa tatttattgc
    22021 aacgaaaaaa cgtacattga tacgtatcag gaggaattta tttatgaata acggtacagt
    22081 aaaatggttc aacgcagaca aaggttttgg ctttatcact ggtgaagatg gaaatgatgt
    22141 atttgcacat ttttcagcta tccaaggtga cggcttcaaa actttagaag aaggccaagc
    22201 agtgtcttac gatgttgaag atggtcaacg tggccctcaa gcaacaaaca ttgttaaagc
    22261 ttaataaaaa ttgactttta accgtctttt cgaaagaaaa gacggttttt ttgtacgaaa
    22321 aaatt
//
LOCUS       DASHRQ010000043        21923 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.043, whole genome shotgun sequence.
ACCESSION   DASHRQ010000043 DASHRQ010000000
VERSION     DASHRQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.043"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            282..521
                     /locus_tag="U2M88_001796"
     CDS             282..521
                     /locus_tag="U2M88_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374179.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="U2M88_001797"
     CDS             complement(883..1143)
                     /locus_tag="U2M88_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374180.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1328..1825)
                     /locus_tag="U2M88_001798"
     CDS             complement(1328..1825)
                     /locus_tag="U2M88_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEM8374181.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1955..2665)
                     /gene="budA"
                     /locus_tag="U2M88_001799"
     CDS             complement(1955..2665)
                     /gene="budA"
                     /locus_tag="U2M88_001799"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEM8374182.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="U2M88_001800"
     CDS             complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="U2M88_001800"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEM8374183.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4547..5209)
                     /locus_tag="U2M88_001801"
     CDS             complement(4547..5209)
                     /locus_tag="U2M88_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEM8374184.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5219..6034)
                     /locus_tag="U2M88_001802"
     CDS             complement(5219..6034)
                     /locus_tag="U2M88_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM8374185.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6296..6739)
                     /locus_tag="U2M88_001803"
     CDS             complement(6296..6739)
                     /locus_tag="U2M88_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEM8374186.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6873..7211)
                     /locus_tag="U2M88_001804"
     CDS             complement(6873..7211)
                     /locus_tag="U2M88_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEM8374187.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7199..7576)
                     /locus_tag="U2M88_001805"
     CDS             complement(7199..7576)
                     /locus_tag="U2M88_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEM8374188.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7800..8993
                     /locus_tag="U2M88_001806"
     CDS             7800..8993
                     /locus_tag="U2M88_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM8374189.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9159..9581)
                     /locus_tag="U2M88_001807"
     CDS             complement(9159..9581)
                     /locus_tag="U2M88_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8374190.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9976..10170
                     /locus_tag="U2M88_001808"
     CDS             9976..10170
                     /locus_tag="U2M88_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374191.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            10167..10661
                     /locus_tag="U2M88_001809"
     CDS             10167..10661
                     /locus_tag="U2M88_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374192.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(10823..11995)
                     /locus_tag="U2M88_001810"
     CDS             complement(10823..11995)
                     /locus_tag="U2M88_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEM8374193.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12201..13016)
                     /locus_tag="U2M88_001811"
     CDS             complement(12201..13016)
                     /locus_tag="U2M88_001811"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEM8374194.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(13166..13612)
                     /locus_tag="U2M88_001812"
     CDS             complement(13166..13612)
                     /locus_tag="U2M88_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEM8374195.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(13609..14613)
                     /locus_tag="U2M88_001813"
     CDS             complement(13609..14613)
                     /locus_tag="U2M88_001813"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEM8374196.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(14653..14982)
                     /locus_tag="U2M88_001814"
     CDS             complement(14653..14982)
                     /locus_tag="U2M88_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEM8374197.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(15108..17435)
                     /locus_tag="U2M88_001815"
     CDS             complement(15108..17435)
                     /locus_tag="U2M88_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEM8374198.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(18278..18571)
                     /locus_tag="U2M88_001816"
     CDS             complement(18278..18571)
                     /locus_tag="U2M88_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEM8374199.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(18974..20152)
                     /locus_tag="U2M88_001817"
     CDS             complement(18974..20152)
                     /locus_tag="U2M88_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM8374200.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(20256..20570)
                     /locus_tag="U2M88_001818"
     CDS             complement(20256..20570)
                     /locus_tag="U2M88_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8374201.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            20677..20892
                     /locus_tag="U2M88_001819"
     CDS             20677..20892
                     /locus_tag="U2M88_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374202.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(21192..21434)
                     /locus_tag="U2M88_001820"
     CDS             complement(21192..21434)
                     /locus_tag="U2M88_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8374203.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(21421..21849)
                     /locus_tag="U2M88_001821"
     CDS             complement(21421..21849)
                     /locus_tag="U2M88_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM8374204.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         6455 a         4310 c         3706 g         7452 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt cttcaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
     1321 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
     1381 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
     1441 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
     1501 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
     1561 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
     1621 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
     1681 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
     1741 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
     1801 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
     1861 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
     1921 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1981 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     2041 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     2101 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     2161 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     2221 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     2281 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     2341 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     2401 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     2461 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     2521 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     2581 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     2641 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     2701 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     2761 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     2821 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     2881 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     2941 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     3001 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     3061 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     3121 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     3181 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     3241 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     3301 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     3361 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     3421 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     3481 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     3541 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     3601 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     3661 tttcaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     3721 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     3781 aagcagaacc taatttagga tcgctcaatg gcttgatgac attaccttgg acaggtgcat
     3841 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     3901 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3961 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     4021 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     4081 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     4141 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     4201 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     4261 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     4321 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     4381 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     4441 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     4501 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     4561 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
     4621 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     4681 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     4741 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     4801 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     4861 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     4921 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     4981 tttcttttgc taattcttct gtcataccta tcatcacgat gcaacctgga ccaataaaaa
     5041 gtaaagaaaa aataaaggaa aagacattcc ttccatctat cgacaaaagg aacgcagctg
     5101 ctatatacaa gatattgaca caccaaaaac aaaggctact tgccaatgat tgatacaacc
     5161 aataaaagaa ttccatataa acatttttgt caaaacgctg ctctttcata atcacctcct
     5221 attccttcat tcctgatgaa gcgatccctt ctacaaaata tttttgcatc gctaaaaata
     5281 taaaagcaac aggaagaaca gaaacaactg aggcagccat gattaatgcg tactctgcat
     5341 caaatagccc aataaacatt ttcaacccta actggattgt tttattttcc gtagaagaca
     5401 aataaatgaa tggccccata taatcgttcc atgtattaac gaatgtgatg atcgtcaaac
     5461 tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg atagattccc cactctgata
     5521 agccatcgat ccgcgcactc tcacataaag aatctgggat actactgtag tattgcttca
     5581 acaagaacac accaaaagca ttgaacgctt gtaatagcac caacgaccac aacgtatatg
     5641 ttaaacccac ttttctcatc ataatgaact gcgggatcat ataagactgc caagggacag
     5701 caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc tttaaaattt agtttagaaa
     5761 acccgtaagc agcaaaacta gaagtaaaca actgaatcaa cgtgatcaca acgcttaaaa
     5821 aagctgtatt cttaaaaaat gtcactaaag gaatacgaga ccaaataacc agataattct
     5881 ctaaatgcca agttttaggg atccattgaa tcggaatcgt gaacacttca ttatttgctt
     5941 tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt caataaagct aatacaatca
     6001 gcagaatcat acccaaccat tgaataaagg gcatgttttt tgaacgaatt tttgcttttt
     6061 ttctttcatt tcgaacacct ccaaagttat aatcttcatt tctttgtaat gattacaatc
     6121 cagaacaacg gttattatag ctgattcatt tccgttcttc ttatgtttgt aagcgcttat
     6181 ttaacaagtt atgtaaatct ctttattttt tttgatttaa aaatgaaaaa atacaaaagc
     6241 aagactgtca tacgtctcgc ttttgtattg aaatttcttc gattagagat acttattact
     6301 gttcaaaagc ttctttgaaa cggtgaatca attcattgac tacaggtgat aaaatccgat
     6361 tctttttcca gactagcatg cagttcgttt caatcattgg gtcaagcgga acgaatgtca
     6421 ctcgctttga aagatgactt acagcacctt ctatgactaa agcagcacct acctgattct
     6481 caattagcgc aaatacattg aaaatcaaat tgtacgttcc tgtgatcagc aactgctcct
     6541 tttttatgcc taaccagtca gcaaacattc gctgtatttc tggacgcttt gagatcaaaa
     6601 gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt ttttgctaaa acagaatttt
     6661 ctaaaatgag caatccccat ttttcctttc ttggtaacaa aatcgtttcg tatttctctg
     6721 tatctactgg atctagcaaa atcgctaaat ctaacaggct tttttctagt cactccgtga
     6781 tatcatcact tgttccgctg tggatatgaa attgaacgct gggataatca cggatcaact
     6841 tttctagtaa tagtgccaat gtattcgaat tatcagcttc cacacagccg atcgtaaatc
     6901 ggccgctgaa taattgtttt tttctgttct cgaattcttg agtggtttga ttcgttaaat
     6961 ctaaaatttc ttctgctcgg cttttttaga aaataacctg cttctgtcaa gatcattttg
     7021 cggttttcgc gtacaaacag ttctgttcct agttcctctt ctaactcctt cagttgtcgg
     7081 cttaaggttg gttgtgtaag atgtagaact tctgcggctt gagagattgt tccttcttct
     7141 gcaattgtcc aaaaatattt tagtaagcgt aactccatag cgatgctcct tgattttttt
     7201 acttgataca ttccatttta tttctagata acacacgatc atatttcttg atttttcctt
     7261 gtaaacgttc caaacactct tgcatttcag caatttcttt cgtcaaaagt tcactttctt
     7321 caatcaaaag cttacgccgc tcaaatacag ttgcttctcc ttcgctagat aggcgaatat
     7381 attcaatcaa agactcgatc gataatccgg catttcgcat acagatcgta aagtcgaccc
     7441 attttaggtc ttcttctgta taatcacgga ttccgccttt tgtccgatta actggtggaa
     7501 tcaacccgat tcgttcataa tagcgcaatg tatcggccga aatgcccttt tcttcactga
     7561 cttgtttgat attcatctga tcactctttc tcttaatttc ttgccacttt acttatgtat
     7621 tttatttatt ctacaacttt aagtgtactc caagtcaata ctcatttcta ttaaaactaa
     7681 gctggattat ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc ttgttttttt
     7741 attctttttt ggatatactt ttaacaattt agggaaattt taggaaaagg ggtataaaaa
     7801 tgacaacgta tcagcgaaag gtattattat cgacatcggc gggaattgct ttagagaata
     7861 tggatattat gtttttagct ttttcattat cttcaatgat tacttctttt catattagtg
     7921 gtacgcaagc gggtttcatt tcaacgatca ctaatttggg gatgctcatt ggaggtattt
     7981 tttttggcat catagctgat aaatatggac gtgtgaaagt attttctcaa acggtccttc
     8041 tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa catctacttg gtttatctct
     8101 ttcgctttat cgcaggtgta ggagcgggag gagaatacgg ggcgtgtatg tccttgattt
     8161 ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc ggttgctggt attggtgcgc
     8221 aggtcggtgc ggctttggct gcgattttag ctgctgtgat cattccaacg tttggttgga
     8281 agatgttgta tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt cgtcgcggct
     8341 tgactgaacc aaaggaattc aaatctgtac aatctgccga aaagaaaacc aaacttagca
     8401 gactgtttga atcaaaacat cttgcttggc aaacatgtgg ccttagtatc atggtaactg
     8461 tccaaattgc cggctatttt gggttaatga attggctgcc ttcaattatg caaaatcgat
     8521 tagggctttc ggtttctggg tcttcattgt ggatggtcag tactatttta ggcatgtctt
     8581 tggggatgat gacttttggc ataatcatgg ataaattggg gcctaaacta gccttttctt
     8641 tgtttttaat ttgttcatcc ttatctgtct atctacttgt gttagcccat agccagtgga
     8701 gtttggtagc agccgctgtg atagtcggct actttatcaa tggcatgtat ggtggatatg
     8761 gcgcagtgat tagtagtttg tatccaacag aaatccgcgc gacggcgaat aatttcatta
     8821 tgaaccttgg acgagctgtt ggtggttttt cttctatcgt catcggtttc ttgatggatc
     8881 attatagttt aactgccgta atcttgtttt tgagcggtat ttacctagtc agtctgctgg
     8941 tcttgacaac tcttactggt gttacagcat taagggaaag cttgcagcat tgatagttga
     9001 gtctgtaaat gacaagaaag aggtgtggca aaagcttgca tctgcggtaa taagaccagc
     9061 caaatcaaaa acatgagaca ctgtttccgt ttggctggtc gtattacttg atgcagggca
     9121 gctttttcaa ctgctaattt ccttctatct ggcggcgact attctttgac gtccgtcatt
     9181 tcaaagtact ttactaataa cttagcataa cgaaaaactg tggatacact cgtatattga
     9241 gcgtctgcat aatcttccaa atttttctca ggatgttgat acatgaattc aatcaaatga
     9301 taattatatg attttctgta gaaatgaaga taaataatgt agattgggaa gttctcctgc
     9361 ttttccatag aaaagcgccg attattctca ttcttcaaaa tgaccttatc aggatatttt
     9421 tggaacaatt cttctaaatc tgttagataa ttcaagatag acttacttga taccttcaat
     9481 tcgttttgta gttcttctat tgaacaccaa cgttctgttt caagcagtaa atgaatcatt
     9541 ttaattttct tttgagaaac ttttgttaaa aaagtctgca tattttctcc tcctgtatct
     9601 actttactat actacataca agaaaaaaaa cggatacaaa tatcatattg tcacaccgtc
     9661 aatagtgcat aaacctgtta ttatttctct ttttttccta agtattctat tgatatgaat
     9721 catttaatcg tttattatat aaatgtcaat aatctaataa taaaataacg tacatatttt
     9781 attattgcat acttaataac agaactatag caaaaaatcc cgtgtatcaa cacgggatta
     9841 gattaagttt cctcaatcac tattcataac acaattatta taacaagtaa gtaaacaatt
     9901 gaaaaggaca tttattgaaa acggttgttt tttacttaag gaggaaattt agcgaaaaaa
     9961 ttagttgcat tgtttttggt cgcaggaagt ttgttgagtg ggtgtggtgc tactacgtct
    10021 actactcaaa gcacaagttc agatcaggct tccagcagca gtaccacgat ctccgaaagt
    10081 acggcagatt catcgaaaga acaagaacaa attgaaaaga aaaaagaaga gaaacaaaaa
    10141 gcggaaaaag ctgcactaga aaaaaagtga cagaagcgga atctgctttg tcacaagctg
    10201 aagcaaatcc tacaaaagaa aactatgcaa ctgcttcagc agctatccaa gcgctacctg
    10261 aaaacaaaca agagttgaca agccgtcttg ctgcaattga ttccacaatc aaagcaaaag
    10321 aagcagaaga agaacggaaa aagcaagaag aaattgcacg acaacaagca gaacaagcgc
    10381 aacaagaagc aatcgcacag caacaagcag aacaggcgag gcaagaagca atcgcacagc
    10441 aacaagcaga acaggcgcga cagcaatcag cagctgaaca ggcctctcag caggcacaag
    10501 ccgcagctcc tgagcaaaat aatgaacaaa ctgtctatgt cacaccgacc ggttcaaaat
    10561 accatacaca taaatgtgga aatggtacgt atacccctgc cactttaagc gaagctcaag
    10621 ctcgtggttt aaccccttgc tccaaatgtt atggaaacta attcaaaagt tatactataa
    10681 taataaaatt cttctgaaac ataaaaaaat agaagatggc gttccagata gtaaaccaaa
    10741 aataacagtt tacacgtcca tttcgtcagc ttctctcttt tttcatttag tctgttccct
    10801 ctgctttttt ggacatcttc attcattctt tctcaatcaa atagtacacc tctttttctt
    10861 ttcgcagttt atatcttcct tttttcaatt tcattgaaaa ctgtttcgaa tgtttatgtt
    10921 ttacatggat tttaagcagg agctcctcat gttgcaagcg atagttcctg ccttttaaat
    10981 cagaaaatct cactagtccg tattcatcag aagctttcac aaatggaact ttttctctag
    11041 taatatagtg tttgccgttg agcgttttta cagaaaagac cattccttcg attggtcgtc
    11101 cgtttgtgtt ctttacataa aaagataaag gatagtttcg cttagcgatg gccaaagaat
    11161 ctaaataagc gatgagtagc caaactaagc cgattgatcc aagtagtata ccgataaacc
    11221 aaataaaaaa tttccatttt ttccattgat caattccttg gatcgctttt tttgcctctt
    11281 ttgcttgata aggaatacga tgccccgtca caagcagtcg atgtgtattg accatataag
    11341 gagtacaggt aagcaaagtc acgagatctc tgcctttttg gatacccaaa gaatctactt
    11401 catcaggtaa gaccacttgg attttttcta cttgatacgc caatgtcttt ccattgatct
    11461 ggatgaaaaa ctggtctcct tttttcaact taggcaaatc tgtaaataat tttgcttctg
    11521 ggagtccacg atgcccagaa agaacggcat gggtgttttt cccgccagtc ggataagaac
    11581 tttcttccaa taatgacgtt cctttttgca aaaatatttc cgttgtctga tcgaatatag
    11641 gtaagctgac attgattttt gggatttcga tgactccaag catatgttcc ataaaaaagg
    11701 cattactagg taaatctttt aatgcttttg catcaactgc ttggttgaaa ctgcctatcc
    11761 ctggtgatac gttttgctct gccaactgtt ggtttttctt tgtcattttt tcctgctgtt
    11821 tttttatttc ctctgaattc ttattcgatg cttgcttttg ataatggatg atcatttgct
    11881 gggcaagaaa atcattcagt gaatcttcta caaaaggata agaaaagatc ccgatcccaa
    11941 cgactaaaat gattgccatg aagatgtcca gtattcttat tctgctttta ttcatcatgt
    12001 ccacctattt tctaatatgt atatgaagag gttgtgaaat cagcgccttg acctgagtat
    12061 tagcgaagca aacggcaaaa tagttcctcc tattttgccg tttgttgagc gaataccaag
    12121 agaccgacca cttgtcacaa cctttctccc catttgaatc actatcgttt atttatcgta
    12181 tgatttttgt ttccaatagt ttaagcttct gtttcttgat ggcgtttttt gaaatagatc
    12241 actgctcctc caactaaagc agtacctacc aagatgaata cgatgatccc cataccacca
    12301 gttgaaggca agattccttt aggttggtta tcgatcgttg tttcatcaga agttgttgga
    12361 tcatcatcta ctgtaaattc agtgtacgca gcttcattct taggtaatgc gtagccttct
    12421 ggtgcttttg tttcttgaag gcagtatgcc ccttgtttca gcccttcgat tgaaaatttc
    12481 ccttctgaat cagaaatcaa tttcacttct tcccagtcag aacctgtgac ttcatctgac
    12541 caagcatatg ggtattctcc tgaggcaagg tttgctattt ttttcattaa tttaacagtt
    12601 ggatcttttg ctcttagaat agaaaattct gcatcggcta atttttcttg cgtgttcaca
    12661 tcttgtttga tgaagtggaa atcaccgtag ttttggacat tttctacagt aatcgtgtta
    12721 tccgtagttg tattttcccg attgactaca ccgtttggat tcccaagatt ggcttcatct
    12781 gcgccatgga tgataccggt tgtttgttgc aattttccgt tttttactat aaaagtagtt
    12841 tctttttgtg gattggttaa ttgataacca tctggtgctt ttgtttcaac taaagtataa
    12901 ttgccgtcta ttaatccatt gacactgatg gatccttcag tatcagttgt gaatgtcaac
    12961 gctgactcag caggatcata gagagcgaca ccagtgtttt catcaaaacc ggtcacgaat
    13021 tgattctctg aatttttgat ctggaattgc gcccctgcta acttatctac ccctgaaaag
    13081 ttcccagctt tctggatttt gatattgctt tctttgatga catttttagc atataggtga
    13141 atcgtgtcta aaggtttgtc agggtctagg gtaagcttaa cacgatattt ggcgacgttt
    13201 cgacaccggg ctttggtgtt tctacaaaga cgtatacagc atctttccca tcagatttac
    13261 ctggaagagt caggttagct aatccttcac cagaagtcgt tacacttgcg aagggtgtag
    13321 cgtctgctgg aactgctgtg tctttacttg gatagtacgt agcagtgatg tcatatacgt
    13381 tgaattcaac gccgttcaac ggttcgctat tcggaaatgt caattccgtt ccgttgttta
    13441 tttgattttc tggtgctgaa gtgaatttca atttatgcaa attaaccgta acattttctg
    13501 gtgtaggtga tacggctgca gaaacctctg cagttcctag gccgagaacg aaaggaagca
    13561 gcatgatagc agttcctaaa aacgtccata tttttgtttt gatgtttttc attttatttc
    13621 cctcctattg tttccttttt gttgtttttt ataaaaccaa gctgtccctg ttgaaaggac
    13681 tagcaataac cctaaaattg agaaacctcc aagcatctct tcgcttgttt ttggaaagct
    13741 tcctccattt ttttcatttg atggaccatt tgtctgcatc tgaactggcg tttttccatt
    13801 attcgtcgga tttgcttttg gttcgttgat gatttccaat ggtataggat gaagcggatc
    13861 ataactgcct ttttcaactt caaagagaat ggttgattga ctcaatatat atcctttagg
    13921 cggtttaatc tccactagtt cgtaacttcc aaaagacaat ccatggacag aaaatgttcc
    13981 atctttactg gaagtaaaaa tcgttgctct tgaatcatct ttggctaccc aatgatttat
    14041 cccgttttct tgcgctaagt attggtcatt ttttttgact agaaattgag catctgctag
    14101 tttttgtttc gtttgccgat ccactttgac aaattgtttg cctcctgttt ggatggcaag
    14161 atgtgtttct actgtttgat tgtcagtcgt caaacgtaca tcgttatcaa atgtcgtttt
    14221 ttcactgttt tctttcaatg tcatttggta acggatcgtt aatttttttc cagcaaacga
    14281 actaagatct tttccagaat ctgataagac aaaaccatgt tttatttttt gtgtgtgaaa
    14341 tgtatgaatc ttgtctccac caatcaaaaa atcgatcgtt tctggttcta gccataactg
    14401 aggatcagct gtatctttga tcgtataggt tcctatctcg ttgatgttct caggaattgt
    14461 tacggtgatg agataaggga ctttatcccc atctgcaaaa tctgtctgct tattctctac
    14521 tgtttttgtt aatgtgagtg cagcaggtat tttttgttcg ctctttggat acaagtggat
    14581 ggtcgtcaat ttatgattag atgaatctgt cacaggaagc acaacgacaa atggtgctga
    14641 tttttctttt acttaatctg aagtctttat ttccgtaaat cggtaaacag cgtcacgacc
    14701 ttgctcatct ttgtccgatg cactaaaaga agcaatgccc tcttcctcgt ctactgtttt
    14761 tgtcacattt tctgccagta ttttttcact atcacttttt tgtgctagtt ttctttgtgc
    14821 gtcttccaca ctacttcctt ctgaccgcag cttgtaaaat tcatccgaga catcatatat
    14881 agcgaaagta acgccgttca acccttttac tccttgaaag ttatccggga tatttattcc
    14941 gtcgttttga ctgatacttg gcatcttatc gctttcgaac attcgattat gcaaagtaaa
    15001 tgtaataggt gattctctag ctgacacagt cggagaacct ataaaaaaga ccagtaggat
    15061 aaaaaagaga acagcaaata tatccacacg tacatttttt gtttttttca tcgctgttca
    15121 cctgcttttt tcttacgatg gataaaataa aagaagccag aaaaaataaa gatcagtcct
    15181 aaaaagacaa agggcagtag acctatccca cctgtcgaag gaagcaagag agtcggattg
    15241 atcgtcaact tagcggtatc tgttgcgata atcgtataat tattgtttac acttccttcg
    15301 cctaaactaa tcgttactat ttgtgatcca atatctgtcc ggctgttaac tgttagggta
    15361 taatccaatg attcgcctaa gacaacaccg gtaacgtgga gatttccaac cgaaatcgag
    15421 tgactttggg catcataatt aaaggcagcg tcatcgacag taatcactgc gttagcttta
    15481 tagatggtat aagttcctga tgtgttggct atatcagtaa atgacaactt gggatttgct
    15541 gctttgactt tgtctattcc gctattcgtt agtttaattg tatagctgcc agcattaacc
    15601 atattttttg ctgattgtgc atctgctgtg taatccatac tgctaagtgc cacttgtact
    15661 ggagtaagcg aactagtgac accctctgct agcttgacat ggattgacgg gacacctgct
    15721 tttccatcat atgtcttact gccactgagc tctaccgcac cgctgatttg cttaatcgta
    15781 tagacatcag atgtctgcac gatagtaaat aaatagtttg ttctggcagc tagtttagct
    15841 agtccgcttt ttgtcaattg ccaagacgta taggttccaa tttcagtagg cgcagttact
    15901 tcaactccct tttcattcat cgctttgaaa tcagttggat tccatgtgat ttgatcaata
    15961 ctgagatcat tgtttaaaag ggatgttgat tctgttttat cattaaaggc atatttaacg
    16021 atcggtacaa cagaaagacc atcatagtac ttactgccat ttgcattgat tgatactgtt
    16081 gcatttgtct tataatatag gatgacttca gaaatctgtt ttccatttgc gtcctttacg
    16141 attacatttc catttgtatc taaactagct aaaacactca ttgctgattg gttctttgtt
    16201 tctatcgctg aaacagctgt aaaggtatat ccgttgatag taggcgtatt agtcgtcagg
    16261 tcttgtgcag cacttccgta agtaagattc attattttag aaggagcaat catctgtcca
    16321 aaatagtctt gatacttaat agtgattggt gtttgtttgc ctgtataggt caccgtatcg
    16381 acgttacttt ctagggctgt gaaagaaccg tttgtttgtg caaaagaata gcccgttact
    16441 gctgttggag tgataaccac tttgtcaccc acatggattc cagataatgt agtcccgtac
    16501 gttccgattt ttgctgctcc accattgact gtataagaaa aagttcctgt ccgattcgca
    16561 ttgacctgaa ccgttgctgc ttgataggtt ggaaaatttg aaacatttcc cttaccatca
    16621 acgatctgcc catttttgat cgagtacccc gtgaatggaa tatcatttgc taactgtata
    16681 ccgattgaaa aagtactcat acctcctccg gcaggaagcc ctccccatgt tgagggtgta
    16741 aaagaataac ttttatccgc attgggtgta actgaatcga tcgtatttcc agcggtagcc
    16801 acccccatat agatactttt tgggaatgtg atagtagtac catttactgt ggagccaata
    16861 aacagattat ggatatctgg acggttatca ataaaagcga ttgctttggt tacgccttgt
    16921 aacgcagttt tatccgcact catcgatccg ttaaccgcaa caatgactcc atctttggca
    16981 tatatcactg tatatccatc tggtatggta tgtccgttga aattcgcagt ggccgcaaca
    17041 gtactaaatg tctggcttct acctgtttct gaataagctt tctcccctgc tgcttttgct
    17101 gctgtgattt ctttttctgt cggatcaatc agttgagtcg aggatgtggt tgaggttgtc
    17161 tcgttgttgc tagactgatt ggtgctttct atggactgta tttcagaata gggtggacta
    17221 tttgtctcct ctgtcgtttg gttagcaaaa tttgacgttt ctgtataaac gtttacataa
    17281 atacttgata agctccctaa gaaagtaaaa atcaagatga tcgaaatcat aatgggatgt
    17341 aagtattttc ttttagatac agctttgagt ctagaaacaa aatgtttttg acatatacgt
    17401 tggattttat tttgagtttt cttaatcatt ttcatccctc ctctcaataa aaaatcatca
    17461 aataatcctt tacacgttat tctaaattta atttttatta ttttattgat gtatagaaag
    17521 tttggggctt tttttctctt tttattctaa ggttatttta gtcttgtcct ttaatacaaa
    17581 gattttattt ttaaataaaa aatactcttt ttttacgatt attgaaaaaa aaagataaaa
    17641 cttgattaat tgaagtattg cttaaatttg gaagctatga gggaagaata aaagagttag
    17701 gacgggatta tctactattt ttctttgttt taaatatttg ttatctttga acataaaaga
    17761 gaaatgtgtc gactgcagtg ttgtgtccct catcacgaat aaacggtgtt caactagctg
    17821 atcttgtatt cacttaaaaa atggtaaagt acgagttgct attccccatt ttcaattttc
    17881 taacgctcag gacaaaatac tgttttcaca acctcattgg aataaaagca tatatattgt
    17941 ttcaagaacg aaatcttgtt atctttttaa agggcatctg tccaaaatct aattttagga
    18001 ggtacgtttt ggtatttatt cgtacaaata aaagagaatt atctttattc atgtaacgca
    18061 tccaattgat tttttcatgt acgctcctgg aacgatttta ctgacgattg cttatttaac
    18121 tgcgaatcgc tcattagctt ttatcatagc tatccatatt ttgaataacg ttcttggctt
    18181 tgtattatag atacagatga caaaaaaagt tcaagtatgc ggtgtagaac caatatcgca
    18241 tacttgaact tttttcaatg agtataaatt aaaacttcta attttcaatt cccatcatct
    18301 gatatgcccg accgtctgaa cttcttttga aaaagccaaa tgtgattaga taacgtcttt
    18361 gttcgacata atcgccacta gaaaatacgt tcagcagtat cgaattaacc tctgtttctg
    18421 tataagtcac tccatggtca aagcgtttca agacttcttt caataggatg tatttctttt
    18481 ttgttgaact tggtacttgt tttacttttc catcattcat gaatgtgtct aaaactttca
    18541 tcttttcttg ctccgtataa gtataaacca tcttctttct ccttttgatt ctaagaatac
    18601 tccatttatg ttagaatatt ctggattttt tattaaagcg gttactctta agaaagatat
    18661 tatcatagtg ctcttttttt tactactact tataggtaga tttattctaa tccattattc
    18721 tcccctacca ataattaaag aaaaaactgg atcagggtgt tcaaattaca tgctaagaaa
    18781 aaagaaccct tgaataccaa gggttctttc tcagtctgat aacaatgatt aacgacggat
    18841 ttctttgata cttgtgaaaa gtcgttaaag tattgatata aaaggatttt tattacctct
    18901 gactacatat tgactaattt gttaaaaaat cgtttttcaa gaatcctgta ataccagtgc
    18961 tttcaccgtt tttttaactc tctaaagaac taaaagcaat cagaggtttt accttttcat
    19021 ttgacgttaa gtggcgataa attttcgaag ttgtttggtc agtatggcct aagcgatctt
    19081 tcaagacttc ccagctacag tgttccgcta ctaagcttgc cattgtatga cgagctaaat
    19141 gagtatgaaa tggcttatta attcctgagc gattcacgca ccgtttgacg tgatcattta
    19201 catactctcg acgataagga acaccacgtt tactagaatc aagatacaca aacaaatatg
    19261 tttcttctat aataaacctg taatgttgcc tcatctcacc caaagcctta atcaactgtt
    19321 gtagcttttc cataactaga ggagttacta gaactcgtct agcagaacta actgtcttag
    19381 tagtttcaat tcgaaaatcc ttactcttac ctttaactgc cgtttgctta tagaacagaa
    19441 tttcatttct tttgaaatct atatctccag gctgtagagc aagcgcctcg ctgactcggc
    19501 agccagtgta aaatagaaca tggaaaagta cagacatcgg taattcctca tgaacaatgc
    19561 cataattcaa aaaagttctc aattcatcga cagtaagaaa cttgtcctca atattattta
    19621 tggcttcttt taaatcagtt accgttaact gatattttgg cattctcaca gacttcattg
    19681 gattttcact tatcaaattc tttctttgac aatatttaaa aatcatattt agaacttgaa
    19741 taagtgattg taaactattt ttggcatagc caacatttgt tccattaacg ccacattcct
    19801 ttaaggtaaa aagcaattct tgaaggcact ctggtgtcaa atcagacaac tttttatcac
    19861 caatccatcg aacaataatt tttaattgtt cctctcgctt atcataggtt atttcctttt
    19921 gttcatttaa cgaacggtaa tattggaacc attgctcacc tacaacacta aacttgtcat
    19981 tgcttgcaac aatttttttc ttaagccttt gttgtttctc catttccaga taagcgagtt
    20041 gcgcctcacg ttttgaatta aagccacgtc ttgttgttcg atatgcttga aagtaccatg
    20101 tgatcctacc agtagaactg atttgcttct tgaatcgatc tgttttttcc atttattgat
    20161 tcctcctatt ttgaaggaac ccaacaagca gaaacccttt ggaaatcttt ctatctatgt
    20221 catcattgta ccgcatctta gaaagaaaat gtaacttata aagatcgaag tagtcgcaat
    20281 ttttctcgat agtcattcga atcactcttt ctcatccatt ctaaaacatc atcacgaaaa
    20341 tagtgtcctc gaatcccgta ccaacaggga ggaaactcac caagttttac taaacggttc
    20401 agtgtactat cacttatctg aaatagtcgc tttaaatcag tatttgttaa taccagaggt
    20461 aaatgactga ctgcaagcaa tttttccgtg taatcttcca gttttttttg taaaattgaa
    20521 tagaactcta tttccagaga attaacattt gttttaactt ccaactccat cacccttctc
    20581 tctcaaaatc aaaacgacca ccacggtacc ccttaaaatt gtaaaaaaac actccagtac
    20641 tccatcaaaa cggactagaa aagaggaatt tttaatatga aacaaggaat tgcagacata
    20701 aaaataatta aagaaattct tgaaaaatct acggctaatg caatagcatt cggaacagga
    20761 atcaacttga gtacagtaaa aaaacttaaa agtggagaga gagctgaaga aaaattaaat
    20821 cttgccgatg ctattaaaat cacagaattt ggcatgaaaa atatgccaac caaaatcgaa
    20881 atatggaaat agtagttttt ctgatctatc agctgttttg ccaaagcaca taggaatctc
    20941 acctcccccc agtaatggcg ggccgatttg gttacggaag tatcattatc gtagtcgcga
    21001 ttctcatatt aaaatttgtc gcttaaaagc taaccttgtc atggcgtaac tagagaatca
    21061 aaatctacat gtactgatag atttgctatt catttgtcaa agagcgctag gctattttca
    21121 gcctatggaa atcctttatt ttgacccgat aaaaaacttc catagaatga aaaggaaagg
    21181 gaaaaaatac tttaacgagt tgtatcaaat tcaagtatct tctcaatcaa ttttgtttcc
    21241 agttgtctac gcatatattc atctacatac ataaccattc ctttttcttc ttcataaact
    21301 ggtttcatgg ctaaccgtga aatataattt tgatattttg acagaacgat ggacatggca
    21361 attgaatcac cagaagctgc ggctttgatt gtcgttagtg gtatgaatgg tatatatcgt
    21421 ttattttgct tcatatttga cacccaaata ctttcctagc tcttccaaag ccatttgtcg
    21481 ttggtaccaa acactgccga gacttttccc aataatatcg ccaatttcac gatcattaag
    21541 accgatgaag aaggacataa aaatgatttc ttgtctttga ctaggtaatg ctgcaatcgc
    21601 atttgccaac tcctcattgg aaataaagat tttcatacca aggacaataa aagtaccact
    21661 actattcaaa tttggttcca aataatagaa ctctgtacct aacccttttt gttccaatat
    21721 ttccattgat atttgccgtt ggcgaaatct agcgtacttc ttttgaatat ttaacgcctc
    21781 attgcagact actttcttac aaaaactgtc aaacgtatgt tgaatgtggg tttcctctga
    21841 gtcgcccata atattctccc cctcaaaaaa ttcattcaac tcgttttact tctatccctt
    21901 tccattatta ctacaatcta gga
//
LOCUS       DASHRQ010000044        21838 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.044, whole genome shotgun sequence.
ACCESSION   DASHRQ010000044 DASHRQ010000000
VERSION     DASHRQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.044"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(269..802)
                     /locus_tag="U2M88_001822"
     CDS             complement(269..802)
                     /locus_tag="U2M88_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEM8374205.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1666..2418)
                     /gene="xth"
                     /locus_tag="U2M88_001823"
     CDS             complement(1666..2418)
                     /gene="xth"
                     /locus_tag="U2M88_001823"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEM8374206.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            2531..3451
                     /locus_tag="U2M88_001824"
     CDS             2531..3451
                     /locus_tag="U2M88_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEM8374207.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3524..4459
                     /gene="murB"
                     /locus_tag="U2M88_001825"
     CDS             3524..4459
                     /gene="murB"
                     /locus_tag="U2M88_001825"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEM8374208.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            4757..6235
                     /locus_tag="U2M88_001826"
     CDS             4757..6235
                     /locus_tag="U2M88_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8374209.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            6512..8572
                     /locus_tag="U2M88_001827"
     CDS             6512..8572
                     /locus_tag="U2M88_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEM8374210.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            8655..9224
                     /locus_tag="U2M88_001828"
     CDS             8655..9224
                     /locus_tag="U2M88_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8374211.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(9399..10205)
                     /locus_tag="U2M88_001829"
     CDS             complement(9399..10205)
                     /locus_tag="U2M88_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374212.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            10343..11857
                     /locus_tag="U2M88_001830"
     CDS             10343..11857
                     /locus_tag="U2M88_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEM8374213.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(12260..13243)
                     /locus_tag="U2M88_001831"
     CDS             complement(12260..13243)
                     /locus_tag="U2M88_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEM8374214.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(13248..13895)
                     /locus_tag="U2M88_001832"
     CDS             complement(13248..13895)
                     /locus_tag="U2M88_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM8374215.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(13980..14612)
                     /locus_tag="U2M88_001833"
     CDS             complement(13980..14612)
                     /locus_tag="U2M88_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEM8374216.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(14628..15179)
                     /gene="coaC"
                     /locus_tag="U2M88_001834"
     CDS             complement(14628..15179)
                     /gene="coaC"
                     /locus_tag="U2M88_001834"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEM8374217.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(15176..15913)
                     /locus_tag="U2M88_001835"
     CDS             complement(15176..15913)
                     /locus_tag="U2M88_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEM8374218.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(15910..16899)
                     /locus_tag="U2M88_001836"
     CDS             complement(15910..16899)
                     /locus_tag="U2M88_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEM8374219.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            17157..17699
                     /locus_tag="U2M88_001837"
     CDS             17157..17699
                     /locus_tag="U2M88_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8374220.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            17716..18801
                     /locus_tag="U2M88_001838"
     CDS             17716..18801
                     /locus_tag="U2M88_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8374221.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            18813..19619
                     /locus_tag="U2M88_001839"
     CDS             18813..19619
                     /locus_tag="U2M88_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8374222.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            19620..20447
                     /locus_tag="U2M88_001840"
     CDS             19620..20447
                     /locus_tag="U2M88_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8374223.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            20444..21517
                     /locus_tag="U2M88_001841"
     CDS             20444..21517
                     /locus_tag="U2M88_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM8374224.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         7158 a         3848 c         4351 g         6481 t
ORIGIN      
        1 ggtaaacggt gtccaaaagc ttgcatctgc ggtaataagt ccaaccaaat gaaaaatatg
       61 agaagctatt tttgtttggc tgttcttatt acttgatgca gaacagcttt ttcacaacct
      121 ctccaataaa cggtgttctc ctgccagctc ctcggtcacc agtcacaata tttaataaac
      181 atgagaagct attttttgaa attgctcctt gtgactcgga gtgaaacggc aggctcacga
      241 cctctttgtg tgtttcataa gtattctttt actttatagt cacaagtttt ttcaatggat
      301 ctgtcccgaa ttttttttct tgataaagca ggatttccgc acatgcacga ctatcttcca
      361 atgcatcatg gtgatgatct aattggatgt tcaaattttc gcacacagtg ttcaatcgat
      421 gatttggaaa ttcaggaaac aggcgacggc tacttttcac tgtacataaa gaaagataat
      481 taggctgttc aagatgataa taatctaagc atcccgctaa gacctttgta tcaaaagcag
      541 cattatgtgc aacgactaaa cgattttttt gaaaatatgg ttggatagtt tgccagactt
      601 ctgggaattt cggcgcattg cgtacgtctt cttgatggat tccgtgaatt tggacatttc
      661 gccagaaaaa aggagtctca ggctgtatca atgtatagaa ttcatcaacg atctggctat
      721 tttttaccat gactagcgca agagaacaag cactataagg ctgatgattg gcagtttcaa
      781 aatccatggc aacaaaattc atttctgtaa aacctccttg ggaaattact gtaacatact
      841 tttgaaaaaa gcaaataaat ttaacaaaaa acatagaatt ttttatgtaa tatttgttga
      901 gaaaagctag tttttgaact cattccttgt gatttggagt tagatgggga tcatgacttc
      961 tcggtattaa gtcaaattta tgcaaagttg tgaggaacca ttttcaaaaa aatttcttac
     1021 gactcgaaag taggcgactt tttcacaact tctccactaa acggtgttca atcagctgac
     1081 cctcggtatt agctcaaaaa atagcaaaat atgaggaact attttgccat ttgtttctct
     1141 aatactcagg tcaggacgct gatttcacaa cttctcaata aacggtgttc aaaagcttgc
     1201 atctgcggta ataagtccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
     1261 cttattactt gatgcagtgc agctttttca caacctctta ataaacggtg ttcaaaagct
     1321 tgcatctgcg gtaataagcc caaccaaacg aaaaatatga ggaactattt ttgtttggct
     1381 gttcttatta cttgatgcag tgcagctttt tcacaacctc ttaataaacg gtgttcaaaa
     1441 gcttgtatct gcggtaataa gcccaaccaa acgaaaaata tgaggaacta tttttgtttg
     1501 gctgttctta ttacttgatg caggacagct ttttcacaat ctctcgataa acagtgttca
     1561 aaagccagtt tctcggtatt atgccaaatt tatgcaaaat gtggaaagcc attttcacaa
     1621 aatttttctt aatactcgaa actaaacgac tttttcacaa cctctttatt tcaaatctaa
     1681 ttctactgga caatgatcac tgcccataat gtccgtatgg atctttgcat cagctagtcc
     1741 tggctctaaa tctttgctcg tcaaaaagta atcgatccgc caaccagcat tgtttttcct
     1801 tgaattgaat cggtagttcc accaagagta gacaccttcc tgggttggat aaaaataacg
     1861 gaatgtatcg ataaacccgt tatccaacag tcgagaaaga gccgctcttt cttctggagt
     1921 gaatccagca tttttttgat tcgttttcca gtttttcaaa tcaatctttt gatgagcgac
     1981 atttagatcc ccacacacga tgactggttt ttgttttttt agattttcca aataattata
     2041 aaaggcttct tcccattcta atcggtaatc taaacgtttt aattcgcttt gagaatttgg
     2101 cgtgtaacag gttacaagaa agaaatcgga atattccaaa gtaatcagac ggccttctgt
     2161 atcatggata tcgataccca ttccgtaagt ggcattcaat gctggttttt tagcaaaaat
     2221 cgctgtgccg gaatagcctt ttcgttccgc gtaattccaa tattgataat aacctggaag
     2281 atctaaatca atttgtcctg cttggagttt cgtttcctgc agacagaaaa aatcggcgtc
     2341 taactcatga aaaacttcta aaaaattttt atttacgatg gcacgaagcc cgtttacgtt
     2401 ccaagagata aatttcaata aaagcacact ccttcgaatt aatttcctta cctttgcgat
     2461 actattgtac ctcattttag taaaaaatgg tgtaagatga aagtaactaa atgatccagg
     2521 aggaaagtag atgaagattg gtgtaatcgg tgtagggaat atcgcagaaa aagcatattt
     2581 accaacttat gcgaaaaaac aaggaacact tgatttttat tttgcaacaa gaaataaaga
     2641 aacaaaagaa agaattcaaa aagagtacgg gttccaacat ctatatgaaa cgatcgatga
     2701 gcttttagaa gaaaaaatcg aagcatgtat gatccacgca gcaacaaaag tacattatga
     2761 attagccaaa aaatgtctag aaaatggtat ccatgtttat attgacaaac cactgagtgt
     2821 ccatctaaat gaagttcgtg aattgcaaga attagctgat aagaaccaaa taattttgat
     2881 gatcggtttc aaccggcgct ttgctcctat ggtagaagaa ttgaaacgta tcccagataa
     2941 gcggttgatc cacctgcaaa aaaatcgaat agcggcaaaa gaaacgacag agttcgtcct
     3001 ttatgatttg tttttacatc ttattgatac agctgtgtat ttattagatg aacccatttt
     3061 gagaacaaaa taccagataa gagaaacaaa ggaatttttg gaatatgcca ttttacaatt
     3121 agaaacacaa aatcaaacgg ctattttaac gatggattta gcaagtggcg ctaatataga
     3181 aaaatatcaa gtaacaagta aaaatggtac ttatgaagta aacgatctga cccagcttgt
     3241 cattcaagat gctgatggaa aaaaagtgaa agaatttggc gactggacaa acacgctagt
     3301 caaacgcggg tttgagccaa tggtagaggc cttcttttct cagatccgtt taggtaaatc
     3361 ggaaacagaa ttgcttaaac aaaaagaagt tgtaagaagt catgaattat gtgaggaagt
     3421 gcttaaagat cactaccgtc atcaactttg aacaagactg gcaagattgt attaagcgtg
     3481 atagaataat gaggacgttt gagaaaagaa aggaaaatcc tcagtgaata aagaagaaat
     3541 agttgaaagc ttagccacga ttactatatt gtttgatgaa ccattgatga actatacgtt
     3601 tacgaaaaca ggtggaccag ctgatgtcct tgcattcccg aaaaaacaag aagaagtaaa
     3661 gcaaatcatc gattattgcc gaatccatga tataccttgg atggtattag gaaatgcaag
     3721 caatctgatc gttcaagatg gcgggattcg cggtgtagtc atcatgctga ctgaaatgaa
     3781 acagattcat gtcaaaggaa cgatggtcat tgcggaagca ggtgcctcac tgattgatac
     3841 gacttatgca gctttggcag aatcattgac tggatttgaa tttgcttgcg gcatcccggg
     3901 aagtgtcggt ggagcggttt atatgaatgc aggtgcgtat ggcggagaga tcaaagatgt
     3961 ttttgctgaa gtggatcttt tgcttgaaga tggaacattg aaaactttga caaaagacga
     4021 aatggtattt tcttatcgtc acagtaaagt ccaagaatta cgtgctatcg tattggaagc
     4081 tcgattcagt ttgcaaacag gggattatga agcaatcaaa gcaagaatgg atgagttgac
     4141 cgaattgcgt caatcaaaac agccattaga atacccttca tgcggaagtg tctttaagcg
     4201 cccagtagga cactatacag gtcagctgat ccaacaagca ggattgcaag gcctgaaatg
     4261 gggcggtgca caagtctctg aaaaacatgc tggattcatc gtcaatatcg atcatgcgac
     4321 agcgacagat tatattgaat tgattgcgca catccaagaa gtaatcaaag aaaaatttga
     4381 tgtgtcttta gaaacagaag tgcggatcat tggagaaaaa caagcaatat cagaaacaag
     4441 cagaagttta gccaattaat aagtaatgga acttaaaata agtaaaagag caggacggtg
     4501 gccgtcctgc tcttttttgt ggttgtttac gcaaatggcg caaatcttgt acattttaac
     4561 acaataaaac aaaaaatata gaatatttcc tcataaattt ttccattttt tgcaatattc
     4621 tcaaaaaaaa gataaaaaat gagaataact gatgaaaaaa caagcataat ccatcataaa
     4681 atcagtatag gtgaaaagat gaactttcat ctgcatatca agtaattggt atgaggcatt
     4741 ttttgaggtg taggatatga agttggatta ccttatagaa gaggaagata gagaaaaact
     4801 gctgctagct agatatttag aatttgagaa aaagaattat gtagtgctgc ctaagttata
     4861 tgaaacatta ggtttgtcta aatttaaaac aagaaattat atcatagaat taaataaaga
     4921 atttactgaa ttcaaaacaa agccagagat ccaaatttca gaagaagcag agatccatgt
     4981 cgaacgtctg aatttgacgg atatcaaaaa tttaagaaga atctactttc aacgatcaaa
     5041 aattgctcga ctgttaacgg agatcgtgga ttcaaaagta acgatcgaaa aatttgcaga
     5101 gaatcatttt ttaagcatat caagagccta tgtaaaaaga agagaattag tctcttttct
     5161 tgaacagcat caaataaagt taaagaaaaa catattagtt ggggaagaaa taaacatacg
     5221 gaacgtatta tttggggtat atttcggggt ctttaatggt ttttctatgc catttagaga
     5281 aagcgtcatg aatgaagcaa agagtatcgt gcaatatttt gaatatctgt tcaatttaaa
     5341 actgtctgca acaaaacgaa tcaaattcaa tttgctaacc actattagtt tgttgagggc
     5401 ctatgggaat caaccaatcg gaaaacgttt cttttcagat gaatacttta agagctcaga
     5461 aggaatgaag atcatcaagg atttatcaca tatttctttt cttcatatcc ctaaagagca
     5521 gatcattgat gagatttctt atatcatgtt gtttttgatc acggaagaag aatttgaatt
     5581 agatggcgca tttggacttg cggaagctca ttttcatgat atccacatga ttagtcaaaa
     5641 aatgtcagtg gaattgattg atcaattacc attttcgatt gaaatctctg aagaagaaag
     5701 actatatata cgaaaagaac ttcgtatggg tatgatgcat gtcaatcgaa aaaattggat
     5761 attcgacttt gagatgacta gctttacgtc taaaaagcag ctacagtttt tcttcgagac
     5821 gtatccaata ttcagtacag caatccaaaa ggtcatacaa gagaataaac gctatttctt
     5881 gggaaaagat gaacgtttca tgattcgctt attttatgat tatctctttt tattggtcgg
     5941 tatttgtccg gtatcatatt ttgaagcacc gattcatgtc tgtattgatt tttcgcaagg
     6001 atctgcttat accgattaca ttatttctca agtgaaagga tttaaaaatt acaatattgt
     6061 gattgagagt cgaatcactt ctcgtacgaa tatttattta tctgattgtt tacttgaaaa
     6121 atttaataaa gaacagataa tttggaaaaa tccaccgact ccagatgact gggaatattt
     6181 tggaaatacc atcatcagag aaaaaaatag acatttaact gaaaaaatca gctagtattt
     6241 atggatggca gctttgtctc acatatttat tagctgattt atgttttttt caaagaaaag
     6301 gtaactagat ataactagat ataactagat atattactta acaaaattaa aaagtacata
     6361 agttattatt ccgattttct tgaaacaata aaaaatcaaa aaaaatactc tcctaatagt
     6421 ccatcttctt ttgattttat ttatataatg aaatcatctt atgaaaatta ctacttcatg
     6481 tttagacaag agtagggagg actaagaata aatgtgttca aaaagacgtt caaaacaact
     6541 aaataaacac gcttgtcgtt tagataagga acggtcgtgt tctgaaaaac actacgttaa
     6601 gtttatgatg cgcacgatgc tgatcagttt atctgtaggt gtctatatgc atgtacccgt
     6661 tcaggcaacg acacaaacca tcaaggtcgt acgtacctct gtttctgaga ccactgattc
     6721 ccctatcact attactcata ctttcactga tgcaaatgga aaacagctac ttcctgcatt
     6781 gacttcctat aataaagtca gttatcctcc aacgataaac cactactggt taattcctaa
     6841 caaaacatta tggacatcgg aaaccggaag cagtgtgccg ctttctatct tttccgatat
     6901 gactgatcct acaaaaggtg atcagttaaa acagatcact gatattttga atcatgcttt
     6961 gacagatata aaggacggtg gaagcgcaca agtttcttat gtatatgaaa aagattattc
     7021 acagtttgaa agaaagaaaa tcgatgttct ggcagggaca aaagtttcga tatctgatct
     7081 ggtagaaagg ctgaagaacg cagatggaac acaaggagat atgtccaaag taatttcaaa
     7141 caatgggaat atcgtggata cgactaatac aggaacatct actatttcct tgtcctattt
     7201 tgatccaatt gctttaaaaa caatgactgc atctgctgtg atccatgttc gtgctgatca
     7261 aacagctatc aaaggaaaag atcttcactt aagaacagga gagagttacg cccctaaaga
     7321 tcttgttgaa tatgtaacaa aaagcgatgg aacttcagga aataaagaag aaatcaaaat
     7381 gaacgggaca gtagatacta caactccggg agaatatccc gttgtattaa cttatataga
     7441 ctcgaaaaca tcaaatgtgg cagaaacaac agctgtagtt tatgtagaag catctgctgc
     7501 ttcatcgact gtcatcactc attttataga tagtcaaaca aataaagaac ttcaagcaga
     7561 aacacatatg agtagtgtaa atcgtattcc tgatttgcgc ctacctgctg aattttctga
     7621 tcgagtgcca aatatagatt tatcgactta tgaacaaaat gggaaagtca cttctatttc
     7681 tgattggatg caagcaaacg gattagacag aaaaagtcac tggtcggaga tccttgaagt
     7741 acttcactca gaactgatga ataataggaa gcaatcagga ggggaaatta cctttactta
     7801 tgtttatata ccagacaatc gttcattaga aggtcatgat cttattgttc atccaaacga
     7861 aaaaataaac aaagaagagt tgatccaatc agcaacaaat tccttaggta aacccgtacc
     7921 gttaaaggat gtatcggtta caatcaatga agtaccattg gaggatggtt atttgaccaa
     7981 acaaacaggt gattttacga ttatgtatag ttatcatgac ccgtattcac tccaagaact
     8041 ccatgctcag tcactattac atgttttacc attaaacaaa cagatagatc aaaaagaaca
     8101 aaaaagacaa gaagaaagct tgtcagaaaa aaaacaacaa atggctcatg cagtttctcc
     8161 aacagggaaa gtaaaacaaa caagaagcct agagttagca agaatatcta gaaaaatctc
     8221 agaatctaat aaaacgataa ggcaagaagc tcatttggcc ccacaagttt ctggattgga
     8281 aaatgaaatt ttagagccta aaataaaaca acaatcagaa aaagaatttg ttgctaaaca
     8341 gtcggaagat cagaaggaat cagatgacca gaaggaaacg aagaaaaaac gcttgtcaac
     8401 tattgattcc accgatgagc atctgcagcc gcaagtacca cttcctagtc cgggaacacc
     8461 cgcacctggt ggaggtattc cttcttctgc tttaggagat ttcatgcgag gtatggcagg
     8521 aacatgggta tacgctaatc gaaatgaatt tgacgcagat gactggttct gatttatatc
     8581 tctatctgtt atagtcaaat aagtagctga gtcatatgtt atttggcata atggagacga
     8641 ggagggatag aaaaatgtta gataaaacgt taccttacgc agaaatctgg atgacaagac
     8701 cacttcaaca aaatgttcca atcacgcctt tagcggaagg ttttgaattt cgattttatc
     8761 aaaaagaaga tgaatctgct tgggcggaga ttgagacttc cgttggggaa tttcaaacga
     8821 cagatgaagc acttgagtat tttcaacaaa actttgctcc ttatcctgaa gaacttgaaa
     8881 gaaggatgtt ttttgtagag acaaaaaaag gagaaaaagt agcaacctgt accgcttggc
     8941 gaaaaaaatc aagagagcaa gtgtatccgg tttttcattg gttagcagtg aaaccagatt
     9001 accaaggaaa agggctcgca aaagcattga ctgccagagt cttgcaagaa tttcctaccc
     9061 tccatacgga cggaccaatc tatcttcaca cacagacatg gagccatcag gcgattgctc
     9121 tttataaaaa aatgggtttt acttttattg ctgaaaatct agacggcaca cctaatccag
     9181 attatgaaaa agttatgcag gttttaagta aattagataa ataacgaaat ggtgaaataa
     9241 gtcctcagta ttgggataaa aaacgaaata gtcattcctc atatcttact tttttgagtt
     9301 aatactatcg gataactaat ggcatgccat acattcaaaa aagaaggggc agtgacaaaa
     9361 gtattgtcac tgccccttct cttgtttttt agcatgtttt atcgtttgat ttttttagga
     9421 ataaaattct tgatgaacgt aaatttttgg taatcgtttc gactatagag gtaaccaaat
     9481 acactgaaaa caactgctcc aataaaatta acaaataaat ccttcatagt atccatgatt
     9541 cccacgtcta agtaaccatt ttggatgatt gttttatctg ttttaccatc tgcattttga
     9601 ctttcaatga ttgtccgagt gatatctgga acatgataga cagtgtttcc gtctttactt
     9661 aactccacac tattgatttg ctggatgatc gtatcttttt gcatatcgaa ttgcagtaat
     9721 ttatctgctg aaaattccac aaactcccac ataacaccaa ctgtcatcga aaaacagaag
     9781 gaaacaactg ctagtaataa aggagtcaag ttcatggttt ttgtattttt attgagcaaa
     9841 taaacaaggg aaaagccgac acccccagct aaaaatccgc tgaatgcatg tagaaaagta
     9901 tcccacattg aaaaatagcc ataaaaatca cttaactctc ctaaaactgt tgaactaaaa
     9961 ataaaagata acaaaatgat ttctagcata tttggaatct gtatctttaa tatcttttcc
    10021 agaaaataag ggatttcaaa taaaattaat gtcagtaata aaagcattgt atttccccag
    10081 tgttggttcc agacctgatc cactagcata ataacgacga tgatacgtaa taaaatatag
    10141 attccgtttg tgattctacc tgcagttttt gtactgttcg taaaaaaggt tttcaaatga
    10201 ttcataaaat aactccttca tgtatatcct taattatact aagaagcgtg tgtttaccaa
    10261 agctatatga cttaaagaaa aacagagttg atattgtgaa aaaaatcact taatatagta
    10321 taaggagaaa agggggatac aaatgaaaaa attagttgct ggtttattaa cagccgttgc
    10381 acttcttgct atgagcgcat gcggcgcaga tcagaaaaat acagcgcaga caagttctag
    10441 tacgaaggaa tctgccgaag tatcatcagg cgcttctgaa caagtatata cagatccaaa
    10501 agaaatgaaa gaatcttatg atgtcgtgat tattggagct ggtggtgcag ggatggcagc
    10561 agctatcgaa gcaaaagatg ctgggttgaa tccagtaatt ttggaaaaga tgcctgttgc
    10621 cggaggaaac acatctaaat catcaagcgg tatgaatgct tcagaaacaa aatttcaaaa
    10681 agaagaagga atcaaagact cgaatgatgc cttctatgaa gaaacgctaa aaggtggtgg
    10741 aggaacgaat gataaagaaa tgcttcgata ctttgtcgat cactctgctg atgcggtcga
    10801 ttggctagat caaaacggta tcaaactgga caacttaacg atcactggag ggatgagtga
    10861 aaagaggaca catcgtccgt ctgatggctc ggctattggt ggatacttag tggaggggtt
    10921 attgaataac gtccatgagc gggaaatccc tatttttgtc aacgcagatg tgacagacat
    10981 taagaaaaat ggggaaacgg tcaatgaagt gactgtacat gttcaaggag aagaaccaaa
    11041 agaggtcacc ggaaaagcaa tcgtagttac cacaggcggt tttggtgcca gcaaagaatt
    11101 tattgaaaaa tatcgtcccg atttagctga ttatattacg acaaatcaag aaggatcaac
    11161 tggagatggt atcaccatga ttgaaaaatt aggtggacaa accgttgata tggataaaat
    11221 acaaattcat ccaactgtgc agcaggatga gggggtcttg attggtgagg ttgttcgcgg
    11281 agaaggagca atcttggtcg atcaagaggg gaaaagattt gtcaatgaaa tggatacaag
    11341 ggataaagta tctgcagcaa ttacagcatt gcctgaaaaa tcagcttatt tgatttttga
    11401 ccaaggcgtg cgtgatcgtg ccacagctat tgaattttat gaccaaaaag gatatgtcac
    11461 aactggaaag acaatggaag aattagcaga aaaaatcgat ttaccaaaag aaacactagc
    11521 aaaaacagtt gatggatgga atacggatgt tgcggagaaa aaagatgctc aattcaaccg
    11581 tgccacagcc atggaacatg acttatcgtc tccgaattat tatgcgatca aaatcgcacc
    11641 aggaatccat tacacgatgg gcggtgtgaa aatcaatacg aacactgaag tattagataa
    11701 agaaaaccag ccaattaggg ggttatatgc tgctggtgaa gtcacaggtg gcttacatgg
    11761 aaacaaccgg atcggtggaa attcagttgc ggagatcatt atttttgggc gacaagcggg
    11821 catacaagca gctcgatttg cttcggaaaa tgaatgaaat agaacgtaaa taaaaagagg
    11881 ttgtgaaatc agcgttttga gctaataccg agggtcagct gattgaacac tgtttagtga
    11941 agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaagta aaaacagctt
    12001 cttatgtttt taacttggtt ggacttatta ccgcagatgc aagcttttga acaccgttta
    12061 ttcggaaaaa gagcgtggga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt
    12121 ataaacggcg gaagcagaag aaattccttc ggaaataagc tgaaattcac aaaaatttga
    12181 aaagcaattt tcgtgaattt cttcttattt cttggagtta aacgcttctg tcccagcctc
    12241 ttttttgatc agcatgattt tattcagtta tcttcaaaat gatttttccg atgtttttgt
    12301 ttgcttccat atattcatgc gcttgtttga ctgcgctgaa ttggaatgta cggtcaatga
    12361 ttggccgaat cttgccattt tctagaaaag gcccagcttt ttgcataaaa gtttgtgcga
    12421 gctctgcttt atactgatca cttctaggtg tcaataatgt accaagcagt tggacacgtt
    12481 tcccaatcaa tacaccaagg tctatctcag gcacgatggt cccacctagc ataccaatca
    12541 agacccaacg tccatcaacg gctatagaag ctaaattctt ttcccagtaa gaagcgccga
    12601 tgaaatccaa aataacatcg actccccgac cttcagtgat ttttcgtacc tcttcttgaa
    12661 agtcttgttt tttatagttg atcaattcat ctgcaccaag ttctttacaa aaggcgagtt
    12721 tttcttctga tcctgcagta acgatgattt tcgcgtgaga aagctgtcta gctaattgga
    12781 tcgctgctgt tcccacacca cttgctcctg catggatcaa gacagtttcg ccttcttgta
    12841 attttccaag ccagtaaagt gtttgataag cagtcaaaaa aacttctgag attccagcag
    12901 cagaaacata gtctagcgta tcagggagaa ggattgcacg atcggcaggc atcacagcat
    12961 attcggcata accacctaga ttgactaatc cgtatacttt tgtcccagga gtaaaagtgg
    13021 agatatcact tttattctca actacctctc cagctacttc gactcccaaa atagggtagg
    13081 gaggtttcaa agagactgat tctcttttca taatatctgt tcgattgaca gctgctgtat
    13141 ggactttgat cagtaattcg ccttgttttg gtttaggaac aggcacttca atttgttcta
    13201 atacatctac agaaccagga tgcttgattg aaatggcacg catggatcta ttcctccttt
    13261 gcccattctt ggaatgctgt attcaactct ttgctacctt cagaaagtac tagagtcgta
    13321 cgagaaatat ccgtttcttc cactagagcg gcaagtacag cagcaactgt agagcgagtg
    13381 atcgtttttg atgccttctc attaggctta gttaattgaa tggaagtaac ttcttcatcg
    13441 ttagtcaatg tgactggttg aagaatgaca aaatctaagt tagttcgttt cagccattca
    13501 tctgcataat gtttagtaat ataataatct gtcatggact caggccattt tgctggttca
    13561 tctgcataaa cagcactgac catcagatag cgagagacat gcgcattttc agctgcgatc
    13621 actgttttga ttgcgccatc caagtctact tgcagaagat tctttccttg agagccggca
    13681 gcaaaaatca atacatcgat gcctttgaat gcttcggtca ttttttccac ggaccaagtt
    13741 aaatcaaaag agacatacga aacattctct ttttttacta atgtttggga agcaacatct
    13801 ctcacccctg caaaaatttc atgggaactt ggagctaaat cttttatcag gtgtcggcca
    13861 atttgtccgt ttgcacctac aacaaaaatt ttcatttgga tcatcctttc gacgtcaaat
    13921 ctttccattc aatagtaaca ccaaaagaga aaaaaatcag agatcggcta cgatgactat
    13981 cagttagtaa gagaagggtg cttgaagtat cttaacaaga cattgcatac tgctgcagtc
    14041 gcaagacctg cgacgattcc ttcaggtata ccacttgtaa aaataaccgc tagaatagct
    14101 gaataaacag catttaaatt tgttccgatg actttggcat aatcttgctg gaataaaaag
    14161 tagatcatat tcatcactaa aatcgtattg gtcaatgaac cggaaagtcc agctaagaat
    14221 aaagaaacgg aatctgcttt ttgtttcatt agtttgcgca gacctttata aacgaaataa
    14281 ggaaccaccc caatcaagat gcgaggaata aacgcgatca gcaaggcttt ccagcttcca
    14341 tgttctgttc cgatgactgg gataaacgga gaaaagacaa aagacaaagg tgtttggata
    14401 actgtgctac ggatcatgct gatcagtcca aatgtaccgc caagaaaagc acctagtctt
    14461 ggtcctaaaa cgatcgaagc aatgattaca ggaatatgca tggttgttgc attgattgga
    14521 ccgataggga taaaacctaa aggcgtaaca gctaataaaa tcaaaatagc taaaaacatc
    14581 gcagttaatg taaagttttt tgtatttttc ataagtactc cttttttcta agcatttctt
    14641 tcattaagag tcagcatgac ctgttcgatg atcgtctgaa tatcagctaa ggcacctttt
    14701 ccaaaatctc cacaagctaa aagagattct cttggttcaa tttcctgata gccgacagat
    14761 ctcaatgttt ccagattacg ctgagtgatc gggttttgat acatattcgt gttcatggca
    14821 ggggcaatca attttggtgt tcccactgga agcgccattg cgactgttga aagaagatcg
    14881 tctgcaatgc cgtttgctag cttgccgata atgtttgctg tagcaggcgc gatgagaaac
    14941 aagtcagctt gttttgctag ttcgatatga ttgattacct gcggatttga ttcttgcatg
    15001 acgtctgtat ggactgcgcg cttcgacaga gattgcaagg ttaatggcgt gataaaatgt
    15061 gtgctgcttt tagacataat cacatctacc tgggcaccta atttgacgaa ttgacttgta
    15121 agatcagcgc ttttgtaagc agaaatactt ccagtaacgc ctagtaagat ctttttcatt
    15181 ttgattcctc cagtctaatt ttctcagtaa tcaatgcagc aatctcagat ttactttgag
    15241 cttcttccac tgttccatct ttagataaga gatagccgtg gtgtgtctcg ccatggattt
    15301 ccgtcaaatc atttgcgaaa ataaaatcag ttttgttatt tatcagacta ttttttgcga
    15361 ctgccagaag agattccttt ggtacatcta ctaatagttt gaaaccaatg atttttgctt
    15421 ttggctgcca ttcacgtaga taagaaatga tttttggtgt tttcttcaaa acgagcacaa
    15481 gatgctcggt gttagaagag atctttgaaa cagtggtatc tgtctgttca ttttccgcaa
    15541 accaattgtc cagttcttct tgcgttgaag aagtgggtag gttttttgcc agctgttctt
    15601 ctgaaaaact aaaagcagga gtaaaatcac tgactgccat actgtggata atcgcatcgt
    15661 agtgttcttt tttcgttagt tcttctaatt gtaaaaataa gtccagtgca gattcgatcg
    15721 tataaatcgt tatattggag tgtcttttcg gtttcagtgc ttgtttcgta gtcacgtaat
    15781 cgattgtggt atccggattg gcagctaaat aatcagctaa tgcttgccct aatcgtcctg
    15841 tcgaatgatt tgtaatagca cgtacttgat cgattttttc agtcgttcct ccagctgtga
    15901 ttagaatctt catttatggt cacctacatt tactagtgtt cttccttggt gtgttccagc
    15961 aagtagttta gatgctgttt caggcaattg atccagagta atctcttgga cgaccagttc
    16021 atctgcaatc tgcaaatccg ccatctgttg ccataaagaa agacgttgat ctattggtac
    16081 attgacagaa tcaattccaa tcaactggat gtttcgcaga ataaaaggca agacagtcgc
    16141 gtttagtttg attcccccag cattgccaca taaagctact gcaccgttat aagagatcaa
    16201 tggaagaaga ctgcttagct gctcgccacc tactgtgtcg atgacatagt cgatttgctg
    16261 ttttcctaaa gcttttgttg tctctggcag aaattcatca ggtcgtacga cttgcaaagc
    16321 acctagactt tttagccatg tgacagactc ttcttttctg gaaaaggcaa tgattgactg
    16381 gaatcctagt ttatgtaaca gggctatcgc tgtactgcct acaccgccag aagcaccagt
    16441 aacgacaact tttttatcgg aggtcatacc ttgacggatt aaagcgttca cacacaacat
    16501 agcagtaaac cctgctgtac caaggatcat cgcttgacgc agcgtcatat tttttggtag
    16561 aggaactaac cagtctcctg gtactttttg atattggcta taaccgcctg gatgggtgac
    16621 gccaagccca aaaccagtag caagtacaag atcttctttt gaaaaacgtg gattgttact
    16681 ttggacgaca gttccggata aatcaatccc tggagtcatc ggatatgttc gtatcacctg
    16741 accagactcg ctcattgcca atgcatcttt ataattgaca tcagaatagg caatttttac
    16801 caagacttca ttttcagcca actcagtaaa tggcagatca ctgattgctg tatggaaaac
    16861 aggttccttt attacctgaa aagatcgaaa agcgttcatt cgtgagacac tccttttaaa
    16921 attttctttt atcataccat aaagcctact cttttattct tgttcttccg taaatttaga
    16981 caaaaattag gtgaaaaata aagaatcaaa caactagaat ttattccaaa agataaagaa
    17041 atattcagca aaaaaaattt tacaaagggt tgcctttttc aataaatttc gtatactaat
    17101 acgagtggta gtaaacagga agtttactat tagaaaactt cgattcggag gaaattatgg
    17161 aaattggtga gaagttaaga aatttgcgca tccaaaagaa tttgacacaa gaagaactag
    17221 gcgaacgcac agatttgtca aaagggtata tttcccagtt ggaacgagat ttaagttcac
    17281 catcaatgga aacattcttt tctattcttg aagtcttagg tgtgactcca gaagaatttt
    17341 ttcatcaaga agcagcgaat ctgcaagtgg tttattcaaa agaagatcat acggtttatt
    17401 tcgatgaaga aaacgggtat gaattggaat ggctcgtggc tgattcgaat gaaaaagaaa
    17461 tggagcctgt cttgctgact tttgaaggtg caggagaata taagacgttt gaaccttcat
    17521 tatctgaaac gtttattttt gtattagaag gggaattaga gttaagctta ggagagacgg
    17581 tctatgtggc aaaagcagga cagtcgatct attatcaagc gacaaaacaa catcaattaa
    17641 ggaatcatct caaaaaacga acaaaagtat tgattgttgc aacgcaatca tatttgtaat
    17701 atagggggta aaaacgtggg gaaaccaatc atttcatttg atcaagtagt gaaacgctat
    17761 gatgatgaaa cagtattaaa aaaagtaagt tttgagatcg aacaaggaaa attttataca
    17821 ctgcttggtc cttccggctg tggtaaaaca acgatcctac gtattatagc tggattcacc
    17881 gaagctacag aaggggacat ctattttgaa gggaaacggt tgaatgattt gccagctaat
    17941 aagcgacaag taaatacagt cttccaagat tatgcacttt ttcctcatat gaatgttttt
    18001 gataacgttg cttttggatt gaagatcaag aaaatgtcca aatcagatat cgaaaagaag
    18061 gtaaaagatg ctttgcgtat ggtccagctt cctggatacg aaaaacggga gattagtgaa
    18121 atgtctggtg gtcaaagaca gcgtgtagcg attgcacgag caattgtaaa tgaaccgaaa
    18181 gtattgctat tagatgaacc gttatcggcg cttgatttga aactacgtac agatatgcag
    18241 tatgaactgc gggatctgca acaacgatta ggaatcacgt ttatttttgt tactcatgac
    18301 caagaagaag cgttggcaat gagtgatgag atttttgtca tgaataaagg gaaaatcgtc
    18361 caaagtggta cacccgtaga tatttatgat gagccaatca atcatttcgt tgctgatttt
    18421 gttggggaga gcaatatcgt ggacggtgta atgatcgaag ataatctagt tgagtttgtt
    18481 ggtaagcaat ttgaatgtgt cgatggcggg atgcgtctaa atgaaccagt tgaagttgtt
    18541 ttacgcccag aggatttgac gatcacgaca ccagataaag gaaaactagt cgtaacggtc
    18601 gatactcagc tgtttcgtgg tgtccattat gaaatcatct gttatgatga acagcaaaat
    18661 gaatggatgg tccattcaac gaaaaaagct aaagaaggaa gcaaagtagg attggccttt
    18721 gagccagaag atatccatgt catgcgtttc aacgagtctg aagaagaatt cgatgcacgt
    18781 cttgacagct acgaagaata ggggggacaa gaatgaaaac aatgcgcagg atctattccg
    18841 tcccttatat tttgtggctc ggtttattcg tgatcgcgcc tgtattaatg atcatttatc
    18901 aatctttttt tgatatgaat gggcaattca cgctgaataa ttatctatct tattttacgt
    18961 cggggacatc gctttctatg acgatcaatt ctgtatggta tgcatttttg atcacgtttt
    19021 tcacgctgat catcagttac ccgactgctt attttttaac aaaactgaag cacaagcaat
    19081 tatggctgat gctgattatt ttgcctacat gggtgaatct gctgcttaaa gcttacgcat
    19141 ttattgggat tttcagtgtg cacggcagcg tgaatcattt ctttgatttt atcgggatcg
    19201 ggccgaaaca gatattattc acagacttca gttttctgtt cgtggcaacg tatatcgaga
    19261 ttccatttat gattatgcca attttcaatg cattggaaga gatgaatcct tctttgatca
    19321 gtgctagtag agatttaggc gcgaatagct ttgagacctt ccgacgagtg atttttccgt
    19381 tatcattaaa tggtgtgaaa agtggtgtcc aagcagtatt tattccttct ctttctttat
    19441 tcatgctgac tcgtctgatt ggcggaaatc gggtgatcac tttaggaacg gctatcgaac
    19501 agcatttctt ggttacacaa aactggggga tgggttctac aatcggtgtg attttaatca
    19561 ttgctatgtt catcgtgatg ctcctgacag gcgagaagaa aaaaggaggt cgctcataaa
    19621 tgaaaaaatt taaatggtcg tatctttatt tagggatcgt ctttttattc ttatatgcac
    19681 cgatcttcta tctgattttt tattccttca atgaaggagg aacgatgaac agctttacag
    19741 gatttacttg ggaaaattat gaagctgttt ttgaagatac acgtttgatc attatcgtat
    19801 tgaatacgtt catgctggct ttcttatctg cattgatcgc cacgatcatc gggacatttg
    19861 gggctatggg aatctattac acgaaaaaac gacgtgcacg gacagcgttg ttgagcatga
    19921 ataacatctt attagtctct cctgatgtta ttatcggtgc tagtttcttg attttcttta
    19981 cactactagg attcggatta ggttttcaga gtgtattgct ttcccatatt gctttcagca
    20041 ttccaatcgt cgtcttgatg gtcttgccaa aattgcagga aatgaacgat tcaatggtcg
    20101 atgcggctag agaccttggt gctaataatt tccaagtagt caaaaatatc attttgcctt
    20161 atctttctcc aggaatcata gctggatatt ttatggcatt tacctattct cttgatgatt
    20221 ttgcggttac atttttcgtc acaggaaatg gctttacgac gctctcagta gagatctatt
    20281 ctcgtgcaag acaaggaatc agtttagaaa tcaatgcgct aagtgcgtta gttttcttgt
    20341 tctccatgat attggtcatt ggatactact ttattagtaa agaaaaccgt ccaaaacggc
    20401 tgaagaaaaa cagaagagta cgcgaggggg tggcacggct gcgatgaaaa aattacaatc
    20461 tctgttttta ggtattctca tcattattgc ggtgctgctg cttggtgttc gtcaacttga
    20521 aaaaacaagt ggcatgagtg gagcaaaggt acttaccatc tataactggg gagattatat
    20581 cgatcctagt ttgatcacga aatttgaaaa agaatatgga tacaaagtga attatgaaac
    20641 atttgattct aatgaagcga tgttcacaaa aatccagcaa ggtgggacaa actacgacat
    20701 cgctatacca agtgaatata tgatccaaaa aatgatcaaa gaaaaactag tacttccatt
    20761 agatcattcg aagatcaaag gattaaataa tattgatcca cgatttttag acttggattt
    20821 tgatccagat aatcagtatt cgattccata tttttgggga actttaggga tcgtatataa
    20881 cgataaattc tttgatgcaa atcaaattaa gcattgggat gatttgtgga agccggaatt
    20941 aaaagacagt ttgatgctga ttgacggtgc acgagaagtt atggggcttt ctttgaatag
    21001 tttaggctat tctttgaata gtaagaatat ggcacagctg acagaagcag caaataaatt
    21061 gaatcattta acaccaaatg tcaaagcaat cgtggcagat gagatcaaaa tgtatatgat
    21121 caatgaagaa gcctccgtgg cagtgacatt ttctggcgaa gctgccgata tgatgtggga
    21181 aaatgaacat ttacattatg tgatcccacc agaaggatcc aatctttggt ttgataatat
    21241 cgttatgcca aaaaccgtga aaaataaaga aggtgcctat gatttcatca actttatgct
    21301 agaaccggaa aatgcggcac aaaatgcaga gtatattggt tattctacac caaatgaaga
    21361 agcaaaagcg ttattgccaa aagacatttc gtcggacgaa caattctatc catctgatga
    21421 cacgatctcc catcttgaag tctacaaaga tctaggatcg aaatacctag gcatctataa
    21481 tgacctattc ttagaattca agatgtatag gagataatgg caaaactcag taagaatggg
    21541 gacttatcaa cgttctaaag agctaaaaat cgtccttttt ctatctgagt tttccaatct
    21601 tctaccaaaa gcggatgaaa cgccgctttt tttgtgttat tttatttttt ctaccacgtt
    21661 tttctaccag agaaaagtgg acaaaaccat tgtaaaatag ggcccgagac gggctagtaa
    21721 atctaagatt gaaaaagtta cagaaggata aagtaattgg aaactatgca aatatactta
    21781 acccttctgt aatctattaa taaaactaat atatgaatcc agttcaatat ttgtagct
//
LOCUS       DASHRQ010000045        21535 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.045, whole genome shotgun sequence.
ACCESSION   DASHRQ010000045 DASHRQ010000000
VERSION     DASHRQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.045"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(238..308)
                     /locus_tag="U2M88_001842"
     tRNA            complement(238..308)
                     /locus_tag="U2M88_001842"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(274..276),aa:Gly,seq:ccc)
     gene            425..1177
                     /locus_tag="U2M88_001843"
     CDS             425..1177
                     /locus_tag="U2M88_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEM8374225.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            1193..1672
                     /locus_tag="U2M88_001844"
     CDS             1193..1672
                     /locus_tag="U2M88_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEM8374226.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(1923..3503)
                     /locus_tag="U2M88_001845"
     CDS             complement(1923..3503)
                     /locus_tag="U2M88_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEM8374227.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(3496..4238)
                     /locus_tag="U2M88_001846"
                     /pseudo
     CDS             complement(3496..4238)
                     /locus_tag="U2M88_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(4414..5190)
                     /locus_tag="U2M88_001847"
     CDS             complement(4414..5190)
                     /locus_tag="U2M88_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8374228.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(5438..6274)
                     /locus_tag="U2M88_001848"
     CDS             complement(5438..6274)
                     /locus_tag="U2M88_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEM8374229.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(6285..7214)
                     /locus_tag="U2M88_001849"
     CDS             complement(6285..7214)
                     /locus_tag="U2M88_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEM8374230.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            7406..7759
                     /locus_tag="U2M88_001850"
     CDS             7406..7759
                     /locus_tag="U2M88_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEM8374231.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(7833..8489)
                     /locus_tag="U2M88_001851"
     CDS             complement(7833..8489)
                     /locus_tag="U2M88_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEM8374232.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            8715..10649
                     /locus_tag="U2M88_001852"
     CDS             8715..10649
                     /locus_tag="U2M88_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEM8374233.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(10735..11958)
                     /locus_tag="U2M88_001853"
     CDS             complement(10735..11958)
                     /locus_tag="U2M88_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEM8374234.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(11952..13850)
                     /locus_tag="U2M88_001854"
     CDS             complement(11952..13850)
                     /locus_tag="U2M88_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEM8374235.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(13844..14962)
                     /locus_tag="U2M88_001855"
     CDS             complement(13844..14962)
                     /locus_tag="U2M88_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEM8374236.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(15045..15878)
                     /locus_tag="U2M88_001856"
     CDS             complement(15045..15878)
                     /locus_tag="U2M88_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM8374237.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(15871..16788)
                     /locus_tag="U2M88_001857"
     CDS             complement(15871..16788)
                     /locus_tag="U2M88_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8374238.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(17015..18139)
                     /locus_tag="U2M88_001858"
     CDS             complement(17015..18139)
                     /locus_tag="U2M88_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEM8374239.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            18322..19230
                     /locus_tag="U2M88_001859"
     CDS             18322..19230
                     /locus_tag="U2M88_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8374240.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            19371..20984
                     /locus_tag="U2M88_001860"
     CDS             19371..20984
                     /locus_tag="U2M88_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEM8374241.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         6809 a         4168 c         3610 g         6948 t
ORIGIN      
        1 agacggattc atcgtctcta ctcaatcaca aaacactatt tacaaggtat ttattatcat
       61 tcaaaaaatt tttcttaata ctcaaaacta aacggcttct gtacgacctc ttggtaaaca
      121 gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa gtcaaaaaca taagaagctt
      181 ttttgcttga ctattcttat tacttgatgc agggcagctt tttcacagcc tcttttaagc
      241 gggtgacggg aatcgaaccc gcgacatcag cttgggaagc tgggatttta ccactaaact
      301 acacccgcga tacaaaaaca gtatacgcct ctttcactcc ttcctcaagt ttttttattc
      361 tttacgcttc cgatatgttt tgtagtaaaa taaaactaat caatcaatta ttggagaaga
      421 tgctatgacg ggaaaagaat tagatctgtt gctggacact tgtttactcg ctgggaaaat
      481 catgatggaa agcaatgcgg agatgtatcg ggtagaagat acgatgagtc gaattgcttt
      541 agcttctgga aatttccgtt tggtcagcta tgtcactcaa accggtctct ttattggtct
      601 tgatcgtacc tcgaccatcc gaatggaaca aattaccaac cgttcgatta atttagaaaa
      661 agtagtaaaa gtaaatgatc tttcacgtaa atatgttgct tcagagctaa cattagaaga
      721 actttactgc gaactgcaag caatcgaccg agaccgttct ttctttcctg tctggttgca
      781 aatcgttagc gcggcagtga tcagtggaac aattatggtg ttatttggcg gaaagatgac
      841 cgatttacct gtgactttag tcattggagg attgggatac gcagcttatc tatatagttt
      901 gaaatttttt agaatcaaat ttttatcgga atttttatct tctttattga ttggcatagc
      961 cgctatctac agcgtacgtt ttggcttagg aagcaatcag gatctaatca ttatcggatg
     1021 tgtgatgcct ttagttcctg gagttcaaat tacgaacgct atccgtgatt tgctagctgg
     1081 acattatgtc tcaggagttt ctcgcggaac agaagcgatg atgactgcta cgatgatcgg
     1141 ttttgcgatt gcttttattt tccagctttt ttattaggaa aggaacacaa gtatggggac
     1201 gatatttatt cagttttctt ttagtttttt agcgactgcg gcatttgccg ttattacaaa
     1261 tgtacctagg cgttcattga tttgttgtgg tctttctggt gcttttggct ggatgatcta
     1321 ctggatctgt gttcaaatag gcggatctgc tgcttttggt tcattgcttg gttctttagg
     1381 agtagcattc atcagcgatc ttttctctaa acgtttgaaa atgcccgtaa cgattttcaa
     1441 tattcccggg atggttcctc tagtccctgg tggtttagct tatcaagcgg ttcgaaatct
     1501 cgttactgaa tcctatcgag aagcggctca ttatgcggtc caagcaatta tgattgctgg
     1561 agcaatcgct ttaggtcttg tattatcaga agtctttaac cataacatcc gtaatttcaa
     1621 agtaaagcgt gacgcaatca tgatgaaacg aaaaaaacgg ctttttaaat aaacgaagtg
     1681 taacaaagaa aaggttgtga aagagacgtt cagccttgag cgatgacaaa atatctcaca
     1741 aacattgcga caagtaaccg caggagcaat aagaagtaat tatgagtatt aaaaaaagtt
     1801 ttgtaaaaat agcttctcac atttttacat aaaattgact taatactgag aaactgactt
     1861 ttgaacaccc tctatctgta aaaaaggggc tgcgacatga tttttgtcac ggcccctctt
     1921 ctttattcaa attttttcca aaacgttcgt tgataataaa cgatccatag agcggaaagt
     1981 attagaacta aaccgatcac aggaatattc actaaccaaa atggataaaa caaagccaac
     2041 attccgtata agacgataag aataatactg atgatcaatg aacccatcat agttgcgact
     2101 aaacccaaac ttcctgaacc tctgttaaat aattggttaa tattggtcca gttcagcagc
     2161 agcaaccgat aatctctagc gaaataacgt agtgacaata agtagcagcc gaataaagaa
     2221 ccaaatagca agctagggat aaaaaacaaa ggcagctgaa aagacagtcc gcttaataat
     2281 gcgatcccac cagttaaaaa actctgcaat atccatccga atctgaactt ttcttttaaa
     2341 tattggttca tagagattgg caaagaacgg ataaacagaa aattttcttg atccaaagaa
     2401 ataagattgc tgataaaaga agtctgatta actgttaaga aagccaatgc gatccctacc
     2461 aggaaaacaa caccgattaa cttattatct aaatcagtta attttatttc gcctcctagg
     2521 gcaaaagcaa gaataaaaat gattggagtt aacaaggaag aagaaagaac ttgcataatc
     2581 aaattaggtt cttttactaa ttgagcattg taactaaata aaagctgatg cacattttgg
     2641 ttctttttat gggttctttt tattttgaac tcactgtttg aatcagtcag ttgctctgta
     2701 agctttggta ctatcagctg ttttacagca aggaaaaatc ccagagccgt gatagctagt
     2761 attccccaac ccaataaacc ttgttgagaa agtggctgac tggcagcatc atagaatggc
     2821 aataatatcg aaatcggatg ccgatctgtt aactcatttt caatagtccc cgtttgatga
     2881 ttcatccaca tgattccagc aaatgcgatc agcatcgagc tgccaagcaa gatactagaa
     2941 actgtttttc tatgtttttt aaacactgct gttcgagcca aaccaaaaac aatcaaacta
     3001 gccagacaaa aaagaataat caaaaataga gaaaacaaaa ggattgagat aatgatagta
     3061 agcaaaatcc ctagatcagc tcgccatccg gttaaaataa acatgactag aagcggaaaa
     3121 acaaaaggaa tgatggtcaa agcaactacc aaaatctttg ctgaaaaaac ctgttcttgt
     3181 ccaaaaggca gaggcaaaaa tacttgcagg tcatttcctt caaaaaagat attataaatg
     3241 actgatatac tttgagttaa acctaacata ccaaaaagtg ccacgtaata agtaaagaaa
     3301 cctggcaatt taacaaagtt catagcaaac atagcaaatc cgtaaatcag taaaaaaatc
     3361 agccctgaaa aaagattttg agacaaaaga tagatagtta atcgctttcc cttttttcct
     3421 ttctctcttg ctttattcgt gagctgtgga ttaacatata aaagattaac tcgaattaat
     3481 tctaataatt gtcgcttata catacggtcc atctcctgtc gcttgccttc cagccatttt
     3541 caaataaatc ttttctagcg tttcatcagg tgagccattc aataattcac taactgcccc
     3601 ctgataaatc aattcacctt tcttcaaaat agccagttta tcgcataact gttgtgcggt
     3661 atccaataca tgtgtagaaa aaatgactgt ttttcctttt gcggcatgag ccttcatcat
     3721 ttctttcaaa tcaaatgctg cttgaggatc aagtccttgc aaaggttcat ccaataccca
     3781 aatatctgga tctggcagca acgtgccgat caaaatcgtt ttttgacgca ttccatgtga
     3841 aaaacttgcg attgtttcat tttgatgact atacatatca aaaagcgctg tcaattctgc
     3901 taaacgcttt tctttttttg tgtgtctaat tcataggcag ctgaaatcaa gtcccaatat
     3961 tctccagccg tcaattgtaa aaaaatatct ggggaatcag gcacataacc gattttttgt
     4021 ttgatactca atcgattttc cgtcaatttc attccatcta ctaaaatcgt tcccgatgat
     4081 ggttcaataa tactaactaa acttttaatc gtagttgatt ttccggcacc attatgtcct
     4141 aaaaatccaa aaatttcccc ttgtcttatc gagagagaca agtcttttaa tgcttgtttt
     4201 ttcccgtaac ttttacttac attgatgaat tcaatcattt ctgtactcct tttgtttgtt
     4261 attacttttt attgtaccct tcgttattgg tggagtaaat gtcaaaaaga gtgcataaca
     4321 aaaaaggata gaaacgacta ggctgctact tgggttgttc tcttctacag gaaaacactc
     4381 ttctaacagg taagatatga gtctttgttt tctttaaaat gattcatttt ctatcttttc
     4441 ttgcataaaa ttctttagtt tatcaatact tctctttccc atctctagtt gttctccatt
     4501 tgcaaatgtt actatttcgt ttactctgct aatagatgta atgttttcag tattaatgat
     4561 ataagctttt aaatctttac ataaatactc atattcgagt gattttttat attttttaat
     4621 tgtgccgttt acgagaatct cttcatcttt caatactagg cctattttat ttttataacc
     4681 tggcatgctc attagataaa ggatttcatc gtaatggata tagtgatcgc tattaccata
     4741 acggacagtt gtccaccttt ttttcgtagc atgttgaaca ggatagtttt ctttttcttt
     4801 caataagtaa ctttggatgc tttttttatt tttttcataa gtgaattctt ttatcaaata
     4861 accactaggc gtaatctttc gattaataac agttattgct ttattcggat gagacgttag
     4921 ataaacaatt ttacactttt cattcttttc tctaagcatt tcgcctagtt cgataccgct
     4981 ataataggcg ttcaaatcga tgtctaggaa aaaaatatct gaggaataga tttcattatt
     5041 tttaatgaat tcaacaaatt gtataacgtt caatccctga acgatctcaa atttccaagg
     5101 acttaataaa attctggatt cttccaaaat ttctactagc attttttcaa aaaccaaact
     5161 atcatccaaa ataaatatac gcgttaccat aaaaagtacc tccactcgat attttactat
     5221 caaaatcatt tcatacaact tttcaacata catgttagga ataaattgta attctcttca
     5281 caatcttttc caacaccttt gaaaccgata tcaaatactt tcaggatgct gcttctaaaa
     5341 taaatatcaa acaaatgaat acatcatgca aaaaaagcca gacaaccgac gtccttgcgt
     5401 gtcagctgtc tgagctatgt ttatttattt tgttctttta aaaagaacga tacacatact
     5461 gatttttcgg agcagagact ttctcagcgt ttgtaacaac tacagatggg atttcacgct
     5521 tgaatggttg ataatagtct gattggatgg ttccttctat tttataccat tcatcatttt
     5581 taaccgttac tccttccggc atatgcgtca acaaaccaaa gactcctgaa tctgccacac
     5641 aatggataat gccgaatcgg aacaagaaga tatctgtatt gctatcgttc ggtgtttggt
     5701 aaacaaatcc ttcatagctg atttttttgc caatgaattc gctagggtaa ttataaatta
     5761 gctccataac ttctaaatag ttttcatctg tgatcttgat agtttgctta tccccatact
     5821 tttctttcaa ctcattcatt tggtctaagt agtctgattt gttgaaataa atactggtat
     5881 ccggctttaa gtattgtgtc tgcatctccg gatcccctac tgattcttta cttactggga
     5941 aattaaatcc tttggcttct acgatcgtag tgtcgagact aactgttgga aacaacgtcc
     6001 ccacgattac tggcaaagct agcagtacat aagcgattcc acgttgataa ggttttgaca
     6061 acccatggtc atgatcgtgc tcgtgtgcct tttcttctgc ttttccgttt ttgacccaca
     6121 aatacagctg aactaaagcc aaaagagcag ataaaatcat tgaaagaata gctaagtagc
     6181 gataatggac attgatatat tgatcgagtt tgccagaaac ttgcaagtac atcatcaatt
     6241 cgaaataccc agataaaatc aaaaaccgca gcatattttc cctcctaaat aaataatgtg
     6301 aatccaataa cgctaagggc aatcagtccg atcagcgata aaataaagcg tcctgtaaaa
     6361 tatcgtttca tcatcagtag atttttgata tcgaccattg ggccaaagac caaaaaggca
     6421 gctacaggtg ctgtgccgaa cagacttaac aaagaagatc ctataaacgc atctgcttct
     6481 gagcaaagag acatcgtaaa ggccaataac agcatgatga taatggctaa aatctttgtt
     6541 gagccgagcg ttagcataat tctagttggt aaataagtct gcatcgcagc tgcgattaat
     6601 ccgccaatga tcaaataacg acctgtatca aaaaattcgt cgattgaatg tgtcaacaca
     6661 tgcccagttt gatggatcag ctgatttttt ggttcattct tctcactttg ttcttcttca
     6721 tggacatgat gatggtgatt ttctgctact ttagacgtga ttttttcttt caatacagcg
     6781 tctttttgaa aataggcaag ccagacacca atcacaaggg caacaaaaaa gctgcctatc
     6841 acccgccagc ttgccatctg ccaagaattg ccaaatgcga taaaagtgga aaaaatcacg
     6901 attggattga tgatcggagc agtcagcata aaagcaaacg ctgtgtgaac tggtacgcct
     6961 tttttaacaa attgatgaac gatcggtacg atgccgcatt cacatgaagg aaagaaaaat
     7021 cctaagatcg aaccagtaaa aatagagaag atacgatttt caggaagcca tctcttcaca
     7081 cgttctgggg tcaaaaatac ttgtaaggcg cctgagataa gacagcctaa taatacgaaa
     7141 ggtaatgctt caataacgat cgataagaaa atcgtactca tctgcaaaac cgaatttgga
     7201 ataaaagaaa acatgagttt cctccttttt atttctagtt cattcgcttt gcaaatatac
     7261 gcttttattc aaaaaaaaca agttgctctg taaattttta cacaactctt attttttacc
     7321 aaagcacttc aattgacttt ttctgttatt cccattaaat taaggtctca tacatatcac
     7381 ttctatcata aaagggagaa taaaaatgaa acaaaccact aacttgactg aagttcaaga
     7441 catactgcaa cagtcatccg ttgccgtcat ttatctttcg atgcctaatt gcagtgtttg
     7501 tcacgctgtt cgtccacgac tagaggaact tctcacacat tatgatattc cggcacttca
     7561 tttggatgca tttgaaacac cagaagtagc tagtaaattt gaagtgctga ctgcgccagt
     7621 tgtcctgatt tttcatcaag gaaaagaagt ctttcggcaa gcacggttca tcgactttaa
     7681 aaaaatccgt tatctactag atgaactgac agcagaagat gattcccttt cttatgaaga
     7741 aatcttccga acaaaataat cgttgtccaa acagaaaaaa gcgattgaac aagtgaaagt
     7801 tttgccttca tttgttcaat cgctttttat ttttattctg ctgtattttc ggaatgcgca
     7861 tttgcgtcag aatgcaagaa atagatcaag gtttgcaatt cattcgtcaa atcaacattt
     7921 tggatcaata catctgacgg agcattcaat cgagcagcgg taaagttcaa gattcctttg
     7981 attcctgctt ccgttaactg attgactact tcttgtgctc tttcagcggg tatcgttaaa
     8041 attgctactt cgatttgttg gatcttgatt tgttctgaca tatcttccat tggataaacg
     8101 gggataccgt ctacgatgcg cccaacgatt tcctcattca catcaaaagc acaactgata
     8161 cgtatgctat tactttgatg gaatttgtat tttagtaaag cacttcccaa gttccctaca
     8221 ccgatcagcg caacatttgt caattgatcg tcgtttaatg tctttgcaaa aaagtgcata
     8281 agatcttcta catcataacc atacccacgt tttcctaatt cgccaaaata agaaaaatct
     8341 cgacggatcg ttgcactatc tacttgaacg gcttcactta attcagttga agacactttt
     8401 ttctttccag cattatttaa aatccttaaa tagcgataat acaaaggcag tcttttagct
     8461 gtcgcctttg gaataatatg atctttcacg aaatgacctc cacgtattgt ctaattacat
     8521 ctttgatttc tccatcatag cagtatttat aggaaaaaat caattccatt gctcagatat
     8581 tgtgaaattt tattctttat tcatttattt atcatttctg caaatctcgc agtataatgg
     8641 aatttatggt agactactaa ttgaaaaata gttttttatt tgaggagaat tcacaaagta
     8701 gaaaagagga ttccatgata ttattacaag ccaatcagat tgctcgccat tttggtgcag
     8761 aagtcttatt tgaaaacata catttagaaa tcagtacagg cgctcgtgtc gctttagttg
     8821 gcagaaatgg cgctggaaaa tcgactttat taaagatcat tgctggaatt gaagcacctg
     8881 atgaaggaaa gatcgctaaa aataaaacag cgactctcgg atacctcgct caggacacag
     8941 gacttgattc caatgaaaca gtttggaacg agatgctgaa agcttttgaa caagttagac
     9001 aaatggaaga gcggatgcgc gagctagaga ttttcattag cgaaggtacg ccagacacac
     9061 ctgcttatag ttcttattta aaagaatacg accggctgca acacgatttt tcagatcgaa
     9121 acggctatgg ttatgaaaat gaaattcgtt ccgtattgca tgggtttaaa ttcgattctt
     9181 cattttatga tcagaaaatc gatactttat caggcggaca gcgcacacgt ttagctttag
     9241 ctcgcatgct tttgcaacac cctgacattt tgattctaga cgaaccaacg aaccatttag
     9301 atatcgagac actttcctgg cttgagaatt atctgcaaag ctatactggt gcattattga
     9361 ttgtttccca tgaccgttat tttttagata aagtagtcaa tgaagtctat gaattaagca
     9421 gaagaaaaat gcgccattac aaaggtaact attcgaaata tctagatttg aaagcagaac
     9481 agttagcaag tgaatggaag gcttatgaaa agcagcaaac agaaatccat aaattagaag
     9541 acttcgttgc tcggaacctt gttcgcgcct ctaccacaaa acgagcgcaa agccgcagaa
     9601 agacattaga aaaaatggac cggctggatc gtcctcaagg ggatgaaaaa caagcaaact
     9661 ttctattcag tatagaaaag ccttctggaa atgtagtctt gcaaatcgaa gatgccgcta
     9721 tcggatatga taatgtcacc ttatctgaac caatcaatct agatatccgt cgtcaagaag
     9781 cgattgctct agtaggtccg aatggtatcg ggaaatcgac gttgctgaaa tcaatcattc
     9841 accagatccc ttttatccgt ggaaaagaaa cgctagggac aaatgtcacc atcggctatt
     9901 acgatcaagg tcaagctgat ctgcatacaa acaagacgat cctagcagaa ttgtgggatg
     9961 agcatcctac cactccagaa aaagagatcc gaagtgcttt aggtgctttc ttattttctg
    10021 gcgaagatgt agaaaaacca atccctctgc taagtggcgg tgaaaaagca cgggttgctt
    10081 tagccaagtt agcgatgaac aaagaaaatt ttttgatttt ggatgaacca acaaaccatt
    10141 tagatattga caacaaagaa gtattagaaa atgcattgat cgattatgac ggcacactgc
    10201 tttttgtttc ccatgaccga tatttcatca atcggatcgc gacaaaagtc gtcgaactct
    10261 ctgaaaatgg cagcaaagtc tatctaggag actatgatta ttatttagag aaaaagcagg
    10321 aagaagaaga aatcgccgaa ttgttggcac aagatgagcc agaatctgct cctgctccca
    10381 cgaacaaatc tgattattac caaagcaaag aacagcaaaa gcttatccgt agccttcaac
    10441 gaaaaattac tcaggttgaa gaagaaatgg cgcggatcga tgaatggatc gatcagttgg
    10501 aacaagaaat ggtcgcgcca gaaaacctgg aagatcacgt aaaactcaat gagctgaacc
    10561 aagatttaga agctgctaga caagatcaag aaacaaaatt gacggaatgg gaagaactaa
    10621 gcttggaatt agaagaatat gaaaattaac gaaaaaacca aaacagaaac cattcatgct
    10681 gttttggttt ttcttttttt attttatcaa acaaaaattc catatttacc tttttcacca
    10741 ataagaagac caattactag tcattctcac taaagcttct aaataatagt aatcacccca
    10801 aatacaaggt gtatttaccc cattattttt gggcttatta taaacagact gtgttaaaaa
    10861 gccatcttct tcttccgttg tagcatatct ttctttcaat tgtcgcataa ttttccaaac
    10921 agcagactgg tataagttct tttctgtttt gtctaataga cctgattcta ttaattctaa
    10981 taatccacaa accatgatca ccatactaga tgtatctttt tcttctctgc tagaatcatc
    11041 aaagatcaaa tcccaataac aaatggtatc ttctggcaac cgattcataa aataattggc
    11101 taatattttg cttaaatcaa aatatttttg ttctttcaaa taacgataac ttaatgcaaa
    11161 accgtaaatc ccccatgctt ggcctctagc ccaacaggaa ttatcactat aaccttgatg
    11221 cgtacttcca tatcttggtt ttccatcttt gacatccata taaaatgtat gataagttga
    11281 accatcttct cttactaaat aatctgctgc attttgaata tgagcctgag cagctttttt
    11341 atatttttga tttccagttt cttcactcgc ccaatacaat aatggaagat tcatgcaaca
    11401 atccatgatc attctacctt gttgtgaagg atcactagga tctccccaag cttgtataac
    11461 tcctgccttc tcccaaaaac gctgaagtaa tgtttctgca gcatcaattg aagcttcctt
    11521 catttttcga tttcccgtta ttttataatc tgctacggtt gataaagaat acaaaaaacc
    11581 aagatcgtga tgattagtga taatttgaca gtcaatcctg tggataaaat ctatatctgc
    11641 tttttcagct gtttgttgaa ataaaggatc acccgtcttc tcaaataaca accaattaat
    11701 tcctgtccaa aatccttctg tccaaccaat attgtctctt aatggataac gtccttttat
    11761 aggacactcg cttgggaatt gatactgact gttactgata cgtttgatta acctctccgt
    11821 gtgtctgcga caacgctcga tctcattatt ccattctttt tccattaatt ctttataaca
    11881 ttcatttatc catttttctg gatgtattat ctcttcaaac atcactttat caagcatttc
    11941 cccaccagcc tttagcataa ttttagtctt tttttattgt ttttattttg gatagttagc
    12001 ccggttgtcc catcaaaatc aattttggga cgattgttcc atacttctaa actataatcc
    12061 cgatcttgat gagcaaccac tcctgttccg agccaatggg ttccttttcc aaatgtaccc
    12121 tctaatccag gaataaaagt gttttcccat gtcatcaagt ttgtattggg caaacccata
    12181 atagctgtgg attttcgttt aatagtatcg tcacttaaac agataatacc tgaaaagcca
    12241 gcttcactca ccctatagtt ttccttgtct tcttctatga ttattccttc tttctcagga
    12301 gaacattcaa tagcaaaccc tgtctcatat agaacatatt ctgtatctaa ttctactttg
    12361 tgtacgcgaa tctgccagcc ttcaaaagga ataagccaag ttctgatttt tgcctgtaaa
    12421 gtaggttccc atgtagaggc aagtacctct ttcttacata caatgtctcg tacattattt
    12481 cgaacgatga actgtccata ggttttgatg ctctcattct taaaactggt cgtgatatat
    12541 gtgtccgctc gctgaatccc tagcattgaa tccattgcca tttctgcttt caattgattc
    12601 gctcgcggta tcgaaaaacc aaattgacta gaataagcaa attttaaata tttttcattg
    12661 gtcaatgtat ggaaatttgg ccctaattgt cccgcattca aaaaaaatgt atgtccatta
    12721 tcatgttgta tgagcaaatt ggcttctgaa agaagtttag ttccttcacg aataggcata
    12781 ggcatttctt tagccgtcca atactcatgg tcttcaggta gcgccaacaa taaaaaaatc
    12841 ttattcagcc ataatggaga taacgtacta ttatagggtt cagtcaatgt caattgaggg
    12901 taggtataac caatattcag gatacctttt tcatcaaata tttctttttg catccaccat
    12961 cttatattcc gattgactaa ccccttgatt tcacctaatt taaaaggcaa aagatcggcc
    13021 cacaacatag ctgaccaaaa gcttgaaact gcaaaacgat aaatcatgct tcgaccaaaa
    13081 ggcacatttg caccatcttc cgcaaaaaaa tgaatatagt ctctagaaaa atcaatagct
    13141 cggttcttat atatctggct aatgggcttt tctggttcta gtacgctata aatcaaacca
    13201 taataatgaa atgcaaaagg attataataa tcttggcgac ctcttgaact gtctcgatac
    13261 cagccatttt gaagataaca attttctata ttatcaaatg cttctttcag tgctgattct
    13321 ttaactggat aaccaaattt ttggataatt gttccgacaa ttatcttaaa aaattgccaa
    13381 ttaccatctg cacattcata ttcaaaaatt tgcgagaacc aatgaataat ctggcttttt
    13441 tctcctgtat ctaatttttt ccataataat tgttcatgat gatataacgc taataacatt
    13501 gcgggcattt ctaccatacg ctggtctctt tcatgtattt ctccccaata atcgggatgg
    13561 gaaggatctg ttccagcttt tattcctgat agaattaatt tcaaccatcg ttcatctaat
    13621 tggtcaattg cgggccccgc tccccataaa acccgagaga atccttccat tcctgcaatt
    13681 ttatttccat atcctacccc ttgatgtata taatcaattt gtgcgccact tgctcgataa
    13741 aacggctgga gtggttgatt tatcttttta aataagtcaa agtaatcctg tctggtcctt
    13801 aatagtggta ctggatgttt cattgtattt tctcctcttt ttactaccaa atagctaatt
    13861 ccgactgttc taatcgaagt aagccttcaa taaaaaaata atcagaatag atcagtggca
    13921 cattataagt cagttctcta tggtatgctt ctgctccgcc tccaatgatc ccatcttcat
    13981 ccatattcca atttgcatag tgcttttctg tcttttggag tatattgata gcagcttcat
    14041 gatacatttt tgatcgtaca gaggaaacat atcctgctaa ttccagaaga ccgcatgcag
    14101 cgcatactcc agcactggta tcaatattcg aatcttttac agcaaaatca atgagaggaa
    14161 tataaccagt tttttcgatt ttctgaataa aatttactgc tatcttttct gctatagcta
    14221 gatagtacgg attttttgtg taacgataac ttaacgcgaa tccatataaa gcccatgcat
    14281 ttcctctgct ccaacaagaa ttctcattgt atccttgccc gccaagtaac cgaatatatt
    14341 tccctgattc tgcatcaaat tctgcgatat gcccggttga tccatctttc ctcactagat
    14401 acttagctgt tgtatctgca tgctgccgag caattttaga gaacctcgga tcaccagttt
    14461 ccctcgaagc ccaatacagt aatggtaaat tcatcatact atcaataatc atccaaccag
    14521 gtttatggtc attccatgct actaaatagt tccctgaaat attatagcgt ccagcaagta
    14581 ccgtagctgc atgtaaccct agttgtcgac tatgtttgtt ttgagactgt tgataatgtg
    14641 caatagccga tggtaagaat aaaaaaccta catcatgatg aacgtctgaa aaatggtaca
    14701 gggcagattc taataattgt tcttgttgta ttgctgcctc tttgaacttt tctcgttttg
    14761 tcttgtgaaa gagctgccat aaaataccac tccaaaaacc attggtccac cagtcgattc
    14821 ctctgtcctt tattacgtct gtatattttc cgttgtaaga aatatatgga attttattcc
    14881 ccattctgtc taattccgca tctatctttt cagaagctac attcaaaatt tttcttgacc
    14941 attccttcgt ctgttcattc atggatatcc cgccattctt ttaattttta tgaatgtgga
    15001 gttgctccta gaacgaacta gatggttaac attcgttatt ttttttactt atttgcagct
    15061 ccaatgtcat tctccttaaa tttagactgc gcccacacat aaatcaagac cattgggatc
    15121 actgaagttg ctaaaattag cataattgcg ccataattaa ttggctcacc tgtactagaa
    15181 accgtcatat ttttgataat taagggaacc gtatatttat ctttatctga aagcatgact
    15241 aatggaaata gatattgatt ccaacttccc ataaagataa gtaaaccact agtaataatc
    15301 gtaggtgata aaatcggtac agcgattttc gtaaaaatca cccactcatt ggcaccatcg
    15361 attctggcag cctccatcag tgctgtagga aattgactgc tatattgtcg catcataaac
    15421 actgaagttg tactggcaat cgaagggatg atcacacctg cataagtatt cgataatcct
    15481 aaacgactca taatgtaaaa taaaggaatc aatgtggtaa aaaaaggcac catcctagat
    15541 agcataactg tattgaacaa cagtttattc cccataaaac gatactttgc taatgcataa
    15601 cccgccattg tagaactggc tgtagctgaa actgtactga ttacagcaat aacaatcgaa
    15661 ttccaaataa tcaagggata attatatctc gccatgatgg cttgccagtt ttttaatagc
    15721 atatccccaa acttgaaatt gattccgcca cttaaaattt cttgattact ttgcgttgaa
    15781 gctacaatca tatataaata tggcatcaaa aatattaagg caccaaacac aataatgacg
    15841 tacgtaaaaa ttctgctttg tatcttttgt ttagtcattt ttatcacctc cgaatttaaa
    15901 ttgaatagcc gataatataa cagatacaaa caccatcgtc caggcaatag cagaggcttt
    15961 tccaaaatca accatttcaa aacttgtttt atataaattg actcctacga ccaaagctga
    16021 attttctggt ccattggaag tcccaaagaa tatatttggg atattgaaat tacccaagcc
    16081 attgatagtg gctaaaactg tggccattaa aatgattggc tttagcatgg ggatcgttaa
    16141 taaaaagaat tgccgaaatt tcgatgctcc atctatttca gcactttcgt atatctcttt
    16201 agaaatccct tgaagaccag ctaaaaataa aatagtgtta tatcctacac taagccaaat
    16261 catcgtgatg atcattgata ttcgtatcca aaaaggattt gttaaccact gtacaccgtt
    16321 aattcctagc gcattcaaaa atccattgaa taacccagtc ggattgaaaa aagtcatgaa
    16381 tacggaagct acagccaccg ttgatgtaat ggttggaata taatagacag ttctaaaaaa
    16441 tccttttcct tttaactgtg ctgaattcaa taaaacagca aagtaaatag ctaatagcat
    16501 cgttattgga attgatcctg ccatgataat caaaacattc gtgtaagatt taataaacaa
    16561 tggatctcgg aagatagcta gataattgtc aaatccaaca aattcaaatg agtagccaat
    16621 tttcataaaa ctaaaagcaa acgagataat gatgggaatc aaaatcgtca caataaagaa
    16681 aataacaaaa ggtgtgacca atccataagc acttttttta tgagacgtat ctgttgcttt
    16741 tttcttgctg ttctttattg tcttgcttgg taacgaagat tgcatcatcg ttaagtcctc
    16801 ctaatatttt ttaatcacca gttaatttta cgccgtactt atgagcaaaa ttttctgctt
    16861 cttttagata tgaattttca aaatcacctg tttgccaata gttatatgca gcggtagtca
    16921 aataagacgt agcatctgaa taagtggctg gtaaaacagt atttgatgta ttttcattga
    16981 ttttattcaa tagttcatag tatttttgtc ctccttagta gtcaaacttt ttctcagctg
    17041 cttctgtttg ataggccaat gagttagatg tagccacgcc cagctccagt gcgttggcta
    17101 agcattcttc atctgtcagc ataaatgtaa gaaattgttg tgccacttgt ggatttttgg
    17161 catctttagg gacataaaaa cttgatcctc ctgatactgc accttttcct ggattttctt
    17221 ttgaaaaagg aatcagtgga gccatgcgcc atttgtcaga tgcatcagga aaattcaaag
    17281 acatattcgt agcataccag ccgccagcaa caaacattgc agattcttga aatgctttct
    17341 cagcatcttg tgcaccgtag aaatgtacaa tatctgagtc gatcatttct ttgattaact
    17401 ttgcagcatg tttcgcttct ttagtattca gacggatatt tccttcttca tcaagcaaag
    17461 attgattctg ctgggctagc atgttgataa acaattcttg gtccccagat tctggcaagg
    17521 caattccaaa actaccagtt ttttctttta atgcttttgc cacctggata tattcttccc
    17581 aagaactgat ttcttcaaaa tctacaccta tttcagcaaa tttttttggt tgataataac
    17641 aaagtgctgg ggtaaaatca ctagcccaag gaataatcgt ttcattactc gtttgttgac
    17701 gcaaaatatt caatttttga tcataaaact gatcgccata tttgtcataa aatccatatt
    17761 catcagcact ataaaaagca tcttgaaatt tttccagcac acctgctgct gaagtgtctg
    17821 ccatggagat aatatccggc atatcttcgt gtgttaccaa ttttggggtg atcatttgta
    17881 ctgttgctgt tcccgaagct acattcacaa tttttaagtc aatatttgct ccagttttgc
    17941 ggttatagat ttttgcagca ttttcaacat agtttgtcag tttgccgttt gcgacatata
    18001 cttctaatgt agtggctttt tttatcattg gatctgcgat gtttgctgtt acttcttcgt
    18061 tagtttttcc acatgcactt aagctgaata atagggtaat gtttagcaaa gctatcacca
    18121 atttgtatct ttttttcatg tctatccctt ctttcttttc aagttcttat ataaaacgct
    18181 ttcacttatg tatattaacg gaattagtaa cacaaatatt tatataaaaa aatattttac
    18241 ttgtcgtttt ggcttttcat ttttgttata atagtcatta attataaata gaaagctttt
    18301 tatataaaaa gagaaaggaa agtggttctt ttgttaacaa acaaccaatc ctccaatctt
    18361 gtcaaaaaaa cactaaccta ttccatagat acccatcttc atttatctgc tgctccctat
    18421 tttcgactac taataaatga aagcgctaat ttatatgctg aatttgcaca taaatctata
    18481 gaactagata atccttccct tttattcatt gataaacagc aagaagtcca aatcttttct
    18541 actcaaaagg aaacacaaac ttttatcatt atccagtttt attttgatga gcaattatta
    18601 gcacatatta cgggacaatc tttttctata gatcaagaaa taacatctat cattactttt
    18661 ctgaaaaatg aacagttcaa aattgattat ctaaaacata gtaagccagc tatttcctat
    18721 gatcaggaaa tcaattcttt tttcatacta tgccaacaga tgagcattca tttgctttcc
    18781 ttattagtta cttattgtct gcaaaaaatt caattgtctt ccttaccaag gcattatcaa
    18841 caggaacttg ctttattgac aataccttca aatgagaaag tcattcctaa aaaaaagcat
    18901 attctttatt cagatgaatt agttgatcca atcatacgat atttagaaga acatctttca
    18961 gaaaattttt tgcttgaaca attagcccaa atttttttta tcagtccagc cagcttaaaa
    19021 aaacatttta gaaaagtgaa tggaaaaagt gttattagct attttaaaga actaaaaatg
    19081 gaaaaagcaa aacgtctcgt gctagaaaat gaattaacct atacagaaat tgcagatata
    19141 ttaggatttc attcgatcca tcatttttct tcagctttta aaaaatatac agggttatcc
    19201 ccttcaaagt attatctatc cttaaaatag aaaaaaattt ttaggtttta tccacaaatt
    19261 atttctctat attttctgtc tattcaaaat aaacaataat tttccggcac aattatttgc
    19321 tgttttttac cataacatag tacgatcaac gtagaaaagg agatgttttt atgaaagttg
    19381 ttgtcattgg cggtgtagct ggcggtcctt catttgccac tcgtttccgt cgattgaatg
    19441 aagcgcacga aatcattatc tatgaacgcg gagaaaatat ttcttatgca agttgtgctt
    19501 tgccttatta tttaggaggt gtgatcactg accgtgactc gctgatcgaa cgtacaccag
    19561 aaatattgaa aacaaaaaac aacatcgatg tctttactaa acacgaagta acagcaatcg
    19621 atccttctac taagcgatta acagttaaag acctatccac aaatgaagaa ataaaaacag
    19681 attacgataa gttgatcatc tcttctggtg ctagaccaga ttatccggat attcccggag
    19741 tttttgaagc agaaaacggc tttgtcctcc gtagtgtgac ggatgcggat cgaatcaaat
    19801 cgttccttga agaaaaaaat ccacaacatg tcgtcattct tggtgcaggt gttatgggtc
    19861 tggaattagc tgagaatttc aagcatcgcg gcttagacgt gactttaatc gatcaattgc
    19921 cacaagtcgc tttcccttat gatccagaaa ttgctaattt agtttatgac aaattgctga
    19981 aagaaggatt agccgttcat ttagaaacaa gagttactga gatccgtgat aaaggtcgag
    20041 aaatagtatt atcagatggc tccgtccttt ctgctgatat gctaattttt gctgttggtg
    20101 tttctccgaa gaatgaagtg gtgaaagcaa ccggcataca attatcagat acaggtcaga
    20161 tcattgttga tgaccagtta caaaccaatc tgccggacat ctatgcgatt ggcgatatta
    20221 tcgaaacgac tagtgtagtg actggtcagc cgatccaaag tatgctttcc agtgcggcca
    20281 atcgtcaagg acatatgctg gcagatattt taaatggtac gcctatgcgc tatcgcggat
    20341 atattggtgc aggtgtcgca aaaatctttg atcatacagc aagttatgcg ggaatgacag
    20401 aacatgaatt aaaagcatca ggcataacaa attataaaac tgtttttatc actccctttg
    20461 accatgccta tttctatcca gaagctacaa gattaaatct aaagctgatt tttgatgcag
    20521 atagcggtcg tattttaggt ggacaagcat ttggagaaaa aggtgtcgat aaacggatgg
    20581 gagaactttc tgtagcaatc accggaaact tgacagtctt tgatttgcct gatttggagt
    20641 tgccttactc tccaccgtat tctactaccc gtgacccgtt gaatatagct ggttatgtcg
    20701 caatcaatca aatgacgaat atcgtagaaa cgatcaaagc aagtgatata cccgaaaacg
    20761 atttgaaaga agcgttcttt ttagatatac gtgaacctaa taaagcacct tctggcagta
    20821 tctcggcaac aaaaaatatt ccaatgaatg agttacgaga tcgaatcaat gaaatcccaa
    20881 aagataaaaa agtttacatt actttcagaa gaggattgaa tacttatact tctgcccgaa
    20941 tcttggcagg tttgggtatc aaagcgattt tgattgaaga ataaaaaaac gaccgcggtg
    21001 ggtagccgcg gtctaggagt tatttttact tgaggagtaa aaatgaaaaa gtatataggg
    21061 ttgttggttg gtatgtatat agaatagaag ataaatgtga agaaattgtg cattatttct
    21121 tcacatttat taggttatag tttaagaaag tttaaacatt gcgcaattta attattttag
    21181 ctgtttcttt tcataaaaaa acatgttgtg actctgagac aaatcatagt ccatagtgat
    21241 gtctctagag taactacgcc taatattacg aggcatcata ttaatctatc agcactagta
    21301 cctaaactag catccctttc gaatattttt tcttatataa taaagtttgc tttcactcct
    21361 ctaatctcac caaatcgact ggcttgcgtt gtgtgatact aggaactgat tggtttagaa
    21421 cgacttttct attgtaatca tatttttcgc catcctcgtt gtcttctaat ccttcaatga
    21481 atgatcccta atgtgtgtat taaataattt aatttttttg ttcctattta tttaa
//
LOCUS       DASHRQ010000046        21088 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.046, whole genome shotgun sequence.
ACCESSION   DASHRQ010000046 DASHRQ010000000
VERSION     DASHRQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.046"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            476..727
                     /locus_tag="U2M88_001861"
     CDS             476..727
                     /locus_tag="U2M88_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374242.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            761..1039
                     /locus_tag="U2M88_001862"
     CDS             761..1039
                     /locus_tag="U2M88_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374243.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1636..1893
                     /locus_tag="U2M88_001863"
     CDS             1636..1893
                     /locus_tag="U2M88_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374244.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2006..2617
                     /locus_tag="U2M88_001864"
     CDS             2006..2617
                     /locus_tag="U2M88_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374245.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2796..3353
                     /locus_tag="U2M88_001865"
     CDS             2796..3353
                     /locus_tag="U2M88_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374246.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4052..4573)
                     /locus_tag="U2M88_001866"
     CDS             complement(4052..4573)
                     /locus_tag="U2M88_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEM8374247.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4772..8464
                     /gene="nifJ"
                     /locus_tag="U2M88_001867"
     CDS             4772..8464
                     /gene="nifJ"
                     /locus_tag="U2M88_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEM8374248.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            8653..9540
                     /locus_tag="U2M88_001868"
     CDS             8653..9540
                     /locus_tag="U2M88_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEM8374249.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            9537..10706
                     /locus_tag="U2M88_001869"
     CDS             9537..10706
                     /locus_tag="U2M88_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374250.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            10730..12085
                     /locus_tag="U2M88_001870"
     CDS             10730..12085
                     /locus_tag="U2M88_001870"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEM8374251.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(12157..13962)
                     /gene="glmS"
                     /locus_tag="U2M88_001871"
     CDS             complement(12157..13962)
                     /gene="glmS"
                     /locus_tag="U2M88_001871"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEM8374252.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            14371..14661
                     /locus_tag="U2M88_001872"
     CDS             14371..14661
                     /locus_tag="U2M88_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374253.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            14674..15699
                     /gene="sppA"
                     /locus_tag="U2M88_001873"
     CDS             14674..15699
                     /gene="sppA"
                     /locus_tag="U2M88_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEM8374254.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            15702..16361
                     /locus_tag="U2M88_001874"
     CDS             15702..16361
                     /locus_tag="U2M88_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEM8374255.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            16518..17450
                     /locus_tag="U2M88_001875"
     CDS             16518..17450
                     /locus_tag="U2M88_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374256.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            17604..18893
                     /locus_tag="U2M88_001876"
     CDS             17604..18893
                     /locus_tag="U2M88_001876"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEM8374257.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRLLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            19140..20390
                     /gene="clpX"
                     /locus_tag="U2M88_001877"
     CDS             19140..20390
                     /gene="clpX"
                     /locus_tag="U2M88_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEM8374258.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            20473..21060
                     /locus_tag="U2M88_001878"
     CDS             20473..21060
                     /locus_tag="U2M88_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEM8374259.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         7019 a         3601 c         4561 g         5907 t
ORIGIN      
        1 atacaaataa ttggtttatt ctggcaggcg taatgctttt ttctagtttc tatttattta
       61 gtaatgctct attagcacag cgagggaaat ataagtttaa aatattagat tagatattat
      121 ttttagaggg gataaatata aaatgaaaag aggttattta taatggaaaa agtattacca
      181 attaagagct actgtattcc aaggcttttc atcatagaat ttcaaaagta aagacatgtg
      241 tttcacaaat agtgtctatc ctggctaaat aattcttcca ctatctgctt taatttcagt
      301 agacttttcg gcaagttatt tgctaatgag atgaatagag attacaactc gcctataacc
      361 gcttaaaaca agctttttta aagtatagtt agcaagtcgt attcatatgt tcaaagagga
      421 gggcttatga actttgaatt cattccgtgt gaacagaaca gtagggggaa gtaaaatgaa
      481 agaaaagcct ataaataaat ggtcattgta taaaaattca atgtttggag ttgttatctt
      541 tggtgtttgt gctggattat tccttttcat attattacaa acggtgactc ttttctcttc
      601 taatcctact tatggatgga gagacagtat agccttaggg atgtttgcgg gaggatatgg
      661 attcttattg ttgttctcaa ttctcacact aagtccaatc catggactca ttcaactttg
      721 gaagtaagaa cgagccttaa acttttcgtt ctcggaagaa atggaaaaac acaacataac
      781 aggcagtatg tatgtggatg atcaatggtt tatcagcact gatgaggtaa aaatagctgc
      841 cattaggaga gattacctgc agaaaattca taaaataaaa gatcctaatg ggaaaaaggc
      901 aaggttaatt ataactttga taacatctaa tggtaagaag ttaaaacttg caggatcata
      961 tgataatgtt aaaaagttcg aacattggta tctgcaaaaa gacagcatta gtcttaataa
     1021 acaatttgaa aaaaattaat caaagtcatc ggcaacacga ataatttgac atcttgatat
     1081 tgtgatataa gcaaaaacgg catttctgag accgaacagc ataaaaaatt taaatgttgg
     1141 aatttatata agcaaaaccc aagaagctag atgcgataga gcatttaatt tcttggggtt
     1201 ttgcttggga atcagcatat caattaaaat gatcatctcg gtatttcaaa agtgggtacg
     1261 attacaaata gttggagcaa aatagtgggt aaaattacca ttccagtgtc tgtcttgcct
     1321 ctttttcgtc aatcacaaat agacgagatt cataggaaaa atcgatacag ggtatgattt
     1381 caatgatatt atgaccaatt acaaaataga ttgataaagc attttttgca tttttataat
     1441 agtttattga taataatgta tgagatacag tataatgatt taaaaggtaa cgtgttgtga
     1501 ccgaattaaa tgaattaatg cctatttttt atattcctta gtggacaaaa atggagacag
     1561 tgaagttagg agcattgtat cctttgttgc gtagttcaaa aaagtaagcg atttctataa
     1621 tggtcagggt aaacggtgag ggctgtccac gtaaatccta ctattgaaat cacaatgatc
     1681 ggacattcaa aaggtggtgc tgaagcagca aattcagtag ctataaatac gaatgcgatt
     1741 atatttaatc cagcgacagt aaatttatct gcttatgaat tagactcaaa cacatataca
     1801 gcaaacatgg tagcttatgt tgtcgaggga gatgcattaa atgttgtttt tggaggagta
     1861 tacacctatt ggagaattaa tcatgttaga tagacaatat gatgcacctt agtggagaat
     1921 gatatctcgc ataggtaact cagtaaaaaa tcaaggtatg gaagcaatta tctctgcatt
     1981 ggaagatcga tatagatagg cggatatgaa tagtaatagg gggaataaaa tggaatacaa
     2041 tggatcaatg agaattgtac aaattatatt ttggattaca tcgggaattg tgatcatagg
     2101 aggagttttt ttgatgttgc caagcttaat atttcctttc tttgcattaa tctcacctaa
     2161 aatacccgaa ccggaaatca cctatggaga atttccattt aggattgaat acgaactaga
     2221 tggacagatt aacataattg aagatactgt catagctgag tttaacgggt gtgagttcag
     2281 cgcaggttct atgaaaagag aacgaagatg gagaagtaga ttagctagtg gtagagaaga
     2341 tctacctttt ggggacgatc taggaatcta ttttagtcga ggaagtgcgc aatattatat
     2401 gggggaaaat gtacaatcaa tgtcattaaa accacatata gcccttagaa atctcgagga
     2461 aggttttaaa agagaggtag actttatatt aggagaaagt ggatacataa gaactgtact
     2521 taatttcgga gaagcgcaag aagttttgac acaatacgga attaccttaa tcaactggga
     2581 aatatctgaa ccgattgtta acaattttgg tgattagaaa acaattacga taaatagaaa
     2641 caagccaaat catcagcaag taaaagctga tgatttggcc tttcttatac gagtagtagg
     2701 aattcatagc gaatattatt acgaccagta gatattttga tattaaagga gtattacgaa
     2761 ctaatatgtg agaatgtaga ggtgatgtgc gaataatgag aaatttatca aatagaaaca
     2821 aaatattaat cattattgtt gttatagctg tgtttcatct gggaacgaat gcggttttaa
     2881 gtagaataat attgggtcct aagccaccaa gaccagaaat tacccgaggg gagtttgatt
     2941 ttagattaga atatgaagta gatggggaaa ggattgtgat agaagataca attgttgcat
     3001 tatttgatgg attttctgca gatgcaggta gcatggcatg gtatcgcacg tggcgtctac
     3061 atttagcaag tgatagacga tcacgaaata tcttattaga tgaattagag gatggtcgaa
     3121 gaatatatta tgtcccagaa agtgctaact attctatggg agatgtacag aaaaaaagag
     3181 agcctaatcc taattggtat ccatttaatg gcgtaacaat agaatatcca cgcaataaaa
     3241 cacctgaaat aggtgcaaaa tttataagtg gtttagaaga tctgtacgat aggtttggaa
     3301 tccgtcttat tagttgggaa cacgatccac caatagaaaa tcgctttgaa taatcgaaat
     3361 ctactaatcc atttcatcag ccagtgaatg ttgtaatgga acaatttggt ttagtgttca
     3421 aaaccattga aatactaggc tgtgatcagc aaaaatgaat ctgaataaaa aagagagcaa
     3481 ctattagctg agaggagtta atagttgtta gctttatacg aatcaaagtg gcaaatacag
     3541 atgcgtatgc atttgtgttt gccatcttgc gtttggtaga cgagataggt ataagctaaa
     3601 gaaacaactt gtttaaaata aacgacctag ctcaacaaat gaccatctaa tcaactaaca
     3661 gcatacataa aagcaactat ttttgaagta gaattgaaat cgggataact taaaaagtga
     3721 ttgggatact agtgagaatc accgtacata aatattactc gttaagggaa attgagaaat
     3781 tttgtatgaa aatttaagaa ttagttagta tgcgcaagtc aaaaatatga ataaattatt
     3841 tggtggagat aatttgctta gcttgtcata agaaatgaga taatctggca gatgcaagct
     3901 tttggacacc atttattcgg aataaggagg ctgggacaga agcgtttaac tccaagaaat
     3961 aagaaggagt tgcttctgct tccgccgttt atacgtgttt agagcgtgag acaaaagtgt
     4021 tctttacttt tgtcccacgc tccttatttt tttattttgt ttggatcaat tcttttaatt
     4081 cctctattga aatagattca tcaaagattt ctttacccaa gatgacagtt ggaacaaatc
     4141 gaatatgtgc ttgctcagct tcgttgatga cagcttggct cagctgttcg tctttttgtt
     4201 ctgtcagctt tagtttatct actgcaaaat ccgctacttc ttggagagaa agatgtttcc
     4261 actcgtcttg agtatcgaat atttgtttga tctctttgat cgctttttgg ccatcgctga
     4321 ttgtgagata ccggtgcatc acgttccctc gctgtaagct ttctttttct ttatcgaaca
     4381 atttgatcac tcgctgcaat tgcccgcttt ggactgcttc ttctaatagc tcataagatt
     4441 cttcaaacca ttgtcggcaa tacggacaag ctaagttgat gaattcgacc atttttttag
     4501 gagcattcga tgaaccataa aggattcctt tttgtgtatt tgttttcgtt gcatcaatca
     4561 ctgatatatc catgttaaaa cacctctttc gcttatagtt tatacatgtc tgacaaaaaa
     4621 aggcaacgat ttagactgtt tgtctaaaaa ttagatgacc aattcaaata gatacatgta
     4681 aaatattttt ataaataaaa gaaaaaaatg gcatgtttta cgttataatg gaacagataa
     4741 taagagagaa tatgaacaag gatgatgaat catgcgaaaa atgaaaacaa tggatggcaa
     4801 tacggcagcc gcatatattt cttatgcatt taccgagatt gctgcaatat atccaataac
     4861 acccagttcg accatggctg aattagtaga tcaatggtca gcagcaggaa aagaaaacgt
     4921 attcggtcaa ccagtaaaag tagtagaaat gcaatccgaa gcaggagcag ctggtgtggt
     4981 ccatggatca ttgaaaactg gtgcactgac aactacatat accgcttcac aagggctgct
     5041 tttgatgata ccgaatatgt acaagattgc tggggaacta ctgccaagtg ttttccacgt
     5101 tgctagtcgg gcagtgacga caaatgcgtt gaatattttc ggagatcacg gagacgtgat
     5161 ggctgcaaga caaacaggat ttgctatgtt agctgaaagc agtgtacaag aagtgatgga
     5221 tttagcacct gttgcccatc ttgcttctat tgaaggagaa gtcccttttt tgaatttctt
     5281 tgatggattc cgtacgagtc acgagattca aaaaatcgaa gttttggaat atgaagaatt
     5341 agctcctctg atcaatcagg agaaattagc ttcatttcgt aaacgaggga tgaatcccaa
     5401 tcatccgact gttagtggta cgaaccagaa tccagacatc cattttcaac aacgggaaac
     5461 gatcaacccc tattatgata aattacctga aatcgttcaa aaatatatga aagagatcaa
     5521 ccgattacga ggaacttctt atgacttggt gacttattac ggagcagacg atgcggaaga
     5581 agtcattgtt tctatgggat cagttgcaca gacgattgaa caaacgattg attacttgaa
     5641 tgcaaacgga cggaaaacag gttttttgaa tatccatctt tatcgtcctt ttccagtgga
     5701 aacattcgtg caaaagttgc ctcatacagt aaaaacaatt gctgttcttg atcgaagtaa
     5761 agaaccaggg gcaggtggag aacctctctt attagacgtt caaagtgcgt tatatgattc
     5821 tgaacttcgc ccggctgtta ttggtggtcg ctacggattg gggtctaaag atgtgactcc
     5881 cgaccaaatc tcagcagttt ttgatgaatt gaaaaaagac ccaagcatac gcaaaaaacg
     5941 gtttaccata gggatcgtgg atgatgtgac ttatcaatct ttggaaccaa aggaaagtct
     6001 ggatttgaca gaaccgcaaa cattccaggc caagttttgg ggatttggat ctgacggaac
     6061 agttggagca aataaatcag ctatcaagat tattggagat cacaccgata aatatgcgca
     6121 aggctatttt tattatgatt cgaaaaaatc tggcggtctg actgtctctc atcttcgttt
     6181 tggagatacg ccgatacgtt cagcgtatct tgtcgagcat gcggaccttg ttgcctgtca
     6241 cacaccagct tacctgcata gctatgactt ggtcaaagga ttgaagccag gtggcatttt
     6301 tttattgaat actctttgga gcgacgaaca attagaaacg catctgccct taaaattaaa
     6361 acgttacttg gctgaaaata acattagatt ctatacgatc aatgccatga gattggccca
     6421 agaagtcggt ttgggacgcc ggatcaatac ggcaatggaa acggcttttt ttaaattagc
     6481 tgacatcatt ccttttgatg aggtgcttcc gctgctaaaa gaagaagccc tgaaaagcta
     6541 tggacataaa tcaatggcta tcgtagaaaa aaacatacaa gcaatcgatc ggacgaccga
     6601 actgcttcat caagtcgcga tcccagacca ttggagaacg ttggagatgc ctgaaaaaat
     6661 caataaaagg aacactggtt atatacaaga aatacttgaa ccagtgaatg cacaggaagg
     6721 aaatcaactc tctgttggta cacttgtagc aaatggaatg acaactggcg aaatgccttt
     6781 aggaacggct tctatggaaa agcggggcgt cgctttagaa gtacctgagt ggattagtga
     6841 tcgctgtacg atgtgtaatg aatgtgcctt tgtttgtcct catgctgcca tccgtccatt
     6901 tttagcagat gaagaggaaa tggaagaagc gccagaagga tttatcgtga gagaaatgcg
     6961 aggcgcagac ggcgtaaaat accgtatcca agtttcagtg gaagactgta caggttgtgg
     7021 tctgtgtgta gaggcttgtc ccgcaaaaga aaaagcttta gtgatgaagc cttatgaaga
     7081 acagaaagaa caagctgtga attgggcttt tgcaatgaca ttaagacaaa aagcaaatcc
     7141 agtcaaagga aagaatactg tcttgtctac acaattcaat caaccgcttc ttgaattctc
     7201 gggcgcttgc tcaggctgtg gcgaaacacc ttatgtaaaa ttattgacac aaatgttcgg
     7261 tgatcgaatg atgatcgcga atgcgacagg ctgttcttct atttggggcg gtgcttctcc
     7321 tgcttctcct tatacgacca atgaatgtgg tcaagggcct gcatggtcaa attctttatt
     7381 ggaagataat gctgaatttg gttatggtat gcttctagcg gcacagactc gtcgcgaggc
     7441 tttggcaagt tcaatcaaag aagtattgcc aaaagtatca gaagaactac gagaattgat
     7501 ggaagactgg ttagtacaca tgtttgagag tgaaggaacg cagcaaagag cagcaaaact
     7561 gcaagctgcg atcgaaagcg aacgatcaat tttccctgaa ctggaggata tatacaagag
     7621 aaaggatcta ttcgtaaaaa acagccaatg gatgattggt ggagacggat gggcttacga
     7681 cattggtttt ggcgggatcg atcatgtact tgccagcgga gcagacgtga atatccttgt
     7741 tttggataac gaagtctatt ccaatactgg cggtcaaacc tcaaaagcaa caccagcttc
     7801 ggcaatcgct aaattttcgg ctagtggaaa gtatgcttca aagaaagatc taggaatgat
     7861 ggcgatgaca tatggaaatg tttatgtggc gcaaattgct tcaggtgcca atcaaatgca
     7921 aacgatcaag gcatttgaag aagcagaaaa gtatcctggt ccttctctga ttattgccta
     7981 tacgccttgt atcacacatg gcttgcttgg cggaatgaaa gaatcgctta aagaagcaaa
     8041 agaagctgtt tcttctggtt attggtcatt atatcgatat aaccctacaa tggtcgaaaa
     8101 aaacaagaac ccaatgactt tggactacaa aaaacctgat ttctctgcta tgcctgattt
     8161 catgaggaaa caagttcgct tttcctcgct agataacgca aatccaaatc ttgcaaaagc
     8221 tttatttgaa aagactgtcc acgacgctaa aacacgtttt tacaactatg ctgcactgac
     8281 gggagaatta gaaaaaatca agaaacgact tgaaccagag gaatctatcg atgtacccgt
     8341 taagactaga aaagaaaaaa cgacggacct tgaagcagaa gaaagaagag ctgctagaag
     8401 agctgccaga gcagaacgac gtaaaaaaat ggaacaagct acaaaagaaa aaaaggaaaa
     8461 ataacagaaa aaatataatg aataattctc tgaagtttaa aaatatttat tgcagagaaa
     8521 aataaaacgc atacttgcaa ttttcttgaa aattatgcgt ttttctcaag aaaaatacga
     8581 gcatatcagc ctctaaaatt aaaacatatg atatactaga gaggaaatct caaaagagaa
     8641 gagaggcttt ttatgtcatt tcaaatcggg gaacttttta atttggacta ttggcagcaa
     8701 atgttttccg gtgacttgtt ctctctgaat tttttggtga acatacttga tattctggtt
     8761 gtctggtatc ttgtatacaa actgatccaa ctgctgcgag gcacaaaagc gattcagctt
     8821 ctgaaaggtg tagctatttt tattgttatc cgaatcgtag cggaaatcat tggtctgcac
     8881 accttgtctt ggctgatgaa ccaagtcatt acgtacgggg tgatagctgc tatcgtgatt
     8941 tttcagccgg aaatccggag aggactggaa catcttggac gcagttcgct tttcaaacaa
     9001 tcaaaaagcg aacagcagga agatgagaag atgatcttgt cttttgacaa agccatccaa
     9061 tatatgtcca agcgaaaaat cggtgcgttg atcactatcg aacgtcatac tggattagat
     9121 gagtatattg agacagggat tgctttagat gcagatatca ctggggagct gctgatcaat
     9181 atctttattc ctaatacacc gctgcatgac ggtgcagtta ttgtaaaaga aggaaagatc
     9241 gcagtagcaa gcgcctatct tcctctatca gaaagtatgt tgatccctaa agaattcggg
     9301 actcgacacc gggcagctgt cggtataagc gaagtcagtg acgcgattac catcgttgtc
     9361 tctgaagaaa ccggggacgt cagcatcacg ttagataatg aactgatggc tggcttatcc
     9421 cagcaagaat atttggcaat cttgcgtaga gaactaattg ctgaagagaa aacaaaaaac
     9481 aagaaaaacg tgattcaatc tttcctagac ggtatgacta agggagggga tcgcaaatga
     9541 tctcaaaaga gaaacggaca agcattcttt acagttttat tgcattgctg ttcagtattg
     9601 cgctgtactt caatgcaaat ggccagtccg ttccaaatac gctttctgga aatgaagcgt
     9661 ataatcaaac agttacgggg gtgccgatca agatttctta tgattcaaag cgctactata
     9721 tccatggata tgaaaatgca gtgactgtca aactttccag tgcaaaccga gttcagttga
     9781 atgcagaggc aaacgaagat acacggatgt tccgcgtcac agcagatatc actaagctgg
     9841 gcgaaggaac acatgaagtt ccgctaaaaa ttcaaaattt gagcagtgct gtcacagcaa
     9901 agatcgaacc atcgactatt acagtaacgg ttgagaaaaa ggtaataaaa gatttcgatg
     9961 tggaaacgtt gattccttca acgttcacac catctggata tgaacttgat gatacttccg
    10021 ttagtcctaa aaaagtctct atcactacag gtgacaaaac attggcagaa atcaaacgag
    10081 tagtagctga tgtggacgct tcgatggtca cagatgatgg gattaatagt gaggtcccaa
    10141 tacaggcgct gaatgcagca ggggaagtat taagtatcgt ctctgatcca gtgcaagtaa
    10201 cagtgagagc tgattcgatc aagccaagca agtcggtacg actttatggg atacagcaag
    10261 gaacaccagc tgccggggta gaaagctatg atttcagttt ttcaaaactg gaagcagaag
    10321 taagcggctc tagcgatttg cttgcatcga tcggtgacag tattgctgta ccgatcaatg
    10381 tctcaggtat caatcatcga acaactcgta aaattgaaat accagtagaa gaggggttaa
    10441 gtatcaagcc cgaatcagtt agtgtggaga ttacacccgt gttgcaaacg tcaagcagta
    10501 gctctgcttc gacgagcagt cggacacaag aaaatacaag tacgagtaga agtatttcat
    10561 ctgctgattc aggaacggaa ccatcgacaa caaccatttc ttccagttcg gaatctggga
    10621 caagcagctc gaccacaagt acagaagaaa catcagaaac aagcgaatca acacaaatga
    10681 gcagaacaaa ttcctcagaa aattaagtta aaggagtaat tttacaacta tgggtaaata
    10741 ttttggaaca gacggcgtac gcggtgaagc caacaaggaa cttacgccag aacttgcatt
    10801 taagctggga cggtgcggag gttatgtcct aagccagcat gaagaagaag gaaaacgccc
    10861 acgcgtgtta gtagggcggg atacaagaat ctcaggtcaa atgttagaag aagcgctgat
    10921 ttcaggactt ttatcggtgg gtatcgaagt gttccagcta ggtgtgatct ctacaccagg
    10981 tgttgcttat ctgacacgtt tgcaaaaagc aagcgcaggg gtcatgattt ctgcttctca
    11041 taatccagca caagataatg gaatcaaatt tttcggtgca gatggtttta aactagtaga
    11101 tgaccaagag ctagaaattg aagcattatt agatgctgaa gtggatgaat tgcctcgtcc
    11161 atcagctgaa ggattgggaa ctgtagaaga atttccagaa ggcttattga aatattctca
    11221 attccttgta caaacgatca acggtgacct ttccggtttg accatttgtg tcgatgcagc
    11281 aaacggagca acttcaacaa gtgttaatcg tttgtttgcc gatcttgaaa cagattttta
    11341 tacaatgggc acaaatccag acgggttaaa tatcaatgac ggcgtaggct ctacccatcc
    11401 agaacgttta gctgagatgg tcgttgaaaa aggagcagat gctggactag ctttcgatgg
    11461 cgatggcgat cgtattattg cggtggatga acttggaaac attgtcgacg gtgataaaat
    11521 catgtttatc tgtgctaaat acttggcaga aaaaaatcga ttgaaaaaag atacgatcgt
    11581 tacgactgtc atgagtaact taggattcca caaagcggtg gaaggaatcg gtttgaaaga
    11641 tgtcgttaca caagttggtg atcgttacgt tgtagaagaa atgcgcaaaa acgattataa
    11701 ctttggcggc gagcagtctg ggcacatgat tttcttagat tttaacacaa ctggagatgg
    11761 gatgctttca ggaattcagt tactgaatat catgaaacag acaggtaaga aattatcaga
    11821 gcttgcagca gaggtaacaa tctatccgca aaaattagtg aatatccgcg taagtgataa
    11881 aaacggtgcg atggatgttc cggcaatcaa aaaagtgatt gaagaggcag aagctgaaat
    11941 gggatcagaa ggaagaatcc tagttcgtcc ttccggtaca gaaccattac ttcgagtgat
    12001 ggcagaagcg cctactgatg agaaagttaa ttactatgtc gataaaatcg cggcagtagt
    12061 caaagaagaa atcggactgg attaagagat attttcataa aaaataaaag aacaaggacc
    12121 agtgtcagat gtctgacact ggtccttgtt cttttattat tctactgtca ctgatttcgc
    12181 taaattacgt ggtttgtcaa cgtcgaatcc ccgtaatagc gttgcgtaat atgcaagtaa
    12241 ttgagtcggt acgactgtgg ttagagtagt tagataaggg tggacagttg gtagcaccag
    12301 ctggtcactt tccttttcta atccctctga gacaataact agtgttttag cgccacggct
    12361 ttcgacttct ttcaggtttc ctcgagtatg cgcaccagtc acttcttcac tgatgattcc
    12421 gatcactggt gttccttctt cgatcaaagc aatcgttcca tgtttcaatt cgccagcggc
    12481 aaaaccttct gcctggatat aactgatttc tttcaatttc aaagcagctt caagagaaac
    12541 gttgtagtca gcaccacgtc caatgtaaaa ggcatttctt gtatcgctga gatatacttc
    12601 tgccaactct tctaaataat ctttttgggc gatgaccgat tccatcgcat tagcaactaa
    12661 actcaattca tgagtgatat cgaatacaag agaagtttca aattcttttt tatcaccgat
    12721 agctttggct aataaagtta gtacagctat ttgtgcagta taagctttcg ttgatgctac
    12781 tgcgatttcg ggtccagcat gcaatagcag tgtaaaggat gcttcccgcg aaagagtaga
    12841 accagcaaca tttgtaatcg taagtgaagg gtagccgaga cggttgattt ttaccagcac
    12901 ttggcggctg tctgctgttt cgccactttg actcaagaaa ataaagaatg gtttttttga
    12961 aagaagaggt gtgttatagc caaattcact ggctaaatga acctcaacag gaatctgtgt
    13021 caatttttcc aaagtctgtt tacctgctaa ccccgcatgg taactagttc cgcaagcaac
    13081 gatatagatt cgatcgctat cagacaatgt atcgaccagc ttttgatcaa gagagacacg
    13141 attgtcttct gttacgtatt tttgagcgat acgacgcatg ataataggct gttcgtcgat
    13201 ttctttcagc atataaaaag gataggtacc tttctcgata tctgaagcat ctacttgtgc
    13261 ttcgaatgaa gaacgttcta cgatgtcacc tgcaaaattt tgaataataa ctttttctga
    13321 tgtgacggta accatctctt catcttcaat ctcaacgaat tccttcgtat gagcaagcat
    13381 agccattgca tcgctggcaa tcacattgaa tccatcgccg agtccgatta atagtggact
    13441 tttattttta gcgacgtaaa taacatcagg tgctgtccga tcaatcaaag cgaaagcgta
    13501 agaacctttg atgacccgca aagcttttaa aaacgcctct tgcgttgtag tatccttcgc
    13561 gaaagtttca attagatgag cgataatctc agtatcggtc tcaccaataa agtggtcatt
    13621 tgctaaataa gcaattttaa gttcttcaaa gttttcgatt acgccgttgt gaacgagcac
    13681 gaagcgtcca ctttgggaag tatgtggatg ggcgttttct tcagaaggtt gcccgtgagt
    13741 tgcccatcgt gtatgaccaa tacctatatt tcctgtagta tcaatattta ttttgttttt
    13801 caaattttga attctgcctt gtgctttgac cagataatca gttgtgccat ctgagacaaa
    13861 gataccagct gaatcataac cacggtattc taatttttct agaccgtcaa tcaatcctgg
    13921 tgttacattt tttaatccaa tcattccaac aattccgcac ataaaaaaca tcctactttc
    13981 tataatgaat tcgtatgaat gcttccagct gcaggaaaat ccttttgttt tatctttgca
    14041 gacatttttt gtggattttc tgtagagtgc agtccctagg gccatccgcc gaatctttcg
    14101 ataaaccctc tcctcgtcat ctgtttacac agacctggcg ctatcctaaa aggtataact
    14161 atcctccttt ttgtttttta tctggaaaca tttaaaatca taaccttttg cgaatgttct
    14221 tgtcaacaaa aaaagaataa aaaataaatt ggtatatatc aaattctttg tcaaatgtcc
    14281 tttttatgtc aaaaaataat gggtttttat ctatcatctt tttcaggagt tcggtacaat
    14341 agaaagtaat cggatagagg aggagtaaaa atggattcaa aaattgagat catgacactg
    14401 ggaatgttaa aaaaacaatt gtccaaattc gaagcatcag ctggtgtatc agatgatacg
    14461 aaaatatttt tagatactgg ctgggatagc atccaagaaa tcgcacctga tgccttggaa
    14521 gttgctcaag ctcgtgagtt tacggtagaa gatgaattaa caaaagagtc gttttccggg
    14581 tatgcgcggg aagaaaaagc agagcggttc gacacttctg agcagtcaga aactgtgatc
    14641 gtcataaaaa acttgtatta gaaaggagaa agaatgaata aaagacgttg ggcagcagtt
    14701 ctgattgctg caggattatt tgtggtttcg ttagtttcag ctagtttgac taagcctcag
    14761 gaaaacgatc aaattgaggg attgaacagt tggttatatg gggatgaaga actagctcca
    14821 aatgtgttgg aagagggaga cagtacgcaa aaaatcgtaa aattatcagt agatggaacg
    14881 attgctgcgg gagggtcttc aggtttattt gcttctgaag gttacaatca taaaaatttt
    14941 atggaacaac tgaaggcgat tgaagaagat ccaacagtca aaggtatctt tttagaagta
    15001 aattctcctg gtggcggtgt ctacgaaagt gcggaaatcg ctaagaaatt ggatacgatt
    15061 cgtaaagaac atgatatccc aatgtatgtc agcatgaaaa acatggcagc tagcggtggc
    15121 tactatattt ctgctcaagc agataaaatc tttgcaacag aagaaacagt gaccggctca
    15181 attggtgtga tcatgtcagg attgaattat tcaggacttc tagaaaaact gggtgtggaa
    15241 gatacaacag ttaaaagcgg agcgttgaaa gatatgggtt caggaacgcg accagagaca
    15301 aaagaagaag aagcggtgct tcaagcatat atcgacagcg catatagtcg ttttgtagca
    15361 atcgtcagcg agggacggaa taaatctgaa gaagaagtca aaaaaattgc tgatggaagg
    15421 atttatgatg gtgtccaagc aaaagaagta ggtttagtcg atgaattggg atttcctgaa
    15481 gatgctttaa aagctatacg aaaggaacaa cagcttgaag atgcggaact agtagaatat
    15541 tcttccacgc catctgggtt tgctagtaca tggttaggaa gtaaactagc agaattccag
    15601 ggattgaagc cttctgagac tagtcagatc ttatccgttc ttgagaatct agggacagcg
    15661 gaatcaccaa aagcaatgta ttattacgga ggtgagtaag catgtcagag ctttcaaatg
    15721 aaaataagga acaagaaaaa gataagcaac agcagcctgc tgtaggacct gactccaact
    15781 tttcccaaaa ggtactgagc tcttttgaaa gagagccttt gacgcccgaa gaaatcaaaa
    15841 agaaacaacg taaatggaaa aagtatacgg acacaagaaa tgaaccgcca aaagcaaatg
    15901 attttcctaa gtatttctat gcaggttttt ttatccgaat gtttgccttc ttggtcgatc
    15961 ttttgtgtat cggagctatt acgcgtttaa cacttggtat tgccaaaaat ctaggttggc
    16021 ttaatttttc agattcttat ctgagtctct acggattcac agccttactt atttatctag
    16081 cgtattttat tttattgacg aaattcaatc atggccaaac gattgggaaa atgatctttg
    16141 gtattcgagt catcagcttt gatgaagaag aacttagctg gtcaaccgtt ttagttagag
    16201 aaggtgcttg ccggttcatt ctaaaatttc ctttgctgat gttagggtat cttccagcag
    16261 tctttaatca gaaaaagcaa catctgggcg attttttcac taatacaagt gttgttacga
    16321 tcaatctgat caaagcgttc aaccaggaag tcaacagctg atgagcaata aaaagcagta
    16381 acagttgaaa gcaccttcta aaagtcagat ctaaaatcta gcttttagag ggtgcttcag
    16441 ttatttttaa ttgtcatgct ttttttgttt tgctactatt gaacatgagc tgaatatgag
    16501 caagaggagg acactgcgtg aagccgattg attttccaga aaaatatgag aatttaatgc
    16561 gagtggcaca acaagcacta gcaaaccagc aatatcagca ggcaaaagaa ttatttcaac
    16621 gagcttacga gttgaaagaa agctttgagg ctaatagcct gcttgttttt tgtctttacg
    16681 agttggacga gaaaaaagaa gcattgaagc aagcactctt gcatgaaaaa cagtatcttc
    16741 aaaatgagga gtttgctgaa ttttattttg acttgttaat cagtacacaa gattttttat
    16801 atgctagaaa attgatagca agtacagatt tttatgaagc tttcgagcag cgggtcattg
    16861 aaaaaatcca atttgcagaa gaattatccg gacaaatgga gaggcagaag atcaaggcac
    16921 tccacaataa atcggaggaa cttccttcgt tggaacctgc acgtcagctt gcgtctattg
    16981 atgagatcga acagcttcct tatcatgagt ttatccaaac agcgagtaaa cttattgtat
    17041 cgccagaggt ccatatttta gcaagggcaa aattactgga gacacttagg caattgaacg
    17101 aatgccatcc tgttttctat ttgacgattg aagaaaagtt agtcaaagtc ataccaaaag
    17161 atctgccaaa gccgcagcag caggcaagtt accagcaatt atgtgtattt tcagatcgtt
    17221 acgggaatga agatgcgctg cttagttccg tgttaaaaga agaattcacg ttgcaatctg
    17281 caattgttta tcctgtctat gatacataca tcgaagaccc gaagcgctgg tttcaactga
    17341 ctgtagaggc gtgtacagga aaaacgatgt atgaaggtac agaagatgaa aaacaagatt
    17401 ttttaaaaaa aagagaaaaa attttgcagc aaatgatctt cttccattag attttatcag
    17461 tgaaaaagat aaaaatgtta tgaaaaacaa ggattctatc cattcaatat gtttacaatt
    17521 tgaaagggtt agtgtactat ttaaaggtat gcatgaaacg tttttgatgt ataaaatgaa
    17581 tttctaaact cggagggaat taaatgactg caacatttga aaaaaaaggc acgaatgatg
    17641 gtgttttaaa atttacgatt gcgcaagacg aaatccaaaa aggattaaca actgcgttta
    17701 ataaagtgaa aaaaagcttg aacgtacctg gattccgtaa agggaaagtt tctcgtcaag
    17761 tattcaaccg tatgtacggt gaagaagctt tatatgaaga tgcattgaat gctgtattac
    17821 cagaagctta tgaagctgct gtcaaagaag ctggcatcga tcctgtttct caaccaaaaa
    17881 tcgatgttga aagcatggaa aaaggtcaag actgggtaat cactgctgaa gtaacagtga
    17941 aaccagaagt aaaattagga caatataaag atctagttgt tgaaaaacaa gaccgtgaag
    18001 taacagatgc tgacgtggat gcacgtatca aacgtgaaca agaagcacaa gctgaattag
    18061 ttatcaaaga agatgctgca gctgaaaatg gcgatacagt agtaatcgac ttcgaaggct
    18121 ttgtagatgg cgaagcgttt gatggcggaa aaggcgaaaa ttactcactt gaattaggtt
    18181 caggctcatt cattccaggt tttgaagatc aattagttgg tcataaatca ggtgaaagtc
    18241 tagatgtaaa tgtaactttc ccagaagatt atcaagcaga agatcttgct ggaaaagaag
    18301 cagtcttcaa agtgacaatc catgaagtaa aagcaaaaga agtgcctgaa ttagatgacg
    18361 aattcgcaaa agatgttgat gacgaagtat caacattaga cgaattgaaa gaaaaatatc
    18421 gcaaagaatt agtagaaaca aaagaaaaag cagcggacga tgcaaaagat gaagcagcga
    18481 tccgtatggc tgttgaaaac gcagaaatcg ttgaattgcc acacgtgatg gttcatgatg
    18541 aagtacatcg ttcaatggac gaattcttga acaacatgca acgtcaaggt atctctcctg
    18601 aaatgtacta tcaattaact ggttctacag aagaagactt gcataaacaa ttcgaaggtg
    18661 aagcagaaat gcgcacaaga acaaatcttg tgatcgaagc aattgcagct gctgaaaatc
    18721 ttcaagcaac agaagaagaa atcaatgcag aaatccaaga attatcagaa acatacaaca
    18781 tgccaatcga acaaatcaaa cgcttgttga cagaagatat gctggaacat gatgtgacaa
    18841 tgaaaaaagc aatcgaattg atcacaggta cagcgacaga agcgaaagaa tagaaatcaa
    18901 gtaataaatc atttctgaag aagctacttc tagaaaattg tttattcgaa ataagggact
    18961 gtgacacgat tttgtcacag ttccttattt tttgttaaga gtaaaaaatg caaaggtata
    19021 ggtcagttga tcctttgtta aacacttttt ctttttacta aactatgtta tgatagtaaa
    19081 agactgtaaa gtctttgata gtttcttgca tactttttgc aagttaaggg gtgaagacca
    19141 tgtatgacaa tccaagtagc aatgagaccg tacgctgttc tttttgcggg aaaacccaag
    19201 aagaagtgaa aaagatcgta gctggtccag gggtctacat ctgtaatgaa tgtatcgacc
    19261 tatgtaaaga aatcattgat gaagaatttt atgaagaagc tgtacgcgaa ttcactgaag
    19321 tccctaaacc actagaaatt cttgaagtat tgaataacta cgtgattgga caagatcgcg
    19381 caaaacgctc gttagctgtt gccgtataca atcactataa acgtgtcaat cagcaagcac
    19441 aagaaaacac agatgatgtt gaactgcaaa aaagcaatat ctgcttgatc ggacctacag
    19501 ggtctggtaa aacattttta gcacaaacat tagcaaaaac attgaatgta ccttttgcaa
    19561 tcgcagatgc gacaagtttg acagaagctg gttatgtggg ggaagatgta gaaaacattt
    19621 tgcttaaatt gcttcaatct gctgattaca atgtggaact tgcagaaaaa gggatcatct
    19681 atattgatga gatcgataaa attgcgcgta aaagcgaaaa tgtatctatt actcgtgacg
    19741 tttccggcga aggtgtccaa caagccttgc tgaaaattct agaaggaact gttgcaagtg
    19801 tgccacctca gggcggacgt aaacatccac atcaagaatt tattcagatt gatacgacga
    19861 acatattgtt catcgtaggt ggtgcatttg atggcatcga aacgattgtg aagaatcgta
    19921 tgggcgaaaa gacgattgga tttggtacac aaaaccaaaa actttctgaa gacgaaagtg
    19981 tcatgcagca tatcattcct gaagatttac taaaattcgg attgatccct gagtttatcg
    20041 gacgattacc tgttatggca gctttagaga aattgacaac agatgatctg gtacggatct
    20101 tgactgagcc gaaaaatgcg ctagtcaaac aatatcaaaa acttctttct ctagataata
    20161 ccgaacttga attcgaacct gaagcgttga gagccattgc gaaaaaagca atcgaacgca
    20221 ataccggtgc tcgaggactt cgttccatca ttgaagaaat catgatggat gtgatgtttg
    20281 atatcccttc aaatgaaaac atcgaaaaag tgattatcac aaaagaagca gctgaattgt
    20341 ctggtgagcc aacagtaatc tataaagatt cagagaaaaa agcgggttga tgaaaagagg
    20401 ctgccgtaaa agtcattttg gcttttgcga cagccgcttg ctttattcat caccttgtta
    20461 aggagagaga ccatgaaagt ccatcaagct gaaattatca taagtgcagt ggcaccaaaa
    20521 caatatccag atacacaatt gccagaaata gctctggcag gacgatcaaa tgtagggaaa
    20581 tcttctttta tcaacacatt gattgatcga aagaatttag cccgtacttc cggtaagcca
    20641 ggaaagaccc agacattgaa tttttatttg atcgagaacg cattacattt tgtcgatgtt
    20701 ccgggatatg ggtatgcaaa agtatccaaa actgagcgag caaaatgggg aaagatgatc
    20761 gagacatatt taacgactag agaccagcta cgtgcggtcg tttctttagt agatttgcgt
    20821 cataagcctt ctcaagaaga tatccagatg tatcaatttt tgaagtatta tgatatccct
    20881 gtaattgttg ttgccactaa ggcagataag atccctcgtg gtaaatggaa caagcatgag
    20941 agcgaaatca aaaaagcgtt ggattttgat cgcaatgaca cgtttattct cttttcttcc
    21001 gttacaaaac agggaaaaga agaagcgtgg gcagcaatcg aacaagcaat tgaatcataa
    21061 aaagaataaa aaagacttac cagtcaaa
//
LOCUS       DASHRQ010000047        20956 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.050, whole genome shotgun sequence.
ACCESSION   DASHRQ010000047 DASHRQ010000000
VERSION     DASHRQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.050"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..144
                     /locus_tag="U2M88_001879"
     CDS             <1..144
                     /locus_tag="U2M88_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM8374260.1"
                     /translation="DFREVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYS
                     SLI"
     gene            complement(1010..1540)
                     /locus_tag="U2M88_001880"
     CDS             complement(1010..1540)
                     /locus_tag="U2M88_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HEM8374261.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1537..2154)
                     /locus_tag="U2M88_001881"
     CDS             complement(1537..2154)
                     /locus_tag="U2M88_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEM8374262.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            complement(2760..2957)
                     /locus_tag="U2M88_001882"
     CDS             complement(2760..2957)
                     /locus_tag="U2M88_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HEM8374263.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYK"
     gene            complement(3135..4667)
                     /locus_tag="U2M88_001883"
     CDS             complement(3135..4667)
                     /locus_tag="U2M88_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEM8374264.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            complement(4803..5888)
                     /locus_tag="U2M88_001884"
     CDS             complement(4803..5888)
                     /locus_tag="U2M88_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEM8374265.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            complement(5903..6622)
                     /locus_tag="U2M88_001885"
     CDS             complement(5903..6622)
                     /locus_tag="U2M88_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEM8374266.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            complement(6619..7929)
                     /locus_tag="U2M88_001886"
     CDS             complement(6619..7929)
                     /locus_tag="U2M88_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEM8374267.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            complement(7956..8546)
                     /locus_tag="U2M88_001887"
     CDS             complement(7956..8546)
                     /locus_tag="U2M88_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEM8374268.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(8864..9925)
                     /locus_tag="U2M88_001888"
     CDS             complement(8864..9925)
                     /locus_tag="U2M88_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM8374269.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(10493..11560)
                     /locus_tag="U2M88_001889"
     CDS             complement(10493..11560)
                     /locus_tag="U2M88_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEM8374270.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(11580..12059)
                     /locus_tag="U2M88_001890"
     CDS             complement(11580..12059)
                     /locus_tag="U2M88_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HEM8374271.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(12076..12537)
                     /locus_tag="U2M88_001891"
     CDS             complement(12076..12537)
                     /locus_tag="U2M88_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEM8374272.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(12551..13801)
                     /locus_tag="U2M88_001892"
     CDS             complement(12551..13801)
                     /locus_tag="U2M88_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEM8374273.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(14179..15630)
                     /locus_tag="U2M88_001893"
     CDS             complement(14179..15630)
                     /locus_tag="U2M88_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEM8374274.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(15951..16715)
                     /locus_tag="U2M88_001894"
     CDS             complement(15951..16715)
                     /locus_tag="U2M88_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEM8374275.1"
                     /translation="MIDLHCHILPGIDDGAESLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(16743..17441)
                     /locus_tag="U2M88_001895"
     CDS             complement(16743..17441)
                     /locus_tag="U2M88_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEM8374276.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(17453..18232)
                     /locus_tag="U2M88_001896"
     CDS             complement(17453..18232)
                     /locus_tag="U2M88_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEM8374277.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(18248..19192)
                     /locus_tag="U2M88_001897"
     CDS             complement(18248..19192)
                     /locus_tag="U2M88_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEM8374278.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(19238..20017)
                     /locus_tag="U2M88_001898"
     CDS             complement(19238..20017)
                     /locus_tag="U2M88_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374279.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            20444..>20956
                     /locus_tag="U2M88_001899"
     CDS             20444..>20956
                     /locus_tag="U2M88_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEM8374280.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFP"
BASE COUNT         6693 a         4150 c         2714 g         7399 t
ORIGIN      
        1 gattttagag aagtgaatca gacatttatt tccagtgtca gcaatcaacg taatcatatt
       61 ccaagaaaat cattgaatta cagaacacca atcgaaatat ttttgagcta tgtacaagaa
      121 gcattttatt ctagcttaat ttgacaaatc atataattta aaattaacat tgattctcct
      181 atgaaaatat cttttacctt tctcctgtga aaaggaaatt tcctttttta tagtgttatt
      241 gatataatca gaatattcat caaactctgt atctggagtt aaaaatctac agtaacatct
      301 ataagctttg ctagtaatct tcttgggttc tctctccatt gtcaagcaac atcaaaacac
      361 ctacttacta ttcgattctt ttctgccaaa aacgagtcag catcactcac aacttatgtg
      421 ttgctgactc cgactagtcc tttgttcaat atcaacttat taaacagata taatattttt
      481 ctttatttcg tatactccaa gacttccatc tttctttcat cttatctctg aaaaaaacac
      541 agcaaagtgc ccttaataaa tatacattag aatttgcaaa ttttttcatc ttaagatata
      601 tcctattcat attctttaca atcctaatgg gtgattacaa atctatttag ttcaagttct
      661 acattataaa cacctttctt gatagtatca tctgctgcac aagcgatgtc gatccactta
      721 ttaggggatt tacgtgctca aaagtggcta ttccttttgg gaagtccatc cataataatg
      781 gtccatggat ttttttaggg tcttgtcgtt caaatttctc aaaaacttta ttatacttcc
      841 atagttttat aattatttgg tttcactttg gctcgttcaa acaccaactt gtatttcttt
      901 tcaacgttcg aactggagcc ccccctataa tctcattatt attttcatga ctttttacta
      961 ccgtggaatt acaagcaaca acaatattat ctgctaaatg aacacctttc tatatcacgc
     1021 tttttgctcc aatccaacaa tgtttaccta taactatttt attattttta ttcaaaatcc
     1081 tatgagtatt caattcaaaa atcgtatgtg aatcggtatc cataaataat gtatcccacg
     1141 atactgtaca gccatctttg aattgaattt ccttaaagca aattatactc atttttcctg
     1201 tcgaattaaa ctttgaccca atatctaaaa ctgcattttt ccttatgtca atatgagccc
     1261 cttgtgcgat aaacgctggc ccttgtattc ttagtattcc ttcaatactt ataatcgttt
     1321 cttcatttcc cgcagtattt aatactggaa agcccaaata aactattttt ccagattgta
     1381 atactatctc aattttccct ctcgatttgc gtgtaatccg tactttacga tgcaccaaaa
     1441 taggtaatct ccacgcatct gaaatatgaa gaaatttata attgaagtat atcgttttag
     1501 gtaaatttaa tatattcttt attatcaatc cgagtctcat ctttcaccat ctctttttgt
     1561 aatcaaaatt agtttataaa ggattacacc caatataccg cccaacgtat tatatacaaa
     1621 atccgaaatt tgaaacgttc ctagcctcaa aaaaagttgt aaaaattcaa ttattgctga
     1681 aaaataaaat gcaatttttc ctccatatac tattgaggac catattttgg gtttctttat
     1741 attttcatat ttaatagaca ttaccatata aacatatgga ataaaaagaa taatattttc
     1801 taaacacgct gtagataact cttttgttcc agttgttgta ttatacttat aaatccacca
     1861 gtctcccata acatcagata aaggatttaa ccaaattgtt cgattgatta atgttgtaaa
     1921 taaaatcatc gcagtataga atatcaaagc acaaactttt ctatattttc tatccttctt
     1981 taaatgaaga taaagttcca ttagtatacg tttatagccc tcacctgcag caatgccatc
     2041 tgttgcatac ataaaataga ccagtcctaa aattgtcaat aaaacagcaa acccaaaggg
     2101 ttcatataat ttatataata tatctgtaat aatttctgca atcactatta tcataattat
     2161 ttctccaata tcaagtatac acttatcgct tccaattaca catgatcata ttagagtgta
     2221 ttatcacatt ctctaatatt caatttattt ttcctaaaat attcgaaata atcgtcttga
     2281 taacgagtaa gtatttttat acgattgata acatagtaac aatattttta attatagtaa
     2341 aaaatcatct gcctttttca tccacctaat cttatttttc attttattca acatccttaa
     2401 ataaacttcc gttcctaagc tctataatat cttcttaaaa aataatatta tggtgtcgtt
     2461 ttcaaactcg taatctttta cattggaaaa atacatactt ttctcaatgt agcaactcct
     2521 atctcacagt attcaaaaat tcttcaatat tatgaaatac ttgtgcatgt tctttaaata
     2581 ccttgtcagt ctgtttataa tcccctacta aattttgcat tgctcccaac tcttcgacaa
     2641 attgtcatct ccatctgtaa acagccctaa aaactcatat aatgcttttt catcaatatt
     2701 tttcccatat tattctcggt atagtttcac aagttttacg gattcttcat attgtttatc
     2761 tatttataat attcatctag ggacataatt acttttgcta gatttcctgc aacaacacaa
     2821 tcgtctgata cgtccttgtt tactaaacta tttgcaccta taactacatt atttcctata
     2881 tgtacaccct ttagtattct agacttcatt cctataaaaa cattattacc tattttgatc
     2941 ctctccgatg atcccaaagc ttctccatac tacttttaaa accgatcaat cataaacatg
     3001 agtcaaaatt gtaactcctt catatcatct ccaatttcaa caagagtctt cgttggaaca
     3061 aaaactatac atcgttcgcc tattcggcag cctaaaaatt gtagttactt tatatatcat
     3121 tctgatgtct cttctcatct ttttaccaat accgacgtaa gaactttcaa aatatactta
     3181 aacttcttat tacggatatt aaacagaaga ataactaaaa tcgatatgac aaccgatata
     3241 actaaattta tcactaaact tatcactttg aaatttccga tattaacata cgaacaagca
     3301 aaataactta aataagaagc aacaaaagct attacacaca aacttatgag cttccaaaca
     3361 tatttacctg ccgattcaaa tttaaatata ttttggaaaa cattatataa taaccagggt
     3421 aaatcaacca acaatactga aacaatggtt gccgccaaaa ctccatataa tccgaaccaa
     3481 ttaacgagga tgatatttaa tacaatgttt atcattgctg taattaatgg tctaaacctg
     3541 tctgcgtacc atattccagc agcatctttg tataacccaa ttaactgatc aatttcatat
     3601 aagaaaaaat gaactatcat aaatataaca aatgaaatac tcataagatt atcacttccc
     3661 acccagataa tcatgaacgg ctgaaataat gtaataaagc atgcacaact aattgccaga
     3721 aaccaaccta taattgttgt aatatcacaa aaatctatat aatttttttc ttgactttct
     3781 acaataagac tgttacctat acctgctata catgattcat aaattattgc aaatatactc
     3841 attatggcag aaataataaa gtaataattc tgataaatcg caagtactga aagacctaag
     3901 aaagatgaaa taataattga atctgaagat cgtaatattg taccgcctac cttgtttgta
     3961 actaatcctt ttactttttt tataatatcg tcaatagttt ctttctctaa ctcaccaaca
     4021 ggagtataat caggatacat cttttgagaa acaacagcac acattaagtt aagaactatt
     4081 tgtcccaaaa tagaagccaa taaatacaaa tagtaatttt ttaagaaaat cagaacatat
     4141 gcctgcatta caaattggaa tgtaaaagta attagcatag acacgttaat tacatcattt
     4201 ctttgatgtg catatattag actctttcta tatgaaaata accaatacga caaaacagta
     4261 tttatcaaat acattaaata caaaactgtc atatttaaat ttccaggaat cgaaccttta
     4321 actaaaaatc tcaaaaaagg aatgcataac aatcctaatg taaacactat ccatccaata
     4381 attgaaaagt actttttata tgcgttcagt aaagcacaaa tctcttttct attgtcttct
     4441 gctataggtt tatacatact tacagttagt gcagcaccta ttcccaattc cgctaaattt
     4501 aacatttgaa atatagatgt gaacaggttg ttcaatccag catactccat tcccatctga
     4561 taaatcataa gtgtacgcat aaaaaaggga atgataatct gatataactt aaaaattgtt
     4621 ccagtaatta tattcctttt agcattttgt ttacgattaa ttatcatatc atatggctcc
     4681 aaactttcta ttttttattg ttacctaata ctatataatt tctaaaatct tgaaatgtaa
     4741 taggtttctt ctagctcttt gattaattac gctaattttc atgtttggac aaccacaaaa
     4801 aactatacat tttcaatcaa agcaacccat tgttttgtaa tttctttcaa attaaatttt
     4861 tgtacattat tatatgcgtt cagtgacatt ctcattgcta aatcagtatt cttagtaaac
     4921 attatcatgc tcttagctaa ttggtctata ttcttatctt caaccaacat cccattaaaa
     4981 ttatgatgta ccaaatctct cggaccagtt ttacaatcat aagaaataac tggtaatcca
     5041 taagactgtg cttctaatat aaccatagga aatccttcat aaacagaact aagcacataa
     5101 caagatgcag atttataata ttcctccaca tggtctgaat gccctgctat agttactctt
     5161 tgcagattta aatcttttat ctgctggcga agatcttttt ctaattcacc ttttcctaca
     5221 atgataagat cccaatcatc tacttttttt tctacaattt tccaagcact tatcatgtca
     5281 gcaaaatttt tctcttccac taatcgccct actgcaagaa atctcttttt atcataatga
     5341 tattcgtact cgatattaga tatacacgga tttggcatag ttataacaaa atcttctgat
     5401 aaactatatt catcaatatg tttttgacga tcttcttctg tgagaaccac aagcctcttt
     5461 gcatattttt ttatcaattt tttagccttg aatctcttct tttctttcag catttccatt
     5521 gtccagtaat taaaatgttc ccaagaaatc aatttgcact ttgttccttt aattgccctt
     5581 gtagtataca aagacaacaa agtatcaata tcaattacta tatcaaattc atttttgcat
     5641 atatacttgt gcaatttcca aaccggatat aaataagttc tatataattt tccctccgtt
     5701 ttaggattta acatataatc aaccttaatc gtcttatcta acccaaaata aggctctcca
     5761 ctattccaaa agcttaaaat agtgacatca taacctaaat cagacaagct attggcaata
     5821 atactcccta ctctttcagt tcccccagaa cgagaaatta caccactaat gaaacaaatt
     5881 ttcttcaaag tacttttctc ctttataagt gtaaaaattt tttcagtgca agtaacctgt
     5941 taaatttttt ttgtccaatt gttgatttta atctataaac aaatttttgt ttaggtgttt
     6001 tccaagatgc attatattga tgaatgctta ttgtattact agttttataa atttttccag
     6061 tatcataatc cttggcagca aaataatccg taggatagat atgcaggcca tttattattt
     6121 gttcgctgtt atcgcgaacc aatccaatag attcaagata atctgtaata taatacggac
     6181 tcggtttcaa gttcaatgta ccatcatttt gataaaaact taatgaatcg taaatatccc
     6241 ttaaaccttt aataacagaa tgtccttctt ctgcaccaaa accaataccg ccattaaccc
     6301 agatttcccc ttcaaatccc atataggcat gattttcacg aagtatatct aacgacttaa
     6361 ttagctcaac atctgtatct aaataaaatc caccatgatt ataaataata tcaaatcttg
     6421 cataatctgg aacaaaaccc catttacgtt ctttatatgc atcggccata taacggttct
     6481 tatttaaatc ataatttgat tcattccatt caataatctc ataatcaggg caatactttt
     6541 tccagctttc tatgcacttt ctatcttttt tcggaagtgg atttcccccg aaccaacagt
     6601 aatgtatctt cttaggaatc ataatatctc tccttttgta tttttttcac tataataatg
     6661 ctctttcctt ttcacaactt caggcatggc taaaatcata taaatcagtg gccccgttgt
     6721 ttcaccccta agtgttgtgt tatacacaaa aaagaatacc gacataattg caataacttt
     6781 aacaagactt gcatctttat ccttcataaa agatcttttt gcattgaaaa atgtctttat
     6841 tataaaaaat aggaatatcg aaactcctaa tattccaact tctataaata accaaatatg
     6901 atgaaaatac tgataatgaa gattaccaaa cgatttataa aactcactga caaaaaaatt
     6961 ggtaaactct gcattgccaa tgcctactcc cattaggact aatctaaaat tattattaaa
     7021 tatttcatct actactctag agataccagt tattcgattt aaatcaccaa caccatcata
     7081 tccagaatct cttgacgcat attctaaaag accttgaatt gtgaaattat cagcatagct
     7141 ttgtccatta tcgttcaatg taataaatat tgttataaaa aaggataata ctatcgacac
     7201 aagtattacg catgataata tcttgatcac tactttaaaa tcaactttta aagtgaatac
     7261 tattgcaaca aatattataa ataattcagg aataaaaact ttcaattcag ctaatgcaga
     7321 cataaaaagt gctgcacaaa cacataaaat aaaatggatg aaattttttt ctcccttgaa
     7381 atatccgatt gtaacaacag taactgtcac acacaaaatt aaattcaatg gcatattaca
     7441 acctgaataa ttccaaaaaa ctccacctat catatctcct acaattgttc cagttggata
     7501 ggtgaccatg aatctttcaa ttaaacaaag tggcagtgaa atccagaata atttttcaat
     7561 tatagcgaat actttatagt aatctttaat tgttaaatat gttgcacaat ttatatatac
     7621 tatgaacata cgagtgttat ttcgtatccc ccagataatc aatcctatat tttctaaatt
     7681 aataactgag ctacaaatag aaactagtaa aaacaaaact atcagcatct catttttgct
     7741 agcatatatt ccttttctgc gtctaaagct aagtgcattt aataacacaa agatattaat
     7801 tatatctccc aaatataaaa ttgctcttgg ggcgttaaaa gtatatacaa gaactatgga
     7861 gattgcaaaa aatgccaacg tgaacaatgt ccaatctgct cctgaccatc tttttattga
     7921 aatacgcatc gttattcacc ttctttcgct aaaaattatt ctaaaaatac ttttgttaaa
     7981 ttaacatacc ctattatttt tgccggtaca cctgcaagaa ttgcaccatt ctctagacaa
     8041 tctttgataa caatagctcc tgccgcaaca actacattgt tagctatttt tacattacca
     8101 ataatctttg cacccacgca tacacgtaca ttatttccaa ttacaggaca cttaggatcg
     8161 attccatcat ttccaataca gttattccca tataagtaac aattttttcc tattatggcg
     8221 ttaccattaa taacaattcc ttgtgtatgg taaaatctta caccttcatc aaatacattt
     8281 tcaccacact ctataccaca ttttcttccc aaactattca ttcttctgca agacacaata
     8341 tacataatcg cataaatgat atttttcttt ctctttgaat aataataaga tgtaattctc
     8401 attcgacgag tataccgcca agcgttgtag tccggatgtg atttcattat ttttagtaat
     8461 tttgtgtgtt gtcctttaga ttctatatat agattctttt ccatacgaag aaccgctttt
     8521 aaatattctt tactgctaat ttccattcat atctcctatt aattcaattt atacttaaca
     8581 attttattcg gatttcttgc aacaagagca tcattcaatg ctactgaatc tacaacgatg
     8641 gatatgttat cttcatcgtt gattccaccg gatacaatat tacttataaa aatcgtaaca
     8701 ttttttccaa aagaagggat gtccgtgatg tataatgccc attttccgat atttccagtt
     8761 atttaaacct aacgaatttt tacaatagta caagtatcac aatcattaat atataactgg
     8821 aatataaatc tccaaaaatt cactatagta cagtttttta tgattaaaat ctcctatttc
     8881 tacccattcc gcttaaatat gctaatgttt taaccccaat aatggattgg tttagtaaaa
     8941 aatagttaaa tattcgttgt ttaattggat tttgtttata tgggtatgag tgaatacagg
     9001 caatcaactc atcgttcata agtatcttag acaaatagga gtttaaatca aattctttat
     9061 taaaactagc atatctaact gccttttgaa tacaagtacg acagcgtccc aacaaaagtc
     9121 tccttgtttt gtttacaaat atctcatttt ctaatatata gcttaaatcg ccttcaattt
     9181 ttttaactaa ttcaatatta caatcgaact cattatctct aaaattatgt gtcaatgaat
     9241 tattattatt acatctataa taatagatac aatctttaat aaaacttgtt atttttgaat
     9301 gttgcaaata tgaaatatgg aatatgatat cttcagatat ataatccctt tctgacggaa
     9361 acttgatatt gtgctgttga attatctcag atgaatacag cgcatgccaa actgacattg
     9421 gcaatataaa atcgacttta taactcggct cagtgccaat cattccaagc aacacttttt
     9481 caattatagc gttatttgta aatatcgtac tatcatatct tgctggcttt gaaataatat
     9541 tattatcatc ataatattca taatatccag cataacatgt gtctgcatta ctccttttta
     9601 taccatttaa cattgcttca atataattag ctgcaacaaa atcatcagaa tcaataaaag
     9661 caacatattt tccttttaca tgttcaagtc ctgtatttct tgccaatccc aagccagcat
     9721 tatttttatg aataatacga atccttgaat cctttaatgc atagtcttca catattttat
     9781 tagatgaatc cttagatcca tcatccacaa ttataatatc taggttctta tatgtttgat
     9841 tacacactga ttccaaacaa cttcctacat acttttcaac attatataca ggtatgatta
     9901 cactaattag atcactcgaa ttcatccttc tatcctttca ctcacgtttt ctcaattagt
     9961 caatgacggc atcaacgatt ttcttaaatt ctctgaaaaa aatagactct gatcttgatg
    10021 atgtgaatga catactatct tcttctagaa aaattatttc ctttaaatta tttattaaat
    10081 caataatatg atcctcgtaa gtatgctttc ccttggaata atgaaaatat acttttgtat
    10141 tacacaatat tgcaatttta tttcttaata tgattatttc actcattcat tactgccaca
    10201 tatacacaac ttataaccac cccctgcaaa gtttagggta agttatcaga taatatcttc
    10261 ctcttcatca tccagaatac aaaatctatt tatttataga aatatcatta atgtttttat
    10321 ataatgtacc gtgcttgtac tcttgctcta ttacaagcac taaaacacta ctattcattt
    10381 gaattcttac acggataaaa atttacatcg tctatttcaa tattttttct ctctatttta
    10441 ttcacttaat aagttcatta cataatcgaa acatttttta ttttccttgt catcactcct
    10501 tgctcccttt ttgatacaga tttaaaattc tatcaaagaa atttccatcg ttaaatttcg
    10561 ttattgccaa atccttcgaa caacgtgaaa attttacgta tttatcttta tcattgattt
    10621 ttagtattgc agatgtcaac gctgatacgt caccaggttg aaacaaatat ccattttctc
    10681 catcacgtac tgctgaagaa atatctccta catctgtagc aattacaggc attccataag
    10741 aaatcgcctc tagaatagat ataggcaatc cctcgttata ggacggtaaa atcatcacat
    10801 ttgatcttct atagcactcc tccttttttt ctcccacaac ccaacctgta aattcaaaat
    10861 acgattgcaa attatattct tctatcaatt ctttgagcga tatttcttca tcacctgctc
    10921 cagcaatctc aaaaataaac tttttttctg gatattgttc tgcaatgttt ttaactgctt
    10981 ctaccaaatc gtgtacacct tttcgcttta tcaaaactcc cataaacaga aatcggcaca
    11041 tttttgaatt ccactttatg gtatattctg gaactacaat agaattttct acaacaacag
    11101 ttttacaatt tggctcaatt tctttaatta tcttcttcca tttttctcca agtacaatcg
    11161 tggaatcaga ctctcttaac agcaagcgaa tctgattttg tttttttaca gaaacactat
    11221 cataccattt tttaaattcc gatccatgaa gatggatgat tacaggaata tttaataatc
    11281 taaaccactt attcagcaca tatttcctac taaaactccc tctgtacgac atgtgcatat
    11341 gaataatatt aggtttactc aataaagaga tacctataat tttagcaaat gcatacataa
    11401 aatataaaga tttaaatatt gcatttccac ccttataact cggtataaaa gtcatctcga
    11461 tattcttttt ttcccaatta aaattgagca tttgtgtaat tatacttgta attccacctt
    11521 ttaccgagct gtcattacca atcataagta ctctttgcat aacgctttac tccttatatt
    11581 tatatttttt ctattaatac ctctatcgaa ttaataatcg tttccgtgtt agatacatac
    11641 ttgttaaaca tccgaactct tgtttcttta acggcatctt ctaaattttc taattcataa
    11701 caaggataga taatattcat ctcatcaaat tgtctaatta actgcagttg gtgatcatcg
    11761 acatgctcac catacttagc caatcgagga acagcaatga cccttttacc tttttttacg
    11821 gccccaatta tagcacctgt acctccatga gtaatcacca tttctgcttt ttgcatagtt
    11881 tcagtaaact catccctatt tagaaatgct ttataggaat aatttagtgg tttataatca
    11941 ctataaccac tctgtgcaaa tgtactttcg attattactc ctttttcaat taaatcatca
    12001 atagctttta acaatctatc aaattgaaat ttttgtgaac ctaatgttat aaatatcatt
    12061 tttttctcct attatttaat atatcccacc caaataaata gcattaggat aaaattgtag
    12121 catagtttcc cactgaacat aaaattgatc tgcaaaatga tacatcaatt ttcctgtctg
    12181 agtagggctg gttacttttg caaacgattc aatataaatc agtttttttc ccatcatttt
    12241 tgttattaag cagataggga tcatagctaa aacacctgta caaatcacga gatccggttt
    12301 ttcctttata tatataataa ttgatttgaa taaattaata agtatagtac acaaaaaaaa
    12361 cttttctttt ctatttactt gtctcatgta gtacgttttt tcgccttttg actcagctga
    12421 atattctgtc tgttctgtta taacaaaact ctcatacttc tccatcagtg gttttaacat
    12481 cattaactgt tcataatgcc ccccagagga agccgcaaaa catatctttt tgttcatatt
    12541 aacctctcta tcagtcacgc tgaaaaatat ctctagtgta aactttttct ttaatatcat
    12601 ccaagcaggt atcatatcga ttggcaataa tagaatcaca catcgcctta aactgctcta
    12661 aatcgttgat aaccttactt ccgaaaaaag tctctccatc tttcagtgca ggctcataaa
    12721 tcaccacagt cgcaccttta gcttttacgc gcttcataac tccctggatt gaactctgac
    12781 gaaaattatc actgttgctc ttcatggtca gccggtatat accgattaaa actggatttt
    12841 ccctgttgac atcatactga ttatattcag cgtagttgta atagcccgcc atctgaagaa
    12901 cacgatctgc gatgaaatcc ttacgggtac gattagattc aacgatagcc tcaatcaaat
    12961 ttccaggaac atctgcatag tttgccaaca actgcttggt atcttttggc aagcaataac
    13021 ctccatatcc aaaggatgga ttgttgtagt gagttccaat acgaggatcg agacaaacac
    13081 cattgattat ctgctgagta ttcagcccct tcatttcagc ataagtgtct aattcattga
    13141 aatagctgac tcgcagtgct aaataggtat ttgcaaacag ctttactgct tcggcttcag
    13201 tgaatcccat aaaaagagta tccacgttct ccttaattgc acttttctgc agaagtgttg
    13261 caaaaacatg agcagcattt aacaagcgct catcagtcat atcagtacca acaataatac
    13321 gagatggata cagattatca tataaagcct tgctctcgcg cagaaactct ggactaaaaa
    13381 taatgttctt actccccgtt gtctcacgga ctgattcagt gtagccaaca ggaattgtag
    13441 acttgatgac catgatggta tcaggattca cgtccatcac aacctcaata actgactcaa
    13501 cagcagaagt atcgaaatgc tgagtacgac tgtcatagtt agtcggtgta gcaataacaa
    13561 cgaaatctgc ggatttataa gcgctccttg catccaaagt agcagttaag tcaagttctt
    13621 tttctgtaag atacttttca atatattcat cctgaattgg agacttccgt ttattaatca
    13681 gttcaatctt ttcagggata atgtctaccg cggttacctg gtgatgctgc gccaatagtg
    13741 tggcgatact caaaccaaca taaccggtgc cggctactgc gatgttgtac tttttcttca
    13801 caatatctcc tctttcctct cttaagtatg gagaatgtta ctcctacggc tattcatcat
    13861 gtcaaaataa cacagcttag aatgttgata aaataatttt ccagaatgtg ataaaatctc
    13921 tttcatttat aaaccacaca tctaatttta tctattctcc acaagacatg tagagtattc
    13981 tttaataaca agatacgacc ttactaaata cttcaaaaaa ttatcatatt taattcataa
    14041 atataaatct tcattttaaa tgtatgccca aaaatttatc tgtaatgcgt catcaaaatc
    14101 tagattccaa atctttatca atttttatcc actcattcta ccactttagt aatattttct
    14161 atttataaca ctttcgtcct acattgatcc ccccctctga agtacagttt ttactgtctt
    14221 aaataaaatt ttaaaatcta accccatact ccaatttttt atatattccg tgtcaagttt
    14281 tgctacttct tcaaaatcag taatatcact tctaccactt acctgccaca ttccagtaat
    14341 tcctggcttg atagcaattc ttgcacgatg atgataatca tattcttcat actctgattt
    14401 aaggcatggt cgaaatccta taaggctcat ctttcctgtt agaacgttga aaaattgagg
    14461 gaattcatct aaagacgtgg atctaataaa ttgaccgatt cctgtctttt ttgttccatc
    14521 tggaagtatt ttatttccga taattcttgg atcaaactcc atcttaaaca taagattatt
    14581 gctcatctta ttttctttca ttaattctac ttttctttct tctgcatcca tatacatact
    14641 tctaaactta tacatcttga acggtttacc attctgacct atacgttctt gtgcaaagaa
    14701 aattgggcct ggtgaattaa tataaataag tggagcaata aagataaaaa taattcccgt
    14761 tgcaatacat ccaacaatac ctgcaagaat atcaattgtt cgttttataa atgcctgttg
    14821 agttgtcata acattgatac ttgttgtaag tactgtatat tctcctatac gaccaataga
    14881 ttgttttccc aaagcactat ctgatacttt tgcaagattc aaatgtacag taaggcccat
    14941 ttcagagcat cgttcaataa attctttcat acacgaagta cttttattca aaataataaa
    15001 gacttcatct acccatccct gacataagta atccgctgca tttttggaat cagaaacaac
    15061 tgtcactccc tcaacatctt caccaatcat gcttttatct atgataatga caccatttat
    15121 tttatacatt ttatagttat gctctttcac attacgaata actacttcag caatactact
    15181 agatgtaact ataataagtg atttgttccc ctcatttttc attttatgtt tcaagtaatg
    15241 tttccataag aggcgagtta tatatgtaat tacaacgtat aaacctccca tcaagaaaag
    15301 aacgactcta gaataatcat ttccaccctg aacactaatg agatacaaac ttcctaacag
    15361 caccaacata atcgcttgct ttacagaaga agcaaattct atatagtacc ctctttttaa
    15421 tacacctcta tatgcttcaa acatgaaaat aactaaaaga tctgccagtt caatgaagac
    15481 tgccatgttt tgatacattg gaatcacata aggattccaa tctacctggc gtaagacata
    15541 cgatagataa aaagcaagct ggatacacaa taaatctaaa ataataaaat caatatgttt
    15601 aacccatccc cttgaacttt tcttgtacat tcctttttac tcccctcttt ttaataaaat
    15661 aaattgtatt acttgttttc tctcctaaat atctttctga aggattattg actcgacaaa
    15721 ttggcttgtg gatattctta tatatagaac atccgtaagc caacttaaaa ctccttaaca
    15781 aaattttttt actcatattg tagatcaaaa caagtatcca agaaatcata ttttatttct
    15841 tttttctgct tactcaacaa cagctaaaag aaaaatcatc ataaacaaat tcaactaagc
    15901 caacgatccc cttgtgaaat caagtttttt cttgatttac gaataaagag ctaaaataaa
    15961 ccaaacttct tcttttttac ctcttcatag actggataac tgatttcatc cccgttgatc
    16021 agatcctttg cgacttgttt catctgtact actttttcct taccaaagtc ctttgcaatc
    16081 tgctcatagc actcttttaa gtaaaaggtg cgcttagaga ccccatgggc atcagaagca
    16141 gccatctgca cgagattatg tttgaccatc gttttggctg ttttctggat gtctttgcca
    16201 aataccccaa cataacttgg cgcagtgagt tgggccaaac atcccatatc taggaacgga
    16261 agcaaatgat ttgggtcttc gcgaaaatgc acgttgcgtt ctggatggac gattactggt
    16321 gtctttccta gttctaacaa ctcaaaaaaa agccgttctg catataacgg cacttccagt
    16381 gtcggaaatt cgatcagaag atatgtatca tctaaatctg taaataatat ttcatctcgg
    16441 tggatgtctt cgatgagcgt tccggtaata cgtacttctt gtccttccaa taacgtcaaa
    16501 ggcaactgtc ttttctctaa ttcggcttgt aacgaagcga ctagagaaat gacttgtgat
    16561 ttttcattgc tgtattttcc attattgtgg tgtggtgtgc ataaaatatg cgtgatgcct
    16621 tgctgaatcg ctttttcagc cattgcaata cttgcttcta agctttctgc cccgtcatcg
    16681 attcctggta ggatatggca atgtagatcg atcatttctc ttgtctcctc tcttcttatt
    16741 ccttaatttc cgtagtaata accagcatct ttgctatgtt ctgctccgtt gtacacaaca
    16801 cctaagactc gtgcttgtac catctccagc agttcttttg ctttagtcaa ggattcttta
    16861 cgtgacatat tttcacgtac gaccaataaa gtgccatccg tttttaaaga catgatctgt
    16921 gcatctgtta cggctactac tggtggcata tcgaatatca ccacgtcaaa taactgtctt
    16981 gcttcggata ggatctgatc cattcttggc gagcttaaca attctgatgg gttgggtggc
    17041 ttaggtccac taggcaaaat cgacaagttc tcgactggcg ttcgttggat cacatctgct
    17101 acacttcctg atgaactaag tgctgtactt aatccactgg cattgttcag ctggaatgtc
    17161 ttatagacga cgggtttacg taaatctgca tcgactagta gaactcgttg acctgattta
    17221 gcaaaaacga ctgcaatgtt tgctgcagtg gtggatttcc cttctccagg acctgatgaa
    17281 gtcacaacaa tcgttttgat ttgctgacca gctgcggagg caaactggat attcgtccga
    17341 atcgtacgat attgttcagc gattggtgaa gagggattgg tgagtgtgac caaactgact
    17401 gcatgagttt gggctgcttt ttgtttctgt gttcgtgcca ttttctttcc tcctagactc
    17461 ttgagcggct tctgcgtgat ggcgctgttt tatcgtttgt agcttttggt tgtgtctgag
    17521 ctggtccttt tttacggata ccagcattca attctttcgc tgacatttct ggaactgttc
    17581 ctaagattgg gaatcctaat gtatctgtca catatttttc atctttcact gtccgatcga
    17641 gtaattccaa taagaatgcc agacctacac ctaccatcat tcctaacatt aagccagtca
    17701 ttaaattcaa tttattattt ggcgaaacag gtgtcgtatt ggcaactgca cctgaaatga
    17761 tcgagatttt atcaacattc atcacatctt ttgcgttctc ttggaaaact tgtgaagttg
    17821 tgttagcgat ctgttgagca gccactgcat tcgtacttat tgcctgaatc gagaacattt
    17881 gtgaattttc tgcctgatta actgagatgg catctttgat ttgttcagca gtcattttca
    17941 cattgtcttc tgtttctaaa cgatctttga cttcattgag cactaaatca ctgacgatca
    18001 tatctttata cgtattgatc atttgcaggt tcgtattgac atcattgaca tttgcagtgt
    18061 ctgattgtgg cagtgtcacg atcaattgtg cttgtgaact gtattttggt gtcaagatga
    18121 aaaatgttac gattcctgag atggccaagc cagcaaacat tgagatgatg attattacca
    18181 agtgtttgcg taatgtttca aaaatttctt tcaagctgat tgtctcttcc attttttctc
    18241 cactttccta tttattctct agattcattt gatcttttaa ttcttttttc acacgatcaa
    18301 gttcttgatc actgactttt tgataggaga tattatcttg catgaagcct tctccttgca
    18361 gctgatcttg cttaatcgta ccaaatgcct cccgataatc taaagcaatt tttttcattt
    18421 catcaaagtt catgtctgtt ttcatattcg actctagagc attcaaaacg gattgatagt
    18481 tgctgacacc gtttagactc aatacttttt tcgctacgcc ttctacgatc tttctttggc
    18541 gcccttgtct accgtaatca ccatttgggt cttcataacg catacgagaa taagataatg
    18601 cctgatcccc gttcaatgta attttaccga ttggaaagtc atagccatct tgtgagaaag
    18661 tgaggtcatt attgacttcg ataccaccaa ctgcatcaac gagttccttt agccctttca
    18721 tattgataga gacatagtga tcgattggga tgtctacata tttttgtacg gtatccatcg
    18781 ccatggcagc tccgccgaat gcgtaagcgt ggttgatctt gtcttttgtg tcacgaccta
    18841 cgatgtcgac atacgtatca cgaggaatgc tgacgatcgt tgtctgtttg tcttcaggac
    18901 tgactgtggc aaccatcatc gtatctgatc ttccttgatc gacccgacct aaatctccag
    18961 tatcaatccc catcaataga acagagaaag attcttgttt gtccagatca actggtttcg
    19021 ttcgttctcc ttgggttcgt tccactgatt cgtaagtttt ctcaatagaa tgtgagagat
    19081 ccagatataa ttttgctcct actcctacaa ttgctaatac taatacgata agaactgcta
    19141 aaataatcag aaatattttt ttccctcttg attttcttct tcgtcttgcc atagtttcct
    19201 ccgtttgcta accttgcttg aatcaacgtt ttaactgtca atagcttata ttgctatagc
    19261 ccatttgctc agcaacagtt tgtgcttttg ttgcgtatgc tgcgcttatt ttttcattcc
    19321 ggatctgctc tatttgactg agcaccgtca aatattgatc gtaagtagcg gtggcagtca
    19381 cttgaccatc ctttaacatc gtatcgaata actgatctag ttgcgtgact tgcgcgatct
    19441 gaatatcagc gaatcctaga tattgcataa caaccgtttt ccaaggacta tctttaaaaa
    19501 agctcatatt ttcacgtaca cgagcgacat ctgcacttgt caccttttct ttgatgatga
    19561 catcgtctac agcttgctgc cagtttgata catttttagt aaataaatcg ttgacagctg
    19621 tctggatctt cagttttgat tctgcactat ctaactgtgt cacgattgct ttcttctcat
    19681 cttggattgc aacagcttct ttaggcaatt ctgattcttt gatgtcgtaa tcatctgctg
    19741 aaagtttgac agaggatagc ttatttgaca aacttgtgat tgtttcttct gttatttcag
    19801 ggtttagaaa aattttttct tttgtactat acagcttgtc tacttctttt gataaagaag
    19861 tcaaggtgtc tttttgattg gtgatggcag attctgcata attgatccgt tgctgatgtt
    19921 gccgctgatt attgatatgg tatccaaaca aacagatcgc aatagcacct gcacctaccg
    19981 ccacagcagt caatgacttg acgaatggct tttccataga ttcactccta agtattgatg
    20041 atagataaat taagatataa ttcacttaca taaaaatgac agctttttat ttaaaaaaac
    20101 ataattaaac taatatattc acagaaatat ttgtaaacaa gttttcccta tataatcata
    20161 ccacaaactg ttttcttgac tagggcaatt tacgatttaa aagtaaataa aacattaata
    20221 agataaaaat taatcgattt catttttatt tccataattt tcataggtgt ttttaaaagt
    20281 tacacaaaaa atagaataac aaaaaaatta acattgaatt aataatagtt atacccgtgg
    20341 tgctagttaa ttttttccta gaggaaaatg tctaaacatg ttggtaaatc aacaaaaaaa
    20401 cgcatatagt agtctcaaaa ctcccaagaa atgaggacta catatgcgtt catcaaaata
    20461 tactgaacat tatacagata cttttataac attcaaggaa attatgagaa ctgtttctaa
    20521 catataccac aattgtgtac cagataagat taaaaatcga agaaatactg atcaactgaa
    20581 gcaacgcgat acagtgatta ttgcttgtgt tatatggggc ataattaatg gctatactag
    20641 ccaaagagcc acgtatagag cggtttgttc cgtcttattt cctaatggtg actttcctag
    20701 taggagtcga ttcactcgct taagttcaaa cttagcttac actatcaaga ttattcgata
    20761 cttttttatc aagaagctca ccaaaggtga actagtcgga attatagata gttttcctag
    20821 tccattatgt aaaccagtta gaaatagaca agcaaaacta ttgaatcaaa tagccaaagt
    20881 tggctataac tcaacaaaaa aatcttattt ttatggtctt aaaattcata tgatcgttac
    20941 taagactggc tttccg
//
LOCUS       DASHRQ010000048        20525 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.051, whole genome shotgun sequence.
ACCESSION   DASHRQ010000048 DASHRQ010000000
VERSION     DASHRQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.051"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            29..223
                     /locus_tag="U2M88_001900"
     CDS             29..223
                     /locus_tag="U2M88_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374281.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            492..791
                     /locus_tag="U2M88_001901"
     CDS             492..791
                     /locus_tag="U2M88_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374282.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(888..1322)
                     /locus_tag="U2M88_001902"
     CDS             complement(888..1322)
                     /locus_tag="U2M88_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8374283.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1554..2264)
                     /locus_tag="U2M88_001903"
     CDS             complement(1554..2264)
                     /locus_tag="U2M88_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEM8374284.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2254..3114)
                     /locus_tag="U2M88_001904"
     CDS             complement(2254..3114)
                     /locus_tag="U2M88_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEM8374285.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3119..3757)
                     /locus_tag="U2M88_001905"
     CDS             complement(3119..3757)
                     /locus_tag="U2M88_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEM8374286.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3772..4071)
                     /locus_tag="U2M88_001906"
     CDS             complement(3772..4071)
                     /locus_tag="U2M88_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8374287.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4114..5598)
                     /locus_tag="U2M88_001907"
     CDS             complement(4114..5598)
                     /locus_tag="U2M88_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEM8374288.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5618..6079)
                     /locus_tag="U2M88_001908"
     CDS             complement(5618..6079)
                     /locus_tag="U2M88_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM8374289.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6097..7164)
                     /gene="ulaG"
                     /locus_tag="U2M88_001909"
     CDS             complement(6097..7164)
                     /gene="ulaG"
                     /locus_tag="U2M88_001909"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEM8374290.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7382..8152
                     /locus_tag="U2M88_001910"
     CDS             7382..8152
                     /locus_tag="U2M88_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEM8374291.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8425..9252)
                     /locus_tag="U2M88_001911"
     CDS             complement(8425..9252)
                     /locus_tag="U2M88_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8374292.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9820..11055
                     /locus_tag="U2M88_001912"
     CDS             9820..11055
                     /locus_tag="U2M88_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEM8374293.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12054..12314)
                     /locus_tag="U2M88_001913"
     CDS             complement(12054..12314)
                     /locus_tag="U2M88_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEM8374294.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12341..13642)
                     /locus_tag="U2M88_001914"
     CDS             complement(12341..13642)
                     /locus_tag="U2M88_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM8374295.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(13934..14392)
                     /locus_tag="U2M88_001915"
     CDS             complement(13934..14392)
                     /locus_tag="U2M88_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM8374296.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14405..15736)
                     /locus_tag="U2M88_001916"
     CDS             complement(14405..15736)
                     /locus_tag="U2M88_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEM8374297.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15747..17189)
                     /locus_tag="U2M88_001917"
     CDS             complement(15747..17189)
                     /locus_tag="U2M88_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM8374298.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17176..17940)
                     /locus_tag="U2M88_001918"
     CDS             complement(17176..17940)
                     /locus_tag="U2M88_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEM8374299.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18044..18919)
                     /locus_tag="U2M88_001919"
     CDS             complement(18044..18919)
                     /locus_tag="U2M88_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEM8374300.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19174..19258)
                     /locus_tag="U2M88_001920"
     tRNA            complement(19174..19258)
                     /locus_tag="U2M88_001920"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19222..19224),aa:Leu,seq:caa)
     gene            complement(19276..19347)
                     /locus_tag="U2M88_001921"
     tRNA            complement(19276..19347)
                     /locus_tag="U2M88_001921"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19313..19315),aa:Gln,seq:ttg)
     gene            complement(19357..19429)
                     /locus_tag="U2M88_001922"
     tRNA            complement(19357..19429)
                     /locus_tag="U2M88_001922"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19394..19396),aa:His,seq:gtg)
     gene            complement(19491..19575)
                     /locus_tag="U2M88_001923"
     tRNA            complement(19491..19575)
                     /locus_tag="U2M88_001923"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19539..19541),aa:Leu,seq:caa)
     gene            complement(19596..19666)
                     /locus_tag="U2M88_001924"
     tRNA            complement(19596..19666)
                     /locus_tag="U2M88_001924"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19632..19634),aa:Cys,seq:gca)
     gene            complement(19712..19783)
                     /locus_tag="U2M88_001925"
     tRNA            complement(19712..19783)
                     /locus_tag="U2M88_001925"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19749..19751),aa:Gln,seq:ttg)
     gene            complement(19795..19867)
                     /locus_tag="U2M88_001926"
     tRNA            complement(19795..19867)
                     /locus_tag="U2M88_001926"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19832..19834),aa:His,seq:gtg)
     gene            complement(19870..19943)
                     /locus_tag="U2M88_001927"
     tRNA            complement(19870..19943)
                     /locus_tag="U2M88_001927"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19909..19911),aa:Trp,seq:cca)
     gene            complement(19950..20030)
                     /locus_tag="U2M88_001928"
     tRNA            complement(19950..20030)
                     /locus_tag="U2M88_001928"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19994..19996),aa:Tyr,seq:gta)
     gene            complement(20032..20107)
                     /locus_tag="U2M88_001929"
     tRNA            complement(20032..20107)
                     /locus_tag="U2M88_001929"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20072..20074),aa:Phe,seq:gaa)
     gene            complement(20143..20215)
                     /locus_tag="U2M88_001930"
     tRNA            complement(20143..20215)
                     /locus_tag="U2M88_001930"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20180..20182),aa:Asp,seq:gtc)
     gene            complement(20225..20297)
                     /locus_tag="U2M88_001931"
     tRNA            complement(20225..20297)
                     /locus_tag="U2M88_001931"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20262..20264),aa:Val,seq:tac)
     gene            complement(20313..20384)
                     /locus_tag="U2M88_001932"
     tRNA            complement(20313..20384)
                     /locus_tag="U2M88_001932"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20349..20351),aa:Glu,seq:ttc)
     gene            complement(20399..20488)
                     /locus_tag="U2M88_001933"
     tRNA            complement(20399..20488)
                     /locus_tag="U2M88_001933"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20452..20454),aa:Ser,seq:gga)
BASE COUNT         6475 a         4304 c         3265 g         6481 t
ORIGIN      
        1 taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa caaaaggcgt
       61 caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg gttcatggaa
      121 agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc tagaaaaaga
      181 actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat gtatagaaag
      241 gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta gaagaagaag
      301 acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt atttcaatat
      361 gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata tacttgtcat
      421 tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc agtatagaaa
      481 ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag caatttccac
      541 aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag tatttggagc
      601 ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata tacgagatga
      661 aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa aatcatgtgt
      721 tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag gtgagtaaaa
      781 aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat cctcttgttt
      841 ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta tttgtcatta
      901 ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc catttccttt
      961 acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc atctgtcaat
     1021 aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt caaaagcact
     1081 ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat aaatgcgatc
     1141 gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc attacgcttt
     1201 atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt tgtgtaattc
     1261 gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga atcaatattc
     1321 atttttttct tcctttctta tacaattctc cctctaataa tacagttctt tgactattta
     1381 atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt cctctttcct
     1441 tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt cacaacctct
     1501 gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca tctctattgc
     1561 ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa atattgcggg
     1621 atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca aatttcatct
     1681 aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa cggtccatgt
     1741 ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt aaacgtctcg
     1801 acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt cgttaattgg
     1861 cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata agctggcaaa
     1921 tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt aatagcagtt
     1981 atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga tggttttaag
     2041 ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc tgcttgcatg
     2101 cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg gtccacttca
     2161 ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag atttgcttca
     2221 aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc cagcctcctt
     2281 caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt cttggctttt
     2341 ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca aatgtcttaa
     2401 taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact ggcctgaata
     2461 ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat cgatcccttt
     2521 ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta ggtctggata
     2581 tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg aaatggagtt
     2641 cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag aagcttcctt
     2701 tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt tctcttcata
     2761 ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg ccaaatggat
     2821 tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag aaccaaacgg
     2881 aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc tgaacatctc
     2941 ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc gctcatccgt
     3001 ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg aaaaacgttc
     3061 tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag ccatttcctt
     3121 acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc ggatcatttg
     3181 ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca aataagtgta
     3241 atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca atcagttttt
     3301 tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca tcacggcttt
     3361 gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt tcaaaagtcc
     3421 aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc tccatagttg
     3481 gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca acattactcg
     3541 ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta tctgggaaca
     3601 tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt gtgcccactt
     3661 caataatatc tactacatca ccaactttca taacatctcc taatgcatgt tctaagtcat
     3721 tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc tttacttgtt
     3781 tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg acatcacatt
     3841 tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat tcaaaaaatt
     3901 gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg aaatggcaca
     3961 atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca cacttctcat
     4021 catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca tcttaatccc
     4081 tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt ctgcatatgt
     4141 gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt gtaactgcgg
     4201 aatagccaat aaaccaatga caactacggc aattccagca taacctagga ctttcatgat
     4261 tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac caccgtatgt
     4321 tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga ttcctgaaag
     4381 aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa ctgctataac
     4441 ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg atttcaataa
     4501 aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc caatcgttac
     4561 cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa cagctcctgg
     4621 taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga atgtccgaac
     4681 acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca taatgtagaa
     4741 taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt tgcctagtac
     4801 aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca tattctcatt
     4861 gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt gcttgattga
     4921 tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa atataaagat
     4981 tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt cctgcgtgat
     5041 tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc ccataaaaat
     5101 caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa aagcggtagc
     5161 tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg tatatttctg
     5221 aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca tcacatctcc
     5281 aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc caaaataagg
     5341 atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga ttggccgaaa
     5401 attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg ttgcctttaa
     5461 gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta ataacacgat
     5521 aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg tccaaatgct
     5581 taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct gccatcacaa
     5641 cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca tctgtcatta
     5701 ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt gctgcgagtg
     5761 taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa cggattggct
     5821 gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt tctgaagaat
     5881 gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct acacaatgga
     5941 tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca agacagcttt
     6001 cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc aattgatggt
     6061 caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg gaaacggtgt
     6121 atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact caagatcatc
     6181 tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt taaatccata
     6241 cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat cagccataaa
     6301 attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg actccgccat
     6361 gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt caccataggc
     6421 ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat tggagtaatg
     6481 tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt ttactgcaat
     6541 ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt cacaagtgac
     6601 caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga ttttgactgc
     6661 atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt gccacgtgcg
     6721 aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa ctgctgcggc
     6781 tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt ctacattttt
     6841 tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca tattttgaac
     6901 gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat gactacgttt
     6961 tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt tcagccaaat
     7021 tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt ctgtttcgat
     7081 ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact tttcttttgt
     7141 tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt ccaacttaca
     7201 agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg tttgtttttt
     7261 atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag atatgaatgt
     7321 ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg aggcaaaagt
     7381 catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg aactgcttca
     7441 gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat ctattagtac
     7501 gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca attacggtta
     7561 ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg aaaggattaa
     7621 acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc tgtttatcaa
     7681 ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt tgacagtcat
     7741 tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt atattttaac
     7801 tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat taaatacgat
     7861 ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg tagtacatgg
     7921 ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca acaagacaat
     7981 caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa agttcaagat
     8041 tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg aaaaagtatt
     8101 agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt aagaaccagg
     8161 acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg ataaatgaaa
     8221 aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa tcgataccat
     8281 cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat caaacaatat
     8341 cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg gtattcaaat
     8401 cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct ttaccgcgta
     8461 atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta aataatcatc
     8521 ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca atgcataagt
     8581 cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg ctttatcata
     8641 atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga ccatcagatt
     8701 atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt tcgcaaaatc
     8761 taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta agtgatggat
     8821 atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg catctggttt
     8881 tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta aaagatcaga
     8941 attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt ggtattttga
     9001 tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt cttctgtttc
     9061 tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta agatattgat
     9121 gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac aaatgccttc
     9181 tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta atgctccatc
     9241 tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta tatcatacac
     9301 aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt aattttaaaa
     9361 taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt gttatctgaa
     9421 taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa agtctaagaa
     9481 tatttttaaa cataactatt tctttcttac gtgtgtataa tgacgacagc acacctatca
     9541 attaatataa ttattttatt tttttatgtt ctcatgttta tttttaatga cacaacttct
     9601 cgtatttatc tggcacaaca tataaatcga tcaaaaaatt gttgcgtgtt tttttgaaaa
     9661 ttggagtttc aacttgatta caaaatttaa atttagaagt tgtgaaaaca gcgagagaca
     9721 gtttaatatt caaaatatac ataggctact cgaacgctgt gttttttagt atactagtaa
     9781 gaaacgaagg gatagactca ttccaataag gaggatttca tgaagacaat tctttttgct
     9841 cctgcagtat tcaatttagc tgagacaaca cgaatgattg aggttgccaa agctatgaaa
     9901 gaccgttatt actgtgtatt ctttggcttt tctaaaactt ttgctccttt gatcacagaa
     9961 agtggctttg agtatcattt actagcgccg atccttacga aaaaacaaga agaacaaatc
    10021 ataaaatttg accagttgcg tacacttaaa aaccctttta ccaaagaaat ggttgcctct
    10081 cgtatcacta gtgaacagag tctgattgct aagtttgagc ctcgactgat tgtgacaggt
    10141 tcgaatgtga caattttttt atcggcccgc aataaaaaga ttcctttagt atatgttaaa
    10201 ccctatgctt tgagtgaacc tttttttcaa tcagagcatg cgctgatgcc ccacgagtta
    10261 cgtttcttcg gccctttaag caatcccctt tggcactgga caaaaaagcg tatagtaaat
    10321 acccgatgga tccccaatgc cttcaaacac gctgcacaaa aagaaatgct tcctttacca
    10381 aaaacaagtt tacaaatgat ggatgctgat ctaaatctca tcacaacgcc taaactattt
    10441 ttggaagaag aacagctgcc ggaaaattat cgaatcgttg gtccaatttt tgctaagctc
    10501 agtacttcct tgcctgaaga agtgctaact tttatacagc gaaagcaaca agaaagaaaa
    10561 cgtattatct atttcgcaat gggtagttca ggcaatccgc gtatgttacg caaaattttg
    10621 ttcttcttac aaaaacaacc ggatctagcg gttattgcac ctattgacta tctgcttgat
    10681 gacattcaat ctgactcaga agatctttta ataacagaat atctgtctag tcatcttttg
    10741 catgaattca ttgatttttc ttttatccac ggcggggaag ggaccgtcca aactgcctgt
    10801 gaatccggca aaccgtttat cggtgtgggg atgcaatttg agcaaagctg taatattgac
    10861 tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac cagtgactga aagaaaactg
    10921 cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc agcaggcacg cacgctgcaa
    10981 gccgtttttc caataaatgg tccacaacaa gcaattcaag aaatcgaaac cttcctgagc
    11041 aaccacccaa tatagtttca agtagaaaaa gcttttttaa tagagactca tcagtaattt
    11101 cgctattcga gacccagaca gtgaaaatac aaaaaaagta aatagtggat tgaaaagtta
    11161 gagttactta aattacggtg tctaaaaaac acaaaatcat ccttaaaata aaagatctga
    11221 cgctatccaa atgagcatag gtagcgtctg ccatttattc ttagtcaaat tctttgacac
    11281 ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa agaccataat aattaggcaa
    11341 atacattttc ttaaaatgtg tcatatagat ctagaagcat cgctttgtag agatttgtgc
    11401 cgctagtccc ctcaacaatt actaaatcaa atgtaggatt taccacttta tctttgataa
    11461 tcaacctccc tcttctgttg agacaatgat ctcgcaatcc gatgcatact cctatataaa
    11521 ctgccagcaa tccccacata ttgagtagat catgtcagaa tgttctatgt cgtcagcaac
    11581 gatacactaa aagtcacgtt caccatgact gattgctatt cccagcacaa ttatttctat
    11641 acacgtttca tgatttgaat aaacactcac atcatacata ttaccattca tacaccatac
    11701 tgcagcccct actatatggt tgtaagctcc caaatctata tttgacttga tcactttggt
    11761 agtagatcta ctaattctaa atccattgct gataaaattt ccattttttc ctccttcttt
    11821 ctgccaaaac agttaaaaca ataataatct ttatgtaaaa aagggctgtg acaaaagcct
    11881 tgtcacagtc ccatattccg aataaacggt ggtcaaaagc ttgcatctgc ggtaataagt
    11941 tcaaccaaac gaaaaataag ccaacatttc acaaaaattt aagaagcaat tttcgtaaat
    12001 ttcttcttat ttcttgaagc taaccacttc tgttacaacc acttttagaa catttaaagc
    12061 ttcgatgtga caaaccatct atgctgctcg atcataaaat tgatagcaaa aattccacaa
    12121 agtacatatg tcccccatga aaacaaaaca ttcatatgcg gtacaatgat ctttggaaga
    12181 atcacgatca aaaatgatgc cagtgcccaa atccatcgat tgatttgaat atgatatgta
    12241 gctagcatat tcaatataag gtgactagcc gttaataaaa agataaaaac aaaaaataaa
    12301 atcagccaat acattgtttc ccaccttcta aaaaccaaac ttagacagtc tggacttcgt
    12361 tttctccacg actttgttca tctgacagca tctttttatc ataagctttt acaaatggat
    12421 aatagataat aaatgctatc aatgtgttga tcatgatcaa gataacaaca cgccagtcat
    12481 ttcctgctga aagataaccg ccgattggag ctggtaatgt ccatgttggc aaaacagtaa
    12541 atctattgac catttctaat ttgactgcaa aataactgat aatagtcaaa actaaaggaa
    12601 ctaagttgaa cgggatgaac atgtaaggat tcaacatcat aggagctcca aaaattatgg
    12661 gctcattgat attgaagata ccaggaataa tagaaaaacg accaacctct ttcaagaatt
    12721 ttgatttaca gaagaatata aacaaaacag ataccacgat cgttgctcct gcaccaccaa
    12781 ttgttaccgt ccattgataa aactgttcag gagcgatata gggtaattgg gtggcagctg
    12841 taccatctgc caatgccttt gcattctcat ccaacatgat cagccacatt ggacgaacaa
    12901 cagcccctac aatactcatg ccatggatac caaatgacca caacaaatgg atcaaaaaca
    12961 ttggtagtaa aacaccaagc aaattattcc ctaaaatatt ggttaaaggc tcgaataacg
    13021 ataataaggc agcgtttaca tcaaagcccg cgacgcttcg aatcagccaa gtaattgtta
    13081 aaatgactgc acctggaatc aatgcctcaa aactacgggc aacagcaggt ggcacttgtt
    13141 ctggcatttt gatagtaata tttcgatttt taaagaaacg atatacttcc actgcaaaaa
    13201 tcatagataa gatccccgca aacatcccag atgctcccaa agaactcata ggtaagaccc
    13261 agccaagagg tgcctcttca ggagttaaaa cgtttacagg aacttgtaac ataaagaacg
    13321 ttcctaaaga taatatacca cctgtcacac tatctaatcc atatgattta gctagatccg
    13381 aacccataac aaaagttgca taaatagcca tcaatcccat cgtcatccta aagggtaaaa
    13441 caatcgttgc cgaataaggg gcaataaatt cattccatcc aggaaacggt aaattgataa
    13501 agaccataaa aaatgtccca atcaaaatca atgacaatgt tgaaacaacc ccattacgaa
    13561 ttgctaataa atgacgctgt gtaccaattt ttgccattgg tgggacaagt ttctcttcga
    13621 cccatttcat cagcttttcc actaaaacca ctccttttca ataaaatatg aaccattcat
    13681 tgcatgacaa cgcaaagaat gttcataact aattatataa ttaagaaatt ttatttcaat
    13741 atattctctt aaataatcaa atttattttt aagaatcctg aaaataagtt ttctaaatca
    13801 taggagcaac aaaaaaattc gataattaac aaaaatcaaa atgattaaat ctagtgattg
    13861 aaacatatca tttcaaaaaa attttgtaaa ttcacccata aatatcaccc tatttaactt
    13921 ttttcatcgt acactaaaat tcaaaacaac tatctttaat ctttacttct tggttttgtt
    13981 ttaataaatt tccttcaaaa atgcctggag aaactacaat gatcaccgta tctttttgtg
    14041 cttgctccaa cttcttcaaa tctataatag ctaacagtgt cccttctttt acctcctggt
    14101 tactttcaac ccgtatctga aacggacttc cttcgagttc aactgtatct aaacccatat
    14161 ggatcagtat ttttctgcca gattcacttt cgatcgttat ggcatgtcgg gtaggaaata
    14221 tattatcgat tattccttta actggcgcat ggatatatcc atcatgattc gttatagcgt
    14281 atccaactcc tatcattttt gaagaaaaga cctcgtcttt tactgctgtt aatggaataa
    14341 tttgaccact agctacagct tttagaatgt ttcttgtttt aaagaaatgc actttttatc
    14401 acctctactt taatgctggc caatattttt tgtttacttt gatcatttca tctagtattt
    14461 tttttgcgac tctggcattt ggtacggttt gattcaatgt aaatgcttgt aaagcttttt
    14521 gataagaacc ttcaaagtaa gcatctacta gtaacttctc tactgcaact tgtgcctcca
    14581 tcaatccttt atgaaaatca ctgattggtt ttcttagtgt aattggctcc acaccagttg
    14641 caccaacata tgccggtatt tcaacaactg catctgaccg tagattcgga atcgcccctt
    14701 gattaggaac gattagcata aagcgacgac gatcatcatt caacaaggaa atagctatat
    14761 cgacgatata ttgtccatgc tccccaaaat taaagttcaa tacttcttcc acaataccgc
    14821 ggcgtatttt atcagccatt tctttggtat ttttctctcg tccatccatg accatattag
    14881 cacgtgtata ctctttgtca gtatgctcaa cgaccatatc aggatataag taatactcta
    14941 agtaattatt cgggatatta cttggaaaat ttttcgtgat gactgacatc atttgaaagg
    15001 ttttttgcca agttttatct cctatattaa aatcggcgac ttgcatttct tttgttatta
    15061 gtttttcaat aatctctggc ataatttctc gttgcaattc tttatcataa atcgatgtat
    15121 accagccaaa gtggtttaaa ccataatacg taacgatcca attcttacga tcataaccgt
    15181 aattcactgc aatcgtttct tcaattgaaa tagtcatatc acaggcatta atcattttta
    15241 cattcggata tttacgacga acagcttcag aaacgatcga ctctggattt gtataattca
    15301 aaatccatgc tttgggggca tagtcttgta tatgatcgac aatttccaat aaacctttga
    15361 ttgagcgcat cccataagca aatcctccga gtccacacgt ttcttgtcct actaatccat
    15421 gtttaagtgg aatcttttcg tccttttctc gcatctccaa tcctccaaca cgaatttgtg
    15481 agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc atccgttgca cgaatgcgaa
    15541 tatttgaaaa accttttctt ttcagcataa atttaacaat caagtacata tccatatttc
    15601 tttcttcatc aatatcatat aaacgtattt cagataatgg caaccgtttt tcattgttca
    15661 aaatggcttg aacgattcct ggtgtatacg tactcccgcc tccagctata gtgataatct
    15721 gttttttcat catcatcttt tcctttctat tcttctaata atttattgaa ttctgccata
    15781 acataagtca cttctaagcc gatgataatt tgataatttt tttcagttgg tttaatgatc
    15841 ccgcttatcc ctaattgctt gattcgctgt tcctcgacca tggtgggatc cactacctct
    15901 acccgtaggc gtgtattaca actatagaca tccaggatat tggcttttcc tccaaacgct
    15961 tgtagcattt ttgttgccat atattcataa tttttacttg ctaaccctaa ctctgtttct
    16021 tctaaggtta cttcattgcc aaactctgcc ttagcttcac gtcctaatgt ctgaatgttt
    16081 ctcttattga tcaaataata aaaagtaacg taatacaagg caaaaaagaa aattccgatt
    16141 ggaagaatca aaatagcatt ggtagcgatt cgaaaattga tgagataatc aacaatacat
    16201 gctccgaatg taaaaccaat tcgtatattg aataagtaac agacaatccc tgctaaaccg
    16261 gcaaaaagtg catggatcac gtacaacata ggagaaagaa acatgaaact aaattcaaca
    16321 ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat tcctttgacc
    16381 atctctttat tttctttata agctgcttta tagatagcta gacatatagc tggtatacca
    16441 aacatcatta ctacaaaaaa aactgataaa aacagtccag cctgaggatc tccgttgata
    16501 aagcgtgtca tctctcctct gaaaacttcc ttgctaggag atgtatagct tcctaaatcg
    16561 taataaatat acgtattcaa cacatggtgc agtccaaagg ggatcagcaa acgattcaaa
    16621 aacataaata tccccacacc taatgctccc atatcaacga gcagttttgc aaaattatca
    16681 atcccaattt ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat tgtttgtaga
    16741 atcatcacca tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac tactggtaat
    16801 ttttgttctt taaagcgatt atacgtccat gccgctacta aaccggaaat aattccacca
    16861 aaaatcccca tattcacttc tgggtccata atttctaagc cttttttcaa ggtagcaatc
    16921 gctaaaaaac cagtaagtgc aggtattcct ttgtccttat ttttgacaaa accgattgca
    16981 actcctatag caaataagat atccaagttt ccaaaaacgg catttccagc tgcaccaatt
    17041 actgggatgt tcagcatatc gtcagctgat aaacgcatga tcaatcccgc taatggcatc
    17101 gctgcaactg gaagtagcat cgctcttcca agcttctgca catttgattt aacattcatt
    17161 tttattcctc ctctattaaa tatcctcata tgttactaac tcaatcaaag gatcattttt
    17221 cactttttga gcaaatgctg aatgaattaa aaattcgact tggtgggtac gaggatacac
    17281 aaaacttgaa ccatttaaca attcctgatc caaatacgct gcgtgacaca tgacttcgat
    17341 cgttttatct tctctcaata tcatgagtaa acttgccaaa tattcttcca cactcgtatt
    17401 gatttgtcgt tttttttcat cagtttctcc tacgacatcg aaacaaggct caaaataatc
    17461 gacatgacga acttcatctt ttcgttcaaa gttgctccga accggcaaat catatttttt
    17521 tgctaatgta acaactacct cctgattttc atccaaagta tgagtatgat gatgactgtc
    17581 taaatgagtt ggtatgatcc ccgcatgata aattttttga atctgagcat tccattcctg
    17641 atacagttga tctttgtcaa tgttaaatgg atgctgataa taagataagg atctgaactc
    17701 tccgctttcg ataagagtat cgacagtttt gaggagtggt ttgccacacg tcaatgttaa
    17761 atggactcct acacctagcc taggcatttc ctttcttagt ttcacagcat gctcgaaacc
    17821 cggcatattt gccatcaatg tagttgatgt caaaattcct cgctgataag catccataat
    17881 cccataattc acaccatggc tatagccaaa atcatccgaa ttaaaaatca cttttcccat
    17941 ttcattctcc ttcttttttc tactaaaata ggcgcaacca aataatcata gtccatttac
    18001 tatgtgtcat ttggttgcgc ctatagatag ttacgccctt attttaatag attcttgcta
    18061 gatgaattgc caaatagatg atttcatttt cttgaatggc aagttgaaat tgctgttcga
    18121 tgatatcttt aatctttagt gctgtacaat acgctttgct atgtttaaat gtttcagtaa
    18181 aaaaaccaat attttctact ggcgtttctt cctgataaat caagcgttta accaggaatt
    18241 tgatatgaac cattaagcga tgataagtca gtgattcttt atcataaacg tttgaatagg
    18301 atttttctag atgggacaaa atcgtggtcg tcatttctac tatttttaag ctctttaaac
    18361 tattaggctc tcctagctca gcattaatga tatgcatagt gatgaatcct acttcatttg
    18421 accctaactc ggtttgatat cgatgattga ttcgctcgac cacttctact gctatatcat
    18481 attcatttgg ataaaggaat ctcatttctg gcaacataat tgctgtgaca tctctatctt
    18541 ctctttttcg ttgaatagca aaataaatat gatctgtcaa tgtaatatag atatttgaat
    18601 ctaatggaga ggagatccta gctgttatca gttcaatcac ttcttccgca aaatccataa
    18661 attcgattgg aatctgcgag actatctcac tgaatcgttt gatcttttct ttagaatcta
    18721 atacaaataa tttgtcagca tcctcaacat caaatggctt actcacttta gcagaaaagc
    18781 caatcccttt ccccatcgca attttttcta ttccatcgtc atcgatcaat acagcattat
    18841 gattcagctt tttcatcaca tacaccgtta attcacctcc tctatatgat aaaacaaaac
    18901 aacttgcaga ctccttcata catatttttg tctccaaaca atttttaaag acaaaaaacg
    18961 acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc ctacataatt
    19021 tacaaaaata gtttaacaca taataaaagc gttatcaata tcttttttac tcgttttatg
    19081 ataatacttg tatcgaattg atttggaggt aattacataa aaacaaaaaa aatatagcaa
    19141 ctctctataa gaaagttgct atattgataa ttataccggc ggccggggtc gaaccggcac
    19201 gtcctcaacg gacactggat tttgagtcca gcgcgtctgc caattccgcc acgccggcaa
    19261 atttaaaaat aataactggg gtagctggat tcgaaccaac gcatgaggga gtcaaagtcc
    19321 cttgccttac cgcttggcta taccccaaga ataaagggcg aatgatggga atcgaaccca
    19381 cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc atcataaaag
    19441 atgttattta atttctcaat aaaaaactaa gatgaagaca tcttagtcaa taccggcggc
    19501 cggggtcgaa ccggcacgcc ctcaacgggc actggatttt gagtccagcg cgtctgccaa
    19561 ttccgccacg ccggcaagtt gatttattag tactaaggcg gtaaccggat ttgaaccggt
    19621 gatgaaggtt ttgcagacct ctgccttacc acttggctat accgccatta tactattatc
    19681 aatctataaa ataaagaata aattatagaa actggggtag ctggattcga accaacgcat
    19741 gagggagtca aagtcccttg ccttaccgct tggctatacc ccaagaataa aaagggcgaa
    19801 tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac cacttcgcca
    19861 cattcgccat ggcaggggca gcaggaattg aacccacact aacggttttg gagaccgctg
    19921 ttctaccttt aaactatgcc cctataaaat ggaggagagt ggattcgaac cactgaaccc
    19981 taaggaacgg atttacagtc cgtcgcgttt agccacttcg ctactcctcc atggtggcgc
    20041 gggacagaat cgaactgccg acacacggag cttcaatccg ttgctctacc aactgagcta
    20101 ccgcgccata ttttaaaatt attaattgaa taacaagaac gacggttccg acgggaatcg
    20161 aacccgcgat ctcctgcgtg acaggcaggc atgttaaccc ctacaccacg gaaccagttc
    20221 attatggagg ttaacgggat cgaaccgctg accctctgct tgtaaggcag atgctctccc
    20281 agctgagcta aacctccaac attcaattaa aatgacccgt acgggattcg aacccgtgtt
    20341 accgccgtga aagggcggtg tcttaaccgc ttgaccaacg ggccaggtaa atttgctacg
    20401 gagagtaagg gattcgaacc cttgagacag tttggcacca tctacatgat ttccaatcat
    20461 gctccttcgg cctctcggac aactctccag agtgaaagaa gctcaatact ctttctcaaa
    20521 atgag
//
LOCUS       DASHRQ010000049        20521 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.052, whole genome shotgun sequence.
ACCESSION   DASHRQ010000049 DASHRQ010000000
VERSION     DASHRQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.052"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(434..1747)
                     /locus_tag="U2M88_001934"
     CDS             complement(434..1747)
                     /locus_tag="U2M88_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEM8374301.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFMGFMWILNVK"
     gene            complement(1992..2216)
                     /locus_tag="U2M88_001935"
     CDS             complement(1992..2216)
                     /locus_tag="U2M88_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374302.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(2236..2658)
                     /locus_tag="U2M88_001936"
     CDS             complement(2236..2658)
                     /locus_tag="U2M88_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEM8374303.1"
                     /translation="MESVYRQERKSNRKKGQTMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGVLTFNFSDPEGNYFVIGKAESSD"
     gene            complement(2800..3510)
                     /locus_tag="U2M88_001937"
     CDS             complement(2800..3510)
                     /locus_tag="U2M88_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374304.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGDESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(3528..4024)
                     /locus_tag="U2M88_001938"
                     /pseudo
     CDS             complement(3528..4024)
                     /locus_tag="U2M88_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(4061..4513)
                     /locus_tag="U2M88_001939"
     CDS             complement(4061..4513)
                     /locus_tag="U2M88_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM8374305.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(4648..5253)
                     /locus_tag="U2M88_001940"
     CDS             complement(4648..5253)
                     /locus_tag="U2M88_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEM8374306.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(5703..6323)
                     /locus_tag="U2M88_001941"
     CDS             complement(5703..6323)
                     /locus_tag="U2M88_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEM8374307.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(6325..7194)
                     /locus_tag="U2M88_001942"
     CDS             complement(6325..7194)
                     /locus_tag="U2M88_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEM8374308.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANKAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(7217..7624)
                     /locus_tag="U2M88_001943"
     CDS             complement(7217..7624)
                     /locus_tag="U2M88_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEM8374309.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELHENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(7643..8932)
                     /locus_tag="U2M88_001944"
     CDS             complement(7643..8932)
                     /locus_tag="U2M88_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM8374310.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     ILLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(8959..9261)
                     /locus_tag="U2M88_001945"
     CDS             complement(8959..9261)
                     /locus_tag="U2M88_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8374311.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(9286..11379)
                     /locus_tag="U2M88_001946"
     CDS             complement(9286..11379)
                     /locus_tag="U2M88_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEM8374312.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLEEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(11868..12596)
                     /locus_tag="U2M88_001947"
     CDS             complement(11868..12596)
                     /locus_tag="U2M88_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEM8374313.1"
                     /translation="MGLCLFALPTQGFAVAGGHGGGTSSGRSSGRGSFGGRSYGGSYF
                     GSRSYGRDSYGYYGPSTGPSSYEQTGFLALAVLSVGGVSAFSIVKNKRQKKRLKSEIL
                     SRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFAEHQKVLQKNKAD
                     GVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLISGSKTQRQYFSQ
                     IWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(12742..15363)
                     /locus_tag="U2M88_001948"
                     /pseudo
     CDS             complement(12742..15363)
                     /locus_tag="U2M88_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298916.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(15477..16808)
                     /locus_tag="U2M88_001949"
     CDS             complement(15477..16808)
                     /locus_tag="U2M88_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8374314.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRVMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKANFGQLNELVPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQSGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFVGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(16774..18252)
                     /locus_tag="U2M88_001950"
     CDS             complement(16774..18252)
                     /locus_tag="U2M88_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8374315.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(18557..20392)
                     /gene="tdc"
                     /locus_tag="U2M88_001951"
     CDS             complement(18557..20392)
                     /gene="tdc"
                     /locus_tag="U2M88_001951"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEM8374316.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEQVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
BASE COUNT         5929 a         4407 c         3442 g         6743 t
ORIGIN      
        1 atggatgttt ctattttttg cttaatcatt tattttagtc tctctcacta aaaattgtaa
       61 cgctttcaaa tagctattgc accttgttat cggtaacaga ataaaacaaa aaaacagctg
      121 aggaaattcc ctcaactgtt ttttgaaagg attgatttat tcagggaaga atgctgattt
      181 cacaacttct ttataaacgg tgttcactca gctgtccctc ggtattagct caaaaaacgg
      241 caaaatataa gaaactattt ttttcgtttt tttctctaat actcaggcca gaacactgat
      301 ttcacaacct ctttataaac ggtgttcact cagctgtccc ttggtattag ctcaaaaaaa
      361 ggcaaaatat gaggaactat tttttcgttt ttttctctaa tactcaggcc agaacactga
      421 tttcacaacc tctttatttc acattcaata tccacatgaa tcccatgaat acaaagaaca
      481 gaccatacat gatgggagat acttcttttc ctcttttagc tgctaacatg gtaattggat
      541 agaagataaa acctaatgca atcccatctg aaatactata agtcaaaggc atgccgatca
      601 agatcaagaa tgaagggatc gcaatctcta agtttcccca ttcgatttgt cgcaaagatt
      661 gtgccatcaa tactcctacc ataatcaatg ctggtgcagt tacttgtgaa gtaacaactg
      721 atagtaatgg agaaaagaac aagcctaaca agaagaaaat ccctgtagta atagctgtga
      781 tacctgaacg tcctccaacg gcgataccag ctgatgattc tacaaatgca ccaacaggag
      841 aagtacctaa taaggaaccc gcaagcatag ctgttgaatc agaagctaaa gctttcccta
      901 cacgaggcat cttgttgtct ttcatgaaac ctgcttggtt tgctaatcca actaaagttc
      961 ctgctgtatc aaagaaagtc acaagcaaga atgtcagcac tacgacccac atttgaagag
     1021 aattgatgtc ccctacatgt tttaatgcaa ctaaaaatgt tggtttcaaa cttggtgctg
     1081 cggcaacaat ttcagaaggc attggaatca aacctgtgac cagacctaaa atcgttgtag
     1141 ctgccatccc gatgaagatg ccacctggta cacgccgaac aagcaagata gcagttacga
     1201 ctaatccaaa aatcgtcaac caagtagaac caacgttgag tgatcctaat cctactaatg
     1261 tagattcatt cgaaacgatg atcccacctt cgctcagacc aaggaaagca ataaatagac
     1321 cgataccgcc agaaatcgca tatttcaaat ctgctgggat cgcatcaatg atcatttccc
     1381 gcagcttgaa gattgtgatc aaaataaaaa tcaaagaagc aacgaatacg ccggctaatg
     1441 ctgtttccca tggaatcccc attcctaaac aaacagaata agcaaaaaaa gcattgatcc
     1501 caagagcagg tgccgttcca attggataac gggctaatac ccccattaaa atacagccga
     1561 tcgcactggc taatgcagtc gcagtaaaca ctgctccttc atccattcca gaagcaccta
     1621 atacagttgg atttacaaat aagatataag ccattgagat aaatgttgta aatcctgcca
     1681 gtacctcacg ttttaccgtg gtgttcaatt tttctatttc gaagtaggaa agtatttttt
     1741 ccttcatctt ccatccccct aaatccgaac attataaata aagtccgtta aaatttcgat
     1801 ttattaataa aacaacgtgt tttatcatat actaaaacaa aacataagta aacaaaaaat
     1861 tcggatttta agcaaaatcc gaacgttcca tctgattaaa tccctttatc tcaaagttca
     1921 tgaacattca ctttggatta caagaatttc tttattgtgt taataaagtt tcttcattct
     1981 agtctcaaaa atcaatagtg tgatttgatt ttaccatttc gtttctgttc ttctttgatg
     2041 aattcttttg tatcgacagg attcagatta tttagtccgc caaaacctgt atcgaccatt
     2101 tttccccagc cttttgtctt cttttttcct gatttgagat tctttgctcg aagcttctca
     2161 ctatgttttg ctcgccgata cttcaaataa ccttctaatc ttcccattag atccactgtt
     2221 tctcctcctt tttattcaat cagacgactc agcttttcct ataacaaaat aattgccttc
     2281 agggtccgaa aaattgaaag tcaagacgcc attgttgtct gtgatttctc ctggttcttc
     2341 catgttctcg tagatagttt caaattcatc cgtaaaaaat actaaagaag gcggtggtcc
     2401 taatacctct ggtgaaaact tctgcacaaa tacttttggg aatacgccta attctaattc
     2461 ttttgagatg ctgagtacaa tactcttaaa ttcttctggc aattcgattg tttcagaaat
     2521 ttctgcacca attttttcta caaagaatcg gcaaatcatc tctacgtcat tgacataaag
     2581 catcacacgt gttttttgta tcatcgtttg acccttcttt ctatttgatt ttctttcctg
     2641 tctataaact gattccatta tatcacgaaa gcgcaaacac cttgtgtttg atactattca
     2701 agaaaaataa agcaatagtt tctaggattg ctttcctcca caaacttttt ttacaaaaaa
     2761 aaaacggcaa tgaatgccgc tcttcttatt tcgattcttt tataattctg cttctgattc
     2821 atctttggtt agtgtgtgtc taacactagt tacgccaaat gtttgatcga ttttttcctt
     2881 caacgcttcg attgcctgtg cgccacgtgc cgcttgttca gcattgatgg attcaatgac
     2941 cagcaccgct gcttgtgttt cttccgtcag catcgttctt tgggcttcac gccagttgag
     3001 acagcgacag atcgctcctt gttcatcata gtagatgatt tcttccggaa gtgccggagc
     3061 atcggaatct gcacctaaag gcaggaatga ttcgtcacct tgggcttttc ctaaatgcag
     3121 atttcctgcg atcttttcga tatcttctcc gccgcaaggt acgccaaaag acaaggaaat
     3181 actattatag atatctacaa gcggattgat tgggaaaaat tcccgttctt gatttgcacg
     3241 tttcagtaaa gcttcaatag aagaacgtgc acctttttta gtcttgaatg tggtaaatgc
     3301 ctgacgccac tgagcaatga cttcgttttg gctgaatgtt tcttctgtaa ggtaatttct
     3361 tgcttctttt gctgcctcat tcaataattg atgatacgtt tcttcacttt cttttgttac
     3421 gtggttatcg ataccattta cggataagat ttcgatggtt gcttcaggaa atacttccca
     3481 gaaattggta tccactatta cttttttcat ctgttcacac ctcttgatta gaataattgc
     3541 ttgatgaata gatagtctgt ttggggatca tctcccatat aaaaaacatg ttcgctgtat
     3601 ttgataaatc cttttttacg ataaaaagcg agcgcaacat cgttgtgttc ccaaacaccg
     3661 agccaaatgg ctgttttttg cttttctttt gctttttcaa tagctgtatc tagtaaaaat
     3721 gtaccgatac cttgatgttt aaattttttt cgtacataga ttcgctcgat ttctaaaaaa
     3781 tgatcagcca tttcctcaga ttgactcgtt cctgtattta gtttcaggta acccgcaggc
     3841 tcttgatcga tactcactag gaaaaaatct gtttctttat cttttagctc ttctgtaagt
     3901 ttttctagtg cataagcttg tgtcaagtaa tcagctaaat gctccggtaa ttatcttttc
     3961 caaatgtatc tgtaaatgtt tcgatactaa tttgttgtag taagtctaca tctgaaggcg
     4021 tcatttttcg aatttcgtat ttattcacgg cttcttcctt tcaatagatc cttttattgc
     4081 cttttttgac aaaattccaa tcttcgctaa cattcttgca tactttattt aaaagatatt
     4141 ccaattgttt tgcttcttcg tcagaaagac catttaatgc gactgcattg gagtagttgt
     4201 tttctcgaat gatgaaagga tagatttctg ctcctttttt agtcggatag agtttcttga
     4261 tttttttatt ttctttatct tccaaacgtt cgatcatccc attggattct aatttcttga
     4321 ttgctcgggc agtcgttgta cgatctactt tgatcatttc tgctagtttt tcttggatga
     4381 ttccaggatt ttcgcaaata cgtactaaat aaagatactg tcctcttgtt aaatcgactt
     4441 ctttgaattc gatattgcta atagagtcta gcgctcgggc aatcattccg atgtctcgaa
     4501 ggatttcagc catttcatcc cacctttatt ttgtttgtaa gactagttta cctctttttg
     4561 ttgcaaatgc aactattttt aataaaaaaa tcaagttctt ttttaataac tagtaaagta
     4621 tattcttttc ttcttttacg aaaaagctca atcacaatag atctggtttt tgttttctaa
     4681 aataagtaac tgtttcttcg ttgttcgctt ttctgatagc tttggcaact cttctatact
     4741 aaaaaatccc atgttggaag tttcattatt ctcaataaac ttggcctctt caccgtggat
     4801 cgcacaagca aaaacaagtt tataatactg aaaaagctgt ggaatatctt tttgtttatt
     4861 cgtatcaaaa actgctcgca actgagtagt ttctactgtt aatcctgttt cttcatagac
     4921 ttctttacgc acattttctt caggagacaa tcctatttca gcaaatccac caggaagtga
     4981 ccattctttc gtccgtttat cttcgattag taggaccttg ccatcttttt tgatataggc
     5041 acgaacatct acttttggtg ttggatgtcc ttctccaatc gttgtgagcg cttttatttc
     5101 ttcgaaagtt tcttttccta tttcactgat cagttccaat gaaatcgtcc ggatctcctc
     5161 aaaacgctct ttatcaaata catcttttcc ataatataga ccagcttgag aaagtgcgag
     5221 tagtcttttg tattgagcca acttcttgtt cattctcttc ccttcttttt tagcaaatgt
     5281 atgactatgt aatcaagtat agaagactgg tttaattacg gcaaataaat ataatgaaat
     5341 ctgcaataaa taaaagaatg ttaaaaaact cgagaacaaa agacattcga cttctctggg
     5401 gctgtgaata aatggcgttc aaaagcttgc atctgcggta ataagcccca tcaaacgaaa
     5461 aatatgacga gctattcttg tctggctgtt cttattaatt gaggcaggac agctttttca
     5521 caacctcccc actaaacggt gttctcctac caactcctcg gtcacaagtc acaatattca
     5581 tcaaacatga gaagctgttt ctttgaaatt gctccttgtg actcggagct gaacgacagg
     5641 ctcacgacct ctcggtaaac ggtgttcaaa agccaatttt tcgatattaa gccatttctt
     5701 tgttacttat tgtcattgat ttctttttct aatttatcca atagtggagt gtaatccact
     5761 tgagtatagt ctgttacatc aaattcatat agtgttccta actggaattt gtcatatttt
     5821 cggtcggcga tgatcttttt gaatgcttct ttcgtaatat gattggtccc tttcgttcga
     5881 tcttccaata cgtcaccata gtgataatga tccatgatat agctcaaatg acgatctgat
     5941 tcacgatcac gtttgtaccg acgttgccat aacacttcaa agtctgctgt taggcgaatc
     6001 gtgatcacct gatatccgta tctatctgct aattcttgga gtcgtgcttt ttgcttatca
     6061 ctaaaaggat actcggacac aatcactttc tttcctgctt ccatatacaa atcaagtgct
     6121 ttgtaataga aaggataaac tttcgtcctt tcttgttccg cccgttcttc caaattgtta
     6181 aacccgatgg attctgcata gtattccttg atttcatcga gcgtgatggc atacatatct
     6241 ggaaatcgtt cccgtagttt attcataaga tatgtttttc ctgtaccagg actaccagcc
     6301 agaagaatga aatacttttt catctcattt atttcctaag ttaattggac tttccaagtt
     6361 cataaacagt ccaatcttat gcttgacaat ttcttttact gctttatttg ctggaatcac
     6421 attatggcga agagacttgt tgatttctgt ttcactaaag gtttctttag ctactttcgt
     6481 ccagttgact aaaagctccg ttccaacatt gaatttgcgg atcccgttat tgatcaattc
     6541 aggataatct tcttctttca cacctgttcc tccatggata actaggggga catcaactac
     6601 tgcatcgatt tctttcagca agggaatgtt tacttctgtt tttgatttga attgtccatg
     6661 attcgtaccg atagcaatag ctaatgcatc cacgcctgta gcttctacaa atttcactgc
     6721 gtcttccggt cttgtatata ccttgtcatt ttcagcgaca tggattcctt cttctgtacc
     6781 gccgattgtt ccaagttctc cttctacgga aacaccgtgc gcatgggcat attctactac
     6841 tgcttttgtc ttcaagatat tttctttgaa aggtagatgg gacccgtcat acatgacaga
     6901 agtatatccg ctatcgattg cttctttgat atcatcaaag tctcgggcgt ggtctaagtg
     6961 taagactgca tcaaccattt cttcttctgc aatagctgtc attacattga ctaacacatg
     7021 catgccgata tatttagcgg tatcgacact tgtttgaata atgattggcg aacccatttc
     7081 ttttgctgcg cctagcatgg caggaagcat ttctaggtta tgcgtattaa aagctccaac
     7141 tgtaaaattc aactcttctg ctgtttttgt tacttctttc aatgttgtat acatctttta
     7201 ttgccacctt atctttttat ttgtttcctt tgatcgttac ttgttttgca atttgtcgca
     7261 tctcatccat tttgcccatg tagtaatcga tcccttgatc atcaccagca cgagcagcat
     7321 ctgcacgctt ctggttatag aggctcatca gttttttata cccatctccg atcgtctgtt
     7381 tattttccaa gctcttttct aaatattgga tggcttgatc gttctcatgc aattcagaaa
     7441 atttaagtcc aacttgttca taaagttccg ctaatttatg cctatcttgg gtagtggaaa
     7501 tctcttgttt caattgtgta atctctgatt gaatcttttc ggtcgcttct ggcgataggg
     7561 ctttttcttc ttttgccaga acttttttcg gttctttttc ttttttctta aaaaatccaa
     7621 acatcatttt ccctactttc aattaaaacg ctttcaaaac tgggcttaag atccaggcat
     7681 ataagagtgc tgcggctact gtatctacat cggttgctgt agcattgatg aatcccatcg
     7741 aattaaagat cgttactagc aaagcgggca ataatgtaat aaagaaacca tgagcaatac
     7801 cgccaatgat cgctcctcgg cgtccgccaa ctgcgttgcc aaatatccct gctgtccctc
     7861 cggcaaagaa attggttagc ataccaggta aaatcatcgc taagccgaac atgggcaaag
     7921 taaacatggc aataatcgta ccaatcgttg tcgtaatgaa acctaaaatg acggcatttg
     7981 ggctataagg aaagaaaact ggacaatcta aggcagggat cgcatcagga actaatttca
     8041 tagctatccc tcgaaatgcc ggcacgattt cacctaacag tagacgtaca ccagctagca
     8101 atatatacac acccactacg aattggatgg cttgtaaaaa ggcaaacatg atatagtttt
     8161 gatctcctga taattcagaa gcgaatggtt cgcctgcaaa taaaacagtg atgatatata
     8221 gcgggaccat cacgaccatg acagacaaat aagtatcttg cagaaattcg aaatgtgtcg
     8281 gcaacttgat atcttcgatc gactttttat tttcgccttt ctcaccaaaa agcttagcaa
     8341 caccggcttc aaataagtac ccgatcgtac aaaaatgacc taaagcaata tcatttgatc
     8401 cagtgatttt acgaatgatc ggttgcgcta ctgccggcat agcaatggcg aaacaagctc
     8461 cgataaaacc accgaccaag atcaatacga tccctcgcag accggcaaaa tagccaaaga
     8521 ctgtcgtcat cgttgccatc cataaaattg cttgaccagt taaaaaaatg tacttccatt
     8581 ttgtgaaacg agccaaaata atattgaata caaaaatggc taagaacgtc agtgcaatat
     8641 ctctacctaa acccaaatca ttcattgctt gaccattgat agattcaata gaagggatga
     8701 tcccttgcat atgaaatcct tcagtaaaga ttttcccgaa ataagttaaa cttccaacga
     8761 tgatgctcga accggcactc aacacttgaa atcctaggag tgtctttaac gtgccagaga
     8821 tgacttgtcc tgctgatttc ttctgcagta gtagacccaa catagcaatc aatgcaatgg
     8881 tgatcgatgc ctgagtcaaa atattttcga tgataaaatt gataacgctc atgtttttac
     8941 cctcttttct ttttgtattt agataactcc cttatcttgc agaactgggg tgattttttc
     9001 ttcaatttca ttttttgaaa caatattttt caaatagatc atcgccgttt taggatcaac
     9061 tgcgaacttt tcgaactgcg aacgaaaatt ttctgctgtg atgatcacat ctggtttcat
     9121 ggaaactgcg gaagaaatat cacaatggtc cagtttggct tctactccta atttgttcaa
     9181 aacatcctca gcagacatct gtgctgcaaa actacttcct aaacctgcgc cgcatacgaa
     9241 taaaatgttc aatttgttca tctttgttct cccccgtatc atttattatt tctcaactac
     9301 tgaaaaaagc agttgtttga aatcttcgat atttgtgcat tcaatcaatc gatccaactt
     9361 ctcttcgttg ttgatcaatt caattagttc tttcatgatg ttcagatggg agtaggagtc
     9421 tactgctgcc aaacaaaaaa taatctttac tggatccatt tcaggattac cgaaatcgat
     9481 tggttgctca atcgtagcta cgctgactcc taatttattg accccatctt ctggtcgggc
     9541 atgggctagt gccatgtgtt tgccaatcac gatatacggt ccgtaatcat tcactgcacg
     9601 aatcatcgct tcgacatagc gctcttcgac aatgttttct ttaaccaatg gtctagcagc
     9661 tagttcaatc gattcctccc aagtagtggc tttttctttg atcatgatat tgctgtccgt
     9721 caaaatatct tttaacatag gctggatctc ccttttgttg atttctaaat tgtttgtttc
     9781 gaataatttt tctaacttgc catagatgat tcgatctact cggccaccac tttctttcat
     9841 gacatctatt atttgagtga ataactccgt cgattcatga ctgttaggct ctaaccgctg
     9901 tagttcttca tttcttttca aaaagtcttc tataaccaag atattcgtct cagttaaaat
     9961 cggatcgaca actaaaagcg gcttatttcg ataattcaat tgaaaggtgc tgaaaaccag
    10021 attgacatcc aatttatcta ctaattcgat ttcttttgaa cttaaaactg cagtaatctc
    10081 gatctgagga aacttttctt tcagattctc tgctaacaga tttccagtag ccatcccatg
    10141 gttacagaca acgactgatt tataaacata attcaagtct cgcttgatta cactggcgat
    10201 cgtcgaaaag tggatgacaa gaaaagctat ctcgtcctcc agtatcttgc caccaattac
    10261 ttcttcgatc gtaggaacaa aatgagtgac tgcgcggtag attgggaaat aatttgttcg
    10321 gatgttttct ttcaatggat tcatgacttg gatatgattc ttgattctta caatcagtgc
    10381 agccatgtgc ttatagagat tttcgaataa ggcttcttcc tttagatgga aaggtacgtg
    10441 tgtctcgttc tctacaaatt ggatcaattg gatcgttagt aattgagctt gtacccactc
    10501 gattgaattg gagatatctc tagcattgaa cgtctcaata gtgaaacgaa gataatttct
    10561 ttctacttgc gggacattgt ccaattgaaa ttcctcgatc atgtggtcaa taaatgcaga
    10621 aaagctactg tcttttattt ctgtattttt ccaattaatg gcagagataa attcatgttt
    10681 cctgattcgc tggatccaga tcaatgtaaa aagcaggatt tgttttttat aaatatcatc
    10741 ttcttgtttc aacaacttat tggaatagat ctcctgtatt tttttgattt cctccaaagg
    10801 aaaataatgg agaaagattt tttgagccgg tgttaattgt gcatcttgat tcataaagaa
    10861 gtcggtcatt cccagattct gattgataaa atcatagatc atcgtacgga tagatcgttc
    10921 tgaaccttta taaaccagtc cttgctttcc ataactgacg atctctatcc cataactgac
    10981 cagtgttgct cgtaaccgtc tcatatcttc atctaatgta cttttagaga tttgaaattc
    11041 ttcttctttt ttgtttagat ggatcgggat tcggctgtaa gcaacggcta aaactagatc
    11101 aaaaatacgt tcgcgccgat tcaaaaagcc gtctttcaca tcttttattt cagtttctaa
    11161 tcgagcttgc ttttcctttt ccagcttcaa caaaaaacct ttgttgcgaa ttgtacggat
    11221 ctccggaagc tttttactgc tcaaccagat attcgcttca agaacttcct gacgaatcgt
    11281 ccgctgactg acattgaact cacttgctag atgttttgct tttactggat attgggctgt
    11341 catgattctg tgaagtattt caattgttct tgttttcata gaaatgatct ccttctgatt
    11401 tcaagtatat ggttccgctt acataataaa aagtagatat ttctgccact tgaagtggaa
    11461 agaaagtaag caaagcaaac aaaaaataag ccatatgctt tttcatctcc tcaacgaaaa
    11521 agacatacgc ctattattac aaaaattttg cccatctata caaaaaagag gttgtgaaaa
    11581 agctgtcctg catcaagtaa taagaacaac taaactaaaa tagtgcttcc tattttttgt
    11641 ttggttgttc ttattatcgc agatgcaagc ttttaaacac catttagtgg agaagctgtg
    11701 acagagatgt tcagccttga gcgataagaa gcgattttga aaaatcgctt cccatatttt
    11761 acaaaatcgt gccttattgc cgataggcta tctctggaac accgtttatt cagaaaaagg
    11821 agctgcgaca aggcttttgt cacaactccg ttcataaata tttcctgtca atccagcata
    11881 atcggttgaa taaaatcggc ttgccatctt ttttctttct cagaaaaatc aaaataccat
    11941 atctgagaaa aatactgtcg ttgtgtctta cttcctgaga tcaatcgccc atcttttcga
    12001 tcgactgaat aatcacagca tctgaaatca attcggatgg caaaactatt ttcgtggatc
    12061 tgtcgataat ttcctacttc ctgaatagtt atttttttag tatgatttct tacaccgtct
    12121 gctttgtttt tttgcagaac tttctgatgt tcggcaaaca gcttgtctgt ataacagctt
    12181 ctggcttttt caggagtttc ttcttcccaa gcggattgga tcttcatgaa tatcttttgg
    12241 atttccaaaa actgtgcttt cttttcttta gccgttcctg acatgcggga caatatctcc
    12301 gatttcagcc ttttcttttg tcttttattc ttgacgatag aaaaagcaga aacccctcct
    12361 actgacaaaa cagctaatgc aagaaacccc gtttgctcat aactagaagg acctgtactt
    12421 ggaccataat acccataaga atctctgccg taagaacgac tgccaaaata actgccccca
    12481 tatgaacgac cgccaaatga acctctgccc gaacttcgac cgctagatgt acctccacca
    12541 tgaccacccg cgacagcgaa tccttgggtc ggcagagcaa acaaacatag gcccactaac
    12601 aaaatgattg ctaaataatt gattttttca ttgtcttcct cgttatcttt tattacaaaa
    12661 aactattttt atcatagcct tgctaaaagg aagagtcaat aaccagatgc ataaactgtt
    12721 tttttagcaa ttttgtctta tttagtcatt gagtaacatc ggacagatgt atgtgtcggc
    12781 agtccataat tcctctatct tcagcatgaa caagcttgca gacaagagat ctgcgcttcc
    12841 tccaggagag atttggtttt cctgacaaaa gtgcaacagt gctgtaaatg gacgatttgg
    12901 taaagcactt tgactagcag catgagcaag tttttgcatt tccactaacg ttttctgatc
    12961 actgcgatgc aatacgcatg tgtcatctag cgaactcatc aaaactaaca acatatgcat
    13021 ttgtttctgt acatatgaat cattatctag ctccttagct gcttggattg caagcctgat
    13081 atgcggatac cctaatatcg cttcttcata tgcaccattt acgccatatt tttgcttcac
    13141 cctatgacca tgagtggttt ttttatgacg atattttgta tcaggaaaag cagcaagctt
    13201 gctgacatca tcaagtattt tcgataaaga aaaaggctgt tccatactga ttttttgtgc
    13261 aatgacactg atcaatagcc ccattgccca aatcgctcct ttatgggtat tgatgccatt
    13321 tgtagcaatc agcattgcct tttccgcttt tcgcccaatc tcagcaattc tttctcttaa
    13381 ctcctgatca atagcataat gccatgctat ttgagccatt tcttcaaaaa aaggagttag
    13441 cgtcttagca gacttatcca aaatgcgccg accttctttt acggcttttg cgatcgtatg
    13501 gaaattatca tctcctaaaa ccatatcagc tgcctgtttg cttacttcac tacctttgat
    13561 tcccatagcg attcctatat cggctctttt caaagcaggt gcatcattta cgccgtctcc
    13621 ggtcattccg acgatttctc catttttctg caaagctttc acgattcgca gtttgtgttc
    13681 tggtgtcgtt cgagcaaaga cgtcgacttt tggtacttgt cgaatcaact cttcttcaga
    13741 cataagatcc aaatcgatcc cttctaaaac tttagcagta tgttttaagc caatttgttc
    13801 cgcgatcgct tttgctgtat ctttgtgatc ccccgtaatc attttcactg agattcctgc
    13861 ttgttggcat tcctttacag ctttgactgc actttctttt ggcggatcaa tcaacccagc
    13921 aatccctgca aaagttaatt gggttaaaga ctcatgcgtg agttcctgtt tcgaatctac
    13981 ttttttgtaa gcaaatccta aaacacgctg accttttttc gcaaacgctg tggctgtttg
    14041 accccatgct tcttattctg aagaagaaag agttgattta gccaataata cttctggcgc
    14101 acctttgacg taaatcaccc ctcctttttc ctccgcatgc atcgttgcca tatatttgta
    14161 ttcagagctg aacggaattt ttgcttctat ttttttcaaa ggcagatcgg ctgtttcggt
    14221 gtggcgaatc agtgcaagtt ctgtcggatt gccacttaaa ttttctgttt tctgttcttc
    14281 tttcgtcttt aattcctgac aatttttcat aataaacagt acgcgagttt cttcctttgc
    14341 tgctacatcc acaactgtca tttcattttt tgtcaatgtc cctgtcttat ctgaacagat
    14401 cactgtcata gaacctaatg tttctacaga aggcagtcct ttgatgatcg ccttttcttt
    14461 tgccatttca tgtactccga cagataggat catggtcaac acagctggca atccttcggg
    14521 taccattgac accaccaaag cgatcatagc tgaaagaagg atatgccaat ccatgccata
    14581 gcggaatgtt gtaaaaacaa ctaagaacaa gatcaaacaa agaatgcctt gaaaaatttg
    14641 tttgttcaat cggttgatct tcttgatcag aggcgtcgtt tgttgttcga ccgattgcag
    14701 tgcttgattg attttaccga tttctgtcgt gtttcctgtt tctacaacga caccgactgc
    14761 agaaccagat tgtacaagcg ttcctgaaaa agccatattt ttttggtcac cgattgattc
    14821 ttctgctgcc aaaacggaat gatttttttc agatgcttct gattctcccg tcaaaatcga
    14881 ttcttctact agtagatcat gtacttcgat caaccgaaca tctgctggaa cgacgtctcc
    14941 ggcacgtaag acaatgacat ctccttttac tagttctttc gcagggattt tttcttgcat
    15001 cccgttcgat agtactatcg cttcttgtcc catcagctgc ttcaaaccat tcaacgattc
    15061 ttctgctttt ctttcttgcc aataactaac gagtccgttg acgatcacta cgaataaaat
    15121 aatacttcct tcgatgtatt cgctcgaagc aaatttcaaa aaagaggcaa tgatcaacac
    15181 gatcatcagc agatcagtaa agtgttttgc tatttttttc catcgttttg tctgctgtct
    15241 tgcttctatc tcattatatc cttgatcagt caggcgtttc tgccgttctt cttgcgacaa
    15301 gccttctgtt gatgtttgat agtttttcaa tacatcttct accgtcaatc tgtaccagtt
    15361 catcctatcc cctcctgttt aattgatact gttattatac gcagggaagt tttttcaggc
    15421 gtatcgatta tttcaggggg gtctgaaata attcgttttg cagaatgtat ctgggcctaa
    15481 ctgatatttc tattgcttat tcttttatat acaaaattta agaatacaaa ccagataacc
    15541 aataaaaaag gaataaagcg tgtcgtgctt tcgaataaca agctgaaagc aattgaagta
    15601 aaaaacacca acaaagcaag atctttatat ctttgtgtag tgttccgttt cttttcttta
    15661 ataaattgaa catgtgcaca caagatcgaa gaccacacaa atagaaaaca actcgttgtc
    15721 atgcctgcca aaagctgaaa aaacaatcga ctatcagcta caaaaaaatg tagacaaaga
    15781 ccgacaaaaa ataacatccc tgtaattaaa ataccgtgta aaggtacgcc tttcttagaa
    15841 agcgcacaaa accaatgggg caaacttttc tcttttgctg tttgatataa aatcctgcta
    15901 gtactgtaga ctgcactatt tgctgaggac aaagaagcaa ccaaaatcat tagattgaca
    15961 attgctgctc cgctttggca atggatcact tgcagcatag tcactaaagg actttctttt
    16021 aaatctaaac aatgccatgg aacaacaaat aaaatcgaca aaatcgttcc tatatagaaa
    16081 aagaggatac gccaaggcag acagttgatt gcatttttca aattcttttg tgggtcactc
    16141 gtctcaccaa ctgttaatcc gatcatttca attccagcaa aactgaataa aaccatagga
    16201 aatgaagcaa tcattccagc aaagccaaac gggactaatt cattgagctg tccaaaattt
    16261 gctttgattc caatagactg ggtatgaaaa tctgagacac tcaatcctat acccaataag
    16321 ataaaaagga aaatccccaa gatcttgatg attgagaaaa aagattccaa ttcaccaaac
    16381 cacttgactg caacaaggtt gactgccatt aagatcatta gtgcgctacc tccgcttatc
    16441 aaagctggta catgtacaaa ccagaaatgg atatacaatc ctattgctgt taattccgca
    16501 agtccagctc caagccagca aagccaataa gtccaattga caaaaaatgc ccactttttg
    16561 cctaaataat agtcaacgaa atcactgata gagttaaatg tactatttgc taaaaataaa
    16621 tcacccatga cccgcatcaa accataaata gccaaaccgc atatcaaata aacgagaata
    16681 acagctggcc ctgctgatgc aattgccttt gctgaaccta aaaataatcc tgttccaatt
    16741 gcgccaccta atgcgattaa ctggatattt cgattagtta gagtccgttt cattgccggt
    16801 ttttccatca agttcacctt tttctttttc aagcagcatg gcgacttcct gttcccaaga
    16861 aggtttcttt attttttcaa tacagagtgg tactacaaac aaaatgacac tgatggctgt
    16921 gactgcaatt agattgatcc atgtcccagc tttcatcgtc agtccgacag aaaatccgat
    16981 tgccgcaaat aaaacaataa cgacagtcca aactccaaga ttgctctttt tctctttttt
    17041 tccaattcta aatggtcgat tcaactttgc atgtgtataa cgcaatctca aaactgcgat
    17101 ccccatgatg acatagacga tacagtagat aacagtagtt gaatttacga gcatcaaaaa
    17161 tgcttgattg acgccaggaa tcagcaaata aaaaagtgca aagattgaga taatgacaga
    17221 ctgagtcaaa ataacggatt tggatacgtc aaattgattc gtcttccaaa aattgaattt
    17281 tggtggatac gcaccttttc tagcactttc tgtcactgtc tttgctggtc ctgaggccca
    17341 agcagataat tggactgcta caccaatgaa gacaagtaaa ctaaacagat tcacaataag
    17401 atgtggcaaa ccaagaattt ggcaataaat cgaaatagat tgcgcgatat tattcaattc
    17461 catttgtcct tttggtacga catttgctac aactaaagca ttcaaaatat tgacgaaaaa
    17521 tataaaaatt aacgcggcaa atactccgcg aatatacgtt ttaactggat ttttcaaccg
    17581 agtgatatac acagaggaca tttcgatacc agtaaaaata aacatgatcg gcgcaaaata
    17641 aacaaatgag gatgcagtcg tagtatctgg tattaatttg tcccatgaaa aactgcctaa
    17701 tgttccggtt ggtacgatcc caactttgat ccacgcagca aaccctaata agagcatcat
    17761 cgtcaaaggc acgtacaatc ctaaccaaac accgactttt ccgccgattt ttgccatatc
    17821 aaatttcaaa ttcaaaaaag tgatgatcca ataaacgact aaaatgacaa ctaatgtaaa
    17881 tttgctgttc aaaccaagcg gcacattatc gataatatta ccaaacaacg gagcaagtgc
    17941 tgaagcgacc atcaccatcc caggaaacat ttggacccat aacagccatg agacaacgaa
    18001 cccccatttt tttcctagag cattcgatac ccaaagttct ggaccacctt tttggggaaa
    18061 cattccggca aattcaccag agatcaatga gattggaaaa gcgtacaata atactgcaac
    18121 cagcatgtaa aaaatcatcg tccagctcgt tgctgctaca tctggaatat ttcgtacact
    18181 tgctacaagg gcgcaggtca ttccgataaa ggtcatggtc gtgatttgct ttgatgaacc
    18241 agtatctttc atctttattc cttctttcaa tagcgacata tgtttttgtt ttcttttatt
    18301 tttgttcaag aacagaaatg acaaggtttc atcgattcag tacaaaaccc agtatcaccg
    18361 aaacatccat tgaacgatct tagtgaactg ggtttgatac atgaaacgaa cgatccaact
    18421 tatagaacgt tttataaaaa agaatatctt ggcgaggaat atatgaaaaa cagggggaaa
    18481 tacaattgtt ttgatgaagc aaataattgt ctgttctcca tatgtctctt gttattcttt
    18541 cacacaaaaa tcagttctat tggatgattt cgtttagttt cttttccatt gctttggcga
    18601 tttcttttgt ataaaaattg aaaattcgat catcattcaa ataaggagtc atgatagccg
    18661 cacgtagcag agtgattttc tgttcttttt tccattcttc tttcgttaga cccattcttt
    18721 caacaaaacg aatcggagaa tcaccgtaat cttcttgtgt aaatgtagta tgagaagtaa
    18781 taaaccgttc accataaaca tcgccatcca tataagaaga ccgatcgaac attttttcat
    18841 ttaattcatt gatagcgttc aaatctgtac agccttgttc tttgaatacc caattgacca
    18901 tattaaaatc aggatgattc aatggataga cttcgattgt ttttccttta acagtaaaag
    18961 tcagatgatc gagaaattct ctgaatcttt gagctgcctc gattgaagcc ccaatcaatt
    19021 gaccatatcc ggtcacattc aatggcaata cacgatgtgc tgtccagact gcggcggcgg
    19081 ttgctccagc ttttgatcct tccaaaatat aagctcctaa catatctgga gcctttactg
    19141 atttttcaaa cacatatggc gcgaaataag aaatgatatt tctcatattt ttatgtttga
    19201 ttacgattcc tccagcagca taagggacat agcccatctt atgaggatct atcgtgacag
    19261 aatccgcttc tgaaatagct ctgaaccctt catatacctc tttgtcaatc gttacgcagt
    19321 ggtggaaaac atgatgttct tcaaagaatt cagctaagga agcatacggc acaaattctc
    19381 ctgcttcgtt tagaaacagt gaacgcgcat agccgccata tgcggcatca acatgtaaat
    19441 agaaatagat cccttcatct tttaaccttt ctcttaattg gacgatttta tctacactgt
    19501 ctacttgacc ttcttctgtc gttcctacga cagcaaccac acctagaatt gggatttttt
    19561 gttcagctaa ttcacgaatt gttttttcca aagcattaat atccattcga taatcctctt
    19621 gaactgggat agcaaccatc tgatctaagc ctactccgca aatatctaac gccttcatcc
    19681 aagaatagtg ttttgtttga ggcaccagcc atttccctaa tctttggata ttttttccgc
    19741 tacgggaaga agctgctttg acctcatcca tttcttcgtc cgtaaagctt tcggtcattt
    19801 ccaaaatttc ttcaacagat aaattcaaaa gttcccactc agacattttt ttcacttgtt
    19861 caggcagcac ttccttgaca gctaaaggaa tcgatttgat gcagcgggcg taccataatc
    19921 cttcaagatt agcaatagaa ccatctgctg caatgtgtcc ccatccgtct gtcatattga
    19981 ataatgaagc aaaatcctgt cctacttctg cttccatttg cgaagttgcc atagaagatt
    20041 ccaatgcaac attatttggg ttccacagca tcgcatagtt gtaagctaat aaagcaggca
    20101 tcaatgtttc agcattcatt tgtccccagt aacggcctgc tgaatgccat gggatcgatc
    20161 ctgcgcgtaa tcgctgactg acttcatcca agacctctat catatggtcg cgggtggcta
    20221 gataatctgg agtgagctta tctccttcgc taattgcagg catatctgaa ggaatataat
    20281 tttctctcca tccaagatgt tcatctacca tcttgttcaa caacatttta taaactggtc
    20341 cattttcagc tttgtcgcct ataaagacgg ccttgatatc catatctttc atttttcttc
    20401 cctccatatt ttgttggtca atgtctcgca gatacatgtg tttatctgta acacctcttc
    20461 actatagcgc caaaaatgag aaattttttt ttataacatc aaatccacgt agatttttac
    20521 a
//
LOCUS       DASHRQ010000050        20354 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.053, whole genome shotgun sequence.
ACCESSION   DASHRQ010000050 DASHRQ010000000
VERSION     DASHRQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.053"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            286..1425
                     /locus_tag="U2M88_001952"
     CDS             286..1425
                     /locus_tag="U2M88_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEM8374317.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1492..2067)
                     /locus_tag="U2M88_001953"
     CDS             complement(1492..2067)
                     /locus_tag="U2M88_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEM8374318.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2072..3046)
                     /locus_tag="U2M88_001954"
     CDS             complement(2072..3046)
                     /locus_tag="U2M88_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEM8374319.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3070..3114)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3329..4297
                     /locus_tag="U2M88_001955"
     CDS             3329..4297
                     /locus_tag="U2M88_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM8374320.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4410..4688
                     /locus_tag="U2M88_001956"
     CDS             4410..4688
                     /locus_tag="U2M88_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEM8374321.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4806..5042
                     /gene="secG"
                     /locus_tag="U2M88_001957"
     CDS             4806..5042
                     /gene="secG"
                     /locus_tag="U2M88_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEM8374322.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5145..5900
                     /locus_tag="U2M88_001958"
     CDS             5145..5900
                     /locus_tag="U2M88_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM8374323.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5903..8260
                     /gene="rnr"
                     /locus_tag="U2M88_001959"
     CDS             5903..8260
                     /gene="rnr"
                     /locus_tag="U2M88_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEM8374324.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8282..8746
                     /gene="smpB"
                     /locus_tag="U2M88_001960"
     CDS             8282..8746
                     /gene="smpB"
                     /locus_tag="U2M88_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEM8374325.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9048..9767
                     /gene="atpB"
                     /locus_tag="U2M88_001961"
     CDS             9048..9767
                     /gene="atpB"
                     /locus_tag="U2M88_001961"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEM8374326.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9820..10035
                     /gene="atpE"
                     /locus_tag="U2M88_001962"
     CDS             9820..10035
                     /gene="atpE"
                     /locus_tag="U2M88_001962"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEM8374327.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10126..10650
                     /gene="atpF"
                     /locus_tag="U2M88_001963"
     CDS             10126..10650
                     /gene="atpF"
                     /locus_tag="U2M88_001963"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEM8374328.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10637..11179
                     /locus_tag="U2M88_001964"
     CDS             10637..11179
                     /locus_tag="U2M88_001964"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEM8374329.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11202..12758
                     /locus_tag="U2M88_001965"
     CDS             11202..12758
                     /locus_tag="U2M88_001965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEM8374330.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12772..13674
                     /locus_tag="U2M88_001966"
     CDS             12772..13674
                     /locus_tag="U2M88_001966"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEM8374331.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13697..15103
                     /gene="atpD"
                     /locus_tag="U2M88_001967"
     CDS             13697..15103
                     /gene="atpD"
                     /locus_tag="U2M88_001967"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEM8374332.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15117..15539
                     /locus_tag="U2M88_001968"
     CDS             15117..15539
                     /locus_tag="U2M88_001968"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEM8374333.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16008..16238
                     /locus_tag="U2M88_001969"
     CDS             16008..16238
                     /locus_tag="U2M88_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEM8374334.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16335..17636
                     /gene="murA"
                     /locus_tag="U2M88_001970"
     CDS             16335..17636
                     /gene="murA"
                     /locus_tag="U2M88_001970"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEM8374335.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17649..17825
                     /locus_tag="U2M88_001971"
     CDS             17649..17825
                     /locus_tag="U2M88_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEM8374336.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17960..18256
                     /gene="yidD"
                     /locus_tag="U2M88_001972"
     CDS             17960..18256
                     /gene="yidD"
                     /locus_tag="U2M88_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEM8374337.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18320..18703
                     /locus_tag="U2M88_001973"
     CDS             18320..18703
                     /locus_tag="U2M88_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8374338.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18872..19756
                     /locus_tag="U2M88_001974"
     CDS             18872..19756
                     /locus_tag="U2M88_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM8374339.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19870..20106
                     /locus_tag="U2M88_001975"
     CDS             19870..20106
                     /locus_tag="U2M88_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEM8374340.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20194..20274
                     /locus_tag="U2M88_001976"
     tRNA            20194..20274
                     /locus_tag="U2M88_001976"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20228..20230,aa:Tyr,seq:gta)
     gene            20281..20352
                     /locus_tag="U2M88_001977"
     tRNA            20281..20352
                     /locus_tag="U2M88_001977"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20313..20315,aa:Gln,seq:ttg)
BASE COUNT         6759 a         3566 c         4425 g         5604 t
ORIGIN      
        1 tgcaactagc ttctgtatat gatgtttaca aagaatggta caagaaaaga tcatccttga
       61 aaaaggaaag ctaacaataa ctagcatcat ataagagctt ttaaggatag aacagtagaa
      121 taggaatcta tgggcaagtc ctctcgtata aataaaatag gagggaagta gcagcatatt
      181 tttcataaag tggctctctt tcttcatttt tactttggtt gctcctattt gaaaattcga
      241 gcacactcaa atacaataaa aagaaatggc aagggagagg attagatgaa aaaagaagaa
      301 aaaatcgcga ttttacaaga gattatccga attaaatcag tgaatggaaa cgaaggtgaa
      361 gtagcagcct atttgaataa gcttttggca aggcatgaca taacaggaga gattgtttct
      421 tacagagatg gtagagataa tttgattgct cgttaccaaa agggccaatc aggaaaagtg
      481 ttaggcctat ctggacatat ggatgtcgtg gcggctggtg atgaatcgtc ttggacctat
      541 gcaccctttg cagcagaaat ccatgggaac cgtttgtacg gacgtggtgc tacagatatg
      601 aagagtggct tggcagcaat ggtgattgcc atgatcgaac tgaaagagtc aggaaaacca
      661 tttaacggaa cagtcaaatt attagccact gtcggagaag aagtgggtga actaggtggg
      721 gaacagctca cgaaagcagg ctatgtagat gacttagatg ccttgatcat cggagagcca
      781 accaattaca gtttgatgta tacgcacatg ggatcaatca actatacagt cacctcgcac
      841 ggaaaagaag cgcatagctc tatgccagac caaggttata atgcaatcaa tcacctaaat
      901 gaattcatta cgaaagcaaa tgctgaaatg aatcacctcg ctgaaacaat cgagaatcct
      961 gtcctaggaa aaacaatcca taatgttaca ttgatcagtg gcgggaatca agtcaacagc
     1021 atcccaagcc atgcgcagct ccaagggaac attcgatcga ttccagaata cccaaatgat
     1081 aaaatcattg cattgctgca atcaatcgtc aatgaattga atcaagagac agactatcat
     1141 ttggaattga tgatcgatta caacaaaata cctgtcaaag ctgatccaga ttctcctttg
     1201 attcattcca ttcagcaaca attcagccag ccattacctt tagttggagc ggcagcaaca
     1261 acggatgcag ccgaatttac gaaagctaac cattccttcg actttgtcgt ctttgggcct
     1321 ggtgtcgtta ctttgcctca tcaagtagat gagtatgttg aaatcgataa ttatttggat
     1381 atgatcgaaa aatatcaagg aattatttta tcctacttag cgtaagattt ttttaataaa
     1441 tgaaaaaaca gagccagatt agcattattt tgctaatcca gctctgttta ttcagcctat
     1501 ttttcttttt gaatatatca agcgttgggg caacatagtg atgactaatg aaacaacgac
     1561 gatagacaac aagcgatcaa ggtagtctgt acccacctgt accaagatca cacttgttgt
     1621 ctgacttaaa cctaacccat gtaatagctg aacgatcata ctggatcctg aggaggtgat
     1681 cccgccaaat aactgaacgg taatgatcga agaaacgatc gttccaggta ccgacaaaat
     1741 caaagcgagt aataacagtt tcaagctgtt tttatctttg ccgagatatc ttggcaaaat
     1801 caatccagca agcaagcctg tgagaatttg gattggcgaa taatacagag aaaaaatatc
     1861 tgtagtcact ccacttaaaa taccgctgga caatcctgtc agcattccag gtacaggccc
     1921 aagcaatgca ccagcaaaaa tcgttccgat agaatcgaga tagattggca aacgaagcca
     1981 taaagcaatc gttcctccaa tataattgat cccaatacac aaagccatca ctgtaagtac
     2041 gcgagttgcc attttgtttt tattcatttt gttagcccat ctttaacctt tccaaatctt
     2101 tcataatcgt ttcttcttgt gcatttaaaa gaacggttaa aaactttcgg aaaaaaagac
     2161 gagtatctac ttttgtcata attttactgt tagcaggttt ctgccaaaat tcataacgat
     2221 caaccaatgt ttgtccgcga cttattcctt gagtttctac agcagtgaag cttttaaagc
     2281 cttcacagat ggactcatcg ataaaataag caacagctaa tggatcattg atgacacatc
     2341 ctaagattct ttcttgtttc cagtgaaagt caaaataaaa acgagtgatc gcttttagat
     2401 actctccctc ttcaggattc atttggcagc aatattcaag gatagtcggt gtcaagacga
     2461 tttctcttgt cacatctaac ccaaccattt ctatcggttg tttgagcttt tcgaatacat
     2521 aagaagctgc ttcaggatca caccagtaat tgtattcagc aactggtgaa cagttaccat
     2581 ggcttttgta agttccgccc attgagacaa aacgatccat atgtttccca acagatgggc
     2641 tgtttttcaa agcagaagcg atattggtca aaggccccaa agcaatgact gatgtatctg
     2701 ttttttcttt gaaataatca gtcagaaaat caactgcatg gagcgattct gcctgtttgg
     2761 ctaagaccat cgggaaattt gtgtccccta atccatccaa cccatgagtg tcttgcgcac
     2821 taatgaaagg ttgcttcaaa ggtttttcca ttccttgata aacagggata tctaaacgat
     2881 cacagcgctc caaacatttc agcacattct gcgtaccgat atctacaggg acattcccgc
     2941 aaacgacggt aatagcaact acttcgatat caggggattt gacagctagt gataaagcta
     3001 aagtgtcgtc gatccctgga tcgcaatcaa taattacttt acgcattatt tccaatctcc
     3061 tttctttaga gatacttagt tttttattct gggaagtttc cgaaccagtc caagacgagt
     3121 gtcttgttta taatgcacga aattagtata ctggctttta ttgtgaatgt aaatgctctc
     3181 ttacaagtaa atctattgag aagaacttta agtaaatgat acaatgaaaa agaaatggaa
     3241 tttacgtgtg ccgaagaaaa agaatcctat tgatcggtat attgtataat aaatagatta
     3301 tagtttggca gaaagcggcg ggtgtacgat ggttattttt tatttcattc ttattctgat
     3361 atttatagga atcagttacc attttctttc ttatttttat gcaaagaaat tagtaagagc
     3421 gggggtacag agaggctatg actggtatga agaaactggg caccgcttga tggatcctgc
     3481 tgcagtcacg tcagtagaaa aaaagcgaca aaaattagaa gaagagtgtg aaagcttctg
     3541 ggaacaagga ataccgaaaa cgatcaaaag ttatgacggg ttgaaactag ctggacagat
     3601 gttcctccaa ccaactcagc agaataaatg ggtcatttgt gtacatgatt atcgaagtac
     3661 agggaaaaga gatatgtctc atataggtaa aagatatgca gagaaaggat tcaacgttct
     3721 tatacccgat ttacgtgcgc atggagaaag tgaaggagaa atcatcggaa tgggatggct
     3781 agaccggcta gatttaattg catggatcca actcatctta gatgaacaac cagatgcatc
     3841 cattattttg cacggcagtt cgatgggcgc ttctactatt atgatggcaa gtggtgaaaa
     3901 gcttccatct gcagtcaaag gatttatctt agacagcgga tatgtttctg tttatgcgga
     3961 atttcggtat atgttaagta aaatcacggt ctttcctaaa aaaatgatca tgagatatgc
     4021 gaatcactat gctcagaaat acgcaggtta ttctttaaag caagcatcag ctacaagaca
     4081 gcttggtagt aaccatttgc ctttgttagt catccatggt gaaagagatc attttgttcc
     4141 tacagaagca gcatacacga ttcaaaacgc tacggcaggg gataaagcat tactgcttgt
     4201 tccagaagca gaacatttgg aagcatcgat gaaagatccg aaaacctatt ggacagtgat
     4261 tttttcattt attgaacaaa gaataacgat ggattgattt tctcttcctt tttgtaagca
     4321 acacgaggat aaataattgt ttatagacca tgagactgtt aaaataaggt aggaataaaa
     4381 aaatatcagt tatttgagga gtgcggcata tggaaatcaa agaggaaaaa aatcgtcttg
     4441 ctttattcaa tgatgagcaa caagaaatcg gagaaatgac atggtcggat gcggggccag
     4501 acatcatgat cattgaccat acatttgtcg atccagcata tcgtggtcaa aaattagctg
     4561 aaaagctagt gtatactgga gtagaactag caagacgaga ggggaaaaag attatccctt
     4621 catgccctta cgcaaaaaaa gagtttgaga agaaaccaga gtatcatgac gtcttacgcc
     4681 aatcataata ataacagctg aaagatttgc actttcagct gtttttccat ttatatagag
     4741 ttgtcacgga gttttatcta tgataaagtt aaagagacga tatcttttgg gaaggaggct
     4801 gctatatgta taatttaatt ttgggaatcg tgatcgtttt atcgattgtt atggtcattg
     4861 cgattatgat gcagccaagc aagcaaaata gtgcagcgag tgcatttacc ggcggtgcgg
     4921 atcaactatt cggcaaacaa aaagctcgcg gctttgaagc tgtcatgcag cgatcaactg
     4981 cagtcatggg agctgtctgg atgatcttat tatttgtcct agcattttta tcttcgaagt
     5041 agtcgaataa gcctctcgca caacggcggg cggctttttt tattgcaaag agaacgctta
     5101 agaagaagtt atgaaagaaa ctatggtaaa atgaggcgaa agaggtgaaa cgagtgaaac
     5161 aattaccaaa acccctttat gcaaaacatg gaaaacgagc agttttgctg cttcatgcgt
     5221 actcaggaag tccaaatgat gtccggatgc tcgcacggtt tttagaaaaa gcagattata
     5281 cagtctatgc gccgctattt aaaggacatg gaacgatgga gccatatgat atcttgcaag
     5341 aaaaagcaga aagctggtgg gcagatacta aaaaagctat ccatttttta cagtctgaac
     5401 agttttctga tattgccgta ttgggattat cgatgggtgg catctttgct gtacgggcac
     5461 tagaagaaga atcagtaatt ggcggcggtt ttttctgttc gcccctgtct cctgtagaaa
     5521 caaacgtgcc tgaaaatttt gaaaaatatg tacgtcaagt gctgaagata gcaggcaaat
     5581 cagaaaaaga aatcaatgaa aaagctgtgt cttaccgctc gttagctgaa catcaattaa
     5641 tggatataca agaccaagca gcaatcgcag aaagtagact atcagatatc caacagccaa
     5701 tatttttagc tcaagctggg aaagatgaaa tgatcgaccc aaatggtgta tttgaaacgg
     5761 cacgcaaatt gagccgccaa cgagtgacac ttcaatggta tcctgaaagc ggtcatgtga
     5821 ttacagttgg cacagcaaga cgcgaattag aaaaagacgt gttggaattt ttagaaaaat
     5881 taccttggaa tgaggaataa atatgacaaa agaaacaata aaagagaaaa ttctcttttt
     5941 catggaaagc agtcgcaaac gtagtttttc tatggaagaa atcgctgaag gtctaggctt
     6001 tcaaaaaagc gatgatttta aattactagt acaaacgatt gctcaaatgg aaagagaaca
     6061 gagtatcgtt ttcaataaaa aaggaaaagt caaacttcct ttaaaaccga tcttggtcga
     6121 agggacattt cgggcaaatg aacgaggatt tggatttgta acgattgatc ccgaagaaga
     6181 tgatatctat atcccgaaag aagcgactgg ctttgcaatg gatggggata ccgtagcaat
     6241 tgatatcatg aagcgagcaa atacagatat ggatcgcgga gcagagggaa aagtagtgga
     6301 gattcgagac cgagcgacta ctcagttagc cggagaattt gttgcttatt cagacgaaga
     6361 gatacaggaa acggatcttt atggtgtggt catcccgaaa gatagaaagc ttaaccaatt
     6421 caaagtgtat gcagcggcag agggagttcg tccagttgac ggaagtattg tattgatcga
     6481 actgacccat taccctgaaa aagggtatgc aactagttta gaagggataa tcaagcaagt
     6541 gattggtcat aagaatgacc ctggcatgga tatcctctcg attgtcgtgg ctaacggtat
     6601 tcctacaaaa tttcctgatg atgtgttatc tgaggcagat gaagttccag accagatcag
     6661 cgaaaaggat attgttggac gcagagattt aagagatcag ctcatcgtta cgattgatgg
     6721 agaagatgca aaagatctag atgatgcagt gacggtcaaa aaattagaaa atggtaatta
     6781 ttttcttgga gtacacattg cagatgtatc ttactatgtg acgcaaggca gtcaactgga
     6841 cagagaagca tacgaaagag gaacaagtgt ctatttgacc gatcgagtga ttccgatgat
     6901 cccacaacgt ttatccaatg gtatctgttc attgaatccc catgttccac gactcaccat
     6961 gagttgtgaa atggagatcg atccaaatgg acaagttgtc tctcatgaga tttttccaag
     7021 tgtgatccaa acaacagaac ggatgaccta cacagctgtc aatcaaatct tagaagatca
     7081 agatgaagaa acgatgcaac gttatgctca gctcgtgcct atgttccaag aaatgggtga
     7141 attgcatcga atccttgaaa tgatgcgaat caaacgtgga gcgatttctt ttgaggatcg
     7201 tgaagcgaag atccttgttg atgctgaagg acacccaaat gatatccagc taagaagcag
     7261 agggataggt gaacgcttga tcgaatcctt tatgcttgct gccaacgaaa cggtagctga
     7321 acattataat aaattaaatc ttccgtttat ttatcgaatc catgaacagc caaaagaaga
     7381 aaaaatgcag cgcttctttg attttgcttc agctttgggc attctggtcc gaggaacgaa
     7441 aggaacgatc acaccaaaag atttacagca tgtgatcgaa ggagtagaag acaaaccaga
     7501 agctgcagtt atcaatacta tgttgctaag aagcatgcaa caagcacgct attcagaaga
     7561 caattttggg cattatggct tagcggcaga ttactacaca cactttacat ctcctattcg
     7621 acgctatccc gatctaatcg tccatcgtct gatccgttct tacagtgaag ataaaagtga
     7681 aagcaatcaa agttcgtggg ctgcggaact tcctgaaatt gcagatcata gttctaagat
     7741 ggaacgtcgt gcagtagaaa cagaacgtga agtagattcc atgaagaaag cagaattcat
     7801 gatggacaaa gtgggagaag aattcgatgg gatcatcagc tctgttgtga aattcggcct
     7861 gtttatcgaa ctgccgaata cagtagaagg attgatccat atcaatgaat tgaaacagga
     7921 ctacttccat tttgttgaaa atcatttagc tcttgtaggt gaaagaactg gtctgacttt
     7981 taaaatcgga caaaaagtgc gcgtaaaagt agtcaaagct gatccagatg aacgagcagt
     8041 ggattttgaa ctcgtttccg cagaagaggt cactgcgttg gaacgtccaa aagcacgcaa
     8101 tggaaaatct ggaaaacgag aaggaaaaca agcaggcaaa cgctcagaaa aaaatggcaa
     8161 gaagaacaaa cgatatagtg atagtaagca aacgttcggt aagaaaaaag ggaaaaagcc
     8221 attttacaaa ggtgtcaaaa agaagaaaaa gaaaaaataa aatgagttgg aggaaacagc
     8281 tatgccaaaa ggcgagggaa aactaattgc acaaaacaag aaagctcgcc atgattattc
     8341 gatcatcgac acgatggaag cagggatggt cttgcaagga acggagatca agtcgatacg
     8401 aaacagccgg atcaatctga aagatggatt tattcgcgtc cgcaacgggg aagctttctt
     8461 gcataatgtt catatcagtc cttatgaaca aggaaatatt tttaatcatg atccgttgcg
     8521 cacgagaaag ttattattac acaagaaaca aatcgttcgt cttgaaaacg aattgaaaaa
     8581 cactggaatc actgttgttc ctttaaaagt ctatattcgt aacggctatg ccaaggtatt
     8641 gattggtctg gcaaaaggga aaaaatcgta tgataaacgg gaagatttga aacgaaaaga
     8701 tatcgatcga caaattgatc gaacattaaa aaatttctct agataatttc attatccttt
     8761 ttcgcatatt cggtgctttt gcttttactt aatgataaaa tttgttagca tttaatctga
     8821 taaaacatga ttatcacagg ttttttctta gatcaggagc accgtgcatg gggaaaaatt
     8881 cgcttcgttt ttgactgaaa taaaaaagtg tctaattttc atgaaaatgt tttgaatttt
     8941 ctgtttcatg gtggtatgat accgtgtggt tagaaaccga ttggaaaaaa ccagatacgc
     9001 acatgaattg attttatgtg aatttcgggg aaaagaggtg aaatggattg gatgaacgct
     9061 cgctaacgtt tcacattgga ccaatttggt ttgatggtac agtttgtata atggtgttac
     9121 tgacctgttt gattgttttc ttcttagctt acttttttac aagaaatctt aagttgaaac
     9181 caacaggcaa acaaaatgcg atagaatggg tcattgattt tagtcgagga atcgtaacag
     9241 ataatctgcc tagaaaagaa ttgagcaatt tccatttgtt ggcatttaca ttgtttttat
     9301 ttgtttttgt tgcaaacaac ataggtttag taacaaaaat cgtcttacct aacgaaacaa
     9361 cactttggaa aagcccgaca gcagacccgt ttgttacttt aactttggca tttataatga
     9421 tcttacttac tcacttattc ggggtgaaaa agttaggctt caaaggatat ttcgtaaatt
     9481 cgttcttgaa accctatagt tttatgtttc cgatgaaact gattgaagaa tttacaaact
     9541 tattaacact tgctttacgt ctttatggga atatttatgc gggggaagtt ttgctaacct
     9601 tgattgcaaa aatgatgaca agcttaggct ggttctcatt accattagca attccattag
     9661 agatggtctg gatcgcgttc tccttgttta ttgggagcat ccaagcattt gtctttgtca
     9721 ctttgtcaat ggtttatatg agccataaga ttgaagtaga agaataatac tcggttaaaa
     9781 aacaaactac tatttatcta ttttaggagg aaaacaatta tgaattatat cgcagcagca
     9841 atcgcaatca tgggagcagc aatcggagcc ggttatggta acggtcaagt tatctctaaa
     9901 acaatcgaat caatggctcg ccaacctgaa atgtctggtc aattccgtac aacaatgttt
     9961 atcggggtag ctctagttga agctgttcct atcttagggg tagttatcgc cttgattcta
    10021 gtttttgccg tttaattgaa tcagtattaa ggcagaaaca acatcgtttg ttactgcttt
    10081 tttacatgga aactagaaat actccaaaga aaggaaggct cgcctatgct gaatcaattg
    10141 gcaattgcag aagttggcaa tgcgatgtta ggtaatatca tcgttgtcag cggctcattt
    10201 ttgatactac tggctctctt gaagcacttt gcttggggac caatcagcga tattttgaaa
    10261 aaacgtgaag acaagatcgc caatgattta gattctgcag aacaatctcg catcaactca
    10321 gcgaaaatgg aacaagaacg cgaacaacaa ttgttagcct ctcgttctga tgcagctgat
    10381 atcatcaaaa atgcgaaaga aagtggagaa ttaagccgcc aaaatatttt gaaggatgct
    10441 caagaagaag cagctcgtct aaaaagcaaa gcccaagctg atatcactgt agaacgtgat
    10501 tcagcgctga actctgtaaa agacgacgtt gcagaactct ctcttcaaat cgcggaaaaa
    10561 atcttgaaca aagaattatc tccagaaatg catgaatcat taatcaatca atacattgaa
    10621 ggtctaggtt cttcaaatga aactagataa atatacagta ggaagacgtt acgggaaagc
    10681 tttatttgaa ctggcgatcg attcaaatca agcagaagaa atctatcaag atctcgtaag
    10741 tttacgtcaa atctatgaac aagtacctgg attagggaat atgttaagtg atgttcgtct
    10801 agaacctcat gaaaaacgag aaatcatgga caagcttgtc agcggttatg atggaatcgt
    10861 taaaaacttt ttagaagttg tttatgagta taaccgaatg gatgaccttt tgttcatgat
    10921 tgaagaatat gaacgtcgat atgatgaaca tcaagggtta ctattgggaa gtgtcacaac
    10981 agcagtccca ctttcagacg aacaacgaat acaattagaa aagaatgttg caaaaacaat
    11041 ggactatcaa tcagttgaat taaaacaaat cgttgattct tctattattg gtggcgcgat
    11101 tgttgaagcc aaccatcgag taatcgacgg aagcattcgc acgcaattag aaaaaatgcg
    11161 taatcaatta aacagatagt acaaagaggt aggtgaatcg aatggccatc aaagcagaag
    11221 aaattagtgc cttgattaaa gaacaaattg aaaattatca aaacgtgctt tcagtcgaag
    11281 aaatcggtac cgtaacttat gtaggtgacg ggatcgctcg tgcacatggt ttagaaaacg
    11341 cgatgagtgg tgagttgctt gaattttcaa acggctcata cggaatggcg caaaacttag
    11401 agtcaaatga tgtaggtatc attatccttg gcgatttcga gtccattcgt gaaggagata
    11461 aagtaaaacg aacaggtaag atcatggaag ttccagttgg agaggccttg atcggtcggg
    11521 tagtcaatcc gctaggtcaa ccaatcgacg gactaggtga aatcgttact gacaaagctc
    11581 gtccagtaga agcgatggca ccaggtgtta tgcaacgtaa atctgttaac gaaccaatgc
    11641 aaacagggct aaaagcgatc gacgcccttg tgccaatcgg acgcggacaa cgtgaattag
    11701 tcatcggtga ccgtaaaaca gggaaaactt ctattgcgat tgatacgatc atcaaccaaa
    11761 aaggtcaaga tatgatctgt atctatgtag caatcggaca aaaagattct acagttcgta
    11821 cacaagttga aacattgaaa aaatatggcg caatggatta cacaatcgtt gtgaatgccg
    11881 gtgcgtctca accagcacca ttactttata tcgcaccata tgctggtact gcaatgggtg
    11941 aagaattcat gtacaacggt aaacatgtat tgatcatttt tgatgatctt tcaaaacaag
    12001 ctgttgctta tcgtgaactt tccttgctac ttcgccgtcc accaggtcgt gaagcttatc
    12061 ctggggatgt tttctacttg cactcacgtt tattagaacg tgcagcaaaa ttaagtgatg
    12121 atctaggcgg cggttcaatg actgctttgc catttgttga aacacaagct ggagatatct
    12181 ctgcttatat cccaacaaac gttatctcaa tcactgacgg acaaatcttc ttagaaagcg
    12241 acttgttcta cgcgggtact cgtccagcgg ttgatgccgg actatctgtc tctcgtgtag
    12301 ggggttctgc gcaaatcaaa gcaatgaaaa aagttgctgg gactttacgt ctagacttag
    12361 caagttatcg tgaattagaa gcatttacac aattcggttc tgatttggat gcggcaacac
    12421 aagcaaaact aaatcgtgga cgccgtacag tagaaatctt gaaacaaaaa ctgcatgcac
    12481 cgctagctgt tgagaaacaa gtagtgattt tgtatgcctt gacacatgga ttcttggaca
    12541 gtatccctgt agacagtatt ttagacttcg aacatgaatt attcgaatat ctggatacca
    12601 atcattcaga tatttttgaa acaatccgta caacaaaaga tcttccagaa gaagaaacgt
    12661 tgaacagtgc gattcaggaa tataaagaca tgttcttagc tacaaaagga aataattcct
    12721 ctacagaaga taaattgaaa tccattcaaa atgcatagtg aggtgagatg aatgggtgct
    12781 tcattaaatg aaatcaagac acgtatcgct tcaacgaaaa aaacgagcca aatcacgcgg
    12841 gcaatgcaga tggtgtctgc ttctaagttg acaaaatctg aagcttcttc acagaagttt
    12901 caggtttatg caacaaaagt aagagaaatc gttacgcatt tgactgctac tcagttaaat
    12961 gatattgcct cagaaaatcc tagaggggat atcaattaca acagcatgct gctcagccgt
    13021 cctgtgaaga aaaccggtta tatcgtcatc actgcagatg gcggtctggt cggtggatat
    13081 aacagttcga tattgaaaca aacgatgtct attttagaag aagaccacca atcacccgat
    13141 gattacgtga tgatcgcaat tggcggaact ggtgcagact tctttaaagc acgcggtatc
    13201 aacctagcat atgagctgcg caatttatct gatcagccaa gctttgatga agtccgcaaa
    13261 atcgtaaata tggcaacaac tatgtaccaa aacgaagtat tcgatgaatt atacgtatgt
    13321 tataaccatc atatcaattc gctaactagt cagtttcgtg tagaaaaaat gctgccaatc
    13381 tcagacttag atccagaaga agcaacgact ttcgaacaag aatatatttt tgaaccttca
    13441 aaagaagaaa tcttgtctca gttattgcct cagtatgctg aaagtttgat ctacggtgcg
    13501 atcgttgacg ccaaaacagc agaacatgct gctggtatga cagcaatgaa aacagcaact
    13561 gataatgctg ctaatatcat tgatgagctg acggtttctt ataaccgtgc aagacaaggg
    13621 gctattaccc aagaaattac cgaaattgtt gccggagctt cggcactaga atagtattga
    13681 gattggagga aaaaagatga gttcaggcaa gattgttcaa gtaatcggtc ccgttgtcga
    13741 cgtggaattt tctttagacc aatccttacc agatatcaac aacgcattag ttgtttataa
    13801 aaatgacgaa aataaatcaa aagttgttct tgaagcagcg ttagaattag gggacggagt
    13861 gatccggacc attgcgatgg aatcaacaga tggtttacaa agaggaatgg aagtcattga
    13921 tacaggcaaa gcaatctctg ttcctgtagg taaagaaaca ttaggtcgcg tattcaacgt
    13981 actaggagat acgatcgatt tagaaacacc tttcccagaa gatgcggaaa gaagcgaaat
    14041 tcataaaaaa gcaccagcct ttgacgaatt aagtacaagt acagaaattt tggaaacagg
    14101 gatcaaagtt atcgatttgc ttgccccata tttaaaaggt gggaaagtcg gactattcgg
    14161 tggtgccggt gttggtaaaa ccgtactgat ccaagaactg atccataata tcgcccaaga
    14221 acatggtggt atttctgtat ttaccggtgt aggtgaacgt actcgtgaag gtaatgactt
    14281 gtattatgaa atgaaagatt caggagttat cgaaaaaaca gccatggtgt tcggacaaat
    14341 gaacgaacca ccaggtgcac gtatgcgtgt tgctttgact ggtttgacga ttgcggaata
    14401 tttccgtgat gtagaaggtc aagatgtact gttgtttatc gacaacattt tccgtttcac
    14461 tcaagctgga tctgaagtat cagccttgtt aggacggatg ccttctgcgg ttggttatca
    14521 accaacattg gcaacagaaa tgggtcaatt gcaagaacgt atcacatcta cgaaaaaagg
    14581 ttcgatcaca tcgatccaag caatctatgt gccagccgat gactacactg acccggcgcc
    14641 ggcaacagca ttcgcccatt tggatgcgac aaccaacttg gaacgtaaat taacagaaca
    14701 agggatttat ccagcggtag atccattggc ttcttcttca agtgctttag cacctgaaat
    14761 cgtgggagaa gaacattata aagtagcaac agaagtccaa cacgtattgc aacgatatcg
    14821 tgaattacaa gatatcattg ctatcttagg tatggatgaa ttatccgatc aagaaaaagt
    14881 gttagtttct cgtgcccgca gagtccaatt cttcttgtca caaaacttta acgtggctga
    14941 acaatttact ggtcttccag gttcatatgt ccctgtagaa gaaacagtga aaggattccg
    15001 cgaaatcttg gaaggcaaat acgatgatct tcctgaagaa gctttccgaa gtgttggccg
    15061 aatcgaagac gtcgtagaaa aagcgaaaac actaggctac tagaaagggg tgccccatgg
    15121 atcaatattt aacagtaaat gtggtaactc ctgccggttt ggtctatgat caccatgcag
    15181 ctatcgtcgt tgcacgtacg acagctggtg agcttgggat tttgcctaaa catgcaccaa
    15241 tcatcgttcc attgacgatt gatgaagttc gtgtaaaacg aacagattca gatacccacg
    15301 tcgattggat cgctgttaat ggcggaatca tggaagttcg tgataatgtt gtttcaatcg
    15361 ttgctgatag tgcggaacgt gaacgtgata tcgatgtatc tcgtgcggaa cgtgcaaaac
    15421 aacgcgcaga acgccaaatc gaagaagcaa aagcaaaaca agatacgaac gaattgaaac
    15481 gggcaactgt tgcgttgcac cgagcaatca atcgtatcaa agtatctaag cactcataaa
    15541 atgagatgcg gatgtttatc cgtgtttaag aacagtaaaa agaggttatg acagaagtgg
    15601 tcagcttcaa gacataagaa ggaatttacg aaaattgctt ctcaaatttt tgtgaaatct
    15661 cagcttattt ttcgaagaag ctacttctgg aacaccgtat atcagaggtt gtgaaatcag
    15721 cgttttgtcc tgagtattag agaaacaaat ggaaaatagt tcctcatatt ttgccgtttt
    15781 ttgagctaat accgagggtc agatgattga acaccgttta tttggaataa gggactgcga
    15841 caagactttt gtctcagtcc ctttttttgc atatttttag catttatttc ctaaaaaatg
    15901 taaggggatt cctatatttt taagtatact tgtttttcct tttatacatt ttatggtatt
    15961 atcattttat tagttattat aaaacaatcg ttagaaagga cgtcattatg caagtatacg
    16021 gaattgatgc ggtgcttcga atcgtgagtc atttggcgtt tatctactta gccttctggt
    16081 ctttacgttc gttacgcatt gaaacttttt tcagaccaat gcataatacg caaattagaa
    16141 tggcaatcgt acttttttca atttttttag ggtacactgc aagtaatttt ttcttagaag
    16201 ttatcgcttt atgccgaaat cttttcgtta gcctgtaaat tttaaaaaaa ggtactattt
    16261 taaaagcaag gatgttataa tagaaaagat tttgagtaag tgtatgctca ttggaatttt
    16321 ttggagggaa caacatggaa gagatcatcg taagaggtgg caatcaatta aatggaacag
    16381 taagaattga aggagctaaa aatgctgttt tgcctatttt agctgctagt ttgttagcag
    16441 aagaaggtat tacgacatta gacaacgttc cgattctttc tgatgtcttc actatgaatc
    16501 aagtgattcg tcacttaaac gtagatgtag attttgatga acaaaagaat caagtaacaa
    16561 tcgacgcttc aagacaatta gaaatcgaag cgccttatga gtatgttagt caaatgcgtg
    16621 cttcaatcgt tgtgatgggt ccgctacttg ccagaaatgg acatgcaaaa gtagctatgc
    16681 ctggtggatg tgcgattggt aaacgaccaa tcgatttgca tttaaaagga tttcaagctt
    16741 taggagcaaa aattattcaa aaaaacggct atatcgaagc aattgcggat gaattgatcg
    16801 gtaacacgat ttatttagat ttccctagtg tgggtgctac tcaaaatatc atgatggctg
    16861 ccgtaaaagc aaaaggaaca accatcattg aaaacgtagc tcgcgaacca gaaatcgttg
    16921 atttagcaaa cattttgaat aaaatgggcg ctcaagtcta tggagctggt actgaaacaa
    16981 tgcgtattga aggcgtggat catttacatg cagtcaacca ctcaatcgta caagatcgta
    17041 tcgaagcagg tacctttatg gttgctgctg caatgacaca aggcaatgtg ctgattgcag
    17101 atgctatctc tgaacataac tgcccattga tttctaaatt gatcgaaatg ggtgcagaaa
    17161 tcattgaaga agaaggcgga gtccgtgtca tcggaccaaa acatatctta cctacagatg
    17221 taaaaacgat gcctcatcct ggtttcccta cagatatgca agctcaaatg acagctatcc
    17281 aattagttgc agaaggtaca agtgtggtga ctgaaacagt gtttgaaaat cgtttccaac
    17341 atttagaaga gatgcgccgt atgaacgctc atgtcaaaat cgacggaaat gtagcaatca
    17401 tggatggaaa ccatgaattg caaggtgcag aagtatatgc aacagacttg cgggcagcag
    17461 cagcattagt tttagctggc ttgaaagcaa atggaatcac acgtgtgcgt aacttgaatt
    17521 atctagaccg tggatattat aatttccata tcaaactaca gcaattaggt gcagacgtcg
    17581 aacgtgtaga catggatcaa acttctgctg aaaaaacagc acaaacgatt gcttaaggaa
    17641 gtggctgaat gagtaaccaa cgatatatct tattaacttt gataaagatt ttagtggtga
    17701 tcttattgct tatccttctt tttgtggccg gaacgatgat cggatatggt gtcatcggtg
    17761 gcggcaatcc atttaaagta ttccagccaa gtttgtggat tcatattcga gacttttttc
    17821 attgataaat aagaatcaca gtggttctag tcgatatgaa gtgagccatg aagcagaaca
    17881 tgataaaaga aaacagaagg gaacgttgac gttagtcgcg atcccttttt ttagtaaaag
    17941 aaaagaagga atctaattga tgaagaagat attgatttat ttagttagag gatatcagcg
    18001 ctttatttcg cctgcatttc cacctagctg ccgttattac ccaacctgtt ccaattatat
    18061 gataaaagca attcaaaccc atggtgcgct aaaagggacg gtaatgggga ctgcacggat
    18121 cttacgctgt catccttttg tcaaaggcgg tatcgattat gtaccattga cattccgatt
    18181 aacgcgaaat aagaaagata aaaaagaacc agattataaa aaggttgtga aaaatacggg
    18241 tagcactgag cgataaagaa aaagaggaaa tttattgtca aagtaacaac tccgcaacta
    18301 cttatatggg ggacaaaaaa tgatcgaaga actaaacaag aaagatttac catggtcctt
    18361 gttattagat gcagatcctg ataaagaaaa agttcaagta tatgtttcca gagggagtgg
    18421 ctggatatgg aaagaaaaag aaaaaacaat aggtgtactg atctatgtgg caagggaaaa
    18481 agaatacgaa atcgtcaatg tagcagtaac cccaacgtat caaggaaagg ggattggcgg
    18541 aaaattactt gagacagctt ttcagaagct ttcacagcta gcttcaaacc aaactcggat
    18601 cattatacgt actggcacta ctagtagcgc tgcgttacac ctctatcaaa aaatgggcta
    18661 tgtggagatt ggtcgagtca aagactattt tatccatcat taatctgagc ccatttttga
    18721 aaatggagat caattaaggg atcaagtgat tttagcaatt cacttaccta aaaatagctt
    18781 taactaataa actttcaatt cgagaaaatg ctttcaaatg ctaaatagat acattacaat
    18841 agctttagca aacaaaaagg agaatatttt tatgggaata ttagtttttg attttggtgg
    18901 atcggctgtc aaatatggtt gttgggatgg caaagagatt aaaggtaagg gacaatttgc
    18961 aacgcctgaa tcttgggaag aaatgaaaac gcaattattt caagtttata aaaaaatact
    19021 cgtttctttt gaagtcgaag gaattggaat cagctctcct ggagtagtga ataatgagaa
    19081 acaggtgatt gagggaatca gtgcgattcc ttatattcat ggatttaata ttttccgaga
    19141 tttggaaact ctgtttcaac tcccagtaac gattgaaaat gacgccaact gtgccggaat
    19201 ggctgaattt tatgaaggtg ctgcccgtga ttatcaagat gttgcttttg ttgtggcagg
    19261 aactgggatt ggcggggcgt tgttcacgaa aggacaaata aataaagggg cacatcttta
    19321 cggtggagaa tttgggttga tgttcttaga tggcgataag acattcagta aacttggtac
    19381 agcggtacag atggcttggc gttattgtga acgcaaaggt ctcgataaaa atgcttatac
    19441 cggaaaagat gtctttgaat tagcagaaac tggagatgca atagcaaaag aagaagttga
    19501 gagtttttat acgtatctta caaagggatt attcagtatt caattttcgt ttgatccaga
    19561 agtgatcgta ttaggcggcg gggtttctgc aaaagaaggg ctcattgatg agatcaaaag
    19621 ccgaatgaag aagctgacag aacaattcga tctgcatgat ttcgaaccgc agattttatt
    19681 atgtgagtac agaaacgatg ctaatcttgt aggtgcagca gcaaactatc tcgctgtacg
    19741 tcaaggagag aattaagagc tttagagaag gtgcagcttg cataaaagaa gagaatgaaa
    19801 tgaataggag atttttacct aaggaatgtt ttatttgctg cagtatgata aaatatctga
    19861 gaggtgaaaa tgatgtcaaa aaaaaataaa gatattgaag tgcgtataga agaagtgaaa
    19921 aaagatatca aaggtaagaa ttatgaagtc actcagttat ttatagggaa aaaaatgatt
    19981 ggtgagatct tagcctatgg gccaaaagaa tatgaaattt tctttggtga agaagatttt
    20041 ggaaaagaaa aaagtcttga aaatgcgata gaaacagtga ttcgacactg gaatctgcac
    20101 gaataaaaaa atatcaaaaa aactttcaat aaagggttgc aaaaatctgg aaaattaggt
    20161 attataacta agtcaggttg atacaaacct ttcggaggag tagcgaagtg gctaaacgcg
    20221 acggactgta aatccgttcc ttagggttca gtggttcgaa tccactctcc tccatttcat
    20281 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
    20341 cagctacccc agta
//
LOCUS       DASHRQ010000051        19593 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.054, whole genome shotgun sequence.
ACCESSION   DASHRQ010000051 DASHRQ010000000
VERSION     DASHRQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.054"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            133..3660
                     /locus_tag="U2M88_001978"
     CDS             133..3660
                     /locus_tag="U2M88_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEM8374341.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="U2M88_001979"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="U2M88_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEM8374342.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="U2M88_001980"
     CDS             7448..7789
                     /locus_tag="U2M88_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8374343.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="U2M88_001981"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="U2M88_001981"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEM8374344.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="U2M88_001982"
     CDS             9146..11566
                     /locus_tag="U2M88_001982"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEM8374345.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="U2M88_001983"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="U2M88_001983"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEM8374346.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="U2M88_001984"
     CDS             13000..13737
                     /locus_tag="U2M88_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEM8374347.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="U2M88_001985"
     CDS             13751..14566
                     /locus_tag="U2M88_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEM8374348.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="U2M88_001986"
     CDS             14579..15226
                     /locus_tag="U2M88_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEM8374349.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="U2M88_001987"
     CDS             15242..15898
                     /locus_tag="U2M88_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEM8374350.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="U2M88_001988"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="U2M88_001988"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEM8374351.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="U2M88_001989"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="U2M88_001989"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEM8374352.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="U2M88_001990"
     CDS             18240..18758
                     /locus_tag="U2M88_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEM8374353.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVIQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="U2M88_001991"
     CDS             18865..19365
                     /locus_tag="U2M88_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEM8374354.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6579 a         3363 c         4205 g         5446 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtga ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc ttt
//
LOCUS       DASHRQ010000052        19534 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.055, whole genome shotgun sequence.
ACCESSION   DASHRQ010000052 DASHRQ010000000
VERSION     DASHRQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.055"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..209)
                     /locus_tag="U2M88_001992"
     CDS             complement(<1..209)
                     /locus_tag="U2M88_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEM8374355.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSD"
     gene            complement(308..622)
                     /locus_tag="U2M88_001993"
     CDS             complement(308..622)
                     /locus_tag="U2M88_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374356.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            complement(690..1184)
                     /locus_tag="U2M88_001994"
     CDS             complement(690..1184)
                     /locus_tag="U2M88_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374357.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            complement(1177..1491)
                     /locus_tag="U2M88_001995"
     CDS             complement(1177..1491)
                     /locus_tag="U2M88_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374358.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            complement(1707..3098)
                     /locus_tag="U2M88_001996"
     CDS             complement(1707..3098)
                     /locus_tag="U2M88_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEM8374359.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            complement(3083..3694)
                     /gene="mobC"
                     /locus_tag="U2M88_001997"
     CDS             complement(3083..3694)
                     /gene="mobC"
                     /locus_tag="U2M88_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEM8374360.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            complement(3698..3895)
                     /locus_tag="U2M88_001998"
     CDS             complement(3698..3895)
                     /locus_tag="U2M88_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374361.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            complement(3886..6300)
                     /locus_tag="U2M88_001999"
     CDS             complement(3886..6300)
                     /locus_tag="U2M88_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374362.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENVQNSTSSQGFKTVDVRENIQKDSGEMKEKTQKINIVEDRK
                     SAKKFDSNHETVIIDSEIRENDKGSKSRRRFTYEDNELFRDTLNDPNPLFDNLLRNKR
                     GGLSCN"
     gene            complement(6287..6505)
                     /locus_tag="U2M88_002000"
     CDS             complement(6287..6505)
                     /locus_tag="U2M88_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374363.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            complement(6581..7633)
                     /locus_tag="U2M88_002001"
     CDS             complement(6581..7633)
                     /locus_tag="U2M88_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEM8374364.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            complement(7786..8112)
                     /locus_tag="U2M88_002002"
     CDS             complement(7786..8112)
                     /locus_tag="U2M88_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374365.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            8137..9303
                     /locus_tag="U2M88_002003"
     CDS             8137..9303
                     /locus_tag="U2M88_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM8374366.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            9717..12944
                     /gene="fss3"
                     /locus_tag="U2M88_002004"
     CDS             9717..12944
                     /gene="fss3"
                     /locus_tag="U2M88_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEM8374367.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            13057..13371
                     /locus_tag="U2M88_002005"
     CDS             13057..13371
                     /locus_tag="U2M88_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEM8374368.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            13384..13758
                     /locus_tag="U2M88_002006"
     CDS             13384..13758
                     /locus_tag="U2M88_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEM8374369.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            13768..14103
                     /locus_tag="U2M88_002007"
     CDS             13768..14103
                     /locus_tag="U2M88_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEM8374370.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            14186..15526
                     /locus_tag="U2M88_002008"
     CDS             14186..15526
                     /locus_tag="U2M88_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEM8374371.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            15604..16278
                     /locus_tag="U2M88_002009"
     CDS             15604..16278
                     /locus_tag="U2M88_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEM8374372.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            16271..16435
                     /locus_tag="U2M88_002010"
     CDS             16271..16435
                     /locus_tag="U2M88_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374373.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            16511..17653
                     /gene="dcm"
                     /locus_tag="U2M88_002011"
                     /pseudo
     CDS             16511..17653
                     /gene="dcm"
                     /locus_tag="U2M88_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            17643..17933
                     /locus_tag="U2M88_002012"
     CDS             17643..17933
                     /locus_tag="U2M88_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374374.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            18192..19376
                     /locus_tag="U2M88_002013"
     CDS             18192..19376
                     /locus_tag="U2M88_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEM8374375.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            19373..19510
                     /locus_tag="U2M88_002014"
     CDS             19373..19510
                     /locus_tag="U2M88_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEM8374376.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         6208 a         3440 c         3559 g         6327 t
ORIGIN      
        1 aaatcagata cataagccca tgtacccgtt cgttgtaaat aaagttcttc ttgtaacaac
       61 tcccattttc tcttagaaat cataatgacg ccatcgttca atccattttt agaatcccta
      121 gtaatttcta tttcagaatt tgaatgaact acttcttgca acactttacc tatattttct
      181 ttgaagtttg ttggagtaat agtatccaaa atgaatacct ctcttctcta tacctctatt
      241 atgaagtgcc cataaaataa agcaagcgtt gaggaataat tctatcaaca cttgctttat
      301 taagctttca ttttgtagtt tgactaaaac caataatctt atttctaaac tctcgaaaaa
      361 cttcttgtgg ttgataatag tctggatcta tttcaaatgt ttgtgagaat aattcattga
      421 atttttctac tgaaataatg attttaccat tcttctctcg actgttttct attatttctg
      481 cttctaattc aaataaatct agtgacgaac taaaatcata aatcatgtca tattgaataa
      541 aaaaatcaag aaaaagttgc tcaaactctt ccttatttat cactaatgtt tcaaacggaa
      601 caactttatt ttcctcagcc attctcccag ttccattttc aaaaatcaca aaactgatat
      661 taccttaaaa actactaaca aaataaaact tataaagtaa atggcaaccc aactatgtaa
      721 ccattctctc tacgtactac gatttggtag tcctgccttt caatcggtaa ttcataatag
      781 taatttactg tttcttcttt gccactttca atatactctg gattatccaa tatcatatca
      841 tactcataaa gttcttcacc atccttattt tcaatcgacc attgttgtgg acgtatcgga
      901 ttttctgtat catttataag tgtataggtc acttgtaata cacgatcatg ttgtgcaaat
      961 tcatttcttt tttcggtcac ttcaatattt ttaaatgtta gttcaacccc atctgcctga
     1021 taagtttcac ctatttttaa atcttttgtt acaattttta ttggcttttc ttctggaaaa
     1081 tgtctggctt taattgttag ataacttcca aaaagaatca atcctagtag cactaaaaga
     1141 attcctttaa cgagccactg aaacgggaag taaactttaa acatcttttt tctcctccgt
     1201 tttgtatttg ttttttagag aatacgataa cttttgatca atgtcatcca gcttttcaat
     1261 tacttcatta cttatcattg attctagttc tgaaattttt tgttcaaatt taatgctttc
     1321 aggatcatta aatttattat ttgatggact agctaacact ggtaaatttg aaaccataac
     1381 acgaaacata tttgcctttg aaccaaaaat tcctttgttt tggttgtaaa aattgttgaa
     1441 tatttcttta gtttcttctg aagccgagac tttaaaatat ggatagttca tttttttctt
     1501 ccacctttct ttttttggga gggagtctgt agggagcttg ctccctcaga gatttacccg
     1561 ccatctgact tgccagaaag tagcctagcg tgagcggggc ggattgactg gcaagtagat
     1621 gggtagctgg cgtatgtcct aaaaactgct tctttccaag caactcttca taggcaaaaa
     1681 actggaaagt ccactaacaa gcccattcag aattccattt caaaattttg ttgccttttt
     1741 tgctgatttt caatcatttg ttgtgtgaat tcataatcct ttttagcacg atacattttt
     1801 acatcgtcat tcattatgcg actgatttga tagtgaatct gtttagcaga aggtgtatga
     1861 gaacaatttt caaaaccctt tgccggtaga aataactctt tctgatcagt ctttttaata
     1921 gaaattgttt tacttttcgc actacgtaaa gtttgaggat tcacatccgt atgaagttta
     1981 atcgtgtatt cttgatcatc ttctaaagac attgtattta caaaatgctt gtctacctct
     2041 cgcaagaagt catcatattt aatgaatttt tcatcgcctt cacggtcgct aaattgcttt
     2101 tgtgcgatgg aatctaattc cacaaacggt acatttaact caccagtttt gtaatttact
     2161 ccactaatta gcgacataac ttgctctctt tccatattaa aaaaattttt tatatgtgat
     2221 tcttctaaat tcttttcaag ttgttcagaa acagattcaa atgaatatct tttccctaac
     2281 tttcttgcac taaaagtacg ttcgacatac tgactttttt cttttctaga ttgtttatct
     2341 cttcctaaag taattaactc acctctacgc tcatagaaat ttacaccttg tttgtaaaga
     2401 acatagccaa gtttgttatt tgtttttgaa aattgataaa cttcaatgtt atattctttt
     2461 gccaaatgat ccgaaaaatc ttcaaaagaa ttcacttcgc atttttccaa cgttgcttcg
     2521 atcgcatttt gcacaaactc ttgataatta atttttccat atttcatctt agaaggatcg
     2581 tttgaataaa gattggttct cttttctaag ttttcatagt actcattatt cactgattca
     2641 agaccatgtt cattgaggac ttgatcgtta atttttcgca ttagttctaa atctttaggg
     2701 tcaatacgca accttttaca tgtttccatg ttaatattat taatgacaag atgactatga
     2761 aaatgatctt tatcattgtg acttgcaaca atgatttgcg aatttggaaa agctttctct
     2821 gcaaattcga cagctatttc gtgcatttta tcaatactta actctttaga agtactctta
     2881 tcaaacgaat gaataacatg aaacgcttgt gctatatctt tagatttatt catcttggta
     2941 cgtactaaat tcatttcttg cagtgcttcc tcactatcat cactacaatt tattcctgag
     3001 actgcaacac atttttcttt ttttgaagga ttagctgcat attgtaatag cgaggataaa
     3061 tttttcttgc gtcctttcga tgttatagta gctcccaaag ttgtttcacc tcatctttta
     3121 aacttgcaat aatctgaatt gagtgaatta aattccgatc aactttttgg gaatactctt
     3181 ctttcgtacc cggtctagtt tgcttttcta tccaatattc gaaatattct ttttctactt
     3241 ctgataattt aaaaaaagag ggggcactcc gattggactc caaaaaaatg tcatctggat
     3301 aactaatcat caactttttt gctatgctat tcgcaagttc atctttaaaa atgttgtaac
     3361 ggctagcatc aaaactaaaa tgtaggctct tttctttttt atcttcagat tcttctctaa
     3421 aatatttagc aatgatattt aatgccttta ccgcttgatt caaattgtta ccaatttgat
     3481 ttaactgttt ggttaaaact accctatcat cttcagctaa caacggcggt tcatatttga
     3541 gtccctttaa ccgctcacgg caaacgtctg ctctagaaat tcccctttgt tttgcaatca
     3601 atgtcagttc aagataagtc tttttatcga cacgaaaatt taaggcctca ctatgtttgc
     3661 gatttttttc gtacattttt tgtttatcat ccattgatta tcccctcaca tttttttcta
     3721 gagagcaaca tttgaaattg ctctctattt catttttcat ctttaattct tcttgaatca
     3781 tcagttcaat ataattagtg accgtgattt tttcatttgt tgcgttcttt actgattcac
     3841 ataattcgtc aagttctaag aaatcatgcg tactaatctt tagaattaat tgcatgacaa
     3901 tccccccctt ttatttctta ataaattatc gaaaagtgga ttaggatcat taagtgtatc
     3961 tctgaataac tcattgtctt cataagtgaa tcttcgtctt gattttgatc ccttatcatt
     4021 ttcacggatt tcactatcaa taatcactgt ttcatgattt gaatcaaatt ttttcgctga
     4081 ctttctgtct tcaacgatat tgattttttg tgttttttct ttcatttctc cactatcttt
     4141 ttgaatgttc tcccgaacat ctactgtttt gaatccttga ctacttgtag aattttgtac
     4201 attttctcgg acatctactg atcggccacc atgactactt gtagaatttt gtggattttc
     4261 tttgactaat ttttctaatc ctgtatttga atttttagct tcttgaattc tttctttggt
     4321 caacgcttct tttccgtcaa tattagcgcc tgtccgccct tctacacgtt tccttaacat
     4381 tatttcctcg cgattcattg cttcaggcgt gtatttcgct tcatgacgct tctgattaat
     4441 taattcttca gcagacttag cttgaccgtg catagcattt ttacgttctt caatatcttg
     4501 ttggactttt tgctgaattg cgcttgcagg attattattc gattcagcat tatttttcat
     4561 tgcttcacgc atacgttcag aactagacat aattctatcg gcattttcaa ctttagcacc
     4621 atcttttcta tctgaaagac gtgttggcac ggaatcagca tattttccag tttcatcttc
     4681 tttattttta gctaacatag gtttgctatt tttattaata gcggacactg taccatcatc
     4741 taaaccttct ttaaacttat tcttggtatc attagctata tcacgcatac tcttaaatgg
     4801 ttttgttgct ctttcactag ccattgtagc tccatctgaa gctaaaccgg ataatccacg
     4861 ttcatgggca tatcccaaag ctcttccagt tttgccagct ttttttgata gagtttcgcc
     4921 gaaattcttt tctggttttc gatcttctcc ttctccgctt cctttagcac gattaaatcc
     4981 atttgatcct tttctaaaca gcattgcgcc cattccaaaa gcagaagcaa gttgcccata
     5041 gccttctttg agtccaatat caacaccaaa gtatctcatg actgttccac tgcctttgat
     5101 taaaacaaaa acggcacaga tataagcaat tgtttttata aaaatatttg ttgagatacc
     5161 ttgtcctgag ttcagaaaat tgataaacat agcaaacatt gataacccaa atccttgaaa
     5221 cccaacagtt aagtaacatt gtagaatatc tgatagaaca acttttgaac gctgtcctgt
     5281 ctcaatatct gttgcaaaaa caagaacacc taacacacgt ttaaatgcaa gttctagtat
     5341 tgctgtaatg atcacaaacg cgctgaacac ataggcgatt gctaacgctg tcaacccaat
     5401 tgaaatcccc catagatttg cttggtatcg gtaatagcct gatttcaagc tatcagaaaa
     5461 tgttgataag aagttgtcag aaaactttgt agcaacaaat tcattattag aattttcaac
     5521 taattcataa cgaagattgt ccgcaattgg attttctttt tccaatttat caatcacttt
     5581 atccgttaac acttgtgtga gatccgtctg ttgaaaattt tttcgtgtta aattatttcg
     5641 atcctccgtt ttatcaatgc ttgagtactg atctgtttta ttaatatatg ctaaatctgt
     5701 tactccttgt tttatcaatg accaagcgac tccgtcatct gaatttgtaa tcgtagtaga
     5761 gtccttataa aatgttttag caaattgaat accactagaa atcatggttg gcattaaaag
     5821 tattaatgct atagacatca ctacgttcat tccaaccgat tttaaatcca ctgttccttt
     5881 tccagtaatc attttgtaac caacaaacat caatgaaagg atcattaatc caatgacaat
     5941 tgtatttacg gctgattgat aaacattatt tagccctgta ctttcaatga aattgaacaa
     6001 atccagcaca tctggaatca tattttgaat cgaatctgtc acagtaaaca tgccttttac
     6061 cacagcccat ccgagccaaa tcccgaactg actaaggaaa tctccattgt acttttttag
     6121 gtaaggtcta taaagagaat tattcaaaaa ctctgtttga ctttttgtgt catcaaacca
     6181 attgaaaata ctcgcatgag caatattact tgtaaaaata atcatagcta tcagaacaag
     6241 aaaattaaca accaaccaat ttattttttt attggcaatc tttttttcaa ttattttcat
     6301 cgttacctcc cttagcccat tttctgaggc tctcagagag cttctgagcc attttttgat
     6361 taattatgac atgagttaca caattatcat tttcagctct taaatcgaga actatagtgt
     6421 tgaagcaaat agtctcgatt aattcgagac tatctgtatt ttgatctaga caccaaactt
     6481 gatctattaa acgattttta atcaaaataa cccctcctat attcaaagtt ctcaaatgaa
     6541 gtggagatac ccctccattt catttgactc aattctattc ttattgctta tctattcctt
     6601 cgctgatttg caattcaatt agttctaacg ctttattttt tagagaagca aagatataat
     6661 cgtctttttg tttgatacga tgatctgttt ttgctcttcg tatcaagtca cgtaaagtca
     6721 ttgatagctg ttctctaaca tgatctacaa aatacttgtt attacaaatt tcatagataa
     6781 ttttcagttg aattttttgt tctttttcag cagatctaat cgcatatttg aattttttgt
     6841 accagttgtc gaattgacga taatcactat tgctagccag ttttaagcaa ttcacaatac
     6901 tttctcccca tatttgttta agaagatttt cttcaataaa tgtttcgatc aattcttcat
     6961 ctaatttatt atttttagtt gaaagtgatt caagaagatc atcattatgt gattctttaa
     7021 tatctttact atctttataa gacttatata attctagttc attttttgaa ctatcagcaa
     7081 aattgttttt gtctcttgtt ttcattgatg tgtcgcatag ttcaaatttt gaactatcaa
     7141 cagaatattg atttgttcca tagttcaaat tttgaactat caagtttttt tgtctatttt
     7201 cttgatgggt tatcttatta attttataaa catcatcttt gcacagaaca ggctttaaca
     7261 aataataaaa atttgggaac atttgcattt tacttttatt gaagccattt tttcttttca
     7321 caagaagttt aactttttga agttcatcta gtgctgataa gattgtattt tttgacattc
     7381 ccgaaatttc tacgagattt ttgatttcaa atatcaaaaa aattttttga ttctcatcat
     7441 acaagttatt tttcaatgaa agtctatttc tatcttttag taacatataa actaacttag
     7501 aacttagttt cattttctta tatttttcat ttacgtttaa aaaaatagga tattgatagt
     7561 ataattctgc atatatatca gaattagaat agccttgctt ttcaactaag ctttcaactt
     7621 ctttttcatt cattgtcaca cctgcttctg attttatttg ctataataga ttcaaataaa
     7681 gttttctcag aactttatta ttgagtgttc ccccgatctg caaactgacg ggaacactct
     7741 ttttaatttt ttctacttca tcaaatttaa aaaacgaggt gttttttaaa tttgatgact
     7801 caatatccat tttttgcatt cttcttttga aaacaactcc attttttcta ataaaataac
     7861 tggcaatccc attcttttaa atttcaaaaa agttgtatat gatacattca aatatttagc
     7921 catctctttt ccttttagaa atggtctttc taatccggta tcaattcttg cttgatcaat
     7981 agcctctttt gtcatttcaa aaacataatt tttcaattga ttcgtttgtt gtttatctaa
     8041 cattactgta attcctgcat ccattatttt tctcctctca ttttctattt cttcaatcag
     8101 ccaactcgcc aagttatcag attggagaga ttagttatgt caatacaaaa gtacacaaca
     8161 aaaagcggtg cacgatatag tgtccgcttg tatgttcgta aagatgaaaa tggaaagcaa
     8221 gaatactatg taaaaaaagg gtttaaaact gaaaaagaag ccaagttaca cgaagcaaga
     8281 aaaaaggtcg agattgaaga taccggctat cataagcctt ctaatgactt gtttataaca
     8341 gtgtatgaag aatggttgcc ttcttatagg aatcgtgtac aagaaacgac atatcaacgt
     8401 acaaaagacc ttttccgctt gcatatacta ccaaaattcg ggaataagtc catttctaag
     8461 attactcctg ttcactgtca gaatgcagtc aatgaatggg ctttaaaatt caagaatatc
     8521 aaacagctta agtcttacag ctctcaaata tttgagtatg ccatttttgc agagctatta
     8581 caccgaaatc ctatggcaaa tacaaatcta ccaaagcggg aaaagaaaga aaatgaaaat
     8641 tacttttcat tagaagaact aaaagctttt tgggatattc tcaataaaga agagccttta
     8701 aaacatattt taatcttcca gcttttgatt actactggta tacgtaaagg agagctagct
     8761 gctcttcatt ggtctgacat ttattttgaa gaacagctaa tgtatgttag aaagagctat
     8821 gcaactattc gaaatgaaga gaagaatgca agaagaaaaa ctgtcaggat tcaaaaaaat
     8881 acaaaaaata tatcttccga acgaatatta ccaattgatg cacaaactat tgaaatgtta
     8941 aaaatctgga aaaaagaaca agctcgagag cttttgcagt ttggcataaa tacaaataca
     9001 aaaaatcaac ttatctttac ctatgtaaat gcagacggtg acattaatca acctttgcat
     9061 gcggactatt caaataatat attgaaacgt ttagagaaga aatataaatt taagcacgtt
     9121 actattcatg gattccggca cactcacgct actcttctac tagaaggtgg agcaagtatc
     9181 aaagaaacac aagatcgatt gggtcataaa aatgctgaaa ctacccttaa cacttattct
     9241 catgttactg aaaaagctca acgaaatgcg ttagaaaaat tcattgctta cactggttta
     9301 taatattgtc attacctttc tcattaccag aacgaaaaaa agacaccttc tcgcaatgag
     9361 aaagtgtctg aaatcgttgg tataacaacg attaacgttt tgaaaactgt gaagctttac
     9421 gtgctttctt aagacctggt ttcttcgtat cattgaataa gtttgaaaaa ccttgattta
     9481 taaaggtttt cccgtaaata tattttatta tatttgatta aatttgaaat gtttgtgttc
     9541 taaaatgtgt tctaatttaa aggcagtata tttcgtattt tagattaaat ttttaggctc
     9601 attcattctc aaatccattc tattcagtca ataaaaattt taaaaagact ttataaacaa
     9661 agatgttcaa gcgagcatct tatttttttg tgcttaattt gaaaggagaa catttcatgt
     9721 ataaaaaaca aaaaaagaaa ttatgtgtat taactatttt gagtttggct gttcaagcaa
     9781 ttgttggagc aattgctcct acgattactt ttgctgatga aattacccat ccacaaacgg
     9841 taaccgtaga actagattta gcacatcaat atgctgttga aggaactttt agtgatggtc
     9901 gtccaatgtc agaagtcact gttccgcatt atgcagtgta caacggcttt aaacaagata
     9961 ttttctgtat tgaaccagga gtcccgattt acaatgagtt cactcctggc tatgagaaaa
    10021 acccgttacc agatatgtcc gataaagcca aattagtttc cgttttatgg aaaaaagtag
    10081 gtacagatgc agatactcat attgtggcac agaagatgat ctggcaagaa gttaatgggt
    10141 atacccttca ctcaataaaa cgatcagatg gtagtgccgt aaatatcgca gccattgagg
    10201 caaaaataaa tcaagccatt gacgattatc agaagaaacc aaccttccat aatagtacgg
    10261 ctaaaacagt attagggcaa tcgactactg tgacggatac gaataatctg aatttgtcag
    10321 agttcgatga ggttgtggaa aatgccgcaa acattgatta tcgtgtgaat ggtaatcagt
    10381 tgataatcac cccaaatgcc aattcaaaag aaagtggtgt gctgacgctt aagaaatctg
    10441 ctggtaccgg aacacctgtc gcctataaaa aggctggcca acagacttta atggccgggg
    10501 cgattgataa acctaatacc tacacaatca aaattgatgt ggaaacggag ggttctttaa
    10561 agatcaaaaa agtcgataaa gaatcgggtg atgttgtacc aggaacggtt ttccatttag
    10621 actttggaaa aactttacct gcaaaagacg tgactactga caaagaaggc attgctacat
    10681 tggatgagat tcctcatggt acaaaagtaa ccattaccga aaaatcagta ccggcgcctt
    10741 atacgattga taccaccccc atgactacca ccattaaagc aggcgaaacc atttacgtga
    10801 cttcaaaaaa cgcacgggaa aaagggcaaa tcattctgga taaaagcgga gttgaaacag
    10861 ggagtgatct ttggaatgac aattattcct tagctggtaa tacgtttgcc attcgcaaag
    10921 acagtccaac cggtgaaatc gttcaagaaa tgaccacgga tgaaaacggt catgcggaaa
    10981 caccaaaaga gattgctaat gcgttggaat tgggaaccta ttatgtgacc gaaactaaag
    11041 ctagtcatgg gttcgtgaat acgttcaaac ctgtaaaagt cgaattgaag tatgccaatc
    11101 aaaccgtagc ccttgttacc agtaacgtaa aagggcaaaa tcaagaagtc actggcgaaa
    11161 ccactttgac gaaagaagac aaagatactg gggataaagc acaaggtaag gcagtctttg
    11221 agggtgctga atacaccttg tttactgcta aggatggaaa agctgttaaa tggagtgagg
    11281 cttttaaacc agagttagcg aagggaacga aaggttctga tgaaacggtc actttgactt
    11341 tagacgaaaa gaaccaagtt gccgttaaac atttagctat caacgagtat tattggcaag
    11401 aaacaaaagc gcccgaagga tatagccttg atgaaacaaa gtatcctgta tccatcaaaa
    11461 aggtggatga tagcgaaaaa aatgccgtga ttacccgaaa tgttacggca aaagaacaag
    11521 tgattcgctt tggctttgat ttctttaaat ttgctggatc agctgcgggc actaccgaaa
    11581 ctggcttcaa tgatctgacc tttaaagtgt cgccattgga agggaccaat gaaatcacag
    11641 gtgccgaaga tgaagcgacc acagcttata acgagcaatt agggtttgat ggttatggca
    11701 agtttgaaaa tcttccttat ggggattatt tacttgaaga agtagaagcc ccagaaggtt
    11761 ttcaaaagat tacgccatta gaaatccgtt ctacttttaa ggaaaacaaa gaagactttg
    11821 tgaagagtga gtatgtcttt accattaccg aacaagacca aaaacaacca attaaaacgg
    11881 tgaccgttcc ttacgagaag ctgacgaaca aagcattttc tgttagtttg aaccgtttaa
    11941 tgctctatga tttgcctgag gaagaagata gtttaacttc tcttgcgact tggaaagacg
    12001 gaaacaaaga attaacttct ctggattcta ccgagctagt tgataaattg agttacaatc
    12061 tacatgaaat caaagaagac tggtatgtgg tagcgcaagc cattgatgtg gatgcgacaa
    12121 aagctgccca agaaaaagat gaaaaagcca aaccagtggt gattgcagaa acaagcgcaa
    12181 cgttggcgaa caaagagaaa acaggaactt ggaaaattca gcataaatta accgccgaac
    12241 aagttttaaa taaaactatt gtcttattca actatgtgta tgaaaataag gaagcttttg
    12301 aagcaggcga taagccagta gcgaaggatg ttagcttgaa taaccaagcc caaactgtca
    12361 gttgtacagt ggaacaccat gtttctatcc aaacaaaggc ccacctagaa aacggttctc
    12421 agacctttac tcatggtgat gtggtggata tgtttgatga tgtgtcgatt actcatgatg
    12481 tactggatgg ctcaaaagaa gctttcgaaa caattctgta tgctttatta ccggatggta
    12541 caaacaaaga aatttggaaa tctggcaaga ttgattatga agtaaatgat aaagaattca
    12601 ccaaaactgt gcttgcgaaa aaagtagata ccggaaaata tccagaagga accaagttta
    12661 cttttgcgga aatcaattac gataaagatg ggaatatcaa tggaaaacac aatgaagatt
    12721 tgaaagaaaa atctcaaacc ttaacaccaa aagaagttcc aaccatacta agtacgccga
    12781 aacaaccgga aacaccaact gttccaagtg gctctcaaga atctagtccc acagtgaaga
    12841 ccttccctca aaccggggag aaaaattcca atgttttact gttcattggt tttactttga
    12901 tttttgcgac ggcgggctat tatttctgga atcgccggaa ctaaggtgat gagatgatac
    12961 aagtaaatcc taagaaccaa cgtgcgcgac cgcctcctaa aaaagtagtc gcgctatttt
    13021 tgtatccaaa actaacaatg aaaagaggaa aagaagatgg aattgaaatt tgtcgtgcca
    13081 gatatggctg aaacctttgg aaagttaagt tatgccggtg aaggagaaat tataacagaa
    13141 ggatatggac ggaatactac ggtgattggt cgcagttatc atttgtattc cagtaagcaa
    13201 cgggccgatg atattgaggt ggtagtagct gcggaagccg gtgaaaagga ttttgatcaa
    13261 gaccaaccgc taaaagccgt gaatccccat ttggttgcca aaggctatga gattgagaat
    13321 cgtgggttta ctgattacgt gttgtatgtt gatgatttag tgaaagcata ggaggaaaag
    13381 aagatgagat taacagaagg tattgtcgta gattcaggtt taacatttgg gaagttgcgt
    13441 ttttccgcat tacgccgaga agtacggaag caaaatgagg atggaacaat tggtaatgag
    13501 gtaaaagaac gaacctataa tttaaaatcc tctgcacaag ggcgaatgat tcaagttagt
    13561 atcccagcta atgtaccctt acgcgagttt gcttatgatg cagaggtaga gttggtcaat
    13621 ccaatcgtgg ataccgttgc caattatgtt tttcgagaag gaactaccgt taattggttc
    13681 attaaggcgg atgatttagt cttgaagcgg cagccgaatc aaggaaatcc tacgaaccaa
    13741 aatgaaagaa agaaataggt gaagattatg gaaattaatg ttgaacatat tttagattgt
    13801 ctcgatcaat atggaaaagg cgagctgact gaggaacaac tcgttaaagc attaacctct
    13861 gatgaaaaaa tgtttctgat tatacatcaa ggtttacttg gagaaaatag cgacagtgaa
    13921 gaagactctg atgtactaga ctggttagga gaagaagagt ctttctttat agttgtcgaa
    13981 gtaaatgaaa acttatgtcg gcaggcagaa tccattttgg aagaaattgg tgtggagatg
    14041 ccggatgcta ttgaaggctt tttaaatcaa ttggttgaaa cgaaacaact cccctcctcg
    14101 tagcagtaaa tgactagcat aactttttga aattgcctaa tgaaatccta ttgggctttt
    14161 tcgattggag gtggaaacca tcaaaatgta caaaggacat cgcatacgcg caggagatca
    14221 gcacttggtt tatcactttg ttcttggctg gctactcgct ctgttcatcg gttggatgag
    14281 cgtcttttat tttcaagagt ttagacaatt tgatatttct aagctatcac tttctactat
    14341 tgaaatcgtt tggtctataa aagatttagt atgtctgctt ggaagtctcg ctttttcagg
    14401 agctatgata ttgctttata ttcacttttt tcttgatcat tggcgaagct tgtggcatcg
    14461 acaaaagcta gctcggatga ttctagagaa ccattggtat gaagtgaaac aaactcaaag
    14521 tgaaggcttt ttcaaagatt tgaatagtag ccgaaccaga gagactatca gttacttccc
    14581 caaaatctat tatcgaatga aagatggatt actgtctatt cgcgttcaaa tttcacttgg
    14641 gaaataccaa gatcagcttt taaaattgga aaagaagtta gaaagcggac tgtattgtga
    14701 gttagtagaa aaggaactca aagactccta tgtggagtat actttactgt atgatatgat
    14761 cgccaatcga attgggatag acgaagtagt ggcagaaaat gggactctgc gattgatgaa
    14821 aaatcaagta tgggcttatg attctttacc ccatatgtta atcgctggtg gcacgggtgg
    14881 agggaagacc tatttcctcc tcaccattat cgaagcacta ttgaagtcag atgcagaatt
    14941 gtttatcctc gatcccaaaa atgcagattt agctgattta ggtacggtga tgcctcatgt
    15001 ttattcacaa aaggaagaaa tttctgcttg tgtggaagat ttctatgaac gtatgatggc
    15061 tcgtagcaag gcaatgaaag aaatgtctaa ctacaaaaca ggagagaatt atgcctatct
    15121 tggacttcca cccaactttt taatctttga tgaatacgtg gcttatatgg agatgttaac
    15181 gacgaaagaa agtgcggtga ttttgaataa gctcaaacaa atcgtgatgt tgggtcgtca
    15241 atctggtttc tttctgattt tggcttgcca aaggccggat gcgaaatatt taggagacgg
    15301 aatccgcgat caattcaact ttcgcgtggc tttgggtcgt atgagtgaac tcggatattc
    15361 catgatgttt ggggaggtcg aaaaaaactt ctttatgaag cacatcaaag gtcgaggtta
    15421 tgtggataca ggtggcagtg tgattagtga gttttatacg ccacttgtac caaaagggta
    15481 tgatttctta gattcaatta gtaaattaga aaagatgaat gattgagtga tcttcaatac
    15541 tgactttata cttcaagtaa cgtataatac ttttatatag taggaagaaa ggggataatt
    15601 aatatggaaa gtaatgattc ttatttagag tatgcaaaag caaataagga taagtttatt
    15661 tcttatatta tcaaagggaa atcccccgaa gttggacgag acgcagtttt tatggcaggg
    15721 agtcctgggg ctggaaaatc agaggttgcc attggattag ctgagaatta tgataaccat
    15781 gtgattattg atgcggatta ctttcgcacc cagtttcctg attataatgg aaaaaactct
    15841 agtctctttc aaaaagcatc ttcttggttg gtagagcagg catttagata tgtcttagat
    15901 catggctatt cttttattct ggatgcaact tttgcaatat taagtgctga aaaaaatatc
    15961 tcgcgggcgt tgaaaaaaca ttttcgagtg acgattttct atgtttatca agaccctaaa
    16021 atcgcttgga aatttacaca agaacgtgaa attgcagagg gacgtcatgt gccgaaaaag
    16081 gcatttatta acgctttttt caaatcaaga gagaacattg caaaagtaaa gagtaaacat
    16141 ccagatgtat tgcttcatat cataataaaa gattatacga ataatatttc cgaagttcat
    16201 tatgctacgg ataatctaga attattttta ccactagggt atagtaggaa agatttggag
    16261 gttcaattac atgactgaga aagaacaagt aacgaaaatt gtaaagaaat acaataagag
    16321 cattgcggat ttatcggaaa atgctactgc aaaagagttc aaaacggtga ttaaatatgt
    16381 agccgatcag gccaatgaaa aacaacggaa attagttgga ttaaataaaa aatagtagga
    16441 caaattaata atgtatgagc caagagcaaa atgttcttgg cttttttctt tgcaatgaat
    16501 gaggtaatgt atgagatttt tagatttatt cgccggtatt ggtggctttc gtttaggtat
    16561 ggaacaggcg agccatcatt gtatcggttt ttgtgaaatt gataaatttg ctagacgtag
    16621 ttataaagca attcatgata caagtaagga ggtggaaatg catgacatca caagtgtatc
    16681 agatgagttt attcaatccc tcggaccagt ggacatcctt tgcggcggat ttccgtgcca
    16741 agctttttca attgcgggaa agcggcaaag attttccgat actcgaggta ctttattctt
    16801 tgaaatcgct cggttcacca ctcttctccg acctaagctt ttattccttg agaacgtccg
    16861 aggattgctc aatcacgaag gaggggctac gttcgagacg atcctccgaa cgctggatgg
    16921 attggggtat gacgtggagt ggtaggtgct taactcaaag gcctacattc cccaaagccg
    16981 ggagcggata ttccttatcg gacattctcg agacgcatgt accgaacaag tatttcctat
    17041 cattggaccg tcttcaacat ctgatcaaaa catcagaaat ttggtaaata tcaatccttc
    17101 caatcgagga atgggtggac aagtctatgg ttcagatggc gtagcgccaa ccttaacagg
    17161 tgatgaagga atcaaaatcg cactgccagc aagggatgga atctctgtag caggactatt
    17221 accgggaaag tttgaacaag gtaatcgagt ttatgaagtt actggaaccg cccccacctt
    17281 gtcaacgaaa caaggtggta caaaaatcat gattcgaaaa aatgaaacag aatatcaaga
    17341 ggtgaagcca gaagacagtg tgaatctggc ttttcctaat tccacaagtc gccgaggtcg
    17401 tctgggaaaa cagagtgttc ataccttact aacaggagac caacaagcga tcgtcacgga
    17461 tcagtatcaa attcggaagc taacacctag agagtgttgg cgacttcagg gctttcccga
    17521 ttgggctttt gatcgagatt ctcaagtcaa ttcagatagt cagttgtata agcaagccgg
    17581 aaactctgta acagttccgg tcatttttga tattgccaga agattaaaag agttggatgt
    17641 acatggttta tgatgagttg attggagaaa tttattgggt gattggaaag attcagtcag
    17701 acccagaatt agaagaacaa ttgcgacggt taaaatttga tattcgcaag aatggtgtca
    17761 aagtcgctgg cgatccatat ttaatgaatg aggagacgga tgctcgaatt gaaataaatc
    17821 aagtaattgc agaatttgag cggattgcgg accaagcaaa agaaccagat attcgtcaat
    17881 acttgttaga aatgaaagca gagttggaag taaatggtat tacagatgag taacctaaac
    17941 caacacaact gactgttagg cgctgaccgc ggaggattcg cgaagcagaa atcctccgtg
    18001 gtgctgtgcc tgttcatgga cggcgttagc cgaccatgat gtctcacccc cgtatctaac
    18061 aggggggtac aaaaacacaa aaaataagta agcaaccatt aagaatgctt gttgtatcaa
    18121 agatagtaag taatttgtat aggtgttaac tcatttgaaa aagttaacac cttgaatttg
    18181 gaggtgagaa cttggcacaa cggaatttag attatcggct attaaaagac cgacgcaacg
    18241 aatacggcgt ttcacaaaat aaattggcta cggcttgtgg acttagtcgg ccgtacttaa
    18301 atcagataga aaatggtggc gtaaccgcat ctataaaaac catgaggaaa atttttgacc
    18361 aactggaaag ctttaatccc gatttgcctt tgacgttact gtttgactat gtaaggattc
    18421 gctttcccac aacggatgca cgaaaaatta ttcaagaaat tctccatttg aaatttgagt
    18481 atatgctcca tgaagattat gccttttact cctatcaaga acagtatgtc atgggtgata
    18541 ttgtggtgat gttgtctcat gaagaagata aaggtgtgct tttagaatta aaggggcgag
    18601 gttgccgaca atttgaaact tttttactcg ctcaaaagcg tagctggtac gactttttcg
    18661 aggattgtct aaaggccggt ggcgtgatga aacgcttgga cttggcaatc aatgatcgag
    18721 tggggctatt ggatatacct gagctaacga aaaaatgtca gaaggaagaa tgtatctcct
    18781 tgtttcgtac ctttaaaagt tatcgttccg gggaactttt aaaggctgat gaaaaggacg
    18841 gcatgggcaa taccttatat atcggcagtc tcaagagtga ggtttatttc tgtttgtatg
    18901 aaaaagacta tgaacaatat atcaagttag ggattccatt agaccaaact gagacgaaaa
    18961 atcgttttga gattcgactg aaaaatgacc gtgcttttca tgcgattcaa gatttgttaa
    19021 aaggtcgtag cattgagagc accacgtttt ccattatcaa tcgttacttg cgatttgcgg
    19081 ataaagtaga aggtaaaagg cgaaccaact ggcccttgaa tgaacaatgg gggcgtttta
    19141 ttggacggaa tcggaaagag attcaattaa cttccgagcc aaaaccgtat accattgaac
    19201 gaaccttgaa ttggctaggc cgacaagtcg cccctacgtg gaaaatggct aaggagttgg
    19261 atcgattgaa tcggacaacc tatatccagg atatggtaca gaatgcacgc ttatcagacc
    19321 gacataaaaa gattttggag caacaaagta tggcaattga aaatttgatt atatgaaaag
    19381 aggaatcgtc atgggaattg taaaagatat ttttttggta tttggtggca gtatgcttgg
    19441 tgttactgtg atgtgcttga tgaatgcaag tgcagcagcg gatcgagcaa tggaaaagaa
    19501 aggaaagtga caagatgaat ttcggacaaa attt
//
LOCUS       DASHRQ010000053        19201 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.056, whole genome shotgun sequence.
ACCESSION   DASHRQ010000053 DASHRQ010000000
VERSION     DASHRQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.056"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..335
                     /locus_tag="U2M88_002015"
     CDS             <1..335
                     /locus_tag="U2M88_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEM8374377.1"
                     /translation="KIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQ
                     PLQYLEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVT
                     IFFLSILI"
     gene            346..1761
                     /locus_tag="U2M88_002016"
     CDS             346..1761
                     /locus_tag="U2M88_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEM8374378.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            1779..2348
                     /locus_tag="U2M88_002017"
     CDS             1779..2348
                     /locus_tag="U2M88_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEM8374379.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            2458..4212
                     /locus_tag="U2M88_002018"
     CDS             2458..4212
                     /locus_tag="U2M88_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8374380.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            4172..5938
                     /locus_tag="U2M88_002019"
     CDS             4172..5938
                     /locus_tag="U2M88_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8374381.1"
                     /translation="MQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            6461..6730
                     /gene="rpsN"
                     /locus_tag="U2M88_002020"
     CDS             6461..6730
                     /gene="rpsN"
                     /locus_tag="U2M88_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEM8374382.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            6745..6924
                     /gene="rpmF"
                     /locus_tag="U2M88_002021"
     CDS             6745..6924
                     /gene="rpmF"
                     /locus_tag="U2M88_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEM8374383.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            6956..7333
                     /locus_tag="U2M88_002022"
     CDS             6956..7333
                     /locus_tag="U2M88_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374384.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            7354..7503
                     /gene="rpmG"
                     /locus_tag="U2M88_002023"
     CDS             7354..7503
                     /gene="rpmG"
                     /locus_tag="U2M88_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEM8374385.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            7527..8510
                     /locus_tag="U2M88_002024"
     CDS             7527..8510
                     /locus_tag="U2M88_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEM8374386.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            8540..8662
                     /locus_tag="U2M88_002025"
     CDS             8540..8662
                     /locus_tag="U2M88_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEM8374387.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            8680..10203
                     /locus_tag="U2M88_002026"
     CDS             8680..10203
                     /locus_tag="U2M88_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEM8374388.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            10227..10466
                     /locus_tag="U2M88_002027"
     CDS             10227..10466
                     /locus_tag="U2M88_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8374389.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(10659..11120)
                     /locus_tag="U2M88_002028"
     CDS             complement(10659..11120)
                     /locus_tag="U2M88_002028"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM8374390.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11315..11533)
                     /locus_tag="U2M88_002029"
     CDS             complement(11315..11533)
                     /locus_tag="U2M88_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8374391.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            11651..13267
                     /locus_tag="U2M88_002030"
     CDS             11651..13267
                     /locus_tag="U2M88_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEM8374392.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(13508..14848)
                     /locus_tag="U2M88_002031"
                     /pseudo
     CDS             complement(13508..14848)
                     /locus_tag="U2M88_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(15124..15279)
                     /locus_tag="U2M88_002032"
     CDS             complement(15124..15279)
                     /locus_tag="U2M88_002032"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEM8374393.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(15471..15665)
                     /locus_tag="U2M88_002033"
     CDS             complement(15471..15665)
                     /locus_tag="U2M88_002033"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEM8374394.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(15575..15907)
                     /locus_tag="U2M88_002034"
     CDS             complement(15575..15907)
                     /locus_tag="U2M88_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM8374395.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(16421..16612)
                     /locus_tag="U2M88_002035"
     CDS             complement(16421..16612)
                     /locus_tag="U2M88_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8374396.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(16706..17092)
                     /locus_tag="U2M88_002036"
     CDS             complement(16706..17092)
                     /locus_tag="U2M88_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM8374397.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            17786..18991
                     /locus_tag="U2M88_002037"
     CDS             17786..18991
                     /locus_tag="U2M88_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEM8374398.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            19008..>19201
                     /locus_tag="U2M88_002038"
     CDS             19008..>19201
                     /locus_tag="U2M88_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374399.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEAR"
BASE COUNT         6538 a         2799 c         3596 g         6268 t
ORIGIN      
        1 aaaaaataca tttaccgaaa aaaataatgt taagtattac agtagtgatc agattctttc
       61 cgacttatcg ttctgaaata agaatgatta aagaatcttt aaaaatgaga aacattcgct
      121 tgtctttttt tcagcctctg caatatttgg aatttctgtt agttcccgtt cttatacgtg
      181 caactatgat tgctgaagaa atgaccgcta atgcaataac aaaggggata gaatctcctg
      241 tgaaaagaag ttcttactat aaaacaaaca tgagcttatt agactatatc atcctgtttt
      301 ccgttactat ttttttctta agtattttga tttgaggtga aagaattgtt tgaatttaac
      361 aatgtttctt tttcatataa ggatgttaag gaacaagcgc ttgttgacct taatttttca
      421 ataaaaaaag gcagtcttac attgattact ggttcttctg ggagtgggaa atccacacta
      481 ttaaagttaa tgaatggact tattccaaga ctttatgacg gaaaattggt tggggaaatt
      541 ttatttgaaa ataactcgct acaatcctta acacaagaaa gtatatcaaa aaatattggc
      601 tacgtaagtc aaaatcctag aggacaattc tttacaacaa atacaacgag tgagttagta
      661 ttttcgatgg aaaactttgg atttacaaaa gagcatatgg ataaaagaat caatgaatta
      721 gctgaattat tagatctttc aaatatatta gataaaaaca ttttttcgct atcaagtggt
      781 gaacggcaga aaatttctat tggatgtagt ttatcgctaa actctaaatt aattatattg
      841 gatgaaccgt cttcaaattt ggacttcaaa gcgactaaga aacttgctga cttactttgt
      901 gatttaaaaa ataaaggatt tactattgtt atagctgaac atcgttttta ctatttgaaa
      961 gaattaattg atcaagttct atttgttaaa aatagaactg taaaaactac aactattcag
     1021 ttgttaaaaa ctgaaaacaa taacgcatta aggaaatttg acatttttaa tagttcgatt
     1081 gaagttccct atatcgaaaa ggtaagtgca gatgtattaa aagttgaaga aattacttat
     1141 gaaaatattc taaaagacat aaattttgat gtcaaagttg gagatgttat tggtttggta
     1201 gggaaaaacg gtgtggggaa aacaacctta ctaagaatac tcatgaagat tattcgccct
     1261 accaaaggaa agataatcga aaataaaaaa aatttgtcat cgccgttttt agtaatgcaa
     1321 gaaatggatt accaattttt cacagaatca gtgaggagcg agttagccct ggggaatgaa
     1381 atagttagta aagatcagca agaaaaaatt ttaaaaaaaa tggaacttta taaaatgaaa
     1441 gatcaattac cgtttgattt atccggtgga gaaaaacaaa gattattggt ttctatagct
     1501 tcattgtcaa aaactaattt atttctattt gatgaaccta caagtgggtt ggattatatc
     1561 aatatggaaa ggattgctga ccttattaaa gacttaaaag aaaagggtgc agtagttata
     1621 gccactcatg atccggaatt tctttataaa acatgcaata gaattattta tttaaaaaat
     1681 ggtggaataa aaaaagatat aaaacttaga gtagaagatt caactttgat agaacagatt
     1741 tttaatgaca taacgaaata gaattgagga gaatatatat gaaaacaaaa gattatattt
     1801 ttattggttt agctacagtt atttccctag taatttactt tttttcaata atggttagta
     1861 gtattggtgg ggcgtttggt cacagcatat ccccagggat ttttggatta ttatctggaa
     1921 tcatatttgt ttatattagc tataactacc ctagaaaagg aatttttact atttacacaa
     1981 ttgttttatt gttatttttt acgttaatgg gcggggcgta cttaccttgg tttatcagtt
     2041 cgattagtat ggcgatctta gctgatatta ttctatctgt atttggatat gatcgagcaa
     2101 ttcctcaagt tgtgtcttgg gttctaatgc aactaggtag tgctgccgga cagtggattc
     2161 ccatatggtt ctttacagat cgttttcgtc aagactggat agatagagga caatctgctg
     2221 caacaatgga tgcaatgatc cactacgcgg tgggtatatg gggaattatt tcagtgttag
     2281 ttgtagcaag tttatctatg attggtgtat tgattggaag aaaagtctta aaaaaataca
     2341 aaaaatagaa cgtagtttac cttctatctg tttgtacatc tctaatttac gctattacgg
     2401 aaagcattaa cagttgtaaa cgaataagag aagaaattga atgcggggga ggaaaaaatg
     2461 aaagtataca aaaaattatt tagttatgtc cctaaaatga aatactacgg atggttagcc
     2521 actttatttt ctgatttttc tgtcgtatta acagtctatg gatattacag tatttacaaa
     2581 tttcttcagt ctctaattat tgtaggagat gaatatttag ctaagagtta tgcggttcaa
     2641 actgttgctt ggttaacttt aggagcttta ttttatattt tttctggctt attttctcat
     2701 atccttgggt ttcgcttaga gactaatttg agaaaaaagg gaattagcgg tttaacagaa
     2761 gcaagttttc gtttttttga tttaaattct tcgggatata ttcgaaagac aattgatgac
     2821 aacgcggcta aaacacatat ggcaattgct cacttgattc cagatagtgc tcaagccata
     2881 gtaacacctg ttttatcagt tgccttaggc tttttcatta actttaaaat tggattggtt
     2941 ctaatcgcca tgttagtaat tggtgtattc ttgttaaaga aaatgacaga gaatacaaat
     3001 tttattcaaa aatatcaaga atcgttagat gtgttaagct ctgaaacggt agaatatatc
     3061 cgcggaattc aagttataaa gatatttggt gctaaagtaa catcatttaa agccttgaat
     3121 aaagcaatta acgattatgc taagtttgca tataagtatt ctttgagtgg gaaaacacct
     3181 tttgttcttt ttcaatggct attttttggc atagtttgca ttttgatagt tcctataacc
     3241 ttttttataa cttctcttac cactcccaag atcttagctg ttgacttgtt gatgttgttt
     3301 tttttaagtg gaattatttt tgtttctttc atgagaataa tgtatgtaag tatgcacttg
     3361 tttgaagcaa gatatgcagt cgaaacacta gactcccttt atttagaaat gaagcaagac
     3421 caactaaaat atgggaacga gacacagatt aaaaattgtg acattgcatt tgaaaatgtc
     3481 agcttttcat acaaagaaga gtgtgtttta gaaaatttca acttagtttt agaacaaaaa
     3541 agaatttatg cattagtagg aaaatcagga agcggaaaga gtacgatagc taaactaatt
     3601 tcaggtttct acaatgtgga taagggacag attaaaattg gtggtaagcc tttaaatcac
     3661 tattcaaaag acacaattat taacacaatt gcttttgtct ttcaagatac gaaactattc
     3721 aatcagagta tttacgataa cgtttctata gcaaatcctc aagcgccagc aagtaaagta
     3781 tttgacgcgc taagattagc cggttgcgaa tcgattttag aaaaatttcc cgaaagagag
     3841 cataccttaa ttggtagtaa aggagtttat ctgtcaggag gagaaaaaca acgtatagct
     3901 attgccagag caattttaaa agatgcaaag attgttatta tggatgaagc cagtgcatct
     3961 attgatccag ataatgagta tgaactacaa aaggcttttc aatatttgat gaaagaaaaa
     4021 acagttatta tgatagctca tcgattatcc agcattaaat ctgtagatga aattcttgtc
     4081 ttgtcagaag gaaaaatagt agaacgtgga aatcataatg aattaatggc taaacatcga
     4141 gtgtataaac aattggtcac taattatgag aatgcaaatg attggaggct tgacaatgaa
     4201 aaatttttat aaaaaaaaat ttgctttaac tgatcagggc gcagaagctt taactaaagc
     4261 ttctatttct agtttttttg tatactgcat taatatggta ccagcattca ttataatgat
     4321 gctgatagac gaattagttt tagaaaatgc aaagccccgt tggctttatt ttgcagtatc
     4381 gtttgttact ttgcttttca tgtattggtt gttagatagg gagtatgaaa acttatataa
     4441 tagtacttat aaagaaagtg cgcatttaag agtgcaaatt gcagacgatt tgtcaaattt
     4501 accattatcc tatttttcaa aacataattt atcagattta tctcaaacta tcatgtctga
     4561 cgttgaaggt attgagcatg cgatgagtca tgcaatacct aaagccggcg gtatggctct
     4621 gtttttccct tttatttcag tgatgctttt ggttggtaat gtcaaaatgg gattagctgt
     4681 tattttgcca acgttattta gttttgtctt aatcttgtta tcaaagaaat cccaaacgaa
     4741 agccaatact aaatattacg atactttgag agaaaactcg gaagaatttc aagaaactat
     4801 tgaattgcag caagagatta atagctttaa tctatctaaa aaagttcaag acagactttt
     4861 caaaaaaatg gaagagagtg aaaggattca tttaaaggta gaattaagta ctttttcagt
     4921 catggcctta tcctctattt tctcatatgt tagtttagca gtagtcattc tagtaggtgt
     4981 tcacttactg ttaacgggtg aagtgactat actctacgtc gttggttact tactagccgc
     5041 aataaaaata aaggattcct tcgattctat gaaagaagca gtgcttgaaa tattttattt
     5101 agctcctaaa atacagcgaa ttagagctat gaaggaaacc agtatccaag agggatcaga
     5161 tagtcctctg aaatcttttg acattgagtt gcgagatgtc tctttctcat atgataacaa
     5221 taccccaatt ttagatcata tctcttttac ggctaaacaa ggagaagtga ctgcgttagt
     5281 aggtgctagt ggttccggga aaactagtat cttgaaatta gtgtctagac tgtatgatta
     5341 tgatgaaggc tgtattttaa ttgatgggta tgatattaaa cgtgtatctc cagcttcttt
     5401 gtttagcaaa atcgctattg tttttcaaga ggtaactttg tttaatacaa gtattttgga
     5461 aaatatacgt attggaaata gtcaagcgtc agatgaggaa gtgaaaaaag cagctcgttt
     5521 agctaactgc gaagacttta ttgaaaaact acctgacggt tatcatacat tggtcggaga
     5581 aaacggaagt tcactttctg gaggagagcg acaacgccta tccatagcac gggctttttt
     5641 gaaaaatgcg ccaattttaa ttttagatga aattactgcc agtttagatg cggaaaatga
     5701 aaaacgtatt caggaaagtt taaatcgttt aatacaagat aagacagtac taattatatc
     5761 gcatcgcttg aagtctattg aaaaggtaaa taaaattgta gtgatggatc aaggaaaact
     5821 agttgatcaa ggtactcata atgagttgta tcgaaggtca gaaatataca aaaatctcat
     5881 aaagaaaact aaactatctg agaaattcgt ctatgaaaaa gaagctcaat cacgatgatg
     5941 aaataggctt taaattaacc tacaattttt gggatatagt tataattctt tttcaaaaag
     6001 aataatttta ttaaacagca tctaattttg aatacacgaa agaatcagta tgattgaatt
     6061 agttcactta atgtctattc ttataatata aggaataatc aaagttttaa aaaattggcg
     6121 ataaaccagt taaacataaa agattgaata aaaagttata gtttctttca tattgccgtt
     6181 ttaagtagag gaaaagctac ttaaaacggt atttgtgttt gattggacta ataaaagtga
     6241 attcttattt ttcagagcct aaactgcata ttcttttaaa tgatttgatt atctctatgt
     6301 accgataaat tcgttttcaa atagttatct attcatatga gttttatttc atgttattta
     6361 ttgttgacaa gagcaactct tgttggtaaa gtatcagtgt caaaacgtaa cgattacgat
     6421 ttgaggtgtc taaatcaaaa taaaaggagt ataaaggtat atggcaaaaa agtcgaaaat
     6481 tgctaaagca aagaaacaaa tggcgatgat tgaaaagtat gcagataaac gtcaggaatt
     6541 aaaagccgca ggagatcgca ccgctctcgc taaattgcca agagattcaa atccgaatcg
     6601 tctgaggctt cgtgatcaga cggatggtcg tccaagaggg tatatgcgaa agttcggtat
     6661 gtcccgtatt aagtttagag aacttgccca tcaagggttg attcctggag taaaaaaagc
     6721 aagttggtaa aaggaggaaa tatcatggca gtaccagcaa gaaaaacatc taaggcgaag
     6781 aaacggttaa gaagaactca tcaaactgta gtaaaaccag aaatttcatt tgacgaagcg
     6841 aatggtgatt atagacgtag ccatcatgta tctttaaaag ggtactataa tggaaagaaa
     6901 gtaattaaag gagcttcaaa atagtttaat atataacttt taggagggaa tcaaaatgga
     6961 aattatttac ccacctttag ttgaagaagg attaaagtat tatcttgaaa cgacccagca
     7021 atcgctagat aaatgcactt tttatcgttc gatggtagag agaggaatta ttactgaaac
     7081 gggtttaccg acccaacagg caattgaaaa tggattagtc aaagattact atgaggacca
     7141 aggcttatca tttgatgatt ttttaagaat ctatccaatt tttgaagagt atgacgagga
     7201 gttatttcaa tgtatcgatg gttattggga gatacctgtt gatatgaaag aaaatttagt
     7261 ttcacaattg gaatctggcg agttaacttt tgaagatgca caacaaattc aagcatatct
     7321 tgaagatcga taagtattga aggaggaaga tttatgcgtc aaaatattat tttggaatgt
     7381 gtcgaaactg gagaacgtct gtatctcacc agcaaaaata aacggaataa ccctgaacgg
     7441 ttagaattaa aaaagtattc gccaaaattg cgcagacggg caatttttaa agaagtgaaa
     7501 tagctaagtg gtaaaaaggt gacaaaatgg gcataccagt tacaatcgtt tctgggtttt
     7561 taggggctgg aaaaacaacg ctaatcaatc aagccttgca agtctcaccc taccccaggg
     7621 aagaaattat tataattgaa aatgagtttg gtgaagttgg gattgatcac aaattattag
     7681 ttcatagcga ggaacgggtg ttacaactaa ataatggatg tatgtgttgc agtcttcgca
     7741 gtgatttatt agcaatattg atttctttac tggaaacaat agaagaacat caacaaccaa
     7801 tttcacaaat catcattgaa actacaggta tagcagatcc ccaaccaatc attcaaacac
     7861 ttttaacagc gcctcaacta agaggaagag tatatatcga tagtttattg actgttattg
     7921 atagtgacag ttttgaactt attgaaagag aaccacaagc ccttaagcaa cttgtcatgg
     7981 cagatcgcat ttttgtgtct agaaaacaaa acttgaattt atctgcacta aaaaaattac
     8041 agagagaaat caaaagtatt aaccctttat ccgatattcg aatgttcaca catagtgaaa
     8101 cgattcaaaa aaaggatttt tataatttga aaaaatttaa tcatccctta gtttttgaaa
     8161 gtgattcatc agaaaatcac tataatggca aatactatca tcaacaccat caagtacatg
     8221 agtttaaatc aattctctta acttcagatc aagacataaa aaaagatttt ttactccaat
     8281 ggattgactg gctgatttta accaaccaag ggagcctgta tcgttttaaa ggattgattg
     8341 ctttacaaga gcaggatttt atagttgcca tgcaagaggt aaacgaacag gttaattttc
     8401 aatacaccac tcgacaaaaa gaccaaacca ctattattct aatcggaaaa atgttggatg
     8461 aagaaaggat caaacagagt tttgataaat tactggcgga aagcaaataa tgataagtag
     8521 tgattggggg taatttctaa tggagaaaac ggatatatct agtgcatatc gacgacttaa
     8581 aagcccgaat attaaaacta gaaaaagggc attaaaaatt atcaaagaac ataaacgaaa
     8641 taaaatgaag aaattagcat aattaaggaa ggaaaaagaa tgaagaaaat cagtgtagga
     8701 cttattggga tagcagctct aggattattg ggagcttgct ccagcactaa tgatgcaaaa
     8761 gtatcgaatg ataaagatgg aaaattagaa attgtaacaa cattctatcc aatgtatgat
     8821 ttcaccaaaa acatcgtcgg cgatgaagcg aacgtggatt taatggttcc agcgggatca
     8881 gaacctcatg actatgagcc ctcagctaag gatatggcaa aagctcatga tgctgatgtt
     8941 tttgtatatc ataacgaaaa catggaatca tgggtaccaa aagctaaaga aagttggaag
     9001 aaagctgggc ctaatgtagt agaaggaaca aaagacatga ttttattgcc aggtagtgaa
     9061 gaagaagatc atgatcacgg agaagaagat caccatcatg aactagatcc tcatacgtgg
     9121 gtttcaccca aaatggcaat taaggaagtt tcaaatatca aggatcagtt agttaaattg
     9181 tatccgaaaa aagcaaaagt atttgagaca aatgctgaaa agtatttgac taaactgaaa
     9241 cggttggatg ctgactatac cactagtctt aaagaagcga aacaaaaaag ttttgtgacg
     9301 caacacgcag cctttggata tttagctttg gattatggat tgaaacaagt tcctattgct
     9361 ggactaagtc ctgaagaaga gccatcttct ggtcgattag ctgaattaaa agagtatgtt
     9421 aaaaaaaata agatcaatta tatttacttt gaaaagaatg ctaatgataa aattgcaaaa
     9481 actttggcca acgaagctgg gataaaattg gaagtactga atcctcttga aagtcttaca
     9541 aaagaacaaa tggataatgg tgaagattat gtttcagtaa tggaagacaa tctaaaagcg
     9601 ttagaaaaaa caactatggt tgctggtgaa gaggtagttc cagaaaaaga agcgaaggat
     9661 gaaaagacag ttgctaatgg gtactttaaa gatgccgatg tgaaggatcg cgaattgagt
     9721 gattatacag gcgaatggca atctgtttat ccgttgttaa aagatggaat tttggatgaa
     9781 gttttcgatt acaaagcaaa aatcaataaa gacatgacag ctgccgagta caaagattat
     9841 tatactactg gctataagac agatattgat actatcaaca ttaaagacaa caccattgat
     9901 tttgtagtca acggtgaaca tcatcaatac acatacaaat atgttggata caaaattttg
     9961 aattatgaaa aaggaaaccg tggtgttcgt tttaattttg aaactgatga tgcaggtgct
    10021 ggtcgtttta aatatattca atttagtgat catggaattg caccaagtaa ggcagagcat
    10081 ttccatattt tctttggtgg agaaagtcaa gaaaaactct ataatgaatt gcataattgg
    10141 cctactttct atccagcttc attgtctaaa catgaaattg ctcaagaaat gatggctcat
    10201 taaaattcaa tttttggagg aaatacatgg taagaaaagt attattgtct atggattcca
    10261 agaatattga tgcttatcgg cgttatcgat ttgcaaagca aatgagatta aatgggagta
    10321 cattagctga tattgctgca actttacatg ttagtattga cacagttcat agagtattaa
    10381 aacgggatcc tgattgttgt tttatcaagg tcaatactgg aagaagtaga cgtttagcaa
    10441 aacggcataa tagcttggcc aattaattga taataccaga gtagatcatt tttcccttat
    10501 ttggatttga tctgcttttt attatgtctt gaattgtcaa atcaagcgca acactttatc
    10561 aaagaagact tcataaggtg ttctccaatc taaacacttt ctaggtctgt tattcagttt
    10621 ttcaatcatc tgatttatcg ttgagttatc taccgtagct atctctttcg attttggcaa
    10681 gtattctcga atgagtctat tcgtatttta atttgttcat tgttgccaag ggcatgagga
    10741 tctggaaaat aaaagggcat attattcaat gcttctgtaa ctgcgaaatg attggagaat
    10801 tcttttcctc tatctggagt gatcgttttg accgcctcat ttggaatact gcttaaaagt
    10861 tcaatcattt tctcgctcac ataagcggct ttctttctgg ggaccttctt tcctgctaaa
    10921 aggaagcatg attttctgtc tgtcaaagtc actaaacaag taccaccagt tttgccagct
    10981 acaatatcgg ctttccaatg accaaaatca cttctatcat ctgcggattt tggacggcca
    11041 tggatcgaat gagtaaggca aatctttcca cgagactcga catgattttt tgtatgtctt
    11101 gtttttcctc gatggtgcaa tgaacggatc aggcctttat tgccgtgtga gaggggttct
    11161 gtatcgaaaa gatcattgca aattgcacgt tagatcgtga taaaactaat tgaaaagtcc
    11221 atatgttcat gcttcaaacg attcgagatt tcctctggcg accactgaaa atcgacaaat
    11281 aaatgacgaa caattgacca aactcacgga tcatttagaa tgtgataacg tccgcatttt
    11341 tttcttcgat aagcataatt tttttccgct ttggaaggtg aatagttttt ttcttgtttc
    11401 ttctcagttc atgtaaaata gtaaacgggc tatgctgaat gtcgttgcca attatactaa
    11461 tagaaaatcc ttcttcgtga agcagaaaga ttctttctcg ttcgagtata gtaagatgac
    11521 gatactggct cataattatt ttccttctag gagtgttctc gacaaactta tactagttgg
    11581 atatcgatta tgactcattt ttatgtgttg cacttaaagt gtaaattcaa ggtatagaaa
    11641 gaggttccct atgaaaaaaa tacctattac aatcttaact ggctatcttg gttcaggaaa
    11701 aacgatactt ctaaatcata ttctgtacgg tgatcacgga aaggaaattg ctgtcattgt
    11761 aaatgacata ggggaagtaa atgttgatgg ggtattagtc gacaagaaag gttttaaaag
    11821 aactggagaa aaacttgttt cgatgccgaa tggatgcatt tgttgtacat tacgcgaaga
    11881 tttgattgtt gaactagatc aattagtgca attttctgac ttgaatcaaa ttgttattga
    11941 agcttcagga atcagcaaac caattccaat tattcaagca attatattag ctaaaaaccc
    12001 tttagacatt gatttgaccc aacgattaga gattggtgcc attattatag tagtggatgt
    12061 aaaacgtttt tgggataatt tcgaaacggg tgaaaagatt caggaaagaa aagctgaagg
    12121 taatgtagtt gaagaagaac aggatattcg tggcttgtta attgatcaat tcgagtgttg
    12181 taacatatta cttttaaata atagtgactt agtgtcaacc aagacgattt acaaaattaa
    12241 cggtctaatt gctaagttac agtccaatgc caaaataatt aggacaatta aaggtgaagt
    12301 agacttatcg aaaattctaa atacaaatct ttttaatata gatgagactt cctattcagt
    12361 gggctggttc aaagagttag agcttggcta tcaaaatcat attcctgaaa cagaagaata
    12421 tggtatctca agttttatct atcgaaatcg gttactcctt gacgcacaaa aatttgccca
    12481 tcttacggat aaaaattttc ctggcaatat tacaagagca aaagggtttc tttggtttgc
    12541 ccaacatagt gatttttcga cgatctttga aacatcgggt agctataaaa gtattgaatc
    12601 attaaattat tgggtagcta gtttacctaa aattgagtgt caaatgatta tacaaaaaaa
    12661 tcccgaattg aaggaagcct ggcatccgtt gtttggtgaa cgcattaata aactagtttt
    12721 tattggtatg aagatggatc aacaatgtat taaggaacaa cttgagggtt gtttaactga
    12781 ttctcttaat gcagataatt tccgaaaaat ttcaccattt ccatggaaaa agtctaatat
    12841 gtttagccag atatcgttaa tgaaattttt aagaaaagct ggtgtcaaga taggcgatgt
    12901 cataattatt ggcattttga ttattacctc ttttattccc ttatttgtat tctctataga
    12961 aaaggagaat gaaacaactg aagatgctat caaatatgcc atcgttcgaa ttgatggcaa
    13021 ggaaatggat cgcttcgatt tggataagat caatcataaa ctcgtgaatt atcatcctgc
    13081 gaagggacaa tataatattg ttgagattaa agatggaaga attcgagtta aagaagataa
    13141 tagtcctgat caaatcgcgg ttagaactgg atggatctca aaacccggtc aaacgagtat
    13201 ttgtttaccc cataagttag ttattagtat tgagaaaaaa gaaagcacag attactacat
    13261 ttactaatct ggaaatagat gaggaggttt ttaataagaa aaagatatta ttattaataa
    13321 tagcaggggc tttattaagt gtgttaagcg cttgtggaca agaaaaagaa gcggtgacaa
    13381 caggcacaag tatctttaac gggacctgca atggtttagt taacagtcgg ttagattact
    13441 tattttggct cttcgtcaat gagtgttggt gaagaaattc aatagaataa tcgagatact
    13501 cacacggcta gacagctatc ttgtctagcc gtgttgttgt tctggcttta gtcagttggg
    13561 tagtattctt agttttctta aaatattttc ctcgatataa aaagattcgt tctcggtagt
    13621 tttgaaagtt tctatagcca taggcaattc gttttagtac tttgatatgg ttattcaaac
    13681 attctatagg gccgttggaa tagggaacgc gcaacgctcg tttgatttct gtttggtatt
    13741 tcttgaaggt cgtgaacact ggttggtagt gctctggcaa ctccttgtag tcttctttta
    13801 ggagcgcgac gaattcagga acatcttgtc ttctgataac ggaaagaaaa tcctggtaga
    13861 cttcgtagcc tcgctttaag gaatcatcat aagataatag ccgatcgact atctctgctt
    13921 ctgtcagatg atctcgaaat gacggacgcc agtagcgatg ctgacctttc aattcccacg
    13981 cattcttttg aagtagtttc cagtatttct ttaagtgctt cgaccgacga tccccttttt
    14041 taaaggtctt cattacttga attcggtggt tggtaaaagc ccgaccaatg tgttggctaa
    14101 tatgaaagcg atcggcgacg attcgggcat tcggaaaaca ctctttcacc aatgtgcgat
    14161 agggtgtata gaaatctgaa accactagac gcacttgttc acggactttt atagggtagc
    14221 gcaagaaata gtcgcgtaaa ggattgagtt gccgattttc cacaatatca atcagttgat
    14281 gggtcttgcc gtccatcatg acaaagctca tggctcccga gacatttttc actgatttga
    14341 attcatcgaa acacaccact tctggtaaca gctggctcaa agaaatggtt ttaggccgaa
    14401 ggctgcgaag tattcgaatg acagtggtcg gagaaatgtg cttcatacgt gcgatcaaag
    14461 acatagaaag aggttcgctt aatagctcaa gaatggcttg tttgactctg cgagcaatgg
    14521 agcagcgacg atcactaact gaatcttctg ctaaaaaagt ctttccacag tgtttacatt
    14581 tgaagcgctg tttgttaatc cgcagaatgg tcttgtagtt actgacatca ttcaataaga
    14641 tggatacttt tttccagccc tatttaatga tcgtttgttc attttgatag tggcgatgaa
    14701 agcattcctc cggttgatag gtcaatgtgc catcaaaagc aaaacatgtt tcgtcattga
    14761 tgcgctcttt gcgaaaggca tcctcatgaa aggtaagatt taggtctaga atatctagta
    14821 actctttggt aaactctgaa taggacatct tgaaacacta ctttgaatgg tttttgtcga
    14881 ctttattcta aaggatttta aggtgtctgt ttgcgtttat tagaaaaaag aaaaagtatt
    14941 ggcaacagaa ggcgtcacca acactagata ttatagatcc cttattttct tcgcttcaaa
    15001 ttgtcaagga gtcatcataa attttgtatg gattcttttg tttgggtagt agtcttctaa
    15061 ataatcttgg attaacacct tcatctctat tttactttta tatttgttgg agtcgattaa
    15121 ttctcatttg attgatcgat gaaacgcttc aattactgca ttgtcaacag gagtcccagt
    15181 ttggctcact gaatgtgtga tgtggtggtc gttcaacagc tcttgataaa taaaagaccg
    15241 atgctggcta cctctatcgg aatgaattac cgtaggcaat tctccttaat aaatttgtaa
    15301 tggacggacg acaatctctt tttttatgtg tgaatctacc atatagtaac gtagaatacg
    15361 agttgctaag tcaatatagg tggacaaata gaggcgaccg tcattacatg gaatgtaagt
    15421 aatatccatc acccaaacag catcaatttg gttctgttca aattatcgat tcaacatttt
    15481 tttaccattt ctttgattgt tttttctcta ccatgagaag atttgcgtcg atgaaatcct
    15541 cttgtgcata aattcatttc acgcataaga acagctacaa cacgtttatt gatttcgatt
    15601 ccttcatcgt aaagtttttg agtgatccct ggagatccgt atcgatcttt gtgttcgtag
    15661 aacactcgtc ttcctctttt agtcgtttat tttcctgttc aagaatgtag aaatctgctt
    15721 tatcaggtaa tctttttcct tgtccagcaa aagcattttt cccgtatcga cgatattctg
    15781 aaacccatcg ggaaattgtt ggacgagaga tattgtattc ttcagcaatt ttcttggtag
    15841 ttactttttt ctcaaggata tcctgtgaaa tctttagttt aaatttacga tcatatgttt
    15901 ttctcaatag agttcattcc ttccggtaac gtaactgata gtcaagaaaa gtgtatcata
    15961 gcccaactta gtatcagggt ttgtcaacta aactgtggaa gttgatagat aagagttttt
    16021 aacaaccaaa attctcttga ctatttttga aatcagacaa attttgattg gacacatagt
    16081 tgaataaatc taccgtcgtt acggaaggta aatcgcatgc ttttcaattc tagaggagaa
    16141 gtcctcgttg aagtaccata gcccagttct ggattcgacc tcctgcccac ttcatacgta
    16201 aataaagtaa ttttaggagt gagactgtga caaaagttat ttttaagcta aaaacgaatt
    16261 aaaaaacgtt gataattaaa atcattcgtt ttagattcta tgaatcatta tagaacgctg
    16321 taaaatcgat actttataat tataaagaag atagtttcaa atttattgtt cgaaatttga
    16381 aactatcttt ttacttatgt cacagactcc cttagttgag ttattcttta atttcgaatt
    16441 tcgagatagc tagatatatt gcaagcatac attcttgata acaatcttga tcatattttc
    16501 cagttctcca ggattgctta tgaatcaaag agctaaatct tttgagaatt gcctcaacag
    16561 cttcttcatt tccagccttt gctaaaatga aatcttcttt taaagaattc ataaatctac
    16621 cttcttccca tggacttata atctatttct ttgggaagga ggagaacgag caaagaagca
    16681 tacaaaatta gttattatgc agatattaaa ccagcatttt ttgaattttt ttgtaaagtc
    16741 gatgttttaa atttgtcgca ccctgtcttg taatccccag aacctttcca atttctttat
    16801 cagttttatc aaagataaat ttttcgataa gaaaaatttg ttcaggttct ttcaacaaag
    16861 gaaggatttt tgctaatttt atatcttcaa caaggagagt cacatttaaa acattaatcg
    16921 gttgtttaaa ttttatagta tctttagtaa acaaatatgg tgggagttga tcaagttgtt
    16981 catgtttttg aatttcttct tgttttttat aataagtatt agcggtgttc aaaatgacct
    17041 tttcaaagaa tcgaatcaac tgggcattta cgcgttcgtc gttatctgac atattgcaca
    17101 cctcctccat taagatttaa agggaaaaga ggcaaaaaac acgaaaagaa gctaatgcaa
    17161 ttatgctgcg ctgcgaataa cttctataag ctcacatgca ttgataggac tgctgatgtt
    17221 tattttcaaa aatgtttttt tagttgataa tggatagtta tctttatact tagaaaagaa
    17281 aattcatata tcttcttaaa tatcaaacat gtccttatgg ctaagaaaat gtcttgagct
    17341 ttttacgtat aataaatctt ttctaaaacc tccttttgga taatattctt attcgtgtat
    17401 agattgtatt ttttatttct tcccccttca taattaacaa ctagaaaaga gttcgaatag
    17461 acacgtattt gaacgaaaat ttttattgga ttcccaaatt ggaatattta gaaacgaaaa
    17521 attcctatta tctgtatagt gcttagataa taacggatca taataattat tgtctttgaa
    17581 ctagatatat gtaaatagta ttcatcatga tgaaaaatga tgagttctcg gtgataaaat
    17641 gtacttttta cggtggaata tacatgaaag cgataagtgg atattttata atgaagtaag
    17701 ttagcttatt ttgacagagg aatagtattt tagataacgt ataaaatact atttaatgat
    17761 gtagaaagga aagagagaaa caaaagtgtt ttcagataaa gaagtcttat taaagtctct
    17821 tcatgtttgt ttaaagttac ctatcgtggt ttttaattcc gaattcagta ttataaaaga
    17881 gtatcgctca gatagaacta cttatttttt ttatgattat aaaaagttac taactgataa
    17941 tattaaaaaa actaaagatt tccatttctt tactggtgat tttaatgaga tgtttcttct
    18001 atacatgttt aaaaccagat attacttatt tggtccattt agagcaaata atattgataa
    18061 agtttttttt aaactaaaaa tgaacaattt gaatgttgca atggctgata gagaacgact
    18121 ctataattca ttgcaaaatt tgactttgta ttctttagga gacattcgag atattctgat
    18181 tttagttcat tattttttta ctggaaaaat agaagatcta ttccatgaac cgttaattga
    18241 atatacagaa aatctaagta aaactatcga gcaaatcaaa atagataatc tgttatcaca
    18301 aaattacgat ccagaaatat atctgtttct ttatgaaaat aaaatactgg aatatgttaa
    18361 aaatggagat attcgaaatt tagaaaacat ggtttttaat ctgagtaatg gaataattcc
    18421 aagtgttagt ggagatacga ttcggtctga aaaaaattat tctatcattg tttttgaaaa
    18481 gttagcacaa acgtctataa cattaggaat ggatataata gaagcgtatc aaagtagaga
    18541 tgctttgata caagaaaacg aattagctgt atcgttacct gaggtcttaa aagtgaggga
    18601 ttctgggatt gtttattata ctaaggaaat cggaaaaact aagatagagc atctttctcc
    18661 cttaatttct tctgtcgttc aattcatcgg cttaaatatc tataaaagaa ttactgtaaa
    18721 ggaaatagct aattattttt ctgtaagtga aactaaatta agagaaagct ttaagaatga
    18781 aatgcatatt acgatttata attatatctc taagcgaaaa atttctacgg ctaaaataat
    18841 gttgaaatca aaccatacta tcagtgaagt ttctttaggt ttaggatttt cagattcttc
    18901 acacttttcg agagtattca aaaagtacgc aggtgtatct cctaagcaat atcaattagg
    18961 tttagtggac aatttcaaca acattagctg agaggtttta ttttattatg agactatatc
    19021 atttacgtag tttggaggag ttgaggaaat tggattttaa caaaaaattt ttttgggaaa
    19081 tttctgattt gagttgtttc gtagaatctg agcgtctaag taatggtaat aatagtgtgt
    19141 taaatgaata tgacaaagta gtaattaata aagtaaaaag tttatgtgaa gaagctagag
    19201 a
//
LOCUS       DASHRQ010000054        17175 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.057, whole genome shotgun sequence.
ACCESSION   DASHRQ010000054 DASHRQ010000000
VERSION     DASHRQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.057"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            7..1398
                     /locus_tag="U2M88_002039"
     CDS             7..1398
                     /locus_tag="U2M88_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEM8374400.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            1414..2454
                     /locus_tag="U2M88_002040"
     CDS             1414..2454
                     /locus_tag="U2M88_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM8374401.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2473..3558
                     /locus_tag="U2M88_002041"
     CDS             2473..3558
                     /locus_tag="U2M88_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEM8374402.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            3591..4553
                     /locus_tag="U2M88_002042"
     CDS             3591..4553
                     /locus_tag="U2M88_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEM8374403.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            4546..5979
                     /locus_tag="U2M88_002043"
     CDS             4546..5979
                     /locus_tag="U2M88_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEM8374404.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKGELI"
     gene            5976..7046
                     /locus_tag="U2M88_002044"
     CDS             5976..7046
                     /locus_tag="U2M88_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374405.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            7033..8454
                     /locus_tag="U2M88_002045"
     CDS             7033..8454
                     /locus_tag="U2M88_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEM8374406.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFITIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            8467..9474
                     /locus_tag="U2M88_002046"
     CDS             8467..9474
                     /locus_tag="U2M88_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEM8374407.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            9486..10490
                     /gene="neuB"
                     /locus_tag="U2M88_002047"
     CDS             9486..10490
                     /gene="neuB"
                     /locus_tag="U2M88_002047"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEM8374408.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            10487..11635
                     /gene="neuC"
                     /locus_tag="U2M88_002048"
     CDS             10487..11635
                     /gene="neuC"
                     /locus_tag="U2M88_002048"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEM8374409.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            11608..12285
                     /locus_tag="U2M88_002049"
     CDS             11608..12285
                     /locus_tag="U2M88_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEM8374410.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            12275..13417
                     /locus_tag="U2M88_002050"
     CDS             12275..13417
                     /locus_tag="U2M88_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEM8374411.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            13430..14407
                     /locus_tag="U2M88_002051"
     CDS             13430..14407
                     /locus_tag="U2M88_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEM8374412.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            14407..15315
                     /locus_tag="U2M88_002052"
     CDS             14407..15315
                     /locus_tag="U2M88_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEM8374413.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            15316..16068
                     /locus_tag="U2M88_002053"
     CDS             15316..16068
                     /locus_tag="U2M88_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEM8374414.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            16073..17020
                     /locus_tag="U2M88_002054"
     CDS             16073..17020
                     /locus_tag="U2M88_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEM8374415.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         6193 a         2196 c         3106 g         5680 t
ORIGIN      
        1 gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg
       61 gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag
      121 atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt
      181 attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc
      241 gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt
      301 gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg
      361 cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat
      421 aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt
      481 tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac
      541 atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact
      601 gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca
      661 acatttgaaa acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt
      721 attgaaacat ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat
      781 atcatcgttt cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt
      841 ttagtcaaag tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat
      901 caaaaggaat tttcaatcta taaattccgc tcgatgaccg ccacagccga agcaaaatct
      961 ggaccagttt tagctaagag caatgatgca cgagtaacgc ctgtaggtaa atttattcga
     1021 gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc
     1081 gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt tcaatgcaga aaacccttat
     1141 tactacttac ggcataatgt tcgtgctggt atcactgggt atgcacaagt ctatggaaaa
     1201 tatgtctctg attacaacag taaattacgt tttgatttac tttatatcaa aaaatattca
     1261 ttgatgcttg atttaaagat cttgctgcaa accatcaaga tcctatttga taaaatgtcc
     1321 tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg cagaaattgc ttttccgaat
     1381 gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca atccgaaatt cagcataatc
     1441 gtaccagtat acaatgcaga agcaactatt ggtcgaacga ttgatattct aaaagaattg
     1501 gattatgaag agtacgaagt cgttttagta aatgatggat caacagatca aacacaggaa
     1561 atcattcaag aactgattga aaatgatgaa cgttttgaat tgattactac gaccaatcaa
     1621 gggcccggat tagcaagaaa cgaaggaata agacagtcaa aaggagaata tctcttattc
     1681 ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag attatgatca tttgctagaa
     1741 aagagtccaa aagcggatct tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga
     1801 agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc actatctaac tcacgaagag
     1861 tttataaaag acatgtatca gttaatgaat gaacagttga tgtatgttgt atggaacaaa
     1921 tgctatcgta gcgatattct gaaaaataac caaattctat ttaaaactta taatagttgt
     1981 gaggatcgaa tatttaactt acattattat gaatactgtc atcaagtgat catgaaccca
     2041 aaagtagaat atatttatga atttgaaggt gggaagggga tcactaatca ataccgacct
     2101 aacaagttcg aaacatttaa agaattctat tcattagcaa atactgtaac agacgatatt
     2161 aataaagacg gtatggcggc gcttctttta aaagggacaa cttcagttat tttttctatt
     2221 tatagtacct ctgcaagaac ttcaaaagag aagaaagacg aagcaaagca gatattgagt
     2281 gatccagtga tccgagaagc caagcaaatt gctaagacgg attcttcagc aaaaaaaata
     2341 acaaagttgc tttacaatat gccagctcca ctatttttat ctgctgtaaa agttggaagc
     2401 ttcgttgaag tgaaaatgcc agggttaatg gctgctttga agcgagtata ttagttaatg
     2461 gaggaaaata taatgaaaag aattttgcat tttcagggac gaatgggttt aggtggagca
     2521 gagtctttca tgatgaattt atatcgtaaa attgatcgta caaactatca atttgatttt
     2581 ttgatttatg aagattatgc agatgttcaa gactatcatt cagagattga aagattagga
     2641 ggccgtattt ttgttgtacc taatcctaag aaaaatattt ttaaatattt aatagaagtg
     2701 aataagttac taaaaaaaga atctttttct atagttcaca atcaggttta ttttggcggg
     2761 ggaattaatt tatggttagc caaaaagaat ggaatcagac agagaattgc tcatagccat
     2821 gcaactgaag atggaaaatc tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc
     2881 aaattgttgt tacaaaatgc aacagactac cttgccgtta gtcaagaagc tggtgaaagt
     2941 ctgtttcaaa accatccctt tgagatagtt cataatggta ttgatttaga gctttatagt
     3001 aaaaattcag aagctaaggt taataagaga aaagaattgg atatctcaat gagtactttt
     3061 gttgtgggaa acattggacg attagagaaa caaaaaaatc aatcttattt gttagagatt
     3121 tttttagaaa tactaaaaat gaattctgat tcttacttga ttttagtagg tgaagggtca
     3181 cttagaaata aactgaaagc caaagcagta gaattggata tagagaaaag agtaaaattt
     3241 ttaggagaaa gaaacgatat tcccgagtta ttatctgtga tggatgtttt tgtaatgcct
     3301 tctctttatg aaggtctgcc aatggttgga ttagaagcac aagcagcaaa attaaaatta
     3361 gtcttagcag atactatttc caaagataca aagttaacaa ataatgtgga ttttgtatca
     3421 ttagatgaaa caccacaaga atgggcaaaa aaagtaataa aagagccgta tgctaataaa
     3481 ataacacaag aactaaagag ttatgatgtt tcgtacacta ctgaacaaat ggagagtata
     3541 tatagtagag atcattaaaa atgttcaaga tattttagga gtgattattt ttgaaaatat
     3601 gttatttgat attggcatac aatgatattg gcaacttaaa gagattggtc aatcgattga
     3661 acgaaaatgc agtttttatt attcatatag ataaaaaaac tgatatagaa ccatttattg
     3721 aagcatttaa atcatatgat aatgttgaat ttttatgtaa taaaaaccgt gttaaaattt
     3781 attggggtgg tttttccatt gtacaagcag aaatcaattt agtaaagaga gcattacaaa
     3841 atgagaaata tttaaaatat gtattattat ctggtgctga ttatccaatc aaagataatg
     3901 agtatattta taattatttt aaaaagaata gttcagtgga gtttattcgt ggaatagatc
     3961 tagatcaaat taaacataaa gaattttatt ataaacatat agatgtatat caaaaacatg
     4021 attatcctag aattaatagg aataacacaa ctgcatttaa aatttttaga gctataatta
     4081 atcgttgtct gaggatgatt aaacttcccc caaaaattag acaccataaa tttgatttgt
     4141 atcatggttc tcaatggtgg gctttgtcga aagaatgcct aacagaatta atacaaatgt
     4201 atgaacaaaa tcaacaagac tacctgaatt ttaaaatagg tatgtttgcc ccagatgaaa
     4261 aattttttca tacacttttt tttaattcct cgttcaaaaa taaaaatgtt attggaggac
     4321 cagatttacc acttgaattg aaaaatattg aagaaactac tttacaaact tcgaaattag
     4381 caaatattca tattattgat ccttccatga acaaatggtt tgatgaaagg gatatagatc
     4441 aaataaaaga aagtgaaaaa ttatttgtaa gaaaagttaa atcggattac tctgataaac
     4501 tattaaatag aattgataaa gaaattttga aagttggaaa tatagatgaa tagaatagta
     4561 cctgttttag agaagagttt attaagtcga gatgcgtttc gagtaataaa tttaatcatt
     4621 tgtctgcttt attttatgcc tagtactttt aatgtaacta atatcccgat gaaaatagct
     4681 tttgcttggg gtggaattat agttttgtat gaggttttta taaagaaaaa tttttttaaa
     4741 atgcgttttt cttggctact gattggagca atgattagtt ttatcttaac tataatcatt
     4801 aacagagaaa attttttaat tccatctatt tataattttg gatatttgct gattactttg
     4861 atagtaattt ttcctgcaga cttttcacaa agtgaagagg aaaaaaagaa aaaaatggtg
     4921 tattttaata atgttttcat tatcataatt tttttagctg cgctaatatc tatctatcaa
     4981 tttatttttt tgatttctta tcatgtccca acaggaacgc caggattact tgcaagacaa
     5041 ggttttattg aacatcggtt atttggtgtc tatacaagtc caaatgttgg agctatgttt
     5101 ggatatactt ctattatttt aagtttaatt agtgtattaa tttcaaaaat aacaaaaacg
     5161 attcgtgttt tttatatgat gaattttatc gtacaaatat tttatattac actttcttcg
     5221 tctagaggtg cccaggtagt aatttgttct tttttagtat tatttctttt gttgtttgcc
     5281 aattcaaaat ttagaaagag cattagagaa gtactacctg taaaaatcaa atggatggta
     5341 agcatattat tagttctatt ggtatatttt tcttttggta ctaattatgc taaggatttt
     5401 ttagcaacaa ttcctgtatc gattgataga aaagtagaag ttatagagtc aaatgaatca
     5461 gataaaactc cacttccaga aaaagaagta atattacagc attctagtga taactccgaa
     5521 atttcagctg gtcgttttac gatttggaaa gctgcgctta aagttataag accaactttg
     5581 ttatttggtt atggagagac cgatttctat gggaatagcg atgcaaaatt tttgaaaaaa
     5641 gtagatcttg atactacaga tgtgaatgaa ctaaaaagag cacatggaaa catgcataat
     5701 ggatttcttc aagtacttgt atcaagtggt ataattgctt ttatatgtat ctatgctttc
     5761 tatgtgttaa atatctttag tttactcaaa acatttttta gaagtaaagt gaattatgcg
     5821 tttcttggta ttgttttaat ttttatacta tctattttta tagatgaaat ggtggaggct
     5881 catgtacttt tcaataagcg cgatgtcatt tcaattgttt tctggtatta tttgggaagc
     5941 atttcttact tgtatttaaa aaaaggagaa ttaatatgaa gacagctttc attgcttgga
     6001 ctcctttaca tttgattaat atattaaatg tttatcaaaa tttttaccga gaatcaaaag
     6061 gagatttatt tatttattct gaatttgaaa acgctagaag atatagtgag cgtataaaag
     6121 aattagggct atttaatgat gttgtttttg tgaatcaccg gaaaatcggt tcttccttta
     6181 aacgtaaaat taacttgtta actaaccata atcaattttt ctcggatgat ttatccgttt
     6241 atgatgaaat ttttatacag ggggaaaatt atttcacaaa aatacttttc tccaatgttt
     6301 acaaaaaaaa tccagatgta aaactaaatt atatagaaga tggtttgggt acttatttga
     6361 agaagagtat attttcaaca agtaaaaacg ttcaaagagt ttttaaaata ttaaataaaa
     6421 atagcatctt caaaaaaaaa attgacgatt attttgtcta ttgtccggaa ttgttacttt
     6481 atgatgtaaa aaatataaaa aaaattccca aaatagatga aaacagtaaa ttgatgaaaa
     6541 acatacaatc aatctttggg atagaagaag tttttgtcgg agataaatgt atcttttttg
     6601 atcaaccttt attatctgat ggttatggca tagatgaaac agatattttc gctaaagtaa
     6661 atagtatatt ttctggaaaa aaagaaactt taattaagct tcatcctaga gcaaggacaa
     6721 atcgttatgg tgaaaaaaac atattgaatt ccagtttacc gtttgaagtc ttgctacttg
     6781 aaaatgactt ctcttcaaca cttttgatta gtcctatatc tacaatttct ttttcaccat
     6841 ctatgatgtt tgaaaagacg atcaactcta ttatgttggc taaattaatt ttaaatgaat
     6901 atacagcatt atctgaagat aaaaaagaaa cattgacaaa tattgttctt ttctgtgaac
     6961 attataaccg tctatcagaa gaaaaaattt tactcccaga aaactgggca gaattaagaa
     7021 agttggtaga gtatgggatt atttaacaca ctttccaagt caaaattagt taagtccagt
     7081 ttctggtata ctatagggaa tttttttatt aagggaattt cattcataac tattcctatt
     7141 tttgtaaata ttactactca agcagaatat ggtttgatta ataatttcac ttcatttgta
     7201 tcaatttttt cgattcttgt tgggctaagc ctaaattcgg caattaataa tgcaagtttt
     7261 gactataaag atgacataaa tgaatttatg tcatcggtac tttctctagc tacactttcg
     7321 ttaattgtgt tcttattaat agggaacata tattttttgt tttcaaatgc tttttttgat
     7381 atgaaccaac ttgtatttaa tttgatgtta ttacaaagtt ttgctagttt tgtaattagt
     7441 tatatccagt cttattttac gattaacttt aaccatttta aatatttgat tttatctttt
     7501 atatctactt ttttgaatat tgcaatctct gtagctttga tgcagacatt atattttgat
     7561 gataaatatg ttggcagagt agtcggcgga acactgtcat ttgtgttgat aggtttagta
     7621 atttatttaa gaataatgat aaagggtgct catttttttg ataaaaaaca gtggagatac
     7681 gctttaaaaa tctctttgcc gctaatccca cattccttag caaatatagt tttagcacaa
     7741 ttcgatagaa ttatgattaa ttcttattta ggtcctgctg cggggggaat atattcctac
     7801 attagtaaca taggtattat tctaagcgta atatggatgt ctactaataa cgcctgggtt
     7861 ccatggttct atggtgagat gagtaaaaat aatgaaaaaa aagtgaagaa agttagtaac
     7921 tattatttag tagtctttac tttgattacg atgattgtaa tggtagtttc gattgattta
     7981 gcaaaattta tggctccgaa agactattat tctgggttat ctttagtggt tccgataacc
     8041 ttaggctatt tctttcagtt tttatatagt ttaccagtta atgctgaatt ttatgagaaa
     8101 aaaacatcgt ttattacgat tggtacagta gcaagtgcta tagttaatat actattaaac
     8161 atgattttca tacctaggtt aggaataatt gctgctggat acacaaccgt atttacgtat
     8221 tttttacttt ttatttttca ttataatatc gctaaaaaaa ttacgaataa acaattattt
     8281 gacacaaaaa aaatatgtat cataataatt tttgtatcaa ttttttcttt gatacttcat
     8341 attttaatca atcaaatgtt gattcgttac gttttgacat cattattgat aattagttta
     8401 gttatatatt ttttaaagat taagaatcga attatagatg actataattc ataggaggag
     8461 atataagtgt ttaaaggaaa aacaatatta gtaactggcg gaactggttc cattggaagt
     8521 gaaattgttc gtcagctttt aaaatatgaa ccaaaagcta tcagaattta tagtagaggt
     8581 gaagaaaaac aattttatat gcaacaagag ctctcaaatc acactaattt gagattttta
     8641 attggtgata tcagagactc tcaaaggatt gattatgcat gtcgtgatgt ggatattatt
     8701 tttcatgctg ccgcaatgaa acacgttcca gcaagtgaat ataatcctat ggaagctgta
     8761 aaaacaaatg tgatgggaac tcaaaatgta atagatgcag cgatcaaaaa taatgtaaaa
     8821 cattttgtag gtatcagtac agataaagta gtgagtccta ctaacaccat gggtgcgaca
     8881 aagttattga gtgaaaaatt aataatagct gccaatcagt ataaaggaaa aacagaaaca
     8941 atattttctt gtgtaaggtt tggaaatgtc atggggtcaa gtggatctgt tattcctctt
     9001 tttagtaaac aaataaaaaa caataaagat attactttga cagatgttga tatgacacga
     9061 tttatgatgt caataccgga tgctgcaaga ttagttctgt ctgctgctat gaattcaaaa
     9121 tttggagaaa catatgtctt aaaaatgccg gcaatcaata ttttaacttt agctcaatca
     9181 ttaataaaaa tatataatga tacacatgat gaaaaatata gaggtgaaat aaaagttgta
     9241 ggtatcaggc caggagaaaa attatatgaa gagctaatga ctacagacga aattgaacgc
     9301 tcatatgaaa atgataaaat gtatatcatt ccaccacact atcatgctac tgacgaactg
     9361 tacaaagatt atgaaaaaat taaaatcact gcatactctt cagatgcaac agaattaatg
     9421 agtggacagg aatttattag aatcgcaaaa gagaataatg tcgtgacttg gtaaggagtt
     9481 ttatgatgaa atcagaagta tatgtaattg cagaaattgg tgtaaaccat aatggaagtc
     9541 tagatttagc tttagaaagt attgataaag ctaaagaatg tggagctaac gcggttaaat
     9601 ttcaaacatt taaaacgaat aagctagttt ccaaaaaggc tccaatggca aaatatcaga
     9661 aggataatct taaagaggac agttctcaat ttgaaatgct aaaaaaatta gaattatctc
     9721 aatctgattt tattagaata aaagattatt gtatacaaaa taaaattgat tttttgtcta
     9781 ctccttttga tgaagaaagt gcagactttc taaaacaaat tggtgtacaa ggatttaaaa
     9841 ttggctcagg agatttaaca aatttacctt ttcttagaaa aatagatatg tatgggttgc
     9901 cagttttatt atctacaggt atggctgata ttcatgaggt cagtgaggct gtagattgtt
     9961 tcatagatag tccagttact attttacatt gtacttcaaa ttatccggct agttctgaag
    10021 atattaattt attggcgatt aacacattaa aagaaatgtt cgggataccc gttgggtact
    10081 ccgatcattc attgggatat gatgttggta tttgtgctgt tgcgatgggt gctaaggtaa
    10141 ttgagaaaca ttttaccttg gataaggaac tacctggtcc agatcataaa gcttctttag
    10201 atcctcagga atttgcagat tttgtaaagc atattagaaa cactgaaata tttttaggtg
    10261 atggtgttaa gcgagcaatg ccttctgaac ttagtacaaa agaagtatca agaaaaagcc
    10321 tagtggtcac tgagaattta aatgtgggag atattcttac agagaaatct cttacaataa
    10381 aacgtcctgg aaatggaata gctcctaagt ttttatatca atatattggc aaaaaagtta
    10441 aaagaccaat aagtgcagat agtgttctgc ttgaggaaga tgttgaatga aaaaaatact
    10501 tatagtaagt ggaacacgag ctgattatgg aatatataaa cctgttgctg agaatttact
    10561 acaagataat tgggatgtat catttgttgt ttctggaatg catttatccc ataaatatgg
    10621 atttactaaa gaattgatct tagctgacgg atttagaatt agtggagaag ttcaaagttt
    10681 gtttttagag aacaataagg gaaacatggc tcggtctgtg gcattagaga taaatggttt
    10741 cacaaatata tttgaacaaa ctagccctga ttttgtatta ttactagggg atagaggaga
    10801 aatgttggca gctgcgatag cttgtttgta tcttggaatc aaaacagcac atattcacgg
    10861 aggcgaagtg tctggtactg tagatgaatc aataagacat gccataacga aaatagcatc
    10921 tgttcatttt gctgctacgg aaaaaagcaa agagcgttta ataaaaatgg gggaagattc
    10981 ttggagagtt tttcccgtcg gagctcctag aatagatact attttaaata atagattacc
    11041 tagttttaga gatataaaag aaaaatatca tctaaaattt gaaaagaaaa aatacgtttt
    11101 gcaagtattt catccagttg taaccgaact agaaaatata gaaaaacaaa ttttagcttt
    11161 gatcaattca atggaacaac aatctcaacc aattatttgc attttaccta atagtgatgc
    11221 tggtagcgaa attatcagaa aaatgtacca gaaatattta aaaacggata ttatttatat
    11281 tacgaattta gagccggagg aatacctgac tatattaaaa gagtgcaaat ttatgatcgg
    11341 aaattcttct tctgggataa ttgaagctgc atcattcaaa ataccagtaa taaatttagg
    11401 aaccagacaa aatggacgag aacaatctaa aaatattata aatgcaaatc taaatgaaaa
    11461 tgaaattgaa actgctttga ataaaataca agatcctcag tttataaaaa gcttgaaaaa
    11521 tgttaagaac atctacggtg acggcaacag ttctagaaga ataacaaaaa cattgaatca
    11581 aatattgaat gatggggaag atattaagtg gattcaaaaa caaatagctt attaaaatat
    11641 actagtgatc catacattat tattggggct ggcggacacg caaaagtcat tattgaaacc
    11701 ttaagattag aaaataagtt aatatatgga ttttttgatg ataagcaaaa taaaatgcaa
    11761 gatctgaaat atcttgggaa aataaacgaa ttgaatagct gggcggatag caaaaaaaaa
    11821 atctataaat ttattgtagc tattggtgac tgtgtagtaa gaaaaaagat tataaaaaaa
    11881 ttgaatatgc caagtgagca atatggaata gctgttcatc caagtgctat agtggcaaaa
    11941 gaaatttcaa ttggttatgg aactgttatt tttgcgaatg ctgtaattaa cccagatgct
    12001 gtaattggag agcatgctat aataaacaca ggtagcattg ttgagcatga ttgtagaatt
    12061 aataactatg ttcatttatc tccaggtgta tgcttagcgg gtggagtaca tgtaggtgta
    12121 ggaactcaaa taggaattgg ttctcaatgt atccagatga agaaaattgg atcttggtgt
    12181 atgataggcg cagggagcac tattgttaag gatattccct cacattcctt agcttatggc
    12241 aatccagcta aaattaagaa agaaggaatt aattttgaat tctagaattt tattatcaag
    12301 tcctcatatg ggaggaacag aagaaaaata tgttgctaag gcttttaaag ataattggat
    12361 agcgccatta ggtgaaaatg tggataaatt tgaagaaagt attacaaaat atactggttc
    12421 taagggcgga gcttctgcgt taagttcagg tacagctgcc atacatcttg ccttaagact
    12481 gcttaatgtt ggaaaaaacg atattgtctt tgctccttca tttactttta tagctacagt
    12541 aaatcctatt ttatatcaag gggctgtacc agtgttcatt gactcagaac ctaatacatg
    12601 gaatatgagc cctgttgcac tagagagcgc attagaagaa gctgaaagaa acaaccaatt
    12661 acctaaggca atcatcgtag tgaatttata tggtcaaatg gctgattatg aacgaatttt
    12721 agctattgca aaccaattta atgttccagt gatagaagat gcagcggaat cgttaggttc
    12781 tacttataag ggaatttcgt caggcttatt tgggacaatt gggatctatt catttaacgg
    12841 taataagatc atcacaacct caggtggagg agctctagtg tctagtgata aaaaatatgt
    12901 tgagcattca agatttttag caacacaagc aagagatcaa gcaccgtatt atcaacatag
    12961 cgaaataggt tataattacc gtctaagcaa tatttctgcc ggtatcggac gtggacaaat
    13021 ggaagtattg aatgatagag tagctaaacg aagagaaatt ttcaatcgat ataaggaagc
    13081 tttttctgga attgatagaa ttgaaatgat gccagaagtg gatggcatgg tgccaaatcg
    13141 ttggttgtct actttaacga tcaatgatta taattctatt gatcaagtag ttaacgtaat
    13201 aaataaacta aatgaaaaaa acattgaaac aagagctttg tggaaaccac tacatttgca
    13261 accagtattt caaggggtaa aaatgtatta ccatgataat aatcttagtg tgtcagaaaa
    13321 gttatttaat agcggcttat gcttaccttc tggaagtaat atgacagata gtgaacaaga
    13381 atttgttatt gatgagttta aaaaaatatt caagtaggtg tgatatttta tgaacatagg
    13441 aattattggt ttaggttcca tggggaaaag aagacttaga ctcattcaag aacactttaa
    13501 tgatatttta ctatgtggtg ttgatactaa tgaggcgaga cgaaaggaag ttgaaaagga
    13561 atttggaata ccaacttata atagtctaac agaggcaaaa aatttgtttg aaataaattc
    13621 agtttttgtt tgtacatccc ctataaccca tgaatttatt ataaaagagg ctttaaattt
    13681 agattgtaat atctttacgg aaatcaattt attaaatgaa tattatgatg aaataatcga
    13741 agtagctaaa aagaaagata gattacttta tctatcttct acatttttga agagaaaaga
    13801 aattcaatat attaagtcta aagtgacgca taataaaaaa atttcttatc aataccatgt
    13861 aggacagtat ttacccgact ggcatccttg ggaagattat cgcaattttt ttgtttcaaa
    13921 caaaaaaact aatgggtgta gagaaatatt cgctattgaa ttaccttgga ttatagatac
    13981 atttgggaaa gttaaatcgt ttactgtaat gacccagaaa ttatccaatc ttgatattga
    14041 ttattcggac agtttcactc ttattattga gcatgaaact ggtatagttg gaactttaag
    14101 tgtagatgtt gtttcaagag tagctaaaag aaatttaact ataattggtg aaaagatttt
    14161 aatagaatgg gaaggtaacc cggattctct attttcatgg aatgaagaaa cttctagtat
    14221 gacagatatt caattgtacg atgaggttat gcatgataat aattattcac atactattat
    14281 tgaagatgca tatttagagg agattaaaga atttttcacg cttttggaaa agaaaatgga
    14341 accccaatat acttttgaaa aagataagta tattattaac ttaattaacg acattgaggt
    14401 aaattaatga tgaaagtaat tttttttggg ttagggtcaa ttggaaagag acatctaaaa
    14461 aatctaataa gctatggaaa aaagaattct attaattttg atataacagc ttatagtagc
    14521 agtggaaaaa aagagtttga agaaataacg tatattcaaa atagagacga tttggataca
    14581 cattatgata tagcttttat tacaaatccg acttttaagc atctggaaac actaaatgta
    14641 attgttgaaa aagttgatta catattttta gaaaaacctg ctttttctga gagtattgat
    14701 cttactcaat ataattatga ttacaaaaat atttatgttg ctgctccact gaggtataaa
    14761 aatatcatgg aagttgttcg taatagtatt caaagtaaaa aagtatattc tgtaagagct
    14821 atttgttcta cttatcttcc tgattggaga aaaggtactg attatagaaa taattattct
    14881 gcctttaaat ctatgggggg aggagtagaa ttagactgta tacatgaatt ggattatatt
    14941 ataaacctat ttggtttccc taaatcagta aaaaaacagt ttggtaaata ttcgaatctt
    15001 gaaattaaca gtaatgattt agcaacatat atattgagat acgatgataa aattatagaa
    15061 attcatttag attattttgg aagagttcct acaagaaaat ttcaattgtt tactttagat
    15121 gaatgtatca atgttgactt attaaataat aaaataagtt ttgaagaaaa cattgttaaa
    15181 gaaatgcctg aatctgctaa tgatatgtat gagaaggagt tgaaatattt ctttgaaaac
    15241 gtagtaacag gcaaagaaaa ctggaatgat ttatatcatg caaacgaagt attgaaaata
    15301 gctgaggaga aataaatgga aattttattt actatttgtg gtagagctgg gtctaagggg
    15361 tttaaaaata aaaatttgaa atatttggca ggaaagcccc tagtccattt cgctttagca
    15421 atgatagata tctatcaaca aaaacatcct gaacataatt gcacgacttg tttgaatacg
    15481 gacagtaatg agttagtaga attgacgact aagttaaatc cgtctgtgaa aattgtagaa
    15541 cgtaaacctg aattagctac agatactgta gggaaaattg aagtgatacg tgattcattc
    15601 ttacaactag aaagagaaga taatatattt gatttaatta tagatttaga tttgacttca
    15661 cctttacgtg aagtttctaa tcttgaagaa ttaatagatg aacatctaaa aaatttagat
    15721 aaagacgtct acttttcagt tgtttctgca agacgtaatc cctattttaa tatggtaaaa
    15781 agaattgaaa atgaagttac tatagttaat aaatcaagat acacatctag gcaacagaca
    15841 cctgaagttt ttgaaatgaa tgcctcaatg tattctttta aacctgaatt tctaaaaaaa
    15901 agtaattata tttttgatgg gcaatgtggg ataataaaaa tgagagatta tctaatttta
    15961 gatattgata gtgaagaaga ttttaaatgg ttagaatttt tgtttcctaa atttttagaa
    16021 gattcttcgg agcttcaaga tatctataat tatttatatc ggaggtaaaa ggatgagctg
    16081 ttatcttatc accggtgggg ctggcttcat tggttctacc ttagccaatc atttaggaga
    16141 agaacatgtg gtaatcgtcg tcgatgactt atcaatggga aaagtagaaa atcttgatat
    16201 gacgaaaaat atcactttta ttgagggaga tgtcgctgag aaatccttga tggaagagat
    16261 catgaaagct aataaatttg attatatctt tcatttagca gcagttgcta gtgtggcaga
    16321 ttcagttgcc cgaccagtag aaacacatcg tgtgaatttt gaaagtgtat taatgatttt
    16381 agaattgatc cgcaagtatc aacctgattt aaaacggatc gtgtttagtt cttcagcggc
    16441 tgtttatggg gatgaaccga ctttaccaaa aaaagaagag tccgtgattc gcccattgac
    16501 gccttatgcg gttgataaat tcgctgcaga acaatatgtg ctagattatt gtcatttata
    16561 tgatgtaccc ggtagcgcag ttcgtttttt caatgtctat ggtccaaatc aaaatccgaa
    16621 ttctccatac agtggtgtga tttctattct cgtagatcgc tataagaaac aaatcgtagg
    16681 agaagcaact tcatttacat tatttggtga tggaagccag tctagagact ttgtttatat
    16741 agatgatgta atccaagcat tacttttagt tgcgaatgag gaagctgcac tggggaaaca
    16801 attcaatgtt ggaacgggta aagcaaccac acttttggaa ttgattcatg caatcaatga
    16861 aattctcggt gtagaattga cacttgaata caaagaagag cgttcgggtg atatccatga
    16921 ctctctagca gatattacta agatccagtc aattggctat caaccgaaat atggtatttt
    16981 aagtggaatg actaattatt tgaaaactga aatcaattaa aacatactaa gataagatta
    17041 gagggtaaat tcaggctctt tgtcaataag gactgatgag ttttgtaaaa tcaaatctgg
    17101 gatagaaggg ctctttgtca aatgatgttg gtaaagagaa atcttagaat aatttggaca
    17161 gaacagaaag aagaa
//
LOCUS       DASHRQ010000055        17165 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.058, whole genome shotgun sequence.
ACCESSION   DASHRQ010000055 DASHRQ010000000
VERSION     DASHRQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.058"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(39..405)
                     /gene="ssrA"
                     /locus_tag="U2M88_002055"
     tmRNA           complement(39..405)
                     /gene="ssrA"
                     /locus_tag="U2M88_002055"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(576..1349)
                     /locus_tag="U2M88_002056"
     CDS             complement(576..1349)
                     /locus_tag="U2M88_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM8374416.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1493..1843
                     /locus_tag="U2M88_002057"
     CDS             1493..1843
                     /locus_tag="U2M88_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEM8374417.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1824..2540
                     /locus_tag="U2M88_002058"
     CDS             1824..2540
                     /locus_tag="U2M88_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8374418.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2540..3988
                     /locus_tag="U2M88_002059"
     CDS             2540..3988
                     /locus_tag="U2M88_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEM8374419.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4058..4567)
                     /locus_tag="U2M88_002060"
     CDS             complement(4058..4567)
                     /locus_tag="U2M88_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374420.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4557..5282)
                     /locus_tag="U2M88_002061"
     CDS             complement(4557..5282)
                     /locus_tag="U2M88_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374421.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5540..6880)
                     /locus_tag="U2M88_002062"
     CDS             complement(5540..6880)
                     /locus_tag="U2M88_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEM8374422.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(7047..9167)
                     /locus_tag="U2M88_002063"
     CDS             complement(7047..9167)
                     /locus_tag="U2M88_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEM8374423.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            9397..10575
                     /locus_tag="U2M88_002064"
     CDS             9397..10575
                     /locus_tag="U2M88_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEM8374424.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            10591..11349
                     /gene="aroD"
                     /locus_tag="U2M88_002065"
     CDS             10591..11349
                     /gene="aroD"
                     /locus_tag="U2M88_002065"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEM8374425.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            11372..11857
                     /gene="ybaK"
                     /locus_tag="U2M88_002066"
     CDS             11372..11857
                     /gene="ybaK"
                     /locus_tag="U2M88_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEM8374426.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11928..13181)
                     /locus_tag="U2M88_002067"
     CDS             complement(11928..13181)
                     /locus_tag="U2M88_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEM8374427.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            13298..14647
                     /gene="gdhA"
                     /locus_tag="U2M88_002068"
     CDS             13298..14647
                     /gene="gdhA"
                     /locus_tag="U2M88_002068"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEM8374428.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            14759..16105
                     /locus_tag="U2M88_002069"
     CDS             14759..16105
                     /locus_tag="U2M88_002069"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEM8374429.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(16413..16799)
                     /locus_tag="U2M88_002070"
     CDS             complement(16413..16799)
                     /locus_tag="U2M88_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEM8374430.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(16848..>17165)
                     /locus_tag="U2M88_002071"
     CDS             complement(16848..>17165)
                     /locus_tag="U2M88_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM8374431.1"
                     /translation="NILGDKGYISHKIHEKLALKGITISVPPRKNMDKSEKLDHSLLG
                     KQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYS
                     LGY"
BASE COUNT         5503 a         3098 c         3501 g         5063 t
ORIGIN      
        1 aaggatttaa gcatctagcg aaatctgctg aaatctcaca atggagacgg cgggagtcga
       61 acccgcgtcc aaacacatcg ccacttaaaa atctacgttc atagtcaact catttaagga
      121 ttcactttac agcttgccga gtgacaggca ttctgtatcg ctagtctgat gttctctttt
      181 tcaacttaca gacggaaagt tgaaacgtat cccactaaag ttaggaccct tactcgagca
      241 catgggcgat gccgagagga tctacgctaa ctgtttttag gcagctaaag cgtaagagtt
      301 gttttcgttt ttagcagtta tatttaactg taacgttttt acgtagacgt aacctacgaa
      361 acgcaattca agctcgactg tgcctgtcga atccgtaacg tccccgttag ctaacaaggg
      421 ttacgtgtat ttttcataca aaattagtat agcatatatt gggataaatt caataaaaaa
      481 gcgaatcata tcccattatt tatataataa ctgccccttt cttaagaaaa aataagcgat
      541 tttcttaaga aaagaggtag cttcaaaaaa agatcttaaa tcagatcaaa atacttcaat
      601 gcgtggacaa ttccgccttc tgtatttttc tttgtgataa aggtagaaag ttcttttaat
      661 ccgtcatagg cgttgcccat agcgattttg ttgtcgcaag cttccaacaa ggcaaaatca
      721 ttcggaccat cgccaaatcc aaaagtagga actttttcca gctgaagttc tttttgcaga
      781 cgtttgacac ctgccccttt tgagattcct ttcttcactg tatcgatcga gtaaggacca
      841 ttccgataaa aagttagctc aggaaaatgt tcgtgatagt attcgtcacc tgattgagat
      901 aagatcagca acatatttac tgtgttttct ttaaagccta aaggatcaat ttccggaaca
      961 tctgaatgaa tgaagtcgta agcattgatc attgtttggc tatgaccggt acaccagatt
     1021 ttctgatcgt tatagtaaga aagttcatga ccgttcaact gtacatgatc atacattttt
     1081 tgacattctt ctgaagaaaa tgtttgagaa tagatttctt cgccgtctac tcgaatgaaa
     1141 gaaccattca tggtgaccgc agaagtaatg cccgcatctt ccatgataga gtggatttct
     1201 gcttctgtac gtcctgtagc gatgacgggg agaacattgt tttcttttaa tgcgttcata
     1261 gcttttgcca cttcgggtgt gatttttgat ttcgcatcta gtaatgttcc atcaagatca
     1321 aagaaagtga ttgcacgata atttgacaca aataaaactc cttttttatt ggaataagtt
     1381 tatcttaaca aagaatgaac aaaagacaaa cctttcgtga agaattcctg aacatattgg
     1441 ttcagtcaac aatttagagt atactataag aagagaattt aggagggaaa gaatgatttt
     1501 tttcagtatt ggtatcttat tcttaataat tggcagtctt tttcggattc tgccctcaaa
     1561 aggaaatctg cctttttatg ggtatcattc gcctcttgcc gcaaaaacag acgctcattg
     1621 gcggctggct caaaagacaa gcggaaactg gttttttctt atgggcttac tgatggcatt
     1681 gatcggctat tatttaaaga catctgggca tacgaattat tttctgattg aaatgcttct
     1741 gctagtattt ccaattatgc ctatttttat tatgacagaa aagaaattac agaaatatga
     1801 cctggaaact ggagggaacg ataatgaata ttttaatgat tgaagataat caatctgttt
     1861 ctgaaatgat gcagatgttt ttcttaaatg aaggttggga agcaactttc aagtatgatg
     1921 gaaaagaagg gctcgatgcc tttttagaaa acccacaaca ttgggatatg attacactag
     1981 acctgaattt gcccacactg gacggtatgc tagtaggaag agagatccgc aaagtttcaa
     2041 gtactgtgcc gattatcatg ctgacagcta gagattctga aagcgatcag gtgatcggct
     2101 tagagatggg ggcagatgat tatgtcacaa aacctttcag tcctctgacg cttattgctc
     2161 gaatgaaagc tttgcatcgc cgtagtgagt tggcagaagc aagagaagaa gcagtagttt
     2221 cagatgatca ttttgatatc gagactagtc atttcaagat gaatacgaaa acacgagaag
     2281 cgtatctgga caaccggcca attgaaggac tgacgcctaa agagttcgat ctattgttta
     2341 ctttagcaaa aaaaccacgt caagtattct ctcgggagca gttgcttgaa ttagtctggg
     2401 attatcagta ctttggtgat gaacggacag tggacgccca tatcaagaaa ttacgacaaa
     2461 aaatcgaaaa ggtcggacca caagtcatcc aaactgtttg gggagtcggc tacaaattcg
     2521 atgattctgg tgttgcctaa tgcgttattt gtatcagcaa ctcttagctt tctgcggaat
     2581 ggtcgcgttg atcttattga tcgttggagt ttcttttaca caactgacaa aacaaacgat
     2641 tgaagaaaac aattaccagc aactgtttgg atatgctgaa tcagttgaaa agacaacgca
     2701 aacctatgcc gatacgctcc cgcagtttag tcaagatcag gcgtttcaaa atgcattgct
     2761 gctgacagag caagtactga cagaacaaaa tgtgaacttt atatttatcg ataaaaacga
     2821 acgtgtcatt tatccgacta atacagcttt actgaatttt accttaacag ctgatcaatg
     2881 gaaaaatctg aggaatggac gtcaagaaaa attcacatcg aataaaaata ttttaggaca
     2941 agcacaggcg acctcttacg cacttgtgcc atttaatttg aatcatgaat tctatggagc
     3001 acttgtagtt agtcaacctg cgcgaaatat cgataacagc gttcgtccaa ttacactgaa
     3061 cctgttcaaa ggatttattt tttccagtat cgttgcagtt attgccagct atgcttttgc
     3121 agcattccaa gttaagcgta tcaatcggtt aaggaatgcg acgaaagaag tgacgaatgg
     3181 taattttgat gtacagctcc ccgtacatga caaagatgaa tttgatgagt tagcagatga
     3241 tttcaataaa atgacgaatt cattgaaaga atctcaagca gaaattgaag aacaagaaaa
     3301 tcgtcgaaga caatttatgg cagatgcttc tcacgagatg agaacacctc ttacgacaat
     3361 caacggactt ctggaagggt tagaatacaa tgcaatccct gaaaatcagc gagaaaacgc
     3421 gattaaattg atgaaaaatg aaacagagcg tctgatccgt ttggtgaatg aaaacttgga
     3481 ttatgaaaaa atccgtacga atcaaatcag tatggtcatt aaaaaattca atggaacaga
     3541 aacattgaaa aatattgttg cacagttaga agctaaagca gaagcagcag gagatacatt
     3601 gacattgaaa gccgttgatg atattgatgt ttacgctgat tatgaccgat ttgtccaaat
     3661 catggtcaat attattcaaa acgcgataca gtttactgaa aacggccaaa tcacgattac
     3721 tttagaaaaa ggctatcttg aaacgatcat cacgattgaa gatacgggaa ttggaatgtc
     3781 tgaacaacaa atgaaaagta tctgggatcg ttattataaa gtagatcctt caagaaaaaa
     3841 tacaaaatat ggtgaatcag gattaggttt accaatcgtg cagcaacttg tacgcttgca
     3901 taaaggaaaa ttagaagtag aaagcgaact aggaaaagga acaaaattcg ctgttcgttt
     3961 gcctgacgta gaaatcaaaa aagagtaaaa aacaagcggt ataaaagatc atgaatcgat
     4021 cttctatacc gcttgttttt actataaaat gattgattta gcccgcgggc tctaaacgat
     4081 caatataagg actttgagca atagtagcat catgagtaaa gagtaagata ccttgatttt
     4141 cttcatgagc taaataagaa agcaagtgga gtagctcttg gatctccaat acactaagtt
     4201 catcaaatgt atcttcaata acaatattgt ttcgcttgga aagcaaggca tgaagtaatt
     4261 gcagctttac tttttcaaaa gacgtcaact cagctgccgc acaattaagc aaggaaggag
     4321 agatatcaag tttttcttga taggccaaac gatcttgttt gatttctttt tccggtacat
     4381 ccaacaggac atggtcttta agattcagat aaggaaacat cggccaattt ttttggatga
     4441 tcgcaaaatt atccagtttt gcaacaagaa cctctttttt caagtgctcc aatattttat
     4501 gctgtttttc gagaggacac aaaataacgg tcaagtgttc agcatgaatc aaatattcag
     4561 gtttcattac gtccaggcct cctactttgc aaatgatgga taaaaaaagt gaaagggaat
     4621 agactaagaa tgagacagca tccactgagc ccaataagag tttgggcaaa tcccttaagt
     4681 atatctgtga aaaaaagatc attttggaaa tcgatggtaa aaaaagtctg ttgatccatc
     4741 ggaattgtaa gtccgcttac cgaagtcaag atatcacgga tatctggcag ttcagtagtc
     4801 tttaagacaa gaaaattgat cctttgaaga agcgcttcat aaactgactg gaagcttacg
     4861 atccacaata aaaccaagat acaacaaaga aagagaggaa agaacagctg ccagaaaata
     4921 aaaaggaata atttggtttt agaaagacca gtcaggtgcc aattgatcaa ttcctgcttg
     4981 ttttgcctga ctgaatagta aaagaaaaag ataaaaagga aggcaaatag aaaaaataaa
     5041 ctgaatatgc cttttatata aaatcggcta gctaattcta aagccggaag aagctctgta
     5101 gatgcagaag cgccaataaa tgaaagccga tcttggactt gattggctaa agccgattgc
     5161 aaatggtaaa gagtcaggca tgctatcaat aaaaaagcag aaaagcttga aaataaacaa
     5221 tagagtattg tctgcttttt gtaataaatt aatcgagcag tagcatagcg gattattttc
     5281 atacttttca cctctataaa aagtatagaa aatgaatatg aataaagtat gaaattcctt
     5341 tagtgaaatc acgaattaaa accagaaaat attttgtaaa aagcaagagg ttgtgaagaa
     5401 gctgccctat tctattaccg tagatgcaag cttttgaaca ctgtttattc gaaaagagga
     5461 gcagcgacaa taattttggg ctcttcgtca atgagtgttg gtgaagaaat tcaatagaat
     5521 aatcgagata ctcacacggc tagacagcta tcttgtctag ccgtgttgtt gttctggctt
     5581 tagtcagttg ggtagtgttc ttagttttct tgaaatattt tcctcgatat aaaaagattc
     5641 gttctcggta gttttgaaag tttctaaagc cataggcaat tcgttttagt actttgatat
     5701 ggttgttcaa acattctata gggccgttgg aatagggaac tcgcaacgct cgtttgattt
     5761 ctgttcgata tttcttgaag gtcgtgaaca ctggttgata gtgctctggc aactccttgt
     5821 agtcttcttt taggagcgcg acgaattcag gaacatcttg tcttctgata gcggaaagaa
     5881 aaacctggta gacttcgtag cctcgcttta aggagtcatc ataatataat agccgatcga
     5941 ctatctccgc ttctgtcaga tgatctcgaa atgacggacg ccagtagcga tgctgaccat
     6001 tcaattccca cgcattcttt tgaagtagtc tccagtattt ctttaagtgc ttcgaccgac
     6061 gatccccttt tttaaaggtc ttcattactt gaattcggtg gttggtaaag gcccgaccga
     6121 tgtgttggct aatatgaaag cgatcggcga cgattcgggc attcggaaaa cactctttca
     6181 ccaatgtgcg atagggtgta tagaaatctg aaaccactag acgtacttgt tcacggactt
     6241 ttctagggta gcgcaagaaa tagtcgcgta aaggattgag ttgccgattt tccacaatat
     6301 caatcagttg atgggtcttg ccgtccatca tgacaaagct catggctccc gagacgtttt
     6361 tcaccgattt gaattcatcg aaacacacca cttctggtaa cggctggttc aaagaaacgg
     6421 ttttaggccg aaggctgcga agtattcgaa tgacagtggt cggagaaatg tgcttcatac
     6481 gtgcaatcaa agacatagaa ataggctcac ttaatagctc aagaatggct tgtttgactc
     6541 tgcgggcaat ggagcagcga cgatcgctaa ctgaatcttc tgctaaaaaa gtctttccac
     6601 agtgtttaca tttgaagcgc tgtttgttaa tccgcacaat ggtcttgtag ttactgacat
     6661 cattcaataa gatggatact tttttccagc cccatttaat gatcgtttgc ttattttgat
     6721 agtggcaatg gaagcattcc tctggttgat aggtcaatgt gccatcaaaa acaaaacatg
     6781 tttcgtcatt aatgcgctct ttgcgaaagg catcttcatg aaaggtaaga tttaggtcta
     6841 gaatatctag taactctttg gtaaactctg aataggacat cttgaaacac tcctttgaat
     6901 ggttttcgtc gactttattc taaaggattt caaggtgtct gtttgcgttt attagaaaaa
     6961 agaaaaagta ttggcaacag aaggcgtcac caacactaga tattatagat cctaattttg
     7021 tcgctgctcc ttttaaattt attttatcat tcggaagaac ttgtggtcgt tccgtgaagt
     7081 tcttggtacg tttgcgtttt ataaagatca acagttgatt gtccgttgtg ttcactaaag
     7141 acactagttg acttgtcacc aagagatttt tcagcttctt ttaatttctc taagccattc
     7201 ttgtagtcat aatcactggt atcaattggg tctaatccac tattcgtata gaaccgaaga
     7261 agatcaccat tattgatttg gtcagaaaca gctaactgtt tatcaacttt ttctttcagt
     7321 gaatcaattt cattctgtac ttcctcagta ggttcagtaa tagcttcacc agtttcgtta
     7381 ctatagatcg taccaccgta gtaagtatat tttggtgtaa caaaatcacc atcacggaaa
     7441 gaaacaagct gttcatgatc tttcgagaat agatcttgtc ctaactgaat ataatttttt
     7501 gtatctacac ctagtaagtg aagtaatgta ggcaaggcat caacttgtcc gccgtaagtg
     7561 tgattgattc cgccctgtgt ttgtccaggg atatggatca tataagggac acgctgcatt
     7621 tgtgcattgt cgtagtttgt ccaagtagaa cttgtttttc caactaattc agctagattt
     7681 ttatttcttg aattcgagac accgtaatgg tcaccgtaaa ggacgatgac tgaattatca
     7741 tacaagccgc tttctttcaa ataattgaag aattcttgta cggcggtatc taaataattg
     7801 gctgtcgcaa agtaaccgtt gatcgtttca tcatttgtat tggctaacgg gaatcctgct
     7861 tcgtcattgg tgaattttga ataaggataa tggttagata cagcaatgaa ttttgaataa
     7921 aatggttgtt gcagacgttc caagtattgt acggattgtt ggaaaaatgg tttatcatgc
     7981 aacccgtatt ggaacgaatt gtcatcatta acatcataat aagaagaatc aaagaaataa
     8041 tcatatccta aattcttgta tgtctcgttt cgattccaga atgtaccagc gttcccatgg
     8101 aataccgcac taatgtaacc ttgtgtttga cttaaaatat caggtgcagc ttggaaagta
     8161 ttttttccgc ctaattgtgt gaacaaggag ccttgattca agccaaacaa tgaattttcc
     8221 atcagcgttt ccgcatcgct tgtttttcct gcttttactt gatggaaaaa gttgtcaaaa
     8281 ctgaacgtgc tgttgctgtg gtaaagacta ttgatgaaag gtgtaacttc atgctggaga
     8341 ccattctcat ctgtcaattt ataatcgatc aggaattgct ggaagctttc taaatggata
     8401 taaatcacat tttttcctct agcgagcccg aacaaatcgt catttggtgc tgcataatgt
     8461 tcttttacat aattttctac ttctttcata tcattgggac ttgcttctgc ccgcacttgg
     8521 tttgtctggt aagtttgaac accgtcataa accatgaaag cattcaagcc aagatatttg
     8581 accaaataat cacgagagaa tgttcgagtc agcaattctg gacgatcagc ttccgccata
     8641 aaaagatttc ctgagaaaat cataactgct aatgtagaga tcgcaaaagc cattcttgct
     8701 cggatcgggc gagggtccaa cttgattttt tttgcaatca gcagaacagt caagataata
     8761 aaatcgatcc aatagatcac atcatatggg cgaaacagtc gaatggcact ttctcccaga
     8821 ccactcgcta ctttcccagc acctaaaatc gtgttgatcg taatgaagtc cgtgaattct
     8881 cgataataag tgacattcga aaaaacaagc aatgtcaaaa gaaaataaat gaccatcatc
     8941 gtgatataag caactctagg gcgtctgata taaagagcaa ttccaagtaa taacaatgta
     9001 gttgcaatag ggttgataaa caagataaag tattgaatgc cactttcgat tccgagatgg
     9061 aaatcaacta aataggcaaa aatcgttttc aaccaaagta aaacgactag taaagaaaaa
     9121 aatccgagcc tcttattcaa aaaggcaggt gtttttatac atttcaagat aatcttcctt
     9181 tctgcacttc ttaatcacag aagtgtcctg aaaccatgtt acacagctca cttattctca
     9241 tttattttac tagtatgaca atatagtgtc aattgaaacc agaagtttat gctgtttcat
     9301 taaggttttc tttaagcttc atgaaaaaat gagcagtaag acaagtccta tgttttttgt
     9361 tatacttggg aagagaaagc taaagcaggt gaaggaatga aagtaaaagt aaaaaaaaga
     9421 gcaagcaatc gattgaaaaa aggatatcca ctgatccaaa aagaagacct tgctcatccc
     9481 caaaaagaaa ccacgcaatg ggtggaatta gttgattcgc aaaaccaatt tatcgcaaaa
     9541 ggatatctag gaaagcagaa taagggaatc ggttgggtat tgagccagca gaacatccca
     9601 tttgatcaat ctttttttga agaatttttt gtgaagtcaa aagaaaaaag aactttcttt
     9661 ttttcagatg attctactac cgcttttcgt atattcaatg gagaaggaga cgggatcggc
     9721 ggtctgattg ttgattatta tgcaggttat gctgtttttt cttggtataa cgaaacgtta
     9781 tatgcacatc gtgaattatt gattcaagct tttcgtgaaa tctacccgga aatcatagga
     9841 ggctatgaga aaattcgatt tgaatcagta aatctgcctg agagccagtt catttacgga
     9901 aaagaggcac cggaaccctt gatcgtaaaa gaaaacggag taaaatttgc cgtttattta
     9961 aatgaaggat taatgactgg gatcttttta gaccaaaaag aagtacgggg atcattagca
    10021 gaaggtttcg cttctgggaa aacagttttg aacatgttta gttataccgg cgccttttct
    10081 gttgctgccg caatgggagg ggcaatcgaa acaacaagtg tcgatctagc aaaacgcagt
    10141 ttaaaaaaaa cacaggaaca gtttgaagtg aatggtttgg atgtcgatca gcaaaaaatc
    10201 attgttatgg acgtattcga gtatttcaaa tacgccaaaa gaaaacaatt tttatatgac
    10261 atgattgtat tggatccacc aagttttgcc agaaataaga aaaaagtctt tcgcgtagca
    10321 aaagattatg gggaactggt caaggatagt ctggatattt tagcagaaga cgggatactt
    10381 attgcgtcta caaatgctgc gaatgtctcg atagaacaat tccaaacaat gattgaagag
    10441 gaattccatg ccaaaaatgt ttcttttgaa caacttgctt tatatcgtct gccaaaagat
    10501 ttccagacga cggatacctt tttagaaggc aattatctga aagtgtttgt ttatcaaatt
    10561 aaaaataacc attagaagga gcgatgcaga atggttcggg taaagggatt gaatatagga
    10621 agcggtagac caaagatttg tgcgcctctt atcggggccg tccgagagga agtattacgt
    10681 gaagcagttc ttgcacgtga agcaggggcc gatttagtcg aatggcgagt cgatcattat
    10741 cgtgaagttt tttcttttga tgaagtagca gaaacattgg ttcttatcca tgagatgctt
    10801 gatggactgc cgttattatt tacgtttcgt accttagctg aaggcggaga acaagacata
    10861 tcgatccgtc agtatcgcga cctttatgaa catgtgatac acacaggatt agtagatatg
    10921 gtcgatatcg agttgttcaa agtagaaagt ctaggaaaaa gactgataga ggaaatcaag
    10981 tctttaaaca ttcctttaat tatcagtagt catgatttta aagaaacacc agcagaccct
    11041 gtcttgcttt accgattgaa tgttatggaa cattttggag ccgatatcgg aaaactggca
    11101 gtaactccca ataatgaacg agatgttttg cggttgatgg agttgactag acgggcaaat
    11161 gcttttttct ccatgcctat tattacgatg tccatgggaa atcttggtaa aatttctcgt
    11221 ctggttggcg gaacgacggg ttccgttatg acatttggtt ccttaagcag cgattctact
    11281 tctgctccag gacaattacc ggtcaaagca ttgaagcaaa ttattcaatt attaaatgaa
    11341 ccgttgtaga acaagtttga ggtgatattt tttggcaaag aaaaaaatag taaaaacgaa
    11401 tgcaatccgt atggtcgaac aaaaaaagat tccctataca gagcacgaat atgagtggga
    11461 cgaaagccac ctaagtgcgt catcggtcgc tgaacagctt ccagaaagcc aatctcgtat
    11521 atttaaaaca ctggttgctg tgggcaatgt gacggggcct cttgttgctg tgataccggg
    11581 agaagccgaa ttgaatctaa aaaaattagc aaaagtttca gggaataaaa aagtagaaat
    11641 gctccattta aaagatttgg aagcaacgac cggatacata cgtggtggct gctcgcctat
    11701 aggtatgaaa aaactatttc ctacttattt agatcaaatt gcagaaagct atgaacaaat
    11761 cattgtctcg gctggcagaa gagggctaca gatggagtta gcacctcaag atatctgtgc
    11821 attaacgagc ggacaatttg cagatataaa acaataaatc atacgtataa aagaaagaag
    11881 gaggccggga tgttgtttgt ccagtctcct tctttctttt ttatacatta tgctctttgc
    11941 ccggcattca ttcctgctac atgccctgta gcgaaagccg ctgtgatatt aaagccgcca
    12001 gtataaccat tgacatccag taattctccc gcaaaataca gaccatcaat gattttactc
    12061 tccaatgttt taggataaat ttcctttaga gaaacccctc cgccagttac aaatgatttc
    12121 tcaataggaa aagttttgtt gattgatagc ttaaactctt tgcatagttg aacaaaatct
    12181 tgaatttgtt tttgagaggc ttgttgtcct gtcagatgat ccatttcaag ccgctcaaga
    12241 aagaaaacta acaatcgctc aggcaatata ccatgccaag cattgaccaa gttctttttg
    12301 gttgctttac ttttttctgt caatatatga ataagctctt gtttggtttg tgtagggaaa
    12361 caatccagag aaagtgtcac tggttctccc gttttctcta attctttatt gacaaaactt
    12421 gaacaacgca gtgcagccgg accagaaata ccgaaatggg tgaaaagtaa atccatcgta
    12481 tgttcagtca atacacgacc ttttttattt aaaacgcgta aagtgatgtc ttgtaaagaa
    12541 agcccttgaa gtgttttctc ctgaatatac ggttcatccg atatcaaggg ggactctgtc
    12601 gcataaagag gcgtcaccgt gtgacccact cgcttcacga tcttgtaccc atctccagta
    12661 gaaccagtag atggataagt ccgtccacca gttgttaaga taatggcagg agcatggaac
    12721 tcttcgaatt ccgtgcgtat accgtaaatt ttatgttctt tgtggatcaa tttctctact
    12781 cgagtagaga atagaagcgt cacatctagt tctttcagac gattgagcaa tgcttcgata
    12841 atcgtttttg atttatttgt tacaggaaac atccgtccat gatcctcttc ttttaaatgt
    12901 acgccttgtg attcaaagaa gttcatgata tcgaaattat tccattgcgc aaacgtacta
    12961 tataaaaact ttccatttcc aggaatatgt gcaatcaaat catcaacagg acgattgttc
    13021 gtaacattgc accgaccacc acctgtcatc agtagttttt ttcctgcttt tttgtttttt
    13081 tcgatcaaga gtacgcgtgc gccttgttca gctgcactga tcgctgccat catcccgctt
    13141 gttccagcgc cgactacgat cacatcatac agtttttcca ttccctcacc tcaaggaaag
    13201 tgtagcatat ctgaagaaag aaaacagaaa aaaatgtttt gagccaaaag agaaaatgag
    13261 ctaaaatgag ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt
    13321 tcaagcaatt caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc
    13381 aatcgatgaa ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga
    13441 aaagaatatc ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg
    13501 gcaagatgat gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc
    13561 tatcggccct tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa
    13621 atttttggct tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg
    13681 aaaaggcgga agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg
    13741 tcaaagtttc atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg
    13801 agatatcggt gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa
    13861 aaaatatgat actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg
    13921 tacggaagct actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa
    13981 agattcattt aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc
    14041 aatcgaaaaa gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta
    14101 catctatgat ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag
    14161 acaacgtttg acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc
    14221 tgtttggaca ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat
    14281 agacgaaagc ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc
    14341 taatatgcca tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg
    14401 tccaggaaaa gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa
    14461 cgtccagcga ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga
    14521 aacgatttac catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct
    14581 tcttggagca aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt
    14641 agtttgaaaa atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa
    14701 ataataaaaa aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat
    14761 ggcacacatt cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg
    14821 gtacatgcaa agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg
    14881 aagcgacttc cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg
    14941 tatcaaagaa gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat
    15001 cggtggttct tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa
    15061 tttattagat agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag
    15121 ctcaacatat attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt
    15181 tatttcaaaa tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct
    15241 tcttgttaag aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa
    15301 agcaaaaggt gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc
    15361 tgatgacatc ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt
    15421 tagtggtgca gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc
    15481 aagcgacaaa ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta
    15541 ccgtaaaggc aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc
    15601 agaatggtgg aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc
    15661 ttcaagcgca aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg
    15721 tcgcaatatt tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc
    15781 tgaacaagca gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt
    15841 aaatacaaaa gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt
    15901 gctagtcaaa attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga
    15961 aattgctgta ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt
    16021 agaagcttac aagaaaaaca tgttcgcact tctaggcaaa ccaggctttg aagatcttgc
    16081 taaagaattg aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg
    16141 atagaaacat gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg
    16201 catcgtttat tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat
    16261 agaaatcgat tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct
    16321 gacttcgaag gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca
    16381 cttcgagtgc ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca
    16441 gatgagggta ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct
    16501 ttcaactgca agatcgttcc ttcggggtaa gcagaaccat tggcagagtc accagaagta
    16561 atgacatcga aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat
    16621 ttaaaacctc ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt
    16681 ggagctttga ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa
    16741 cctttccatc ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg
    16801 aaagatctcc ttttttattt ttattatacc cgtggtgcta gttaatttta atatcctaaa
    16861 gaatatctca aagttccttc aaaacgtcgt tgtgctcgac ttaatagaac actgtaagcc
    16921 agtaatatgc tttcaaatct actctctagc cctttaacag aacgtgagtt gaaattttga
    16981 caacctaatt tttctaagga agaaaaaaca gtctcaactg tttttctttg ttttccaagt
    17041 aagctgtggt ctagtttttc tgatttatcc atgtttttac gtggcggaac ggatatagta
    17101 atgcccttaa gggcaagctt ttcatggatt ttatggctaa tatatccttt gtcccctagg
    17161 atatt
//
LOCUS       DASHRQ010000056        17005 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.059, whole genome shotgun sequence.
ACCESSION   DASHRQ010000056 DASHRQ010000000
VERSION     DASHRQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.059"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..124)
                     /locus_tag="U2M88_002072"
     CDS             complement(<1..124)
                     /locus_tag="U2M88_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HEM8374432.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
     gene            complement(228..539)
                     /locus_tag="U2M88_002073"
     CDS             complement(228..539)
                     /locus_tag="U2M88_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEM8374433.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(541..738)
                     /locus_tag="U2M88_002074"
     CDS             complement(541..738)
                     /locus_tag="U2M88_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEM8374434.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1132..2859)
                     /locus_tag="U2M88_002075"
     CDS             complement(1132..2859)
                     /locus_tag="U2M88_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEM8374435.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2852..4045)
                     /locus_tag="U2M88_002076"
     CDS             complement(2852..4045)
                     /locus_tag="U2M88_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEM8374436.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4232..4876)
                     /locus_tag="U2M88_002077"
     CDS             complement(4232..4876)
                     /locus_tag="U2M88_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM8374437.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4970..5104)
                     /locus_tag="U2M88_002078"
     CDS             complement(4970..5104)
                     /locus_tag="U2M88_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEM8374438.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="U2M88_002079"
     CDS             complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="U2M88_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEM8374439.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7235..7708)
                     /locus_tag="U2M88_002080"
     CDS             complement(7235..7708)
                     /locus_tag="U2M88_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEM8374440.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8109..8333
                     /gene="nrdH"
                     /locus_tag="U2M88_002081"
     CDS             8109..8333
                     /gene="nrdH"
                     /locus_tag="U2M88_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEM8374441.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8492..10651
                     /gene="nrdE"
                     /locus_tag="U2M88_002082"
     CDS             8492..10651
                     /gene="nrdE"
                     /locus_tag="U2M88_002082"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEM8374442.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10701..11666
                     /gene="nrdF"
                     /locus_tag="U2M88_002083"
     CDS             10701..11666
                     /gene="nrdF"
                     /locus_tag="U2M88_002083"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEM8374443.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11755..12894)
                     /locus_tag="U2M88_002084"
     CDS             complement(11755..12894)
                     /locus_tag="U2M88_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEM8374444.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13203..13916)
                     /locus_tag="U2M88_002085"
     CDS             complement(13203..13916)
                     /locus_tag="U2M88_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEM8374445.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="U2M88_002086"
     CDS             complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="U2M88_002086"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEM8374446.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15105..16637
                     /locus_tag="U2M88_002087"
     CDS             15105..16637
                     /locus_tag="U2M88_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEM8374447.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16828..>17005)
                     /locus_tag="U2M88_002088"
     CDS             complement(16828..>17005)
                     /locus_tag="U2M88_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM8374448.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2909 g         5323 t
ORIGIN      
        1 aaaaaacaca ttccactaaa tttgatgccg gttttccttg aggttttgtt ccagcacctc
       61 ctttaatttt tttaagctct tgatctgaac attgtttagc atttagtcta tttttttctt
      121 ccattacgat tccccctaca aactataaat acttttatgt cactgattca atatacctat
      181 ttttctaatc aaaaaatttt gagacatatt taatattata agtatcatta aaattgagat
      241 ttatctccat aatctgctcg atataaacca tttattccag cagttcttcc aatttcactc
      301 aaatttctta ttaattcctc ttgatattca gtgaatctta ttttattagt ataagcagta
      361 aaatatagat aattcaccaa tctatttatt aaaggactgg gatctaattc atatttttgt
      421 ttttctaatt ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct
      481 ttacttatag atatatcatt atataattca tgaacaattt gcttagcatt ttttttcatt
      541 ttagcacttc cctggaattg ctccacctaa aaaaccacct atagacattc ctgcaataca
      601 agtagttgcc ttggcccaat cgaccgtaca tttattttta gtgcaataca ctccatttcc
      661 ataatatttt ccactatgag tggtaccccc atagataagt tgtgtctctt taatagacaa
      721 aatttttaaa tgtttcataa tctaatcacc cctaaattaa tgtttttaga ctattattct
      781 aacattcatt aaatttcaaa aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa
      841 taagaattta atcactttta agtatataaa ctatgtatat tctttaatat tttttgagaa
      901 tgcacttcgt acataataaa ctttaatcac attagtcttt catacctacg atcgatctct
      961 ttgactcgca cccataaatt cataatagtt tgtcggcgtc tttgaacgtt tcgaagatga
     1021 gctacgcatt ctcttttttt atagacaaaa aagatagaga cgaacatgga gcttccaaat
     1081 ttggatattt gatccaattt tggaagctcc atttatttcg tgtctgtctt tttatttttt
     1141 ccagctttcg actaattctt ggttctcttt gatccagtct tttgctgctt gctgtggatc
     1201 ttttccattg ttgatttcca acataacttt ttccatatct ttttctgacc aatggaaatt
     1261 gtctaatact ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg
     1321 gatctgctct tcgcttccca ttccgttttc aggatcttct aagtatttca gatcatattt
     1381 ggcaaacatc caatgaggtg tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc
     1441 ttgacctagt gcaacggtca ttgctcctga agaagatgtc tctactttcc agtctttcaa
     1501 gttgtcatat ttttggatcg tgttttcagc agccgtaact acaccagctc ctggctcgat
     1561 tcctatgatt ttcttacctg cctgatcggt taactcatcg atcgaattta catccatgta
     1621 ggctggcact gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt cttcgacttg
     1681 atctttatat tgtgcatatt gtgaaccatg agttttaggt agccaagctg aaaccatggc
     1741 atctgactcg ccatttgaaa cagatttcca catgattgaa ttatctaatg gcgtcatcgt
     1801 tacatcatag cccatttgtt tcaatacttc accgactaca tttgttgaag caacttctgt
     1861 atcccattca acataagata agttgatacg ttttgtttct ttggcagatg aaaaactccc
     1921 tataccaatc gcacctaaga tcactactac tacagcacca atgatcagtc cttgacgttt
     1981 tttacttttc tgtgttgttg ctcctgcagc ttttttattg tttggctggt tcaaatgctg
     2041 tgtaaaacgg tcaatgatga ttgctaagat aactaatgca accccgttga caaatccatt
     2101 cccgacttgt gctctttgca atgctgataa tacgccgcgt cctaagccgg gtgcaccgat
     2161 catagaggca ataacgacca ttgaaagtga cagcattgtt gtttgattga tccctgccat
     2221 gatcgtactt ttagctaaag gcaattctag tttgaatagt ttttgacgac ttgtgctccc
     2281 gaatgaatca gaagcttcta ctaattcttt aggaacttgt cgaatcccta aattcgtaaa
     2341 acgaacggtt ggtggcagag caaagatgac tgaggcaaat acccctggaa ccatcccgat
     2401 accgaaaaat gcaactgctg gaatcaaata aacgaatcct ggcattgtct gcataaagtc
     2461 aaggatcggt ttgataatac tttgggcttt actgcttttt gccatcaaga tccctaatgg
     2521 taccccaatg atgatcgaga tcacactaga taaaagaaca agtgtgacag tattcatcaa
     2581 atcattccat agaccttggt tataaataaa ccatagaccg attaatgtga acaacgtaag
     2641 tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc aataacaaga taaacaaagg
     2701 agctggaatc actaacagta aatttgtaat ccccgacatc actgcctgtc caatcgtttg
     2761 taaaaatgaa aataggccag aaaaagttgt cgtaaaccat tctgtgatat tttctaccca
     2821 gtttgccact ggtaattgat agttcaatag attattcatg ttcactcacc tcgcttgttt
     2881 ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc ttttaatttt ccatcctcta
     2941 caactgcaag tggagtcggt gagtcataaa taatattgaa aatatcactt accaccatgt
     3001 ctttcccaat caccgtcaca ttttcatcta ggaagtcgat caatggacgt ttttgcttcc
     3061 tagcttctaa cgcttgatct gcagtgatga tccctttcag gtgtctcttt ctgtcaacag
     3121 ccatcagcat gctgacttct tcattacgca tccttgtcaa tgcaacgttt ggtccatcag
     3181 attcgatatt cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc actttggaac
     3241 gatcgacttc ttccacaaat tcacgtacat aatcatttgc tggattggtc aatatttctt
     3301 cacctgttcc gatctgcatg atttcgccgt ctttcatcaa ggcgattctg tccccaatcc
     3361 gtaaagcttc attcaaatca tgggtaatga aaataatcgt tttttgaaca ttggcttgca
     3421 ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc gagaacgctt
     3481 cgtccatcag caagatctca ggatcatttg ctaaagctct ggctaggcca acccgttgtt
     3541 gcatcccacc agataactga ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt
     3601 ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg tacaccacgg acttctaacc
     3661 cgtattctgt attttccaag atcgttcgat gtggaaacag accgaagttt tggaaaacca
     3721 tattgatttt atgacgacgg acttctcgca attcttcttt gcttaacttc gatacatctt
     3781 ctccatcaat atagatactg cccgaagttg gctcaatcag acgattcaat agacggatca
     3841 atgtggattt cccactcccg gaaagcccca tgatcacgaa gatttctcct tcttttacgt
     3901 caaaattcac gtcatacaca ccaaccgttg caccggtttt ctttaaaatc tccgttttcg
     3961 gctggtgatc attcatcata tctaaagcgg cttgtgtctt tttaccaaat atctttgtta
     4021 aatgttttac ttctactttt accaaacaaa ttctcctttt ttatgtaaat tcatttttct
     4081 ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt cactttgtca aaaatgaaag
     4141 aaatttgctt gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa aaggtttagc
     4201 caagttaata tttttttaat atttcacaca atcattctgc aagctgatgc tccgggtaaa
     4261 aaaagtttgt catacgctgt agtgtatatt catttccaat aaaatgaacg atgtcccctg
     4321 ccgtaaattt agcatagggg ccaggtgaaa caagcagttc ttcattatga gaaattgcga
     4381 taactgtggc gccagtcgcc tgccagatat tcaactcgct aattgaacgg tccagatgca
     4441 ttgctttttc tgataaagat aattcaaacg gaagtaaagg attcatctta tgtattcgct
     4501 tcgtttgatt gaccagctga tccaataatt gagaaaaatg attcagctca tcttgttgct
     4561 tctccacact tgccatgatt tctgcttttg tatcttggat ggattgtaca tcttgatatt
     4621 gcaataggaa atcttttgct ttttccttcg aagcaacaaa tgccccactt ccatgccgta
     4681 cttccatgat tccaagatct accagcacat tgatagcctt gcgggtagtc tcaggagaag
     4741 cattgtacgt actggcaagt gttgagcggg catggatctt ctcacccact ttatatcggt
     4801 tttcaacgat acgttcggct aaatcaacag caatctgctg gtagcgcggg cggacgattt
     4861 ttttctttcc agacattttc tttcctcttt ctttttttac tttccctcat cttactatgc
     4921 tctagcaaaa taagcaaaaa aggggagaat agcctcttgc aacgactgat catcgcttga
     4981 tctgttcctt ttttactggg caagctgttt gacagtcatc acaattctta ccggatttta
     5041 aacgagtata gacaattgcg cctgcacttc caaagattaa cacacttaat aagaatgtcg
     5101 ccatcctatt cctctccttc taatgacaat atagtataaa actggttctt ggattttggc
     5161 tgacggaaca aaacataaac gagtcctatc atcagtaaaa tagcaagata cgtccctaac
     5221 gctacctgtc caccctccaa taagtgtccc aactgataaa tcacaaaact aactaaataa
     5281 gctaaaccgc attgatagcc tatggcgatc caagtccatt tcttggcatt catttcccga
     5341 tggatagccc caattgccgc aaaacaaggc gcacaaagta aattgaaggc taacaatgaa
     5401 taagctgctg cgggagtata tgtgtgttgt agcacttgcc agatttctct gccattttcg
     5461 gatacttccg catgaccaaa caaaatacca aatgtgccaa taatattttc tttagcgacc
     5521 aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg ctgaaaaaca
     5581 actgccagga ttcgcccgaa aaaggctaag atactttggt cttcagaaac aggttgagca
     5641 taaaaattat aagaagagct gaaccagatc acgatactag aaacaaagat aatcgttcct
     5701 gcttttttaa caaaagactt gctgcggtca taggtttgtc ttaggaccgc tgaccattga
     5761 ggaaaatgat atgcgggcaa ttccatgata aaaggagctg tatctccagc aaacaaacgt
     5821 gtttttttca agctgattcc agagaaaata attgcagtca taccaagaaa ataggcagaa
     5881 ggagctaccc aagattgatg aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac
     5941 tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt
     6001 tcaatcgtgc gactggccat gacgcctggc actccacatc ctgtagcaat cagcatagga
     6061 atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc gatccatgac aaaagcgatc
     6121 ctcgccatat atccgcagtc ttccagaaag ctcaagcata aaaacaatat aatcaattgc
     6181 ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc cgttcaagat cagctcctgc
     6241 atccatcccg ctacttgcca ttcagctaac aatctgccaa catgttctgg taaccatgta
     6301 ccaaacaatg tgtcattgat ccaatctgtc cccattgttc ctactgtttg aatagccaga
     6361 taatagatca gccacatcac aaatgcgaag atcggcaagg ctaaccaacg attcgttgca
     6421 atctgatcga ttttgtcact catcgataat tggaaagaat catttgacac aacacagagt
     6481 gtacatagtt gggtaataaa ttcataacgt tcattgacca gaatcgtctc actatcgtcg
     6541 tccaatagtt tctctgttaa ctggatcagt tgctcgatct ctttttgctg agaaactgct
     6601 atctcgtaat cctctcttac cttctgatct cgttcaaaat atttcattgc ataccagcgt
     6661 ttcttgccgc tttgttctcc tagtacatct tcaatttcat gtaaagctgc ttctaaacgt
     6721 ggatcatatg tacagggaac aggaatagaa ggccgctttt ttgcagactc ctgaatcttt
     6781 tcgattagtt tttcgattcc ttgctttttc aatacgctga ttggtaaaac tggtacacca
     6841 agaccatagg aaagcttttc tagattgatt ttttttcctt cttttttgat gacatccatc
     6901 atattcagac caatgatcat aggaatgtct aaatccaata actgcgtagt taaataaaga
     6961 tttcgttcta aattggtagc atcaataatg ttgatgatca gttcaggctg ttcctgaacc
     7021 aaataatctc ttgcaatcac ttcttctggt gtataaggag acaaagaata gatgccgggt
     7081 aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc cttctttttt ttctactgtt
     7141 acgcccggcc aattccctac atattgattc gaaccagtca agagattgaa caagctagtt
     7201 ttcccgctgt ttggatttcc gactaacgcg atattcattc tttttcctcc ctttgtatca
     7261 agattagttc ggcctctgac tttcgtaacg tcagttcata ccctcgcact gtcagttcga
     7321 ttggatctcc cagaggggct agttttcgga taactatttt tacatttcgc gtcaatccca
     7381 tatccattaa ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg atcccagtct
     7441 ctcctgcttt taattgatca agagaagtcc agctttcaga ggaagcagtc tttttattga
     7501 caaagatttg acctagtatt tcccgactta ctgccactcg atgacttttt accagtaaaa
     7561 tggcattgtt gcctgataaa ttgacgacga cgattttact tccggcaacg atcccgatat
     7621 tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg ttcgacttga taaacgaaat
     7681 tctgctttgc ctgcgctaag gtctgcatat tgattcctct cttgtgaaat aaatcatgta
     7741 ttaaaatcat tcattatatg ttctaatttt cttacgtgtt acgcaaataa atgagaatca
     7801 ttctcacata gaatactctc ggtaattgag aatgtcaatt atacttgtga tattatgagc
     7861 gaatctttag tattgataga taaaaatgag aaaaaaatat ttcttcatag ttttccacaa
     7921 ttttttctct attaaatttg ctattttccc acacgatact tgttttcagc tcttttttca
     7981 aaaacacaaa acttagtatt ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg
     8041 acttttttac taagagtttt atactatcta ttatagataa taagaatgtg ttagaggagg
     8101 cactcaccat gaacgtaaaa atcttttcta aaaataattg tatgcaatgt aagatggtca
     8161 aacggttttt aacagaaaat caaattgctt ttgaagaagt caatatcgat gaacagcctg
     8221 aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt acttctgatg
     8281 cagaaacagt tatcggattc cgtcctgatc agttgcgtaa acttgccagc taacatgact
     8341 aaaagaatca aaacgtgata catggatttg aagggcttga gacagcacca gtttttgtcg
     8401 caaacttcca aatccttttt taaagcttaa aaacaatgca atgatttgca atcaagcatc
     8461 atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac aagatgtcag
     8521 ttatttcaag ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa
     8581 ggataaagaa gctttagttg ccttttttga ggaaaatgtc aaaccaaata cgatgacttt
     8641 tccatctatt atggaaaaaa tcaattactt gatcgatcaa gaatatatag aagcagaatt
     8701 cattcaattg tattcaccat catttattga aaagctatat caatttttgg acgagcaaca
     8761 tttttctttc aaatctttta tggctgctta caaattttat tctcaatatg cgttgaaaac
     8821 caacgacggt tccgcttatc tggaaactta tgaagatcga gtagccttca atgcgcttta
     8881 ctttgcgaac ggggatgaag aattagcact tgctcttgca gacgaaatga tccatcaacg
     8941 atatcagcca gctacacctt ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt
     9001 gtcttgtttc ttgactcaag tcactgacga tatgaactct atcgggcgct caatcaactc
     9061 tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt actttatcaa atcttcgtga
     9121 agccggcgct cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa
     9181 attattcgaa gatagtttca gctattctaa tcaattaggt cagcgtcaag gagctggcgt
     9241 ggtttatttg aacgttttcc atccagacat tattgctttc ctttcagcaa aaaaagaaaa
     9301 tgcagatgaa aagattcgcg taaaaactct ttctcttggc gtgatcgtac ctgataaatt
     9361 ttatgaattg acacgtaaaa acgaagatat gtatttattt agtccatata gtgtcgaaaa
     9421 agaatatggt gtcccattct cttatgtaaa tattacagct gagtatgatc atctagttgc
     9481 aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa
     9541 attacaacaa gaatctggct atccttacgt aatcaatatt gatacagcga accgcgagaa
     9601 tccaatcgat ggcaagatca tcatgagtaa tttatgttca gagattttac aagttcaaac
     9661 gccttctgtg attaacggca aacaggaata tgaagtatta ggaacagata tctcatgtaa
     9721 cttaggctcg acaaatatcg tgaatttgat gcaaagccct aactttggtc aatctgtgcg
     9781 tacgatgact cgtgcattga catttattac agatgcatct gatatcgatg tcgtaccacc
     9841 gatccaaaac ggaaataagc tgaatcatac gattggatta ggtgccatgg gcttacacac
     9901 ttatcttgca aaagaacaaa tggaatatgg ctcagaggat tcattagact ttacaaatat
     9961 ttacttcatg ctgttgaatt attggacact tgttgaaagc aacaatatcg ctcgtgaaag
    10021 aaaacaagtt ttccataatt ttgaaaaatc agcttatgct gacggcactt atttcgaaaa
    10081 atacgtaact ggagaattcc agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat
    10141 ttttatccca actgctgaag attggaatgc tttgaaacaa gcagtaatga aagatggact
    10201 gtatcatcaa aatcgcttgg cagtagcacc aaatggctca atttcatata tcaacgatac
    10261 gagtgcaagt attcatccta tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg
    10321 taaaatttat tacccagcgc cttatctatc caatgagacg attccgtatt acacttctgc
    10381 ttatgatatg gacatgcgta aagtaatcga tgtttatgca acggcacaac aacacgtcga
    10441 tcaaggcatg agtttgactt tattcatgcg ttctgaaatc ccagaaggca tttatgaatg
    10501 gaagacaaca acgaagcaaa cgacacgtga tttgaatatt ttaagacatt acgctttcca
    10561 caaaggaatc aaatccattt attacgtacg tacctttact gatgatgcag aagaaatcgg
    10621 aagcaatcaa tgtgaaagct gcgtgatcta atatcataga tagattcgct tattcatatt
    10681 ttaattaaag gagcattctc atgtcagaaa catattatga agcaataaac tggaatgaaa
    10741 ttgaagatat cattgataaa tccacttggg agaagctgac agagcaattt tggctagata
    10801 cacgtatccc actttcaaat gacctagatg attggcgtac attgtctcaa ttggaaaaag
    10861 atacagtagg acatgtattt ggcggtttga cactgctaga tactgtccaa tctgaaagtg
    10921 gcatggatca gttgcgtaaa gatgtccgca ctccccatga agaagccgtt ttgaacaata
    10981 ttcaattcat ggaatccgtt catgccaaaa gttattcttc tatttttagt acactgaata
    11041 cgaaaaaaga aatcgaagaa atttttgact ggacaaatac gaatccttat ctgcaaaaaa
    11101 aagctgagcg tatcaatgaa atctacaaac acggtacacc gctagaaaag aaaattgcca
    11161 gcgtcttttt agaaacattc ttgttctatt caggttttta tactcctctt tattatttag
    11221 gaaacaacaa attggctaac gtagcagaga tcatcaaact aatcatccga gatgaatctg
    11281 tacatggaac ctacatcggt tacaaattcc aattaggatt caacgagtta cctgaagcag
    11341 aacaagaaac gttaaaagac tggatgtaca atcttcttta tgagctttac gaaaacgaag
    11401 aacgttatac agaagaatta tacgatccaa tcggctggac agaagaagtc aagacattcc
    11461 tacgctataa tgccaacaaa gcattgatga acctagggat ggatccactc tttccagata
    11521 ctgccaatga tgtcaaccca atcgtaatga acgggatttc aacaggcaca agcaaccatg
    11581 acttcttctc acaagtcgga aacggctatt tgttaggtca tgtagaagcg atgaaagacg
    11641 acgattattt gattggattg gaataaggat ttattttaat tcaaaaattt ttcgaaaaat
    11701 aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt atgcttattc
    11761 acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt ccattcttct
    11821 caataaatag attttccgga tatttaatat tatcgtctta atcactgcat agacaggtat
    11881 gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata ccaagatgat
    11941 cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca aatttccatc
    12001 taattgttgg atcactaaaa tcgacaacgc catatacaaa gcttgaaatg gagagataaa
    12061 caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg ggataatatt
    12121 cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata ttctcattgc
    12181 caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga tataggcaga
    12241 taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa
    12301 gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata gcaaaataaa
    12361 tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt ttgagacact
    12421 tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat ttgctgatgc
    12481 taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt catttaacca
    12541 tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg tctgttccat
    12601 taactgaggc actacattca tgacagttaa aacaactaaa atgattacga ccaaaaacgt
    12661 caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa cgattggatc
    12721 gaacatataa tagataaaac cagcaacaag caatggtaaa aaaattgccg aaagcatttt
    12781 caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga tgatcgtgag
    12841 tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt gcatacatct
    12901 cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg cgctgagatt
    12961 ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga cgagtactta
    13021 aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt ttccaaataa
    13081 acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac
    13141 tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca acctcagatc
    13201 atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac ggacaactcc
    13261 tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat atttttgtac
    13321 actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac gatatttttc
    13381 tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa ttggatttct
    13441 aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc aatagcaatc
    13501 ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat ccctaatcca
    13561 gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg
    13621 atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag ccttgcttca
    13681 tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa tgcaccacta
    13741 gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa aatatgcgta
    13801 cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc gccaaatagc
    13861 atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc attcatctaa
    13921 acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt gcaattttta
    13981 cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta tgaataaacg
    14041 gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg ggagctattt
    14101 ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttagcctac
    14161 tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg
    14221 attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca tccctttgat
    14281 tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt tcataattga
    14341 accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga agaatcgttt
    14401 gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt caaatgaagc
    14461 accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt ggatatcgat
    14521 tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat cttctgcttt
    14581 tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga catccatgaa
    14641 atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta agtttacaga
    14701 agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag taactggtgt
    14761 gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt tgatgatttc
    14821 aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca tagtgattgc
    14881 tcctttatat tttggtatag cccaattata gcattctttt tttcttttta caataattag
    14941 tatagaccaa attgcatttt tttcattttc ttgacaataa ataggaatcc gtgttaggtt
    15001 tcataagatg gttcaatcaa acaatccatg ttgcatgaaa accaagcaag tgattcactg
    15061 tggacgcttt atgtctgcag tttttttatg aaaaaggagt tattatgagt atactgacat
    15121 taaacaacat tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat
    15181 taaatgctgg cgaacatctt gggctgattg gacaaaacgg agccggtaaa agtacattga
    15241 ttaaaatcat cactggtgag atcttaccag atgacggaca gatccattgg caaaaaaata
    15301 ttcacaaagg ttacctcgat caatatgtgg aagtcgatga aacattaaca atcgaagaat
    15361 ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct tttctttacc
    15421 aaacatatag cgagaccttc gatgattcct tgctggaaaa agctgggaaa ctccaaacgg
    15481 aattagacca aggagtgttt tatcaaatcg acaccttagt tgctgagatg agcagcgggt
    15541 tggggatcga tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt
    15601 caaaggtgat tttagcaaaa ctacttttag aaaacccaga tgttctgatc ttagatgaac
    15661 caaccaatca cttagatgat cagcatatcc aatggctgac gcagtttctt caggattttg
    15721 acggtgcgta tatcgtgatt tcccatgacc aagaatttct tgatcagatc accacccata
    15781 tcgcagacat cgagtttgga aagattacta agtatactgg ccatctaaaa caagcattga
    15841 aacaaaagga acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa
    15901 tcgaaaagac cgaagcttat attcgaaaat acaaagccgg ttcccgatca acgatggcaa
    15961 aaagccgaca aaaacaatta gataaaatcg aacggctgac gcctccagct tcagcaagta
    16021 aagctgtctt tgattttcca ttctcgccta ttgtcacgac attagcagtg gaaacaactg
    16081 atttagtcat cggatatgat cgaccgctac ttgcacccat caatttgacc atccgctttg
    16141 gtgaaaatat tgccattcga ggcttcaacg ggattggaaa atctacttta ttaaaaacac
    16201 tgatcggtga gattgaaaaa ataagtggcg atttccactt cccagataac acaaaaatca
    16261 attacttttc acaagatctg tactgggaaa atccattaga aacaccacta caatatttaa
    16321 gcagtcaatt tccaaaagcg actatcaaag aattacgtcg ccaactagct aaagcagggt
    16381 tagttaatca attagcaagt gaacctctca ctacattaag tggtggggaa caaactaagg
    16441 tgaaacttgc acagttgaca atgaaccaag gtaatttact gattctcgat gaaccgacga
    16501 accatatcga tcaagaaacg aaagaaagct tacaagacgg gatacagaaa tttccaggaa
    16561 cagtaatcat cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg
    16621 agattgaacc aaaataaacg tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt
    16681 tttgtcacaa ctccttcttc tgaataaatg gtggccaatt agctgacctt cggtattagc
    16741 tcaaaaaacg gcaaaatatg aggagctatt tttatttggc tattcttatt acttgatgca
    16801 gaacagcttt tcacaacctc ttttcgtcta ttttttattt tcgagatttc tctttccttt
    16861 aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc taaccgttac
    16921 acctaaccag ccatactgct gccacattgt cgtccctagc aatgaaccag ccgcacctcc
    16981 aataaagtac ataaacataa ataca
//
LOCUS       DASHRQ010000057        16994 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.060, whole genome shotgun sequence.
ACCESSION   DASHRQ010000057 DASHRQ010000000
VERSION     DASHRQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.060"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(266..1633)
                     /gene="nhaC"
                     /locus_tag="U2M88_002089"
     CDS             complement(266..1633)
                     /gene="nhaC"
                     /locus_tag="U2M88_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEM8374449.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1742..3163)
                     /locus_tag="U2M88_002090"
     CDS             complement(1742..3163)
                     /locus_tag="U2M88_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8374450.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3406..5283)
                     /gene="tdc"
                     /locus_tag="U2M88_002091"
     CDS             complement(3406..5283)
                     /gene="tdc"
                     /locus_tag="U2M88_002091"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEM8374451.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5574..6830)
                     /locus_tag="U2M88_002092"
     CDS             complement(5574..6830)
                     /locus_tag="U2M88_002092"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEM8374452.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(6942..7147)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7410..8711
                     /locus_tag="U2M88_002093"
     CDS             7410..8711
                     /locus_tag="U2M88_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEM8374453.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8780..9379)
                     /locus_tag="U2M88_002094"
     CDS             complement(8780..9379)
                     /locus_tag="U2M88_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM8374454.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9633..10514
                     /locus_tag="U2M88_002095"
     CDS             9633..10514
                     /locus_tag="U2M88_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8374455.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10527..12137
                     /locus_tag="U2M88_002096"
     CDS             10527..12137
                     /locus_tag="U2M88_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEM8374456.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12214..12873)
                     /locus_tag="U2M88_002097"
     CDS             complement(12214..12873)
                     /locus_tag="U2M88_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEM8374457.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(13156..13821)
                     /locus_tag="U2M88_002098"
     CDS             complement(13156..13821)
                     /locus_tag="U2M88_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEM8374458.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13968..14573)
                     /gene="dhaL"
                     /locus_tag="U2M88_002099"
     CDS             complement(13968..14573)
                     /gene="dhaL"
                     /locus_tag="U2M88_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEM8374459.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14601..15611)
                     /gene="dhaK"
                     /locus_tag="U2M88_002100"
     CDS             complement(14601..15611)
                     /gene="dhaK"
                     /locus_tag="U2M88_002100"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEM8374460.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(15586..16032)
                     /gene="dhaM"
                     /locus_tag="U2M88_002101"
     CDS             complement(15586..16032)
                     /gene="dhaM"
                     /locus_tag="U2M88_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEM8374461.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            complement(16071..16943)
                     /locus_tag="U2M88_002102"
     CDS             complement(16071..16943)
                     /locus_tag="U2M88_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM8374462.1"
                     /translation="MVQRSRQTIYNVYRFLKSGGSAISYYEQYKKNKRNCGRRPIVLP
                     DGQQEYIQKKVAQGWTPDVLIGRAEFPIACSVRTLYRMFKDKTFDPHDLPMKGKRKPN
                     GHKEKRGKQAFRRSIQDRTTDYIQFNHEFGHLEGDTIVGGKHKSAVITLVERLSKVII
                     TLKPAGRQAIDIENRLNQWFQSVPKHLFKSITFDCGKEFSNWKKISNTNDISIYFADP
                     GTPSQRGLNEHSNGLLRKDGLYKAMDFNKVSETFIQSVASKRNHIPRKSLHYRTPLEV
                     FLSYVGKDDLSSLI"
BASE COUNT         4871 a         3532 c         3079 g         5512 t
ORIGIN      
        1 aatacgctga ccatttatcc tatggtcagc tttttatgta aaagaagata ctacttctaa
       61 cacattgttt atctgtaaaa aagagcgtgg gacaaaagta aagaacactt ttgtctcacg
      121 ctctaaacac gtataaacgg cggaagcaga agcaactcct tcggaaataa gctgaaattc
      181 acaaaaattt aaaaagcaat tttcttgagt ttcttcttat ttcttggagt taaacgcttc
      241 tgtcccagcc tcttttttcc ttccttcaat tttttacacg tcgacgattc aataaaaaac
      301 cgcctaatat tgtaaagaca ggtgcaagaa tcgagaaaaa ggcgaatggc aagtaatgca
      361 acgcgctaac tttcatcgtt cccataatga atgtgccgct gactccccac ggtacgagag
      421 aattgacggt cgcacctgaa tccgctagtg ttcgtgtcaa ataggttttt gataagccaa
      481 cgtcatcaaa agaacgcttg aatgtttctc ctggaagaat gatggacaga tactgttctc
      541 caacgatcag attgattccg atacagctca aggcagtaaa cccgactaaa cgagaaggtc
      601 ggtccatctt ttcttttaat tcttgaacga tggtatcaac gatcagatat ttgatcagta
      661 acccacctaa tgccaatgca agtaaaatca aactagcaga acctaacata ctcatgatcc
      721 cgcctcgaga aagcagttca tctagctttg gatcaggtgt atttgcgaca taaccattca
      781 tcaagatgtc tgctgctttt ccgaccgtca aacttttctc atggataaaa gaaagaacga
      841 gagcagctgt cgatcccgct agtaatgtag ggatagccgg aatttttttg attgcccccc
      901 caaataaaat aactagtgga atcaatgtcc aaggagagat ccaaaagctc gctttcatgt
      961 tttggatcat ttcattgata ctagctagat cggctgaatg actgttatgt cctaatatcg
     1021 tataaaaaat caacgaaagg acgaatgctg ggaaatccgt ataacgcata ccagcgatat
     1081 gttcgaatag attaactcct ccaatacccg ctgccaagtt ggtcgtatct gataaaggcg
     1141 agcagttatt ccctaaaaag gcaccggaaa cgactgcgcc tgtagtcatt gcatcattga
     1201 atccaagtaa atgaccgatc cccaaaaaag caattccaat cgtcgaaatc gttgtaaatg
     1261 aactgccgac agtcactccg acaattgcac aaacaagaaa tgctgtcggt aaaaagaaac
     1321 gcacagaaac aagtttaaat ccataaacca taatcgtagg gatggttcca gataaaatcc
     1381 atgaagcaac taagacacca atcaaaagaa aaatcaggat tggaatgatc cctggagtga
     1441 ttcctttgat gatcccgtca tggatctcat cccatgtgaa ctttttaaat ctgccataaa
     1501 acaacaaaca catgaaagct gcaaggatcg ggatatgtgg cgagagctgg aatccgatga
     1561 tcagtactcc taggagtgcc aacaacacga tcaatacagc gatactttct tttaaagaaa
     1621 tatttttatt catcgtcgat ctcctatttc aaatgaaaag gccctgccat gaaaaaacta
     1681 ttcacttggc aggacctcct actaaatcag cgacaaaacg tcggctgaaa gacttatttt
     1741 tttatttttc ttctaaactt ttttctacct caatccccca agaatctttc ttgaagtgat
     1801 tgatgatcaa tggaataacg aacatgacca ctgtgatagc tgctactaat aatgcatcac
     1861 tcaatgtcgc tgtacctaat gtagcaaata cgacaacgat gatagagaag atcagcatgc
     1921 agctgactac ccaagctaat ccgtttccgt ttttaccaat tcggtaagga cgttcgaatt
     1981 ctggatgttt cttacgcatt ttaagcaatg aaacagcaat caaaatgtac acgatacaat
     2041 aaataacagt tgtagcattt gtaagtgtca agaatacagc acttacgtca tccattacac
     2101 cataaagcaa agcaaataat gaaataacga ttgattgtgt tagaacgaca tttctagata
     2161 cgccgtattt gttttcttta tggaatccga attttggtgg aaggaatcct tcacgagcta
     2221 cttggatgat tgtttttgat ggtccagtaa cccaagctga taattgtaaa agcacaccaa
     2281 taaataccat gaagctgaag atatttccga tcacttctgg taagcctagg atttgacagt
     2341 caatcaagat aggctgcgtg atatttgcta attccatttt tccatctggt acgacatttg
     2401 ctacaagcat cgcattgatg acattcaata aaactaatcc gatcaaagcg ataaatacgc
     2461 cttttgtata attttttgtt gcgtctttca aacgaggcat gtaaacagaa ctcatttcga
     2521 tcccaacaaa aataaatgtg ataccagcta agtatttcaa tgaatcaata tgttccaagt
     2581 cagggaaagc ttttgaccat gaaaagtcac ctagatagcc gtttgagact agtccgactt
     2641 tgaaggctgc caaaacacct aaaacgaaca tgatgactac tgggatatat acccctagcc
     2701 atacaccgat gttcccgccg acttttgcca tatcgaattt caagttcaag atcgtgatga
     2761 tccagtagat aactaaaata caacctaaaa cgaaccagtg attattgcca agttctacat
     2821 taccaaatgt gtttcctaac aatggtccta aagtagaagc gaccattacc atacctggga
     2881 acatttgcac ccataatagc catgcaacga caaatcccca tttgctgccc agtgctgttt
     2941 ttacccataa ctgaggaccg ccttcttgag gaagcatcgt ggaaagctca ccggaaacca
     3001 ttgatatcgg accggcaaaa aacaaaactg caaaaattga ataaaaaatc aatgtccaac
     3061 cgactgcggc taacgtagga atactccgaa ccgttgcaca cagtgccatt gtcagaccga
     3121 taaaggtccc aagacccatt ttacttactt cagatttact caccgttgac actctccttg
     3181 attttttgga taaaataacg gtgtctttga agtagtttca gcaataaacg aaacaattca
     3241 gcgaatattt gaggggattc tttgagttta ttgcttgaag ggtttcttca aagacaacct
     3301 caaaacaaag ggatcgaaga gcttaggtct ccccttgctc ttcagatcca aacatgcgtc
     3361 agaaacagtt agtattcaag ctgttttcta acatgattac cttgcttatt ttgcttcgct
     3421 tgccaataat tggtctgcgt agattttttc caatttttct tgaagagctg ctttgatttt
     3481 ttcagcatat tcttcaaagt gttcttcttt gttcatgtat ggtgtcatta cgctggcacg
     3541 caatacagtt acttttccgg cacggttcca ttcttcatct gagaatccta gttggttcac
     3601 aaattgtaat gggctgttgc catagtctgg gatagcaaag tcagtatgtg aagttaagaa
     3661 ctcattaccg taaatgcttc ctttcacgta tgaagaataa tcatatacgt catggttcaa
     3721 tttattcatt gcaactaagt catcgttgcc tttttctttg aatacatagt ctaccatatt
     3781 gaaatctgga taagttaatg gatgaacttc gatttctttg tctcctactt taaatgacaa
     3841 gtcattcaag aagttgtaga aacgatgtgc accttcgatt gatgcaccca ttaatttacc
     3901 atatcctgtg acattcaaag gtaagacatg gtgtgctgcc caaacgctgg ctgctgttgc
     3961 ccctgctttt gaaccttcca agatataagc cccaagtaat gctgggatat ctgcgccttt
     4021 ttcaaataca tatgtcgcaa agtaagagat aacgtctctc atgcggatat ctttgatgac
     4081 gataccaccg gcagaatatg gaacatagcc cattttatgt gggtcaatcg ttacagattc
     4141 tgcttcttcg attgctttat atgcactgtg tacttcttct aagatataat ctttgttttc
     4201 tgtaaagaca ttgtatttgt aatgaacatc ttttaaatct tcaaatggaa tgaagttgtt
     4261 gtcttcgtct aagaaaatcg cacggccgta tccgccataa gcagcatcta cgtgtaagta
     4321 gaagtaaata ccgtcttttt ctaatacgcg acgcaatgca acgattttat cgataccgtc
     4381 gattgctcct tcttctgttg atcctacaac accgactaca cctaggattg gtgttttttc
     4441 agctgctaga ccacgaacga ttttttcaag ttcgttgatg tccatgcgat agttgtggtc
     4501 aactggcact ggaatcactt ggtctaaacc gataccgatg atgtcggcag cttttagcca
     4561 tgaatagtgt ttagtttgtg gaaccaacca ttttcctaat ttttctaaat gttttccgct
     4621 acgtgctgag tgagctttga tttcgtcgat tttttctggg acgctgtcta acagattcat
     4681 gatttcttct gttgacaagt tcattaattc ccaatcgctt tttccagcaa ctagttcagg
     4741 tgtcacttct ttcattgcaa gcggcaatga tttgatgttg cgggcatacc aaagtccttc
     4801 taagtttgct aaagaaccat cagcaacgat gtggccccag ccatctttat agctcattaa
     4861 ttttgcaaat tccattccta cttcttcttc catttggctt gttgcaggag aagattcata
     4921 ggcaacgtta ttaccattcc atagcatagc aaaattgtac gctagtaaag atggcatcaa
     4981 tgtttcagag ttcatatgac cccagtaacg tccggcatta tgccaaggaa cggaatgggt
     5041 acgcatccgc gctgaaattt ctgatagaac atcctttgtt ttattcacag tgtgttcaaa
     5101 actagcactg cttttttctt ctggcgtaat aataggcatg tcttgaggca tgtagttttg
     5161 acgccagcct aaatgttcat ccaccaattc attcaacaaa gctttataga tttggccgtt
     5221 ctcagctttg tccccaatga atagggcatt taggtttaga tctttcgaca atgattcact
     5281 catagtgaaa ccctcctaaa aaaatattta aattttgtcg agccctacat tacacctgtt
     5341 cttctattct gtaaactaag ttttggcctg atttttaatc atattttaat atgtatcaat
     5401 tatccgaatt gttcacactt ttgaaataat taaaaaaaac gagggaattt acaaatgtaa
     5461 accccctcgc aaagattgta ttaacgacaa ccagcgttct attttaggat gggtttcaaa
     5521 attggaatgt tgtaatagta tttccctttt tcaaggtaaa aacttactaa attctaatca
     5581 attactttgg ccaaaaagta atttttctta ccttttcgaa taaccacaaa cttaccatcg
     5641 aattcatcgg atggatttat gacaaaattt aaatcggtaa cgcggtcacc gttaatactt
     5701 atagctccgt ttgaaacatc ttcacgtgct tgacgtttag atggttcaat ttttgtggaa
     5761 acaagcagtt ccacgatatt ttcaggtgtg cttgaaattt caacgttcgg cattttaccg
     5821 aatccttgcg cgatttcttc cgcatttagt tctttaatat ttccagagaa caatgcttgc
     5881 gttatttttt gcgcttcttt caagtcttct tcactatgca caaatcttgt aacttcttct
     5941 gccaaacggc gttgtgcttc acgtttctca ggttctgtct ctactttttt cgctaagtct
     6001 tcgatttctt cttgagacaa gaaagtaaag aatttcaagt atttcactac atcgcgatca
     6061 tcttgattca accagaattg gtagaactca aatggtgaag ttttcttcgg atcaagccaa
     6121 acggcaccgc ctgcagtttt tccgaatttc gttccgtcag ccttcagcat caatgggatc
     6181 gttaaaccga acgcttctgc ttctggtcct tctaattttc ggattaggtc taatccagct
     6241 gtgatatttc cccattggtc tgctccaccg atttgcaatt gcacatcgta tgttttatgc
     6301 agatgcaaaa agtcgataga ttgcaggatt tggtaagtaa actctgtaaa tgagattcct
     6361 acttccaaac ggctggcaac gatatctttt gctaacatcg tgttgatgtt gaagttttta
     6421 ccgtagtctc tcaaaaattc aagtaaagag atttttgaca accagtcgta gttgttcaca
     6481 aatgtgatat ttgcatcttt accaaacaat tttctcattt gattggaaag tgcgtccacg
     6541 ttatgttgca cttgttccat cgtttgcaac acacgttctg ttttgcggcc gcttgggtcg
     6601 ccgatcgttc ctgttccgcc gccaatcaga atatatggat gatggcctgc caattggaat
     6661 cttttcatca tcataaatgg aatcaaatgt ccgatatgca tcgaatcacc agttggatca
     6721 acgccgcaat aaagtgaaat gctcttttct tcaacgagct cgcgaagtct ttcttcgttt
     6781 gtttgctgat taatggcgtc gcgccaagtt aattcatcaa taatattcat tttactgacc
     6841 tccgtgtagt ttttttatga aagcaggaac ctgctagcat gtgtaattaa ttatttcttc
     6901 aggataggat aggaaataaa aaaagtccct tggcatataa gccaagggac gaaatctcgc
     6961 ggtaccaccc aatttgtatg tctagatatc caacatacct ctcgaacatt taacgctgtc
     7021 gcgtggcaat cactgccaaa gactcctgaa atgtaattcg ctttaaagca tgtactggtt
     7081 tgcagcgacc accagctttc tgaaacattg gctttaacaa ctacttcgtt tcgatcttcg
     7141 tcttgttatt attaatatgt gtctatcata gagcgcaact ttttctttgt caacagaaaa
     7201 aggatatttt ttttaaattc ctcatttttt cttttttgaa tccgctctaa ttccgaacat
     7261 tttattttca attattgtta aaagaaataa ctttttctgt attttttctg caatttatcg
     7321 agaagtcact ttactttgcc tcttattctt tcttcgttac aataaggaat gggacaagta
     7381 aaaaaatgaa aaagcaaggg acgaggtata tggaaaaaaa gcaaaaaatt attttaggag
     7441 ccacttctgc tttaactatc gttattttaa ttctaagttt gttttttgtc atccgatcat
     7501 ctacaaaaaa agaaccaatc attcctgaaa aaaaaataac agaaaaagca aaagaaccga
     7561 cagatgataa gcaacaacta gttactcaag caaaaaatct ggctcaaagc tacgattatg
     7621 accaagcgat tgcgttattg gaaaaagata gtaagaacgg atctactgaa acacaacagc
     7681 ttcttcaaac actaaaaaag gagaaagaac aactgatcga ctggtctgat cctactcaga
     7741 tctctcatgt ctttttccac agcttgatcg tggacccgca aaaagccttc cactctcaac
     7801 aggcacaagg atacaaagat tatatggtga ctgttgaaga attcaaccgt tcgatcgaac
     7861 aactttatca aaatgatttt gttttagtta atttgaatga tttgatccaa aaagacgaac
     7921 aaggaaatct ttcctttaac gggcttgcgc tacctgaagg aaaaaaacca ttggttcttt
     7981 cacaagatga tgtcagctat tatgaatata tggataattc cggttttcct agccgattgg
     8041 tcgtatcaaa taacaatgaa atcaaaaatg tctataaaga gaaagataaa gaagaaatcg
     8101 gtgactttga catggttcct ctgatcgatg cctttgtcaa gaaacatcct gatttttctt
     8161 atcgaggcgc aaaaggtacc ttggccctga caggttacaa cggagtttta ggttatcgaa
     8221 catcaaaatc tcaatatgga gacaatgaaa aaacaaaaaa agagattaaa gaagctaaaa
     8281 aagtggcgga acagttgaag aaagacggct ggagctttgc ttcccataca tggggtcact
     8341 tgaatatgac tcaagcttca ctagaagatg taaaaaaaga caatgaattg tggcagcaag
     8401 aagttgcacc tatcctagga aaaacaaata ttctgattta tccatttggt gcagacatca
     8461 gcgactggca gccttattca gcagaaaatc aaaaatttgc ttacttgaag agtcaaggtt
     8521 tcgatatttt ctgtaatgtc gatgcttcta caccagcttg gggacaatta ggtacggatt
     8581 tctatcgaaa tgctcggatc aatatcgatg gcattcgttt tcaatctgat ttagacggaa
     8641 aaaatcctat tcttgatcca ttcatcaatg tccatgatgt ctatgacaaa gaagcacgcg
     8701 aatctgcttg attttacgtc atttatgaac caaaaaagcc gaactgacat tttatcgtca
     8761 gtttcggctt ttttatgctt tatttcttct tcggtgcgat tcccttgatc aggacttctg
     8821 tccattctgc tgtatatgga agcagttttg cgtatatctc cggcttagaa attccttcta
     8881 ccattgcttg aaaatagagg atcaagaact catccgaata tttcaaatcc acttttcctt
     8941 ctttccgccc tcgatggaaa agatcaagca tgatactgaa actttcttgc gtatacgcat
     9001 ccatcgcgtc agaaaattct tttttttctt tttttgtata gtatgtcatc aaatcgatat
     9061 aaaattgttt gttcactcga ttgagataac ggattttgtt ttcactcatg gcaatcagtg
     9121 tttcttcaaa tggaagattt tccgcaatca acgctttcgc ttcttgccct aaatcattca
     9181 taaatgcaaa aaatacttca taaatcagcc cttccttgct gccaaaatat ttaaaaatcg
     9241 tcgctttact tacctgagcg ttctcagcta ccttatccat tgtcactcca gcggttccta
     9301 tgtctgtgtt catcaagtca aaagctgctt gtaaaatctc tttctttttc tcttgtgctc
     9361 tcttttcaaa accgttcatt tgtagagcct cccgtaattt taaactttta taatatatat
     9421 agttcaaatt tattctgagg tttattataa ctatctattg agaaagtatc aagctagcaa
     9481 ttccttgttt atgaattcat tgacagaaat ttataaaaag aatattatgt actaaaataa
     9541 taaaaaaagt tttataaaaa ggtataaaac ctttttatta aactatttaa tcttgtttag
     9601 tatatatttt tagaaaaagg agtggggttt ttatggcaga aatcgtcaaa gtcacacatt
     9661 tgaaaaagag ctttggtcgt ttcgaagctt taaaagatgt ctctttcacc gtacattcag
     9721 gtgaagtcgt tggatttatc ggaccaaatg gttctggaaa atccacgacg attcggacac
     9781 ttttagggat tataaaaaaa gacggcggaa cggcaacgat ttttgggcaa gatgtatggg
     9841 aagatagttt atctatccat gaacgtcttt cttatgttcc aggcgatatc tctttgtggg
     9901 gaaatttgac tggaggagcg atcatcgatc tcttcatgaa gcttcatgga aaagggagtg
     9961 ctcaaaaaaa agatgaactg attcaacgtt tctctcttga tcctaagaaa aaagcaaaaa
    10021 actattcaaa aggaaatcgc caaaaagtgg gcttgattgc tgctttggct gttgattcgg
    10081 atctctattt atttgatgag cctacttctg gtttagatcc tttgatggaa acgattttcc
    10141 aagaagaagt tgaaaaattg aaagaacagg gcaaatccat cctactttct tctcatattc
    10201 tcagtgaagt cgagcgacta gcggataaag tcgtgatcat acgcgaagga acactcgatg
    10261 aagaaggaac actcgatgaa ttgcgtcatt tgactcgttc gaccatcact atagaaacag
    10321 aagcagagaa tagtcactta gcacaattga acggtgtcca tgactttgtg caaaaaggtc
    10381 acctcgctca atttgctgct gataacgaag ctttcgatgt gattttagct gaagcgtcca
    10441 aattaggtgt gaaaaaattt gaatctattc caccgactct tgaggattta ttcatgagac
    10501 attatgaagg atgaggagga agcgaaatga ctgataaatt ttcacgctgg tccacccttt
    10561 tttctcaaaa ccttagccgt gactggaaaa agatcctgat atggctagtc ggtgtcactg
    10621 ctttttctgg tggttttgtc cctgcgtttg aagaattgac ttccggacaa ggattagctg
    10681 gattatacga aaccttgcaa aacccggcaa tgattgcaat ggtcggacca actccagctt
    10741 cgaatgctgc tgagtatact ttaggagcga tgtatgcaaa tgaaatgttg cttttttgca
    10801 gtttgtttgc tgctgtgatc tctgttttac acgtggtgag ccaaacgaga aaaaaagaag
    10861 aacttggtct gattgaactt attcgttcct ttcgagtcgg acgtcaagca aactctttag
    10921 ctgtcatcgc tgaaagtatt ctgatcaatt tgcttttagc aatatcgatc agctgtacga
    10981 tgattttttt ccaagcggat tcgatcactg cagaaggtgc ttggcttttt gggctatctg
    11041 taggtagtat ggggattttg gggagtgccg ttgcactgtt tgctgcacaa ctgatgccaa
    11101 gttcagccag ttcgaatggt ctgtctttag cgtttttagg tggactttac cttctacgag
    11161 caataacaga tgtagctgat tcctcccttt cgatgtggaa tcctttaggc tggtcttatt
    11221 tgacctatcc atttacaact aacaactgga ttcctttgct ttatttgttt ttgttcagtc
    11281 taatcttgat aatcagttca tttgttttag aggaacatcg agatttgaac accggctata
    11341 tccccgaacg tgaaggaaaa ccgtatgcta aacgttctct tttatccttg cgtggtttat
    11401 tattctatct aaataaaggg atgacaatca gttggcttat cacttttggt ttgatggggg
    11461 cggcatacgg ctctatctac gggaatatgc aacagttttt agaaagtaac gacttaatga
    11521 aacagatgtt tatccaatct ggcgtctcaa ttgaaaaaaa ctttacaagc acgatcatgg
    11581 tcgtcttgat ttgtctagct gctattttac caattgctag catcaatcgt ttatttacag
    11641 aagaaaccca cggacgtatg aatcagctgt tcgcaacaaa aatttctagg aaccaacttt
    11701 attggaccaa tatgttacta gctattctat ttgctatatt ggcaatcctg attagttctg
    11761 gtgcactagg gctttctgct ttggcttcga tgaaggatgc gaaagacatg gatctattga
    11821 tgtttcttaa agccgggttc aatttcctgc ctgccgtttt agtgtttatc ggaattagtg
    11881 gctttctatt cggatggctt cctcgaatcg gtaaacttgc ttatatctac ttagcttact
    11941 cctttgcact caattacttt ggcggaattt tggatttacc agaatggttt tccaaaacag
    12001 ctgtccctag ctgggttcca cggatgccaa acgattcttt tgatctttcc gtgttcctta
    12061 cgttatcatg tatcagcctt gcactgttcg tccttggttc tattggttat aggcgaagag
    12121 acttactaga aggataataa aaaagcatgt acaatttcat gctagcctcc aaatctgggt
    12181 caaaaatcca acatttggag gtcatttttt tatttacaag tcttctaact gctctttatc
    12241 aatgatccag acttctcgat tctttctttt tacataacca ttctcctcta ataatttaaa
    12301 ttttctagat aaagtttcgg gggtcgtacc gataaatgca gcaagttctt tcattttcat
    12361 tggcaggaaa aagtgatcct tccctgtcgc tttagctaag tcaagcagat agacagcgac
    12421 tcgttcttct actttttcca tcatcaaaaa agttgtctgc tgctgtactt ttgttaattt
    12481 ctcaacgttg attgctaaaa gtttcagact aagggcggga taatgcagta agagttccga
    12541 gaaatctttt tgtctcaata tgcaaatttc cgttttttct agtgcttcgc caaataaaga
    12601 ctcatttgga acgcctagca actggctctc tccctcatat ccgcccggct ctattactcg
    12661 taaaagctgt tcctttccgc tagcagataa ttgatacact ttcatactgc cggaggccac
    12721 gatcactaat tgaggttctc cattcggcgc aagaatctgt tctccttttt ccacatagtg
    12781 atgtttgaca agtgcgtgta ctttttcttg atcttgctgt actaagtgat tgaacaaagg
    12841 aacaaatgaa acacataaat gatcatgact cataatctct cctccttcta aacagctatt
    12901 tctaataaag aaacataact tcccttattc caactgataa ttttctttta tcatagcata
    12961 aacgtcaagc gaaccagata ccagcaaata gagtggattt ctttttgtcc cttgctttat
    13021 tttccttctt ttgagacctt aagtaattga ttcctatcaa ttttttaaat atttactttt
    13081 tgaataatca aaaacaaacc ttcactcgca agccgagagt aaggtttgtt tttcgcattg
    13141 cttatttcgt gttgtctatt gaaatttttc cgcacctagc ttttgatata gtggtgctag
    13201 ttcttgtaag gtttcagcgg ttatttgctc aacgtgttcg atttcttgcg gatagaagaa
    13261 tagtttaccc atttgaaagg ttcggtctct catcgctttt ttcaactctt cttgtttctt
    13321 tgtatcggac aaatagtcag acaaagctaa gtggtcgtca agttcatgtt ctggttgcgg
    13381 ccatatgtaa taatccttga tatcgactgt tttgctccac tctttcagta tcgcaagcaa
    13441 ctggttatac gctgcaatgc taccatatcg ttcttcgctt ttataatgtt ggtacatcaa
    13501 atagatttgg tattgttttt gattcaacaa aactgctaga caggcattct cgtcttgacg
    13561 agattcgctt cggtacgcgc tccaaaaatg actgcgtaaa ttccagccat tcgtccagct
    13621 ttcgatttta ggtttgctca tgccgtacat atcaggcaaa agtgcagctg tctggagctg
    13681 aatctctttc catttttgcc agtgttcttt atattctttc ttgatttgtt caatcttatc
    13741 tgttggatgg ctctctttga gctgtttgaa tgcgtagatt ggtcgatcaa acaactgata
    13801 atctgatttt ccaagttcca ttgtttcgcc tctttccgtt atcttttgta ttctacctga
    13861 attttgcgat atggacaaat agaggttgtg aaaaagctgt tctgcatcaa gtcataagaa
    13921 cagccaaaca aaaaactgcg atgagcatca ccgcagtttt attgaactta aggttgattg
    13981 atcacatcta aaagcgttga aaataaaatg gcactagaag cagctcctgg gtcgatatgt
    14041 ccgattgcac gctctcctag ataggaagct cgtccttttt tagctaccaa gactttggtg
    14101 tgttcttttg cctggtcaat gatttctttt gttaaattcc ctgccttcaa tgcctctgtg
    14161 actggcagcc aaacatctaa catcgtttta tcgtcagctt ctgcctttcc tcggctttga
    14221 atacccgctg ttccctgctc gatcacttcg cctaattttt cttgagaatc aatagaatcg
    14281 acctcttttg tcgctttcat catgttcatg aatgcagatc cgtataacgg accagaagcc
    14341 ccaccgactt tcgatatcat cgccatagag aatactttga atgtatcgct gattgtttca
    14401 ggatgatcgg cttgaaaagc ttcttcataa gctgaaaccc ctcttcccat gttatttcca
    14461 tggtctccat cgccaatcgg ggtatctaga tcactgagat attgtttgtt ctgattgatt
    14521 gcctctgcaa attgcgtgag ccattcttgg atttcttgaa cggttagttt catattatca
    14581 cccttttatc ttgagatttt ttaccatgaa atcgtctgta cattgctttc taaagaatct
    14641 ttccattgtg tctccgtcaa gtcgatcagt gttagagaaa gaccttgcat atctagtgag
    14701 gtcataaaat taccgacttt tcggaaaact acattgacgc ctctttcttc caacagagct
    14761 aaaacatcat tcatgaacac gaactgttcc atcaaaggcg tcccgcccat tccgttgact
    14821 agtacacctg cttcttttgg taaattcgag taatcatcta aaatcttagt aacaagttcc
    14881 tttgccagta ctttagaagg ctgcatcttt tctcgacgat agcctggctc accgtggata
    14941 ccaacaccat actcgatctc atcttctcct agttcgaatc ctggtttccc tacttctggt
    15001 acagttgctg ctcgaagcgc tagaccgatc gtcttagttg cctgtacgac cttttctccc
    15061 agttctttca actcagctag ggaagcaccg tttcttgctg catcgcccac gattttgtgt
    15121 acaaggacag tacctgcaac tccgcgtttt ccagcagtat acgtactatt ttcaacggcg
    15181 atatcatcat ctacgatcac actttctact tcaatatcgt ccatagcagc taattccttt
    15241 gccatatcga agttcaatgc atcccctgta taattcttaa cgatcagcaa aactcctttt
    15301 ccactatcgg cttccttaat agccgtctga atctgatctg gagttggaga agtaaacaca
    15361 tcaccaagca ccgctgcact taacatccct tcccctacaa acccggcatg ggatggttca
    15421 tgtccgcttc ctccaccaga aaccaaacca acttgttccg ctttctgatt cttggctaca
    15481 acacgggaag attctacacg atgaacaagt tctggatagc tttttactaa tccttccagc
    15541 atttcttcga caatattgct tggatcattc atgatttttt tcaatttatt cccatccttt
    15601 ctttccggca ttttgtgctt ctgttaagat ttgctgagta tcatctgtta taccggcagt
    15661 gatggctgct gcaaaagctc cttccactaa aggcgcatcc accagatgat aacgttgctg
    15721 ctgctcttct tccaacatgt cttttgccag ttccgagttc aataccgcgc taccgatatc
    15781 cgcaaaaaca aaataactag ctgcatcgct tgattcattg actgcttcta cgattttcat
    15841 cgggtctgat ccgatctcgc catttattcc gccgagagaa aaaatttgca cctcttcgga
    15901 tttttgcatc tgctcaatca tttctttaat accatctgtt actttttgac tatgagaaat
    15961 aagcaaaata ctttttttca caagaatctt cctttctttt acatagttgc taacgattgg
    16021 tttcgctttc attaatgaac aattcaagta tataataaat ttttcgtttg tcaaattaag
    16081 ctagacaaat catctttacc tacgtaactc aaaaatactt ccaatggtgt gcgatagtgt
    16141 aatgattttc taggaatatg atttcgtttt gatgccaccg attggataaa ggtttcggat
    16201 accttattaa aatccatagc tttatataag ccatctttac gtaataagcc attcgaatgt
    16261 tcatttagtc ctcgttgaga aggtgttcct ggatcagcga aataaatcga aatatcattg
    16321 gtattactaa tctttttcca attcgagaac tcttttccac aatcaaatgt gatggattta
    16381 aacaagtgtt tcggtacaga ttgaaaccat tgattcagtc tattctcaat atctatagct
    16441 tgcctaccag cgggtttcaa ggtgatgatc actttagata atcgctcaac taacgtgatc
    16501 accgcacttt tatgttttcc accgacaatg gtgtcacctt ctaggtgacc aaactcatga
    16561 ttgaactgta tataatcagt tgttctatcc tgaatcgaac ggcgaaaagc ttgttttcct
    16621 cttttttctt tatgtccgtt tggttttctt ttccctttca ttggtaaatc gtgagggtca
    16681 aaggttttat ctttaaacat ccgatatagt gtacgtaccg aacaagcaat aggaaactct
    16741 gctcgaccaa taaggacatc tggagtccac ccttgagcca cctttttttg aatgtattct
    16801 tgttgcccat ctggtaacac aatcggacgt cttccgcagt tacgtttgtt tttcttatat
    16861 tgttcatagt agcttattgc gcttccacca gatttaagaa aacgatagac attataaatg
    16921 gtttgtcgag aacgttgtac cagatgggct acttttgaaa cagagtgtga ttgattgaaa
    16981 taagcttcta tcat
//
LOCUS       DASHRQ010000058        16852 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.061, whole genome shotgun sequence.
ACCESSION   DASHRQ010000058 DASHRQ010000000
VERSION     DASHRQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.061"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..220)
                     /locus_tag="U2M88_002103"
     CDS             complement(<1..220)
                     /locus_tag="U2M88_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEM8374463.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGN"
     gene            complement(217..510)
                     /locus_tag="U2M88_002104"
     CDS             complement(217..510)
                     /locus_tag="U2M88_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374464.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            complement(547..684)
                     /locus_tag="U2M88_002105"
     CDS             complement(547..684)
                     /locus_tag="U2M88_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEM8374465.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(686..1117)
                     /locus_tag="U2M88_002106"
     CDS             complement(686..1117)
                     /locus_tag="U2M88_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374466.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            complement(1131..1625)
                     /locus_tag="U2M88_002107"
     CDS             complement(1131..1625)
                     /locus_tag="U2M88_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374467.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            complement(1625..2074)
                     /locus_tag="U2M88_002108"
     CDS             complement(1625..2074)
                     /locus_tag="U2M88_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374468.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(2078..2695)
                     /locus_tag="U2M88_002109"
     CDS             complement(2078..2695)
                     /locus_tag="U2M88_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374469.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(2695..3603)
                     /locus_tag="U2M88_002110"
     CDS             complement(2695..3603)
                     /locus_tag="U2M88_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HEM8374470.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(3616..6375)
                     /locus_tag="U2M88_002111"
     CDS             complement(3616..6375)
                     /locus_tag="U2M88_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEM8374471.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            complement(6372..7076)
                     /locus_tag="U2M88_002112"
     CDS             complement(6372..7076)
                     /locus_tag="U2M88_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEM8374472.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(7088..9397)
                     /locus_tag="U2M88_002113"
     CDS             complement(7088..9397)
                     /locus_tag="U2M88_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEM8374473.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(9592..10056)
                     /locus_tag="U2M88_002114"
     CDS             complement(9592..10056)
                     /locus_tag="U2M88_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374474.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(10056..10682)
                     /locus_tag="U2M88_002115"
     CDS             complement(10056..10682)
                     /locus_tag="U2M88_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEM8374475.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(10689..11054)
                     /locus_tag="U2M88_002116"
     CDS             complement(10689..11054)
                     /locus_tag="U2M88_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEM8374476.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(11044..11382)
                     /locus_tag="U2M88_002117"
     CDS             complement(11044..11382)
                     /locus_tag="U2M88_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374477.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(11372..11698)
                     /locus_tag="U2M88_002118"
     CDS             complement(11372..11698)
                     /locus_tag="U2M88_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEM8374478.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(11676..11957)
                     /locus_tag="U2M88_002119"
     CDS             complement(11676..11957)
                     /locus_tag="U2M88_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEM8374479.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(11959..13323)
                     /locus_tag="U2M88_002120"
     CDS             complement(11959..13323)
                     /locus_tag="U2M88_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEM8374480.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(13336..13905)
                     /locus_tag="U2M88_002121"
     CDS             complement(13336..13905)
                     /locus_tag="U2M88_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEM8374481.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(13877..15064)
                     /locus_tag="U2M88_002122"
     CDS             complement(13877..15064)
                     /locus_tag="U2M88_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEM8374482.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(15128..16852)
                     /locus_tag="U2M88_002123"
     CDS             complement(15128..16852)
                     /locus_tag="U2M88_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007725426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEM8374483.1"
                     /translation="MEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIWF
                     DVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVTR
                     EAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILNN
                     PILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDEL
                     HKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSNF
                     VLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLDV
                     FVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDEDG
                     KAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDCM
                     NLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQFD
                     NPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDELD
                     DYLSSIYR"
BASE COUNT         4711 a         3680 c         2587 g         5874 t
ORIGIN      
        1 tgtttcctcc ttcttcgctt tcagccgaga acattttgta ggttcgattt gatacaccca
       61 acacactccc taaaaacgcg ccaaaaccag taatgatgac aacacagata tctgtgtact
      121 gccaattgag cgctttacca actaacccca cgaaagtagc tagtgcggga ataattacca
      181 gtgcgaacca ttttagtact tcgaacgttt tattattcat tttcttctct ccctaaacaa
      241 agttttgatt tgttgcgtgt gttccaccaa tttttctgta tgtgtatcta atctttcatc
      301 gtgtttcttt agttcttcat gaatcatcaa tcgatctgat ttgctcgatt ctaaatcttt
      361 agtcagcaaa tctaaattgt ggcttacttt tgaaagagtc tcagtaattt tcgagaaaga
      421 tgcagcaatc ggtctaatta ctaataaaat caaagaaacg atagcggtta ttgatcctgc
      481 tatcattccc cattccccta aattaatcat gtgacaactc ctttacctta aataaaaacg
      541 catcaattaa gatgcgctct tatctttatt aatgatttta tctgcttctt cgtctgtaat
      601 gcataatgga acgaaaacca ttacttgttc gttagtgaaa cagccccagt catacattag
      661 tttgatatct tcatatgaat acatgttact tcccaccttg taattgtttt ttgatttctt
      721 cgatttcctt ggtattttgt acagaagcaa gcatcgtttt tgaattcaac tgcgctaaac
      781 tttctgactt ttctttcaat aagacatttt cttgcttgat tgctacattg tttagcattg
      841 ctttagaatt tagctgtttc aattcatcat tttctgcctt cagcgtttcg tataatgcct
      901 taatattagc caattcatct acgctatcaa cagcattccc ttttttctct tcttcagtag
      961 ctaactcaac ccactgtttt ttatcaaaat caaatcttgg tctccagtta ggaactggtg
     1021 gtttaatctc tgtacagttt tcagggatat tttcttgatt gttcaaaatg atttgctcga
     1081 atccgtaagg cttgattgat ttgtaaacta ctttcattag atttcctccc ttaaattgaa
     1141 taagtgataa caaatgaata agctgatcca taacttgaat ttcttctcca tttaatggct
     1201 ccgtctgcgc caatagatag ttgagcactg ttcaaagtag aacggtctat tgacccaacc
     1261 agttgttcaa aactaattgg tgtccgatag ccttctggaa cagttaaaat aaccgaatca
     1321 ttcccgccac tactttttcc gtttaaagcc acaaaatata tagaaactgt ttttccttca
     1381 cgataaagct ttgctgtccc tgtattaccg ttagtgacaa ccagatttac cacttcatat
     1441 ctgttatcat cagtcgtcaa aacacgttta ttattgattg ttacagaacc tgaaaaattc
     1501 ttatgtcctg caatggcttg gtctccatct gtctgcacca atgcttcttt accatcaata
     1561 gcttctacat gcgtttttaa gtatttagca actccgtctt cttttaattg aacaatatca
     1621 gccattaaac cgtccccact ttctcaaatg taatattcgc taatccatca agtttcactt
     1681 tatcggttgc tgacattaaa cctgctgtcg tagttgtagc attacctgga tttttctgtg
     1741 ctccagctgc aattccatcc aacttagttt tatctgtgga cgacatcaac ccatttgctg
     1801 tagttgtagc tacagctgta gttgtggcat ttattccagg atcaccttta tctccctttg
     1861 gtaaaacaaa attaaatcta gctgcagatg atgttcctac attcgtaaca gaagcggttg
     1921 aaccactaga aacggttcct atggtaattg ttgctgcttg gccaggatcg cctttatctc
     1981 ccttcaccgt tgttggtttg ccttctatag cattccaatg agtttgtgga taaacctgta
     2041 caccgctttg ttttattttt acgatatctg tcattttcta tacctccccg attttttcaa
     2101 aagtaaaatt tggaattctt tcgtttgtgt aattttctgc ttgatttaca gcttcttgga
     2161 atttttgatc tacatatgac tgattaacac caccagtccc actaccacct gtagaactaa
     2221 ttgttccatc ttctgcaatt gatatatttg cacctgcttt taatattttt agagattcta
     2281 attttccctt taattcttca gagaaattga agtctgtttg cttagttgca gataaaacac
     2341 cttcttcagt aacttctaag agttccccaa cttttatacc tcctaattgt tcaggagtag
     2401 caatcggcaa aatatatgtt ccaccttctc catttacgat cttttgaaac atttcagcag
     2461 taagaatacc atcgcttgtc tcgcttgcat aaggtagttc agtaattgca ttctctaatc
     2521 ctaaatcagc tttcgttaaa atgactgcac catatttacc attaaccgaa agaacttttg
     2581 attgtcctga tatcattttt tctaagccta aaactgcaga aacatgtgta attggcataa
     2641 attgacgttt aacaccagat tcatcagtct ccatcattct ctttacttta accatcaaat
     2701 caccccaact ttctcaagag tgaaaacatt ctgttttgga tcatcaacag ttgcaataat
     2761 caaagctcct tcttcaattg gaaattctac tgtttcaatt ttttcgactt cgtgattatc
     2821 tgaaaagaga tcgtcttgaa gaatatcagt cacttcaatt tcaccgtatt caatcgtgaa
     2881 aagtgctgct ttcatttttt gatacaaata atttagatcg gataggaaac gttcagaaat
     2941 cgattcatgg cgtacacctt gaagatctac acgtgcatcc attaattcgg ctaacattac
     3001 tccacctgga tcgatagact tcaaaatatc cttgattgat tcaaaccatg acaaataatc
     3061 tgattcttgt ccttctctcc aatcttggaa actattctcc tgttcttctc tccagcgatc
     3121 aaattcttct ttcctttcat tcatccagtc cgtaaaatcg cctttatttt cattaataaa
     3181 atctgtcata tcagcaatta aatcttcaat tgattgccaa taagaaccca tttctccttc
     3241 tgtttttgaa acagcattga tgacaaaata ggagaagtct tgagttgttc caatcaagtc
     3301 ttctccttta aatataatga aattggctgt ttgtcgatgc aaacactgca tggaatattt
     3361 atcaaaaata tatttgattt tccctttttt agcatccaca attcttgttt ctaattggac
     3421 tggatatttt ccgccaacaa ctgattcaaa atatacctta cattctgata aatcatatgg
     3481 aagaccattt tcgacaattg tagcttccat aacttcagtg tttttattgc cttgtcgaac
     3541 ttgaatcatc cccacgtaat tatagggttc tgttgtactt aatatgacat tccactttgc
     3601 cattaaatca cctccctatt ttggtggtat gacaatggaa gaaattgcac ttgcactata
     3661 atactggcga tctaactttc cacaaatcat acctaactct gtgttttgtt cataagtttg
     3721 catacgacca ttagctaagc ctcggataac gccagtatgt ccataagttc cgtctgcaaa
     3781 ccatgaaccc acttgtccac ctcttgccca attaataatt gcaccaacta ctaattgatc
     3841 atatctaggg ttttgaatca ctttccaacc aacagcagac caatcatagg caataccaat
     3901 atcagatgct gcagaagtat ttcctatcac atgagttaaa ccatattttg ttccagcacc
     3961 catgccacaa ccacccagat atcctgaata ttctgcagac aagccataac actgaccatt
     4021 gccaattcgt tgtccaatca aagactctaa atgttttagt cctgcttctc cagtagctcc
     4081 tcctggtttt aaatctttaa atttgttata ccagttaact gcataatctt gacgttcagg
     4141 atgtgtcgct gccggacgtt catagtttcg ttcaaaagca tatgctgctt gtcttggatc
     4201 agtacaggcc ttaaatccat caaccgtcgt aggttgtact acgcccatcc attgtccatt
     4261 tgtaaatgtc caaataagca atcgaacttg tgcatctaag ctcgttattg gttctttaat
     4321 acctgcagca ttaaataagt tttgaacata aacttttcca tcccatgttg ctggaccaac
     4381 aagaggatat gaagaaccat cccactgaac taatccgtat gctggtccac ctatttgaac
     4441 agtatcagga tcaaaagttc ctcctgtttc ttgttgaatg ttccccaata ttccacatgc
     4501 agattgtttc gtaaatccgt tattacacaa aatatcgtat attctccaag ctctcttttc
     4561 tgcatctgtt tttaattcat caggataccc accaggtgtt tctcctccgc cagatggacc
     4621 gccattttgt cctggtatca cttctttacc tctaacagta agttttccct gaatatctaa
     4681 atcaccgaaa taatatgctt ttccgtttcc taataacaca aatccttttt ctgcagttgg
     4741 agaaattaaa atgtattttc catcgccatt tgtacgtatt accaaagaat tttcctcaag
     4801 aggtgtaggt gttacagcat ttggaaatgg atttcctgct gaatctgttg ttccgatcgt
     4861 tccaatggat tcttttctat tccagaattc catacctttt ttggttagct ccatgatttt
     4921 tttgttttca ttccaaattt gaagcgtgcc ttttacaagt tttaacacat caccataagc
     4981 attaaaggaa gtttcgaata cttctgcatt tattgttcct actttgatga agtcagcaac
     5041 aatttctcct tttgatgtga tagcaatacc gaacgggcca tttactccgt tgtctgaata
     5101 acctaagcca tttaaattcc aacgccacac tcgctttgca ttcgccacat ttggagtatc
     5161 catgataaga atctctgatg gagctttttc tggacgaaaa acgacatgcc caccagaatt
     5221 accagtgatc catgccgtcg cattcaacac attttgaact aacgtttctg ttcgattgtc
     5281 gattttcttt ttcaattctt gtgcttgagt atttactgcc gaagtgtaca gtgataaatc
     5341 attacctaaa acaatatcct tatatttccc aagcgttggg aaataagtgt attcgaccat
     5401 acgctctttt atctcgatat ctagctcttt cgccctaaca tgagcgacat caccaaagtg
     5461 taaagacgct agctcttgat acatgtcacc gtattcgagt gtgtgttcca acgccaccat
     5521 actgactgta tgagtagctt ttggctcatg gatacgatca ttgtcaaata aggttttgcc
     5581 ccatttgatc aattcatcaa ctgttttaca atctccgttt tcacgtttgg caatacgtcg
     5641 attctcgttg gttactccat caatttctaa ataaccatat tcgataggtt ctttgtcttc
     5701 atcataatcg ttatcaggaa caccgccaac caaatacaaa ctattgacaa ttgattcttc
     5761 atcaatttct tcttcgattg cttctaaatt gatgccaaaa tcaatccgaa aaccattatc
     5821 tgatccaatc tgttttacca gtttcaaatc atagttatcc atatcaagtt ccgcgctagt
     5881 cacgccagtc aaattttgat taccgttatt tgaaccaatg attgcttcaa ctggcgcgac
     5941 ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa agatagttaa atttttgatc
     6001 aaatgccaaa ctgtttttaa gattggtcat gatctgagac ccattgccgt tatccgtaaa
     6061 cgacttgaca ataaagttct tattggccat aaaaccaata tgccgagctg ttacagaaac
     6121 ggaggtcaaa ttctttttga cattataaat ttcaaaatat tgccatgatc catctggtac
     6181 cttcgctttt ataaaatttc ctttttttaa ataagagcga tactgtccgc ttcttgaata
     6241 gttaccataa aaacgatatt ggccattcag cacacgattg atttctggca aatcttccca
     6301 atcaactaac gacgctccgt tcacgctcaa atcatctggc atttttttat aagcataaat
     6361 aaattcttgc gtcacagata cacactccta ttccaaaacc gcacttcttt aaattttccg
     6421 gatattttta cctgattcca ttcaggctgc aatactggcc aatcaccacg cgtaaataaa
     6481 ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa ccacgattgt tgcagctaac
     6541 gtattaagta ttgttaggct tttatcaccg acagcgatcg ttatatctcc tccgttcgat
     6601 tctatttcca gatagggatg tgcaatttcg tcaccatgat caaaaatatc cataacacta
     6661 gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga gtggctgaca acgaaaagta
     6721 acatcgaatg tataaaagaa gccccactcg ttttcgaaag ggacttcttt atccatgctg
     6781 cagatagcct ctagatactt gtctggatca ttatgtgtga ttaattggct tttaccagtg
     6841 agccatcgtt tgacttctct cagtttcgaa tgtggaatag tgatcccttc tatttccaaa
     6901 tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc cgcttcggcc ctgaatcgtg
     6961 taagtttcgt atcgcttgtt cggcataata tctggaagct ctgcctcaat gatgcaattc
     7021 atatcaatta ctgcattccg atttttccaa ataaaatttg gttcatctgg attcataaat
     7081 tgtcgactca aataggaaca cctcccaaat cacgaatagc ctgtttgttt gctttagcaa
     7141 atttacgatt catacgatct aactcagatg gattgtttgc atcgactttg ccaatgtgta
     7201 tatgctgttc aatgctacct ccagaaactc ttccactgat ccctttgcgt ttttcttcgt
     7261 ctgataatgg agtaactgtg gttttaccat tcttggcagt cagtaactca ggtccagctt
     7321 caccaacaat cgcttgtcca ttgattagat gaccgccttg tgctaaataa ggcagttttg
     7381 aaatagaaaa tgattttcca cctacaccag gaacccattt tggtatcttt atattattca
     7441 atccaccaat aaatccattg atcaatccaa tcatggcatt aataggagct ttccctacag
     7501 caacaatacc atcgaaaata cctccaaaaa tatcaactag accttgccat gcttttgacc
     7561 aattccctgt aaatactcca gtgataaaat ctagaaaacc actgaaaatt cgttttccag
     7621 cattataaaa attattgaaa tttgctataa caccatcgaa aaatccacca aaataaccac
     7681 ttaaaaaatt aaatatttca acagctacat ctgaaacacc tttgaagaaa gaattcactc
     7741 catcatgaaa ccatttaacg ttattatatg cccaaattag tccagtaact aatgcgccaa
     7801 tagctataac tgctaatgca aaccagcctc ccgacattcc aaataaacca gcaagtcctt
     7861 gccaaactcc tataaaactt ttaacttccc caacgatttt tgtaacagat cccataagtg
     7921 aaccaagaac gattagtact ggtccaactg ctgccgctat accagcaatt gttatgatcc
     7981 aattctttgt atcttgatct agagaccccc accatttaga gaattcctgt aataaggaag
     8041 ttgctttttc aaaaaaaggc aaaaggctta cttggactgc ttcacctacg tcagccattg
     8101 ctaattttgc attgttcata gcttgatcgg cttgatcaat tgggtcgaga gtagcatcga
     8161 atgtgtctcc tacagctccc ccactttctc cagcagtttt tgctaaatct tccaggttca
     8221 aagtacctct acgaattgca tcggccattc taggaccgcc tttagtcccg aatacttctg
     8281 ctgctgcatt gatggcttct gtttcagaac ctgcattttt taccttgtct tgtagttctt
     8341 ctagtccttg acttaaagat tttccgtctt tcgcataagc cactgttgct ttagataaac
     8401 tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa ttgtccaagt aaagtgaccc
     8461 cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc gccatcaatt gctttttgca
     8521 tcaagtcgtc tacagattga ccagtattct gcgatgtttt cgttgttaca tcaaggacag
     8581 aattcaaatc atcatattct agtccataag catcaatagc ttgtcgagca gatattgctg
     8641 attgcgaaac atctgtatca ttgatttcag cgtactgtag gagataattg gttgaatctt
     8701 ccagtttttt atccatgaat ccaaattgtg tatttacctc accaatagct tccccaaccg
     8761 tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa agactgtgaa agtctatcag
     8821 cttgatcacc tgttgctccg gtttttgtaa tgatggtatc aagtgcctcg tcaacttcac
     8881 caaatgctgc aagtcctgct gctcctgccg ctacaatagg cgcggttact ccaacagaca
     8941 ttttttcacc aacgcctttt actttctcac ctgtttcttc gattttctgg agtttttttg
     9001 ctgtatcaac agaaacgtca ccttgttctt taagtgcatc gttcgtctgt tctaatgctg
     9061 tacgaagttt attttctccg gtttcagaat tgagtaattg tttataaagt ttttctgatt
     9121 cagcagaata ttcaccggtt tctttgacag atttttcata ttcctctctc agcatttgag
     9181 atcgttgttc agccaatcct aattgttttt ctaatttttg tttagccgct cgcaattttt
     9241 ctgtttgagt tgcatccttg tccatcgcag acacttggtt tttgtactcg gcagcggcta
     9301 agttcatttc tttgttgatt tctttgattg ttttcgaata actgacttcg ccattcgttt
     9361 taaaattaag aacgacatca gattctttac cagccattta tcttagcgct cctttctacc
     9421 accaaggcga tttatccata gttacagatt gaggtggttc aaaatcggtg ttttgttgta
     9481 accactgtaa ataagatttg agccacaagt taggtgtaga ctttaaaaag aaactctcac
     9541 tccaattcaa tagagtgaga gcaacgtaaa tataaaaact ccaaggagtt cctatctctt
     9601 ccgattcttt ttgtttttct ttctttttgc ttgcgctttt tgaaagtctt gtggcttctt
     9661 ggattttttt aggtcttcca cctgaaatgt ctggctagca aaaatttcca tacaggtact
     9721 ataagcagac aacacctctc cactcattcc caaaaatttg aaaatagttt ctggatcttc
     9781 ctctaaacca ccagtacgca gcatggcata aatcaaggca cgcatgattt ttagatcact
     9841 aggcgataag ttagcagaag aaatttgtcc ttctttttta gatagcatgg cgttcatatc
     9901 agattcaaat tgcgaataat ctccaccata cacatctgct ataaattcca tcgtttgcat
     9961 agtaaatgaa atagggaatt ctgcgccttg aataattaca acagcagaat tttttaagtc
    10021 ttcaacgtga attccataat cagataaccg agccattaac ccgcacctcc aactttagcc
    10081 aatgttttcc actgttcttc atcgtataca ggttgagcaa tgaatttatt aaataattct
    10141 agagaagctc cgtctctatt tgaatcaaaa cttgcataca tcacattatt gtatttcagt
    10201 ccgatggaaa ccagatttgc agttacatcg tcaatttttg tttcttcctc tgcagtggca
    10261 tactcttcgt caatcacatt ggatagttgt gtattaggat accaaactgc ttttttaccg
    10321 ccattttcaa tatttccaat aaatccaaag gcgaagtacg gaaattctcg tgcggtattt
    10381 ttgctaaacg ttactccgct tttagcaact aatcctttga tttcatccat cacttctatc
    10441 ggaataccca cgtgatccaa cgcaagttca tgttttgttt ctcgcgacac acgacgaaac
    10501 attttgctgg atgcccattt ttctaatgct gttccattcc ctttgacacc gatttttgtg
    10561 gcaattggca atcgtaaaac ttcactataa gtcggtgcag ttccaacaga atctggcgtt
    10621 gccatcatcg caattaaaat gtcgtccaat ccttcaaaat aaaaagtatc ttgttttccc
    10681 aattaaaatc acccttccca taaatcaagt atttttctag tcataatttc ttcaattttt
    10741 tctttgtttt gttcatacgt accacttgca aaatgttggg cacgttgatt tgttgtgcca
    10801 ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt gaactttcac ttgatcgttt
    10861 tcaactacta cttttatttg atcgcgcata tgtttctttt ttaagagcga cttaggaata
    10921 ttaggaagca actgctttag ataaaagtta gctgcttctt ctaaagattc caacgataat
    10981 tttttcggat caacttttga aaggtttcct aaataatccg atatttcaga aaaaccattc
    11041 ttattacttg gcattttcca cacacctcac atatgtgtaa tagttggtta cggtatcgtc
    11101 gttctcatca ccttgtatac ctgtaaaatc tgaatatgga atgccagcat tttgcagcgc
    11161 ttgttcaata acaactaaat cctgttctgt tccaagtgta aaaaaagaga cttggtaata
    11221 aggtaatttt ttatagactt taccggatgc catttttttg ctgttactca catttgagta
    11281 cacaatgtat ggatacatcg tccctaatct ggctttgtct ctgaacactg ctaactttgt
    11341 tgatttcagc gctgttttca attcatcaaa gctaatcgac ataagctaaa ctcaactcca
    11401 tttctcttgc atcgggattc gtataaatgc gagtaatgtt atacgttaca gaatcaattt
    11461 tgagcgcact taatttctct gtgatggatt tatcccatct gactttaatt cgtctgacaa
    11521 cgtctgtctt ggcttgctgt gataaatatt tttcttgaga agtcacaccg agttcttcat
    11581 agaatattag acgcttaaat tcgtaaattg tagttggacg atcgtttccg tctgttcctg
    11641 ttttgatgtc tagcaattcg gctttccatc tgagattatt ggtctgtctc ttcggcattt
    11701 tgaatcgctc cttgcacgat aaatggcgtc atggcattca tagctttgtc gagttcatcc
    11761 tctgaaactc tatattcata ggcaatgccg gcaaccatca aaataagata ttcttgttgg
    11821 ccaccagttg ctgttttgac ataatctttt gccatattta aataaaaaga gagcaaagaa
    11881 tcatccatgc cctcttcaaa atgaatatgt gatttgaatt tttcctctaa agacaattct
    11941 ttagtttgct cttccatctt aaccaccaac tggttttgta atttcgtagc gatatactgc
    12001 cggttcaaat ggagaataaa ccaattgacc atctagcaag ttgtaaattt ggaatccaat
    12061 ttgattttta ccagagaatt tttcaacaag tttttgaatt tccaaggcac caataacttc
    12121 ttgaatttta aatgcagaaa aatcgccaaa atataaaact ggcgtgtctg gttcaccctt
    12181 tttatctgct gcatctgtcc aatccacagg atagccaact aattggtaac caattccacc
    12241 ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca tctgttttca ttttttcaat
    12301 ggcagtcaaa gcagctcgat taataataaa gcgtcccttt ttcatcactt ctgtcactgg
    12361 tgtattttta aattcgatta atgcatcata taatttttgc ccagcacctg aagcagttag
    12421 atctaaaggt tttttaaatg ctacagcctt tttggctaat gcaccaggat tttcatttcc
    12481 agcgtcatca ccattgaaca tataattgat ttctttacgc acataagctt ttttcaattc
    12541 ttccacaaca atatcttcaa ctggaacacc agacattttt aataattttt tagttactgt
    12601 tgccaaagca tcgaattcgg caggattaag caaaatttca tcaaactgaa tagctgtttc
    12661 agcaatatca gttgaacgct ctttcttgtt tacattcgca tctgctttct tcacaagaat
    12721 tggatatttg acatctcctg atgttcgcac cactgttccg tatttacgaa gtaaattttc
    12781 ttcttgagca taagtaataa cttcagatgc aattacttct gggacagtaa ctgaaccgtt
    12841 gccagcttca atccctaaag ctcgagcttc tgcttcagaa atatttccag ctacaaaatt
    12901 agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg gtcatttttg cacgtgcctc
    12961 caatccgttc ttaattgatc caagtaatcc atctcgttgc tgttgagtaa tcattccaga
    13021 acgattttct gaactatctt cactgtctga atcttcttct tgatcgttgt cttcaccaga
    13081 acggctttcg tctgaatccg tactattcga ttgatcatct gtattgtcgt ctgtttcatc
    13141 agttccagaa tctgcgccta attcgtcttt aattccgttc aattcatcaa taacactgtc
    13201 aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat tctcctgatt caactttttc
    13261 acgtaattca gtcaatcgtt gctcacgacg tgccttcatt tttttcaata attctttatc
    13321 catgtatttt tcctcctacg cttctagcgc ttgattaatt tttttgatta atttttttct
    13381 ttcttcgacg tttttttcta attcatcacg acttcttaat gcagcttctg tatcttcgta
    13441 tgctggcaaa ggcacaatag aaacctcgta taattccact tcatggattg ttcgaagcat
    13501 tggttcagaa ttgtagtccc acgtttcttc agtcggcaca aaaccaaaac tacattggtt
    13561 gatgtctcct cgttccatag atttaactaa gtcccttgct accgttgtgt ccggtaaatc
    13621 aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga agggttccgc ttttagtacg
    13681 tcctagcaca ttggaccaat cgtgattgaa caaacaacga acatccgaat tactaattgc
    13741 ctttgagaat gcaccaggaa caatcacttc gcttaagtca tcccataaaa gtgtttgact
    13801 gttgaataca gcagcgtagc cgctaatggt cctcgtatga gtttcttcat cggaacgtgt
    13861 tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc ttcatttcca tcacctcctt
    13921 tcaagtcttg atcattcgtt ggtaaggaat catctgttgc atttttctgg ccaatcctag
    13981 ataagtcatt tgaaatatag atagcttgtg tttctggagt attctgttta ggaaaaccaa
    14041 gcatttctgc cacgttatct ggacttgtaa tcccggtacg aacgatgttg tagccaatat
    14101 ttgtttttgt tgagtaagga acaaaatcca aaatattaat tttccattcc actctatagc
    14161 cagaattagg cataaaaaaa agagcggtgt aatgttcgct cttgtttttc aatattggtt
    14221 tgattgcttt gttgtgcaga tacatcatcg ctttttcaat atctgtcttc atcagcgatt
    14281 gatacgtatc aacattgatt cctagaaatt ttcctaagtc ttttttgtaa acacctaaat
    14341 aattcaaaat tgccgcgtca tcaacaggac tttttaatgt ctcgatggaa tatccttttc
    14401 ccaaaggaat catcttaaca gaatgatcac tctcattttg cgttccttcc agttgatcca
    14461 atatagcttt gacaattttt gtttgggcgc tattatttgg attgatgtgg gcgtctagtt
    14521 taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc cgtcaaaact ttttcagcgc
    14581 tcagaacgcc ttctagagtg tttcttgcaa gatcaattat tccagcacct tttaatgaat
    14641 cagttccgat gttcttaatg tgtcgaatca tttgaccagg tattttttga ccattcattt
    14701 caaattcttc ttgaagtcgt tcattgattt tagttgttac accgtacgcc aaatgaagct
    14761 gatccctatc cgttaatggg aatgtctcac cattgatcaa taaagtattt gtttccaatt
    14821 tggtaaattc gaatcctgtc aaataatcat tgggactctt caaaatattt agtaagtgat
    14881 gatctttgac ttcgttgccg tcgggaccaa taacaattgg tgtggccaat gccacctgat
    14941 tagatatgtc ttgaactaat tcataaacat cagaagattc catgatagat gaatccgtca
    15001 catatctttg accataacgt gtgtagtgtc caaaaatatc ctcgatatat ccacgttttt
    15061 ccataaaaga atagactgca ttcgataacc gatcacgtaa tttcaatttt tttcaccgcc
    15121 tttctattta tctatagatg gaacttaggt aatcatctaa ctcatctgaa tcaatatctg
    15181 tcatttgatt catcgtttcc ttatgaccac acaaaaacgc cacgaaccca tcaatctttt
    15241 tctttgattg acgtttactt ggcgcttttt gtccattgat gttagtaatt gctacaacgt
    15301 tcaaagtgca ataaaggaat aatggattat caaattgaat tcgtttctca taaaacaacc
    15361 gttcgacatc gtcaaaagga gcattcaata ctttaggata ttgagcaact tcaacgcatt
    15421 ccaatcctaa gttttctaat ttctcaacaa gtttgtcact catcgctgga tcataattca
    15481 cttgctgaat atcatataaa ttcatgcagt cctctatgta ctgcaaaatc tgatcttgat
    15541 caatcatttt tccgtcgcaa aattcaacga aaccttgttc agccaaatca ctgtaaggaa
    15601 cattatcttc tttttctcga aactctaaat cttcattcgg aataaaataa agttgcttta
    15661 ctttaagaac cgctttacca tcttcatccc atgttgggaa gtttaaagat acacaggtca
    15721 aatctcgtgt acgtgataaa tctaaaccga tataacatgg ttcgccactt aaatcaccaa
    15781 gttcttgtgt ggtaactaaa catggctcta cttgatcctg ttcaaagaaa ttatccgcgc
    15841 cgttcacaaa tacatccaag tgtttcgtta gaaattcagc tttggaatga gctgaccgtt
    15901 gcgctgtttt aaatgccgat tctaaagcag ataaatcgac tgatattccc cagttaggat
    15961 tgcacatttc ccagactttt ctatccgtcc aatcataatt tttatttggc tcatagatta
    16021 gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc ttttgcttcg cgatatacac
    16081 gcattccaac agacgacgaa cctttcccag cagttgagat attaaacatc aatggctgtg
    16141 gtaaagatat ctgagcagac ttaaaattgt catactgttc cattttctcc tgtttatgca
    16201 attcatcgtt caatacaaaa tatggattgg atccctcgat attatcaata ttcttggttt
    16261 gaacaataaa tttatttgag taagccatat cttcatgaat atagtcataa gtaatactag
    16321 aaacagttcc ctttggcccc ctaaatattt tagttccttc aagtaggatt ggattgttta
    16381 agattgtaac tgcaaaaggt ttggcagcat attgcgcttg agcaaaatca gatgcgcaag
    16441 catagcaatc aactgataac gccccttctc cgtacattgc atatcctaat gaaccgacag
    16501 caatcaatgt tttaccattc ttttttggaa tctggacata agcctcacga gtaacgcgta
    16561 caatttggcc tttttcattt tctttaaccc aaccataaat ccaagaataa ataaattttt
    16621 cccagggttc taaaagaaac ggttttccca ccatttctcc ttttgtgtga acaataaatg
    16681 attctaccca atccatcatt tcattcgcgc gatctacatc aaaccaaata tcttttcttt
    16741 ttttccactg ataccagcga tctattgcta aacgaacggt cttaggatac ttcttaggtt
    16801 tctttcttac ttcttttgca aataaatcag cataatttac accaggttca at
//
LOCUS       DASHRQ010000059        16764 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.063, whole genome shotgun sequence.
ACCESSION   DASHRQ010000059 DASHRQ010000000
VERSION     DASHRQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.063"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(99..1229)
                     /locus_tag="U2M88_002124"
     CDS             complement(99..1229)
                     /locus_tag="U2M88_002124"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEM8374484.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="U2M88_002125"
     CDS             complement(1878..3080)
                     /locus_tag="U2M88_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEM8374485.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="U2M88_002126"
     CDS             complement(3140..3343)
                     /locus_tag="U2M88_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEM8374486.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="U2M88_002127"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="U2M88_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="U2M88_002128"
     CDS             4880..5692
                     /locus_tag="U2M88_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8374487.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6715..6867)
                     /locus_tag="U2M88_002129"
     CDS             complement(6715..6867)
                     /locus_tag="U2M88_002129"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEM8374488.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7058..7936)
                     /locus_tag="U2M88_002130"
     CDS             complement(7058..7936)
                     /locus_tag="U2M88_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM8374489.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7975..9093)
                     /locus_tag="U2M88_002131"
     CDS             complement(7975..9093)
                     /locus_tag="U2M88_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEM8374490.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9090..11189)
                     /locus_tag="U2M88_002132"
     CDS             complement(9090..11189)
                     /locus_tag="U2M88_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEM8374491.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11240..12061)
                     /locus_tag="U2M88_002133"
     CDS             complement(11240..12061)
                     /locus_tag="U2M88_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEM8374492.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12058..12867)
                     /locus_tag="U2M88_002134"
     CDS             complement(12058..12867)
                     /locus_tag="U2M88_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM8374493.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12888..13382)
                     /locus_tag="U2M88_002135"
     CDS             complement(12888..13382)
                     /locus_tag="U2M88_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8374494.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13394..13882)
                     /locus_tag="U2M88_002136"
     CDS             complement(13394..13882)
                     /locus_tag="U2M88_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM8374495.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13959..16655)
                     /locus_tag="U2M88_002137"
     CDS             complement(13959..16655)
                     /locus_tag="U2M88_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEM8374496.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         5096 a         3610 c         2696 g         5362 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt ttcttgctat ctttttataa
     6661 tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa tctatcacca
     6721 aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa tgaacataca
     6781 ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat tactatttaa
     6841 gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag aaaaaaggaa
     6901 acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac ttagagcctt
     6961 tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt actcggttaa
     7021 atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta tttcttaccg
     7081 cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat tcgggcgtaa
     7141 taatgtagct atcaattggt ggtatttcaa catatccatt tataagctca acaaatttat
     7201 cctgaacttt tttcttcatg tgttcttgtt tcattacacc accgccaaaa ataattctat
     7261 ctggcgaaaa catcagtgtt gtattgtatg cacattgggc aaggtaaaag gcttcaatct
     7321 gccaaaacga atcgtcagcc aataaattct ggccttttac acccaaccgt ttttcaatcg
     7381 ctggtcctgc tgccatcccc tctaaacaat cttggtggaa gggacagttt cctgaaaaat
     7441 tgtcctttgg atgtcgcttg atcaacatat gccccatttc cggatgactg aacccttcca
     7501 cgaaagtccc attgaacaag gcaccacctc cgatacctgt tcccacagtg aagtatgcaa
     7561 cacctgctaa ccctttgcca gaaccagcaa catattcacc atagcaagcc gcattgacat
     7621 ctgttgtcca ttcaatcgga atattgaaat attgctttag cgttcctaca aaattgaaat
     7681 tttgccaagc aagttttggt gttgatgtga tatgaccgaa actcgttgat ttgcattgaa
     7741 tatcaattgg accaaagcag ccgaccccga tgcctgccaa ttgttcctta aaaggtttaa
     7801 aaaaatcaat gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa ctgactcttt
     7861 ccaaaatcgt taattcctca gtaccgactg cacagacaaa tttagtccct ccagcttcaa
     7921 ttgccccata tatcatcata ggttcctccc ttcgaatatg tgaaaaacac ttcattaacg
     7981 cggcaagcgt tctcgatcag tgaaatgacg tttagtattt cctgaagctt ctgttgaata
     8041 ccagtaagcg acactagcaa catcgtcttg ccgctcaaat aagcgaacat catcgttgcc
     8101 gatttgttgt aaagtgaccg ataaatcttc ggtaaaacag atggggtctt gaagatgcca
     8161 acgataaaga ccatgtcgtg gcattgcgtc gtcaccaaag gcatgaacgg cattcaattt
     8221 tcccgtttca aaacgatctc ttgtggcatc tctatttgat tggtaaggat accctaaaaa
     8281 ggctgttgaa tagtttttcg catgtggacg tccgaactca tcgtactcat gaaaggccca
     8341 cgccccgcca aaataatctt ctgaacctgt tgaggttacg gtcggatact cactatcccc
     8401 atcaatgtag aatttaaact cgccttcgcc ccaccaataa cgttctaggc aggtaagcgc
     8461 aaggtaagta cccacgtaat agccttgacc ctttaatcga tcaattaact tataatcctc
     8521 ctgtaatttg gtaatgcgtt cccgatgcca atttgcgtga aaatataggc tattttcttc
     8581 caattcgtcc accaaagcgt aatttatcgt ataaaaaaat gaggacatat ccttcggatg
     8641 taaattctcg atcgtaattt tagcggatgt ttggaaaggc atttcgaaat aggaattaaa
     8701 gccccctgta ggattaacca ctatcggcaa cgagttcacc tcgcaacgct ctccaaaccc
     8761 attacaaaag aaatccccta aaggactttc aacagcaggg ctatccgacc catcccaata
     8821 aattcttaag attaaatcac gtaaaacaaa actgccaaat tctgttcgat cacgaatcgt
     8881 catccacata tgacgaatta ttcccgttcc agtaatatcc gcaatcacag tttcttcacc
     8941 agaagtcagc ggaattgcgc cacgaccttt gcggcttggt cctagctcgc ttggcaacat
     9001 cgcagcttta cctttctcgc ctgttggatt ttcgggagtg attgctcgac tctggatttt
     9061 tttaaattgg gtaatatttt ttagtatact catctttttc tcctttgaat gtgaatcgtt
     9121 tttccctttt ctacgtgaag aatttttttc gtatcactta ttttcaatgg cttttctgac
     9181 aaatttgaaa tactcaactc ggattgcgta cattcaatcg aaatgaaggc ttgaccgaac
     9241 ttcaaattct tccaagaaaa cgttaaatta tcaacaggat caatttgcaa tactcctttt
     9301 ttgaatttca atccgaaata ttgactgatc attaggctga caaaaagacc cgatgcccag
     9361 ccgcatttgc cgcagccaaa gtctcgcacg acttctcctt gttgagcctc taagcgatac
     9421 ggccaccacc accacgaacc gtctaaatct gccaaacaaa ttagttcttc taatcgggtt
     9481 acgaaagctt gagggttgtg cagctcgctc attaaaacag tgataaagcc aggaaaagtg
     9541 gcgccggatt ctaatcccca agtgatgcct ttgatttcct cgctataggt tggattgaaa
     9601 gaactagcag aaaaggccat ggtgttctga tataaatgat ctttattatc taaaaattga
     9661 taaaaaggca tcaacgtagt gtctgattct tctccatcat gcatcaataa attaattttt
     9721 ccatccacaa tcacatcact taaaaagatt aacccttgct caagaatcgg tttttgatag
     9781 tgcttcactg aataggttgt tttctcctca tcaccgattc cctccaaaaa ttgctcacca
     9841 aaaggaccat cgatagtcat ttgtttacaa attgattcct ttagtttaca ggcaatgttt
     9901 ttgtatcgct tgctaagact cagttgttct agggcttcaa aaatttctgc aagtccaata
     9961 caggccttcc aaagacaaag attgctgccg gtatgatact ttccaagtgc aaaggcgtct
    10021 gataaccaaa cagatggaaa aagcattggt tcccctgacc gacgttgagc taatatgtcc
    10081 tcaataattc ttgaagcatt cagtagaacc tgcggacacc gctcaaagaa ttttgtatcc
    10141 ccagtatgtt caaaataaag actagccagt aacgcactgg ataacgaatt actcagagaa
    10201 tgattgatac ctcctgtaaa ttttgaacca ggaaacttaa cgccatattg atcaaaccat
    10261 agaaccgttt tttgacataa ctccggttca aacatggtga atggcaatga tgcgtaatac
    10321 atatccttat tccaaattcg attagtagca ggatagctgc cccaattgct gccgacgacc
    10381 tgtccctgtc gattcatacc gaaactagaa aagctttgat aaagtgcacg acgatagaga
    10441 tggacaactc tatcgtttgg cattgacagc tgcccaaata gctgagcaaa atagttcaat
    10501 gtttccttca gccatttttc gatatcagat tcctgaaaag tcattacttc ttcaaaagca
    10561 tttgggtcca caaaagcaat ggcgaagctt gccacttcct gttgtgcgat cgtgtcctta
    10621 tttccttttt gagaaatcaa cacattttgc tgatccgaat acttctcttg atacgaaggc
    10681 acatctttca ctgaaacttg aaggctttcg tgtgtttcat tcacaatgta aacctgttga
    10741 acaagcatac ttaaacgctt attagccagt atgggagcaa aggatactac aatgacctta
    10801 aacgtcgaat aatgatgaat atatctaggc aaacagtctt gaatcacatc gacttcaata
    10861 cgatgatccg attctgacaa atggtagaca atcccgttta cttcaacctt taaggataac
    10921 gaattggttt gagagagctg tttattgacc caaacacctg gtttttcctc agttagtaaa
    10981 ttttctcgat aaaaggtcaa cgtttgaatc gtcccgcaaa catcaaggtt tgctaatgtc
    11041 ttttgattac ttacatcgaa ggtcaatccc tttacatcag gaatattccg atagtagctg
    11101 tattcgtttg aagtagtcac atttccgtcg tttaactcag cattaaaaaa tgggtgtttg
    11161 ggtgaataaa ttacttcatt aatagtcatc ttttccctcc ttgctaaaaa aactgcagcc
    11221 tagtagctgc agcttcctac tatgcgattc caagtaatga caataaaatt cccaagacga
    11281 ccattccaaa aataattttg atgacgccga cttttttatc tagtaatttg aaacagctca
    11341 atgttaccaa taacggtaca attcctttaa aaatactatc gaggatttct tggacattca
    11401 ttagttcttc gcccttcatt gaaaagctca aaacggtatt gaatttgacc atactgatcg
    11461 tcattgcacc taccatgatc agacccaaga tactagcagc ttttgtcaga atttcaatta
    11521 acccgttcgc atacagcttt tcaatatatt tagagcctaa tgaatagcct aaaacgccgc
    11581 catagtatcg catcaagtaa ttaggtatat tgaacataac taaaaagata attggtgcca
    11641 aaatatttcc ggcattggct aagttgatcg ctagtcccgc ggtaacgaca cgccaaactc
    11701 cccagaacaa tgaatcgcca attccagcca atggtcccat caaactcgtt ttgatcgcgt
    11761 tgatactttc cacgtcaaaa ccagcttttt cagaattttc cttctccatc gatgccgcaa
    11821 tccccatgac aaaggacgcg caaggaatgg tcgtcgaaaa atactgtgta tgacgttcca
    11881 gcgccgcagc tcgatcttct tcacttttgt agaaatgatt aatgaagggc atcatagaat
    11941 aggcgaaacc accgccgccc atcgtcaccg gattcaccga agcatacata gtcatcgagc
    12001 gccaaaacat cttccgaacc aattttttct catctttaga aattccatca gctaatttca
    12061 ttgaaagaaa tcctcctctt cttggtcaag cgttgcattt acagcagcat cgctttgaac
    12121 tgcatgaaca gatatatttt ttagttgtag atctctttgg gctataacaa ttgcaataat
    12181 cgttccaact gcggcaattg caatcaatgg taactcgaga tacgccataa caacggcccc
    12241 aaagaaaaac cacacagcaa tatttttact ccaaagcatc cgtaacagca tcgccatccc
    12301 aactgccggc agcagatttc cagcgactgt taatccgttc atgatgacct ctggaatatt
    12361 atccaagacg cttttgacaa catctgctcc aaataaaacg gcaaagaatg gaataagagc
    12421 ataaagcgca tagtggacaa accacataaa gaagtgcaga ttgatgattc ctttttgatc
    12481 accagatttt gcaaatttga aaaatttagc agccatcggt ccacatacaa aagaaaaaat
    12541 catattcatt aactgtaatc ctaatactgc tagtggaaag acaatgggaa taatcgcatc
    12601 cacgtttcct cccattttga ttgcgaacgc tgtagccagc ccagcagcca aagaaggctc
    12661 cgccgtacta gagattccga tattgacgac acccataaag atggcttcta gtgacgcccc
    12721 gacaatcaac cccgtctgca aatcccccat caacgctcct gctaacggag caacaacaat
    12781 cggacgactc agcatggaca tcccgaacat ttcatgtcca gcaactccaa taaaaacggc
    12841 caaagcgact acaaatgcat catacataat ttttctcctt cctttttcta aaaatcactc
    12901 aaaatagaga tagctgaacg tttttccatc gccggtgtag gctgaaaaac ggtatctgga
    12961 taaagttcaa ctaattcttt taagtcttga atttctgacg tcgttaacat cacttccttt
    13021 gataaggttt tcttatcttc cgatggcggt gtcattttcc cagcattccc cacgttcatt
    13081 aattcgattt ccgttgggaa agctttcgca acttttaccg catctttgac attagggact
    13141 aaaaccatca agcgcatgtc cttcgcccgc ggatctttta acaaatgaat cgcgtcatca
    13201 actgttttaa taatgacttt gacattcgat ggtgcagcca tcttcaatgt catcttttga
    13261 atatcgttgg tagccgcctc atcgcttgct actactaacc cggttaaatt tagactcttt
    13321 gtccaagtca tcgctacttg tccatgaatt aatcgttcat caattcgcac tgctttaatc
    13381 atttttcttc ctcctaaaaa aaatcttcct cttcatttac tacatgtacc tcatcaagaa
    13441 tggtcatctt taattcctgt tggcatttat ttaccagttc ctgaatatcc tcctttgtta
    13501 attccttggt tgccaatact atttcgagca gaattggcag attaaagcca gacaaaatag
    13561 ttacagcaat ctctttctcc aaaaaggttt tggtaatctt ctgattgaca cttccgccaa
    13621 ataaatcggt aaaaacaaaa atctgatcct gctcgttcac ctggtttaca aatacattca
    13681 actcctgttc aaaatcacac tcatccacat aagcattaat caccgtaatc tcttgaacct
    13741 tccccgtcag taattgaata ctgctaagaa agcctgaagc taagtgaccg tgagtcgcta
    13801 ttagaatttt cttcatatgg ttaattcctt tctttctgtt cacttttata taagcaattc
    13861 ctgtgccaac ttttaaaaac attggtttaa tcacgttaat tcacattaaa aaaagtgtct
    13921 aacctaaaaa aaacagatta gacacttttt tagacacttt atttttgaat atacggtcct
    13981 aataattcat agagcagcga aagctcataa gcattgactt taaaattata tttcatctcc
    14041 attgattgaa aaatacttct agaaacataa taaaattcat cttcatctgg tttcaaatca
    14101 taattcggtt cttcagattt ttctgtccga gccaagaata aacgttccat catcaatgcc
    14161 gtatgcatat agagatttag ttttacacgg ccatttaaag tgaaatgata gtaattttca
    14221 tatttagaaa tgactgtttc cacttcactc atcacgatcg ttgggttcag aaagttcaaa
    14281 cggtccgcaa ttccttccaa cgaaaagaat tttactagct cctgaatcaa gcggttaaat
    14341 tctgccggct tcatttcagg actcaaaata tgctccaaat tggctgctcc atcaccgtct
    14401 aagatgtcat aaacattaat attaggaatt gaaaaggaac gtggaagatc cgaagtcgtg
    14461 atgaccaatt ttgtcttgtc aaaataagtt tcatcatttt ggtcgattaa ttcacgtagt
    14521 tctctgtagt ccttcgtgaa aacttcgatg gattcatccg ataaatgatg acgaaaaaca
    14581 tctttcaatt tgtccgaaat ccccaaaccc gacatacagg aaatgataat gttgctactt
    14641 tgagaaaagc cttcaaagta ttgaacattg attgaatact tgtctgctgc atcctccgca
    14701 atctgtttaa atggcgcatt catcgtcatc ttcaagccaa tatcaagtgc aatggaagtc
    14761 gtcaaattat tcagaagaag taactcccct tttaaatcgt tcttgatctg tgtgtaaatt
    14821 tgatttaaag agcccatatc tacgatcagc actagactat ctgtattcac ttgccgtttc
    14881 acgaaattcc ttgtcgattc gacaatctct gacacattac tctctactgg catatccaac
    14941 gcttcaaaaa cataattgcc gcataattga ttcactactg cttgaatgct gctagccgta
    15001 ctatctccgt gagcaagcaa caggcccttc agctcgatgg attcatctac ataatcagat
    15061 aaagtcacga caataagcag tcttagaaag ttctcgtcct tttctgagag cggtggtaaa
    15121 tgctccaaga actgattaca taagtaagtc gtccgaggta gatttgcagc taatgtacga
    15181 tttatcccgg ttaagtctgt cttatagctt ggggaaatga cataggatct gctaataatt
    15241 tctgcaacag gtccactcgt cgtcaatccg tacgtttttc ctaaaacgga tacccagacc
    15301 tttctaaaca tctgaatcat caattttcta ctcattgata aaaaagaatc cttatcaatg
    15361 tgatgcacca aatgctgtac ttcattcttt atcgaagaaa aattttcatt gaaattacct
    15421 tcatttatca gctgagtaat tttttccaaa atcgaatcat aatcaaacgg tttgattcga
    15481 tccgttttta tcttttgatt tttctttatc aataaactag caatcggcaa gctttctact
    15541 tcggattcca gcatatttaa ctgaatcaag atctcatttt tcccaatcgt taatggttct
    15601 tcttgtgttc gactatacac atccgcacaa ctgattttaa tgatattctt cagcttacca
    15661 atattcccac taaagggatg agataacatt aaagacaagg cttctcttga aacaataata
    15721 tctttttgta aaacatccgc ttcattttga aaaaataatt gaatcagctc taatcgttct
    15781 cgaatggggc gatcttcaaa agttggcaac ataacactaa tttgaatacg ccgatcaaag
    15841 gtgtctaata aataattttc acctttttcc gtcgtggcga aaataaaacg aaccttagca
    15901 gaatgccaat ttacattttc accgactgga cgatagtttc cagtatcaat aaatagaaag
    15961 agcttttctt gattttcttt cgataggcga tggacctcat cgagaaaaag atagccgttg
    16021 ttggccgttg ccaataaacc atcggtatcc ttttcagccc ccgtaaaggc ccccttcttg
    16081 tatccaaaca gtgttgccga aagcagttcg ggattgtccg cataatcggc acagtttaag
    16141 atagcaaatg gtgcatcttt ttcaataatt tgctcatgga tagcgtattc aaaaattttt
    16201 gtggctaaaa aactttttcc aacacccgtg gctccattta ttaatacact gagtccgtta
    16261 ggcggatatt tgacagcggc tatacatttt tgaatgacct ctctcagact tccgtcatga
    16321 ccaactacag aatgaaaggc ttcgtttatt tgctccgtat tatctacaac cgcatattca
    16381 tctaaaggct tccacaaaac aggctttccc ggtaatttct cgaccttttt atcttccact
    16441 aatctcgtca aataatggct ggcattctga cgagagatac ctagattctt cgaaattttc
    16501 tcggcagtga cagaagacat attccctgtt agtcgtatca gctcatcaaa aactcgatct
    16561 ttagcgctct ttttcatttt ttcctccagt ttaatgtgtc taaaagcata ataaactatc
    16621 ggctgcaata tgtctattct atatttgtat tccaattttt actttgaaac acaaatagtc
    16681 gaatatttgc ctcagatatc tgacaaaata atttatctac caatttagtt tttatctaat
    16741 atgattttta tgttttttca tagc
//
LOCUS       DASHRQ010000060        16074 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.064, whole genome shotgun sequence.
ACCESSION   DASHRQ010000060 DASHRQ010000000
VERSION     DASHRQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.064"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            59..174
                     /gene="rrf"
                     /locus_tag="U2M88_002138"
     rRNA            59..174
                     /gene="rrf"
                     /locus_tag="U2M88_002138"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(227..697)
                     /locus_tag="U2M88_002139"
     CDS             complement(227..697)
                     /locus_tag="U2M88_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEM8374497.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(856..1314)
                     /locus_tag="U2M88_002140"
     CDS             complement(856..1314)
                     /locus_tag="U2M88_002140"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEM8374498.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1485..2629)
                     /locus_tag="U2M88_002141"
                     /pseudo
     CDS             complement(1485..2629)
                     /locus_tag="U2M88_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            complement(2642..3559)
                     /locus_tag="U2M88_002142"
     CDS             complement(2642..3559)
                     /locus_tag="U2M88_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEM8374499.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3987..4550
                     /gene="efp"
                     /locus_tag="U2M88_002143"
     CDS             3987..4550
                     /gene="efp"
                     /locus_tag="U2M88_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEM8374500.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4651..4821
                     /locus_tag="U2M88_002144"
     CDS             4651..4821
                     /locus_tag="U2M88_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374501.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            5036..6295
                     /gene="lysA"
                     /locus_tag="U2M88_002145"
     CDS             5036..6295
                     /gene="lysA"
                     /locus_tag="U2M88_002145"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEM8374502.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6282..7082
                     /gene="dapF"
                     /locus_tag="U2M88_002146"
     CDS             6282..7082
                     /gene="dapF"
                     /locus_tag="U2M88_002146"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEM8374503.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            7085..7966
                     /locus_tag="U2M88_002147"
     CDS             7085..7966
                     /locus_tag="U2M88_002147"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEM8374504.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7963..8679
                     /locus_tag="U2M88_002148"
     CDS             7963..8679
                     /locus_tag="U2M88_002148"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEM8374505.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8685..9380
                     /gene="dapD"
                     /locus_tag="U2M88_002149"
     CDS             8685..9380
                     /gene="dapD"
                     /locus_tag="U2M88_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEM8374506.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9396..10397
                     /locus_tag="U2M88_002150"
     CDS             9396..10397
                     /locus_tag="U2M88_002150"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEM8374507.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINENRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10394..11755
                     /locus_tag="U2M88_002151"
     CDS             10394..11755
                     /locus_tag="U2M88_002151"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEM8374508.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11715..12866
                     /locus_tag="U2M88_002152"
     CDS             11715..12866
                     /locus_tag="U2M88_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEM8374509.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12841..14010
                     /locus_tag="U2M88_002153"
     CDS             12841..14010
                     /locus_tag="U2M88_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEM8374510.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14185..15564
                     /locus_tag="U2M88_002154"
     CDS             14185..15564
                     /locus_tag="U2M88_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEM8374511.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15845..16074
                     /locus_tag="U2M88_002155"
                     /pseudo
     CDS             15845..16074
                     /locus_tag="U2M88_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5256 a         2839 c         3428 g         4551 t
ORIGIN      
        1 tgacttcaaa tccagttttg agtgaacaaa atttactcaa taaaagataa caccacagtg
       61 tggtggcgat agcgagaagg atacacctgt aaccatgccg aacacagaag ttaagcttct
      121 tagcgccgat tgtagtgagg ggttgcccct tgtgagagta ggacgtcgcc acgcaaaggc
      181 aaaaagatga actagaaata gttcatcttt ttttattttt attaagttaa agaacggatt
      241 caattgcagc ggtcattttc aatggagaag tttgtggtgc aaatcgttcg attactttgc
      301 catttctatc aatcaaaaat ttagtaaaat tccattttat tgctttagtg acagtaccag
      361 gagcttcatt tttaagataa gtaaaaagag gatctgtatt ttctccattt acgtcaatca
      421 attgatgcat agggaaggta acgccataag tggttttaca agcttctgct gcttcagtcg
      481 ctgaatctag ctcttgttta aattgattgg aaggaaagcc taagataacc agaccttgtg
      541 tctgataagc ttggtaaagt tcttctaatt gtttaaattg aggtgctaga ccgcattttg
      601 ttgcagtgtt aacaatgatt atcggtttgc ctgcatattt tttcaatgaa tagacagtgc
      661 cgtcttctaa agtagtttca aaattataaa tagacataaa gttttctcct ttctatttta
      721 tataataaaa agagcgctaa actgaaaact agctatttgt tttcccgttt agaactcttt
      781 tcagacaata aatatttaat aaaatacgaa gataagatag ctatattgct tctcatcttt
      841 acgttctttt agaaactatg cttctacttc agaccattca tcgcgttttg ctaaaaattc
      901 acgagccaaa gcttgtgcac cttctagtgt atggctggca gcccagccac attgtgtttc
      961 attgcttgca ggtacttcag tagcttcaag aacatctttc attgttgctt caagaacatc
     1021 taaaatgtct tcgtagcttt catgattcaa tacagtcaag taaaagcctg tttggcatcc
     1081 cattggagac cagtctacga tgtaatctgc gtgattgcgg attagttcgg cagtcaagtg
     1141 ctcaagtgaa tgtagacttg gcatgtccat gtgttcttta tttggttgtt taaagcggac
     1201 gtcgtattta atgatgacat cgccatgcgc acccgttttt cgatcagcta cacgtacata
     1261 aggtgctttg acttttgtat ggtcaagatt aaaactttcg acattcattc tcatttgtaa
     1321 gactcctatc tgtttgttca ttcgttttga tgcttttcac taaagaatta tttagtgaaa
     1381 tttacaaaaa cgtctttcta cttgattcta acacttatcg tttccaaggt gtagcaatct
     1441 gaaatatttt ttagattttt ctgctaagtc gtttattggg acttctagtt cttgattctg
     1501 tcaaatgcct gagctaaatc gtttatgaga tcctgaccat actcaattcc tacagaaaga
     1561 cggatcaagc cgtcctttat gccagcctct tctcgttttt cttttggaat agacgcatga
     1621 gtcatgacag aaggaatttc cactaaactt tctacgccac ctaaactttc tgctaaagta
     1681 aatagttgga gtgattcaac aaatggaata gcagattctt cattttttaa agtaaaagaa
     1741 atcattccgc taaagccgct catctgctgc ttagcaactc catggagttc atgatcgggt
     1801 aatcctggat agtagacctt ttctactgct ggatgagaaa atagaaaatc agctaccaca
     1861 aaagcatttt tttgatgttc ctccatacga acacttagtg tcttgattcc gcgctgaagt
     1921 aaccagctat cttggggacc aagtacggct ccgatagcat tttggtaaaa tccgatctga
     1981 tcggctagat cttttggtta gaagtcacta aaccagctac tacatcgctg tgtccgccta
     2041 gatatttggt accgctatgg acaacgatat cagaaccgag atctaaaggt ctttgaaaat
     2101 aaggagtagc aaatgtattg tcagcaataa ctatcaatcc atgttgatga gccagagtgg
     2161 cacttttttc taaatcagta atctttagaa gcggattgct aggtgtttca agataaagtg
     2221 cttttgtatt gggtttgatg gattgttcta ttttatcaag attactcgta tctatgatcg
     2281 tgtactcgag accattttta gtaagaactt tgtcaaatag acggaatgtt cctccataga
     2341 catcatctcc taataagata tgatcgccag cttgaaaaag agaaaaaacg gcatgaatcc
     2401 cagaaagacc agaagaaaaa gcgaatcctc gaacaccgcc ttccaaatca gcaatcagtt
     2461 cttccaatgc aaaacgagta ggattaccag atcttgaata ttcatattgt ttaggctggc
     2521 cgacgccatc ttgccgataa gtggatgttt gataaatagg tacactgaca gctcctgttg
     2581 tgggatcttc actgatgcct ccgtggatca attttgtttg aatgtgcatg tttctttcct
     2641 cctataagta aatgtttttt gataaatagc ggtctgctgc gtctggaaaa atcgtaacga
     2701 cacgatgacc aggtggtaaa cgacgaattt ctcttaatgc tgctgcaaaa gccgcaccac
     2761 tcgaacttcc aactaatagt ccttgttctc gtgctaactg cctagtatag ttaaaccctt
     2821 ctacatcaga gattgtctcg atctgattga ttgaaagggg agaaagaaac ggcggaatga
     2881 attctacacc gataccctca atttcgtgag gggctggatc accaccattt aacacagagc
     2941 cctctggttc tacaccgatt attcgaatgt cgggatattt ttctttcaaa aaggtagaag
     3001 ttccagcaaa ggttccacca ctcccaattc ctgctacaaa actatggata ttttctttga
     3061 tttcatgaaa gatttctggc cctaacgtat gatagtaagc agcaggatta tctttatttt
     3121 caaattgaag aggcagatag ctgtttgata tctcctctgc aagtttttta cttttgctga
     3181 tggcaccgat gattccttgc tcactaggag aatggatgat ctttgctccc aatgctttca
     3241 tcaattgttg tttttctaag ctaaacttct ctggaacaac aaaaaccgtt cttaatttgt
     3301 atgtgagtgc ggccagagcg atcccaattc ctgtatttcc ggcagttggt tcgatgatcg
     3361 tcgttttttc attgattttt ccttgggcaa atccttcttt gatcaaataa ctgcccaatc
     3421 tatctttgat acttcctcca ggatttaaat attctagttt tgcataaata gcagaacctt
     3481 taggcataga atagtgctga ttgtcaaagc gataaagcgg tgtggaacca atagcttctg
     3541 taattgtcga gataatcatg tgggcctcct aaaaaaagat aataaaaaaa ggagctgact
     3601 attaaatcag ctccttgaaa gacatagaga aaaattgagt agataaaaag ataagttacc
     3661 tggtactcat tctatgaagg gcaattaata gacatgacaa atagtatgct gcttttatgc
     3721 accatacaac agcaacgatt caattgtttt tggttgctga ctgtcatgtg tatccctcca
     3781 agtaaaattt ttattaacga tagcatgaaa aaaaataaaa gtcaaaggct tgaaagaaaa
     3841 aaatatttcg atagcatcgt ttacccgtgt ttatttttct gacaaatagg catgagacct
     3901 taaaagatag agaatactct agttttctgg ccggttgtct gatacaataa agttgattat
     3961 gataaagaaa agaaggagca aaaataatga tctcagcaag tgatttaaga gccggtatga
     4021 ctttcgtaca agacggcaaa ctgattaaag ttctggatgc aagccaccat aaacctggta
     4081 aaggaaatac agttatgcgt atgaaactga aagatgtccg ttcaggtgca acatacgaca
     4141 caacgttcag accagaagaa aaatttgaaa aagcacatat cgacacaaaa cctgtacaat
     4201 atttgtacac gatggatgat acagctttct tcatggattt ggaaacatat gagcaatatg
     4261 aaattcctgt agaaacagta gaagaagaaa tgaaatatat cctagaaaac atggaagtaa
     4321 aaatccaatt tttcggtagc gaagtcatcg gcgttcaatt accaacaact gtcgtattac
     4381 gtgttgtgga aacacaacct tccatcaaag gtgcaacagt cactggttca ggtaagccag
     4441 cgacaatgga aactggctta gtagtcaatg ttcctgactt catcgaaaca gatgaattgt
     4501 tggaaatcaa tacagcagaa ggttcttatg taaaacgtgc gtcaaaataa ttcgctaacg
     4561 gattgataag aatttgaaaa cagagcttga cctcttgaaa ggcagatcat cggatctgcc
     4621 tttcaagagg ttttttgtgg aatcagcaaa ttgaaaaata aaaaagtatt tgattttatt
     4681 tatattttga aagatgaatg gcaaaagcta gagaaagaaa aaactgcact tatttatgaa
     4741 gaaattgaaa tgattcataa aattgaaaat atccgaacta ttccgaacta tattgaagcg
     4801 aaaatatttt ttcatcacta aatagtgttg aatttatgtt tttatgtata aatagcttct
     4861 aatagttcgg gtattttgtt tttataacca tttttattct tatttttaaa aaaatatttc
     4921 gaaatatact agacaattca gaaaattttg tttaatatta ttccaataaa attcaaatct
     4981 ttatcaaatg aaattccaat aaaaacggaa aaaaagtgaa aagaggaatg aaaaaatgac
     5041 agaattgata attggcggag tcacagcaag cacattggca gaaaagtata agacacctct
     5101 gtatgtgtat gatgaagaaa agatgagaca aacgatgctg tcattcaagc gaggctttgt
     5161 atcgaaacgt tttgatacaa aagtgcttta cgcctctaaa gcattccaaa cgattgagat
     5221 gctgcattta gttcgtgaat atggattcgg gctagatgtt gtcagtggtg gggaaatcta
     5281 taccgctttg caagctgatt ttcctagaga tactatctac ttccatggta acaacaagac
     5341 gccagaggaa ctgatctatg cacttgaaaa tgatttgttg catattgttg ccgataattt
     5401 gatggaagtt gaattattag cagaattaag ccaacgctat ttaaaaaaaa tctgtatcat
     5461 gcttcggttg aatgtcggag tagaagcaca tactcatgaa tatatcgtca ctgcgcatat
     5521 tgattcaaaa ttcggcatga gctatgaaag taaagagtgc caacaagccc ttcagttgat
     5581 aaaagaaagc agctatctag agttagaagg atttcatgct catatcgggt cacaaatttt
     5641 tgatgttact gcttggttag ctgaaatcga caaattgacg aattacttaa ccgattttga
     5701 ccaacctctt tccttgaatc ttggcggagg gtttgggatt cgttatacac aaaatgatca
     5761 gccgatccca gtcgaagagg cactaaaaaa actgattgaa tatacggaag aagctttgga
     5821 aaaacgaaaa ctgaccattc gtcaattact gatcgaaccg ggtagaagta ttgttggaga
     5881 agctggtaca acactataca cgattggttt catcaaaaag acaccacata aaaaatatta
     5941 ctttgtagat ggcgggatga cagataatat ccgtccagct ctgtatcagg cagaatacga
     6001 ttgtgattta gcagcaaaac tgtcggaaga aaaaacagag aaagtgacga ttgcaggaaa
     6061 aatgtgtgaa tcaggcgatg tggtaattaa agaagcttat ttgccgaaag cagaatcggg
     6121 tgatctttta gctgtttatt ccacgggagc ttatggatac tcgatgagca gcaactacaa
     6181 tcgagcgaca cgaccagcag ttgtttttgt caataatggc caatcaagac tgattgttcg
     6241 cagacaagca ttttctgatc tactaagggg ggaagtttcc tatgatcctt cataaatata
     6301 atggctgcgg aaatgatttt atcttattag attacgcaga agacacagat tatagtcaat
     6361 tagccgcttc tttatgcaca aaagaaaaat atgatataga cggattgatc gccgtaaaaa
     6421 tcgatccgtt ggaaatgatc tattacaaca aagatggcag ccgcgcgccg atgtgcggaa
     6481 atggcattcg ctgttttgct cgatacgtaa atgatcagaa catgatgaat gaacaggaat
     6541 tcaacgtaga gacattagca ggcacaagaa aaattacggt gttggatccc tcaccttttt
     6601 attgcagcgt agatatggga gtgccagatg attctccgga aaaaatcaaa gctaatcaaa
     6661 cttatccaat aaaaaaccag gaaatagaga tcaatggtga aaaagtaacg atcactagtt
     6721 tatttttagg aacgattcat accgttgttt ttgttgaaaa tgcgcgtgag gaagtctgga
     6781 gaaaaagagg tgaactgatc tgtcatcatc ctttgttcaa agaaaaaacc aatgtcaact
     6841 ttgtagaagt agtcaatgaa caagaattga tcgtccgtac ctatgaaaga ggagtaggtt
     6901 ggacacttgc ttgcggtaca ggttgctgtg cctcttatgt ggttgctaag gaaactggga
     6961 aaatttcagc agatcaggta gttgttcact tggaacaagg agacttgatg atctctggtg
     7021 aggatgcgat acaaatggct ggcccagcag ttcatgaatg gacaagaata ttggaggaat
     7081 gaagatgata aaaggttcga tcgtggcgct gatcacgccg atgaatgaag agggatcagt
     7141 agattatgct ggtttggaaa aattgatcca attccattta gatgaacaaa cagatggatt
     7201 attagtttta ggtacaacag gagaatcttc tacgctcaca caatctgagg aggagcagat
     7261 ccttcaactg acagtaaaga aagtggctgg ccgtgtgccg gtgattgcag gagctggaac
     7321 gaataatacg aaagaaacga ttgaaaaagc gaagcatttt gcttctttgg gggcagacgc
     7381 acttttggtc atcacccctt actacaacaa aacaagtgat gcaggactag ctgctcattt
     7441 tacagcaatt gcagaagcct caccgattcc gcttattttg tataatgttc cttctagaac
     7501 aggcatgtcg attccaatcc atgtattagt aaatttagca gaacatccta atatcattgg
     7561 tttgaaagaa gcctcaggag atatggctta tgtgatggat gccgctcggt tgattggaga
     7621 agaattcttc ctttacagtg gaaatgatga tttgatccta ccagtaatga gtgtaggagg
     7681 aagcggtgta ataagtgtat gggcgaatat ccaacctaaa atagtccatg aattagtaaa
     7741 agatacgcag gacggaagat ggcagcaagc aaaagaaaag caattaaatg cacttgaact
     7801 gatccatgcc ttgttcagtg agaccaatcc tattcctgtc aaagcagcca tgtcattatt
     7861 agacttacct tccggacctc tacgcctgcc tttagtttct ctttcaaaag agaaaaagaa
     7921 acaattagct cagcttttgt taaaagaaag gacggttgaa aaatgaaaat cggactgatt
     7981 ggcagtggac agatgggaaa tagagtcgaa gaagttgcac aagaaagagg agatacggtc
     8041 cttcttttac ggacggcacc taaagaagca actacgcttg cttttacgga actgccagaa
     8101 ctgcttattg atttttctca tccagataat ttagagaaaa tcctttcgta tagcttatca
     8161 tatcaacttc cgttagtcat tggcacgaca ggttatacac aatcacagtt ccaagaaata
     8221 aaagaacatg ccaaacatgt acctgtgatg tatagtgcta atttttcgtt cggcatcatg
     8281 gtgatgaatc aactaatcaa gcaagcagct gcaatgttgc aaggctggca gatcgaactg
     8341 attgaaaaac atcatagtcg taaaaaagat gcaccatctg gtactgcaaa tatgctgctt
     8401 caaacaatcc aagaagtgca aaagctgcag cccgtataca actggcaagg gaaaaaaaga
     8461 gaagaaaacc aaattggtgt acacagtatt cgtgcaggtt ctttacctgg tgaacatgat
     8521 gtattgtttg ctgctactga tgaaatattt acgatcaaac atgaatcttt ttctaatcga
     8581 atctttgctg caggcgcagt agaagcagcg gactggttga aagatcagtc accaggttat
     8641 tacaaattag aagatttatt aatggacaaa ggggtgtaaa aaacgtggaa gctcaagaaa
     8701 tcatccgcta cattcaaacg gcatctaaaa agacgcctgt gaaagtctat ctgaatgtaa
     8761 ccgagccaat cgaatttaaa gaaagtaaag tttttggtga aggaagcagc ttcgttgttt
     8821 ttggtgacta tgagacgatt gctccggttt tagaagctga atcagaaaaa atcattgaaa
     8881 tcgaaatcga aactgctgca agatacagcg cagttccttt gctagatatt aaaaaaatta
     8941 atgctcgtat cgaaccaggt gctattatca gagatcaagt gtcaatcggc aacaatgccg
     9001 tgatcatgat gggagcgatt atcaatattg gtgcagtaat cggtgaaaac acgatgattg
     9061 atatgggtgc tgtcttaggt ggacgagcga cagtgggcaa aaactgccat attggtgcag
     9121 gagctgtctt agcaggtgtg atcgaaccag catcggccaa accggttatc gtagaagacg
     9181 gcgtactggt aggtgcgaat gcagtgattg ttgaaggtgt ccatatcgga aaagatgcag
     9241 tcgtggcagc aggagcagtg gttttagaag atgtggcagc agaaactgtt gtcggtggaa
     9301 tacctgcacg tgtattaaaa ataagtgatg atcagacaaa agaaaataca gcattgattg
     9361 ctgcgttacg cgaattatag aggagatgga gaaaaatgcg cgaaatagat acctgtattg
     9421 tgggggcaac cggattagtc ggccaaacga tgctcaaagt gttagaagaa tatgattttc
     9481 cagtaggacg attgaaatta ttagcttcaa aacgttcagc cggtaaagtt tgtgtattca
     9541 agaaaaagaa acacactgta caggaattga cagaaacttc atttgaagga tatgatttag
     9601 cgctattttc tgcaggtgct tctgtatcca aagagtttgc gccaatcgca cgaagtcagg
     9661 gactatttgt catagataat tcctcagcat ggcgggaaga ttcggatatt ccattaatcg
     9721 ttccagaggt caatctggaa gactatacat tgaatcggct gattgcaaat ccaaattgtt
     9781 caaccattca agcggtactt ccgcttaaag ctttacaaga agcctttggt atccagcgag
     9841 tcaattattc gacctatcaa gcagtttctg gttccgggca aaaaggcatt gaagatcttc
     9901 atcgtacgca aaatgacgag gaaccagttt tgtatcctta taatattagc caaacggtga
     9961 ttccggaaat cgatcagcct ttggaaaatg ggtatacaaa agaagaacag aaaatgataa
    10021 atgagaaccg taagattctg catcagcctg ctttgccagt atcagcaacc tgcgtacgtg
    10081 tgccaatcga aaatggacat ggagtctctg tggcagttca actgcaaaaa gagtttacga
    10141 cggaagaaat tagagagtgt ctcagccagt ttccagggat tcaattagaa gatgatctag
    10201 cagcacataa atatccgacg agcattcttg ctagaggaac agatatggtt tatgttggga
    10261 ggatcagaaa agacacttct ttagagaacg gattgttgtt ctatacgact gccgataaca
    10321 tacgcaaagg agccgctgcg aatgccgtac agattgctgc agctttaatg cagaaaatag
    10381 cggaggtggc aagatgagta atacgctgaa agtagcgaaa ttcggcggaa catcgatgtc
    10441 tcatgaagag caattcaaaa aagtccgtac aattgttctt tcagatccca atcgccaagt
    10501 gatcgttgtt tctgctttag gcaaacgtac gtcagatgat gacaaagtaa cagacttact
    10561 ttatttgatt tatgcttacc ttcagcatca tgttgattgg acaccccttt ggaaaaggat
    10621 ttgtgatcgt ttcattcaag taagagatgt tcttgccctg cagttaccta ttgaagatga
    10681 actagcacgc atacagaaac aactggaaga cgaaaagata actgaagatt atttagtaag
    10741 tagaggtgaa tatctgactg ctcaactgat ggctgagtat cttggttacc agtttattga
    10801 cgcaaaagac ctgattttct ttgactatga tgggaaaatc gatcaggaaa aaacagcaca
    10861 ggaaatcaca aaagcattta aaagatatcc gaaaatggtt gttccagggt tttacggggc
    10921 gaaccctgca ggaaaagaaa aattattggg ccgtggggga agcgacatta ccggcgcgat
    10981 actggcgaag atgttgcaag cggaaagtta tgagaattgg acggatgttt caggaatcat
    11041 gatggctgat ccgcgaatca tcgatcatcc taaaatcatc gaggaaatct cttttagaga
    11101 actgagagag atggcttata tgggggctaa cgttcttcat gaagcagctg ttttcccagt
    11161 tcaagaggct gatatcccca tccaaataaa aaataccaat gatcctttcg ctcaagggac
    11221 aaaaatatcg aatcacgaaa tagaaaagga aaatggactg acaggaatag ctggacgcaa
    11281 ggattttctt tccatcactt tattcaaacg tcatatgtcc gatgaaatcg ggtttatctg
    11341 gaaagcaatg agcatttttg ctaaacatga gatatcaatc gaacatattc cttcagggat
    11401 cgacaatatc ggagtgatcg tctcagcaga agcgattgcc gataagttat ttctcatcac
    11461 gaaagaatta aaagaaacac ttggcgtgga agaaatcgtc gtgatcgaag acttagcttt
    11521 gatctctgtc gttgggggtc cgcataaaga attgattgga ttatctggga aagtgttatc
    11581 gatattgaac aaattagaaa ttcgcacatc gatcttatca caaggagcac aggagttgaa
    11641 tctgatcatc ggtgtaccga ataatcaata tgaaacagta gtgaagggta tctatgaagg
    11701 gatggtgaat acggatgctg aaagaatatc gaatggcact ccatcaaatt cctgaaatcg
    11761 ggtttcatga attcaagaca aaaaaatatt tatatgaaca agtaaaaaac agcgaggccg
    11821 ttatccatga aatcggcgaa acaggactac tcctttattt tgataatcag caagaagaaa
    11881 cgattgcttt tcgaacagat atcgatgcac tgccagtcaa agaagaaacg aatctctctt
    11941 ttgcttctaa gcatgaaggt tatatgcatg catgtggtca tgacggacat atggcgatgc
    12001 ttttagggtt gactgacttt ataactaaac acattgagga tcttatttat aatgtagtac
    12061 tgatctatca gccatcagaa gaaatttccg gtggcgcaca aagcgttatt cagtcaggat
    12121 tgttggataa atatgaagta caagcaatat ttggctttca tctatggcct ggactagcta
    12181 agggagaagt tttctctcga ccaggacctt tgatggcaca aagcagtgaa acagacatta
    12241 tcattaaagg ccagtctgca catattgctt ctagtgaaaa aggaatcgat agtctggagg
    12301 cagctgtacg atttttgaag aatgtttatg attttgaccg gcaattaccg gcagagtgga
    12361 atcatctaat gaaatttggc caaatccaag gtggaacgat ccgaaatgtt ttagctggag
    12421 aagttattgt atccggaagt atccgttctt acagttcaga tatgcaaaat tatttgaaga
    12481 ggggattaga ccatttagca gaacagtttc actctacagc tactatttct tttcgctata
    12541 atgacggcta tccagctgta atcaaccata gaggcttata tgcagcactg ttagattcaa
    12601 acaaactaca tgaattagat gctccagttc ttcaagccga agattttggc atatatacgc
    12661 aacaatatcc atgtgtattt ttctttttag gtgtcggtga tacccctgct ttacatgatc
    12721 cgacgttcga ttttgatatg gatgttttag aaaatggtgt agaatggtac aaaacgattc
    12781 tttttacaga aaatcttctt gtaaaaatcg atagcagttc aaaagaggag cgagtaaata
    12841 atgatgaatt tatccaatcg atttagtcct aggctttcta gaatagaagt atcaaaaata
    12901 cgacaattcg atcagcaaat atcgtcgata ccagatgtga tcaaactcac cctgggagaa
    12961 ccggattttc caactcctga acatgtcaag caagcaggaa tagcagcgat tgaagaagac
    13021 ttttctcact ataccggaat gagaggatta gaggaactgc gggaagcggc ttgtatattt
    13081 cagcaacagc gttatggatt aacttatgat ccgcaaacgg aagttttgac gacagttggt
    13141 gcaacggaag cgattgcctc tgcgttgtta tctgttttgg aagaaggaga caaggttctt
    13201 attccagcgc ctgcttattc aggatatcag ccattagtgg aactagctgg tgctgaattg
    13261 atcccgatag atacttctga cacagggttt gtctgtcagc cggaacaatt tgaacgagca
    13321 tttgaacaat atggatcagc tgtcaaagca gtcattttaa actatcctaa caatcctact
    13381 gggacgacat tgtctgcaaa acaattgaaa gcaatcgctg aggttttaaa aaaatatccg
    13441 gtatttgtta taagtgatga agtatatgct gaattgactt atagtggaac gcatatgtcc
    13501 atagctactt atcttccaga acaaacaatc gtgataagcg ggttatccaa gtcacatgcg
    13561 atgactggtt ggcgagtggg ctttattttt gctcaaaaag cccttattga cgaactaatt
    13621 aaagtccatc aatatctggt gactgccgct acaacgatgt cgcaaaaagc tgcggctgaa
    13681 gcgctaataa atggggtgaa agatagcgag aatatgaaag agcaatatca aacacgaaga
    13741 gactatctgg tccagcaatt ggcgccttta ggatttgaag tgacccagcc aaacggtgca
    13801 ttttacttgt tttgcaaact cccagctgcc attcaaactg actcttggca gttttgtgta
    13861 gaattagcgg aacaggcaaa agttgcgtgt atcccaggaa ttgcatttgg cccagaagga
    13921 gaaggttata tccggatttc ttatgctagc agcatggaaa aattacatga ggcctgcaaa
    13981 cggatcaaag actttttaac agaacagtga ttcatagatt ttatcaaaaa aacaatcgtt
    14041 aattttgcgg tatattctgc aaggagacga ttgtttttta aatttagttc aaatgaacgc
    14101 gtttcttttg attcataact aatgtaatgt taagtttaaa ttgtttagaa tgattttaaa
    14161 agaatattca aggaggtttc atcaatggaa aaaatcaaag ttgtcattgt aggtgcatct
    14221 catggagggc atcaatcgat cttggaacta ttgacacgat acggtgaaaa tgtagatatc
    14281 acgctatttg aagcaggaga ctatatttcc ttcatgtcat gtggtatgga gctgtattta
    14341 gaagacaagg taacagatgt aaatgatgta cgtaatttca gaccagaaaa ctttccgcaa
    14401 tccaatgttc atattttaaa taatcacgaa gtaaaaacga tcgatgccga taaaaaaaca
    14461 gtaacggtta ctagcgcatc agataacgaa acaaaagaat atccttatga caaattgatt
    14521 ttgagttctg gtgtcaaacc aaacaatctg ccagtaccag gagcagatct agaaaatgtc
    14581 tacttgatgc gtggatacaa ttgggcaacc aaaatcaaag aaaaattaaa tgatccggct
    14641 attaaaaaaa tcgctgtcgt aggtgcgggt tacattggga tcgaagcagc agaagcaagc
    14701 cgaaaagctg ggaaagaagt cgtattgctt gatgtgatcg atagaccttt aggcacttat
    14761 ttagatgctg agatgacaga tattttagaa caacacctaa atgaaaacgg tattgaagtt
    14821 ttgacaaatg caaaaatcga agcttttact ggtaacggaa aagttgaggc aatcaaaaca
    14881 agtgaaaaag aaatcccagc agatctagtc attcaagctg ccggtgtgaa ggcaaataca
    14941 gaatggttaa aagggatcgt cgacttagat gaacgtggct ggatccaaac aaatgaatac
    15001 ttgcaaacga atcttccaga tgtgtatgca gtaggagatg ctacacttgc ttattctatc
    15061 ccagcacgca aaaaaatgcc gatcgcttta gcgactgttg cacgtagaga agcacgttat
    15121 gtagtaaaac atctttttga agaagtacca aacaaaccgt tcggcggagt cgtaggttcc
    15181 tctgcattaa gtgtctttga ctatcatttt gcagcaagcg gattgaatag ctttacagca
    15241 gcaaaagcag gagtagatgt acgagtgtct tattatgaag atacgttacg accaaaatat
    15301 gtaccagaag aacaaggaaa tccaaaagtg tccatccaat tattctcgga tccaatgacc
    15361 catcaattgc taggaggagc agtattatct acctatgatg ttacaggtca aggaaatgta
    15421 ttggcactag cgatccaaaa caaactgact ttagaagaac tagcagaagc tgacttcttc
    15481 ttccagccag gctttgaccg acaatggagc atgttgaatc tagccgctca acaagcgtta
    15541 ggagaagaac catttgtaga ataagaaaac gaaatatttg aaaagaggtt gtgaaaaagc
    15601 tgccttgcat caagtaataa gaacagccaa gtaaaaacag cttcttatgt ttttaacttg
    15661 gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattttcaa aaaagggact
    15721 gtgacacgat ttgtgtaccg acccccaaaa gttagaccaa aaaatctaac ttttggaggt
    15781 cggtattttt tatggttaaa tatacttttg aacttaaatt aaagatcgtt catgattatt
    15841 tggatggcaa aggcgattgg accgtaaaaa tccagatcaa gagattgaag cggaaatgct
    15901 taaaattcgg gagaagcaca aggattatgg ctgtttacgg atgaccaatg aactaagaaa
    15961 ccgcggtttc tctatcaata aaaagaaagt tcagcgcttg atcaagaagc taggaataaa
    16021 agtaacggca tacacgcgta aatcacgtcg ctacaattct tatcgaggaa aagt
//
LOCUS       DASHRQ010000061        15894 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.065, whole genome shotgun sequence.
ACCESSION   DASHRQ010000061 DASHRQ010000000
VERSION     DASHRQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.065"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(390..1337)
                     /locus_tag="U2M88_002156"
     CDS             complement(390..1337)
                     /locus_tag="U2M88_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEM8374512.1"
                     /translation="MHLEFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYI
                     EDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADY
                     IQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQK
                     AAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDS
                     VEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGI
                     CKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
     gene            1525..2712
                     /locus_tag="U2M88_002157"
     CDS             1525..2712
                     /locus_tag="U2M88_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEM8374513.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            2693..3997
                     /locus_tag="U2M88_002158"
     CDS             2693..3997
                     /locus_tag="U2M88_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEM8374514.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            4023..5036
                     /gene="citC"
                     /locus_tag="U2M88_002159"
     CDS             4023..5036
                     /gene="citC"
                     /locus_tag="U2M88_002159"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HEM8374515.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            5040..5333
                     /gene="citD"
                     /locus_tag="U2M88_002160"
     CDS             5040..5333
                     /gene="citD"
                     /locus_tag="U2M88_002160"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HEM8374516.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            5338..6243
                     /gene="citE"
                     /locus_tag="U2M88_002161"
     CDS             5338..6243
                     /gene="citE"
                     /locus_tag="U2M88_002161"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HEM8374517.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            6236..7795
                     /gene="citF"
                     /locus_tag="U2M88_002162"
     CDS             6236..7795
                     /gene="citF"
                     /locus_tag="U2M88_002162"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HEM8374518.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            7792..8349
                     /gene="citX"
                     /locus_tag="U2M88_002163"
     CDS             7792..8349
                     /gene="citX"
                     /locus_tag="U2M88_002163"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HEM8374519.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            8342..9205
                     /gene="citG"
                     /locus_tag="U2M88_002164"
     CDS             8342..9205
                     /gene="citG"
                     /locus_tag="U2M88_002164"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HEM8374520.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            9439..9848
                     /gene="tnpA"
                     /locus_tag="U2M88_002165"
                     /pseudo
     CDS             9439..9848
                     /gene="tnpA"
                     /locus_tag="U2M88_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(10485..11657)
                     /locus_tag="U2M88_002166"
     CDS             complement(10485..11657)
                     /locus_tag="U2M88_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEM8374521.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRIGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LIASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWITFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
     gene            12331..13143
                     /locus_tag="U2M88_002167"
     CDS             12331..13143
                     /locus_tag="U2M88_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374522.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
     gene            14778..15332
                     /locus_tag="U2M88_002168"
     CDS             14778..15332
                     /locus_tag="U2M88_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEM8374523.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            15586..>15894
                     /locus_tag="U2M88_002169"
     CDS             15586..>15894
                     /locus_tag="U2M88_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374524.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT         5564 a         2356 c         2950 g         5024 t
ORIGIN      
        1 cgtggagaaa taataagaac taccagtcag ataaaacaag ataccgaaga ctcactgcgc
       61 aaagggtggg gaatgaataa taaagttaga tagaaccccc tttttatagc ataaaaaaga
      121 tttagaagga tatatgatcc tattgcatga gaagtacaat aaaattaata agaagtaaca
      181 ttaacgaaaa aacgtggaaa ttattcagtg aatacaaaga aattaaatct tgaaaagtaa
      241 tggagtatgg cttggaaaag ttcaaaaaat atcaagacaa gcttctcaaa aaaagggatt
      301 ttgatatcca ataaaaagag tgaatcattg tactgccctc caaaaaagta gtaccaaaaa
      361 tctaaatttt ttggaggatt ttacatgact cactcttttt ttatcaaaga aagtattttc
      421 caagcagttt tttcatccaa aaataaatct gtaaaaaaat taccattcaa ggcgcctaaa
      481 caactctccg ctttgaactt acttttacaa ataccaattc gttttggaat atcgaaaatt
      541 tcttttaaag aaagaccaaa agtcttatcc tcagatgctt tgatgaattc accattgata
      601 ttgaagggtc ttccgtaaag aaatccaaca gtatccgtca tgcttggaaa taggatttta
      661 cgatgcttgt tccagggttc gacagaatca atagaggata aggtgccaat tccagaaatt
      721 aacatatctg catgcgaagc ggttgctaag gtattagcta aagaaggttc tagagccatt
      781 agttcacgtg cagtttcatt gatgacatat aaaggaatac tgagagtcaa ataagaagac
      841 tcataacgtt cagcagcttt ttgaactaat cgtgtggagc cagctaagga attatatttt
      901 ccgatttcgc caatgaattg agtaaaaatt aattcttcac gagcacccat tttaaactga
      961 tcaatcactt tgtaaacggt atctccccaa gatagtgcaa ccacattggc tttcttaatt
     1021 aattcttgaa tgtaatcggc agcaaatttt gaaaataata aatcgtgttg tgataagtct
     1081 tcactacttt ttaatatata gatattcgtt ttaaagtaat ttttgaagat ttcttctagc
     1141 tcataattcc tagcatacgg agaattaatt gaaatactaa ctaattgttt ttcacgtgca
     1201 tcttcaatat attttaaagc tttatatctg gtgatcccat attttttaga aatttcacta
     1261 atattgatat cctcgagata atagtcttca caaattttta caaggagtga ttcatagtca
     1321 gagaattcca gatgcatagt atagctccta actttttcaa atagatattt tttctcatta
     1381 taccaaaaaa attgaattta gtcttttttt aaatttttaa aaataagcat cattttttta
     1441 aaatttaaaa tgaaagggtt tttttatttt aaaaaaagtg ttaaagtggt tttgtaatta
     1501 aaaaatgtaa aaaggagtga aggtatgctt gatgaagttt tagaaatcca taaaaaaaat
     1561 gtaggtgttt tacggatgga aacaacagaa gccgtgcaag atgatcaaga tttagcaaaa
     1621 atgtacaccc caggtgtagc agaactaagt ttaatgattg agaaaaatca tgctttggca
     1681 agagaattaa caattagtgg taaattagtg gccgttatta cagacgggtc ggcggtatta
     1741 ggtcttggaa atgtaggaaa ccaagctgga ttaccaatcg tcgaagggaa agcgctgctt
     1801 tataaaaatt tagcaggtgt caatgcgatt ccattagcca tcgaacaaaa atcagtagac
     1861 gagattgtac aaacaattgt caatttgcaa aatagttttg cagggatcca tttagaagat
     1921 attgcagcac cgaagtgttt tgagattgaa gaaaaattac aagaaaaact ttctattcct
     1981 gtgtatcatg acgatcaaga aggaacagcg atagttgttt tagctggatt gatcaatgct
     2041 gctaagatcc aaaggaaaac attaactgaa cttcgagttt tgatcaacgg catgggagca
     2101 tctggggttg caacagctag actattaatc gctgctggta tcaaaaatct aacattggtg
     2161 gataaacaag ggagattaaa gaagcaagat agtacattaa ataaatatca aagagagttg
     2221 ttggatcata tacatgatac aaataataat gcaagcgttt tggaagatgc aatcgttgga
     2281 caagatgttt ttatcggtct ttcaatagga aatgttttaa cgcctgaaat gattggaaaa
     2341 atgaatgata atccgattat ttttgcttta gccaatccag taccagagat cacacccgat
     2401 ttagcaaaag aatctggtgc aaagctaatc gctacaggtt cttcgaaata tcctaaccaa
     2461 gtaaacaata ttttagtatt tcccggattg ttcaagggac tattactatc gaaagcgaat
     2521 aaagtcgatc tttcattaca acttgaagta gccaaacaat tagctgctaa ggtcgcagag
     2581 cctacggtag aaaaattcat ccctaatgtt tttgatgaag gagtcgcaaa cgtcgtttca
     2641 caggcagttt tggattattt acaaaaacta aaagaaaacg aggataatat acatgctgaa
     2701 aacattaaat agaattaaaa ttggttttat tcctttatct ctttatatca ttttggtact
     2761 cgcgtatctt gcaatgacga taagcaatat cgttccagaa aatgatatgt tagtttcttt
     2821 gggtattatg actgtttttg cttatttatt agaagaaatt ggtaaaacta ttcctatttt
     2881 aaaagattta ggtggtaaag tattagttgt tacatttttg cctgcatttc tagtctataa
     2941 acattggcta cctcaaagta ctgtaactgt ggtttctgat tttatgaata ataacaattt
     3001 cttgatgttc tttatcacac tactagttgt cggcagtatt gcttcaatga atcggcaaac
     3061 attggttaaa gcaacttcac gtattatcat tattttaatt ttatgcgatg tgattggttc
     3121 tttgatagga acaggtgtgg gaatactcct tgggctatct gcttggaatg cgtatttctt
     3181 tatcgttgcg ccgattatgg ctggtggtgt tggggaagga gcgatgccat tatcgattgg
     3241 ttatgcagca atattatctg gtatgggaca aaatgatatc tttgggaaga ttctaccgtg
     3301 tgtgatgttg ggaagtttag tcgcagttct actgtctggt atgctcaaaa aaattggaga
     3361 gaaataccca caattaaatg gtcagggtaa tttgatcgat ggggatgatg aacatttaga
     3421 gaacttgaag ggttcagcta aaccagtcaa tattgaaaaa acattgttag caggcgtggt
     3481 agcaataacg atttattttg ttgctgtcta tgttgagcac ttattaaaat cactccttgg
     3541 cttttctatt ccttcacctg tgatcttatt agtctctctt atgggggcta aaatgcttgg
     3601 ttttatccct aaaaatatcg aagaaggtgg aaattcgttg tatggattca ctgttcgtgg
     3661 gatcacacct cccttacttt ttggcgtagg ggttgcaaaa acagattggt tagagctgat
     3721 cgaagtattt aaaaatcctg caacgatagt aacaatcatt gtgacggtag tagctattgt
     3781 cgctacagct tttatcgccg caaaattttt aaaaatgtac ccagttgata ctgccatcgc
     3841 agtttcatgt tgtagcggac aaggcggtac aggtgcttta gctatcttag ctgcgggtga
     3901 tcgcatggaa ttaatgccat ttgcacaagt tgctgttcga ctagggggtg ctatgaccgt
     3961 aacgttcgct atctttttaa tgggtctgct atcataagaa cataaagaaa ggacgtgacc
     4021 atgtgtttga tcaaacagat atccaacgat tatttttaaa aaataactat atcaaagacc
     4081 aatgggaaaa tcttttgctt gattcaggaa tacaaaagaa gcaaatcgaa gattttcaac
     4141 atttagatca aacattgggt atataccata aagaaaagtt agtaggaaca gtatcttatc
     4201 aaaacaacgt gattaaatat atcgccgtgt ctgaaaaata taaagatggt ggacatacat
     4261 ttaatgtact agtctctgca ataattcagg caatgagtca acaagagatt tttcattata
     4321 ttgttttcac gaaaccgcgc tatcaaaaaa gttttaactt tttaggtttt gaaacaatcg
     4381 tatctacccc aaaaatagct gtattagaaa tgggagatag ttctataaaa cagtacctag
     4441 agaggattcc taaagtgaat ggtgaaaaaa aagcagcaat tgtcatgaat gcgaacccat
     4501 ttacagaagg gcattattat ttagtaaaac aagctgccaa taacaacgat tgggtatatg
     4561 tatttgtact cgaaaaagaa caaaatcttt tttcgacgaa ggaccgactc aaattagttc
     4621 aacaaggatt acaaaaactg aaaaatgtca ttgttctatc aggtggcgag tatattatta
     4681 gccctgcaac gtttcccacc tattttttac gagaagatga tgaaatagca aaggagcaaa
     4741 tggtcgtaga tgctacttta ttcaaagaaa gaattgccac ttatttagat atctctgcta
     4801 gatatgtggg agaagaacca ctttccccaa tgactcgttc atataacgac atactggaaa
     4861 cgattttacc ccctgaaata aaagttcatg tccttgcaag gaagaaaacg gaacaacacc
     4921 aagttatttc tgcttctcaa gttcgtaaag cgtatcttgc tgggcaattg gaaaaaataa
     4981 aatatatggt tccagaaaca acctatcaat acttaaaaaa taaaagggag cgttaagtaa
     5041 tggaaataaa taaatcagcg attgctggaa cattagagtc aagtgatgtc caaatcatga
     5101 tttcaccgaa tacagaagga atagaaatcg atttagacag tcaagtgatt gagcaatttg
     5161 gaaaacaaat caaaaaagtt atctatgaca cactagcagt ttatcagatt aaaaacgtaa
     5221 aactcaaggt agtagataaa ggggcattag attgcgttat tgttgcacga ttaaaaacag
     5281 cgatccatcg ttccttaggt atccaacacc aagaaacacc gtgggaggtt taattagatg
     5341 atgaaagaac gtttgagaag aacaatgatg tttgttccag gaaataatcc agcgatgatc
     5401 aaagatgcag gtatttatgg tgctgactcg attatgttcg acctggaaga tgcagtgtct
     5461 atggcagaaa aagacagcgc aagaatgctt gtggctgagg cattgaaaac agtggattat
     5521 ggtgacacag aattaattgt tcgaataaat gacctgcact cagaatttgg acgtgcagac
     5581 atttttgcaa tggttaaagc cggcgcgcat gtattacgtt tacctaaaac agaaacctct
     5641 gaagatataa cagaagtaga aaaagtgatt atagaagcag aaaactatca taatgttccg
     5701 atagggcgaa cattgatgat ggcagccatt gaaggtgctt ctggtgtatt gaacgcacct
     5761 gcgatcgcca aagcatctga tcgattgatt ggaatcgcca ttggggcaga agactatgtg
     5821 acaaatatga aaaccaaacg atatccagat ggaaacgaat tgttttttgc tcgcagtatg
     5881 atcgttcatg ctgcacgtgc agcaggcatt tccgcatttg acactgttta ctctgatgtg
     5941 gatgatatgg aaggattttt aaacgaagta cgtttaatcc atcaattagg ttttgatgga
     6001 aaatcttgta tcaatccacg gcaaattcca gtagtcaata ctgtttatca gccgtcaatc
     6061 aaagaaatca aacgatcact agacatcatc tcggcaatcg aagaagcaaa agaaaaaggt
     6121 tctggcgtaa tttctttaaa tggaaaaatg atcgataaac caattgtaga acgtgcacaa
     6181 cggacattga tgttagcaaa agcatcacga ttgattgatg aggagggaaa tatccatgca
     6241 taataaacaa agacgtagca ttccatcaag tgttttaaca aagtataaaa aagaattcca
     6301 aagtgtttca ttatcgccac aaacgattca acgttgtgca ccaaaagtaa cgactgtaac
     6361 taatcagaca aataaattga tgcactcgat caaagaagct atagaaaaat cgggcttaaa
     6421 agatggaatg acgatttcat ttcatcatca tttgagagaa ggtgattttg tttttaacca
     6481 agtgatggcc gtgatccgtg aaatgggcat caaagaattg accttagcac caagttcttt
     6541 aacgaacact atgaatgaga tggttattga atgcatcaag gacggtacag taactcatat
     6601 cacaagcagt gggatgcgcg gcagcttagg tgaatttatt tctcatggag gaatggacaa
     6661 tcctgtgatt ttacgttctc atggtaatcg tccacgtgca attgaaagtg gtgaattgaa
     6721 aatcgatgtt gcatttctag gagtaccaac atctgatgat tatggaaatg ctaatgcgac
     6781 tgttggaaaa tccgtttttg gttcattagg gtatgcaaaa gtagatgcac aatatgcaga
     6841 taaagtcatt cttctaactg atcatttagt tccttaccct aatacgccca ttagtattcc
     6901 gcaaacacaa gtcgactatg tcgtggaagt agaagcaatt ggtgatccga ataaaatcgg
     6961 tgctggtgct acacggttta ctaaaaaccc aaaagaatta aaaattgctg aaatggtcaa
     7021 agatgtcatt gttcactcac cttatttcaa agatggcttt tcttttcaaa caggaacggg
     7081 tggtgcagct ttagcagtaa cgcgttactt agctagtgaa atggaagaaa aacagatcaa
     7141 agcttctttt gcattaggtg gcatcactaa accaacggtt gatttattgg aaaaaggtct
     7201 tgttgaaaaa gtgttagatg tccaagactt tgacaaagga gcagctgaag gaatggcgcg
     7261 gcttgagaac caacaggaga tcgatgcttc cttttatgct gatccaagta ataaaggagc
     7321 ggttgttaac caattagatg tcgctatttt atctgcatta gaaattgaca caaacttcaa
     7381 tgtaaatgtt atgactggtt cagatggtgt gctaagaggt gcgattggtg gacatcaaga
     7441 tgcagcaacg gctaaactta ctatcatctc agcgccgtta gtacgtggcc gaatacctac
     7501 agtagtaaat gatgttacta cattgatcac accagggaaa agtatcgatg tattagtcac
     7561 agaagtaggg gttgctatca atccccaacg taaagatcta atagctattt ttgaaagaat
     7621 tccacaaatt cctgttttta caatagaaga attacagcaa aaagcagaaa aaatagtagg
     7681 catcccggaa cctttgcaat tcacagatcg gatagttgcc tatgtagaat accgagatgg
     7741 ttcaatttta gatgtcgttc gtcaggtgaa agaatttgaa gaagagagat catgatgttt
     7801 gatactggag aaattcaaga aatcgaggat attttgatcc ataaagaaca acgtgctaac
     7861 ttgcaaagca acctgctttc caagtattca aatagttcat tgatggtctt tacattgaat
     7921 attcctgggc cgatcaaaaa taatccaatg attgagcagg tatttcgtca gggacagcag
     7981 tcaattcagc aaatgttttt aaaaaaaaag gtggaaatga cgtacacaaa agaaatcaat
     8041 ttgccaagtg gattggactg gtacgtaata gtaacagaag cacctttgaa tctcaaaaaa
     8101 tggttggtca cgttagaaga gcaagacgca ttaggtagac tttttgattt agatgtcttg
     8161 ttcaaaaaag gagagcagat aacaattgtc agtcgccaag ctcttaatca tggggaacgt
     8221 ccttgtttta tttgtgggaa atcagcaaag gtatgtgcac gtaatcgtac acatactgtg
     8281 aaagaaatgc aaaagaaaat cgaacaaata gtagccgatt ccttaaaagg agcattgaaa
     8341 catgcatgat aatattcaaa aaatagcaca agaagcacaa ttagcgatgc tttatgaagt
     8401 tacatgtatc aataaaccag gattggttga tccagtagac cccgggtctc atcaagatat
     8461 ggacattttt acttttctac aaagtagtgt agttttgact ccctattttg aagaatttat
     8521 ccaaacagga ttggaccgac aacatcaacc aattgaagaa acctttatgg cgattcgaca
     8581 gattggttta gaggctgaaa ctgcgatgtt ctcagtgaca aatggtataa atacacataa
     8641 aggtgctatc ttttcattgg ggatttttct tgctatttgt ggccggttaa ctatttggaa
     8701 agttccttgt aaaaaaagtt tctttcaaaa agaaattcaa aaaatgacga aaaatctact
     8761 taacgatttt gggaagattg atcaaacaaa accaaaagca tggacttggg gagaatatct
     8821 atttgttaac catggattga cagggatacg aggggaagcc aaaagaggtt atccttgtgt
     8881 ttttgaccga ggattgcctt attatcaagc atatcaaggg tcgcaacaag ataagatgat
     8941 agacacttta ctttttctat caatggaagt agaagatacc aatttgatca aacgaagtaa
     9001 taatagacat gtttttgaag agtatcaatt acttattcgt ccttattttg aattaggggg
     9061 tgtaaaaacc atacaaggta aaaaatattt ggattaccta aatcagcaat ttaaaaagaa
     9121 aaattggagt attggtggaa gtgcagactt attgatcctt acaatctttt tagataaaat
     9181 catggacaaa ggatggttat gttgatacta taatacatta ttttgtatta gtaagctaat
     9241 aactaaaaaa tggttcaata caaatatttt ataaaaaatg gtagtgccta tcctttcaga
     9301 ttgtagacaa agaaaaattt ctttgtctac aatttttttg ttaatgactt cagtcagttt
     9361 cgtgcgcaaa gcgtgcgaaa caagaaaacc atgcgtttct acacgtggaa gaactagcat
     9421 atcgaaagga gttttttcat gtcaaatgac gataaaagtt tagcccatac tcgttggaat
     9481 tttaagtatc atattgtatt tacaccaaaa tatcgacgaa aagtaatcta taggcaatta
     9541 cgaaaagata ttggaaaaat acttaggaag ttgtgcaaaa taaaaaacgt ggaaattata
     9601 gaagcacatg caacgccaga tcatattcac atgcttgttc aagtaccatc aaaaataagt
     9661 atatccagtt ttatagttat ctgaaagatc gtagtgctgt gctaatccac gagcagcacg
     9721 taaatcacaa atatagatac ggaaataaat cattttggtc ggaggaatat tatgtgagta
     9781 cagtcgaatt gaatcaaaag acaattgaaa aatacatccg tgaacaagaa gctgatgatc
     9841 gcatttgaga tagtataagt aaaagagagt agatgttgca ataacaaaag ggtaaaaatg
     9901 gactacctcc agtaaaatac cgaaaaatag tttaactttt ggagataagt ccatttaagg
     9961 gtctatagaa tgacaatttt tttaggggat tttactatta ttacttcaag acccgcttgg
    10021 attcgcaaag tgagtgaact cttaaaacta cctgatagta ggttgtttta agagttatct
    10081 gaaagtaaaa attgcattta tatttttgat aaatacgtaa atttatatat acgtatttat
    10141 caaagtttta ggccaaagga atattgtgta aatacactag gattgaatga atagaccaga
    10201 agaaattgcg tccaagaaca aaagaagcat gatagtgctc tatataaatt gggcattcaa
    10261 gaatctgaaa tttaaaggta gaattttagt tagactttta gattttaaac acaaaaataa
    10321 ccatacaagc aacaagaagc aaagtgctat attataaatt gtccgtccgt ttacttacat
    10381 aagatcattg gggctatttt taaaagagaa taatagataa taaatgaaaa aaaagtcaag
    10441 tccacaatcc tgtgtaaaat actatacaat gctctcacta ttttttactt ggcaaattta
    10501 atatattttc ttgatgaaga aagccactca tcgtgttgat caataaggac tgctccaatt
    10561 aaacgattgg ctgaaacgca gtttggaaag atacgaataa ttttttctct tctgcgcact
    10621 tcttgattca gtcgttcaat tagattggta ctctttagtc gattgtggga atttccttgt
    10681 acggtatatt gaaaggcgtc ttcgaatcca tcatccaatg atgcgcaagc ttttgaatat
    10741 tttggttgat cgatataatc atgaatcaat cgatttttag cctcacgagc taagttaata
    10801 tctgtgaact taaaaattcc tttaacagct tctctgaaag attttgaatt ttttttagga
    10861 atggtggtaa agatatttct taggaagtga acttggcatc tttgccaact tgcgttggtg
    10921 aaggattttc taatggcaga gactaatcct ttgtgcgcat cagaaataac gagttctgta
    10981 ccttgtaaac cgcgttcttt taggtattca aaaaatgtta tccaggtctc ttcgctttcg
    11041 ccactttgaa tcatgaagcc gataatttca cggtcgccat ctttggttat tccaatcgct
    11101 atatgacagc tttttgagag tactcgattt tcttctcgta cttttatata gagtacatcg
    11161 gtcattaagt aaggataatt tttttctgat aataaacgat tctgccactc gttaaccatt
    11221 ggttctagct gttctgttaa gctagaaacg aaggacttag agacggattt accacaaagt
    11281 tcttccacaa tttttgatac tttacgagtt gaaacgcctg atacatacat ttccaacatt
    11341 gaagcaatga gggctttttc gtttcgttga taacgttcaa acactgtggg tgaaaaatgg
    11401 ccatcacgtg ttctgggtac ttttaattct agcgtgccta tacgtgtcgt aaagctgcgc
    11461 tcataatagc catttcgttg actttgtcgg ttttctgttc gttcatattc ttttgcttga
    11521 atatattctg ttcgttgatt ttccattagt tgattaaata ccgttgttaa aatattttta
    11581 gaaacgtcat cctttacaga atattcaata atgctttgaa tctcttcgct tttcagtgta
    11641 aaatgtactt gggtcatgta aaagtcctcc tgggtatgtt tttgtcgtta aaaacattgt
    11701 accgtaaaag gactgttaca tggccttttt acttttacac aattataagg actttatcga
    11761 aaaaaagcag ttatttgtta aaatcattga tattattaat aataagtatt ttttgaaagg
    11821 ggaaatataa aaggtttttg aaaataaata aaaaatagta ttacttgttc tattgctagt
    11881 tattgctagt ctttattctt ttttgttttt tagggatgta caaaagatct caaccacagg
    11941 agacattgct tttcactttt ctagaataaa atgcttgtcc tcagtatttt ctggtccaat
    12001 aaattttacc acatttaata gctatggcgg aggcgtgaat ttttttatcc atatctaaca
    12061 tctttccagc cgtaatattt tactggacaa caaacaattt gattctaagt tatatactta
    12121 ctatacgttg ggatactgaa tatctgtaca attctagtga gctatcaata tggacaaaag
    12181 aatagatgca gccttcttat tcagctgttt gtatagcttt tatggattga tatatattag
    12241 cgtactgcaa tttcagaatc aattgcgttg acgattgtac cagttgtctt gtattacaca
    12301 tatactcttc tttatgaaag aaaaaactgc gtgattcttt tagctgtcag tatgagtttg
    12361 ctcatctata cccatattct ctccaccttt atgagtgtga ttgttattgg cttcttgttt
    12421 atcggctctc ttttctctaa gcggaaagag agaaaagaaa tgtcgaatct gatatcaaat
    12481 atgataaaag ctgttggttg gaccattctt ttaacagctt acgtttggta tccgatgtta
    12541 caacaagtcc tacatcaaaa aattaatcgt ccttttcgca caaaacttca agaaagggca
    12601 ttaaacattt ctgatagttt aatcggtgca gtaaataatg atttagttac atttacaatg
    12661 ggaacaattg gtgtggtttc atttattctt ccacttttct ttaaaaaaaa atttgccaat
    12721 aaagaaaaga ttattaattt ttgtgcagtg accacttggt tcttatcaac gaatcttttt
    12781 ccatgggaat ttttacaaaa aacacctatt caaatcattc agtttccttg gagaatcttg
    12841 ggttttcaag tgctttttgg ttcattgatc ttaattatag tgtttttaaa gtggaaaaca
    12901 agcaacaaaa aaagtatgtg tagccttgta ggaatagtcc ttcttatttt tacagtgact
    12961 gtagcaacag aagcgaatta ttcgcagaaa attcaatcgt ataaaggtcg tttaattatg
    13021 accaaaaaag atgttacact ttatacaacc tcgagaactg gaggtcttta tgactatgca
    13081 ccattagatg cattgaaata taaagatcat ttaaagaaac atgaagtaaa agtctatgat
    13141 tgatgggaga atgcatctca taaagcacat aattctaaaa taatctatac agtaggagat
    13201 gcgaatgagc aaaatatcac gttacctgta tttaattact gggaacagtt atgaaagtaa
    13261 atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa ggtacgggaa
    13321 aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta tctttattaa
    13381 ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc tgcggttaaa
    13441 tgaaaataga ttagatagat attataaata tgttgattct attttcatag caatggagaa
    13501 aatacattag caatatataa aagaaggtat caagagtcac ggtgaattta ttccgaaaag
    13561 tgaaaagaac aaatataaaa tttaataaaa atatagaact aaataggaaa acgtagacgt
    13621 aattttctac gttttccttt tcatatatat taaatattac ggaaatttat ataaactaat
    13681 ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt taggtctatt
    13741 taactcatca gacattctat gctcattttc tccaaatttg tttcaactct ttcgtactta
    13801 attttgcgta tttgaaaatt cttcttttag agtactttta ttacgatact agtatgatat
    13861 atatctatag caaacagtta aaatcttcct aaaataagcg aatagcattc ctaaaaccta
    13921 taaaattttt agatgaagcg acaagtggac aattaataaa ctacttgaac atgaaattat
    13981 ggatcacctt tcaaatttag aaaatggaat tcttatactt attgcgcaac atttattgat
    14041 tgcaaaatta tgtgacaaaa agaactagta gaagaaggaa taatggcttg ataataaagt
    14101 actacctatt aaaggtagta ctttatttaa tttagtataa attaacatac tactatattt
    14161 taagtttcat attatataaa ttaataatat actaccattt tttggtagta taatttaaag
    14221 gtaataagtt attcttttat tactatgttt gggttttata gcaaggaaag tttaataaga
    14281 gtttggacct agcaaacaat attaatgagg tcattaaatc ttattatctt tagtgatata
    14341 acgaaatatc tacacagaca aaattatgat cttgcgtaaa gttataaaat attaataaaa
    14401 atattttatt aatgaaaagc ttggtagcac ggttattcat atgctaattt atttcacttt
    14461 atagtaaggg gagttcttag tatattgtcc aaatttaaat ttcatataat ttaattatgt
    14521 aaattagcta ttctaagaac ttctacggat aactgcccgt ctaaaattta attaagagct
    14581 tagaatcttc atataagtaa ggttaatttt tttgtacaaa atagtcggaa tattctgagt
    14641 tactgaaatg atataaaatc ataaactaaa ccccttgatt ttcttcactt ttaacagagt
    14701 atatatataa gttcatgatt atggtaaaat atatatataa tcatgaactc agaatatttt
    14761 taagaaggga aacaatagtg agctataatg tacaaccgtt aaggacaaat gaagagatac
    14821 aagattttca gttttggcta agaagaacaa gtaatccaga aagagatagt tttttatttt
    14881 tgtttggaat caataatggg ttacgtatgt cagatattat aggattaaaa gttggagaca
    14941 tacgaggtaa aagtaagcct attattgttg aacataaaac aggtaagcga aaaccgattt
    15001 ttatagataa tttgagagaa gagattttat tatatactga aggtaaagaa gaaaatgatt
    15061 ggttatttcc aagtcggcaa caaggtcgac atataacaag ggatcgtgtc tatcagatat
    15121 atgcagatat agcagaaaaa ttaggaagag atgatatagg aacacataca ttaagaaaaa
    15181 cttttgggta tcactactac aagaaaacta gagacattgc taccttaatg tttattttta
    15241 atcatagctc gcaagcaatt acaaaaaggt acattggtat aactgaagat gaaatagggg
    15301 catcgttacg tggttttaaa ttgggaggct aattgagata ttaggatata ttttttgtgt
    15361 ataatgaaaa cttagaaggt gagattaatg aagataactg aattttcgga agtatttaaa
    15421 acgtttagta acatttacaa tagaatacca gagatagaac aaaatgttgt agttgataat
    15481 gattattata tagagaatga ttttaataaa ccaagaaaat atgacaacct atacaatttt
    15541 atacacaata gaacaacaat aaataacaac agtaacactc gagttttgat taattcagca
    15601 tattttcata ttaaatattt aaagaaatat gagtatgaag aagttgtatt agattatggg
    15661 ctaaatagac agtcattttt aatttatgca ataaataacg gaaatttgcc aagcaataaa
    15721 aagaacataa ttattgaaac atatgcctat ctggaggggg gatctaaaaa actaattaag
    15781 tcatgtaata tagagtatca attatcagta ggagaaatta aatgtgtttt cgaagaagat
    15841 ttagaacttg acttaaaaaa aatttttgcc agcattcatg attatatagg tttg
//
LOCUS       DASHRQ010000062        15605 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.066, whole genome shotgun sequence.
ACCESSION   DASHRQ010000062 DASHRQ010000000
VERSION     DASHRQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.066"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(190..567)
                     /locus_tag="U2M88_002170"
     CDS             complement(190..567)
                     /locus_tag="U2M88_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374525.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(867..1748)
                     /gene="galU"
                     /locus_tag="U2M88_002171"
     CDS             complement(867..1748)
                     /gene="galU"
                     /locus_tag="U2M88_002171"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEM8374526.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1791..2810)
                     /locus_tag="U2M88_002172"
     CDS             complement(1791..2810)
                     /locus_tag="U2M88_002172"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEM8374527.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(3443..4447)
                     /locus_tag="U2M88_002173"
     CDS             complement(3443..4447)
                     /locus_tag="U2M88_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEM8374528.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(4460..5509)
                     /locus_tag="U2M88_002174"
     CDS             complement(4460..5509)
                     /locus_tag="U2M88_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEM8374529.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(5526..6347)
                     /locus_tag="U2M88_002175"
     CDS             complement(5526..6347)
                     /locus_tag="U2M88_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEM8374530.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(6334..7113)
                     /locus_tag="U2M88_002176"
     CDS             complement(6334..7113)
                     /locus_tag="U2M88_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM8374531.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(7130..7600)
                     /locus_tag="U2M88_002177"
     CDS             complement(7130..7600)
                     /locus_tag="U2M88_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8374532.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7612..8031)
                     /locus_tag="U2M88_002178"
     CDS             complement(7612..8031)
                     /locus_tag="U2M88_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEM8374533.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(8112..10889)
                     /locus_tag="U2M88_002179"
     CDS             complement(8112..10889)
                     /locus_tag="U2M88_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEM8374534.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            11080..11304
                     /locus_tag="U2M88_002180"
     CDS             11080..11304
                     /locus_tag="U2M88_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374535.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            12083..13798
                     /locus_tag="U2M88_002181"
     CDS             12083..13798
                     /locus_tag="U2M88_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8374536.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            13801..15564
                     /locus_tag="U2M88_002182"
     CDS             13801..15564
                     /locus_tag="U2M88_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8374537.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         4569 a         3218 c         2659 g         5159 t
ORIGIN      
        1 acattggtat gatttatata tatataaata acataaaaaa aggaatggct tatcccgata
       61 caacaagcat tcctttaaaa aagattcttt tatataagtg aaaaaagctt ttttccacca
      121 attaaataaa tctctcatct caatagttta tccattattt tatctttttt tgcgtatacc
      181 tactaaatat taccgtcttc ttctcgttct tctactttgt tcgagagaat ctgtactaga
      241 aaaagtagtt gtatgtctac cttttctgcc tatccgagac tttcttttca tatatgggat
      301 ataaatgatt agatacagta cagcaaaaaa taaagcaaat acgcaagctt cccaactacc
      361 taacgccaat tcaaccatca aggtagcaac cccaacggat gctgcatacc agataaaaat
      421 cagaaacgtt tctttttttc tgaccatgcg aaacatgata aatagactaa taagcaatag
      481 tggcaacaca aatatccaat tgcttccttc catcagccac ttgagtatat caagcagcga
      541 ctgtgcaaca gcgactaatt ttcccatagt gaacacacac ctttttttat aattaaaaaa
      601 ttcgcctttt tattctacta tgaatcttct ggcttggctt tcatttctct tatgtaatca
      661 ttttctttta tacaacttaa aaaaaaatta aaccattagc ataatatttt ctgaatctat
      721 tcacgaaagt ctttttctat ctttcgtttt ccacgactca actgatctag agtcaaaaaa
      781 ctaaaacgac agttgcgcaa atgacgcaca agctgccgct ttaataacaa atagcctttc
      841 attctcagca ggccacttat ttccagttaa ctttctgttc ttagcttttt actcaactgg
      901 ataatatatg cacttaatga agtcccaatt tccgaatgtt gaagtccata ttcaatattt
      961 gtcttcatat atcctaactt atcgcctacg tcgtatcttt ttcctttaaa ctctctcgca
     1021 tagaccgcct ctgtctggtt aagagtctga agtgcatccg ttagctgtat ctcgtttcct
     1081 gctcctggtt cttgagtttc taatatctca aatattttag gagtcagtag ataacgtccg
     1141 ataatggcta aattacttgg tgcttttccc atttcaggtt tttctacaaa cccttttact
     1201 ctgcaacgac ccttttcgct atctgtttta ggatcaataa ttccgtattt agatgtttca
     1261 ttttctggta cacgcatgac ggcaacagcg gaagcttgtg tttcttcata atccatcatc
     1321 aattgttgag tcaatgggat atcatctttc atcaaatcat cccccagcat gacgacaaac
     1381 ggttcatttc ctataaatgc ttttgcttga agcacggcat cccccagtcc tcttggatga
     1441 gattgacgga tgaagtgaag attgacttga gtcgtttctt caactaaagc gagtaattct
     1501 gttttttcct tttcccgcaa attgttttcc aattcgatat ttgcatcaaa gtgatcttca
     1561 atgggtcttt tttcttttcc cgttacgatc aagatgtctt cgattcccga atccaatgct
     1621 tcttccacaa taaactggat cgttggttta tccacgattg gaagcatttc ttttgccatg
     1681 gcttttgttg ccggcaaaaa acgtgttcct agcccagcgg caggaatgac tgcttttttg
     1741 accttcactt gctgttcctc ctaaacgacg attaactatt gatcatctat ttaacgaaga
     1801 gaaaattcat tctccatttt tccgtcacgt aacatgattt cttttgctgc ttttttgata
     1861 tcttctccat tatacaaaac attatagatt gtttgagtaa ttggcatctc aacacctagc
     1921 tgttgagcta gctctactgc tgcttttgtc gttgccacac cctcaacgac cattcccatg
     1981 ttttctagaa cttcttccag cttatgtccc tgtcctaaca agttaccggc acgccaattg
     2041 cgagaatgaa cacttgtaca agtaacgatc aagtccccca caccactcaa accgataaat
     2101 gtcagaggat tggcacccat cgcgacacca agacggctga tttctgccaa tcctcgagtc
     2161 atgattgctg cttttgcatc atctccgaat cctaatccat ggatagcgcc ggcaccaatt
     2221 gcaatgatat ttttcagtgc tgcaccagtt tctacgccaa tcacatcatt atttgtataa
     2281 atcctgaaat aatcattcat gaacagtttt tgtacatagg ctgctgcttt ttgatcaata
     2341 cttgcagctg taattgttgt gatatcatgt actgccactt cctcagcatg gctaggacca
     2401 gataatacaa caattgcctg tcttttttct tctggaattt cttcagccaa aacttctgag
     2461 attcgtttat gcgtatcttg ttctagtcct ttacttgcat gtataatgat tggcttagta
     2521 ttcattttac caataagttc ctgtgcgact gaacgaatcg cttttgttgg aacaacaaat
     2581 aaaatagcat ctgcgttatc cactgcttct gccaattctt tatatccgat aatcgattct
     2641 gggatcgtca aatcaggtaa aaaatgctga tttgtatgat acttattgat ttcatcaatc
     2701 tgttcaggta catttcccca aatacggaca tcatgcccgt tttctgccaa tgtttgtgct
     2761 aaagcagtgc cccaagatcc aggaccaaga actgcgattt tttgtttcat ggatattcct
     2821 ccaaataaat aattgataat ttccattata gtatacattt cttaaagttg ggactaattt
     2881 ccattgtttt taagtgtttg tttacttctt ttatcgtttt tacttgaaat aatcaatgat
     2941 atgttacaat agtctacatt ttatttttac actcgttatg ctttgcttgt tcctaagagg
     3001 aagcagtttt gacaatgtta ctaccagcat tactattagg aggttaggaa acttctccta
     3061 gtgagtattg atttaaaata cataagagac agattgaaca tttctaaatc tgtcaggtcc
     3121 tgcaacggac agattgtctc tgttgacggt tgacatagga acttttgttt tagtcaacat
     3181 tctggataca aaacgctatg taaactggcg caactcgttg tacttggact tttactacgg
     3241 atccatgcaa caactaaata gtactattgc ataacaaaaa atggttggca cccacatgtc
     3301 ccattaaaca gattataagt tgaactgtag ataatgcaaa aaatgtgttt tataacgtaa
     3361 ttgatgccaa tattttaaaa aaataaaggg agcagttatc gctgctctct ttatttttta
     3421 tattcttttt atccagtcta tcctaataat caaactgacg atagtagcgg cgtatctcta
     3481 atggatggca gttcaactgc tccacatgag catcgatgcg ttgtgtcact acatgcatca
     3541 ataagaaaga caaaacacgt ccgcgatatt tttcactgat accttctact ggatagtctt
     3601 ttgtgtctat caatgtataa ttgctgcaaa tttttggcaa aagtttagcc acacgttcag
     3661 ataacactct ctgttcatct tcgcctaaaa ataatgtgac tggggttgtt tcttctacga
     3721 tctctaacgt cccgtgaaga aactcggcag catggataga tttcgatgga agccagcttt
     3781 gttcttccca gtaacacatg gcatatgaat aaacagctcc ccattgattc cctgctccaa
     3841 taaaataatg catactatca cgtcgatgtt tttctgcaaa tgataagcca aatgcatctg
     3901 cttttttctc tgcttccact aaccctgttg gtaaataacg ttcgagctgt tcatagtatt
     3961 ctgaataagc ttcgaattct ccattcaaat acatcaatcg atctgctgcc ataaaaaatt
     4021 tgatttgttc tgttccagga gctggataag tgaccacaaa atcgcatagc tgagacaact
     4081 tactgttcgc tttatcgata aatccaatca gtgtcgctcc tactttcttg atttgttcta
     4141 ctgctttcac tacttcttga gttgtaccgg taacagagga caaaataacg atagaatctt
     4201 ttgttaatct tttgtttcca gtagtgtaat attctgcagc atgctgtaca aagacaggca
     4261 agttgctttt cccattcata taagtgacag cttgcatcga ggaagcatac gttccaccga
     4321 ttcctagata atagacagcg tcaaattttt ccgcataaag tttatcgatg atttcttcta
     4381 cttgcggacg taaagctagt gcgcctttta ttgctttgat ttgttcttct tcattgaatt
     4441 tcagcatgat ttccacctct tataatttac ttttcaagac tgtatcaaag tcttcaaaaa
     4501 tctttttgtt caagtcaata cctttagcta ctgcggtttt ataagcgaat agttgaaacg
     4561 gaacgacaag cgctaataca gacagatatt catcacattc tattgctaat ccaagtgttt
     4621 tctcgtttct tgcttttttt gtggtaatgg tgagagtctc tccaacgtac cgactcatat
     4681 atctttgcaa cttttgcgaa cgttccttat ttacactttc cccttcaata aagaatagca
     4741 aatgcgaagc atctgcctcc aggtatgggc cgtgcatata agcttctaac tcaaatcctt
     4801 gtgacggaac gcggattgtt tcagtcaatt tcgtctctgc ttctttagct gttccccagt
     4861 ttggaccata tccaatggtg acgaaacgct gtgctaaacg aaataaactt tgatatccat
     4921 caaaaaattc ttccgttttt tgaataatcg ctggtaaatg gttgattatt ttgcgcaact
     4981 gttgcatgta gtcttgttct atttttttgc tgattttatt tttacttatt ccaattccta
     5041 tccctaagag tagtaactgt aaaacagtcg cagaatagcc ttttgtaacg aagcctactg
     5101 attctacccc ggcacctaaa tcaataattt gtgcagcttc taaagcaagt ggactttgca
     5161 cgtcatttgt gatagcaaca acaggtaacc cttgtttttg tatcattttt actgcttcaa
     5221 cagtagaagc acttttcccg ctttgtgaca cagcaatcac agtatcgatt gaaggagaaa
     5281 gttttccata attgaaaaaa taaaaaggtt cttgaatatc gacagtaaga tcaccataag
     5341 attccaatgc tggcttcgct gctaaacaag cgttataaga tgaacccgtt gctaagatca
     5401 gtaaattcac tgtttttttc atatgttctc ttgtcgaaaa atcattcttt ttcaagatcg
     5461 tacttagtac ttccttttct tcatagatat aatcttgcat cgtaaacatt ttctctgctc
     5521 cttttttaaa aaatccctat ccacgaacca aaaattccaa agaaagctag tccgatcaag
     5581 atcgttaatg gtttcacttc tttcttcaat agataaaaca ctatgccaaa tgctccaaga
     5641 cataagatat ttggaacgat atcatcaaaa atactttgaa cagtcacagc attttctcct
     5701 gaaccgattt ttaaaggaat attgatggtg atcatcgtgg cagtcattgc tccaacgacc
     5761 atcagtccaa taatagacgc gcctaatgtc aaactttcca tcataccgtt tgcctggatc
     5821 tttttcaaaa aacccgttcc taagcgatat ccccaacctg ttgctagata gcgaaacaga
     5881 atgtgcggaa tattgaaaat caaaatgaac aagatagggc ctaaaatatt tccttgcatg
     5941 gctaaagagg tacccacacc tgtggcaatc aatcgcaaag ttccccaaaa gaaagaatct
     6001 cctaaccctg caagaggtcc catcaaagat gttttgatac tatcgatcgc agtcacatca
     6061 aattcaggat catccttatt ttcttcctcc attgcagccg tgattcctaa aatcagtgta
     6121 acgatatgtg gggtcgtgtt aaaaaactct agatgccgtt tcaaagctgc tgccatttct
     6181 tctttcgact gatataactt tttcaaaatc ggaatcattg cataaacata agccatattc
     6241 atctgacgtt cataattcca agaaaactcc atttgaaaag aacgccaaaa tacgtgattt
     6301 agctcttttt tcgttatttt gctgtttttg atctcagaat tcttcatctt cttctacctc
     6361 ccctgcagtt gtttgaagtg gtagttgacg gacattcata atattgacta ccacaactgc
     6421 taaaatcgca ccaaaaatag ctactccggt cacaggaatc tctaagtaag aagctatagc
     6481 aaaacctaag aaaaaatatg gtgcaacttg tttgttgatc agcaaacgag caagcatcgc
     6541 aaaaccaaga gctggaatca accctgtagc tactgacaac cccttttgaa cgaagtcagg
     6601 aatcatattt aatagatttc cgatcgtatt gcttcccacc atataagaaa ccattaccac
     6661 aacaccaagc atcaaggaaa gaccgatacc ggcaagcaag tgcatatgct cgactccttt
     6721 ataatttcct tcttctgcat aattgtctgc tttatggttc ataattggaa tcaaaagccc
     6781 taagtaaata tttttcaata tcaatgtcaa cgtagcaatc ggcaaaccta gtaaaagcgc
     6841 ggtttctgta ccggctccag ctgtaatagc aaaagccgtc cctaaaatcc caccggtcac
     6901 aacatcaggt ggaatcgaag cacctactga gaacgatccg ataaatgcta actctaaagt
     6961 tgcgcccata atgatcccag tttttacatc acctaacacg attcctgtaa acagccccgt
     7021 cacaatcgga cgagaaagca acgatgtccc tagtgcatat tctgattgtg cgacaaatgc
     7081 aacaattcct aataagactg cttgtacaat cattgaggtt cttcctttct tacaaaactt
     7141 ctcttacatc cactttttta tctgctggta cttgacggat ttctacttct atttgtttgt
     7201 caattaatcg tttcaagcgt tcctcctctt ctggtaaaat atttaccgct tttccgatgc
     7261 tgcgagtacc aggttttgcc ttgatccctc ctaagttgat ggttttgatt tctggatacg
     7321 cttctgccag cttatatgca tcctcgatcg attccactac gatgaataac ttatatttat
     7381 ctgtcacccc gcttttcaac gcggcaatcg agtcatcaat tgatttgatc actagtttga
     7441 ctccttgtgg cttggctaat ttgatcgttg tctttcgtat ctcattcgta ggtacatcat
     7501 cattagcaat caaaatacag tctgcgccta ttgtttgtgt ccatgaaaaa gctacttgtc
     7561 cgtgtaataa ccgatggtcg actcttgtca atacaatcat ttttcttcct cctaaaattc
     7621 gtcttcttct attttctgat tgagccgctc gttacaaaac tggatcattt tttttgaatc
     7681 agttagcacc tgctggatca aaacagttgt ctctacttca gcgtccaacc gagatactag
     7741 ctcaatcaat agtggcaaat tcaatccggc aacgagatag acatgatccc gattgatata
     7801 ttgtaagaat tcgttgttga cacttccgcc aaaaagatcc gtcagaacaa tgatttcttt
     7861 atctttgtac ctcataaaaa gctgccttac tgtttccgct aaatcaaaat tctcatcagt
     7921 atagcaatcg aatacagtaa tagaatgatt tttcccatag atcagctcta atgaatctaa
     7981 tatccctgaa gcaaattttc catgggaagc taagataatt tctctatcca ctttttcatc
     8041 tcctcttttc acttacacat actaaaaaag caagttgcat gccaaaatta gtggcaacaa
     8101 cttgcttatg ttcaaaattc ttcttctatc gagagaaact ctgtatcttc actcaaaata
     8161 tcatagatat aggcaatctc agaattgggg atcatgacac tataatgctc ttcaatgaca
     8221 ctaaacgcag atttaatagc agataaccct tctttttgac actgcttcca tttctctaat
     8281 ccttgatagg tttcgattgg catttgtcgg atcaatcgtt caatgagaca gctcacatgg
     8341 acatacaacg ctaatttttt gggattgctg atgcttcgat tcaactgcac ctctaattct
     8401 ctcataaaat gatcgatttc tcccatcacc ttctctgtat ctagaatagt cacggaatcc
     8461 agtacttttt ccaatgaaaa attacgaatg atgcgctgat tgaatatttc cttctctgaa
     8521 gcattcatag tagtgtccaa ccattcagct agtacagaca tcttttcccc cgagatgagg
     8581 tcttctaatg aaagatagtc gatattggtg atatcaggat ctgtggtgcc aatcattcct
     8641 acgacttcgt atatggaaaa taatgggtca ctgtttttct tatcttttaa ttgctgatat
     8701 tcatatggga tgattttcaa ctgacaagcc gtaggtaagc ttttttctag cagttcactt
     8761 aagtggaccg ccgtaccgat tccagtgaag caagtagtaa tgagtgcttt gggtttgttt
     8821 tcctctggat agagcagtcg ccattctggc tggatctgtc gaacagtctc ttctgctatc
     8881 tcttctaatg atacattttt ttgcaggcat tcccctactg tgattgctaa aggtgtcgtc
     8941 acattattca aaatcaaaag cggtacggat aactgttttt taaaaaactg atggatttct
     9001 ttcagagatc ccatgtctac taatatgatc aatccatttg aaacatcgct gttttcgcaa
     9061 taatcaagga tttctgaagc aatctgctgt ggtgtgacat ctagtggcat atcaaatgat
     9121 tcaaatagat cctcttgtaa taaacgattt actacatttg ccatactgct agcagttgca
     9181 taaccgtgag cagcaatcat cgcttttggc agtcgttttt cttttgacca tttcgctttt
     9241 tttagataga tcgtcagtaa aatccgatcc atacttgtgt cttcaatatc taatttgatc
     9301 ttacttaatt ccagaattcg ttggacatag tgataactag atggataaga ctgagaaact
     9361 tgttgttcta attctttgat tagttggata atatcatgtt cttccggtaa ccatttgata
     9421 gtgcttcgct gatagagata gtaactcaag gcatagacac tgtttccatc gaatttgatt
     9481 tgataggcag tttccatttg tcgaaaggtc tctcggatag attgtgtcca ataaaccagc
     9541 agctcgtgtt tttcttgtct actggtctca aacagcaaaa aatcaaataa ttgaatcact
     9601 tcttgcttta attttccttc gcaagaatat agcagacagt tatttttctt gaaatcaatt
     9661 aggatttgct caaaactttt aacgattcgc tgctgttcgg gctgttgctt ctccattaat
     9721 tgctctaagg tctggtcttg cgtaatatag attccttgct gtgctgtatt tttagtaata
     9781 ggctgcgcaa gtagttcttt ttgcaaatga tggatcgtga catggatttc aggctgttca
     9841 cgctgttcag catatgcttt tgctgctgaa actttgacga tatttttcag ctctcctatg
     9901 tttccggata atttctgact tgttaaaagt gacaaagctg gtccactgac tgacaaaggg
     9961 ctccccattt ttctttgttc ttcgagaaaa aaagagtaaa tcagttctag ccgttcatta
    10021 cgactacgct gattaagtgc aggtagttcg attcggatag gtattcttcg aatgaaagtt
    10081 gttaagaaat gactagtaat ctcttcagtc gtagcaaaaa agagacgtgt tttgatccgg
    10141 cgtgacaaat taggttctcc catacgataa atcacacctt gatcaagata ggtgaacagt
    10201 ttttcctgac cttctggtgt taagcgatgt acctcgtcta aaaacaagat cccaccatct
    10261 gctgcttcaa aagccccttt tttattttct tctgcaccag tgtacgctcc cttaacatgt
    10321 ccaaacaaat tgctagtcaa aagttcagga ttatctgcat attgtgcaca attaaaagtg
    10381 atgaatggtg cagaatcttc tagcagatca tgaagcagac aatactgata gaccaaatta
    10441 actaaataac ttttacctgt gccactttcg cctgtaatca aaagtggtaa tccgccatct
    10501 ggatagtaaa gagctgtttt gatttgttcg atactttctt tcaaactggc gtcgtatccg
    10561 atcataaaag agaataagtc ttttggttgc gcgaaaaaag gctgttcctc tttgagatca
    10621 tcgatagaaa aataaaaatt cgtgcgtaac ttaaaaaatt gtttttcaaa agcttctttg
    10681 tggaaataaa tcactggtcg agaattgatt ttgactagta gttgctcatc atttaactgg
    10741 ttcaaataat gactgactgt gtttcttttt accccaaaat gatcagctaa cctttgtgct
    10801 gtaaagacct cgttcatatg ttctaaatcg aaaaaggctg tctggttctc taagtaacat
    10861 aaaatttcct tcttcaacaa cttattcacc cctcctccat catagaaaac ggattcattt
    10921 tgtcaataaa atctttaata aagaattgaa aaatatatct tattatagac agcagcctac
    10981 ttatccctta taatttttat tctaaaaaac gtacagttcc ttccagtttt tatactggaa
    11041 tttcaacata tgaaagaata aggaggctat gactattcaa tgaaatcgga taaatataaa
    11101 aacaggaaac atacttcctg gaaaactttt cgagaaatca tcggatctta tctcgctgta
    11161 gttgtatttt tgcctttgct ttatatggct atgacagaac aaactcgttt tattggcgta
    11221 gatacgcaat atatagccga aacaggattt ttattcatct ttatcttgat ttttgcgttc
    11281 tataaatcct tccatacaaa ataagattca cagatttcaa ctttcctgtc tgtttttagc
    11341 tattacaaat ccttatatgt ggtataacaa caatttattg gcataatgag gaaaaacgag
    11401 ttatgatgct ttcagagagt aaagaggtac tgttgtcacc atccttttta ttgattacgt
    11461 acaccactct tagttttttc ccattgatta cgaaaacaag gtagaaacat tgatgttcct
    11521 accttgtttt ctttttttat tttcagatca aaatctcaag tagcttttga cttgatgtcg
    11581 ggaggttgtc ttcgttccat caaacctgat agaatctgcg ccaaaccata gttttctcca
    11641 ctattattta ttcctctaag atagtaccca caataaacga ataaatattt ttctcatttt
    11701 catactcctt ctttgaaaat caattagatt tctctcaacc cactttttta caaaaattac
    11761 gtagcaaaaa agattgcaag aacaaattaa aaaagaagta aatagagttt catctataaa
    11821 catttacttg atatattttt tatgcagaat tcatttatag aaaaattaag taaaatagac
    11881 gttcctatga atgaagagac tggcatgttt tctcattttc ttttttttaa aaaaaatagt
    11941 ctattcaatt tctaaagctt cttcataata aaactttcat ttatgaaaag gcttgttctc
    12001 ttttcattct aaatttccta agctacaatg tggtagtact tgttttttca attgtggaca
    12061 aataaataat aggaggaatc atatgattca gttattacgt tatgcaaaag actatcgtaa
    12121 gcaaatcatt ttgggcccgt tcttcaaatt tcttgaagct gtcttcgaac ttgtcctgcc
    12181 tttaatgatg gcttccttga tcgataatgg actaaaaatg aatgaccgag gaaaaatcat
    12241 tgaaatgggc ttatggatgg tcgctatgtc tgtgattggt ctgatttgtg ctatcatttg
    12301 ccaatactat gcttcagtag cttcacaagg atttggtaca gagttacgta atcagctaat
    12361 caaaaaaatc aatactttct ctcacaacga gttgaatcat tttggaacag ataccttgat
    12421 cacccgaatg acaaacgaca tcaatcagct tcagctagct ttagcaatgg tcattcgttt
    12481 attgatcaga gcaccgtttt taagtatcgg gtcagtggtc atggcttttg tgatcgactg
    12541 ggaagtaggt ctgtttttct tagcattatt gcctattttc tcaatcattt tattttttat
    12601 catcaaaaaa accgttcctc tctaccaaaa agtgcaagaa aaactagatc agctcaatga
    12661 acacgttagt cagaatctta gcggtgttcg tgtcatccgc gcatttgcgc agacaaaaaa
    12721 agaaacagag aagttcgata aaagcacaaa tgatctagcg aacaattatt tacgtgtttc
    12781 caatatctct gccctattat cacctgctac aaccttgatc atgaatgtcg ggatcatctg
    12841 tttgctgaca gttggcggga tcaaagtaaa tattggtagc ttgcaacaag gacaagttct
    12901 tgctttgatc aattatatga atcaaatgct tttggcactg atcgttgttt caaacctcgt
    12961 cgtgatcttc acacgtgccg aagcttcagg aaatcgggtg aaagaggtgc tagacacaga
    13021 aaattcgatt cttgaagctg aggtcagtac tactcccgat ttttcttctg aagcaattat
    13081 cgcttttgac catgttgact tccgttatac tcctgattct ggtctttctc tccaaaatat
    13141 tacatttcag ctgaagcgaa atacagtcct tggaatcatt ggtccaaccg gaagcgggaa
    13201 aacgactttg acacaattga ttccgcgttt ctatgatgtc agttccggta cagttataat
    13261 ggacggaatg gatgttcgtt cttggcagct ggatcatctc cgtcagcaaa ttgctcttgt
    13321 tccccaaact tctgtactat ttaccgggac tattcgtgaa aaccttcaat ggggtaagaa
    13381 agacgccaca gaaaaagaat gttgggaagc tctgaaaatc gctcaggcag aagaatttgt
    13441 ccgtcagctt ccaaatggtt tagatacaaa agtcatggaa aatgggaaaa atttctctgg
    13501 cggacaaaaa cagcgactga cgattgcaag agctttgatt gccaagccag cattactgat
    13561 tttagatgac tctttaagtg cgttggatta tcaaacagac ttggatttgc gtaaggcgct
    13621 acagacttat cttggcagta cagtgattat tatctcccaa cgtgtccgct ctattcaaga
    13681 agcacatcat atcttagtga tggatcaagg taagattgct gctcagggta cccatgaaga
    13741 attacttgct caatcagcag aatatcagga aatcgtcgca tcacaggagg aagaatagtt
    13801 atgaaagtaa aaaaattatc cttgcagacg ctccgccgat tcattcctta ccttactgcc
    13861 tatgcaaaag acatttggct agctgtgata ctaggaatca tcaatgggat tgcaacagtt
    13921 gtgatgacgt tacaaattgg tcaaagtatc gatcaaatga ttggagaagg acaggtccgc
    13981 ttcaatcgac tgttccattt acttattcta ttcggtggta tcgttttaat caacgtagtc
    14041 agtcaatggc tcatccaagt gttaagcaat cggttagctt acttatccgt tgcaaaatta
    14101 agaaaagatg catttgctca tctgaatcga ttgccacttc gttattatga tcaaaactcc
    14161 cacggaaata tcgtcagtcg atttacaaat gatatggaca atatttccat tgcaatttct
    14221 gcggccttca atcaattatt ttctggagtg gcaatcatca ttctttcgtt tatttttatg
    14281 atgcgtctaa gtccacttct gacattggtc gttctttgtt ctacaccaat tattttcctt
    14341 gtgagttgga tcgttgctcg tgcttcacaa acaaatttcg atagacagca aaggattatt
    14401 ggggatatct cgggctttgt gaccgaacga atcggtaacc agaaaatcgt gaaagctttt
    14461 cagcaagaag aagctagtca acagcaattt gaactgctaa atgctgatct atatgaaaaa
    14521 ggacaacggg cacaattttc ttcttccttg acaaacccgt cttctcgttt tgtagatcac
    14581 cttgcttatt tatcgattgg tttagttggc ggcctattgg cattgaagag tggttcgtca
    14641 atcactgttg gtgtgatttc cagctttacg atctactcaa gtcagttttc taaaccattt
    14701 atcgaacttt ctggaatcac gacacaactg caaaccgctt tttctggtct agaacgagca
    14761 tttgatttga tcgatcagcc agaagaaaag cctgatttgc cagatgccta tgccttggaa
    14821 cccaaaaata tcaaaggaaa agtctctttt gatcatgttt ctttttctta tgaccccaag
    14881 cagcgtttga tcgaagattt caattttact gcttatccag gtcaaaccat tgctatagta
    14941 ggaaaaacag gggccggaaa atccacactt gttaatttgc tgatgcgttt ttatgacaca
    15001 acaaaaggaa caattacagt cgatgaccat gacatccgct ctatccaacg tgatacatta
    15061 cgtaaaagtt ttggaatggt gttacaagat acttggctat ttgacagtac attgcgagaa
    15121 aatcttcgtt ttgggaatcc ggaagcaagt gacgcagaaa tcaatgatgc cctaaaagcc
    15181 tcttatatgt atgaatttgt agaacgtttg ccggaaaaat tagatactaa gattggcggt
    15241 caagggttaa agatatcaga tggacaacgc caactgttga cgattgctcg aacgatgatc
    15301 agtaatcccc ctatgttgat cttagatgaa gctaccagct cagtcgatac attgacagag
    15361 aaaaaaatcc aacaagcctt cttagcaatg atggaaggaa aaaccagttt tgtgattgcc
    15421 catcgcctgt ccacaatcaa aaatgcggac cagatattag tgatggatca gggagctatt
    15481 gtcgaaatgg gcacccatga tgagctgctt caaaaagatg gttattaccg acggctctat
    15541 caagcacaat tcacagcaca ataaaaacaa gcagtcttct gggatgggaa gactgcttgt
    15601 ttttt
//
LOCUS       DASHRQ010000063        15401 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.067, whole genome shotgun sequence.
ACCESSION   DASHRQ010000063 DASHRQ010000000
VERSION     DASHRQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.067"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..164)
                     /locus_tag="U2M88_002183"
     CDS             complement(<1..164)
                     /locus_tag="U2M88_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEM8374538.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTG"
     gene            194..604
                     /locus_tag="U2M88_002184"
     CDS             194..604
                     /locus_tag="U2M88_002184"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374539.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            685..1305
                     /locus_tag="U2M88_002185"
     CDS             685..1305
                     /locus_tag="U2M88_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374540.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            2006..2290
                     /locus_tag="U2M88_002186"
     CDS             2006..2290
                     /locus_tag="U2M88_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374541.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            2293..3210
                     /locus_tag="U2M88_002187"
     CDS             2293..3210
                     /locus_tag="U2M88_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8374542.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            3203..3985
                     /locus_tag="U2M88_002188"
     CDS             3203..3985
                     /locus_tag="U2M88_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8374543.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            3986..4666
                     /locus_tag="U2M88_002189"
     CDS             3986..4666
                     /locus_tag="U2M88_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8374544.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            4659..5555
                     /locus_tag="U2M88_002190"
     CDS             4659..5555
                     /locus_tag="U2M88_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEM8374545.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            5750..7399
                     /locus_tag="U2M88_002191"
     CDS             5750..7399
                     /locus_tag="U2M88_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEM8374546.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            7993..8928
                     /locus_tag="U2M88_002192"
     CDS             7993..8928
                     /locus_tag="U2M88_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8374547.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            8921..9691
                     /locus_tag="U2M88_002193"
     CDS             8921..9691
                     /locus_tag="U2M88_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8374548.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            10107..11090
                     /locus_tag="U2M88_002194"
     CDS             10107..11090
                     /locus_tag="U2M88_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEM8374549.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            11537..12571
                     /locus_tag="U2M88_002195"
     CDS             11537..12571
                     /locus_tag="U2M88_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEM8374550.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTRAGS
                     NQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKILV
                     INGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELGI
                     DQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMFA
                     KTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHMLG
                     KVVVSGPFNG"
     gene            complement(12660..12983)
                     /locus_tag="U2M88_002196"
     CDS             complement(12660..12983)
                     /locus_tag="U2M88_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEM8374551.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(12970..13677)
                     /locus_tag="U2M88_002197"
     CDS             complement(12970..13677)
                     /locus_tag="U2M88_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEM8374552.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            13763..14104
                     /locus_tag="U2M88_002198"
     CDS             13763..14104
                     /locus_tag="U2M88_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HEM8374553.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(14275..15123)
                     /locus_tag="U2M88_002199"
     CDS             complement(14275..15123)
                     /locus_tag="U2M88_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374554.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
BASE COUNT         4923 a         2630 c         3246 g         4602 t
ORIGIN      
        1 cccgtgttaa aaccaatgcg atcgtacttt tcgtaatcga ggaaagccgt taactccgtt
       61 ttgagaagcg tattgacagc tttttccaaa tggacacgga ataattcatt caaatcgcct
      121 ttgttcgcta gagtctttag aatttctgta gtaaaatcgt tcatatggaa gtcctctttt
      181 ctgtgaatgt gttgtggtaa ctctattcta cagaagggac ttcctttttt ctatttacac
      241 aaaatatttt acactctcgt caaatataaa aactagtacg aaattaattt gtaaattatt
      301 taagcttgat aaaataagca atatcgatga tgtaaagtcc aagatattaa caagaaaagt
      361 aagcgaacca gtaaagctaa cggcacaaga taaaaaaaat ggaattgttc tttatggtga
      421 tgatgcaaag tataactata attgggatac ctcctcagaa gctgaggtat taagtattcc
      481 tactaccgat acttcagggg aaaaagtcac ctttaaaata aattttataa cggcaatttc
      541 taagttggag tatgggaagc aaaaaaagag agataacaat gatcaatatc tacctaaaaa
      601 ataacgaatt aatgatgcat atgttaaagt tgtttttttg aattaaacaa ctcaatttat
      661 tcagttatat gttcatcgca gcaagtgaac attaatcgag ttataaaatt aataggagct
      721 aaaaatataa ataaagttcg atcagaattt atttttccag aagatttttt taactggttt
      781 ttttatcaat tcagtgaatg caagcgactc ttaggtgaga attattactt aaaaggaatc
      841 ggcggattta tggggaatgt ggctgatgaa caaaatatta taaaaggaat atctgaacaa
      901 acatcagagc ttacagtaac taaagcattt ataagtaatg gtgaaagttt gaaaaatgta
      961 actgcgcgaa ttagaaaaga aaatgaagta gacattgttt ttggaattga ccatgaatgt
     1021 aattcagtga tatatgtaac acaatcagca aatttaattc tttttgagaa tggttgcaag
     1081 gacgatttta tggtggtcta tttgtacgat atactaattc caaaacttcg ttccttatat
     1141 atgtcttctg aaagtatttt tgtgagtgaa caaaagggat ctttctcgaa aaaaataggt
     1201 attgaagtaa ttgaatcgat tatgtgtcat aatcatatta cgctagaaga attagaaatt
     1261 ttgatagggc aaaaaaataa taacacgaaa atacaagttt cttagaaatc attttttatt
     1321 gagatatcta gataaatgat ttattcttca atcaaccaac tcgccaagct gaggtaatgt
     1381 tttgaggtaa tgataacata caacttttta taccctagta aaactctata aacaaagact
     1441 tattaatttc gcatgaaata cattcctgta aaatgcaata aaatagagtt ttgtttaacc
     1501 aggtcttaag aaagcccgta aagcttcaca gttctcaaaa cgttaattgc gattatcgtt
     1561 cgagttcacg agttacgaga atcgtactgg gcgaagcaat gctgagcgca gttaccttaa
     1621 tcctaacttt tctggcactt tccacaattt ggaaagtgcc ttttttgtgt ttttagctat
     1681 gttaataggt aggattcttg gtctataata tcattcgatc tataaacaaa cgggaaaaag
     1741 ataaagtatc atcaaacaat attatttatc cgcttctgag gttgtgacag aagtggtcag
     1801 cttcaagaaa taagaaggaa tttacaaaaa ttgcttctca tgtttgataa atattgtgac
     1861 ttgtgaccga ggagttggca ggagaacacc gtttattcgg aagaagggat tgtgaaaagt
     1921 cttttgtcat aacctcttat ttggtagcta attgtaaatc ccaaacggca tgtattattt
     1981 ttcataagtc cgtttttaag gattaatgtt ttcttttgct ttttatatgg tctggcttca
     2041 gcgagaagtt ccaacgcata tacaaggacg tctgatgtca acgataacaa cgattaccat
     2101 ggtggccagt ccacttggat tagcactgtt tggtgcgctc tttgattggc agacaactca
     2161 accacgtctg cgagacagtc tgatttttct tgaaattcaa ttggaaaatt tacactttct
     2221 ttatacattt ctggctgctt tctttatacc cgtcaactat acttcactta tcaataagaa
     2281 agaaggataa gtatggattt tggaaatgaa acggtcttga aagcaactgg tataaccaaa
     2341 aaatacggtg ctgccaaagc attagataaa gtatcgatcg aaatcaaacg tggaatgatc
     2401 tatggtttga ttggggagaa tggagcgggt aagtctacct ttatgagaac tattatgggt
     2461 ttgatctcga ttgatgaagg ttcgatcgaa ttatttggaa cgacggattt gcaggcagca
     2521 agacgacgta tgggtcaatc gattgaaacc cctgctcttt atccagaatt aactgcaaga
     2581 gataacctac gtatccaagc agcgaacggc ggtgtgtcgg atagagaaat cgaagacctg
     2641 ctgaaaatga tgcgattgga aaatacaggg aaaaagaaag caaagaattt ctctttaggt
     2701 atgcgccagc gtttagctat tgcaaatgca ctgatcacga atccagaatt tttgattttg
     2761 gatgaaccga cgaatgggat ggatccagca gggatggcag aaatgcgaga aatcatccaa
     2821 cgtttggtaa aagaacgggg catcaccgtc ttgctttcta gccacttgct agatgaattg
     2881 tcacagatcg ccactcacta cggtattttg catgaaggac acttgatcaa agaattatcg
     2941 aaagaagaat tggcacaaga atctcgtcaa tttataaaaa ttgacacttc ggcgacggaa
     3001 caagcagtaa cggttctcga ctctcttggt tatagggatt attttgtcca atctagccgt
     3061 gttatccagc tttttgaagg aattgaccaa gtagcagcca tcaatcaagc attggtagag
     3121 gcaaaagttc ctgtggatgg tatccaccta gttgggcaaa aactggaaga ttatttctta
     3181 caattaactg gaggcaatcc acatgtttaa tctattaact gctgaaaaaa taaaagtatg
     3241 gaagagccgt aaaatgtggg tctcactagg actgatggtc gtacttccgc ttttttattc
     3301 ttggaacgaa tggtatatgc atacaaaata cggcaatgaa ttaaaccaag caacggatac
     3361 agtcatcaac ggtgcaacag ggattttaat ggtggaaaaa atggctgcat ggattcttct
     3421 ggctttcgca gcatttgcgt gcttttatat tggggaagaa tttcaaaacg gtacgattcg
     3481 caatacgtta tctttaggac ggaatcgaat gacttattat gtgtcgaaac ttctcgttac
     3541 gcttttgatc acaacacttg gcgtggtttt gattacgggg ctagccatga ttgcttacac
     3601 acttgctttc ggatttggtg aagttgaggg aatcaaaaac tatggaaatt atgttctaaa
     3661 agtatttcct gtattgcttc tgctgatatt agcgacatta tctgttccag tggctttgac
     3721 attcatcact agaagtacca gtgtcagttt gcttctttct tttctttata tcatgggaac
     3781 ggccttcgtt cccggtgttt tcgcaaaaat caaaggcttg gaatttctaa cggagtggtt
     3841 tacggaaacg tggctcatgt atacggactt tgcgcaacaa gcaacttatt cgcaagcacc
     3901 gaaaatgatt ctggtcagtc tagtaacaat cgtggtatca tcagctctag gaatgtttat
     3961 tttccaaaaa tctgacatta agtaggtgtt cctgctggaa actattttga ttatcgaaga
     4021 cgatgaagct gttcattctt tactggagga ggttcttgaa cagagataca aacttcttga
     4081 tgcttattca ggtacggaag gaaaattatt gttaagcaca tatcctgtcg atttgatcct
     4141 tctagatctc atgcttccag ggctttcagg tgaagcattg ttagcagaaa tccgacagac
     4201 atcgaatgtt ccgatcatcg ttctttcagc aaaaagcgat caaagagata aagtttcctt
     4261 gttggcagca ggagcggatg actatgtgac gaaaccattt gatatcgaag aactgttgtt
     4321 acgaatcggc atccaacttc gtcatcagac ttctgtccaa gtaaaagatc tgtcgcaacg
     4381 catctattac aaggaaatct tactggacaa agagtcgagg gatgtaaatg tgaatggtgt
     4441 ggctgtccat ttgacaggaa gagaattcga tctgctgaag ctttttttag aacatccgaa
     4501 aaaagtattt tcgcgagcaa atctttatga aacagtttgg caagaaccat attttgatag
     4561 tgagaaaaca gtgaatatgc atatttccaa tcttcgaggg aaactagcaa aggcacatgc
     4621 tgtgtatatt catacggtgt ggggagtggg gtttagattt gattaagtgg ctgttgcttc
     4681 tcgttgttat ctttatggga agttttattt tcttcctgca aagggaaatc aagagattaa
     4741 ctaagaaaat cgccactctt tctgaacgac aagaatatgg tggtcgtctt tcagtcgaat
     4801 ttcgagaaaa gaatctaatg gacctagtag atgcactgaa tacatgggtg gatgaaaatg
     4861 aacaaaaagg acaagcattt caagaaatgg aagaaaacat cagactatcg atcgtcggcc
     4921 tttctcatga tctcagaact cctttgactg ctattaacgg ttatgtacaa ttactggaac
     4981 aggtagagga tacggagaaa cgggagaagt atttaacgat catcaaacaa tctgttgcaa
     5041 gattactcag tatgacggat cagttttatg atcttgctcg aatagagacg aagcaaaaag
     5101 aaatggatct ccatacgctt tctttttcta aagaggtgga agaagtattt ttctcttttt
     5161 atgaacaatt ttctgagaaa aatatcgagg tagcgttcgc tgaacactcg ataaatacaa
     5221 ctgtattagc agatccgctc atgttgctgc gagtgctgca aaatatcata cagaatttat
     5281 tgcgttatgc agatggacaa gcaaaggtga cattttcaaa agaggattca ttcattcgat
     5341 taacagtggc caatcctatc aaaccaggag cgcaaacctc tgttgaaaaa gtcttccagc
     5401 gtttttatac cgaaaatacc agccgcacaa atacggaatc tagcggaatc gggctttacc
     5461 tgtcaaaaaa attggtggaa ggaatgggag gagagatgac cgctagatta gacgggggca
     5521 tcttctccat ctccgtcaag ttgcggaggg tttaattggc tctaatttac ctgaagtgaa
     5581 tgacaaaaaa ttgtatctcg ttttaaagac atcttctttt ttggaaggtg tttttatttt
     5641 ggtgtgattg atacgaatta ggtaacgaag acagttattt ttgtaccccc ttttcaaata
     5701 gcgcaacagt gctatactaa aactaacaaa tcaaggaacg gagagggata tgggccaaag
     5761 agaatttatc gttttaatta ttattgcagt tgttatttta ttagttgttt tagacatttt
     5821 ttcacgtttg aaagtaaagg aaacagttcg tagtaattgg ggaaaaattc cttatcaacc
     5881 gagatttgat aaagaagaaa gtttgaagga tgcgtggctc acggagaaaa aattccgttc
     5941 atgggatagt gaaatcgacg atcttacttg gtatgacttg gatatgttcg aagtatttga
     6001 agggatcaac agtacctatt ctagtgtagg ttcagaagcg ttgtatcagc gtttacgtag
     6061 ctttgatttt ggagaagatt atcagttaga aaaactaatt gctttttatc aggaaaatcc
     6121 tcaactgaga gaaagaatcc aatatcaatt cgctcgttta ggaaaaaaag atcataattt
     6181 tgcaaaacaa tatctagcgg atggaaaaag taaaaagatt ggacgcgtag gtttatttat
     6241 ttttcttggc ttgcttccgc tggtgggtgt aggtttgctt cttttagggc aagtactagg
     6301 aatctatcta gcaatcggtg gcttagtatt caatacggtc tattatctag tgaagaaaca
     6361 aacactagaa acagagctga atagcatgag ttatttggtt tcaacgattt cagccgccaa
     6421 tcagttagca aaaatgacga caccacttca aaaagagctg aaacagcaaa ctaaaccatt
     6481 aagagacatc cctaaattag gtttttcatt tcgaatgaag agcgggagtg aagcagaatt
     6541 gatttttgaa tatgtcaaca tcatgttcat gctgccattt atctcctatc atttcgttct
     6601 cactaaatta gaaaagcatt caactgaagc catcaagctg tgggaattgc tgggagaatt
     6661 ggaagttgca gcagccgttt tgaattatcg cacctatatg ccgatcactt gccgaccaga
     6721 gtttactgag ggcggtgtga cagcgaaaga tatctatcat ccattgttaa aagaagcggt
     6781 agttaatcca gtcaactggc agaagaatac attagtcacg ggttccaatg catcaggaaa
     6841 atctacgtat gtcaaaagta tagccatcag ctgtattcta gcacagacga tccaaacagc
     6901 gattgcagaa cagtttaccc tgcaacctgg acatgtgttg acttccatgg caatcgaaga
     6961 tgatttattt gaaggcgaca gttattttgt ttcagaaatc aaatcgatca aacgtttgct
     7021 ggaccaagtg gcttccaaag aacgctgcta ctgcttcgta gatgagattt taaaaggaac
     7081 aaatacgatc gaacggattg cttcttccgc ttcagtcgtt cggtggcttg cggagtatcc
     7141 atctttagca tttgtcgcca cacatgatat cgaattgaca gagattttaa agaattattg
     7201 tgagaatgtc cactttgaag aacaagtaac agaaggaaaa ggaatcagtt ttgatttcaa
     7261 attaaaagaa ggaccttcaa ggacccgaaa tgcgattgcc ttgctgaaag ttttgaatta
     7321 tcctaaatcc atagttgaac aggcccaaaa agaaagtttg ttgttcgatg aacagcgtca
     7381 gtggtatccg tttgattaac tttatcaaac gaagcatgat gcgtgggtcc aaaaagacga
     7441 ttatcaaaag aagtacattg atgccaaaaa agtggcagaa ttcaatggga acgagtctgc
     7501 tgtccagatg atcttggaag atttagcaaa agacgataca aaataaacca cgctgtcttc
     7561 cttctagcag cttttatcga aaaaacttag catcagcgac gagacgcacg ttccatggca
     7621 aagttctccg taaaacaaac agcgaaaaca gaaattgaaa ataactattt ccattttatt
     7681 tatttcggtt aaaataaaag aaaaaagctt tcgataaaag gacggaggaa agcttaacat
     7741 gaataaaaaa gacaagaacc tcagttataa ggatcttcta tatgtcatcg tagtagttgc
     7801 tgtcggtact tttatgattt taaaagttcc agagttgttt agatagttat tctatcgaag
     7861 atgatcatga actagaaaac acatctcctc tttgttagca ggtgtgtttt tgtgtggaag
     7921 ccaagttgtt ttttcatctg ctgtattcca tgatacaatt aagaatcatg tctataagga
     7981 gataggaaat aaatggcaaa tgcgcttgaa ataaataatt tgaaaaagat ctatggaagt
     8041 ggggtcgaag cattgcgggg aatcgatctt tcggtgaaag aaggagattt ctatgcgcta
     8101 ttagggccaa atggagctgg gaagtctaca acgatcggga ttattacttc tttagtcaat
     8161 aaaacatctg gaaaagtgaa tgtcttcggt tatgatctgg acaaggatct agttcgagca
     8221 aagcaacaga ttggattggt tccacaagag ttcaatttta acccttttga aacggtccag
     8281 caaatcgtcg tgaatcaagc aggctactac ggtgtctctc gcaaagaagc gatgaaacgc
     8341 agcgaaaaat atttgaagca gtccaatctt tgggaaaaga gaaatgtccg tgcacgtatg
     8401 ctttccgggg gaatgaagcg aagactgatg attgctaggg cattgatgca tgaaccacgt
     8461 ttgctgatct tggatgaacc aacagcagga gttgacatcg agttgagacg cgagatgtgg
     8521 gagtttctac gagaattgaa cgaaaatggt acaacgatca ttttgacgac gcattatcta
     8581 gaagaagctg aaatgctttg tcgcaacatc ggaatcatcc aatcgggtga actgatcgag
     8641 aatacaagta tgaaagagct tctgtcaaaa ctgcaatttg aaacatttat ctttgattta
     8701 gcgccatatg ataccaaacc tgtgattgaa ggatatgctt gtacatttga agatgagcag
     8761 acaatcgctg tcgaagttga acgaaatcaa ggggtgaacg gaatcttcga tcagcttaca
     8821 agacaaggaa tcaaagttct ttctatgagg aataagtcga atcgtttaga agaactgttc
     8881 ttgaaaatca cagaagaaaa taatcatacg gaggagaaaa atgtttaatc tttattttac
     8941 tgcattaaaa agtttagcta tcaaggaaac gaaccgctat ttgcgtatat gggtccaaac
     9001 actggttccg ccagtgatca cgacatcctt gtattttgtt attttcggaa aaatgatcgg
     9061 tgggcggatc ggtgacatgg gtggtttttc atatatggaa ttcatcgttc ctggtttgat
     9121 tatgatgtct gctatcacta gttcgtatgc caatgtttct tcgtccttct tctctcaaaa
     9181 atttcagaaa aatatcgaag aaatcttggt agcacctgtt cctacgcatg tgattatttg
     9241 gggctttgtg attggtggtg tgggtcgcag tattctcgtc ggctctctcg taacgatcat
     9301 ttccttgttc tttgtaccac ttcatgtgta ttcatggttc atggtcatca ttacattatt
     9361 gatgacggca atcttgtttt cattagcagg actgctcaac gggatttttg ctcagtctta
     9421 tgatgatgta tcgatcgtgc ctacatttgt cttgcagccg ttaacttatc taggtggcgt
     9481 tttctatgcc atctctatgt taccgccctt ttggcaagct gtttcaaaag tcaatccgat
     9541 cgtttatatg atctccggtt tccgttacgg attccttgga acgatcgatg tgccagtagc
     9601 aacatcgatg atcgtacttg tgttattcat cgttgttttg tacgccgtta cttggtattt
     9661 gatcaataaa ggaagaggat tgagaagtta gaaaagaggt tgtgaaatca gcgttttgac
     9721 ctgagtatta gagaaacaaa cgggaaaata gttcctcata ttttgttgtt ttttgagcga
     9781 ataccgaggg tcagctgatt gaacaccgtt tagtggagag gttgtgaaaa agctgttctg
     9841 catcaagtaa taagaacagc caaacaaaaa tagctcctcc tatttttcgt ttggttggac
     9901 ttattaccgc agatgcaagc ttttgaacac catttattcc ggaaaatgga ctgtgataag
     9961 atttttgtca cagtcttttt tttgctcaat cagaaacatc ttttaagctg ttcactttgc
    10021 ttcttttttt gtttcctagt ataatgacaa tgtttttgag aaagattgcg acttcactgc
    10081 ttgatagaat ggagagtaac aaataaatga ctactaattt ttggaaagag ctgccaaagc
    10141 ctttttttgt tttagcacca atggaagatg taacagatgt tgtatttcgc catgtcgtaa
    10201 agcatgcagc agcaccggat gtcttcttta ctgagtttac aaactcagac agtttctgtc
    10261 atccagatgg aaaagacagt gtacgaggaa gattaacatt tacagaagat gaacagccga
    10321 tggttgccca tatttgggga gacaagccag aattttttag agaaatgagc attgctatgg
    10381 cagaaatggg cttccaagga atcgatatca acatgggctg cccagtccct aatgtagcag
    10441 aacgtggaaa aggcagtggc ttgatccttc gtccagaagt agcggcacaa ttgattgaag
    10501 cagctaaagc tggcggtttg cctgtcagcg tcaagacgcg tatcggctat acggaaatgt
    10561 ctgagatgga agaatggatc agtcacctac ttcatcaaga tatagccaat ctttctgtcc
    10621 atttgcgtac gcgtaaagag atgagtaaag tagatgcaca ttgggaagtc attcctaaaa
    10681 tcattgcttt acgtgaccaa atcgctccac aaacactaat cacgatcaat ggagatatcc
    10741 ccgatcgcca gacaggagaa gaattggccg agaaatatgg cgttgatggc atcatgattg
    10801 gccgagggat tttcaagaat ccttatgcat ttgaaaaaga cccgagggaa catacagaaa
    10861 aagaactgat cggactactt cgtctgcaac tagatctgca agatcattat tcagaaatca
    10921 tcccgcgttc catcacagga ctacatcgtt tctttaaaat ctatatcaaa ggatttccag
    10981 gagcaaatga cttgcgagtt cagttgatga atacgaaatc tactgatgaa gtacgagaga
    11041 tcttagagac ctttgaaaaa gaacatccaa cgcttttcag tgaacaatga tttcttgctt
    11101 gaacaccgtg aattagaggt cgtgaaaaag ctgtcctgta tcaagtaata agaacagcca
    11161 aacaaaaata gctcttcata ttttgtcgtt ttttgagcta ataccgaggg tcagttgatt
    11221 gaacaccgtt tatatcagag gttgtgagcc tgccgtttaa ctctgagtca caaggagcaa
    11281 tttcaaaaaa cagcttctca tgtttgatga atattttgac ttgtgaccga agagttggta
    11341 ggagaacacc gtttataaga ggttgtgaca aaagtcttgt cgcaacctct tttttaacca
    11401 aaaaagtgca aatagtttac tttaagtaag agaataatta tgatgaataa aaaaggtgga
    11461 acaaatagca tactatatat attgaaatga acactgtatg gatgctccaa cttgatgaag
    11521 ctaaggagga attcgtatgt cagaaaagga attaatgaag gtagtcggtt tttatgaagg
    11581 gttgccgatc gatgatccaa aaagctttat tgatgaaaaa gaacagatac ctgtacccag
    11641 ttcacgggat cttctggtaa aagtcaacgc agtatctgtt aatcctgtcg atacgaaact
    11701 tcgccaagat aacgggataa gaaatgcttt acgtatctta ggttttgatg gagtcgggaa
    11761 agttgttgct gtaggtgacg aagtgcaaaa attttctgtc ggcgatcgcg ttttttacgc
    11821 aggtacaaca agagcaggaa gcaatcaaga ataccaacta gttgacgaac gtattgtcgc
    11881 tcttgcacca aaaaatcttt cagatgaaga agctgcagcc ttgccgttga cctcattgac
    11941 cgcttatgaa ttgttatttg aaaaatttgg tctaactcca gaagaaaatg ccaatcgcgg
    12001 gaaaaaaatc ttagtgatca acggatccgg gggcgtcggt tcgattttga atcaactagc
    12061 gcattgggct ggcctagaag tctacgcgac tgcaagtccg aagaattttg aatggctgaa
    12121 aaaaacaggt gtagactatc caatcgatta tcatcacgac ttaagtaaaa gttttcgtga
    12181 attaggaatc gatcaagtag agtatgtcgc tgtattattc gatattaccc gctactttga
    12241 tcaaatcaag caccttgtcc gaccatttgg acatgtggga acgatcgtag ggatcaaaga
    12301 gccgttagat ataggtgttt ggaaaaacca atctatcagc ttcgactggg agtatatgtt
    12361 cgctaaaaca gactataact atgaaatcga aacgcaagga gaagctttag cgcacattgc
    12421 tgctttagca aatgaaggga ttatccgcag tacattgagc aaggtctatt cagatgggat
    12481 gaatgcgaag aacttgaaac aagcaaccaa agacgtagaa acaggacata tgttaggaaa
    12541 agtcgttgtt agtggtccgt tcaacggata agcaagaata cgccgatttt tttcttattt
    12601 gactttgata aaaacaagag cgcatgacag taatttgcca taagctcttg tttttttatt
    12661 tataataagc gaatcaacga aagggaaaga attcctgcca agacgatcac caataagctt
    12721 tttgaaagaa tagcagctaa aagtgtagga actgaagcca aagcattttc gagattgatc
    12781 tgcggcagat gtccgatatt ttgggtaaat agactgctga accataaagc agacataatg
    12841 acgatcggga caaaacttaa atactcaagc aacggttgcg gtaaatcgaa ttttttcagt
    12901 aagacaaatg gtaggaccct agaaagccaa gttgctaaag tacaacctaa aatagtcagc
    12961 aatgtaaact caaaagaagg catgtttgat ccacaccccc aatccgcacc caatcagtgt
    13021 tacaactaca atcactaagt tgcttgggat aaaaatcagt ccgacatata ccagaatcaa
    13081 agtgagacca atcaggatca actgtaatcg tttagaggtg tttctatcag aaatgacttg
    13141 aagataaagt aacccgataa acatagcgac gatggcgaaa tccaaaccaa actttttcgg
    13201 atcagtaata aaattcccta aaagtgcgcc aacaagagaa gaacccaccc acgtcaaata
    13261 agaaatccaa ttagctgtat tcatccaacg aaagtttaac ttttgtccag tatagttcaa
    13321 tttattcatt cccagtgcga aactttcatc tgtcagcaat gtgccaatca gcagattttg
    13381 aagcagccga tttttcttga aataaggagc aatcgtcata ctcatcagaa tcattcggga
    13441 attgactaga aaggtcgaga aaacgatgga aagcaatggg ctgtgactag caagcatgct
    13501 gactgtgata aattgggcag aaccagcata gaccagcagg gacatcaacg tgacgactag
    13561 tggatgaaat ccggcagctt tcccaacgat accaaatgca agtccaatac cgatataacc
    13621 gaaaacggta ggtaacgtgt ctttgatcgc agtttttata tctaactttt catccaataa
    13681 caaaactcct ttagcacgac ataaattttc ataattgtcg ttaaacttgt gaatattcta
    13741 aatttttaat gagcatttgt caatggtggg attgaaacgt aaagaactag cgggtcgcag
    13801 atttacacgt attgtttcgg tgggaggagg cgcgaaaaat cttgcatggc tacaaatcca
    13861 agctgatatt ttcgatgcaa cggtctattg tttaagtgtg gaggaaggac ctgcattagg
    13921 cgcaacaatg attgctgcac tagggttagg gtggttcgac acacctgaaa gttgttgtca
    13981 gaattttatc caatatacag atgcagtgga acctctctta caaaatgtag aaaagtatca
    14041 taaaatctat caacaatgcc ggaaaatcta tccagcaacg aaggaaattt gtcatgactt
    14101 ataaaacaaa acgcgtacct aatagtcaaa tgactaatat tgggtacgcg ttaagtcttc
    14161 tttacttcat cctttgcatg attcgtgttt ttaaagcaag gtctgctgta tttcttgtgg
    14221 atttaggatg gttgttatcg tttacttgct atttttcatt gaaatcgtct ttctttaccg
    14281 tttctttagt tttttatcta tttttttctt ttggttagaa aattcttttt cccgaaagtc
    14341 ttctttcagg atttttatag agtagcgttt tgaataagag gcgtcagtat gatcgacttg
    14401 gcagacgaac caagagaaca tttgcctttt ttctgcatga ggtgcttctg ctaataattc
    14461 tttccataaa taataacagt cctctatcag aagaagggtt cgttcatcct caggctccca
    14521 atagaatgtt tcgagagtag caatcacttc attgatcaat tcgaatgcgg gaagataggc
    14581 attttgctga ataagcgatt caactgcttc ttggatgaac tttcgcatct ccatgatgaa
    14641 cttcgctgtt ttcctgtttt tgtttttctt gataatatga tcgtatttat ggatgatagt
    14701 tgttaattta tctgaataat ttattgcatg aaatggcatc ttaactttga agcgttcgat
    14761 aaagtgcgga tgttctataa gaatctctgt taaaagctcc cgtacaagag aggaactagc
    14821 ggaggaaaca agggacgaga gttcggaaag tgagtagttg gattttttta ttggacgttc
    14881 ttcgtaagcg aataagacag cagccatgtg tttgcatttt tttccttcta tggcatatag
    14941 acaatcacaa gtcatgttgg tcttttcatc ccaagtgatc actaccttat aatcttccgt
    15001 gccagaaact gttgcggaca agtacttgct tgtatagttc aaatgacgga caaaaccgtg
    15061 cagatgatag ctgtaaccgc gttccaaaac gttttcagga aaatactcaa gccaattatt
    15121 catctagttc tcctttcatg aaacacttat ttatatttag tataaagaaa cgtttaagaa
    15181 aaaagtagct gattttacat atttgataat cacttttgct cgaattgtca aaaattcatt
    15241 ctccttttct tttcttagag aatgtgagaa aaagtaaaag aatagcgtgt tttaaagatg
    15301 gaactttttt tgttttttat ttatctgaca aaaatgaagt gattttgtta gaaataaata
    15361 attgttccaa ttgataaaag aggtatgatg tatcgcaaag a
//
LOCUS       DASHRQ010000064        15168 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.068, whole genome shotgun sequence.
ACCESSION   DASHRQ010000064 DASHRQ010000000
VERSION     DASHRQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.068"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            278..787
                     /locus_tag="U2M88_002200"
     CDS             278..787
                     /locus_tag="U2M88_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM8374555.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="U2M88_002201"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="U2M88_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEM8374556.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="U2M88_002202"
     CDS             2322..3302
                     /locus_tag="U2M88_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8374557.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="U2M88_002203"
     CDS             3424..4842
                     /locus_tag="U2M88_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEM8374558.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="U2M88_002204"
     CDS             4913..6382
                     /locus_tag="U2M88_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM8374559.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="U2M88_002205"
     CDS             6392..6859
                     /locus_tag="U2M88_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM8374560.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="U2M88_002206"
     CDS             6895..7767
                     /locus_tag="U2M88_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM8374561.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..10383
                     /locus_tag="U2M88_002207"
     CDS             7849..10383
                     /locus_tag="U2M88_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEM8374562.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10539..11312
                     /locus_tag="U2M88_002208"
     CDS             10539..11312
                     /locus_tag="U2M88_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM8374563.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11305..12096
                     /locus_tag="U2M88_002209"
     CDS             11305..12096
                     /locus_tag="U2M88_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEM8374564.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12106..12597
                     /locus_tag="U2M88_002210"
     CDS             12106..12597
                     /locus_tag="U2M88_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8374565.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12615..13025
                     /locus_tag="U2M88_002211"
     CDS             12615..13025
                     /locus_tag="U2M88_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM8374566.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13029..14429
                     /locus_tag="U2M88_002212"
     CDS             13029..14429
                     /locus_tag="U2M88_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HEM8374567.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14452..14826
                     /locus_tag="U2M88_002213"
     CDS             14452..14826
                     /locus_tag="U2M88_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEM8374568.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5270 a         2129 c         3037 g         4732 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gcggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttttaacgca
     9841 ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat ttgtgttctt
     9901 tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa tctaattaat
     9961 gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg tgagttctgc
    10021 ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct tcgcttttta
    10081 gattccaaaa aagtattcaa tgagatagaa aaagtactat atttgtatga aaaatattac
    10141 gatgtcaaaa tcccaagttt tctgagaatt aatctatttc tacatctttc tggaatgatt
    10201 gaacgaatta tgattggtga tggaataaca aatgaacatt tgaaagaggg agcgcaacta
    10261 tttgatactt ttttaaatgt atcaaaagat tttttttcag aaatcaaaga tatttactgt
    10321 attgaaatac ccaaaggaga gtatgagtta atctatacaa tttttgagca gactattttc
    10381 taaatagcat actagcgtct aaaaaaatga aagggtgtcc aaagtatatt tagacgctca
    10441 tttaattaat atttactgta ttttcccgct ttattggata atgttagttg gcacagtaat
    10501 tgcttatata aaagtataag aagaaaggag agaacaaaat ggcgttttgg caaacatttt
    10561 tagttgtgtt gtatggattt ttcattaact gggataagga agggcccaaa atcggaacct
    10621 cccaacctgt cgttgcaggt ttcttgaccg gacttattat gggagatatt caaactggac
    10681 tttatatcgg tgggactttg caattaatga cactaggtat tactagtttt ggtggtgcat
    10741 cagtaccgga ttatcaaaca gcagcattga tcggtactta tttagcaatc tcaactaacc
    10801 aggatgcttc tataggaata actttagcaa taccggtagc aatgttaatt gtacaactag
    10861 atgttctaaa gtggtcaaca aacatttttt ttcaacaaaa ggcagaaaaa ttcgcacaaa
    10921 aagaagatta tcaaaaatta gagtggatgc aatatttggc atcaattaat acaagctttg
    10981 tatctggaat tcctgttttg ttaactgtga tattaggccc aacgttagtg ggaagtgcaa
    11041 tcaattatat tcctgaatgg gttaccggtg gtttaaaggt agcaggggga ctattacctg
    11101 ctgttgggat aggaatgcta cttaaatatt tacctacaaa agattacttt gcctatttgt
    11161 tagttggttt cgttttggca gtttatttcc aaataccaat tttagggatt tcgttacttg
    11221 gcgctgcaat cgcactaatc gtatataaaa atagatcaaa taatactcaa gtagcaccag
    11281 ctgcaacagg aggttttgac gaagatgaat aagaaactga caaaaatcga caaacaagta
    11341 attttccggt ggattctcaa tggtgcaact tcattaaatt atgaaaaaat ggaagggttg
    11401 ggttatgcgt attctatgct tccttttatc aaggacacat ataaagatga tcctgaagga
    11461 atgaaacaat ccgttttagc tcacctacaa ttttttaaca ctacgccgta tacagcgcct
    11521 tacattattg gtatgaatgt tggaattgaa aaagaagaaa aaaataagtc tttggaagca
    11581 gtaagtgctc tgaaaacagg tctaatgggt cctttagcgg gaattggaga tagtgtattt
    11641 gtagttattc catggactat ttttggagca attgcagcta atatggcatt ggaaggtagt
    11701 tttgttggaa ttattttgtg gatactggca tcaattttat taaggattac tgcttatcct
    11761 ttataccttg ctgggaaaga atcgggtgcg agcctactaa ctcaaattga aacaaaatta
    11821 aagtctatta ctgatgcagt atcaatttta gggttaatgg tggtcggtgc actcattgcc
    11881 acagttgtaa aagtgaatgt tggctttgaa tttaaacaag gcgacttaac aatgaccgga
    11941 aatgaaatct ttgatcaagt tatgcctggt ttattacctg catgtgtagt agcaattgtg
    12001 tattatttgt taggacgaaa ggttaaacca gtttatgtaa ttctattaat cttagtagtt
    12061 tctattgcgt taagagcgtt aaatattttg aagtaggtgt tgaatatgga tgaaaaaatg
    12121 aaaggtatta tacatgttcg tattgatgat cgaatgattc atggacaagt agcaacacaa
    12181 tggacaaatc aattgagtgc taacagaatt atggttatta ataatgaagt agccaatgat
    12241 gacgtaaaaa aagctgtagt tcgcctcgct gcaccaccta atgttagaac ttcaataatt
    12301 acaagggaag tagcagttaa aaatattctt tctggtaaat atgaaggaca aaaagttctt
    12361 attgttgcag tttctccaat cgatgttaaa tttttgattg ataacggttt accaataaca
    12421 agtgtaaacg taggaaattt atcaagaagg aatggaacag aacgcatccg tccgacgata
    12481 aatattactg aagaagagag agaggctttt aaagaactaa ttagtgatgg agttgaagtg
    12541 acagtgatac aaactcctag agattcaaaa gaatttttaa aggattttat ttattaagaa
    12601 cagaggatga gtggatggag tttataatta catctcatgg aaatattgcc attggatttg
    12661 tagatacatt aaaaatgttt tttggcgata atttaaattg tcatgtggta acacttggag
    12721 atgcaggcat tgaagaattc cgtgaaaaca ttgaaaaaat agtttctcaa gtaaaaaatc
    12781 aagaagttat ggtatttgct gacttgattg gtggaacacc tttcaacgaa tttgctaaaa
    12841 gaagtgaagt atttcaaaat gggtttcaac ttcttggtgg catgaattta ggaattttga
    12901 ttgaggctgt caatttgaga ttacaaggaa aaaaaagcga tgatatcatc gaaaatctaa
    12961 gaaatatgaa tacaattgcg tgtcttgcag atatgcaaag acatactaac gaagaggatg
    13021 agtgataaat gcaaaaaatt ttttacaagc ctaaagattt ttggtttggg gattgcatgc
    13081 ctttttataa agatggaact ttttatctat ttcatcaacg agatacgaga aatcctaaac
    13141 ctttaactga tccttttggg tggtcactag ttacaaccac tgattttgtg aaatttgaag
    13201 aatacgggga agtaattcat cgtggtggca acgatgacat tgatcaatac atttacgctg
    13261 gaagtattgt ccaaggaatc aatgagtatt tagctttgta tactggacat aatcgcaaag
    13321 ctaagttgga tggaaaaact tcagaagtgt taatgattgc aagttctaaa gatttaattc
    13381 attggaaaaa ggatggcgaa gcagcaaaac ttgttcctca agaaggatat gataagaatg
    13441 attggcgcga tccttttgca atttttgatc aagatcatgg aaaatacgtt ttgatactag
    13501 gagctagaaa accagcaagt aagaaaaagc cgacggggag attggtctat tttgaatccg
    13561 aagatttaaa agattggaat ttcaaaggag actactgggc taataatgaa tttaacatga
    13621 ttgaaatgcc tgatatattt gaaattgatg gaacgtggta tttacttttt tcagagtatt
    13681 gtgaagataa aaagactaaa tatcgagtcg gagcaggatt attttcagat tggaaatcac
    13741 cagtagacga agctttcgac ggaaaagcct attatgcagc aagaacagtc ggaactaatg
    13801 aaactggccg ttttctaact ggctgggtgg caacaaaaga aggggagaac gatcaaaata
    13861 actgggattg gggcggtgca cttgttccac atcaaattat tcaaaagaca gacaaaactc
    13921 ttggtgttaa actgcctgaa aaaataattg agaattttaa agaaatcact ccattttcaa
    13981 gttttaagtt agagaaagaa tttggaaaaa aagaaagagt aattgttcct aaaatcgaag
    14041 attattttct atttgaaaca actttgacta ttacatcgtt aacaaaggag tttgctttaa
    14101 aattatttgt agatagtgat actcaagaag gatacgaatt tcgtatttca ttgattaata
    14161 atcagcttac tttcggaaaa actccgaact atccttggcc tcaaatgttt gataaggggc
    14221 tgcaacgacc actatttttg gaaataggca aagaatatga actaaagtta attgttgatg
    14281 atacgatagc tgttttatat attgataatg ttgctttaag tactagaatg tatgataaag
    14341 tcggaacagc attaagtatg actgttacgg agggagcaat caaggtagat aaactagact
    14401 attcaataaa ttatgaatgt ggattttaaa acaattgaag ggatgatgta agtgaaatta
    14461 aatggtttac aacatattgg aattccaaca aaagattttg gagcttcaaa aatcttttat
    14521 gaatccttag ggtttagtct gattaatgaa gaaatgaatg tgggaagtaa agtcggcttt
    14581 ttcgagttga atggaacgat aatagaaata tgggaagata aaactactga gtgtagagga
    14641 gccattgatc atctagcatt agatacggat gacatagatg aggtattcaa ttcaattcaa
    14701 gatttaggat atgaattatt agataaaaat attatggaat taccgttctg ggaaaatgga
    14761 atccgttact ttaatttcta cggaccaaat catgagatta tagagataag ccaaatgaag
    14821 aaataaatag ataacgaagt gctctaagtt ggtatgaacg aaaagtactt cattttttgg
    14881 tatcagcatt tcaatagttt aatgagaatt tactatttca aattaattca aagtgacagt
    14941 attttttact tatttggttt caaattttgt aatccttcct atttattgga atacactcca
    15001 cattttgtat acaagtcata tcatgtttta gcaaagctct taccaagcaa agttataatg
    15061 atgttttcca aagtttgatc tttcgatgga ggctaacaca tattttttgc aggacagcca
    15121 aaattagatg tatagaatag agtctgtgag gcgtatagac agtttatt
//
LOCUS       DASHRQ010000065        14840 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.069, whole genome shotgun sequence.
ACCESSION   DASHRQ010000065 DASHRQ010000000
VERSION     DASHRQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.069"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            418..1032
                     /locus_tag="U2M88_002214"
     CDS             418..1032
                     /locus_tag="U2M88_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEM8374569.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            1805..3544
                     /gene="yfcC"
                     /locus_tag="U2M88_002215"
     CDS             1805..3544
                     /gene="yfcC"
                     /locus_tag="U2M88_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEM8374570.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            3701..4885
                     /gene="iadA"
                     /locus_tag="U2M88_002216"
     CDS             3701..4885
                     /gene="iadA"
                     /locus_tag="U2M88_002216"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEM8374571.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            4996..5199
                     /locus_tag="U2M88_002217"
     CDS             4996..5199
                     /locus_tag="U2M88_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEM8374572.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(5300..5962)
                     /locus_tag="U2M88_002218"
     CDS             complement(5300..5962)
                     /locus_tag="U2M88_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8374573.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(5975..7477)
                     /locus_tag="U2M88_002219"
     CDS             complement(5975..7477)
                     /locus_tag="U2M88_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8374574.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8025..9704
                     /locus_tag="U2M88_002220"
     CDS             8025..9704
                     /locus_tag="U2M88_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEM8374575.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            9831..10310
                     /locus_tag="U2M88_002221"
     CDS             9831..10310
                     /locus_tag="U2M88_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEM8374576.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            10529..11209
                     /locus_tag="U2M88_002222"
     CDS             10529..11209
                     /locus_tag="U2M88_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEM8374577.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            11217..12548
                     /locus_tag="U2M88_002223"
     CDS             11217..12548
                     /locus_tag="U2M88_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEM8374578.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            12563..13525
                     /locus_tag="U2M88_002224"
     CDS             12563..13525
                     /locus_tag="U2M88_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEM8374579.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            13636..13974
                     /locus_tag="U2M88_002225"
     CDS             13636..13974
                     /locus_tag="U2M88_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEM8374580.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            14053..14760
                     /locus_tag="U2M88_002226"
     CDS             14053..14760
                     /locus_tag="U2M88_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEM8374581.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4762 a         2578 c         3192 g         4308 t
ORIGIN      
        1 tttttttgta ttttcttgct tgcacttgga taagagatta cttacaataa attcagatag
       61 aaacagctga ataaaaaaag gctccctgac ccttagttgg cgctaagaat cagggaatga
      121 ccagtcacac acatacaccc tgaccgatca ttttgccata gtcaatgcag tgcatcgacc
      181 taatttattg tacctaatct agggtgaaaa ttctacccct atgtagatat tttccatggt
      241 ctgcacgttg ttttatttgc tatgtcaagg tattgggcgt attcgttcaa tgcctttttt
      301 gtgtttgaaa cagcaaagac cacatgtgtt actgtctaac tactgttttt ttatgtactt
      361 ccttagatta ggcagctgtt tctattcatg tcaagtatag ctaggcggga gagataaatg
      421 agtttatgtt attttgacta ttatcaccaa ttggaagaga aattaaggat agaagagccg
      481 ctgatgcttc aagctggcac ttggatcgcg aaacaaatta atcaaggtgg tagattgcag
      541 atatatgcta gccgcacact aagtggtgta gcatttgaat tttgggatca aaccccggat
      601 cttgtaccaa gtatattgat tgaaaatcca gccggaggtg tctatgaaac gctagaaggt
      661 gcaggacagg caattattga tcagctttct gtcaaatcag aagacatttt tcttcttctt
      721 tcgaatgaag gaagaaaccc ggcaatcatc gaactagctc agtggatcaa agaaaatggt
      781 catccattaa tcattgttac tggatttgat ttgtcacgaa gtatcaaatc gcaacatagc
      841 agtggtttgc gattatttga atttgctgat ttggttttgg acaaccacgc caatttacat
      901 gatgcagtct tgactttgcc aaatctagat ccagcgatct gcggaacagc ctctcttgca
      961 acgatctttt tgctgcagca agttttgtat tttacagcaa aacagctagt ccaggacaca
     1021 agtggaaaat agttatccac atgttgacaa agttccttaa aaaaataatg ttccacataa
     1081 aaatggagcg tttgaaaaaa agaaaacgat tattaatgag gattaacaac gactactttt
     1141 tttgttttag ttattttaag atggatcatc tttgatttta agcttgtttt gtttaaagtt
     1201 tgttattttt ataagttgtt tcacagttat gagaaatgag ttataagaat atgtagtgtc
     1261 agaaaaataa aaagtagtgg aaaaagagac aagaaagaat ccatatggat tctttcttgt
     1321 ctcttaatac acgttgatga aagagtcttc cccaaaactc tttacaaagt aattatagta
     1381 catttgttat cattattgca atgaagttta cctgatataa caaaattgtc caacaggata
     1441 cctttttcta atagaaataa aatacacaaa aattgaagga tagataatgg atatttataa
     1501 gtgtttatta ttttttttgc aatgaaattc aatttttcaa tgattgtaaa atgaaaaaaa
     1561 caggacgaga ttttctctaa tctaaaaata aaaaaagaat tttaaggaaa aacagagaaa
     1621 taaaatactt aatataaatg gaaagagaaa aaaataatca tacataggag aaaaaaaaca
     1681 aaatagaaaa aagtgatttt ctaataaatt attcatgtgg aaatgtcatt tttactgtat
     1741 aattcatttg attatccatt ttttattcat caaaattact gaaaaaagaa aggaagaaat
     1801 aaatttgaag aaaacaaatt cttcaggaaa accgaaacgc gacttcacta aattatcgac
     1861 accccatacg tatgtcatta tttttggtgt agtcatcttt gcatggatac tgacttttgt
     1921 cgttccagca ggaaagttca gcacgcaaga tattgaatac aaagatgcaa atggcgaaac
     1981 aagtactcgt actgttttac gtcaagattc cttccgttat gcttacgagc tggataagtc
     2041 gtatgtgttt gatcaattag aagaactgca agatcatcca gcagaacgtg aaaaactgga
     2101 tgttccggaa aaaggattgg aaaaagtcat tgctgacgga gaaaaaaatc ttacgcagga
     2161 aaaactagat gaaatctcct tgacagatga tgtgctttac gatcagtacg gcgaaaatat
     2221 ctatgatact tctaaaaaat tgcataaaac agccaaaata tggggaacag atgattttgg
     2281 cggctttggt ttcttaaatt ttgtttttga aggactagta tctggagata agtatggatc
     2341 tgctgtaggg atcgctgcat tgatccttgt tgttggaggt gcttttggta ttattatgcg
     2401 taccggagcg atcgacgcag gtatctatgc gtttatcagt aaaactaagg gactggagcg
     2461 tttagcttta ccgctattat tctttgcttt ttcattcggt ggcgctacat tcgggatggc
     2521 agaagaggta attccattct caatggtgat ggttccattt gtcattgcgt taggatatga
     2581 ctcgatcgta gcagtaaccg tgacttatgt cgcatctcaa gtcggaaatg cgacctcttg
     2641 gatgagcccg tttagcgtag cggtggctca agggattgct ggaatccccg tgttgtctgg
     2701 tgcgacattc cgattgatta tgtgggtagt tgttactgca ttagccgctg gttatatgat
     2761 gatatatgca gaaaaaatcc gtaaaaaacc agaaagttcg ttgacttaca aatcagatga
     2821 ttatttccgt tctcatatca aaaagacatc tgatgaaaat aaaaaattca tgcttgggca
     2881 taaattgatc ttattagaga tgttagttgt ccttgtctgg atcgtttggg gcgtgactca
     2941 aaaaggctat tacattccag aaatcgcttc tcaattcttt gtgatgggat tagttgctgg
     3001 aatcattgct gttcttttca aattagatgg aatgaaagtc aatgacatcg cttcttcttt
     3061 ccaatctggt gcagctgatt tagctggtac agcaatcgta gtaggtatgg ccaaagggat
     3121 tcttcttgtc ttgggaggat ctgatgcatc tgttccttca gctttgaata cgattttgca
     3181 tgggatcggg acggcgttga ccggtgttcc agctgtcatt ggtgcttggg cgatgtacat
     3241 cttccaaagt ttgttcaact tggttgttac atcaaattca ggacaagcag ctttgactat
     3301 gccaatcatg gctccactgg cagatttagt aggtgtatcc agacaagtag cagttcttgc
     3361 ttatcagctt ggttcaggtt ttatggacgc ttttacaccc gtatcagcca gcttgattgg
     3421 tgtgctaggt gttgcacgga tcgaatgggc aaaatgggca agattccaaa tcaagatgca
     3481 aggattcctg tttgttttag gaactatttt catcatgatt gccattgcaa taggtctgca
     3541 gtagtactgg agacctgtaa ataaaaaagt aatccaaaca gtagataagg actccaagaa
     3601 ccgtcatttt acggtaaaat caaaggagta aagatcctat cgtttggatt attttttaag
     3661 atcctatcat cagttatact taaagaaaga ggaaaaatgt atgaaaatca ttcgacaaat
     3721 tgaagtgttt gcaccagatt acttaggaaa aatggatgta ctgattgccg gtgggaagat
     3781 tttggctgta gaagaacagc tagaaggcgg ctacgaagga gtggaagtag aagaactttc
     3841 aggtgaggga aaagtactaa ccccgggttt cattgactgt catttccata ttttaggtgg
     3901 aggaggagaa ggcggttatc aaaatcgaac gccagaagtc acattgagcc aattgactac
     3961 agctggtgta acgacagttg tcggttgttt gggaacagac ggagaaggta gagatatgac
     4021 tgcgttgatc agcaaagcga aaggtctgga agcagaagga atctccactt atgtatacga
     4081 aggttcgtac cgattgccgg taaaaacagt aacagattct atcatcaaag actttttgac
     4141 aattgataaa attatcggaa tcggagaaat cgcagtatcc gatcaccgtt cttctcagcc
     4201 aagttttgaa gagttcgctc gtgcagtagc agatgctcgt gtaggaggca tgttatccgg
     4261 aaaagctggg atcatcaatg tccacttagg cggaggaaaa agaaagctag agctattgac
     4321 acgaatcgta gaagaaacag aaattccgat cacacaattt ttgcctactc atgcaaatcg
     4381 cacaccagaa ttatttgaag cttgcgtggc atttgccaaa cgtggtggca cgattgactt
     4441 cactgctagc gaagatcctg atttttggga gaagacagat ggtgaagttc gcttcagcaa
     4501 agcactgaaa cgattgatcg aggaagatgt ttctttagat aatttcacga tgacctcaga
     4561 tggacaagga agcttgcctt attttgatga aaacaatcat ttcctagggt taggagtagg
     4621 aagtgccaag gcattgctag tagggatcaa agaagctgtg caaaaagaaa gtatcccttt
     4681 ggaaatcgcc ttacgagcta taaccagtaa tccagcacgt atattgaaat tggataaaaa
     4741 aggaaaaatc gaaattggtg ctgatgcaga tctgtgtatc ttggataaag aaacgcttga
     4801 tattgataca gttatcgcta aaggagaaat aatggtccaa gaaaaagaag tcaaagtatg
     4861 ggggactttc gaaaaaagtt tttaatcaac agctctaact atctttttta gaaaacgagc
     4921 gttctgtttg tctgtatctg ttgttgatgt taccctgaag gtagatcaat aaacaaacta
     4981 agaggaggaa gcacaatggc agatttaaaa ggacgttcta aagatatgaa agataaagta
     5041 gtcggtgaag gaaaagaagc atacggcaaa gcaacagacg acaagagcaa agaaacagaa
     5101 gggaaagcac aatcactagg tgctgacatc aaagaaaaag ctcgaaattt aggcgacgaa
     5161 gtcaaagaca agttttctcg aaaagacgaa aaaaaataaa aaccacttat tactaattga
     5221 atcaagcaaa taaaaaatcc gaaccatatc tgcgagacag atatggttcg gattttttat
     5281 ttgatcatta tcctgttttt taaacttcga ttttttgaaa ttttttattt tttagttcaa
     5341 agacttcatc acagcttttt gctacttcaa tctcatgagt cacgatgata atacagcgat
     5401 tttgttcatg ggcgatttct tggaaaagtt tgacgatatc ttgtgaagtt gtttcatcta
     5461 gatttccagt aggctcatcc gctacgatta gttgatgttc acagcagata gcacgaacga
     5521 tagcgactcg ctgctgctga cctcccgaaa gtttggtcac ctgttttttt gctaatattt
     5581 catcaattcc tacacgagcc agtcgatcta aagcaacttg tcgataattc tcttgcttgg
     5641 tcttagtgat ttccattgct gctatgacgt tgtctagagc cgataaataa ggaagcaagt
     5701 tgtaagcttg gaaaataatc gagacatcat ttttacgata acttcttaac ccgattttgt
     5761 tcagtgaacg atcttgatag tatacctttc cttcttttgg tgcatctagt ccagaaataa
     5821 gcgaaagaaa agtcgttttt ccagaaccac ttgctcctaa aatagcgtac atttttccag
     5881 cttcgaaatt cagttctaca tctttataaa gcgcatcttg cggatcttga taccaaaatc
     5941 ccaattgttc tgtccgtaac atcaaattcc tcctttagtt taataggatt tttcttgggt
     6001 ttaatcttaa tatccctata gaagctaaaa agacagaaaa cagactgatt ccaaatgcta
     6061 ctccggctaa tagtcccaat tgtgcaggtt tgacattgat ttccaattcg ctgacttgat
     6121 cagaaacagc aaatggatta ttcgtggttc cttgttgacc atcgcgttga ccgccttgtt
     6181 cttgtctgtt acctggcatt tgtccaccgc caggagcttg ctggttttgt gtcgtggttt
     6241 ctgtctgttg gtcaagtagc tgctgtccca cggcattcgc gacgacgttt ccgctaagcg
     6301 aagcaactcc taatgcaaga atcatacaaa tagccacttc ggtaaacaat tggagaatga
     6361 ttttacttct ggattctcct aaagatagca agacgcctaa ctcatgccga cgctcccgaa
     6421 tggtaatcat aatgatcaga gtaagtatga taataccggc tacagccact agtaaaacaa
     6481 catttttaga aaaacttgcc acgttgttta aaggttccaa cattgactga tacatggaat
     6541 catttgattg taagctaaat gtatctgtgt cgatcagctt ctcagcttct ttgagaaact
     6601 catccatttt atctggatca gatagagtgt aaatagcaga atctatcgtg ttatcgtcag
     6661 atgtcccatt tagttcattt gcaaaagtgt aagaagtgta aagtgtattc gacggattca
     6721 tgaagttgaa tttcataccc atactgccgc ttgtttcgct ggattcataa attcctttga
     6781 tcgtcatttc ataagtggta tcttcatctt cagtactcgt gattgtgaaa gtatctccga
     6841 ctgccaggtc atttgtttct gctagagtag aatcaatgac tacattgttt gtgcctttgt
     6901 cgctgtcatc tattccttca ccatccgtga tttttgctgt tccatcagaa aaattactat
     6961 tttgtgcagt tgctgacaca ccactgattt gaaagtctgc ttgagtcatt tgcggattcc
     7021 cgcccattcc tccagcaggt tcaccgcctg catcagtagt ttctgaactg tcttcacttg
     7081 aatcagaact agtgatggca gttattccat cttttgccat agctgaggcg gaactttcaa
     7141 aggaataact ctttacatta tctaattcag ctattttttc cgcgtccgtt aaggaaactg
     7201 gtgtaagaga aaaactgcct ggatctggac gttcttcagt ggtagaaccg cttgaagaag
     7261 tttgttcctg ttttttaaat gaagcttctc gattggcact taaagtcact gttgctccaa
     7321 cttctttttg tgcttgattc gctgcttctt ctgcagcgct tcgaatcgtg aggccagcta
     7381 gcacaaaaat aagaatggct gtgaacacgg caaacaataa caatgaacgt ccccatttta
     7441 cttttgtact tttccatgca cgtttcataa aattcatatt ttgcatccct cctagtagat
     7501 gcatttgata atagacaacc tatcttaaag ctagcttaaa tcttatgctg tttgcacacc
     7561 atcaacatga ggttctgtat atataaaaag tcactatagt tattgcaaaa aatgtggtaa
     7621 aatgaaaaaa gtatgtttca aaccgacgaa gaagaaatga aagaggaata gcaaaagaaa
     7681 tcctagatgc tacagaggga tttttacgtg catagttgtt ctaacaattg ctgatactag
     7741 cgatttagtt tatttgttgt ataataatgg ggaacagctg aatacttata taagtattct
     7801 ggctatagcg tttcgcaaaa gatccatttt gttaatgaag tatatccaaa ataaaatgcc
     7861 catccaactg tcagaacgcg gatgtgctgg taattttcct gctgattctc cataagttga
     7921 gaaatagtga ttttgcatat gcttcattgg aatggctcat atatttagat aaagaagaaa
     7981 atcgacatct ttaaacttgg atgtcataag aagaggtgaa gtttgtgagt accattaaaa
     8041 ttatcccatt aggcggcgtt cgtgaaaatg gaaaaaacat gtatgtcgca gaagtgggcg
     8101 aggatatttt tattttagat tgtgggctaa aatatcctga aaatgagttg ttggggatcg
     8161 acgtcgtaat ccctgatttt acgtatttag aagaaaacat tgatcgtgta gctggtgtct
     8221 ttttaacaca tggacatgca gatgcgatcg gggcacttcc ttacttgttg tcaaaaattt
     8281 ctgtaccagt attcgggaca gagttgacga tcgagttagc aaaattgaat gtcagtcgta
     8341 atgaagcagc caaaggattt aaggatttcc atgtggtaga tgagcataca gaaatagact
     8401 ttggtgaaac agtagtgagc tttttccgta ccactcatac gataccagat tcagtgggga
     8461 tcaatctgaa aacaaaagaa ggtagtatcg tttatacggg agattttaaa tttgaccaaa
     8521 cggctatccc aatgtatcaa acagattatg cacgattggc ggaaatcggc aaagaaggag
     8581 tcttagcctt actaagtgaa tcggcaaatg cagaaaatcc tcagcctgta gcttctgaag
     8641 cccaaatagc agatgaagta tttgatacga tcaaatattg ggaagggcgt atcatcgtcg
     8701 cttgtgtagc aagtaatttg caacgagtgc aacaagtatt ggatgcagca gaaaaagcac
     8761 aacgaaaagt tgtcttgact ggtcaagatt tcgaacgaat tattcgcaca gcgatgcgtt
     8821 tagaaaaact tcaattacca agtgaagatc tgcttgttac gttgaaagac atgaaaaact
     8881 atgatccaga agaactgatc attttagaaa ctggccgaat gggtgaaccc atcaagtctc
     8941 tgcaaaaaat ggcaaacggt acacaccgta atgtcaagat tgaagaagga gatttggttt
     9001 atatcactac tacgccaagc atcgcgatgg aaaccatcgt ggcgaaaacg gaagatatca
     9061 tttatcgagc tggtggaaca gtgaagttga tctctgagaa tatgcgcgtt tctggccatg
     9121 ccaatcctaa tgacttgcaa ttaatgatca atttgatgaa accaacatat tttgtgccag
     9181 ttcaaggaga gtatcgtgaa ttagctgctc atgcagattt ggctcatgca gtagggatgc
     9241 catacaaaaa tatctatatt actggacgcg gagacgtgct ggaatataag aacaagcgta
     9301 tgagtgttgc aggaactaca agtgcagaaa atgtaatgat tgatggatta ggcataggtg
     9361 atatcggaaa cattgtttta cgtgatcgaa aaatcttgtc agaagacggg attttcgtag
     9421 cggttgtcac tatcagtcgg cgagaaaaga aaatcatttc aaaacctcaa attacttcac
     9481 ggggattcgt ttacgtaaaa gctagccgtg atctgatgaa agaaagcagc aatatgattg
     9541 aaacaatcgt tgagaaacat ttagaaagca atgattttga gtggagcaaa ttaaagcaag
     9601 acatccgaga tcaattaggc cgttacttat ttgaacaaac aaaacgccgc ccagtgatct
     9661 tgccagtcat tatggaagct actcaacgtc gaaataaaaa ataaacaaaa agagtaagat
     9721 gaacctctct atagagtgct cgtcttgctc tttttttgcg aaaaacaaat aatttttgaa
     9781 tacagcatcc ctcgctttat aataaacgat agaataaagg gagtgaatca atgatgagtc
     9841 aaacaaaagt cgaacgttat ttagaagaac aaaagatcag ctatcaacca cttgatttta
     9901 gtcatgtggt agaagaatct ttcacagaag aactacaaaa aagagggatt gatccagctt
     9961 tgatatgtaa aactctcgta gtgaaaggaa acaaaactgg ggtgatcatc gcattgattc
    10021 ctttgaacga tcacttagat tacaaaaaaa cgagacaagt gacgaaagat cgtaaagttg
    10081 gttttcctgg aatggatttt gtattaatgc atacaggata tccccacgga gcaaacaccc
    10141 ctattggaat aaaactcgca catcctgatt attgtttttt agcagacagt tctttaaaag
    10201 aaaaagaaga agtaattgtg tcaagcggtg aaatcgggag aagccttcgg ataaaagtag
    10261 ccgatttaga aaagatcgtc catcctattt acgctgattt aattagttaa gggacgagaa
    10321 gagaagctaa gcagagcaga gacctcaaac ttgagattat atctgcttgg tacttggtgc
    10381 atcctgtatt atctgttaca attgctttgt acatactgta ctatcaatcc aatgaaaata
    10441 tatttagcag ctatattttt tcattgatgt gcaagtgatg aaatcgtttt taataaaaaa
    10501 gatacagaga aaacgcgagg tgtaaaatat gaaaattacg tatcatggac attcctgttt
    10561 aacaattgaa atggaaaatg gccaacgcgt gatgattgac ccgtttatta caggaaatcc
    10621 attgacagac ttaactgcag aagaagtaaa gacagattgg ttgctgatta ctcatggtca
    10681 tagtgaccac ataggagata tgattcctat tgcaaaagaa aatcaagcca caattatcag
    10741 tattgttgag attgctgatt atgcgaacag cagaggagta gattcattcg gtatgaatat
    10801 cggtggaaaa catgcgtttc cattcggcgt ggtaaaattc gtacatgcac agcatagttc
    10861 aagctatgaa gtagacggta tcgttcagta tatgggcgag cctagcggaa ttattattca
    10921 agcagaagga aaaacgattt atcatgcagg ggatactgct tatttcagtg atctaggatt
    10981 gcttgcagaa gaattcgata ttgatgtcgc tttcttgccg attggcgata actatacgat
    11041 ggggccagaa gatgcagcaa aggctgcgca aacaattcgt gccaagaaag tagttccaat
    11101 ccattacaat actttccctg tcattgaaca agatcccgaa tcttttgtag agttactgga
    11161 agatagtgaa ggaaaaataa tgagcgtctc ggaaacgctg gaagtgtagg cagaaaatgg
    11221 cgacaaaaca tgatttgatt ttagaatata ttgaaagttt acctgttggc aatcgaattt
    11281 ctgtaagaag catcgcaaaa gaattgaatg tcagtgaagg aacagcgtac cgtgcaatca
    11341 aagatgcaga aaatgtcgga ctagtttcaa caattcaacg agtagggacc atccgtatcg
    11401 aacgcaaatt aaaaaaacat attgaacgtt tgacatttgg cgaggtcgtc aggatcattg
    11461 aaggggacgt tttaggcggg gcggcaggat tagacaaggt cttgaataaa ttcgtcatcg
    11521 gcgcgatgac taaacatgcc atggaacgct atattacgcc aggatcacta atgatcgtcg
    11581 gcaaccgggc ggaagtccaa aaattagcat tggaagatgg cgccgcagta ttgatcacag
    11641 gaggattcaa tacgactgct gagattgcaa agctagctga tgatcttgaa atgccgatct
    11701 tacgcacctc ttatgataca tttacagtcg caaccatgat caaccgagcg ttaagcgacc
    11761 aattgatcaa aaaagatatc atgcttgtca gtgatatcta tatgccagct gaaaagactc
    11821 attatttgca gcagacagat acgatttctg attatcagcg tttgtcagaa gaaacgcagc
    11881 attcgcgttt tccagtcgtc aaccgccatc atcgtttgat ggggattgtt acggcaaaag
    11941 atgttttagg gaaatcgcca aaccagatca tcgatcgggt gatgacgaaa gaaccaatca
    12001 gtgtaaagaa aacaatgagt attgcctctg ttagccatca aatgatctgg gatgggctag
    12061 aagtcatgcc ggtcgtttcc gatgacttga cattggaagg cttgattacc cggcaagatg
    12121 taatgaaagc gatgcagctt gtccagcgac aaccgcagat tgcagatacg atttcggatc
    12181 agatatctgg aaatatccat acgattgata ctgaccgtga aggaaatcgt ttggaaaacc
    12241 ctaagttcaa attcgtagta gcaccacaaa tggtcaatgg cgttggaacc atttcttttg
    12301 gcgtacttag cgaaatcatt tctgatgtcg cacaaaaaac aatggtgatg aaccaaaaac
    12361 gaaatatctt gattgaacaa gtgaacctac attatttgcg gctgatccaa ttggaaagcg
    12421 aattagacat tcgtccacgt gtattggaaa tcggcagacg ttccgctaaa ctggatatcg
    12481 aagtgtttat tgaaaacacc atcgtttcca aagcaatcgt tgtttgtcaa gtaatggaac
    12541 gttcttagaa agcaggagtg aaatgacagt acagcaagaa atagtagata caataaaaaa
    12601 atacgataaa atcattatcc acaggcattt gcgccctgat cctgatgcat taggatcaca
    12661 agtaggatta gccgaactat tacgggaaag tttcccagaa aaagaaatac gtcaagtagg
    12721 tgaaacgatt gaagggcttc gctttttagc agaaatgcaa gaagttgaag atgtcttcta
    12781 ccaaggagcg ctagtcatcg tgactgatac agctaattca cccagaatca gtgatcaaag
    12841 atatcagttg gcagaaaaac tgatcaaaat cgaccatcat cccaatgatg acccttatgg
    12901 agatcttgtg tgggtaaata caaaagcaag tagctgtagt gagatgattg cagaatttgc
    12961 tttgatgttt cctgaatctt taaagatgaa cgttaaggct gcacggttac tttatgcagg
    13021 aatcgtcgga gatacagggc gctttttgta tccctccacg acgtcccgta cattagagat
    13081 tgcttcgata ttgagaaatt atccatttga tgcaagtgct ttgaacagag agattgaaca
    13141 aatgccgatg aaagtcgcta aactttccgg ttatctttat caaaatatcc aggtggatga
    13201 aaatggtgca ggaagaataa tccttccgca agaaattctt gataaatatg gaatcgatga
    13261 ttcagagaca gcttcgatcg tttcacttcc tggagtgatt gacgaagtgc tggcttgggg
    13321 gatttttgtt cagcagcctt cagggtatta ccgcgtacgt ttacgttcta aggggccgat
    13381 catcaatgaa ttggctaaac gccatcatgg aggaggccat ccattagcaa gtggtgcgaa
    13441 tgcaaaagat ttagcagaag tagaggaaat ctataaagag atccaagata tatgccaatc
    13501 gtataagagc agaaaaacag aatgatactt cgttcgaaaa cgtgcctttt gtagtaaaaa
    13561 tcacaaatgt atcttttcaa gatcttcttt ttccgttaca atagtgctca ctaaagattt
    13621 taaaggagaa agaagatgac attaccaaat tgcccagaat gtggctcaga gtacacatat
    13681 gaagatcgag gcttgttgat ttgcccagaa tgcgggaacg aatggaacaa agaagaacaa
    13741 gaaactgtca tagaagacgg attagtggta ttagatgcaa atggaaaccg tctgttagag
    13801 ggcgatagcg tgacagtgat taaagacttg aaagtaaaag gtgcgtcagg tgccattaaa
    13861 caaggaacaa aagtcaagaa catccgttta gtagaaggcg atcataatat cgattgcaag
    13921 gtagaaggat ttggaccgat gaaactgaaa tcggaatttg tgaaaaagaa ttaaagaata
    13981 ttcaaggtct tttcacattc ttttcctact actaatgata aaataaagta aacgaaaagg
    14041 agtgaaacga aaatgtatgg ttatggaatg tatggttttt tagatccgac atttcttttg
    14101 gtcattcttg gattagttat ttccggaatt gcgtcagcgt acgtcaatag tactttccga
    14161 aaatacgatc aagtgagaag caaaaacaat gtgactggta cacaagcagc acaatatata
    14221 ttacaaagtc aacagataaa taatgtcggt gttcaacaaa ttgccggaga tctcacagat
    14281 aactacaatt caggtaataa gatgttgagt ttgtctcaag caactgcaca gtctacttca
    14341 gttgctgcga taggtgtagc agctcatgaa tgcggacatg cggtacaaga tgcttcgggt
    14401 tatgtcccat tgaagattcg tgcagcactt gttccagcag ctaactttgg ttcgatgatt
    14461 tcgttcccct tgattatggt aggtgtattg cttagctgga actctacgtt aatcaatatc
    14521 gggattttcg ctttttcttt agcattactt ttccaattgg tgacattacc agtcgaattt
    14581 aacgcgtctc ggcgagcaat ccaaattttg agtgaaggcg gactactgac ggaagaagaa
    14641 gtaccgatgg caagacacgt attatttgca gcagcactga cttatgttgc agcagcgctt
    14701 tcgacattcc tgcagctgtt gcgcttgatc ttattgttcg gcggcaaccg aagagattaa
    14761 aataaagaag ttgtgaaatc agcgctttga tctgagctaa taccgagagt tagctgattg
    14821 aataccattt atttggaata
//
LOCUS       DASHRQ010000066        14821 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.070, whole genome shotgun sequence.
ACCESSION   DASHRQ010000066 DASHRQ010000000
VERSION     DASHRQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.070"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(626..988)
                     /locus_tag="U2M88_002227"
     CDS             complement(626..988)
                     /locus_tag="U2M88_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374582.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(1062..1442)
                     /locus_tag="U2M88_002228"
     CDS             complement(1062..1442)
                     /locus_tag="U2M88_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEM8374583.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1506..2699
                     /locus_tag="U2M88_002229"
     CDS             1506..2699
                     /locus_tag="U2M88_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEM8374584.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2776..3360)
                     /locus_tag="U2M88_002230"
     CDS             complement(2776..3360)
                     /locus_tag="U2M88_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEM8374585.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3561..4559
                     /locus_tag="U2M88_002231"
     CDS             3561..4559
                     /locus_tag="U2M88_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEM8374586.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4671..5180
                     /locus_tag="U2M88_002232"
     CDS             4671..5180
                     /locus_tag="U2M88_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEM8374587.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(5210..5869)
                     /locus_tag="U2M88_002233"
     CDS             complement(5210..5869)
                     /locus_tag="U2M88_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEM8374588.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5871..6635)
                     /locus_tag="U2M88_002234"
     CDS             complement(5871..6635)
                     /locus_tag="U2M88_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEM8374589.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6643..7320)
                     /locus_tag="U2M88_002235"
     CDS             complement(6643..7320)
                     /locus_tag="U2M88_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEM8374590.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7357..8742)
                     /locus_tag="U2M88_002236"
     CDS             complement(7357..8742)
                     /locus_tag="U2M88_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEM8374591.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8952..9036)
                     /gene="ffs"
                     /locus_tag="U2M88_002237"
     ncRNA           complement(8952..9036)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="U2M88_002237"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(9059..9574)
                     /gene="tadA"
                     /locus_tag="U2M88_002238"
     CDS             complement(9059..9574)
                     /gene="tadA"
                     /locus_tag="U2M88_002238"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEM8374592.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9681..10538
                     /locus_tag="U2M88_002239"
     CDS             9681..10538
                     /locus_tag="U2M88_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8374593.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10619..10954)
                     /locus_tag="U2M88_002240"
     CDS             complement(10619..10954)
                     /locus_tag="U2M88_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374594.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(11021..12391)
                     /locus_tag="U2M88_002241"
     CDS             complement(11021..12391)
                     /locus_tag="U2M88_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEM8374595.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12469..13005)
                     /locus_tag="U2M88_002242"
     CDS             complement(12469..13005)
                     /locus_tag="U2M88_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM8374596.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13314..14795
                     /locus_tag="U2M88_002243"
     CDS             13314..14795
                     /locus_tag="U2M88_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8374597.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4544 a         2943 c         2407 g         4927 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcggg agtgtttccc gattcgtttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa gcgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt ttaacgactc
      301 agttatctta tcatgtcgtt agtaacatgt caataactat tttttaaatt ttttagaaat
      361 gtgatataac atccacttca gaatcaacgc tgttaaattt atcatgtatt tcttccttgg
      421 tcaactataa attaagcatt actttgtgaa atgatttttc gatcaattat tattattcgt
      481 tatacaaccg ttttcaacat attttgaaaa taaaatgcac taaaaaacta tctttagcag
      541 aacatttcat tgtatttttt ggaccaaaca ataaaaaaag aagtttgaaa aaaattcaaa
      601 cttctttgtt cgcttaatct gtattttaga agaaaggcac tccctctact tcgtaagttc
      661 caagaacaga gcgcatttga cctggttctt cccaaatata tccttcgcct aattcttctc
      721 tttgattctt ttgttcagtt tcaaagacaa acaacggaac attttcctct ttaatatgtg
      781 tatttgtcaa ttccatcaaa ctaatcttgc taacatcgat atgaactatt tcttttaaga
      841 aattaagtat attcgctaaa ccagtctttc cttcagaaag ttccgctaac gcaaattcca
      901 acttgtcatc aattgaatgg accaaaaatt ttttagtacc atcctccaaa tgcaacataa
      961 ttgtaccagc aactcttttg cttgccaaaa tttccacctc gatcaacttg ttctatggct
     1021 tacataaaaa ttctagcaca ttttcagaaa aaatacactt tttatttctt attttctttc
     1081 cgttctagtt cagataatgc ttcttctatc caaataggta actgctccgc aatagtggta
     1141 aatccgatct tacgattttt attcgtgaaa tacctttttc tgcgatatgg aacagctgga
     1201 tttgtttttt ctaacgttct taaagataaa ctgatttttt tggaatattc atcaatgtcg
     1261 atgatctgaa ctgttactgg ctgtccgact tttaacaatg aatggatatt tttcgtataa
     1321 ccggattgta cttctgaaac atgaataagt ccttgcgtct cgttatcaag agaaacgaat
     1381 gcgccataag gctgtatccc tgtcactttt ccttcaatta tttgtccaat tttataagtc
     1441 atctacttcc ctctcttcgc atttatcatt atactgtaaa tagctcaaaa aaagaagcga
     1501 cgaaaatgac tggaggtctt aagatgatcg aatttgataa acagatttca agaaaagata
     1561 cagaaagtgt aaaatgggac gcaattgctg agacatatca aatggatgac ttacttcctt
     1621 tgtgggtagc agatatggac ttcttgtctc ccggtggggt agctaaagcg tttagtgaat
     1681 acatcaaaca tggaatcttt ggttatgcca ctctgtctga taaactatat caggctatca
     1741 ttcattggga acgccaacac catgctgtgg agttgacgaa agaaaatatc gtttttacaa
     1801 gtggtgtgtt aaccagttta gctgcagcag tacaaacttt tacaaaacca ggcgattcta
     1861 ttttgattca tgacccagtt tatcctccat tttcctctat tatagaaaca aatcaacgtc
     1921 acatcgttcg aagctctttg ctagaaaaaa ataatcattt catcatggac ttgtctgaca
     1981 tggagaaaaa aatcgaagaa aacaatgtaa aagcaatgat tttatgcaat cctcacaatc
     2041 ctggagggcg tgtttggagc aaagaagaat tgaaacagtt aagcgaattg tgtttaaaac
     2101 atcaagtgat tctgtttagt gacgagatcc atcaagattt aactttattc gatcatacat
     2161 ttacttccat gctaacgatt gatcctaatt tagatgatct actagttgct tttacttccg
     2221 caaccaagac tttcaatcta gctgcaatca aaaattcaat ggtatttatc aaaaatccag
     2281 agatgaaagc tgattttgaa aaacatttag tgatgaacca acagcacgaa atcaacactt
     2341 ttggactgat tggaacccaa gcggcttatg agacgggtgg agaatggtta agccaactga
     2401 ttccttatct agaaaaaaat gttcaaacag ttaatcagtt ttttgaacag tatcttccga
     2461 aagtgagaat aatgaaccct gaaggaactt acttaatgtg gttagatttt accgcctata
     2521 tttcagacga ccaagaattg gagaacactc ttgttcggaa aggaaaagtc gtattaaatc
     2581 ctggcatcac gtttggtcct tccggtcatg ggcatatgcg cttgaatatt gcttgtccag
     2641 aagaaacttt actggaagga cttaatcgaa tcaagtatgc tttagagaca atcgattaaa
     2701 tcatttattc aaaataaaag gacgagacct tactaggttt cgtccttttt cataattact
     2761 ccgtaccata ccaaactatt ctgaaatttc aattgtttca atcactacat catgtacagg
     2821 tcggtcttgt ggaccacgtt gtacatttgc gatttcatct actgtgtcca tcccttctag
     2881 tacatgtcca aagactgtat gtcggaaatc tagccaagga gttccgccac ctttatacgc
     2941 ttcaatgatt tctgatggga atccagcacc ttctagttgt ccaagcatat tagctggcac
     3001 atttgtgtta tttacaataa agaattggct tccgtttgtg tttggacctg aatttgccat
     3061 agataaagct ccgcgaaggt tgaacagttc acgactgaat tcatcttcaa aactttcgcc
     3121 ataaatactt tcgccaccca ttcctgttcc agttggatcg ccgccttgga tcatgaaatc
     3181 aggaataacg cggtgaaaaa tcacgccatc ataataacct tttttggaaa gttcaatgaa
     3241 attttgaacc gtcttcggtg ctagttcagg gaataattga acagtaatgt caccacggtt
     3301 tgttttgata acagcttttg gtcctttttc actttttaaa tctagttgtg ggaatgccat
     3361 attgtttcct cctaatattt gcatacttca taataataat aacataatac ctaacctctc
     3421 gtaaaaatac tagatttgtt ttcttttatt tttcatcact agatacattc tataacttac
     3481 cgtttcgatt tcgaattcaa acatttttga gaagcattct caattaaatt ctgctatagt
     3541 gtgaaaaggg aggaattttt atggaaatct atgacattac aatcatcggt gcaggtcctg
     3601 tagggatgtt tgccgctttt tacgcaggaa tgcgccaagc taaaacaaaa atcattgaca
     3661 gtctacctca acttggtggt caattagcaa ctttgtatcc cgaaaaatat atttacgata
     3721 ttcctgggta tccagcaata aaagcaagtg aattaatcga tcaattagaa aaacaattaa
     3781 ctacttttaa ccatacattc catttaaaag aagaagttct ctcgcttact cgtgaagacg
     3841 aagttatcga gattactaca aataaaggca tccattattc caaagcagtc attttagctt
     3901 taggaaatgg ttcattccag ccaagaaaat tgaatttaga taatgctgaa tcatttgaaa
     3961 accacggatt ggattatttc gtgaatgatt tgatgaagta tgctggaaaa aaagtcgcta
     4021 ttgctggagg tggtgattct gctatcgatt ggacgttgat gttagagcct attgcaagcg
     4081 aagtttactt gattcatcgt cgcccggaat ttcgtgcgca cgaacatagt gtatcacgct
     4141 tgaaatcttc atctgtcaac ttgcttactc cctatctcat cgatggatta tcaggcaata
     4201 atggggaact gacagatatc cggttaaaaa aagtgaaaag tgacgagacg attgatttga
     4261 tgattgattc tctgatcgtc aattatggat tctcttctaa tttagaacat ctttcttctt
     4321 ggggacttga tagtaccaga aatgccatta ctgtaaaatc agatatgtct acttctattc
     4381 caggcgttta tgcagctggt gacatctgta cctatgaagg aaaagtgaaa ctgattgcta
     4441 ctgggctcgg tgaagcacct actgcagtga acaacgcttt acactatatt aatccaaagg
     4501 aacgaactca gcctggtcac agcacaagct tatacgataa aaatcttcgt cctagttgat
     4561 tttttatatc tctttgcatt tcaagttaaa cccgaaagca gtttcttgca tttttttcat
     4621 gttccgttaa catgaaatag agaaagaaaa aataaagggg gagcaataat atggtcgtca
     4681 aaactgagac tttagaagaa aaggcaagag aactattgaa ggaacgtggc gtaactgtag
     4741 aagatatcgc acaacttgtc atgtttttac aaaaagacta tatttctgat ttgactttgg
     4801 cggattgtac tgaaagtgtc aacagcgtgc taacaaagcg tgaagtacat aacgccatta
     4861 tcactggaat ccaattagat atattagcag aaaagaaaaa attgatgagt ccgttgcaac
     4921 atattatcga agatgacgaa ggcctttacg gtatcgatga gatcctagcc ttgtcaatcg
     4981 tgaatgttta cggtacgatc ggctttacga attatggtta tatcgacaag gtgaaacctg
     5041 gtattttaaa agaactaaac agccacgatg gcgttcatgt ccataccttc ttagatgata
     5101 tcgtcggagc gatcgccgct gctgctgcca gtcgattagc acacaaagaa ccagaaaaaa
     5161 gaagtcgcct tactcaataa ttcaacaaaa aaagaaagaa aacgtctatt tagacgagtt
     5221 ctttcttttt tgtttgtttc aagctatttt ttcctaagaa aagaaaaaca gcaaaaaata
     5281 attctaataa tacaaaaaat aagataaagc tatgcatgaa aaattcttct ggaagtacat
     5341 tgatgattgg atcagtaacg ggatcgaata accagtcatc gttgttgaaa aacgtttcat
     5401 gaaaaaggat aaaaaagcga tcaaatccta tcatcatgaa aaaaccgaaa acaactggca
     5461 gtaacatgcc gatttgaaac ggacgaatca accgccaaag ccgatcattc ttttgcaaat
     5521 atttgataaa caaaatactc gggatcaaag tgatgaaaaa cacagcataa tccactaaaa
     5581 acaaaatttt cacttcataa aagtgatggg ctccgctagc agaaactggg aaatctggca
     5641 agctgaggat ggttttgaag ggattattga gataattcat taggtgatca aaatttttca
     5701 gtaacgtctc ttgatctaaa gaagtataat ccaaaatttg caaatgtccg atatcaaata
     5761 cgtatagtgg tcgaaagttg atcgtcacac caactgccag actgatcagt gtcagaaata
     5821 ggctgaacaa tccagcatat tccatccact gccatctcga atttttcata ttagaagctc
     5881 cattcatcca gtgattgttt cacataggta gctgggatag gcaacccagg tacatcttct
     5941 tcttttgtaa aacctgataa aacgagcaaa gtatctatat tattgcgtat cccagcttgg
     6001 atatctgttt catagttatc tccaaccatg attacttctt ctttttgcag ccctaatact
     6061 tcaacggctt tatccataat gatggcttct ggttttccga tgtagatcgg cggcgtttgg
     6121 gttgctgtct ctacaaaaga gatgactgac cctgctccag gaagaaggcc gcgttcagta
     6181 ggtatattct tatctgggtt cgttccaatg aaagtagcac ctttttgaat agcaagtgtt
     6241 gctttaacta ctttttcata ggtcagataa ttatctaagc ctaccactac atagtcagga
     6301 gcctcttctt cccatgtaaa tccggcagct aaaatcaagt caatcaatcc tgcttctccg
     6361 ataacataga cttttttccc ttttccatct gctttcataa aatcgattgt tgccaaagta
     6421 gctgtgtaga ctgtctctgg agctacatga atgtcaaact catctgctag acgattagct
     6481 actgtttctg gtgacttagt cgtgttattg gtaacaaaca agaaaggtaa ttttcttttt
     6541 tgcaattctt ccacaaatcg tttaccggcc ggtatgggtt gtgtcccacg ataaatcgtt
     6601 ccgtctaaat caattaaata tcctttatag tccattcatc cactactctt ccctttttct
     6661 gatcgtaaat gctgtttttg ttttttctac ttgtttaggt tgttcttgtt tttttacttt
     6721 ttcagaggtt tttgctgact ctgtttttcg atttttaata accggttttt tgcgattaga
     6781 tgcgactggt ccttttttct catcgatata cgcacgatta tggttttttt tcttttttcg
     6841 gcgctgagtt gtcttttctt ttttaccgcc gattctctcg atgacaaaat acgcacaacc
     6901 aaaattgcaa tattcgtaaa gatagtcttc caaggaatcg attcgctgat ctggatgcgc
     6961 ccgtcgatta tccgcttgga aaaatccttt taggcgtagt tgttcataac cccaatctcc
     7021 tacgatataa tcatatcggg ctagtacatc actgtaacgt tctccaagct tttctgcatt
     7081 aaatccttca cgataatcgg acacaagccg ataatgtctt tcacctatca aaaaattact
     7141 gtcatctaac agtgtcacta gctcgccttt gactttttct gctttaggct cctcaacgat
     7201 ttcttccaaa gcagctgtca gttcttcggt gactgttgtt tcctgttcag ctgatgattt
     7261 tttcttagca atttctgttt tatgctcaac gttctgctgt tcattttgtt ttgcagacat
     7321 gcgttcacca cctatctttt ttattatacc ttgtttttat ttgattgggt agtgagcttt
     7381 caagtaatct cctaccacac ttcggataaa ttcagggaac aaaaattcat tttttccagc
     7441 aatctcaatc gttggaaaaa atggaacaaa catcaaatga tctacggtag taaaagtata
     7501 atttttattc agatcgattg gcttgttctt ccaaaagact tgatggttaa tagaatcata
     7561 tgtgattcca ttatagacca tctgcccaaa aatcttaccg cgaaatccca ttcccttcat
     7621 aggatagtta cgtaaaaagt tacgattttt ttctatttct aaaactaagc ggatcagatc
     7681 atttccaccc aatgtgacat ttaacaagtg catcggatgc ggcaacgcag tgtgaagctg
     7741 atcctgattg acccgccctt ttggaatttc agttaaaaac agaccactgt ttaatattgc
     7801 tgcttccgtc tttcctcgtt ctttcaccgc ctcaaaggct acttggataa acgaatgctc
     7861 attgaagata tctagactca gagaaaatgg caaatctgct acttcttttt cttttaataa
     7921 ttcatgccca cgttctagat aactatttat ttcttgttca tcttctgtaa aagctgtcat
     7981 tgtttcggta gggatggctt tggctgaatg cttaaggatt tttttctctt catcgatggt
     8041 taaatggact tcaccgacat actgcccgta ctttccagcg gctgctattt ggacatcatt
     8101 gattacgtga ccttctctga ataaatgatg ggtgtgcgat cctaaaataa catcaattgc
     8161 tggtatttct tttgcaattt ggaaatcatc ctgaatcccg agatgactca tcaaaactaa
     8221 aacatccact tgtgaacgta aatcttcgac taattctggc aaaatatcat aaggattccg
     8281 tatgtcccag ccattcggat tataagtaag cggaaatgga gcagtaaatg caattagtcc
     8341 gattttcgtc tggtgtttcg ttgtaatgat tttatatggt ttcgtccatg ccggtggctg
     8401 gagcgtattt ttatcgaata agttatccag gatgatatca aactcagcat gatcgtataa
     8461 atgattcaac tcttctttgg aatttcctac gccttcattg tttcctatcg tcgctgcatc
     8521 ataaccaact tcattcatca gcaagatatt ttcctgtcca ttcgttgctt ctgacaatgg
     8581 atgccaacga tccacaaaat cacctagatc tactgcaatc gttgtcgttt tttctttctt
     8641 ttcgttttct cgtttacgtt gatccaaaaa ccttctgatc cttggccaat tttctaaatg
     8701 agaatgcaga tcattcgtat gtaaaatcac tattttttcc atgtaccctc tcgcctctct
     8761 ttatctttat cataccatag gcagcttttt cttccattga aacaagctct atttcagagt
     8821 ttttcctgcc ttgaagcaaa ttgtataagt acaatattcg ataaatcagt ctggctaaaa
     8881 aatcaggaac attcattttt acagtactat aaaaataaaa aaaacagacc cgaaagtctg
     8941 ttgtcgtttc attataatca gacacatggc ggaacctgct tagtgctgct tccttccaga
     9001 cctgacacgc ttcacaagcc caccattgtc ctagcctttc ggcaaagaat agtatacctt
     9061 atttttcatt cgttgaccag tcttttttta atttcttttt tcgctggcgg agctttcgga
     9121 aaaagtcagt caaaatcatc ccacattcgt cttctaaaat gccaccttct acatatgctt
     9181 ggtggttaaa ccgctcgtcc tccaaaagat tcatcaaggt accggctgtt ccgccttttg
     9241 gatcatacgc gccgaaataa acttctgcta cacgagacaa aatcatcgcg ccgctgcaca
     9301 taggacaagg ctctaaggta acaaaaagtg cagcatcttc caagcgccag cttcccactt
     9361 tttcacaggc ttccttgatt gcaagcattt ctgcatgtgt agtagcatct tgcgtggttt
     9421 ctctaaggtt atatcctctt ccaatcactt ttccatccaa tacaaccact gctccaatcg
     9481 gaacttcatg aagcatttct gctttctttg cttctgccaa tgcaaggcgc atccattttt
     9541 ctttttctac tgcatcaaga tttgtattta ccaattaccc ttccctgcct ttgttttttc
     9601 ttgttttact ttagcatatg ataaaaaggc tgtaactaac ttttttttct atggtaaaat
     9661 aaatacaaga ggtgaacaag atgaaaatgg ataaattact tgctctttac ccacaagcac
     9721 agaaaaaaaa ctctccagca tcagaagcgg atatcctttc ttttgcaatt gatgatgctt
     9781 ttatctggat caaacaagat tcattatctc aacaagaaac cagccttttg aaagcgttat
     9841 tccctgtaat caacgatcaa aaaaaacacc catggtatcg ctatcttttt aaagatgctt
     9901 cttgtgttca agaaaaaagc tttcgtttga tccagcttca cgtcgaatca aaaggagaat
     9961 tcttaaaaaa agaatggcaa gatactattt tggaattatt tcgctattct gctgatttct
    10021 ttttttatac agacacagat gcgctcttag tcgaacaaat aaaccgtgat tatcttgatg
    10081 ctactgaaat caacggtatt tttctatctt tagatgctga ttttgatacg acaacgtctg
    10141 tattcgttgg ttctttccat tcacctggca ccgacctcgt ccctttgttt gctgaagaaa
    10201 gaaaaatttt tttaacagag caaaacaatc tttatactca cagccgtact ttttctatgc
    10261 aagatgtggc tttacattac tatacaaaag atactgtaaa agatagtaag ttgatcaaaa
    10321 gttacataga actgatcaaa caaaatgaaa tggaagtgat cattcaagaa ttatggaatg
    10381 aattaggcaa tgtcagctct gctgcaaaaa cactgtttct tcatcgtaat actctaaaat
    10441 atcgaatcga aaaatttcaa gaacagtctg gtttcaatct aaaaaaagcc aacgatttac
    10501 tgttttgcta tcttttattg cttcatgaac ataattaaag atagattaaa ggggttgtga
    10561 caataatttt gtcacaaccc cttatttcga atgaatagtg ttcaaaagct agtttctctt
    10621 acatcatttt ttcaacaaaa tgcattagcc ggtcttttac atcttcggtt gaatctttga
    10681 ttgaatcacc ataaacagag attttctttt ttcctgattt gatttttgct aacattccca
    10741 tcaaagaatc caagtcacta tctgaaagac catctacgat ttctagtaat ttttcatttc
    10801 catcaaattt gtcatagacg aattttttta ctttcgtccg agtagtcata tgatggatct
    10861 tttccattac ttttcccgat acaacgactg ctgtcgctac actggcagcg gctgctacgc
    10921 ttaatccgat tgttactttt gttgatgttt tcataagtat cagctccttt ttatctatct
    10981 ccatcatagc atagacaaaa aaataaacga actaatccac tcagtgaaat ctcaatcctt
    11041 tagggaaaaa gttctttact gttcctttta cttgtttccc aaatccgaca ggtatcttat
    11101 ctaatactag caaaacccat ccttgattcc catcacgttg aaatgtttct cctgctgtgt
    11161 aagattgcca ttccttttga gtgatcggca agcaaggtat attctctatc ttttttgtag
    11221 caagcgctaa tgcatagcta ggttcaaatc gattcttttt gaaatctcct aaatgcaaac
    11281 ctgtacgtac gacttttaaa ccatcgagag aaggggctaa ctcaggaact tcccaaagat
    11341 ggtcgttgaa cactaaaagt cttccagtag cttcataatg aaaatcattt gaaaactccg
    11401 tccagagttt ttcctgctct tttgtcattt gcactttact tttttttcgt gtcttctttt
    11461 ctttatgcat ctgattttgt ccatgaaaag tcagctttgc aacaaaatgt ccttcaccct
    11521 gatctttatg aggccatata cgtattgttt tttccaaacc agctacgctt ccccattcag
    11581 aacgtcctga actaacggat tgagtcaaag gaatctcctc aatcgttact ggataatttt
    11641 ctacaagcca gctaatgatc tcttcatttt cttctggtgc aaacgtacaa gtcgaataaa
    11701 tcagttgtcc tttatttttt agcattttta ttgctgaaga caaaatctct tgctgacgtt
    11761 tttgacagta tagcggagat tcttctgtcc actcttttat tgcatttgga tcttttcgga
    11821 acattccttc tcctgaacaa ggagcatcaa ccacgatccg atcaaaaaag cccgaaaaat
    11881 gagggacaag ttctgcgggt gcatgatttg tcactattgc gttcgaaact ccccatcgtt
    11941 ctatattttc tgataaaatc ttcgcacgtt tcggaaagat ctcattcgtc actaataacc
    12001 ctttcccctt catttgagca gctaattggg ttgatttacc accgggagcc gcacacaaat
    12061 ccaaaacttt ttcaccgggt tttgctgcag cagctgttcc aacgatcatt gcacttggtt
    12121 cttggctata ttcataccca gcttgatgga ggaaagactt tccattgaca gtgccaagaa
    12181 atccctcatt gctgtaggga gcgggttgta attcttcgct atggtaagtt tggaccatgt
    12241 ccaatcctgc cggttttagt ggattccacc gaaatccttt tttcacgctt ccttgttcaa
    12301 gcgcagaaaa aaaatcggat gcttcttcgc ctaaaagaag acgatatttt ttgataaatt
    12361 gctgtggtaa cgttgtcgct tcctccttca ttccaatgtt tcgttagaca ttttacaatc
    12421 ttttttacga aaaataaagg tggctgatct actttttcat gttgctgcct aaggtgactg
    12481 ttcctttaag atattttcca agtattcatc cgtcacctcg tttttatccc aaacagcgtt
    12541 catcatttca agaaaatctt gagaagaatc atctgagcgc aaacttccca atgcattaag
    12601 ccataccaat tcaagcgact tttcttcttt gatcttcgtg tgacctttga catatgagaa
    12661 aaatggccgc ttaaatagcg aatccatgtc taaatcagat tttgcaatga tttcacgaat
    12721 actttcatgg atagaaaaaa aatctttcaa taagtagaca atgcttaaaa catcatcgga
    12781 actgcattca gctaagtagt tttcaaaata aacagtgatt tcttctaata tcttctcttt
    12841 taacccgctg attgatttga aatgtgtata aatcgggtat gtagaacaac cgcagaactc
    12901 tgctaactct cttgcagtaa ttgacttcat tccatattct tccacgtata ccatactttt
    12961 gaacagcaaa tattctttac tataatttgt tactctcccc atcattctta cctccgtctc
    13021 ttttcatcta cccataaata aataacataa ataattctaa aaatattatt gataattatc
    13081 aaaacatatt tagttatatc cattaaaaat caatttttcc gccttaaaaa caagaatatc
    13141 ttgacaaaat accctaggaa ataaacaata gaataaacaa ggtaacaaaa aaattaaata
    13201 acgttatata aaaaagaagg cgacactctg tgagaataac acagactgaa atgactttcc
    13261 actcacagtt tttgtctaac tatatacaaa aacagaataa aaaggaggta tttatgctaa
    13321 ttgaagcaag aaaaaaagag ttacttgttc tacaaatggt cattcaaaaa aataattgtt
    13381 ctttacaaga attatccaat gacttacata ttccaaaacg gacaatcaaa gaactgatcc
    13441 gaaaaataaa tgtatcactc aagcaatttt tattgattga tgaatttatt tattctaatc
    13501 ataaaggaga gatccaaacc aaagaaaccg atcaaactaa aaagttagct gttttttcta
    13561 agttaaaatt atgctaccta aaagaatcca atcgttttaa ctatttgatg ttactaatca
    13621 attatccaca aactgctgta ccaaaaaaat atttacttga acaactttat atatcgccct
    13681 cctatctcga aaaactgaca aaacagctca atcattcctt aaaaaaattc catttacgga
    13741 tcctatctgt aaaaaattgc tgtactcttg aaggaaatga gttatctatc cgtctctatc
    13801 tttacttttt tctttcagat acatttggag gtaacgattg gccattttcc tcacttgaat
    13861 taaatgcact aaaaatgaac agtacagatg agcaaataga aaaaatttca aaacaagaaa
    13921 aaaatatcta cttgctgtta actttattca tattacgaaa caaacaacag gcatattttc
    13981 caaaaacttc ttctgaaatc actgaattga tggacttatt gcagcataca catgattatt
    14041 cagaatactt tacttctttt tcagtgagtc gcccttgtga agagacagaa acagagagag
    14101 cctactttaa ttattttttc tattgttttt ttccaaatca aatctctttt cgacaaaaac
    14161 aggagattgg ttttttattt tccacaagca aaaaccttat ttgtcgacaa gtcggtcaat
    14221 tgatgactta cttaactcaa cagtttcctc tcaaccattt agaaaataaa ataccaattt
    14281 atttttattt tcttgtgttg gcagttgttt ttttcgaact gttgggtgaa tccagcagta
    14341 gtttttctac actttgcttt ccagaagcca cgtccaatca ttctagttct tttatacagg
    14401 aaaagaaatt aagagaaaaa atcgatacaa ttttttcaaa aaatggggaa agaaaccaaa
    14461 tcagtcaata ccttagtcaa ttgatccaac acttactaca aacagaaacg aaagagcagt
    14521 tactcatcca tttgcagata accaaacagt ttacaggaaa ttatcatctt caaagccatt
    14581 tgcagcagtt atttcgcaaa gatgctgtta aaattaccgc atattcagaa catgctgatt
    14641 tgatcattac agatcagcat gagaaaattt cagatgaaac ggagactttc tatctggata
    14701 caacgcacaa tacagtgtta tttggcaagt taataaaagc ggttcatgaa ctgtatatga
    14761 aaaaaggtta tgagaacaaa acaaagagga tgtgacaaaa ttcttgtcac atcctcttat
    14821 t
//
LOCUS       DASHRQ010000067        14624 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.071, whole genome shotgun sequence.
ACCESSION   DASHRQ010000067 DASHRQ010000000
VERSION     DASHRQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.071"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            3..815
                     /locus_tag="U2M88_002244"
     CDS             3..815
                     /locus_tag="U2M88_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEM8374598.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            812..1612
                     /locus_tag="U2M88_002245"
     CDS             812..1612
                     /locus_tag="U2M88_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEM8374599.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1773..3041
                     /gene="rseP"
                     /locus_tag="U2M88_002246"
     CDS             1773..3041
                     /gene="rseP"
                     /locus_tag="U2M88_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEM8374600.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3109..4818
                     /locus_tag="U2M88_002247"
     CDS             3109..4818
                     /locus_tag="U2M88_002247"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEM8374601.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5030..9388
                     /locus_tag="U2M88_002248"
     CDS             5030..9388
                     /locus_tag="U2M88_002248"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEM8374602.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9533..10006
                     /gene="rimP"
                     /locus_tag="U2M88_002249"
     CDS             9533..10006
                     /gene="rimP"
                     /locus_tag="U2M88_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEM8374603.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10029..11204
                     /gene="nusA"
                     /locus_tag="U2M88_002250"
     CDS             10029..11204
                     /gene="nusA"
                     /locus_tag="U2M88_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEM8374604.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11226..11519
                     /locus_tag="U2M88_002251"
     CDS             11226..11519
                     /locus_tag="U2M88_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEM8374605.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11516..11827
                     /locus_tag="U2M88_002252"
     CDS             11516..11827
                     /locus_tag="U2M88_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEM8374606.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11840..14146
                     /gene="infB"
                     /locus_tag="U2M88_002253"
     CDS             11840..14146
                     /gene="infB"
                     /locus_tag="U2M88_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEM8374607.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14173..14520
                     /gene="rbfA"
                     /locus_tag="U2M88_002254"
     CDS             14173..14520
                     /gene="rbfA"
                     /locus_tag="U2M88_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEM8374608.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4982 a         2598 c         3255 g         3789 t
ORIGIN      
        1 gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat
       61 ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt
      121 gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa
      181 agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt
      241 cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg
      301 acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg
      361 gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa
      421 caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagt agagcagaga
      481 tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag
      541 aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac
      601 aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat
      661 ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg
      721 cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa
      781 aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtgatca cagcagctgt
      841 ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc
      901 agcaatctta gcggtagtag gagtttacga gctgttccgg atgaaaggat tgacactctt
      961 aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag
     1021 atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat
     1081 gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt
     1141 tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac
     1201 agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc
     1261 atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa
     1321 aacgattgaa ggtgcgctcg gtggtatcgg tagtgctgtt gtagtcgcat ttctttattt
     1381 attgctatac ccagcaaaag aactattcaa tcatggtatg tttgtcatgc ttctgatgac
     1441 gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca
     1501 ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt
     1561 cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata
     1621 cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata
     1681 ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa
     1741 aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg
     1801 tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag
     1861 gaatcctcgt ccgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa
     1921 aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag
     1981 gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt
     2041 ctgacggcat cgtagagaag ataaatttaa gtaagaaaat ccaattgaca aatgctatcc
     2101 cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg
     2161 gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg
     2221 acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc
     2281 agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca atcgtgttat
     2341 tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag
     2401 agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca
     2461 gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc
     2521 caggtaaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga
     2581 caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa
     2641 tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat
     2701 tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg
     2761 gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta
     2821 ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg
     2881 ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa
     2941 gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg
     3001 tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg
     3061 tataataaca aacgataact attttgagga aataaaggag tatatagaat gagacaatca
     3121 aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa
     3181 atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca
     3241 ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc
     3301 ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg
     3361 cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg
     3421 atccttggac caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca
     3481 tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga
     3541 cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcata ttctttccat
     3601 gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta taccgaagta
     3661 ttcaagcgtt gtggcttaga atttcgctcg atcatcggag acggcggcgc aatgggcggg
     3721 aaagactcaa aggaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg
     3781 actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa
     3841 tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac
     3901 gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt
     3961 gcggatgaac aacctgtcct tgttttggta cgtggagacc atgaagtcaa tgacgtaaaa
     4021 ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa
     4081 tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta
     4141 tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga
     4201 ttccactata aaaatgtgaa tccagaacgc gatttcactg tgctttctta tgaagatctt
     4261 cgttttgtac aagaaggaga tccttcacca gacaacaacg gtgttcttgc ctttactcga
     4321 ggaatcgaaa tcggacatat attcaaaatc ggcactcgtt acagtgaatc tatgggagca
     4381 actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt
     4441 gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaattgg
     4501 ccaaaaggaa ttgcgccatt tgatctgcat atcgtccaaa tgaatctaaa agacgaatac
     4561 caaacaagcc tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg
     4621 gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt
     4681 ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag
     4741 aaaactggcg aaatggttga agtacgtaaa gaagaattgg cgaatacttt accaatcctc
     4801 ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg
     4861 tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca
     4921 attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg
     4981 ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa
     5041 acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca
     5101 tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg
     5161 ggaatttacg ctacattttt ccaaaatttt gcctattatg ctatatcggt cattgactca
     5221 gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga
     5281 ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca
     5341 atgcgataca ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg
     5401 taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt
     5461 gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga
     5521 agtggatgag cagcaagcgg aacgagtatt gaaacttttt gaagaacgca aacaagaaca
     5581 ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa
     5641 ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cacatccagc taggacgaaa
     5701 tattccagcc gatgaaccaa cgacaccaat gatcagtatc gttgaagaag aacgaagagt
     5761 cacacttgag gggtacgtat tcgacaaaga agtcagagaa ctacgttcaa aacgcaagat
     5821 cttgactttg aaaatcacag attatacttc atcatttatc gtcaaaaaat tctcgaacgg
     5881 cgaaaaagat gaacaagtat ttgatgcgat cagtgtcggt agctggttaa aggtacgagg
     5941 aagtattcaa gaagatacgt ttgtccgtga tttggtcatg aatgcgcaag atatcataga
     6001 agtcaaacat acacctcgca aagattatgc tcctgaaggg gaaaaacgag tagagcttca
     6061 tgttcatagc aatatgagta cgatggatgc aacaaacagc atctctgatc ttgtggcaca
     6121 agcagggaaa tggggacacc gagctatcgc cattacagac cacggtggtg cgcaagcctt
     6181 tccggaagca catagtgctg gaaaaaaagc aggtgtgaaa atcttatacg gcgtggaagc
     6241 gaatgtcgta gatgatggtg tcccaattgc ttataatgat gcacatgaag cattgagtga
     6301 agccacatat gtcgtatttg acgtggaaac gacaggattg tctgcagtat atgacacgat
     6361 catcgagcta gccgcagtca aaatgtataa aggaaatgtg atcgaaagct ttgatgaatt
     6421 tatcgatcca ggacatcctt tgtcacgaac aacggtagat ctgactggta tcacggatgg
     6481 aatggttcga ggatcaaagt cggaagaaga agtattgcgg atgttccttg aattttcaaa
     6541 agatacaatt ttggtagccc acaacgcggc ttttgatatg gggttcttga atactagcta
     6601 cgcaaggtat ggtattcctg aagcggccaa tccagtcatt gatacgttgg aattagctag
     6661 atatctgtat cctcaattca aacgtttcgg attaggtgtg ttatctaaaa aatttggtgt
     6721 cagcttggaa cagcatcacc gagcaatcta tgatgctgaa gcaactggtc acttagcatg
     6781 gatctttgtg aaagaggcaa tggacaatca taacatgctt tatcatgatc agctaaatga
     6841 gcatatcgga gaaggagatt cttacaaaag ggctcgacct ttccatgtaa cgattttagc
     6901 aaaaaatcag gcaggactga aagatttgtt caaactgatt tcaatgtcga atgtcgaata
     6961 cttcgaacgc gtcccacgaa taccacgctc ccaattgaaa aagatgcgtg aaaatctatt
     7021 gatcggctca gcttgtgata agggagaaat attcgaagcc atgatgcaaa aaggggtaga
     7081 agaggcaaga aaccgagcaa aattctacga ttatattgaa gttatgccaa aagcggtcta
     7141 tgcgcctttg atcgagcaag aattggtaaa aaatgaacat gatttggaag aaattatcca
     7201 aaatttggta gaaatcggaa aaagtttgga taaaatcgtt gttgcaacag gtaatgtaca
     7261 ttatctgaac gaagaagatg ccatttaccg aaaaattttg atcaattcta tgggcggagc
     7321 gaatccgttg aatcgtcata gtttgccgga tgtccatttc cgaacaaccg atgagatgct
     7381 aactgcattt cactttttag gggaagaaac agcgaaagag atcgtagtag aaaatacgaa
     7441 taaaatcgca gatatctgcg aagaagtgat tccagtcaaa gatgaacttt atacaccgaa
     7501 gattccaggt tcagaagatg aaatatcaga attgagttac acaaaagcaa aacaaatgta
     7561 cggagatccg ttacctgaga tcattcaaaa acgattaaaa aaagagttga attctattaa
     7621 tggtaatggc ttctcggtta tttatctgat cgcccaaaaa ctagtgcata aaagtaatga
     7681 agatggctat ctagtaggtt cacgtggctc tgttggctct agctttgttg cgacgatgac
     7741 aggaatcact gaagttaatc cgttagcccc tcattattat tgtccagagt gccaatattc
     7801 cgagtttttt gaagatggta cgtatggttc tggatttgac atgccagaaa aacaatgtcc
     7861 aaaatgtggt gcacgcttaa acaaagacgg tcatgatatt ccatttgaga catttttagg
     7921 attccatgga gacaaagtgc ccgatattga tttgaacttc tcaggtgatt atcaagcaga
     7981 agcccataac tatacgaaag tattgtttgg cgaagactat gtttaccgtg ccggaacgat
     8041 tggtacagtc gccgacaaaa cagcatatgg ctatgtaaaa ggatacgaaa gggacaataa
     8101 tctgcagttt cgaagtgcag aagtcgacag attagcaaaa ggcgctactg gagtcaaacg
     8161 gacaaccggc cagcatccag gggggatcat cgttattccc gattatatgg atgtctatga
     8221 ttttacgccg atccaatatc cagcagacga tcaaaattcg gaatggaaaa cgactcattt
     8281 tgattttcac tcgattcacg ataatgtatt gaaattagat attctagggc atgatgaccc
     8341 aactgttatt cggatgcttc aagatttatc agggatcgat ccacaaacga tccctacgga
     8401 tgacccggaa gtgatgcgga tatttgccgg tcctgaagta cttggagtaa gtcaggaaca
     8461 aatttattcc aaaacaggga cattaggtat tccagaattc ggcactcgtt ttgttcgagg
     8521 gatgctagaa gaaacgcatc ctacaacttt tgcagaacta ctgcaaattt ccggactatc
     8581 tcatggtaca gacgtatggt taggaaatgc ggaagaattg attcgacgag gagatgcgac
     8641 actggcggaa gtaatcggct gtcgtgatga tattatggtt tatttgatcc atgctggatt
     8701 agatagcggg atggcattta aaatcatgga aactgttcgt aaaggacaat ggaacaagat
     8761 ccctgatgaa ttgcgagaaa cctatctatc ggcgatgaaa gaaaataatg tgcccgactg
     8821 gtatatcgat tcttgttcaa aaatcaaata tatgtttccg aaagcccatg ctgcagctta
     8881 cgtattgatg gctcttcgtg tcgcttattt caaggtttat ttccctattt tgtattactg
     8941 tgcatacttt tctgtacgtg cagacgactt tgatctagtc tccatgtgca aaggaaaaga
     9001 tgctgtgaaa caagcaatga aagaaatcac ggacaaagga ttagatgctt ccgtaaaaga
     9061 aaagaatcaa ctgacagtgc tggagttagc caatgaaatg ctggaacgag gattcaaatt
     9121 tggaatgatt gatttgtata aatcggatgc cgttaacttt gttattgaag gtgatacgtt
     9181 gatcgcacca tttagagctg tcccaagttt gggaaccaac gtggcaaaac aaatcgttga
     9241 agcaagaaaa gacggtccat ttttatccaa agaagatctt gccacccgcg gtaaagtttc
     9301 aaaaacattg atcgaatata tgaacgacaa tggcgtattg aaagatctac cagatgaaaa
     9361 tcaattatcg ttattcgata tgctataagc atctgttttc ctgtctgctt gtaattttaa
     9421 gcagagtatg gtatagttct tataggtaat gatactaact gtgagtgagc ggatttttcg
     9481 ctcactcttt ttaatggaat tacgcactta attttttaag gaggcgaaac ttttgagtag
     9541 cgtcgttgaa acagtcacag aaatggtgac accgatttta gatgaacaaa attttgaact
     9601 tgtcgaagtc gaatttgtaa aagaaggaaa aagctggttc ttacgagtat ttattgacaa
     9661 agaaggcggt atcgatatcg aagaatgcgc ctttgtcagt gagaaactga gtgaaaaact
     9721 tgatgcagca gatccagatc cgattcctca agcttatttc ctggaggtat cctccccggg
     9781 agcagagcgg ccattgaaaa aagaaagcga ctatgaaaag gcacttggtg attatgtcca
     9841 tgtctcctta taccaaccag tagatggcga aaaacaatat gaaggatttc ttcaatcatt
     9901 tgatgctgaa cagcttacat tgaaaatacg cataaaaaca cgagaaaaag aattgacttt
     9961 tgaccgaaaa aatattgcaa aagctcgctt agctatccaa ttttaatcac ggagaggaat
    10021 cagaaaaaat gagtaaagaa atgttaaatg cgttagacgc tttagaagct gaaaaaggga
    10081 tctcaaaaga aatcgtgatc gatgctttag aagcagcatt ggtttctgca tacaaacgtc
    10141 actatggcca agcacaaaac gtcgaagtgg aatttgatca gaaaaaagga aagatccatg
    10201 tctatgcggt aaaagaagta acggaagaag tgatggattc acagttggaa gtctcattga
    10261 aagatgcact tttaatcaat ccagcatatg agatcggtga tacgatccgt tttgaagtaa
    10321 caccaaaaga ttttgggcgg atcgctgcgc aaacggctaa acaagttatt ttacagcgtg
    10381 tacgtgaagc agaaagaacg attatttata acgaattcag tgcttatgaa aaagacatca
    10441 tgcaaggaat cgtcgaacgc caggataaac gttatattta tgtcaatctt ggtaaaattg
    10501 aggcagttct ttctaaacaa gatcaaatgc caaatgaatt ttatcaacca catgatcgaa
    10561 tcaaagttta tgtttcccga gtagaaaata cgtctaaagg tccgcaagta tttgttagcc
    10621 gcagccatcc agacctattg cgccgcttat ttgagcaaga agtaccagaa gtctatgatg
    10681 gaatcgttga aattgtcagc atcgcccgtg aagctggcga tcgttcaaaa gtggcagttc
    10741 gctcaaatga tccaaatatc gatgcagtcg gtacatgtgt cgggccaaag ggacaacgag
    10801 ttcaagcaat cgtcaatgaa ttaaaaggcg aaaacatgga tatcgtcgaa tggaatgaag
    10861 atccagctgt attcattgca aatgcattga atcctgcaca agtcatggat gtgatcttcg
    10921 atgagacaaa tccaaaggcc tgcacagttg tcgttccaga ctaccaatta tcgctagcaa
    10981 tcggtaaacg cggacaaaac gcacgtttag ctgctaaatt gactaaccat aaaatcgata
    11041 tcaaatcaga atctgacatg acggaatttt atgaaaatca agaacaacaa aaagaaacag
    11101 aagaactgca tgatgaagca atcgttcagt cagatttaac agacgatgaa tatgaaacaa
    11161 ttgcattcaa caatgaaacg gtcgaagaaa aaccagaagc ttaactttag taaggaggca
    11221 aagagatgaa acaaaggaaa attcctttac gcaaatccgt ggtttcaggt gaaatgaaac
    11281 ctaaaaaaga gatggtccga atcacccgtt caaaagaagg ggaagtatcg atcgatccaa
    11341 ctggaaaaat gcctggacga ggagcgtatg tctcacttga gccggaagaa gtacaacaag
    11401 cttgggataa aaaaattctc gatcgagtac tcgaaacaac attaacagat gagttttatc
    11461 aagaacttct tgactatgtc actcaccaaa aagcacgtaa agagttattt ggaaaatgaa
    11521 tacagttaat cggcaaaaag ccatgaatct catcggtcta gcgatgcgcg cgggaaaact
    11581 aatcacgggt gaagaactga ccattgctga aatccgcaaa caaaaagcaa aattggtgtt
    11641 tgtggcaacc gatgcaagcg agaatacaag aaaaaagata aaagataaga gttcatatta
    11701 tgaagttcct tgctttgagc tattttctga agcagaaatc acgcagatga tcggaaaacc
    11761 tcgcaaggta ttcggaataa tggatgccgg atttgcgaag aagaccaagg agctaattga
    11821 aggttaggaa ggtgattgca tgggcaacaa aagaatctat gaattagcaa aagaactcaa
    11881 aaaatctagt aaagacgtcg tggaaaaagc acaacaactt ggcatcgatg tgaaaaacca
    11941 tatgggtgcg attactagca atgatgagaa gaaattgcaa caagcgttca aaaacccaag
    12001 cacgaaacaa caagctgcac aacaagcaag cagaaaacca gccaatcaac agccgaacga
    12061 tcaacgtaaa aaagaaacaa cgaacaatca acaaaaaaat aaaaataacc gcagttatca
    12121 agatcgcggt caagggagcg gtcaagtgaa tcaaggaaaa aaaccaacaa caaaccaaaa
    12181 taatacggga aataccagca gccaaaatcg tcaaggaacg agccaaggcg gcaatcaaaa
    12241 ccgtactaac aataacaaca aaccaaacaa taacaatcgc ggcaaattta ataataataa
    12301 ccgtaatcgt tataataaaa aaggtaaaaa aggcaaacag caacaatcaa ctaaaccagc
    12361 tgtaccacca cgcaaattcc gtgaattgcc agatgtatta gagtatactg aaggtatgaa
    12421 cgtagcagat attgcgaaaa aaattcatcg tgagccagca gaaatcatta aaaaattatt
    12481 tatgcttggt gtcatggtca atcaaaacca agcactggat aaagatacga ttgaactgtt
    12541 agcgacggac tatggcatgg aaccacaaga aaaagtccaa gttgacattg ctgatatcga
    12601 taaattcttc gaggcagaag aagtcgatcc agataaatta gtctcacgtc cgccagtcgt
    12661 aacgattatg ggacacgtag accatgggaa aacaacttta ttggatacat tacgccattc
    12721 acgtgtaaca agcggcgaag ccggcggaat cacacagcat atcggtgcct accagatcga
    12781 tattgatggt aaaccaatca ctttcttgga tacaccagga catgcggcct ttacaagtat
    12841 gcgtgcacgt ggagcaagta tcacagatat cacgatctta gttgttgctg cagatgatgg
    12901 cgtcatgcca caaacagttg aagcaatcaa ccatgccaaa gctgcaggcg ttccaatcat
    12961 cgtcgctgta aataaaattg ataaaccagg tgcaaaccct caacatgtta tgcaagaatt
    13021 aagtgagtat gagttaattc ctgaagcatg gggtggagaa acgatctttg ttgagatctc
    13081 agcgaaattc ggccaaaaca tcgaagaatt gctggaaatg atcctgttag tcgcagaagt
    13141 agaagattta aaagcagatc caacacaacg cgcaatcgga acagtgatcg aagcacgtct
    13201 tgataaaggt aaaggtcctg tgtcaacctt acttgtccaa gaaggaacac ttcatgttgg
    13261 tgacccaatc gttgtcggta atacttacgg acgtgtccgt gtaatgacaa acgatcttgg
    13321 ccgtcgcgat aaagaagcag gacctgcaac gccagtagaa atcactggct tgaatgatgt
    13381 acctcaagca ggtgaccgtt tcgtagtctt cgaagacgaa aagacagcac gtgcagcggg
    13441 tgaagaacgc gcgaaacgtg cattattaga aaaccgtgct gccacaagcc gtgtgacttt
    13501 ggataactta tttgaaagcc tgaaagaagg cgaattgaaa gaagttaacg taatcatcaa
    13561 agcagatgta caaggttcag cagaagcact ggctgcatca ttgaagaaga ttgatgtgga
    13621 aggtgtccgt gtaaaaatcg tccattcagc tgtcggagca atcaatgaaa gtgatgtaac
    13681 cttagcagca gccagcaacg ctattatcat cggtttcaac gttcgtccta caccgcaggc
    13741 aaaagtgcaa gctgatactg aagaagtcga tattcgtctt caccgtatca tctataaagc
    13801 catcgaagaa atcgaaactg cgatgaaagg gatgctagac cctgaatttg aagaaaaaat
    13861 caccggtcaa atgatcgttc gtgaaacctt caaagtttct aaagttggta cgattgcagg
    13921 tgcctatgta acagaaggat atattcgtag agatagcggt gtccgtgtaa tccgtgacgg
    13981 aatcgtaatc tatgaaggcc aacttgcaag tctgaaacga ttcaaagatg acgtgaaaga
    14041 agtcaaaatg ggttatgaat gtggtgcaat gattgaaaaa ttcaatgaca tcaaagtaga
    14101 tgacgtaatt gaaggattta tcatggaaga agtcaaaaat gactaaaaac tgataaaagg
    14161 aggccaatca ttatggctaa ctaccgtgac cgccgagtcg gtcaagaaat cttaaaagaa
    14221 gtcaatgata ttttgcgcaa aaaggtccgt gaccctcgtg tggagaacgt aacgatcaca
    14281 gatgtgaaag ttacggggga cttacagcaa gcaacgatct attacagcat tttatctgac
    14341 ttagcatcag ataagaaaac agcacaagat ggattgaata aagcatcagg attgattcgt
    14401 cgagagttag ggcacaatct aagtatttat aagacacctg aattgttctt tgaacttgat
    14461 gaatcagttg tctatggcaa ccatatcgat gaattgcttc gcgacttgaa caaagactaa
    14521 tcaaaaactc ccgcaaatcc tgtgaaatca ggctctttgt caataaggac tgatgagttg
    14581 tgcaaaatca aatctgagtc agaatgaacc acattctggc tcag
//
LOCUS       DASHRQ010000068        14154 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.072, whole genome shotgun sequence.
ACCESSION   DASHRQ010000068 DASHRQ010000000
VERSION     DASHRQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.072"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            1..301
                     /locus_tag="U2M88_002255"
                     /pseudo
     CDS             1..301
                     /locus_tag="U2M88_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            432..1382
                     /locus_tag="U2M88_002256"
     CDS             432..1382
                     /locus_tag="U2M88_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEM8374609.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            1900..2355
                     /locus_tag="U2M88_002257"
     CDS             1900..2355
                     /locus_tag="U2M88_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEM8374610.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2696..3298
                     /locus_tag="U2M88_002258"
     CDS             2696..3298
                     /locus_tag="U2M88_002258"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374611.1"
                     /translation="MLDPLFTDETFITFGFGHLVELAEPGHYDEKWQNWKLESLPIFP
                     DRYDFEVAKDKGKQFKIVAELLKKANTIIVATDSDREGENIAWSIIHNANAFSKDKTF
                     KRLWINSLEKDVIRSGFQNLQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQQK
                     GVQGTFSLGRVQTPTLYLIFQRQEAIENFKKEPFFEVEAK"
     gene            3392..3724
                     /locus_tag="U2M88_002259"
     CDS             3392..3724
                     /locus_tag="U2M88_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HEM8374612.1"
                     /translation="MGNQEGIIADVQTKEKKTNSPSSFSLSSLQSKVNQLYKATASQT
                     LKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIRSN
                     NSTQILTN"
     gene            complement(3875..4854)
                     /locus_tag="U2M88_002260"
                     /pseudo
     CDS             complement(3875..4854)
                     /locus_tag="U2M88_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(4859..5626)
                     /locus_tag="U2M88_002261"
     CDS             complement(4859..5626)
                     /locus_tag="U2M88_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEM8374613.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6116..6541
                     /gene="lacA"
                     /locus_tag="U2M88_002262"
     CDS             6116..6541
                     /gene="lacA"
                     /locus_tag="U2M88_002262"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEM8374614.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6558..7073
                     /gene="lacB"
                     /locus_tag="U2M88_002263"
     CDS             6558..7073
                     /gene="lacB"
                     /locus_tag="U2M88_002263"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEM8374615.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7084..8016
                     /gene="lacC"
                     /locus_tag="U2M88_002264"
     CDS             7084..8016
                     /gene="lacC"
                     /locus_tag="U2M88_002264"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEM8374616.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8019..8999
                     /gene="lacD"
                     /locus_tag="U2M88_002265"
     CDS             8019..8999
                     /gene="lacD"
                     /locus_tag="U2M88_002265"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEM8374617.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9027..9344
                     /locus_tag="U2M88_002266"
     CDS             9027..9344
                     /locus_tag="U2M88_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEM8374618.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9344..11050
                     /locus_tag="U2M88_002267"
     CDS             9344..11050
                     /locus_tag="U2M88_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM8374619.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11192..12598
                     /gene="lacG"
                     /locus_tag="U2M88_002268"
     CDS             11192..12598
                     /gene="lacG"
                     /locus_tag="U2M88_002268"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEM8374620.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12621..12893
                     /locus_tag="U2M88_002269"
     CDS             12621..12893
                     /locus_tag="U2M88_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEM8374621.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            12914..13813
                     /locus_tag="U2M88_002270"
     CDS             12914..13813
                     /locus_tag="U2M88_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEM8374622.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4614 a         2349 c         2737 g         4454 t
ORIGIN      
        1 ttttttaccg atgatgatat ttttttaatt ttagcaatac tttttcttgt ttcatctaat
       61 tttagtgata acttaattga agttcttaca gaaccagaaa ttcggtctat gacggatgaa
      121 gaacaacaga ttattaaagc tatgatttcg gaaaataaga aagacgtagt agttatcaaa
      181 aagattagag agtatacaaa tttaggtgtt ttacaagcca aaaaagtata tgaaaaaaaa
      241 aaaaagggtc tataattaca aattagataa tacattagaa gctggagaca agattgtcta
      301 gataatgtgt gagataaaat ttttggcagg ttatcataca taagacaaaa tgtttttaaa
      361 actgataacg tatttattcc acaacaatta cgcatttatt tctgagaaaa atataaagca
      421 ggaggattac aatgctgaaa acttattcct taacaaaaga tacattatta gtttcgtcag
      481 aatcaaatat caaaaattct tctgttgttt atgtttatgg tgacgaacga gaatatatcg
      541 aatcttctga acataaatat gactttaaaa taggcaatat actaacgttt gatgatcgag
      601 ttgcatatga cacgatgata gataagaacg atgaaaaaag tttgctcgta agttttttgt
      661 ttcccgaggt acatgaaggt gggcatctag ataccctgac gacatctgtt gttttcttgg
      721 tatttaaaaa taagttgata atttttacaa aacaaaaatt aaaattcatt cccgaactct
      781 tatccaatat ggttttacgt gatgtcaatc atttcgtgga ggaagtactt ttaaagatca
      841 tgcaaaaaga tttctatatg atgcttaacg accttcgtgg agtcaaggaa aaaattgatg
      901 atttagactc agaaatatca acgtccggtt cactcaaacc aacgtttggt gatttattga
      961 ctcttcaaaa atacatgata gctttgtcaa caacttataa agctaaccat aaagcgcttg
     1021 atttcattaa aaaaaatttc acaactattg acgatattga ttttagtact aaaaaaataa
     1081 ttgatgattt atacgataca agtgatacaa tggatagaat cattttaagt tacagccaat
     1141 atttggacaa attggaaaat atgataaaca atatgatttc gtatcagcta aacatgatta
     1201 tgaaaaccct aaccgagata tcaattgttt tgactattcc agcaattatt tttggctttt
     1261 ggggaatcaa tgtccatgtc ccgtttgaaa agtcttctta tggtgtttta gccgtattga
     1321 ttatctctgc tatactaagt ctaatttgct ggcttttgat gcgacgcaaa acatatttgt
     1381 aaagtatttg tgatacccta atatgattta aagcagaatt tctttgttga ctttttaaaa
     1441 ggctgattcc tttcaataat ttaatagctt cagcggctgc tcagaagtca ataaattgga
     1501 ctatttttat tcagttactt aggcaacatt tgagatacga ttgcgattat aaaaattgat
     1561 ataccaacca atggctgctt ggaagactga catattagcc tcgatatcat cgaataacta
     1621 aatacagaaa aatacaataa atattatcaa actagggact atactagaaa acatcagtac
     1681 attccttaac tcagggtcta taattagaaa atcgcccctt tgaaaactga ttaaaacagc
     1741 ccccattatc tagtgactag ataatggggg ctgttttttt gttaagtgat ataataaacc
     1801 ataaaagaag tttagaaata cggtcctggt catcaataaa taaacaattt aattaattat
     1861 ttatttaatt atttaatttc agaaaggtat acatgaatca tggtgcaaca aattgtccta
     1921 cccatcaaag actccagcgt cttaaagatg gtacaagata cacttctcga tagtttccgt
     1981 gctggtcgcc gaaattacac cgtttttcaa gttggtaagg ccacgctact acgtgtgagt
     2041 gatgtcatga cattaaaaaa atcagatgtc tataatccag acggctctgt caaaaatgca
     2101 acctttattc atgataaaaa gaccggtaaa gcaaacacgt tatatttaaa gcctgttcag
     2161 caagacttgt tgcaatatca tgattggctg gcccaagaaa atatcaattc tgagtggtta
     2221 tttccctcga cagcccatta tgatcttcat atcactgaga agcagtttca taaggtgatg
     2281 gcgcatgttg gtgatctctt aggaatcaac ttggaagaca aagagaaaaa attgcgagtc
     2341 agaaaaccgt tttgagatat gaacagcatt tgccttcacg ccttagcgat tttaaataat
     2401 agccttgaaa gtgattatgg aaaagccctc tacgctcaat gcaaaattga cgtagaaact
     2461 atttttgatc gattgaaagg tgtatttggt atgtgaagaa cccatgtcag aggcaagaac
     2521 gttgcaccta ctgacattgg aatgatgctt atgagcatga atctaaccaa attatccctt
     2581 gaggctagaa gaaaagcgga agcttttcaa cacaaagcag ccaaaaataa aaaacgcgac
     2641 gaagcaatca catttatgat tattcatcgc gttttttata tttagagcta gttatttgct
     2701 agacccacta tttacagatg aaacgtttat cacctttggt tttgggcatt tagtggaatt
     2761 agcagaacca ggtcattatg acgaaaagtg gcaaaattgg aaacttgaat ctttgccgat
     2821 ttttcctgat cgatacgatt ttgaagttgc aaaagataag ggaaagcagt ttaaaattgt
     2881 tgcagaactt ctcaaaaagg caaatacaat tattgttgca acagatagcg acagagaagg
     2941 tgaaaatatc gcctggtcga ttatccataa tgcaaatgcc ttttcaaaag ataaaacatt
     3001 taaaagacta tggatcaata gcttagaaaa agatgtgatc cgaagcggtt ttcaaaattt
     3061 gcaaccagga atgaattact atccctttta tcaagaagcg caaacacgcc aaattgccga
     3121 ttggttgatc ggcatgaacg caagcccttt gtatacgtta aatttacaac agaagggcgt
     3181 acaaggtaca ttttcactag gacgtgttca aacgcccacc ttatacctta tttttcagcg
     3241 ccaggaagcc atagagaatt ttaaaaaaga accttttttc gaggtggaag ctaagtaaaa
     3301 caagggtcat ttaagggcgt tctaagcccc acacagcgtt ttaaaaccca agaggagctt
     3361 tttagctttt gtttcttcta aacaagctaa aataggcaat caagagggga taattgctga
     3421 tgttcaaacc aaagagaaga aaacgaatag tccgagttcg ttttctttaa gtagtttgca
     3481 atcaaaagtc aatcagcttt ataaagcgac agcgagccaa actttaaaag ctatgcaagg
     3541 actgtatgaa gcaaaattat tgagttatcc aagaacagat acaccattta ttacagagaa
     3601 cgaatttgct tatttaaaag cgaattttgg caaatatagc ggttttttag gacttgatct
     3661 tgaaatgttg ttatcattga taatgatacg ttcaaacaac agcacccaaa ttttgacgaa
     3721 ttagtgaaac tctatgaaaa agacgtagta aaacatgcaa catcctattc taatcagctg
     3781 gtaaagctta attaggttat ataagcttga aaataaaggg tttgtccaat atggacaagc
     3841 ctttttctta tgttttgcca cagatttgcc acacttattg tgtaagagta tcaaggatag
     3901 gtataatttg atactttgtt tttttagtga tgtgcaaata aatttctgtt acacgactac
     3961 ctctagaatg ccctacacgg tcttgaattg cttctagggg tattccttac tctgctaaga
     4021 aactaatatg cgtatgacgg aatatatggg tcgtaatatg tttattaaaa ctaagtttat
     4081 tatcaatatc aattcctgct cgttttggat taccttttcg atttaatttt ccattttcag
     4141 gtgagttgag gaaattgtta aggacactat aatgaagagg tgttccatta tagtcgggtt
     4201 tatgacgact tttggaagta aaaatccatc cattatcttt ataatcagga ttgaatctca
     4261 tatgttggtg atttaactca atttcctctt taagaatttc tatagccctt ttagataatt
     4321 ggacatttcg gtaactttgt aagtttttta cattaccttc atagctattt tctgcagctc
     4381 ctccgtgagt ggactgacta atgatgatat tttgcttttc aaaatattga taggcaccaa
     4441 tattttggag tttcgactct gtaagtcaac agggccatta gttagaatac ctattgggat
     4501 gcggttttta tgtagagcca gcagtagttg atgtatctca ggaatcatcg taatctgatc
     4561 gagttcatat tcatagtcag cttggaagtc tagagcttct gtcctagaaa tactatcgat
     4621 gccaaattct tccatagtct tctgacagcg aaaaattcta agctgctcaa tactgatgag
     4681 ttttttcgtg tatttaggaa aagcaacatc agaccaatat ctgaaacgtt tgtaaatgtt
     4741 atctatttgt tggatatcaa tcgttggaaa acatttctca acgctacgtt taaaggggag
     4801 ttgatggtca taaagagtgt catcaaggtc aaaaacaata tttttataag tcataattct
     4861 agtccgcttt taaaatagta gtgtactggc taaattcctc taagtgttga gggctaatct
     4921 ggttatctgt aatgatggta tcgagttgat ctagattata gaagttaaag aaatcgtatc
     4981 gatcgaattt agtactgtct actaataaga atttttctac agcattgttt agggcaagtt
     5041 gttgaatcac accttccttg tcactatatg tagcaataga attatgggta acagcatttg
     5101 cactaacaaa agctttggca aatctaattg cttttaaatt tgtcgaagcc attgaaccta
     5161 caaaagctcc agttatttct ctatattcac cgccaagaag caataaatca atggtttcgc
     5221 tatcatttag aatcaagaac acagggagac tatttgtaat gacacgaatt ttataacctt
     5281 tacgaccttt caattctaat gccagttgtt caagtgtagt tccaggtcca ataaaaatag
     5341 tatcgccatc tttgattaaa gagcaggctt ttttagcaat gtcaattttt tcttttgtat
     5401 ttagactttt cttctcgata tgtgtctttt caagtggctt tttagaggaa agcaattgtg
     5461 caccaccatg agtacgtgtt aaaagtccat cagcttctag agcatcaagg tctctacggg
     5521 ctgtcatatc ggaaatatct agttcatcta ttatttcttt tatggttatg gtcccatcaa
     5581 tctttaacat atctaaaatt ttttctaatc gtcgtttttt gttcatatga agacccttct
     5641 ttcataaagt aatttttttc caaagataat tctcttttaa ttgtatcata aaagacaata
     5701 ttttcaaggt aaaacaaaca atttcaaaca aaaacaaatg ttagatgatg aaataagaac
     5761 agaggattga cgtatattag cttaggtcag attttgtata agacgaaaat aaagtaggac
     5821 ctcttaatca gtaagttata gaaagtaaaa gacttttgta atacttgaat agatatttca
     5881 cgtccatttt gtgatggatt aaatgaacaa aaatgaacaa taatttaacg gtgttatcta
     5941 ttttttaaaa aaacaaataa aaaaaacaaa aaataaacaa aaaaagtgtt gctttttgtt
     6001 tgaatgcttg atattatata aacaaagaaa tgatgaaaac gttttttcga acattttgca
     6061 aaatgttttc tacttctacg tagcatttct ttctaaaatt taggaggtgg tcaaaatggc
     6121 tattgttgtt ggtgcagatc tcaaaggtac gagattaaaa gatgttgtga aaaattttct
     6181 tgtagaagaa ggttttgagg taattgatgt gactaaggac ggacaagatt ttgttgatgt
     6241 taccttggca gttgcctctg aagtaaataa agatgagcaa aacttaggta ttgtaattga
     6301 tgcttatgga gctggtccat tcatggtagc aactaaaatt aaaggtatgg ttgcagcaga
     6361 agtttcagat gaacgctcag cttatatgac acgtggacat aacaattcac gaatgattac
     6421 ggttggcgct gaaatcgtcg gtgatgagct tgctaaaaac atcgctaaag gtttcgtcaa
     6481 tggtaaatat gatggcggac gtcatcaaat ccgagtagat atgcttaaca aaatgtgtta
     6541 agaaaaggag ttaaggaatg agaatcgcaa ttggatgtga ccacattgtc acagatgaaa
     6601 aaatggcagt atcagaattc ttgaaatcaa aaggacatga ggtccttgat ttcgggacat
     6661 atgaccacgc acgtactcac taccctatct atggtaaaaa agtcggtgaa gcagtagtaa
     6721 gtggtcaagc agacttggga gtatgtatct gtggtacagg tgtcggcatt aacaatgcag
     6781 tcaataaagt tccaggtgtt cgctcagcgt tggttcgtga tatgacatca gctatatatg
     6841 caaaagaaga gttaaatgcg aatgttatcg gtttcggtgg aaagattact ggtggtcttc
     6901 ttatgaatga catcattgaa gctttcattg aagctgagta caaaccaaca gaagaaaata
     6961 aaaaattgat tgcaaaaatc gaacacgttg aaacacataa tgcacatcaa gcagatgaag
     7021 atttctttac agaattcctt gaaagatggg accgtggtga ctaccacgat taaggtataa
     7081 caaatgattc tgacagtcac actaaatcct tcagtagaca tctcttatcc tctagaaaca
     7141 ttgaaaattg atacagtgaa tagggtgaaa gatgttagta agacagctgg tggaaaaggc
     7201 ttaaatgtta ctagagtctt gtatgaatct ggtgataaag ttactgctac aggtttctta
     7261 ggtggtaaaa ttggtgaatt tattgaaagc gaattagaac aatctcctgt tagtccagct
     7321 ttttataaaa tctcaggaaa cactagaaac tgtattgcta tcttacacga gggtaaccaa
     7381 acagaaattt tggaacaagg accaacaatt tctcatgaag aagcagaagg ctttcttgac
     7441 cactatagca atcttattaa acaatcagaa gttgtgacaa tttcaggaag tttaccttca
     7501 ggacttccca acgattatta tgaaaaactt atccaacttg cttcggatga aggagtagca
     7561 gtcgttttgg attgttcagg tgcacctctt gaaacagttc taaaatcgtc ggctaaacct
     7621 actgccatca aacctaataa tgaggaactt tcacagttgc ttggaaaaga agtaacaaaa
     7681 gatattgaag aactcaaaga cgttcttaaa gagtcacttt ttagtggtat cgaatggata
     7741 gtggtctcat taggtcgaaa cggtgccttt gcaaaacatg gtgatgtttt ctataaggta
     7801 gatattcctg atatcccagt tgttaatcca gtcggatcag gggactcaac ggtagctggt
     7861 attgcatcag ctttaaatag taaaaaaagt gacgctgact tattaaaaca tgcgatgaca
     7921 ttgggtatgt taaatgctca agaaacaatg acagggcatg tgaatatgac taattacgaa
     7981 acactaaact cacaaattgg agtaaaagag gtataaaaat ggtacttaca gaacagaaac
     8041 gcaaatcttt ggaaaaaatt tcagataaaa acggtgttat ctcagctttg gcatttgacc
     8101 aacgtggtgc tttgaaacgt ttgatggcac agtatcaaga tacagaacca actgtagctc
     8161 aaatggaaga actaaaggtt ttggttgctg atgaacttac aaaatacgct tcttcaatgt
     8221 tgcttgaccc tgaatatgga cttccagcaa caaaagcatt ggataaagaa gcaggtcttc
     8281 ttcttgccta tgaaaaaaca ggttatgata catcaagtac aaaacgtttg cctgactgct
     8341 tggatgtttg gtctgcaaaa cgtatcaaag agcaaggcgc tgatgccgtt aagttcttgc
     8401 tttactatga tgtagacagc tcagatgaac ttaaccaaca aaaacaagct tatattgaac
     8461 gtgttggttc tgaatgtgtc gctgaagata ttcctttctt cttggaaatc cttgcttatg
     8521 atgaagaaat ctcagatgca ggttctgttg aatatgcaaa agtgaaacct cgcaaagtca
     8581 ttgaagcgat gaaagttttc tctgatccac gctttaacat tgatgttctt aaagtagaag
     8641 taccagttaa cgttaaatat gtcgaaggtt ttgctgatgg tgaagtagtt tacagtaaag
     8701 ctgaagctgc tgacttcttt aaagcacaag aagaagcgac taaccttcca tacatctacc
     8761 taagtgcagg tgtatctgct aaattgttcc aagaaacatt gcaatttgct cacgactcag
     8821 gtgccaagtt taatggtgtg ctttgtggac gtgctacttg ggcaggatct gtagaacctt
     8881 acatcaaaga aggtgaaaaa gcagcacgtg aatggttgcg tactactgga ttcgaaaata
     8941 ttgatgaact taacaaagtt cttgttaaaa cagctagtcc atggactgat aaagtatagt
     9001 aaaaacataa aacggaggat attgttatga acagagaaga gatgactctc ttagggtttg
     9061 aaattgttgc ttatgctgga gatgctcgct ctaaactttt agaagcgctt aaagcggctg
     9121 aaaatggtga tttcgctaag gcagatagtc ttgtagtaga agcaggaagc tgtattgcag
     9181 aggctcacaa ttctcagaca gctatgttgg ctcgagaagc ttctggggag gaacttccat
     9241 acagcgttac catgatgcat ggtcaggacc acttaatgac tactgtctta ttaaaagatg
     9301 tgattcatca cctcatcgaa ctttataaaa gaggagcaaa ataatgcata aactcattga
     9361 acttattgag aaagggaaac cgttctttga gaaaatctct cgaaacatct atcttcgtgc
     9421 tattcgtgat ggatttatcg ctggtatgcc agtcatcctt ttctcatcta tctttatcct
     9481 tattgcctat gtcccaaatg cttggggatt ccactggtca aaagacattg agaccttctt
     9541 gatgactcct tatagctatt cgatgggtat cctagcattc tttgttggtg gtactaccgc
     9601 taaagctttg acggactcaa tgaaccgtga cctacctgcg accaatcaga ttaacttctt
     9661 atctacgatg ctagcgtcta tggtagggtt ccttttgatg gcagctgagc ctgcaaaaga
     9721 aggaggcttc ttgacagcct tcacgggaac aaaaggtctt ttgacagcat ttattgcagc
     9781 ctttgtgaca gttaatgttt acaaggtctg tgtgaaaaat aatgttacta ttcgtatgcc
     9841 tgaagaggtt ccaccaaata tctctcaagt atttaaagac ttgattccat tcactgtagc
     9901 ggttgttctc ctttatggat ttgaactcat tgttaaggga actcttggtg taaccgttgc
     9961 agaatcaatc ggtacccttc ttgctcctct cttctcagct gcagatggtt accttggaat
    10021 tacacttatc tttggtgctt atgccttctt ctggtttgtt ggtatccacg gtccttcaat
    10081 tgtcgaacca gcaatcgctg ctatcactta tgccaatatc gatgccaact tgcatcttat
    10141 ccaagctgga caacatgcag acaaagttat cacttctggt actcaaatgt ttattgttac
    10201 catgggtgga acaggagcta cattgattgt tccattcttg ttcatgtgga tttgtaaatc
    10261 agaacgtaac cgtgccatcg gacgtgcctc agttgttcca acattctttg gggttaatga
    10321 gccaatctta tttggtgcac caatcgtatt gaatccgatt ttctttgtac cgttcatttt
    10381 cgctccgatt gtcaacgttt ggatctttaa attctttgtt gacacattga acatgaactc
    10441 attctctgcc aaccttccat gggttactcc tggtccgttg ggaattgtac tcggtactaa
    10501 cttccaagtt ctatcattta tcttagccgg actcttggta gttgttgata ctatcattta
    10561 ttacccattt gttaaggtat acgatgaaca aattcttgaa gaagaacgtt ctggtaaaac
    10621 aaacgatgct cttaaagaaa aagtagctgc aaacttcaat actgctaaag cagatgccgt
    10681 tcttggaaaa gcaggcgttg ctaaagaaga tgtagcagca aacaataata tcacaaaaga
    10741 aactaatgtc cttgttcttt gtgcaggtgg aggtacaagt ggcttgcttg ccaatgcctt
    10801 gaacaaagca gcagcagaat acaatgttcc agtcaaagcg gcagcgggta gttacggtgc
    10861 tcaccgtgaa atgttgccag agtttgactt ggttatcttg gctccacagg tcgcttcaaa
    10921 cttcgatgat atgaaggctg aaacggataa attgggcatt aaacttgcaa aaactgaagg
    10981 agctcaatat atcaaattga cacgtgatgg acaaggtgct cttgcatttg ttcaacaaca
    11041 gtttgattaa taaatttgta aaagttattt gagacgagat tattctttca attccttata
    11101 atattgttaa ccatatcatt gcatttcatt tccttggttt ccggaactca gaatatcgtc
    11161 tcaaatgctt tttttgaaag gatttatagt tatgactaaa acacttccta aagattttat
    11221 ttttggtggt gctacagctg cttatcaagc agaaggtgcg actcacacgg atggtaaagg
    11281 cccagttgca tgggataagt atcttgaaga caattattgg tacacagcag agcctgcaag
    11341 tgatttttac cataaatatc ctgttgattt agaattagct gaaaaatatg gcgttaatgg
    11401 tattcgtatt tcaattgcct ggtctcgtat tttcccaact ggctatggcg aagtaaatga
    11461 aaaaggtgtt gaattctacc ataatctttt tgccgagtgc cacaaacgtc atgttgagcc
    11521 attcgtgact ttgcaccact ttgacacacc agaagttctt cactcagatg gagatttctt
    11581 aaaccgcgaa aatatagaac actttgtaga ttatgccgct ttctgttttg aagagttccc
    11641 agaagtaaac tactggacaa ctttcaatga aattggccca attggagatg gtcaatactt
    11701 ggttggtaaa ttccctccag gcattcaata tgatcttgcc aaagtcttcc aatcacacca
    11761 caatatgatg gtttctcatg cacgtgctgt gaaattgtat aaagataaag gttataaagg
    11821 cgaaattggt gtagtacatg cgttgccaac caaataccct tatgatccag aaaatccagc
    11881 agatgttcgt gccgctgagt tagaagatat catccataac aaatttatct tggatgcaac
    11941 ctaccttgga cactattcag ataaaacgat ggaaggggtc aaccacatcc tagctgaaaa
    12001 tggtggagaa cttgatcttc gtgatgaaga cttccaagca cttgaagcag ctaaagattt
    12061 gaacgatttc cttggtatca actactacat gagtgattgg atgcaagcct ttgatggtga
    12121 gactgaaatc attcacaatg gtaagggtga aaaaggaagt tctaagtacc agattaaagg
    12181 tgttggtcgc cgaatcgcac cagattatgt gcctagaacg gattgggact ggattattta
    12241 tcctgaaggc ttgtatgacc aaatcatgcg agtgaaaaat gattatccaa actacaagaa
    12301 gatttacatc actgaaaacg gtcttggata caaagatgag tttgtagata ataccgttta
    12361 tgatgatggt cgtattgatt acgtgaagaa acacttggaa gttttatcag acgcaattgc
    12421 ggatggtgca aatgttaaag gttacttcat ctggtcactt atggatgtct tctcatggtc
    12481 aaacggatac gaaaaacgtt atggactatt ctatgtagac tttgacacgc aagaacgcta
    12541 tcctaagaaa tcagctcatt ggtataagaa agtagcagaa actcaagtga tagagtaata
    12601 ttacttgaaa atgtaaaaaa ttgttatccg atactttaga aaaagtacaa cacttattta
    12661 gaccaggctt tgaaaaggta tatattgtta gtttcgaaga ttgtcctatg attcctgagc
    12721 ttgaagctac cccgttgcta aaatgtggta aatggtatgt ttcaactgga aaagagtgga
    12781 tttgccattc tgatttagag ttatcagcat ttgaatggga atttttacaa tcactcgatg
    12841 tagaaatacg tgagactatt cacttcgagg taaattattt gcctttccaa taataaaaag
    12901 aaaggaatta gttgtgacta tcgaattaaa aaacgagtat cttactgttc agtttaaaac
    12961 acttggtggt caactgactt cgattaaaga caaggatggg atagagtatc tctggcaagc
    13021 tgatccaaat tattggaatg gacaggcgcc tattctattt cctatctgtg gtagtttacg
    13081 aaatgattgg gctatttata gacctcaaga aagacccttt tttacaggtc ttattcgtag
    13141 acatggtttt gttcgaaaag aagaatttat tctggaagaa gtcaatgata atagcgtgac
    13201 ctttagtatt aaaccaaatg ctgagatgct tgataattac ctatatcagt ttgaactgag
    13261 agttgtttat accttgaatg gtaaatcaat tagaaccgaa tttcaggtaa cgaacttgga
    13321 aatggaaaaa acaatgcctt actttattgg agcccatcca gcattcaata gtcctttagt
    13381 agaaggtgaa aactatgagg attattatat tgaatttagt gaggttgagt cttgctcgat
    13441 accaaagagt tttcctgaga caggattatt agatttgcaa gatcggacac cttttttgga
    13501 aaatcaaaaa attctcgatt tggactactc acttttttcc catgatgcca ttacacttga
    13561 ccagctcaag tctcgtagcg taacgcttcg gtcaaggaag tctggtaaag gtcttcgagt
    13621 agattttgat gattttccga acttgattct gtggtctaca actaataaaa gtccattcat
    13681 tgctttggaa ccttggagtg gtctctccac ttcacttgaa gaaggtaatt tcttagaaga
    13741 taaaagacag gtaacgaaaa tttcacctca agaaacttct agaaaaatct atgatattac
    13801 tatccttaat taaaatttgt tattatttga gtcttcaata agatttgaat actcttacct
    13861 tgtgatgata gattggtctt ctccgtgcca gtcttacatc gcaaggtttt taagtttttt
    13921 gtgggtctat gtcaactgta gtgggtgacg aaaagctaat atctaaagac agtctggtag
    13981 taaaaaataa gattatttac tacttcttga tttaaaataa tagtggagcc attaatttgt
    14041 ggcaaattta atttaatatt ttgcctcaaa ttagccacac tttttataga actaagagga
    14101 aaaatgaaat tttcttccta ttaatatagt tgtttgaaat cttaaatata gcgt
//
LOCUS       DASHRQ010000069        14118 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.073, whole genome shotgun sequence.
ACCESSION   DASHRQ010000069 DASHRQ010000000
VERSION     DASHRQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.073"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..556)
                     /locus_tag="U2M88_002271"
     CDS             complement(<1..556)
                     /locus_tag="U2M88_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEM8374623.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
     gene            complement(572..1051)
                     /locus_tag="U2M88_002272"
     CDS             complement(572..1051)
                     /locus_tag="U2M88_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEM8374624.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1057..2081)
                     /locus_tag="U2M88_002273"
                     /pseudo
     CDS             complement(1057..2081)
                     /locus_tag="U2M88_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2098..3204)
                     /locus_tag="U2M88_002274"
     CDS             complement(2098..3204)
                     /locus_tag="U2M88_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEM8374625.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="U2M88_002275"
                     /gene_synonym="adhC"
     CDS             complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="U2M88_002275"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEM8374626.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="U2M88_002276"
     CDS             complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="U2M88_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEM8374627.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="U2M88_002277"
     CDS             complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="U2M88_002277"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEM8374628.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8175..8873
                     /locus_tag="U2M88_002278"
     CDS             8175..8873
                     /locus_tag="U2M88_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEM8374629.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9041..11680)
                     /locus_tag="U2M88_002279"
     CDS             complement(9041..11680)
                     /locus_tag="U2M88_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEM8374630.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12299..13330)
                     /gene="queA"
                     /locus_tag="U2M88_002280"
     CDS             complement(12299..13330)
                     /gene="queA"
                     /locus_tag="U2M88_002280"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEM8374631.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13523..>14118
                     /locus_tag="U2M88_002281"
     CDS             13523..>14118
                     /locus_tag="U2M88_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8374632.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         3959 a         3096 c         2543 g         4520 t
ORIGIN      
        1 aagcgcctac gagataacct cccataaaca aagcagagat cgaagcacca ccagcaacaa
       61 gtgaatacat gataaaagca gtacttggag gaatgaccat acctgtcgga gctgacgcga
      121 tattgaccgc tgtcgcaaac ttccggtcat atccttcttc cacttgctga gggatcaaca
      181 ctccaccaat cgcggtagaa gcggcaatgg ccgagctaga gatggaacca aataaagcat
      241 tccctaacac attcgtatga gccaaagccc ccggcacgcg cccgacgaac aacatcgcaa
      301 aatccactag tttcttagct atccctccat tattcatgat aatgcccgag agaacgaaaa
      361 acggaatagc gatcagcgaa aaactattga tacttcccac cattttttga gctgaactaa
      421 aagcagaaac atcaaacggt ataactaaca ataaagtcat catcgatgag aatgcaatac
      481 ttatagcaat cggcatccct aatacaagca acacaacaaa tacaaaaaat aacactaatc
      541 cggcttctat tgccatttta ttcctccatt tttatatttt ttcttgaatt gctggcttgt
      601 tgcttaatgt atcgattaga ttgatcaggc tatagataat gatgagtacc cccgatacag
      661 gtactgacag ataaacatat cccataggaa tacttaaaga aggcgagatc tgccccattg
      721 tcaatgtgac agcattccat ccgccataca ccatgatcat cgcagcaaat accagaaata
      781 aactttgaac aacaacttct aaaattttac tttttttgcc agttaatttt tcacttaaaa
      841 ccgttacggc taaacgtgct ttttgtccca caacatacgc ggaagacaac atggcaaacc
      901 agacaagacc gaatcgaaca agctcttccg tgaccgtatt tggatttcct aaaaccgttc
      961 tggaaaatac ttgccataaa acaacagcga ccatcccaac aagcaaaaca gctcccagga
     1021 tctctaatac cttgtttaaa atcattttca ttacagctac gctccttgct cttggatctt
     1081 ttgataaagg tctttgtagg taggatcgtt tttgaatata tcatgaagtg gggcaactgc
     1141 ctctcgaaac ggtgtattat ctacatcatt gaattggact ccatgttctt cttgtgctaa
     1201 agctttttct tcttcaattg cttttgcaaa agcttcaatt tcaaaaaccg ttgattcttt
     1261 cgctgcttct tcaatggctt tttcctgttc atcagttagc tgttctttga tggcatcatt
     1321 catgatgatg atatctggca cacgtgtatg ctcatcatag gaatagtatt tagctacccc
     1381 gccatgtcca gctgtataca agacaaattc attgttttct gatccgttga tcaaattgga
     1441 ttgaagagac gtgtacactt catcacttcc cataggtacg ggcgaaccgc ctaaaagctc
     1501 taccattttg atagcggttt cgctttgcat cacacggatc ttctttccct tcaaatcttc
     1561 cggcgtatgg atcggaccgt ctaccatata aaagctgcgc tgaccagaat cataataggt
     1621 caatccggta aatcctaaat ttttggtcga ttggtagatt ggctccatta tttcttcatt
     1681 atccatcacc ttaaagaaat gttcttcatc attgaataga taaggaacgg aaaaaatcga
     1741 ataatcttta gagaaacttt ccaacgctga ggcacttact tttgtgaaat ctactgctcc
     1801 tgactgtgtc aattcgatca gttcacgttc tccacccagt tgtccatctg ggaaatactg
     1861 gatctgtacg gaaccatctg ttttttcttt tactaaacgc ccaaattcat tcattgcatc
     1921 aattactggc tgttgcttgc ataagcaaag cgcaaggtaa ctatctcatt tacgtctgca
     1981 tctgcgacag ttgccacact agaattattt ccgcagccgc ttagacctag cagacctagg
     2041 ctaagcgaac ttgctgcaaa cagcagtgat gtttttttca tatgggtttc ctttctttta
     2101 acaataagag acgagcaatt ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat
     2161 tgctgtgatt ccgatattcc gataaaaatc taaactgtcc cccattcctg tcccgattga
     2221 aacgttttga acttctcctt tttcatcgat ttgttcaagc aatccttgga tagcacgata
     2281 agcgacttct ttatattctt gtggtaaata acgtttatgg accgctttca aaataccgta
     2341 agcaaatcct gcagttgcag aagattctac gtaagaagaa ggatcatcca acaacgtatg
     2401 ccataaaccg ctttcagact gataagtttt taatgcacgc acttgtgctt caagtgtttc
     2461 gatcaataat ttgcgcaatg catcttcttt cgataattct aagatttcaa tgatctctgg
     2521 aatagcaatc gtcaaccagc agtttccacg agcccaaaat gcttcggcat agttatgatt
     2581 tccttcaaac gtccagccgt gataccacaa tcctgttcgt ttatccatca agtatttgat
     2641 atggatcata aactggtaac gagcttcttc taaatattct gggcgattca acagtttccc
     2701 gatttttgcc aaaggaagca ctgtcatcat caatgtatcg tcccacaact gattcttatt
     2761 ttccggtcca tatgtggcat gctgtaaccc gtcttcattt gtccgaggca tgtcattcat
     2821 gacccattct gcccattgtt ctaaataagg caaatagcgt gaatctttcg tgtcttcata
     2881 caagtaagcc atagtcaata gcggtgccat tgtattgacg tttttgggcg gagcgccttc
     2941 ttccatacga tcctcaaacc attcattgac cacttgataa gcgcgaggat tttttgtcat
     3001 ctggtaattt ttataaatac catatagacc gacaccttgt ggccaattcc acactgtcca
     3061 gcttttgtca tctactttca gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt
     3121 gatcgttgtc aggttttcaa cgagcaggtc aattttttct ttcactaatt ctttaggtac
     3181 aaaaatctct tctttaataa tcatctttac gccacctttt taaacgcttt cattttatat
     3241 ccttaatata gcagtaatac cgtccactat aaatatcgct ttttgaacaa aaactgtcta
     3301 aaattgacct aaatgttaaa taacaaaagt aactttcagt tacttggaaa ttaaaaaaag
     3361 aacttggaga aacaacgttt tccaagttct ttttcatcta ttttcttatc ttttttctac
     3421 gctttctttt gcttatccgt tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa
     3481 tggttctttc ggattagctg tcgtacattg gtcttcgtaa gctaattcag ccatacggtc
     3541 tacggttgta tctagtgttt cttgtgtgac tccttgagct tttagactca tgttgatgcc
     3601 gacagatttt cctaattcat aaacagccgt tgctaaagct tcgactaatt cctctgttgt
     3661 ttcgcctttc agacctaaga attttgcgat atctgcgtaa tctgtatctg cacggaaata
     3721 gtcatattta gggaacatcg catgtttttg tggatctttt gcattatagc ggataatatg
     3781 cggtaaaaga atcgcatttg tccgtccatg agggatcccg tattccccgc cgattttatg
     3841 tgcgactgag tgacaaatac ctaggaatgc gttagcaaac gccataccag ccattgtaga
     3901 agcgttgtgc attttttcac gtgattccat atcaggtgtt tttactgatt tttctaaata
     3961 gtcaaagact aacttgattg cttgtaggct caatccacgt gtataatctg aagccatgac
     4021 tgaaacatag gattcgattg catgtgttaa cacatccatc ccggtatctg ctgtgacaga
     4081 tgctggaact gacatgacga attgaggatc gacgattgcg acatctggcg tcaatgcata
     4141 atctgctaac ggatatttca catgtgtttc actgtcagtg atgaccgcaa atggcgttac
     4201 ttcagaacca gtccctgatg ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc
     4261 gatcttatat gtccgtttac ggatatccaa gaatttttgt tttgcaccaa agaaagatgt
     4321 gtctgggtgt tcatagaaca tccacattcc cttagctgcg tccattgcag aaccgcctcc
     4381 aagagcgatt actgtatctg gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt
     4441 atttgtcgat gggttcggtt caacatctga gaagacttcg atcttcacat catttttacg
     4501 tcgtgccaat acttcacgaa ctgtatcgca gtaaccgaat tggaccatac cagggtcaca
     4561 aacgatcatg acacgttcaa cgttttccat tttctctaag tacaatagag agttcttttc
     4621 aaagaagatt tttggcggta atttgaacca ttgcatgtta tttctccgtt tcgctacagt
     4681 tttgacattg attaagttta cagcagagac gttacgagaa acagagttct taccataaga
     4741 accgcatccc aaagtcaatg acggaatcat ttcgttatag atatcaccga tgccgccttc
     4801 tgcagatggt gtattcacta aaatacgaca agctttcatt cgaagaccga atttgacatg
     4861 caactcttcg ttttctgaat ggataactgc tgtatgtcct aatcctccaa gattcaacat
     4921 accttcacac aaatcaaacg catggtccgt gttgttagct ttgatcatgg caagaactgg
     4981 agaaagtttt tctcttgata gcggataatc tgggcctacg ccttccaatt cagcaatcag
     5041 cattttcgtt ccttttggta cactgattcc tgccaatttt gcgatttcca tcgcagaatg
     5101 tccaacgatt gatggattga ccgcatattt tccttcgttc atcactgcat cttctaattt
     5161 ttgcaattca tctggtttta cgatataaac ttggtgtgct tggaattctg cttttactgc
     5221 tgcatagatt tctttatcca cgatcacagc ttgttcagaa gcacaaatca ttccattatc
     5281 aaatgttttc gaaacgatca aatcgttcac tgcacgttta atttttgctg ttttttcaat
     5341 ataagctggt acgttacctg gacctacacc taaagctggt tttcctgtag agtaagcaga
     5401 cttgaccatt ccagctccac ctgtcgctaa gacgatcgcg atacctggat ggttcattag
     5461 catagaagtc gcttcgatcg aaggatgttc gatccattgg atacaatttt caggagctcc
     5521 ggcagcgata gcagcatcac gtaccactcg tgctgcttct gcagaagatt tttgggcact
     5581 tggatggaaa gcaaaaacga ttggatttct tgttttcaac gcgatcattg atttgaaaat
     5641 cgttgtggaa gtcggattcg ttgttggagt tactccgcaa accacaccga ctggttcagc
     5701 aatttcaatc aacccttttt ggacatcttc attgatcaca ccaactgttt tatcatgttt
     5761 gatattatgc caaatatatt cggacgcata catatttttg attgctttat cttcgtaaac
     5821 accgcggcct gtttcctcta cagccatctt cgctaatggc atatgttgat ccaaagctgc
     5881 cattgccatt tcatgcacga tatggtctac tttttcttga tcaaagcttt ccatttcttt
     5941 caatgctgca tttgcttttt ctgccaaatc atcaatcatt gcttgtacgt caattgtctt
     6001 cgttttttct ttttctatta ctttagccat cattatcctc ctaatgggtc atggttattt
     6061 gttaaataat tcacatgtat atcatacaat atttattttt ctatgtaaac acttttttgt
     6121 gatttttttc acagttattt ttaaatataa aattgcttta tttacaggtg tttgtataga
     6181 aaaatatata aagttattta ttattattta tatgacacac ccgattattc aatattgagc
     6241 aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt
     6301 ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct
     6361 cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg
     6421 tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg
     6481 ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat
     6541 tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt
     6601 tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa
     6661 aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt
     6721 tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta
     6781 gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga
     6841 acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt
     6901 gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa
     6961 tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac
     7021 atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt
     7081 tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca
     7141 agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac
     7201 cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata
     7261 cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga
     7321 aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt
     7381 ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg
     7441 cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg
     7501 gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt
     7561 tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt
     7621 cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa
     7681 tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac
     7741 cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca
     7801 actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg
     7861 tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt
     7921 tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa
     7981 aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg
     8041 cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa
     8101 agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa
     8161 aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa
     8221 aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta
     8281 ctattcttct tattatgatt cctattatct tgtttgccac caccatatat gatggatcag
     8341 cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta
     8401 tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt
     8461 tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt
     8521 tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg
     8581 gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta
     8641 tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg
     8701 caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat ctttcattta
     8761 tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat
     8821 acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag
     8881 tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa
     8941 acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt
     9001 atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt
     9061 ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc
     9121 ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg
     9181 gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt
     9241 tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca
     9301 gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt
     9361 ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga
     9421 tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt
     9481 catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag
     9541 caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg
     9601 gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc
     9661 ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag
     9721 cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca
     9781 catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat
     9841 cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga
     9901 tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat
     9961 ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat
    10021 tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca
    10081 tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg
    10141 gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag
    10201 aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc
    10261 gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg
    10321 gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa
    10381 ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt
    10441 cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg
    10501 ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata
    10561 gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta
    10621 cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg
    10681 ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga
    10741 tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa
    10801 cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa
    10861 ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga
    10921 tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag
    10981 tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga
    11041 cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt
    11101 cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt
    11161 ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg
    11221 cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact
    11281 caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca
    11341 aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca
    11401 caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg
    11461 tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc
    11521 gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct
    11581 tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta
    11641 ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc
    11701 ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa
    11761 agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg
    11821 ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag
    11881 aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga
    11941 catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag
    12001 tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt
    12061 gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta
    12121 acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt
    12181 ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt
    12241 tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt
    12301 atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct
    12361 ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg
    12421 attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg
    12481 taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa
    12541 tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac
    12601 gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat
    12661 ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg
    12721 tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat
    12781 gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc
    12841 gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt
    12901 caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca
    12961 attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt
    13021 tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa
    13081 gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa
    13141 ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga
    13201 aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc
    13261 ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg
    13321 ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg
    13381 ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac
    13441 taccctattt gtattttttc attgttttag gcatactagt aatagatagt tcttcattga
    13501 aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg
    13561 tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt
    13621 tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga
    13681 tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga
    13741 attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat
    13801 ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa
    13861 aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc
    13921 acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa
    13981 tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc
    14041 cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac
    14101 ccatgacatg gatgaagc
//
LOCUS       DASHRQ010000070        14066 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.074, whole genome shotgun sequence.
ACCESSION   DASHRQ010000070 DASHRQ010000000
VERSION     DASHRQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.074"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(451..1761)
                     /locus_tag="U2M88_002282"
                     /pseudo
     CDS             complement(451..1761)
                     /locus_tag="U2M88_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
     gene            complement(1773..3191)
                     /locus_tag="U2M88_002283"
     CDS             complement(1773..3191)
                     /locus_tag="U2M88_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM8374633.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="U2M88_002284"
     CDS             complement(3431..4999)
                     /locus_tag="U2M88_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEM8374634.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="U2M88_002285"
     CDS             complement(5003..5413)
                     /locus_tag="U2M88_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEM8374635.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="U2M88_002286"
     CDS             complement(5410..6333)
                     /locus_tag="U2M88_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEM8374636.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="U2M88_002287"
     CDS             complement(6330..7208)
                     /locus_tag="U2M88_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEM8374637.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8531)
                     /locus_tag="U2M88_002288"
                     /pseudo
     CDS             complement(7195..8531)
                     /locus_tag="U2M88_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
     gene            complement(8534..9637)
                     /locus_tag="U2M88_002289"
     CDS             complement(8534..9637)
                     /locus_tag="U2M88_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEM8374638.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9711..11219)
                     /locus_tag="U2M88_002290"
     CDS             complement(9711..11219)
                     /locus_tag="U2M88_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEM8374639.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11185..11877)
                     /locus_tag="U2M88_002291"
     CDS             complement(11185..11877)
                     /locus_tag="U2M88_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEM8374640.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11899..12873)
                     /locus_tag="U2M88_002292"
     CDS             complement(11899..12873)
                     /locus_tag="U2M88_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8374641.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13173..14045
                     /locus_tag="U2M88_002293"
     CDS             13173..14045
                     /locus_tag="U2M88_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM8374642.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
BASE COUNT         4145 a         2977 c         2310 g         4634 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gtttcaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtatcctc gatcagtttc ttggaagata caatttgaaa tggtaatatt acgaatacta
     7801 ccactcattt cacttcctaa tacgacaccg ccatgtccat ggaccatcgt gcaatttgtg
     7861 atcgtgatat tttcgcaagc gatcctttcg tacgtatctt cagtccctgc cttgattgca
     7921 atacagtcat ctcccacgtc aatatggcaa ttactaatac gcacattttt gcatgattct
     7981 ggatcaattc cgtctgtatt tggtgaatcc gctgggttca agatcgtcaa gttatcaaac
     8041 gttgcattac tgcaaagaat cgggttgatc gtccaacttg gtgactggat caatttaata
     8101 tctttcacag taattcgatg acagttatgg aaactcatca atttaggacg aggataagcg
     8161 agattatctg gttcatttct aaaagtatgc caccattttt ttccatttcc atctaatgta
     8221 ccgaaaccag tgacagagat gttctctaca ttttgagcat agatacagga agcataaact
     8281 tttcgatgga ctccttccca acgtgaatga acaacaggat aatcttttgg atcgtcagag
     8341 aatttcagaa ccgctcctgc gcttaaatga agttcgacat tgctttttaa aaataaagct
     8401 cctgttagaa attcacctgc tggaacaact acggtcccgc ctccgtcact tgctgcagca
     8461 tcaatggctt gttgtattgc ttcagtattc aattcatcaa ttgatgcgcc aaatgtcaaa
     8521 atatcataca tatttataat gcctccactt caatcaatgc ttgcaagaat gcaccaacgc
     8581 ctttttgatc attacaaata attggttcac tgatgtagta agcatatgaa ccatctcgct
     8641 gatctgctcc gcctaaccct gctacttgac agtttttatt caagttcagc cagccttctt
     8701 ttgtttccag aacaaattca tcaagtaatc cttgatgtga tttcttcact tgtggaagcc
     8761 attcttcttt gttcagcagt cccaaacgta ttcctttagc cattgcgtaa acaaacatac
     8821 tactgccgga agcttccaga taatttcctt tccgttttgt ttctgttgtt acttgatacc
     8881 atgtcccgct gttttcttct tgatatggaa gtaatgcagc cagtgtcttt tctagaattt
     8941 ctttcagcaa agaacaatct gtttcttcat gaagtaattc ataggtatct gctgcagcca
     9001 tcaaatacca gccaatcgat cggctccaaa aatgatggga tagacctgtt tttggatcag
     9061 cccatggttg gaccttcttt tcatcccatg catgaatcag caaatttgtt tttggatcga
     9121 atagatgttt ataactgatt tcaaactgtc tcaaaacatc ttggtaatct ttttcttcac
     9181 taaaattctg gataaattca gcataaaaag gagaacccat atataagcca tccaaccaaa
     9241 tttgataagg ataaatttct ttatgccaaa aagctccttc ggatgtgcgt ggatggtttg
     9301 ccaactgctg ggaaaggagt tctgctgctt ttttatattt ttcttcgcct gtttctttgt
     9361 acaataaaaa gaaaagcttt cctgtattta catggtcgat attgtattct tcaacgctgt
     9421 agccacggat cgttccatct tcttgcacga aatagtcgat attgtctttt atgaaatcaa
     9481 aataaatttt ttcttgggtt cgttcccaaa gtaattggaa ccctttaagt acgacgccat
     9541 agtcataaga ccattttcct ttataccact tttcttcata taagcgaggt gttctttcca
     9601 tgatactttt tgctaatttc tttgaccagt tttccattat ttgctctcct ttaaaaatta
     9661 aaataggatt tgggacaaat accaagaagt atttgtccca atcgtctata ttactgtgcc
     9721 gctgctttgt atgcttcctc gtactcttgt gtgattcgat cgcctccttg tgctttccag
     9781 tcttctacag cttttttgaa tccatcttcg tcaagctgtc ccatgatgta ttgcaccgta
     9841 gcatcaatga cgattttttc aatttctgat cctacttcat tgttcgtcgg tgaatccaat
     9901 ggcacagaag gatccattac agcaaattct tcattttctt tgatcaattg attagagaat
     9961 tctttttcag ggtctgtatc tttgaaagag aacgtcactt cactaggacg agagcttgat
    10021 aacggttgca cctcttgttg ccaaagatcc atatcgataa attcgataga accatcatca
    10081 ttcaattcat aatgcgttcc ttcgataccg ccagtcatca atttatacac atcttcttct
    10141 aaaagatcat caacaaattg aagtaatttc ttcaattgtg cttcgtcttt tacttgagat
    10201 ttgggaaatg ctagcaagcc gccaatacca tttccttctg accaaacatg ataaccgccg
    10261 ccttcaccat ctgagatctt gtttacaggc actaattcca tatcatcttg aataccttga
    10321 cccatcgtct tcagattctt gatatccacc attccagtgt agattccagt tcttccttgt
    10381 gcaaattgtt cttgctgatc agtcttttgg gtaactgcaa agtcttgtgc caaatagcca
    10441 ttctcaaata gatctcttga aaaattcaaa gctttcatat actcttctgt atcaaattct
    10501 ggagtgaatt tgccattatc gtctacagcc cagccatttg gtgctccaaa ataagaagtg
    10561 aaaagtttaa agctggtata acgaatatca ctaggcgcac gatcgccaaa accaactgtg
    10621 tcgtccacac catttccatc aggatcgttt tctgtaaatt gtttagctac ttcataaagc
    10681 tcatccatgg tagttggaac atctagtcct agattatcca accagtcttt tcgaatgact
    10741 aagccagcac gagcgtagtc tttttgtatc ggtacgccgt acaaggcacc gtcgatactc
    10801 gcagcttcga ttctatcttc agaaatttga cttaaatttt tatagtcttt taaatattct
    10861 gatacatccc agaatacgcc tgattttaac gcattgcgga cagaagagtt tttcaacatc
    10921 gcaatttgaa gtgtaacgat atctcctagt tcatcagaag ccaatgcagt tgttaatctt
    10981 tcttcttttg aagcatcagg aatccagttg aatgtcagtt ccgtatcggt tttttcttcg
    11041 atcaagttca caatcgtgtc tgtaggcggt gaagcatgat gcaaaatatt taaccaagtg
    11101 attttagctt ttccatcttc tgtctctgaa gaactgttac ttgaactgct gccgcatgcg
    11161 gcaagtgcta gaattcctgc aaaactaaga cctaatttta cgagcgttgc ttttttcata
    11221 aattttctcc ttcactgttt taataatatt tttgttttct tctgtttttt ctgtatatga
    11281 gttcttccat tccaaatggc aaaatataga atttgctgtc cacgtgactg cggaaaagaa
    11341 gagactgcca ccgataaagg ttaaaacacc tggcattgtt tggatgatca tcagataaat
    11401 ccctaccata cttaatatca acactaatga gtaatgaaga tgtgagaatc cgaataaaaa
    11461 tgccattttc attttgaaaa aatatccttt ccaatcataa tgagccatga ttgggaaaat
    11521 atagagtgct gagagcacat atatgaacgc agcaatatac aaagcgatcc taagatacca
    11581 attgtgtatg aaagaaagat ccacaagtaa aacaatcccg atggcgccat atatccagct
    11641 catcagcaag gtttcacgat agttttcttt aaaatatgtg aagtacgttt tcgtcaaagg
    11701 aaacgtttct tttgagcgca gccattttct aatcactgca accatcgcat acgatgcagg
    11761 accgaaagta aaaactccca aacctgcgat gactaaaccg ccccacagaa aattcaaata
    11821 aaccaactgt aaaatacgat ttagccttcc gtttatttta tctaatgact gcagcatatc
    11881 tttccctctc tttctatctt agtaaacgcc gtcttctcct agctttttgg caatcttatt
    11941 agctaaaatt accataaaca atcctacgac ccctttaaac aatcccattg ctgtactgaa
    12001 acttaactgt ccattcttca atccagctgt ataaatatag gtgtcgaaaa tttctgcaac
    12061 gcttcgattc aatgaattca ataacaagta catatgctcg aaccctaagt ctaatgtctg
    12121 accgattttt agaatcagca aggtaatgat tactggacga attgccggca atgtaacatg
    12181 ccatgttttg cgtaagcgtc ctgctccatc catttctgca gcttcataca actgtgtatc
    12241 gactgcagtt atcgctgaaa ggtagatgat cgtagaccag cccaactctt tccagatcac
    12301 ttgtccgata tatactgtgc gtgtccattc aggggatgtt agaaaactga ttttttcaaa
    12361 tcccattgtt gcaaggaatt cattcaatac ccctccgtca acattcaaaa atacataagt
    12421 aatcgaaaca atgattaccc aagacatgaa gtgagggatg taaatgatcg tttgaatccc
    12481 gtttttcaat cgtttgttcg ttacttcatt taatagtaaa gacaaaatga ttggcatcgg
    12541 aaaaaacacg aagatattta atccgaacaa gatcaacgta tttcttaata atgtaaagaa
    12601 tgtcggttct gtaaacaaac gaatgaaatg tttgaaaccg acccaaggac ttcccattat
    12661 tcctaaaaat ggttgataat cttgaaaagc aataacgagt ccccccatag gaagatacct
    12721 aaaaatcaag aaataaagga ctcccggcag aatcatcaca tataaaaatt tattcgtttt
    12781 gattcctgca attatttttt ttcgtcttct gagcttttct tcttttttcg atagcgtcag
    12841 ttgaaatggt tcttgtttct ttacactttc cataagcccc tccttagttt tgtgaatgtt
    12901 tgctgcttat gacttcatga tagataacta tctgaattac gtatataatc atttttaccg
    12961 taagtaaaaa cgcttacata gcaatgattt ttgtgctata caaaacgata aagtgtattt
    13021 tttcacaaat aaactacgca tagcaaacct tctatttttt tctatctgac acttagcata
    13081 ggaaatgatt atatacgtaa tctttcaccg atgataaatt gaacatatag aaacagaaac
    13141 atttaattta ttttgaggag gagcctatct atatgaaaaa gaaagtctct ataggagaaa
    13201 gaagttttac acttttcaac aatacgtttc taattttgct agctttgatc tgtatcgttc
    13261 cttttttaaa tatcatcgct acatcttttg cttctacaca agaagtcgtc gcgaaaaagt
    13321 ttatcctttt tcctactacc ttttctttag acgcgtatcg ctacattctt tctacgccga
    13381 ccatttttag agcacttgct gtttcgattg gtgttacagg tgtcggaacg atcgtcagta
    13441 tgtgtgcaac ttcactgatg gcctacggtc tttccagaaa gtatttgttt ggtcgaggtt
    13501 tcgtcaactt tcttgttgta ttttctatgt tgttcagcgg ggggatgatt ccaacgttct
    13561 tagttgttcg ttctctaggg ctggtcaatt cttactgggc catgatcctg cctgtggctg
    13621 ttaatgcaat gaatatgatc attatgagaa actttttcca agccttgccg gatagtttgg
    13681 aagaatcagc aaaaatggat ggttgcacag attttggtgt atttttcaaa attatgctgc
    13741 cattagcgtt accgtctatt gctactatct cgctttttta tgctgttact tactggaaca
    13801 cttatatgac tgctattttg tatatcaacg attcatctaa atggccgata cagattcttt
    13861 tacgccaaat cgtcattgta tcaagtggga tgcaagcaga aagtagtgcc gttgatgtga
    13921 ttccaccagc acaaacagtt aaaatggctg ttatcgttat tgcaacagtg cctatgctga
    13981 ttgtttatcc ttttgttcag aaatattttg tcaaaggagc actcgtaggt tctgtcaaag
    14041 ggtgattatg tactgaacgc aaaatg
//
LOCUS       DASHRQ010000071        13945 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.075, whole genome shotgun sequence.
ACCESSION   DASHRQ010000071 DASHRQ010000000
VERSION     DASHRQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.075"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            137..310
                     /locus_tag="U2M88_002294"
     CDS             137..310
                     /locus_tag="U2M88_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374643.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            950..1423
                     /locus_tag="U2M88_002295"
     CDS             950..1423
                     /locus_tag="U2M88_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEM8374644.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1432..1659
                     /locus_tag="U2M88_002296"
     CDS             1432..1659
                     /locus_tag="U2M88_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8374645.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2295..2666
                     /locus_tag="U2M88_002297"
     CDS             2295..2666
                     /locus_tag="U2M88_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374646.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2922..3164)
                     /locus_tag="U2M88_002298"
     CDS             complement(2922..3164)
                     /locus_tag="U2M88_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEM8374647.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3196..4098)
                     /locus_tag="U2M88_002299"
     CDS             complement(3196..4098)
                     /locus_tag="U2M88_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEM8374648.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4111..4299)
                     /locus_tag="U2M88_002300"
     CDS             complement(4111..4299)
                     /locus_tag="U2M88_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEM8374649.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="U2M88_002301"
     CDS             complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="U2M88_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEM8374650.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4914..5807)
                     /locus_tag="U2M88_002302"
     CDS             complement(4914..5807)
                     /locus_tag="U2M88_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM8374651.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5885..6808)
                     /locus_tag="U2M88_002303"
     CDS             complement(5885..6808)
                     /locus_tag="U2M88_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEM8374652.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6857..7207)
                     /locus_tag="U2M88_002304"
     CDS             complement(6857..7207)
                     /locus_tag="U2M88_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEM8374653.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7240..8142)
                     /locus_tag="U2M88_002305"
     CDS             complement(7240..8142)
                     /locus_tag="U2M88_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEM8374654.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8135..8992)
                     /locus_tag="U2M88_002306"
     CDS             complement(8135..8992)
                     /locus_tag="U2M88_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEM8374655.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9326..10135
                     /locus_tag="U2M88_002307"
     CDS             9326..10135
                     /locus_tag="U2M88_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM8374656.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10175..10672
                     /locus_tag="U2M88_002308"
     CDS             10175..10672
                     /locus_tag="U2M88_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEM8374657.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11057..11130)
                     /locus_tag="U2M88_002309"
     tRNA            complement(11057..11130)
                     /locus_tag="U2M88_002309"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11094..11096),aa:Ile,seq:cat)
     gene            11317..11640
                     /locus_tag="U2M88_002310"
     CDS             11317..11640
                     /locus_tag="U2M88_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374658.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11804..12058
                     /locus_tag="U2M88_002311"
     CDS             11804..12058
                     /locus_tag="U2M88_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374659.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12128..12373
                     /locus_tag="U2M88_002312"
     CDS             12128..12373
                     /locus_tag="U2M88_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8374660.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12449..12814
                     /locus_tag="U2M88_002313"
     CDS             12449..12814
                     /locus_tag="U2M88_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374661.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12875..13438)
                     /locus_tag="U2M88_002314"
     CDS             complement(12875..13438)
                     /locus_tag="U2M88_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374662.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         4088 a         2577 c         2304 g         4976 t
ORIGIN      
        1 tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt
       61 tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa
      121 aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa cgattggcta
      181 tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat tcagttggac
      241 tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac aaaattatcg
      301 aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta tggtactttt
      361 tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac ttttgattta
      421 taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc
      481 ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat
      541 aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa
      601 cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc
      661 tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata
      721 taatcccagg catctaaact agcattatag aagagacaca catagaatct aaaataactg
      781 gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac cttcatactc
      841 tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag ttgttttaca
      901 ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa tgaaatttaa
      961 aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt tgttcggcgt
     1021 acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt ggcagaccac
     1081 attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg cttatttctt
     1141 attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa gcttaaacat
     1201 actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg gcttacttcc
     1261 taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg taggaatcgc
     1321 tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa aactgcttga
     1381 acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga catggcaatt
     1441 atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga attgagtgag
     1501 aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc aaaagccatc
     1561 cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg tgatttgttg
     1621 acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga acaaacagta
     1681 aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt acgtctatta
     1741 ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat gtactgactc
     1801 atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca actggtttcc
     1861 tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca ccagactgat
     1921 agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt tcataccttg
     1981 aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat tcttttttag
     2041 taagctccca tatatactgt aaagccttct atatattatg tcatgttgct ttttaacatt
     2101 tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct tttttatttt
     2161 taacattatt ttgataataa tgattattta tttaaaaata cattagataa catttgttac
     2221 gacacgaaaa agcttcacaa acaaatatcg ttattttaaa atgagggcga actcacacaa
     2281 ttaggaggaa tagtatgcca tttattgttt acttttttct ttcattactc gtgatctata
     2341 ttccattacc aacatttttc ctgctcgtca atcgtctagc agagcaacat gatacgattg
     2401 ctgctcttac gaaactttgt ttacttctat ttgtttgtct agattacttt acgagctatt
     2461 attggctaaa acagacaaga gtaaaaaaga gatattattt cttccttgtc tttttcaatc
     2521 tctttgagtt tctgatccat tgctttttac ttcatcagta tcatacgatc catttgtgga
     2581 tcatctgggt tttcaaagtg gtcgtattat tttgtatgtt cacctttcct atctcaagaa
     2641 aatttaggga ggcattgttt tattaaagaa gcttctctgt atcaataaag acacatttaa
     2701 ggaaaaactt ttaaaaatct aattgggaat ttgcccgcat aaataagagg ctgtgacaaa
     2761 agtcttgtca cagcctctta ttccgtataa acagtgttca aaagcttgca tcttcgataa
     2821 tatgcccaac caaatgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg
     2881 atgaagaaca gctttttcac gacctctttt taaaagtctt tttacagttt tcgagcaaag
     2941 aatgatacga ttagtactaa aatgatggca ccaatcaatg atggcacgat tgccattcct
     3001 gcaatacttg gtccccaaga acctaataat gcttgaccaa gtgctgatcc aactaaacct
     3061 gctacgacat ttgcaataat ccccatcgaa gaacctttat tcgtgattgc tcctgcaatt
     3121 gctccgataa ttcctccgac gataagtgac caaataaatc ccatgtttcg ttcctcctaa
     3181 tgttttactt ccttattact gaacccgtgg ttctgtattt tcttttactt tttgtgcgcc
     3241 gcttccagct gctgatttta ctttatctgt ttgattcgaa gcaaattcac cggttttttt
     3301 cgctgcgtca gttactttgt cttgcaccgt ttcttgatct ttttcaaatt cttcttttgt
     3361 tttgatgtcc actacgttag cgttgacttc gatcacttct aaatgtgtca tattttttac
     3421 ttctttgccg attacttctt gcatttcttc aaagattttt tcgatatctt tgccatattc
     3481 cacaatgaca tccatatcaa ctgctacttg ttttttaccg acttctgttt cgatcccagc
     3541 cgttacatta tctgtattca caagttttcc tgccacatta gagaagaaac caccgtttgc
     3601 agataataaa ccgtctactt gttcaattgc gattccgatg attttttgca caactttatc
     3661 ttcataattc agctcaccgc gcattgaatc ttcctgttca gaatcatgct ttccttggtt
     3721 atttgtatga tcagaaccaa gttttccttg tccgtttgtg tggtcagaac taagtttttc
     3781 ttgtgctttc gcatggtcag aaggatgttt ttgctcttcc acaggtttag aaggatcgat
     3841 tgcatcatgt tgttctggaa ctttagattc tggagttcct ggatgatgag gtgcatgctt
     3901 ttctgcatca tgtttcttct cttctactcc ttgttgaacg ttagggacta ctggatctgg
     3961 atgtgcagat actggtgctg ttttgttctt ttcatcgttc acaccatttg attggtttgg
     4021 tgtcgttggg tctggatgtg cagacactgg tttttggttg ttcattttat tgttattatt
     4081 tttgttattg ttattcatgg atgtttcctc ctaagattta aaatagtgat ctaagatttt
     4141 cgtttgtttc agataaaaac cgatagcgac gccgatcgct gccatgatca agatcaataa
     4201 ggtcttgaaa aagcccacgg tgatccataa aacggctaat atcaatccaa gtaccccaaa
     4261 gatgataggg agcttgtatg tcgaaaatag ttctttcatt tttggtcccc ccctattcta
     4321 ctcgtttgcg gtcattgctt cgtttagctg gttcttcaaa gcctgtgtat ttcaccgata
     4381 ctttcactgg ctcttttgtt cccaatatgt gttgtacttc attttcgatt tctttcatca
     4441 atgttcctgt tttaccaatg agtgctgaag ttcgtttcaa ctcccctttt acattgacgg
     4501 cgatcttgtt tttcgttgct ttgacattta cttttggtga atggatgaat tcatcttcat
     4561 gcaagtgaga acgaaccatt ccttcaattg cttttttatc caacgtcaac tccccatcac
     4621 catgtttcag tagaaagttt ccgattgttt ttggataaaa caggaaaaca agtaacgtga
     4681 taattgtcaa aatagcaaat gcaacggcaa gccaaaatac atattgttgc acgtatgaat
     4741 tagtgatgga aaagaatcga aacttctcta gctgaaaagg caatggatag gttgcactta
     4801 atagtatcgc aaacaaaagc acagatagca aaagtaatgt tccgatcact ccgatcgttt
     4861 ttacaccttt attcattttc gcacctcctc ataggggtga aacgatcaac ttcttataag
     4921 ttgatcgctt ctccaccagt tactccgtaa atttgggctg taacataact tgctttattc
     4981 gatgctaaga aaacataaac tggtgctaat tctcctggct gacctgcacg ttctagtaaa
     5041 ctttgctgac caaattcagg gatcgaatca gaaggttgtc cttcgtctag ttgtaatggt
     5101 gtccagattg gaccaggtgc tacaccattt acacggattc ctttaggtgc aagctgtttt
     5161 gctaaaccga tcgtaaagtt agcgattgaa gcttttgttg ctgcgtaatc taataaatgt
     5221 tcgcttggat tgaatgcttg aacagaagtt gttgttacga ttgctcctcc tgctgggata
     5281 tgcggaacag cttctttcac taaagcaaac atagcgatga tgtttatcat aaatgtgtct
     5341 ttgacttgct caagtggtaa ctcagcaata gactgacaag aaatttgctg tcctgcgttt
     5401 agtaccatcg tatctagccc gccaagttca ctcacggctt tatctatgat ttctttaggt
     5461 gcttgttcgt cacgaagatc aaaaggtagt aagacaatct tacgtcctgc atctttcacg
     5521 tattttgcta cttcctctgc atctttttct tctcctggga agaattgaag agcgatatct
     5581 gccccttctc tagcaaatgc aatcgctgct gcacgaccga tccctgaatc tccaccagtg
     5641 attagtacac ggcggttttc tagttgatta ttgccttcat aggattcttc tccacaatca
     5701 ggaacaggaa tcatattctg ctgtaaagct ggtgtatcct gatcttgttt aggaaatttc
     5761 tccgtatggt acaagtttcg cgggtcgatt aattttggtt ctgtcattgc tgtattcctc
     5821 ctagttttta aattgtattg ccaaaacaaa tttctttgtt tgatacctgt tttaaataga
     5881 ttctttaatt tgtataatct ttccggtata aatggtaacc taaaactact gctgctatca
     5941 aagagccaaa gactacgagt ataaagccta gagcagaact tttacccggt aatcctcctg
     6001 tattcattcc ccaaatccct gcgaacaacg ccggaacgga aatgattaat gcagctgaat
     6061 ctagatattt catgatatga tttagattat tgtccatcat cccgttgaat agatcaccaa
     6121 tactctctaa taaatctcga tagatctcga tcattttttc tgtttgtcgc tgtctcaaat
     6181 ggacatcata gagtagctgt tcatcgtcta aacgtttgat gaaagtttca gaattccata
     6241 attgatccat tgtttcccgt tgatctctta aggtatgatc gatatatacg atgtctcttt
     6301 caagatctgc ctgtttatac agttcttcat tttccgttgt tttacgagct gctttatcca
     6361 aagcgtcaat tcgctttttc atctctctta gttctttcac gtaatgggtg taaatatcta
     6421 ataatgtata cgtgatgatc ttttcaaacg agtttaaatg atttccatgc tttgtcagca
     6481 gccgatccac aaattcggtt tttctatcga tacaagtaat caagagatta tcggaaagta
     6541 caaaagatat tggcgttaat tttttttcga tacgattatc tgactgatct agattaatca
     6601 ctaccaaaga aaatggatgc tctagtttcg tctcataaag gcgctccatc cgagaaactt
     6661 cttccggata ttcggttcct acgaaaatat catcaggaag ctcgaattct tcgattgctt
     6721 gctcaatctc ttcgggcttt gcactatcaa gtacgagcca attatatttt ttttcatctg
     6781 tttctattac attcttttct tcatccacaa agtagtatct caatgtatac ctcctttttg
     6841 gactttctcg aaactattag ctgaagaagc agttctttaa tttgatatta taatcgagta
     6901 ctgcgatttc attttctaat gaaaagctgt caagctgttt gttgaagtcc atgttcaatc
     6961 gtttaccgaa ataattttgc tctaatttag atgtatagat gtccaataat ggatgctcga
     7021 ttttagtcgc atattctcca aattccgaaa caaaaggaat gatttgaaaa aagctgcctt
     7081 ttgcaaaagc acctttgtcc cttagaaatg gatcggaatc attgataagc gcgagtgcga
     7141 tttttgtatt ttccccaacc ttttcaatta acgtctctac tgcattttta gacattgtaa
     7201 tttccattcc tactccccct cttcagttgt tgtcttctat tatttaacaa ctacatctgt
     7261 ttttggttga acttttgcat attttggata tttagggaat ttagctacac aacggaaagc
     7321 cgtcatccct gcatattttt cgtaatattt cttcgttggc ccttcaaaat ccttgctcca
     7381 cggtttcaca tgtcctgaaa aatggataat gtaaggattg ttgcgagttt cttcgtattc
     7441 acgttcacct gctgctgttg gatgtttttt tgcttttgcc atgatatagc cttgtgcatt
     7501 ccaacgcgga tgaagtggta accaacggtc atgtaagata gcatttaatg catcttggtc
     7561 atggaatcgt aatttttctg gattatgttc gatgaaatct aatacttttt gcgtaatatt
     7621 ttcatctaac cattttttca cattgatcag catcaaacct gagttgaaat aacgcattga
     7681 tttagctgaa attttcattt tttccagtcg ttgatggaac ccagcgtctt caacagctgc
     7741 tacgattgaa tcaccgaagt caagacgcca taatttagag atatcttgta aagcgatcat
     7801 gtcgcaatcc atatataaga tacgttcaac ttctgtgccg cggaacaatt ccggaatcgc
     7861 aatacgataa taagctgttt ctggaatacg atcgctgatt accacatttg taaagaattc
     7921 tttattgatt ttcaagaatt ccacatctga gttcattcgt gcgtttttca ctgagaagcg
     7981 taaaagctgt ttactttcaa aatcgatatc atcgtcaatc acatagaacc gaacaaattt
     8041 agacgggttg caattatcta atactgagac aaataatgca gctaaatgtg gtacgaattt
     8101 tgtgttacaa cttgcgacta ctgctatttc ttttttattc attgatctcg cctactttcg
     8161 ttagagtact gtgtgctaat ttttttaagt ataaattggt atacggatga tctttcaacg
     8221 tattccaagg tttatcatgt cctgtaaagt gaacgatcga aggtttttca tttccttctt
     8281 tatactgacg ttcatatttt tcatttggtg ctggatgacg ttcaaaaatc agcgaagtct
     8341 gcatgttcca ttttggatga agttgttccc aatcttcata aagtacagca ttcaaagcat
     8401 cttggtcgtg atagatgatt cgatcgccat tttcactgag gtaatggata gttttttctg
     8461 taatttcttt tttattccat tgatcaatgt caatcaccat cactcctgaa ttgaagtaca
     8521 agtcatcgga atcgattcct aatctttcca aagctttcac ttggcctgga tcgataatag
     8581 cgccgattgt ttttccattt aacggctcat catatagttt tacgatatcg tctagtacta
     8641 acacatctga atctaagtaa aggactttct tataatcctc tttagctaat aagtttggta
     8701 aagaaattct taagtaagcg gtagttgtga tatggtcact gactaggaag tcttcgtaga
     8761 tatctttttc cactgtcagg aattggatac ttgcattact tgaatattta tttaccgttt
     8821 cttccaatcc ttctttacta tattctgata gaccatcgtc aatcacataa aattttatgc
     8881 gtcttgtttt attgcaattt tctaatgcag ttgcgatcat cacgctcaag taaggagcat
     8941 aattttcgtc tgaagcagtt accacaggaa ctacgccgta tctcttctcc atcaaaatca
     9001 ctcctttttc taatcttttt ctaacctgtt ctacatctag aatattcctc atttggtttt
     9061 aaagcaaaat atatgctttg ttttcattga atattttaaa atttttttat cacaaaacgc
     9121 atcggtaagc gctaactatt cgtttttcat ggctttttat tcaaaggtat tgaccaaaaa
     9181 ctactatata tagccacctt tttcttaaga atgttttttg aaaaaattca taatgacaaa
     9241 gcaagttagt ttcatttttt taaattctca tttaatctta aggtaacagg aaaatagata
     9301 tgtaaaaaga aagtaggaag ataaaatgaa actagcagcg attgatttag acggtacctt
     9361 gcttgatcga aatggaaaga taccagaaga aaatatccaa gcacttcgga aatttgatga
     9421 aaatggcgga atcgtaaccg tcgctaccgg aaggaacagc atttctgcta aagatatttt
     9481 ttccgaatta ggaatcggtg gctatttgat ttcttccaat ggttcactga ttgctgaaat
     9541 gaaggatgga aaaatggcga aagttctcag acgttcaaaa atagatagcg atacactgag
     9601 aaaagccttc ttctatgctc aagaagcgaa aatatccatc attgctagcc gagaaaccca
     9661 agatgatcag ctgacattcg atgaatcaaa tctagtcaaa gatgaccctt attatgaaca
     9721 tttcaattta cagaaccatt cttttgatca aattttaaat atgttggaag atccttctct
     9781 tagctatctt aaattagcat taacagataa gagtgaagaa aaactacaga agctacaaag
     9841 agatctgcaa gcagatggta tccaatcagt attctctgat ccgcatttct tagaaatcac
     9901 gcctacaggt atcactaaag ctcattcact tgtttttctg gcggattacc ttggaatcga
     9961 atcgagtgac atcatggctt ttggcgatca agaaaacgac attgctatgt tgaattacag
    10021 cgggttgagt attgcgatgg ggaatgcaca gcgacaagta aaagaaattg caaaccaagt
    10081 aacactgacg aatgaagaag ctggtgttgc ttatatttta aaaaacttct attaaaaaga
    10141 ataaaatgac gatatgaatg taggaggaaa gaaaatgtta gttattattg atatgcaaaa
    10201 tcatattctt gatccaaaca gcgaatttta tattccagag tcagatcaat tggcagaacg
    10261 tattgctcaa agattagcta aagcacgcca aaataatgaa tatattctct tcacacggga
    10321 tataccagtt gaactgaagg acaaagaaga agaacgagaa gatttgcaat tgatccctct
    10381 tctttctcca caagaaaatg aacgagtgat taaaaagtat tactttacga ttccacctga
    10441 aactttagct gaaatccagg aaactttatt tgaaagcaaa gaagaacaaa aagagatcga
    10501 agtagtcggg attgaaacaa acttgtgtgt actctctaat ctgataggcc tgcagagtgc
    10561 ttttcctgaa gcagactttt ttgttgatcc aatgttagtc tccagtagaa aacacggaga
    10621 atccgcttta gagctgttga aagatttcaa cgtctctatc gtcgaatcat aaaaatatct
    10681 atcttcattt acgatattta acacacaata aaaaaagcac gcagaactgt tttagtccta
    10741 cgtgcttttt ctatcaaagt agattggctt ttttatgaca cagagtgaaa cggcaggctc
    10801 acgacctctt tcttaaatgg tgttcaatca gctgaccctc ggtattagct taacaaacgg
    10861 caaaatatga ggaactattt ttccgtttgt taagctaata ctcaggtcaa aacgctgatt
    10921 tcacaacctc ttccttaaac ggtattcaaa agcttgcatc tgcagtaata agaacagcca
    10981 agtaaaaaca taagaagctg tttttacttg gctgttctta ttacttgatg cagggcagct
    11041 ttttcacaac ctcttatgta ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
    11101 gtctgctcta accaactgag ctaaaggtac atcataaata aaatttattt ttgttacttt
    11161 tcaactatag cacgaatgac aaactctcac aagtttcaaa atataagtaa aataatattg
    11221 ttattcttat ttcttttact tgtgtcataa ctattttgtt actttaattt ttatttgctt
    11281 attttagaaa aaatgttatt cttttattta ttctcaatgt taggagtgat tattttgcca
    11341 tttattgcga ttttattcga tttagttgcg gcagcagctt attttctcca attaaattat
    11401 caaacctcag gaattctgtt tattggaatg atttttcaag gtgtgattac ccttttgctt
    11461 cttgtcatga tgctcagcta taaaggaaaa agatatgccc gtattcaaac aaaagtattt
    11521 gttaaatatg tttccattcg ctatgggatc atcattcttt cctttttagt gaatgcgatc
    11581 gtcctctttt tatatgtatt gaattactta gatatcaatc cattgatttt ttctcgatag
    11641 ataagacaca ttcagcggaa cagttcctgc ttatcagcag aaaactgttc tgctttttca
    11701 tttttcattg atctattctt cttttatttt cctagcattt catttgtgat gtatgcaata
    11761 acatgttatc ttttattata tgaatcgttg aggagtccgt tacatgatta cgttggtgag
    11821 agttttgttt tgggtgcctg cggtcgcact ggtcgcaagt attgtttatc ttatgaattg
    11881 gaacaaagaa cggttttatt tagctatttt gactcttccg gctatctact ttatgtggaa
    11941 agtttttaat tacaactact ttgaacccga tagtgttttt atagaagaat tatcaggact
    12001 ggttttgtct ttattgatcg tcattcttta tttgatacgt ttaaataaaa aacattgagt
    12061 actattgatt gcatgtgtag ctttctatag tcattataac tattctacta taatgaaagg
    12121 tgattaaatg agtatcgaag aagctatttt actggatact ttacgttgtc gtctgaattt
    12181 cttaagaact ggccgatctt tatctcaagt agctgtttct agcgcgttaa gtattccttt
    12241 attcaaatat cgtaagtacg aagaaggaga agaaattcca agcatcattg aattaatcta
    12301 tatcgctgac ttttacgata tttccgttga ttacctaatt ggtcgctcag aaacagcaag
    12361 caaactgaag tgattttttc cagccttggt gctggttttt tcatttaacg tctaaggtta
    12421 acaatctata tataaaggag acatttctat gcgattatgg catgaaaaat tgatttcaaa
    12481 gcttcctcgt caacagcttt taggacaaca tcgggaatgc tgtgcattaa gaggaaatgg
    12541 ctggggaaag aaacatgcta ctgtggatta tgtttttcac tattctcctt atagattatt
    12601 ccaatatcat cagcttgtca tggatgaaat ggaaaaacgt ggttacagcc cagcaccaga
    12661 gtggaaaaat cctatgtatc gtggcaaatc atgcgaagca ttttttgatt tacctactgt
    12721 agagcgcaac gtgccgattt atccggaaca cgatgaagtt tatctagcag aatgtttagc
    12781 aaatttggag caaaaaggaa tttatcttac ctaatataac tagaaaagcc ttctatctcc
    12841 tcttacttca ttaaaaagct ttaaccatct tagatcaagc taaaatgtcg taagttttag
    12901 ggttatcttc atcctcacgt gcaaaactaa aagcgtactc tagttttggt tcatcgccaa
    12961 cttccctaga cataaatgaa catctcctga cgtggtttaa aagtaccgca tcttcgacgt
    13021 ctgtatacga tgcacagcac agattgcttc ttttgatctt tcttttgatt tttctgttgc
    13081 ttcttctaca ccttcttcga ttactacatc gatgcttaca attgcttctg cctaatattc
    13141 ccatgcatcg tcgatttaag cctctgtagt cactgtgatc gtttccacat tttaactcct
    13201 ctgtcgtggc ttcagttgat tttgtgatca gttcagttgc taagccagag tcagaacaac
    13261 ctgccattgc tagcgttaat attgatagta aaaatagttt ttttatcata aggttccccc
    13321 cctgaaaata gactaaaata ataacgcttt ctttttttgt tttcagacga aaattaataa
    13381 tttctaaata ttctttaaaa aagcttataa ctattatgcc ttttttaata tataacacag
    13441 ttatttttaa aaataagaat gctaaatcac tctacaataa cggcgaaagc acaaaaaata
    13501 aaaaaattat tacgttatta aaaaaacaga tgaaagatca ggttttatta gatctcattc
    13561 tttcatcact tgaagggatt taatgaacta ctttttcctc tttgtcgttt ttatatattc
    13621 gtattccttc aaacagtagg atagcctctt tcaatgcttg atatttgaac tgtccgttac
    13681 ttaaaaattt agatgtgccg ccctcttcct agaacatatt cttttttact ttttcattaa
    13741 ttgacagacc aatcgaacga aactgtaaat ttcgatagga taatgattgg tcaaactcat
    13801 tcattttcaa gacatctcga ttcatattca agataggaat ctttttatca acatcacttt
    13861 attcgtattt tcctctacca taaaagcttc acgctccaca aaattgtttt atacactact
    13921 attcagccca ttcaacgacc aatga
//
LOCUS       DASHRQ010000072        13428 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.076, whole genome shotgun sequence.
ACCESSION   DASHRQ010000072 DASHRQ010000000
VERSION     DASHRQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.076"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(76..2010)
                     /locus_tag="U2M88_002315"
     CDS             complement(76..2010)
                     /locus_tag="U2M88_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEM8374663.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            2291..3247
                     /locus_tag="U2M88_002316"
     CDS             2291..3247
                     /locus_tag="U2M88_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEM8374664.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3337..4440
                     /locus_tag="U2M88_002317"
     CDS             3337..4440
                     /locus_tag="U2M88_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEM8374665.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            4453..5406
                     /gene="menA"
                     /locus_tag="U2M88_002318"
     CDS             4453..5406
                     /gene="menA"
                     /locus_tag="U2M88_002318"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEM8374666.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(6220..7566)
                     /gene="gor"
                     /locus_tag="U2M88_002319"
     CDS             complement(6220..7566)
                     /gene="gor"
                     /locus_tag="U2M88_002319"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEM8374667.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(7700..8953)
                     /locus_tag="U2M88_002320"
     CDS             complement(7700..8953)
                     /locus_tag="U2M88_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEM8374668.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(8959..9882)
                     /locus_tag="U2M88_002321"
     CDS             complement(8959..9882)
                     /locus_tag="U2M88_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEM8374669.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(10103..12595)
                     /locus_tag="U2M88_002322"
     CDS             complement(10103..12595)
                     /locus_tag="U2M88_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEM8374670.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(12602..13069)
                     /locus_tag="U2M88_002323"
     CDS             complement(12602..13069)
                     /locus_tag="U2M88_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEM8374671.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(13205..13278)
                     /locus_tag="U2M88_002324"
     tRNA            complement(13205..13278)
                     /locus_tag="U2M88_002324"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13242..13244),aa:Arg,seq:acg)
     gene            complement(13336..13409)
                     /locus_tag="U2M88_002325"
     tRNA            complement(13336..13409)
                     /locus_tag="U2M88_002325"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13373..13375),aa:Pro,seq:tgg)
BASE COUNT         3943 a         2813 c         2360 g         4312 t
ORIGIN      
        1 aaaaaggagg ctgtgacggt ttgtctcagc ccctttctat tgaaatcttg cattcaaata
       61 acgttcttta tctatttatt tcttgccgta ctgggaactt ggtctaccgt accaccaatt
      121 acctagtgta cgttgtagcc atggaattac ccagcgatcc aacccaatcg cacgtccgga
      181 accgttcatc aatgcgaaag caacaaaaat gaaccaaatg tttacccagt aaaacatgcc
      241 tgataagcag aacgtgatca ccaatacaat cgttgtcgca ctgcatagcc atgtgaataa
      301 tccagcaatc aatgctaatg ccaaggcaat ttcaaagaat accatgaatt tttggaagaa
      361 cattgctaca tcattgtttg gcatgatcac ttgcatgatc tttgtgaacc agtctggtga
      421 cttctcaatc acagccattg gttcttcacc gtatgcataa ctcaaaccaa aatgtgcagc
      481 ttgagtcgct gcttctccgg cacttgcccc tgctccgctg gcagctgtgg ttgcatcacc
      541 agcagcagaa gcaccactgg caacatctgt tacattgtat tcccatgtcc aagggaacac
      601 actgtttcca gtgaaccatg atccttcacc aaaccaagta cttggtttga acacttcacc
      661 attcccaatg atttttttgg aagcttcaac gaaccaaact aaaccataga aaatacgaag
      721 tggcacactc cataagacat tgccgtaacg agaagaatgt cctcgagtca cgttgcgatc
      781 atctttgata tggaaaaact catgcattaa atattggaac atataataac cagaacgaat
      841 atcaaagaag tatttcaaat tcacgatatg tttcattacg atagctaaaa atccgcttaa
      901 atggatctta tcaaataggt tagctacccc ccatttggca ccaatagaaa ccataaatcc
      961 ttgatagttt cctttaaatt tatgcttttc tccgcctttg atcgaagcca cgatattcgc
     1021 tgcggctgtg tgtcctgttt gttcagcagc ttgaacgatc tgcggtgtcg gtgttcctgg
     1081 gaattcttca taaaagacta aatcaccaat gatatagatg tttttatctt cgtagccttt
     1141 tgcctgcata tattcattgg cgatcaaccg ctgtccgcgt gctgattcta atccaaagtc
     1201 agcagcgtca gaagttcctt tgacaccagc tgtccaaatc agtgtgtgag tcggaatgtt
     1261 gcgtccgtct ttcaatttga tatgatcagc agccacttct acgattggtg cattcaataa
     1321 taattccacg ttgtgcttac gcaaatagcg ttcagctttt gcagcatcat ttcgatccaa
     1381 catattcaag atcgtcggca ttgcctctac gaccatcagt gtgatttcgt ttggatctaa
     1441 tttataatct ttggctaggc gatttttcca gtcgatcagt tccccaatca tttcaatacc
     1501 ggtaaatcca gaaccacaga caacgaaagt cagcatggct ttacgcagtt catcatctgg
     1561 ttccaatgca gcttttgcta ctgtcttctc aatatgttcc cgaatcctta cagaatcttc
     1621 aaatgaccaa agggtgaatc cgtgtgcttt gacaccaggt gtaccaaagt cattcggctc
     1681 tccgcccatt ccaaggatca gctgatcaaa aggataggaa cctaatttgg tcttgacgat
     1741 ttttcgttct ttatcgatac cgattacagt gtctgtgaca agttgtacat tcttttgacg
     1801 agcaaacaaa tgctgcagat cgtattggat tgcttctggt tctacacgtc cgccagctac
     1861 ttcatgcaac tcggtcatca tagtatggta cgaatgacga tcgatcagag taattgctac
     1921 atcttctttt ttcagtttct ttgccaagta ttttgtagct gaaacaccgg catatcctgc
     1981 tcctacaacg acaatatttt gctttgccat tgatcatccg ctccttatag actatctata
     2041 taatgaatat atgataattc ttttagtttg atacttatta cacaatctcc atatattata
     2101 tagatgttct gttcttttgt ctaactcctt ttggattttt ttcaaatttt aaaataaagc
     2161 gttttttcac gaaatttgct gaaagaaaac gtttgaaata ttacatgggt tatttatcaa
     2221 aaatagttga cttataggct aaaaagatta gaatgtataa tgaatcacaa agaaaaattg
     2281 tggactagaa atggaatggt ggggtattaa aatgaagaaa aaacgttttg taactggatt
     2341 tgccgtatta gcattttcag ctctagtgtt aggcgcttgc ggcgcagatg acaacggaag
     2401 ctcaaactct tcttctgaat catctacagc acaatcttcg actgcaaaaa gcacaacaga
     2461 atcatctgca aaagttgtag ctggcggaga tttgcaagac ggcacatata aattagaaga
     2521 aaaaaattat tcaaatggtt accgtgcagt ctttgaaatg gttgtaaaag acggcaaaat
     2581 cactgaatca aaatatgata acgtcaatga aaacggcgaa tcaaaaacaa aagatgcaga
     2641 atacaacaaa aacatggaag caaaatctgg cacaaaccca gaaaaataca ttcctgagtt
     2701 gaatgaccaa ttcttaaaag cacaaagcgc aagcggtgtg gaagtcgtaa ctggagctac
     2761 tcactcatct gaaagcttcc aaaactacgc acaacaattg atccaagctg ctcaagctgg
     2821 taacacagat acaatcgaaa tcgacaatgg tgctgatttg aaagacggca cttacaagct
     2881 tgaagaaaag aactactcta acggctaccg tgtgcaattc gaaatgacag ttgctggcgg
     2941 caaagtaact gaatcaaact ttgattatat cgacaaagac ggtaaatcaa aacaagatga
     3001 tacagaatat aatgaaaata tgaaagcaaa aagcggtaca gaaccaaaaa cttacatccc
     3061 tactctgaac gacgaattcg tcaaagcaat gggcgaagaa gatggctcac ctgctaatgt
     3121 agaagttgtt acaggtgcta cacacagctc acattcattc atcatgtacg cacaacaatt
     3181 agtgaatgca gctgaaaaag gcgacactca aacaatcgaa gtagataata tcgtaacaga
     3241 aaaataatca cataatcata ctaataaaag ttggagggtg ggacgatagc cagattaggc
     3301 agtcgtctca cctttcaaaa tgaagagagg aaaaagatgc ggaaaaaact actattttcc
     3361 ttaatcacac tgggcatctc ttttatggtg ctaactggct gcagcaactc agcaaataaa
     3421 acaaccactg aaagttcagc agctaccaag atattgaaag atccatattc tgatgaacag
     3481 ttcttgttag ggacttacgt tcgtatccgt gtgtatgatg aaggcaaaga atcagcattg
     3541 aaacctgcct tcgatcgagt aaaagaactt ggtgataaaa tcacgatcaa tcaaaaaggt
     3601 tctgagatcg atgaagtgaa tgagcaagct gggatcaaac cagtaaaagt ttctgatgac
     3661 gtctacacgt tagtcaagcg ggcctacgaa tatagccaag attctcaagg cggattcgat
     3721 atggcgattg gtgcaatcac acaattatgg cgaatcggtt ttgatgacgc cagaaaacct
     3781 tcccaagaag aaatcgatca ggcattgaaa ttagtggatt atcataagat cgaactaaat
     3841 gacaaagaaa aaactgttta tctaaaagaa aaaggaatga tcatcgattt aggtgcaatc
     3901 gccaaaggtt acattacgga cgaagtagtg aaagtcttga aaaaacaagg agtgaccact
     3961 gccatcgttg acttgggcgg taatgtctat gtcttaggcc acagcccaag aggcgaaaac
     4021 caagactgga cagtcggcat ccaagatccg aacatggctc gcggatctgt tttaggttct
     4081 attaaagaac ggaacaaaac attagttacc tctggaattt acgagcgtta tttagaagta
     4141 gatggcaaaa aataccatca tttgtttgat ccaaaaacgg gttatccgtt cgacaatgat
     4201 atcgcaagtg tcacgatcat aacagataaa tcaattgatg gtgacgggtt gtctacagca
     4261 gtcttttcaa tgggtgtgaa aaaaggactg gaatacgtcg aaagcgaatt gaataacggg
     4321 acggaagcga tcttcgtgac aaaagacgat aaagtatatg tgaccgatcc aatcaaagac
     4381 accttcaaat tatcggagga ctcccattac acgatgggtg accgttctga actaaaataa
     4441 ggagtacgaa ccatgacact aaaaatcttt ttagaagtag ttgaaatacg aacaaaggtg
     4501 gctagtgtat ttccttttgc ggtaggggta ttattctcta tcgctttttt tcatgagata
     4561 cattggctaa acactttttt attttttatt ggaatgctag tttttgattt agcgactacc
     4621 gctatcaata attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa
     4681 aatgtcattg gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg
     4741 attgcatttg tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt
     4801 ttgatgggat ttatctgttg ttttattgga gtattctata cattcggacc gattccttta
     4861 tctcgaatgc ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc
     4921 atggtcgttt atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca
     4981 tctggacaat ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc
     5041 ttacccttgg tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac
     5101 cgtgatattg aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca
     5161 gtcttattcc agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc
     5221 ggtatctatg aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat
     5281 ttggatcaat tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat
     5341 atggtcttat tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc
     5401 atttaacagc atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca
     5461 aataaaaata gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt
     5521 ttgaacaccg tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa
     5581 ttttgtgaaa atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc
     5641 ttttgaacac cgtttatcag aggttgtaaa atcagtgttt tgtcctgagt attagagaaa
     5701 caaatggaaa aatagttcct catattttgc cgttttttga gctaataccg aaggacagct
     5761 gattgaacac tatttgttcg gaaaaagtga ctaactatga cacaattttt ttcacagtcc
     5821 cttttttcac ttgatttgat tcgtaccagc gaacttgacg agaactttct tggtttggta
     5881 actatatggt attcttttaa catcctcgtt tgcttcttct aatttattta tacaaagtca
     5941 cactttttta aacatctgtt cttcgataca tttctatttc ttttaaacat cgataccaat
     6001 attttgaact taagagtgca taccaaatgg attcatttac tcttcgtcag ttacgaagat
     6061 aatcaaaatc tccaacttcc ctacttattt cgttttgaga attatcgaag caatcttaca
     6121 taaataacta aaaaacaaaa aaccagaaaa tcagtatact gattttctgg ttttacatgg
     6181 tttgttcaat aaaaagctgt tggaactgtt aatattaaat tatcgcatcg ttacgaattc
     6241 ttccgctcct gttggatgga tggctactgt attgtcaaag tcttctttag ttgcacccat
     6301 cttgatagcc acggcaaaac cttgcagcat ctcatcgaca ccaacaccaa tagcatgtaa
     6361 gccgacgatt ctttcttctt ttccgacaca gatcaatttc atttcgcatt tttggcgata
     6421 atcatttaat gcaaagtaca ttggggtaaa gcgtgaacga taaactttca attcattttc
     6481 gccatatttt tccaatgctt cttcttctgt caaaccaatt gttgcaattg gtggatgcgt
     6541 aaagacgact gtcggaacaa gtttatagtc aagataagaa tctttctttc cattaaataa
     6601 acgttctgat aaacgtctac cagctgcgat tgcaacagga gtcagatcta atttaccgat
     6661 cacatcacca acagcaaaaa tatgttcatc cgttgtattt tggtatttat ctactttgat
     6721 aaaccctttt ttgtccaatt ccacatttgt attttccaga cctaattttt cggtattcgg
     6781 tacacgtcca ccggcaaaaa gtacacaatc accagttaag gtttcaccat tttcaaatag
     6841 caccgtatat tcgtcattgt ttttagtaat ttctttagca atatgatgtg cgtaagtttt
     6901 tattccagct tcttggtaca tttggatcaa attgtccgaa agcattttat caaacgttct
     6961 aagtggtcgt tcttttcgat atgcccacat gacttcacta cctaaaccac gaaatacacc
     7021 tgaaagttca gccgcaatgt atccagcacc taaaacgatt gctctcttag gtaactctgt
     7081 taattcaaaa aatccatttg aatcaattgc gtattctcca ccaggaatgg tcattttact
     7141 tgcacggcct ccagtagcga tcaagatttt aggagcacga taggtttcac cgtttacttc
     7201 gatcgtttga ctatcgataa aagtagcgta accttttata gcctcgacat gattgctatc
     7261 taatcctctt tgataagcac cgtgtagaaa atcaatatat ttttctcgtc tttctactag
     7321 accggcgaaa tcaacatttt ttaaatctgc ttggataccg taacttgagg catctcgttc
     7381 aattgtttcc aaaatcgtac ttgcttgcca cataactttt ttaggtacac aaccgacgtt
     7441 gacacatgtt ccgcccaatt cattcgcttc aatcagcaaa acttttgcgc catgcatccc
     7501 tgctctgttt gctgaagcaa taccgccaga gccgccacca attacgatgt aatcatatat
     7561 ttgcattttg ttcctcctta ttgaagagat caatcaaaac ctttatttaa cgatcctttt
     7621 tctacagaaa acaagtgtaa cacttttctt gaattttgta tgcgaagatg cttaaaacag
     7681 ggatatatca gctttttcct tatttacgtt ttcggatcat atctccaggt ttcaccggct
     7741 gtgcgactgg catagttaaa atcatcattg gatttggtgc tcggtcaatg gattcgcctt
     7801 cttcattatg cataacttca acggtttgac tgaaatgact gaatcctgga ccataaaatt
     7861 cgatttcatc tcctacagaa aacaagtttc tctgacgtat cgtagcaatc tgtgttgcct
     7921 catcataaga taatacttca ccgacaaatt tatatactgg gattttacga cgttcaccga
     7981 ataattgttc gttttcagtt ggtgtattgt agtaaaaacc tgtagctaat tctcgttgtg
     8041 ctactttcca taactcgtcg atccattctt gcttgcagac atagttttca ggatcttcca
     8101 tataactgtc tacagccgct ttatagacat ttgacaccgt agatacataa tggatcgatt
     8161 tcattcttcc ttcgatcttg aagctgtcta cgccgttttc aatcaattct ggaatatgtt
     8221 cgatcattga catatccact gcactcatcg agaattcttc ttccacttcg cctgtatcag
     8281 ttaaactgtt tcgttcttga ccaaaaggca tatcgaacaa tccatacttc cagcgacaag
     8341 attgagaaca tccgccacgg tttgcatccc gcatcgacat atggttggac aatgtgcatc
     8401 gaccagaata agagatacac atggcaccat ggataaatgc ttcgatttcg gtatccgtat
     8461 ttttacgaat ctctgccact tcctccattg aaacttcacg agctaaaacg acacgttcca
     8521 acccttcttc tttccagaat tccaatgtct caaaattagt tgctgaagct tgagtggata
     8581 aatggatcgg cagacctggg gcttctgtcg cacaaatttc gatcaaagct ggatcagaaa
     8641 caatcactgc agaaataccg acatcacgca aactgcggaa aaattcccct gctccatctt
     8701 catttccttc atgggtgacc atattagcag caacgtatac cttcgcatca tgttcttttg
     8761 caaaagcaac tccttcagcc atttcttcat aagtaaaatt acctgcacgg cttcttaaac
     8821 catatgcatt cccgccaata tatactgcat ctgctccata atggatcgcg gtttttaact
     8881 tttccagtgt tcctgctggc gctaggattt ctggtcgttt caattttctt tttactgtca
     8941 tttctttcat catactcctt atttgatagc agaaggatca atataaaaga atcctgtatc
     9001 taattcacgg ttttcaggat gcaatttact gatttcgcta gtcatttttt ccgctttttc
     9061 ttgatcccag ctaccttcta aaatttgttt ttttgcttcg gcgaatagtc gagtgatgtt
     9121 tacgaatttt tcgcctggag taaataatcc gtccaatttc catgtacagt aatgatgctg
     9181 ataaagttca ttcaattcat tcgagagatt cagatcattg ctggcaaaaa tatgtgtgcc
     9241 atgactatct tcataaatag aataatgtgt ctcttctttt ttaggttccg agataaaaag
     9301 acctctttca cgatcttttt gttcgtcttg tttcgtatag ttatagtaat tctgtagcag
     9361 tggacgtttg gattgatgga tgcatgtagc accatacaca agggtttcta caggaatatc
     9421 gagtttttct tccatcgcct tcatctcttc aaaaggaact tcacgagcaa gtactgctcc
     9481 aactgcgcct tttttcgccc agaaattgat ttgacgtgcg ctcgttacta atgtttcagc
     9541 atcataaata tacggtatac gcaattcagg attttttttc atcgtataaa taatacctgt
     9601 atcaccaact gtgatcacat ctacgtttat ttcttttaaa aaagccaaat attcaggtac
     9661 atttttcatt ttttcaggat gaatgatccc atttacagca atattcgctt ttttaccata
     9721 cttgtgtgct aattctacca attgtcgctg ttcttctcgt gtaaatgaag caggcagacg
     9781 caatccaaat tgctcttcac cgaaatatag tgtatctgca cctgcagcaa gcaactgttc
     9841 tgcttgttca atagtttcta ctgtggttat gagttcgatc ataattttct cctcctacaa
     9901 cttcataata gtagcatagc tctaaaaact ttccaatact tcttctattt aaagtcggat
     9961 ttttgaaaat tatttcacaa aaaatcaaaa taaagttcga tatattctgt ccaaatataa
    10021 aaagagattg tgacaaaaat cttgtcacaa cctcttaatc aagtcttact attataaaaa
    10081 ttcagctaca caggccaagt aactataggg aacctactgt ttccttttta ggtccgcgaa
    10141 cattcaatgt gatcttacct tttgaagcac ctactgttac gtggtcacct aaacgaattt
    10201 ctccgccaag caaagcctcg ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga
    10261 ttggacgtgc accatattca ggatcaaatc cagctttccc gatcacatcg attgcagatg
    10321 gtgtgatttt cacttgaata tcttgttctt tcaatcgttt gataatcgct ttgctcatga
    10381 tttggacgat tgtatggatt tcatctttac tcaatgaatg gaagacgaca gtctcatcga
    10441 ttcgattcaa gaattcaggt cggaaagctt tctttaactc ttctaagata cgtttttgca
    10501 tggctttgtg atctttagta acatcttgga cattgaatcc gacatttttt tcttctcgaa
    10561 tttgtgtcgc tccgatattt gaagtcataa tcatgattgt atttctaaag tccacttttc
    10621 tacctttaga atctgttaaa tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt
    10681 ctggatgtgc tttctccact tcatctaaga gaatcactga atatggtttt tgacgaattt
    10741 tttctgtcaa ctgtccacct tcatcatacc ctacgtatcc tggaggagac ccgattaatc
    10801 ggcttgtact gtatttttcc ataaattctg acatatcaac acggatcagg gcatcttcac
    10861 ttccaaacat gacttctgaa agtgctttgg ccaattctgt ttttccgaca ccagttggtc
    10921 caaggaacat gaatgaacca attggacgat caggatcttt caatccgctt cgtgcacggc
    10981 ggatcgcacg tgaaactgct ttgacagcct catcttgacc aacgacacgt tcatgcagaa
    11041 gtccttccag ttctaacaag cgttcgcttt cttttttctc caactgctgt agagggacac
    11101 cagtccactc cgatacaact gtagcaacat cttcttctgt tacacgatca gaataacctt
    11161 gctcctgttt tgcttcaatt gcagaaactt tttgcagctt tttacgtagt gctttttctt
    11221 gtctgcgtaa ttgagcagca ttttcaaagt cttgtttttg gatcgctgct tccttttctt
    11281 gtactagttc atcgatttct agtttgatca cagttgattt tgttaagtga tcggtttgat
    11341 ccaaacggac tttcgctgct gattcatcga tcaaatcaat tgctttatcg ggtagttgac
    11401 gagaagtgat ataacgaaca gaaagattga ctgctgctcg tacagcttca tctgtgatct
    11461 ccactccatg gtgctcttca tatcttgtac gtaatccttg aagaatcact tctgcttctt
    11521 caggagttgg ctcatctact tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt
    11581 cgatatattt ttgatattca tctagggtcg ttgctccgat cgtttgtaat tctcctcgag
    11641 ctaacgccgg tttcaagata ttcgaagcat caattgctcc ttctgctcca cctgcaccaa
    11701 ttaaggtatg caattcatca atgaataaga ttacttgacc atcattatag atttcgtcga
    11761 tgaccttttt cattcgatct tcaaattcac cgcgatattt cgtaccagca accaaagatc
    11821 ccatatcaag catcatcagg cgcttttctt gcatgtcttc tggcacttca cctaaaatga
    11881 ttttttgagc taacccttca gcaatggctg ttttgcctac accaggttca cccaccagta
    11941 cagggttatt ctttgtccgt cggctaagaa tctggataag ccgtttcact tctctcgaac
    12001 gaccaacaac tgggtccagc ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg
    12061 aatctagcgt aggtgttcct tgcggtgttt gccgtttatc ttgtacattt acatttctac
    12121 gacgattcat tccttttcca gcagaatcac cagctagccc cattttttgt ttcagaactt
    12181 gacgggcttt tgaaaggctg atacctaagt tgatcatgat tcgagtagcc atgatacttt
    12241 catcttttaa taagctcatc aaaatatgtt ctgttccaac tttttgagaa ccagttttct
    12301 tcacttcgct gctagctaaa gcaaaaactt gtttcattct tggtgaatat ggaagaaaga
    12361 ctcctttagg gtaagaacgg ataccaccat aaccaatcag atgttcaatt tcttcataaa
    12421 tttccgtttc attcaaatcc atttcacgta atacttttcc agcaattcca ttttgctcga
    12481 ccactaaggc tagtaaaaca tgttctgaac taactgattg atgtttcaac cgttttgctt
    12541 cttcttgtgc aatttctaat actgcttggg ctctttcagt aaaaagttct tccatctaat
    12601 ctcacttccc atcttcataa cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg
    12661 tatgatcttc atattcattg aaattcaatg tgttttttgc aattgcactc aacatcaagt
    12721 ttccttcttt ttttgtaatt atttggtctt cataaagttt ttgaataata gcaaaagcat
    12781 ttttctcact gattgtatca tcaaataact gatttatctg ctcaagtaat tgttttttat
    12841 ctgaaatcct tacttttgca attcgtatat aaccaccacc accacgtttg ctttctacta
    12901 gataaccacg ttggatggta aaacgggtat taatcacata gttgatttgt gaaggaacac
    12961 agttaaagag gtctgccatt tcaatccttc gaatctcgat catttcacta ttttccagaa
    13021 ttttctttat atatgattca attaaatccg aggtattctg atgtgccatc ttattcattc
    13081 cctttgacta atattgacct taattataac gaatttgata atagaaagaa agaagaacgc
    13141 tttttgttaa ataaatcctt tagaatcaac aaaaaaaaat acggaatata ctattccgta
    13201 tcgacgcgcc caagaggagt cgaaccccta accttttgat ccgtagtcaa acactctatc
    13261 cagttgagct atgggcgcat attcaattta aaaaaattat aaatcatttg aaaacaaaaa
    13321 gcaagataaa aaaaatcggg aagacaggat tcgaacctgc gaccccttgg tcccaaacca
    13381 agtgctctac caagctgagc tacttcccgt ttaaaaaata aaaaagac
//
LOCUS       DASHRQ010000073        13145 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.077, whole genome shotgun sequence.
ACCESSION   DASHRQ010000073 DASHRQ010000000
VERSION     DASHRQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.077"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(77..149)
                     /locus_tag="U2M88_002326"
     tRNA            complement(77..149)
                     /locus_tag="U2M88_002326"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="U2M88_002327"
     CDS             530..1099
                     /locus_tag="U2M88_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEM8374672.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="U2M88_002328"
     CDS             1086..1946
                     /locus_tag="U2M88_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEM8374673.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="U2M88_002329"
     CDS             2136..2840
                     /locus_tag="U2M88_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8374674.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="U2M88_002330"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="U2M88_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEM8374675.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="U2M88_002331"
     CDS             4677..5993
                     /locus_tag="U2M88_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374676.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="U2M88_002332"
     CDS             5994..6863
                     /locus_tag="U2M88_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEM8374677.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="U2M88_002333"
     CDS             6918..7727
                     /locus_tag="U2M88_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEM8374678.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="U2M88_002334"
     CDS             complement(7830..8459)
                     /locus_tag="U2M88_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEM8374679.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="U2M88_002335"
     CDS             8629..9921
                     /locus_tag="U2M88_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM8374680.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="U2M88_002336"
     CDS             complement(9997..10659)
                     /locus_tag="U2M88_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEM8374681.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /gene="groES"
                     /locus_tag="U2M88_002337"
     CDS             10884..11168
                     /gene="groES"
                     /locus_tag="U2M88_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEM8374682.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="U2M88_002338"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="U2M88_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEM8374683.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4369 a         2264 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccatt
//
LOCUS       DASHRQ010000074        12720 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.078, whole genome shotgun sequence.
ACCESSION   DASHRQ010000074 DASHRQ010000000
VERSION     DASHRQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.078"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(97..276)
                     /gene="rpmF"
                     /locus_tag="U2M88_002339"
     CDS             complement(97..276)
                     /gene="rpmF"
                     /locus_tag="U2M88_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEM8374684.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(334..903)
                     /locus_tag="U2M88_002340"
     CDS             complement(334..903)
                     /locus_tag="U2M88_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEM8374685.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1089..1991
                     /locus_tag="U2M88_002341"
     CDS             1089..1991
                     /locus_tag="U2M88_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEM8374686.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2026..3810)
                     /gene="pyk"
                     /locus_tag="U2M88_002342"
     CDS             complement(2026..3810)
                     /gene="pyk"
                     /locus_tag="U2M88_002342"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEM8374687.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3884..4846)
                     /gene="pfkA"
                     /locus_tag="U2M88_002343"
     CDS             complement(3884..4846)
                     /gene="pfkA"
                     /locus_tag="U2M88_002343"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEM8374688.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5014..8328)
                     /gene="dnaE"
                     /locus_tag="U2M88_002344"
     CDS             complement(5014..8328)
                     /gene="dnaE"
                     /locus_tag="U2M88_002344"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEM8374689.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8559..8744
                     /locus_tag="U2M88_002345"
     CDS             8559..8744
                     /locus_tag="U2M88_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEM8374690.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8806..10002)
                     /locus_tag="U2M88_002346"
     CDS             complement(8806..10002)
                     /locus_tag="U2M88_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEM8374691.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10172..11608)
                     /locus_tag="U2M88_002347"
     CDS             complement(10172..11608)
                     /locus_tag="U2M88_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEM8374692.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11901..12710
                     /locus_tag="U2M88_002348"
     CDS             11901..12710
                     /locus_tag="U2M88_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM8374693.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3797 a         2675 c         2247 g         4001 t
ORIGIN      
        1 acaactcatc agtccttatt gacaaagagc ccaaaaaaat aaacctcaag gctccatggt
       61 tagccttgag gttttacatt ttctaattaa aatgtattaa gcttcttttg acattacgtc
      121 cttaccatca tagtaaccac aatttgcaca tacgtgatgg ctttttttca tttcaccaca
      181 gtttggacat tcgtttaacc ctttgattgt caatttgtag tgtgtacgac gttttgcttt
      241 tttagctttt gatgttcttc tagctggtac tgccattttg ctacacctcc ttgtaaagtc
      301 ttgtatctaa attagacaca atatattcca atcttacttg ttatcgtcgt cttttgattt
      361 agaatcaaaa agttcagata gttttgcaag acgaggatca attttattct cggcttcttc
      421 catttttctg cgctggtatt cttcttcgga aacaacttcc caatcattgc cttttggaag
      481 ttctgttgct gcttcctctt cttttgtcaa tacccgcatt ggaatcgcca ataagatgtt
      541 gtcttcaatc gactctgcta aattcaataa tggaccttca attagcaaaa tctcatctgc
      601 agacatttgt tcatctctac tttggaattg ttcttctgtc atgaatactt cgtctacaga
      661 aaaatccatg ggcagttcaa caggttctag tgaacgtgtt gaaggaactg tcaaagtcgt
      721 ctgcacagtg taatgaacga tatagccttt cttatcaact gagaccaaac cttcgacttc
      781 cactggcgca acatcaagta ttaaattgtc tcgcttcatg agagcggctt tcaagtcgag
      841 cgtctcatga aaagttaacg gtgtctcctg gtattttctc aattccaata aagaccattt
      901 catttttcat cactcctaag caacaaaagc aattatacag ggcaaaaagc atgttgtcaa
      961 tgaaattttc ttgacacctt gcagttcata aaaaaacaga aaaatcttta ttagaaagcc
     1021 tctgtttgct atcatcctca gaatcattta caataatcat tattccatta aaggagcgga
     1081 tgatcttcat gaacacgaaa gatatcgaat actatgtcca aatcgtcaaa caaaaaaatt
     1141 ttacgaaagt ggctaattat ttccgtgtga gccaacctac tattacatat gcattacaac
     1201 gtcttgaaaa agagtttgac accgtactta ttacaagaga tcgatcgcat cacgaattaa
     1261 tcgtcacgcc ttctggaaag cagctgtttc tccatgcaca aaccattctc caagagattt
     1321 ctactgcaag agctgaaatc actcagttgc aaaagaaaaa gcttcgtttt ggcatgcctc
     1381 ctattatcgg caatgaatac tttcctaaac tgtcaagtta ctttatcaag catgaattga
     1441 tgaatcaaat tgagattacc gatggaggct ctcgggactt atacgggatg atccgacaag
     1501 ggaggatcga tttagctgta ttaggctcaa cacagccaat cgtggatcaa tatttagaaa
     1561 cagagttact tttggacaaa cgtttcatga tcgtcgtgcc acctacacat cctctttcca
     1621 agcaggatac tgtctctttc aaagatatag cagatgaaca gtttgtacta ttgaacgaac
     1681 attatgtcca ccccgcagca ttcactaagc tttctaaaca aacacatact catccaaaag
     1741 ttgtttatca aagcaatgac ttgacgattt taaagagtat ggtccgagaa aatgtgggta
     1801 tcggtttttt ggcagagatt gctgtccatc ctgaagatca tttagtttgc ctccctcttt
     1861 cagatgaagt tcagcctcgc ttcttgattt cattggcaaa acgaacacag cagatcgtca
     1921 cgcctcttca agaacaagta gtccaagtta tccatgaatt cgttacagca caaaaaaaca
     1981 gctcttcgta aaagaagagc tgtttttatt ttttattaag ctaaattaga tagctgttgt
     2041 ttcgccacgg taaacgatgc cgcgacgtga atcaacggta atcaattcac cttctgtaat
     2101 tgttgttgtt gcgcctttag caccaacgat tacaggaata tcttgtgcaa ttcctacaac
     2161 tgcagcgtga gaagtcaatc cgccttcttc aacgataaca gcagctgctt tttcgattgc
     2221 aggcatatat tctttgtcag ttgatggtac aactagaacc attccgtctt ttgctttttt
     2281 tacagcttct tcagcagatg aagcaataac tgcatttgca acgacagatt tttgtccaac
     2341 gccttgagct tctaataatt tagaaccaat catttgaatt ttcatcacgt tagttgttcc
     2401 gcgttcacca actggtacac cagcagtgat caagattaaa tcgccttctg atgcgaaacc
     2461 taattcaaca gcttttttag ctgctagttc aaacatttca tcagttgttg atggtttttc
     2521 agcaactgtt gggtaaacgc cccagtttaa catcaaacca cgttttgtgc gttcgtcaaa
     2581 agtaacagcc aagatatctg catctggacg atattttgaa atcatttttg cagtgtatcc
     2641 tgattcagtt gcagcaacga ttgttttcac gcctagattt tttgcagcac gagctacaga
     2701 taaaccgatt gtttcagtta catctgtttt atcgaattcg ttcaattgga atgtacctaa
     2761 ttctgataat gcattttcag cttcttggtc gatacgtgcc attgttgaaa cagcttcaac
     2821 tgggtagtcc ccgtttgcag attcaccaga tagcattgtt gcatctgtgc catcaaatac
     2881 agcgttagca acgtcagaag cttccgcacg tgttggacgt gggttttctt gcattgattc
     2941 taacatttgt gtagcagtga taactgggat accagcagca ttacattttt tgataatgcg
     3001 tttttgaacc attggtacta gttcagctgg gatttcaaca cccatatctc cgcgggcaat
     3061 cataacaccg tcagaaactt tgatgatttc gtcaatgttg tcgatacctt cttgagattc
     3121 gattttaggg aagatttgaa catgagtcat gtctttttct tctaagattt cgcggatatc
     3181 tagaacgtct tgtgctttac gtacaaaact tgcagcgata aagtcgatat cgttgtctaa
     3241 accaaaacga atatcgtcag catcttttgg agtaatacct ggcaaactaa tagaaacgcc
     3301 tggtgcattt acccctttac gtgatcctag catacctgca tttttaactt ctacgactaa
     3361 ttcacggttt gcttcgtctt tttcaatgat ttgcatatcg atcaaaccat catcaaataa
     3421 aatgtgtccg ccaacgtgaa catcgtcaaa taatcctggg taagtaaccg cgattttttc
     3481 ttttgaacct acatgttcag gatccataga gatgcgtgtt tgatctccta cttcaaattt
     3541 gatgtatcct gcacgaccaa aatcagcttc tgttgtgcct tgaacagttg tacggatttc
     3601 agctcctttt gtatctaaaa gaataccgac atcttttcct gtctttttaa ctgcttcacg
     3661 gaccatagtc atacgtgata attgttcttc atggtcacca tgagaaaaat taaagcggaa
     3721 gacatttgcc ccagattcaa tcaattgaga aataatttct actgagttac ttgctggtcc
     3781 taatgtacta acgattttgg tttttttcat ttaaaaaact ctcccattca attttgttta
     3841 tttaaagctg tacacagctt aaatagcttg gtttctttca ctcttaaaat gaaagtgaat
     3901 tatttagatc atatagtgat aagtctggtt tatgtttgtt gttttcaagt gtatcaatga
     3961 tgtcagacgc aacgatttcg ttgtcatgga taccgacaca taatccacct tttccttctt
     4021 gaagcaattc gacagcgtaa gcaccgaatt tacttgccaa cacgcggtcg cgtgcgcttg
     4081 gtgctccacc acggacaacg tgtcccaaga tagatacacg tgtatgatag tcaccaaatt
     4141 ctgaaagttt ctcagcgaat tcatttccgc ccattacacc ttcagccaag atgatcaagc
     4201 aatgtttctt cccgcgatcg cggccttctt ggattttttt agcaactgaa gccatatcaa
     4261 aatcatgttc tgggatgatg atttcatccg ctccgccagc cacacctgac cataaagcga
     4321 tgtctcctgc attgcgtccc attacttcga taacgaatgt acgaacatga gaagtcgctg
     4381 tgtcacgaat acggtcaatt gattctagta ctgtattgat tgctgtatca aaaccgattg
     4441 tgaaatcagt cccaggaata tcattatcga ttgttccagg aatacctact gctgggaaac
     4501 cgcgttttgt caaagccatt gcgccatgat aagagccgtc tcctccgata accactagac
     4561 cttcgattcc gaattttttc aattgttcga ttccttttaa ttgaccttct tcagttgcaa
     4621 attctggata acgtgcagaa tacaagaatg taccaccacg ttggatcttg tcacctacat
     4681 ctgccacatc aagacgacga atatctccag caactaaacc ggcaaaacca taattgatcc
     4741 cgtatacttc cagtccttcg taaatacctt tacgaacaac ggcacgaaca gctgcattca
     4801 ttccaggtgc gtctccgcca ctggtcaaaa ttccgatgcg tttcataata aacattcacc
     4861 tcattaaaat actttatcct aagtatgact taggtttaca accatttttg cgttaaccac
     4921 gactaacatt ctaacattat ttttctcact tgtcttgatt tacggataaa aaaatgaaaa
     4981 aaaatgaaag caagtagtat tttttaccgt aagttatttg aaaaccacat tatcttcgcc
     5041 aagcagataa ataagttgcc cctttatact cttgctgtca tccacccaat aagcatctga
     5101 cagaactact tttcgttgcg ttttttcgtg atacataact actggtatat atcctgggtg
     5161 tttctgaaaa atcacttgta attgtttcat tatctcggga tcttcccgtt cttctgtaat
     5221 acgcaaatag caagtttgtg aaggatactg ggcttctaac tggttaggat cagcaaagct
     5281 ttctccaatt agttgcagtt cctgattata tttgctgatt tcgctccgcc cttgaacaaa
     5341 gatcactcga tcaacggcaa acaaattacg atatttacga taaagagttg gaaaaactgt
     5401 gacagaaatt tctccactac tgtcggttcc ttcaacaaag gccatcagtt ctcctttttt
     5461 tgtccgaatt tctctaactt ccttgatcat cactaataaa ttgactggtt gattagggac
     5521 gatatctgta atcagctgaa cttgtttagc taaacgaaga cgatcaaacc cttctgcagg
     5581 atgacctgac aaataaatac ctaagtactc ctcttccagc ttcaagcgtt cttctagtgt
     5641 atagtcagcg acttcttctt cttttaatgc catgattccc agcaagtcaa gacttccacc
     5701 actgtaaacg atattttgga tttttccttc gagatcaagc atcaattgtt tcctgttagg
     5761 agataattca tcaaaaactc ctactgctgt cagtgattgc agtaactcta atttcagcca
     5821 acgcttatcg atccgtatga ggaattgatc cactgactgg aacgggccat tttgttttct
     5881 ttcgtttaat atatcttcta caaaatcacg ccgaatacct tttatcgctg tcagtccgaa
     5941 tctgatttct tccttattga ccaactcaaa actggaatta ctttgattaa tggacggcga
     6001 ggaaagtttg agtttatttt tacgtgcttc tgcaatatat tctttcaatt ttgatgtgtt
     6061 atgccgaaca gaattcatca ggctggtaaa gaaagcacct ggatgatggg cttttagata
     6121 cgccatttgg aatcccacga atgaataagc aaatgcatgt gaccgattga atccatagtt
     6181 tgcaaatttt tcgatatatt catatacggt cgctgctgtt ttttccgaat atccctgttg
     6241 taatgctcct gatacaaaat gcgacttttc ttcatctaat acgtcttttt tctttttact
     6301 cactgcccgt cgcagaatat ctgactgccc taatgtaaat ccagccatag tcgaggcgat
     6361 ctgcatgatt tgttcctgat agacgatcac tccatatgtg tttttcaaaa tcgggatcag
     6421 tgaatcatct gggtagcgaa tggcttcttt tcctttcttc ctggcaatga aggtatctat
     6481 attttgcata gggcctggtc gatacaatgc attgactgcg gcaatatctt cgatgttttc
     6541 tggatctaat cttcgtaaaa cgttccggat accagcagat tcaaattgaa atactcctga
     6601 ggtttcgcct cgttgaaaca agagcagcgt ttttgcatca tttagcggga tctcttttaa
     6661 tcggatctca tacccttcta ctcgcttaac agctttcaaa gtaaaatcaa taatcgataa
     6721 gttgcgtagt cctaaaaagt ccattttcaa aagaccgatt gcttctacct cattcatcgt
     6781 atactgggtc agaaaagctt catcagaacc gcgttggagc ggaactagtt caagtagatt
     6841 cttatcgctg ataactacac ctgctgcgtg agtcgaaaca tgtcttggca atccttctaa
     6901 aatcactgca gttttgaata aaagttggtt tttttcgctt gcctgaacta attctactaa
     6961 ttgatgcgaa gttttataag cttgggtcaa tgtgattttc aattgattgg gtattgcttt
     7021 tgaccagcgg tttgcttcac tttgcgataa accaaacact ctagctgcat ctctaagaac
     7081 catttttgct gccattgtgc caaatgttgc gatttgtgcc atatgatatt gtccatactt
     7141 ttctcgaaca taattcaata cttcttcacg tcgattgtca ggaatatcca aatcgatatc
     7201 tggcatcgta aagcgttcgg ggtttaaaaa gcgttcgaac agcaaatcat attctaatgg
     7261 atccacatct gtgatttcta acacataggc tactaatgaa cccgcagcag aaccacgacc
     7321 tgcaccggta acgattttgt ttctatgagc aaaagccatt acatcccata cgatcaagaa
     7381 ataatcatcg aatcccattt gatggatgac agataactcg tagtccaaac gttcttggta
     7441 ttcctttgta tagtcagtga ttcggcgttc cacttgcgaa tagcataact ctcttaaata
     7501 ttctccagct tgttttccat taggaactgg ataatgcgga agtaatttct gatggaactg
     7561 gaattcaaat gaaatcgatt cagctaaacg ctgggcgcct attacagctt gggaaagacc
     7621 cgctttttcg aatcgtttga tcatttcttg ttctgaaaga agatagacat ttgctgttag
     7681 tggtaaagat gcagataaat tttctaaagg tatttggctt ccctctttga tatgtttcat
     7741 cacttcaagt gcaaaaagtt cttcttgttg taaactagcg atgggatgta atgcacaaag
     7801 cgaataattt tcttttatca catactgata ccattcaggc aattgatcag gcgtgttttc
     7861 tgtcaagccg atataaaaat ttctagtaaa aacattttca agtaaaaact gccaatgacc
     7921 gaaaaagtta tcctctttat tgaaagcctc agaaacagaa tgatttgccg gaacaacaaa
     7981 cgctagatgg tcggtttctg tcatttctgc aagttccaat ttttcttgga tcatttttcg
     8041 gctggaaagt ttcatcagct gctgataacc tatttcatct tttgcgtacg caagcaagcc
     8101 ataatttttt tcttcataag tataactaag atgtaatcca atgattggct gtactccttc
     8161 ttctacacaa atacgataga attcaggtgc accgtataag acatctttat ccgttatggc
     8221 taatgaagtg tagccttgtt tttttgcttc ttgtacatat tctcgaatac gtatcgtact
     8281 ttgtaaaaga gaataagaag taattgtata aagttgtggg aaaaacatct tgcattccgc
     8341 cttttttttc gttaaaattc actagcgtta aaaggtttga tccttttact ttcattaaga
     8401 tagcaggcgc tgaagttgat tattccactt catttgcttg cttgtaatct gtaggaatga
     8461 gttctttcgc aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga
     8521 aaatctggta aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat
     8581 tctgggctgt cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa
     8641 catatgattt tactttgaca acaatcattt cattcattgc gggtgtcatt atccttttga
     8701 ttggtgcagt tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc
     8761 ttgagatttc tcaagctctt ttttattttc tactcctcac caccattata atctgctgcg
     8821 aagaacttta cgaaagttga ataaagaaag aagaatattt gacagaacaa acaaacttgt
     8881 cacaataaaa acagcactgt aattataagc atgcgcgact gtcgatccta gcatgggacc
     8941 taaaacttgt ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc
     9001 ttgaggcgta atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa
     9061 gcctaaaata aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa
     9121 ataacagcaa aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc
     9181 ccctagtttt cctagtttcg gtgaagaaaa gacagcggaa acaccagcta tcgaaacaat
     9241 caagccgctg acaaaaagga tattttctgt acttccgctc aattcacgaa tatataaggt
     9301 caaaataggg ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc
     9361 gatcaaaata gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt
     9421 tttctcgatt ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat
     9481 caacaaagcc cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc
     9541 gcccattgaa ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc
     9601 ccatccgctc ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata
     9661 tccagacaaa atcccattcc aaaaccgcat gatcaaaagc caatacgcgt ttggtacaaa
     9721 agccaaagat cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg
     9781 gccttttcga tcagctagat ttccccagat tggtgcaacc acggcagatg caaaagcagt
     9841 cacagagatt gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc
     9901 aacataaact ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc
     9961 aatccacgca atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt
    10021 ctcttttatc atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa
    10081 aatcccgcaa aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag
    10141 aacaaatact gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga
    10201 taatgaataa aatatcaacg atccacaaag caacagaaac ttgattgtat tttcctttga
    10261 ttactttcac aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc
    10321 acaatcccat aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt
    10381 tgatgtctgc aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg
    10441 cttgatttgg tacaatggcg atcagtggag agaataaact acttaatgcg aatagcactg
    10501 ccactactac tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga
    10561 cataagttgt tgtattcgat gtaccaaaga tagcccccac agaagtagcg atcgcatcgg
    10621 caaatagtgc tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt
    10681 cttcttttga gaaaatacct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga
    10741 aagtatcaga taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat
    10801 cactgaataa agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt
    10861 ctttaaatga attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac
    10921 ccataaaaat accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat
    10981 tttttacaac taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt
    11041 tgtcaaagtt ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc
    11101 cgccttcaac gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg
    11161 catttttgat tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa
    11221 gactttcggg aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat
    11281 tgattaatcc gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa
    11341 aaacgaccgt aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta
    11401 catttgcgaa caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa
    11461 atactgcttg gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat
    11521 aactcatggc aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt
    11581 ttttctttaa accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat
    11641 ggaaaactat aataataaaa aataagtaaa atagggcaaa tccatcgaac aatttcaaat
    11701 tatatagacg tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga aaataatact
    11761 taacgttcgt gtttagctta tgtttagcta tttatatagc cataactgta gtttttgtta
    11821 ggaatagctg attgctcttg ttaattacct aaaatatgct agactaaaac aaatgctagt
    11881 tgaggaagga aggaccctta ttgaataaaa aattaattgc catcgatctg gacggaacta
    11941 ccctcaatca agattcgatg attacatcta aaacaaaaga gactctaaaa aaagcaatca
    12001 atgccggtca tcatgtcagt attgctacag gacgcccatt ccgaatgagc catcagtttt
    12061 accaacaatt agaattaaca acaccgatgg ttaattttaa cggcgcatta gtacacatcc
    12121 ctaatcagca ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc gcttttgaaa
    12181 tcttatcgca aaaaaagcag ctaaatttag attttatcgc tgctgaaaat cgtgatactt
    12241 tttttattga cagttttgat ttctttgatg aaaaaatttt tgcttcttct cgtccaggag
    12301 agaagaattt gctttctcct aaaaatttga cgacaaatcc tacatctttg cttgtccgaa
    12361 cagacaaacg ttttgcagga gctgtatctg ctgaattaac acgtcaattt ggcagttatg
    12421 tcgatgttcg gacatggggt ggacctactg ccatcttaga gattgtttca aaaggaatcc
    12481 aaaaagcaaa aggcgtgcaa gagattgcta actacctgtc tatcgatcaa aaagacgtca
    12541 ttgcctttgg tgacgagcat aatgatttgg aattgttgga ttatgcaggt tggggagtag
    12601 ctatggctaa cggtactgac caactcaaag gcattgcaaa tgatgtcact cctttgagca
    12661 atcaggaaga cggtttagct gtttatctgg aaaaactact taaactataa tagagaatta
//
LOCUS       DASHRQ010000075        12581 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.079, whole genome shotgun sequence.
ACCESSION   DASHRQ010000075 DASHRQ010000000
VERSION     DASHRQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.079"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..167)
                     /locus_tag="U2M88_002349"
     CDS             complement(<1..167)
                     /locus_tag="U2M88_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEM8374694.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSN"
     gene            complement(164..817)
                     /locus_tag="U2M88_002350"
     CDS             complement(164..817)
                     /locus_tag="U2M88_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8374695.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1105..1625
                     /locus_tag="U2M88_002351"
                     /pseudo
     CDS             1105..1625
                     /locus_tag="U2M88_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1527..2243)
                     /locus_tag="U2M88_002352"
     CDS             complement(1527..2243)
                     /locus_tag="U2M88_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEM8374696.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(2367..2552)
                     /locus_tag="U2M88_002353"
     CDS             complement(2367..2552)
                     /locus_tag="U2M88_002353"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374697.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            2779..4052
                     /locus_tag="U2M88_002354"
                     /pseudo
     CDS             2779..4052
                     /locus_tag="U2M88_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4159..5190)
                     /locus_tag="U2M88_002355"
     CDS             complement(4159..5190)
                     /locus_tag="U2M88_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8374698.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5197..6027)
                     /locus_tag="U2M88_002356"
     CDS             complement(5197..6027)
                     /locus_tag="U2M88_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8374699.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(6024..6839)
                     /locus_tag="U2M88_002357"
     CDS             complement(6024..6839)
                     /locus_tag="U2M88_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEM8374700.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(6836..7660)
                     /locus_tag="U2M88_002358"
     CDS             complement(6836..7660)
                     /locus_tag="U2M88_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEM8374701.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(7650..8750)
                     /locus_tag="U2M88_002359"
     CDS             complement(7650..8750)
                     /locus_tag="U2M88_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEM8374702.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYSLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            8928..9713
                     /locus_tag="U2M88_002360"
     CDS             8928..9713
                     /locus_tag="U2M88_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HEM8374703.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            9746..10129
                     /locus_tag="U2M88_002361"
     CDS             9746..10129
                     /locus_tag="U2M88_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEM8374704.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10205..10336
                     /locus_tag="U2M88_002362"
     CDS             10205..10336
                     /locus_tag="U2M88_002362"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEM8374705.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10305..10541
                     /locus_tag="U2M88_002363"
     CDS             10305..10541
                     /locus_tag="U2M88_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEM8374706.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(10619..11590)
                     /locus_tag="U2M88_002364"
     CDS             complement(10619..11590)
                     /locus_tag="U2M88_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEM8374707.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            12242..>12581
                     /locus_tag="U2M88_002365"
     CDS             12242..>12581
                     /locus_tag="U2M88_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM8374708.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVSQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVR"
BASE COUNT         3775 a         2831 c         2302 g         3673 t
ORIGIN      
        1 atattactag aaatttcatt tgccatatta tgaatctttt cttttctcgt ctccaaatat
       61 gttttaggaa agataaaaaa aattaatgaa tgaactaata taattattat tccaagaaca
      121 gaaaatattt gtatgaacat ttttggaaat atctttaatt ttttcatttt ctctccaatt
      181 tatatcctac atttcttata gtagttatac agtctaattg taattttttt ctaagttctt
      241 ttatatatac atctattact cgatcataag gagtttcttc gctatctttc catacataat
      301 caataatttg cattcttgtt aacacttgtc cattattatc caataaacat tttaatactt
      361 ctagttcttt tgcatttaca tctatttctt caccatttat tttagctgta tagctattaa
      421 aattaaccga tagatcctta tattcaaact tctctaaatg tccataattc ttcttaatta
      481 aagaatctat tctagctttt aagaccggca atgaaaatgg cttttctaca tatccgtcta
      541 ctaaattagt aaatgcatca attttatatt cttcatcact aaatgcagtc aagattagaa
      601 ttggtaaatt gcttttcttt ctaatttctt tcaacacttc taaaccattt ataaaaggta
      661 tctgaatgtc taagataacc aaatttatat cgctattaaa ttttgacaag gcttctcttc
      721 catctttagc ttgaataaca gtatatccaa attctgaaag atattcactt attccctctc
      781 ttatcatatt atcatcttca acagttaata ttttcatata tacctccact taagcctata
      841 cactagatat tatacagcat attatggctc tataatatct agtgttggtg acgccttctg
      901 ttgccaatac tttttctttt ttttaataaa tgcaaacaga caccttgaaa tcctttagaa
      961 aaaaggcttc tataatattt tgtgttgttg gaaaatcgaa ggcgattttc tactaacact
     1021 ttttcttcgt ttcaactcaa aaagagactc acttttggta aaatttagtc gaccaaaaca
     1081 tcaccctacc aaaggagtcg ctctatggat aatcatacta gaaaattact cggattaaca
     1141 gataaacatt tattttttga tgaagaatgg ctgattgaaa aagaatataa aggcgttcaa
     1201 gcgcagttta ttaaagggag attggatgac tcaccttccc attgtgaaca ctgtggatcg
     1261 attcgtatta tccgcaacgg ttcctatacg acacgtactc agatgttgaa agtcaaagaa
     1321 aagttgacta ttttagaatt aaaacgtacc cgttttctat gctatgattg tgggcagacc
     1381 ttttctgcga aaacggactt agttgatgaa catcatcaac taacaaagga actgaagcag
     1441 gcgattctca tagaactgta cgaaaatcaa tcacgtaatc tgaaaatcag aagaagtacc
     1501 gtcggctcaa gcgatattgg aaactcttac tcaaagattc cacgacattg gagccgctca
     1561 aacggcatta tcatcgactg tttaaacgac caattagtca aacagaaatc gtcgatgaac
     1621 cgttgtgcac aatggtgtcc tgaacgtaca gcgacacctt ccatatccag agcagtcgcc
     1681 acatcacggg gatggatgcc atccaaatta aaagcaacca cgccagtatg atgttcagga
     1741 tcttgcggtc cgtaaatggt gataccgccg atcgcttgta atttaggtaa aacgtaggca
     1801 accagttctt gctcataacg atgaatattc tccataccaa tctcattcaa gtaatcgatt
     1861 gctcctgcaa tgttcggagt accagcttca aatttccatg gcagctcttt ccatgaacta
     1921 tcgtaaagat tgacaaaatc tatcatttca ccgccgaatt cgactggctc catttgttct
     1981 aacaattgac gtttgccata taatacaccg attcctgtcg gcccgcacat tttatgcccg
     2041 ctaaaggcga aaaaatctgc atccaacgct tgtacatcaa ctgtcatatg cggtgctgct
     2101 tgggcaccga ctggactatc ctcaactaca acagcctgtt caaccctcag ccgtttcaag
     2161 cacaacaaat aaccttctgg atctggtttg ccgacctgaa gatcatcgat cgtcacaaag
     2221 gaatcaattt tttccttaat catcggatgt gcttcaaaaa aatagtctag ctcccgcagt
     2281 tcagaattga gactactccg attttccagt gctgctgatc cgctgctcaa aatgttcagc
     2341 atccactggt ctcccaaccg cttgacttat cccttcataa aaggacagtt ctgctgccgt
     2401 cccaacacct tgttgatagt ctgcaaaagg aattttatag ccgaactgct ccagtaaata
     2461 ggcttcagta aatagtgatg aatactctcg taaaacatcc attcactgtc aatcatcgtc
     2521 ccatcaaaat caaaaataag tgctttggtc atcttctttc ccccttaccc aaaacaagca
     2581 aaagctgtgt cctgctctct ctacagtatc agcataaaaa ctgccgtctg aaaagtcaaa
     2641 ggtgaaagaa atgtggcgct ataatttcta ggactgatag taaatcattc tatcagtcct
     2701 tttgagcata aaaaaacaaa acatacagaa gctccgttag aattgaatca ccacaacaca
     2761 atcaaaggag cgttctgtat gcctgatctt catcttatca aactattact aggaatcaaa
     2821 gatggaaaca ttcaaatcac tcatgtcgag aagaaaaaaa tacattcaat caacagtatc
     2881 gttgtttctg ccacgctcat taaaacgatt cagccctgtc ctcagtgtaa acaggtgaat
     2941 caacatgggc tgatcgttaa gaacggaaag aaaaaatcgc tggtccttct caataagtgt
     3001 gcgaatcaac ggacttatct tgctttggca aagcaacggt acctctgtcg tggctgtcac
     3061 acctatttta cggcgaatac ctacattgtt gaaaaaaact gttttatcgc caaacaagta
     3121 cattataaaa tattggagga attgactgaa aaacaagcaa tgacgacgat ttctaaacat
     3181 tgcggcgttt cttggtcaac cgtttcgcga acactcgctt ctctgctccc catgacaaac
     3241 gtgaaaataa attggttgcc tcgctgttca ttggtagatg aatttcgttc attgaaaaat
     3301 caagtcggtc cctattcttt ttcttgtatg gatggcgata ctgggaaatt actggatatt
     3361 cttccttcac gtaagaagaa agatctcatt tcttatttta tgcaatttga acgacgtgca
     3421 cgtttaaacg taaaaatact cgtaacagat atgaacgcct cttatgcttt gctgataaaa
     3481 gagtgctttc ctcatgccaa actcgttgtg gatcgctttc acatcgtcaa gcacctcatt
     3541 cgcagtttta aggatatttg actatgagtc atgaaaagct tcgaccgaaa cgatccaata
     3601 caagccaaac actatcgaca agtgaaggct ttatctcgac ttctgatcaa acgacaggac
     3661 acgcttgttt atgataaatg gacaaaatgg cgtaattttg gttgggccta tttaaccgaa
     3721 agtgaagtcg tagaacgtct actttctact tctgatgaac tacgaatagc ttatgcttac
     3781 tatcaagaaa ttcttcaagc tttttatgac aaagaagcgg acatcttctt tcagcttgta
     3841 aaaacaatgc ccaagtcggt tccaagagaa ctgcatcata taaaaaaagc gtttataaac
     3901 tatgaatccg gcattcgttt agccttagag ttgccttact caaatggcaa aatagaaaat
     3961 ctgcataccc acatcaaagc attgaaacga atagcttacg ggttaggatg agaaccagaa
     4021 ttttcttaat caatcatctg atcacttatt aaacataaaa aaacatctaa agcagaaaat
     4081 caattctacc ttagatgttc ttatatcact ctatcagtcc tatttgacaa agagccagaa
     4141 atgtttgccg actatacact agctttgttg tgcaaaatgc tgagtatcaa tttccacagg
     4201 gattgctaga atatctttca gctgaatgta taaggcatcg ctaattttct cttcccgatc
     4261 gttcaaccga tccactaaaa tttctttgcc ctccagcgtt tgaaaaacat accgttgaat
     4321 gttgcctaaa ataaatgatt ccttccatag aaccggaatc ttaaccccct gaggaaccgg
     4381 ttcaaattga atcagctccg gtcttaaata ataaatgcag cgattgccct tagcggaaaa
     4441 ctcgtgggta tactgattca gctcatacca ttcgaaacga ttatagttgc cgataaattc
     4501 cgcaacaaat cggctctgag gattctgata caattccttc gaggtggaaa cctgagccac
     4561 ccgcccagct tccatcacaa aaatccgatc actgattcgc atcgcctctt cttggtcatg
     4621 agtcacaaaa atcatcgtca tccctagcga ttgctggata gcgcgcaaat cccgctgcag
     4681 ctgttttcta attcgggcat ccaaagcact taatggctca tccagcagca ggactttcgg
     4741 ttcagtgacc aatgagcgag ccagtgccac ccgttgtcgt tgaccgccag aaagaaactg
     4801 gacattttta tctgcctgct ccgtcatatc caccaatgat agcatctcaa aaaccttttc
     4861 ctgaatcacc tcaggactca gcttctttac cttcaaacca aaggctacat tttcaaaaac
     4921 agtcatagtt ggaaacaatg catactgttg aaaaacaaag ccgatattgc gatccttcgt
     4981 aggcttatcg gcaatattct cattgtccag cacaatctca ccgctctttg gttgttccaa
     5041 tcccgtgata cagcgcagca acgtagactt tccgcacccg ctgggaccta gtaaagtaac
     5101 cagctcaccc ttttcaatct gaatttcatc aatagctaaa atctgttttt catcaaaaaa
     5161 cagattgaga tcatgtattt ctaaaaacat tttccgtcac aactccttta agggcttctt
     5221 tttcgactgc ttctcttccc tcgtctgctt aagggacagc tgttgctttt gtcgataagt
     5281 cagcataaag gtagttaaag caattcccac cataaacagc aaatacaaaa ccatcacagc
     5341 gctagccaaa tggccgtttt cgttcatccg ccgcagcata tagatgcgta ccgtctgaaa
     5401 tcgaccgcca atcaacaggt tcgtcagcat gtactcccca aaaaccgaag aaaaaatcat
     5461 cagagaaact agggtcacac cgatacggat gtttggaaac agcacctgga taataatcgt
     5521 agacatcggg gcgcccaagg tttctgctgc atccactaat tccttcagat taattaagcg
     5581 catctggttg ttaagactaa gatagacaat cggcaacgcc gtaataaaca atgcacctac
     5641 caacactaca aacattggga taccagtttc agcatacgtt cgaagcaaag ccgttaccaa
     5701 aatcactcct ggtaaagcat acggcagcag gagcagcttt tccagccaac gattcaaacg
     5761 agggaaatac aaatgaatcc aaataatggt gggaatcatc aacaacagaa tcgtcaccaa
     5821 gaccacacct gctaataaaa gtgagcgacc caccgcagca ataaattccc tgtccgtcag
     5881 cagctgttgg aaccattgca aggtaaaatc actaggtaaa agcgattttg accatttagt
     5941 ggagctggca tacaaaatcg ttccaataat cggcagcaag taatacagga caaagcagaa
     6001 caaaatcggt tttattaact ttttcacgtc tgcaccacct tgcctttctt ttgaataatc
     6061 aacattgccg ttaccagcag gacacctaaa gcgactgcca acgcactgcc taaattaggc
     6121 ttggcgaaca catctcccga cactaaagcc gcaattcgaa tcgttaaaag atttacatta
     6181 ctgctggtca gggcatacgc tgtttcatag gtccccatgc cattagcaaa taagatgaca
     6241 aaggtgctgg caatcgatgg tcgcaaaaaa ggcaaagcca ccttgaacca agcatacgct
     6301 gatgaagcgc ccagcaattg tgcggactcc agccattcct ttttcacttc cttcatgctg
     6361 ggatacaaaa acaagacccc taacggaatt tggaaatagg tatacgtgat ggtcaatccc
     6421 caccaagaat acaaattgaa gtccgctaga aatggcaaaa tcattttcag cacacctaca
     6481 ttccccaaca aaataataaa agagaatgcc agcggaatcc cagcaaaatt agctgccaaa
     6541 ttggaaacaa aggtgatttt ctcctgcaat ttagtcgata gattcattaa gcataaacat
     6601 aaaataaacg tcccgattaa cccaatcaca ctggagctga cagcaatcag tacgctgtta
     6661 acaaaggcct gtgtgtaata tttgttgctg aaaatatcac tgtaattggc caatgtccac
     6721 tggttagtta aatcactgtg caagctgcac agaaccataa aaagcagcgg cagcagtaaa
     6781 aatgcacaga agataaagga ggcaggaacc agaaccaagc gttttttcat ctctttcatc
     6841 ccctcatcaa ttcttgaatg tcctgcattt tctctgacgg tgcaattcct aataaccagc
     6901 aaaacaatgg agccgccagc agctgtggta cagtttgttc cttcagcttt tggcgtttat
     6961 ttgaaatcaa ataaaaagga acttcacgat gtgcagccaa gctgccgcca tgcagtccgt
     7021 gcgcatccat cccgtgatcc gcagtcacca cgacttgata acctttttgc agccattcag
     7081 gaaggtaaat agccaataat ctgtccgcct gattaaccgc tgctgcatat tcatgagaat
     7141 cggcggtaaa tttgtgaccg acatcatcaa tattcatcga atgtacgacc aaaaagtctg
     7201 gctgatactg attcactaaa taatgggcat ccgcaaataa atgcgaatca gggtaagtgt
     7261 cctcactata aaagatgcca tgctgaatca gtcgctcctc ctgatgctgg atgcgatcaa
     7321 ccaaacgatt atagggggct tgattgtaca actcgctgta ccagtggtaa gcggcggctc
     7381 ctgtttttcc gccagcttcc ttaactaatt caaacagcga ccactctttg gatcgctgag
     7441 catggtaatt actatagatg ccattttcat aggctggaac gccggtcatc agtacttcgt
     7501 ataacggacg tgaattgctg ggcagctcac tttttacctt caataagctt gcttgctggt
     7561 gctcaactaa atgattcatg aacccaagct gggcgacggc tgtgtcgtag cgacagccgt
     7621 ctagtataac tacaatcagt ttgtcaagtc tactttgcat aggtcagtac ctccgcctgc
     7681 caaagctctg gaagctctac agaggtcttg ttccaagctt cattgtcttc aactggcacc
     7741 gccgcttcat aagcatcctc cggcagtaat ttttcagcta cttctttagg cagctctacc
     7801 ttgcgaatag gtcgcgcata gccctttgcc aaattgattt gcccttcatc agataaaata
     7861 tattcccgtg ctagctttgc cgcattggga tgaggggcat ttttattgat taaggttgta
     7921 tacccgctaa tgatggagcc gtctgttggc acagttacct caaaacgctc ggagtcaatt
     7981 tggtcacgat aatttagtcc attgaagtcc caaacaactg ccacatcgat ttcacctttt
     8041 tcaagatttg ctacgctggc atcaacggtg gacaaacggc ctttttctgc catttttttg
     8101 tagtaattca gacctggctg aatatccttt tcgtcacctc cgttggcaat cgccgcagcc
     8161 aatacagcaa attgtgcttg gttggcaatc gtcacatccc ccacgctaac ttgatagtca
     8221 ccctcttgca gctccttcca gctagtcggt gcttgcttca cattctcttt atcggtgata
     8281 aaggaaatgg ttcccgtata gcttaacaac caatccccat catcatcctt tgcccaatcc
     8341 ggaatttcct cccaatagtt ggtcttataa ggtaaggtta actctttgct ttcagccaac
     8401 ggaccgaagc tgataccgac atcaccaata tcggcggttc cattttttcc ttctgattca
     8461 aacttagcca attcttctgc actggacata tccgtatcca cgtgacttaa gctgtattct
     8521 ttttcaaggt cgttccaagt gcctacccaa tttgcccaag tatccggcat tcccaagctg
     8581 gcaatctctc cttcttcctt ggctttttct ataatcgtat ctaaagagtc gtctgccgca
     8641 gccgattgct tcgttgcttc acctgagacg cagcctgtca gcaggatact tactgttgct
     8701 gcacaaaata aaccgatagt catgttacga attttttcat tcattttcat tcattcttct
     8761 cccctttcga taattccatt ctaacaaagc tttgtaatga gagaatcagt ttcttgaaaa
     8821 ggtttcatga acaatgtaaa ggttgtatat ggtttgtaaa tttcgacgaa ttcccgttaa
     8881 ccttctaaag cttttgcggt atgatagtag aaaaaaggag gctccctatg cagtattatg
     8941 atcagcatct ccacacgtat ttttcctttg attcagagga aaaatttgaa aactatttag
     9001 cctatcagcc ggagtttttc gtttctacgg atcactttga tttaaacaat ccatctaccg
     9061 gaaaagatga cattccggat tatccagcct atataaacaa actggactct ttaaaagaaa
     9121 cctattccac caaatttctg cgaggtatcg agattggcgt tgttccagga caagaagagc
     9181 ggatcgtgga ttacctcgct cagcatcctt atgatttaaa gctggtaagt attcatcaaa
     9241 atggccgctt tgattatatg gacgatgtgg ttttaaccaa agatcccatt caaactacca
     9301 aggaatattt tgatcagatg ttgcaggtct tgacggattt tccagaagga gatattttga
     9361 ctcactttga ttatggatta cgccgcttca ccttcacagc ggaagaattc gcggagcatt
     9421 ttgaggctca gctgctaacg attttcaaaa aggttgttga acgagaattg gcgatggagt
     9481 taaatgccaa gagcttcttg aaatacggca acgaagacct ttaccgttat gccgttcctt
     9541 tgtatcaaag tgtcggcgga aagctcttta ccttaggctc ggatgctcat gttgccgaag
     9601 attatcagct gggctttaaa aaaatgagtc agctgttggc agactgtcag gtagaaaagt
     9661 tgctagtcat tcaaggaaaa gaacgccgcc tgacgccgct gccgattttg tgattcctat
     9721 ataatagaag aaagtggtga aaaaaatgag caacctgtgg gactacaatc aagaggcacc
     9781 catccattat ttaattgcgc gacattggga tgcgttgaaa attgaggcgg tttgccgatc
     9841 cttgttagca gccgttccaa aacaacaact ggaaaatttt ttggtggctg acagcttgca
     9901 gcgggaaaaa gttcaggctt attttgcagc ctttaaagat cagcctttgg aatatttgca
     9961 tgcgcagttt catctttttt atcaagtagc cgccccagat gactacaacg atttacgagg
    10021 acagctgcaa ttgacctttc aagcggatga aaccgcctat accgttttat tgggaatggc
    10081 tcgccttggt gatcaggcga aggtcgagtg gcggattttc gatatttgat ggcaggatca
    10141 aggcgtttca gttgcaaacc ttcctctctt tcaattagaa aaaccgcagc aggaagaagt
    10201 ttgtgtgctg gtcaccgatg ctggtgaaat tcgtctccgc ttatttcctg accaagctcc
    10261 cctggctgta gccaactggc gctccctagc tgagcaaggc gtttatgaca acacaccttt
    10321 cggctcgtgt cattaaggaa tttgttattc aaggtggcgc tttggatggt tctggtgatg
    10381 aagctgtttc cagctatgag ggctttttcg ctgatgaagt gcatcgcgga ctttatcatt
    10441 ttaatggcgc cttggcctta ggggaccacg gcccccacac caacggcaac cagtttttta
    10501 ttgtgcaaaa cacaaaggcg caggctgacc tgctaatgta aagcaagcat acttggagct
    10561 tggaggacta ccggagctgg atggtcgtta tactgttttt ggacaggtat accaagggtt
    10621 agaaatcgta gcagcgatcg cagctcagcc gacaaacagt gaagatgtac cgattgagcc
    10681 agtgttcatt cgacaaatta acttcgaaaa aacatcgtaa aaagacactc actgacttca
    10741 gcaaaagctc tattcttgag ctggctgcag ctagtgagtg tctttcattt atccataaga
    10801 taaagcttgg attcaataaa taaataaatg ttttggttaa agctgtgacc agcaaatgag
    10861 gaaccgtgcg agccagttga tctgccaatg caaacatttc tttaggtgaa ggcgctggcg
    10921 tgtctgccaa cggatactcc agtcccaatg cttggtaacc gtgcatacca tagcagtggt
    10981 acggtagtaa ttctaacggt acgaaccgat caagatctgc cacaaaacaa gcaatcgtca
    11041 gcagctcggt caggttcata ttgatacctg gaaccacagg aactcgaatt cgcacttcgc
    11101 agttaggata ctcaattaaa aagcgacgaa tattcttcaa aatttgttgg ttatctaagc
    11161 cggtccattg tttgtggagc ttgctggtaa acatttttac atccacaaac aatgtatcca
    11221 catagggagc taccttttgg atttcattat aagcaccaca aaagctggtt tccagtacgg
    11281 tattgattcc aatgtatttc gatttttgca aaatttcttt agcaaaatcc gcctgaacca
    11341 gtgcctcgcc tccgctaatg gtcactccgc catccgagtg aaagtaaaaa acagcatcct
    11401 tctcaatctc atccattacc tcttcagcgg tcatggtctg gccaaatcct ttttcaatct
    11461 gcttgctttg tgattcggga atcgaacacc aggcacagcg taatgggcag ccctttaaat
    11521 aaacaacggt ccgtaaacca gcaccatcat gtagagaacc ctgttctatt cttaatactg
    11581 ttccttccat atagattttt gaattacaca ctagtttaaa ctaaaacaca caaggaaaaa
    11641 cttaccattt aatcataaaa ttagctcata taaatatact aggatgtaaa atagactggt
    11701 caaaatctgg tcaaaaaaga ggactaatac aggtagtcct cttttttgtt attttaatat
    11761 tctaaatcat tttttattgg tttattccct tattcgatgt agactcaaat cactatatca
    11821 ctagatatca aacggcgtca tacggaatca aaaacatcaa atttgacctg ttaaatggtc
    11881 attgaataac attcaaaatc aataaaacgg agtcagacac ggagtcaaaa acgctgagag
    11941 taaaaaatat atacagcctt gtaggtaaag taccacaggg ctgtatattt tttatttcag
    12001 attaccttaa atgattccat ttacttttta tctcattttt tcctactagt tagtatcaaa
    12061 tactctccta ttttctcaat aaactatttt tggatacatg gtatctgaaa cagctaaaac
    12121 cttgtcatta cagcaaaaaa tcacgttatc cagactgtag acaaagtcca ttttatggat
    12181 gatgtctaca gtcttttctt ttataataga tttatgaaaa cgaatgcaag gagtgatagc
    12241 tatgatgtct aaaaacaata cgaatggtag aaatcaattt gcgatgctta cgattgatga
    12301 tctagtttct caagatcatc tcgttcgaaa gattgatgcc gcgcttgatt ttgaatttat
    12361 ttatccaatc gtcgaagcca cttactccga tttaggtcgc ccaagtatcg atccagtgat
    12421 tctgatcaaa ttggtgttta ttcagtattt attcggtatc cgttccatgc gacaaaccat
    12481 taaggaagtc gataccaatg tcgcttaccg ctggttttta ggctactcct tcgaggagaa
    12541 aatcccccat ttctcaactt ttggaaagaa ttatgttcgc c
//
LOCUS       DASHRQ010000076        12573 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.080, whole genome shotgun sequence.
ACCESSION   DASHRQ010000076 DASHRQ010000000
VERSION     DASHRQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.080"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1014
                     /locus_tag="U2M88_002366"
     CDS             <1..1014
                     /locus_tag="U2M88_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEM8374709.1"
                     /translation="ELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQGTFI
                     DYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLNDTW
                     LVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDP
                     FTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQLVS
                     AHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKGDINA
                     QILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQ
                     ERE"
     gene            1025..3229
                     /locus_tag="U2M88_002367"
     CDS             1025..3229
                     /locus_tag="U2M88_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEM8374710.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            3245..3394
                     /locus_tag="U2M88_002368"
     CDS             3245..3394
                     /locus_tag="U2M88_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374711.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            3470..4063
                     /locus_tag="U2M88_002369"
     CDS             3470..4063
                     /locus_tag="U2M88_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEM8374712.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            4076..4975
                     /locus_tag="U2M88_002370"
     CDS             4076..4975
                     /locus_tag="U2M88_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEM8374713.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            4978..5451
                     /gene="ssb"
                     /locus_tag="U2M88_002371"
     CDS             4978..5451
                     /gene="ssb"
                     /locus_tag="U2M88_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEM8374714.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSTTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            5817..6077
                     /locus_tag="U2M88_002372"
     CDS             5817..6077
                     /locus_tag="U2M88_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374715.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
     gene            6522..6728
                     /locus_tag="U2M88_002373"
     CDS             6522..6728
                     /locus_tag="U2M88_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEM8374716.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            6728..6979
                     /locus_tag="U2M88_002374"
     CDS             6728..6979
                     /locus_tag="U2M88_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374717.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTTETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTELEN"
     gene            6995..7411
                     /locus_tag="U2M88_002375"
     CDS             6995..7411
                     /locus_tag="U2M88_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374718.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGACYIMK"
     gene            7393..7665
                     /locus_tag="U2M88_002376"
     CDS             7393..7665
                     /locus_tag="U2M88_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374719.1"
                     /translation="MLHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTV
                     SHNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVENFMD"
     gene            7832..8233
                     /gene="tnpA"
                     /locus_tag="U2M88_002377"
     CDS             7832..8233
                     /gene="tnpA"
                     /locus_tag="U2M88_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEM8374720.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTEEQIRKYIQNQKKK"
     gene            8245..9390
                     /locus_tag="U2M88_002378"
     CDS             8245..9390
                     /locus_tag="U2M88_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM8374721.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKLSTEIIKNHDII
                     CIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVDKWFPSSQICSN
                     CGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA"
     gene            9628..9891
                     /locus_tag="U2M88_002379"
     CDS             9628..9891
                     /locus_tag="U2M88_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HEM8374722.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            9885..10235
                     /locus_tag="U2M88_002380"
     CDS             9885..10235
                     /locus_tag="U2M88_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEM8374723.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(10259..10873)
                     /locus_tag="U2M88_002381"
     CDS             complement(10259..10873)
                     /locus_tag="U2M88_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM8374724.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            11323..>12573
                     /locus_tag="U2M88_002382"
     CDS             11323..>12573
                     /locus_tag="U2M88_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEM8374725.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLE"
BASE COUNT         4586 a         2063 c         2488 g         3436 t
ORIGIN      
        1 gaactaaagc caaaacatga acggcaaaat ttcttagttc gagacgatcg tttagatcat
       61 gcggtcgcct ttttatggaa agatccacag acaaatgaga cagtaggggc gagttatcaa
      121 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
      181 aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca gttaaaattt
      241 tttgagagta gtattgattt acttagctat gctgcactaa atcgtgatca gttaaacgat
      301 acgtggttag tttcgatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
      361 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
      421 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agttgatcca
      481 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaaa
      541 gaatacaaag tcatttatga agaagtcgca aaagaagaaa agatcacacc agaagctatt
      601 atggccatac acaagacaga aaacaatctg caattaacga accaattggt ttccgctcac
      661 aaagtaaatg cgtcttttgg tcagcagctt tctgttaacg attcaataga agctattaat
      721 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag cttgtgaacg cgtggatggt
      781 acgtacgatt ttgatcgttt ttatcaagaa aaaggggata ttaatgctca gattcttttc
      841 tcttataaag cagaacaata ctacaaaggc tataaaaatc atgaacatga atttgtacct
      901 gaagtaaaaa aggattggaa tgatcaattg aagcatgaaa tctaccaaca agagatcaga
      961 aagcaaaaac gtgccatgtt gtttcaacaa ggtagacagc aagaaaggga gtgaaatcgt
     1021 catgatgaaa accgttattt tagcagaaaa accgtcacaa gcgaaggctt atgccgatag
     1081 cttttctaaa gctacccgaa aagatgggta ttttgagatc caagatcgat tgttttcagg
     1141 agaaactgtg atcacgtatg gttttggtca tttagtcgag ttagattcgc ccgatatgta
     1201 cgatgaaaat tggaaacaat ggtcattaga gcatttgcct attttcccta cacattatca
     1261 ctatcatgtt cctaaagata agaaaaagca attcaaggtg gtgaaacagc aacttcaatc
     1321 ggccgacacc attattattg cgacagatag cgatcgtgaa ggggaactga tagcttggac
     1381 gatcattcaa caagcaggcg ccgatcatgg aaagatattt aaaagacttt ggatcaactc
     1441 cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagatgcag aagagaccta
     1501 cccaaaattt gaagaagcac aagcacgcca gatcgccgat tggttgatcg gcatgaacgg
     1561 tagcccgctg tatagtttac tcttgcagca aaaaggcata ccaggaagtt tttccttagg
     1621 cagagtccaa accccaacgc tttacatgat ttatcaatta caggaaaaga ttagaaactt
     1681 ccaaaaagaa ccatattttg aaggaaaagc ccaagtaatc gctcaaaatg gcgcatttga
     1741 tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag attatttaaa
     1801 agaaaagggg gttcaattgg gaaaacagcc aggtacgatc caccaagttg aaaccgagaa
     1861 aaaaagtgcg gcaagtccac gtcttttttc tctatccagc cttcaatcta aaatgaatca
     1921 gctcatgaaa gctagtgcca aagatacttt ggaagccatg caagggttgt atgaagggaa
     1981 atatctaagc tacccacgaa cagatacccc ttatattaca gaaggggagt atgcgtatct
     2041 gttggatcac ttagacgaat acaaacactt tttaaaggcc gaagccattc ctactcctat
     2101 tcatacaccg aatagtcgat atgtgaataa taagaaagtc caggaacatt acgcgatcat
     2161 tcctacaaag acggtcatga cggcagcggc ttttgaacaa ttaagtccac tgcagcaggc
     2221 tatttatgaa caagtgttaa aaacaacggt ggccatgttt gcggaaaagt atacctatga
     2281 agaaacaacg atcttgacgc aagttcagca gcttcagcta aagacagtcg gaaaagtacc
     2341 agtagatcta ggttggaaaa agctgtttgg caaagagagc gacagaaaag aaaaggaaga
     2401 agaaccgcta cttcctaaag tgacaaaagg agaagcggtg acggtagatc tacaagtcct
     2461 tgaaaaagaa acgaagccac cacaacctta taccgaaggt acgttgatca cagcgatgaa
     2521 aacagctgga aaaaccgtgg atagcgaaga agcacaatcc atattgaaag aagtagaagg
     2581 aatcggtaca gaagcaacac gagcaaatat tattgaaact ttgaaacaaa aggaatatat
     2641 caaggtagaa aaaaataagc ttgtggtgac aaataaaggg attttacttt gccaagccgt
     2701 ggaaaaagag ccgctgctaa caagtgccga aatgaccgcc aaatgggaaa gttacttatt
     2761 aaagattggt gagcagaaag gaacacaagc tactttctta gctaatatcc aaaaatttgt
     2821 ctctcacttg ttggaagtcg taccgggaca aatccagtca acagattttg gatcaacgtt
     2881 acaagaggtg aaagcagcca gtgaaaaaca ggaagcaacg cgtcatttag gtgtttgtcc
     2941 aaaatgtcgg gaacaagaag tcttgcttta tcaaaatgtg gcggcatgta cgtctgaagc
     3001 ctgtgatttt aaattatgga caacgattgc caaaaagaaa ctcacagcca cacaactgaa
     3061 agagatcatt caaaacggcc gaacaagcca atcagtcaga ggactaaaag gtcagaaagg
     3121 ttcgtttgaa gcaacgatcg ttttaaaaga agactttaca acaggctttg aattttctga
     3181 aaagaaaaaa acgaactata agaaaagaac acgccgcact acaaaataat gaaggggtta
     3241 tcctatgcga aatacaacac acaaacaaca agaaatcgaa aatcaaaaag caaaaattcg
     3301 tttagaacaa gagaaattac gtgcaaataa aagaagactg caacaattaa agaaccagca
     3361 gcgcattcaa aaagaaatcg aattagatcg atagacgtta atcttgttac aaataaacta
     3421 ctatgatata tttgatgtag tgtatttgta acgaagggag gggaagtaaa tgcaagataa
     3481 actgaaagaa ttgttggcta ttcgcgatgg aaatttatct atgaaggaag ccaggaaatt
     3541 tggaatcgca gcaaccactg tacaaagatt ggtgaatcaa ggaaaattga tcaaagttga
     3601 tcgtggttat tatgtagagg atggtcatgg aatagatgat ttattttggc ttcagcaacg
     3661 attttccagg ggaatcttct ctcatgaaac gactttagat atttaccagc tttctacgaa
     3721 tatgccgaaa ttcattcact taacttttcc ccatggctat aacgttgatc gttcaattac
     3781 aaaagagcag cagttacaat ttcatttcgt gaagaaagaa gtttatgaac taggaaaagt
     3841 agagggaact tcctttcaag gaaatcctat tactatgtat gataaagagc gtacactttg
     3901 tgatatttgg aatccgagat atgaatttga atatgaaatg aaattagaag cattaaaaga
     3961 gtacatggaa gacccattga gagatccaag taaattaaga gattacatga caaaactaca
     4021 tgtagaaaaa gaaatgaaat accacatgca aagcttatat tgatggagga aaaagatgac
     4081 accgacacaa tttgcagcaa aaacaagaaa tttagcaaag agtacaggat taaatgcaca
     4141 gttagtgcaa cggcatttta tgatggatcg tctaattgaa caaatttctg aatcaaagta
     4201 caaaaatgac ttcattttga aaggcggctt tttattagga tcaaaatatg gaatcgatag
     4261 aaggacaaca attgatatcg atacgacatt tagaaatagc aaattaactg aagaaaaatt
     4321 gaagagcatt tttaatgatt taactagaaa acccacaaaa gaaggcattg ttttctctat
     4381 tcaaggaatg aaggaaacaa gagaagccga tttttatcct ggatttcaaa ttaaattggt
     4441 ggctaattta gagaaagtac gtgtaccgtt taaaatggac gtgacaacag gagattctat
     4501 ttatcctgaa gtagatacac actatcacca attaatgttt gaagataaaa aagtggagat
     4561 tccagcttat ccaacagaac aaattattgc agaaaagcta agtgctacgt tcagctttgg
     4621 cacagataat tcgagagcaa aagattacta cgacttattt accattccga aactagaaaa
     4681 tatcgatacg aaagagttat acaaaagtgt tcgaaataca tttacaaaac gtggggatac
     4741 aaaaccgttg tccttttatt atgaaagaga aatggataag atcagaggaa atgactattt
     4801 ggaaaagcag tgggaaaaat atcgctcagt taacacgttt gcgaattcca tttcatttac
     4861 agatacagtc aacgaaattg atcacctaat gaaaagtgtg atcaaaattg aaaatcaaga
     4921 aagaaaacgg cagtttaaaa gatcacaaca aatgaatcaa gaaatggaga gataaagatg
     4981 attaataatg tgacattagt aggacgctta accaaagatc ccgatttacg ttatactgca
     5041 agtggcacag cagtagcgac ttttacccta gccgtcaacc gaaatttcgc gaatcaaaac
     5101 ggcaatcgag aagccgattt tattaactgc gtgatttggc gcaaaccagc agaaacaatg
     5161 gcgacattag ctaaaaaagg cggcttgatc ggtgttgtcg gacggattca aacacgaact
     5221 tatgacaacc agcaaggaca acgtgtgtat gtgactgaag ttgtagcaga taactttcaa
     5281 ctattagaaa gtaaagcagc gacagagagc cgtgcgcacg ctgatcaatc aaccaccagt
     5341 ccaagcccca ctacctttga acaacgggat acaaacaatg gattggacgc ttcacaaaat
     5401 ccatttggtg gacaatcaat cgatattagc gatgatgatc taccattcta agtagcattt
     5461 tctattggtt gcatttgcta cggtttatgt tataatttag tcaacaaaaa gaattttcaa
     5521 tctataacca aaaaccacta gctggcgggc tagtggtttt cttttacgtt tctattataa
     5581 gaattaacgg gtaggcttac tgtctttctt ggcaaattta caatcaagcc atttgcagat
     5641 atagtgcgcg gctacttctg ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc
     5701 tataacctcc tccccttgcc agattaggcc ggggtagcag acagcaagac tattatagca
     5761 taattgaaat attcaaatga ttggattttt gctataattt tagcgaggtg attgtgatga
     5821 acaaatatgt tggtttgctt gataaaatcc gagtaataaa aacgcaaccg ttattggttc
     5881 gctttacttt acaaacgatt catgaatcga ttaattgtgt agttgcagat attgaaatta
     5941 tcgataaatt gctaattatg gatgatggaa aatacaatat tgccgtcaca ggtcacttca
     6001 ataaacgaaa ccagctagtt attgctagca tgtatgttag aaatcccgat cagtttacgc
     6061 gattgatggg catataaaaa aagaagagtt gcaatcctaa tgtaggactg cagctctttt
     6121 tgttttacat agaaacaaga aaaatgaaaa ggccttttta ggaaggcgcg agcgccttct
     6181 caattgaaaa acctctctca ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac
     6241 gttgcacgtt cactaagata aagggtcttg caaagcaaga caaaatagaa aaaacttcct
     6301 ctcaccaaaa ggttcgttcc tgttcgttat ctctttattg atttgccaga ccggcaacaa
     6361 gttgcctttt atcaccaaaa agcgacaagg acaagggtaa atggcacgct gcgcgccctt
     6421 gtccttgtcg cttttcgttg attcattctg gccaacaaat caataaagaa agaactaacg
     6481 aaaggaagta aaacctttcc taagaggagg cagaatcaat catgcgaaag aaatttgatt
     6541 attggggtgt tcccttcagc gaattattcc caaactatca tgcaccacat actgtcgaat
     6601 gcgattgtgg agaaagagcg aaatgtatca aaagctatcg tttgtatcaa tgtccaacat
     6661 gcgggaaaaa atataccttg tcttatggcg attatatttt gattgatgag aaaggaaaga
     6721 aacgttgatg aaaacacaac acaaattatt gactgtaaaa gaagtcgttc aaattttggt
     6781 ggacaaagaa gtcatttctg aaaaagtagc agaagcttta cttattccgc ggtactggac
     6841 gacagaaacc cctttagtag aggatgcacc gactatgcta tttgaagacc tagtgaagat
     6901 tgcaggatat gaactgcatt atcaagaaaa cttggaaact tatgaaaacg aatattggtt
     6961 aaccgaatta gagaactaaa aaggagaaaa aaatatggct caaaattact atgacgaatt
     7021 tgtcaaatta cctttggata aaatggcaca aaaaatggag gatatgacat tcctatacaa
     7081 tgagacacgt gttccaaaaa aacattataa ggaaagattg tcggtagctg tggaagaaat
     7141 gattgaatca ggtgtcgaaa tgaatctcat tgcgacgtat tatcgaacac tggaagaact
     7201 gaaaaagcaa aatgcaaaat ggtttttcca agccttacta tgcttggaag taggcgtcaa
     7261 accaagcaca atcaaacctt ctgaatacca agctttagaa ctaacgtatg cgaaatttat
     7321 cgaaacgaaa aaagctaaga ctgtttcaag tgaatggtta gattattttg aaaatatcaa
     7381 taagtatggc gcatgttaca tcatgaagta atcagacgga ttcaaaaaga ctttagttgt
     7441 atggaagaaa tgaagtactt tgttcaaaat gttgaagaac agttatattt ggaaagcgaa
     7501 acgagtcaaa caaaggtcac agtcagccat aatcgtttag atgtgtataa acgtcgtcct
     7561 gaatcattaa aaaaaggagc ggagcttcta cgactagaaa cagaaaaata cgtcatgaat
     7621 gtttacctag aaaaaagtga gttggtcgaa aattttatgg actagccgag aacacccgtg
     7681 attttaatca tgggatgaat cggattcagt tgtgacctag ctttagctag gttaaacaac
     7741 tgacaaatat aaactaaata atagtgaaat gtcttgcaat aattgatttt gtagaaatac
     7801 atatattaaa ataattgcga ggtgtttttg tatgtcagaa attcattatg gacggggtta
     7861 tgtctattct attcaatatc atcttgtttg gtgtgtaaaa tatcgacatg atgttttgta
     7921 tggtcaggta gatattgatg tcaaacagtt tttgaaacaa attgctgatg ataataatat
     7981 aaaaattata gaaatagaat cagataaaga ccatattcat ttactcattg aatgcacccc
     8041 acaacactat attcctagta ttgttaaagt atttaaaggc gcatccgcta gactgctgtt
     8101 taaaaaacat cctgaactta aacaactcct ttggggtgga catctttgga atccaagcta
     8161 ctttgtagct actgttagcg aaaatacaga agaacaaatt agaaaatata tacaaaatca
     8221 gaaaaagaaa tgaggtgaat ccgtatgcta caacataggg cttataaatt tagaatctat
     8281 cctaatcaag aacaagaaat attaatgact aaaactattg gctgttcaag atttgtctat
     8341 aattattttt tgaatttgtg gaatcatgaa tatacgacta ctggaaaagg attatcttat
     8401 aattcttgtt ctgccatgct tccacaaatg aaaagaaatg cagagacaat ttggttaaaa
     8461 gaagttgaca gtattgcgat tcaatcctca ttgaaaaatc tttctgatgc tttttctcgc
     8521 ttctttaaaa agcaaaataa acgaccacaa tttaaaagta aaaaacatcc tatacaaagt
     8581 tatacaacta aaaatgtgaa taatagtatc gaaattagaa acaatttcgt aaaactacct
     8641 aagttaggat ttgttaaatt tgcgaaaagt caagagccca aagggcgaat cttaaatgct
     8701 acgattcgaa aaaattcgag tggtaaattt tttgtttcca tcctgtgtga agaagaaata
     8761 gttgagtttc ctaaatctaa ttccgctatt ggaatcgacc ttggtataac tgattttgca
     8821 atcctttctg atggtcaaaa gattgataac aataaattca cgtcaaaaat ggcaaaaaaa
     8881 ctcaaacgtg aacaacgtaa gttgtcaaaa cgtgcattac tagctaaaaa gaaaggcgtc
     8941 aatctctttg aagctaaaaa ttatcaaaaa caaaaacgaa aagtagcacg actacatgaa
     9001 aaagtaatga atcaacgcaa tgacttttta aacaagttga gcacagagat tatcaaaaac
     9061 cacgatatta tctgtataga agacttaaat acaaaaggta tgttgcgtaa tcataaacta
     9121 gcaaaaagca tttctgatgt gtcttggtct agttttgtga ctaagttaca atacaaagct
     9181 aactggtacg gtcggaaaat catcaaagta gataaatggt ttccatctag tcaaatctgt
     9241 tcaaattgtg gacacaaaga cggcaagaaa tctctagaaa ttcgagaatg gacttgtcct
     9301 gtttgtcatg ctcatcatga ccgagatatc aatgctagta tcaatatttt gaccgaaggt
     9361 ctaagattac atgtcatggg attagcttag atccaatcat caaaaaccgt aggaactacg
     9421 gggatagctt ggtaaataag agaaaccgct gtgagtaaag aaatacgctt gcaagtatgc
     9481 tctattccca agaatctcgt gactttagtc atgagaggtt caaggattca tggaatgtta
     9541 atctaattta atagaaaagg ataattaagt atatacgttt tttcttatgt atgttatact
     9601 atgtatatac agaaagaggt gtctactatg gaaatgttac ctataaaaaa atggggaaat
     9661 agtaatggcc ttagattgcc aaagtatatt atggaatatc ttggaattca tacagaagac
     9721 aaagtgaaga taatacaaga agaagtaaac ggtcagaagc ggctaattat cgaagcggct
     9781 aatatagaaa atgaattaac aattgaacag ttatttgaaa attacactga tgaaagaaat
     9841 catgtgacga ttcaaaattt aggtgaagca gtaggtaatg aaaaatggtg agaaaaccta
     9901 gacaaggcga tattctatta ttgaatacag cacctcgatc tggacacgaa cagacaggta
     9961 aaagacctta tattgtttta agtcatgata ttattgcgga ttatagtaac gttgttacag
    10021 ttgctccaat ttctagtaca actagaaact atccgctata tgtaaatata aactcagaat
    10081 atgaaatgaa gacaacagga aaagtattat tagatcaact aacgacaatt gattatgaag
    10141 ccagagagtg tatttttttg gaaaaagcgc atgatagttt agttgaggag ttattgatga
    10201 aagttagaac agtttttcaa aaagtaagca aataaaatag aaacagcagc atgatcaact
    10261 attcgtgatt gaccatgctg tcttttttta ttctatatac ggtttgtctc gtaacattgt
    10321 agtcttttgc aatcttagcg atcgcaacac ctttttttag atcctcaacg atgtttttat
    10381 aaacaagacg acgttgggga tcttttgcat tggcactgta tagcttagga cgtcctttat
    10441 aaacaccgtt cgcttttgca atcttaatgc cttgtgcctg acgtcgttta gattcggtac
    10501 gttcttgttc agcgatcatg gccaaaattt gaatgatcaa atctttaatg aatttatcca
    10561 ggagaggatt gccaattgct tcggccatga tcggtagact tgtgatgatc agctgcacat
    10621 ttttcttctt caagtaattg actgaagcga tgatctcgtc gtaatttctt ccaagtcgat
    10681 caatagcttc cacaataaaa atatcttgat ctcggacgaa atccagtgct tcttgaaaaa
    10741 caggtcgatg agtaatcgtt gcgccagatt gtttctcaat gaaaattctc tctgcaccga
    10801 attttttcat ttcttcaatc tgccgttgtt cattttgatc gatggatgag acacgtatgt
    10861 atgcaactct caaaattttt acctactttc tgcaactatt taaaaaacac cctaatcata
    10921 cacgagaaaa ccagattttt caatgtcgcg gtcgtgtcta gttagggtat accttatata
    10981 gacaaaaact aatgatggag gaaatagtat gagaattttt tgtaagtgtc tactgataac
    11041 ttattctcaa atgttgtaag gattttattc atttgttaat attaaagaac ggaactccaa
    11101 aaaagtccgt actctaattt gcagtctctc actcttttag agtgagagtt tttattagat
    11161 tttcaaatta attagtaaag tgatagaata aattgatcat acaaatcaaa agaagaagca
    11221 gggtgatagc atacctatgc ttcttttttt atgtatttag ctatttcttc tttacaatat
    11281 cgaacatatg tttgtataat gtgaaagaat ggagtgttat ttatgatgga ttattcaaaa
    11341 gagccagtaa atgattactt tttaatcgat atgaaaagct tttacgcaag tgtcgaatgt
    11401 atagaaagaa atttagatcc attaacaaca gaacttgttg ttatgagtcg agctgataat
    11461 actggttcag gattgatatt agcttcttct cctgaagcaa aaaagcggta tggtattaca
    11521 aatgtgagta gaccacgtga cttaccacaa ccatttccta aaacactaca cgttgttcca
    11581 ccacgtatga acctatatat caagcgaaat atgcaggtaa ataatatttt cagaagatat
    11641 gtggctgatg aagatctact gatttactcg atcgatgaat caatcttaaa agtgacccga
    11701 tcactgaatc tttttacgac tgaaggaaca cgaagtcaac ggagaaagaa gctggctcaa
    11761 atgatccagg agaatattaa agaagagcta ggattgattg ctacagtagg tgtcggagat
    11821 aatccccttt tggctaaact agccttagac aacgaagcaa agcataatga aggatttgtt
    11881 gcggaatgga cgtacgaaaa cgttcctgaa aaagtatgga atattcctga aatgacggac
    11941 ttttggggaa ttggctctcg aatgaaaaaa agactcaatc agatggggat tatgagcatt
    12001 agagatcttg ctaactggaa tccttatacg attaagaata gactaggtgt gattggctta
    12061 cagctgtact tccatgctaa cggaatcgat cgaacagata tagcaatccc gccagaaccg
    12121 acaaaggaaa aatcttatgg taattcacaa gtgttaccac gagattatac tagaagaaat
    12181 gaaatcgagc tggtggtaaa agaaatggcg gaacaagtag ccattcgtat caggcagcat
    12241 aattgtaaaa caggatgtgt tcatttaaac attggtacgt cgatcttaga aacgagacca
    12301 ggattttcac accagatgaa aatacctatc acagataata caaaggaatt gcagaattat
    12361 tgtcttttct tgtttgataa gtattatgaa ggacaagaag taaggcacgt tggaataacc
    12421 tatagcaagc ttgtgtacac cgattcgctt cagctggatt tatttagtga tccacaaaag
    12481 caaattaacg aagaaaattt agataaaatt attgacaaga ttcggcaaaa atatggtttt
    12541 acgtcgattg tacatgcttc ttcaatgtta gaa
//
LOCUS       DASHRQ010000077        11628 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.081, whole genome shotgun sequence.
ACCESSION   DASHRQ010000077 DASHRQ010000000
VERSION     DASHRQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.081"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(46..1449)
                     /locus_tag="U2M88_002383"
     CDS             complement(46..1449)
                     /locus_tag="U2M88_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEM8374726.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(1436..2467)
                     /locus_tag="U2M88_002384"
     CDS             complement(1436..2467)
                     /locus_tag="U2M88_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8374727.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            2498..2719
                     /locus_tag="U2M88_002385"
     CDS             2498..2719
                     /locus_tag="U2M88_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEM8374728.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(2884..3621)
                     /locus_tag="U2M88_002386"
     CDS             complement(2884..3621)
                     /locus_tag="U2M88_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEM8374729.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(3774..5114)
                     /locus_tag="U2M88_002387"
     CDS             complement(3774..5114)
                     /locus_tag="U2M88_002387"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEM8374730.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(5351..6874)
                     /locus_tag="U2M88_002388"
     CDS             complement(5351..6874)
                     /locus_tag="U2M88_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEM8374731.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(7252..7863)
                     /locus_tag="U2M88_002389"
     CDS             complement(7252..7863)
                     /locus_tag="U2M88_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEM8374732.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(7986..8111)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(8193..8909)
                     /locus_tag="U2M88_002390"
     CDS             complement(8193..8909)
                     /locus_tag="U2M88_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEM8374733.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(8915..9484)
                     /gene="scpB"
                     /locus_tag="U2M88_002391"
     CDS             complement(8915..9484)
                     /gene="scpB"
                     /locus_tag="U2M88_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEM8374734.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(9468..10265)
                     /locus_tag="U2M88_002392"
     CDS             complement(9468..10265)
                     /locus_tag="U2M88_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEM8374735.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(10235..10606)
                     /locus_tag="U2M88_002393"
     CDS             complement(10235..10606)
                     /locus_tag="U2M88_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEM8374736.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(10625..11512)
                     /gene="xerD"
                     /locus_tag="U2M88_002394"
     CDS             complement(10625..11512)
                     /gene="xerD"
                     /locus_tag="U2M88_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEM8374737.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         3322 a         2498 c         1970 g         3838 t
ORIGIN      
        1 atcgtgtgta ttataccata ggaaaaagtg tcttgtccta aaaaatcaat gttccatgtt
       61 ttttttaaat aaatttagta aaatcgtttc ccaatgaagt tgttcagtct tagaaaaaag
      121 cttcctcttt ggcagaagtt ctgcttttgc tccatcaata tcacaacaat tctgcggctt
      181 ttcggagagt ttttcgccaa aataagctaa gatgaatttt ctgcggcagt tttcttcatt
      241 aatatagcga agcatctgct gcaggcggaa ttctttttct ttttcctgac gtttgagccg
      301 ttccacccaa ttttctggtt tttcagactg ctggatcagc tccatccatt ttttttgcag
      361 ctcatcaaag ggagcttgct ttgtggcata ttccaaataa agctcaaaac tttgtcgctg
      421 ttctctagaa agttgattaa agaaataatg gatacgttca tccccacttt gataaaaaag
      481 aattgctgca ctttcttcct gatctctgcc tgctcgaccg atttcttgta catagttttc
      541 tagactgtct ggcaaatcat aatggaccac ataacggata tcagatttat cgatccccat
      601 accaaatgca ttcgtagcaa tgagaaactg cagctgattt tttacaaact gctgttgaag
      661 ttgtcttctt tgagctgcat caagaccacc gtgatagtat ccaactgtaa aacgttccct
      721 gaataaatga tacaatcgtt cgacttcttt ttttgttgca caataaatga ttgccgctcc
      781 ttgtgctctt tccatgaagt gttcaagttc ttgttctttt tgctgcgttt tccgaacaaa
      841 aagcgagata ttttgccgat tgactgattg tctgatctct tttggtggtc gatcaaatag
      901 tacatgacgt atatctcttg ctactaaatc tgttgctgtc gccgttagtg ccagtgtgac
      961 cgggtttcct aaaagctttc ggatttttcc taactgttga tattctggac ggaaatcaac
     1021 tccccattga gacacgcaat gagcctcatc tactacatat aacgcaatat tttgtttttt
     1081 cagatgctct aaaagagaag gattagtcaa catttcggga ctcaaaaaca aaaatttata
     1141 ttgcgataga tgacggagga cgtatcttct ttcagattgc gaaagtaaac tattgaaagc
     1201 agctactcgt ttctcccctc gtttttgtaa ttgcgtcact tgatcttcca ttagagaaag
     1261 cagcggtgac acgatgatga ccagaccttc cattaaatac cccgtcaatt gatagcaaag
     1321 gctttttccc gttcctgtag gcaaaatagc gagagtatct tctccatcaa gcaacgcctg
     1381 aataatttct tcttggccgg gacgaaatga atcaaatcca aaatattttt ttaattcatt
     1441 tattaacatt ctgtatcgct tcctttctca atagatagat ttgatacaaa cgaaattctc
     1501 cataatctaa ttgtccatct acattccatt cgctaaagcg ccactctcgc acggaatcct
     1561 tatttggttc caaacgttcg attgtttctg cagagagaat ccactcgtat ggaaaatcat
     1621 caaaaaacaa ggcccactca ataagatgat ctgttactgt tcctcttttt aaatgacgga
     1681 ttgccatgat ctggtcaatt gtctgaccat tcataaacat ctgcttggtg atcatcatgc
     1741 tttgattgaa attttgctgt aataatgaat cgatcaaagc gtgccaaagc gaatctggcc
     1801 gtttttcgat ttgtgctaac aaaaggtgga tacatctgct ttgttgcaga taaacctctg
     1861 actcatcatt gttttttgcc agctgataag gaagcagccc tgttcgctca tttccagata
     1921 attggttagc taaaaaatct gctgcttctt ctggtatctc cgaaaacagc tgcaccaaac
     1981 tctcatacgc agtatcaatc aaaatttcac gtggcaattg gctttcacta agccattttt
     2041 ttagttggaa agtataaaaa ggacttgtct ctgctggaag atagtcttga atacccgttg
     2101 ccagattaga aattacttga acagcaaatt taatgagccg ccaactagtt tcccctgttc
     2161 tgccatagcg gtcaaaacgc aaaccagtat gttctatttg atgttcggac aacatatcag
     2221 cccctgcagg cgttagcttt gcttcatttt catttatttc gatccaacct tgctgcgcaa
     2281 ttttttgcaa aatttgataa aacttatcct gcttcaattc tggaaaactg ttatggataa
     2341 acaaaagttc gtgggtaaat ccataaataa gtactgaagt cgttcgtttt cccacaagga
     2401 gttggtaaag agaactaatc cgcagcttgt gtcctgatga aaatagcgat aaaataaaaa
     2461 caatcatatt attctcctta agttaggatg gttagaaatg tcactactat gtaaacttgt
     2521 acctgaacgc tgtattgcat gcggtctttg tcaaatcgaa gcgccagata ttttcgatta
     2581 tgatgaagaa ggaatcgttt tatttgccca agaaccagag gctcatcaac agttcgtgat
     2641 gccggaagaa gaaacatatg taaaaaaagc gtatcaaaaa tgtccagttc gagcgatttt
     2701 attagaaaat aaaatataaa tataaacgaa actttaaaaa cgtacactaa gttctctaaa
     2761 cttgaaaata agccgggatc tgtttctctt gttccgtgaa atcccgactt attttgaaag
     2821 attcatttgc tatctatagt taattgacta gttttttcac ggaccctata ggacccattt
     2881 atattatttc aactttttca gccagttttc caatgtattg acgatcacag tttgctggtc
     2941 tgcgttagaa atttctgcct ctccatctcc tttttgctca ccatagctgc caaaacctcc
     3001 atgatttccg ccattgattg atacatactc tgttctttcc ggtaaatact gcttagcttc
     3061 ttgataagct tcctgattca atactttatc ttttgtcgcc gtgatcgata aaacaggaac
     3121 agctgattct gtcaatgttc cttttttgtc gggataactt gccatgaaga ataccccttt
     3181 taaatgtttg tctgaatgat tcttattgac ataacgactt gccattacgc cacctaatga
     3241 atggccaccg atcacataat cggaatttgg aaaatcttct ttgaccttat ccgctttgtt
     3301 tccgcccatc actgctagat caagtggcat tttgacaata gcgactgaaa agccgttttt
     3361 tgctaaatct tgcgcccaaa tactataact ttctggctct acgagagcac caggataaaa
     3421 aataagtaat ggagactttc catgattctt tttctctcct tcaaaaaata agtaatcttc
     3481 tttgtttttt ccttctttta ccaacacttg cgcttctgac gatggttcgt acgtattttg
     3541 ctgtatatag taataaccgg ctccaccgag taatcctaaa atgatgacta agccaattag
     3601 tattcgtcta aatattttca ctttctccca ccttttctat ctgagtgacc cctttttctt
     3661 aatcaagtat gattatacct taaaaaaata agagagaaat gcaaactttc cagcatcaaa
     3721 taaaaaaaga cggaatatcc atttgaatat ccgccctttt tgtatgttta tttttattta
     3781 gcacataaac gatacacagc ttctgcataa atttccattg ctttatacat actacttaat
     3841 tcccatcgtt cattcgcctg gtgcataaag tcaggagtat cagggaacat cgcaccgaat
     3901 gccacacact ggttcatcgt acgagcaaaa gtagcgccgc ctgatacaaa cggttcagtc
     3961 atgtctccag taatatcgcg atatgtcccc aatagatttt tcactaactc actgtctagt
     4021 ggcacgtata atgaatcaag gaagtcaaat tcttcataag tcaacccata tttttctgct
     4081 gttttagtta atttagctac taaatcatct ttcttgaatg taacagggat tctcatatct
     4141 acaccgattt ttgtttgttc tggtgtgatt tccaaacttg caaagttcat cgttagctcg
     4201 ccggattgtt catcttctgt tttgcccacg acagcttcac cagttgcatt ttcttgaact
     4261 aattttccta agaaattgat tgggccaaaa tcaaatactt ctgaaagagc aattgccaga
     4321 cgagaaatag cattcactcc ttgtgctgca tctttcgcgt ggatactttt accaagaacg
     4381 acgatgcctt caccttgttc ttcgaaatcg aattcgtgtt ttttcaatgc ttcttttact
     4441 tcagaaagct tttctccaga gtaaggagct gcatcaggaa caacattcaa cgctcctcca
     4501 gcttttacag aaaactcgtt cgttcctggt cctgtcaagt aagcttgcaa taagcctttt
     4561 tctgcataaa ttaaaggaaa ttcagcatct ggcgcaaatc cttgcgtaat tccttcttct
     4621 ttttcgttat atttttctaa acaacgccat aaattttctt catctgtacc aaagataaaa
     4681 cgaatacgtg tcttgaattc cacgccagca tcaatcaaag ctttgacggc atacattgcc
     4741 gcaatagaag gacctttgtc atcttgggat ccgcgaccaa ctaaccagcc gtcttttata
     4801 gttggatcga atggttttgt ttcccaatta ttttcgtccc ctgctggaac aacgtccata
     4861 tgacacaaga taccgaataa ttcatctcca gagccaatct ctgaataacc gtaatagcct
     4921 tctggatctt taaatgttct aaatcctagc ttttcactga tttccaatac tttatccaaa
     4981 gcttcaatca cttttttacc gaagggatgt ccttgtcctg aatctgcttc atctagtacg
     5041 gaaggaattc ggatcaattc gctcaatgat tctaatgctt gttcatgatg ttgttctgtc
     5101 acaaattttt tcatgatatt atcctcctaa tatgttaaaa agggtcatgt caaattcctc
     5161 cgacacaacc tcttcttctc aaataatgac gttttaagcc tgctccatca gcaagcagaa
     5221 tgttcttaac aaatgatgaa agaaaaaaca ctcactgaat ggagctattg acttgtccac
     5281 gtgcttatcg atagccgttt atcggtttct tcattcttat gatagacctt gtacctgtat
     5341 ttgtctaggt ttagaacatt gctgctacac ctaggatcac acagatggct acaaataata
     5401 tcgcaattaa tttaccggta aatttccacc acactgaaac atcgaaacga gcaattgcta
     5461 cagcacccat tacgatagca gatgttggtg taattaagtt tactaaacca gatgcagatt
     5521 gataagcagt gattactaaa tccttaccga cgccggcaaa ttcacccata ggtcccatga
     5581 ttcccattgt tgcagcagct aagccggaag ttgaaggaat caagaatgac atagggatat
     5641 agaaaataaa tgtaataatg atgaatacac ttgaagataa tgaacttaat ccttgttcgc
     5701 cccaatgtaa gatggtgtct gtgattaatc cattgttcat aaccacttgg ataccacgag
     5761 caacagctac tacgattgct acaccgatca aatcagaagc accagctaaa aattcagaga
     5821 caaattcaga ctctttcatg ccataagtga ttgctactac gatggacatt actaagaata
     5881 acatagtaat ttctgggaag taccagtcac ctaatggaag catgctttta cctagaaccg
     5941 tacctagtac cggaaggttt gttaaccagt ttgtcaatga ttcaaaaatc gtgaaattcg
     6001 cattcaaggt tgtccaagga atcaaactga tgatcatgat accaaaagta ataaagaaca
     6061 atacattaac atgtttttgt tttttcgtga ttacttcttg acgaccgatt gcttcaatat
     6121 cgaaatgctt catatcttct ttacgttggt tgtatactaa tgattttgtt ggatcttttt
     6181 cgatctttga tgcataacga tacacatata caatagaaat agctaacaaa ataacgaaca
     6241 tcaagaatcg aagaccaata ccttcaccca tcgaaatccc aacagcttgt gaagctacgc
     6301 ctgttgcaaa aggatttacc gttgaagcta gacagcctac ttgagaccct actaatacga
     6361 ttgctacagc tactactgta tcaaagccta cagccatcat gactgggatc agtaaagcat
     6421 agaatggaat cgtttcttct gccattccgt aagtagagcc gcctaatgca aataacagca
     6481 tcaatactgg aataagcatt ttttcttttc ctttgaattt cttcacaact gaagcaatcc
     6541 cggcatctag tgcccctgtt ttgttgataa tacctaaaaa tccaccaaca accaaaatga
     6601 agaaagagat tgggattgca gcaggtgttt ctttagttag ctcccccgct ggtacaccta
     6661 gcatcccatt gattggcgcc atgaaaatat cccacagccc ttgaggattt tgttgtactc
     6721 gttcatatga tccagcgacg atgttgcctg catcgtctac actatagtgc ccagctggta
     6781 taaaccaagt aagtacagcg attactgcaa taataataaa taagaccgta tacgctgaag
     6841 gcatttgaaa ttttttcttt tttgtttgtt ccatcttctt cttcctttct tttccgttag
     6901 agacaacaat tttggctcta atgttatttt atcaaaaaga gtatcagaac aaaaataata
     6961 acacgcattt tgtaaaactt ttgttcaaaa agttcgtttt tccctattcc ctcctctttt
     7021 ttcaggtttt taaagctttc ttctttttca ttgtagcgtt tccttttttt tataaacaaa
     7081 cattttcgtc ttttaaaagg agaagaattt cacatttgca aagaattgat tgatttttat
     7141 gcataaatca aaaaaatact caaaacaaac aaataaaacg tttacattat tgcatgtctt
     7201 gtattttttt ccaaaaaaaa agaccaagaa atttcttgat ctttttgata tttatttgac
     7261 tgctgtatga cgttgttcga actgatgttg ttttcgactt aaccatggaa gaagttgcgc
     7321 atgcagcact aaaaagacag cactcacgat gaatcctttg atcaagttga aaggaacgat
     7381 cccaatcagt acatagtttg ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa
     7441 aaacattaag taaagaggcg taataacgaa ataatttgcg atactcataa aaatagttag
     7501 agcgatggtt cctgtaatga cacctaaaaa tttgtttctt cctttatgct gtttctttac
     7561 actaaagaaa taataaatcg ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc
     7621 tcctaccaaa ttatccggag aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa
     7681 cgctgttgca actccggccg ctggaccgaa taagaacatg ctgatcaata ccgagatatc
     7741 actaaaatcg atcttcaaaa aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa
     7801 aactaatgcg atcgcagcaa acatcgctac tgcaaccatt ttctgtacac gggtgttctt
     7861 catttgaaat cctcctgtta ggactcctct tcagcgaact ttttgacgtt tctctccaaa
     7921 tgaaaaaact ctattttcca gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa
     7981 ataagctcta tcttctttat ccagactata ctgtcggtat cggaatttca ccgattcagc
     8041 tatttttttg caaaataggt cgtggactat aaccaccggt cgggaatttc accctgcccc
     8101 tgaagacgaa ccttatataa tttttgttta catattcatt atacatatct attcaaaaaa
     8161 cgcaacatct aacacttata aaaagtaagt gttcattttg agtggttcag caaactgctg
     8221 acttcttttt tgttcagttc acggtaatcc ccaggacgta ggccttgtaa agtcagatct
     8281 ccgtattttt cacgttttag tttttgcaca ggaagaccaa ctgcttgaag catatttttc
     8341 acttgatgat tacgtccttc gtggatcgtc aattccacga tactgtggtt tgtcttagga
     8401 tccactgata aaatttggaa tttagctgga gatgtttttc gtccttcgat ctttattcct
     8461 tttgtcaaag gtgcaagcat cgattttgta gcgatccctt ttacctttgc aacgtaaact
     8521 ttatccactt catgttttgg atgtgtcagg cgctgagcaa aatctccatc atttgtcagt
     8581 agcaagatcc ccgaagtatc gtaatccagt cgacctacag gatagatacg ttcttttacc
     8641 atcggcaaaa gatccgtaac aacttttctt cctttgtcat ctgcaacagc agaaataact
     8701 cctttaggtt tgtacaataa atagtaaccg tattcttctt gataaattgg gacttcatcc
     8761 acttccacag tgtcatgttt cccaactttt atccctaatt cccttacaac ttgtccgttt
     8821 acttttacac gtcctgcaag gatgaattct tctgcttttc gacgagaagt gatgccagcg
     8881 tgggcaatta ctttctgcaa tctctccatt acaattattc tcctttattc attccatcaa
     8941 agaatagatc tggtgacacg tcttcttcaa gtgaaatttc catttcttgt gtatcaggca
     9001 gttcttctaa tgatttcaaa ccaaaataat ctaagaagta gccagttgtt ccatataata
     9061 tcggccgacc tggtccttct actctacctt tttcttcaat cagtttatgg gcagctagac
     9121 gctgtaccgc tcctgaaacc tgaactcctc gaatctgctc gatttcagct cttgtgattg
     9181 gctgtttata agcgatgatt gctaaggttt cgacggcggc ttgagataat gtgtttgatg
     9241 tcccttgcgc atatcgctta agtaagggag ccagctcttt tttagtcgtt aatacataat
     9301 ggctttccgt ttcaaaaata tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta
     9361 attctttcac taaacttgtg acttctgctt cgctgagatt taacagtccg cttaattctt
     9421 cattactgat cccttcctcg ccaacaataa aaagaagggc ttcaagttca cttaatttcg
     9481 tcatgttcgg tatctcctac tgctggataa agcataattg attcatagtt ttctttttgt
     9541 tgcgcaatga tctgcccttt tttcatcaat tctagcaaag ccataaaagt agtgacaact
     9601 tcacttttag aaggtgactc aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt
     9661 atacgctctt cgataaatac cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt
     9721 ttttccactt tttgagaaaa tttcttacgt tctaagattt tctgcatggc acggatcaaa
     9781 tcgatttttt tgacttgtcc tcgcttcaac ggcttttcag catccgctaa cccatcgatg
     9841 ttcataggtt cttttgtgta ataaagactt cgttcctctg ctttttctgt cagtctttca
     9901 gctgcatatt tatatttacg gtattctaaa agctgagtga ccaattcttc tcgaggatct
     9961 tcttcccata attcttcatc gtctgtgccg aattcttgga caggaagcaa tgtcttgctt
    10021 ttgattgcca tcaatgtagc agccatcact aagtattctc ctgcaacagc tagctcgagt
    10081 gttttcattg tatggatata attcatatat tgctccgtga tcgtagcgat cggtatatca
    10141 tatatatcga tttccatttg ctgaatcaga tgaagcagca gatcgagagg accctcgaat
    10201 acatctaatt taatattgat ctccgtcaac tcattcactc tttcttcttt ttctttgcta
    10261 gccattttcc aatgtaaggc tctgtgattt cagttgcacg gatggagaca tccgggtact
    10321 tttcctgtaa ttcatctaac atttgaaagc ccatattctc accgcgataa gacggattca
    10381 aagaaatgtc atggatgatc agctgatcgt tcgttgccca gtaaatacca ataatccctt
    10441 gaatgttggt tgaaccttct ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt
    10501 acgtatcaat ctccttcaac aaagaagttt tacctgtcag tttcttatgg aaactcaata
    10561 gtcccatagc aattttacgg ttttctttcc ggtaatgtgt taacatcttc tcaccttcct
    10621 atgactaagc ccttggaaaa tgctgcttat aaacatccgt cattcttttc ttcgtgatat
    10681 gtgtataaat ctgagtcgta gagatatccg cgtgacctaa caattcctgt actgtccgca
    10741 aatcagctcc attttctagc agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta
    10801 cttctttgta aatcccagcc tctcgtacta actgcttcag atttttccag attccttgcc
    10861 gagaaagacc tttcccatga ttattgacaa atacatgcat ttcagatgcg tcctttacaa
    10921 gtaatggacg tgcttcgtct aagtagcgtt ccagccattg gatcgcataa tcacctaaag
    10981 gaatgatccg ctctttatct ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa
    11041 gatctcccaa tttcaatcca atcaattcac tgacgcgcat cccagtagca tacatgactt
    11101 ccaagattgc acgatctctt atccctaacg tttttgtcgt atcaggtgct tcaatcagct
    11161 tttctacttc tgtcagagtc aatgtacttg gtaatttctg tgctttcttc ggcgtatcga
    11221 tatgttgcat cggatcatga tcagtgatac gctcttgccg taaaaactga tggaatcttc
    11281 tgagacttga gatcatccga gtaacagttg ctgtcgcttt tttttcttca tgcaacttct
    11341 ccagaaaacg gatgactaaa aagcgatcga catcctgcca tgaagcaatt ttctgctcct
    11401 gtagaaaatg caagtactgt tccaaatcac gttcatagct ttgtcgtgta ttggcggaca
    11461 atccccgttc aatattcaag tagtggagat aatcgttgat ttgctctttc ataataatcc
    11521 ccctcatgcc tctctatcat atcagatacc gcttcattct gatagtcttt ttccataatc
    11581 tgagcaaaga aaaagccgtc tagacactca gacggactgg tttctttt
//
LOCUS       DASHRQ010000078        10976 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.082, whole genome shotgun sequence.
ACCESSION   DASHRQ010000078 DASHRQ010000000
VERSION     DASHRQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.082"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(55..1431)
                     /locus_tag="U2M88_002395"
     CDS             complement(55..1431)
                     /locus_tag="U2M88_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEM8374738.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(1559..2677)
                     /locus_tag="U2M88_002396"
     CDS             complement(1559..2677)
                     /locus_tag="U2M88_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEM8374739.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            2819..3253
                     /locus_tag="U2M88_002397"
     CDS             2819..3253
                     /locus_tag="U2M88_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8374740.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(3331..3864)
                     /locus_tag="U2M88_002398"
     CDS             complement(3331..3864)
                     /locus_tag="U2M88_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEM8374741.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(3958..5136)
                     /gene="mutY"
                     /locus_tag="U2M88_002399"
     CDS             complement(3958..5136)
                     /gene="mutY"
                     /locus_tag="U2M88_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEM8374742.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(5129..5926)
                     /gene="recX"
                     /locus_tag="U2M88_002400"
     CDS             complement(5129..5926)
                     /gene="recX"
                     /locus_tag="U2M88_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEM8374743.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            6033..7415
                     /gene="rlmD"
                     /locus_tag="U2M88_002401"
                     /pseudo
     CDS             6033..7415
                     /gene="rlmD"
                     /locus_tag="U2M88_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
     gene            complement(7589..8167)
                     /locus_tag="U2M88_002402"
     CDS             complement(7589..8167)
                     /locus_tag="U2M88_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEM8374744.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            8489..8959
                     /locus_tag="U2M88_002403"
     CDS             8489..8959
                     /locus_tag="U2M88_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEM8374745.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(9067..10293)
                     /locus_tag="U2M88_002404"
     CDS             complement(9067..10293)
                     /locus_tag="U2M88_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEM8374746.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         3274 a         2190 c         1843 g         3669 t
ORIGIN      
        1 aagacgtgtt gcttgtttat tcaagcaaca cgtctttcat aaccaagttt ccaattatct
       61 tcttggctcg tcatcttcga attcttttct gaagtatctt cgttcttctt caggttccgc
      121 ttctgtttct tccggatcat gaaaaatgac acggatttta aggattcgag aaccttccat
      181 ttcttcagaa accagcgtaa tattatcgac atcaaacgat aacttttcgc cttcatccgg
      241 aatcatgcct aatgctgtaa tcaaataacc agccatcgta tctacatcac tcatatgcag
      301 atcactgtca aaagcttcat tgaactcatc aatcagcata cgtccttgga tgatatattc
      361 gtgttcatcg attttttcat aaagattctc aacttcatct gattcatcat cgatttctcc
      421 gacgatttct tctaaaaggt cttctagtgt tgctaaccca acgacgccgc catattcatc
      481 taataaaata gccatctgat tctgcgtctt tttcatttca tataacagat catcaataaa
      541 gaccgtttca ggaacgaata aaggctcctg catgattttc ttgatatcta aattatcaaa
      601 gccgaattta tgggcagctt ttagtaaatt ctttgtatga aggattccga ctactttatc
      661 tttgtcttcg ttataaacag ggattcttga atagttttcg gataaaactt cattcacatt
      721 ttcttccaca gtgtcgttga tatcaatcat aaatgcatcc gttcgtggga ccatgacttc
      781 tcgggcaact ttcgtatcaa gagaaaaaac cccttggagc atttctaatt cttcattatt
      841 caatacgcct tcgttttcaa gcatataacg catttcatct ctggtcattt ttgaatcttc
      901 atcatcaaaa gtcatcggcg tgatacgcgc caataaactt gtggaagcag aaagcagcca
      961 gacgaagggc ttagctacta caccaatcaa gcgtatcagc cctgaagtga actttgcgac
     1021 ttcttctgat ttatttaaag caatacgttt agggtacaat tctccaaaaa caatcgatac
     1081 ataggtcaaa atagccagca caatgatatt tgcaatattt tttgccgctg tgccgccacc
     1141 taataccgga gctaaccgtt ccgataaagt attagctagt gaagcaccag acaaaatgtt
     1201 gaccaaagtg attcctactt ggattgtcga taggaaatta tttggattct gcaatatttt
     1261 taaaagcttt tgagatttca catctccttc atcagctttt tgctcgattc gattcttatt
     1321 gatcgaaaca acagcaattt cggaagctgc taaaaaagca ttgatcaatg tcaaaacgat
     1381 caataataaa atctgcgcta acagcgactg actctcaggg tcagcattca taatcgcatt
     1441 ctctccttat aaaattaatt tttagtttac gatagaaaaa aacgtggcac tatagccacg
     1501 ttaaaatggt atcacaaagc aattttcttt gcaataaaaa caagtgtact aaaactacct
     1561 acgaggtcgt agcaattttc ttcttgtttt cattttgctt gtcttctttg acgattttga
     1621 ttatatagga aatgatcgtc ttgcagattg cgtaaaaagg aacacctaag aagataccaa
     1681 gcaatccggc aatatttcca gctactagaa gaacgataat gatcgttaat ggatggattt
     1741 tcagtgattt cccaattaca ttaggataaa tgatattccc atccaactgt tggacgatta
     1801 gcacaaccac acaacaaagt aatgctttta ccggatcatt gaagactgtt actagaaatg
     1861 caggtgctag tcctagataa ggtcctaaat atggaattag gtttgtcaat cccgcaatga
     1921 ccccaaataa gaaagcataa tccacaccaa ttaccatata tcctaaaaat gtgaaggtac
     1981 caacgaataa acattcgatt gcttgtccgc taatgtaatt agccaatgtt ttattcagtt
     2041 gccccaataa ctccacaatt tggtttcttc ttttttctgg gaaaaactgt tggatgtttg
     2101 gcactagttt ttctccgtct ttcaacatat agaacaagat gaatggtgct gtaatgatta
     2161 ctacggttgc cgaagcgatc gttccaacga tcgaacctaa actattggat actccactaa
     2221 ggaattgctg aatgattgtc ccgtaagaaa tgtctaattg ctcaaaatat ttattgatat
     2281 ctacttcctt gaacaaaggc aattcggcta cctcttttaa ccagctttct acctgtttga
     2341 tgaaatcagg catattagat gccaatgagg aaatttgctg tactagactt gggatgacac
     2401 taagtaaaat ccaaataatt gctgcaacta aaaggagcag cacaattgcg acagcccata
     2461 ttcttttgat ttttgctttc atcaacagct tgacaatagg attcaataaa tagtaaagaa
     2521 agcctgcaat cagtacaggt gcaaataacg tggaaaaaaa tgtcccgatc ggttcgaaaa
     2581 taaagtttat ttctgatgaa atcatgatca atgcagctat gatcaataat tcaagcgacc
     2641 aaaacattag tttagactgt ctaagttttt caaacaatag atttcctcct ttctttctta
     2701 ctagtatata ttatattcgt taaaaattct tttattcttt ttttattttt gaaaacttgc
     2761 catacttttt atttgtttta ttttccagta tactatacga aaggaaggtg gctagaccat
     2821 gaaagttgta caaacaaaag atacaatgag caatatttac ttagatgctg tacggatcag
     2881 gcatcaagtt tttgtagtcg aacagggtgt gcctttgtct cgagaaatag acaaagatga
     2941 agcccattgt atccacttcg tcctgtactc agataaaaaa gaaccgcaag gaactgtacg
     3001 tcttttgcct ttagaaaacg gaaaaatgaa attacagcgg atggcgattc tttctgaata
     3061 cagacaccaa gggttaggaa aaatattgat tgaagaagca gaaaactttg ctaagaatca
     3121 aggctataat acgattttat taggggcaca gtccacagct gaaacttttt acgaaaaact
     3181 aggatataca gcttacggcg atccttttga agatgctggc atgcctcata tttatatgaa
     3241 aaagacttta taaaaattta tttcgaaaaa agcaaaaaac agcaagcttt tgagaaaaat
     3301 ctctcactgg cttactgttc ttttttgtgt ttacttacgc gacaattgct tgtaacggtt
     3361 ataccaaata tcaatatagc cttccgagaa cgggccatct ccattattga tccagtcaac
     3421 cagaatcttc acattttctt ttaggatgaa gtcaatatcg ttcgggtaat tcatcatttt
     3481 gctgtgcatc tcatattcat cgacatccaa tagtcgtttc tcgccgtcag gaaatacctt
     3541 gatatccaag tcatagtcga tatatttcaa ggcctcatcg tccagcaaaa aaggtgaagc
     3601 tagattacaa taataggaaa cccccttttc tctgatcatt gctattacat tgaaccagta
     3661 ttttttatga aaataaacaa tcgctggttc gcgcgttacc catcggcgtc catcagattc
     3721 agtgactaat gtgtgatcat tcacgccgat catagagtgt tcgcttgttt ttaataccat
     3781 ggtatctcgc cacgttcgat gcaatttgcc gtcgtgcttg taactttgga tcgtcacaaa
     3841 ctctccttct tttggaactc ccatagcaac ccttccttaa gcagcatttt gtgctttgtg
     3901 tttgggtgtt cattacaccg acatcataat tattatatca ataaaaatga gctttgccta
     3961 gaaaggattc ctattgtttc gatttttctc taactgatct accagtttca tttggacctt
     4021 aggaaaaacg accgaatcaa atgcatcagg cttcaaccac ttcgaagttt tgttctcggt
     4081 aaattcttgt tctgctccct cggttgcccg tccgtaaaaa aggagaacat gccatttcag
     4141 atgactaaaa atatgagtaa cttctcctaa atgtcgtgtc tgccaaacaa agagttcctg
     4201 cttttcaaat gggagatttt gctcttcttc tgctactagg tcatcaaaca gatcgatttc
     4261 tggctttgtt tcccattctt ttttcaatcg ctcatactcc tcttgtgtca cttccatcat
     4321 tggaaatgtc cacatattcg ccagcaattt ttggctatct ctttcttcaa aataatacgc
     4381 tccagaatga ttttgaagag ctgcactgat ataataaacg tcttttggct ttgctttttt
     4441 tgtttttaca ggaaaactcg tttggattcc tcgtttgtta gctaaacaaa aggcttggat
     4501 cggacaagtc tcacactttg gagaagttgg tgtacagata gctgaaccta gatccatcat
     4561 tgcttggttg aattcacctg gatgcttttc atcaatgatt ttccgcatcg cttcatcaaa
     4621 gattttcctg ctagaagctt ttgcgatgtc tgcttcaatg caaaacaatc tgctgactac
     4681 tcgcatcacg ttgccgtcaa ctgcaggttc tggaagtcca aatgcgatac ttgcaatcgc
     4741 gcctgtcgta tatggtccta ttcccttcaa tgagctaatt tcttcaggcg tttgcggcat
     4801 ttccccatca aattctgaca tgatctgttt agcagcagct tggatgttcc gggctcttga
     4861 gtaataacca aggccctccc atgcttttaa aagcttttct tctggagcat ttgctagctc
     4921 ttcaattgta ggaaaccatt ccatgaaacg ataaaaataa tcgatcactg tgtctactct
     4981 ggtttgctga agcatgatct cagagatcca gatacgataa ggatcacgat tatagcgcca
     5041 aggcaggttt cttttttctt gttcatacca ttgaatgaac tgatcttgaa attcttttgt
     5101 ttctttatct gtccattttt cccattcttt attcactttc ctcttccttt tccgtaatga
     5161 atcgctggat catatccaaa tcaaatcctt tttgatataa gctttgcttt acttttagat
     5221 ttcttttttt aggttcaaac cttaaatttc ttcgccatag tttatctcct tgtttgacta
     5281 aataatcata ttctatttct tgatctgctt cttgcggaag atcagctatc gcttgttgga
     5341 tcacttcttg agaaaagcct ttcgtcatca atgtttgttg gatttttctc aataattctt
     5401 tttggctttt tccgtgattt tttccaaacg ctttttcggc tgtctgatgt gcattttgaa
     5461 cttggtcttc aaatgtatat tggctgagcg cttgttcaat gatttcaggt ttcaaacctt
     5521 tttgctgcat ctgctgcgct aatttctttg gtcctttgtc acttaagcgc atattcgtgc
     5581 gcacataact ttccccatag accaaatcat cgacaaggtc caattctttt aagcgggcta
     5641 cgattttatt tcgatcttct tgaaggattt ccttttcttt taagtatgtt cggatttctt
     5701 tttccgatcg caactgataa cttaagtaat tcattgctag gtgaaaaccg aaatcagctc
     5761 cgctactctt cttgatctct tggatagttt cttcgtctaa ttcccgtcct tttaataatc
     5821 gatagcgtac taatacatct tcagatactt gtaaagcttc tccttcagat aaatctacgc
     5881 gatagaaagg gcctttgcct ttactgattc tcgtaacggt taacataaac atcctcctcg
     5941 aacatatatt ctctatcata ccacagaaaa tccccgtaat caggtattat atgttagaat
     6001 aaaccacaga aaaaagaaaa agggagatac ttatggcaga aacaattgtc aaaactggac
     6061 aaacggttcc tttgaaaatc aagcgtttag gcataaatgg tgaaggaatc ggttatttca
     6121 aacggttgat catatttgtt ccatatgcac taccaaaaga agaagtacta gtgaaaatca
     6181 cgaaagcaac tcctcgctat gcagaaggag aattaataaa agtcaaaaaa acaagcaaag
     6241 atcgcgtagt ggcaccatgt cctgtttact atgaatgcgg tggctgtcaa ttacaacact
     6301 tagcttatca agctcaattg gattttaaaa aggatctgct tttgcaagca ttggaaaaat
     6361 tcaaaccagc aggctttcga tcttatcaac ttttgccgac gatcgggatg gatgagcctt
     6421 ggcattatag aaacaaagca caatttcagt tacgaaaaaa taagcagact caaaaaattg
     6481 aagctggttt atacgaagca aactctcatc agttagtccc tataacagac tgcttggttc
     6541 aagaacctcg aacacaaaaa gtaatgaata ctgttgttca actattgaat aaatttgatg
     6601 cacccatcta tgatgaacgg acaaacagtg gaatcttccg cacaatcatg gttagaatcg
     6661 gcgttaaaac tggggagtta caagttgttt ttattactcg cagtaaaaaa ttcccgcaaa
     6721 aaaacgcaat gatccgtgag atcaatgaac gactgcccga agttgtttca atcatgcaaa
     6781 atgtccaggc acaaaaaacg tctctcgtga tgggggatga aacgatccac ttatggggaa
     6841 aagaagccat tgaagaacaa atcaatgaag tgactttcga tctttctcct cgtgccttct
     6901 ttcaactcaa tcctcagcag accgaattgc tttatggcga aggaatcaaa gcattagatg
     6961 tccagcctaa tgaaacgatc gtggatgctt attgcggagt tgggacgatc ggcttaagtg
     7021 tagcaaaaca agccaaagaa gtacggggaa tggataccgt tcctcaagca atcgctgatg
     7081 caaaaaaaaa tgcggaaaga ttaggatatc acaacactcg atatgaagtc gggacagcag
     7141 agcaagttct gcctaaatgg ttgaatgaag gttttcagcc tgatggtatc atcgtagatc
     7201 caccacgtac gggtttggat aacgcattga tccaatcatt attgaaatac cctccaaaat
     7261 aacttgttta tatctcctgt aatgtttcta cacttgccag agatctggtt aatttaagta
     7321 aaaagttcaa gattgaatat ttacagtccg tcgacatgtt tcctcagact gcacgttgtg
     7381 aagttgtcgt gaagatgacg agactcgctg actaaatgaa agaggttgtg aaaaagtctg
     7441 ccctgtatca agtaataaga acagccaaac aaaaatagct cttcatgttt tatgaatact
     7501 gtacttgtga ccgaagagtt aacaggagga cactatttat ttaaaaaaag ggttgtgaca
     7561 tagtttttgt cacagccctt tttgattatt attggatatt cacgtctatc acttgataaa
     7621 atgcatttgt cgtatcgctg atattccata ctgctaaaat cacattatat ccttttcgat
     7681 cctgcggaag tgtgatcgtt tggttgactg tttttggtgg aacagttgct ttgtcgtcta
     7741 tttttgtgat caattcaaaa tcactaaatt tcaaaggttg attaggattc caagttggtt
     7801 tggtaatgta gtaatcccaa gtacttgtcc gatgttgtgc tgttaaattc caagtaatat
     7861 ttaatgttcc tggattaacc atgtttttat gccatctgtt agatgtctgt tcatctaaag
     7921 gctcaaagcc gctgactcct gcactagcaa tttttccatc aatgaatgta tttttggtag
     7981 cttcgatact ttgcggttcc cattgtgctc ttccgacatt ttgatttaag tttcctgctg
     8041 ctgatgttcc taggtatgca cggcttcctg gagaagtaat ataaccatgc gcaaaacctg
     8101 gaatgctaaa accgaacaac cctaaactaa ctaacccaat aaacaataac gtactaatct
     8161 ttttcatgta gaaacctcct tcatttgata accacattgt aaacgcttta ttgtattaac
     8221 acaaacatta tgcttttata attttatgtt gaagtaaaaa aggtgaaaaa agtaagatgc
     8281 agattataac agaagtagac gagaaaagta caaatagaaa acatgagaat caaatcatat
     8341 gatctttttc aaaaacatca ttcatataaa caaatgttca actttttaat aacaacaatt
     8401 atcaattttt ttcttacttc acaaactaaa tacaggtctt gctgtgagcc ttcatggtaa
     8461 actaatatta tagaaaggag ttagtaaaat gaagatgata aaagatattc gtttttgggt
     8521 ttgtgttata ggaattgtca tattaggctt tttatcaggg ttgcttagcg gaaatcccgg
     8581 aaaatattac tattctttgc aacttccacc atttgcacca cctagctgga tatttgggcc
     8641 gatgtggacc ttactgtata tattgatggg tatttctctt tatttgttac ttaatcacaa
     8701 caacaaaaaa cagcgaaaca atcttgtcgg tctttttgtg attcagttca tttttaattt
     8761 tatatggtct gctctatttt ttaatttaag aaatattttc attgcagcaa tcgatattac
     8821 acttttagtt attttcttgt cagtgcttat gtatcagttg tggctgcatc atcgcctggc
     8881 aatgtggctc atgattcctt attatttatg ggtactattc gcaactctat tgaattattc
     8941 aatttatttt ttaaactaag cgctaaaaag agacgtcctg taatttaaca catagaaaaa
     9001 gtgccaccta agttagattt tttgtctaat ttaggtggca ctctttatta accaaactat
     9061 ttttttctag tacccacttg ttgttccata ctcattattc tggtcataac cataaccatt
     9121 ttgttcctgt ccagcgtaag gatcataact ttgagtatct tgacttggtg tatagtactg
     9181 acttgtatct tctgtttgct gataataggt attcggatct tggtactgat catatgtgtt
     9241 ttctgaatag gaagaataat cggtgtaccc ttcactattc gatccatcac cgtttgtcga
     9301 atcgccatct tccgaatcat caaagaacat cattgaagca ttctcatttt tcaaattagg
     9361 taatgtatcg cttgttttta tatttaattg tttcttcagt tcattttgga tcgaaagaag
     9421 atcatttttt cctaaaattt gatagtagat atcattgatc atttgtcctt cgccttgcaa
     9481 ctggtcttgt ttgattgttt caaacgccgg ggtgtagttc gtcgctacat caagcatgtc
     9541 atcccaatct aagtccgtcg tcacattgtt ctcaactgct tttaaaattt ttctataatt
     9601 gcttacgcca tctaaactta aaactttatt gacgattttc gtcactactt cccgctgacg
     9661 acgttgtcga ccgatatcgc cttctggatc ttcgtgtcgc attcgagaat aggctaaacc
     9721 tttttctcca ttcaatgttt gttcacctac tggtacatgg acaccatcca aagtaaagtc
     9781 aattttatta ttcactttga tgccaccgac cgcattgatc aaatcttcca tgccatccaa
     9841 attgatcgtt acataatgat caataggaat atcgagcagt tgctcaattg aatccatggc
     9901 catttcgaca ccaccaaatg cgtaggcatg atttaattta tctaccgttc cataacctac
     9961 gatattcgta taaatatctc ggtccaaact gatgatcgtt gatttttttt gtttcggatt
    10021 aactgttact accatcatcg tatcagaacg tccttgttcc gttcttccta atccaccggt
    10081 atctaaacct aatagcaaaa ccgaaaaagg ttctttgtca tcaatgctta ctctctcaga
    10141 acgtttggag gatattcgat tagagttttt agaaattttg tttactgttt ggctcgcttc
    10201 tcccataaat ctgattccgt atgctgataa tccggcaatc agcaaaacga atacccctaa
    10261 caaggttaat agaacttttt gccatcgctt cataatacac ctctgtttca cattttctaa
    10321 aatattgtct catataactg ttcaatcagc aaattcactt tttaatcata agaaaacttt
    10381 aaaaaacatt tttgtgtaac gattattatt ttttaaaatt tctcaaaatt cttaactatc
    10441 ataccatacc gtgaaaaaga tcgaaagacc gaatacacgc agttcgcaat cgttttatct
    10501 ttttttatgt attttttcca gaaaaagcga acaacaaggt attttccaaa taatcgtgca
    10561 gtaaaatgat cgcacatacc tttcccatct ttttaacagt ctttgcctac cgttcagatt
    10621 atcattcgat ttcacgacaa tttcttccta tgactggtcc ttttgttaaa cccatcttct
    10681 attcgtttta tttccagctc actgcatggc tcttaaaatg actcgaacta gtgattgcat
    10741 taccatcatc ttttaatgga tagatatgct cctcttcaaa gtaaaacgat tttcttttaa
    10801 agtaatagaa ttgacatggt aatgagtatg tatactagtg gtttcacaaa tggtctttta
    10861 ttcaacatcc aaaaactgaa atttctttaa aatccattac taacgaagaa aatgttcctt
    10921 gcttgataat tagttaggac atgtcaagtg aacaaatgca tttacttagc caaaaa
//
LOCUS       DASHRQ010000079        10552 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.083, whole genome shotgun sequence.
ACCESSION   DASHRQ010000079 DASHRQ010000000
VERSION     DASHRQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.083"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(25..162)
                     /locus_tag="U2M88_002405"
     CDS             complement(25..162)
                     /locus_tag="U2M88_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEM8374747.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="U2M88_002406"
     CDS             complement(159..1343)
                     /locus_tag="U2M88_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEM8374748.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="U2M88_002407"
     CDS             complement(1599..1853)
                     /locus_tag="U2M88_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374749.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="U2M88_002408"
     CDS             complement(1878..3020)
                     /locus_tag="U2M88_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEM8374750.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNGTEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="U2M88_002409"
     CDS             complement(3080..4432)
                     /locus_tag="U2M88_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEM8374751.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="U2M88_002410"
     CDS             complement(4503..4847)
                     /locus_tag="U2M88_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEM8374752.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="U2M88_002411"
     CDS             complement(4857..5231)
                     /locus_tag="U2M88_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEM8374753.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="U2M88_002412"
     CDS             complement(5244..5558)
                     /locus_tag="U2M88_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEM8374754.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8899)
                     /gene="fss3"
                     /locus_tag="U2M88_002413"
     CDS             complement(5672..8899)
                     /gene="fss3"
                     /locus_tag="U2M88_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEM8374755.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(8978..9640)
                     /locus_tag="U2M88_002414"
     CDS             complement(8978..9640)
                     /locus_tag="U2M88_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374756.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10257..10487
                     /locus_tag="U2M88_002415"
     CDS             10257..10487
                     /locus_tag="U2M88_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374757.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
BASE COUNT         2901 a         2346 c         1792 g         3513 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgttccgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccagaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcatctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtgtagcca ttaacttctt gccaaatcat cttctgggcc acaatatgtg
     8521 tatccacatc cgtaccagct tccttccata aaacggaaac taatttcgct ttctccgaca
     8581 tatcaggcaa tggatttttc tcataaccag gagtgaactc attgtaaatc ggcacccctg
     8641 gctcaataca gaaaatatct tgcttcacac cattgtaaac tgcatagtgc ggaacggtaa
     8701 cttctgacat gggacgacca tcgctaaatg taccttcgac agcatactgg tgagccaaat
     8761 ccaattccac agttaccgtt tgtggatgag taatttcatc agcaaaggca atcgttgggc
     8821 caagcgatcc accaattgtt tggcataata gactcagtgt tgcgagcaaa cgccaattct
     8881 taaatcttgg ctttttcata aaataaatct cctttcgttt ttgggtaata aaaaagacgt
     8941 ccaaatttta attttgaacg tctgatttag agtgatacta tttagttttc tttgtcttgg
     9001 ataggttgtt tctctgctta taaattttga ttcttcgata atcaaatcca atctctgcca
     9061 tcaattcctc ccaatttttg ttcgttcttt tcataattgt atcaactgat ggtagttttg
     9121 gggattgaac tagttttcga aactttgtca ttgataaatc atcctgtagt tttaatcgga
     9181 ttatttcatt ttttagttct aacagcagtt cgaaatcagt aaatcgagat ccatcatttt
     9241 tttctttaaa aagtggacgt atatctccta acatcttttt aatggtacta agtgaaggaa
     9301 tattcttccc tactgatttt tgttcgtaca ttcgttgaga ggttatcttc tcgcacttaa
     9361 taaaggcttc tactatttcc aataattctt gttctgacat tctagcccat tttccatacc
     9421 cagtattttc tcgtcctata ctaatcaata aattctccca tgatccatat tttttgttaa
     9481 tgaaccacat acttggtgca gaatttggat tttctgatag ctttttctgg tattgacggc
     9541 tagatgtaat tccgtgtttt tcgatgaatt cattaattgt taaaatcagt tttaactcac
     9601 tttgattttt ccaaatgata cgtccatcat tttgcttcac gataaaaact cctaatagtt
     9661 atttagatag aagcctatca catcaacttt ttagtagaaa agtacatgtt ttgtcttttt
     9721 tgctaatagc tgaccctttg agaaacttgg gttcaaaagt tgctaaattc ggtaaatgtt
     9781 ttggtatttt tcgtattata tttgtcgtca agctgactac taaattttgc cctattttga
     9841 cgacactgat agtcaatgtc ggacaatcta ggacaatttt taagggaata tgattgaaaa
     9901 atggtccaaa acagccaaaa atggcttaaa actgtgatag agaaaaaaga aaaagccctt
     9961 gatatacaag gacttttcac aaatttcaga tttccaagga agttttccgt gtttatcttc
    10021 tgatttcttt gatacgagca gcttttccgt gtaatgcacg taaacaaaac cactatattc
    10081 tattatgtat ctaataccct caaaacagct ataaatcaac aataccgaca tataataaac
    10141 actatgttca atctgagata tatcaaaggt aaacgtattt ttgccccctt tttgcccctc
    10201 aaatatcgct ttacaacgag aacgtaagtt cgtataatcg ttttgaggtg atttttatga
    10261 tggaagactt tattagaaaa aatatcagtg atgaatatgc agacttttat gagcaaagca
    10321 gcaaaaaaga taaattccag atggatgttt caatcttagc tatattagct ttttccgaaa
    10381 ataaccaacc tgtaattgcg aaaaaagaaa cagtattatc tgaaggcaaa ataaaaactc
    10441 gatatatatt agaggtagaa actaagttta aaaatagatc ggagtaatgg ctatgctttt
    10501 taatgagaca caattatggt ttaaattcga tccttcgaat agatttgtca aa
//
LOCUS       DASHRQ010000080        10437 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.084, whole genome shotgun sequence.
ACCESSION   DASHRQ010000080 DASHRQ010000000
VERSION     DASHRQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.084"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            91..309
                     /locus_tag="U2M88_002416"
     CDS             91..309
                     /locus_tag="U2M88_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374758.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(598..1110)
                     /locus_tag="U2M88_002417"
     CDS             complement(598..1110)
                     /locus_tag="U2M88_002417"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEM8374759.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1124..3421)
                     /gene="recJ"
                     /locus_tag="U2M88_002418"
     CDS             complement(1124..3421)
                     /gene="recJ"
                     /locus_tag="U2M88_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEM8374760.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3563..4006)
                     /locus_tag="U2M88_002419"
     CDS             complement(3563..4006)
                     /locus_tag="U2M88_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEM8374761.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4007..4795)
                     /locus_tag="U2M88_002420"
     CDS             complement(4007..4795)
                     /locus_tag="U2M88_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM8374762.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVGNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4792..5733)
                     /locus_tag="U2M88_002421"
     CDS             complement(4792..5733)
                     /locus_tag="U2M88_002421"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEM8374763.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5910..6497)
                     /locus_tag="U2M88_002422"
     CDS             complement(5910..6497)
                     /locus_tag="U2M88_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEM8374764.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6718..8025)
                     /gene="obgE"
                     /locus_tag="U2M88_002423"
     CDS             complement(6718..8025)
                     /gene="obgE"
                     /locus_tag="U2M88_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEM8374765.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8319..9329
                     /gene="gap"
                     /locus_tag="U2M88_002424"
     CDS             8319..9329
                     /gene="gap"
                     /locus_tag="U2M88_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEM8374766.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9414..10349)
                     /locus_tag="U2M88_002425"
     CDS             complement(9414..10349)
                     /locus_tag="U2M88_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM8374767.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2888 a         2357 c         1808 g         3384 t
ORIGIN      
        1 ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata tacgaacaat
       61 tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct tggcttagta
      121 ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc agctttactt
      181 gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa gtaccgtcaa
      241 tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta cagatccagg
      301 caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa cttttacggg
      361 ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta aagccagttt
      421 ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg gtctttgctt
      481 gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct gacttttgag
      541 ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt tttctgctta
      601 atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat ggagatccat
      661 caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga tcagttcgat
      721 cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca cacgttgacc
      781 tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta atccataagt
      841 cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa aaccgactcc
      901 taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc caactaccat
      961 atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc gataagcgtc
     1021 accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg gatagtcggg
     1081 aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt gttaaccatt
     1141 tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt ttattgagcc
     1201 gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg actttttgta
     1261 aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc ataaaaatta
     1321 ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga cgtatatcaa
     1381 ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt gtgcctaccc
     1441 cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg aatgaataag
     1501 accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca actagagagt
     1561 ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa acaatcgcag
     1621 cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct aaataaaggc
     1681 taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca aatacttgca
     1741 gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg ttccattcat
     1801 tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct tcttgagtac
     1861 caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca aatttcagat
     1921 gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga aataaaaact
     1981 gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca attcgctcta
     2041 ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct ctcatcaagt
     2101 caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg acattttcac
     2161 ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat aaatctcgca
     2221 tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat cctttagctg
     2281 atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc attattttcc
     2341 cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga tggatatgat
     2401 tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt gcttttcttt
     2461 cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca tcaaacgtcg
     2521 caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt agtctgggag
     2581 caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg ctttcttcaa
     2641 atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct aatgaaacaa
     2701 ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc gctactaaat
     2761 ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat gctactccga
     2821 ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga acaatcgcat
     2881 aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg tctacaccag
     2941 cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca gtgatgatca
     3001 actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca tacccatgct
     3061 caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt gtttctttca
     3121 ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg attttttctc
     3181 cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt atcagatacg
     3241 gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt tcctcatggt
     3301 aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca cggatcattt
     3361 ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt gcttgtctca
     3421 ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa agcagaaaga
     3481 aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga tcttttgtac
     3541 gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg ggcgattgag
     3601 ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct tgtaactccg
     3661 cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc tgttcttctt
     3721 ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc tgagtcagct
     3781 gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat acaatcagtg
     3841 caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag gaagtgaagc
     3901 caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata atcaaaacta
     3961 aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac tttttgttaa
     4021 aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat tttgatgcaa
     4081 cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg ccgacgatca
     4141 tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa tagcttccgc
     4201 ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact gtcgtaacag
     4261 ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg actgcaaatt
     4321 tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata gaggcgatat
     4381 taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt gtcaaaacca
     4441 tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg aatgtaagga
     4501 agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc ccgacttctt
     4561 ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct accttgaaag
     4621 cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct acaaatgcca
     4681 ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa cagatttgtg
     4741 cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa ttcatgcctc
     4801 ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg tcgtgttttc
     4861 aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat atctcgcact
     4921 gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag cagcttgatg
     4981 agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa atgtgctttc
     5041 atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt ttctcgtatc
     5101 gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt ttccattgat
     5161 cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt acagtggtcc
     5221 agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat gatcatgttc
     5281 aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat aaacagaaaa
     5341 ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca gcggataact
     5401 taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc cttttggccc
     5461 gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga gcccgggaag
     5521 tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt ttctcggacg
     5581 aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca accaaacttc
     5641 attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt ttgcaggaac
     5701 acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct ttaagttcct
     5761 ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg ggttcaaatt
     5821 aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa acagcagcga
     5881 acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact ttcattgtcg
     5941 ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt cgcatgattt
     6001 tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa gcgagtaatt
     6061 tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc ttcatcagca
     6121 atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc gaacacggtc
     6181 tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc ggcaaaccat
     6241 cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat taatccaccc
     6301 gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa ttgatgtgtc
     6361 ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg aacagcatgt
     6421 aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa atttaaaatg
     6481 attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt attttacctt
     6541 taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg gctgtgacaa
     6601 aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga tcacttctgt
     6661 cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt tgcttgccta
     6721 ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt tcgcaccgcg
     6781 tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac gcatcactgt
     6841 ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac cagataagat
     6901 ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat tgaatccata
     6961 gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg ttacttctaa
     7021 caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc cagaaattgg
     7081 aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct gttctttgaa
     7141 tttcactaga ttttccggtg catccggcat atccattttg tttgccacga tgatttgcgg
     7201 acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa gatagtcttc
     7261 ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta ctcgtgttct
     7321 ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc cttcaatcaa
     7381 gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca ttcctagatt
     7441 aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg aaataacgga
     7501 tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg ccaatacttt
     7561 caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa tttccggtgc
     7621 aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac cttttgccac
     7681 aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct ccgcatctcg
     7741 gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac cgtgcatccc
     7801 tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc gaaaatccat
     7861 caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac caccgtctcc
     7921 gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca ttccgtctcc
     7981 gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca taattaagtc
     8041 ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca aaagtcttac
     8101 ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt gtttaccttc
     8161 ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag tataattttt
     8221 tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa ttgaggatgc
     8281 cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa gtaggtatta
     8341 acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc tcagatgata
     8401 ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa ttattgcaat
     8461 ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac tcaatcgtag
     8521 tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc ccttgggcaa
     8581 aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct gaagaaaaag
     8641 caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca gctggtgcta
     8701 tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac aaaatcatct
     8761 cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta aataatgaat
     8821 ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca caaatgctct
     8881 tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct gacaatacta
     8941 tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag ctacaaggaa
     9001 aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact gaattggttt
     9061 cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa aaacacacag
     9121 ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat gtcattggaa
     9181 caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct ggtgatttcc
     9241 aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc caaatgattc
     9301 gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa agaagccttt
     9361 gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact cgttcaatga
     9421 acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc tgtcttgtat
     9481 gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt cgttgcacga
     9541 tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg tataaagagc
     9601 attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa aaagtagcca
     9661 gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg aagactaaac
     9721 atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca atcttcaatg
     9781 attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt tgctgctccc
     9841 atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag cactttgttt
     9901 atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc cccttcgctt
     9961 tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg aaacatcttt
    10021 gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag agctgttttt
    10081 tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc ttttgaagtg
    10141 atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc cgtttctttg
    10201 gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga aggcacaaca
    10261 gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc taaagcgacg
    10321 cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc ttttttcata
    10381 taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt ttataac
//
LOCUS       DASHRQ010000081         9723 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.085, whole genome shotgun sequence.
ACCESSION   DASHRQ010000081 DASHRQ010000000
VERSION     DASHRQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.085"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..347)
                     /locus_tag="U2M88_002426"
     CDS             complement(<1..347)
                     /locus_tag="U2M88_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEM8374768.1"
                     /translation="MINNYLHYKAINWNVIEDELDNVVWERATSLFWLDTRVPIENDR
                     SKWANLQLQEQEQLNRLLILLTNIATYQSNELGEIIRDSARSQQEIAIINNFQFTEMV
                     HTKAYNRILSIRQ"
     gene            complement(396..839)
                     /gene="nrdI"
                     /locus_tag="U2M88_002427"
     CDS             complement(396..839)
                     /gene="nrdI"
                     /locus_tag="U2M88_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEM8374769.1"
                     /translation="MQIIKVYYISLSGNTTSFLADLDTYCQKQYQKKVDYVNVKDLVK
                     LGKPLTFDINESYFVFLPTYLEGGNGIETGDVEILTTPLRQVITYNKNRDYCLGIIGS
                     GNRNFNKQFCLTAYQYADDFGFTVLDEFELKGTTEDIERIAETMQ"
     gene            986..1651
                     /locus_tag="U2M88_002428"
     CDS             986..1651
                     /locus_tag="U2M88_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEM8374770.1"
                     /translation="MKNSGCLCTVPLFASLSLDEKNEIKCLLHHQTFKKGEIIFSPSS
                     EEQLLIISLGAMKIYRISRTGKEQLIRIVKEGDYDGENYLFGLPNDCLYGEAVEETKT
                     CTLYKSDFNKVMARYPQLSYRLLQLNACKTVAVEKQLQLLAFDRIEDRLALYLNQLSI
                     AAKMGEPITLPISMRELANYLATSPETISRKLRDFQERQLITRKGKQITLLEEFWFIF
                     NYI"
     gene            1641..2096
                     /locus_tag="U2M88_002429"
     CDS             1641..2096
                     /locus_tag="U2M88_002429"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEM8374771.1"
                     /translation="MIYKEEDIKIGIVIARFNDFLTKSLLSGCEDGLTRSGVLTENID
                     VAWVPGAYEIPLTAATMAETGKYDAIITLGAVIRGATSHFDVVVNQASSGVSQVGLTH
                     KLPVIFGILTVENIEQAIERSGTKAGNLGFTYATNTIEMINLFKEIKQQ"
     gene            2246..3625
                     /locus_tag="U2M88_002430"
     CDS             2246..3625
                     /locus_tag="U2M88_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HEM8374772.1"
                     /translation="MTIQFKQTLAPTKDNSGKPIILSDATFAQRNEKVLQLMREYDFS
                     SLMIYADKEHGSNFEYLTGFIPRFEEAIQVINQDGTSTLILGNENYNKTSMARTQSEG
                     ILCPFFSLPNQPMDFTKTFADYLKDVKVDASKRIGLVDWKLLSNDMDNFHNHHSIPAF
                     IVDGIHEVFGSDKVVNATQLYMHPKEGARVTNNAEEIARYEYGASLASDAVLEAMNQL
                     TVGQSELAAGNHLNRDGQYPSVVTIAAFGDRFEGANIYPTDNTLNDGDKVALTVAYKG
                     GLSSRSGYAVKEESQLAEHDPGYLEEVVYPYFQAYKWWLDNLTIGRKGGDFYQAFSDF
                     YPQATYGWNLCPGHLVADEEWLSSPFYQDSDATIQSGYIFQLDFIPSQAGHNGVSAES
                     TIAVADAALREQIKAEQPELWARICNRKDYLKEALGLELSEHLLPLASTFAYYRPFLL
                     DKKTAIYFG"
     gene            3762..4604
                     /locus_tag="U2M88_002431"
     CDS             3762..4604
                     /locus_tag="U2M88_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEM8374773.1"
                     /translation="MPLDEQIELLFTIDEGYLNPLKVALTSIRQNNPGQAFRIWLIHE
                     SIAMHTIRELQKLTDYLQFGFEAIKIDGSRWNSAKTEDRYPKEMYFRLLAGEILPKEM
                     KRVIYLDPDILVVNPLLDLWQTDLEGYMLAAATHVGLTDVSTRVNQMRLDVDHAYYNS
                     GVMVIDLDKAREIIKWSDIAQMIEKYNLLLILPDQDILNRLYGKYTKEIPEEIWNYDT
                     RKYMRYFTKSLAQHDIHWVMAHTAILHFCGGPKPWDDKHDNRFTSLYLTYQNQLKRIE
                     DLMK"
     gene            4678..6126
                     /locus_tag="U2M88_002432"
                     /pseudo
     CDS             4678..6126
                     /locus_tag="U2M88_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            6241..8013
                     /locus_tag="U2M88_002433"
     CDS             6241..8013
                     /locus_tag="U2M88_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM8374774.1"
                     /translation="MNNNETKKTYELRITADEIKSTDPTFTMKNLSLGDNYQPKDAYF
                     RTAEGEITRRNYGKNERKISNQTLPRLAIQMFEQSIVSLPESERATFPVCRYHSGDEI
                     IRGIFPDVDSFKKKRKSIEFLIYKYNEDKQIVIYCWSIFSTLLFVQECLKRFGTSGDE
                     FILVYKGKEKKEIRVGETQTMTNHLLKSYVLHLLKSKNLILRGAPGTGKTFLANEMAA
                     DVVSNGRTTQINELSEDEKSRIGFVQFHPSYDYTDFVEGLRPTTSDDGVVSFTLKDGS
                     FKSFVEKAKKTKKVNGQDNFEEAWAKFFDAVTEASIEGEGYNKLKTLTGKPVRNLLAY
                     ERNEIQGVYPAKTNTYLNHDQIYNVYRGLPGTPKGGFDAYRKAIVKHLKEEFGLKNYT
                     ASTVDSTNNQNYVFIIDEINRGEISKIFGELFFSIDPGYRGHKEGVYTQYANLHENPE
                     EKFYIPNNVYIIGTMNDIDRSVDTFDFAMRRRFTFLEIKAEDSAQNMLTQEKTKELMT
                     RLNEAIVAKEKGGLSTDYQIGASYFLSIDNGEETMEQLWDTKLYPLLKDYFHGEHKAK
                     EKLEILEKVYSNDGDLDEAETSTR"
     gene            7988..9280
                     /locus_tag="U2M88_002434"
     CDS             7988..9280
                     /locus_tag="U2M88_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine 5'-monophosphate oxidoreductase"
                     /protein_id="HEM8374775.1"
                     /translation="MKLKPLHDNQEINDSVVQHPIITNLVNRDLSSLEKENFIIFPQQ
                     LNQSKDLSEDNLIFQSRNGKIWTCNVVGILSDSTDELRIHSRFANQMKHEDFFLRYML
                     QRVLNYNVINDHFSASKKMTYYDLLVFLFPYYLNEAMRKGTYKEYVKREYNNANIKGA
                     VDVAKHIRSNVPFVGKVAYRTREFSYNNHLTQLIRHTIEKIQNEYDFLLSGDEDTKEN
                     VVLIKQTTPGYARLNQFNILQENILHPVKHSYYEEYSALQQICIQILSEEKSGFGSDK
                     NQIHGIIIDVSWLWEEYIGKVTGWKHYGRDKGLATMHLFQEPNRSPRYPDFTFNNIPI
                     DTKYKKHLDTRNDYNQLVTYIHIMNLDQADTMKGGFLQPTSDPLPANHGYKKIGILSG
                     LGGEIFTYHFFIPQAASSYLEFVEQMREVEAALQTTFQ"
BASE COUNT         3407 a         1576 c         1840 g         2900 t
ORIGIN      
        1 aattggcgta tgcttaaaat gcggttatag gcttttgtat gaaccatttc agtaaattga
       61 aaattattta tgattgcaat ctcttgttgg gaacgtgcac tatctcgaat aatttctcct
      121 aattcattgg attgataggt agcgatatta gtcaaaagaa tcaacaagcg attgagctgc
      181 tcttgttctt gtaattgtaa atttgcccat tttgatcgat cattttcgat ggggacacgt
      241 gtatccaacc aaaaaagtga ggttgctctt tcccatacaa cgttgtctaa ttcatcctca
      301 atgacattcc agttaattgc tttatagtgt aaataattat ttatcatagt acctcctaaa
      361 atattgtttg aaaggggatg atatggtgtc attttttatt gcattgtttc tgcaattctc
      421 tctatatctt ctgtcgtacc ttttagttca aattcatcca acacggtaaa accaaaatca
      481 tcggcgtatt gataggctgt taggcaaaat tgtttattga agtttctgtt gccactacca
      541 ataattccta aacaatagtc tcggttttta ttgtaagtaa tcacttggcg caatggtgtt
      601 gtcaaaattt cgacatctcc tgtttcaata ccatttccac cttcaagata agtaggcaga
      661 aatacaaaat aggattcatt gatgtcaaaa gtgagtggtt ttcctaattt gactaaatct
      721 tttacattca cataatctac cttcttttga tattgttttt gacaatacgt atctaaatct
      781 gcgagaaaac tcgtggtatt tccacttaaa ctaatataat agactttaat tatttgcatg
      841 tatcacacct ctttttaata aataaccaca tatatagtgc ttgttttaat tagaaacgct
      901 atatatagtg tttattgtac atgacatatc gttatagaaa attgacgatg gtcaattatt
      961 ttgattttag aggagagaaa attacatgaa aaattcaggt tgtttatgca ccgttccact
     1021 cttcgcctct ctttcattag atgaaaaaaa tgagataaag tgtttgcttc accatcagac
     1081 gtttaaaaaa ggagaaatta ttttttcacc aagtagtgaa gaacaactgt tgattatatc
     1141 tttaggggct atgaaaatat atcgaattag tagaactggg aaggagcaat taattcgtat
     1201 cgtaaaagaa ggggattacg acggtgagaa ctatctcttt ggtctaccca atgactgcct
     1261 atatggtgaa gctgttgaag aaaccaaaac ttgcactctc tataaatccg actttaataa
     1321 agtgatggca cgctatccac agctcagtta tcgattgtta caattaaatg cttgcaaaac
     1381 agtagctgtg gaaaaacagt tgcagcttct agcgtttgat cgtatagagg accggttagc
     1441 cttatatctt aaccaactat ctatagcagc aaaaatgggg gagcctatta ctttacctat
     1501 ctctatgaga gagttggcaa actatctagc taccagtcct gaaactattt ctcgtaaatt
     1561 aagagatttt caagaaagac aattgattac tagaaaagga aagcagatta cactattaga
     1621 agaattttgg tttatattta attatatata aagaagaaga tattaaaatt gggattgtca
     1681 ttgcacgatt caatgatttt ttaactaaaa gcttactaag tggttgtgaa gatggcctta
     1741 cgcgttcagg cgttttgaca gaaaatatcg atgttgcttg ggtgccgggg gcctatgaaa
     1801 tcccacttac agccgcaacg atggctgaaa caggtaaata tgatgccatt attaccctag
     1861 gtgcggtaat cagaggagct acaagtcact ttgatgtggt ggttaaccaa gcgtcaagtg
     1921 gggtatctca agtaggctta acccataaat taccggtcat ctttggtatc ttaaccgttg
     1981 aaaacatcga acaagccatt gaacgctctg gtactaaagc tggtaacctt gggtttactt
     2041 atgcaacgaa tactattgaa atgattaatt tattcaaaga aattaaacaa caataagtca
     2101 tcattctata tgaaaatatc tgtaacagca tagggctagc tttaattttc aatcatggta
     2161 agatggaaga taggaaaata aagcgcattc aattaattat caagcctttc tgatgatttg
     2221 cgcaaaaagc ttaaggagac ttcatatgac gattcaattt aaacaaacat tagcgccaac
     2281 caaggataat tctggaaaac caatcatttt aagtgacgct accttcgctc aacgtaatga
     2341 gaaagtcctt cagttaatgc gtgaatatga cttctcatca ttgatgattt atgcagataa
     2401 agaacacggt agtaactttg aatatttaac tggttttatt ccacggtttg aagaagctat
     2461 ccaagtaatc aaccaagacg gaacgtcgac actgatttta ggtaatgaga attacaataa
     2521 aaccagtatg gcacgtacac aaagtgaagg tattctttgc ccattcttct ctttaccaaa
     2581 ccaacctatg gatttcacta aaacttttgc tgactatctt aaagacgtta aagtagatgc
     2641 ttcaaaacga attggtttag tagactggaa gcttttatct aatgatatgg acaacttcca
     2701 caaccatcat tcaataccag catttattgt agatggtata catgaagttt tcggttctga
     2761 caaggtagtg aatgcaaccc aactatacat gcatccaaaa gaaggtgctc gtgtgacaaa
     2821 caacgcagag gaaatcgctc gttatgaata tggtgcttcc cttgcttctg atgctgttct
     2881 tgaagcaatg aaccagctaa ccgttggcca aagtgagtta gcagcaggta atcacttaaa
     2941 tcgcgatggt caatatccat ctgttgtgac cattgcagcc tttggtgatc gctttgaggg
     3001 tgctaatatt tatccaactg ataatacgct aaatgatggc gataaagttg cattaactgt
     3061 agcctataaa ggaggcttgt ctagccgctc tggctatgca gtaaaagagg aaagtcagct
     3121 cgctgaacat gatccaggat acttagaaga agtggtttac ccatatttcc aagcctataa
     3181 atggtggcta gataatttaa ccattgggag aaagggtggc gatttctacc aagcattcag
     3241 tgacttctat ccgcaagcta cttatggctg gaatttgtgc ccagggcatt tagtagctga
     3301 cgaagaatgg ttaagctcac cattttatca agactctgat gcaacaattc aaagtggcta
     3361 catcttccaa ctagacttta ttccttcaca agcaggccac aatggtgttt cagctgaatc
     3421 aacgattgcg gttgcggacg cggcactacg tgaacagatt aaggctgaac aacctgaatt
     3481 atgggcacgt atctgtaacc gaaaggatta tctaaaagaa gcattaggtt tagaattatc
     3541 agaacattta ctacccttag cgagtacctt tgcctactat agacctttct tattagacaa
     3601 aaaaactgct atctactttg gataaaaaaa tgcccctcaa gattgtttgc caaacttgag
     3661 ggttttttgt ctatttttaa gtgaataatg acggactatc caaacggaat cgccataaag
     3721 atgttaatat atgtataata ttataaagaa attggaggac aatgcccttg gacgagcaga
     3781 ttgaattatt atttaccata gatgaaggct atttgaatcc cttaaaagtg gctttaacat
     3841 cgattcgtca gaataatcca ggccaagctt tccgcatttg gttgattcac gaatccattg
     3901 caatgcatac gattcgcgaa ttgcagaagc taacggatta tctacagttt ggttttgaag
     3961 cgattaaaat tgatggtagt cgatggaata gcgctaaaac tgaagataga tacccaaaag
     4021 agatgtattt ccgcctgctt gcgggtgaaa tattacctaa agaaatgaaa cgcgtgatct
     4081 atttagaccc agatatttta gtggttaacc ctttattaga tttgtggcaa acagatttag
     4141 aaggttatat gctagcagct gctacacatg ttggattgac ggacgtttct acacgagtaa
     4201 accaaatgcg acttgacgtt gaccatgctt attacaattc tggtgtgatg gtgattgatt
     4261 tagataaagc tagagaaatt attaaatggt cagatattgc acaaatgatt gaaaaatata
     4321 acttgctatt aatcctaccc gaccaggata tcctcaaccg attatatggt aaatacacta
     4381 aggaaattcc agaagaaatt tggaattatg atacgcgtaa atatatgcgg tatttcacca
     4441 aaagtctcgc tcaacatgat attcactggg tgatggcgca tactgctatt ctccatttct
     4501 gtggtggccc aaaaccatgg gacgataaac atgataatcg ttttaccagt ctttatctta
     4561 cctatcaaaa tcaattaaag cgtattgaag atttgatgaa gtaaagtgaa acaagtgttt
     4621 ttcattacct acggtggttt aatgcttatt tattatcgtt aaatcatgct tatacacttt
     4681 acaaaatttg attttcgaaa tggccgtcca acgaagtctg cgatcttaac cacgagttcc
     4741 attgacatgg ccaaaaagta ttatcgagcg atcaaagaaa tgacgaaaga acctgactgg
     4801 ttaacaaaag agtttcctaa tcagcccatt cgtgaaggaa gaacgatgga ggatcctgat
     4861 tttccacgaa tcgctattac ctattcatta gaagaaaata accaagattc aagtgcccaa
     4921 caagaagaaa tgaaaaaaat tattgaggaa tataatcagt attttgatac tgcctggtca
     4981 ttagcagaca ttgagcgtta taacggagac atcaataatt gtttggcacg aaaaagagcg
     5041 gaattcaaac aatttgggaa acaaatcgat ttagtaatcg tagtggatcg tctattgaca
     5101 ggttttgacg cacctacgat tcaaacccta tttgtggatc gtaatctaga gtatgcagga
     5161 ttgattcaag ctttttcacg tacgaaccgt acgtatcctg aaaagacaaa aggattgatt
     5221 gtgacgtttc gtaagccagc gacaatggag aagaatgtcg aggatgcgac aaaactgtat
     5281 tcagaagcaa aagaagaatc gggtttgatt tatccaagtt atcaggaatc caaaaaaaga
     5341 ttcaagcaag cgtatggtaa gttaaaggag gtttcatcca tgaaatccat tgatgaacat
     5401 acgccacttg aaatccgtat cgagtacgtt aaagcatttc aagaattgaa tcgtgcgtat
     5461 gaatcgttag tgacttatga cgattacaat gatgacatgg tgcagtcttc aactttgaac
     5521 aatcaaattc aacttttgga agaacaaatt ggcgtgtacg agaccgtgaa ggggtcatta
     5581 atagaggaag aaccagaaaa agagggagaa gatttttcaa cgatcgaatt ttatagtgaa
     5641 aataccacta agttgtatga tattgattct acttatattg atcaattact aggaacttat
     5701 gctgcgaata gttcagatgc acgagatgaa attgaaaaag ctttggctaa attgaataaa
     5761 acagaaggag tcaaagaagt ttaccgacaa attttaaatg catttgatgt ggggacttta
     5821 gatagcaatg aagatatttt tgtcattaaa cgtcgctatt ttacacaagc aagtgatgaa
     5881 ttgattcatg ccttttccaa tgagtggttt gtttctgaga aagaattaca tgcttctaat
     5941 ctgcaatata tgccaggtga agatccaatt cctaatatga aagcaattat caacagtaag
     6001 gattacgaag gatataaagc caaacatcca aaagcgaagc catttaaata cccacaagaa
     6061 atgaagcgag cctggcgtaa aatgttagat gatgagttga ttccacttga gaatgaattg
     6121 aggtaaacga gaaaattgaa gaaattgtat gataaacatg ttaataaatg atgctcgaaa
     6181 taaaatttgg ttttattttt acattgatac ggtaaataaa atttgaaagt agaagaaaat
     6241 atgaacaaca atgaaacaaa aaaaacatat gaactaagaa taactgctga tgagattaaa
     6301 tcaacagacc caacatttac tatgaagaat ctaagcttag gggataatta tcaaccaaaa
     6361 gatgcttact tccgtacggc agaaggcgaa ataactagaa gaaactacgg taaaaatgaa
     6421 cggaaaattt caaatcaaac attaccgcga ttagctattc aaatgtttga acaatcgatt
     6481 gtttctctac cagaaagcga aagagcaaca tttcctgtgt gtcgatatca ctcaggagat
     6541 gagataatac gagggatttt tccagatgtt gacagtttca aaaaaaaaag gaagtccatt
     6601 gaatttttga tttataaata taatgaagac aagcagatag tcatctattg ttggagtatt
     6661 ttttcaactt tattatttgt acaagaatgt ttgaaacgtt ttggaacatc tggggatgaa
     6721 tttattcttg tttataaagg gaaagagaaa aaggaaatac gagtaggaga gactcagact
     6781 atgaccaatc atttgctgaa atcatatgtt ttacatttac tcaaatctaa aaaccttatt
     6841 ctacgtggcg ctcctggaac agggaaaaca ttccttgcaa atgaaatggc agctgatgtc
     6901 gtttcaaatg gcagaactac acaaatcaat gaattgagcg aagatgaaaa gagccgaatt
     6961 ggatttgtcc agtttcatcc tagctatgat tatactgatt ttgtcgaagg tttacgtcca
     7021 actacatccg atgatggcgt tgttagtttc accttgaaag atggaagctt taagagcttt
     7081 gttgaaaaag caaagaaaac aaagaaagta aatggacaag ataattttga agaagcttgg
     7141 gctaaatttt tcgatgcggt aacggaagct agtatagagg gagaaggcta caataaactt
     7201 aaaacattga caggtaaacc agtacgcaat cttctagcct atgaaagaaa tgaaattcaa
     7261 ggtgtttatc ctgctaaaac caatacttat ctaaaccatg accaaattta taatgtctat
     7321 cgtggattgc caggtacgcc aaaaggtgga tttgatgctt atcgaaaagc gattgtaaaa
     7381 catttaaaag aagaatttgg cttaaaaaac tacacggcgt caacggtaga ttctacgaat
     7441 aaccaaaact atgtgtttat cattgatgaa attaatcggg gcgaaatttc caaaattttt
     7501 ggcgaattat ttttctcaat tgatccagga tatcgtgggc ataaagaagg tgtttacact
     7561 cagtatgcaa acctccatga aaatccagaa gaaaaattct atattcctaa taatgtttat
     7621 attattggga cgatgaacga cattgaccgt tctgttgaca cttttgattt tgctatgcgc
     7681 cggcgattta ctttcttaga aataaaagca gaagattcag cacaaaatat gctgactcaa
     7741 gagaaaacaa aagaacttat gacgcgttta aatgaggcga tcgttgccaa agaaaaaggt
     7801 ggattgtcaa ctgattacca aattggtgct agttactttt taagtatcga taatggtgag
     7861 gagacaatgg aacaattatg ggatactaag ttatatccat tattgaaaga ctattttcat
     7921 ggtgaacata aggcgaaaga aaaattagaa atactagaaa aggtatactc taatgacggt
     7981 gatttagatg aagctgaaac ctctacacga taatcaagaa atcaatgact cagttgtgca
     8041 acatccaatc attacgaact tagttaatcg tgacttaagt agtttagaga aagaaaattt
     8101 tattatcttt ccgcaacagt taaatcaatc caaggatcta agtgaagata acttgatatt
     8161 tcaatcaaga aatggaaaaa tttggacttg taatgtcgta ggtatcctta gcgattcaac
     8221 agatgaatta agaattcatt ctagattcgc taatcaaatg aagcatgaag atttcttttt
     8281 acgttatatg cttcagcgtg ttttgaatta caacgtgatt aatgatcatt ttagtgcatc
     8341 taaaaagatg acatactacg atttgttggt atttctcttt ccttattatc tgaatgaagc
     8401 aatgcgtaaa gggacataca aagaatatgt caaacgagaa tacaataatg ccaatatcaa
     8461 gggagctgtt gatgttgcaa aacacatccg ttcgaatgtt cctttcgttg gaaaagttgc
     8521 ttatcgaaca cgagagttta gctataataa tcacctaacc cagcttattc gtcatactat
     8581 tgaaaaaatc caaaatgagt acgatttctt acttagtgga gatgaggata cgaaagaaaa
     8641 cgtggttcta atcaaacaaa caacacctgg ttatgctaga ttaaatcaat ttaatatcct
     8701 tcaagagaat atccttcatc cagtcaaaca tagttattac gaggagtatt cggctcttca
     8761 acaaatctgt atccaaattt tatcggaaga aaaatcagga tttggaagtg ataaaaatca
     8821 aatacatgga atcattatcg atgtgtcttg gctatgggaa gaatacattg gaaaagtcac
     8881 tggttggaaa cattatggca gagataaagg gttagcaaca atgcatttat ttcaagagcc
     8941 caaccggtcc ccacgatatc cagattttac atttaataat ataccaattg atacaaaata
     9001 caaaaaacat cttgatacta gaaatgacta taatcagtta gtcacatata ttcatataat
     9061 gaatttagac caagcagata caatgaaagg cggctttctg caacctactt ccgatcccct
     9121 tcctgcaaat catgggtata aaaaaatagg catattaagc gggctaggag gagaaatatt
     9181 cacatatcat tttttcattc cgcaagctgc gtcaagttat ttggaatttg tagaacagat
     9241 gagagaggta gaagcagcac tacaaactac ttttcaataa gtaggaatta tagaaatttt
     9301 actcaaaaaa tcttcattaa aaaatctgac taaataagtt gagaaaacag aatatacttc
     9361 aatatccagt catttataaa tatcattcac tcatgatacc cagctccgta tcacaaataa
     9421 tgatattttt taagagaaaa aattatccct attttaaata atagcaatag gtagtggtag
     9481 agagaaattt ataaataact ttgttatttg ctttggtagc caagtaagtt gcaaaagaaa
     9541 aatagatact gttaaaaaga taatgtagac atagaaacaa atacgtaaaa ttttagttag
     9601 aatgatggca aggagaaaaa acatgataga tgaaaaaaag tgtttaaaaa atctattgga
     9661 agatatggat gatattgaag aaaaactatc taaatggact gggaaaacaa atatatttca
     9721 gaa
//
LOCUS       DASHRQ010000082         9699 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.086, whole genome shotgun sequence.
ACCESSION   DASHRQ010000082 DASHRQ010000000
VERSION     DASHRQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.086"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1023
                     /locus_tag="U2M88_002435"
     CDS             <1..1023
                     /locus_tag="U2M88_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEM8374776.1"
                     /translation="ELKPKHERQNFLVGDDRLDHAVAFLWKDPQTGETVGASYQGTIV
                     DFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKFFESSIDLLSYAALNREKLQDAW
                     LVSMDGLKHHVISHYVEESISELRRKQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDP
                     FTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVS
                     AHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKVCERADGTYNFDRFYSRKANIKD
                     VNAGILLSYKAEQYYKGYKKHEHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1034..3043
                     /gene="topB"
                     /locus_tag="U2M88_002436"
     CDS             1034..3043
                     /gene="topB"
                     /locus_tag="U2M88_002436"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEM8374777.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            3159..3308
                     /locus_tag="U2M88_002437"
     CDS             3159..3308
                     /locus_tag="U2M88_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374778.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            3389..3985
                     /locus_tag="U2M88_002438"
     CDS             3389..3985
                     /locus_tag="U2M88_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEM8374779.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNTIITYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            3998..4897
                     /locus_tag="U2M88_002439"
     CDS             3998..4897
                     /locus_tag="U2M88_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEM8374780.1"
                     /translation="MTPTQLKAKVKNISREKEIDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            4900..5031
                     /gene="ssb"
                     /locus_tag="U2M88_002440"
     CDS             4900..5031
                     /gene="ssb"
                     /locus_tag="U2M88_002440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEM8374781.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            5053..5385
                     /gene="ssb"
                     /locus_tag="U2M88_002441"
     CDS             5053..5385
                     /gene="ssb"
                     /locus_tag="U2M88_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEM8374782.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            5430..5663
                     /locus_tag="U2M88_002442"
     CDS             5430..5663
                     /locus_tag="U2M88_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374783.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(5822..5944)
                     /locus_tag="U2M88_002443"
     CDS             complement(5822..5944)
                     /locus_tag="U2M88_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEM8374784.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            6134..6358
                     /locus_tag="U2M88_002444"
     CDS             6134..6358
                     /locus_tag="U2M88_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374785.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            6801..7007
                     /locus_tag="U2M88_002445"
     CDS             6801..7007
                     /locus_tag="U2M88_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEM8374786.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            7007..7258
                     /locus_tag="U2M88_002446"
     CDS             7007..7258
                     /locus_tag="U2M88_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374787.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            7274..7429
                     /locus_tag="U2M88_002447"
     CDS             7274..7429
                     /locus_tag="U2M88_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374788.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIE"
     gene            7439..7711
                     /locus_tag="U2M88_002448"
     CDS             7439..7711
                     /locus_tag="U2M88_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374789.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMEKEDNENE"
     gene            8156..8335
                     /locus_tag="U2M88_002449"
     CDS             8156..8335
                     /locus_tag="U2M88_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374790.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            8394..8750
                     /locus_tag="U2M88_002450"
     CDS             8394..8750
                     /locus_tag="U2M88_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374791.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         3486 a         1612 c         1994 g         2607 t
ORIGIN      
        1 gaactaaagc cgaaacatga acggcaaaat tttttagttg gagacgaccg tttagatcat
       61 gcggttgctt ttttatggaa agatccacag acaggagaga cagtaggggc aagttatcaa
      121 ggaacgatcg tcgattttaa tcgtttcggt aaacggggaa cgtataaaca cattgacaag
      181 aatcctacgc caaatcacgg atttaattta aaaataggcg atccgaagca tttaaaattc
      241 tttgaaagta gtattgatct gttaagttac gctgcattga atcgggagaa actacaagat
      301 gcctggttag tttcgatgga cgggttaaaa catcatgtaa tcagtcatta cgttgaagag
      361 tcgatttctg aattaagacg aaaacaaaca tttcctcaat cgattgaaat atgtgtggac
      421 aatgatcgtg caggacatat tttttatgaa aaagaacaga tgaagggaat cgttgatcct
      481 tttaccaata agaaaatccg ttgtgagcga ggaattccaa atgattggca agtacctaaa
      541 gaatacaaag ctacttatga agcagtcgca aaagaaatga gtgttgaacc tgaagcaatt
      601 atggccatac acaaaactga aactaattta cagctgacaa atcaattggt gtctgctcat
      661 gatgttcaat ctacgtttgg aaagatgttg gcgaagggcg aaccagtaga aacaattgat
      721 ttgaaagaag catgtaccac ggtcgcgaag gaactgaaag tatgtgaacg cgcagatgga
      781 acatacaact ttgatcgttt ttatagtaga aaagccaaca taaaagacgt taatgcagga
      841 atccttcttt cctataaagc agaacaatac tacaaaggct ataaaaagca tgaacatgaa
      901 tttgtacctg aagtgaaaaa ggattggaat gatcaattga agcatgagat ccagcaacaa
      961 gaaatcagaa agcaaaaacg tgcgatgttg tttcaacaag gcagacaaca agaaagggag
     1021 tgaagtcgtc actatgaaaa ccgtcatttt agcagaaaaa ccgtcacaag cgaaggctta
     1081 tgccgatagc ttttctaaag ctacccgaaa agatgggtat tttgagatcc aagatcgatt
     1141 gtttacagga gaaactgtga tcacgtatgg ttttggtcat ttagtcgagt tagattcgcc
     1201 cgatatgtac gatgaaaaat ggaaacaatg ggcattagaa catttgccta ttttccccaa
     1261 tcaataccac tatcatgttc ctaaagataa gaaaaagcag tttagtgtcg tcaaacgaca
     1321 gctgcagtca gctgatacga ttgtgatcgc gactgatagc gatcgtgaag gggagctgat
     1381 tgcctggtca atcattcaac aagcaggcgc caatcaagga aaaaccttta aaagactttg
     1441 gatcaactcc ttagaaaaag aagccattta tcagggattc caacagttac gagacgcaga
     1501 agagacctat ccaaaatttg aagaagtaca agcacgtcag atcgccgatt ggttaattgg
     1561 gatgaacggt agcccactgt atagtttact cttgcagcaa aaaggcatac caggaagttt
     1621 ttctttaggt agagtccaaa ccccaacact ctatatgatt tatcaattgc aggaaaagat
     1681 tagaaacttc caaaaagaac catattttga aggaaaagcc caagtaatcg ctcaaaatgg
     1741 cgcatttgat gcgaagcttg atccaaatga aacccaagcg acacaagaag catttgaagc
     1801 ctatttaaaa gaaaaaggtg ttcaattagg aaaacagcca ggtacgatcc ttcaagtcga
     1861 aacagagaaa aaaagtgcgg ctagcccacg tcttttttct ttatcgagcc ttcagtctaa
     1921 aatgaatcaa ctcatgaaag ctagtgccaa agatactttg gaagccatgc aagggttgta
     1981 tgaaggaaaa tatctaagct atccgcgaac ggatacccct tatattacgg aaggggaata
     2041 tgcgtatctg ttggatcact tagacgaata caagcatttt ttaaaagccg aagcgattcc
     2101 gactcctatt catacaccga atagtcggta tgtgaataat aagaaagtcc aggaacatta
     2161 cgcgatcatt cctacaaaaa cggttatgac agcagcggct tttgaacaac taagtccact
     2221 gcagcaggct atttatgaac aagtgttaaa aacaacggtg gccatgtttg cggaaaagta
     2281 tacctatgaa gaaacaacga tcttgacgca agttcagcag cttcagttaa aggcaatcga
     2341 aaaggtgccg ctagatctag gttggaagaa gttatttgga aaagagagcg acggaaaaga
     2401 gaaggaagaa gagccgctac ttcctaaagt gacaaaagga gaaatggtga cggtggatct
     2461 acaagttctt gaaaaagaaa ccaagccacc acaaccttat accgaaggta cgttgatcac
     2521 tgcaatgaaa acagctggga aaaccgtgga tagtgaagaa gcgcaatcca tattgaaaga
     2581 agtggaagga atcggtacag aagcgacacg ggcgaatatt attgaaacct tgaaacaaaa
     2641 ggaatatatc aaggtagaga aaaataagct cgtggtgacg aataaaggga ttttgctttg
     2701 ccaagccgtg gaaaaagagc cgctgcttac gagcgccgaa atgaccgcca aatgggaaag
     2761 ctacttgtta aagatcggtg aacgaaaagg aacacaaacc acttttctag ccaatatcca
     2821 aaaatttgtc tctcacttat tggaagtcgt accagggcaa atccaatcca ctgattttgg
     2881 atcgacgtta caagaagtga aagcagccag tgaaaagcaa gaagccgcgc gtcatttagg
     2941 aatttgtcca aaatgccagg aacaagaagt cttactctat caaaaggtgg cagcttgtac
     3001 gtctgaagcc tgtgatttta aaaggtcaga agggttcatt tgaagcaacg attgttttaa
     3061 aagaagactt cactacaggc tttgaatttt ctgaaaagaa aaaaacgaac ttcaagaaaa
     3121 gaacacgccg cactgcaaaa taatggaggg ttaatcttat gcgtaatacc acacacaaac
     3181 aacaagaaat cgaaaatcaa aaagccaaaa ttcgttcaga acaggaaaaa atgcgtgcca
     3241 gcaaacgaag actgcagcaa ttgaagaacc agcagaggat tcaaaaagaa atcgaattag
     3301 atcgataaaa tcttcctaac tattgtatac gtataaactt atgttatact gtttttataa
     3361 gtttatacgt atacaaagga gggagaaaat gacaaaagca atggtataca agctgctaga
     3421 agaatttaat ggaactttgg ctttgaagga tgccaaaaaa gcgggcattt ctcctgtaac
     3481 gatcaaacgt atggtagatc gaggggagct ggatcgagag tatccaggtt ttttcacctt
     3541 acctggtcaa tttcctgatg aattgtttat ggcgcaaaaa aaatatgaac gcggcattat
     3601 ttcacatatt accgcgttag atttgtatga tttaacagat atgatcccta gacagatcga
     3661 tctgactgta ccttacggtt atcacgtttc tgaaaaagga ttgaaggaat tcgcagttga
     3721 gctacactat acaaaagcgg aatggtatga attaggtaag attgaaataa aaagtcgcta
     3781 tgggaatact attataactt atgatccaga aaaaaccttg tgtgacattt ggaatccctg
     3841 gtataacgtt gaggatgaaa tcaaagtaaa agcaatcaaa aactacatgg agtcggacag
     3901 aaaaaatctt agaaaattga atgagtatcg acgaattttg cctacagata aaacgatgcg
     3961 ctcttatatt atggctttaa attaaaggag aatacacatg acacctacgc aattaaaagc
     4021 aaaagtgaag aatatttcta gagaaaaaga aattgatccg cagcttgttt taagacattt
     4081 tatgatggaa aaattcttgg aaaaaatctc agagtctcct tatcgtgaaa attttgtgtt
     4141 aaaaggcggc ttcttaattg gctcaaagta tggcattgaa aatcgaacaa ctaaagacat
     4201 tgatacgact ttacgggaaa tgaaagtaac caaagaaaca ttgacaacgg ttctaaacga
     4261 tattttttca acgccgacga aagaaggcat tcaatttgag attcaaggaa tgaaagaaac
     4321 gagggaagct gattattatc cagggtttag cttacgtgta ctggcacatt tggaaaacat
     4381 gcggccagat tttaaggtag atgttactac aggtgactcg atctatcctg cgacgatcac
     4441 tcacagtcat aaattgatgt ttgaggatcg aacgattcct ttggaatcct atcctacaga
     4501 acaaattatt gctgaaaaat taagtgcaac ctttgatttt ttaacagata attcgagagg
     4561 aaaagatttt tatgatctct atactattcc taaaatggag aagatcgatg agaagacctt
     4621 gaaagatagt gtgagaaata catttacacg tcgaggaaaa actgatccgt taaagatata
     4681 ctatgaaaag gatatggatg tactgaaaaa tagtcacgag ctaaaagata cctggaagaa
     4741 atatcaaaaa agcaatcctt atgctgcgtc gatcacgtac gatgagacaa tggattcgat
     4801 tagtaatttg atggaaaaag tgattcacgt cgaagaaaaa gagcgaaaac tagcttatca
     4861 aagacaccaa cagatgaata gagaaatgga gagataaaga tgattaataa tgtgacatta
     4921 gtaggacgct taaccaaaga tcctgattta cgttataccg caagtggtac tgcagtagcg
     4981 acttttaccc tagccgtcaa ccgaaatttc acgaatcaaa acggcaattg agaagccgat
     5041 tttattaact gcgtgatttg gcgcaaacca gcagaaacaa tggcgacatt agctaaaaaa
     5101 ggtagcttga ttggtgtagt tgggcggatt caaacgcgga cttacgacaa ccagcaagga
     5161 caacgtgtat atgtgactga agttgtagca gataactttc aactattaga aagtaaagca
     5221 gcgacagaga gccgtgcgca cgctgatcaa tcgtccacca gtccaagcac cgccaccttt
     5281 gaacaacgga atacagcgac gcctaacaac aatggattga acgcttcaca gaatccattt
     5341 ggtggacaat caatcgatat tagcgatgat gatctaccgt tttagtttga gaaagaagga
     5401 gtaagcatgg caaaaaaatt agtctgacga tggatctatt gatacccgta attgcattta
     5461 tagacgggat ttctttgggc gtcactgaaa atcctttggt aaatagaatt gcgaaaggat
     5521 cactatttct tgtattcgtg tatcttgtct gttcttatct tttaaaaaga gcaggaaagg
     5581 ataattcctt ttggcgattg actaaagtca cagtagcagc gacagctgtt atacttacgg
     5641 ctattctacc gctttatatg tagagttaac gggaaatcgt tgaacttctt gcgaaatatt
     5701 gctataatga acatgaaaaa gaggagttgc gctaacaacc cctcattaac agcaccgatt
     5761 aaagaacggt ggcagaatat tagttatttg gaaataaccg ctgacctgct aaagtagacg
     5821 gttatttctt tttgtcattt ttaagcatat ttacaatcaa actaatcaaa gcaatggtaa
     5881 acataccaaa accaagaatg gtttgaattg tctcatatgc ggacaaaagg cttactcctt
     5941 tcatagattt tagcacttac attcataagc accaccacct ttccagaagt cgccaccgtc
     6001 ttcaaacttt actgtcttga ctattatagc atatctaaaa tcgaataaaa attctcaatt
     6061 ttagctgttt ttgctataat tttagcgagg tgatcgtgat ggaacaatat attggtttac
     6121 tcgataaatt agggtgatca agacacagcc attgcttgtg cgatttacat tacgcacgag
     6181 tcatgaacca atcaattgtg tagtggccaa tatagaaata gcggataaat tgctgatttt
     6241 ggaagaaggc aagtatacta tttcagtgac aggtcacttc aataaacgaa agcaactagt
     6301 cattgaatca atgcaaatta gaaaccctga tcattttacg cgatcgatga gcatataaaa
     6361 gaagagctgc agtcctaaat gaggattgta gctctttttg ttttacatag aaacagtaaa
     6421 aatgaaaagg cctgtttagg aaggcacaag cgccttctca attgaaagac ctttctctca
     6481 ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac gctacgcgtt cactaagata
     6541 aagggtcttg catagcaaga caaaatagaa aaaatttcct ctcaccaaaa ggttcgctcc
     6601 tgttcgttat ctctttattg atttgtcaga ccggcaacaa gttgcctttt atcaccaaaa
     6661 agcgacaagg acaagggtaa atggcacgct gcgcgccctt gtcttgtcgc ttttcgttga
     6721 ttcattctgg ccaacaaatc aataaagaaa gaactaacga aaggaagtaa aacctttcct
     6781 aagaggaggc agaatcaatc atgcgaaaga aatttgatta ttggggtgtt cccttcagcg
     6841 aattattccc aaactatcat gcaccacata ctgtcgaatg cgattgtgga gaaagagcga
     6901 aatgtatcaa aagctattgt ttgtatcaat gtccaacatg cgggaaaaaa tataccttgt
     6961 cttatggcga ttatatttta attgatgaga aaggaaagaa acgctgatga aaacacaaca
     7021 caaattattg actgtaaaag aagtcgttca aattttggtg gacaaagaag tcatttctga
     7081 aaaagtagca gaagttttac ttattccgcg gtactggacg atagaaaccc ctttagtaga
     7141 ggatgcaccg actatgctat ttgaagacct agtgaagatt gcaggatatg aactgcatta
     7201 tcaagaaaac ttggaaactt atgaaaacga atattggtta accaaattag agaactaaaa
     7261 aggagaaaaa aatatggctc aaaattacta tgacgaattt gtcaaattac ctttggataa
     7321 aatggcacaa aaaatggagg atatgacgtt cctatacaac gaaacacgtg ttccaaaaaa
     7381 acattataaa gaaaaattgt cggtagctgt ggaagaaatg attgaataag gtgtcgaaat
     7441 gaatcttatt gcgacgtact atcgaacact ggaagaactg aaaaagcaaa atgcaaaatg
     7501 gtttttccaa gccttactat gcttggaagt aggtgtcaaa ccaagcacta ttaaaccttc
     7561 tgaataccaa gctttagaac taacgtatgc gaaatttatc gaaacgaaaa aagccaagac
     7621 tgtttcaagt gaatggttag attattttga aaatatcaat aagtatggcg catattacac
     7681 catggaaaag gaagacaacg aaaatgaata aggtgattta tcaactaaca acggattcca
     7741 tatcaacgat ttgaaagaaa aaaaggtcaa taatcattta taccgaaaag tgagaatgtg
     7801 tgaaacaagg acaaaaaacg cctagtaact aaggaaatgg ttcttatttc gcaccaatcg
     7861 ctccaaccgc ccccgccgct taggtggtgc gaaataaacg ggcaaagccc gataacttaa
     7921 gagaaagaag gtgatccatt tgaacaagat tattccctta ttgttagtac tgtttgttgg
     7981 cgaaatactt atgcgagata gtgcagctgt acagttaaac ctcgctgtat ttttggtaat
     8041 actggtgttt tgtttgatct tttggcggtt tgtattagtc ctcgtgagtc tactattcaa
     8101 gatgattgca gccatatttt tcatagggtt atttcgatga aaggagtgag taccaatgcc
     8161 aattgcaata ggaaataaac ggttacccgt gacattagat gaaaagagac aaaaagaatt
     8221 gcagcaacta aagcagaagt atggcaaaag tgaatctaag attatgtgta ttgcgttaga
     8281 tctattgatc gcgcaagaaa aagcaggatt tgatgtacca gcgctaaaaa agtgacgtca
     8341 ctttttaatt tgatctatat aagtgatatc acttttacca aggagggaat taaatgttaa
     8401 aaactaaaaa gaattcgtac aaattaaagc ttagtatact atctattatt tgtcttttag
     8461 gcatgataat tagtggtcat ttgtatagag tggcatatac gcagtttatt gagtacaagg
     8521 ttcactcttt aggattagct ggggatcgat acgttgaata tgtccattat tcattaaaat
     8581 gttcttcata tattcttctt tggcttattt tttcattgtt tgaattgcta aatttaatag
     8641 caatatcttc atatcactta gataagctaa ataccaaatc tagccgttat aacgaagtgt
     8701 ttaaggaaaa tgtgaagtta gaaagtgact taaacaagct aaaaaaataa aaagaagaaa
     8761 acataagtta actaaaatta ttttattaag aatgatggaa tataaggata aggttaccaa
     8821 cataacgatc attacttctc tgatatgcta tggacttcta tcaatcagta ttttgtctat
     8881 agattatgga gtaactatac tgcaagcctt agttttggct atgcctatgt atctatcttt
     8941 gtttatcatg catgaaatat ataagctatt tgttttatca aaaaatgtag aaatacgggt
     9001 aaatattttg gtgaatagtt atcaaaaaga actcaaagaa agaagacggt ggcggtattt
     9061 attgaaagga tgaaaaagta ggatttgaca tactggtatt cagaaagtgc cgtcactttt
     9121 aaaacaatgg aaaaggtgga gataagatga agaaaaaaca aactaaacta tcattagcag
     9181 atatttaaca cagttaaccg caactgaaga tggtgttgtt gaatttgaac aatctgaaat
     9241 tgcggtagta gatgatcgtt accttaaaat gccttacttc tttgaccaag caaaggtcat
     9301 ttgcctttgt cactacgatg gggtacgtga ttttaggcgt taaaaagtag tgattatatc
     9361 aactttacga gtaaatataa atttagacat gaaactgaat tatccaatgg aaaatcatgg
     9421 tttataatct agaaaaatta tagtaatgat tggattatat atattctttc gtattttcgt
     9481 acaactgtag cttgtcaaaa ttgagaaatt taaccgatta ggtactttac atcgttattt
     9541 gggtaatttc taaaacatga agcttaccaa cgaataaact gagactatct tattaattga
     9601 tcaatataag aaaatatctt tgaggagatg atgtactatg ttttcgtcta tccacgattg
     9661 gttaggtggt cttttcggcg gtggaggtaa aggttttta
//
LOCUS       DASHRQ010000083         9605 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.087, whole genome shotgun sequence.
ACCESSION   DASHRQ010000083 DASHRQ010000000
VERSION     DASHRQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.087"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            1..268
                     /locus_tag="U2M88_002451"
                     /pseudo
     CDS             1..268
                     /locus_tag="U2M88_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
     gene            807..1007
                     /locus_tag="U2M88_002452"
                     /pseudo
     CDS             807..1007
                     /locus_tag="U2M88_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1494..1673
                     /locus_tag="U2M88_002453"
     CDS             1494..1673
                     /locus_tag="U2M88_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374792.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1719..2324)
                     /locus_tag="U2M88_002454"
     CDS             complement(1719..2324)
                     /locus_tag="U2M88_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM8374793.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2506..2841
                     /locus_tag="U2M88_002455"
                     /pseudo
     CDS             2506..2841
                     /locus_tag="U2M88_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3006..3233
                     /locus_tag="U2M88_002456"
                     /pseudo
     CDS             3006..3233
                     /locus_tag="U2M88_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3359..4330
                     /locus_tag="U2M88_002457"
     CDS             3359..4330
                     /locus_tag="U2M88_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8374794.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICKENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4470..5783
                     /locus_tag="U2M88_002458"
     CDS             4470..5783
                     /locus_tag="U2M88_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEM8374795.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5826..6692
                     /locus_tag="U2M88_002459"
     CDS             5826..6692
                     /locus_tag="U2M88_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM8374796.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLMPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6689..7537
                     /locus_tag="U2M88_002460"
     CDS             6689..7537
                     /locus_tag="U2M88_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM8374797.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7551..9023
                     /locus_tag="U2M88_002461"
     CDS             7551..9023
                     /locus_tag="U2M88_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEM8374798.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            9040..>9605
                     /locus_tag="U2M88_002462"
     CDS             9040..>9605
                     /locus_tag="U2M88_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM8374799.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PYPKDDFAGSCPFHGNCLEGMAAGPAIE"
BASE COUNT         3033 a         1625 c         1985 g         2962 t
ORIGIN      
        1 atctatattt tgatatacaa attacagctt cagatactaa gttgcataaa ccttttccgg
       61 accccataca aaaggcaatt gatgaattgg gcataaaaaa taagaaacga tttatattgg
      121 tgattcatta tatgacatgc aaagtgcgaa agcttgtggg atagcatttg gtttagctaa
      181 atggggagcc aaaaatatag aaaattttca aagtgtagat ataattattg attctcctca
      241 agaaatactt gatttgttag aaaaatagac aaagagcgtg agataaggat agggtctcac
      301 gctctttgtt tttcgccatt taaagattga aatttttgcc atttaaaaag aaaagtgaca
      361 gataggaata aaaacttatt aaatgtagtt atgatctgtt atatttcatt tctaatacta
      421 ggtcttccac aagcagtgtt tcctgaaatg ttttctggag actggggtat tgtcagctat
      481 tctatattaa cattgattgc tatggtttta ggtacttcat tttataatgc ttttgtaagt
      541 aagcatgttg aggaatatga agtattaatg tacttaaaag agaaaaaaac aagtaattaa
      601 tatgagaaca ccacgcatag tttttatttt gagatattct agaatgaaac acattaagaa
      661 cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc taagtttttt catatttgtt
      721 tgttatgcta tacatagatc tgcataatta gacttgcgct gcccgttatt gaagtttcag
      781 gcagcgctct ttttggggga tcgataatgg aagaagaaaa aatatttgag aaacgctggc
      841 agctagcatc aagtgaacaa agagcaagat acaataattt gatcacctcc taccccactg
      901 ttgactagac atataaggaa aagaaatact tactttggct gtgccagtta gatatagata
      961 cctttgaaac atttgaagtg atcctagata aaattaaaca gaattgatca gaaacaagcg
     1021 aagccttctc tcggttcgct tgttttgcta tttattagtt ggctcagggc aactctaagc
     1081 tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta ccactttaga atacatggat
     1141 gtacgtattg ccattgactc ctattagtta ctgaaaaaaa atttaaacaa gttgagtatt
     1201 gcagataaat agctccagtg aggtgaataa ctgctccact caaggcaata gatagctcca
     1261 tccagggcaa caaactgctc cactttcata tttaatagat gaataacctt ctgtataata
     1321 gaaaggcata cctttcggta tgacaactta tacagaatgt tattttattg gttcactatc
     1381 gaaaaatttt ggagctttat ttttccggta tttctcaacg aacgattagt ttaactgtgg
     1441 gaagctccag acatacgatt cgagaagtta cttctcgagc agacaggaag ggggtgactg
     1501 aactaaccgc tgatatgagc gatacttggc tgcaggaata tctttttccg gaaaaacaaa
     1561 caacagcacg aggttacctt cctgtagact gggaatatgt tcataaagaa ctaatgaaaa
     1621 agaatatgac attgaaactc cttcatagag aatatggggt acatggaaca tagaaacgaa
     1681 aatgcacggt agaaaagcga atgaatatag tataaattct acaaatcatt cgctttcttt
     1741 attttatata tggttggtcg agagagacca ttttctttgg caatttctgc aactggaact
     1801 ccttgattta acatgtccac cacagtttta aagactaatc gtttttgcgg atctgttgag
     1861 ttctcagaat attcaattgg tcgcccctta tatcgaccag cttttttggc caataatact
     1921 ccttgccgct gacgttcacg gattttctca cgttcgtttt gtgcaatata acttagcaat
     1981 gataaaaaca tatcgaacat agcagtatct aggagttcat ttccagtatt aaaatctaaa
     2041 aactgtgcat ctagtatttt taatgctact tttttctgtt tcataaaggt aactgtattt
     2101 tttatgtctt cataatctct gcctaggcga tccagtgatg caacaatgac acaatcacct
     2161 tctcggatat aatttagtaa tttttgaaac tcaggtcgct ggatatcttt accactggct
     2221 ttttcttgga ataatttatc aatgttaagg tcttccatca ttgccaattg tcgatctaaa
     2281 ttttgatctt gtgaagacac tctaatatat ccaatttttg ccataaaaac tccttttaaa
     2341 aaatgatata atttacatgt aaaatttact tgtagtttta ctgttatcat agcattttta
     2401 aaaataaatt ttaagtctac ttataattta ctttggaggt tttgaatgaa tgaaattaag
     2461 cgcaataaaa attcttttgg tgtttattta ggagagccta gctcggaaca gttaaataaa
     2521 ggtgaagctc gtcatgctct atgtcgaaat atattttatg gtagaaaagg caaactttat
     2581 caaacttatt acgacggtat ggaggaacag ctcaacgcat taggattagt aactaatgct
     2641 atcatatatt ggaattcact atatttagaa aaagtctttg aacaaattaa aacggaaggg
     2701 ttcgactgtt ccgaagaagt gttacgcaag caatttccat taattagtga tcacattaat
     2761 tttgttggca aatatagttt ccgctatgaa aaagaattat cgaatggaat actacgtccg
     2821 ttaaatatag agaattattg agaatgttcg tcttttggtt cctaccgtgc attttcgcac
     2881 ggatgttcca gatactattt ttgaatgtat aaaattgaaa acacaagaat actaataaat
     2941 caacgattct tgtgttttta atttttcact accgtgcatt ttcgtttcta tgttccatgt
     3001 accccaatct tgaaaaatta attaaagact tagataaagc cattgacgac ttagtcgtgg
     3061 tgctaccgga gtatcaatgt ttaaccagta tcccaggtat tggtcctgtt taggctgctg
     3121 gaatactcgc tgaaattggt ccaattgaac gtttttctga tgaaataaaa ttgacaaagt
     3181 atgccggcct atattggcga cagagtcaat ccggtaaatc tgaatacgaa aattttttta
     3241 cgaaaagctt cacgtctaat atctatttta aaataaaaat attcctgcta atttggaatc
     3301 gtttccttct tttggtataa taatacctga aattcttttg agaggagttg ttcgtgaagt
     3361 gaaaactact atgaaggatg ttgctgcgtt agcaggagtt ggtgtgggta cggtatctag
     3421 agtaataaat ggagtaaagg tgaaagaggt taccaaagaa aaagtaatgg ctgcaattag
     3481 tgagcttaat tatgagcctg atgaatatgc cagaggtatg aaaacgaata gtagcaatac
     3541 aatagctctt attttaccta cgatttggca tcccttcttt tccgagtttg cctactatgt
     3601 agaggaagct ctcagccaaa aaaattataa gctttttttg tgtaatgcga acggcagtgc
     3661 ggaaaaagaa aaagaatata tccaaatggt caagcagagt aaagtagacg gaattatagg
     3721 tattacctat tcagacattg ataaatatgt atcatcaaat ttaccatttg tcagtataga
     3781 ccgccatttt tcggaacagg tttcttatgt aacggcagat aattatttag gtgggcaaat
     3841 tgccgctcaa gaactactta ctcgtaactg taaaaacttg tgctatgttg ggggtgtttc
     3901 accttaccct aacgaaacaa acaatcgtaa agctggtttt tacaatattt gtaaggaaaa
     3961 taaaatagct gtgaaggtat tggatatgcc tgaaccaatt agcaatttag aggaacaatt
     4021 gctcgctttt ttttcggata atcctgaaat tgatagcatt tttaccatca atgatttcat
     4081 ggctttggat gtgataaaag cccaaaaaaa actagggaag gaagcaccta aggattttca
     4141 aattattggt tttgatggag tcaagctatc gttgcaaaaa gactatttac tatcaacgat
     4201 tgtacagccg atagaggaaa tggccaaggt ttcagtagaa acactcatgg cgatgatttt
     4261 aaatgaagaa ccgcctaagc gtgtggtagt accggttttc tttcacgctg gagagacgac
     4321 taaagagtaa aaaaccggtt tataacgatt tttttgagat aggttgacag aaagtgattt
     4381 caatgatatc ttaaaaccaa ttaattggaa acgttaccaa ttaattgaaa aataaaacgg
     4441 aaacgtttca aaaaatagga ggaacatcga tgaaaaagag aatgttagca attgcagcaa
     4501 ttttagcatt aacagctgga ggacttgcag gatgtggcag taaggagaaa agttcaggta
     4561 gcagtgatcc aggagaaatt aagatttggg ttcagttttc tgatgaaacg cctgaaggaa
     4621 aagcttgggc agagattgtg aacaatttta atgatgaata cgagggtgaa tataaagctg
     4681 ttactgagta tattcctcgt agcggaagcg gcggtggata tgaggataaa gtaaatgctg
     4741 caattacaac caataccctt cctgatgtta ttacgttaga tggaccgaat actgcagctt
     4801 atgctcattc aggagtcatt gctccattag atgattattt gaaagatgcg gacatggatg
     4861 atgtattaga cagcattatt cagcaaggaa cctacgatgg caaaatgtat gcgtttggtt
     4921 tctcagagtc cagtgtgggt gtctattaca ataagtcgct gttcaaagat gctggaatta
     4981 cagaagatca attgccgacg ttagaagatc cttggacttg gacggaattt caagcaattt
     5041 gccaaaagct gactgataaa tacaaaacgc ctgcaattga tatgcaaatg gcgtcgggag
     5101 acgaaatgct gacctacgca tatacaccat ttatatggtc gaatggcggc gatgtcgtta
     5161 atgctgatgg aaccaaagcc gaagactatt ttaacggtaa aaaatccgtt gaagcgttgg
     5221 aatttattca agaattggtt gaaaaaggtt acaccacaat cagtcctatt gaaaaaggat
     5281 ttgaaactgg caaatatgcc atgatgctga gcggttcttg gacaatggca gatttagagg
     5341 aaaactatca agagattgat tatggtattt tgccttaccc cgtttcagat gcaactgggg
     5401 agttagtttc accaaccgga agctggcagt tagcaatgac tgagaaaaca gagaaaaagg
     5461 atgcttctgc ggcctttatt aagtttgcaa ccagtacaga atctagtcgt attatgagtt
     5521 tagggaacag tgttctacct atcagaaatt caacagttga tttaattaaa gatgaagtgt
     5581 cggaaggaat gcaggtatta attaagcaaa accaaatttc tggacatgcc cgcccagtgg
     5641 ttgtagctta tccgcaagta tctagagcat tccaacaagc agttcaagat attagctact
     5701 tcgaggaaaa cccagatgta caaaaagtag cagatagtcg cgctgctgaa atgcaacagg
     5761 caattgatac ttcactaaaa tagaagtttc taaagggggc caatcaagtg ttaaaaaaac
     5821 cgacaatgaa agacaatttg ataggttacg gctttctagc gccagctttg ctgctgctga
     5881 cgatttttct gatcataccc gtaatcatgg taatttatta tgcctttact gattattatt
     5941 tgttaatgcc agatgctaga caatttatcg gcttagaaaa ttttaagcga ctgttcgctg
     6001 atccaatttt tgtaaaaagt atttggaaca cggctaaatt tgttgtttgg attattccgg
     6061 tccaaatagg ggcagcttta ggaatggcac ttattatcaa caaaaatcgt aaaggaaata
     6121 tcttttttaa agtagccttt tttgcgccag tcgtgatgtc cttagtcgtt atttcgattt
     6181 tatggttgta cttgttaaat cctaatgaag gtctggttaa tacgctattt gcaaagattg
     6241 gaattgcttc gcagcctttt ttgactagtc ccaaacaagc aatgtatacg cttgtgttcg
     6301 tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt tttaggggga atgcaaaata
     6361 ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt ttctaagttt cagcaatttc
     6421 gctatatcac catgccatta ttaaagccca cggcgatttt tattttactt accacattga
     6481 tttcggcctt taaactaatc attcagccaa tggtaatgac tcaaggtgga ccaatgaatt
     6541 ctactatgac aatggtttat tacatttacc aaactggatt tacagaccgt atggtaggct
     6601 actccagctc cattgcgtta atctttacca ctatgattgg tttaattaca attgctcaaa
     6661 gaaaattggt aaaggaggat gatccagcat gaccaagaaa tttggaccgc taatgatcgt
     6721 agaatattgt ttgctgttcc ttttagcggc attattcatt ttcccgatgc tgtggatggt
     6781 agtttcctct atgaagcctg aagcagatgt ctatcaaaat ttagcttcct tcaaagcttt
     6841 tttacctagt ttgaatccag ctaattggtt taaaacttat caagaggtta tttcccggtt
     6901 cagtgtggga acttatttat taaacagtat tttttatggt ttaacctttg cctgtgggtc
     6961 aatcattgta aactctttgg cgggttttgc ctttgcgaag gttaattttt caggcaagaa
     7021 aattatcttt ggagctttgt tagctctgct gattgtcccg atggagacgg ttttaattcc
     7081 gcaatttacc atcgttaatt cactaggctt agtgaatact cgcttggcag tgattttacc
     7141 agcgatggcc agcgcgttta atatctatct gtttagaaat ttctttatcg cgattccaga
     7201 agaaatcatt gaatcagcga aattagatgg tgcaaatatt gttaaaattt tctttcacat
     7261 catgctgcca atgtctaaac cagccatagc cacagtaggt gtcttatcct ttatctccag
     7321 ctggaatgat tatatttggc cgttaatggt cttaactgac agcagcaaat tttcaatgca
     7381 agtggcaatt actacgatca atactacaca gccagtttat atcaatcaag tgatggctgt
     7441 tttaaccatt gcaacgattc cactgattat tgtttatatc gttgcacaaa aatatattct
     7501 gcagggactt ggcggttctg gaacgggtat aaaataggag tgaatagtga atgttgcaaa
     7561 aacttttaaa ggccaatgag tttattgaga aaaatgctca aacagtcaat caagaatacc
     7621 ggaataaatt tcatttgatg gctccgattg gctggatgaa tgatccaaat ggttttgttt
     7681 attatcaaga ggagtatcac ttgttttatc aatattatcc ttacgacagc atctggggac
     7741 caatgcattg gggccatgct aaaagcaagg atctaattca ctgggagcat ttgccagtag
     7801 cactggcacc aggtgaaagc tatgacaagg aaggctgttt ttccggcagt gccatagaaa
     7861 aggatggcaa gctgtatttg atttatacag gacacaatgt tgtggaaggg caagttcgac
     7921 aagttcagtg tttggcagtt tcagaagatg gcattcactt tgaaaaatat gccggaaatc
     7981 caatcatcgc agaagatcac ttagcttctg ttgcaacgac cgaagatttc cgcgatccca
     8041 aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc tactaaaact gcagatgctc
     8101 gtggacaaat tgtgatgttc cattccaaag atttactaac ttgggagttt tattccgtat
     8161 tgctggaggg aactccagaa caaggcattg tatgggaatg cccagacttg tttgaactag
     8221 atggaaaaga agtcttgatt ctttctccta ttcaaatgac tccacaaggc aatgcttacc
     8281 aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga ttggcaaaca ggcaaatttc
     8341 tagtagagaa ttttcatgaa atcgatggcg gtttagattt ttatgcaccc cagacctgtg
     8401 aaaacgaagc aggccaaaga ataatgattg cttggatgca aatgtggcag cgtaaaatac
     8461 ctacgaatga tctgggccat ggttggtcag gttccatgac cttgcctaga gagctgaggg
     8521 tggctgataa tcaattgttg caacggccag tttcatcaat ttatcaagta attgatccgc
     8581 aggaaaggat tgaatgggcg aagactgaag agaatcgctt cttactgaag gacttagtat
     8641 atgaccagca ctatttacgc ttggaaatca atttaaaaca ggcaacgaaa ctagaacttc
     8701 actatgcgaa gggacccgac catacattac tgcttaacta tgatgttgaa cgggagtgct
     8761 ttactgcttc cagggaacac atgccgtatg atttatcagg agctgaaaag gaagtgttga
     8821 aaaagcgcag tgtgacgatt cctttggaaa ataattgtct aaccatcgaa attttccgtg
     8881 atacttcagc tattgagctt tttttaaatg atcgtctgac tatgagtaca actttttatg
     8941 agacagaaaa aggacaggat tttcttttgc tggcagacag tgaagtaacc ttttcattgg
     9001 aaagcggctt aattaatcta tagaagggag agctcagtga tgatttatgg ttcaatcgaa
     9061 gcaggcggca caaaatttgt gtgtgccatt ggcgatgaag aaatgacgat caaggaaaga
     9121 gtgagctttc cgacaacgac acctgaggaa acaatacctt tagtcattga cttcttcaaa
     9181 caataccaag cagatttagc agggattggg attggctcat ttgggcctat cgatatccat
     9241 cgcgattccg ccacttatgg gtacattacc tcgaccccga aattggcatg gcaaaatttt
     9301 gatttcatcg gtaccatgaa aaaagaattt ccgatcccga tttcttggac aacagatgtc
     9361 aatgcggctg cctatggtga gtatgtcttc ggcagtggca aaggattgag cagtgtcgtt
     9421 tactatacga tcggtacggg tgttggcggc ggtgccttac aagaagggcg ctttatcgaa
     9481 gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc cttatccaaa agatgacttt
     9541 gctggcagct gtccatttca tggcaactgt ttagaaggga tggccgctgg acccgccatc
     9601 gaaaa
//
LOCUS       DASHRQ010000084         9090 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.088, whole genome shotgun sequence.
ACCESSION   DASHRQ010000084 DASHRQ010000000
VERSION     DASHRQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.088"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            244..687
                     /gene="rplM"
                     /locus_tag="U2M88_002463"
     CDS             244..687
                     /gene="rplM"
                     /locus_tag="U2M88_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEM8374800.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            703..1095
                     /gene="rpsI"
                     /locus_tag="U2M88_002464"
     CDS             703..1095
                     /gene="rpsI"
                     /locus_tag="U2M88_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEM8374801.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1184..2410)
                     /locus_tag="U2M88_002465"
     CDS             complement(1184..2410)
                     /locus_tag="U2M88_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM8374802.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2482..2640)
                     /locus_tag="U2M88_002466"
     CDS             complement(2482..2640)
                     /locus_tag="U2M88_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8374803.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            3328..4068
                     /locus_tag="U2M88_002467"
     CDS             3328..4068
                     /locus_tag="U2M88_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEM8374804.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGETNYIESSKN"
     gene            complement(4160..5347)
                     /locus_tag="U2M88_002468"
     CDS             complement(4160..5347)
                     /locus_tag="U2M88_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEM8374805.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            5669..6511
                     /locus_tag="U2M88_002469"
     CDS             5669..6511
                     /locus_tag="U2M88_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEM8374806.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            6511..7458
                     /locus_tag="U2M88_002470"
     CDS             6511..7458
                     /locus_tag="U2M88_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEM8374807.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            7473..7706
                     /locus_tag="U2M88_002471"
     CDS             7473..7706
                     /locus_tag="U2M88_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEM8374808.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            8037..8621
                     /locus_tag="U2M88_002472"
     CDS             8037..8621
                     /locus_tag="U2M88_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM8374809.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            8752..9090
                     /locus_tag="U2M88_002473"
                     /pseudo
     CDS             8752..9090
                     /locus_tag="U2M88_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
BASE COUNT         3032 a         1398 c         1626 g         3034 t
ORIGIN      
        1 aggggttgtg acaagacttt tgtcacagcc tctttttgtt tttcattttg acaggaaacg
       61 acccttttat ataaaagaaa gaaaagaagt aaagtttcat ataaattttt caatcaagca
      121 gaaaaacgtt gacattgact agattagcgg gtaatatgtt aaatggtatt gtttgcccca
      181 caaagagccc cggaaactcg aagtgtatgt caaacatcag aacttaaatt ggaggaaaat
      241 aacgtgcgta caacatatat ggccaaacca ggcgaagtag aacgtaaatg gtatgtagta
      301 gacgcaactg atgttccatt gggacgtctt tcaacagttg ttgcatctgt actacgtggt
      361 aaaaataaac caactttcac acctcatttg gatactggtg atttcgtcat cgtcatcaat
      421 gcagacaaag tgaaattaac aggtaaaaaa gcgactgaca aaatttatta ccgtcacagc
      481 aactacccag gaggattgaa atcaatcact gctggcgaat tgcgtgctaa aaattctcgt
      541 cgtttgatcg aaacttctgt aaaaggtatg cttcctaaaa acactttagg ccgcaaacaa
      601 tttacaaaat tgaacgtata cggtggagca gaacatccac atgcagcaca acaaccagaa
      661 gttttagaca tcacaaactt aatctaatag gagggaattt cattggcaca agttcaatat
      721 atcggcacag gccgtcgtaa aaacgcagtt gctcgcgtac gtctagtacc aggaactggc
      781 aaaattactg taaataaaaa agatgttgaa gaatacattc cacatgctga cttgcgtgaa
      841 gtaatcaatc aacctttcgc tgttactgaa acaaaaggcg catatgacgt atttgttaac
      901 gtaaacggcg gaggatacgc tggacaatca ggagctatcc gtcatggtat cgctcgtgca
      961 ttgctagaag ttgatcctga cttccgttta gctcttaaac gtgctggctt acttactcgt
     1021 gacgcacgta tggtcgaacg taaaaaacca ggtctcaaaa aggctcgtaa agcatctcaa
     1081 ttctctaaac gttaatatta tttatatatc aatgtttcaa ggcacttttc aattgtttga
     1141 aaagtgtctt tttcttgttt aagaacacat ttaagaacac atactaatca catttattca
     1201 atcctctctg aaaggcttca aaaacagttt tatctactac atctggtgta tttacgtata
     1261 ttctcgttgt agaaatatct gagtgtgtta aagcactaga tatttgattc atatttgctc
     1321 cgccttgtct agctatagtt gcataggtat gacgaagctt atgtggagat aaatgagcca
     1381 attcaggatg tcgtctttta attgaattga ttcgatagtt taggtaatca gcgtgaacag
     1441 ggcaatttac ttctccggat ggtttagtat aggtaaagag atactgatct ggtgtttgat
     1501 taatttttaa tctcaacaat tgctcagctt gcactgattt ccatttgctt agtaatgcca
     1561 ttacaatttc gggtacttga attacggtat cctttcgacc ttttgtaaat ttcttttttt
     1621 gatatttatc caaagcatgt ttaaggcgaa ccgtttgttt ctcaaagtcg atatatttcc
     1681 attgcaaagc atacgtctca ctttttctat caccaagata tagggtaagc atgaaaagag
     1741 tatagtcatg aagagtaagc aagtgattat tcaagtcagt gtttactgct tctatccaag
     1801 caataagctc ttctgacgat aatgcttgat taccttcccg tatccttctt tcttctaaag
     1861 caattttttt aggtgcagtg atacattgaa taattttggt agttcggtta aattcaatat
     1921 aatttagtat ttctgcaata tcaaacatag aacggacata tcctttgata atttttatat
     1981 ttgcgtattc tttcgatttt tttgttaatt catctgatat tatctcagta tttgaattta
     2041 gatagttcat tgcatactca ccaaacattg gaaggatgtg caatcggaat atatctttag
     2101 tattactaat tgttacgtaa cttggagctc tttttgatct agtagtctga cctagttcat
     2161 atctaggaag ccattttgat tcatagaatt ccttaaattt tatttttcct tttaattcta
     2221 gtgaatttgc attctcctct cttaaaactg tttcaatttt ccttttcatt gcattttcgg
     2281 ctttaatagc caattgccta gttggaaata ctttttgata cttttctgat gtaattccag
     2341 tgatttctct gaattttttt ggatacctca gattagtttt atatttccca tttgaaagtt
     2401 ttgtaggcaa aattcctcac tcccttaaaa agtaaggcag tatatttcgc tattcttagt
     2461 atatgcggcg agaagaaatt ttcaaattgt tttaaatgta ttcataaaat ctttgatatc
     2521 ctctatgtca aagcgaacta taccgtcaat tctgattact ggaaaattat atatacgaat
     2581 ccacttatct aaagtattat tatcaattcc tagaaactca caggtttgtt ttttattcaa
     2641 gtagactctc aattttataa aatcatctcc taaaaaagat tagaattttt gaattgcttt
     2701 gggtaagcac ataggaattt cacctctccc cagtttacgg cgagccttac ggaagtatca
     2761 ttatttgctg ctattgtcat ggcattttgt tttcccagaa caaaaagaaa tctctaagta
     2821 tttgtcgcta atcaggtcat ggcgtacttg agtttctagc tcgaatagca gggatcgtta
     2881 ttcaaaaatt gtatttaatt gtcaatgatc aaaaataggt tttttcctat tcactattaa
     2941 taacgataaa catgtataaa aagacacact acgaaaaaag ttaaatggag tttactgatt
     3001 ttcatatttg tatatggaac agacatagtc ttgaataatt tttataaatg ttttgatgct
     3061 tatcaactta ttcaacccgc tatccacttt aagtataatt tttccatacg ccaataaagt
     3121 agttttagga gcatgttctc atttgagaac gctccttttt tatatatttt gaaagctact
     3181 tactatacgc gtagtttcaa atagttttgt cattctaatc ttcagcaaca gaaaaaaatc
     3241 taaattataa aatttagggt tgactaaaat ataatttttg tataccatat gatcgtgata
     3301 atcattatca tttagaagga gaaagttatg ttagaagtga aaaagcttaa tgtttgttat
     3361 aacgattttt tagcattgaa cgagatttct ttaaaaatac aagaaggtgc tttaacaggt
     3421 attattggtc caaatggtgc tggaaaatct acattgctta aagcaatgtt aaatattatc
     3481 cctcatcagg gagaggtctc cattaataat gaagatatca ataaaaaact ggtgaagatt
     3541 gcatatgtag agcaaaaagc tgatattgat tttacttttc caataaaagt aaaagaatgt
     3601 gtatctatgg gaacatatgc cggaatgaaa gtttttcaaa gaattaaaaa tgcagaatgg
     3661 caaagagttt caaaggcttt ggaaaaggtc gatatgggaa aatattctaa tcatcaaata
     3721 ggagaattat ctggaggaca atttcaacgt gtacttttag caagatgtct tgctcaaaat
     3781 gcggatttca tatttttaga tgaaccattt gtagggatag acttagttag tgaacgaata
     3841 attatggata cgttaaaaga attgaaacat cagggaaaaa cagtgctaat tgtgcatcat
     3901 gatttaagca aggtaaagaa gtattttgat aatattattc ttttgaatcg aaatctaatt
     3961 gctcacggaa gtgtagaatc tgtatttaat gaagagaatt taaaaaaagc ctatggagat
     4021 actatattta ttggagagac taactatata gagtcaagta aaaattagcc aaatcaaaaa
     4081 atattttata acagataatt ccgaaaaaag ggtacaacaa ataagcttct taccattagt
     4141 ttttttgaat gttcaatagc taatcagaaa caattatata aggctcgttt ttagtcaaaa
     4201 tagcatgaat cacgtaacaa agcttacgag aaaccgcacc taaggcagtt agatgatgtt
     4261 ttccttcgga aacttttttc tggtaaaacg cactaaaaac aggatcacat tgagaagcta
     4321 ctaaagccgc cgaaaacaag gcttttcgca aatatggaga ccctcgttta ctcataacat
     4381 tatgcgttgc ctcatattgc cctgattgtg tcacggaagc gtctatccca gcgtaagcaa
     4441 cgagtttttt cgggttagaa aatttttgaa tatctccgat ctcaccaaga atagtcgcac
     4501 cgtttatagg accaattcca ggaattgtga gaataacaga atttaaagtg tccattatgt
     4561 gcttgatttc tctttccgtt tcctttattt ggctttcaat gaattttaat tgttcaatca
     4621 ttgaacgtag ttggaaggtg aacgcttctt gagcaaattt tactccaaag gagttagaag
     4681 ctgcttgctt caattggtcc gctttttctg ccttgattcg gttcctgctt aactcagcta
     4741 agtattgggc caaagtttct gaggaaatgt tttctaagtc gatcggggca ctaaacgcca
     4801 gcaataactc tttagaagct tttccgaaaa cgccctgctt ggtaaaaatc gtcgcgtatt
     4861 ctggaaagac ttgatccaat acggctataa tttttctttt gaagtcgctc gctgtaccta
     4921 ctaaataagt tcggtaacga gaaagttgtt tcaaagaaaa aacattttca tcggataaga
     4981 ccgtttcgac aaatgagcca tagcgcatta agtcagcaat caaaacagaa tcaataatat
     5041 cgttttttcg tttccgtatt tctgtacctt tacgccatcc gtctgtttga aggggattca
     5101 caacgtgaat caaataatct gattcgtgaa ggtatgaaaa gatggacagc caatagtgcc
     5161 ccgtggcttc catccctata acaaagtcac tgggtgctcc ggaatagtcg accattcgct
     5221 caattaatga ctctgctcct tcagtggtgt taggaaatga aaaacctttt agtaatacct
     5281 ttccttcctc gttcatgata gaagcaacgt gagtgcgttt accgatatca atgcctaaaa
     5341 agtacatgta tatctgacct ttcaaaaaaa gaattttgga ttgaaatcca ctcaactgaa
     5401 aagcgtcact accttgtacg agatacggag taaagtaagg actttcaaca tctaactcat
     5461 acgcaatcca cttatcagag agaggagcca gtctctcgag tgcgggttca atggcccaag
     5521 gggggaacgg cttagcctct caatccaaaa aatatattat ctcaaaagaa atgagttaat
     5581 accaaattaa aataacgatg gttcataggg ttatccaatc aaaaccggaa ggttaaaacc
     5641 ctaactctaa tatacaaggg ggaataatat gcttcaaaat tttattaatg gtctgtatga
     5701 ctttcacttt ttacagaatg ctctaattac atctgtagtt atagggattg tttctggaac
     5761 agtaggttgt tttattattt taagaggaat gtctcttatg ggtgatgcta tttctcatgc
     5821 tgttttgcca ggagtagcaa tttcttatat tttaggtatt aattttttta ttggtgcgat
     5881 tgtatttggt ctattagctt ctatcattat tacttttata aaaagcaata gtgtaataaa
     5941 aggagataca gcaattggta ttactttcag ttctttttta gcattaggag ttatccttat
     6001 aggagttgca aatagctcaa cggatttatt tcatatctta tttggaaata ttttagctgt
     6061 acaagatatt gataagtgga ttacaattgg cgtttctgtt ttagtgttgg ttacagttgt
     6121 tctatttttt aaagaattat taatcacttc ttttgatcca ttaatggcaa aagcaatggg
     6181 gatgaatgtc agtttttatc attatctatt aatgattcta ttaactttag tagcagtaac
     6241 agctatgcaa agtgtgggaa ctatattaat tgttgcaatg ttaattacac cagctgctac
     6301 agcatattta tatgcaaaca gcttaaaaac tatgatattt atttcggcga cagtcggagc
     6361 gtgctcatca ttgctaggtt tgttcattgg ttatagtttt aacatagcag caggatcaag
     6421 tattgtttta acagcggcat tcttatttgt aattagtttt ctaatctcac caaaacaaaa
     6481 atttttaaga aagaaggaga gaaagttatg atgagaaaat ggaaagcagt actgggaagt
     6541 ctggggattt tgattgctct ttttatattc ggggcatgtt caacaaatag taaagacaaa
     6601 gatacagtgg cttcgaacga aaaattaaag gtagtagtta ctaattcgat tttagcagat
     6661 attactgaaa atatagcaaa agataaaatt gatttacaca gtatcgtacc tattgggaaa
     6721 gatccccacg aatatgaacc tttgcctgaa gatgttcaaa aaacttcaaa agcagatttg
     6781 attttttata acggtgttaa cttggaaact ggaggaaatg cttggtttac aaaattagta
     6841 aaaaatgcga acaaagagga aaacaaagac tattttgcag caagtgatgg catagatgtt
     6901 atttacttag agggtcagag tgagaaaggg aaggaagatc cccatgcttg gttaaattta
     6961 gaaaacggta ttatttacgc taaaaatatt gaaaaacagt tagcggaaaa agatcctgat
     7021 aataaaaaat tctataaaga aaatctagat aagtatattg aaaaattgga ttctctagac
     7081 aaagaagcta aatctaaatt tgcttcaatt ccgaatgata aaaaaatgat tgttacaagt
     7141 gaaggatgct ttaaatattt ctcgaaagcg tataatgtgc cttctgctta catttgggaa
     7201 atcaacactg aagaagaagg aacaccagat caaataaaac acttagttga aaaattacgc
     7261 acaacaaaag ttccctcctt attcgtagaa agcagtgtgg acgatagacc aatgaaaaca
     7321 gtatcaaaag ataccaatat tcctatctat tcaacgattt ttactgattc aattgcagaa
     7381 aaaggacaag atggtgatag ttactatgcg atgatgaaat ggaacctgga taaaattgct
     7441 gaaggccttt cgaaataatt tggagggtag gtatggtaca aattaaaacg actactatac
     7501 taatttattt actagcaatt caggaattat caaaaagaaa aaagaaatta aaaaataatg
     7561 atttagcaaa agttctaagt gttagtccag catctgtaag tgagatgctg gctaaactta
     7621 gatatgagga ctatttagat ataggatttc aattaactga taaagggaaa aaatttattg
     7681 acgattacaa aaaaggtttt aagtaaaaga ctttattttt agggaggata agaaaatggc
     7741 tattggagtg attatgatag gacttaccct aatagctgga tataattttt ttaaatcttt
     7801 ctcggattat tttagaaaag gtgaatgatt tagatgagtc tattaattct tgtaggattg
     7861 ctttttgtat cagttatttt gctctttctc attagtttag ctctaaggaa tgagttcagt
     7921 aatagacgtt aacgattgaa acttcaagtc ctgtgtaaga taatatagtt aattcatctt
     7981 attaggagga agtacccata gaaaccagag attatattac aactcaccaa aaattaatag
     8041 aaagtggact aagccatttt gaaaaagaag gatttcagca aactaagata aggcaactgt
     8101 gcttgaccgc aggcgtaact acgggtgctt tttataaaca ttttacttct aaggaagcgt
     8161 tatttgaaga aattattcag ccgcatatta atgcgcttat taatatgtat aatgaagagt
     8221 ttaccaaata cctcttaaac ttagaaaata tagatataga gaaaagttgg agagagaact
     8281 tcaatgaatt agatccattt attaattata tttttgagaa taaacgtata ttcgatttgg
     8341 ttttattcca atcagctgga accaaatatc aagatattgt tgaaaaaatt acatcatttg
     8401 tgacaaaaaa gacagtctgt tgtctagata agttaaagac tatcggaatg atcaatcaaa
     8461 atttagtctt taatgaagaa gaaatccact tctacattta ctcattctac gctacattct
     8521 atgatatatt gaagcatagc tattcaaaag aaaagactat gctattaacc agaagattgt
     8581 atcaattttc tgttcctggg tggattgaat tactgagtta attaaaaaaa agcttgcatt
     8641 gagaattgtt ctcagttatg ctttttgata agatcgcact gttatctgaa taacggtgca
     8701 tttttgtcag cttaagataa cagtgcgatc ttattttttg gaggtgaaag agtgtcaaaa
     8761 atcaatccgt taaccttgat tactttaaat atttttttcc ggtcattatt tttttaggaa
     8821 agggaaatat atttcaagtg gggtgtttca ttattgctac agttttagta ttgatgtttc
     8881 gtaaaataaa gacatgtatt tatttcatct tgatttattc agttctgttt ttagtaatga
     8941 aaattattga tgctatcaag atagagtttt ttactatatt ttttgggaca tccttatata
     9001 ttgtatttcg aatgttgccg gtcgtaatga tctcatggtt gttggttagt gtctatacta
     9061 gcaatgaatt attatcctct ttagaaaaaa
//
LOCUS       DASHRQ010000085         9086 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.089, whole genome shotgun sequence.
ACCESSION   DASHRQ010000085 DASHRQ010000000
VERSION     DASHRQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.089"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            62..595
                     /locus_tag="U2M88_002474"
     CDS             62..595
                     /locus_tag="U2M88_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374810.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(721..1398)
                     /gene="nth"
                     /locus_tag="U2M88_002475"
     CDS             complement(721..1398)
                     /gene="nth"
                     /locus_tag="U2M88_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEM8374811.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1405..2103)
                     /locus_tag="U2M88_002476"
     CDS             complement(1405..2103)
                     /locus_tag="U2M88_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEM8374812.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2371..3555
                     /locus_tag="U2M88_002477"
     CDS             2371..3555
                     /locus_tag="U2M88_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEM8374813.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3561..4004)
                     /locus_tag="U2M88_002478"
     CDS             complement(3561..4004)
                     /locus_tag="U2M88_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM8374814.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4230..4517)
                     /locus_tag="U2M88_002479"
     CDS             complement(4230..4517)
                     /locus_tag="U2M88_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374815.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4688..6040)
                     /locus_tag="U2M88_002480"
     CDS             complement(4688..6040)
                     /locus_tag="U2M88_002480"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEM8374816.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6313..6759)
                     /locus_tag="U2M88_002481"
     CDS             complement(6313..6759)
                     /locus_tag="U2M88_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEM8374817.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6995..7921)
                     /gene="cysK"
                     /locus_tag="U2M88_002482"
     CDS             complement(6995..7921)
                     /gene="cysK"
                     /locus_tag="U2M88_002482"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEM8374818.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(8061..8948)
                     /locus_tag="U2M88_002483"
     CDS             complement(8061..8948)
                     /locus_tag="U2M88_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEM8374819.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTITSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2498 a         1836 c         1481 g         3271 t
ORIGIN      
        1 cttttattta gtgccacttc ttaataatcc cattgttatt atcaaataaa aaatacaata
       61 tatggttttg aataattttt tgaaagaagg cttcattttg agttatctga tagtatgctt
      121 aattatcata atttgtctgc accatctttt tttatcgaaa acaatacctc gttattccaa
      181 gaataaaaaa gctgaaaaat tttgtttatt attaaataaa tttactttaa ttgctcctat
      241 ccttgcattt attatttttt ctgtactttt aagcactact cttaaaggaa aatttatgga
      301 aagaagctct catgcaatga ttctcacttt tttatggttg ttgttcacca gaatctatat
      361 ttttctgatg agcctgaaac cgcccaaatc cattagttta tgtttagtta taaatggtat
      421 ttttttatta tcacttatca tttttattac acctttagat agatacgtca cttatttata
      481 taatcctttg gaatactgga cttattttat aggtatattg gaaggcatta ttttctacat
      541 cgggtatttt cccaataaaa ataacaactt ttatttttat cgcaataaat tgtaataagt
      601 taccatccta gctactgagt atgtacattt actttttcat gcaaaaaaga taaacgaaac
      661 agcttttctc cataataaca agataattca tacatttatt tgcaattaaa gaaaccacgt
      721 ctacaggcgt ggtttctttt ttagcgcctt ttcttttaga cgcatccgat ttttcccatc
      781 ttgacatatt gatagcaaag gacatacctc gcacttggga tttcttgccg tgcaatgata
      841 tctaccaaaa aaaatcagtg tatgatgtgt ctttacccat aattcttgtg gtactttccg
      901 catcagtgtt tcttctactt ccatgaccgt agcatctagt ttgcagatcc tcaggcgttt
      961 tgagacacgt tccacgtggg tatctactgc gattgctggt atcccgaatg catcccccaa
     1021 tacgacattt gctgtctttc tgcctacacc aggaagagac atcaattctt ctcttgacgt
     1081 tggcacttgc ccatcgaaac gttcaatcag ctgctgtgca cacgctttga tgttttttgc
     1141 tttatttcga tacaaaccaa tcgtttttat tttaagaatg atttcatcga ttgaagcttc
     1201 tgctaacgca tcgggtgtcg gaaaagaggc aaacaaatct ggcgttgctt tattcactga
     1261 tacatctgtc gcctgggcac ttaagatcac tgcaatcaat aactcaaatg gattattgtg
     1321 cttgagttct ccatgtgctt cgggaaacat tccatacatc gtttctagag cttccattgt
     1381 tttttgtttg cttaacatat tccgctactc ctccaaccaa tttttcagtg agactttcgg
     1441 caattcttct tgttgagctg actgttctac ttctctttga agaagctgct gttttcttcg
     1501 tttttggtct tcttccactt gttgttttgt acgtagattt tttctttccc acgatagcaa
     1561 aatgcggtca acgtaattaa aactataagc ctgatttaat acggcttccc gcagtgctag
     1621 cttcaaaatt tccggctgat aatggtcttc ttctagccat tgtccgatcc gctggaactc
     1681 gatcgaagaa agcgggcgtc caaattcttg ttcaaataat tgatacactg actggatttc
     1741 tttttcttgt gttttttctt cgtatttttt ttcttgtgtt tttaaatact cacctaatag
     1801 atcatagatg gggtaaagat tatatcgatc tgctttttta ccgttgacaa tcgtcgtttc
     1861 tatcttgata aatccatttg ttacgagacg atccagtatt tgaaaaatct gatcttgttt
     1921 gattcccata tccatagcaa tcatctgcaa atcagggaaa gtatctcctt caagctgtgc
     1981 catatgcaac tgtagaacaa ataaaaattc ttcagattgt agaccgatcc tatgataatt
     2041 tttaagtaaa agattagata tgactgtttg tccagcgctt aagtaatttt ctaaattcaa
     2101 catgatttct cctccgctca atcacagtat acgtaaaaaa aacatgggaa gcaagaacag
     2161 gtttcttttc tatttacttc actgatcggt cagatttaca atgtcgtcta cttgattttt
     2221 gtttgttcat gtttttgatg cgtatcttca cttttcggaa taaataaatt aagtagattt
     2281 cgctgttcct tagaatgttg acaaggaaac aaaattacta tacactattt ttgtttccaa
     2341 gttaacatta ttgcataaag gagtttttac atgtacagtt tcacaaataa acattctaaa
     2401 gtttttggat acgtctctgt cttattcact ggattaggat ttactatcgt cagtcctgta
     2461 cttccgtttt taacgctgcc ttattctcac tccatacatc agcaagcttt ctatatcact
     2521 ttgctcatgt ctgtttatgc tctagctgcc ttcttatctg cccccattct cggtagctta
     2581 agtgatcatt ttgggcgtca tccgatcctt attatcagtt tattaggatc aagtatcgga
     2641 tatttgcttt ttggtttagg aaattcgata tggatgttgt tccttggtcg tatcattgaa
     2701 ggattgacgg caggagaaat ctccacctta tatgcttact ttgccgacat cacggaacct
     2761 aatgaaagaa ccaaagtttt tggatggatg ggtgctttag tcggtatagg aacaacgctg
     2821 ggtcctatta tagggggtct attagctgag ttggggaatt ctgttcctat atttattgga
     2881 gcactcttta ctttcttaaa tgccgtttat ggctacacat ttatgctaga aagccttcca
     2941 ataaaaaaac gttcagtaga tttaagtttc tcccatgtaa gaccttttca ccaattacaa
     3001 caattattca agatttcatc cgttattcct ttattgacag caggttttgc tgtttggttg
     3061 gcagctggct ctcttcaatc tatcttttct caattttcga ttgacgcctt tcagtggaag
     3121 gctgggttgg ttggtttaag cttctctttg attggaatct tggatactgt ctcgcaattg
     3181 ttcattatgc ctcgattgct caaaaaattt tcagaacagc agatcacaag aatagggatg
     3241 ttcagtgaaa tcctcgccta tctattcatc acattatctg gaattcttct actgccgatt
     3301 ttgtttttat ttgggattat ctgttatggt ttcggtgatt ctatttttac tcctgtattc
     3361 aatggccaac tatccaattc tgtctcagat aaccagcaag gtcttgtaat gggcggtacc
     3421 caaagtatac aatctctatc gcgcgtcatc ggcccgctaa tcgcaggaca gctttatgct
     3481 gcagcaccat ttcttccgac ggtattcgga tttttgctta tgatttttgc ctattatcac
     3541 tacaataaaa aatagtagct tcatttgcta cttcgtataa attctctgta tcgaccgata
     3601 aaacgaaata ctgtgtcaaa ttcttctttt gacatatttt caaatacagt agcatctcgc
     3661 tgtacccatt tttgatggag ttgctcgtgt ctattcctta ttttttgtcc gattggcgtc
     3721 aattgaaaat agatttcttt tttattctct tcgctttgat aagtgtttat gatccctcta
     3781 gctatcagtt tttttgttag cttactgatg gctcctctag tcatgtaact agcattcgct
     3841 aattttgtca cgttaggatc agggattttc ccgatatgtt caatcacttc tagttcattt
     3901 agagtaaatc ctgcaaattc tttttccatt tctggtttat tacgatgaat aatctcatta
     3961 gctaaatcca gatattcttc tattagcagt tgtcttttat ccatttctac accttctttt
     4021 tctttcatta tattgttttt ttgtttccta ttcaacaaag ataaaaatat tttgtaggaa
     4081 aacccaatct gaaaccaaga aaaaaagggg ttgacaaagc ttgtcataac ccctttttct
     4141 gattaaaaga tagtaaagag acagtcatcg gtaattgtga tgattttttg tcatcactta
     4201 aggatgagcg gccttttcaa aaactgtctt taaagatcat catcagtttc tagcacatat
     4261 ggctgacctt catatgtgta ctctgtattc aatgtgccgt cattttcatc tgagtagatg
     4321 cgtctactga aaggaaaacg atgagaaaca ttatctttgg cgtcatttgc tggcatcgtg
     4381 taatattcct cttgcgtttc attcaactga gagagtactt gatctgccaa ctcatcaata
     4441 ttgatcgtta atttcttcga agcactttta taatcttcta aaaatgcatc ttgttggaca
     4501 aaatattctt tattcatctt ctttctcacc tttcttttca aaatcaaata aagctgcttg
     4561 ttcttttatg taacagcttt atctttgctg cttattgtga caaaaaattc gaagaaagac
     4621 aactaattgt acttttcaca aaaaaagaca gagggtcgtg acaaaattgt gtcacaaccc
     4681 tttttcttta ttgtgcagtt tcgatttcgc ctagcgctgc ttgtgcaagt aggttcaagt
     4741 aattccatgg gcggtcaaaa tgtggttgga agaagaaatc tgaaagtgcg agatcttcta
     4801 cagtcatttt attttgaaca gctaaagaca tggtatttgc tgattgtgta atgtcgtatt
     4861 tggacatgaa ctgagcacct acgatacgat tcgttccttt ttcatagact agctccatca
     4921 ttactttttc agtagttggc atgaactctg gacgataatt atcttcaaat acagtcgctt
     4981 ggacttctag atcgttcaaa gttgcgcttt ctttggtcac acctgtcgaa ccgatcgtcc
     5041 agccaaatag gtaaagtcct gaagttcctt gagtaccacg atacgccatt ttatgatctg
     5101 tcaagttatt tcccacaagc aaaccttgac gaacggcatt tgttgccaaa ggaatatagt
     5161 tttgcgtgcc acttggattg tagttgacta ctgcagagtc tccagctgca aaaatgtctg
     5221 gattacttgt ctgcatgtat tcattcactt tgattgcccc gtttggcagt gtatctactt
     5281 ttccttcgat cagttttgta tttggacgga agcccacaca taaaatgacc atatctgtat
     5341 caaatgtatc tgttggtgta gctacttgtt tcacttttcc ttcttcatct gtgataaatt
     5401 cagtaacatt ttcgcctagc gcaagagtaa caccgcggtc tgtcaattct ttttctaaga
     5461 tatctgtgaa tggtttatct agatatttat tcaagatgcg atctaatcca tcaatcaatg
     5521 tcacttgttt tccagattcg acaaaagctt ctactaattc aataccgata tatccaccac
     5581 caacaattac tacacgtttt gcttctttcg cttgtgcaat aatttcattt gcttgattgt
     5641 agtttttgca taggagtaca tttttcgcat cgattccact aatcggtggg ataataggcc
     5701 atgaaccagt tgtcatcact aatttatcat aagaaaccgt ttctgtttct cctgttttca
     5761 gatcttttgc cgttactgtt ttattttctg tatcgacgtc tgttacatcg tgttccattt
     5821 taactgttgc tcctaatgaa gataattctt caggatttga gtagaacaag cctgctgggt
     5881 cctttactac tccgccgaca taaagtgcga taccgcaaga taagaaagaa atattgtcat
     5941 ttctttcata tactgtcact tctgcttctg gatgattttt taaaatactt tttacagctg
     6001 cagtacctgc atgagtacag ccaattacta ctactttcat ttatggtccc tccgattaac
     6061 attttgtcct tcgacaaaac tactataaca gaaataaaac ggatacaaca gtttaatgct
     6121 tgtgaattca caaaggtttg tttgtagttt cattcctttt caagcataag aacccaatat
     6181 ctacaagatt cttattttaa taatattcac ttttatatct aagattgtga ataaaagaca
     6241 aaaaaataaa aaagctagcg ttttttttac acagctagct ttttttaggt ataacacttg
     6301 tagaaactgt ttttacattt gcgccttttt tgctgactct ttacaatccg gacagagtcc
     6361 atacaattcc aaccgatgtt cattaatctc aaaatcagtc agtctgcttg cagccatttc
     6421 tatatcttca agccctgggt aatgaaaatc aacaatctta ccgcagtttt cacaaattac
     6481 atgataatga cgctttgaac tgaaatcaaa tcggctagaa gcatcgccgt acttcatttc
     6541 ttgaacaaat ccaatcgaag taaataagcg aaggttattg taaacagtcg caacactcat
     6601 atttggaaac cgatgttcaa gtgctttata aatctcatca gccgttggat gggaatgatg
     6661 ttcaatcaaa tattctaaaa ccgcgtaccg ttgaggtgtg attcgaatgt tctctttttt
     6721 taacttttcg ataacctctt cgactaactt attttccatg taaacacccc acgttgttat
     6781 tctcgtctta tcatactatt tttacatttt aaaatccact taacatgaaa agaaaattca
     6841 attttattgt attttaatga aaaaagacgt aaaacagtcg cgttcactat tctacgtctc
     6901 tattcaaata atttttctca tcataaattt ttgtgtgttc ttttcatctt taaaagtttt
     6961 ttacataaac ctatctggag aaaaaccaag tgccttatat ctcttggtaa agggctgtag
     7021 ataaataacg ttcaccattg tcgggaacca cagctaatac cttcttgcct tttcctaatt
     7081 cttttgcgac ttttagcgcg gcagcaattg cagcgccaga tgagatgcct actaaaatcc
     7141 cttcttttcg cccaacttct ctagctgttt ccatcgcttc gtctccacta atagaaagga
     7201 ctttatcata cacagaagta tccagtgttt taggaacaaa acctgtaccg attccttgga
     7261 ttttgtgagg tcccgcttct ttcccttcta aaatagcaga ttctgctggt tctaccccga
     7321 ttaattcgat tttcggatac acacgtttta attctcggcc agcgccagtg atcgttcctc
     7381 ctgtaccgat acctgcaaca aacgcgtcca agccatttac tccaaatgct tgatgtatct
     7441 caggaccagt cgtcttctca tgcacaagag gatttgcttc attttcaaat tgtaatggta
     7501 aaaagtagcc gttttctttt gccaaacgtt ctgcttctct gattgatcca gaaataccat
     7561 ccgcaccagg agtcaaaatc aactctgcgc cgtacccttt catcaataaa cgtctttcga
     7621 tactcatcgt ctcaggcatc acgatgatta ctttatatcc ttttgcaact cctaccatcg
     7681 acaatccgat tcctgtattt ccagaagtag gttcaatgat agtatcacct ggtttcagta
     7741 gaccatcgtg ttctgctttt tctatcatac ttaaagcaat acgatctttt acacttcctc
     7801 ctggattaaa aaactcaagc tttacaaaaa catctgctga atcttctggt acgattttat
     7861 ttaatttgac gattggcgtt ttgccaatca attcggtgac tgagttatag atagctgtca
     7921 tcttcaattc ctccttgtct ttgctcttct tacttatcat aatacaatct gttcatttat
     7981 ggcgagtgat tcgtctttga ctagcaaaaa aaagacaaag atataccata tatggtaatt
     8041 tctttgcctt tttatctaaa tcaaacgttt agttcttgac caactaataa agaataactg
     8101 gacatagaca tgccattttt cgccattaga tcatcaacag acatgccaaa tttttgtgca
     8161 atatcccata gcgtatcacc tgacacgact gtatatgtct gaccattatt tgtatttaca
     8221 tctgttgttg aagatgatga cgttgaagtc gtcgtagtag tcgttgttgt tgtacttgtg
     8281 attgttgtag ccgaacctgt tgatggcgta tcaaaacggg ttaaattata agtattaatc
     8341 aatgagttca atttggcccc ataatttgga tcggtagcat aacgacctgt caagtacgct
     8401 gttgcatcat aaaaacttgt tgtattgctt ttccacacgc cagcataatg ataatcgccc
     8461 gtggaaaaac tggttgaacg aagaacattc gcatggtctt ggaaggattc ccaataagaa
     8521 gaatattttc ggaatggctc attcatcgtt acccattgtc cgtttacata ttctttggtt
     8581 gccatataaa cgacctgtcc gttatagctt cctttcacac cgaatagatt ataatttggc
     8641 gcactagcta atccggaagt gccatatgca ctttccaaca acgcttgagc gatcatgact
     8701 gaagcataca agtcattact tgcagcaact tctgacgcag accagccgat ttgatcgata
     8761 aacgcttgag gatcagcgac tgtttgagat ccttgttcat ctgcatgaac atgttgcggt
     8821 accgctgtaa ccatcatcgg cccaactaaa agactcgttc caattaaagg agcaatcgct
     8881 tttttattga tcaccatttt tcttttaggt ctatggtgcc gggcactgcg ggtcttcagc
     8941 tcttgcattc aaattcctcc tcaaaataaa aaaacttaat aaaaagttct cttcttaaaa
     9001 caaacttaat taattaaatt atacttttaa ttccgccgtt tgtataatta agcgagacaa
     9061 ataaaaaagc catttatgtt acactc
//
LOCUS       DASHRQ010000086         8965 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.090, whole genome shotgun sequence.
ACCESSION   DASHRQ010000086 DASHRQ010000000
VERSION     DASHRQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.090"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(56..349)
                     /locus_tag="U2M88_002484"
     CDS             complement(56..349)
                     /locus_tag="U2M88_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEM8374820.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(377..1042)
                     /locus_tag="U2M88_002485"
     CDS             complement(377..1042)
                     /locus_tag="U2M88_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEM8374821.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1182..1814)
                     /locus_tag="U2M88_002486"
     CDS             complement(1182..1814)
                     /locus_tag="U2M88_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8374822.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1821..2888)
                     /locus_tag="U2M88_002487"
     CDS             complement(1821..2888)
                     /locus_tag="U2M88_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEM8374823.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2885..3616)
                     /locus_tag="U2M88_002488"
     CDS             complement(2885..3616)
                     /locus_tag="U2M88_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEM8374824.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3785..4264)
                     /gene="greA"
                     /locus_tag="U2M88_002489"
     CDS             complement(3785..4264)
                     /gene="greA"
                     /locus_tag="U2M88_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEM8374825.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4400..5575)
                     /gene="mltG"
                     /locus_tag="U2M88_002490"
     CDS             complement(4400..5575)
                     /gene="mltG"
                     /locus_tag="U2M88_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEM8374826.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            5779..6948
                     /locus_tag="U2M88_002491"
     CDS             5779..6948
                     /locus_tag="U2M88_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEM8374827.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(7215..8849)
                     /locus_tag="U2M88_002492"
     CDS             complement(7215..8849)
                     /locus_tag="U2M88_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374828.1"
                     /translation="MRWYMNLKKFFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTLKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTI
                     RYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         2856 a         1701 c         1333 g         3075 t
ORIGIN      
        1 atattttcaa aagaaaaaag agaaggtgac aaaaattgtg tcaccctctc tttttttatt
       61 gttttttaaa gtgataggtt ggctcctcca ggttgggatc ataatcaaca agtaaatcat
      121 atcctttaaa gaaccattca tctgcttcct cgataaaaaa aagtgtccca tcaatttcca
      181 ctttctttac aggatgttct ggctgttcaa ctgacattcc aatcgaaaaa ccatcatgaa
      241 cctctgtttt gccgtagact tttccaaaaa aacgtattcc gtaagtttct ggcagatcaa
      301 tttctgagtc aaaccacttt tgtgcttttg gcgttaccgt taatttcatc ccaacaacct
      361 actttctctt ttctttctat tgattcatct tatcgtctag aatatccaca tcgcctgtct
      421 ctccaataac cagtagatta tcattttcac gaacatattc atctgctgca ggagaagcga
      481 taaccgtttg atctactctt cgtatggcga ctactgttaa gtcaaaacgt tgcctgaaat
      541 ttaattcagc taatgttttg ttgaaaaatt taggattgga gactcggatt tctgctaaag
      601 aatatttatc tgacaactct aggtaatcta aaatattctt tgacactaaa ttatgcgcaa
      661 tccttattcc catgtctcgc tcgggatgga cgacgcgatc agctccgatc ttttcaagta
      721 ctctggcatg atactcattt tgggcttttg ctaaaatatt tggtacgccc atttccttta
      781 ccatcagagt aactaagata ctggcttgaa tatcttctcc aatagcgaca attacatgat
      841 cgaaatttct tatgcctaat gatcgaagtg tcatttcatc ctgtgcattt gccacaacag
      901 catgagtggc tatattcata tattcattga cccgatcttc attactatca atagccagta
      961 cttcttgacc agcttcgatc agtgttcggc agatacttcc gccaaatcgt ccaagaccta
     1021 caattgcaaa attttgtttc accgttttcc tcctttagta tcttcttttc tcagtataac
     1081 aaagccttta caaaaacaaa agcaagcatc attattgtaa aaaagccaag aaaaataata
     1141 gttattttct cagcttttga ttatcctctt tatcatcgtt tttatttcac caaaccatgt
     1201 ttgaatgcat agatagcggc ttgtgtacgg tcttccactt ctagcttagc caatatattg
     1261 gaaacatgtg tttttactgt tttcagtgta ataaacaatt cgtccgctat ttcctgatta
     1321 ctttttcctt cagaaatcag cattaaaatt tcattttccc gattcgttag ttcttcatgt
     1381 aatacaggct catttcgccg actcattttt tccatcattt ttgttgtcac ttctggttct
     1441 aatactcgct cgccacgttg cgttgccctt atagcatcag caatttcatg tgcggtagag
     1501 gtcttcaaca aataccctgc tgcacctgct tcaatggcag gataaacttt ttcgtcatca
     1561 ataaaactgg ttacgataat gattttagct tctggccaat cttttaagat tgcttttgtt
     1621 gattcgatac catccatttc ttccatgact aagtccatta aaatgacgtc cggtcgaagt
     1681 gtcatcgcct tctcatagcc ttgtcgtcca ttttcagctt ctccaattac ttcaatgtct
     1741 tcttgaatag ataaataaga agagacgcct aagcgcacca tttcatggtc atctactaat
     1801 aaaactttta tcatccttat ctatgcctcc ttcatcaaag ggaccttgat ttctacactt
     1861 gtaccttgac ctttaaaact aatgattttt actgttccac caataccagc tactcgttct
     1921 ttgatattgt tcaatccata acttcccgtt ttcgtttcat tcatattaaa ccctgttccg
     1981 tcgtcaataa tacgtaacaa aagattattg tctattttgt gcaaatatac ctctaattca
     2041 ttggctttag catgtcttaa tgtatttgat aacaattctt gaacgattcg gaacaagtga
     2101 tcctcaatgg aactagatag ttttacatct tctacatccc atttcagcga aatctggatt
     2161 ttattttgca attccttcaa taactgctcg ataccttgct ttaaactttt ctcttctaaa
     2221 ttgactggac gtagatgaag aagcagcgca cgcatctcag actgggaagc gttgatgatc
     2281 tctgctacca ttttcaactg cttgcgaaac atttctggtg tctcgctttt ttctgcttgc
     2341 tccgtcaatg ccgacatcat catcattgct gcaaacaatt gttgcgaaac tgaatcatgc
     2401 agctcacggg ctaatcgatg tctttctaat tctaaaattt cttctttggt ttgtccatct
     2461 acatattggg gacggctcgt tacaatttgt aattcactag atacttcaat cattttttct
     2521 tttatcttag tgatttcctt atcaatatct ttgatgtata aatcgtccga tgcacttggg
     2581 atcggaagaa gcaacaattt ggattcataa ttgccagaag aaagtgcacg cagcttttct
     2641 tcgattttcc cgtattttgt cttttgaata agtgataata atagaaagac aattaatcct
     2701 acacctaagg aaatggctaa caaatggaag ataagcggaa catataataa acgtgcacgc
     2761 agtaattctc cccaccaatg actttgattg ctggcataaa aataagtgaa aagtgaaaat
     2821 agaataataa ggaaagccgc aatccccgaa taaacagcta gcatcgctct ggatattttt
     2881 cccatcatat tcgtatcacc tcgacatctc caatcaaggt attagtaata atcttcaatc
     2941 tacgagggtt ttcatcataa tcactgctgt agattttgat ttgttcattt ttcaaagaaa
     3001 attgctcttc ttcaaacaag acatttccta ctaatgtcgc atgctctaat cggatggcta
     3061 cgcccaaagg tactaagatc cgtgttctgc ctatcccttt tcttacaatc acaatattat
     3121 catcttttgg caaaagcgta ttgcccaaat ctatgatagt atcaccagaa atgacggcga
     3181 tgttgatatc gtcccattca tagacgtcgt ttccaatacg ttggttaccg aataattgct
     3241 gtcttttccg ctcattatta tgtgttttta attgctctgt ttggatcatc atgatctgtt
     3301 tctttcgcca aaaagcattt ttggttaaat ctatccctga aatctctaca cctttcagac
     3361 caataaataa aacggcaaat acagccatca tccataaagc aggactgttg actaagctaa
     3421 aaaaaatgac caaacttcct aatatcaatt gaaaattttg aaatttagta cgaggatgtt
     3481 tgcgcatagc tagatatata ttgaaaatac cgaatatcac taaaagcaaa agtccagtat
     3541 tgtttacgat ttgccaaaca gcaaataaca gcagtagtgc ttcaactacc acaaaaaaac
     3601 gccaagaact attcatcgct ctttctccct cctgacacat tctcatttca tcatacctat
     3661 ttttttaact gttggctata ggactttaga tgtaagatcg tttgttgaaa tctaaaatac
     3721 acaaagagaa aaagatctag agtgtatcat cagtaagatg aactctctag atctttcagt
     3781 cagactacgc ttgttctact ttgacgattt taactaacat atcgccacct ggagtagcaa
     3841 tagttacttc atcattttta cgtttgccaa tcaatgcttg cgcaatagga gaatcattgg
     3901 aaattttgcc agaaaacgga tctgcttcag cgcttccgac aatcgtgtac tcttcttctt
     3961 ctccatctgg tagttcgatg aatgtaactg ttttaccaat tgatacctca tctgcatcta
     4021 caccgtcgtt atcgatgatt tgagcaaaac gaatcatatt ttcaattgtt gtgattcttc
     4081 cttcaacaaa tgcttgttca tctttcgctg attcatattc agagttttcg gaaagatctc
     4141 cgaaaccgcg ggcaattttg attcgttcaa tgatttctcc gcgtttgact gtttttagtt
     4201 cttctaattc tttttctaat ttttctttac cttctagagt cattggataa actttctcta
     4261 ccatgtgtaa cgtctccttt tttcgataaa ataccgcaaa aggtaatctt ttgaggaata
     4321 tagtccaaaa aagattacca cgcttttctt caaatgtaaa tgtttttcta aaaatgatca
     4381 atgtctagag cctgtttttc taagaattat ttacatattt ttccactaat tccatatgtt
     4441 cttcataagt tttagcgaaa taaacatttc cagtgctcgt atctgctacg aaataataat
     4501 agtcattttc tgctggttct aatactgcct tgattgcctc ttcgcttgga ttgtcaaaag
     4561 gtccaggtcc atagccggta ttggtataca aattatatgg tgaatctaca gctgtatctt
     4621 catacgtcac taattctttg tgttcaccta atgcgtataa aacagagata tctgactgaa
     4681 gtggcatatt tgctttgatt cggttaaaga agacttgtgc aatgttttta cgatcatttt
     4741 cttttacgcc ttctttttca actagagaag ctagagttaa aacttcttgt acagtcaaat
     4801 ttttctgctt gatagctgaa taatagtttt gcatgaccgt attcgtcttg ttcaccattt
     4861 caatcactag atccttaagc gaagtactcg tgtaataatc atatgtggct gggaataagt
     4921 aaccttccaa acggtatttt gtgttctcag ctttagatgc actttccaac agttctggga
     4981 atttttggtg caactcgtta aagaaagtct catcattcac taaatctaaa aattctttct
     5041 tgtctttacc agttaccttc gctacccgtt cagcaatctg atcgatatcg tacccttctg
     5101 gaatggcaat tttcccatct gcaacttttg ttggctcaga agtaccgcct tcttgcaact
     5161 gtttgccaat ctcgtcaaga gtcatattag gcgctaactg ataatagcca gcttgaaagc
     5221 cagttaaatt tttaaatttt gtataataat tgaaaacaat cccgctttta ataatattgt
     5281 ccttctccaa aatctcacca atctgcttgt tagaagagcc actcgggatc tcgacttgga
     5341 ctaattggtc atctgattta tctaaaggct tcaacccgct atctacataa cgatacacgg
     5401 taaatccaag aaatccgcca ataatcaata atgtaagagc aatcactaaa atgatcttcc
     5461 tcacaatctt atcttctttt ttacgcagat ttctccgctt gtcgattata tctgctgtag
     5521 ctgccttact ggcttctttt ttatctttac ttgggtgttg tttgtttcct gacaaaacaa
     5581 ctcctcctta ctatcaagtt cgtaacttta ttcattatac atgatatttg aattggcgtg
     5641 aatgatttta tttaaatatt ctatcaaaaa tgtaagtagt aaatccatta ctataagatt
     5701 aaaataaatg ttatcaagat acaagctatt ttagctttat tctttctgta aaaaagaaag
     5761 gagactaaat aaactattat gaaaaaagga aaacatgcta aaaaaagaaa atattcattt
     5821 ttaatgatcg ttgtcgtgtt gtttagcctt agtgcgttct ctacaggaac tattcttgga
     5881 tggcaatttt acaataatga acactctcag atagttgccc atgctcaatc agctcaaaaa
     5941 aagttgatac tgactaccaa tgaacaacag ctcgttgaaa aaatcgatca actattaaga
     6001 aaaaatgatt atattggctc aatttacgta aagaaaaata atcgcatcat catggaaaaa
     6061 ggctatggct atgcgaataa actcacgcaa caaccaaatg atccttctct ttattatcag
     6121 attggttcgg ttcaaaaagc attgactact cttttgattt taaaacaagt cgagcgtggg
     6181 cacctttctt tagatactaa gctggctcaa ttctatccac aaattgctgg cagccagcaa
     6241 attactatcc gtgacttgct gtatatgcgc tcaggcttgc accgaactgc ttctccaacc
     6301 ataccgatga ctgatgaaga agtcgttcaa tttgcgattc agcacttaaa atttacagat
     6361 tatcagacct ctcattatga ttcattgaac tttaccatat tgactggaat tttgatcaaa
     6421 ttaacaaatc aaccgtatga aaaattattg accgatgata tcatcgaacc tttaggatta
     6481 cagcatacaa aattttatga ccaagtcaaa aattcatcca atcatgcact atcctatcaa
     6541 atgtctaaag aggatgatta ctttaaacca ctgacagaat ctgaaacaga gatccataac
     6601 gaattaggga ctggtaatat cagtatgtcc gtctatgatt taaatacgct gtttacacaa
     6661 gttttatctg gaaaactagt ttccaaagac ttactcttct cattatggcg tgaaaatgcg
     6721 aatggacatc catatagtgg tggggtatat agcggagata attatatcct gtcgcaagga
     6781 aatatcgact cttttgatac atctgctata tttagcaaag attcacagga tgctatcatc
     6841 atggaaagca atataaaagc tgacaaaact atcaaactgc ctactgctga tctaagaaac
     6901 caaatctatg acctgatgga aggaactact aagttggcaa atagctgata tgatgaaaag
     6961 atagcaaata ctacttcttt gaatagcatt tgcttaaaaa aagtactcga ctaaacgtag
     7021 gaaagagtgt gggacaaatc taaaaatcaa tttttgctac agactttaat tccaaataaa
     7081 cagaacagaa agaggtgttc ttctttctgt tctgtccaaa ttattctaag atttctcttt
     7141 accaacatta tttgacaaag ggcctattaa atctaaaata gtatctttag attgctaaaa
     7201 tatgtgctag caaatcattc atggcttgtt cttattttta attttatgca ataaaaaaga
     7261 agaacaatta gcgtgattat agaaataaat aatgagattt tagaaaaaaa tgtgtatgaa
     7321 tacctgattg ttactttatt tattccactt tcacttttta ctgtaacaaa aggacctcct
     7381 tgttttagta ctgtagcatt ttttgaatta acagtaaccg ttaatccttt ataccggaat
     7441 acaggtaaaa ctattgtttt gccttttgaa gatttaagcg taaatgtaat ttcatcttta
     7501 tttggagaaa agctactata aataacttga ccatcttctg taacttgatg gtttttcaaa
     7561 agcaattgat cttttttata gcctataggt acataatcaa aattctcaaa ttcttcaagt
     7621 ttattttcca tttgttcatt agaataagtt ccttgaaaat tcatttcttt caccatgtta
     7681 actactgatg aataatttaa aattgaaata gctaatatta ataatagtat aaaatttctt
     7741 ctccttgtat aagaaaattt ttccaactgt ggagaaataa atagagatac tgaaaaagat
     7801 atgaacaatg ttgaaaatat cataaaacgc caaggagctt gaataatatt taaaggagta
     7861 ttttgtaagg caaaccatgg aaatattttt gtacaaagta gaatagatat tatacccatt
     7921 atataagtga ttttagataa gattcctaat tttttaaagt taaatgttag tataattaaa
     7981 caaataagca aagttaagcc aataccaaat gatagtaatt ctgaatctat acttccaatc
     8041 aaaaactctt tacccagata aattgctctt ttttgtaata tatccacata aggtctattc
     8101 agatcttgat acaatgtctg ctcaatcata ggacctataa acgctagtga aagaagtaaa
     8161 gtaactatag cagcttttat taatttaata attaagaaga tttctatttt ctttttccga
     8221 taaaattgaa taattaaagc aattataatc attaccatag tcatagctac agaaagaaca
     8281 tgagtgtaaa ttaataaagt catgctaatc gccaacataa cccaagattc tttttttgag
     8341 tcgtaaagct ttataaaccc gagtaaaata agaggaagta ttgacattgc gagtaattcc
     8401 cccattgccc ctcgaagaaa tatttctaca ctcctaaaag aagatgttgt atatataata
     8461 gcgaaaaagg agctagtaac atgttttttg gtaatttcat acattacata atgacatata
     8521 aatagagtaa tgagcgtaac aatataaaga aaaaggttat agcctattgc taaattagta
     8581 aacttgatca ataaaaataa aggatataga gttaaccatg ggtaaaaatt atttacttgc
     8641 atgccatatg atctaaacgt ttcaaaatta ataggggagc taaaaataga ttctaaactt
     8701 aacgcacgat taatgtgaaa tgccgtatca taaaagccaa atatttccat gtttttaaat
     8761 gctggaaata aatataataa actaacaaat gcataaacta taaatatttt tacagaaaaa
     8821 aattttttca aattcatata ccacctcatg tttttttata ttacatatag tatcatttat
     8881 ttttttaaaa cactagatta gtcactatat aaaatgtgat taattttatt attaataaaa
     8941 tattttttat attacaaaag tagtg
//
LOCUS       DASHRQ010000087         8731 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.091, whole genome shotgun sequence.
ACCESSION   DASHRQ010000087 DASHRQ010000000
VERSION     DASHRQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.091"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            2..74
                     /locus_tag="U2M88_002493"
     tRNA            2..74
                     /locus_tag="U2M88_002493"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="U2M88_002494"
     tRNA            78..150
                     /locus_tag="U2M88_002494"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="U2M88_002495"
     tRNA            155..225
                     /locus_tag="U2M88_002495"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="U2M88_002496"
     tRNA            238..311
                     /locus_tag="U2M88_002496"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="U2M88_002497"
     tRNA            323..394
                     /locus_tag="U2M88_002497"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /locus_tag="U2M88_002498"
     CDS             660..1853
                     /locus_tag="U2M88_002498"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEM8374829.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="U2M88_002499"
     CDS             1983..3455
                     /locus_tag="U2M88_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM8374830.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="U2M88_002500"
     CDS             3538..4326
                     /locus_tag="U2M88_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEM8374831.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="U2M88_002501"
     CDS             complement(4393..5061)
                     /locus_tag="U2M88_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEM8374832.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="U2M88_002502"
     CDS             5176..6888
                     /locus_tag="U2M88_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8374833.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="U2M88_002503"
     CDS             6891..8663
                     /locus_tag="U2M88_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8374834.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2622 a         1487 c         1944 g         2678 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc a
//
LOCUS       DASHRQ010000088         8662 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.092, whole genome shotgun sequence.
ACCESSION   DASHRQ010000088 DASHRQ010000000
VERSION     DASHRQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.092"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(180..737)
                     /gene="frr"
                     /locus_tag="U2M88_002504"
     CDS             complement(180..737)
                     /gene="frr"
                     /locus_tag="U2M88_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEM8374835.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(740..1462)
                     /locus_tag="U2M88_002505"
     CDS             complement(740..1462)
                     /locus_tag="U2M88_002505"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEM8374836.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1598..2479)
                     /locus_tag="U2M88_002506"
     CDS             complement(1598..2479)
                     /locus_tag="U2M88_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEM8374837.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="U2M88_002507"
     CDS             complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="U2M88_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEM8374838.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3719..4198
                     /locus_tag="U2M88_002508"
     CDS             3719..4198
                     /locus_tag="U2M88_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEM8374839.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4415..5506
                     /gene="phnW"
                     /locus_tag="U2M88_002509"
     CDS             4415..5506
                     /gene="phnW"
                     /locus_tag="U2M88_002509"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEM8374840.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5499..5627
                     /locus_tag="U2M88_002510"
     CDS             5499..5627
                     /locus_tag="U2M88_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEM8374841.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5631..6176
                     /locus_tag="U2M88_002511"
     CDS             5631..6176
                     /locus_tag="U2M88_002511"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEM8374842.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6630..8321)
                     /locus_tag="U2M88_002512"
     CDS             complement(6630..8321)
                     /locus_tag="U2M88_002512"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEM8374843.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
BASE COUNT         2410 a         1772 c         1568 g         2912 t
ORIGIN      
        1 acttaaaaga ttcccactat cttaagtatt atagcaatat ctaactgttt ttcctatgaa
       61 atcattgttt ttttatttaa aaactgaata attttttctt tttttaattt ttaaataaaa
      121 gaaatataaa taaaaacaga ggaagcggaa tgatccgccc ctctgttttt atgattttat
      181 taaacttcca ataattcttt ttctttatca gcagcgatct tatctacttc tttgatgctg
      241 ttatctgtca atgtttgtac gtctttttct aatccgcgca aatcatcttc ggtaatatca
      301 ccattttttt gttgtttttt atattcatcg atcgcatcac gacggatgtt acgaatcgcg
      361 atttttgcat tttctgcttc ttttttcaca tctttagcta attcttttct gcgttcttct
      421 gtcagttgcg ggataactaa gcgaatgaca tttccatcat tggttggact gattccgata
      481 tcacttgcca taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc
      541 attaaaacac gtgcttctgg aatttggatt gatgccattt ggttcaatgg tgttggcgca
      601 ccgtagtagt tcacagtaat acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt
      661 tgcccaagtt cacgttgaag attttgtgct gcttttttca ttttttcttt tgcttctgtc
      721 ataattgcat ctgccatttt tatttccccc ttacagttgt tccgatattt tcgcctaaga
      781 ttgcacggcg aatatttccg tgttcattta aattgaagac aagcagtgga atatcattat
      841 ccatgcttag tgaacttgca gttgaatcca tgacttgtaa tcctttggca ataacttcca
      901 gatgagtcag ttcttcaaat tttactgcat ttgcatctac tttcggatca gcagaataaa
      961 caccgtctac attgttttta gccatcagga taacatctgc gccgatttct gccgcgcgta
     1021 atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt accgcctgca aagataacga
     1081 tccgtccttt ttctaaatga cgttctgctt tacggcgaat atatggctct gcgatttgac
     1141 gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac gttttccaac gtatcctgta
     1201 aagctaaggc attcataact gtagctaaca tacccatgta atcagcttga gcacgttcca
     1261 tgcccatttg tgctccgatt tgtccgcgcc agatatttcc gccgcccaca acgattgcca
     1321 tttcaacgcc cagctcgtga acttctttga tttcttggat gatttctttg attactggtg
     1381 gtttgattcc aaatcctgcg tccccagcta aagcttctcc actcaatttt aagactacac
     1441 gctgatattt cggtgtgacc atgttcattc ctccaatatt ttctattgcc attttaccat
     1501 acttagatga tttcgacact ctaagactgg tttttcttgt tttgcttaaa aaaagggagc
     1561 gcacaaaccg tgcgttcccg tctgttattc aacagaatta ttttttcact tggctcataa
     1621 cttcttctac aaagttatct tcgcgttttt cgataccttc gccaacttcg aaacgtacga
     1681 atgttttaac agttgcgcct tttgatgcaa cgtatttttc aacagtcata tcaggatctt
     1741 taacgaatgg ttggtccacc aaagcgattt ctgctttgaa tttcttcaag cgtccttcta
     1801 ccattttttc aacgatattc gctggtttgc cttcgttcaa tgcttgttct gtaagaacag
     1861 ttttttcatg ttctaattca gcttctggaa tttgtgattc gtttacataa cgaggattga
     1921 tagctgctac gtgcatagct acgtctcttg caactgtttc gtcagttgtt ccatctagaa
     1981 cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc accaaatgca gcattgtcgt
     2041 ctttttctac aacttcaaag cgacggaagc tgattttttc tccgataact tgagttgctt
     2101 cgatcaagtc agattcaatt gtacctttat ctgttttgat tttcattgca gcttccatgt
     2161 ctgctggttt gttttcagca acaagttctg caatttcttt tactaaatct tggaacattt
     2221 cgtttttaga aacaaagtct gtttctgagt ttacttcaac gattgctgca gtgttacctt
     2281 tcacagcaac tgaagccaat ccttcagctg caatgcggtc atttttcttc gcagcctttg
     2341 ccattccttt ttcacgtaaa agatcaactg ctttttccat gtcgccttct acttcaacta
     2401 atgctttttt cgcatccatc attcctacgc cagtcatttc gcgtagttct tttaccattt
     2461 tagctgtaac gtctgccatg tttaactcct cctgtgtgtt tttcttttta aaaaaagctg
     2521 cttcaaagga gagtgaggct tgtcgcccag cctttgaaac agctccatca ttcaaaatta
     2581 ttctgctgaa gaattgtcgc cttcaacaac atctacgatt tcttcgattg atgttgctgt
     2641 gttttcttct gtgaaatctt cttcaactac ttgatcttca ccttggtttc cttcgatgaa
     2701 agcatcagcc atttttgaag taatcaattt aacggcacga attgcatcgt cgtttgaagg
     2761 gattactacg tcgatttcat ctggatcgca gtttgtatca accatagcta cgattgggat
     2821 atttaatttg tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa cgatgtacat
     2881 tacatctggg attcttggca tatcagcgat accgcctaag aatttttcaa gacgttcacg
     2941 ttctttgttc aaaccagcaa cttctttttt aggtagaact tcaaaagttc catcttcttc
     3001 cattgcattg atttgtttca aacggctgat acgtttttgg atagtatccc agtttgtcaa
     3061 tgttccgcct aaccaacgat ggttaacgaa gtattgtcct gcacgagttg cttcttcttt
     3121 gattgcttct tgtgcttgtt tttttgtacc tacgaataat gctacgccgc cttcttcagc
     3181 aacgctcttc atgtaatcat aagctgcatc tactaattta actgtttttt gcaagtcaat
     3241 gatgtagatt ccgtttcttt ctgtgaagat atatttcttc atttttggat tccagcgacg
     3301 agtttggtga ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa ttactgccat
     3361 gtttgtttcc tccgatttgg ttttattttt cctcttctta tcttctacct tgccggcaaa
     3421 ctttttcaag cacctaccga caatccaata agaatgtgga tttgctgatg cataaagcac
     3481 cctcagctat tatacagaca aaagatccgt atcgcaagag caaatatact tttctgctca
     3541 acattcaata gtttacctga aagataaaaa gaattcaaga aaaacagtag atttctcttt
     3601 gtttctttat tatccttttt ccataaaata attttaggta taaaagacga atattattgg
     3661 gcaatccttc ttttttttgt tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat
     3721 gcgcaaagct caaagacatt tgctgattaa acaagtcatt gaagaattcc cgatccgtac
     3781 gcaagaagaa ctattaaaca aacttgggca ttatggcgtt accgccacgc aggcaacgat
     3841 ttctcgagat atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg
     3901 cttcgtattg tttcgcgaaa aaggaactcc tgagactagg actgaggaag aggatcggct
     3961 ggttcaaatg attgaagaca ttgtattgaa agtcgaacga gtccactttt tgacgatcgt
     4021 caataccttg ccagacaatg ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc
     4081 ctctattgtc agtacaattg ctggattcga tacgatcatg gtcatctcgg aaaatgagga
     4141 ggacgcaaaa cgagtagaag aatttcttca ccatccaact gaagtatcac ttttttaatc
     4201 agagcacgta ttcctaatat ggaatgcgtg tttttttatt ttcaatattt tccttattga
     4261 ataaaccttt acactcttta caaaaataca tcatttttta ttcaaatttt tcaacacctt
     4321 gattgaatca caataatcat catttatatt ggatgttaac aagagcagct tcttgctctt
     4381 tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa cttctggacc
     4441 attgacgact tctgaaacag tgaaaaatgc tatgctgatc gatcattgta cttgggatca
     4501 agaatacaaa gaaattacac aatggataag aaaaaaatta ttggatcttg ctcaagtatc
     4561 ggaaggaata tacacaacgg tcttaatgca aggtagcagc tccttcgggg tagaatcggt
     4621 attaagttct gtgatcaggc cagaagatac tgtactgatt tgttcaaacg gcgcatatag
     4681 acaacgaatg atcaacatgt gcgaatgtct gaaactgaac tataccgttt atgaggtatc
     4741 ggaacatcaa atcctccaag ctcatgtgat tacacagctg attgaagaga atccgtctat
     4801 ctccgcacta gcaatggtcc atagcgagac tacttccggt attttgaata atttggaaga
     4861 gattgctgct gtcacacaag aaagagatct tttgtttata gtggacgcga tgtcaagctt
     4921 tggtgggata ccgatttgtg tagccgaact agggattgat tttttgattt ccagctcaaa
     4981 caaatgtatt caaggtgttc caggcttttc ttttgtactt gctaataaag cggaactgga
     5041 aaaacgaaaa aatgttagcc gaagtttgtc acttgatcta tatgaccagt accgcgtgat
     5101 ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc catacggttt tggcttttca
     5161 acaagcgctg aaggaactag aggcagaagg aggaatcgaa actcgatatt cacgttatct
     5221 cgccaataat catgtgctac gacaaaaaat gacagagatg gggttccagc ctttcattca
     5281 agaatatcag gggccattca tcacctcttt tctttaccct aaggactggc gatttgattt
     5341 taatcatttt tatcagtatt taaagcaaaa tggatttgtg atctatcccg ggaaaatcag
     5401 cgatcatgaa tgttttcgta tcggaaatat tggcgagatc tatccggaag atattttgga
     5461 actgacagca attatcaaag actataagga gaacgttaat gattaaaact gtaatttttg
     5521 attgggcagg aacaactgtg gactttggct gtatggctcc tgtccacgct tttagaaatg
     5581 cttttctaga aaaaggaacc caactgactg acaaagaaat ccgctaaccg atgggaaaat
     5641 taaaatggga tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa caatggatag
     5701 ctatttatgg acaactacct caggaaaaag aggtggagga attgtatcaa caatttgaac
     5761 attatctgtt taaagatttg gtcaagcatt ctaccttgaa acccgacaca ttggaaacga
     5821 tcgaacggtt gagaaacgct gggatcaata ttggttccac gaatggattt aatgctccta
     5881 tgatgacaat cgtagcagaa accgcaaaaa aagaaaggta ttcaccagac ttcatggcaa
     5941 cttttgaaga cgtcgaaggg tatggtcgac cgtttcctta tatgatccat aaaaacatgc
     6001 agtacttcaa aaacaagtcg gttgatgaag taatcaaagt cggagatacg gtctcagata
     6061 tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt agttcgctaa
     6121 tggggatttc ttatgatgag taccaaaaat tagagatggg gaaaaagatc gtttaaaaca
     6181 gtctgttgct cttagattcc aacaagccgg cgctgatatt tgtgtaaaca atttaaaaga
     6241 gttaacagaa tacattttgt acgctgacaa gaatccagaa tgacttttat acaaaataag
     6301 aggttgtgaa aaagctgtcc tgtatcaagt aataaaaaca gccaagcaaa aatagctcct
     6361 catatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt
     6421 cgaaaaaaaa aagggagctg tgacacgact tttgtcacag tccccttatt tgcaaacaaa
     6481 cagtgtttaa gagacaacct ctccactaaa cggtgttcat tcagctgact ctcggtattt
     6541 gctcaaaaaa cgataaaatt tgaaaagcta ttttctcttt tgtttctcta atactcggat
     6601 cagaacgctg atttcacatt ctctttttct tacatcttat ctggtgcatg aagtcctagc
     6661 aaacgaaggt cttctttcaa taatacagtc actgcataaa ctagagctaa gcgtgcttct
     6721 ttttgttcat catcagctaa aatcttcgta tgagcatagt atttgttgaa tgcttgtgct
     6781 aatttgattg catgtttcgc aatcactgac ggttcgtatt tttccccagc agacaagacc
     6841 gtttcagggt atttttgaac taatttaacc acttcccagc tggtatcgtc attcaatgca
     6901 tagtctgctg tttctgaagg gacaaatcct gctttttcca acaggctgac tgcacgagca
     6961 tgtgtatatt gcacataagg tccagtttct ccttcaaatc gaacaacttc ttccaggtta
     7021 aagtcaaatg tattcagtcg atcgtttttc aaatcgtgga aaatgactgc cccaacccca
     7081 acttgtttag ctacagcatc tttgttttcc aaatcagggt ttttctcgct gatttgttct
     7141 ttagcagatt ggattgcttc atttaatact tcttctaata agacgatctt tcctttacga
     7201 gttgataatt ttttaccacc ttgcgtgatc aaaccaaatg gaatatggtg catgtcgtca
     7261 gaccagtcaa agcccatttc cttcaagaca gctttcaact gtttaaaatg atagctttgt
     7321 tcattcccta ctacatatag agattgtttg aagtcgtaag tacgtttacg gtacaatgca
     7381 gcagctaagt cacgtgtaat gtatagtgtc gcaccgtctg acttcttaat caacgctgga
     7441 ttcaggtcat aagcagatag gtcaacgatc tctgcacctt tatcctcgtt cagcaaatgt
     7501 ttttcttcta aaagtttgac gatttcatcc atcttgtcat tgtagaaagc ttcaccgttc
     7561 aacgaatcga aacgtacttc cagcagatca tagattttat tgaattcttt catcgattca
     7621 tcacggaacc attgccataa ttgaatagcc tctttatcgc cttcttccag acgtttgaac
     7681 caagcacgag cttcttcatt taattctggt tctgtttcag ctacttcatg gaattggacg
     7741 tataaacgaa gcaattcatt gattggttct gctttaacag cttcttctgt accccatttt
     7801 ttgtaagcga cgatcagttt tccaaactgt gttccccagt cacctaaatg gttgatgcgg
     7861 attggctgat aaccgatttt ttccataatg aaaccgatcg aattaccgat aactgtcgaa
     7921 cgcaaatgtc ccattgagat tggtttggca atattaggag aagacatgtc gattggtact
     7981 gttccttgat tcccgatgtt gctatcccca taatgttctt tttctttcac tactgtttgc
     8041 aaaacttttt tgctgataag ttctttattc atgaaaaagt tcaagtaagg tccgacaact
     8101 tcgatttttt caaagttggc agaatcgatt ttttctgcta aatctgcagc gatttgctgt
     8161 ggcgcttttc ggtaaacttt tgctaatgaa aaggctggaa atgcaacatc tccgtgttca
     8221 gctgattttg gattttctag taattgctcg acttgttcca gtgttaaatc atctttgaca
     8281 gcgtcataga cagcttttgc gacaagatct ttattattca tctttattcc tcctaaagtg
     8341 ttcccttgat tgttaaacaa aaaaaatctc tagcgacggc atacttagta tgccatcact
     8401 agagacgaga tttcccgcgg taccactcca attgtcctaa aaaaggacca gctccatctg
     8461 atatcggtag acgaccgtct gtttaaaaca gaattctcca agtgcgcttc gttgtatctt
     8521 cttcattcgg cttccaccac ttccgaattc gctagccaag aatgatacaa gtactctctt
     8581 gttcaacaaa tcttagtctt attaaacttt cttgaattat atcagaaaaa aaaacgaaag
     8641 gctagtcgtt tcataaaaaa aa
//
LOCUS       DASHRQ010000089         8490 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.093, whole genome shotgun sequence.
ACCESSION   DASHRQ010000089 DASHRQ010000000
VERSION     DASHRQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.093"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..173)
                     /locus_tag="U2M88_002513"
     CDS             complement(<1..173)
                     /locus_tag="U2M88_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8374844.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="U2M88_002514"
     CDS             complement(593..1714)
                     /locus_tag="U2M88_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEM8374845.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="U2M88_002515"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="U2M88_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEM8374846.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="U2M88_002516"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="U2M88_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEM8374847.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="U2M88_002517"
     CDS             complement(4304..5215)
                     /locus_tag="U2M88_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM8374848.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="U2M88_002518"
     CDS             complement(5232..6239)
                     /locus_tag="U2M88_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEM8374849.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="U2M88_002519"
     CDS             complement(6236..8365)
                     /locus_tag="U2M88_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEM8374850.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8490)
                     /locus_tag="U2M88_002520"
     CDS             complement(8370..>8490)
                     /locus_tag="U2M88_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM8374851.1"
                     /translation="GQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2679 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg
//
LOCUS       DASHRQ010000090         8457 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.094, whole genome shotgun sequence.
ACCESSION   DASHRQ010000090 DASHRQ010000000
VERSION     DASHRQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.094"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            384..1823
                     /locus_tag="U2M88_002521"
     CDS             384..1823
                     /locus_tag="U2M88_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM8374852.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2118..2336
                     /locus_tag="U2M88_002522"
                     /pseudo
     CDS             2118..2336
                     /locus_tag="U2M88_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2585..2800
                     /locus_tag="U2M88_002523"
     CDS             2585..2800
                     /locus_tag="U2M88_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374853.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3017..3273)
                     /locus_tag="U2M88_002524"
                     /pseudo
     CDS             complement(3017..3273)
                     /locus_tag="U2M88_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3460..4371)
                     /locus_tag="U2M88_002525"
     CDS             complement(3460..4371)
                     /locus_tag="U2M88_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM8374854.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4390..5394)
                     /locus_tag="U2M88_002526"
     CDS             complement(4390..5394)
                     /locus_tag="U2M88_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEM8374855.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5391..7514)
                     /locus_tag="U2M88_002527"
     CDS             complement(5391..7514)
                     /locus_tag="U2M88_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEM8374856.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7519..8439)
                     /locus_tag="U2M88_002528"
     CDS             complement(7519..8439)
                     /locus_tag="U2M88_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM8374857.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2430 a         1720 c         1561 g         2746 t
ORIGIN      
        1 ttagtataat cctatggaat tgaatatcat agcaatgtat ttgaaaagca aattggaaaa
       61 caaataggtg caacaccata aatatgaaaa aattgggaaa aagtagaatg gaaatttgct
      121 tacgtctcac gagatagctc acactattaa tctgaagaac aatgaagtcg acttttagaa
      181 attgctagaa gaaagtaaag cgaattatca ctagttacta tagagtaagt tcaaataaag
      241 catctccaac ttacatggtt acatgaggat atcaaatcga aattactcaa gcttcctatg
      301 gctagagtcg cgagaagttg ttgggcagta gacataggtt atattgttct tagctacacg
      361 atcattagaa aggaaatcct attatgtata gttatcaaga agatgcaaaa gtccaaaaaa
      421 atcgctggtg gattttgatt gctatatcta tgtttacctt catgtccaca ttggatggca
      481 gtattgtcaa tattgcacta cctaccatct cagatgatat gggcatacca atgaatcaag
      541 cggaatgggt agtgtccgtt tatctgatga ttgtctgttc ttgtttgttg ctgtttggta
      601 aaattggcga tagccgtggg aaaatcaaag tatttcggat tggtacgctg atctttgtct
      661 tgggatcgct gttgtgtggg ttcaatcatt cgctaggtat tttattgttc gcacgggtgg
      721 tacaagccat tggtgctagt atgactatgg ctacaaatgc cggaatcatc acggaagtct
      781 ttcccataca tgaacgtggt cgggcattgg gagcaatagg tgcgtttgtt tcattaggct
      841 cgattgctgg accaggtctt ggcggcttga ttttgtccca attttcttgg gcgtacattt
      901 tttggatcaa tgtaccggtg ggaatcgtaa cgatgttgat tgctgaaaaa ttcttaccaa
      961 aagatatcac catgagcggc catacaatcg atggaggggg ctttgctgcc attgcggcta
     1021 cgattatcag cttttttgga gcaatctttt tggggcaaga aagtggcttt ttccatccag
     1081 tcccccttgt actgttcgta atttcagtga ttgcctttgt cagcttttta aaagttgaaa
     1141 agaaagcagc ctctccattg atttctttcg gtatttttaa gaacaagatt tttaccatga
     1201 gcttgattac agccttgttg attttttctt ccaacttctt tgtcaatgta gtgattcctt
     1261 tttatctgca aaatgctcgc gggttgagtc ctagctatgc tgggctgttg atgatggtct
     1321 ttcctttgat gatggtcgtc ggggcgcctt taagcggcta cctgaccgac cgggttggcc
     1381 cggagatttt ggtattagta ggcttgggct tattgtcggt aacccaggtc atgtatatgt
     1441 ttttgtcagt ggactctcct ttgtggctat acattgtcgc tactgccatc atgggtgccg
     1501 gcaatgcctt gttccagtcc cccaataata cgatggttat gagctcggtc actatcgaaa
     1561 acttgggact ggcaggctcg atgaattcct ttgcccgaaa tttggggatg gtcagcggga
     1621 tttcgctttc tacgacggtc ttatatcaag cgatgtctgc taagatgggc cagcaagtaa
     1681 ctaactatat cgcaaatcgg ccggatgtct ttttatatgg aatgaagatt acctttttag
     1741 gcagctttgt tttgtgtttt gttgcctttt tcctgactat ctggcgtatc aaaaaacaag
     1801 gaggtattca gaaaaatgtc tgaaaaaacc actgttgaag aatttttgga ggcctgcgta
     1861 tgggtgagtg ggatttccat ggatgatgga agatgaagtg aacttctaaa agccgaaaaa
     1921 ctcaagtatt atcttcaaaa tactagtgct caatgagatg cttgatttga tcacaagaag
     1981 actgcgaaag ccgctggcaa ccgcaagcta ggtatcccac cgatggacta cgttttagag
     2041 gctactggtt atgaatacgg agccaacgct ctcatctgca taagaataca tcacccagat
     2101 tatgtaaaga ttttcgatac ttctgtcaaa aaatcattaa gatagataaa tgatttccgt
     2161 ctagtcaaat tggttcagaa tgtagacaca cagatggtaa gaagtcactc acaattcgag
     2221 aatggacttg tcctatttgc cgtatacatc ataatcgaaa cgtcaacgat agtatcaata
     2281 ttttgaccaa gggctatgaa tacaagcatt gccttagaaa acatcaagaa ccttaggaac
     2341 aacggggata gcttggttaa taagagatac cgctgtttag gcttgcaagt atactctttt
     2401 atcaagaatt ccgtggcttt agtcatgaga ggcttaatac tatgaaaatt ttctttagca
     2461 tttttgattt aggttccttt tctaattata ataagatgac cactacagta acctactgaa
     2521 atatataaac agccactgta gtaccatttt caaagtagtc tatgagagga gaatcttcat
     2581 tcttatgaac gctggaattg cggatatgaa tttaataaaa aaaaccctta atgattttac
     2641 ttccaattca atatcaaaag gtactggtat caatttaagc acaataaaaa agctgaaaag
     2701 tggtgaaaga tcagtggaaa agctgaatct tttagatgct ataaaaataa cggaatttgc
     2761 tatgaaaaat ggaaaagctg agattgaaat atggagataa ttatcgtcta gtgaattact
     2821 tgccacttag ctgaatttaa cgatccttgg tgcttgaagg gggagattct tgggaataga
     2881 gcatacttgc aagcctatgt ctttactaac aacgatatct cttattaacc aagctatccc
     2941 cgtagttcta aggcttttac ttttatctaa gcaataatcg tattcaaagg aaatttttga
     3001 cccttagaaa gtctcatttt atcagacgtg agcttaggca ttgctgaatt ttgttaattc
     3061 ggttttaggt tgtgctttga gcataatatg aaagtattct ttatcacgat ttcattcatc
     3121 taaggtaatg tgatgtgatt ctgaaattct ttcaaaggca gtttttgcat aagctgacat
     3181 tacatcatca atgacatgtt tacaatgttt cactactaaa ataagggggt aaccaagaag
     3241 aaaaactgaa tgattatttg tatctaattc catggtatat caaccatttc ctatatagac
     3301 tgattatgac atggattaaa ataaaaaggc actatgtctt aattgtcggt tttcgagtta
     3361 ccgaatggca ttcatcaccc acctatagag gatgggcgac ttctgcctga aataagttac
     3421 actacttata acttctaata aaattagatt gagtcattat tatttaacat caacgatttt
     3481 ccagtttcca tttttttgta atactaattt gtactgaaag tagctagtgg tttttgatac
     3541 attatcaagg tattttatct ttgtactcac tagaaaattt tttccgtttt gtagataaac
     3601 tggattctct aactccgaaa actttaagtc aatattgatt ggtcgcatcg caccattttc
     3661 aacataatac tccaattctt tttcagaagc tgatggataa agtttaaaaa atgtttctaa
     3721 aaaatcggtg atttcttccg ttgttttggc atcaatttct gaatcgtttt ctacttgttt
     3781 tgcttcatat tttgccttgt ctggctttcc aatcattgtt ggcaaactag ttactaaatg
     3841 attcctgttt tcatcttcaa agattgtcac tcgatatgca gaagaaattg attgcgtctt
     3901 ttttccctct gtaatatgtt gctccactaa aaatgaaacg acaaattctt ccgagtgttt
     3961 ttcaacatct agaattttta cagattgaac ttcggaactc gtgggaatat ctgctcgaac
     4021 catatcttgg attaaagcga gaccgttttc tgtaaggtac tgttcgagat tactcattct
     4081 cttttcaatt gcttctttgt tatttttcca tgaaaaatat tctttcgcaa aatcctctgt
     4141 aaaactttca acgccagaag tattcactac cttctctttt ataaccactt tttcatgagt
     4201 tgtatgttga tcaatggcgg tgaaattttt ataaacgcca aaagcaagac tacaagacaa
     4261 taaaagccaa agggcaagca ccatttttcg atgcttaccc acggatacaa ctcgttcttt
     4321 tttctttttt gggctctttt ccttttgatt ccgttcaatt tttatcttca ttctttttcc
     4381 tcttttctct tatttgtatc ggccagctcc gataagatgc tgccgccaat agttttccgt
     4441 tagattcgtc caccccactg gatctcctgc atgaaacatg cgattctccc ctgcgtaaat
     4501 gcccacatgg gtcacataag taccggcatc ataggttgaa tgaaagaaaa ctaaatcacc
     4561 tggtttggca tcttttagat caatatgagt catggcatca tattgatcct gagccacccg
     4621 aggaagtttc aggtcaactt tggcataaca ccattgggtc aagccactac aatcaaaccc
     4681 cgtttctgga cttgagccac caaacacata ggccgttcct tcatacttaa atgcctcatc
     4741 aaaaatttct tggactattt tgtcatcaaa ctgagttgta gttaggtatt gcttcactaa
     4801 taaacagtaa aacatattgc cataattata ccgccaccca ccattttcct taatcgcgat
     4861 tgggttgggg taatcgactt tttgaccacc tgatttttcc tttgaaaaag attcagctaa
     4921 agcaaaacta tgcttcttcc cgtttttccc gacatgatct agatacccgc caccatagtt
     4981 ataggcctga ataatactat cttgatcaca ccctagttct ttggaacgct taaccaaaga
     5041 cgcaaaatag agacagcctt gtttgatgga tgcctcggta cttaatgaat taggtggtaa
     5101 tccggctgac tcagaacttt gcataacgtc ttccattgtg ccaccagatt ccacttgaat
     5161 aatcgccaaa agaattggaa tatactctgt tatatccgat tctttggcat acttttccac
     5221 cattgcacgg tgtttcaata cttcttctga gacactagtt ccgccaggag aagtgccaga
     5281 actaccattt ccctcactgt cttcaccaaa caacaaaccc acacacatca aaatacccat
     5341 aaaagagatg gtcagaaaag ttaatgaaat aacacccact ttcttcaagt tcattttttc
     5401 tcacctgacc ttgattttgg acgatggttc attctttttt gtactttttg attggtctgc
     5461 tttggcagag tggacctctg tttcacttgt tggaaagaat ttttcttact tgctgattgg
     5521 cttttccctt ttgaaatgat ttcttgacta gttagttttc gtttgatttc gggattcaga
     5581 tttgctctca caatccgctt tggtgcttct tttttcagca tcatatcttt ctgaggttga
     5641 gctgctttat ctattggaat attcgccctt ctttttccct gacgatttgt tgcttcactc
     5701 agtatcttgc gtttttcgtc cattttcgca tttcttcgtt gtcgttgttt ttctcgcaaa
     5761 tccactcgat ccgctttttc ttggaacagt ccgcgcttga attcttctgg tgcttttttc
     5821 gacttttcga ttcctttatg gagattgtat ttcaaattcg ttggcgtatt ccttacctga
     5881 tcctttactt gtttagtctt atctttcacc aaagcttggg tatccaaaac tttgccgatt
     5941 tttctcccag ccaaattcat tcgggattgt ttcttattat ttgaaactgg attctcctta
     6001 gttaagtcag atagtatttc gtgatcgttc ggtaatcgtg aattctttcg tagagaagaa
     6061 ccggatggct taaatttgga ttttgattgt tcctttgcca acttatttcc gactgctgca
     6121 ccaccaccaa aagccaaagc tcgtcccaca tttcgattga gttgccgcaa ttttcgattc
     6181 attaacattt gcggttttcg catgacgcgt cttcccattg actggctatc attactctgg
     6241 aggttgaaca tactcataat gtcgcccaat ttgaaataga taccggcaaa ggtcacaatt
     6301 tgcagaaaag caaccatgaa aaatggatag gttgccgaaa tattgaaaaa catcgtggag
     6361 atgctaaatg cagtcgtaat caccaaagta acacccgctc gcatcataat cgtattgaat
     6421 aaacgaataa tcgccttctt tcctaaactt tcataagtcg gcaacatgga caataagaaa
     6481 ctaattggca gaaacatcgc aaagataatg aagagaattt gcgagaacaa cataatccct
     6541 gttagaagaa aaacaaagaa ggaaatgcca atattgaaca ataccaaaaa gaccacggtc
     6601 cctaaacggt tcatggtctt ggtaatactg agattcgcat tgtcattgtc ttcaatctct
     6661 gccttgaccg cttcttcccg atctttgcct ttgttttcat ctggactcac agatagaatc
     6721 ttgttgaccc ggtcttcacc aatttcttct atgttggaat catcaaattg cagtagtaac
     6781 caaggttgtt ggacttgaat cgaaaacaaa ctatcccgaa tcaaatccac actatctttt
     6841 ccttggcttt ctgaattggg tacaacgatc tcggttccaa gggtaagggc cgcttcacta
     6901 acatctgagc taaagtcatt gatttttgtg atataggtag gcgcataagc aatgaacgaa
     6961 cccgatagta gaaaaatcat cacaaaatta atcacggctc gaactgcttt cgtggtttct
     7021 cgttttaata atccggtata cgccacatac acccccagag ctaaaatcat caataataga
     7081 aacccaaaat aaaatccgga ggtctggagt ccattttcag taataccagc caatgtttgg
     7141 atattctttc caatactttc agccgtatcc gaaatgaaat ctaatttgta agcctcttga
     7201 accacataac ccgtggcgtt cgacaaatac aaactcaccg tccaaatgaa attagtaatg
     7261 gcataaagac catacatcac gcttttccct aagccatcac cccaattcca aggcaaccaa
     7321 tcccaagagc tatccacaaa gaaatctagt tgatagttgt tcagtgaata tttggaatat
     7381 agatttcctg cttcaactgt atcatcaact agccctgtcg cctcggctac cgtaccaact
     7441 aaactcaata gaactatcag agtgagtatg ctaataaaaa cgattcccac aatccgaaaa
     7501 agtttctttt tcatcccctc actccattct ctcttgaaca ggtggacggg tatcaaaggc
     7561 atggaagagt tcttcaaaga ctggatgtac ctgaaccacg cctacccgac catacaaatc
     7621 ctgcatcaag cattggccat tttccaaatc tcgtaggcgt ttttgattgc tttcatcttc
     7681 tcgatccaag ccaaagaatt ccaaggtttt cttgatttca tgaatatcgg tactgcgaaa
     7741 cgcaaacttc aacccgatat tattcttcaa gcgttcatcc agtaaatcat cactattttg
     7801 cgtgacaaag taaaccccgg cattcatagc ccgcccagca cgaacgagct tattcgataa
     7861 tgttttccct tgcgctactt gaagaaaact ccacgcctca tctaagtcta caattttgaa
     7921 gatttctcga ttactatgga taaagtccaa ggcaaaagtg gaaatgacga ttagcatgga
     7981 aacactaagc aattccatgg tggtatattc ttcaaaagtg gtttctgcat ctggtaaaac
     8041 caaatccgct acttgaatga tatttaactg cctatctaaa ctaatcgact gggtaatcga
     8101 accatctgag aaaagcaaat gggcaaaatc ataatcggta aaggactcga tgtgatccgc
     8161 gatattttcc gcaactggtg agccttcttt tcgaagctca ttaattactt gtaataatcc
     8221 ccgttgattg ctttgggtaa ccgtccgaat ggccttacgc aacactggaa acttctcccc
     8281 atctcgacta gaaattcctg ttaaaaaggt cagaatatca attgctagac tttcagaatc
     8341 cttctttttc tccataatca cataaggatc aagtaatcct tgattttttg gcttactagt
     8401 taagttgaga atattgattt catccgcaat ttctggcaag gtttctttcc agccacc
//
LOCUS       DASHRQ010000091         8298 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.095, whole genome shotgun sequence.
ACCESSION   DASHRQ010000091 DASHRQ010000000
VERSION     DASHRQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.095"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            47..679
                     /locus_tag="U2M88_002529"
     CDS             47..679
                     /locus_tag="U2M88_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEM8374858.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(681..1586)
                     /locus_tag="U2M88_002530"
     CDS             complement(681..1586)
                     /locus_tag="U2M88_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEM8374859.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(1671..2330)
                     /locus_tag="U2M88_002531"
     CDS             complement(1671..2330)
                     /locus_tag="U2M88_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEM8374860.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            2646..3590
                     /gene="mvk"
                     /locus_tag="U2M88_002532"
     CDS             2646..3590
                     /gene="mvk"
                     /locus_tag="U2M88_002532"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEM8374861.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            3583..4560
                     /gene="mvaD"
                     /locus_tag="U2M88_002533"
     CDS             3583..4560
                     /gene="mvaD"
                     /locus_tag="U2M88_002533"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEM8374862.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            4573..5658
                     /locus_tag="U2M88_002534"
     CDS             4573..5658
                     /locus_tag="U2M88_002534"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEM8374863.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            5655..6719
                     /locus_tag="U2M88_002535"
     CDS             5655..6719
                     /locus_tag="U2M88_002535"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEM8374864.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(6968..7771)
                     /locus_tag="U2M88_002536"
     CDS             complement(6968..7771)
                     /locus_tag="U2M88_002536"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEM8374865.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(7791..8096)
                     /gene="kduI"
                     /locus_tag="U2M88_002537"
                     /pseudo
     CDS             complement(7791..8096)
                     /gene="kduI"
                     /locus_tag="U2M88_002537"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(8078..>8298)
                     /locus_tag="U2M88_002538"
     CDS             complement(8078..>8298)
                     /locus_tag="U2M88_002538"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEM8374866.1"
                     /translation="ALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLIVI
                     IIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         2505 a         1740 c         1625 g         2428 t
ORIGIN      
        1 gattcccgct tctttgctat actaaagaaa aacgaggtga acccacatga aaaaaatcga
       61 acctattcca ccttctcttc ctcttcttac aaagggagag ttttatttag aagatgagga
      121 gatcttctct ggtcttctga tcaacggagg taaccaaagc tatctcgatg ccaaaaatat
      181 ggtattaaga gagagtcata tccaaaaact tacgatgcaa aaaacaggtt tacgtcattt
      241 tgaatgcagt aatgtcattt ttgaaaaatg tgatttttcc aatctggagt ggattggtgg
      301 cagtttccac caagtcgtgt ttcatcaatg taaattaaca ggtacgaatt ttgccgaaag
      361 ttatcttcga gactgtactt tcactgattg tattgcttcg atggcttcct ttagtagtac
      421 aaacttgaaa gctgtctcct tcgatcattg tcaattagaa gacagcgaat tttatgaggt
      481 cacttggaaa aacctttttt tgtctgacaa tcagttaacc ggttcgaact ggtttcgaac
      541 ttctctgaag ggattagact ttacgactaa tttcttcaca acaatcgctt tatctcaaga
      601 ttctttggcg ggactaatcg tcaatcaaga gcaagcactt gtgatcgccc aagcccttgg
      661 tttgcagatc aaagactaga tcaacgaatc cgccgccaca gtgcaataag catccaaata
      721 gataagacca aactgatgat caaggcgatt tcccatccga aaacggaatg ggtaaagggt
      781 aactggagat tcattccgaa gaatcctgtc acaatagttg gaacggtcaa gatcagcgac
      841 cagaccgtca aaaatttcat cgtgtcattc aagttgttat tcagcaaatt attataagtg
      901 ccagagagct ggtctaaaat ctgcgaagct aagttcgtca tttcaactgc ttgtcgtgct
      961 tcgatcagtg catcgtccaa ctcttctttt tccttttctg acaacttgcg ataaatagcc
     1021 agtgccttga tttgttctaa aagtactaca ttttgttttg ttcctgtaac taagtaaacc
     1081 aaaccgatct ctaaatctga aagttgcagc agattcttgt tcgttgtttt ttctcttaat
     1141 ttcagattaa gccgctgacg ttcactattc acttcctcga ttaaaggaaa aaaggaatca
     1201 gaaatcaaaa acaatgtacg aaacaataaa ctgtatacac tcatgtctgg catccgttcc
     1261 aaaagcgatc gaattagtcg aatcacgtac tcggtattcg gcgtggtaaa tgtaaacaaa
     1321 ccgtcttctt ttaaaataaa agcgatagga ctagtttcat aatgattatc gactttttgc
     1381 tggtgcggaa catttgatac aagcagtaac gcttcatcaa aagaatcgta ctctacgcgc
     1441 gcccgttcat ttttatctag agaataagtc agcatctcgt cactaatgcc atattcttct
     1501 tttaagtttt taacagctag cacatcgttt tgctgtacat tgatccaaaa attacggtct
     1561 tgattaaaat agtgtttttc ccgcatactc tatcctcctg acatttgttt ccagtataaa
     1621 gaaaaaagtc gctcaaataa aggtttaccc tttatttgag caactgtaaa tcaaatggat
     1681 actttggttt ggcgaatctc tgctaatttt tcttcgatca ttttcaagac ttcttctttt
     1741 gcttgaggat cttccacaaa attatagcgg tctccatcga tttggatttt tggactttct
     1801 ttgtattctt cataccattg atcataacgt ctgttcaatt ctttgtaata atcgtataga
     1861 cttgggtcaa aggacaattg ttcatacgaa cgaccacgtt tttcgatacg ctctaacatc
     1921 gtatcaaaag atacacgaat atgaactaat aaatccggat gttttttatg ggcagcatag
     1981 ggaagttctt ccatcatgtt ttccagcagt tcatcataca cacaaacttc tgtttctgtt
     2041 gctcttccta gatctgcatt tagatggaaa agcagtgaat cttcataaat cgaacggtcc
     2101 aatacattat tgtcatcttt cattgcttgt ttgatgcttg caaaacgttt attcaaaaaa
     2161 taaatttgca gcaaaaaagc atactgttca gggtttgcat aaaataaggg caagacttca
     2221 ttatcatcaa tcgattcata aaatgcctga ctatcaaagt gttcagccat catttctgtt
     2281 aaacttgatt ttccggcacc aattgtacct gctaaaacga tcacgctcat ttacctccag
     2341 ctcacttcat cactaaatat tgtgtttaac tccaaccttt tttaaatata cccccaatat
     2401 ctagtagtgt caattgtatt ttttctagtc aaagaagaaa aaaagtcaaa aaattcttaa
     2461 gaaaaaagtg ttttttcttt agaaaacgta ttcttagttt tacttctgag atggttttag
     2521 taaaataaag gaggaatgtt ttttgagacc cttagtggac aaaaaaattc gtgaagcagc
     2581 gcaactcgtt ttgggcgctt tattttttgc ttaaaaatca cttttacgtg agaaggagtt
     2641 agattatggc aaactatggc caaggagaat caagcggaaa gatcatattg atgggcgagc
     2701 acgcggttgt ttatggagaa ccagcgattg cctttccttt ctatgcaaca aaagtcaccg
     2761 cattccttga agagctggat gcaatggacg atcaactggt ttcttcctac tattcaggaa
     2821 atttagccga agctcctcat gcattaaaaa atatcaaaaa attattcatt cacttaaaaa
     2881 aacagcatga catccaaaaa aacttgcaac tgaccattga aagcacgatt cctgctgaac
     2941 gtggaatggg atcaagcgct gcagtcgcca cagcagtcac tcgtgctttt tatgattact
     3001 tagcatttcc tttgtctcgt gaaatactat tagaaaatgt ccagctttcg gaaaaaatcg
     3061 cccacggtaa tcctagtgga atcgatgcag ccgctactag cagcttgcag ccgatttatt
     3121 ttacaaaagg gcatcctttt gactactttt ctttgaacat cgatgctttt ttgattgtcg
     3181 ctgatacagg aatcaaagga caaacaagag aagccgtcaa agatgtcgct cacctctttg
     3241 aaacccagcc tcatgaaact ggacaaatga tccaaaaatt aggatacttg acgaagcaag
     3301 caaaacaagc aatcattgag aattcaccag aaacgttggc acagacaatg gatgaatcac
     3361 agtcacttct ggaaaagctg acaatcagca atgattttct taatctattg atccaaacag
     3421 caaaagatac cggggcattg ggtgccaaat taactggcgg tggacgcggt ggctgtatga
     3481 ttgcattagc acaaacaaaa acaaaagccc aagaaatcag tcaagcactt gaagatgcgg
     3541 gtgctgctga aacttggata caaggattag gagtacatac ctatgtttaa aggcaaagca
     3601 cgcgcatata ccaacattgc tctaatcaaa tattggggta agaaaaatga agaactcatc
     3661 cttccaatga acaacagcct ttcgttaaca ttggatgcat tttatacaga gacagaagtc
     3721 atcttttctg atagctatat ggtagatgaa ttttatctag atggcacctt acaagacgaa
     3781 aaagcaacaa aaaaagtcag tcagtttctt gacctttttc gtaaagaagc cggcctctca
     3841 ctaaaagctt cagtaatcag tcagaatttc gttcctaccg cagctggatt agcctcctct
     3901 gccagcgggc tagctgcttt agcaggagct tgcaatactg ctcttaagct tggattagac
     3961 gatctctctc tttcaagatt tgctcgacgc gggtctggtt cagcttgccg aagtattttc
     4021 ggtggtttcg ttgaatggga aaaaggccat gacgacttaa gttcttacgc taagccagtc
     4081 ccttccgatt ctttcgaaga cgatttagca atggttttcg ttttgatcaa cgaccagaaa
     4141 aaagaagtgt ccagcagaaa tgggatgcgt cgaacagtcg aaacatccag tttttatcaa
     4201 ggctggttag attccgttga aggggatcta tatcaattga aacaagcaat caaaacaaaa
     4261 gatttccaac ttctcggaga aacgatggaa agaaacggac taaaaatgca tggaacgact
     4321 ttagctgccc agcctccatt cacttactgg tctcctgaca gcttaaaagc gatggatgcg
     4381 gtgcggcaat tgagaaaaaa aggcattcca tgctacttta cgatggatgc tggccctaat
     4441 gtcaaagttt tagtggaaaa cagccattta tctgaggtac aagaaacatt taccaaatta
     4501 tttagcaaag aacaagtaat tactgcccat gctggtccag gaattgcgat tattgaataa
     4561 agggagattt ttatgattga agtatctgca ccaggcaaac tctatattgc cggagaatat
     4621 gcagttgttg aaacaggaca tccagcagtt atcgctgcag tcgatcaatt cgtaacagtc
     4681 actgtagaat ccgcacgaaa agtcggaagt atccaatctg cacaatatag tgggatgcct
     4741 gtacgttgga caagacgcaa cggagaatta gttttagata ttcgggaaaa tccttttcat
     4801 tatatccttg ctgctattcg tttgactgaa aagtatgcac aagaaaaaaa cattctttta
     4861 tccttttatg atctgaaagt aacaagtgag ttagacagtt caaatggccg gaaatatgga
     4921 ttgggttcta gcggtgccgt gaccgttgca acggtcaaag ccttgaatgt tttttacgcg
     4981 ttgaatttat ctcaattgga gattttcaag attgcagcac tagccaattt agcagttcaa
     5041 gataatggtt cctgcggcga catcgctgct agctgttatg gtggctggat cgctttctca
     5101 accttcgacc atccttggct ccaagaacaa gaaactcagc attctatcag tgagttactt
     5161 gccctggatt ggccaggtct atccattgag ccattgattg ctcctgaaga tttacgttta
     5221 ttgattggtt ggacgggtag ccctgcctct acttctgatt tggtcgatca agttcaccgt
     5281 tcgagagaag acaaaatggt ggcttatcag cttttcttaa aaaacagtac agaatgtgtc
     5341 aacgaaatga tcaaagggtt taaagaaaat aatgtaacgt tgatccaaca gatgattcga
     5401 aaaaaccgac aattactgca tgatttatct gcaatcactg gggtcgtcat cgaaacgcct
     5461 gctttgaaca aattgtgtaa tttagctgaa cagtatgaag gagccgcaaa atcttctggt
     5521 gcaggtggtg gcgattgcgg aatcgtaatt gttgaccaga aatctggcat ccttccttta
     5581 atgagtgcat gggaaaaagc agaaatcact ccactgccgt tacatgtcta tagcgatcaa
     5641 agaaaggaaa accgatgaat cgaaaagacg aacatgtttc actagctaaa gcctttcatg
     5701 ataaacaaaa aaacgaattt gacttcgtac ggatcatcca taaccctttg ccgcaaatag
     5761 cagtttctga tgtcgatcta agtacacagg cagttgggtt tacactaagc agcccatttt
     5821 atatcaatgc gatgactggc ggaagcgaaa aaacaaaaaa aatcaaccaa gatcttgcaa
     5881 tcgtcgcaag agaagcagat ttgatgattg ccactggttc agtcagcgct gccttgaaag
     5941 acccttcttt agccgatact tatacgatta tgcgacaaga atatcctcac gggaaaatca
     6001 ttgccaacat cggtgcaggg acttctgtcg aaagagccca agaagctatc cgattgtttc
     6061 acgcagatgc cttacagatt catttgaatg cacctcaaga gttggttatg cctgagggag
     6121 accgtgattt taccaattgg aaagtactta ttcaagagac tcaaacagcc attgacgtcc
     6181 ccctcatcgt aaaagaagtc ggttttggca tgacaagaga aacgctcaat gatctagctg
     6241 ctttaggagt tcatacagtt gatatcagcg gtcgaagcgg aacaagcttc acccagatcg
     6301 aaaacgcccg tcgctccaag cgggaactga gttatttagc tgactggggg caatcgacag
     6361 tctcttcatt gcttgaagca aatgaagcag acacttctat ggaaatccta gcctccggag
     6421 gtatccgcaa tgcctatgac atatttaaag ccctttgtct cggcgcaaat gcagtgggta
     6481 cttcaggaac cgtactgacg catctgatga atcacggcgt agaagaaact attatactga
     6541 tgaagcaatg gcaagaagag cttcgtctgc tttatactat ggtcggcgca acgaataccg
     6601 cggccctaca ccaacaatca ctgatctttt ccggaccagt gaaggattgg tgcgaagcaa
     6661 gagggatcga cttagtgaag tatggaagac gcacagaaaa gagcgtggga caaaagtaaa
     6721 gaacactttt atcccacgct ctaaacacgt ataaacggcg gaagcaaaag caactccttc
     6781 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattc cttcttattt
     6841 cttggagtta aacgcttctg tcccagcctc ttttttcgaa taaatgatgt ttaatcagct
     6901 gtccttcggt attcgttcaa gccgctgatt tcacaacctc tccactaaac ggtgtactaa
     6961 agctctttta aggttgtttt ccgatatagg caagtatgcc tccgtccaca tacaagacat
     7021 ggccgttaac gaaatcagat gcgctagaag ccaagaagac agatggtcca gccaaatcga
     7081 ttggctcgcc ccagcgtgct gctggcgtac gtccaacgat aaattgatcg aatggatgac
     7141 gttcaccgtt ttcttgtgtt tcgcgtaatg gagcagtttg cggtgtagca atataacctg
     7201 gtccaattcc attacattgg atattgtatt gaccgtattc agaagcgatg tttttcgtca
     7261 gcatttttaa tccgcctttt gccgcagcat aagcactgac cgtttcgcgt cctaattcac
     7321 tcatcatgga gcagatattg atgattttac cgccgccttt ttcgatcata tcccgaataa
     7381 ctgcttttga aacgatgaat ggggcattta aatccacgtc gattacttgg cggaagtctg
     7441 ccgcatccat ttcgatcatc ggtgtccgtt tgatgatccc tgcattgttg actagaatat
     7501 caactgaacc tacatcttct ttgatctgtt gaaccatcgc ttgaaccgct tcttcatcgg
     7561 tcacatcgca gacataacct tttgcatcga tgcctgcttc tttataatgc gctatacctt
     7621 catcaactga ttcttgattc aagttattaa atacgatttt tgctcctgct gtagctaaag
     7681 agcgtgcaat ctcaaatcca ataccataga cagcaccggt gactaatgct acttttccat
     7741 ctaaacgaaa catatccata ctaaattcca tcattttctc tcccttatgc ttattttaat
     7801 tgatccattt gcaccatatc catgtctgta taggtgatat tttctccgca cattgcccag
     7861 atgaatgagt aattgcttgt acctacacct gaatggatcg accagcttgg cgaaataatg
     7921 gcttgttcat tgtctacgac taaatgtttt gtttcatctg gtttgcccat catatggaac
     7981 acacgtgtat cttcattggc ataatcaaaa tagacataag cttccatacg acgttcatgt
     8041 gtatgacaag gcatcgtatt ccaagcactt cctggttcta aaatcgtata acccatttat
     8101 aaatgacagc tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga
     8161 taatgatgac aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag
     8221 ccatctgtcc tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta
     8281 gcatgattcc tagcgccc
//
LOCUS       DASHRQ010000092         8066 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.096, whole genome shotgun sequence.
ACCESSION   DASHRQ010000092 DASHRQ010000000
VERSION     DASHRQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.096"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..882
                     /locus_tag="U2M88_002539"
     CDS             <1..882
                     /locus_tag="U2M88_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEM8374867.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            930..3059
                     /locus_tag="U2M88_002540"
     CDS             930..3059
                     /locus_tag="U2M88_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEM8374868.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3034..3723
                     /locus_tag="U2M88_002541"
     CDS             3034..3723
                     /locus_tag="U2M88_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374869.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4131..4817)
                     /locus_tag="U2M88_002542"
     CDS             complement(4131..4817)
                     /locus_tag="U2M88_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM8374870.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4953..5147)
                     /locus_tag="U2M88_002543"
     CDS             complement(4953..5147)
                     /locus_tag="U2M88_002543"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374871.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5197..5637)
                     /locus_tag="U2M88_002544"
     CDS             complement(5197..5637)
                     /locus_tag="U2M88_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEM8374872.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5641..6486)
                     /locus_tag="U2M88_002545"
     CDS             complement(5641..6486)
                     /locus_tag="U2M88_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEM8374873.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6635..8053
                     /gene="pepV"
                     /locus_tag="U2M88_002546"
     CDS             6635..8053
                     /gene="pepV"
                     /locus_tag="U2M88_002546"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEM8374874.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2481 a         1515 c         1713 g         2357 t
ORIGIN      
        1 atttcagtac cggttgcact gttctcattt atgggctcga ttatggctgg aatctttgct
       61 aatatcgtca atttcaaaca atatcaaacg atttatgctg gtttcaatcc agcggctgaa
      121 agctatactt ataatgacta tttccaaatt ggtatgattg ggatgattgt aagcttagtt
      181 gtggtactaa cggtcgctaa tatatcaatg aacaaaaaga aacgctatgc aatggcagca
      241 aacgtaccag ctgaaggtgg cgacgcacca atgatctcat ggttagccgt attattacca
      301 gttttagggg tcgttctttt agatcttcaa atcatcttag gttttattct agcaggtatc
      361 tgggcattgc tgtttactgg aaaattgcgc ggaggttata aagaaatctg tcgtcagttt
      421 gcgaaattat ttacagatgg cgcagtcgat gttgcaccaa tggttggttt cttgatgacc
      481 ttagcgatgt tcaataattc agctgcgtat gcttctcctt atttttcagc tatttttgga
      541 gattggatac cgcaatcacc attagtcttg gcaatcgtat ttgctatatt gacaccacta
      601 ggatttttcc gcggtccaat gaatctagtc ggttcaggct cagctatttt agctgtcgtg
      661 ttagctgtta atccaacgat gtcgccagcc tttttgttcc cgttatttgc aattacaacg
      721 attgctcctc agcatttaga tattacacaa tcttgggttg cttggggatt aggttataca
      781 aaagtaactt ctcgagaata tatgaagaaa tccatcccaa ctggttggat catcggtgcg
      841 atcttatgcc tgatcacgtt cttattgtac gggaacgcct agtaaacaaa acgtcgataa
      901 aaacgattag ataaatggag gaaaagaaaa tgaaacgttt tgtgcgaatg ggaattgatg
      961 ttggcggtac acatacgaaa gcagtcgcaa tcgataatgc gacgcacgaa atcatcggaa
     1021 aatcttcagt gaaaacaacc cacgatgatg tccgtggagt agctgcaggt gtcgttcagt
     1081 cttttcaaaa ttgtttgcgg gaaaataata tcagtccaga agatgttgtg tttgttgcac
     1141 attctaccac acaagcgact aatgcactga ttgaaggaga cgtagcaaaa gtcggcgtaa
     1201 tcgggatggc aaaaggtgga ttagaaggtt ttttagcaaa acgacaaact agattgaacg
     1261 atatcgattt agggaataag aaaaaaatag agattgtcaa tgcattctta cctgtcaaaa
     1321 acttaaatgt agaccgtgtt tcagaaacga tttcttcttt agaaagagaa agagcggagg
     1381 tcttggtttc ttctatggca tttggagtag ataatgggga accagaacga gttgtttatg
     1441 aagcagcatc tgtgaaagcg atccctacaa cgatggcttc tgacatcaca aaattatatg
     1501 gattgacaag aagaactcgg acagcagcaa tcaacgcatc tattttacca aaaatgctag
     1561 atacggcaac ctcgacagaa gactccgtac gagaagcagg ggtcaatgtt tctttgatga
     1621 ttatgcgcgg agacggcgga gtaatggaaa tcaatgaaat gaaaaaacga cctgttttaa
     1681 ccatgctttc cggtcctgct gcatcagtca tgggctcttt gatgtacttg agagcgtcta
     1741 atggtgttta ttttgaagta ggcggaacaa caaccaatat cggggtcatt aaaaatggac
     1801 gtccagcaat cgattattca attgtaggtg gccacccaac atatatctct tcattagatg
     1861 ttcgggttct aggtgtggca ggcggttcga tggtacgggc taatcagtca ggaatcatcg
     1921 atgtcggtcc acgttctgca catattgccg gtctagatta tgcagtattt acggagacag
     1981 aaaaaatcaa aggtcctaag gttgaatttt tctctcctaa agaaggagac cctgctgatt
     2041 acgtcaaggt agtgatggaa gacggagaag aagtgactat tacaaataca tgtgcagcca
     2101 atgttcttgg attagtgcaa gaagaacatt tttcttatgg aaatgtccca tctgcacgaa
     2161 aagccataca ggcattagca gactattgtc atacgacggt tgaagacatc gcagagcaga
     2221 tcatggaaaa atcctatgct aagattgaac cggtgatttt agaattggca gacaagtatc
     2281 acttggagaa agatcagatt tctcttgtag gtgtaggggg cggcgctgcc tcattgatta
     2341 cttactttag taataaaatg ggtgtgaaat attcgattcc tgaaaacgcc gaagtgattt
     2401 cttctattgg tgtagcactt gcaatggtcc gagatgttgt agaacgtatc atcccttcgc
     2461 caagtaaaga agatatacga tcgttgaaaa atgaagcgat gaataaagcg attgaatcag
     2521 gcgctacacc agaatcgatc gaagtccacg tagaaatcga tccacatact tcaaaagtaa
     2581 cggctatcgc tactggttct acggaagtga aagcaaccga tctaaccaag gagatcacaa
     2641 cagaagaagc actagagctt gctgcagagg atatgcgatt aaataaaaat gaagtttgct
     2701 tattggaaaa tacacctttc ttttatgtat gcggcgaaca gaatcgttca aaaaatgctg
     2761 gaagtcttcg gatcatcgat caaaaaggat ttatcaaggt ccaacgtgga catgcttcat
     2821 gtatgaaaac aactgcagcc aattatatga cagctgtcga acaactttgg gaagatatgg
     2881 cagtttatca aactgaattg attgcccgac cagaatttta tctctgctta ggagcaagag
     2941 tctctgattt tacagcgacg gatctggaac aactacagct tttgatggat ctggaagtat
     3001 ctactatgga accggaggaa gaagtgatcg tcgttgccgg aaatatcaaa caaacataag
     3061 ttagaagagt tactaaacga attacaacag atccccgaag aaatttgggg attctaccag
     3121 tttcggcgag atttattttg gaaaaagatt cctttgtcca agcagaaaat tcttatacaa
     3181 caatcaatcg attgtggtat cgaaacggca tgtagcatca aaaaaaagta cccttttgcc
     3241 gatgtaggag aaatctgtga acaaatggct gttccaatcg tttcctgtga atcagagcaa
     3301 ataaacgaac gaatcacttt tgcaacttat gcagaagatg aagggatccg attgatgacg
     3361 gagccgttag aaaaattaaa atgttcagga ctagcttcga tctctaaaga aacagcgcaa
     3421 gctttgatta ttggacatga actatttcac cacatcgaag cttctgttaa gggaatttat
     3481 acgcaaagcg aaaaaattgt gctgtggcgg ttgccttttt atacacatca atcaaacatt
     3541 cgtgcattga gcgagatagc agcgatgagt ttttcaaaag agatgaatca aagcgatttt
     3601 tcgccttatg tattagaggc agtcttactt tggccatata atgaaattcg tagccaaggg
     3661 atcttagaag aaataaaaga aatagaaaaa agatgtacgg agtatgattt agattacaaa
     3721 taaagagact gtgacaaaag tcgtgtcaca gtcctttttt tccgaataaa cggtgttctc
     3781 ctgtcaactc ctcggtcaca agtcaaaata ttcaacaaac atgagaagct gttttttgaa
     3841 attgttcctt gtgactcgga gatgaacggc aggctcacga cctcttggaa aaaggtgttc
     3901 aaaagcttgc atctgcggta ataagaacag ccaagttaaa aacataagaa gctattttta
     3961 cttggctgtt cttattactt gatgcagggc agctttttca caacctctca ataaacggtg
     4021 tgcaatcagc tgaccctcgg tattagctca aaaaaaacgg caaaatatga ggaactattt
     4081 tgccgtttat ttctctaata ctcaggtcaa aacgctgatt ccaaaacctc ttatttgatg
     4141 ggtctgtcct gaatatcttc aaaataatcg gggtaattat gaatcacgta ttcttgctcc
     4201 tcgataatca tatgtaaatg catcgtgcaa agaaatccga cttcatcaaa gttatgatca
     4261 atcattgatt gaagcattgt ctgatgttca tctaatactc gaccccagtc taattctgaa
     4321 atcgtcaaac gtaaccatct gaaacggttc aagtgcgtac tataactttc tagccaatcc
     4381 caaatttctc gttttccagc aatttcataa caagttcggt gaaaatcagt atccaaatca
     4441 aaaaaagctt ttttattttg agcgatcact gcctgatcgg tttgttctag tattttttct
     4501 agtttagctt gatcttgctt ggtcattttc gcactgcatt cttgcaggat cggtttttcc
     4561 aacttttccc gcgtataacg ggctaatgaa gcagatcgga tatcgatttt tgaaacataa
     4621 gtgccggact gtggaatggt ttttacaagc tcttgattgc gcagttggat cagtgcttct
     4681 cgaatcggtg tacgcccgat agctagttgt tcttctaaca ctttatcgga gattttactt
     4741 ccgggtgcta actcggaata aatgatttgt ttacgtatag attcataagc ttgctgttgc
     4801 aaattattga taaccattta accttccttt ctatgcagat ataatctatt ataacataaa
     4861 tcgcttacaa atcgtgtggg cataaaaaca cagtctgcaa aaaaagccct gataggcatg
     4921 cctattagag cctcgaaaag aaaaatattg aatcaatctg gtcgtacctc ttggatcggt
     4981 ttgacacgac cgccgattct tttagcaaaa gcttcggatt cttttttgtc tttgaaaatg
     5041 agcagattgt ctaagttttt ttcccgagtg cgacagccag ttttatcgat gtaaccgtgc
     5101 attactttaa cgacatacat ggggatcacc tgcttttttc ttttcattat acgaactact
     5161 cgacacaata caagaaaaat gcttagacgg tctttattaa gaatttacat catccaaaga
     5221 agcgtacgct tggaataact gttctaactc cggattgact ttgctcagct tcactaatcg
     5281 ttgcgaatca gaaagtaaaa aacagtgttt actactgcca gtagttttta attttttgtc
     5341 ttcagttccc acaatttcgt agctaaaatc caaccgtgtt cccgtatact tttggatagt
     5401 tgggtggatg ctcacttttt ctccgtaatg gatcatttct ttatagctgc aatttacttc
     5461 cagaacgggg atgatgatcc cagattcttc gattttttta tagggaaacc ctagatgtac
     5521 caataaggct acccgagcct cttcaaacca gcggatataa ttgctgtgat gaataatccc
     5581 catttgatct gtttcataat aaaaaggttc tcgcacataa cctgtaaatt tctccatgac
     5641 tcatgcctcg ttttccattg attttttaca aaaataattc atccacttcg gaagtgcttc
     5701 gctgatttgt gtcggcaaaa caatatattg ttcttttgcc agctggtctc caatgaaact
     5761 atgaagataa acagctgctg caatcgtttg gtcattttga ggaaactggg ctaaaaatcc
     5821 tgtgatcatc ccagctaaag tgtcacccat gccgcctgtt gccatagcag cagaacctaa
     5881 aggattggtg tagaagtgct ggtgaccata aatttttgtc tcatgacttt tcaaaacgag
     5941 cacagcaccg atttcctgtt gcttcttttt gttttcttta tcccgttgtt cttgtatcgg
     6001 tagagaactc aaccgttgcc actccatttg atgaggtgta agtacagttt gttcgggata
     6061 gggtagcaaa agatgattag caacaagtaa acttaaagca gagccatcaa tcaccagcca
     6121 ttgttctttt gattggtttt ctagtacttg tttgaaacgt tccaaactct tagaagaagt
     6181 acccaatccg ggaccaatca agagtacatc tgcttgcttt attacggaaa gaaactggtc
     6241 agtatccgtc caatctacaa tcattgcttc tggcagatga ctgtgaagag cactccgatt
     6301 tttttcatca gttactacag tcgtgagacc cgctccactg tgtacggcag ctaaagcact
     6361 cataatgatt gctccgccat attgttcatt gccgccaatc aaaacgatac gtccaaatgt
     6421 tcctttatgt gaaatctttg gacgaggtgt gatgactctt ttcactagtt gttcgtttat
     6481 cagcatcgaa aaacctcctt ttactcatta tagcgtatat tttttttaga gaaaaacaga
     6541 taagaaaagg aattgtcaga ttttttgaac tatttttttc tgtaatttgt ggtatcatga
     6601 acacgaagac ttaaataagt ggaggattaa ctttatgaca attgattggc aaaaagaagt
     6661 agaagcacgt aaagaagact tgcttgaaga cttgaaaaat cttttacgtg tcaacagcga
     6721 gcgcgatgat tcaaaagtaa caccagatgc accatttgga cctggacctc gcgacgcatt
     6781 gaaacatatg ttagcttatg gcgaacgtga tggatttaaa gtaaaaaatg tcgataacta
     6841 tgcagggcat attgatcttg gcgaaggaga cgaaacgcta gggatatttg gacacatgga
     6901 tgtcgttcct gcaggagatg gctgggacac agatccttat gaaccagtaa tcaaggatgg
     6961 aaagatcttt gcacgcggat caagcgatga caaaggtcca agtatggcag cttactatgc
     7021 gatgaagatc attaaagaac ttgacttgaa actatcaaaa aaagttcgct tcgtagtcgg
     7081 cagcgatgaa gaaagcggct ggggcgatat ggcttattat ttcgaacatg aagaagaacc
     7141 agattttggt ttctcaccag atgctgaatt cccaatcatc aatggtgaaa aaggaaatgt
     7201 ttctttagct ttacgcttca aaggcgacaa tgcaggagat tatgtcttga aatcttttgt
     7261 ttccggactt cgcgaaaata tggtgccagg aacagcaact gcagcacttc aagtaccaag
     7321 tgcagatgca gctatcgcga tggaagaagc attttaccaa ttcatcgaag caaatccagt
     7381 aagcggaacg attgaagcgg acaatacgta tgtaaaaatc gaattagttg gtaaaggcgc
     7441 acatggtgca agcccacaat ctggtatcaa tgcaggttct ttcttggcat tgttcttaga
     7501 taactatgaa ttcctaggat ctgctaaaca atttatccat gtagcagctg cttatgtcca
     7561 tgaagatttt tatggagaaa aactaggagt cgcttatgaa gatgaaaaaa tgggcaaatt
     7621 gacaatgaat gctggactat ttgcttttga agaaaatggt acagaggaag cgaacttcat
     7681 caatatgaat ttccgttttc caaaaggtgt aacagtagac ggattgcaaa gtgacatcga
     7741 acaaacagtg gggcaagaag gcgcaacagt tactcgaggt gctcgcgtaa tggaaccaca
     7801 ttacgtacca atggatgatc cattggtggc tacattattg caagtctatg aagaccacac
     7861 tggagaaaaa ggctacgaac aaatcatcgg tggcggaact tacggacgtt tgttgaaacg
     7921 tggcgttgct tatggagcga tgttcccagg atatactgat acaatgcatc aagcaaacga
     7981 atttatgagt cttgatgact tgttcagagc aacagcaatc tatgcagatg ccatctatcg
     8041 tttggcgaaa taaaaatact aaaaaa
//
LOCUS       DASHRQ010000093         7731 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.098, whole genome shotgun sequence.
ACCESSION   DASHRQ010000093 DASHRQ010000000
VERSION     DASHRQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.098"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1235)
                     /locus_tag="U2M88_002547"
     CDS             complement(<1..1235)
                     /locus_tag="U2M88_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEM8374875.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQK"
     gene            complement(1228..2820)
                     /locus_tag="U2M88_002548"
     CDS             complement(1228..2820)
                     /locus_tag="U2M88_002548"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEM8374876.1"
                     /translation="MDAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(3330..4055)
                     /locus_tag="U2M88_002549"
     CDS             complement(3330..4055)
                     /locus_tag="U2M88_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374877.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(4209..4535)
                     /locus_tag="U2M88_002550"
     CDS             complement(4209..4535)
                     /locus_tag="U2M88_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374878.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(4572..4937)
                     /locus_tag="U2M88_002551"
     CDS             complement(4572..4937)
                     /locus_tag="U2M88_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEM8374879.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(4974..5189)
                     /locus_tag="U2M88_002552"
     CDS             complement(4974..5189)
                     /locus_tag="U2M88_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8374880.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            5318..5653
                     /locus_tag="U2M88_002553"
     CDS             5318..5653
                     /locus_tag="U2M88_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8374881.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(5729..7726)
                     /locus_tag="U2M88_002554"
     CDS             complement(5729..7726)
                     /locus_tag="U2M88_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374882.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
BASE COUNT         2326 a         1527 c         1091 g         2787 t
ORIGIN      
        1 atcttttgta agaagccttt tttcgtttcc ttcagcaaat ctaacttacg ttgatgaaga
       61 gcgatcgtgt cgtcgagttg tttgaagaaa gcgccgattt tttgttgttc ctctttattc
      121 ctagtaaaag gaaacttgat attagcgaaa ttctcataga ataagtattc tcgtacgctt
      181 ccttcagtgt tgcgcttgac ctctttgata aatgttggtg acttgataaa ctgaagtatg
      241 aactcattat ctaagtcctc gctcattttc acaactacat aaagagagct aacaatgacc
      301 gtcataccaa gattgttaaa tgcaattgat cctacgttta ctcttgctgg attataggcg
      361 aattcatttg gttcaacgaa tttataattt gttttttcta aatcatccaa cctactacca
      421 tcaaactgtt ctgtttgaga aattaaacct gctttattac ttaccgaata ggcagggaag
      481 tctaaatctt tcgtgttctt ttttccagtc ttcttactaa actctttgaa cttacgctgt
      541 tcccaatctt ccgtaaatcc aggaaaccga atttctggca cttttgctcc gtttttaggg
      601 aacatttttt gtaagaatcc ttttttcgtt tcctttaata aatctagctt acgttgttga
      661 agagcgatag tgtcgtccag ttgtttgaag aagttcccaa ttttcttttg ttcttctaat
      721 attggaaatt gtactatcca ttttttcact tctgacattg gtacgagatt tgtaattgct
      781 gaacctggat tagctcctgt gagttttctt tgcataaaat ctgcacgaat aaattgaata
      841 aaatattctg aatctgtgat ttctttattt attctaagtc taatcaaagc ctgattgaat
      901 acacccggtt ttatttgctt tggtactctt gaaattctcc caattgttcc agcgccactc
      961 atgataaaat cgcctggttc taatttaaat ttgattaatt cattatattt tttttcagta
     1021 attttatatc ttgtttcgta attatcataa atagcatttt gttgttcata gacaacaaaa
     1081 ttgctattag atacaaagaa ttctttcttt aaggcactcc caaatggtcc cctccttact
     1141 ccatcttttt tatcaagtaa gcattcaaac ttacgctgtt cccaagcttc cgtaaatcct
     1201 gggaaacgaa tttctggttg tatatcatta ctcatgtttg aacacctcca gcgcaccttg
     1261 taaccacgct tcattttcag gtgttgtttg cagggaagag atagcttcta aaagttcttt
     1321 ttctagcact tgctgttctt gtcgtatctt cttgatgtca tttccgacat gaaccatatc
     1381 aattggttct tcttcttcaa acgtatctac atagcgtgga atatttaagt tatagtcatt
     1441 ttctttgatt tcatcaaagt ttgccaaatg agcgtatttc tcgacatctt ttctttcagc
     1501 ataggtttcg aggatttttt gaatgttttc ttccgataat ttattctgat tttttccttt
     1561 gacaaattct cgactagcgt caatgaataa tacgtctcga gtttggcggt tctttttcaa
     1621 tacaatcacc gtcgttggaa tcgatgtccc gaagaacaag ttcgcaggca taccgatcac
     1681 agcataaata ctgccgtctt ctagtaattt ttgacgaatg accccttctg cagcgccacg
     1741 gaataaaact ccgtgtggca agacgatggc catagtccct gtttccttca aatgatagaa
     1801 accatgaaga agaaacgcaa aatctgcttt ggattttggc gctaattttc cataacgatt
     1861 aaaccgagaa tcgtctaaga aggtcgtatc tgctgaccaa ttagccgaat aaggtgggtt
     1921 cattactact gcatcaaatg tgtaaggttc atctgttggc caatccttat tcaacgtatc
     1981 cccattgcgg agattcattt cttcggcatc taccccatgt aaaatcaagt tcatttttgc
     2041 tagattgtat gtcgttgtat ttaattcttg cccatgatat ttaacgttat ctgggtgagt
     2101 taagtagtta cgcacattta acatcaatga acctgaaccc atggttggat caaaaacact
     2161 aaagaatcgt cgttcttttt gatccaacgt cacgatttgt gccatcatat cagaaaccat
     2221 atgaggcgtg taaaattctc cggctttctt ccctgcttct gaagcaaatt gactgatcag
     2281 aaattcataa gcatccccga tcacgtcccc atcgtgacct aaaacttcca cgtcattcaa
     2341 tttcttaatg acttcggtaa tcgtcacatt tcgttgttgc tcatctgttc ctagtttttt
     2401 tgattggaga tccacatcat caaataaccc attaaattga ttgtaggtag atgccaattg
     2461 cacaaaggct ttgtttagat cattcaattg gaacgttgct tgttttgctt gatccgctaa
     2521 gacgttgaaa agatactctg gcgaaatcgc ataccccaaa atatcaacga ttgttgcaat
     2581 cagatcttct tttgattctt catctgaaag taattcgcga tacaacatcg tttgtttgct
     2641 taccgtatca tattcctcta aggattcatc tgctaaaaga accacttgtt ccaacaaacg
     2701 gtcggataaa tatttataga aaatcagtcc caataaataa tttttatatt ctgacgcatc
     2761 cattttgcta cggagattat ctgccgcact aaataatttt tgatttaatt ctgcatccaa
     2821 aaagatacac tcctatctct tatttaaagt tgctatatac cctcttattt tagcatactc
     2881 ccttttagaa aaagcaaaaa aaaaaagaaa gaaacttatt cttgctaata aactacgcga
     2941 aaggagtcta acgttgtttg atataactta gactgatcgt taacttattt tttatatttc
     3001 tacatagtta tctttagaac catttcagat acatatgaaa caattacata tgattttttc
     3061 ataaaaaaac agtactgtct agtagtataa aatatacaaa tttcttaaaa attttttcac
     3121 taaaatcact acctgaaaca gtctaaaaca cttatatatc aacgtttaat gaaactatct
     3181 ccgtttcatt tcagatacaa ataaatatag tcgggctcca aatccgaaaa aaaatacccc
     3241 cagtagctta tcctaagcta ctggagatgt ataagtacgg tcatataaca acggggtagg
     3301 gtaaataaat gaaaaagatg gaagaatcat tattcaataa cttttttaaa tacttcgttg
     3361 taatcagtta cctgtccgtt cttaaatacc acagaataat cttttttccc attttcaagt
     3421 aaccgaagtg gaatatcttt accaactttc tctgtcactg cattactgat acctgttaga
     3481 ttagctaata aatctgataa agattgagca tctgtagaaa tctctgtatc agtatttatc
     3541 gtgattcctt tatcttcatt tacaagtttc caagtaggtg gtatagctgt gtatgtttct
     3601 acaaatgaat taagtttttc attaagtgac ttctctttta tatttccatc taaaaactct
     3661 gtatatatgg cttgagctat actaaaacaa agctggcctt gttctaataa ttttacatca
     3721 ttagttagta aagctgtgtt cacgttatca atcgcttcaa gaagagtatc tttggtgtcc
     3781 ctaacagctt taggtgtgcc ttctggaatt tccctacttg atacctcgtc cattttatct
     3841 tccataaacc ccttaaaatc atatctttct cccaaatcca tagaagtttt tgagaaagct
     3901 ttcatttctg agtctatttc ttcaataata tttaacatgt ctgatacata ttgtttaggc
     3961 tgattcgttt ttaatctata tgtggtaaat atctcaattc ctccaattat caataaaatt
     4021 gccaaacaaa tccataataa ttttttcttt ttcatttttc ctcctataca ctgctgttat
     4081 aacagtgact aatgtaaaga ttttcccagc aatcatcaga ctattattta cagggaatca
     4141 aaaaaactcc ttagtaagct tacaccttta ggagttcctg tttgaacggt atgcataata
     4201 catttaaatc aatttaacat taacacctca gtatatggta tctctacata tacacggtct
     4261 ttttttggat agtactcgac atttatattg atagcacttc cattgtctaa cgtagcttta
     4321 gcccggtacg tgttatcacc attatcatga gtgatcgtca catcatcaca tgtccgattt
     4381 aagttatact gttcttgtaa aatctgagtt accaatggta cagatgactt ctctagtcca
     4441 gatgaacccg tcattcttga aaaaattata cctataatag ccaatacaac gatacctata
     4501 atgacaaact tcttattcat atttacttta ttcaactttt tatctcctat ctccaaaatg
     4561 attttctctc atcagtaact cattgacatc cccaatagta caccaaagat ggctccagct
     4621 acccctgcta tcatcaaaat cgtacccatt tgttcatctt tctctcgata taagtaaccc
     4681 ataataacag caacagcacc aaataccaca ggaataaatg ctaatgatac taccatagaa
     4741 atccaaccaa taatcaaata cagcttccct gtctcttgtc catgtacgat ttgttccttc
     4801 atttcattaa ttgtctgctc agtacttttc tttactgtgt ctgttccggt tgtctcatct
     4861 gtttcttgta atgctccgca aaactggcaa tacttacttt catttgttat ttctttacca
     4921 cattttctac aatacatgtc ttctcctcct aaaatcattc aattgcttat tagctaactg
     4981 tttactttat taaatacatc agaaaacagc tcatttattg gtacacttaa tatctcagaa
     5041 agcattttca tttgataggg tttaggaatc gttctccctg tttgccacga acttacacat
     5101 tgctgtgtaa cattcatttt tttggctaac tcagtttggg aaagtccttt ttcttttctg
     5161 tatcggctga gattacttac ttttttcatg attctcccct cctgttttat tatatacaac
     5221 aatatgatgt atttcaactc ataaaatata taaatacttc aaatgtcatt acaaatacaa
     5281 caacttgttg tactctactg agaagggggg tgtattaatg aaacagaagc aatatgcaaa
     5341 ggtcattaga gaaaagcgaa aagcactcgg gtggacacaa aaagaattag ctaaaaaaat
     5401 cttctctaca caacaggcag tagcaagatg ggaaaattct gtaacagaac ctaacctcga
     5461 tagcttaaca gctctttcta gagccctagg aacccctgta agccattttc tagacaatgt
     5521 tgacatagat tatgaggaag agtttttggc tctctatcgc tctcttagta ctgaagatgc
     5581 aattcgaaca attgactata tgaagctatt aaaaaggcaa gaaaatgaac gcaatcagct
     5641 attgaaagat tgaaaaataa ataacccacc gaactttaac ggtgggtgta ttgagatgaa
     5701 ataagattaa gagctatgga taacctgtct agtttaatat aattgggtct cttctattcc
     5761 ttactctccg tccatcatct cttgttacac ttgtaaattg ataaatagga actaattcaa
     5821 ctcctgataa tatcaattct tttattactt catctatccc tgcccctttc gctagatata
     5881 ctaaaaaact actgcttgct ttcacattgt atagtttctt aatatcctta gaagctcgtt
     5941 caagagtgaa tccttgttta acaaagtagc ctctgttttc tattaaagca tttttcacgc
     6001 tgttatccct gacatttcgt aggcgttcac aaacagtgtt aataagttct ttttctgtca
     6061 ttagatatgt cccttgccac gataagccct gtaaaacttc atgtgcttca tctaaaatca
     6121 attgtttttc cttgctaagt tctgatcggg aattttgagg aaatactctt ttagcctctt
     6181 tttcgttaaa acatatctta acccattcat aagaaaaata ttgatatgta aaatgaaatt
     6241 tttccagaag tggaaggata tcttcattca atttttgaat cacagaagtt gcattaaaaa
     6301 cgtctaatcg atagacattg cttctcttat gtttaaagtt tcctaacgct ttctcttcct
     6361 ttaacctatt taggtattcg agcatcttca aaggaacatc ttcatcatct aattttttta
     6421 tcaatcctat aaaagtcata agtgatagta acttattaca tttatctatg tcataagttt
     6481 taccatagta agaactcatt tttttagcta attgttcccc cgacaaaata caagtcacac
     6541 tttccttact atcatcaaaa aaattaacat cagaaagcaa gacatcatag acacgtcgca
     6601 tttcctctag ataattcctt attgtcctga aaagtttgtc tttcattgat tcaagagtgt
     6661 caaaaagtac ttttgattcc tctttcaatt ttcgaaaatg tgtaggatac aagtctaggt
     6721 tctctaatag ccaattaaat gtttctaccc gactgtcttt ctttaaaatt agcatcacaa
     6781 ctccaataaa attttcactg atatcacaat tactggaatg acaataaaaa atggaatgcc
     6841 cagttttact ggtaaaaata ctagcagagg gattatgatc gttgtgaaac agacatctta
     6901 agtttttact tgacttaagt aatttattca tatcaatggt taacaatctt tcataaatct
     6961 cattaaaaga catgtcggag gaatcaaaca ggcagtcttt aaaccactgt ttcatttctt
     7021 ctttgtcgtc attttttatc aacttaacaa aatcggtttc attgactagt tcatactctc
     7081 tccctgaatc actttgacaa tttttagaac atgtcatggt aaaatcaatc ggaagctcag
     7141 taatatctaa catgttgtct tcattaatta ttatcactgc atctgactta tatcctccaa
     7201 aaaacattct attcgaagca tttacattgt catcacaccc ggggatgttc tttttcaagt
     7261 attcgtaaac agcaaccatt ttaccatagt cagtaattgc ttcttttaat agaaagacaa
     7321 tcttgtattt ttctagattt tcagagtacc gaatactttt ttgtatcgca aacgcagaat
     7381 tatgaactaa attgatagat aaaacctttt ctatgcttaa gtatctacta tcatctttac
     7441 tcaaatctac taccttcccc ttctcatctt taacagtgtt atcgacatcc agaataatta
     7501 ctctggtttc cacaaaactc tccttgtcat tgcctttatt ctttgtcttg cttggaataa
     7561 atgattgtga tgaatttagt atctctacaa actcatctct tgtaacttct ctatagttat
     7621 ctaaacacct atctcttaca tcagtttttt ctttttttgt aactggcttc tctaataatt
     7681 tttctttatc tacataaatt ggaatattaa tcattgatgt ttccatttgt g
//
LOCUS       DASHRQ010000094         7606 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.099, whole genome shotgun sequence.
ACCESSION   DASHRQ010000094 DASHRQ010000000
VERSION     DASHRQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.099"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(37..642)
                     /locus_tag="U2M88_002555"
     CDS             complement(37..642)
                     /locus_tag="U2M88_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEM8374883.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(838..1302)
                     /locus_tag="U2M88_002556"
     CDS             complement(838..1302)
                     /locus_tag="U2M88_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEM8374884.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(1324..1644)
                     /locus_tag="U2M88_002557"
     CDS             complement(1324..1644)
                     /locus_tag="U2M88_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEM8374885.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(1787..2866)
                     /gene="pepA"
                     /locus_tag="U2M88_002558"
     CDS             complement(1787..2866)
                     /gene="pepA"
                     /locus_tag="U2M88_002558"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEM8374886.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            3066..3416
                     /locus_tag="U2M88_002559"
     CDS             3066..3416
                     /locus_tag="U2M88_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEM8374887.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            3601..4554
                     /locus_tag="U2M88_002560"
     CDS             3601..4554
                     /locus_tag="U2M88_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEM8374888.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            4567..6354
                     /locus_tag="U2M88_002561"
     CDS             4567..6354
                     /locus_tag="U2M88_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374889.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            6341..7543
                     /locus_tag="U2M88_002562"
     CDS             6341..7543
                     /locus_tag="U2M88_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374890.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         2168 a         1446 c         1366 g         2626 t
ORIGIN      
        1 aaaaagaggt tgtgacaaag acttgtcaca acctctttat tttggaaata cttctccgac
       61 tacggcatcc tcaggcaata ctaagattcc ttttttttct ggagcatttg gcaaatgcaa
      121 ttcacgagca gaacaaatca ttccatagct ggctacccct cgcaaagttc ctggccagat
      181 catcattcca tctggcatca tggctcctgg ttttgcaacg acaacttttt gtccagcttc
      241 gatatttggt gcaccgcaaa caatttgcaa tactgtccca ttgtccactt ctgtctcagt
      301 aatcgataaa tggtcagagt caggatgcgg gacgcatgtt tttacatacc cgacaacgat
      361 cttcggttca tgatcagctt caagctgttc ttcgaatcct gcttctttaa tcaatcgatt
      421 caatttcgct aattcttcat ctgttaagct tacttggccg ttcccttcaa ttgttagatg
      481 atcggagata tggaaaaagt tccaagcaac aacttgacct tgttcatctt tgatccgagc
      541 aacattccat ttacgttcga cagtgttttc tgttccttta tcgtcggcta tgataaccat
      601 caaagtgtct ccgacgtgtg ctttgttata agcaaaaatc atgttctttc ctccattatc
      661 ctttgctgag atttatctta acaaaaaatt gcattcactg catgagattt ttttcatgct
      721 ttatttccga agaagcttct tctggaacac catttattcg taacaaggga ctgtgacaag
      781 acttttgtca cagtcccttt actgtttgat taaaaaggtt atacaagttc cacttgctta
      841 attactcggc tgttcttctt cgtcagaaac gatcaagaca tcacaaggtg aatgacgtat
      901 gacgtagctg gctacacttc ctgtcataaa gcgttcgact gcatttagtc cagactgccc
      961 aaccatgatt agatcgattg catatttttt tggcaatttg actgccatgg attcacgaac
     1021 agagccgtat gcaataattc cttctatatt tgtgtaatca acagattttc catattcttt
     1081 acattcttcg atcatttgct tggctgtttc agtttgctga tcgactagat tttgatcaat
     1141 aggagcatag cccataaaat tataaaattg ctgatcgatt acgtttgcta catatacttt
     1201 cccattatta cggcgagcca cttctaacgc ttttttgaaa gcagcaagtg cttgttcact
     1261 accgtcaata cctactaaaa tgttcttata ttcttgattc atcttgaccc ctcctaacag
     1321 atgtcagcct tttattacac tttctaggaa cgattcgatt tcctgttttg ttttacgatc
     1381 tttattcact agacgaccta attcttttcc gtcttggatc acgacaaaac ttgggatacc
     1441 aaaaatgttc cattcagcgg ctacatctat gtacttgtca cggtctactt caataaattg
     1501 ccaatcagaa aagtcatttt cgatttcagg catttgcggt ttgataaaac ggcagtctcc
     1561 gcaccagccg gcagtaaaga aaaatacact ttttccagtt tctacgtagc ctgctaattc
     1621 ttctaaacta tttgggataa tcattctttt tcctctactt tctgtttatg tatttttcaa
     1681 ttaaattata cacctttctt tcgaactaac aaacattgat cctcttttta taaaaaaaat
     1741 ctgacaaaag gttgcttttg ccagatttta tagtttcact atcgcgttat ttcccgtata
     1801 cgatcgtgtt cactgttgat ttatctaaac ctttcaatgt ttgaatcaac atttctcgtg
     1861 cagcttcaaa atcgcgaata ctgaacatcg tttgatgcgt atggatataa cgtcccacta
     1921 cgccaatcac tgtacttggg attccttcat tttgtgtatg agcagcacct gcatctgtac
     1981 cgccttttga aacaaagtat tgataaggga tgttgtttgt ttcagcgata tctagcaagt
     2041 actcacgcaa acgaggaagc atgatcaaac ctggatcttg gatacgcatc aatgtacctt
     2101 ctcctaaatg tccgaatgtg ccatttttcg tcactgtgtc atctgctgca gagcagtcta
     2161 ctgcaaagaa cagatctggc ttgaatttat tgacagaagc tttagaacca cgcaagccta
     2221 cttcttcttg gacattcgcg cctgcaatca atgtatgacc taatgtttca ttttgtaacg
     2281 cttctaaagc ttctaatacc attgtacagc catagcggtt gtcccacgat ttgctgatga
     2341 tattcttccc gttggctgtt ttgatcgttt cagcgtaagg aacgattgtg tctcctggaa
     2401 gaacaccgta actcattgcc tcttctcttg attcaaaccc agcatcgaac aaaatatctg
     2461 ttacttctac tgatttttgt ccgcttgttc ctcgtaataa atgtggggga acggaagaag
     2521 aaatacatgg ataattctta ccagtagagg ttttaagtgt aaaacgttga gcagaaacaa
     2581 cgtaagggtt ccatccgcct agaggtacga ctttaaaaag cccgttttct tggatttgtg
     2641 tcagcatgaa tccgacttca tccatatgtg ctgcgaccat tacacgaggt gcatccgctt
     2701 cttcatggtg gcgcagccca aaaatgccgc ccaaaccatc taactgtaac tcatctacca
     2761 gcggttccat atgactttcc atataggcgc gaacgtcttg ttcaaaaccg cttgttcctt
     2821 gtaattctgt taactctttg attcgttgaa atgttttttc ttccatatgc tattcgtcta
     2881 aatttcagac taaactcctc tcagacttat attgatctgc ggataaaata tgattttttc
     2941 tgcagaccga tcgtatttat tatagcttat tttttctggt agcggttatt tatttcaaag
     3001 aaaatatgag agtgtggtaa aataaacaca aagagtagca agtgagaaga aggaggtata
     3061 tccacatgta tgaagaaagc gaattaagct attttaaagg cgggttagcc gttgggatcg
     3121 gtctaggatt attgagtggg gtagcttcaa cgatttggta tcatcgcaaa aaagtgttat
     3181 cagcagacca agtgcttgaa aatatcaaaa catctttttt aaaagaaggc gcaatcgaag
     3241 gtgcttggat ctcttttgaa aaagaaccta ttcggaaatt cgccgttcgg tcgagtgctt
     3301 atcggggtgg gctttcccgg atagaagatg gcgaaactgt ttattatgaa tttttagcag
     3361 atgctcatac aggaaccgta ttagacattt caagaaaaaa gggcgcaata gattaatacg
     3421 tcaaaaaaat gaaataacga atacgcaccc actatctaaa agagaggtat cctatccttc
     3481 ttttatagtg ggtgattttt ataaaaatag ggctatttcc tcttggaata gctagtattt
     3541 gtagttatta tacggtattc ttgaaataag attagtttta ttatataagg agagtgacaa
     3601 atggaatcac gtgaaaaaat tgccgtgctc attccttgtt ataacgaaga ggcgacaatt
     3661 tcaactgtta ttgaagattt taaaagggaa ttaccagaag cagatattta tgtctatgat
     3721 aataattcga ccgatcgaac atacgaattg gccgttcaag gcggagcaat cgtaaagaaa
     3781 gaaccacgtc aaggaaaagg gaatgtaatc cgtcaaatgt tttttgatat tgatgccgat
     3841 tattatttga tggtagatgg tgatgatact tatcctgcag aagctgcaca tggtttgctt
     3901 gagaaattac gttctggcga agcagatatg gtcattggcg atcgtctatc aaatggaacc
     3961 tattttgatg aaaataagcg tcctttccat gattttggga ataatctggt taaaaataca
     4021 attacttact tatataaaac aaaaattcgc gatgtcatga ccggctatcg aggattcaat
     4081 cgaatttttg tcaaaagctt ccctattatg agttctggtt ttcaaatcga aacagaattg
     4141 acgattcatg ctttagataa aaaattcaag ttcgttgaga ttccaatcga ttatcgcgat
     4201 cgtcctgaag gtagcgaatc aaaattgaat acattctctg acggattcaa agtgatcatg
     4261 atgatcatca aaatgtgcaa agattataaa cctttgcttt tctttgggat ctggaccatc
     4321 ttcttctttt tatttggatt ggtcatgggg attcctgtga ttcgtgaata tatgctgaca
     4381 agctttatta cacgcatccc ttctgcgatt ttaagtactg gattgatgac tttagccctt
     4441 ctttcattag taacagggtt gattttggat acggtcgtca caaatgccaa gaaagaatat
     4501 gaattgaatt tataccatgt ctaccacgaa tatcaaaata aagaaatgaa gtaggaggcc
     4561 ttttttatgc ataagaaaaa ggagaatcta tttcctactt tttttgcaac tttatttgcg
     4621 tcaggttttg ctttttttac ttcttatggt ctaggaactt tgaatccaac agtaatcatt
     4681 ggtttagcca tggttttgct acttgctttt aagcaatatg gaatagagtt gttttcaata
     4741 aaaaacaaag ttgcagctat tgcccgattg tttttttctg cagcttttgc tcttacactt
     4801 tctattggca gacagataca atttcagtat gtctcagcaa actatacaaa gacaacgatt
     4861 gctgatggaa gtatttttta ctttcttgtt tatttctcca tttttgtgta ttctttttta
     4921 tttttacaat cgatttattt atcaatgcca aaactggaaa cattttttac tgctcaaaac
     4981 acacaggctt tctcttcaag aaaatatttt ttattcttta ctgtcttttt agttctgtgt
     5041 tggcttcctt atctgttggc attgtatcct ggagtggttt tgcctgattc gttaagttcg
     5101 gtcgcccaat ctatgggtga tgttcctata agcaatcatc atccagtctt attcacctta
     5161 ctggtcaagc tatttgttca tgatttacca actgatacga tcaatcacgg tgtattttta
     5221 ttttcgattt ttcaaacatt gataacagct tcagcgataa gttattctct ttgctggctg
     5281 ttaaaaagaa acatccattt ttttccggta ctcattagtt tatgctattt tgcatttgca
     5341 ccggtttttc caatctacgc cttgaacatg caaaaagata ctttgttctc tatcgcttgc
     5401 tttttatttt ccttagcggt ttttgacttt atttttcaac gagaaaagcc aacgattctc
     5461 aatcagattt acgtaatcat ccttgcttta ctgaccacct atctaagaaa taatgggatt
     5521 tatgtagtaa ccggcacagg tttgatttgc ggcttgatta ttttgaaaca aaaaaggtat
     5581 caagcattct ttcaagtggt gctgggttat acagtgattg ctggactact catccacccg
     5641 ttgatggctc gttatgctat tcctacagaa actgctgaac aattaggtgt tcctttgcag
     5701 caaatttcta gaactattgt tatggatggt gacttgtcta agaaagataa agcattcatg
     5761 aatcagctac tcccgataaa agattatgaa gcgtattctc ctagtcttgc agactcaatc
     5821 aagtggcata aaaattttaa caatgagttt ttgaatacac ataaaaaaga atttctatca
     5881 ctatgggcaa gaaatttacc aagtaatata aaaatctata cagatgctta tattttagaa
     5941 acgttcgggt tctgggtacc tggtacaaaa aatagttacg gctttttaga tactcgtgta
     6001 aacacgaata attatggtat gaagcaagta gatttgattg aacatcttac tggattgtca
     6061 ggaatgaaaa aaatcatcga tgctcgtgat ttctttggtt ccggcacatt actgtggtta
     6121 gctatcggca gtgcactttt agcaatcttt aagaaaaaat atcggtattt actccttttt
     6181 cttcccggta tgcttgtttt tgctactatt atgattgcta ctcctgtggc ttttagttta
     6241 cggtatgtgt ttgttttagc attaggtcta cctgtttata tcctctctcc ttttttacta
     6301 gataaaagag aaaaaatgaa aacagatata ggagatactc atgaaactat ttaaaaagat
     6361 attttttgct tttgttctct tgatcgctag ctattgcgta ctactgacaa ttactttttt
     6421 cattccgcag cactctattg aatcgaatgc agaaaaatct ctacagatgg ttgaaaaaga
     6481 aggaatgtat ccttctatca atcaaggaaa tatgctaggc actcggttag ataattttac
     6541 tgatcatttg atgatacgaa aaacaaaagc agatcctgaa ttgaactcct tggagaacgc
     6601 gatgtctatg gcggattatc cccgttattg gcatggttat cagctttttt tacgaccttt
     6661 actgctaggg atgagtttgg ggagtattcg aatgatttac gcagccattc tttttctgct
     6721 gattggattg acgagctact ttttaaccaa gtgtagcgac atctatttaa cgacagcctt
     6781 gttgatttcg cttgctatag gaaatgctgc aacctttttc ttcagtatgc aattttcaaa
     6841 tatttggatt ttgactttat tgtctgtatt actattttta acaaaaccac aactctccaa
     6901 caccaaccaa caattattct tgtatttctt tgtcattggt tctttagcta acttttttga
     6961 cttgttgaca acaccggtca tctcttgggg gatacctgtg atcattattt attatgtaac
     7021 aaataagtat ttaatggata aacgcagttc attgagcaaa caagtaggca gttttgtttt
     7081 taccggtatc ttttggggat taggctatgg tttgacatgg gtggctaaat ggatcctatc
     7141 atcgatcata ttgaacaaaa acattgtaaa agatgcaatc aaccaaatcc tctttcgaac
     7201 ggaaggaaac gacaaatacc cgttgcatcg attagagatg ctgaaaagca atattagatt
     7261 gatgtatcca aaagtagcaa tattattgct attgattact tgtcttgttt ttctctattt
     7321 tgcttatcgt aaacgaaata gctggcgtcc gttccaactt ctagttttgt ttgttttagc
     7381 tgctgtaacg ccgtatattt ggtacaatat tttagctaat cactcacaga ttcattattg
     7441 gtttacgtat cgtacgcaaa tcatcacaag ttttgctata cttagtgctt tcgccttttt
     7501 gatccctcct gagaaatcaa aagacgaact aaattacttt taagaatacc gatgtactaa
     7561 ttcattttta aacataaaaa ctccagctgt cagagatagc cggagt
//
LOCUS       DASHRQ010000095         7594 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.100, whole genome shotgun sequence.
ACCESSION   DASHRQ010000095 DASHRQ010000000
VERSION     DASHRQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.100"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(939..1352)
                     /locus_tag="U2M88_002563"
     CDS             complement(939..1352)
                     /locus_tag="U2M88_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEM8374891.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1808..2293)
                     /locus_tag="U2M88_002564"
     CDS             complement(1808..2293)
                     /locus_tag="U2M88_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374892.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2432..2647)
                     /locus_tag="U2M88_002565"
     CDS             complement(2432..2647)
                     /locus_tag="U2M88_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEM8374893.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2871..3671
                     /locus_tag="U2M88_002566"
     CDS             2871..3671
                     /locus_tag="U2M88_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM8374894.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3690..5558
                     /locus_tag="U2M88_002567"
     CDS             3690..5558
                     /locus_tag="U2M88_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEM8374895.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5551..6042
                     /locus_tag="U2M88_002568"
     CDS             5551..6042
                     /locus_tag="U2M88_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEM8374896.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6030..6584
                     /gene="srlA"
                     /locus_tag="U2M88_002569"
     CDS             6030..6584
                     /gene="srlA"
                     /locus_tag="U2M88_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEM8374897.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6602..>7594
                     /locus_tag="U2M88_002570"
     CDS             6602..>7594
                     /locus_tag="U2M88_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEM8374898.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1441 g         2233 t
ORIGIN      
        1 ttactatgaa taacataata agaaagtaaa gtggattgat ttgagatctt tttatacttc
       61 aaaaaagata acacccaact ttcaaaaata tgttttggaa gttgggacaa acgagttaat
      121 acattttaat acattatttt ttattcaaaa aaaatagcac aatggcaaag atcgtaagag
      181 agatcataag ccccaatgaa tgactaaaaa gaaaagcaga atcacatgcc atatcatccc
      241 atatttcttc aagaaaataa ataagcggga aaaagggaag gaatagtaga acatcgatcc
      301 agcaccattt ttttcttctt ttgaaataac ataaaacaat aaacatgaac gtgcagggaa
      361 gtaaaaacaa agctttactc ataactaaca aaataccagc tccttttatt tttttctccc
      421 ccacattcaa agagtaacaa tggtatgtac ttaataatag aaaaatggat caacttgtat
      481 gaataacagc tgaaaagatc tgcaccaaat aaaaaacttc cagccaaaac tgactggaag
      541 tttatttttt tctgaaatat tctaacacaa atatgaaaaa gatggccgaa gctaataagc
      601 cgatttcgta gcaaaacaat gagataccag agtcgtaata ataaaatcct ctaatcttat
      661 ggataaaata gatcatcgga caagcagata agagtaagta tttgttaggc cttttgaata
      721 agtaaaagaa agcactggtc attatgataa atggtatcca aaacaaaagt ttacttaaga
      781 caatcaaatg atcaactccc atatttttta ccaagagaga tcataacatc tttatcgtaa
      841 aaacaaaata tttttttgct cattttgaaa atttaaacat actcgttctt atccaaatat
      901 ataaaaatcg cccctcattc atcgagaggc gataagttct aatttaactc aatttttttt
      961 gtttcttttg gctgataagc tgttttaggt aataccaatt tcagcacacc gtttttaaag
     1021 gttccagtga ttttttcttc atcaacatta ggaagataga atgaacgttt gtaagagacc
     1081 gaatgtcttt ctttccgtac atagttgcca tcttctttat cttctgtatg ggactcatga
     1141 ttggcggaga tcgtcaaagt attatctgaa tattcaactg tcgtgtcttc tttgtcacag
     1201 ccaggcatat cagcagttag tttgtattct ttttcatttt cgactaaatc tacttttgga
     1261 taacttgaaa cacctaaaaa atcattgaat gctggactaa acacgtcatt gaaatcaggg
     1321 aacatgtcgc gaatgctagg tacatttgcc atttttgatc atcctttcaa taagtatttt
     1381 ttatttatcc ctatttacgt aataactcta ttttgaaaaa aatgcaagta taatggttag
     1441 ctttgtgaat atttcatcta atacgataca aaggctatct ttctaatttg aaaaagggat
     1501 cattaaaata ataagaagaa aatatgtcaa aaggtaaaaa agtaaattct tctatccaaa
     1561 tatagaaaaa ttgaatctag aaaaccgccc gacaataaat ttcattaact cagttgaccg
     1621 aaaatgaagt agataccaaa aatttatcaa gagagcaagt agagaagtga gtatattcat
     1681 aagagaaata gctgtaacga attattaggt taatgcaaat gcttatttat actccgtata
     1741 actttctcag aaaaaatgat accaataatg acttgctagt actgtaaatc cgtataatat
     1801 catctaatca aaatggtgtt ccatttgcat ctgtcatcgt aatcgatcct tcgggtgaca
     1861 ctcgataaaa accaacgctt ccagatcctc cttgggaacg gatttgtttg ctggaagctg
     1921 tgaacaaata atctcgatca attttttgat aaaagctaat aacaatatcg tcactttgta
     1981 attcggaacg gtgggcaata agaaatagaa gtgctttgtc agagttttct aatggaagag
     2041 aaggtacagt gctttcttgc atttcagatg ttgaagaaat gctaaacgac tgaatacttg
     2101 attggctatg ttgcgaatct acattaccag tagaagtatt agtaatggaa gtagtagaag
     2161 tacttttagt tgaaccaacc tcagttgttg aaatggctgc tgaagattct ttaacagaat
     2221 tctttgtttt atatccacag gcagaaaaaa gtattacagt tcccaacaag cctgcgtaaa
     2281 tccctttatg catgatagct cctcctaaaa tagattatct ttattcagta tatcacttaa
     2341 acaaagaaaa gcagatggtt taaaatgaaa aaaccttccc aataaaggga aggttcatga
     2401 agataataac ttttttaata attatagctt tctaatatct ttcatcaact tttctagtaa
     2461 tcattgggaa aaatttcaag cagtagatcg cacaaatacc tacgacgata taaatgatct
     2521 tcgcaaaaat agttgttgaa ccaccagcaa tcatagctac taaatcaagt tcgaacaagc
     2581 ctaccaacag ccagttcaac cctcccacaa tcaataaagt taatgcaata gcatctaaag
     2641 ttttcattag aattctcctt tcttaaatga taaaacaatt atatatcaaa aagagaaaca
     2701 ttgcttatat aacgctcagg aattttagca atctatgcaa aaagatgaaa attttttttg
     2761 ctcttccatt agtacacatt ttttagctga agctgtaaat ttcatgtctt aatttttttt
     2821 agtgagcttt tatactggat ttgtaagaaa aaagaaggag aggatcacat atgtcggatt
     2881 ggttggatat tgcaggaaaa gtagtaattg taactggagg ttcctcagga attggaagaa
     2941 gtatcgtcga aaatttgttg aagcagaatg cacaagtagc gaattttgat gtaactgaat
     3001 gccgtataca gcatgaaaac ctactatctt taaaagtcga tgtgtcttct aaaacggaca
     3061 tagaagaagg gatatataaa gtaatgaaac atttcaagac gatcgatggt ctagtcaata
     3121 atgcaggaat aaatattcct tctcttctaa tagatagaaa tcatccaaaa agcaagtatg
     3181 aattatcaga acaagttttt gataagatga tcgctgtcaa tcaaaaaagt gtatatttga
     3241 tgagccaagc agttggaaga atcttggtgc aaaagggatc aggagtgatc gtgaatcttt
     3301 cttctgaatc aggattggaa ggatcagaag gacagagctg ttatgctgct acaaaagcag
     3361 cgatgaacag ttttactcgt tcttgggcaa aagagttagg aaagaggaat gttcgtgtgg
     3421 taggtgttgc accagggata ctggaagaaa ccggtttgag aacacaagaa tatgaagaag
     3481 ctctttctta tacgagaggt ataagcgtcg aacagctgag aaacggttac agtcgaacaa
     3541 gtacgatccc tctaggaagg agtggtaagc tgcaagaagt ggcggatcta gtctgctatt
     3601 atctttcaga aagatcaagt tatatcactg gagtcacgac taatatttca ggaggtaaaa
     3661 caagaggata aaggataggg gggacgcacg tgacgctagt aaacagatgg taccaattat
     3721 tgaaattact agtcactcac aagaaagttt caatccagga agtgcaaaaa gaaatcaaaa
     3781 ccagtaatca aaccataaaa aaagatattg gggagttgaa tgaagaaatt gccggtattg
     3841 caaaaatcat ccaaaaaaac aaacattttg aacttgaaat ttatgatttt gatttttttg
     3901 atgagatcat gcaaggaaaa ttgaaaacag ccagtgattt caattcggca tccaaaagaa
     3961 tcgcttattt actaaatagg ctgatcgaaa cagaggattt cttacgtatg gatgatttgt
     4021 ctgaagaagt cggtgtcagc agaggaacag tggtcaagga tctaaagaca ttaaaagaga
     4081 tgatcaatga ttttgatgta aagattacag gcacaccaaa taaaggacta agaataatag
     4141 gagatgagct agaattacga ctacttttat tttattttgt ttctccctac ttttcagaga
     4201 cgaaaacaga acagtttcta gaagaaaaac aagcaatcaa acattttcag aagaagacga
     4261 atgctagtaa acaagaaatc acccttttaa cgaaagttat cggtatttct ttgaatagaa
     4321 tctctagtaa ctatctgttg aagaagccca tcaatcgtta cagtgggtgt tttgaatata
     4381 tccaagaatt caatgaactg atcaatcagt tggaagaaat ctatgaacta actttgagcc
     4441 aatatgaaaa agattttatc agttttcctt tgaacatcat caacacaagt ataacgatga
     4501 taaggaaagc taactctgat ttgcgcttat atttcgaccc catgatgatg cgtattcgac
     4561 gattagatct cattgatctg gatgagtcct tcttatatca agagatgaaa cagcacctct
     4621 ctttattgat aaatcgggta atttttcgtt atcgattgac tgatttattt tatggtgaga
     4681 tagaaaaaaa gtatcctttt tcttaccaaa tcgctagcga atgcatacaa gcattagaag
     4741 ctatcttgca acataagatc tctaaagtag agatcagcta cctaacgttg tatttggagt
     4801 tagcatttcg atatcaaaaa gagcattttt cctctaaaaa agtagccatt gttcatacga
     4861 caggaaaagg aacagcatta atgatccaaa gacaaatcaa aaggatattg gggagggata
     4921 tacaaataac acatgtttca gaagaacaat atgaaaaagt agatctaaac aaatatttcg
     4981 ctgtttttac aacgattccg ttaaaaaatg taaatgaaga gacaccggtg atccatttga
     5041 ctaatctttt tgatgactct tggttaaggg cacaatggca aaaagcaaaa aaaaagcgat
     5101 tagccgggtt tcattctact gtttttttct atcaaatatg gaaagaaaaa aagacgtatc
     5161 aagaatattt aaacaaatta gtcgctcctt tgatcagagg ctattatgtg gatgaagaat
     5221 ttgaaaaaag gatcttgaaa agagaatatc agcaaagcac atttgttaac aaagaatttg
     5281 cttttcctca cacaaccaac cagctatctc aacacattgt tttatcagta gggctaataa
     5341 ctgatcaagg tttagcagaa acaggaggaa atggagttat ttttttattt gccatacctg
     5401 aagaaatgac ggctgaagtt gagaatgaat tatttgagct atacgatatc ctatttgaac
     5461 tcataaaaat gggaatagat gagtttattg aattagcacc gacacaagaa tcatttttaa
     5521 aactaatcaa aagtaaagga gagagacttc atgaatgata tgtatctatt catagttttc
     5581 gcagcttcag cttatgtgct gcaagcagtt atcagttgga agcaactaaa gcatttcaat
     5641 caaacatata gcgaactgcg aagaaaagga aaagtagcga ttggtaaacg acctgggaaa
     5701 atccgttcag gcacgattgt tatgctagct atagacgatc atgatcaagt tttagaagtc
     5761 agaaaaatgc aaggggttac atctttggca aaatttcatt cattacctgt ctatgcgggt
     5821 gaagatatcc attacatcga tcgttaccat ccctcagttc gagcggaaga tcgtttgaca
     5881 caagcagctt tagaaaatgc cagagaagta tacttacgaa tagaggcagg aaatgaaaaa
     5941 agtgaacaac cttcattgcc ttttattgct atgctcaata attttcggat atggaaaagt
     6001 acgatcaaag aaaaaaggag tgacaaacga tggatatcat aacaaaattt gctgagggat
     6061 ttatgaggct gttccaaaca ggtgcagaaa cattcataag ctggatgaca ggaatcgtac
     6121 cagttgtctt gatgctgatg gttgcaatga atacgctgat tgctttttta ggtgaagacc
     6181 gagtaacgaa agtagccaaa ttatcagcga aaaatccgct tactcgttac cttgtgttac
     6241 cgttcttatc tgcttttatg ttgggaaatc ctatgtcatt tacaatggct cgtttccttc
     6301 cagaatacta caaaccaagc tattatgctg ctcaagctca attttgtcat acgagcaatg
     6361 gtgtattccc ccatattaat cctggagaat tgtttgtatg gatggggatt gctcaaggga
     6421 tcgaaacctt agggctaaat tcgatggagc tggctatccg atacttgctt gtgggactgc
     6481 tgatgaactt tattggtggt tggataactg attttactac tggatttgta tgcagacagc
     6541 aaggaatcgt tcttagcaaa aaagtagagc tttcagttga ttagagaaag gagagtttta
     6601 aatgtatggt gatattaaga tcgtaagagg aaacggcggg tatggaggtc ctttgattat
     6661 taggccgaca aaagataaac acaagttcat ctatgtcaca ggtggaggtg agaggccaat
     6721 gatcgtagat aaaatagaag aactgacagg tatggagtct atcaatgggt tcaaaacatc
     6781 aatacctgat agtgaaatcg cgcttgcaat cgttgactgt ggagggactc ttagatgtgg
     6841 aatttatcct aaaaaaggga taccaaccat caatatcgtt tccacaggaa aaagtggccc
     6901 attagcacaa tatatcactg aagatattta tgtttcagca gtagggttag aacaaataca
     6961 gccactaatg gaaactgatg ataacaaaga aacagtaaat actgcatctc cagataaaaa
     7021 gtacaaatat gatacttcta aaaagataac cgagcaaaaa gcagagcagc aaaactggat
     7081 agcaaaaatt ggaatgggcg cgggaaaagt agttgctaca ttcaatcaat cagcaagaga
     7141 agcagtagat acgatgatcc ggacagtgat cccttttatg gctttcgttt cccttttgat
     7201 tggtgtgata caaggttctg ggatcggtaa tgtatttgct aacattatgg cacctttagc
     7261 aggaaatgca gttggattaa caatcattgg ctttatttgt tcactgccat ttttatctcc
     7321 tttgcttggt ccaggggcag ttattagcca agtgattgga acattgatcg gtgtagagat
     7381 tgggaaagga aatattcctc cccaattagc attaccggca ctatttgcta tcaatactca
     7441 aaatgcatgt gattttattc cagtcggtct aggtttggaa gaagcaaaag ctgagacagt
     7501 agaagtaggt gtaccatcag ttctttattc aagatttctt aacggcatcc cacgagttat
     7561 cattgcttgg gtagcaagtt ttggattata tagc
//
LOCUS       DASHRQ010000096         7215 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.101, whole genome shotgun sequence.
ACCESSION   DASHRQ010000096 DASHRQ010000000
VERSION     DASHRQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.101"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..445)
                     /locus_tag="U2M88_002571"
     CDS             complement(<1..445)
                     /locus_tag="U2M88_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEM8374899.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGA"
     gene            complement(557..937)
                     /locus_tag="U2M88_002572"
     CDS             complement(557..937)
                     /locus_tag="U2M88_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEM8374900.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(934..1320)
                     /locus_tag="U2M88_002573"
     CDS             complement(934..1320)
                     /locus_tag="U2M88_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374901.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(1322..1600)
                     /locus_tag="U2M88_002574"
     CDS             complement(1322..1600)
                     /locus_tag="U2M88_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374902.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            1648..2376
                     /locus_tag="U2M88_002575"
     CDS             1648..2376
                     /locus_tag="U2M88_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HEM8374903.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(2522..2998)
                     /locus_tag="U2M88_002576"
     CDS             complement(2522..2998)
                     /locus_tag="U2M88_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEM8374904.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(2958..3305)
                     /locus_tag="U2M88_002577"
     CDS             complement(2958..3305)
                     /locus_tag="U2M88_002577"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374905.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(3292..3477)
                     /locus_tag="U2M88_002578"
     CDS             complement(3292..3477)
                     /locus_tag="U2M88_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374906.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(3478..3750)
                     /locus_tag="U2M88_002579"
     CDS             complement(3478..3750)
                     /locus_tag="U2M88_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HEM8374907.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(3747..3950)
                     /locus_tag="U2M88_002580"
     CDS             complement(3747..3950)
                     /locus_tag="U2M88_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374908.1"
                     /translation="MVKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            4075..4287
                     /locus_tag="U2M88_002581"
     CDS             4075..4287
                     /locus_tag="U2M88_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374909.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(4320..4514)
                     /locus_tag="U2M88_002582"
     CDS             complement(4320..4514)
                     /locus_tag="U2M88_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374910.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(4522..4710)
                     /locus_tag="U2M88_002583"
     CDS             complement(4522..4710)
                     /locus_tag="U2M88_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374911.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            complement(4707..4847)
                     /locus_tag="U2M88_002584"
     CDS             complement(4707..4847)
                     /locus_tag="U2M88_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374912.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(5337..5702)
                     /locus_tag="U2M88_002585"
     CDS             complement(5337..5702)
                     /locus_tag="U2M88_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374913.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(5742..6044)
                     /locus_tag="U2M88_002586"
     CDS             complement(5742..6044)
                     /locus_tag="U2M88_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEM8374914.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(6044..6343)
                     /locus_tag="U2M88_002587"
     CDS             complement(6044..6343)
                     /locus_tag="U2M88_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374915.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(6336..6500)
                     /locus_tag="U2M88_002588"
     CDS             complement(6336..6500)
                     /locus_tag="U2M88_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374916.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(6506..6775)
                     /locus_tag="U2M88_002589"
     CDS             complement(6506..6775)
                     /locus_tag="U2M88_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374917.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(6787..>7215)
                     /locus_tag="U2M88_002590"
     CDS             complement(6787..>7215)
                     /locus_tag="U2M88_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8374918.1"
                     /translation="EKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKN
                     VLLSDEQMEKLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNA
                     SSGYKRTGRREKLPEWAIDQEAYLKKKALERANRQSKAPF"
BASE COUNT         1993 a         1465 c         1100 g         2657 t
ORIGIN      
        1 cagcacctgc cttcttacgc catttatttc tgtgcgctgc tagttcatct actggcttta
       61 cttctgggcg tgtaatttct tcatcttttc ttgcggtcga tcctccagca attagtctac
      121 cagtttttgc tttgtttgtt aatcctaata aatctagagc tttcatcttc ttatctgccc
      181 aagtttctac ttgttgtgcc aatggatgtt ttgattgatt agtagctcct gatttattcg
      241 tgaatttttg cgtctccgga aatccttttt ccttccacaa taaatatttg tgttggtaaa
      301 tttcaaaaat atccaagtat gattcgatta atggatcaag agtgatggtg tacaaatcag
      361 acaaattcat tatttctaag atacgagctt tttcagcact tactttttca tcaataatcg
      421 ctttacgttg cgctttagtc gtcatttttg tatacacccc cctttgtttt ttgaaaaatt
      481 tgacctaacg atgcgcgtga ctccccgcta ccctatctcc ccacgcgaaa aaattttgaa
      541 aatgaatagg ggggcttcaa ttgaaatacg aaggaaaaac ttttttgtct tccgtttcgt
      601 tttcaacaat tggatgacat tttgaacata atagcatgag attgtttgga tcaagcttaa
      661 gcagttcgtt gtctttgatt ggtacaatgt gatggacgtg tgctctcttt ccaaatatga
      721 actgaccaca ccgctgacaa tgaccacctt ctctttcata aataaattga cgcatatctt
      781 tccatgcttg cgttcgatag aatggtttgt tctcatgatg ataaacagac tttgcttgct
      841 gcttcttctt gcgtgatctg cttgatctct tgtgttcagt acagtagata ccctttgcta
      901 ttttgttcgt gcatccgtca aactgacagt atttcattct acttcacgaa taagatttat
      961 gatatcgcct tttgcacgga tagcaccagg aatatcaata ccatgtttct tagcatatgc
     1021 acgcaattct tttgcagtca tattgtccag ttcatccgta tcttcagtgg actgatcatt
     1081 agtagcttcg tctccatcaa aattagcagc agtgtttcca tcgctatcaa gaatttcact
     1141 gctatgagct atgagttcgc cattgacagc aacaaatgat ttgccatcac tcaaaattcc
     1201 tttttcttct tttacttcaa aatttggttc ttgatctttc ggaacaacta ctgtcctttt
     1261 cttttctgaa tcccaatact ctgttccact aatggatgtt ctaattttga tcattgccat
     1321 tttaattctc tcctttgtaa tttgtacgga ttacttttgc gcccattcgc tcataccatt
     1381 caatcgtttc ctgcagattg ggtagagtgt gtgacaataa agaaataact aacgtattca
     1441 ttccagttct gtaatcatca ttctccactt gcacatgtgc atgattacca ttccaatttt
     1501 ctctgtacgg actattctct gcttcaattg ctttcttata aactttagcc aaacttgatt
     1561 ctacttcaat actaagtacc gcttcatgaa aatcattcat ttgtttactc tcctatcaga
     1621 tattttgtgg tatgatttat aaaaaagatg cgaggtgttt taatgcttca agaagttaat
     1681 attaagtatc gtgatcgtca aaatacagag acaatttatt tgccaaatag ttgtccgcat
     1741 tgcggaaata taatgactcc gtatgtgtat tttggggttt catcatatag tgcgttcgat
     1801 aatgatagaa tctttggcgt attagtacag tgtataaatg atgagtgcaa caaattttat
     1861 agtttacaat ttataaatac gtttaatgaa gatgaaggat attctgaaaa tattttaatt
     1921 aaagattatt accgtccgcc gattaaaata gatttaccag aaaatattga aaaagtttct
     1981 cctacatttg ttgatattta tactcaagcc acacaagcta aatctgagca attggaacaa
     2041 attgctggag ttggttatag aaaagctcta gaatttctaa ttaaagatta tgcaatatct
     2101 aaaaattcag aggatgaaga taaaataaaa aaaatgttat taggtaaaac aattgaaaat
     2161 tatttgaatg attttcctaa actccaaaga ttagccaaag ctgctacttg gattggtaat
     2221 gatgaaacgc actatgttcg tagacatact aataaagatg ttcatgatat ggatgcattt
     2281 attaaatcag cagcgcaatt tatcgcggct gattatgatg ctgatttagc tgatcaattt
     2341 ataaatggaa ataactttat gccaactact aattaaaaga ataaaagagc cacagatacc
     2401 aattaataaa acggttggga tatcttggct ctttcatatt tttttgacac taatagaata
     2461 tcatgctgct aacaatgttt acatagctac aatgaccgaa catttagtga acagtgtaat
     2521 tttagaataa agagagttgt tctttgtact tctcgctatt gtaaaaatca atttcgtttt
     2581 cttttttcaa tcggtcagct tgtctgtgtt catactcatc taaaaagttc aatgtctttc
     2641 taatttctgc atgtcgctgt ctgacatacg atgagctata cccaacagat tcagcaatat
     2701 cttctaagct catttgatca acatatttca tcttaacaat ttgattatcg ataccagaaa
     2761 agctatctat cagcaataac atttcttctt tttgctcgat caacaattcc aattcatttt
     2821 ctatcttttg gatattctct tctaaagaag aagttctcga attcttttcg atgcggacgt
     2881 ttgctaaatc accacagacc catcgattca attcaagctt acttttatta agattccact
     2941 ttaagtaaag aatctgctca tctagttctt ggtaatcttt taaccaatga aatctcacaa
     3001 acgccacccc ttatggtaga atagtattgc gctgctatct cgaaaggggt agctttttta
     3061 ttttttctta gccgttccat tctggattgc cttttgttcc aaacgtcgtt ttttcttctt
     3121 aatctttgat tttgtttttc ccatacaatt ctcctttccg aaaacacatt ttgttcgatt
     3181 tttaatacta aataaaagtt ggccagaatt tttcgttcgt ctttactcat ctttttttga
     3241 ataagattac tagcataaaa atagttagta ccacgaaaat gaacaggtaa atcaattacg
     3301 ttcaccttct tcatgccagt tatcagccac tagaatcttt gcgttagtgc cgccaccgtt
     3361 ttcttttttg tatttttcaa atgctttata agcagctgtt tttgatttat agatttttcc
     3421 ggaaccgact gggaacatcc atgtgccctc tgtgatagct aagatgtata ctttcattta
     3481 attcacccct ttaatcccca acactacata tccggacttc tgcatgtaat cagtaatata
     3541 agtgatctct gctttgtatg agtcaccagt gtaactaaat gttggtctcg ttagtccagg
     3601 taattcatgt ttaatgtatt ttttatattc acgtagtatt aactgatcac caactttgaa
     3661 gtcacgatca ttcttgcgga tctcgaattg tttgcgtccg ctcgtcactg cttcaaaata
     3721 ttctggtagt atttttagtt cgtggatcat tctgtttcct ccttcaaaat tcgctcatca
     3781 tcaaaaagtg aatctccaca aaatccttct cccaaatatt tatcgttcat gtataagtca
     3841 accaaatatt tgtgatctgc ttctcgatat tcattaatat taacgatctt tattttgtaa
     3901 acattatctt cgtcgctcac tatcttgaat tgttctctga ctttcaccac cgttcctcct
     3961 ctttttgaaa aaattttagt gtttttgtaa gaccccctaa atcagctttt ttatttgata
     4021 taatacttgt atcgattaat caaatcgaaa ttaaatttaa aaaggtgatt tatcatgaat
     4081 atcaacaatt tagtaaaact cgttgaacaa cgttttaaag aatcaggatt agaacaatta
     4141 aaaaaagata attctccaga attattttct aattttaccg aagacgacat tcctactctt
     4201 aaacaattaa aatattattc tgattattgg acgcaaactc gtgagaatga attaaattta
     4261 gaaaaactca ttattaaaaa gaaatagcaa ctccagccca cacggctggt tttttagttt
     4321 caacttccct ccttacgggt caaaatcatc ggttccggca taatctgatc atcctttagc
     4381 aacaaatcag gatatttttt ccttagcttc ttattgttcg tgtaagtttt caatagccat
     4441 tggttggctt cgcttttgct ttctccagaa aaataaattt tccctgtttg gatgcctgca
     4501 agttgaatca tcattttcac ctcaaatcaa atttttttct gctagatgga gcaaaagcaa
     4561 tccgacatca agaatctttt gacccattat ttcagctact ttgctcggct taactccttg
     4621 agaaaagagc aagcgtgctt gctctatctc ttcgtgagtc caaataaatt tggcttcctc
     4681 aaggataatt actggatcag ttgctctcat tgttcctctg gaaaaatgat gtcattctta
     4741 tgcttggacc aggtatctgc atatggaaca aagtattgtc tagctaattc gatttgatca
     4801 attaaagcag tttctgataa ttcatggtct gcagcaattt cattcatact atcgccttca
     4861 tctaaacaga taagaccgtt gacgttatcg atttttacat ttgtgactgc ttgactttct
     4921 ccacgctcat aatattctac agttgatact gatacatcag cttcggagtt taattgcaga
     4981 atctgttact gccgggataa tttaatattc ttttcataat tcaccttttt tcctactgac
     5041 atcgaatatt tttcatgtta tgattagccg tgaaaggaga gctatattat gtgtttacac
     5101 atgatgtcct tagctcattc ggttctttgg aagctttttt ggaaataact ctcaggttct
     5161 tgacgcaaat ctcatttatt ctttgaaaca aaaaacttcc attttccgag agctttggat
     5221 cacttcaaaa gagatttgca tttatatttc tgtgaccttg agtcgcgtaa agaaccttac
     5281 agcaatggcg gcaattccac tttcaggaaa ccgccttttt tcattgatat tggtggttag
     5341 cggactaccc attttctttc ggcagtgcca aaccacaaat gatctgtgtt gatgtttagg
     5401 tagttgtata ccagataagc taaatccaaa gtgcctgcat cttcaagacg acaatattcc
     5461 aaaaattctc ccatagctgg acataaaaat cctggagcta tcatatccca acagttaata
     5521 gtgcaaagaa attcgtttag cttctgaacg ttttctttgg tttcacgaat tacaacatac
     5581 tccaattctt ctccgctggc agagcattcc aaaacttcaa attgattaag aaatggtaga
     5641 acatatgctt caaacatcaa cattccatct ttctgttttt gcacttgttt tgcatcaatc
     5701 atgtctatcc tcctattcag ttatttcttc cattaatgga attatttcag gtcactggac
     5761 ttaacgaaaa caccattgac catctttcca gtccgacctt tgatttcgtc ataagcaaaa
     5821 tccaagcact cgtataaatc catatcattt tgcattgcca agatgatcaa ggttactact
     5881 acatcgccaa ttccgtctct taaaccatgt tcatcttttc tagctagaga agcggcaact
     5941 tccccgattt cttcgaccgt ttttaacatt tgcttgctga aatccgcttg atccaatccc
     6001 ttatcttttg cccactgctc tactttcgcg attagttcgt ccattatttc tcctccacat
     6061 acctaaactg tcttcctttt gaatcaatcc ataagctcct agctctatcc caaataatat
     6121 ttttgctcag accagtaatt tcagataact gtttagcggt acccgttact agaattcgat
     6181 caccatgcca gattgcaatc tttctcggcg ttctccgttt gggcttttca gtccacattg
     6241 atttaccgag cttttggact tctgcaacta tttttttatc ttcctgccaa tcttctgaat
     6301 aagtcaattc gataattcgc tgcattgccg ctttcttatc catcccgaca ttctcctttc
     6361 agtaatttga gtacttgatc aagtgcgctc tcacgtccgc catgaaatgt gttgagccac
     6421 ttgtcttcgt acgacacact ttgtcttaaa gcttcttgat gcattaattc gatctgtgct
     6481 gtaaatgtct ttaggtccat cttgtttaca cctgctcaag ttcattaaga tgtttttgca
     6541 agcctttaac acaatcaatg aatagtaatt tggtatatgc taaatttctt aattgcgttc
     6601 catcgatata gagtgcgaaa tagtatctga gtttactcca actcgaacga tcattcttaa
     6661 ttccttcaat tccagcttct tcgagttgat catatacgtc tctcaggacg tctaattcct
     6721 cgcccttttt ataattcgct atttcattaa ttagttctag ataatcgatc ttcatttttc
     6781 cacctcttag aatggtgctt ttgattgtct attagctcgt tctagcgctt ttttcttgag
     6841 ataggcttct tggtcgattg cccattcagg aagcttctct cgtcttcctg tgcgcttgta
     6901 tccactgctt gcgttcttag gttcactttt ttctttcctt gcccaacttc gaatagttgc
     6961 caaatagttt ttataagtct taccagatga ttcacaatac tctgacagtc gttcgattcg
     7021 ctcttggtaa tcattaggga attctgtttt gagtttctcc atctgctcat ctgacaaaag
     7081 aacattttta tactctccgt atttatgacg gacgggctta gccttcgatt ttttcgaagg
     7141 cggtaactct cttatatatt cttttgtatt attaaatgta ttattaatag atgtattatt
     7201 atctttgact ttttc
//
LOCUS       DASHRQ010000097         7210 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.102, whole genome shotgun sequence.
ACCESSION   DASHRQ010000097 DASHRQ010000000
VERSION     DASHRQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.102"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..222)
                     /locus_tag="U2M88_002591"
     CDS             complement(<1..222)
                     /locus_tag="U2M88_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEM8374919.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWK"
     gene            368..451
                     /locus_tag="U2M88_002592"
     CDS             368..451
                     /locus_tag="U2M88_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEM8374920.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            576..1313
                     /gene="erm(B)"
                     /locus_tag="U2M88_002593"
     CDS             576..1313
                     /gene="erm(B)"
                     /locus_tag="U2M88_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEM8374921.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1483..1743
                     /locus_tag="U2M88_002594"
                     /pseudo
     CDS             1483..1743
                     /locus_tag="U2M88_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1906..2571
                     /locus_tag="U2M88_002595"
                     /pseudo
     CDS             1906..2571
                     /locus_tag="U2M88_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2607..2852
                     /locus_tag="U2M88_002596"
     CDS             2607..2852
                     /locus_tag="U2M88_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEM8374922.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2955..3824
                     /locus_tag="U2M88_002597"
     CDS             2955..3824
                     /locus_tag="U2M88_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEM8374923.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3805..4539
                     /locus_tag="U2M88_002598"
     CDS             3805..4539
                     /locus_tag="U2M88_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM8374924.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4572..5484
                     /gene="ant(6)"
                     /locus_tag="U2M88_002599"
                     /pseudo
     CDS             4572..5484
                     /gene="ant(6)"
                     /locus_tag="U2M88_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5481..6023
                     /gene="sat4"
                     /locus_tag="U2M88_002600"
     CDS             5481..6023
                     /gene="sat4"
                     /locus_tag="U2M88_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HEM8374925.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            6116..6910
                     /locus_tag="U2M88_002601"
                     /note="aph(3')-IIIa"
     CDS             6116..6910
                     /locus_tag="U2M88_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEM8374926.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT         2509 a         1097 c         1487 g         2117 t
ORIGIN      
        1 tttccatgaa taaaaggatt ttttattttt ctttttccaa atttgataga gtagtttgcc
       61 atattcttgc acccaacgat aaatcgtcgt atgagaaacg ttaatgccac gatcatataa
      121 gatttcttga acttcacgat agctaaggtt ataacgaaga tagtagccca cggctacaat
      181 aatcacatcc tgctgaaatt gctttccttt aaaatgattc atcgtcattc ctcctgctat
      241 ctttttctat tattctacct tatttgatag tagatttaaa actttgcaac agaaccattt
      301 tgtgtataat aggaattgaa gttaaattag atgctaaaaa tttgtaatta agaaggaggg
      361 attcgtcatg ttggtattcc aaatgcgtaa tgtagataaa acatctactg ttttgaaaca
      421 gactaaaaac agtgattacg cagataaata aatacgttag attaattcct accagtgact
      481 aatcttatga ctttttaaac agataactaa aattacaaac aaatcgttta acttctgtat
      541 ttatttacag atgtaataac ttcaggagtg attacatgaa caaaaatata aaatattctc
      601 aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg aatttaaaag
      661 aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg aaactggcta
      721 aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac ttatcgtcag
      781 aaaaattaaa actgaacatt cgtgtcactt taattcacca agatattcta cagtttcaat
      841 tccctaacaa acagaggtat aaaattgttg ggagtattcc ttaccattta agcacacaaa
      901 ttattaaaaa agtggttttt gaaagccatg cgtctgacat ctatctgatt gttgaagaag
      961 gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg cacactcaag
     1021 tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa
     1081 acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa tattggaagc
     1141 tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg tttactaaaa
     1201 atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc gttacttatg
     1261 agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa taattctatg
     1321 agtcgctttt ttaaatttgg aaagttacac gttactaaag ggaatggaga taaattatta
     1381 gatatactac tgacagcttc caagaagcta aagaggtccc tagcgcctac ggggaatttg
     1441 tatcgataag gggtacaaat tcccactaag cgctcgggac cccttgtagg aaaatgtcct
     1501 aagtgtggca acaatattgt attaaaaaaa tcgttttatg gttgttcaaa ttatcctgaa
     1561 tgtaagttta ctttagctga acattttaga aagaaaaaac taaccaaaac gaatgtaaaa
     1621 gaattactgg agggaaaaga aaccctggta aaaggaatca aaaacaaaga gaaaaagccc
     1681 tacaatgccg ttgtaaaaat tggggaaaag ggatatattg attttatctc tttttcaaaa
     1741 taaacatggg gctgacccaa aagtagctcc tatatgaaaa atagaggtct ttccatttag
     1801 atagtagtgg aaagacctct atttatcttt tgaaagtgaa tacaaaaata aagcacactc
     1861 tgctataata taggtgatta atccatttga gagaggtgct ttatcatgta tcaaaattat
     1921 accataaacc aactttgttt gcctatagat cttgaaatta aattagaaga aaatgatttt
     1981 gctcatgcca ttgtgcagtt tgttgactct attcccgatg aagtctttct tccttattat
     2041 caatctatgg gtagacccca atatcatcct cgtatgctac tttccattat tctttgtgcc
     2101 tatattcaag gggtttattc gggccgccaa atacaaaata tgctgataga tagtattcgg
     2161 atgcgctact tatctcaaga gcaattccct aactttcgta ctattaatcg ttttcgcgtt
     2221 catccgatta tgaacgatat tctagatcat tcatttgttc aatttcgtga gctactggtt
     2281 caaagtggct taatcagtgg cagtgctcta tacattgatg gtacaaaaat tgaagcggat
     2341 gcgaataaat actctttcgt ttggaaaaaa agtattttaa agtataagga tagccttgat
     2401 caaaaagccc ttgaaaacta ccaacaaatg gtggaaacaa aaatcttacc tgagttaata
     2461 gatgatttaa aggatgaact ttctatagaa gagattgaaa ggattcgtca atctttggaa
     2521 gaaaaggaaa atcaattaat tgaagcgatt gaccaaaccg aaacagtaga agggggctga
     2581 cccattatta ataaggagtg gacaacatga ttgatgtaaa tgagctattg gaagaagcta
     2641 ttagagaaac tgaaaatcta aatgatggcg aagtttttct tgttaaagat ttattcaagg
     2701 gatatgtggg gaataggata cccagaaagg atcgacttct gcttggaacg ttatttttaa
     2761 accatgttag caaaatgaat ggtaatttaa aggctattga aaagacttca tctaatcagc
     2821 agaggtataa aaagacaatt gataagtttt aactatcttt tgtgaaatta aaaaaattat
     2881 aaatatgttg agtagaagca aaagatttgg cttggaattt ttacagcagt gaatcgataa
     2941 aaggagcatg cttgatggta gataatatta ttaaatcagt agcagagaaa ttatcctctc
     3001 tgtcttatat agaaggtatt gttttaggtg gttcacgtgc aaggggcacc catacagagg
     3061 attcggatat agatatcggc atctattaca attcagaatc atttgacata aatactatta
     3121 atcaattcgc tacaaagctg gatgatgagc atagaaataa ccttgttgta cctcccggag
     3181 catggggtga ttggattaat ggcggcggat ggttagtcat aaacgggtat catgtggatt
     3241 taattttacg tgatattaaa cgtgtggaac aaataatgaa agatacagag cacggaattg
     3301 ttactgccaa ttatcagact gggcatcccc atggttatat tagtgcaatg tatcgaggag
     3361 aattagcgat tagcaaaata ctatatgcta agaatgaaag cttatgcgaa ttaaaaaaac
     3421 aggcagaaac ttatcccaat gctttgcaga aaagtttagt taactttttt atgtttgaag
     3481 cagggttctc tttaatgttt gtaaaagcaa attcgggaac agacgataaa tattatattg
     3541 cgggtcatgt ttttcgtata gtttcatgtt taaatcaagt gttatttgca tgtaataatg
     3601 cttattgtat caacgaaaag aaagctataa aactgcttga aacttttgaa cataaacctg
     3661 aaaaatatac cgagaaggta aatcatattt ttgaagtact cggtatctca ctttttgaat
     3721 gctacgacat gaccgagaag ctttataatg aagtgaatga aattgtatcg gagataaata
     3781 actttttaaa cgaggagagt tcagatgaaa gaaaacaaat atgatgataa tatatttttt
     3841 caaaaataca gtcaaatgag tcgctcgcag aaaggactgg ctggtgcggg agaatgggag
     3901 actttgaaaa agatgctacc tgattttaag ggtaagcgtg tgcttgattt aggatgcggc
     3961 tatggatggc actgtatata tgcgatggaa aacggtgctt cctctgtagt aggtgttgat
     4021 atttctcata aaatgctcga agtagcaaaa ggaaaaaccc attttccaca gattgaatat
     4081 gaatgctgtg ccatagaaga tgtggatttc ccagaggaga gctttgatgt aatactaagt
     4141 tcgcttgcgt ttcattatgt agcagactat gagaatttaa taaaaaagat atataggatg
     4201 ctgaaggctg gtggcaattt agtttttaca gttgaacatc ctgtttttac tgctcatgga
     4261 acacaagact ggtattataa cgaaaaagga gaaatactgc atttcccggt ggacaattat
     4321 tattatgagg gcaaacggac agctatgttt ttggaagaaa aggttacaaa atatcataga
     4381 acactgacca catatctaaa tacactgctt tcaaatagtt ttataataaa tcagattgtg
     4441 gagccacagc cgccagagaa catgatggat attccgggga tggcggatga aatgcgacgc
     4501 ccaatgatgc tgattgtatc ggcaaaaaag aagatgtaat aatatagaaa aaataaacga
     4561 ggagtatgta aatgagatca gaaaaagaaa tgatggattt agtactttct ttagcagaac
     4621 aggatgaacg aacgtattcg aattgtgacc cttgaggggt cacgcgcaaa tattaatata
     4681 cctaaagatg aatttcagga ttatgatatt acatattttg taagtgatat agaaccgttt
     4741 atatctaatg atgactggct taatcaattt gggaatataa taatgatgca aaagccggag
     4801 gatatggaat tattcccacc tgaagaaaag ggattttcct atcttatgct atttgatgat
     4861 tacaataaaa ttgatcttac cttattgccc ttggaagagt tagataatta cctaaagggc
     4921 gataaattaa taaaggttct aattgataaa gattgtagaa ttaaaaggga catagttccg
     4981 actgatatag attatcatgt aagaaagcca agcgcaaggg agtatgatga ttgctgcaat
     5041 gaattttgga atgtaacacc ttatgttatt aaaggattgt gccgtaagga aattttattt
     5101 gctattgatc attttaatca gattgttcgc catgagctgc tgagaatgat atcatggaag
     5161 gtcggcatcg aaacaggctt taaattaagt gtaggcaaga actataagtt tattgaaagg
     5221 tatatatccg aggatttgtg ggagaaactt ttgtccacct accggatgga ttcctatgaa
     5281 aacatatggg aagcattatt tctatgccat caattgttca gggcggtatc cggtgaggtg
     5341 gcggaaaggc ttcattatgc ctatccggag tatgatagga atataacaaa atataccagg
     5401 gacatgtata aaaaatacac tggtaaaacc ggctgcctgg atagcacata tgccgctgat
     5461 atagaagaga ggcgggaaca gtgattacag aaatgaaagc agggcacctg aaagatatcg
     5521 ataaacccag cgaaccattt gaggtgatag gtaagattat accgaggtat gaaaacgaga
     5581 attggacctt tacagaatta ctctatgaag cgccatattt aaaaagctac caagacgaag
     5641 aggatgaaga ggatgaggag gcagattgcc ttgaatatat tgacaatact gataagataa
     5701 tatatcttta ctaccaagac gataaatgcg tcggaaaagt taaactgcga aaaaattgga
     5761 accggtacgc ttatatagaa gatatcgccg tatgtaagga tttcaggggg caaggcatag
     5821 gcagcgcgct tatcaatata tctatagaat gggcaaagca taaaaacttg catggactaa
     5881 tgcttgaaac ccaggacaat aaccttatag cttgtaaatt ctatcataat tgtggtttca
     5941 aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg
     6001 ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat
     6061 aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc
     6121 taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga
     6181 tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata
     6241 tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga
     6301 catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca
     6361 tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta
     6421 tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt
     6481 tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga
     6541 attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga
     6601 cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga
     6661 ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa
     6721 agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc
     6781 cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt
     6841 tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga
     6901 attgttttag tacctagatt tagatgtcta aaaagcttta actacaagct ttttagacat
     6961 ctaatctttt ctgaagtaca tccgcaactg tccatactct gatgttttat atcttttcta
     7021 aaagttcgct agatagagtt ctatattaga gatgtaaaga gttttggtga agaggtttgt
     7081 gagtatggct tttataacca gggtgcaatg agaagcacta acagcagcta gcttaagaac
     7141 gcataggata tactttttat ggaagtcaca gaggaggaat aaaatttgaa caggattaac
     7201 agagtaacct
//
LOCUS       DASHRQ010000098         7066 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.103, whole genome shotgun sequence.
ACCESSION   DASHRQ010000098 DASHRQ010000000
VERSION     DASHRQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.103"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            237..1877
                     /locus_tag="U2M88_002602"
     CDS             237..1877
                     /locus_tag="U2M88_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEM8374927.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="U2M88_002603"
     CDS             2031..3353
                     /locus_tag="U2M88_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEM8374928.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="U2M88_002604"
     CDS             complement(3415..3846)
                     /locus_tag="U2M88_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM8374929.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="U2M88_002605"
     CDS             complement(3910..4746)
                     /locus_tag="U2M88_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEM8374930.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="U2M88_002606"
     CDS             complement(4936..5238)
                     /locus_tag="U2M88_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEM8374931.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="U2M88_002607"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="U2M88_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEM8374932.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="U2M88_002608"
     CDS             complement(5692..5958)
                     /locus_tag="U2M88_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEM8374933.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="U2M88_002609"
     CDS             complement(6212..6937)
                     /locus_tag="U2M88_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEM8374934.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
BASE COUNT         2083 a         1453 c         1258 g         2272 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaaga
//
LOCUS       DASHRQ010000099         7056 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.104, whole genome shotgun sequence.
ACCESSION   DASHRQ010000099 DASHRQ010000000
VERSION     DASHRQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.104"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..340)
                     /locus_tag="U2M88_002610"
     CDS             complement(<1..340)
                     /locus_tag="U2M88_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM8374935.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVR"
     gene            complement(1671..2336)
                     /locus_tag="U2M88_002611"
     CDS             complement(1671..2336)
                     /locus_tag="U2M88_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8374936.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2516..4498
                     /locus_tag="U2M88_002612"
     CDS             2516..4498
                     /locus_tag="U2M88_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEM8374937.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4502..5140
                     /locus_tag="U2M88_002613"
     CDS             4502..5140
                     /locus_tag="U2M88_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8374938.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            5168..5596
                     /locus_tag="U2M88_002614"
     CDS             5168..5596
                     /locus_tag="U2M88_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374939.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            6053..6964
                     /locus_tag="U2M88_002615"
     CDS             6053..6964
                     /locus_tag="U2M88_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEM8374940.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2424 a         1082 c         1303 g         2247 t
ORIGIN      
        1 ggcgaacata attctttcca aaagttgaga aatgggggat tttctcctcg aaggagtagc
       61 ctaagaacca gcggtaagcg acattggtat cgacttcctt aatggtttgt cgcatggaac
      121 ggataccgaa taaatactga ataaacacca atttgatcag aatcactgga tcgatacttg
      181 ggcgacctaa atcggagtaa gtggcttcga cgattggata aataaattca aaatcaagcg
      241 cggcatcaat ctttcgaacg agatgatctt gaggaactag atcatcaatc gtaagcatcg
      301 caaattgatt tctaccattc gtattgtttt tagacatcat agctatcact ccttgcattc
      361 gttttcataa atctattata aaagaaaaga ctgtagatat catccataaa atggactttg
      421 tctacagtct gaggactacc tgtattagtc ctcttttttg accagatttt gaccagtcta
      481 ttttacattc taatatattc atatgagcta attttatgat taaatggtaa gtgtttcctt
      541 gtttgtttta gtttaaacta gtgtgtaatt taaaaatcta tatggaaggg tctgtggggt
      601 aatacttaaa taaaggctag gaacctgata acatcaaggt tcctagcctt tttataataa
      661 aatgaagcga aaataatttc ttttaaagag ccgagattat cagctatata agagtgaatt
      721 tgatgcaaaa aacagagccg aaacagtctt ggtgttcgta gctctgctta tatgtttcga
      781 atttaagaca aagagttggc aaaagatgtt gataaggaag ataagataac tgtgttatat
      841 aaactaggta actaactgta acaagctaat tttaagagat tcttattaga agacgtttag
      901 gaatataact aagaaaatga ttatacaggt aattgtcatt tcaataatgt tttctaattt
      961 tcgcgtcttt gttttaagaa aaaggccaga atcagttgga aaaaagttcg tacgatagtg
     1021 aagaggggca aaatttccta ctaatattcc cactaacaag agataaaacg gccaggttgg
     1081 tgtgattcct ctataaaaaa atgcccatgc taccgaaatc ccatagatta acaactgaac
     1141 aatttttaca atgaaaaaat tgttctgcca ttactcaaag tctcttcctc cttgtatcgt
     1201 ttatttgttt cattttatca ttatgatagg cgcttgaaca atccattttg tattttattg
     1261 agtactcttg gataaactac cagcgattat catatttatt tatgttactg taagccatca
     1321 tataaaagta aatgagcaga tcatagaaat ataaaaaaga actaaaattg atctaaatta
     1381 tgtgcagctt gactacgtac caatctatat tacttactag attggtcgtt aatctattcg
     1441 gatcgaatga gcaaaatatt tttgtgaatg tccatccttg attatgattc caaaaagaat
     1501 cattgggagg ctgtggcgta aatattatac tataagccag aaatacctat cattctgaac
     1561 ttttttattt aggaactttc atctcaaggc tttttcaatt aatttgtacc cctgtttttt
     1621 ggataagatc atgtgcattg attttttcta tctctacgtt catctcctac ttaatgccta
     1681 tctggctcct gagggcttct ttttaaggcg tcgtcatagt cttggctgag gatttcttgg
     1741 taatctgagg gaatggcttc ccaagtgagc aggcgaacgt tcatgacgtg ttttcctttg
     1801 tgtttgatta cagcatggtt tttctctttt tctacctctc cagcgtgaaa agacagccat
     1861 gtaccatggc caaagatatc attacatacc caacgttcga tggaattgtc cacgtatcct
     1921 acagggagtg tggtatagcc gatttgtggt ttcaaaaaag gattgaatcg atcgatcgtt
     1981 tcatttttgt attgataatt gataagaaaa agatagacca aacaaaagac gagcagaaag
     2041 aaagatagtg tctttttgag tgagaagggt tgttttgatg aataagattt cttgttaaca
     2101 agaggttcat ctggcaatgt tggttctatt gattcggtag ctgattcaat agaaggaaaa
     2161 tcaatcggca gacctaaagt atgatgtaaa agtgacagca tttcaaggtc tggcattgta
     2221 cgagaatttt cccaactaga aatcgtttta tcggaaacac caattttttc gcctaattct
     2281 ttttgcgtaa gcttacaatt tctgcggtaa gttcgtattt tttctccggt gttcatcttc
     2341 ttctcctttc gattctacag tatatagatt tattctacat gacctataca cttgatagat
     2401 acgttttttt gatgaaaaac ggtatgattt tatcaaagaa tcatttaagt aagggagagt
     2461 ggtgataatc aaaaaaaagt tcacagtttt actcacaatc atagtgaccg gtatcatgag
     2521 tggttttgcc ttggttagta taaataaaca ggtactctat cggacaaatg aatttttcca
     2581 tcaaacagat acttatactc cgattcgatt gccacaacat tttattgggc aagaagaata
     2641 tgagcagcta atcatggtat ttgaaaatgt ctcatcagaa actgagcttt cttatatgaa
     2701 aaaaacagta aatgaatcca atgaagtaga tacatcttgg aacttcaaga tacagccaaa
     2761 gcttgaaatg acattgtatt atcaagggga tgtcaaatct tctcacgaga aggtacccta
     2821 ttctaatacc tcctttcgcg taacgtccat tgaagaatcg atcaaaataa aagattttga
     2881 aggtgaattt ttcttgatga cgagagaaaa agaagcctat gaacggttca tttcactatt
     2941 cactgatcga ttcaatcaag catttcacac tgattatgcc actaatattt ttgaagactt
     3001 tgaaaatagc tattcgtatt cggttatttc aaatacagac gtatataatt atcgagtgta
     3061 tttaactatt ggattgattc ttttattcct tttgttatca tcctttttct tattgatgaa
     3121 taaagaaatc agtttattgt tgaataatga cttttcatta catcaagtat tgtggtgttt
     3181 attggggaaa aagaaaatca ttattacatt cataattgca ggaatcattg gtggttttat
     3241 ttttatgtat tataaggagc aatttcttta tttgttccag atgtacgttt tcttgttcag
     3301 cggaatatat ggtatctctt tgatttctct tttattagtt gaaaaaatcg cttcgttaag
     3361 agaagcgttc aactatatcc aatacattct ttttacattt ggattgttgt tttctcttct
     3421 tttgaccgtt gccaatgtcg aattagcaac tatcgttttt tcttcttcac agttagctgt
     3481 ttcccatgaa ccaccaaaag aaatcgctgg tgaggactat catgtatttt atccagtaac
     3541 catcgggaaa aaccacgtgg attttattta ttccaatcgt tttgcatcag cagcagatca
     3601 ggaattatat gaaactttga ataaaaacgg aagtatatta gtcaatgtat cagattactt
     3661 gaatgaagaa aatgaacagt ttggcagagg gataaaaata aatctgaatt atcttaagaa
     3721 atttcctttg attgatgtct ctggaaggtt gatccaaata accgaaaaag aaacgcaccc
     3781 tgttatattg atccctgaac gatatagaca tacagattta aaaaatgacc tggcgcaaat
     3841 aacagaatat tatcaagaaa tcagtgaaaa agaaccgaaa tttctattta taaaaaacaa
     3901 tcaacccatt tatacattta ttccttctat cccctggatc gaacattatc cggttgttga
     3961 aatactgaca ttgaaaaaca gtatgtattt ggaaagaaat attctcagcg gagaaatcta
     4021 tccaccgtta aagatcaaga taggctcttt gtcgaaagaa aggttatttg agctgattga
     4081 agaaagtcag ttacgtgata acttgcagtt atcatttccg tatactcaga ctgaagagat
     4141 agctgtcaaa gtgttaagca gatcatttca ttacttgata cagatatttt tactcacttt
     4201 cttttctttc ttcttgttga gctatgtcat gttatgcata tattttgtct acaattgtcg
     4261 gaaaatcgct atctttcgtt caagtggtta ttcgctcttt gaaacgtata aagatttctt
     4321 catgatgaac ctcatcaaat ggggaacgac aagtgtaatc ttcctttttc ttatagaaag
     4381 agagcctaaa tatcttttta atattttttt ctttagcttc attgatttta ttctttcagt
     4441 tgtttttatt ttgtctacag aaaagaaaag tcagttattg ctgatgaatg gaggttaaca
     4501 attggagccg ttgatagtga taaaaggtgc gaaaaaaatc attcgaaaca aaacgattct
     4561 tcatttggat cgtttcactg cggatgctgg ggatcttgtg actgttgttg gagaaagtgg
     4621 agcaggtaaa agtacgttat tgaatatatt aggattgaat gatcagttca gttcaggaca
     4681 attctatttg ttcgatcaag agacgaaaca attaactggg agagagaagc gcttgataaa
     4741 aagaaatacc atatcttttc tttttcaaga ttttgggttg attgaatatg aaacaatcga
     4801 ttttaattta gatattggat tgaagttttc gaatttaaat gtttcagata agcgaacagc
     4861 aaagtatgag gcattgaaaa aagtaaatat aaacaagaag ctaagtacgc ctatctacac
     4921 attatctggt ggtgagaagc aaagaatcgc ctgtgcgaga gtcttattga agaatagcaa
     4981 aatcatttta gccgatgaac cgactgggtc attggataaa agaaataagg ataaagtagt
     5041 tgaaatctta tcgcaacaag cttcccaagg tacgttagtt atcgtagtca cgcatgatcc
     5101 atatctgacc aaaataggaa acaagaagat catgctttaa aaatcaaaaa ggaaaggatg
     5161 tcgttttatg aaaaaagcag ttattatttg tgcactaggg ataggttttg ttggaggaaa
     5221 cgctatgtca tctgaagtgg aagcaaattc agttcacggt gaaatctatt acaaatcttt
     5281 aaagccagga gagattcatt ttgatgctaa aggtaatatt attaagtcaa atttaccagt
     5341 agaccgggaa atttctagaa cagcaattaa tcatagctct ggaagatggg tttattatag
     5401 tgatacaact tgtacgagaa aaacaggtca ttctaaccac tatagtcgtg tctataatcg
     5461 tcacggtgca aaagcgaaag ttggaaactc tattcgatca gcaaatgcaa caaaaggagt
     5521 atgggcctat gcaagtgcga caggaaatag aactgacaca ttctcatgtt ggtacaatcc
     5581 aaccggttgg tattaagtat ccaaaagttt atataaaata gaaaaaacat ataatgaaac
     5641 agcgttaaaa gttatttttg gtagttttaa tatattcatt tttagttgta taaaaatata
     5701 tcataataaa aacaacaaaa aaatgtctgg aaaatcgaac ataaaagtgt ctgatttgaa
     5761 tatcccagca gcggaagaag aaaaacaaaa tcgataacat ttgttctaag aattagttac
     5821 gttaaatacg gaacgcaaga tagacttgaa aaacggtaac aaaagaagta gtatattaag
     5881 gtgtgatgtg ctacttgaca tttattctgc tgagcgaact ttctggattg tgtagcctta
     5941 tatctattca ggacgtatcg agcagattag taaaggtgtt cttgatgatt ttcatcttga
     6001 acacctttgt ttttagcaat tcgcaaaaaa cgaaaagtag gagaattgat aaatgaaaaa
     6061 aagattttta gcacttgcca tcgtactagg cactggctta ttgtcaggct gtacgaatgc
     6121 cggtgaaaaa acagccgtta gttataaagg cggtacaatc agcgaacaag aagtaatgga
     6181 tagcttgaaa aaaatgcaag gagcagattc cgctgtgcaa cagttgatcg tttatcaagt
     6241 atttgaagat aaatatggag atgacgtatc aaccaaagaa atcgattctc aatatgatca
     6301 aacaaagaaa caattgggtg attcatttga ttcacaattg aaatctgcag gttacacaga
     6361 acaaacattc aaagacagta tcaaacaaag tctagccttt caagaaggtc taaagaaaca
     6421 tatcaaattg accgatgaag atttaaaaac ggcttgggaa tctttccatc cagaagtgga
     6481 agctcaaatt atccaagtag caagtgaaga tgatgcaaaa gatgtgaaga aagctgctga
     6541 taagggagac gatttctcta aattagccaa agacaagtca actgatacta cgacaaaaga
     6601 agatggcgga aaagtgaaat ttgattccac tactacaacg gttccggcag aagtcaaaga
     6661 agcagccttt aagttgaaag atggtcaagt ttctgatgtg atcacttcta ccaatgcttc
     6721 aacttatact acggaatatt atgtggttaa aatggtgaaa aaccaaaaca aaggcaacga
     6781 catggataaa tacaaaaaag aattgaaaga aattgcaaca gacacaaaat taagtgacag
     6841 taccttccag aataaagtaa ttggcgaagt attgaaagac gctaatgtaa aaatcaaaga
     6901 caaagacttt gaaaatgttt tgagtacatt cacttcagat tctagtacag caagtagtaa
     6961 ataagcggta taaataaaaa atatgaacta tttaaatcaa taaaactgtt catctaaatc
     7021 tttttattag aactaaatca acagtagttt aattac
//
LOCUS       DASHRQ010000100         6706 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.106, whole genome shotgun sequence.
ACCESSION   DASHRQ010000100 DASHRQ010000000
VERSION     DASHRQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.106"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..243)
                     /locus_tag="U2M88_002616"
     CDS             complement(<1..243)
                     /locus_tag="U2M88_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEM8374941.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(358..936)
                     /locus_tag="U2M88_002617"
     CDS             complement(358..936)
                     /locus_tag="U2M88_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM8374942.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1013..1543)
                     /locus_tag="U2M88_002618"
     CDS             complement(1013..1543)
                     /locus_tag="U2M88_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEM8374943.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1547..2407)
                     /gene="rlmB"
                     /locus_tag="U2M88_002619"
     CDS             complement(1547..2407)
                     /gene="rlmB"
                     /locus_tag="U2M88_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEM8374944.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2400..2795)
                     /locus_tag="U2M88_002620"
     CDS             complement(2400..2795)
                     /locus_tag="U2M88_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEM8374945.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2792..4204)
                     /locus_tag="U2M88_002621"
     CDS             complement(2792..4204)
                     /locus_tag="U2M88_002621"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEM8374946.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4201..4737)
                     /gene="cysE"
                     /locus_tag="U2M88_002622"
     CDS             complement(4201..4737)
                     /gene="cysE"
                     /locus_tag="U2M88_002622"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEM8374947.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4823..5062)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5059..6519)
                     /locus_tag="U2M88_002623"
     CDS             complement(5059..6519)
                     /locus_tag="U2M88_002623"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEM8374948.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
BASE COUNT         1709 a         1480 c         1207 g         2310 t
ORIGIN      
        1 aactgcttca gttaaaaatt cgatctcaac tgtccgagta agtacatctg aatagctgta
       61 agatactcgt tcaaaagaat tttcttcagg atccaaatca acaacgaata ctgaaggata
      121 ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt cttcctgtct gagcaaccaa
      181 catgattggg ctgccgatac gaccttctaa atctttcttg attgaagcta aagttgttgg
      241 catacagttc acctctacta tcaactatac cacatatttt taaaattttc aagtttacca
      301 taaaaaaaaa agaaatgcaa taaaaataaa taagaagctc attaagattt ttctaaatta
      361 ttcctcatta taaaatttca agtgtttttt tattttgagt tttactctcc cgtaagcgct
      421 cattacttgc ttgtgatcca ttgctaaaca ttcagccatg tctacaaaat caacgccttg
      481 catataataa tgaaaaattt tagcttctat ttttgaaaac tggatttcgc aattttcgat
      541 tgcttctttt aataaaagat gctgaatcgc taaaggttct tgcaatgtga tctctgctaa
      601 aaagtctggt ccttctgtct ctattttttg gtcgattgag acttcatctt tttgatcacg
      661 acgcttcagc gcgttctgct tcctcaacag agaatagatt tgattgctta taatgctttt
      721 ataataactt ccaaagctaa cacctttgct tttttcgtaa gttctcaatg cctgatataa
      781 agcgatcctt gcttcttgtt cccaatcctc agaaacataa tttcttaaat aataggtgtt
      841 tttcaaacgt agtaaaagtg gaaaatattt ttgatacagg gcagcaaatg cttcttcgtc
      901 accattacgt gcagcgcgca ataactcctc agacataaaa taattcctcc ataaattcgt
      961 ttatggagga atcttatcat caatgattca tcgaactgat tagaacgata tgttatttat
     1021 tttttctcat taaatcgtcc aatttttggg acaatttttt taactgctct tcattccatg
     1081 gagaattacg tcggaaatca ataaaatgca tatctttatt ttcttgggct attgtttttt
     1141 ccgttttttt tatcgtctta tataattcat aagcagaagt tcgtaatgct ccttgggaaa
     1201 agatcatcca ctgttctgct aaatcgcttg tcgccactgt cacttgtgtc aaacggtcat
     1261 tcagctgacc agcgattctc tcgatataac tgtcagccgt ttcgtcttct ttcgtaaaaa
     1321 caacatccaa ctggtatttc ttataatttt gttggatgcc cggaactagt tgggcatcga
     1381 agacaacgat gacttccatt ccttcatatt tcgcataatt tgataaacga tgtaaaagag
     1441 cttctcttgc atccgccatt ttgtcctgct ttttaagctg gacaagttcc ggccacgaac
     1501 caatcatgtt gtagccgtct acaatcaaca gctgtttttt catggtctac tccccctgac
     1561 gttgggaaaa agcctgatac ataagcaaac cagcagcaac actggcattt aagctctgga
     1621 cgtgcccggt catcgggatc gtcagcaatt catccatttc ttttttcaat cctggactca
     1681 ttcctttgcc ttcattacca atgactaaag caattgatcc tttggcattc catttttgga
     1741 agtttgtgcc ggtcatatct gtgccaaata cccaaaatcc tgtttctttg agctgttgca
     1801 cagcttgtac caaattcgtc acgcgtgcaa cagggacatg ttcgactgcc ccagtggaag
     1861 tctttgttac gataggtgtg attccaactg ctcggtgttt tggtatgatc acaccatcga
     1921 cgccacttgc gtctgctgtc cgtaaaatcg acccaaaatt gtgtggatct tcgatgctat
     1981 ccaaaatcaa aaagaagggg tcttcttttt tctcttttgt tcgttcaatc agttggtcaa
     2041 gggataaata ttcataaggt gtaatcgcaa gaacgactcc ttgatgcacg ccgtgatcgc
     2101 tcaaagtttc taatttttgt ttaggaaccc atttgactgg cacagcttgt tcacgtgcga
     2161 tttctttcag ttcttctact ttttctcctc gtgaatcttc ttgaagaaag agtttattcc
     2221 ctcgcccttg ttgtagcgct tctactgcgg catggtgccc aaagacgaaa ttatcttcta
     2281 atgcttcttc attttgatca gaaactgact gatttcgtcc agtttgcttt ttatttgaat
     2341 atctattttg ttttttgtct tcaaatccac gtttcgattg atgtttgttt gttctttctt
     2401 tacccatttt tttcaccaac tttctgaata caccagcgga tcaattcttc catccgctcg
     2461 gtttgttttg tcaaatgcag atagcccatg agcgcttcga accctgttgc gacccgatac
     2521 gtcgtaacat cagcattttt tgcgctcgta tggcttttag catttcttcc tctgcgataa
     2581 taaagttctt cttcttctgt caaaagatgt tcttccagca tttgctgaat caaaaaggct
     2641 tgtgcattcg cagaaacata attcgttgcc gcacgatgca gcttattggg ttttgtttct
     2701 ccttgatcaa tcaggtggtc acgaacatag atttcgtaaa tcgcatctcc aacataagcc
     2761 aaggctaaac cgttcaattg tgtgtaatct ctcatgcttc tcttctccat cgggttcctt
     2821 gcggtgtatc ttctaataag atcccttttt ctttcaacaa atcacgaatt tcgtcgcttc
     2881 ggacaaaatc acgattgctt cttgcttgat ttcgttcttc gatcaattgt tcaatctctt
     2941 catctaataa ctcttctgct gtaaataaaa taccgaagat cgaaagccat tgcgagaata
     3001 aagcttctcc ttgtgccaag acagcggcag aaactttcgg ctggtctaga taagtgttca
     3061 gccacttagc aagctcatag accactgtga tcccatttgc tgcattaaaa tcatcgtcca
     3121 tttcttcgat gaatcgctgt tctaaatttt ctaacaaagc gattttatcc gcatcatctg
     3181 ccaaattctc tgaggcagtt tcttggcgga atttcaaatt gtcaaacgta ttcttcagtc
     3241 gttgtaaatt ggtggctgct tctttcatcg tcgtttcact gtagcggatc ggacgacggt
     3301 attgtgtggt cgccatgaag aaacgcaaga cttgtggatc aacgtttttg atcatttcat
     3361 gtacagtgat gaaattccct agagatttac tcattttttc atcgtcttcg ccaatcgtta
     3421 catagccatt gtgcatccag taattagcga attttttccc tgttttcgct tcactttgtg
     3481 cgatttcatt ttcatgatga ggaaattcta aatcttgccc gccaccatgg atatcgatcg
     3541 tttctcctaa atgttttgtt gccataacgg aacattcaat atgccaaccg ggacgtcctt
     3601 tgccccatgg tgaatcccaa gaaatctcgc caggttttgc ttgtttccat aaagcgaaat
     3661 ctaatgggtc ttctttttgc tgctgttctt cccctgtacg ctgacttgct ccgacttcca
     3721 gttcatcgat ggattggtca cttaatttcc cataattttc aaatttacgg gtacggtaat
     3781 acacatcacc tgaagattca taagcatagc ctttttcaac caacacttga ataaatgcta
     3841 aaatatctgg catatgatcc atgactcttg gatgcagtgt agcaggctcg acattcaacg
     3901 ctttcgtatc ttcttcaaat gctcggatga aacgatcagc aacttctggt gctgtaatac
     3961 ctaattcgtt tgctgtacgg atgattttat cgtctacatc cgtgaaattc gatacgaaat
     4021 cgactttata gcctcgatat tcaaaatagc gtcgaattgt atcgaaagca atggtgctac
     4081 gagcatttcc gatatggata taattataca cagtcggtcc gcagacatac ataccgacat
     4141 gtcccttttg tatagggatg aattcttctt tttttctggt caatgtattg tagattttta
     4201 tcattctgat ctcctcactt tttctccttt gattcgaacg acttttgctg gtactcctac
     4261 cgctgtagcg tcgtctggaa catctttcaa cacaacggca cctgcaccga ttttggcgtt
     4321 ttttccgatc gttaccggtc ctaatatctg cgcattcgca gagagcatgg ctccttgttt
     4381 aacagttggg tgtcttttac ccgtttcttt tcctgtgccg cctaatgtta cgccatggaa
     4441 taaaatcaca tcgtcctcga tttctgcggt ttcgccaatg acgattccca ttccatggtc
     4501 gatgaacacg ccagttccaa tcgttgctcc tggatggatc tcaacaccag ttaggaaacg
     4561 ccagaattga gcattgatct tcgccaataa aaagagccgg tgctgatata aaaaatgaga
     4621 gaaacgatgc caaaacaagg catgaaaacc agggtatgtc aaaacgatct cagctgtcga
     4681 acgtgcagcc ggatcgtttc tctttgctgc atcaatcgct cgggtaaacc aactcatgac
     4741 tttccactcc tttcgtaaga attttatgta ggaaatcatc ttcttataaa gaaaagaagc
     4801 gccttttaaa taagtttcct tatcaaaaga cgctttccgc gtggttccac ttttcttcat
     4861 aatcaatgat tatctctgac agctaacgcc tgttatacgt tcttggctac tttgtttcct
     4921 ataaaacagt ttttcctgtt ttctgtgttt cgccaagacc actcccagag gcatttcggc
     4981 aaccattcat tgataccttg caccacccgg tatctctctt gtaatgatca gctgcttact
     5041 tttttctgtt catcgtgttt agttcagtac ttgatttaaa tgtgctaatg atttttcacg
     5101 accaagcaat tcgatcgttt caggcaattc tggaccatgc atttgtcctg atacagctac
     5161 acggattggc atgaaaagat tttttccttt tacgccagtt tctttttgaa cagctttgat
     5221 ggcagctttg acagttggag catctactac ttccatttgt tctagctgtg ctttgaatgc
     5281 gtggagaaca gtaggaactg tttcaccagc aagtacttct tttgctgctt cattcaatac
     5341 aggatgttca ttgaaaaata gcgtagacag atcaacgatt tctgcagcat agctcatttg
     5401 aggttgatac aggctaacga tcttcttcac ccattcgatt gtcttttcat ctgggttttc
     5461 tgctacacgt ccatcagcga ttagataagg cagacacatg tcagtaaatg catctagatc
     5521 cattgctttg atgtaatggt tgtttaccca ttgcagtttt ttcgcatcga aagctgcggg
     5581 tgatttactt aggcgttttg gatcgaacat tttgatcaat tcttggtgtt cgaagatttc
     5641 gtcttcgccc acaggtgacc agcccaataa tgcaataaag ttgaacattg catctggtag
     5701 atagcctaat tcgcgatatt gttcgataaa ttgcaagatt gattcgtcac gtttgcttag
     5761 ttttttacct gtttcactgt tgatgatcaa tgtcatatgt ccgaaggttg gtggtgtcca
     5821 gccaaatgct tcatagatca tcagttgttt tggtgtattg gcaatatggt catctccacg
     5881 taaaacgtga gtgattttca tcaaatgatc atcgaccgca acagcaaaat tataagttgg
     5941 cataccatcg cgtttttgta tgacaaagtc gccaccgata ttatcggatt cgaacgaaat
     6001 agcgcctttc accatatctg taaatgcgta ttctgtattg cgaggcacac ggaaacgtac
     6061 aacagattct agaccttgtg cttctttttc tgcttgttct tcaggtgtca agttcgcaca
     6121 tgttcctgcg taatgaggca tttctcctct tgcgcgttgt gcttcccgtt ctgcttctaa
     6181 ttcttcttct gtgcaatagc atttgtatgc tcgattgcta gcaagcagct gatcaatcag
     6241 cggttgatag atttctttac gttcagattg acggtaaggg ccgtattctc caggattttc
     6301 aggagattca tcccaattca ttcccaacca agcaaggttt tccaactggc ttttttcgcc
     6361 atcttcgata ttacgttttt ggtcagtatc ctcaatacga ataataaagt ctccatcatt
     6421 gtggcgggca aacaaataat tgaataatgc agttcttgca tttccaatat gcaggtgccc
     6481 agttggactt ggtgcatagc gtacgcgtac tttcgtcatt ttcttcctct tctttcaaac
     6541 tagactttga ctaacagcaa aaattccgtc agttacggct tctcaaatat catttattta
     6601 aagccactca gcacaaattt taccttttct aaggtaaata gtaaagccaa ctggttgaat
     6661 ttccttacca gttggctgaa tttctttttt attttgattg tttagt
//
LOCUS       DASHRQ010000101         6460 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.107, whole genome shotgun sequence.
ACCESSION   DASHRQ010000101 DASHRQ010000000
VERSION     DASHRQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.107"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(105..560)
                     /locus_tag="U2M88_002624"
     CDS             complement(105..560)
                     /locus_tag="U2M88_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374949.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEIGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(714..1922)
                     /locus_tag="U2M88_002625"
     CDS             complement(714..1922)
                     /locus_tag="U2M88_002625"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEM8374950.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2034..2366)
                     /locus_tag="U2M88_002626"
     CDS             complement(2034..2366)
                     /locus_tag="U2M88_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEM8374951.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2514..3389
                     /locus_tag="U2M88_002627"
     CDS             2514..3389
                     /locus_tag="U2M88_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEM8374952.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3471..4052)
                     /locus_tag="U2M88_002628"
     CDS             complement(3471..4052)
                     /locus_tag="U2M88_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEM8374953.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4058..5317)
                     /locus_tag="U2M88_002629"
     CDS             complement(4058..5317)
                     /locus_tag="U2M88_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEM8374954.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5319..6365)
                     /locus_tag="U2M88_002630"
     CDS             complement(5319..6365)
                     /locus_tag="U2M88_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374955.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1798 a         1336 c         1102 g         2224 t
ORIGIN      
        1 cttgtcacag tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat
       61 aagacacatc tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc
      121 ccgtatacat cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt
      181 tcgctgattt ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat
      241 gggaagtaca caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct
      301 tcgtatttca atgcgcgatt aaacatgcct atttccagtc cgcctaggtt gatatcatgt
      361 gtcgcaactg cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt
      421 tcaacaaatt cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga
      481 tcgccgatat gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc
      541 gctgtcattt ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac
      601 ctcagtttag cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt
      661 atctgcccac tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc
      721 aatttttctt tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct
      781 acgacggctt tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca
      841 gtttcttcta gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa
      901 cgatcatagg attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat
      961 tcttctactt gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct
     1021 ccagtttctc gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta
     1081 gaacacttcc acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg
     1141 atcaataatg cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa
     1201 cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca
     1261 ataaatgcat ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca
     1321 tttatacgtt ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag
     1381 gtttcttcct ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt
     1441 aaggcatctt catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct
     1501 agatcttgca gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca
     1561 ttgatcgtaa aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga
     1621 tctggtctac ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt
     1681 tcatcatttt ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga
     1741 aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc
     1801 tggttcagca agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc
     1861 tctaattttt tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc
     1921 atttttccaa cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga
     1981 ctgtttgaca agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg
     2041 tccttacgct caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa
     2101 acttcagtta agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca
     2161 ccaagttctt ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa
     2221 gtgtggttga cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct
     2281 aatggagaga aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata
     2341 ctgtataaag aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt
     2401 aacgcaaatc ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag
     2461 gaatttctaa atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca
     2521 aacgaaaaac agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg
     2581 gtcttgttta cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct
     2641 tgatcttaag agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc
     2701 cattgatttt actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag
     2761 gtacactttc tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt
     2821 tacagcatga tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg
     2881 ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag
     2941 aacaaaatta cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac
     3001 ttgcttcatt aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg
     3061 ttttcagtcg tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg
     3121 tcgtctccaa caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg
     3181 tgactttcct ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg
     3241 tcgttagctc tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag
     3301 ctttcctttc aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc
     3361 cacagacaag attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag
     3421 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
     3481 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
     3541 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
     3601 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
     3661 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
     3721 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
     3781 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
     3841 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
     3901 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
     3961 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
     4021 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
     4081 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
     4141 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
     4201 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
     4261 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
     4321 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
     4381 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     4441 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     4501 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     4561 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     4621 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     4681 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     4741 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     4801 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     4861 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     4921 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     4981 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     5041 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     5101 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     5161 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     5221 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     5281 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     5341 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     5401 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     5461 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     5521 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     5581 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     5641 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     5701 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     5761 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     5821 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     5881 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     5941 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     6001 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     6061 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     6121 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     6181 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     6241 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     6301 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     6361 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     6421 agataagagc aaagctcacc tcataacttg gaaataaata
//
LOCUS       DASHRQ010000102         6264 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.110, whole genome shotgun sequence.
ACCESSION   DASHRQ010000102 DASHRQ010000000
VERSION     DASHRQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.110"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(95..667)
                     /locus_tag="U2M88_002631"
     CDS             complement(95..667)
                     /locus_tag="U2M88_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEM8374956.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(674..1336)
                     /gene="rpe"
                     /locus_tag="U2M88_002632"
     CDS             complement(674..1336)
                     /gene="rpe"
                     /locus_tag="U2M88_002632"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEM8374957.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(1352..1867)
                     /locus_tag="U2M88_002633"
     CDS             complement(1352..1867)
                     /locus_tag="U2M88_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEM8374958.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(1869..2153)
                     /locus_tag="U2M88_002634"
     CDS             complement(1869..2153)
                     /locus_tag="U2M88_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8374959.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(2187..3515)
                     /locus_tag="U2M88_002635"
     CDS             complement(2187..3515)
                     /locus_tag="U2M88_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEM8374960.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(3529..3984)
                     /locus_tag="U2M88_002636"
     CDS             complement(3529..3984)
                     /locus_tag="U2M88_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM8374961.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(3987..5933)
                     /locus_tag="U2M88_002637"
     CDS             complement(3987..5933)
                     /locus_tag="U2M88_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM8374962.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
BASE COUNT         2059 a         1205 c          875 g         2125 t
ORIGIN      
        1 aattattaag ttatgtctaa tcaaataaaa aaaccattct catatttgct gaataattag
       61 catttagaga atggtttttt atctttaaaa tctactactc aaaatctgca tgtcgagcaa
      121 acatcgtttc tgagctttca cctgttttat ccattaaaga taacactaaa ctatcaaagg
      181 tgagaaatgc taattgttca aaacttgaac ccataggttg tgaaaaagta tcttcttctc
      241 gttgattttc atctttggta atacctggta atacaagaac agaatctgct aattgaccaa
      301 tcgacgagtc ttttttcatg gtaactaaag caatgcttac accactttga tttgctttgg
      361 ccgctaaatt ttttaaacta cctgtttcac ctgagccaga acagataatt aataaatctc
      421 caggttgcga atgtggtgaa ctaatttcac cgacaaggct gactgaaaaa cctaaatgca
      481 ttaagcgatt cgtaaatgct tgcatggcaa ttccagagcg tcctgcacca gctagaaaga
      541 tgtgtttagc ttgaacaatt ttctcactaa attgttctaa ctctacagaa ttaatacgtt
      601 ttgagttatt ttgtaattct aagataatcg cttctaaata gtttttattt tggtctttat
      661 tggtcatgat cttttacttc acttcctcta atgattgatt tagtttatca atttgttctt
      721 gcggggtatt tttcccattg aagatgaaac cgcctgatac aaaaatatcc gcccctgctt
      781 tagaacaaac tttaatcgtt tgattatcaa tcttgccatc tacttcaata tcatacgtat
      841 aatcattttg tttgcgtatt acatctaact gttcgatttt aactactgtc tcttgaataa
      901 aatgcttatt ctctgttcct ggattgattg tcattactaa tacttgatcg actaaagata
      961 atactggttc aataaaactg actggtgttg caggattaaa agtaatccct acttttgctc
     1021 ctgcttcctt gactaactga atgaccttca ttgcgtgttt ggtagcttca atgtggaagg
     1081 taacaatatc tgctcctgct tctacaaatc ttgtgacaat tttttctggt gtatcaatca
     1141 tcaaatgaac atcaaacaca acgtctgtta gtggacgaat atctttaata acacttggtc
     1201 caaaagccat atctggtaca aaacagccat ccattacatc aatatgcaaa taatcaacat
     1261 tcttttcttt taagatttca aataactctt taacttctgc gataggtgca tttaacaatg
     1321 atggagcaat tgtcattctc tattcccccg tttattctcc cataatcatg agattgattg
     1381 ttctagttcg ctctcttagt tgatcccaat cgacaaaata gacacggccc atttctccta
     1441 ataaaagttt accttcttga acaattaaag tcattgagtt ggttccataa atagatgaac
     1501 gtaaatgtgc atcggtattt agcatgaccc atttttctgc agggtaattg ggatcagata
     1561 atcttgttcc aaaatcaatg tgttttggcc ccggactgtt gtattgattt tcactcgtca
     1621 tttttggaat aatcgtttcc attacattgt tgatgtctac atgtaaataa tcatcaccaa
     1681 aaaagtttgt gtcatgcatc gtttcttcaa aaaagagtga gcaagttgta tgcgggctcg
     1741 aaattactaa cattccattc tcaatacctg ctgtatcaac tgtatctaaa agttcttttc
     1801 gaatatcatg atacgaagga cgccctgaaa ctgtttcaat agtcaaagtg tcatgatgaa
     1861 aacgcatatt aaacacctaa tctttctttt acatctgcat aaatggcatc tgtaccaata
     1921 cctgttaata agcccaagcc attcgttact ttagctttaa ttacatcagg gacaattgtt
     1981 gtacttacta cagcatcata ttcatcaatt ttatttagct catcagcaat tttagattga
     2041 taaattttca cttgatctgt taatccttct tcttccaacc attttgataa ttttgctact
     2101 gcaattgtag aagtagcatg acctgctcca caaataacta ataatttttt catattgatc
     2161 tcttctttct tattttaatt ttatgattaa gctttcttca ctttgttcat gtagatagaa
     2221 gcacctacta aaacaactcc taatccacct attccaatta caccaaagta agatgctaat
     2281 aaaacaaaca accctactgg ccataatcca cataacaaca tagttactaa tcctgctgtc
     2341 cccacatcga aacttgctaa tgtaaacaca tcagtgataa ttggtgccaa ccatgttgcc
     2401 atatataatg ttgtaacaac atacaacgaa catcctacta atgatctcac aatatttcct
     2461 ttaaaataag gaatcattaa acagatggcg aaagcaaaga atgctaaatc tccaaaaggt
     2521 aatactttat ttcctggaag aattagtgct agcaataatg agattggtac aagcaataga
     2581 gaagttgaca taatagttgg atgacccaca gttaacgctg catccattcc aatgtttact
     2641 tggcgaccat ttaacttttt attcgtaaat tctttcgccg cttctgcaat tggcattaaa
     2701 ccttccataa acattgctac cattttaggc ataatcttca tcactgccgc agtcataatt
     2761 cctaagtttc caattcctgc tacgtcatag cctgccataa tacccactac aataccgata
     2821 attaggccaa taacaactgt gtcacctaaa acaccgaaac gttttgttaa tgtttctgca
     2881 tctgcatcta ttttattaaa tccaggaata cgatcaaaaa cccaatttac tggtttggca
     2941 aatagtactc ctgataatcc catcatatgt gtgatagaga ttcctggata gccgaaaaat
     3001 tcgctaacag ttttttgtgt acgatctgct aagaaccatt gtaaaattac tgcgacaatc
     3061 gcgccaatta agccaaacca aaagtttccc gtaacagatt gagtaatcat acctaggaaa
     3121 attgcgaacc aaaagttcca aatatcaaca ttcaatgttt ttgtccaacc caaaaccaca
     3181 aatatgaagt taagaattaa tgaaattgga atcattaaaa ttcccaatga tgtagagtaa
     3241 gccaaaggtc caccaacacc tacgccaata tctaggatat tcaggccacc accgtattgt
     3301 tcccccattt gttgaattgc tgttcctaaa ccatctgata ataaacttaa aactaatcct
     3361 aaaccaatag aaccgatacc aacagtaacg ccggaaacaa atgctttacc aggttttaat
     3421 ccaaataata tcccgattac aaaaataatc agtggtaaaa agacgtttga ccctaatcca
     3481 ataaaccatt caaaggcatt taatacaata tccatctctc tattcctcct aataaactcc
     3541 tgcagaatgt actaactcaa taaatgcttc tttcgtggta catgcaagta attcatttac
     3601 tttttcttca ttttgaaata ggctaactaa gttttgtagt gtatcaattt gttcatgagg
     3661 ttgtgacatt gccaacataa agactaaacg tacaggtatt tcatgagatt tatctgtcat
     3721 atctgaaaat accagtggtt cctttaatga ggcaaaacag atttgagagc tatttacatg
     3781 tgcggaatct gtatgtggga tagcaaaccc aatttgattt acttctaatc cagttggaaa
     3841 ttcttgttca cggttggtga taccttctaa gaaactatct ttcacgcaac cagcgttttt
     3901 taattgttca gacattactt gaaacaattc tttttgatct ttcacttcta attgaaataa
     3961 acataattct ttatctagat acatttttat tctccttttt gttgaaaaat tcttagtaat
     4021 ttttcgctat catgaacttg taacaagcga tcgaccattt ctttttgccc aataaattgg
     4081 taaagttctt catatactcc tctaatctca gatacatctt cttcagctaa actaaccata
     4141 atgactaatt tgacttttcg atcgccccaa ttaatcgcat tttttaatgc tacaataaaa
     4201 atgttggatt caatgaccat tgatggattc gcatgcggta aagcaacacc actttctaaa
     4261 ttagtattcc ccattttctc tctagagaag atagaagcac gaaactcttc agtaactaat
     4321 gaattggctt ctagttcgga aatcatcaaa tccaaacatt cttctttgct ttccacctgt
     4381 actcctgttt ttactaaaga tgattgaaca aatttagata tggtgggaac tttaaccgct
     4441 ttaggaatca gttcattttg aataaatcgc gaaggttctt ctattaaata tttagtataa
     4501 gcttccataa tattcatata atctgtttta cttaccaatg gagataccct tactaagggt
     4561 ataccacctg catcaaagga aagagaggaa ataatcaaat caacttcccc aatttcagct
     4621 aaatgaaact tatctaaagt tgatatttct acattatctt ttgctggtaa aaaccgtcga
     4681 actttattgt aaattaattg cgcagaagaa ataccgtatt ggcaaataat gatgatatta
     4741 tttgctttgg actgatcttc aatagcaatc tgaaaatgaa tcaaaattaa ggaaacttca
     4801 tcgtccgtta aagtcacatc aaattcttct tcaaggatgg acatggcgta ccatactatg
     4861 ctaaagagtt ctgaatattg agatttgata ttttctaata acggattttg aattttaata
     4921 ccttttttta aacgaaggac cattgctggc acatgataca ataaggattg atataatcga
     4981 tcattttgag tcaaatcaat tttttcaatc tcactcattc tttcaatcaa tttatgaacg
     5041 ctcatagcgt actcttcatt gctggttttt aaattatttg tcacacgatg tgcaaatagt
     5101 tgacgacata gatattcttt atctgcgtca taaaatggaa gattcaattt tgccttcaat
     5161 tcttcaatta aaccatttgc cacaatgtac gtttccatat agcgaatgct attgaagagg
     5221 aattcttctt caacagttaa atgatgacct ttcagtaaac ggttacattg gataactaaa
     5281 gtagaaatca atgaccgaac atagtaatca gaaacctttt cagtcagttc actgtaatca
     5341 ttgaatagca aatcatgaat gacactaata acttttgaat caaacaacat atttaaaact
     5401 tgaaaaaatg tttttgattc catgtcattc gcataataga atacaagttg cttgattgct
     5461 cgttgaattg cttgttcatt accagtaaaa ctaattcctt ctgcagatga ctgcaactta
     5521 acttctccac tgccaataat tttattgata attctcaggt cattgtataa cgatgtttta
     5581 ctaaccataa aactttgaga caaatgagtt aaagaaacag gcttatcacc aaacaaaact
     5641 tctttaatga tctcgacacg tcttttttct ggagaataaa tatctttttc aagataattt
     5701 tctaccgatg aaatcaaatg ttcaacgttt ttcctatcac ctatgagttt gatacccaaa
     5761 gaaggtaatc gctcaattgt tacttggtaa ttaactacat aatcttccaa caattcaata
     5821 tcttgataaa tggtcttggt cgacactgcc aatttatcac taaaaaaaga agcgggttta
     5881 aagtcttctt gtcgacttaa taattttaat aaatttacat gtctttcaat caaacttgtt
     5941 cacctcttaa cgctagcatt tgttacctct tttcattatt tattctagca ctggaacccc
     6001 cttccctcaa ttacatctat gtcaataata cttgtggaag tttgttgtaa aggaaatcca
     6061 aataatctta aaaaatgaga aacaaaaaag gagaaatcct taaaagagct aagatattcc
     6121 aatagaaaat actccttatt atggaagtaa tatgaaatat tacaattgaa aaataaaaac
     6181 aatataatgg cgtcactaaa attcgtattt ttttattata atataactta attacaaatg
     6241 ctcatctatg aacaattgag tcct
//
LOCUS       DASHRQ010000103         6255 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.111, whole genome shotgun sequence.
ACCESSION   DASHRQ010000103 DASHRQ010000000
VERSION     DASHRQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.111"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            54..506
                     /locus_tag="U2M88_002638"
     CDS             54..506
                     /locus_tag="U2M88_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374963.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            520..1209
                     /locus_tag="U2M88_002639"
     CDS             520..1209
                     /locus_tag="U2M88_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374964.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1334..3685
                     /locus_tag="U2M88_002640"
     CDS             1334..3685
                     /locus_tag="U2M88_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEM8374965.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3765..4160
                     /locus_tag="U2M88_002641"
     CDS             3765..4160
                     /locus_tag="U2M88_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8374966.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4485..5102
                     /locus_tag="U2M88_002642"
     CDS             4485..5102
                     /locus_tag="U2M88_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM8374967.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5143..5442
                     /locus_tag="U2M88_002643"
     CDS             5143..5442
                     /locus_tag="U2M88_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEM8374968.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5476..5643
                     /locus_tag="U2M88_002644"
     CDS             5476..5643
                     /locus_tag="U2M88_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEM8374969.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5688..6251)
                     /locus_tag="U2M88_002645"
     CDS             complement(5688..6251)
                     /locus_tag="U2M88_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM8374970.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2150 a          864 c         1252 g         1989 t
ORIGIN      
        1 tatcttatcg tagatatata ttataaattt aataagaaag cgaggtgaga atcgtgaaaa
       61 aaacgatact aagtattttt tcagtattta caatattaat tgggggctat tttcttttga
      121 atttagattc tgttaattca aacgctcaaa taaaagatca aagagtagct gaacataata
      181 ataaaaaaat gagtttgacc aaaaatatct attctatgtc agatgaagaa ttgtataaag
      241 aatttggtga tttatccggg gaacagttag aagaaagtat tccaggaatt agtgactcag
      301 ataaaattgt tgtcttgtct gctggtgggc aattaatggg agatggcaaa tccgaattag
      361 ttagttataa acaaaatcac agacagttta ttgatgttaa tactgattcc aacgctattt
      421 cgattaagga tctaaaagag cttttaaata gtaaaagaaa tgaattaaag cagataaaag
      481 aaactaattc agtggaatta gagtaaagga agattcatta tgaaaaagat ttttggtgtt
      541 tctgttgggc tacttatgat gttaacattt ggaaatgcag tatatgctgc tgagaaagaa
      601 gttatttcag atccagagca gtacgcctca cgatactttg ctgattttga agaagaagat
      661 aaagtacttg cattgccaga aggtggtttt ttaaaagggg aagctaaaat tgtatctatg
      721 gataatccag atataattca aggttcatat aattcagaaa ctgatcctgc ttcagtttca
      781 gtgacagaag caaaaaaaat tgttgctgaa aaattcaaaa atgatattga aactactcca
      841 attatgcctc gaggagcatt tccacccgat gttggtacta gcggacacct tcttgtacta
      901 gcaggaggag gaagctataa atcacagcca tttagtggtg cgggatggcg ttttggaggt
      961 atgggtgtaa aaccagctga tggtacaggc aactttttac gttggaaatc ctacattgac
     1021 gatggaagag taggaaatgt aaatcaagcc aacgcaacta aatctggttc tattcaagga
     1081 atggcattgt atgctcgtga aggattcaaa tactttgcac caaacgggcc tggttggaca
     1141 atgaatatag gtatttatta cacttataat cctgttgcag gtacgtatta tgttgttcaa
     1201 aatatttaat aaaacagcac aaagagattt ctttgtgctg ttttattatt tttagttgtt
     1261 gacaaacact aaaatataaa atataatcaa cacataaacg aacgtatgtt cggcaagaaa
     1321 gcaggggatt tttatggatg agatggtttt aagtactcag aaatggctaa acaagaaata
     1381 cagtaatgtt acagggtttg ataaggttcc agaaaatggg cgaacagggt ggcctacaat
     1441 ctatggactt attgaaggac tacaagtaga acttggtatt actaatctag tagctaattt
     1501 tggacctacc actgaaaaaa tgtacgataa tcaggttact cctaaatggg gcaaaaatct
     1561 acctaaaaat attgtatttc ttattcaagg tgccttttgg tgtaagggga ttaatccagg
     1621 tggcttcgat ggggtatata ctccatattt agatactgct gtaaaagagt tgcagactga
     1681 tgcagggttt aatggaagcg ctgtaactgg agttttggat gcgaaatggg ccaaagcgtt
     1741 atttgatatg agtgcttttg ttcttgttcc cggcggagat agtaaaatta gaaaaatgca
     1801 gcaatggtta aatgtaaatt atttagaata cacgggaata atgccttgtg atggtattta
     1861 tcaaagaaat tcaaatcaag cattaatttt tgcattacaa gctgaactag gatattctcc
     1921 cagtgaagca acagggtctt atggtaatgg gacaactagt aaaacttatc ctgtgtctga
     1981 aggtaactcg ggaaactatg taagaataat tcaatatgga ttatatgtta atggttattt
     2041 tgaagacggt acattcgatg gtattttcac taaatatatg ggtttagaag tactggcatt
     2101 ccgaaaattt atggtattgc ctgaatatac tgaaatagca gatgttgttg taataaaagg
     2161 actattgtct agcgctggag atattagaag atctgcagat ggcgcagata cttctacgca
     2221 attaactaga tctcaaatcc aaacattggt tgataatgat ataaaaatag ttggtagata
     2281 tcttactggt actgttggaa ttggaaaaga tgaacgaaat aagtatctaa ctacagagga
     2341 attgaataat attttttcta aaaatttatc tgtgtttcct atctatcaag atggaggtgc
     2401 agcattagct tattttactt atgatcgtgg cttatcagat ggtaagaagg ctatagatgc
     2461 agcaaaaaat ctaaatatac cattaacaac agttatttat tttgctgttg atctagacat
     2521 gttaggggaa gaaattcttg catatgctgg agaatatttt aagggggtat ctgctgtaat
     2581 gtcatatagc gggtatcaaa cgggtgttta cggaacaaga aatgtttgtt ctcaaataat
     2641 caataacggg tatgcttcgt ttgcatttgt ttcagatatg tcgactggtt atagtgggaa
     2701 tttaggtttt cctatgccta gacaatggtc ttttgatcaa tttgttgaat ttaccatagg
     2761 aagtggcaac ggagctgttg gtatcgattt aatcgctaca tcgggtagag atagcggatt
     2821 taatgagtta tcaaatgata ctaataatga taattacatt gccaaataca atcagaaggt
     2881 tataaatcag atggtacgtg cgtatcaata tctatcacaa gctggtcttg ataatccttg
     2941 gaaccctcat ttgacatttt atagatatgt aaattattct ggtttgtcat gggacatcat
     3001 ttcttctcct gtaacagagc atgatagaaa aatttatgat gaatatagga ataaattaac
     3061 taatagcgag ggcctgtata attattttat agatcctaat actggttcaa taataggttt
     3121 gcctcactta attgtgacgt tacaatcaca gatgtttcta gccgacacaa tcaatgatag
     3181 tgtttctgac tttgcaggtt ggttaggaga tttaatgact tgctggggtg aagttaagaa
     3241 attaggaata caaatggagc aaggtgtatt tgcattagta ggtacggctg caggtggaac
     3301 atttagtcta gaagatttat accaagatgt tgacgcggta aatgtttata ccttagcaaa
     3361 aagaaattct gattcaacag ctttatcggc tttaaaaacg tactattgtg ataagggtgc
     3421 tattgataga tttaatcaat ttgttaagaa cagatttggt tcttcagata aaatcgcttc
     3481 agtaacaaat agtttattag aaaatgacga aacagttgat atcttaggtg tagggataat
     3541 ttcatttttc attgataaaa tagttaataa ttcacttggg acagatttta cttatcttga
     3601 ttttattgga gatgagggga aagttgcaaa tggattttct gaaaaaatta ttcaatttca
     3661 agaaaataga agtaaactta attaagaatc gtttactatt taaacagtgt ccttttcctt
     3721 tttaaatata tacgataaaa taagtatatg aattgtaaaa ggacatgatg attatggaag
     3781 aaatagaatg gaataatctg actcaaaata ataaaaataa ttctggatgt ggaattatct
     3841 ttatgtttgt tatagttttg ggaatagtag tacttttaat aactgtcaag atagtagatt
     3901 actttgcgaa tactcctgaa gaacaaatac agagaaaact tcctgagttt gaaagagtac
     3961 caccttatga tccaagagat aattcatttc aagtaaaaaa attaaaagag tctccaaatg
     4021 aaatgtggtt tgaagttaca gttgaatcaa gcgacttcaa agaaaaaaag aaagaataca
     4081 attatattgt aaaggcaaaa gtatcaaaaa gatttatttt taaccctaaa ttagaaatat
     4141 atagtgttgt aatagaataa tagtgttaaa aaaattgtaa aagttaaaga agtcaaattc
     4201 tgatgtgaaa aataaaatat accatggaag acataaaaga aaaactggat aactagttta
     4261 tttaatggca gaccgttaag gcctgccatt tttatgtatt ttagcggaat aaatataatc
     4321 atgcgggagt gtaaacttac tgtttcaaaa agtatacttt ttgacatgcc ttgattttct
     4381 ttgtttatgt atggcataat aaccacataa tacaatttga aacgtttctg atttaatctg
     4441 attgttttga aacaatttta gtgaaaaaag ggtagataaa aacgatgaga aaattttttt
     4501 atatccgggt cagtagttat gatcaatcga tcgaacgaca actgatttcc gcgaaggaat
     4561 taggaatccc tgaagaatat atatttgtag aaaaagccag cggcaaagat tttaaacgcc
     4621 ctgaatatca attaatgaaa cggatgtttc gaaaaggaga tttactctat ctacaatcat
     4681 tagatagact aggcagaaat aaacaaatga ttttagaaga atggcgagaa ttaactaagg
     4741 aaaagcagat tgatatttct gtgttagaca tgcctctgtt agatactgct agttacaagc
     4801 aactagatgg gttagagacg cttatctctg acttaattct gcaattactc agctatatgg
     4861 ccgaagacga gcggaaacga attaatagcc ggcaaagaga aggcgtagaa gctgcgaaac
     4921 aaaagggtgt aaaattcggc agaaagaaaa ttgctttacc tcctgaattt cctgcgatct
     4981 attctgactg gaaagcagga aagataactg cagtctatgc tatggatcaa ttgggcatga
     5041 aacgaaatac cttttatcga cgggtgaaag aatatgaaca taccttgcac cacttttctt
     5101 gatagatata acagctatta gacgggaagg gagagtttga aaatggactt aacgaatcaa
     5161 agggtcgttt ttactggaac gctgcagcaa atgacacgaa cccaagccat cggtttggtt
     5221 cactctttaa acggccattg tcaatcaagt gttacttcaa aaacaaattt cctggtttgt
     5281 ggtcagtatt ctaagtcgtt gtttgatgat tctctctata caaaaaagga acaaaccgcg
     5341 cgggacttga ttgcgttagg tcatgagatt accattttat cagaagtcga attttatgcg
     5401 ttaattagtc aacagttgaa agaatggcaa cggtatttat aatcgttagt aaaatttaca
     5461 aggaggaaaa tttttatgaa gcgaaaatct tatgatcgtt ggggcattga aataactagt
     5521 tttaatcgaa atattgcttt tcacgtggag tgtgactgtg gggaattagc aaaaagtgtt
     5581 tatcaaaaaa attactttta ctgtcctaaa tgccatcgcc gatacggggt acatggaaca
     5641 tagaaacgaa atagtgcggt agaaaagcga atgaatgtag tatatttcta caaatcattc
     5701 gctttcttta ttttataaat agttggtcga gagatcccgt tttcttttgc aatctctgca
     5761 actggaatgc cttgctttaa catgtccacc acggttttaa aaactaagcg tttttgcgga
     5821 tctgttgagt taatagaata ctcgattggt cgtcccttat atcggccggc tttttgagcc
     5881 aataatactc cttgccgctg acgttcacga attttctcac gctcgttttg tgcaatataa
     5941 cttagcagtg ataaaaacat atcgaacata gcggtatcca ggagttcatt tccagtatta
     6001 aaatctaaaa actgtgcatc taatattttt aacgctactt ttttctgttt cataaaagca
     6061 actgtatttt ttatatcttc ataatctctt cctaaacgat ctagtgatgt aacgatgacg
     6121 caatcacctt cacggatata atttagtaat ttttgaaact caggacgctc agtatcttta
     6181 ccactggctt tttcttggaa taatttatca atgttaagtc cttcggttct gttgcgaagt
     6241 ttgaaaatca aacgc
//
LOCUS       DASHRQ010000104         6208 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.112, whole genome shotgun sequence.
ACCESSION   DASHRQ010000104 DASHRQ010000000
VERSION     DASHRQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.112"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(63..1268)
                     /locus_tag="U2M88_002646"
     CDS             complement(63..1268)
                     /locus_tag="U2M88_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEM8374971.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(1291..1444)
                     /locus_tag="U2M88_002647"
                     /pseudo
     CDS             complement(1291..1444)
                     /locus_tag="U2M88_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(1447..2832)
                     /locus_tag="U2M88_002648"
     CDS             complement(1447..2832)
                     /locus_tag="U2M88_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEM8374972.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2861..3247)
                     /locus_tag="U2M88_002649"
     CDS             complement(2861..3247)
                     /locus_tag="U2M88_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEM8374973.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3263..3577)
                     /locus_tag="U2M88_002650"
     CDS             complement(3263..3577)
                     /locus_tag="U2M88_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEM8374974.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="U2M88_002651"
     CDS             complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="U2M88_002651"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEM8374975.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4837..6126
                     /locus_tag="U2M88_002652"
     CDS             4837..6126
                     /locus_tag="U2M88_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEM8374976.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
BASE COUNT         1799 a         1257 c         1171 g         1981 t
ORIGIN      
        1 aggttttgtc caaaattcat aaaaatgttc tcctctctat attcaatgaa tttgtatttg
       61 agttattttt ttgttgttat cacgtcctgt tcttttactg actgttgctt caaaatctgc
      121 ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt ttacaacctg cgtctggttg
      181 atttcatcaa gtttaatcgc aacctttaag gtcggggcaa cttgatgaga tagccagttc
      241 agcgtccttt ggaaggagta aggctctggt tttgtggtta gttttaatcg ttcacgattg
      301 ttcccaataa accaagccca ttcttcattc agtttccaat cagaacgagg tttggaatcg
      361 tctttatcta caaaacggat ataccgattg ataattttaa aggcggtatg ctctggattg
      421 tcatagacga gtaaatcacg gactgcataa taggcacgct catttttcaa tcgaatctca
      481 aaacggtttt ttacttctgc gtcttcaatg ggaatatcat ttttcttgta ctgctcgtag
      541 tccttttcat agatacagaa ataaacttca ctttgtaatg aaccgatata gagggtgttt
      601 cccatacatt ccttttcctc tttgcgtacc agttcgccac tgcgatagct tttaaaactg
      661 cggaagacgg agatacattc ttcctgttgg cacttttcag tgagtacagg gatatttaaa
      721 atccctgtct tatcgttaat ggcaaggtca aggcgtttca tcacaccgcc agccaccaaa
      781 acgtccataa agaactcata ccagcttctt tgttgtgcca gaagatagct ttcaaattgt
      841 ctgcacccac gacctttcaa ttccaccaga actcctttgt ccagttcatg ggagcaaagg
      901 acgaatatgt cgcctaaagc ataatgctct gaataagaat agaaaccata gtcctcatga
      961 agaaaatagg acagtttcag ttgtaagatg ttttcgacca cctgctgtac gtctgttgtc
     1021 ggaaagcgaa ttcttacata atcaaacagc atttcaaggg gagcgtcggg attgaagcgt
     1081 tccagagctt cccaaaggga ctgctgtaaa tcctctgatg gcttgacttt tcctgtttca
     1141 atatcgctta gatactgcct tgtaatacca gtcgcaacag ctaaacggtt ttgagatagt
     1201 ccataagcca agcgtttttc ttttaaatgc tgtaaccaag tttgttcatt cagtaaaaat
     1261 ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca tttgacatac gctgaaattt
     1321 tgtaaatccc ttgtaaccaa aggattttct aatgtttttt tgactgtttc ctgtcgattt
     1381 gtacccccct gttagatacc gaggggttaa gtgctggcgt ggctattgcc acaccagcca
     1441 gcaagatcag tccacacctg cgacttccgc ttcgcacgtc gcctgcgtgg actgtctgct
     1501 gttggataac tttttaattt cctccaagaa atcatatcct tttggtacaa ggggagtata
     1561 aaactctgat atgacacttg ttcctacatc aacatagcca cgacctttga ttcgctttaa
     1621 gaagaaatcc ttttgtacgt cactgccaaa catcatgcca tagcccattt cagacatacg
     1681 acctaaagcc actctgaaat taaactgatc acggattccg tcgcctaaat attttgcgtc
     1741 tggacgttga caagccagta ttagaaagaa gccagcttga cgacctaaca tgacaatctg
     1801 tttcagctta ttcataactg cggtgttttc ttttgttccc agcatttcca tgaaagcgac
     1861 gtattcatca aagattaaga agtgtgccgg gagacctaag taagcataat ttttgccagt
     1921 cttatagttc ttcatctgct tcatttcctc actacgtttc atcatttctt catagaatgt
     1981 ttcaatgcaa gaaagcaagt cttcttttct atagtagaca tttgccatca cagaacctaa
     2041 gtccgcaagg tcagcatttt tcgggtcaag aatatacagt tttgaatctg tatgaagcaa
     2101 ggcttcaatc agtgtcagta taaagtaagt tttaccgcca cctgtaccac cagcaatcaa
     2161 catatgaggg agcttatcat attcccacca tacgtttttc attaagcgaa gtttaccatc
     2221 tttagcttct acttcatcaa tagaaatacg actggctatg gtgtcataga gcaaagtata
     2281 ttccacatag gaatccttta actctttatc cgtcagctca cagtacaagc cactctctaa
     2341 tttcttttcc aagtgtaaga gttggtcttg atattttccc agcgtgattt ccacccgtat
     2401 ctgtatcaag ccatttttaa gtcgataata cattttaggg aagtaggtta tcttttcctt
     2461 tgtacgacca gcactatctt taaagaaacc ctctgttttg acctgttcag attcatacca
     2521 cttgttttca agtatcatct ttgccagttt ttgacggtgg taaagttgtt taaccgtatc
     2581 atagcgaacc cgtttgaata caaacgctac cagcaagcag ataagaattg cgacactgaa
     2641 actgataatt aaatagggaa tgtcaatctt atctgcttgt gataggttaa aatcctgcca
     2701 gttgatctgc tggattgtct tcacatgaaa cagtccgaca accagcagga aaacaggcag
     2761 gagtgacgct atcgtaaaat gaaagactaa atctttacca gatgggcgaa tccttttacc
     2821 acgctgtttc atgcgaaaaa gtctcctttc tacctagcga ctatttgtct tgtgtcggtt
     2881 ctttctttgc ttgtggttga gctttgaatg aactagaatc ctttgtcagc acaatatcgt
     2941 ctgccttgat ataccagtca acatctgctc cttgataggt ggcagtagca acggtgtccg
     3001 caatgggatt gataagttcc acccgtgcgt tataatcaaa ctctttcaaa ggcacgctgg
     3061 caggaatact tacttgaatc atgcgtcctt gtcctttgga ttttaagtca taggtacgtt
     3121 ccttgatttc atctgaaacc gacccgtctt cattttggat tctcacttca cgacgtagag
     3181 cagagaattt caattctcca aaagtcgtgt ctttatctaa tacaatgcca tttgctaatc
     3241 tcatcatttt tcctctcttt ctttattctt ttatcatgtc gtcagcatgt aaaaggtaat
     3301 ttgtaaaacc acgagtgccg attttgtagc cctctgcggt aatacgtgga ttgactaact
     3361 tcacacgttc ctcaaagccg aaatgttttt cgccagcttc agcaggaagc accaccacaa
     3421 tatcatctgc tctttgaaca tcagaataga gattatagct tcttgataag acagttagcc
     3481 gtccgttgat tcttcgctga acgactttat cctcgccagc aaattctaaa ttgccgaatg
     3541 ttttttccat gttgggaatc acaaatttaa gttccatatt tttacctatc ctttcttttt
     3601 tattggctga atgaatgttt gatggtctta aagagtgggg aacgaccttt tgattcttga
     3661 ttttttgttt tcataagttc acttcctttc aaaatcgggt aaaaaaatag acacctcatt
     3721 ttttgaagtg tctacctatt aaatattcaa attttattgg aagtatcttt atatcttcac
     3781 ttttcaagga taaatcgtcg tatcaaagct cattcataag tagtaaatta gtagtaaatt
     3841 gagtggtttt gaccttgata aagtgtgata agtccagttt ttatgcggat aactagattt
     3901 ttatgctatt tttctcttaa aaaatatcat tatttgtggt atggagcccc cgcgttgatc
     3961 cggaaggcac ggtagatttg ctcgacgagg atcaggcgca tcaattgatg tggatacgtc
     4021 attttgccaa aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa
     4081 gaaccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt
     4141 ttagcaaaag attcgcttgt gggatttttc ccttcaattg ctaagccata gacgtattct
     4201 tgttctttga ttttcgctaa gatccgttct ccttcttttt cttttacttg tctcatttgc
     4261 gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc gattttcgca
     4321 taggcattca gccgtttgac atactcattg ataccttgga tcaagtattt ttcttttaat
     4381 tttcctactg aaataatttt gatattcatg ttttctctcc tttgatcttc acgctcactt
     4441 tgtttattat aatcaatttt ttcgtctata tgtttatttt gactattttt gttaaaaaga
     4501 tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag cgttgtttta
     4561 ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata
     4621 cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac
     4681 aatatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag
     4741 tttttcataa aaagttcaaa gtctcaaagt agactaaagt caacaaataa gactatacta
     4801 acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca
     4861 aagatgaaaa aaaataaaaa taatagcctc tggcgtaagt tgggtctggg gcttgttgga
     4921 gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg cactggcaac
     4981 aatagttcta ctgctacaag cggcaatcaa aattctgccg gtgaaacagt tgtggaaaat
     5041 gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc
     5101 gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg attattcggg
     5161 cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc
     5221 ggcgtaatct acaaaaaaga cgggaatact gcttatgtag tcacaaacaa tcacgtagta
     5281 gatggacagc aaggtttgga agtcatgatg aaagacggta cgaaggtaaa agctgaatta
     5341 gttggaacag atgcctattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa
     5401 acggttgcct cttttgggga ctcaagtgct ttgaaggttg gtgaacctgc gattgcgatt
     5461 ggttccccat taggatctga atatgcgaat tctgtcactt caggaatcat ttcttccttg
     5521 aaccgacaag taacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa
     5581 acagatgcag cgatcaaccc gggaaattct ggtggtcctt tagtgaatat cgaaggacaa
     5641 gttatcggta tcaactcaag taagatcgca agtacttctg aatcgacttc taatgtaagc
     5701 gtagaaggaa tgggatttgc catcccaagt aacgatgtag tgaatatcat caaccaactg
     5761 gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc
     5821 gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc
     5881 atcgtgacaa atgtccaaac agctacaccg gcagaaaaag ctgggttgaa acaatacgac
     5941 gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg
     6001 tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa
     6061 acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa
     6121 aattaataaa aaaggctctt tgtcaataag gactgatgag ttgtgcaaaa tcaaatctga
     6181 gtcagaatga accacattct ggctcaga
//
LOCUS       DASHRQ010000105         6056 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.113, whole genome shotgun sequence.
ACCESSION   DASHRQ010000105 DASHRQ010000000
VERSION     DASHRQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.113"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..134
                     /locus_tag="U2M88_002653"
     CDS             <1..134
                     /locus_tag="U2M88_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM8374977.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(220..1314)
                     /locus_tag="U2M88_002654"
     CDS             complement(220..1314)
                     /locus_tag="U2M88_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEM8374978.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1503..2264
                     /locus_tag="U2M88_002655"
     CDS             1503..2264
                     /locus_tag="U2M88_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEM8374979.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2379..2819)
                     /gene="fabZ"
                     /locus_tag="U2M88_002656"
     CDS             complement(2379..2819)
                     /gene="fabZ"
                     /locus_tag="U2M88_002656"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEM8374980.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3039..3794
                     /gene="fabI"
                     /locus_tag="U2M88_002657"
     CDS             3039..3794
                     /gene="fabI"
                     /locus_tag="U2M88_002657"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEM8374981.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3904..4917)
                     /locus_tag="U2M88_002658"
     CDS             complement(3904..4917)
                     /locus_tag="U2M88_002658"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEM8374982.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            5082..6002
                     /locus_tag="U2M88_002659"
     CDS             5082..6002
                     /locus_tag="U2M88_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEM8374983.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1860 a         1202 c         1079 g         1915 t
ORIGIN      
        1 aagtgaatca gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat
       61 cattgaatta cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt
      121 ctagcttaat ttgacaaatc ataacctata aaaaaacaaa ataaaaaagc agaaataact
      181 ggctatttct agcggattat ttctgcaata actaattatc tattttttat tttcaattaa
      241 tttaaagctt gaccatggtt tcaaaaagtc aaggaggaat aattcgtcat cggagattct
      301 tcctacaaca ttgacacgtc cgtcgttttt catatccttc aaggcgattt gtgtttctcc
      361 tttgtattga ccgtaccctt cattgtcaat cagtacatcc ccatgatgca tatctcgcgt
      421 attatgaggc gggaaatctt tatctttgta gtatacacga gtcatcgttg aacgaaggat
      481 atattctgag cgatcccctc gataactatg aaggttatca aacaagcaga ttcgttcgtt
      541 ttctgtaata ccttcttccg tatctacttt caatgttgga taatccgcgt taaatgcttc
      601 tgccatttct ttcaattctt cttcacttgc gtacgcattt ccaatagaaa tatcatcaat
      661 caatcctgta aggattaaat gcttcacttg tgtcgcaatt tcaagatctc ggtggtcttc
      721 cagcgtgcac aagccatctt gcgtaggcca agggccaaac tcagcagatt gcgaattgac
      781 gaatgccatc gtattcaaat tatattttct gaatttttct gaacaaaaaa caaagtgttc
      841 gtatcctaaa ccagagtatc gatgaggata aaaattatga gaacctaata aattatccgt
      901 gtttggcgaa taactcatga tgttgtcaac gtaattcgtg cccgaactca tattgatctc
      961 gattttgata ccaaaaggat tgcgcgtcat ttttgcttct tctgctcctg tgaatccgat
     1021 atctaagcgg accccgtagg cacccatctt atggaaaaaa gataagtcat cgtaagaaat
     1081 ctccaactgt tcaaataacg ctggattgat atcaaccatt acttccatgc ctaggctatt
     1141 cgcgtaatca actacttctt taaattctga aagaactttt tctttatcat cattgatctg
     1201 cagcaaactg gtaaaaaccc gtttataccc atacttatga gctaaatcca agtacgcttt
     1261 gtccttttca aatgttgacc gttctggata aatcgagatt cctaattttc ccatattacc
     1321 atttcctcct gcatcttttc gtttttgtat ccacttacat tatagcaaac acaaagtaaa
     1381 tggtctacac cttttctttt tcttttaaaa taaatgataa attccaataa tttaagtagg
     1441 tattttccct ctattttgtt atcattaaag ataacaaaat agtaaattgg aaaggaacag
     1501 ttatgaagaa aaataaagtt attttaagta ttagtttagc agcagcattg ctgtttgctg
     1561 ctggttgttc tgcaaacaac acgacttctt ctagttcgtc tacagaaagt tcaaaaagta
     1621 catctactgc ggaaagcacg actgccagta gcgtcgattt gaacgcattg gaattaccac
     1681 aattgacgaa agatgtcgcc gaaaatgaag atctggtcga aatggtgacg aataaaggca
     1741 caatcaaaat caaattattt cccgaacacg caccaaaagc ggttgaaaac tttatgacac
     1801 atgcaaaaga cggttactac aacggactga ctttccatcg cgtcatcaaa gatttcatga
     1861 tccaaggtgg cgatcctaaa ggcgatggca ctggagggga aagcatttgg ggagaaggat
     1921 ttgaaacaga gatcaacaac cacctttata atataagagg tgcgttatct atggctcgct
     1981 cacaagatcc taacagtaac ggcagccagt tctttatcgt tcaaaataca gatgatatgc
     2041 atgatggtct tttaaaagac gactatcctc aagcaatcat cgacgcttat aaaaacggcg
     2101 gttatccttc actagatggc cattatactg tcttcggcca agtgattgaa ggaatggatg
     2161 tcgtggatgc tattgcaaat atcgaaacag atagttcaga caaaccaaaa gaagaagtaa
     2221 aaatcgataa aatcaatata ctgcaagaag caaaacaaaa ataaaaaaat aacgtctgaa
     2281 tcctgcatag atgacaacgt atgctaggat tcaggcgtct tctttttaca taaaaagcaa
     2341 gtaagcttcc ttttgttttg ctatatcagg ttttatagtc aagcattttg gttgacgatg
     2401 aacgtgaact cgcactcaca gactttttta tcatctacat atgctgcaga taacgctgta
     2461 ccgactgatc ccttcatttt ggtgatttca aaatgacatt tcagcacatc tcctggaaca
     2521 actttttgac ggaatttggc cttattgatc ccaccgatat aagctctttt tcctagaaac
     2581 tcttctgatt ttaggataag tatcgaacct gcttgtgcca gtgtttctag aatcaacgca
     2641 cccggcatca ctggattgtc aggaaagtga ccttggaaaa aatcttcatt gattgttaca
     2701 ttttttgtcg caacgatctt ttttcctggt tctaaagtat ctacataatc aatataacaa
     2761 ataggataac gattaggaat caattccatt acttcagtag cagataatac gtaagacata
     2821 attaaactcc ttctttcagg caattgccat tttcattact ttgactatca aagtatcata
     2881 ttcctaaata tttagcaatc tttttttaat ttaagaaaaa atagtttgac aatcaaaaca
     2941 ttttgactaa taattaagaa atcaagaagt tattttagct attattctct atttttttac
     3001 gactatcaaa tttttatcag aaacaaagga gagtcattat gtcatttcta gaaggaaaaa
     3061 aaatcgtcat tatgggcgtc gctaacaaaa aaagcattgc ttggggttgt gctgaagcaa
     3121 tgttgaaaca aggggcagaa attatctata cttaccaaaa tgaacgaatg aaaaaatcat
     3181 tagtcaaatt ggtcggcgaa gaagcattca ctgtagaatg tgatgtttct agcgacgaaa
     3241 gcatcgaaaa agcaatgaat gccatcggtg aatatgctgg ggagatccac ggccttgtcc
     3301 atgcagttgc ttacgccaac aaagaagaat tgtccggaaa tgtgtctgat atttccagag
     3361 acggctatca attagctcaa gatatcagca gctactcttt aattgctgta tcaaaatacg
     3421 cacaaccgtt tcttgcacca aacagcggga tcgttagcat gacttatcta ggttcagaac
     3481 gcgcaatccc gaattataac atgatgggta ttgcgaaagc ttctcttgaa gctgctatcc
     3541 gttatttggc agctgagttc tcgcctaaag gaattcgggt caatggaatc tcagctgggg
     3601 caatcaaaac attagctgtc acaggtgtta aagactatca aaaactgatc cagttatctg
     3661 aatctcgtac gcctgatcag caaggtgtga cgatcgaaga agtcggtaat gcttgtgctt
     3721 tcttaatcag cccactttcc agcggaatca tcggtgatat tatctacgtt gacaaaggtg
     3781 tccatctttc ataaactttt gttgcattct ttaaagtaaa acatataaaa atcaggaaaa
     3841 aactttttaa agtctcttcc tgattttttc tttttacgtt tttttcaact cattactttg
     3901 aaattattcc acgacttcaa tccagccttc tggtgcttct atgtctccaa attgaatacc
     3961 tgtcaattca tcatacaacc gttttgttac tggtcctact tccgtttcac tataaaatac
     4021 atgaagattg ttattgtgat aaatcccacc gattggagaa ataacagccg ctgtaccaca
     4081 tgcaccagct tctgcaaatt gatctagatc atcaatgaaa atatctcctt caattgcttg
     4141 cagtcctagt cgttcttcag ctaactgcaa caaggaatat ttcgtgatac ttggtaagat
     4201 agagggcgat tgcggagtga tgaattttcc gtcttttgta attccaaaga aatttgctgc
     4261 acctacttct tcgattttcg tatgtgtggc tggatcaaga taaacacaat ctgagtaatc
     4321 cagttctttt gcttctgttc ctggaagtaa actcgctgcg taatttccgc cgacttttgc
     4381 tgcaccagtc cccttaggag cagcacgatc gtaatcagat acaataaaat tcgttggttt
     4441 tagtccacct ttgaaatacg ctcctactgg cgtacagaag acgacaaata aatattctgg
     4501 agctggatgg acaccaatgt tttcaccgac cccaatcagt aaagggcgca agtataatgt
     4561 agcgcctgtt ccatagggcg gcacaaaatc ttcatttgct tttaccactt gcttgacagc
     4621 atcaacgaac atcttttcag ggaccattgg catcagcaat cgtctagcac tttgattcat
     4681 ccgtcgacta ttttgttcag gtcgaaagag attgaccttg ccgtcttttc gacgataagc
     4741 ttttagacct tcaaaacatt gctgaccata atgaagcgct ggagagcctt catgaatcgt
     4801 caggtaatta tcttctgtca attctccttc ttcccattgt ccgtctttcc atttggcaat
     4861 aaaacgccaa ggagttttga tataagaaaa acctagattg ttccaatcga tttgcatata
     4921 cttaccacct tcgttatttt ctccacattt tatcattatc catattattt gtcattttat
     4981 tttcagcttt tttccttcat tatagttatt tttgctattt ccttttttga aaacttcttg
     5041 gtacaataca gaaagaatca aattttagaa aagaggattt tatgttttca ttattagcaa
     5101 ctgccggtac gacagactct tctggcgaga cattgacaga tcaagctgtc agcaaagtaa
     5161 atgcgtttca gcggttttgg gacgggattg attgggacgc cattttggct gcctttatcc
     5221 aaaaaagttt atatctgatt tttttgatcg tccttttttg gattcttcac cgtgtgggaa
     5281 aatatctgat tgataagtct tttaatcagt attctaaaaa agccattcta aacgagagcc
     5341 gcttaaagac tttacatagt cttttaaata ctatttttca ttataccctt ggtttctttt
     5401 ttatctatgc aattttatct gctatcggtg tgccgatcgg atcacttttg gctggtgccg
     5461 gaattgcagg tgtggcaatc ggtcttggtg cccaagggtt catgagtgat gttattaccg
     5521 gattttttat tatcatggaa caacagatgg atgttgggga ctacatcaag ctagcaaact
     5581 taacgattga aggaaccgtc gtatctgttg gtatccgtac ccttcagctg aaagcaacgg
     5641 atggtactgt ccatttcatc ccgaatcgaa acatcaccac gatcagcaat ctctctcgtg
     5701 caaatatgca agtactttta gatattcgga ttgttcctga agaaggatat gacaaaatct
     5761 atgagatcat tgatcgagta aatcgtacat tagctgaaaa ataccaagaa gagctccaaa
     5821 ctgaaccttc tatctttgga ttagtggatc ttggaaacag caattttgcg attcgtacgg
     5881 tctgctacgc attgaacgga aaacaatatg ccttaaaaga agagttcttg agctcctatg
     5941 tcaaagaatt gacagcttct ggtttcacga ttccgaacag tccaattgct gtcggaaaat
     6001 aatttggaca aacgatgcaa cgaatctcac taaaaagagc gtgggacaaa agtaaa
//
LOCUS       DASHRQ010000106         5956 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.114, whole genome shotgun sequence.
ACCESSION   DASHRQ010000106 DASHRQ010000000
VERSION     DASHRQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.114"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(44..682)
                     /gene="plsY"
                     /locus_tag="U2M88_002660"
     CDS             complement(44..682)
                     /gene="plsY"
                     /locus_tag="U2M88_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEM8374984.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            809..1243
                     /locus_tag="U2M88_002661"
     CDS             809..1243
                     /locus_tag="U2M88_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEM8374985.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1409..3454
                     /gene="parE"
                     /locus_tag="U2M88_002662"
     CDS             1409..3454
                     /gene="parE"
                     /locus_tag="U2M88_002662"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEM8374986.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIKYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3466..5916
                     /gene="parC"
                     /locus_tag="U2M88_002663"
     CDS             3466..5916
                     /gene="parC"
                     /locus_tag="U2M88_002663"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEM8374987.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
BASE COUNT         2118 a         1025 c         1333 g         1480 t
ORIGIN      
        1 gatttgtcac actcccgttt tatctctttg gaatgaaaaa gtatcaggat tttttcttgc
       61 tgctattcaa accaaacggg attcgacttt cagtatggtt tctgatacgc ttgatattgt
      121 ctttatgccg ataaaagata aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat
      181 tgggcttagc caaaatcaca ggagctacat aaggaaggac gatcgtagat aaagtgatca
      241 atactgcact gatcatactg ctcaaactca ccatacttgt acaaaataaa ctaacgataa
      301 agatgataca agaataaaca aaaaatacag gattaaatcc taaaagcatt cctgcacttg
      361 tcgcaacagc ttttcctcct ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg
      421 cgcatacccc aaaccaaaga gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag
      481 tagccaatgt accttttaaa atatccataa gcaggacaac aatcccggct gttttgccta
      541 atacacgaaa tgtattggtc gttccagtat taccgctccc gtgttgtcgg atatcttttt
      601 tgaaaaacaa tttgccaatc cagacaccgg aaggaataga acctaaaaga tatgctaaaa
      661 taagtaataa gatcaatttc attttttacc ctctttcaag ttgaaaacta tcctttattt
      721 tagcatgaat aggtaacagg aacaattgaa aaaatgaaag atacaagtat aatagatgga
      781 ataaaaagaa aagcaagggg tggcagaaat gtctgtaaaa aatccaacac aagaaacgat
      841 tttcaactat ttggaagaag caaaaaatat tgccgtcgta ggcttgagca ataaaccaga
      901 acgaacaagt tacatggttg cacaggtatt gcaagacaat ggatataaaa tcataccagt
      961 aaatccatta ttggctggtg aagaaattct aggcgagaaa gtctatgcga atttgacaga
     1021 aatcgatgat ccaatcgata tcgtagatgt atttagacgg agtgaatttt tacctgaaat
     1081 agcaggtgat tttttgaaaa ctgatgctaa agtgttctgg gcacaattag ggattgaaaa
     1141 caaagaagca gctgagatgc taagaaaagc aggacgtaca gatatcgtaa tggatcgttg
     1201 tattaagatt gaacttggta agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag
     1261 agatgatcga tagaggggaa tgtcgaaaaa gcggttagga gataattaaa attgatttct
     1321 cgtctaccct cttttttttc acgacctttt ctagtatgat aatcgtggtg cggttttgcc
     1381 gcaaagtatt attgaaggag cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga
     1441 ctcgtccatc caagtattgg aaggattaga ggcagttaga aaaagaccag ggatgtacat
     1501 tggatccact gacagccgag gcttgcatca tttagtctat gaaatcgtcg ataatgcagt
     1561 tgacgaagca ttatcaggtt atggcagtga gattgatgtg accatccatg aagacaatag
     1621 tataactgtt gcagatagcg gacgtgggat gcctgtagga atgcatgctt ccggaatacc
     1681 aacagttgaa gtgattttta ctgtgctgca tgctggcgga aagtttggtc aaggagggta
     1741 caaaacttct ggtggactgc acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg
     1801 gctaattgta acgatcgtta gagatggcgt agagtatcag cagaaattta aaaacggcgg
     1861 taaacctgac ggtactttga aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt
     1921 ccatttttta ccagatgata caatattcag tacaacgaaa ttttcatatg aaattttagc
     1981 cgaacggtta agagaatctg cctttcttct aaaaggcgtt aaaatctctc tttctgatct
     2041 tcgtggagaa gaaccagtca aggaaatttt tcattacgaa gaaggaatca aagaatttgt
     2101 tgattatctg aatgaagaaa aagatacatt gacaccagtt gtttattttt caggagaaaa
     2161 agaaggtatc gaagtagagg tcgcttatca atacaatgac ggttattcag aaaatgtttt
     2221 atcttttgtc aataacgttc ggacaaaaga tgggggaacc catgaagctg gtatgaaagc
     2281 ggcgatgacg aaatcttata atgaatatgc gagaaaagtt ggtttgctga aagaacggga
     2341 caagaatctg gaaggtagtg atttcagaga agggttagct gctgtacttt ctatccgtgt
     2401 acctgaaaat cttctgcaat ttgaagggca aacaaaagaa aaacttggta cgccagttgc
     2461 tcgtaccgta gtagataacg tcatcagtga acaaatgggc ttttatttgc aggaaaatag
     2521 cgaaatgagc cagatgttgg tccgtaaagc aatcaaagcc agagaagcga gagaagcagc
     2581 acgtaaagcc agagaagaaa gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct
     2641 ttcaggaaaa ttgacacccg cacaatcccg aaaccctaag aaaaatgaat tgtatttagt
     2701 cgaaggggat tctgctggcg gatctgctaa acaaggaaga gacagaaagt tccaagcaat
     2761 tctaccttta cgaggaaaag tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa
     2821 aaatgaagaa atcaatacga tgatctatac gattggtgca ggagtcggtc ctgagttttc
     2881 tatcgaagac tgtaattacg acaaagtaat catcatgacc gatgcggata cagacggcgc
     2941 ccatattcaa gtattactgc ttactttctt ttatcgttat atgaaaccgc tgattgaagc
     3001 tgggaaagta tatatcgcgt taccgccttt atataaagta tccaaaggac aaggaaagaa
     3061 gcaagtcatt aaatatgcgt ggacagatga cgaattggca gcaatgatta aaaaagtcgg
     3121 caaaggatat atgctgcagc gatataaagg acttggagag atgaatgcgg aacaattgtg
     3181 ggaaacaacg atggatccga cttcccgtac gttgatccgc gtccgtatcg atgatgctgc
     3241 gcaagctgaa cgtcgagtaa ccacactgat gggtgacaaa gtagaacctc gaagaaaatg
     3301 gatcgaaaat cacgtgcaat ttacgctcga agaagatggc agtatcttag ataaaaaaga
     3361 ggatactgaa atctctcctt cagtttctaa tgatttatta gatgaagaac gagcagataa
     3421 aaatgaaaac aatcagttat ttgaagtaga ataggaggtc acctgatgga aaatcgacaa
     3481 gaagtacaag aattaacttt agaagaagtg atgggtgacc gcttcggacg ttattccaaa
     3541 tatattatcc aagaacgtgc actgcctgat atccgtgatg gattgaagcc agtgcaacgc
     3601 cggatattat ttgccatgaa caaagatggc aatacgtatg acaaaggatt ccgtaaatca
     3661 gcaaaatctg tcggaaatat catggggaat tatcatcctc atggtgaccg cagtatttat
     3721 gaagcgatgg tccgcatgag ccaagactgg aagttgcggg aagtactgat cgaaatgcac
     3781 gggaataacg ggagtatgga cggtgatcca cctgctgcga tgcgttatac tgaagccaga
     3841 ttatccaagt taagtggaga gatgctagca gatatcgaga aagaaacagt cgatctcgtg
     3901 tggaattttg atgatacaga aaaagaacca acagtccttc cagctcgata cccaaacctt
     3961 ctcgtcaatg gatcgacagg tatatctgct ggatatgcaa cagaaatccc gactcataac
     4021 ttagctgaag tgatcgatgg cacaatctat atgatcgatc atccacaagc gagccttgaa
     4081 aaattgatgg aatatattcc tggacctgac tttccaacag gagggatctt gcaaggaaaa
     4141 gatgaaatca aaaaagctta tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca
     4201 aagatagagg caatgaaggg taataaacag caaatcgtga tcacagaaat cccatatgaa
     4261 gtaaataaag cgaccttggt gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat
     4321 ggaatagctg aagtacgaga tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg
     4381 aaaaaagacg ccaatgctca aggaattttg aattatttgt tcaaaaacac tgagctacag
     4441 atcaattaca atttcaatat ggtcgctatc gaccatatga ctccgcatca agttggactg
     4501 aaagacattt taagaagtta tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa
     4561 ttcgatttag ccaaagctca aaaaagacag catatcgtag aaggattgat gaaagcttta
     4621 tcgattctgg atgaagtaat cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa
     4681 aagaatttag tcgacgtctt tcaatttaca gaagaacaag cagaagcaat tgtcacattg
     4741 caattatacc gtttgacgaa cacggatatc acagagcttc aaaaggaatc cgaaagcttg
     4801 attgcgcaaa tcacagagtt gaataaaatc ctttcaaatg acaaagagct tttctctgtt
     4861 atgaaaaaag aattgcgaga agtgaaaaaa aattattctt ccgctcgtct aacgacaatc
     4921 gaagacgaaa tcgaagaaat caaaattgat acgcaagtct tagtggcaca agaagacgta
     4981 attgtcagtg tgacgcgaga aggctatgtg aagcgaacaa gccttcgttc ttatagtgct
     5041 tccaaaccag aagaaatcgg gatgcgagaa ggcgactact tgctatactc aggtgaattg
     5101 agtacattgg accatgttct tctgatcaca aataaagcaa atgtgatcta ccgccctgtc
     5161 catgaactac ccgatttgaa atggaaagat gctggtgagc acatttctca aacaatctcc
     5221 aatctgtctg tagacgaatc gattttagct gttttcccat atcaaaaaat tcaagcagag
     5281 aaaacatttg tattcatcag caagaatgga ttgatcaagc aaacacgaat gacagagttc
     5341 gaaccatggc gcacctataa gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa
     5401 gatgaactga ttgctgtgta tctgaaagaa gatcagccag gcttagatgt tttcttagta
     5461 acgcatcaag gaatgggttt gcgctatcct ttaacagatg ttcccgttgt tggagcaaag
     5521 gcagctggag tgaagtctat gaatttgaaa gatggagata ggattatcaa cgggctgctt
     5581 gtgttagcag aaggtgatac gccgatcgta atcgttactc agcgaggagc tgttaaacgc
     5641 atgcttgctc aagaaatcag ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt
     5701 gagctgaaaa aaaatccgca ccgtattatc tatatgtcgg aaggaagatc aaaagaaata
     5761 accttgatga atcaaaaagg gcagcagtta acgattgacc caactgattt ccctataggt
     5821 gatcgaacat cgaatggatc atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa
     5881 gttatcgatg ctcctgagat tttcatcgag tcgtaactgt tgtagtattt taatgaagag
     5941 acgccatctg tattaa
//
LOCUS       DASHRQ010000107         5940 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.115, whole genome shotgun sequence.
ACCESSION   DASHRQ010000107 DASHRQ010000000
VERSION     DASHRQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.115"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            704..1438
                     /locus_tag="U2M88_002664"
     CDS             704..1438
                     /locus_tag="U2M88_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM8374988.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1454..2623
                     /locus_tag="U2M88_002665"
     CDS             1454..2623
                     /locus_tag="U2M88_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEM8374989.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2639..3634
                     /gene="lacD"
                     /locus_tag="U2M88_002666"
     CDS             2639..3634
                     /gene="lacD"
                     /locus_tag="U2M88_002666"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEM8374990.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3713..4639
                     /locus_tag="U2M88_002667"
     CDS             3713..4639
                     /locus_tag="U2M88_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEM8374991.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4658..5908
                     /locus_tag="U2M88_002668"
     CDS             4658..5908
                     /locus_tag="U2M88_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM8374992.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1992 a          967 c         1213 g         1768 t
ORIGIN      
        1 tagcatacgg aaagaagttc tgcaccttat aaaatttgtc aaaaaaactg ttgccattcc
       61 aaagtttgaa aaatgctatt tcgaggtaga aggatattat ataagcacgg ttgactaaac
      121 gaagtgataa taaaaaaagt tattcaagaa taagaaaaat tatgatacga attcagataa
      181 actggagaat acagagacct ttaaaaggga taagaaacaa cataaattcc tcttaagaga
      241 gaacgaaagg acaaagccag caacttgata cacgagctag tattagagga atttattctc
      301 atagactccc ttttgaatac gtttcaactt taattttcat gttcataaat gatttgaaac
      361 caaagcaaat gcgttgcact tttcctccgt cttccgaaaa tcgttcaatt cactggtaag
      421 tagtcatttg atagtttgag gcaaattgtt gattgatgaa gggtaggagc taaaaaaaga
      481 taccagttga agagagaaaa agaaaaaaat agagggtgtt cttgaatgaa aaaagttgga
      541 aaattatttt tcatttcctt ttattcctat taaagtcagt gatgtcaatc atattaacaa
      601 gactaaaaat gaaaataaaa aaaagattgt tcttagtatt gactcataaa aaataccact
      661 actatactta cattgtggtt atagccagtt aggagagaca tcgatgcaaa atacctttca
      721 atcaaaatca ctttattatc agctggtaga aatattgaaa caacgaatcg aaaacagtat
      781 ggtcgctcat gacaagctgc catcagaaag agagttgacc agtttttatg gcgttagtcg
      841 cacgacggtg cgactagcat tgcaagaact ggaaaaaatg ggctacattt atcgacaaca
      901 tggaaaagga acctttgttt cggaattaag gacaaatgcg actgatctag ctggcgcata
      961 tagttttacc gaacaaatgc gggcaatggg caaacatcct gaaaccaaga tattggcctt
     1021 caagaagatt gaagcaacta aatatttagc gagtcatctg aatgttcaac taggtgagcc
     1081 tttatttcaa ttagaccgat tgcgcttagc tgatgatttg ccaatgatga tcgagaaatc
     1141 ctatttgcca gtgaagctat ttatgtccct ttctcaagaa attctatttc tgaaaccttt
     1201 atataatttg ttttcagaga atttcaatca aatcgtacgg ttggctgacg aggaatttta
     1261 tgccagcatt gtccagaaag aagatagtga gttgctaaat attccagaag gatcaccagt
     1321 gctccattta gttcgctcaa cctataatct cgagaacgaa gtgattgaat ttacattaag
     1381 tgtagcgcgg gctgataaat ttcattataa gatcaggcat ctgcgttcca ctaactgaat
     1441 tggaggaaag aaaatgttta tgttctcaaa agaagaacta cgaaagctag gagcagaaaa
     1501 tactacccga gaaattcgac agcaaccgga actgtggcat gagacgtttg accgttatca
     1561 agaaacagtt gcagatttag atcgtttgtt tacggatgcg aaacaacatg ctggagagcg
     1621 accaatccga gtcattttta caggggccgg aacctcgcaa tatgtgggag atacactaat
     1681 tccgtttctt actagtcaag gaaatacgaa ccagtttatt tttcaaagta ttggttcgac
     1741 cgatatcgtt gctacgccaa aagaatacct gattcctgat gagacaacga tcttggtctc
     1801 atttgcccgt agtggaaata gtccagaaag tctagccgca gttaagatgg ccaatcagct
     1861 ggtcaacaag atctatcata tcgtgatcac ctgtgcacca gacggattat tggctacaca
     1921 aatgcctaca aaaaaagaaa atctagtttt actaatgcca aacaaagcca atgacgctgg
     1981 ttttgctatg acaagcagtt tcagctgtat gacgctgact gcgctactta tttttgatca
     2041 agcaagcaat cagcaaaaag aaagatatgt tcatcaatta agccgactag gagaagaagt
     2101 catccaacga gaatcagaga tccaagggta tctggcatca gactttcatc gcatcattta
     2161 cttaggttcg ggaagcttag gcggattgac tagagaagcg caattaaaag ttctcgagtt
     2221 aactgctgga aaaatcgcaa ccgtatttga tacatcttta ggattcagac atggtcccaa
     2281 atcttttgtc aatgagcaaa cattggtttt cctgtttgtc agcaatgact catatacgca
     2341 gaaatacgat gtagacatgc tggaagaaat caaaaatgac ggtattgccc aacgcgttgt
     2401 tggaattggt caaaagggtt cattgtttag tggtgattcc tttgaattag ttgcagattc
     2461 gttactgccg gatggctatc ttgctttagc agatatcatg gtagcccaaa cgatcgcatt
     2521 actttgttcg gtgaaggttg gtaatacacc agatacacca tcgccaagtg gaacagtcaa
     2581 tcgtgtggtc aaaggcgtaa cgatttatcc atatgaaaaa taggaaagag tgtgaagcat
     2641 gttagaatgg tcggaaaata aaagacaagc aatggaacgt ctatcgacgc cagaaggcat
     2701 tattaccgct ttggcgatcg atcagcgagg cgcgctgaag aaaatgatgt cggctttagg
     2761 gatggaagcg acaaaggaaa aaattgagca gtttaaaaca ctaatttccc aagaattgac
     2821 tccttatgcc tcagcaattt tattggatcc tgaatacgga ctgcctgctg cacagaagcg
     2881 tgcagcagcg agcggtctgc tgttggcata tgaaaaaact ggttatgatc aaaccgtacc
     2941 cggccgcttg ccggatctgc tgtcggactg ttcagttctg cgattgaaag agttaggtgc
     3001 ggatgcggtg aaatttttac tgtattacga ttatgaagaa gcagaggaaa tcaatcggca
     3061 aaagcaagtt tttgttgaac gaattggaag cgaatgcatg gctgaaggac tgccatttta
     3121 tttagagtta ttgtcatatg atgctaaaaa tgtagatacg aaatcgcttt cctatgccaa
     3181 agcaaaaccg cgcaaagtga tcgaaatgat gaaagaattc tcgaaaaccc agtataaagt
     3241 agatgtctta aaggttgaag taccagtaaa catgtcattt gttgaaggaa atgcaacagg
     3301 ggagatcgcc tatacacgtc aagaagctgc cgcttatttt aaagaacaaa gcgatgcaac
     3361 agatttgccg tatatcttct taagtgctgg ggtatcagcg gcattattca tggagacatt
     3421 gcgttttgcc aaacatgctg gggcacgatt caacggtgta ctctgtggca gagcaacttg
     3481 gaaagatgca gtcgctcctt tcgttgaaaa aggagaagcg gcaactttag aatggctgac
     3541 agaacaaggg acacaaaaca ttcgtcaatt gaatgaagtg ataagagaaa ctgccatccc
     3601 atggtatgag aaagtaagtg aaagcacttg ttaatgtttc taatatatta gtagtgataa
     3661 gagatatgtg tgattataat ttatcattat tgaaactggg aggatatttt atatgaataa
     3721 aagattgtta gattattcag cttcagattt tttgaaagct aagccgatgg aattaaagca
     3781 ggcaattttg gcttctgaag gtagaacaat tatggcagaa aatgtgccaa gtagctgtcc
     3841 ttacatggga aaatcagtga caaatgcaga attggagaga gctgctggag ctgatttaat
     3901 tttgtttaat gcactagatc tttttaatcc tcaaatttca ggtgtacctt taactctaaa
     3961 agagaatcca ataatctgga ttaaaaaagc tttaggaaga gctattggtg taaatttaga
     4021 acctgtagac aaagaagttt tgatgagtga aaatcgaact gaaatttcaa gtggtagaat
     4081 cgtttctcct gagactttaa aaaaggcaaa tgaattaggc tttaatttta tttgtttaac
     4141 tggaaatcca ggaacaggag tatctaatag aactattgag aaagcaataa cattatccaa
     4201 atcaattttt aatggattaa ttatcgctgg aaaaatgcat ggtgctggtg ttgatgaacc
     4261 aattatgaac ttagatatag caaaaagatt tattaaaaca ggtgtagata ttttattagt
     4321 tcctgctcca tacactgttc cgtatttcat ggaaagtgat ttgcgaggaa tcgttgatta
     4381 tataaaagat tttaatagaa ataaatctat tgaggaaaaa gttctagtac taacggctaa
     4441 tggaacaagc caagactctt ctgaccaaga aactattaaa aaaattgcat tagcatcaaa
     4501 agcatgtgga gctgatattc aacacatagg ggattcttta aatggtattt gtcttccaga
     4561 aaatattttt gctttgggtc aagcaattcg gggatatcgt catcaaatta ttatgttagg
     4621 aaaatctaat tatcgttaat taaggaggaa atgaaagatg atcgacaaag ttcaaaaaat
     4681 agctgcaaaa attagtaatc aaagacatct actagcttta agagacagca tggctgcagt
     4741 tatgccatta atcattattg gttccttttt tatgttatta gcaaatttcc cgatagaagg
     4801 atttacaaaa tggttatctg aaattggagt acaatccttt ttgaacaaag catcagattc
     4861 aacatttggt attattggat tagctgttac ctactctttg ggacacaatc tagcaaagac
     4921 ttatggtgta gactcattat cagcaggatt attatctttg agtggatatg ttttacttac
     4981 acctttattg gatgcagatg gttctatagg atttccaaca aaatactttg gtacctcggg
     5041 tctttttgtt ggtattattg tagcacttgt atctacagaa attttccggt ggtttgtact
     5101 taaaaatatt acaattaaaa tgccggcaag cgttccacca aatgtaagcc gagctttcgt
     5161 tgctattatt cctggctttt ttgtcgttct actatggttt attgttgttg taatttgtta
     5221 taaacttgga attgaaaatg ttcatgctct tattgcagat acactcgcaa aaccactatc
     5281 tttactgacg aaaactttac caggaattat attggttata cttattcaat gtttcttttg
     5341 gatgtttggt attcatggtg cacaggtaac ggggccaata attgaaccgt tattattgca
     5401 aaactctgat gtaaatagaa ttgcttatca agctggaaaa gaacttccta acattataac
     5461 ttatgaattc ttatataact ttgttttttc tggtggtgca ggttgtttat ttgcacttgc
     5521 cattttaaca tttttctttt caaagagtca ggaaaataaa actttaggta agttatcaat
     5581 cgctccagta agttttcaag tagcagagcc ccttctattt gggtttccaa ctatattaaa
     5641 tataaaaatg ttaattccat ttgtaactgc accagtagta acaacattaa ttacttattt
     5701 ttcaatgagt atgggattag ttgcaaaacc tgtaggagca acaatcccat ggacaactcc
     5761 accgataatt gccggatttt tagcttctgg tggacgaata agtggtgcag tgattcaagt
     5821 aataacaatt gcaataaatg ttttgatata ctatccattc tttaaattag atgataatgc
     5881 caagctaaaa agcgaaaaaa atgattagaa ctacctatat gtttctaact gtagttaagt
//
LOCUS       DASHRQ010000108         5681 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.116, whole genome shotgun sequence.
ACCESSION   DASHRQ010000108 DASHRQ010000000
VERSION     DASHRQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.116"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            133..1287
                     /locus_tag="U2M88_002669"
     CDS             133..1287
                     /locus_tag="U2M88_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEM8374993.1"
                     /translation="MEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSIN
                     GELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQ
                     REMLLDALLEISDSDPLLQYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIELKE
                     PTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSF
                     QNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKA
                     GTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEY
                     RSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            1385..2758
                     /gene="tet(L)"
                     /locus_tag="U2M88_002670"
     CDS             1385..2758
                     /gene="tet(L)"
                     /locus_tag="U2M88_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HEM8374994.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            3322..4584
                     /locus_tag="U2M88_002671"
     CDS             3322..4584
                     /locus_tag="U2M88_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEM8374995.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            4829..>5681
                     /locus_tag="U2M88_002672"
     CDS             4829..>5681
                     /locus_tag="U2M88_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HEM8374996.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1954 a          805 c         1167 g         1755 t
ORIGIN      
        1 tccttgtacc ctgtttatca tggaagcgca aaaagcaaca tagggattga gcagcttata
       61 gaagtgataa cgaataaatt ttattcatca acatacagaa agaagtctga actttgcgga
      121 aatgtcttca aaattgaata ttcggaagaa agacaacgtc ttgcatatgt acgcctttat
      181 ggcggaatcc tgcatttgcg ggattcggtt agaatatcgg aaaaggaaaa aataaaaatt
      241 acagaaatgt atacttcaat aaatggtgaa ttatgtaaaa ttgataaggc ttattccggg
      301 gaaattgtta ttttgcaaaa tgagtttttg aagctaaata gtgttcttgg agatacaaag
      361 ctattgccac agagagagag aattgaaaat ccgctccctc tgctgcaaac aactgttgaa
      421 ccgagcaaac ctcaacaaag ggaaatgtta cttgatgcac ttttagaaat ctccgacagt
      481 gacccgcttc tacaatatta tgtggattct acgacacatg aaattatact ttctttctta
      541 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
      601 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
      661 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
      721 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
      781 tcatttcaaa atgcagttat ggaagggata cgctatggtt gcgaacaagg attatatggt
      841 tggaatgtga cggattgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
      901 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
      961 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
     1021 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
     1081 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
     1141 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
     1201 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
     1261 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
     1321 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggaatt
     1381 aattatgaag tgtaatgaat gtaacagggt taaatacaac caaattttaa tttggctttg
     1441 cgttttatct ttttttagcg tattaaatga aatggttttg aacgtctcat tacctgatat
     1501 tgcaaatgat tttaataaac cacctgcgag tacaaactgg gtgaacacag cctttatgtt
     1561 aaccttttcc attggaacag ctgtatatgg aaagctatct gatcaattag gcatcaaaag
     1621 gttactccta tttggaatta taataaattg tttcgggtcg gtaattgggt ttgttggcca
     1681 ttctttcttt tccttactta ttatggctcg ttttattcaa ggggctggtg cagctgcatt
     1741 tccagcactc gtaatggttg tagttgcgcg ctatattcca aaggaaaata ggggtaaagc
     1801 atttggtctt attggatcga tagtagccat gggagaagga gtcggtccag cgattggtgg
     1861 aatgatagcc cattatattc attggtccta tcttctactc attcctatga taacaattat
     1921 cactgttccg tttcttatga aattattaaa gaaagaagta aggataaaag gtcattttga
     1981 tatcaaagga attatactaa tgtctgtagg cattgtattt tttatgttgt ttacaacatc
     2041 atatagcatt tcttttctta tcgttagcgt gctgtcattc ctgatatttg taaaacatat
     2101 caggaaagta acagatcctt ttgttgatcc cggattaggg aaaaatatac cttttatgat
     2161 tggagttctt tgtgggggaa ttatatttgg aacagtagca gggtttgtct ctatggttcc
     2221 ttatatgatg aaagatgttc accagctaag tactgccgaa atcggaagtg taattatttt
     2281 ccctggaaca atgagtgtca ttattttcgg ctacattggt gggatacttg ttgatagaag
     2341 aggtccttta tacgtgttaa acatcggagt tacatttctt tctgttagct ttttaactgc
     2401 ttcctttctt ttagaaacaa catcatggtt catgacaatt ataatcgtat ttgttttagg
     2461 tgggctttcg ttcaccaaaa cagttatatc aacaattgtt tcaagtagct tgaaacagca
     2521 ggaagctggt gctggaatga gtttgcttaa ctttaccagc tttttatcag agggaacagg
     2581 tattgcaatt gtaggtggtt tattatccat acccttactt gatcaaaggt tgttacctat
     2641 ggaagttgat cagtcaactt atctgtatag taatttgtta ttactttttt caggaatcat
     2701 tgtcattagt tggctggtta ccttgaatgt atataaacat tctcaaaggg atttctaaat
     2761 cgttaaggga tcaactttgg gagagagttc aaaattgatc ctttttttat aacaggaatt
     2821 caaatctttt tgttccatta aagggcgcga ttgctgaata aaagatacga gagacctctc
     2881 ttgtatcttt tttattttga gtggttttgt ccgttacact agaaaaccga aagacaataa
     2941 aaattttatt cttgctgagt ctggctttcg gtaagctaga caaaacggac aaaataaaaa
     3001 ttggcaaggg tttaaaggtg gagatttttt gagtgatctt ctcaaaaaat actacctgtc
     3061 ccttgctgat ttttaaacga gcacgagagc aaaacccccc tttgctgagg tggcagaggg
     3121 caggtttttt tgtttctttt ttctcgtaaa aaaaagaaag gtcttaaagg ttttatggtt
     3181 ttggtcggca ctgccgacag cctcgcagag cacacacttt atgaatataa agtatagtgt
     3241 gttatacttt acttggaagt ggttgccgga aagagcgaaa atgcctcaca tttgtgccac
     3301 ctaaaaagga gcgatttaca tatgagttat gcagtttgta gaatgcaaaa agtgaaatca
     3361 gctggactaa aaggcatgca atttcataat caaagagagc gaaaaagtag aacgaatgat
     3421 gatattgacc atgagcgaac acgtgaaaat tatgatttga aaaatgataa aaatattgat
     3481 tacaacgaac gtgtcaaaga aattattgaa tcacaaaaaa caggtacaag aaaaacgagg
     3541 aaagatgctg ttcttgtaaa tgagttgcta gtaacatctg accgagattt ttttgagcaa
     3601 ctggatccag gagaacaaaa acgatttttt gaggaaagtt ataaattatt ttccgaacga
     3661 tatggcaagc aaaatattgc ttatgcaaca gttcataatg atgagcaaac ccctcacatg
     3721 catttaggtg ttgtgcctat gcgtgatgga aaactgcaag gaaaaaatgt gtttaatcgt
     3781 caagaactgt tatggctaca agataaattc cccgagcata tgaaaaaaca gggttttgag
     3841 ttgaagcgtg gtgaacgtgg ctctgaccgt aaacatattg agacagctaa atttaaaaaa
     3901 caaactttgg aaaaagagat tgattttcta gaaaaaaatt tagcagttaa aaaagatgaa
     3961 tggactgctt atagcgataa agttaaatca gatttagaag taccagcgaa acgacacatg
     4021 aaaagtgttg aagtgccaac gggtgaaaag tccatgtttg gtttgggaaa agaaataatg
     4081 aaaacagaaa agaaaccaac caaaaatgtt gttatatcgg agcgtgatta taaaaactta
     4141 gtgactgctg cgagagataa cgataggtta aaacagcatg ttagaaatct catgagtact
     4201 gatatggcga gagaatataa aaaattaagt aaagaacatg ggcaagttaa agaaaaatat
     4261 agtggtcttg tagagcgatt taatgaaaat gtaaatgatt ataatgagtt gcttgaagaa
     4321 aacaagtctt taaagtctaa aataagcgat ttaaagcgtg atgtgagttt aatctatgaa
     4381 agcactaagg aattccttaa ggaacgtaca gacggcttaa aagcctttaa aaacgttttt
     4441 aaggggtttg tagacaaggt aaaggataaa acagcacaat tccaagaaaa acacgattta
     4501 gaacctaaaa agaacgaatt tgaactaact cataaccgag aggtaaaaaa agaacgaagt
     4561 cgagatcagg gaatgagttt ataaaataaa aaaagcacct gaaaaggtgt ctttttttga
     4621 tggttttgaa cttgttcttt cttatcttga tatatataga aataacgtca tttttatttt
     4681 agttgctgaa aggtgcgttg aagtgttggt atgtatgtgt tttaaagtat tgaaaaccct
     4741 taaaattggt tgcacagaaa aaccccatct gttaaagtta taagtgacca aacaaataac
     4801 taaatagatg ggggtttctt ttaatattat gtgtcctaat agtagcattt attcagatga
     4861 aaaatcaagg gttttagtgg acaagacaaa aagtggaaaa gtgagaccat ggagagaaaa
     4921 gaaaatcgct aatgttgatt actttgaact tctgcatatt cttgaattta aaaaggctga
     4981 aagagtaaaa gattgtgctg aaatattaga gtataaacaa aatcgtgaaa caggcgaaag
     5041 aaagttgtat cgagtgtggt tttgtaaatc caggctttgt ccaatgtgca actggaggag
     5101 agcaatgaaa catggcattc agtcacaaaa ggttgttgct gaagttatta aacaaaagcc
     5161 aacagttcgt tggttgtttc tcacattaac agttaaaaat gtttatgatg gcgaagaatt
     5221 aaataagagt ttgtcagata tggctcaaag atttcgccga atgatgcaat ataaaaaaat
     5281 taataaaaat cttgttggtt ttatgcgtgc aacggaagtg acaataaata ataaagataa
     5341 ttcttataat cagcacatgc atgtattggt atgtgtggaa ccaacttatt ttaagaatac
     5401 agaaaactac gtgaatcaaa aacaatggat tcaattttgg aaaaaggcaa tgaaattaga
     5461 ctatgatcca aatgtaaaag ttcaaatgat tcgaccgaaa aataaatata aatcggatat
     5521 acaatcggca attgacgaaa ctgcaaaata tcctgtaaag gatacggatt ttatgaccga
     5581 tgatgaagaa aagaatttga aacgtttgtc tgatttggag gaaggtttac accgtaaaag
     5641 gttaatctcc tatggtggtt tgttaaaaga aatacataaa a
//
LOCUS       DASHRQ010000109         5579 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.117, whole genome shotgun sequence.
ACCESSION   DASHRQ010000109 DASHRQ010000000
VERSION     DASHRQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.117"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1350
                     /locus_tag="U2M88_002673"
     CDS             <1..1350
                     /locus_tag="U2M88_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEM8374997.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..2827
                     /locus_tag="U2M88_002674"
     CDS             1484..2827
                     /locus_tag="U2M88_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEM8374998.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2939..4390
                     /locus_tag="U2M88_002675"
     CDS             2939..4390
                     /locus_tag="U2M88_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEM8374999.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            4739..5371
                     /locus_tag="U2M88_002676"
     CDS             4739..5371
                     /locus_tag="U2M88_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEM8375000.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT         2150 a          778 c         1073 g         1578 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2761 tcctagtttt agccaaccct ataattttat tttaaaggca gtaacaggat taaacattga
     2821 aacataatcg ttactaaatg aaatcaagaa atatactgta ttactcaatt cattgaaaat
     2881 atttatcata atttttaata gaaaacacta ttaaagagaa aaaagagagg taaatcaaat
     2941 gaaaaaatct ttaaaacttg catcagcagt tatattaatg tcccaactcc ttatagcaac
     3001 acctttccat gtgttggcaa gcgaaaattc aacatacaaa attgcaaaag aaagaaccga
     3061 gacgactgaa atgagtaatt ccgatgtaac agcagagagt agtgaacttt tgacaacttc
     3121 atcaagtgaa agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag
     3181 tagtacgact tcaaatgccg agtcttcaac aacttcatca agtgaaagta gtacgacttc
     3241 aaatgccgag tcttcaacaa cttcgtcaag tgaaagtagt acgatttcaa gtaccgaatc
     3301 ttcaacaact tcaagtagta caagtgaaat aacttcttcg aaagtgacaa aaccaaacca
     3361 tatagaatct tcaaaaaagg aagcgccaaa aacggaagga caaacaaaaa ctttttcgac
     3421 accacagcaa atagaatcaa cagaatcttt ccaatcacaa ccaatactat ctggtaatga
     3481 agaaattcat tttgacaaaa atgaatctgt agaaaatttt attatgaaaa ttggtgagtc
     3541 tgcgagaaaa attggtcaga aatatgattt atatgcttct gttatgattg cacaagcgat
     3601 cttagaatct gccagtggtc aaagccagtt agctcaggca ccgaattaca atttatttgg
     3661 gataaaagga acttataatg gaaactttgt cattatggta acaaacgaag atttgggtaa
     3721 tggaacgctt tatacgaccc aatcaaaatt tagagtgtat gaaaactatg aggaaagctt
     3781 tgaagattat gccaaattat taactaaggg aattagtgga aataaagatt tttatgcagg
     3841 agcattaaaa gctaatagta aaacatatcg agaagcaact aaattcctta caggacggta
     3901 tgcaacagat acacaatact atttgaagct aaatgaatta attaaaacct acgatttaac
     3961 caactatgat aaagaagtta aaggtccaca aatgagtaag actggatacc aagtgcctat
     4021 tcataattat tcaataacca gtttgtttgg aaatcgtgga aatgaatttc accgaggact
     4081 tgatatggca gctacacagg gtgagccgat tcatgcagtt aaagctggta aggttgttaa
     4141 agcagagttt cactactctt ggggaaatta cgtggtgata caacacgaag atggatcaac
     4201 cgcgttatat gctcaccagc aacaatacct agtaaaaact ggagataatg ttttgcaagg
     4261 acaaataatt ggctatgtag gaagtactgg gaatagtact ggcccacacc ttcatttgga
     4321 aatttgcaaa gatagtagtc tttctcagag catgttagtt gatccgaaaa ccttattatt
     4381 cggagactaa aaaaggtaat caatctgaca aagtaacaag aaaatggttg gattcttaga
     4441 cctttgatca ggatattgag actggaattg atatctcgat tacgatgggt ttgacaaata
     4501 aaacaagtcc attatcgaat atcgagtgta ttattgccgt ctttatgctc atattttgag
     4561 caggtttcac tagatgaaaa caatttatct atcttgagga tttcgtgtac gtatcgttca
     4621 gtcttgtatt gtaaattagt cataaaatca gactaagaaa aattggaaat atttttttct
     4681 gatttattaa aagttaattg aaattatatg gtacttcttt cttgggaggg taggtgcagt
     4741 gaaggaaaga tatggtcacc ttactgttgt tgaggagatt actcataatg gatataaaaa
     4801 atatcgttgc ctatgtgact gtgggaatga aacgattgta tatcgatcaa acttactatc
     4861 aggtagaacc aaaagttgtg gatgtggaaa ttataaaaat aggcataaat ataaaaattt
     4921 gacaaataaa acttttggta agttagttgc aatcaagcca actaaactgc gaagtgaagg
     4981 aactgttgtt tgggaatgtc aatgtctgtg tgggagaaaa aagttagtat cggcaaagcg
     5041 acttctaaaa ggtgatgtaa aaagctgtgg atgtttgaaa tcaaaaaagc atcacattga
     5101 aaataaaaaa ttcggattac ttactgcaaa gtatcctttg gacaataact gttcatggaa
     5161 aacaaaatgg gtttgtcatt gtgagtgtgg cagtatgtgt gagataagtt atagtaactt
     5221 attttttgga catactcaaa gttgtggatg tttaaaaagg caagaaacaa ggacatgtgt
     5281 tgaagggaca gtaatagagt gtctaacgtc aaaattaccg aaaaataata caagtggtgt
     5341 taagggagtt tatcgttcta aagagaaata gattgcgtat ataactttaa aaaaaaagag
     5401 gtactattta ggatcgtatg attctattaa gagtgcagct caagctagaa aagatgctga
     5461 aaataaattg tttcaacctt tgattgacaa atatcgaggt aatcattaat tattgtggat
     5521 aataattaac agtgatatat aaacatctat aatatattat tcatactatt gatatttag
//
LOCUS       DASHRQ010000110         5463 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.118, whole genome shotgun sequence.
ACCESSION   DASHRQ010000110 DASHRQ010000000
VERSION     DASHRQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.118"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..933
                     /locus_tag="U2M88_002677"
     CDS             <1..933
                     /locus_tag="U2M88_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM8375001.1"
                     /translation="RWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLA
                     IDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHI
                     ESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAM
                     LIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVT
                     QNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCL
                     ISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            936..3119
                     /locus_tag="U2M88_002678"
     CDS             936..3119
                     /locus_tag="U2M88_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEM8375002.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3116..4117
                     /locus_tag="U2M88_002679"
     CDS             3116..4117
                     /locus_tag="U2M88_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEM8375003.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4132..5046
                     /locus_tag="U2M88_002680"
     CDS             4132..5046
                     /locus_tag="U2M88_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM8375004.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5291..5407
                     /locus_tag="U2M88_002681"
     CDS             5291..5407
                     /locus_tag="U2M88_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEM8375005.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         1727 a         1048 c         1154 g         1534 t
ORIGIN      
        1 agatggaaag aaaccttgcc agagatttcc catgaaatca atatcgtcaa tctgacttct
       61 gatgagaaaa acaaaggctt acttgaccct tatgtaatta tgaaaaatac caaagattct
      121 gaatcactgg ctattgatat tttgacattc cttacgggga tttcctctcg tgatggggaa
      181 cgcttcccaa tccttagaaa agccattcgt gcagtaacca atagtgaagt gcgagggttg
      241 atgaaagtga ttgaagaatt acgggttgag aatacgccac taagtaccag catagccgac
      301 catatcgaaa gttttacaga ctatgacttt gcccatctgc tttttagtga tggttatgta
      361 gagcagtcta tcagccttga aaaacaactg aacattatac aggttgccga cttggtactt
      421 cctgacaagg aaacttcctt tgaggaatat accacaatgg agttactatc ggtagcaatg
      481 ctgattgtca ttagtacctt tgcgttggac tttatccata cagaccgaag tattttcaag
      541 attgtagact tagacgaagc atggagcttc ttacaggtgg cacaagggaa aacactatct
      601 atgaagctgg ttcgtgctgg tcgtgctatg aacgctgggg tatatttcgt gacccaaaat
      661 acagatgacc tcttagatga aaaactgaaa aataacctcg gcttaaaatt tgcctttcgt
      721 tccactgacc ttaacgagat taaaaaaacc cttgcctttt ttggtgtaga cccagaggac
      781 gaaaacaatc agaagcgatt gcgtgatttg gaaaacgggc aatgccttat cagtgattta
      841 tatggtcgtg tcggtgtgat acagttccac cctgtatttg aagaactgct ccatgccttt
      901 gataccagac cacctgtgcg aaaagaggtg taaatgtgaa accatcaata ttaaacagaa
      961 taaaatcaaa ctggacgctg aaacgtctag gtaaagtggc aatgacagtg gctttcacac
     1021 ttgtgattgc catttttctt ttagccatgc tgggaacggt ggttcaagct gcgggcttgg
     1081 tagatgatac ggtcaatgtg gcaaatgaat acagccgata cccacttgaa aactatcaac
     1141 tggattttta tgtggataat agctggggct ggctaccgtg gaactggtcg gacggaattg
     1201 gaaaacaagt catgtatgga ctatatgcca ttaccaattt tatttggaca atcagtcttt
     1261 atgtttccaa tgcgacgggt tacttagtac aggaagccta ttccttagac ttcatttccg
     1321 ctacagcaga ttccattggc aagaatatgc agacccttgc aggggttacg cctagtggac
     1381 tatcaacaga gggtttctat gttggattcc ttttactctt gattttggtt cttggggttt
     1441 atgttgccta tacaggactg ataaagagag aaaccacaaa ggcaattcat gccctcatga
     1501 attttgtgct ggtttttatc ctatcggctt cctttattgc ctacgctccc gactacatta
     1561 aaaaaatcaa tgacttttca tcagacatca gcaacgctag tttgtcactg ggtacaaaaa
     1621 ttgtcatgcc ccattcagat agtcaaggca aggacagtgt ggacttgata cgtgacagcc
     1681 tattttccat acaggttcag caaccgtggc tactgcttca atataacagt tcagacattg
     1741 aaagtattgg tattgaccgt gtggaaagcc tgctctcaac cagtccagat tccaacaatg
     1801 gggaagacag agaaaaaatt gttgcggaag aaattgaaga tagaagcaat accaatatga
     1861 cgattacaaa gacgattaac cgtttaggta cagtcttctt cctatttgtc ttcaatattg
     1921 ggatttccat atttgtattc ctattaacag gaatcatgat tttctcgcag gtacttttta
     1981 tcatctatgc tatgtttctg cctgtgagct ttattttaag catgattcca tcatttgatg
     2041 gtatgtcaaa acgagccata acaaagctct ttaataccat tttgacacga gctggaatca
     2101 cattgattat tacgacagca tttagtattt caaccatgct ctatacctta tcggctggtt
     2161 atccgttctt tttgattgct tttctacaga ttgtgacctt tgcaggaatc tactttaagc
     2221 tgggcgattt aatgagtatg ttttctctac agagtaacga ttctcaaagt gtgggaagtc
     2281 gtatgatgag aaaacctcgt atgcttatgc acgctcacat gcaccgtcta cagcggaaac
     2341 ttggacgctc cgtgactgct ttaggggctg ggtctgccat tgccagtgct acgggtaaaa
     2401 aaggacagtc gggttcggga agttctgcga gtacacaagc agatcactct cgaccaaacg
     2461 ggcaggaaaa atcaacacta ggaaagcgta tcggtcaaac catcggtaca gtagctgata
     2521 ccaaagacag aatggtagat actgctggta atttgaaaga acaggttaag gatttgccga
     2581 ccaatgcaag atatgcagta tatcaaggaa aatctaaagt gacggataat gtccatgatt
     2641 taaccagaag tatttctcaa accaaagcgg ataaagccag tggacgcaag aaacagcagg
     2701 aacaaagacg gaaaaccatt gcggagcgtc gctctgaaat ggaacaggtc aaacagaaaa
     2761 aacagccagc ttcttccgtt catgaaagac cagccactaa gcaagaacaa tctcatgatg
     2821 gacagacttc aaggcaaaca actgtacagg cttcctatag ggaatctcaa caagccaaac
     2881 aagagcgtcc aacagttaag tccgattctt caagtttaaa aacggaacgt caaagtaata
     2941 cagttcaaga aagaacggtt caaaagccag taacttcaac tgcaccaaca gatagagctt
     3001 cacaacgttc aatcacaaaa gaacgtcagt ctactgttca aagagtacca ctacaaaata
     3061 caaaaagtag accaccaatc aaaaccgcca ccattaagaa aggcagtaag aaaccatgaa
     3121 gctgaaaact ttagtgattg gtggttcggg attattcttg atggtcttct cactgcttct
     3181 gtttgtcgcc attttatttt cagatgaaca agatggcggt ttttccaaca ttcactatgg
     3241 cggtgtggat gtttccgcag aagtgctggc tcataagcct atggtagaaa aagttgccaa
     3301 agaatatgga attgaagaat acgtcaatat acttcttgcg attatacagg tggaatcggg
     3361 tggtacagca gaagatgtta tgcagtcctc ggaatccctc ggtcttccac ctaattcatt
     3421 gagtacagaa gaatccatta agcaaggtgt gaagtatttc agtgagttat tagccagtag
     3481 cgaaaggctt ggtgtagatt tagaatcggt tatccagtcc tacaattatg gtggtggttt
     3541 cttaggatat gtggctaatc gtgggaataa atataccttt gaactggctc aaagtttctc
     3601 aaaagagtat tcgggtgacg aaaaagtgtc ttaccccaat cccatagcca tacctatcaa
     3661 tgggggctgg cgatacaatt acgggaatat gttttatgtg caactggtaa cgcagtatct
     3721 tgtcacaaca gagtttgatg atgatacagt acaagccatc atggacgaag ctctgaaatt
     3781 tgaaggctgg aagtatgtct atggcggagc ttccccgact acctcttttg attgtagcgg
     3841 actgacacaa tggacgtatg gaaaagctgg aatcaactta ccacgaacag cacaacagca
     3901 atatgatgta acccggcata tcccattatt ggaagcaaaa gccggcgatt tggttttctt
     3961 tcattctacc tataacgctg gctcttatat tactcatatc gggatttatt taggcgataa
     4021 ccgtatgttt catgcaggcg accctatcgg ttatgccgac ttaacaagct cttactggca
     4081 acagcattta gtgggagcag gacgaatcaa acaataagaa aggacgattc aatgatgaaa
     4141 ttcggaaaaa atcagaatac agaaaaacag acaccaaagg aaaagaaacc tcgtgtctat
     4201 aaggtcaatc ctcgtaaaaa ggttgtgatt gtcctatggg tacttttagg acttagtttc
     4261 agctttgcga tattcaagca ctttacagcc attgataccc ataccattca tgaaaccacc
     4321 atcatagaaa aggaatatgt cgatactcat catgtggaaa attttgtaga gaactttgcg
     4381 aaagtctact attcatggga actaaacgac aagtccattg ataatcgcat agaaaacctg
     4441 aaagcatatc tgacagagga acttcaagct ctgaatgtcg atacagttcg taaagatatt
     4501 cctgtatcgt cttctgtaaa aggatttcag atatggacgg tagaagcaga tggcgacaat
     4561 caatttgatg taacctacag tgtagaccaa ctcattacag agggggaaaa tacaaagacc
     4621 gtccactctg cttatatagt gagtgtctat gtagacagta ctggaaatat ggtcctcatt
     4681 aagaatccga ccattactag cataccaaag aaatcagact ataaaccaaa agctattgaa
     4741 agtgagggta cggttgattc cattacaacc aatgaaatca atgagttttt aacgacgttc
     4801 ttcaagctct atcctacagc gacagccagt gaactttcct actatgtgaa tgacgggata
     4861 ttaaaaccaa tcggaaaaga gtacatcttt caagaactgg taaatcctat ttacaatcgt
     4921 aaggataatc aagtcacggt atcgctgaca gtggagtata tcgaccagca gaccaaagca
     4981 acgcaggtat ctcaatttga tttggtactt gaaaagaacg ggagtaattg gaagattata
     5041 gaataacaaa tattggtaca ttattacagc tattttgtaa tcacgtactc tctttgataa
     5101 aaaattggag attcctttac aaatatgctc ttacgtgcta ttatttaagt atctatttaa
     5161 aaggagttaa taaatatgcg gcaaggtatt cttaaataaa ctgtcaattt gatagtggga
     5221 acaaataatt ggatgtcctt ttttaggagg gcttagtttt ttgcacccag tttaagaata
     5281 cctttatcat gtgattctaa agtatccgga gaatatctgt atgctttgta tgcctatggt
     5341 tatgcataaa aatcccagtg ataagagtat ttatcactgg gatttttatg cccttttggg
     5401 cttttgaatg gaggaaaatc acatgaaaat tattaatatt ggagttttag ctcatgttga
     5461 tgc
//
LOCUS       DASHRQ010000111         5385 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.119, whole genome shotgun sequence.
ACCESSION   DASHRQ010000111 DASHRQ010000000
VERSION     DASHRQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.119"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..177
                     /locus_tag="U2M88_002682"
     CDS             <1..177
                     /locus_tag="U2M88_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375006.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="U2M88_002683"
     CDS             190..690
                     /locus_tag="U2M88_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEM8375007.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="U2M88_002684"
     CDS             787..2634
                     /locus_tag="U2M88_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEM8375008.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="U2M88_002685"
     CDS             2637..3533
                     /locus_tag="U2M88_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM8375009.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="U2M88_002686"
     CDS             3583..3972
                     /locus_tag="U2M88_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM8375010.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>5385
                     /locus_tag="U2M88_002687"
     CDS             3959..>5385
                     /locus_tag="U2M88_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEM8375011.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPLSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGG"
BASE COUNT         1847 a          775 c         1143 g         1620 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatttctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac ccttaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaa
//
LOCUS       DASHRQ010000112         5341 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.120, whole genome shotgun sequence.
ACCESSION   DASHRQ010000112 DASHRQ010000000
VERSION     DASHRQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.120"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(62..280)
                     /locus_tag="U2M88_002688"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="U2M88_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="U2M88_002689"
     CDS             complement(546..740)
                     /locus_tag="U2M88_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375012.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="U2M88_002690"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="U2M88_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2092..3132
                     /locus_tag="U2M88_002691"
     CDS             2092..3132
                     /locus_tag="U2M88_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEM8375013.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            3956..4288
                     /locus_tag="U2M88_002692"
     CDS             3956..4288
                     /locus_tag="U2M88_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEM8375014.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4907..5248
                     /locus_tag="U2M88_002693"
     CDS             4907..5248
                     /locus_tag="U2M88_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEM8375015.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1943 a          728 c          900 g         1770 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtggta tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gaaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     3181 aaaaattttt tattattcta attatattac gaatattaca aaataatagc tattttaata
     3241 gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt
     3301 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
     3361 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
     3421 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
     3481 ttaattgtct gctatgcttg tgatgaattg attttcgttc tgcacttttg gatgataaat
     3541 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
     3601 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
     3661 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
     3721 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcacaatgtt
     3781 ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat
     3841 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
     3901 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
     3961 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
     4021 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
     4081 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
     4141 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
     4201 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
     4261 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
     4321 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     4381 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     4441 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     4501 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     4561 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     4621 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     4681 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     4741 cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt ttttatgtat
     4801 ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg aaaaaattga
     4861 tataaaacat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     4921 tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa
     4981 aggatttgtg acgtttagaa gaggcggaaa ttgtaaagga ttagatgttt tagcattaga
     5041 cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc
     5101 tgaagatgat gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga
     5161 attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct ttgaaatcat
     5221 tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc
     5281 ttagaaggag tcacgatgat taataaaaag gtagtattac cttaggcatt gagtaaggaa
     5341 t
//
LOCUS       DASHRQ010000113         5186 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.121, whole genome shotgun sequence.
ACCESSION   DASHRQ010000113 DASHRQ010000000
VERSION     DASHRQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.121"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..780
                     /locus_tag="U2M88_002694"
     CDS             <1..780
                     /locus_tag="U2M88_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM8375016.1"
                     /translation="ISSRDSEKFPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNS
                     IADHIESFVDYDFAHLLFSDGSVTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMEL
                     LSVAMLIVISTFALDFIHSDRSIFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAG
                     VYFVTQNADDLLDEKMKNNIGLKFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELE
                     NGQCLVSDIYGRVGVMQFHPIFEELLHAFDTRPPVRNEVEE"
     gene            777..2804
                     /locus_tag="U2M88_002695"
     CDS             777..2804
                     /locus_tag="U2M88_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375017.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            2801..3823
                     /locus_tag="U2M88_002696"
     CDS             2801..3823
                     /locus_tag="U2M88_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEM8375018.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDGETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            3840..4769
                     /locus_tag="U2M88_002697"
     CDS             3840..4769
                     /locus_tag="U2M88_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM8375019.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            5014..5130
                     /locus_tag="U2M88_002698"
     CDS             5014..5130
                     /locus_tag="U2M88_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEM8375020.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         1700 a          917 c         1064 g         1505 t
ORIGIN      
        1 atttccagcc gagatagcga gaagtttcct gtcctgcgta aagctattcg gagtgtaact
       61 caaagcaaac aacgagggtt gttattggtg attgatgaat tgcgaaaaga agatacgccc
      121 atcagcaata gtattgctga ccatatcgaa agctttgtcg attatgattt tgcccatctg
      181 ttattcagtg atggcagtgt cacccaatcc attagcttgg aaaaacaact caatattatt
      241 caagtggcag atttggtctt acccgattcc aatagctctt ttgaagaata cactaccatg
      301 gaattattaa gtgtggcaat gctgattgtc attagtacct ttgctttaga ctttatccac
      361 tctgaccgtt ccatttttaa aattgttgac cttgatgagg cttggagttt tttacaagtg
      421 gcacaaggaa aagctctatc tatgcgattg gttcgagcag gacgtgccat gaatgcaggg
      481 gtctatttcg ttactcaaaa cgcggacgat ttactagatg aaaagatgaa gaataatatt
      541 ggcttgaaat ttgcctttcg ttccactgac ctagtggaaa tcaaaaagac cttagaattt
      601 tttggtgtgg acaaggaaga tgaaaacaat cagaaacgct tacgtgaatt agaaaatgga
      661 caatgtttag tttcagacat ctatggtcgt gtaggggtta tgcagtttca cccgattttt
      721 gaagagttgc ttcatgcctt tgataccaga ccgcctgtgc gaaatgaggt ggaagaatga
      781 cacaaaaaca aaagaaattt cttcactata gttggataac gctcctttgt gtgggcgtta
      841 ttttactttt attaggcacg ctgggcaatg cagtacaagc gacaggctta gttgatgaaa
      901 ccattgatac aagcaatgaa tactccaagt atggtttgaa tcaatatcag cttgattatt
      961 atgtggacaa tagctggggc tggcttccat ggaattggag tgatggcata ggacaatcgg
     1021 tgatgtatgg attgtatgcc attactaatt ttatttggac aatttctctt tatctatcga
     1081 acgccacagg ttatttggtg aaagaagcct atgccttaga tttcattagt caaacatccc
     1141 aagcaattgg cgaaaacatg cagacgattg caggcattac accgaatggc ttttccagtt
     1201 cagggtttta tgtcggtttc ctccttcttt ttattctcat tgttggaatc tatgttgcct
     1261 atacaggatt ggttaagcga gaaacgacga aagtgatacg agctgtttta aactttgtcg
     1321 tggtgtttat tctatcggct tcctttattg cgtatgcacc cgattatatt gcgaaaatca
     1381 atgatttttc caaagatgtc agtaccgcca gtctgaatat tgggacaaaa attgttcttc
     1441 ctaacacgga tagtcaaggg aaagacagtg tggacatgat acgtgacagc ttattctctg
     1501 ttcaagtaaa gcaaccatgg ctattgcttc aatatggctc cactgatgta gaagcgttag
     1561 gggaagaacg tgtggaaagt ttattgtcta ccagtccaga tacaaacaat ggtgaagata
     1621 gagaaaatgt agtgattgaa gaaattgaag atcatgacaa tacctattta accctaccga
     1681 agacgatttc cagattagga acagtcttct ttttgtttgt attcaatatt ggtatctcga
     1741 tttttgtttt ccttctaaca ggaatcatga ttttttcgca agtgctattt attattttcg
     1801 ctatgttttt gccaatcagt ttcctattaa gtatgttgcc gagttttgat ggcatgggca
     1861 ggcatgccat taccaagtta tttaatgtga ttatgacaag ggctggtatt accttgatta
     1921 ttaccgttgc ctttagtatt tccactatgc tttattcgct gtcagcaagt tctccctttt
     1981 tcatgattat gtttttacaa attgtgacct ttgcagggat ttattttaaa ctgggcgatt
     2041 taatgagcct attcacttta caaagcaacg actctcaaaa catgggcaga caagtgtttc
     2101 gcagacctcg tcaaatgatg aatcgacaaa ccagaagatt tcaacgcagt atgagtaaga
     2161 tttttcgtgg aaaagcaggg tcaacgaagc aaaagacaat gacaacaaag cagaatcaaa
     2221 ccaataaagc taaccatact cgtcaaaatg aacggactac accaaagaaa gaaacagcac
     2281 tttccaatgt gaaaaagcaa caaggcggta tcgggcaacg attaggtgaa aagacagccc
     2341 atgtattgga cacgaaaaat cgtctagtca ataaaagaaa gcaggcaaaa caacagatac
     2401 aggcgacacc aacccatgtc aaatatgccc ttcatcaagg aaaagagaat gtcactcata
     2461 atgtaacaga ctttaaacaa tctatggcag ataccaaagc tcaacgcaaa caacagcgta
     2521 tgaaccagca ggagcaaaaa cgaaagaaca ttgctcaaaa gcgtttggag ctggataaag
     2581 caaaagaaaa aagacgaaaa gaaaacccat catctcctgt tcagaataac cagcagcatg
     2641 ttcgcaagcg tcagcaaacc gtcacgcctg ttttgtcaca aaacactaaa cgtgaaaatc
     2701 atcagcgacc attgaaaaaa gagcaaaaac aaaagacacc accgaagcaa ttcaatcaga
     2761 ggaagctaaa taaaagaacg gataagcgag gacaacaaac atgaagctaa aaataacagt
     2821 gatttgtagt gtggtcttct ctctcttctt ttttctctta ttattcctag tcattttctt
     2881 tgcagatgat acggacagtg gcgaaaacaa caaggattct tctattccac aaggcggtgt
     2941 gaccgtttca ccagaagtgt tggcacaccg tccgttgatt gaaaaatatg gtaaggaata
     3001 tgggattgaa gactatgttt cttatatctt agccattatg caagtggaat cgggcggtac
     3061 agcagaagat gttatgcaaa gttccgaatc tttgggttta ccgcccaaca gtttgagtac
     3121 cgaagagtcc atcaaacaag gggttaaata tttcagtgaa ttattaacca gtgcagaaca
     3181 acaaggagtg gatatagatt ctgttatcca atcttataat tatggcggtg gctttttaaa
     3241 ttatgtgagg agtcatggga aaaaatatac ctatgaatta gccgaacagt tttctaaaga
     3301 gaagtcgggt ggtcaaaaag cagactatcc caatcctata gccatacctg tgaatggtgg
     3361 ttggcgatat aactatggaa atcagtttta tgttcagttg gtttcacaat acctaaccga
     3421 cacttcccca acagaatttg atggtgaaac cgtgcaagtc attatggacg aagccttgaa
     3481 atacgaaggg tttccctatg tctttggagg ggcgtcgcct accacctctt ttgattgtag
     3541 tggcttaata caatgggtgt atgacaaagc tggcatttct ctccctcgtg tcgcccaaga
     3601 tcagtatgac gctacgcaag aaatctctat ggaagaagcc caagcaggcg accttatctt
     3661 ctttcattca acctataatg caggcacata tgtgactcat gtggcgatat atttagaggg
     3721 aaatcgcttt tatcatgcag gcgacccaat cggttatggc gacttaagca gtcgttattg
     3781 gcaagaccat ctgattgggg ctagacgtgt tattcataac taagaaagga atgaatcaaa
     3841 tgattataaa aatcgagcga aaagaaaaga aggagaaacc aaagaaatct aaaaaaccga
     3901 gcaagcaaag aaaagttccg atgattaaag ttggcacgca taagaaatta acctttgttt
     3961 tatgggtttt attgattggt agtgtcggat ttggaatcta taaaaatttt actgccattg
     4021 acactcatac tgtccgtgaa acagaaatca ttaaacagca gattgtcgat accaaccaag
     4081 tagaaagttt tgtgaaatcc tttgcgaaag actacttttc gtggcaacaa tcccaagaag
     4141 ccattgataa acgaaatgaa aaattgaccc attatttgac ggaagaattg caggtattaa
     4201 atgaagaaat gattcgtaag gatattccta ctagttctag tgtgaacgat atacaggtgt
     4261 ggcaggtttc tcaagtgaat gagaacacct ttgaggtact gttttctgtt gaacaagtta
     4321 ttaccgaaga caaagacaag gaaactatat cgtctagctt tcatgtggtt gtccatatag
     4381 atgaatcaga taatatggtt attatcaaaa atccgacaat gagtaagaaa cctcaaaaat
     4441 cggactacca acccaaacag ctagaaagtg accatacagt agatacagaa acgatggacg
     4501 aaatcactag ctttttagaa acgttcttcc agctctatcc taccgccaca gaaaaagaat
     4561 tgacttacta tgtaagtaat catgtgttac ctatgattaa caaggaatat gttttcgagg
     4621 aattggtgaa tcctatcttt accagaaaag ataaccaagt aatcgtgaat gtcgctgtga
     4681 aatatttaga tcaagaaaca aaggcaaccc aaatctcgca atttgagttg atattagaaa
     4741 aacaagataa ttggaagatt gtaaaataac aaatattggt acatgattac agatactttg
     4801 taatcatgta ctctttttga taaaaaattg gagattcctt tacaaatatg ctcttacgtg
     4861 ctattattta agtgactatt taaaaggagt taataaatat gcggcaaggt attcttaaat
     4921 aaactgtcaa tttgatagcg ggaacaaata attagatgtc cttttttagg agggcttagt
     4981 tttttgtacc cagtttaaga atacctttat catgtgattc taaagtatcc agagaatatc
     5041 tgtatgcttt gtatacctat ggttatgcat aaaaatccca gtgataaaag tatttatcac
     5101 tgggattttt atgccctttt gggtttttga atggaggaaa atcacatgaa aattattaat
     5161 attggagttt tagctcatgt tgatgc
//
LOCUS       DASHRQ010000114         5041 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.122, whole genome shotgun sequence.
ACCESSION   DASHRQ010000114 DASHRQ010000000
VERSION     DASHRQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.122"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            76..1380
                     /locus_tag="U2M88_002699"
     CDS             76..1380
                     /locus_tag="U2M88_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEM8375021.1"
                     /translation="MLNDINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLS
                     QNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPR
                     FTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILLI
                     GFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLF
                     NLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIH
                     LMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVI
                     ELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQ
                     QSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1472..1837)
                     /locus_tag="U2M88_002700"
     CDS             complement(1472..1837)
                     /locus_tag="U2M88_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEM8375022.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1834..2199)
                     /locus_tag="U2M88_002701"
     CDS             complement(1834..2199)
                     /locus_tag="U2M88_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEM8375023.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2391..2639)
                     /locus_tag="U2M88_002702"
     CDS             complement(2391..2639)
                     /locus_tag="U2M88_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375024.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2638..2766
                     /locus_tag="U2M88_002703"
     CDS             2638..2766
                     /locus_tag="U2M88_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEM8375025.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2823..4517
                     /locus_tag="U2M88_002704"
     CDS             2823..4517
                     /locus_tag="U2M88_002704"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEM8375026.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4521..4640
                     /locus_tag="U2M88_002705"
                     /pseudo
     CDS             4521..4640
                     /locus_tag="U2M88_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4617..4790
                     /locus_tag="U2M88_002706"
     CDS             4617..4790
                     /locus_tag="U2M88_002706"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375027.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKVV"
BASE COUNT         1651 a          978 c          881 g         1531 t
ORIGIN      
        1 tgtgggcaat tctatgtaaa taaagaagtc aatctagaaa agctctccaa tctcctgcat
       61 gtttgcaaaa ccactttgct caatgatatc aataacataa aaaaagaatt tgaagaacag
      121 atcgcttata cacatcggga aaaggaccgt tacactttat atttttcaga acatatacct
      181 cgatgcaaga tcatgcagca attgtctcaa aattcgttgt ttttaaaaac ttgtctgctt
      241 tatttggaag aagatgaacc tgattatctt caattaactg aatgtgaatt catctctgtc
      301 agcaaagctt attcattaaa aaaacaagta ttagcttatt ttaatgactg cgggatcgaa
      361 atcgaccgct actcccctcg attcactgaa atggaaagac gtctgctgct tcttaatgtg
      421 tcgtatcgtt taggcggttt taattcttgg gagctacctg aatcgttttt tgaacgtgcc
      481 gatcgtttca ttgaatccgt aacggaaaat agtggcagat tttatgataa agaaaataaa
      541 gaaatcttat tgattggttt tgctatcagt ttcctacgcc agcaagtgtg tgctgtgaca
      601 atagattcaa aatttatcga agaaatcaaa aaacgtccta tttacaatta tgtcgaatca
      661 gcatgggaaa acactgattt ccaaacctat tataaaaaag aagaattcgc ctttattttg
      721 acactattca atctttgtaa ttatggtttc cattcttatc agttgattgc ggaagacttt
      781 caacagctac accaagtgtt tattgacaat actcccgaaa taaaagaact agttgctacc
      841 tttgaatctc acttcaatca agaattattc ggaaaccaac cattcgaacg tgcgctgatt
      901 catctgatgc gttcggcttg ggataactat caattattca tgccagaaaa attttatctt
      961 ctaaatgagg aacagacaaa tttatataaa gaagtccaga caattttttc cagctggtcc
     1021 agccagttac cttatgatct acggttgaat cctaattgca tgcgggcttt tgtgatcgaa
     1081 ttgagcggaa ttctccgtct gacaaaagaa catctgacta tctatatcgt cacaaattcg
     1141 gatgttcatt atctgattta tcgggaagcc ttggaagctg taactacttt cgattttcaa
     1201 gttgcaccaa caatttattc aagtatatct gatataaaga agtatgcgca gcagtcctca
     1261 aaccgggtat tatgtgaacg tacattatat acaccagatg cggtccaata tgaaaatatt
     1321 attcctattt cgatcaatac gatcgatcga gctatcattt cagctgtgca aaacaaataa
     1381 gggactgtga caaaaatcat gtcacagcct cttatttgtt gaagatgccc atttctgtaa
     1441 caatctcttg atacacatca ggctcacaac ctcacttatt ttggacatct acgggtccga
     1501 tctctttatt taaaaaataa gaaatgatgg ttcggataac taccaacgcc gctagttgga
     1561 aaatatcctg caaggtaggt ttgataatcg attcgatgat cccagctgaa acaagaattt
     1621 ccaaacttag aagtatatag cctcccaaca tctttttaat agcattgttc tcgctgactc
     1681 ggtcgctttg cacttttgta tggacaaata aatatttcaa aaaatccttg attgatagac
     1741 aaacacccca aaccaataaa cagatagcaa agatatcaag tcccagtaca agtttttcaa
     1801 aatatggaag aagaaactgc atcaactctg tcgtcatcct acctctcctc ttctttgttt
     1861 ttctttgtct tctatttcct gattcaagaa ataagaaatg actgttcgaa tgataataat
     1921 aagtgcaagc ttcaaaatat ctgtccaagt cggcttgata actgactcga tgatatcagc
     1981 aacgactagt acttccaagc ttaacaaaat ataacttgct agcagttttc ttgtatctgc
     2041 attacgagta ttacgttctg catagttttt cttgacatga gaccaatcaa aaagtttgaa
     2101 tagaacaaga acgatcccga caattaagat accgatagaa caaatattta aaatagcaat
     2161 gatccattct agaaattcaa ttaaatttga gcgaaacata tacgctacct actttctttt
     2221 aatttgttcc tattctttta ttgtaaaaga aagttacctt gttcgtctat ttaaaagttg
     2281 ttctaatgat aacggttcgc ttaaattttt ctttttcact ctcccctttt tctccctcca
     2341 tccataaatc gtggaagtat ccaaaacaac gtagcaagtt aaaataaggc ttagtccgtc
     2401 atcagctttt caaagaagca ctgtctcggt gctttttttg tacccaaatt tctaaaaata
     2461 gaaccataca cctcctcaaa tcttttcttc ttacctaccc tatttttatt tacaggaaaa
     2521 tcagacactt cttctttttt gtcaaaattt cctttttctt ctcgtcctat catttggcat
     2581 tctaataaaa acgaactatc cttggatata gaaagagaaa acaaggaggc gtttttcatg
     2641 agtaaaaaat taatcaaagt cggcatcggt ttaggtttgc ttgcccttgg cgctgcatat
     2701 ctaggaaaaa agacaggact attcgaagat gacagtcatt tatatgatga attcgaatca
     2761 atctagcatc ctatttagtt gaatcactac ggacatactt ggaagacatt ggaggaatca
     2821 caatgagaaa aagtaaagca ataatgattg gtgccggtat tgccaatatg gcagcggctg
     2881 tttatttgat tcaagaaggc aaatggaaag gggatcagat caccttttat tcattggatg
     2941 atcacggttc aaatgatggt gcaccgaccg acactgtagt ggatgaatat tggaataaaa
     3001 accatccatt agaaaaccaa aaaggctata ttgctcgagg cggtcgcatg cttaattacc
     3061 gtacctatgt agatttgatg gatctgttaa gtcgaatacc ttctgttact gagccaggga
     3121 tgactgctga agaagatacg agagattttg atgcaaaaca ccgaaccttt gacaaagcac
     3181 gtttgatgga aggtggcaaa ggaatcattg atggcggaaa actgggattc aataataaag
     3241 accgtattct tctgactaaa ctgattgcta tgcctgattc agaagaagaa aaactagaca
     3301 atatcactat cgaagaatat ttcaaagatg atccacactt ttttgaaaca aacttctggt
     3361 ttatgtggga aacaacattt gctttcagaa cacgtagttc tgctcaagaa ttgcgtcgct
     3421 atatgcatca aatgatttat gaattcactc aaatcgaaca tctagtgggc gtcaatcgaa
     3481 cacgttacaa tcaatacgaa agtattatgc ttcctttgat cgagtacttg aaagaacaag
     3541 gatgcaaaat cattttgaac cgccgtgtca ctgactggga attcaaagaa acaccgatgc
     3601 aagatgaaat cactgtgact ggtttgaaaa tgaccaatac actgactcaa gaagaagaac
     3661 acgttgtggt cgatgaagat acagcagtta ttttcactaa tgggtccatt acagattcag
     3721 ctactttagg agattacgaa acaccagctg tagaaaatat ggattatggt gcagcttcta
     3781 gtctctggaa aaaagccagt gaacgtttct acaacttggg aaatcctgac aaattctttg
     3841 ctgatcgaga tgcaagcgaa tgggtcagct ttaccctgac gacgaaaaac catttgttgt
     3901 tgaatgaaat cacccgtatc acgactcaag aaccaggaaa tgccttgaat tcatttattt
     3961 caaccacacc gatcacgccg ttaggccaaa aagatgtcaa tatgtctatc gtcgtgcatc
     4021 atcaaccgca ttttacgtct cagaaaccaa atgaaacagt tatttggggt tatttccttt
     4081 atccaagacg acgtggcgaa tttgtggata aacagtatat taaaatgaat ggaaaagaaa
     4141 tgctagaaga actgattgga caactttcaa aagtagatcc tggaccagtg aatattcgtg
     4201 aaaaagaaac agaaattttc gacagcgtaa taaataatat ccctgtctat atgccttatg
     4261 cttccgcgtt gttcaataat cgtgccaaaa gcgatcgacc aaaagtcatt cctaaacatt
     4321 cgaccaatct ggcctttaca ggagaatttg tcgagcagcc ttatcagatg atctttactg
     4381 aacaaagtgc tgtacgttct ggagaaatcg ctgctttcca ttttgcgggt atccctatgt
     4441 cacgtcttgt caaaacaccg cgttacgaca aagatattcc tactctagct cgtgccgcaa
     4501 aaaaaatgtt tgaataatca agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa
     4561 caaccaggct aaaacagctt cttatgtttt tgatttggtt ggacttatta ccgcagatgc
     4621 aagcttttga acaccgttta ccaagaggtt gtgaaaaagc tgccctgcat caagtaataa
     4681 gagcagccaa gctaaaacag cttcttatgt ttttgacttg gttggactta ttaccgcaga
     4741 tgcaagcttt tgaacaccgt ttaccaagag gttatgaaaa agtcgtttag tttcgagtat
     4801 gaagaaaaat tttgtgaaaa tggttcctca cgtttttgca taaatttgac ttaataccga
     4861 gaaactgatc ccttctgtca taacctcttt ttatttatgt ctgtttcttt ttgaaaacaa
     4921 ccattttttc aacgtttact ctcttgccaa tccaacatca tctaacagat acaactattt
     4981 caatgattac cattacacta aaaaatcgga aactcacctc tccgaacaca aatacataag
     5041 a
//
LOCUS       DASHRQ010000115         5032 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.123, whole genome shotgun sequence.
ACCESSION   DASHRQ010000115 DASHRQ010000000
VERSION     DASHRQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.123"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(107..2257)
                     /locus_tag="U2M88_002707"
                     /pseudo
     CDS             complement(107..2257)
                     /locus_tag="U2M88_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2403..2978
                     /locus_tag="U2M88_002708"
     CDS             2403..2978
                     /locus_tag="U2M88_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM8375028.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3192..3887
                     /gene="vanR"
                     /locus_tag="U2M88_002709"
     CDS             3192..3887
                     /gene="vanR"
                     /locus_tag="U2M88_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEM8375029.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3865..5019
                     /locus_tag="U2M88_002710"
     CDS             3865..5019
                     /locus_tag="U2M88_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEM8375030.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1581 a          948 c          971 g         1532 t
ORIGIN      
        1 cgaaataaac gctaaaaccg ctcagaacgc aaaatatgcc attttagagc tattgtagaa
       61 aatatagttt gaaatgcaat tttcttcttt cagcttttta tttcccactt gtaaatcggt
      121 agatggtggc tccttttcca gttccataat gtggatttgc atctgcatgt agtgatgaaa
      181 cacctagctg cattctcata ccatctgacg aagatgttgt accgtcgccc caatagaaag
      241 gcaattgtaa tttatgatga aagtttacta atatggcttg ggctttattc atggcatctt
      301 catacatgcg ccattgagat acattggcta gttgcttata tgtaagtccg ggtgtggctt
      361 cggccatctt gctcaagcca atattcattc ccattcctaa aagggcagcc atgataatga
      421 ttgtttcttc cttatctggt tttcgattat tggaagcatg agtgaattgc tcatgaaatc
      481 ctgttatatg ggccacatcc atgagtaaat cagttaattt tattcttggt agcatctgat
      541 aaaggcttgc actaaatttt tttgcttctt ctggaacatc tttttctaag cgtgcaagtg
      601 atagctttcc tttttcaaga gaaaccccat ctaacttatt ggaattggca gctaaccact
      661 ttaacctttc attaaagctg ctggttctct ccgttatata atcttcgaat gataaactaa
      721 ctgataatct cgtattcccc ttcgattgat tccatgtatc ttccgaaaac aaatattcct
      781 caaaatccct atattgtctg ctgccaacaa tggaaacatc tcctgcccga acatgctccc
      841 gaagttctgt taaaacagcc atttcatagt aatgacgatt aattgttgta ccatcatcct
      901 cgtataaatg tcttttccat cgttttgaaa taaaatccac aggtgagtca tcaggcactt
      961 ttcgctttcc agattcgttc attcctcgga taatctcaac agcttgtaaa agtggctcat
     1021 ttgcctttgt agaatgaaat tccaatactc ttaatagcgt tggcgtatat tttcttagtg
     1081 aataaaaccg tttttgcagt aagtctaaat aatcatagtc ggcaggacgt gcaagttcct
     1141 gagcctcttc tactgaagag acaaaggtat tccattcaat aaccgattct aaaaccttaa
     1201 aaacgtctaa tttttcctct cttgctttaa ttaatgcttg tccgatgttc gtaaagtgta
     1261 taactttctc atttagcttt ttaccgtttt gtttctggat ttcctcttga gccttacgac
     1321 cttttgataa caaactaagt atttgcctat catgaatttc aaacgcttta tccgttagct
     1381 cctgagtaag ttgtaataaa tagatggtta atatcgaata acgtttattt tcttgaaagt
     1441 cacggaatgc atacggctcg tatcttgagc ctaagcgaga cagctgcaac aggcggttac
     1501 ggtgcaaatg actaatttgc actgtttcta aatccattcc tcgtatgtat tcgagtcgtt
     1561 ctattatttt tagaaaagtt tcgggtgaag gatgacccgg tggctctttt aaccaaccca
     1621 atatcgtttt attggattcg gatggatgct gcgaggtaat aatcccttca agcttttctt
     1681 tttgctcatt tgttagagat ttactaaccg tattaaatag cttcttttca gccattgccc
     1741 ttgcttccca caccattctt tcaagtgtag tgatagcagg cagtataatt ttgttttttc
     1801 ttagaaaatc tatgcattca tgcagtagat gaatggcatc accattttcc aaagctaatt
     1861 gatgaaggta cttaaatgtc attcgatatt cactcagggt aaaagttaca aagtcgtatt
     1921 cacttcgaat ttctttcaaa tgatcccaaa gtgtattttc cctttgagga taatgatcaa
     1981 gcgaggatgg actaacacca atctgtttcg atatatattg tatgaccgaa tctgggatgc
     2041 ttttgatatg agtgtatggc caaccgggat accgaagaac agctaattga acagcaaatc
     2101 ctaaacggtt ttcttccctc cttcgcttat taactatttc taaatcccgt ttggaaaaag
     2161 tgaagtaggt ccccagtatc cattcatctt cagggatttg cataaaagcc tgtctctgtt
     2221 ccggtgtaag caattctcta cctctcgcaa ttttcattca gtatcattcc atttctgtat
     2281 tttcaattta ttagttcaat tatatatcaa tagagtgtac tctattgata caaatgtagt
     2341 agactgataa aatcatagtt aagagcgtct cataagactt gtctcaaaaa tgaggtgata
     2401 ttttgcggaa aatcggttat attcgtgtca gttcgactaa ccagaatcct tcaagacaat
     2461 ttcagcagtt gaacgagatc ggaatggata ttatatatga agagaaagtt tcaggagcaa
     2521 caaaggatcg cgagcaactt caaaaagtgt tagacgattt acaggaagat gacatcattt
     2581 atgttacaga cttaactcga atcactcgta gtacacaaga tctatttgaa ttaatcgata
     2641 acatacgaga taaaaaggca agtttaaaat cactaaaaga tacatggctt gatttatcag
     2701 aagataatcc atacagccaa ttcttaatta ctgtaatggc tggtgttaac caattagagc
     2761 gagatcttat tcggatgaga caacgtgaag ggattgaatt ggctaagaaa gaaggaaagt
     2821 ttaaaggtcg attaaagaag tatcataaaa atcacgcagg aatgaattat gcggtaaagc
     2881 tatataaaga aggaaatatg actgtaaatc aaatttgtga aattactaat gtatctaggg
     2941 cttcattata caggaaatta tcagaagtga ataattagcc attctgtatt ccgctaatgg
     3001 gcaatatttt taaagaagaa aaggaaacta taaaatatta acagcctcct agcgatgccg
     3061 aaaagccctt tgataaaaaa agaatcatca tcttaagaaa ttcttagtca tttattatgt
     3121 aaatgcttat aaattcggcc ctataatctg ataaattatt aagggcaaac ttatgtgaaa
     3181 gggtgataac tatgagcgat aaaatactta ttgtggatga tgaacatgaa attgccgatt
     3241 tggttgaatt atacttaaaa aacgagaatt atacggtttt caaatactat accgccaaag
     3301 aagcattgga atgtatagac aagtctgaga ttgaccttgc catattggac atcatgcttc
     3361 ccggcacaag cggccttact atctgtcaaa aaataaggga caagcacacc tatccgatta
     3421 tcatgctgac cgggaaagat acagaggtag ataaaattac agggttaaca atcggcgcgg
     3481 atgattatat aacgaagccc tttcgcccac tggagttaat tgctcgggta aaggcccagt
     3541 tgcgccgata caaaaaattc agtggagtaa aggagcagaa cgaaaatgtt atcgtccact
     3601 ccggccttgt cattaatgtt aacacccatg agtgttatct gaacgagaag cagttatccc
     3661 ttactcccac cgagttttca atactgcgaa tcctctgtga aaacaagggg aatgtggtta
     3721 gctccgagct gctatttcat gagatatggg gcgacgaata tttcagcaag agcaacaaca
     3781 ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa cgacaccatt gataatccga
     3841 aatatataaa aacggtatgg ggggttggtt ataaaattga aaaataaaaa aaacgactat
     3901 tccaaactag aacgaaaact ttacatgtat atcgttgcaa ttgttgtggt agcaattgta
     3961 ttcgtgttgt atattcgttc aatgatccga gggaaacttg gggattggat cttaagtatt
     4021 ttggaaaaca aatatgactt aaatcacctg gacgcgatga aattatatca atattccata
     4081 cggaacaata tagatatctt tatttatgtg gcgattgtca ttagtattct tattctatgt
     4141 cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga taaataccgg cattgatgta
     4201 cttattcaga acgaagataa acaaattgag ctttctgcgg aaatggatgt tatggaacaa
     4261 aagctcaaca cattaaaacg gactctggaa aagcgagagc aggatgcaaa gctggccgaa
     4321 caaagaaaaa atgacgttgt tatgtacttg gcgcacgata ttaaaacgcc ccttacatcc
     4381 attatcggtt atttgagcct gcttgacgag gctccagaca tgccggtaga tcaaaaggca
     4441 aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg aacagctaat cgacgagttt
     4501 tttgagatta cacggtataa cctacaaacg ataacgctaa caaaaacgca catagaccta
     4561 tactatatgc tggtgcagat gaccgatgaa ttttatcctc agctttccgc acatggaaaa
     4621 caggcggtta ttcacgcccc cgaggatctg accgtgtccg gcgaccctga taaactcgcg
     4681 agagtcttta acaacatttt gaaaaacgcc gctgcataca gtgaggataa cagcatcatt
     4741 gacattaccg cgggcctctc cggggatgtg gtgtcaatcg aattcaagaa cactggaagc
     4801 atcccaaaag ataagctagc tgccatattt gaaaagttct ataggctgga caatgctcgt
     4861 tcttccgata cgggtggcgc gggacttgga ttggcgattg caaaagaaat tattgttcag
     4921 catggagggc agatttacgc ggaaagcaat gataactata cgacgtttag ggtagagctt
     4981 ccagcgatgc cagacttggt tgataaaagg aggtcctaag agatgtatat aa
//
LOCUS       DASHRQ010000116         4968 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.125, whole genome shotgun sequence.
ACCESSION   DASHRQ010000116 DASHRQ010000000
VERSION     DASHRQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.125"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..231
                     /locus_tag="U2M88_002711"
     CDS             <1..231
                     /locus_tag="U2M88_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375031.1"
                     /translation="KKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVDAMNVFVRA
                     ANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            1559..2527
                     /locus_tag="U2M88_002712"
     CDS             1559..2527
                     /locus_tag="U2M88_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEM8375032.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            2544..3068
                     /locus_tag="U2M88_002713"
     CDS             2544..3068
                     /locus_tag="U2M88_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEM8375033.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTETHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKTEKKWWQFWQ"
     gene            3444..3725
                     /locus_tag="U2M88_002714"
     CDS             3444..3725
                     /locus_tag="U2M88_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375034.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            3727..4143
                     /locus_tag="U2M88_002715"
     CDS             3727..4143
                     /locus_tag="U2M88_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375035.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            4765..>4968
                     /locus_tag="U2M88_002716"
     CDS             4765..>4968
                     /locus_tag="U2M88_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375036.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDL"
BASE COUNT         1864 a          840 c          824 g         1440 t
ORIGIN      
        1 aaaaaaaatt ataataagct aaagactcct gctcaaattg catcttttat tcctattggt
       61 ggagttggaa catgggttat tacaaatgct tttagtagtg ctgttgatgc aatgaatgtc
      121 tttgtaagag ctgcaaatca aggaaaaggt gtacaattaa catataatgc tcactttagc
      181 aatacaacat catatcaata caatgattac gctcgttatg taataaaata atcagaaagt
      241 gaaactatct catgaataaa aagattttag ctgtaattat ttcaataata gttttaatca
      301 ctatgttctt catatttagg ctaattttca atataagctt acaaaaaagt attctttatc
      361 taattcctat agcatttatc ttagcaattt ttagaagtat ttacggaaat aaaaactcta
      421 aatagctaaa aataaatcat actaaatttt acaaaaagtg gctagcattt gctagccttt
      481 tttgtatggt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
      541 aagcggttat ttgcaccaaa tacggaggta tttggctagc aaatatagag cttgccaaac
      601 actttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
      661 ccgtaggacg tactgaaagg gtgggattta tgagcgaact aatgcaaaat ttggctagca
      721 aaaaaagtta aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
      781 aatccgccat gacaaagctg aagcagttta tttcagcttt gtcatggcgt gtgcctagac
      841 ttttaaaaaa gtcggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaaga
      901 cgtttgaaac gtctaagtat ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa
      961 ttttagaggg cattcagaca actttagggt tgttagggct tgccctgacc gcctgtaagg
     1021 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1081 gctcatagct tctcaaagag ttccttatga acatttttag caaaggagcc ggaagcgact
     1141 gcgcagtata atgtgagagc agagcgatca caccttttta agcgactgta gggagtaaga
     1201 ttcccagttg agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     1261 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     1321 gtcggctata tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     1381 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     1441 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacagg
     1501 cattttgtat ttcataacgt gtatctcttg tgcgagtaag aaaggagcta ttttaaatat
     1561 gtctaatgaa atcgtaaagt atcatcatga attaaataca attccgttgc gaaaatttac
     1621 accagtcgaa atgaatttgt tcttttctat cgtatcaaga atgcgtgatg taggtgataa
     1681 aaaagtacaa tttacattcg agcaattaaa agatttaagc aattataaag caactgcaaa
     1741 tgttcgattc atagatgatt tagaaacaac gtatgacaaa ttaatggatt tacgttttgg
     1801 tagaagaagt gctgacggtt tgcaacgtga acgttttgtt ttatttaatc aatttaaaat
     1861 cgacggaaaa tctgatattc cttttgctga aatacaagtt catgaaaaag ccttaccctt
     1921 acttaataat cttgaagaat gggtgcgcta ttctctccaa caattcaatg aattagaaag
     1981 cagttattca aaaacaatgt ttcgattact aaaacaatac agaaccaaag gtttcgctta
     2041 tttttcaaaa gaagactttc atgaattact tgatattcct aaaagttaca accaaggtac
     2101 gattgatcaa cgtgtgttca atccaatacg acaagaacta accgcaatct ttaaaggttt
     2161 aacaatcaaa aagaaatacg gaaaaggacg gggaaagcct gtcattggct atcaatttac
     2221 atttaaacct gagataaaaa aatcagacga ctttaacaaa catgccccaa caaaagaaga
     2281 acaaaacaaa gcattagaac tagctaacca acaagttcaa tctgaaaaag cacctacttc
     2341 attacaagaa aaaatcaaag aacaaaagcg aaaattggaa cttttacaac agttagaaaa
     2401 gttagaaaga gaacaaaata cccaaaataa agaaaataat gcacaaaaat cagagataaa
     2461 ttccgatatt ccttcagaac taaaaaaaga cttattagac aatcttcaaa acttattcaa
     2521 agattgaggg gtttttagat tttatgacag aaaaaacaat caaagaactt gctgaagaat
     2581 taggagttag taaaacagca attaacaaaa aagtttctga cgaaaatcga aaactttggt
     2641 tttcgaaaat cggaaacaag tttgtgatta atgaaaccgg acaaaaggct ataaaatcaa
     2701 tgttttttaa cgaaaacgaa aactcaaatc aacaactagt tggcgaaaaa acgaaaaccg
     2761 aaacccacaa aaacgccaac caagttcccg aattagaagt cctttctctc ctagaacaac
     2821 agttaaatca aaaagatatg caactacaag aaaaagacga gcaaataaaa caaatgcaaa
     2881 aattactcga ccaccaacaa caactcaccc tgcaagccaa ccaacaaatc gagaaactgc
     2941 aggaacaact acagttgact tatacggaag aatcttcgga aaataaatca acgactcttt
     3001 ctgaagaaac agaatcgata gaaaaacaat caaagacgga gaaaaaatgg tggcaattct
     3061 ggcaataaaa ccagcaattt aaattaataa aaattgctgg ttttattctt ctttttgctt
     3121 ttgcgagcag agcaacatgg acccatgttg cgatattttg gtggcgcccc aatccccgtt
     3181 ttttcgttga atcttactcg gactgcactc gttttacagc tgatttttct gaattttgaa
     3241 agagcataag gaaacgtggt atcactttct tacgaaaggt gctagatagg ctgtgcctat
     3301 cacgtttctc agaaaaaaat ttgctatcac ttttttgcca ttgtggctag gctatgtata
     3361 gtgttttggc gaaaaatagc tatacaaata ctatatttgt atggtataca taaattatac
     3421 gctgatacga aaggaaaatt tccatggaca ttttagttcg aaatatcaat cccaaaatta
     3481 ttgcaagaat agataaacgt tgtcaggaac tcacaaccaa aacgggcaaa aaatggagtc
     3541 gaaataaata tctaaaagtt ctaatcgaaa gtgattttga ccgtcaactt atgaattaca
     3601 aaaaagatca attcgattta ttactcgaaa aatttattgc cattcaaaca gaaaatacga
     3661 acatactgaa tgactatgtt cacacaaaca accaactcat tgaaacactt atcggaggaa
     3721 tataacatga gaaaaacttc tgttcaaaaa cacgtaaccc tttatcccag tcacgttgac
     3781 gttttagatt ccatcatcga aaaaaatcca gacgtcaaca attattcaga agccattcgt
     3841 tttttatgta tgagttatga acctgatacc aatacaacca caaccagtaa ccaagcaaaa
     3901 ctcaatgcca tgggaaaaga aatttctatc atgacagaaa tcatggcaga atttgctgat
     3961 ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtgta tcaagaagtg
     4021 aaagaaaaaa ttgaaaatca actcaatcct tcccgacgaa atcaacccaa aaatcgtcaa
     4081 aaagaaacca atcaaacctt tacttctgga aattttgacc caacaaactt tctaaaaaga
     4141 taaaaaaaga aaccgatccc taaaaaaatt cctcgatttc tttttaggga tcagtcttcc
     4201 agcaagattt gcaagttggt accaacttgc gaaaaaaaag aaaaatccgc ttatgcaact
     4261 tcttcttttt gtattttggg gcgctacccc aatccccaag tatcggttat ttaatagcgc
     4321 ggaaatccaa agaataggac gtaccctgaa acaaaaaaag actgctctca cgagcagccc
     4381 aaaatgagat tatcctattc atcaacataa aaatattaca aagtgaagaa ctagagaaag
     4441 acaaaaaaag catatcaaat cctctcaaaa ataacaagtc ttaaaaaaat gacagataaa
     4501 aatgcacaaa gaattagata tagataaccg cgaaaaatca agctttagaa agttgctttt
     4561 agctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga ggggctactt
     4621 ggcatagtgt acattatgtc tactaacctt taaaataaca cgttttataa aataaaccct
     4681 cttgacaata tttaattttc tgattattct aaatatatac agaaagcgct ttcctaggat
     4741 tttatatcta aggaggaagt aacaatgaaa aataaaatta ttaaaattat tacttgtcta
     4801 tttttgttcg gtatgattgt tggagcaagt actccgttaa atctgtttcc ttttagtgct
     4861 agaggtataa aagtggaagc tgcaagttca cgatataatc ataatcatcg tggatttact
     4921 tgctataaaa tgaactatta tgttactaat aaaatgtgta gagatttg
//
LOCUS       DASHRQ010000117         4908 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.126, whole genome shotgun sequence.
ACCESSION   DASHRQ010000117 DASHRQ010000000
VERSION     DASHRQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.126"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(236..2617)
                     /locus_tag="U2M88_002717"
     CDS             complement(236..2617)
                     /locus_tag="U2M88_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEM8375037.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2607..3254)
                     /gene="recU"
                     /locus_tag="U2M88_002718"
     CDS             complement(2607..3254)
                     /gene="recU"
                     /locus_tag="U2M88_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEM8375038.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3318..3857
                     /locus_tag="U2M88_002719"
     CDS             3318..3857
                     /locus_tag="U2M88_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEM8375039.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3947..4366
                     /gene="gpsB"
                     /locus_tag="U2M88_002720"
     CDS             3947..4366
                     /gene="gpsB"
                     /locus_tag="U2M88_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEM8375040.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4388..4755
                     /gene="rnpB"
                     /locus_tag="U2M88_002721"
     ncRNA           4388..4755
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="U2M88_002721"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
BASE COUNT         1444 a          919 c         1033 g         1512 t
ORIGIN      
        1 actagtgtgt atttagccga tttaattata aacaagtaaa tggataaagt atatgtaatg
       61 ataaaagaaa aatggatttt ctaattttta agctaaatgt tagatcacac atgtatatgc
      121 tggatagtct tataaaaaat catgtaatac agcttggtac atgattttta tttgtagctg
      181 ttttttacat agacacaaaa aagaaaccac gcttgtagac gtggtttctt ttttactatc
      241 tgtttccaga agctgaggtg ctgctggatg gtgtatggtc atttgcagca tccgctccgc
      301 tagttgctgg ctcactggaa gaagcaggag gagcagatga agacggtgtc gagctttctt
      361 cggatgaaga agaaggaacc gttgttgaag acgctggctc gcttgtttct gctgtcgtgc
      421 tttcttttga ggactcagca gttgattcgc tttgactagt agagctagag ctttgagtcg
      481 ttgtctctgt tgttgagctt ccaggagtct ggacatagct ggaagatgga atagttgttg
      541 atggtgtaat tgcattcgat ctagctgtat attgatcttt gtagtacagt tcaccgccca
      601 cacgaattaa tccgcttggc atctcccagt ctgtatttgt cacgttagct gaaacatact
      661 gcataagctc gcgataaaca tcagaggcta cgtgtgacga ttctgaagtg acaggtgtca
      721 tttttttatt gtaacctgtc cacacggaaa tcgaataatt tggcgtatag cccgcaaata
      781 ggatatctgg atacacccca gaagaaatac caagttttgc atactcgtca tctgtgtaat
      841 tggatgttcc ggtcttaccg gcttgatata acccagcgat ttgcgcattc gtaccagttc
      901 cttcagtgat cgtatctttt aaaatgtctg tgatcatata agctgtttct ggagacatgg
      961 ctgtttttcc atctggctca taagtttctt ctgttccgtc ttggaagacg attttattaa
     1021 catattgagg tttgtaataa gtccctccat tggcgaaagc cgcataggct gctgccattt
     1081 ttaaggaaga agctccgtat tttgtcccat cttgttcttc cgtattacta gagatcgcat
     1141 tcgattgatg gatcgtactg tattcgatcc caagattttt taagaaagaa gccactttat
     1201 ctgaaccgac ttcattgaat aatttgactg ctggaacatt acgggataag taaagtgctt
     1261 ggcgtaaagt aatggtgccc atatattgat tatcccagtt tcctacagga gtcgaagtcc
     1321 cttcatagtt atacggtgcg tcagtaatag tttttccagt agaatatttc aaatattcaa
     1381 aagctggtcc gtaatctgtg acaggtttca tcgtagaacc aaaatcacgg gaagtattca
     1441 ctgctaagtt gttgcctaaa gttacgtctt ctgcgatgtg acgtccgcca atctgggctt
     1501 ttactttccc ggtattggtg tcgataaggg tggaggcaac ttgcatctcg tcgtctgggt
     1561 aagagacata ctgatccgtg ttgacgatat catatagttt cttttgggcg tccagatcca
     1621 aattcgtata aatctctaaa ccgtctgtgt agacattctt atctgttttt tcttgtactt
     1681 cattgatgac ttctttgaca taattatcga cgatcttagt attgtcatcg gattgagtta
     1741 gttcttgcag accatccgtt accggtacat taacagcttg atcatattct gtttgagaga
     1801 ttttttcgtt ttgaagcatc gtatacaaaa cagtatctcg tcttttcttc gcctgatctg
     1861 gataaacata aggatcatag gcggaagggg cttgaggcat cccagcaagc aatgctgttt
     1921 gcggtaaaga aagttctgat aatttcttac caaagtacat ctcagaagct gtctccatcc
     1981 cgtacaagcc gtttgacata tacactttat ttacgtagta ggtcaagatc tcttgtttcg
     2041 attttttttg ttccaatctt acagccatcc acgcttcttg ggccttgcgt ttcagagtct
     2101 gatcctcggc actggtggag aagaaggaaa gtttgatcaa ttgctgtgtc aaagtacttc
     2161 cgccttgcag tccgccagag gtaaagttag agagggcaga accgatgatc cgaatagggt
     2221 caaccccgat gtgtttataa aaacggcgat cttctaccga cacgatcgca tcttctaatg
     2281 ttttcggtaa ttcattcgca gaaatttttt cacgcttttc tgcgcctaaa tcttcgaata
     2341 gttcgccatc ttgtgtatat agtttggaag atacggtagc gtctaatttt ttatccgtca
     2401 attcgggcgc atcttttgcg taataccaga aaagtcctac acctgctaaa aagaggatac
     2461 agaaaaatga gagtattcct aaaaggatct tgataaataa tcctttttta tgtgttccag
     2521 aggactttcc agaacctttg ccagaattct tcttcacact ctttttagga gtaggctgtt
     2581 tctgtcgtct agagcttcta gtttgttcat ttgccattct tgctttctcc ttcaatagat
     2641 aatgttgata aatagaagtc aaccgcgtcc aaataaggaa tccgcggaga aataccagtt
     2701 ttgatctcta ttcctagttc ttcgatcaaa gataaaggta gtgatttgcg tcctttttcc
     2761 ttttgggcgt cccagtaagc aatcaaatga acactattca aataaaaaca gcgcttcaaa
     2821 gacgaaaacc ataacaagac aaagcagacc ccattttgtt cgaggcatgc tttcatatgt
     2881 tcgatttgat gggcatggaa gtttttaaag ggaaaagatg ttttgttctt ggtttctttt
     2941 gcttcaaagt ccaagtaaaa gcctcgatag acgccattat agtctgtagt tgaggcttgg
     3001 gtaaagtagg cttccttgat cacagcagca cttctcttag gatagtctac tttcacgatt
     3061 tggatagggg taggcttttt atggataacc gccaacccgc gggaaagata atatttatta
     3121 ctttcattga ttgcttcttc aaagcgcatc ccccgattgc cgaattcagt gatctgattt
     3181 cttttttctt gaattttttc gacgggagag gcattcttat aataaggttg gccatttgga
     3241 tagcgcaata ccatctttaa tcacactcct aggccacatt atagcaaaaa agcggataga
     3301 aagaaaagga atctgagatg gataagtcaa aagtaatcta tgtgtctggg tatcgaagtt
     3361 tcgaactcgg tgtttttaaa gaaaatgatc ctaaaatcca agttgttaaa aatgttttaa
     3421 aaaaagaact ccatcagtta gcagaagaag ggctggaatg ggtacttgtc agtgggaatt
     3481 tgggcgtcga actatgggct gtgcaaatag tagcggaatt gaaagaagaa tatccggaac
     3541 tatctcttgg tattttgtat cctttttctg attttggtaa taactggaat gaagggaacc
     3601 aagaaaaaaa acaattagca gaacaattag cagattatgt cgaagctgtt agccatcaac
     3661 cctaccaatc acctagtcag ctaaagaatc acactcgttt tatacttgag catacagtgg
     3721 gctgtctgct gatctatgat gaagaattcc ctggaaaaac gaagtatttt tttgaagatg
     3781 cacagaatta ccaagcagat catccctacg aaattcggct gatcagtatg gacgatcttc
     3841 aaaattttag tgaataatcg atattgtgtt tgatttatca acttttttcc tatacaatga
     3901 aaaaggtaga aatgttatta cgtaaatgaa aactagaggt gtaaatatgg cgaatttggt
     3961 ttatagtcct aaagacattt tacaacaaga attcaaaaca aaaatgcgcg gctatgatcc
     4021 cgttgaagtt gatgaattct tagacaatat catcaaagat tatgaaactt acagcaaaga
     4081 actccttgct ttacaagaag aaaacgatcg attgagtgca aaagtagctc aattatctaa
     4141 aactcaagga gcagctcaaa cacgcgtgca acaaacagag gcaccaaaga gcgcagcagt
     4201 gaccaatttt gatatcttaa aacgcttgtc aaatcttgaa cgtgaagtat ttggtaaaaa
     4261 gcttgatcaa caagcaagtg cagtcaaacc agcacaacct aatccaaata actacacaaa
     4321 tgcagacaca agcttagatg ataatgaaaa aacacgtcaa ttttaatttt tgattaatga
     4381 atttgcggtt ttcgggtaat cgcggtctga cttgatcagg ctgaggaaag tccatgctcg
     4441 cacaggctgt gatgcctgta gtgttcgtgc ttagcgaaac cataagctaa ggtactcttt
     4501 ggagtaacgg caggaaaaca agctaaggct tcggctatgc ttaagtatcc ttgaaagtgc
     4561 cacagtgacg aagcggctgg ggaaacccat ccggtggaac gcggtaaacc cctcgagcga
     4621 gcaacccaaa caatggtagg ggcgcttttt gtctggaaga agaacaggac aaaaaggcag
     4681 tataactgca gatagatggt taccgctttg ttttttctcc ctggaaaagc aaagtacaga
     4741 acatggctta tagaaaaccg caaacatcag ggaattaggt cgtgacaaaa gtaatgcgtt
     4801 ataagaaaca agaaaaacat ttgaaagcta tttttcaata cttcagatga atcgttctta
     4861 ttacaaaaca aacttttgta tcaccggtta ttcggaattt gagtaaaa
//
LOCUS       DASHRQ010000118         4886 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.127, whole genome shotgun sequence.
ACCESSION   DASHRQ010000118 DASHRQ010000000
VERSION     DASHRQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.127"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(142..417)
                     /locus_tag="U2M88_002722"
     CDS             complement(142..417)
                     /locus_tag="U2M88_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEM8375041.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(660..1970)
                     /gene="der"
                     /locus_tag="U2M88_002723"
     CDS             complement(660..1970)
                     /gene="der"
                     /locus_tag="U2M88_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEM8375042.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2152..3381)
                     /gene="rpsA"
                     /locus_tag="U2M88_002724"
     CDS             complement(2152..3381)
                     /gene="rpsA"
                     /locus_tag="U2M88_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEM8375043.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3516..4196)
                     /locus_tag="U2M88_002725"
     CDS             complement(3516..4196)
                     /locus_tag="U2M88_002725"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEM8375044.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4245..4880)
                     /locus_tag="U2M88_002726"
     CDS             complement(4245..4880)
                     /locus_tag="U2M88_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEM8375045.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
BASE COUNT         1319 a         1073 c          828 g         1666 t
ORIGIN      
        1 taaaaattaa ataaaataca tgtgttttct atgtatgaca tacggattcg gaatctctgt
       61 ctcttattta ttccttttct gctaggaaat aaaaagaaaa gcacttgtag ccctagagcc
      121 acaagcgctt tttcaactgt cttatttaac agcgtctttt aatgctttac ctggtttgaa
      181 tgcaggtact ttgctagctg gaatttcgat ttcttcacca gtttgtgggt tacgaccttt
      241 gcgagctgcg cgttcgcgaa cttcaaagtt accaaaaccg attaattgaa ctttttcgcc
      301 gttagctaaa gcatcttgaa tagttgaaaa tacagcatct actgctgcag ttgcatcttt
      361 tttagttaaa tcagtagctg aagcaacttt ttcgattaat tctgctttgt ttgccatgga
      421 tagattcacc tcctccacaa gagatatgat agaaatatca tttagttaaa actttctgag
      481 tggtccagaa gtttaaaaag aaatattgat taacttcaat attctgttat cacgatatca
      541 tatgaaccgc ttaatagcaa ggtttttacc aattttcaca tgcttttttt agtcatagaa
      601 acgtaacaaa cgaaactgtc tttttctttt gaaaaaaaaa cgtcgatatg gtaaaatgcc
      661 tattttctcc tacgagggat aattcggatg gacgtccctt caaaagtaaa tgcttttctg
      721 atttgatttt ctaagaaacg ttcataagaa aaatgcatca attcctcttc attgacaaaa
      781 acgacaaatg taggtggttt gacagcgact tgtgtagcat aaaaaatctt caggcgtttt
      841 cctttatctg ttggtgttgg attgattgct acagcatcca taatgatatc attcaacaca
      901 gccgaagaga tacgaagatt ctggttcata cttacttgtt cgatcatttc aggcagacgt
      961 tccaaccgtt gtttagtcac tgcagaaaca aaaacgattg gagcgtaatc aagataccgg
     1021 aattcatcac gaatctcttg ttcgaaatca cgcattgtat tggtttcttt ctttacaaga
     1081 tcccatttat tcacgacgat aataattcca cgaccagctt catgggcgta acccgctact
     1141 cgcttgtctt gttcccgaat cccttcttca gcattcagta ccatcaaaac gatatcagaa
     1201 cggtcaatgg cacgcattgc acgcatcacg ctgtattttt cagtattttc gtatactttt
     1261 ccgcgtttac gcataccagc tgtatctatc attaagaatt tctgaccact ttcagaaacg
     1321 aagtgggtgt cgattgcgtc tcgggtcgtt ccttcaatat ccgaaacaat tacacgttct
     1381 tctcctaaaa tcgcattgat caaagaggat ttaccaacat ttgggcgacc aatcaaacta
     1441 aacttaatcg tgtcgtcgtc ctcttcttca gtctcttcag agaaatactt cactgcttca
     1501 tctaaaatat caccaatccc tagtccatgg cttcctgaaa caggataagg atcgcctagc
     1561 cctaaggaat aaaattcata gatatcgttt ctcatttctg gattatctac tttattgact
     1621 gctaaaatca ctggtttatt acttctgtat aaaattttcg caaccatctc atctgcgtct
     1681 gtcacgcctt cgcgtccgct gactacacaa atgattacat ctgcttcttc aatcgcgatt
     1741 tctgcttgat gcttgatctg atccataaaa ggttcatcgc caagatcaat tccgcctgtg
     1801 tcgatcacgc tgaattctcg acctaaccat tcaccttttg cataaatacg atcacgagtt
     1861 actccaggag tatcttcgac aatcgagata cgctcaccag cgatgcggtt aaaaatcgta
     1921 gacttaccga cgttcgggcg gccaacgatt gcaattgttg gatttgccat attatttcct
     1981 cctcacaagt ttccaatctc acttagttta acgaatgcat tgccaacttt caagcataga
     2041 ttgtcaatta aacgaagtat ttttattcct tattgaaata ctaatcgctt ttcaaaagaa
     2101 aaactgagac aaaagcagct gctttcatct cagtcacaga tacaaaatca attatttttc
     2161 agaatcatcg gttgcttttt cttctacttc ttctttcaac gcatcaccta aaatgtctcc
     2221 catagtaaat ccagtatttt cttctggtaa ttcataagac tcaacatttt ttggttcttc
     2281 ttctgaagct ggtttttctt ctaaagcttt gatactcaat gcaatacgat gttcttctgg
     2341 atggatctct aatactttca ctttcacttc gtcaccttca tgaagcactt catgtggtgt
     2401 cgcaatatgt ttatgtgaaa tttgagagat atggactagc ccttctactc cagggaacac
     2461 ttcaacaaag gcaccgaagc ttgtcaaacg tttaactgtt ccgtctaata ctgtgccaac
     2521 tggagctttt gtatcgattt ctgaccaagg tccaggctgt gtgtctttga ttgacaagga
     2581 aacacgttct tgttcaggat tgatagacaa cactttgaca tttacatcat cattgacagc
     2641 caatacatca cttggttttg ctacatgact atgtgaaatc tctgagacat ggactagacc
     2701 gtcaattccg cctaggtcga caaatgcgcc aaaatcagta agacgtgcaa cacgtccact
     2761 gagaacatct ccctcatgga tagttgaaag aatttctttt ttcttttctt ctctttcgtt
     2821 ttccactacc gctttatgcg ataagatcaa acgattttct gatggttcga tttcgatgat
     2881 tttaaagctc aatgttttgc ctttatagtc tgaaaagtct gcaacaaaat gatcttccac
     2941 cattgatgca gggacaaaac cacgtacacc gacgtcaacg actaaaccgc ctttgacaac
     3001 atttgtaaca ggtgcttcga tgaccttgcc ctcttggaaa tctctttcga tatcttccca
     3061 aactttttta gcatctaaac ggcgttttga aagtaggtag ctgccgtttt ctttatcttt
     3121 gccgatcgta ctgataacga ctaaatctaa tacatcaccg acttttacta cttcgctgat
     3181 atcttctacc ggcaaagttg ataattcttt tgccggaacg actccttcaa ctccagcccc
     3241 ttcgattccg acaatcactt gtttgtcttc aatgactaaa acttcgccct taacaacatc
     3301 gccaactttt acttcttgta cgcttttcat tgcgtcttcc attgactggt tgttctcttg
     3361 attttgatca aattctgtca taacacttgt cctcctaacc aacattctgc cgtaagtacg
     3421 gtactgtttt tagtttaatt gattattcaa aaaaaataaa gcgatttcgt tcgttttcct
     3481 tgaaaaaaca attgtttttc attttttttc ttaatttaaa aaagaagata accttttttc
     3541 aaaacaacag cttttattgc tgcaacgact tctggaatcg atttgccagt cgtatcgata
     3601 cgaacagcat cctctgcttg cttcagcgga gagacgtccc ttgttgaatc ataatggtct
     3661 ctttcctgta tctctttcgt cagtgtttca agatcagtcg gaattccttt ttcttggttc
     3721 tctttgaaac gtctttctgc acgctcactt acgcttgcta ctaaaaagat tttcacttct
     3781 gcattcggta atacagctgt cccgatgtct cttccgtcca tgacaacgcc accgcgttcc
     3841 cctatttttc gttggacagc aactaattct tctctgactt ttccatgtgc agagacttca
     3901 gaaacagctt ttgtgacatc tggttgacgg atttctaaag tcacatcttt tgatgctatg
     3961 aatacatgct gtccatcttc ttgttgcgaa aaagagatag gatacttttt gatcagcgaa
     4021 actagccctt cttcatctga aaaagataca ttgttcgtaa tagctaaata agttactgcg
     4081 cgatacatgg caccggtatc tgtataaata taattcaatt cttttgctaa tatcttggca
     4141 acggtacttt tcccagatga agcaggcccg tcgattgcaa tactgatttg tgtcatgata
     4201 taactccttt ttctcagaaa aaaagccgca aagcgcggct attattattt aattcttaat
     4261 tcttgaccag ggtaaagcat aaagttgttt ggatctaagc cattcaattc aaacaattga
     4321 tcagtggtga tccctgcacg ctcagccact tgttttggac cttcgcctgc ttgaacagtc
     4381 gttgttgaac cggctgcagc tgatgaagaa ggtgttgttt ctgctgcatt gctgcttgaa
     4441 tcccctgctt gataagttgc ctcacttgag gaagtcggtg ttgtctctgc tgcaatactg
     4501 ctttctgacg ttacacctgt atcttgagac acagtagatt cttccgcgat tgtactagat
     4561 tctactgttg aagtctcagt tgactcctct tttgttgtac ttgtagaact ttctactact
     4621 gatgtcgaag tttcagaaga agttgctgct gttgcattgt tgcttgatcc atttctgatc
     4681 cagaaataag cacctgctgg aatagcgata cataagacca gtaaaatcaa aaatagcgaa
     4741 agaaataatg tatttccttt cttttgttgt ctctgagaac ttcttgatga aacttcatca
     4801 tctgtatcgt agattggttg ttcccatggt tcttgtgttt cgttattttc tgtagattga
     4861 tgtctatctt ttctgctcac tgtttt
//
LOCUS       DASHRQ010000119         4884 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.128, whole genome shotgun sequence.
ACCESSION   DASHRQ010000119 DASHRQ010000000
VERSION     DASHRQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.128"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(207..1169)
                     /locus_tag="U2M88_002727"
     CDS             complement(207..1169)
                     /locus_tag="U2M88_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM8375046.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="U2M88_002728"
     CDS             complement(1171..2610)
                     /locus_tag="U2M88_002728"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEM8375047.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="U2M88_002729"
     CDS             2795..4741
                     /locus_tag="U2M88_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEM8375048.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1571 a          826 c          795 g         1692 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaa
//
LOCUS       DASHRQ010000120         4807 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.129, whole genome shotgun sequence.
ACCESSION   DASHRQ010000120 DASHRQ010000000
VERSION     DASHRQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.129"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="U2M88_002730"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="U2M88_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEM8375049.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..3768)
                     /locus_tag="U2M88_002731"
     CDS             complement(3250..3768)
                     /locus_tag="U2M88_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEM8375050.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1579 a          786 c          730 g         1712 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcggta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     4381 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     4441 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     4501 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     4561 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     4621 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     4681 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     4741 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     4801 ccactta
//
LOCUS       DASHRQ010000121         4697 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.130, whole genome shotgun sequence.
ACCESSION   DASHRQ010000121 DASHRQ010000000
VERSION     DASHRQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.130"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            6..305
                     /locus_tag="U2M88_002732"
     CDS             6..305
                     /locus_tag="U2M88_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM8375051.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="U2M88_002733"
     CDS             378..1739
                     /locus_tag="U2M88_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEM8375052.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="U2M88_002734"
     CDS             1761..2021
                     /locus_tag="U2M88_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375053.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="U2M88_002735"
     CDS             2035..2787
                     /locus_tag="U2M88_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEM8375054.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="U2M88_002736"
     CDS             complement(2881..3486)
                     /locus_tag="U2M88_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM8375055.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="U2M88_002737"
     CDS             complement(3539..4513)
                     /locus_tag="U2M88_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEM8375056.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
BASE COUNT         1418 a          806 c         1001 g         1472 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaa
//
LOCUS       DASHRQ010000122         4616 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.131, whole genome shotgun sequence.
ACCESSION   DASHRQ010000122 DASHRQ010000000
VERSION     DASHRQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.131"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(15..962)
                     /locus_tag="U2M88_002738"
     CDS             complement(15..962)
                     /locus_tag="U2M88_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEM8375057.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1140..1943)
                     /locus_tag="U2M88_002739"
     CDS             complement(1140..1943)
                     /locus_tag="U2M88_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEM8375058.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2089..3003
                     /locus_tag="U2M88_002740"
     CDS             2089..3003
                     /locus_tag="U2M88_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEM8375059.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3051..3521
                     /locus_tag="U2M88_002741"
     CDS             3051..3521
                     /locus_tag="U2M88_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEM8375060.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3606..4214
                     /locus_tag="U2M88_002742"
     CDS             3606..4214
                     /locus_tag="U2M88_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEM8375061.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4348..4548
                     /locus_tag="U2M88_002743"
     CDS             4348..4548
                     /locus_tag="U2M88_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEM8375062.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         1511 a          716 c          912 g         1477 t
ORIGIN      
        1 attgttttca gctattaata tcctgcagac aattttactt gattggtttg aagctcatgg
       61 gtggcaaagt agcttttcag attggttaaa aaaatagcca ttaatttctt ttgaaaatca
      121 ggtgtcaaac ctgcaatgtg ggaagtaatc agtacattgt ccattttcca aagaggatga
      181 tcttgtggaa gaggctcttc ctcaaaaacg tctaatgctg caaaggaaag ttgctggtta
      241 tttaaagcct gcaccagatc ttgtgtctta acagaagcac cacggccaac attgatgaat
      301 acagattttg gatcaaagtg ttcaaacata tgctgatcaa agagcccagt tgtttcctcc
      361 gttagaggta agatattgac tacgaaatct acttcaggaa gaacttcctt tagttgattt
      421 aaagaaaaag tcttttccat tccttctgct ggatgaccgg atgtattgat tccatagcag
      481 ttcatgtcta atgctttgca tttttttgca atcatttttc caatatggcc agttccaatg
      541 attagtattt ttttcccttg gatagattgg taatggaccg atgaacctaa ccatttcatg
      601 tcactctgtg ccttttgtgc ttgaaaaaga ccacgagaat acccaaggat gattcctaag
      661 acatgctccg taatcgattc actgtggatg ccactaccat ttgaaagtaa aatatgtttc
      721 tttgaaaaag tctcaagggg tagctgatca acaccagctg agatagattg gacccatttc
      781 aaggtagaag aggctaatag tttttcttgg tagtctgagt tccagcccaa actgatctct
      841 acattttgta gatcctctgg ttgtaattct gttttaaaga tataaccagg cgctatagta
      901 tggatttctt cgataaattc atcacgaaat ggacgatctg cataaatgat tctttctgac
      961 atagttaatt tcccgccttt ctttactcta agtgtagcgc atacaagaag aactttgaag
     1021 cgccttgctt gttttttgag aaagaaatat ttttcaaata aaaaaggggc tgtgacaaaa
     1081 aaatgtcaca acctctaaaa gggtctattg aagtagaaat aacgagttgt ctttatttct
     1141 taattgatcg atatttttgc ccatcctttg acagcttgag caaaaataaa gattcctgca
     1201 aaagcaatga aggcgctagt gtcaaaagaa ggaagaaaga ccatcaagat tgttgcaatt
     1261 atagtaggca atgaaattga atagatcaat gtttttaaac aatcaagaaa agtagctttt
     1321 gttagtcgaa agcgtgcata gagaaaagca gcaatgttgg taaatagcaa agtgatcagc
     1381 agattcagaa aactaggata aatagaaaat ataaatgtca ggacttttat ccaaagtggt
     1441 atagatgctt cacttaaaaa agaacggagc tgcttacctg ttaagttttt taaagattca
     1501 ttggtgtagg gaagttctaa ttggttattg ttcaacaaag cagaagtcgt gcctgtatta
     1561 ggaagagcta tgataagttt gttttttaac atcccgacac taacaaaatt ccccatcaaa
     1621 tcagtagaga tgtcctgttc tgttcgtttt ccctctggat caaatgtgaa gataatggag
     1681 tttgtttggt aaatgaatcc ttctttttct tcggtttgaa gctgcccgtt ttggattgta
     1741 aaatcaggaa tgcgagtagc aatcttttgc ccatccaatt gaatgttgtt taagacttga
     1801 aaaacttgaa aagagattgg caaagccata attatactca aaactaaaag atagacaatg
     1861 gctttccaaa atgggacatt tctcgcctcc tttaggtctt tgaatcgtat aaaggaactg
     1921 ataattaatt gttttgttgt cataagtact cctcatctag tagtgtcatc acttgcaatt
     1981 ctagcgaagt atcaagtgaa atacaagtgc tttaaggaaa cagctgaatg attggtgaaa
     2041 tatcaaaaaa atgttattct tgtctaaaga ctatccaata taaataaaat ggaggaagga
     2101 atagtgccta cacagccaca gagatcatca aaaaaactat ttggagtagt ggttttgaat
     2161 gcggtgatca ctttatcaga atttattgga gggattatgt cagggagttt gtctcttgtt
     2221 tcagatgctt tccataacct tagtgataca ttggcaatta ttttttctta cggggcacaa
     2281 aaaatggccc gaaaagaagc aaataaaaaa cgtacgtatg gctaccagcg tttagaaata
     2341 ttgagtgctt tcatcaactc attcatattg atcatattat ccttgttttt aaccgttgaa
     2401 gcattcaagc ggttcacttc accagaaaag ataaatagtc atttgatgct aacagtagca
     2461 gttattggat tgctcgcgaa tttgttctca gtattattat tacgacaaga agcagatgaa
     2521 agtttgaata taaaatctag ttatttgcat ttgttaagtg atacactgtc ttcggtaagt
     2581 gtgattatcg gagcaatttt gatccgtttt ttcgatatct actggatcga tccagttatt
     2641 acactgatca tttcgatata tatattgata gaagcgataa tggtgatcaa aaaagctgcc
     2701 gctattttga tacaaagtgc acctgctatc gactatgaaa aaatggaaca agaaattgaa
     2761 gcaatcgatg gggtaaagga tgttcatcat gtccatattt ggcagtatag cgaaaagata
     2821 attattttcg acggacatat tgattttgaa gatcagctac taagcgagat tgaaaagata
     2881 tatcctcgta taatttcttt attaaaatta aaatatggta ttacacatgt gacgatccaa
     2941 gcagagacaa aggcaaagga tcaaaaaaaa ttaatttttt taaataaaga tgaaagttat
     3001 tgaaatatag agtatgataa ataagaattt ttaataaggt aggttggctc atgaagcagt
     3061 atcgacagtt aaaaggatat ttagaagcaa aaggatattg ggagatacta gtctatctct
     3121 tttttggtgg tttggctacg attgtaaatt ttgttagttt tgctctcgca cgccaatatt
     3181 ttgatttaag tatggcctta tccaattcta tttcgtggtt ttgttctgta ttgtttgctt
     3241 ttgtaacgaa caaactttgg gtattccatt caaaatcacc gaattttaca catgcactta
     3301 ttgaatgcgg taagttcttc ttctaccgta tcctttctta cggattggac atgggggcaa
     3361 tggtattgtt gatcaatgtt atgaacagca atgagtatgt ggcgaaaatc atcactcaaa
     3421 ttattgttat ccttgcgaac tatattttca gcaagttgtt tatttttaaa gaaactgagg
     3481 tttttgaaga agaagtaggt caaagtgaaa aagacgacta ggcataacaa ttgaagaatt
     3541 tccttctctt tgttactctt tgtaatgtag acattattaa gaaaacattg aggaggaatt
     3601 tttttatgac ttacacatta ccagatctac cttatgcata tgacgcattg gaaccttata
     3661 ttgacgaaga aacgatgcat ctgcatcatg ataagcatca caatacttat gtgacgaatt
     3721 taaattcagc aattgagaaa tacccagaat taggcgaaaa aacaatagaa gaattattat
     3781 ctgatatgga cgctattcca acagatatca agacagctgt acgtaacaat ggtggcggac
     3841 atgctaacca ttcatttttc tgggaaatca tggcaccaaa tgctggtggc gaacctacag
     3901 gagaaataaa agaagcgatt aatgaagctt ttggtgattt ttcttctttt aaagaagaat
     3961 tcaaaaaagc agccgctgga cgatttggtt ctggatgggc ttggcttgta atggaaaatg
     4021 gaaaattagc tattacctct actgcaaatc aagattctcc attgatggaa ggaaagacac
     4081 caattctagg tttggacgtt tgggaacatg cttattattt gaaatataaa aatgtccgac
     4141 cagattatat tgcagcattc tggaatgtta ttaattggga tgaagtaaac aaacgtttcg
     4201 aagcagcaaa ataaccattt cctccgtcat atcagtctta aaaataattt ggaaaataaa
     4261 agagcgaaag cttgctagcg ttttctaaat tgggtataat gttcttgcaa ggttacttgc
     4321 aagaactttt ttggaggata tacatttatg gaacacggaa cggttaaatg gttcaacaat
     4381 gaaaaaggat ttgggtttat tacagtagac ggcggagatg acgtatttgt tcatttctca
     4441 gcaattcaag gagacggctt caagagccta gaagaaggtc aagaagtaga attcacgatt
     4501 gttgaaggtg cacgcggacc tcaagctgct gaagtgacta aattataata atttgttgaa
     4561 taaagacacg acttcttaga gagtcgtgtc tttttgtcta tttatgaagt ttttag
//
LOCUS       DASHRQ010000123         4574 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.132, whole genome shotgun sequence.
ACCESSION   DASHRQ010000123 DASHRQ010000000
VERSION     DASHRQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.132"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..103
                     /locus_tag="U2M88_002744"
     CDS             <1..103
                     /locus_tag="U2M88_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEM8375063.1"
                     /translation="EAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(719..1759)
                     /locus_tag="U2M88_002745"
     CDS             complement(719..1759)
                     /locus_tag="U2M88_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEM8375064.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2108..2434)
                     /locus_tag="U2M88_002746"
     CDS             complement(2108..2434)
                     /locus_tag="U2M88_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375065.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2421..3224)
                     /locus_tag="U2M88_002747"
     CDS             complement(2421..3224)
                     /locus_tag="U2M88_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEM8375066.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3577..3867)
                     /locus_tag="U2M88_002748"
     CDS             complement(3577..3867)
                     /locus_tag="U2M88_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEM8375067.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4003..4182)
                     /locus_tag="U2M88_002749"
     CDS             complement(4003..4182)
                     /locus_tag="U2M88_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375068.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4179..4529)
                     /locus_tag="U2M88_002750"
     CDS             complement(4179..4529)
                     /locus_tag="U2M88_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375069.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
BASE COUNT         1392 a          795 c          654 g         1733 t
ORIGIN      
        1 ggaagccatt cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc
       61 ggtctgtact gaaatcaagg tattattggg aatcccagct taaatcatag ataccgtaag
      121 ggattttatt ctttatttaa aactttgcaa cagaaccgtc cgttgcgggt aataccgtaa
      181 cactgaaggg cacgcattaa ggcgtcatct taatgctttc ctgtaggaca ctgacggaga
      241 ttaccagtct cccgagcctt acaggggcct ttcttttttt tgtattttca attatcacaa
      301 tttatcgtcc aaaagtgcag aacgaaaatc aattcatcac aagcatatca gacaattaaa
      361 tttaaaacaa ctaaaaatat acaaaaaagt gtaattgaat attagcagca ctgcctgaaa
      421 agaagtaata tcaggctttt cagcttgggg aaataagtga attacctctc ttcatgaatt
      481 attataactc aaaaaagcac atatgcaaac actagttccc tatttttttg aatgaaaaat
      541 aactaaaata tctaacgatt atgactttaa aacctacgtt tgtgatcgtt ttcataaact
      601 tttaatctaa aaactccgga ataatagcta tttatttgta acatttgtaa taaaattaaa
      661 atcataaaaa agttctaatt taaaattgat catttttata gtgatcgctt tttatcaact
      721 actctagcca attttccgta ctaactgttg gtaattcgtt aaatgtttct ctttgttcgt
      781 ttgcaagctt tgccgcaata gctttttcga caaaccaatt ttttacataa acgaaaatta
      841 gattctgcaa attgtttact ttttcagttt tttgtttttg ataagctttc agcaaagtca
      901 tatataatcc tttgtcagca tcaattccat aagattccag ttcttcataa acaacaactg
      961 tttgctctaa ttcttcagct tttttctttg cattatgaat agtttggacg gttttttgag
     1021 cgtcttcgta agtactagaa aatgttttta tcaagtttaa tacattttct ggtacaaatg
     1081 tatttatact gtcctctttc atgatgtcga cgaagttatc tagtaaaact tgattttgta
     1141 actgatcagt ttcagtgtct attatgtgtc tattagtgtc tatagaatta gaatagaata
     1201 gattgtctgc gattttcgca attacggttg agtccttgat atgactggat tttgccgtat
     1261 ttgcgatttt cgcaattacg gtggtatcat tgatttctcg cggttttgcc gtatttgcga
     1321 ttttcgcaat tacggtggta tcagtattgt cttcctcttc catgatttta aagacgtctt
     1381 gattcgttac ttctggcttg cctaagtaca ataaattagg catggattca atcttaccgt
     1441 cacgtttatt tactcttcct ctcttctgtt tcagaagacc tgctttttct aattcctttt
     1501 tgattttaga tactttacct tcgcggcatt gtaacaattt cattaattct tcaactgtga
     1561 atatgaaaaa tatattatca tcagaatcta cccagttatt tttgactgaa taatcaaatc
     1621 gatctttaag tagcatataa gcaatttttg catcatttga tagatccatg tatttttcgt
     1681 tagtaaaaaa taccttaggc atttgataat atttttcacg ataaatttct tttgatttgt
     1741 aataattaaa atcattcata gccaatcctc tttctgtagg atagcgtttt gttcaaaact
     1801 atgttacaat gaattaaaca aatatatcta tccactatat atttttctac tgctgataag
     1861 tcgccaaact ttgttaaagc agtagtcttt ttttatcttt ttttagttta ccttaaaaaa
     1921 taaattaagg caaaccgctc tatttcaagg tttaagctca tagtaactat atcacgatat
     1981 tacaataata ttatatcacg atatcgtaat attacaatat gaagataagc gacttatcag
     2041 ctttgatact actgattttt ataaaaaaaa tatcacgata ttatgatatc gtgatattga
     2101 atgtaaatta aattttattt tgtttctttg ctagtttata tccttcaatc aagtattctt
     2161 caggtatgga ctttaatagc gcatcttcta taacgtctgg tttacttttg tctgattcag
     2221 caatagtcgt ccaatatttg agtgctgaca ttagaattgg atttatacga atgctatctg
     2281 ctgttcttac actacttcct tgatctttta tgactttact tatatttttc ttggttaatt
     2341 cgatttcttc tacttctttt gatttaaaat tctttttatt tttatttcct agtaatctac
     2401 ttactgcagc ttgactgcga ttatcttctg ccatcaaaat caccaacttc ttttaaatat
     2461 tcttctgtaa atgctttata gtcatcttca gctgttgagg gtttattaaa attgattaga
     2521 tctgatccag cagcttgcgc ttcgtcaacg gttattgtcc tacgaatata agttttgaaa
     2581 atttttgcac caatgactct agaaatttcg gttgctgcag acgtcatttc tttagaaatg
     2641 ttataacgct tattatactt agtaaataga actcctcgta cttcaagctt gtcgttcgta
     2701 tattctttta ccgtttcaat agtttcactt aactttatta gtccttttaa tgagaaatac
     2761 gcagcttcaa ctggaatgat aatttcatca gaaacagtaa atgcgttgac tgtaagcatt
     2821 cctaaacttg gtggacaatc aattataata taatcatagt tgtccttgat tggttgaagt
     2881 actcttttaa gcctgtgttc acttcctact tgagtaaatt ccctctctgc agaagaaagg
     2941 gctatgtcgg ctggcaaaac atcaattcct ttatagtcca gaatagcatc tgtaatgtca
     3001 gcctcccctt ttaatacttc ataaattgta ggttggtttg ttccatctat atcgaaacct
     3061 acattatctg tagcatttcc ctgaggatca atgtccacta aaagtacatt tttaccgtaa
     3121 acaaatttca aacatgctgc taaattaacg ctagtcgtag ttttagaaac tccacctttt
     3181 tgattggcta ctgacaatat tcttgccatt aaaatatccc ccattcatct tatattataa
     3241 tataattata tcataatatt gtgatattgc aatatcgtat atgcattata ttgtaatatt
     3301 gtgtgattat tttgttttaa agatatatat cggttcggat ataaagatta ttcatatgtt
     3361 attcgaaatt aaaataatca ctttttatta ctttaaaatt ttttattaat gataagaaat
     3421 atgttttgcg agcagcaact agaataattt aaacattact aaactgtcta tattgttact
     3481 aataattccc ttcttatagt gcattttctt agaaaaaaac aatattacga tatcatgata
     3541 ttaagatatc gtaatattat acaattgtga ttgatattat atatgttttt ttattttttt
     3601 atcatgttta atcttgaagg aatcatattc tttttctaaa gacaggagct tttcataaac
     3661 atcagacatg tttaaactag aggccaggct taaatcataa acgaatgaag caggtaatga
     3721 ttttattcta cgttgtcggg ttatccagct tgcaaccgtt ccttgtgggt aggcatataa
     3781 ctcacaaaat tgttctacag tgattcctaa attcttgata atgtaaacat tgatcgggtg
     3841 cgagtaaaca aatgtattga tagacatggt gcgtaagcct actttctttt attattgcgt
     3901 ttgcaataat agtttaacac acgcattatt ctagtatcta ctcctatttg taaaaacaaa
     3961 aaaggattca tagagataac tctatagaat ccgtaaaata ccttatatat ataacgggaa
     4021 gatataaaac ctaaaattat gataacctgc tgcagcttct acatagaaag agaagcgctc
     4081 aacaccaacc tcatttatca aacgttggcc ataaccccaa atctcttctg atagctgatc
     4141 aaaggttata tactttagct catattgctg gataatgttc attctaatcg actccattta
     4201 ttatcctggt aaaattcgat atgacgtatt tcgtcgtagt gaattttgtc ttttccaacg
     4261 tagatcccca gctcatcact acctaagatc ttgccaacaa tatcttctgg atagtatcct
     4321 tcagcgtttc gttcttcttt ttgaatagat actgatctat tttttatgac tgcagcgttc
     4381 aatacttccg caatctcttc agtcgtcatt tgttcttttg ctaaattttg actggttcgt
     4441 tgttcattgt cggcatcaat ttcttcagta tgatcagata aataaaaccc tgcccacttt
     4501 aaacgtccac gatcattgta atcaatcatt tttaattccc ccatttccgc ccgcatgacc
     4561 accaacaagt gtgc
//
LOCUS       DASHRQ010000124         4433 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.133, whole genome shotgun sequence.
ACCESSION   DASHRQ010000124 DASHRQ010000000
VERSION     DASHRQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.133"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(543..2048)
                     /locus_tag="U2M88_002751"
     CDS             complement(543..2048)
                     /locus_tag="U2M88_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEM8375070.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="U2M88_002752"
     CDS             complement(2045..2731)
                     /locus_tag="U2M88_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM8375071.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="U2M88_002753"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="U2M88_002753"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEM8375072.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DASHRQ010000125         4402 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.134, whole genome shotgun sequence.
ACCESSION   DASHRQ010000125 DASHRQ010000000
VERSION     DASHRQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.134"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(99..1619)
                     /locus_tag="U2M88_002754"
     CDS             complement(99..1619)
                     /locus_tag="U2M88_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEM8375073.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(1862..2047)
                     /locus_tag="U2M88_002755"
     CDS             complement(1862..2047)
                     /locus_tag="U2M88_002755"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HEM8375074.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(2031..2213)
                     /locus_tag="U2M88_002756"
     CDS             complement(2031..2213)
                     /locus_tag="U2M88_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375075.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            2454..2672
                     /locus_tag="U2M88_002757"
                     /pseudo
     CDS             2454..2672
                     /locus_tag="U2M88_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            3167..3766
                     /locus_tag="U2M88_002758"
     CDS             3167..3766
                     /locus_tag="U2M88_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEM8375076.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            3830..>4402
                     /locus_tag="U2M88_002759"
     CDS             3830..>4402
                     /locus_tag="U2M88_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage abortive infection AbiH family
                     protein"
                     /protein_id="HEM8375077.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         1403 a          799 c          785 g         1415 t
ORIGIN      
        1 ataattcagt agttttgagc gtaagcgtgt catagaatgg tacctaaaca caaatagccc
       61 gatttgatac actcaaatca ggttatttgt ggcttatatt atttacagat ttcttgtatc
      121 tgctgattcc atggcaaata agcgcgtagc gcttcaggtg tcatcgaaga cagattggga
      181 atctctgtaa aaagatgatt taaatatttg cggatgttta acccattcgc aacagctgtc
      241 tggataatac tcaatataat tccatttgat ctagcgcctt ttaagctttt agaaaatagc
      301 cagttttttc gaccgatcac taactctttg atggcacgct ctgctcggtt atttgaaagt
      361 tccaagcgtc catcaagcaa cacaatctga agtccgtctt tatgattgac tgcgtattga
      421 accgcctttc caagctgcga ttttggcaaa taagcaaaac tctcacacca atgaaaaaat
      481 tcagtcatca caggctgcaa cgtttccttc ctcgctacaa acctttcttc tgcgctaagc
      541 ttattaaagg tccgttcgat tcgaaacatc ttgtcacaga agtctaatcc tattttggca
      601 tgactttttg gggacggttg tttacccaat gcttcaaaaa actttctgcg aacatgcgcc
      661 caacaataaa cattgtttac cggcagcttt ccataggccg cgtaaccatc agtatgcaag
      721 aaaccgctaa atgattctaa aaaacttttt ggctcatcaa cggaacgcga atttgcgtgc
      781 tcgtagagca caacttgttt ttcttcgctt ttacctgaag catataacca gtaaaaggtc
      841 ttttctttat gagtattcaa tacccgatag cttgtctcat ccgcgtgtaa gacctcctga
      901 ttgcttaatt cttgatgaag gcaattgtag attggctcta aaaccatttc acttaccttg
      961 atatgccaat tagtgatttt tttacgatca agagggaaac caagtgcatg ccattctttt
     1021 tcttgtcgat atgccggcac ctttttaaca aatttttccc aaatactctg tgtcacaatt
     1081 gaagaggaac ctagactatt attgatcaac ggtttaggaa caggcgcttt tttgattttt
     1141 gtttctccta atgcttcaca ttctctgcaa tgataggcat gttggtagat gacttgtttt
     1201 tctatatggg caggaacata caatggctct tctctaagcc ttgtcgcacc gatatcatgt
     1261 aaagtatgac cacagtcttc acacaaacaa tcggcacctt ttaattcata atgaatagga
     1321 acttcaggca gaccttctaa tgaaagtgct ttgagtccct tgcgttttct gcgtttaaaa
     1381 gaagataccg taatgggttc ctggctttct tgtccagtgt gctctggttc attaaaagtt
     1441 gagtcgtcta ataaatcgat ttgcccatct acgggtggtg tttctttttt tcgtccaaac
     1501 aagcgtcgat tcatttcagc aatttgttct ctaagaagag tattttcctg ttgctggatt
     1561 tcaagcaact gtgttaattg cttgatttgt acaagaagtt cagattccat ttttgtcatt
     1621 tttacaccac cattctagcg attttcttac atctattata ccattttatg acaacgaaaa
     1681 acagacaaaa tccttgattt aacaaggttt tcgtctaata tatcgcacct tttttcgttt
     1741 cttttagcgt acatttttct tcgattgaaa ggccagtcag taagacttct tttctggaac
     1801 gcggccattg aaggtaccca ctttcaaatc ttttgtacat taatacaaat ccattttgat
     1861 cctagtaaag agccttgaag cgatctttct tcgtaccgca aaataagaaa agcgcttgat
     1921 cgtaaacatc aaggtcatat tcttctataa cgaaagctgc tagtccatca attccttttc
     1981 gaagatctgt ttttccacaa acgataaaaa tatgtttcaa tgcagttgca tcaatgagca
     2041 ttggcattca actcttttaa caaggtatac aaaagatatt tatccattcc attataaata
     2101 aatatttcct tatcttccga tttgattctg caagctagtt ttggcttggt gtcaatttgc
     2161 gatttttgag ccgcacttga aaattgaact ggtacaatat cttttttatt cattcgtttt
     2221 tctccttcta tatttgatag tttaattata gaagaaagat aatcggcgta cgagatacta
     2281 tcctatgaca cgcttacatt taatttaata ttttgcctca aattagccac actttttata
     2341 gaactaagag gaaaaatgaa gttttcttcc tattaatata gttgtttgaa atcttaaata
     2401 tagcgtttta cgatgtaatg ggaagtatgg tgaataaatc aactatgttc gtacgaagca
     2461 tttactcaac taatttaatc gaatctttca ataaacaaat caagaaatac agccaccgca
     2521 aggaacagtt ccaaaatgaa gagtcgatgg aacgtttctt agtctcgtct tttgatactt
     2581 acaaccaaaa attcctaggt cgcagtcata aaggtttcca acaggccgaa ggcgagctcg
     2641 aacaaatgct gagtcaaccg atggagaatt agaaacaatc tacagtaggg aagaaccatt
     2701 gacacaaaat tattgacgct cccaactaaa agtgtcaaaa taatgatgaa atcatttagt
     2761 tcttataaat tagaaaagag aaaataaata ttccatatct actttattgg atccgtaaca
     2821 tgactaaatt ctgaatactt tttaaagctt atcatttggt aaaactgaat ataagttaat
     2881 tatatagact gaaaattttg aattttcaga atagaataac ttaaaagtac tgacaaaatt
     2941 ttttaaaggg gcaatattaa tgttctgaga taatcaaatc ctaccgaaaa tatcaaaaat
     3001 ccaatgtgac acaatcagat tgtgtctcat tcgagggaat cacacaatcc cacgtggctc
     3061 taggcagaat tgaatttttg cttggggctt tttccttata ttgattttga atgagactta
     3121 ttatatgtta taatgcaatt aagtatcatt ggagagggcg aagaaaatga agacggtaga
     3181 acctattcga gacaaaaaaa agatcgatgc gatgaaggca atcttggctt cgggaaaata
     3241 tggccaacga aatttggttc ttttttcgat tgggatcaac accgcctatc gcatttctga
     3301 tttgcgtcaa ttgaagctgg ccgacgtgct ggaaatttca cgcggtcgcg tggtggtcaa
     3361 agatcgatta gcgatgaagg aacaaaaaac agctaaacat aattcggtct atatttccaa
     3421 caaattgcga aaaacgattt tggagtacgt tcaacaggaa tttcctgatc aactccttca
     3481 gcaagatttt gacggttatt tgttccctag tcgaaaaggg acgaatgaac cgctcaaccg
     3541 ccaaagtctg tggcgaatca ttcatgatgc tggcacggca atcggattga aagacattgg
     3601 cccacattcc atgcgaaaaa cgttcggtta ctttctctat aaacagggga ccaaaacaga
     3661 aatcattcag tccttgttga accattcttc tcagcgcgaa acattgcggt atattggtat
     3721 tacacaagaa gacaaagata cagctgtaaa aagcttagat ttataatata aaagttaagt
     3781 ttaaacatgg atagttttaa attagtagat tgagtgttgg gaggaaagaa tgagtacatt
     3841 atatattata ggaaacggtt ttgatttaag tcatggttta aaaactagat acactgattt
     3901 ttcaaaatgg gttgaaaaaa gtcatagtga aatattcgat aaaatagaca caatgataaa
     3961 tgaaaataat gaggtgagaa gtacagctga tgataagata ggttggttta attttgaaag
     4021 tagtttgcat gagttaattc aaagtccagt ctttgaagat tatgtagaag agtctaagga
     4081 ttatctcata ggttatggac atgaagattg gtcagattct gcccatcatg attttcaaaa
     4141 tatggtttat gattatgttg tttctctaga agaattacaa caacttttca aggaatggat
     4201 acgcaaagaa atacagccga caatagaaca taaaattcca aaataccaat tttcgtccga
     4261 tgtactattc tttacattca attatacaga tgttttggaa attctataca agcaaccgaa
     4321 agaaaacata tatcatattc atggcagtta tgacgatata ataatcggcc actccgagaa
     4381 ttttgacaac tttataggag at
//
LOCUS       DASHRQ010000126         4374 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.135, whole genome shotgun sequence.
ACCESSION   DASHRQ010000126 DASHRQ010000000
VERSION     DASHRQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.135"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(83..1051)
                     /locus_tag="U2M88_002760"
     CDS             complement(83..1051)
                     /locus_tag="U2M88_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEM8375078.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1498..2904)
                     /locus_tag="U2M88_002761"
     CDS             complement(1498..2904)
                     /locus_tag="U2M88_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEM8375079.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(3126..3488)
                     /locus_tag="U2M88_002762"
     CDS             complement(3126..3488)
                     /locus_tag="U2M88_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375080.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3492..3830)
                     /locus_tag="U2M88_002763"
     CDS             complement(3492..3830)
                     /locus_tag="U2M88_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375081.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERMMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3809..4228)
                     /locus_tag="U2M88_002764"
     CDS             complement(3809..4228)
                     /locus_tag="U2M88_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375082.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4221..4361)
                     /locus_tag="U2M88_002765"
     CDS             complement(4221..4361)
                     /locus_tag="U2M88_002765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375083.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1234 a          994 c          619 g         1527 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aaattttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactatttt tttactcttt
     1261 tttcagttca tttgtttgct aactcaataa tagcacttct ctttagctat gcaaatatcc
     1321 aaaaaataaa accgcaccct ttgaaaaggg cgcccccaaa cttcgctaac gctaggttgg
     1381 ccaaaaaatg gactggccat tttttagcaa atgtcagctt aataggctag tggtttagcc
     1441 gtcaactcct tatcctcggc acaaaccgcc tccggtaccg ttgactgcac atcgtaacta
     1501 ccactcgatc tcatcttcag aacgctctaa tttacgctgt gagacgtttt gacgataaaa
     1561 taaggttttc tcctgctcta acacttctaa ttcctcagaa agccccatat ccttttctgc
     1621 aagaccaaaa cggatagaca aacgagtgat cgatttttga aatgcgtttt ttaatcgctc
     1681 taaacgctgg cgtaaagagc gattttcttc tttcagcttt ttattttcca cttgtaaatc
     1741 tgctaattgt tttctaccag acaaatgacg ctcttcttcc aatgtagcta catactcatt
     1801 taattctgta ttttgctcaa gcatctgtaa atattgctca ctcatgttat cagcatttct
     1861 cttggcattt gttgctctcg tagctcgttg atacaaatca acaaaatcat tcccagataa
     1921 atgatacgaa ccatcaggaa gcctcttgaa actttctaaa ggaaccatat ctttttggaa
     1981 aatgaccgcc atctcttttt ttacattcag catatttttc ttctcttctt caatcttcgc
     2041 tctctcagaa gccatcagag ccttatcacg ctcgttttct tctctttgcc tagaaatata
     2101 cctcaactca tcagaaacgc tctcacgctc ttttacagcc gtttggcgca attcagaaat
     2161 ttcactcatg atttctttgt attcgtgaat atctttaatt ttgttggtac cgacaatatc
     2221 ccttttccaa ccgtaatcat tcattaatcg ttctatagag tcaacttctt gatttctaaa
     2281 attattgaaa agcactcgac cattatcttt atcagcgtgt atcccttgtt gaaccagtgc
     2341 tttattaaag ctcggctgct tttgaacacc ccgtttataa ccttcagcaa ccggcacaat
     2401 attcaagtgc aggtgcggac ttatttcgtc attatgaata acagcattat aaacagccaa
     2461 attagggttt ctctcttgaa actccttgta atactgaacc aaaattttat tggcaatcac
     2521 tcgattttgt tcagcttgtt gtttcgatat gccagtagaa gaaccatctg ctcgatttgt
     2581 ctggaaatca tctaaaatgc ctatttgcac aataaattct cgttgaggtt ccaacttttt
     2641 atccttgcgc actttcgcta aatagtcctc gatttttcta tcggctcttt tttgtttcgc
     2701 attatatttt tctaccactt cgccaaacaa ttcctcatat ttatctgcta gattcgtctg
     2761 ctctaaataa atattttcat ccgatttcgt gaagtcaata tgcttatgcc aatcattggt
     2821 tttctggcga tcagttaact cacgattatt atgagaaata cttgtattct tcgttccttt
     2881 tttaaatgaa atactaaacc ccaagttaaa accctccttc gcgctgtcgc taataaaaag
     2941 attgtaaacc tttctgttac tctttgtcaa agagtaacat tttcctaatt gtgacatggc
     3001 tgtcacaatt ccaaatgctc tccgagggta ttcgctgtca caagtgacaa cgaaaaaagg
     3061 agccgaaagc gtgtcacgct ctcaactcct tttacgcttt attttgcttc acgcaaaata
     3121 atcgcttatc gtaaattcac gtcattaaat attgacgtaa atttactctc actcatctga
     3181 cttttcttat tcggtatcac tccgaataag ccgtcagatt catcgggata atctcgttcg
     3241 cttggcgctg atttttcatc agcaagccac tcattatcca ctgatttttc gtgttgctga
     3301 tcgtcagaaa tcgacgatag ccaccgctca aaatcagcaa aagtttcaaa cccaaaacgc
     3361 ttgcgaagag ccttgcccat tgcagcattt tctttctcaa tcaagcgttt tttctgttgg
     3421 cgaagtttcg cttgttcttt ttttaatttt tcttcttgcg ctttaatatc tgctaaagtc
     3481 attcccataa actacatctc cttttctttg gaattaataa cggaaatttg gtttaactgc
     3541 ataacatgac tcattaattt caaccaaaat tctttttctg attcatcagt tgtttgcgct
     3601 aaatgcttat gaacaaattc attagtcaaa actgaagatt ttgctagatc agcattgata
     3661 accgagttga gaacatccat cattcgttca agcatttcac tataattatc ttcatccggc
     3721 aaattcaaat aatcagctaa ccgttcatac atcaattcaa ttctatcgac tgtttcttct
     3781 ctcaaatcaa ttttatccat gtcacacctc acttccagaa ttgccaccat tttcgatttt
     3841 ttaattcttg attttctagt aacaactgat cgtttttttg ttgctgaagc aaagtcaatt
     3901 tttgttggtc acgaatcatt tccgttaatt cagaaatatg cctatctttt tccgctaatt
     3961 gttcacgcaa aaattcattt tctttttcta acgggaaatc attgcctgca tcattgaccg
     4021 gtaaatcacc agtcaattga ccaccttttg accgataaat tgaccgcaca acaccaatca
     4081 attccttgtc taacatgata accccattat ctttataccg agcaaggtca taacctgcat
     4141 tgaccagttg attgcgcact gtatttttag atatacccaa ctcatcagca aggtttttta
     4201 tggtcatttt ttctggcaaa tcagtcatta tcttctccgt tactttcagc taccccataa
     4261 acatatttaa cagcatgctt agggataatg attggggaat caattttatt atttttcaaa
     4321 atcaactcat actgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DASHRQ010000127         4326 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.136, whole genome shotgun sequence.
ACCESSION   DASHRQ010000127 DASHRQ010000000
VERSION     DASHRQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.136"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..448
                     /locus_tag="U2M88_002766"
     CDS             <1..448
                     /locus_tag="U2M88_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM8375084.1"
                     /translation="TVTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDL
                     MDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAA
                     MNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQARVTSLEQLLSTV"
     gene            complement(1166..4315)
                     /locus_tag="U2M88_002767"
     CDS             complement(1166..4315)
                     /locus_tag="U2M88_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEM8375085.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1236 a          881 c          738 g         1471 t
ORIGIN      
        1 aacggttact cgagaagggt accgagaata caaatccaat ccaaaagaat gtgtgaactg
       61 tcctctgcta aaccaatgta ccataagtaa aaatcatcaa cgagtcatca cgagacatgt
      121 ttggggagat ctgatggatg aagtggaaca tcttcgccta acagatctga ataaatcaat
      181 ttacaaaaaa cggaaacaaa cgattgaacg aatatttgcg gatgcaaaag aaaagcatgg
      241 gatgcgttgg accaagtatc gaggcttgga aaaagtcgcg acgcatacga tgctagtgtt
      301 tgctgcaatg aatttaaaga aattagccac ctggctatgg aaaggcaagg agcctttatt
      361 tttttgttca aaaattcgaa acgaagtaga taaaaagctg ttccaagctc gagttacgag
      421 tttggaacag cttttgtcta cagtctgagg actaatacag gtagtcctct tttttgttat
      481 tttgatattc taaatcattt tttattggat tattcccttg ttcgatatag acccaaattg
      541 atctatcaat aattatcaaa ctgagtcata tagagacaaa aatttcaaaa atgacctgtt
      601 aaatggtcat cgaatatcat ttaaaatcaa tacgacggag tcagacacgg agtcaaaaca
      661 agcccgctga gagtaaaaaa tatatacagt cccataggta aagtgccaca ggactgtata
      721 tttttttatt tgagattacc tgaaatgatt ccatttactt tttatctcat tttttcctac
      781 tagttagtat caaatactct cctattttct caataaacta ttttgggtta catggtatct
      841 gaaacagcta aaaccttgtc attacagcaa aaaatcactt tatctccgtt tcatttcaga
      901 tacatttcac ttaagcggat tccaaaaaaa caaaagaaaa aggaacccct agttagccta
      961 tgctttttat tggttcctct caaaacacgg tcatataaca acggggtggg gtaaatgaat
     1021 gaaaaaggtg gaggaaacat tattcaatag ctgtcttaaa tacttcgtta taaaatgtga
     1081 acaaataaaa gacatctatc cctattttct acacttccac tttactacca tctttgaatc
     1141 gtacaatttt cttaactctt ctcgtttacc tcaattcatt ttcaagtgga atcagctcat
     1201 catctaacat tttacgccat gctcgcttca tttcttgtgg gtatttaaat ggcttcgctt
     1261 ctggatggtt ggctttatat ccttcgtaat ccttactgtt gataattgct ttcatattag
     1321 gaattggatc ttcacctggc atatattgca gattagaagc atgtaattct ttctcagaaa
     1381 caaaccactc attggaaaag acatgaatca aatcatcact cgcttgtgta aaatagcgac
     1441 gtttaatgac aaagatatct tcattactat ctaaagtccc cacatcaaac gcatttaaaa
     1501 tttgtcggta aacttctttg actccttctg ttttattcaa tttagccaaa gctttttcaa
     1561 tttcttctcg tgcatctgaa ctattcgccg cataagttcc tagtaattga tcaatataag
     1621 tagaatcaat atcatacaac ttcgtggtat tttcactata aaattcgatc gtggaaaaat
     1681 cttctccctc tttttctggt tcttcctcta ttaatgaacc cttcacggtc tcgtacacgc
     1741 caatttgttc ttccaaaagt tgaatttgat tgttcaaggt tgaagatttc accatgtcat
     1801 cattgtaatc gtcataagtc actaacgatt cataagcacg attcaattct tggaatgctt
     1861 tgacgtactc gatacggatt tcaagtggcg tatgttcatc aatggattcc atggatgaaa
     1921 cctcttttaa cttaccatat gcttgcttga atcttttttt ggattcctga taacttggat
     1981 aaatcaaacc cgattcttct tttgcttctg aatacagttt tgtcgcatcc tcgacattct
     2041 tctccattgt ggctggcttt cggaaagtaa caattagtcc ttttgttttt tctggatacg
     2101 tacggttcgt acgtgaaaaa gcttggatca atcctgcata ctctagatta cgatccacaa
     2161 atagggtttg aatcgtaggt gcgtcaaaac ctgtcaatag acgatccact acgatcacta
     2221 aatcaatttg tttcccaaat tgtttgaatt ccgctctttt tcgtgccaaa cgattattga
     2281 tgtctccgtt ataacgctca atgtctgcta atgaccagga agtatcataa tactgattat
     2341 attcctcaat aattttttgc atttcttctt gttggacact tgaatctcgg ctgttttctt
     2401 ctaatgaata ggtaatagcg attcgtggaa aatcaggatc ctccatcgtt cttccttcac
     2461 gaatgggctg attaggaaac tcttttgtta accagtcagg ttctttcgtc atttctttga
     2521 tcgctcgata atactttttg gccatgtcaa tggaactcgt ggttaagatc gcagacttcg
     2581 ttggacggcc atttcgaaaa tcaaattttg tataggcatt gtctggacgg aacattttat
     2641 gaatgacttt ttggatatgt tcatctgctt caaaaacagt agggtccaaa taactttctt
     2701 ttttgacagg ctccatttga tcaatcatac gattgatctg ttccggtgaa tactctgcat
     2761 atgtttctac ttcttttaat ttccgataga ttttattctc taatgaagtc ggctccacgg
     2821 tgttttcatg ctctacttga aatcctaaaa ccgagccatc ttctaatgca ttcttgatgg
     2881 tataggtatg caagacctct ccatactgat catgggtcgt acgtgccaat tgccctttgg
     2941 cttgtttccg attctcaggg aaaatcggtg tacctgtaaa gccaaaccaa gtggatttcg
     3001 gaaacatttt tttgatttct tccatatttt cagcacttaa cgctcgatga cactcatcta
     3061 ctacaaacac gatatgttgc cccattaatt tttggaagcg attggttcct tttttctctt
     3121 cttgtttttt tgcatatttc aacgccgcat ctagcttttg acgggttgta ataatcacaa
     3181 catttgcatt ggcatctgcc aacagagttt cacttaactc tttggcactt cctgttccca
     3241 cgatcaagct attcgctttg gcgttcccgg aagaaatccc agtattaaat tccgaagcaa
     3301 atttcgtaaa ctctgtggtg gtttgattat ctaaatcttt gcggtccact aacatgatcg
     3361 tccgatcgat ccccgatttt cgtgctaaaa gcttagtaga tacaaaacta gtcaacgttt
     3421 ttcctgaacc agtcgcatgc cacacatatc ctgattgatg tttatttgca gcaataaaca
     3481 aggcttgaat cgcatgtact tgataaggat gtaacaccat gaggatttta ttgtcttgat
     3541 cttcactgac aatcgtataa tccgcaatca agcgatgagc atccgggata tttagcactt
     3601 gtttgcaaaa ctcatataag ttttctacct ttttattatc tttcgttcgc caactaaata
     3661 aaaacttagg atgcaaatcc tttggtaagg catttgcaaa ataccgagtg gtttgttcat
     3721 tggaaaccac aaacaactgt aaggtcgaaa agatattgtt ccgaaacata ccttcttctg
     3781 catatttttt gatttggtta aacgcctgat aaaaactatc ttttgcggtt acttgtttca
     3841 gctcgatttg gacaatcggt aatccattga tcagtaaggt gacgtcaaaa cgtcgatccc
     3901 gtgcttctat gttacttcca cgttttgcaa tttgatggac cacttcatag gtagagattc
     3961 ctccaccaat gtcttgatta gaataaagaa tgagcgagac agaacccagt tgcggatctt
     4021 cccgttcaat cgtgatccga gccattccat tttcaccttt taaccatttc gcagcatcaa
     4081 agggggtttt cgttcgtagt aacaattctg ttttgatcgt ttcaaattct ttatcggtca
     4141 aaggaaaatc attcagttct gcttgattat ttgagatgat tttttggcgt aaattgaccc
     4201 aaagatcttc ttctgatttt agatcgggac gatacgtcca ctgattatgc ccttccccca
     4261 atacacggat caactgttct tcgacttccg cttcctcacg gtgactaatt ttcatttatc
     4321 ttccct
//
LOCUS       DASHRQ010000128         4254 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.137, whole genome shotgun sequence.
ACCESSION   DASHRQ010000128 DASHRQ010000000
VERSION     DASHRQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.137"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(214..1032)
                     /locus_tag="U2M88_002768"
     CDS             complement(214..1032)
                     /locus_tag="U2M88_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEM8375086.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1193..1882
                     /locus_tag="U2M88_002769"
     CDS             1193..1882
                     /locus_tag="U2M88_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEM8375087.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            1896..3398
                     /locus_tag="U2M88_002770"
     CDS             1896..3398
                     /locus_tag="U2M88_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM8375088.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3411..3887
                     /locus_tag="U2M88_002771"
     CDS             3411..3887
                     /locus_tag="U2M88_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM8375089.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(3984..4094)
                     /locus_tag="U2M88_002772"
                     /pseudo
     CDS             complement(3984..4094)
                     /locus_tag="U2M88_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1419 a          608 c          760 g         1467 t
ORIGIN      
        1 ataatgagct acacgttttt ctttcaagtt gatcaatagc ttccacgata aaaatatctt
       61 tagataaaat caagaaccgt aggaactaca gggatatttg gtcaataaga gccaccgcta
      121 ttagtaaaaa ataagctatc aagtatgctc tcatcccaag aattgcctac ttcaatcgtt
      181 aaaaaccgtt aggtttagct aagtgggggt agttcactca atacttttat caataataga
      241 gcgcaaaact ttttctctta attcaactga atcaatatta ttgttttgtt cataaccatg
      301 aactaataga tcacaaatat aaagttgtga tatttttcca gctaaagatc caccattaag
      361 aaattcttca atggctgttt gtaaaactaa gtctgcagac tttcctatag gtgaatgaat
      421 atagttagtt atagcaataa tttttgcgcc atttttttta gctattttca atgaatcata
      481 agtatctttc gttttcccag ataaagaaaa aatgattact aaatcattaa ctgttaataa
      541 agaagcgact tgggcttgaa aatgagggtc tagtacagct tttgcttgaa ctcctacgcg
      601 aagaaacata ttttctaaat caagactagt atttccacta gatcccactc caaatatata
      661 tatgtttttt gattgagtga tcattttaat ggcttcattc aatttttctt cgtctaaaag
      721 cctaatagtg gaatagagag cttctgttaa actattagca atttcatcat agtactttac
      781 actagtcgga tgttcttttt tttgactaaa gtcttccttt gcaatttcaa tttttaaatc
      841 agaaaaacca gaaaaaccaa ttttttgaca aaagcgaata atagtagcat cgccaacttt
      901 tgtgtttttc ttaatatcac tcatcgtact ataaataata gttttcccag aatttaaaat
      961 aaaatcagcg actttttttt cagacttcgt taaaaatgga taattttctt taattaaata
     1021 gatataactc atacaaactc ctctacaatt tcaacttttc tccattatac tccaaaataa
     1081 taaaaaaaac tcctaaatac tccattttta tctttacttt tatgaaagcg gatgctatat
     1141 tgtttttgga gttgttatcc aaaaataata aaaaattgga gtgtttagga taatggataa
     1201 aaagaggttt ttagatctta tttcaaaagg tttgattgtt tcctgtcaag cattacctgg
     1261 tgaaccattt tacaaagagg aagaaagttt aatgccttat tttgctttgg ctgctcaaag
     1321 ggctggagca gttggtatac gtgcaaattc tgttcgagat attattgaaa ttagaaagtg
     1381 tgtatcatta ccaatgattg gaattattaa aagagattat ccaccagaat ctccattcat
     1441 tactgccact atgagagaag tagatgaatt agtagaaacc ggtgttgagg taatcgctct
     1501 tgattgcact cttagaaatc gacatgatgg actatctata aattctttta ttactaaaat
     1561 aaaagaaaaa tatcctcatc agttatttat ggcagatatt tctacatttg aagaaggcaa
     1621 aaatgcatat aaagcaggaa tagattttgt aggaactact ttaagtggat atactgagga
     1681 aagtaaaaaa caagatgggc cagatataga tttgattaaa aaattggtag aagaagggat
     1741 ttgtgtaatt gctgagggaa ggattcatac tccagaacaa gcaaaagaga taaaaaaaat
     1801 aggagttgca ggtattgttg tgggtggtgc tattactcga ccacaggaaa ttgcatcaag
     1861 atttgttaaa gcattaagtt agggggagaa ataaaatgtt taaaaaattt tcacaattag
     1921 gacgcgcttt catgttacca attgctatat taccagtagc cggattacta ttgggactgg
     1981 gaggagcgtt aacaaatgag agtgctataa atgcttatcc atttttagat caaccgtggt
     2041 tgcatacaat tttaagtatt atgagttatg ctggaaatgc agtttttgca aacctagcac
     2101 taatttttgc aattggtatt gctgtaggac ttgcaaatgg agataaagga acagctggtt
     2161 tagctggtgg agtagcttat ctagtatata ctgcaactat tagtgggttt cttgaacttt
     2221 tttcagcaaa agatgcaaca ctagatacag gtgtagtggg atcaattgtt gttggtggta
     2281 ctgttgcatt tttacacaat cgttatagaa aaattgaatt accacaattc ttaggttttt
     2341 ttggaggatc aagatttatt ccgattgttt ctagtttagc tgcaatcatt attggttctt
     2401 tcttttattt aatttggcca ccaattcaaa atggtttagt agttgctggt gaacaaattg
     2461 cccaaatggg tagtttagga acttttttat atggtttttt attgcgttta acaggtgctg
     2521 ttggactaca ccacaccatt taccctttgt tctggtatac atctttaggt ggtaccgaaa
     2581 ctgtagctgg atcaacaatt gctggagccc aaaatatttt ctttgcccaa ctagctgatc
     2641 caaaccacac tggtctgttt acttatggta ctagattttt tgcaggaaga tttgcaacaa
     2701 tgatgtttgg attaccagca gcatgttatg caatgtatcg tgcaattcct aagaaaaatc
     2761 gtaaaaagaa tggtgggctt tattttagcg gggcccttac atcattttta actggaatta
     2821 ctgaaccagt tgaatatatg tttttatttg ttgctccttg gctttatgtg attcatgcat
     2881 tcttagatgg attatctttt tattttgctg atatcttaaa tattcgtgta gggaattcct
     2941 tttcaggtgg tttgatcgac tttttattat tcggtgtact tcaagggaat gacaaaacta
     3001 attggttaaa attaattcct tttggtattg catgggcatt tatatatttc tttgttttta
     3061 cattctttat caaaaaattt aaggtagcta ttcctggaat ggaagaagaa tcagaagttc
     3121 attctgaaaa agtaataaac aaaaatacat cattacatga agagtctctt atcattattg
     3181 aagctcttgg tggaaaagaa aatattgagt ctgttgaagc ttgtgcaact cgtttgcgtg
     3241 ttgctgttac gaatggggag attattcaaa aacaaacaat aaaagacact ggtgcaactg
     3301 ctgtcttcga agtgaagggt ggcatccaag ctgtttttgg agggaaagct gacttattaa
     3361 gtcaagaaat caatcaaata ttaggaaaag ataattagga ggtttttaat atgtttgaaa
     3421 ttttcaaaaa gaaaaaacaa gaaacttttt atgcaccttg ttccggaaaa atcatttcca
     3481 tcacagaagt aaatgatcct gtcttttcac aaaaattact aggtgatggt tttgccgtca
     3541 tacctgatgg ttcaaaaatt tatgctccat tggctggtcg agtaacaaat atatttccta
     3601 ctaaacatgc tattaatata aaaagcaact caggcattga ttatttgatt catattggaa
     3661 ttgatacagt tgaattacaa ggagagcctt tttcgatttg tgttcaagaa aatgatgagg
     3721 ttactcctga aacctcgttg gttgatgtgg attttgacaa aataataaaa tctggtaaag
     3781 atacttctgt tatagttgtt tttccggaga aaaatcagtt caatgattta ataatagata
     3841 atggtcgaat tgctcatggg aattcatgtg gaaaaatcac tttttaaaaa agtgtatata
     3901 aaaagctata aaataagcta aataggatat ttgtcaaatg aagttggtga acagaaagaa
     3961 ctgttttctt tctgttcatt tttttatttt attagtaatg attccccatg ccaaatcatg
     4021 atccgtaatt taaagttata aaagcttcga aatccattgg catttcgttg gaataccttg
     4081 atatgattat tcaaatataa aatatagaga gaagagagag agggaagata tttataaagg
     4141 gcaaagttat gtttactcaa cttaggaaat agaaatagat caatttatat tctcttgtca
     4201 atcgaatatg aaccttagca ctatatacca aatatagtaa aatgctgaaa gatg
//
LOCUS       DASHRQ010000129         4247 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.138, whole genome shotgun sequence.
ACCESSION   DASHRQ010000129 DASHRQ010000000
VERSION     DASHRQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.138"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..156
                     /locus_tag="U2M88_002773"
     CDS             <1..156
                     /locus_tag="U2M88_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEM8375090.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="U2M88_002774"
     CDS             175..1452
                     /locus_tag="U2M88_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8375091.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="U2M88_002775"
     CDS             1462..2118
                     /locus_tag="U2M88_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8375092.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="U2M88_002776"
     CDS             2118..3494
                     /locus_tag="U2M88_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8375093.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(3780..>4247)
                     /locus_tag="U2M88_002777"
     CDS             complement(3780..>4247)
                     /locus_tag="U2M88_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM8375094.1"
                     /translation="LTDLNKSIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHT
                     MLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCDVGDI
                     SFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
BASE COUNT         1668 a          489 c          741 g         1349 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatcgcag
     3961 actgtagaca aaagctgttc caaactcgta actcgagctt ggaacagctt tttatctact
     4021 tcgtttcgaa tttttgaaca aaaaaataaa ggctccttgc ctttccatag ccaggtggct
     4081 aatttcttta aattcattgc agcaaacact agcatcgtat gcgtcgcgac tttttccaag
     4141 cctcgatact tggtccaacg catcccatgc ttttcttttg catccgcaaa tattcgttca
     4201 atcgtttgtt tccgtttttt gtaaattgat ttattcagat ctgttag
//
LOCUS       DASHRQ010000130         4225 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.139, whole genome shotgun sequence.
ACCESSION   DASHRQ010000130 DASHRQ010000000
VERSION     DASHRQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.139"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="U2M88_002778"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="U2M88_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEM8375095.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="U2M88_002779"
     CDS             complement(2064..3062)
                     /locus_tag="U2M88_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEM8375096.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="U2M88_002780"
     CDS             complement(3288..3941)
                     /locus_tag="U2M88_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEM8375097.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         1239 a          895 c          655 g         1436 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttg
//
LOCUS       DASHRQ010000131         4201 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.140, whole genome shotgun sequence.
ACCESSION   DASHRQ010000131 DASHRQ010000000
VERSION     DASHRQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.140"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(3..1223)
                     /locus_tag="U2M88_002781"
     CDS             complement(3..1223)
                     /locus_tag="U2M88_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEM8375098.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1243..1854)
                     /locus_tag="U2M88_002782"
     CDS             complement(1243..1854)
                     /locus_tag="U2M88_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEM8375099.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQTFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1897..2829)
                     /locus_tag="U2M88_002783"
     CDS             complement(1897..2829)
                     /locus_tag="U2M88_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEM8375100.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLSNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3254..4111)
                     /locus_tag="U2M88_002784"
     CDS             complement(3254..4111)
                     /locus_tag="U2M88_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8375101.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKNDTID"
BASE COUNT         1192 a          848 c          692 g         1469 t
ORIGIN      
        1 ttttatttta cagaagcact cgaaactgca tctactgcag tttcatcgtg tgtgctggcg
       61 ctggttactg catctgtttt tccaccatga acagatgcac ttgaaaccgc atcttcattt
      121 ccaacaagtt gttgacccgt ttgttgtaaa taccactcaa tgattggttt gaaatcaaga
      181 atgtattcat tgattccagc atactctcca ttttcatcat gcattgcttg gtaattatgc
      241 acaatatatt tttctggcac ttgttttggt acatgaacac gtaccgcatc tgttgctccg
      301 cttcgcagtt gttgaattac ccactctaca tttttatacg atttttctgg gtgacagttg
      361 gctaatggat tccctacctg accgggcaca cgagcagcta acatctcttt cgcttcttgg
      421 cgcaaattat aatataaaaa ttggttatta ttatctgcaa aagttaattc catcggcatc
      481 gaatctaaaa agtaattgat ttgatccact gtcaataacc cacgatttaa tttgacgtaa
      541 gtatttccag aaacggcacc gactgcttca gaagcttttt ccaaccaatg atcatctgac
      601 atatcaacat cgggtattgt cgtagcaatt gttcctgtcg catgcaaatc ttctggtgga
      661 agctttttag gccctgctaa ggtagacacg actttaatga attttacaaa acttgctaat
      721 gtactgccta aaaagtcaac agttcgtgga tcaatcaagc gatcttcttg gtcaaatgct
      781 tcttttgctt ttcccaataa aaattcattt ccgggcatca ctaatgcatt cacgcctggt
      841 gcgtccaaaa tttggcgcaa gtgtaattgc gcgcgtgaag ttccttgatc gtagtaagaa
      901 gcacccacaa ccattacagg tttattttca agtggatgga ttttaaatga tagccattca
      961 agtgcacttt tcaatcctgc tggaaccgta tgattgtgtt ctggcgtagc aataattacc
     1021 ccatcagcca ttaagatttt gcgatttagc tgttgaatag caggactatt tgtttgatca
     1081 tcatcttgat tgaacagagg taaatctttg atttcaatga tttctaaatt aaatagttca
     1141 tgatattgtt tttggataaa tgttaataat ttacggttat atgaaagttc agctgctgat
     1201 ccgacgatac ctattaattt catctttgtt tgctcccctc gattaaatcg tttcccatga
     1261 aaagctttcg gcttcacgtt tatttgcttg gtgcgcatgt tgcaattgac tagtgattgt
     1321 gacaaagagt aagaaatctt tgaatagttc acgtaatttt gcgacttgtt cttcatcaat
     1381 gagtcgtcct tgctcatcaa aggtttggag tgaatgaccc aataaaaatt ctgagcttgg
     1441 cataattcgt gcctttaact ctggtgaatc caatatttgg cgcaaatgtg cttgtgcgcg
     1501 tgaagaacct aacgtgccat aagacgctcc ggtaatcatc actggtttat ccacaaacgg
     1561 aaaaatacca tatgaaagcc aatttaatgc gttaatcaat gaagcaggaa ccgcatggtc
     1621 atactctggt gtgctgatga ttacgccatc tgcttgttca atttttccag caatttcttt
     1681 aacaacattt ggtagttctg tgttttcagg tttgttaaat agtggcagct ccttgatttc
     1741 gacaatttcg atttctgcct catttacaaa ataatttttc ataaactgta ataattcacg
     1801 gtttgttgac cgcgttgaat ttgttcctac taatccaata aatttcatct tcattctctt
     1861 tcctttctaa catcgatttc tgactgttcg cacgtactaa attttttcaa atccttttgt
     1921 atacaagaat tgattctctt ttgttacaac tgctccctcg atacccggct gttcttcaag
     1981 cgctttcaac acttctaaag aagatgctcc aaataaacgt gtagtccaga tttctccatc
     2041 tacagaagaa tcagacaaaa tcgtcaggct tgctacgtca tttttaactg gaaagccagt
     2101 tttcggatca atgatgtgat gatacactga cgaaccattt tgtagataac gttcgtagat
     2161 tccagaggta actaaagatt ggttgcttag tttaaccacc gcaagcgact cttcacgtga
     2221 acctttagga tttcgcaaac caatacgcca agcaccatcc gaacgtcttg gtgattcacc
     2281 aaaaacaacg atatttcctc ctaagttaac taaagcaaac gctgcatttc gctctcggca
     2341 atgtgtaatt aatttatctg caatgtaacc ttttgctaac gcccccaagt caatcatcat
     2401 tcctggtttg gttaaaaaaa cggttttcat tttttcatct aattgaattt ggtgtggatc
     2461 cgttagcaga agtaactcag ttatctctgc ttttgttgga accaatgcat cttcaaatcc
     2521 aatgtgccat ttttgtacaa ccggaccaat agcaatattc agataactat ttcttgcaca
     2581 actatgtgct tttcctaatt tgatgagctc aaaaagttca ggatgtacaa tcacagcttg
     2641 tcgtccagca gcttgattga tggccattaa ttcagaagtt gcatcatgtg cgctgaagcg
     2701 atgttcataa acgtgcaacc attcgcctaa ctctgctagt aatcgactag gctcttcatg
     2761 cccaacttgt aatgtaattg ttgtccccat catttgccag attttcgttt cttcgatcac
     2821 ctgattcatt ttgtaccttc tttacttgaa ctacggatta catgagtaat tagtttttat
     2881 ttttgttact gttttcacaa ccagatgata cgcctgctag atttatttgt caaccgattt
     2941 tatatatttt tttatataat aaggtaattt taatataaaa ttttatattg taaaaaaaca
     3001 aataagggaa acaaacaaca catttttgtg tgttgcttat ttccctctaa ctatcgtttt
     3061 ctatttgatg gttaacctac ttaaaataaa tactgcaacg acaaacggag atcaagcatg
     3121 ctttgtaacg tattttacga gaattacgac tttattttat tactctatct tgctgccata
     3181 cccgtctatc cctaatcatc tttggtgaat tttttcaaag ttagcataac aaaaataagt
     3241 attttttcct tcgttaatca atagtatcat tttttttcat atgatttaat tcatcaatta
     3301 attttttgtt tttattaatt ttagcaaaag ctagtgcatc ttgaatcaat tcgagcactt
     3361 ctgccttcga cttattttta gcctgtgcgt tcttcgctaa ttgggcagca gctcgtgcta
     3421 agtaataagt caaatggtta tccgaacaaa tatcgattgc gtaatttagt agtgcatcac
     3481 ttgtttccaa atcaccgtct tgcgcaaaaa aaacaccaca atgaaagacg atatttaaca
     3541 cccgccagat atcttctgtt gtacgaatcg gatacgtata aattttttca aacactcgat
     3601 tgaaataaac ttctgctttt tccaactctt tttctctagc atagaccatt ccaatacctg
     3661 tatatgccaa taaacgaaac aacgtgttgg ctgtttctgc catcaacaac tgatcaaaat
     3721 ggaacaacac atccgtaatt ggtgcattcg taaaaatcat ttcaaatcct tttagatagt
     3781 agtaacgtgc aatcagctca gactcgtcaa ttaccttggg atcaatggct gctaaaatct
     3841 gtgtcaattg ttcatattca ctcgtaatca gtgaaaactc tgctttttct agctgttcat
     3901 caagttgtgt attgatcacg ttcatcgaag aaaaaagttc aactaacgga agattgattc
     3961 gattacataa tttgattaaa atttttaacg aagggacttg accattgttc tcaaatttac
     4021 ttaatgttgc ttgtgtacaa attccttcag acacttcttt ttgagataag ttggcagcct
     4081 ttcttgccgt aataaatttt tcaatgttca ttattttctc tccaatcaga cctcacacga
     4141 aaatcatttc tgtctcgatt ttagcatgtt ttctttggga atgctttaat tggttaagat
     4201 a
//
LOCUS       DASHRQ010000132         4153 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.141, whole genome shotgun sequence.
ACCESSION   DASHRQ010000132 DASHRQ010000000
VERSION     DASHRQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.141"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..106
                     /locus_tag="U2M88_002785"
     CDS             <1..106
                     /locus_tag="U2M88_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM8375102.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(359..508)
                     /locus_tag="U2M88_002786"
     CDS             complement(359..508)
                     /locus_tag="U2M88_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375103.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            970..1182
                     /locus_tag="U2M88_002787"
     CDS             970..1182
                     /locus_tag="U2M88_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375104.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1460..3259)
                     /locus_tag="U2M88_002788"
     CDS             complement(1460..3259)
                     /locus_tag="U2M88_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEM8375105.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3383..3979)
                     /locus_tag="U2M88_002789"
     CDS             complement(3383..3979)
                     /locus_tag="U2M88_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEM8375106.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1490 a          729 c          707 g         1227 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcgtt ttttgttgta taaagacttt
     1441 tttcattaac gatttttgat tagccgactc ccaaaacaaa atttagcagt ttatctgaat
     1501 aagtgatttc ttcatctgtt tgtttcacac tttcatttag tagtcccatc tcatcggtta
     1561 tgataccatc cacaccataa aacatcatgc gactcatagc atctgaatcg ttgattgtcc
     1621 atacatatac ttcttttccg tcttggtggg cagaattaat gaagttacgg ttgatcgtac
     1681 tatattccat tgtcagaaaa tcagcaggcg tcacaggggg cccaacaata ttgaaaggta
     1741 agatgtagcc cacataaaag tttggttctg cttcttttaa atctgaaacg gtttggtacg
     1801 tcaaactttg caaaatatga tggttctcta agataacttt gcggtattta tggacaaatc
     1861 gttcgacaag gtcatccgaa tcttttttag aggtcttgat ctcaataagt aatttttggt
     1921 ttagcttatg tgctttttcc aaataagaat caaaagaaca aacaggggct tgcactccgt
     1981 tttcagtaac tgtaagattt gtcaattctt tcaatgtaag atcctgtggt gttccattta
     2041 cccctgttaa gtgacgaaga tttgcatcat gcatcactac gaaatgacca tcttttgttt
     2101 cctgaacatc catctctaca taatcaggat gatagtctct gcttgttttt tcaagtgctt
     2161 ctaaagtatt ttgcgcccca ttttgggtac tcacacctct gtgagagaca gtcactggtt
     2221 ttgtctgaac agctgaagtc agataatcca tattgtaaag acagacgcca ataccgaaaa
     2281 aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga gaaacgcaag ttgggggctt
     2341 gtgggataaa ccacttaggg atctcaggca agaagccttc gtcatccatg aaatcaacaa
     2401 tgatataaaa aatccctacg gtagacatca cgatattgaa taataaaatc ccttgtaaaa
     2461 gcgtcatagc aaacacagcg ctgatcaatg catagtctgg gaaaaattgt tcaaccatac
     2521 tttgtgccaa aataacaacg atatatccta aagaagaaag gagtaaaatc gtaccgccaa
     2581 tcacaataaa ttggcccgtg atggccaaaa gacgcgattt ggtcaatgac cagctttccc
     2641 gaatggctgc tcgaaaagga cgatcccgta gaatcatttc cggtaaagca aaaatcagtc
     2701 gaatcccaat atagccaagg aaaatataaa cgagtaaaaa ggaactgacg atgatccaac
     2761 gattagcaaa aataaaatcc ataatgaaag cggggatttt tatttttgac agtagatcag
     2821 aattaaaact taatccgctg atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa
     2881 aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag
     2941 agattggttc tttcttttta ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat
     3001 aaactaacag taaaatcgtc atcaaaacta cgattaacga gagtagaacc ccaggatgtt
     3061 gagaaaaaat tgaaggaata ttgtcataag acaaatagtc aattgcccct cggtgtaaaa
     3121 taaaacgggt cgtactgctc aataagggta aacaaataaa taaaataaag ccatgcatca
     3181 gcaagacatc acggaaataa gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc
     3241 tacgtttcaa ataattcatc ctatcacctg ctcttcacat tgtagcgcaa atctaatgaa
     3301 aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag atttaacatt gtcaatcaat
     3361 gactaatttt acaaaataaa tattatccaa gaagaccact tagatattga cctccataaa
     3421 tcaaggccat actatataag aaaatcgaca gtggtttagc aatcaaaatg attagtgtat
     3481 atcgtttcac tgacatgttt gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa
     3541 ctggaagaaa aatagctaga gcaaacagct tctcaaaacg gttttgatta tccagccagc
     3601 caatatattt gttatatgtc ttatcgctaa tcatggaaag tatgaatggc tttccataac
     3661 gacgccctag cagaaaaatg ataatcgaac caatgcagat tccaatatag ttatagataa
     3721 atccagcata agggccaaat atcaggacac cagctgcagt actgatccct ccaggaatga
     3781 ttggaataac tacttgcagg atttggatga agacaaaaat aatcggtcct aaaatgatgg
     3841 aatcacctac taatcctctt aacgcattca aatctttgaa aacacctaaa ttaataaaat
     3901 aaatcgtcag cgcgattgat aggcctaatc cgataatgga tatgaaattt atcagttttc
     3961 gagaggcagc aatattcatt attcatcaac tccttagagg ttctttgtta actatacttt
     4021 actagaaaag caactaactg cgccattttt tgatatgaca gaaaacaaac aattttgtaa
     4081 gttcatcata agtataaaag attgatctca aaaaaacatc caactaaaga cttatttaat
     4141 aacttgtttt ttt
//
LOCUS       DASHRQ010000133         3810 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.142, whole genome shotgun sequence.
ACCESSION   DASHRQ010000133 DASHRQ010000000
VERSION     DASHRQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.142"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            97..2196
                     /locus_tag="U2M88_002790"
     CDS             97..2196
                     /locus_tag="U2M88_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEM8375107.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2392..3255
                     /locus_tag="U2M88_002791"
     CDS             2392..3255
                     /locus_tag="U2M88_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEM8375108.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3360..>3810
                     /locus_tag="U2M88_002792"
     CDS             3360..>3810
                     /locus_tag="U2M88_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEM8375109.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEK"
BASE COUNT         1522 a          636 c          772 g          880 t
ORIGIN      
        1 ttaagtaaat cctaaaaata agctattggc gtgacgtata gaatatgata gattaaatgg
       61 tagcgtaaac ctagtaaagg agccgatcgc taagtgatga aaaaatgtcc aaattgcgga
      121 tcgaaaaaaa tggagcagaa tactatctgt ccagactgcg gtcatcaaat aactgcagaa
      181 gatcagcaaa taaaagaatc aattgaagaa acaaatacag atgcagctat gcaggataaa
      241 tcaactgaag atgcagtctt tcctgataca gaattaaacg atccaatcga atggtcggaa
      301 ttgaaagatt tacctctaga atctgtaatg gaattattcg aaaaagcaga accagaagac
      361 gaagaaccac ctgctcacga tcaagtaact aatatagaga aaaatgaaca gaagaaagca
      421 actgttcatg agacttcgga agaatcagga aagaatcaag aagaaacgct tgaatcaaaa
      481 caaaaaagaa gagtagctga actgaaagaa atcgtcgata atgaggaaga aaattcgata
      541 ctatccgctt atatcaaagc acatcgtgaa gatacaaaag aagagcacgc gaaagagctg
      601 ctcaaaatga tcagtgaaaa aattgcacag gaaaacaaag tagagccaga aaaggaacaa
      661 gaaactgaaa aagactctga tagagaaaac gccacaggta aagaaacagt aaaaataaaa
      721 gacgtaaaaa cggatatcac agaaaaaact gaagtattta ctgaaaaaca attagaaaag
      781 gaaaaaacga ataaactgcc ggaagcagag aaaaaagaaa tactcgaaaa cacagacaaa
      841 aagtcaacag agcccgatga aataaatagt gaatccgatg ctgaaaatca atctgtaagc
      901 aaagagggta ctgatcttga agcaaaaata gaaaaagaag agaaccagcc taagctgttt
      961 gaagcagaag cgaaatcaat aacaatcgtg ccagatactg aaaaaacaca tcaagaatca
     1021 aacttagaaa aaacagatcc agataaagcg caaaagaaaa aatcaaaaaa agtaccttat
     1081 ctcgttttag cagcagtact tctactcggt ggaggaagct gggcttatta tgaccatcat
     1141 caaaaagtag aagccgaagt agcagcagaa gcaaaaagac agcaacaaat ggaagaactt
     1201 catgagaaac ttgctgcctt ttatacagat gatcaccatc aatttattcg tgcagatatg
     1261 attcatcaag atttatcaaa actaaaaaat gatttatcca gtcttaagaa tgaaaaagga
     1321 tattcagagt tagaaacaac ttaccaagat atccaatcta agatccgaga tatccaaaaa
     1381 gtgaatgagt ggttcatagc acctgccatc gtcgatgatc atctagcaga tgacatcaag
     1441 ttgaaagcag ataaagagat ccaagcaatc gaaacggata acacagattt tggaaaattg
     1501 attcaacaag cacaaacaga agctaaaaat caatatgctg aattgcaaac agctaaagaa
     1561 aaaacagcta tagtctttaa agacggaaaa gtagtggatt cagcaacacg cgaccaatac
     1621 aagacggcaa aagaagcagt agaaaaagtg aaaaatacag atctatctgc tcagttgaat
     1681 gaacaattga aacaagtaga taagatgctc actgataaag agaaaaaaga agaagaggaa
     1741 aagaaaaaag cagaagaagc gaaaaaagca gctgaagaac aagcaaaaca agccgctcaa
     1801 gcagcagctg cagctcaagc cgctcaacaa gctgcggtcg cacaagcagc acagcaagaa
     1861 gcggcatcct cgaccccttc ctcttcaaac tcagcgaatc agccaatcaa atctgccaga
     1921 gcaagtgata ttgccgatgc atctaatcca gcatggacgt gggcaccagg tgtccaaagc
     1981 aaagtgattg ctacatgtat ccaacgtgga tatatcgttg aaggaggtta cagattagaa
     2041 aaagccagaa tcgaaaatgg ggaaggctat tataatctat atgccacttc aacaaaatct
     2101 gcattgatga aaggcattgg agaaagtgct ttgccatttt acatcgtaac catcaactgt
     2161 aaaactggat ggttcggagg aaatgggagc aattaatttt aggcaaatga aagaggctgt
     2221 gagcctgccg tttaacaccg tttattcgaa aaaggggagt atgacacaac tgttgtcata
     2281 ctcctttttt cacctttcca aaccaaggct cagctattca agtagataac attatgttat
     2341 gatagcaaag aaatgtatta agaaaacctt tcactataga gggagaaaaa aatgaacgat
     2401 ttattagcaa ctatatttac aggtattatt attgatgaaa atgaaaaaaa ctactttgtc
     2461 caaaaaaacg gacaaacctt tggcttaagc aaatcagaag gtgaacacaa aatcggtgaa
     2521 gcagttgaag gattcggtta ccaaaatcaa aaacatgaaa atcggatcac tacacaaatt
     2581 cctaaaagcc gtatcggtca ttacgctttt ggaatcgtta ctgctacacg aaaagattta
     2641 ggtgcgtttg tcgatatcgg tcttccagat aaagactttg tcgtatctct tgatgaattg
     2701 ccgacaatga cagagctatg gcctaaaaaa ggggatcgct tgatgatttc ccttagagtt
     2761 gatgcaaaag accgtatttg gggcagttta gcagatgaaa aaatattcaa atctttaagc
     2821 aaatatggaa cagaagaaat gaaaaacaaa gatgtgatag gaacagcata ccgcttgaaa
     2881 ttaacaggta cgtacgtttt aacggaagat ttctatcttg ggtttatcca tccttctgaa
     2941 cgatataatg aacctcgtct aggcgagcag ataaaaggtc gagtgattgg tgtccgacca
     3001 gacggtgtac tgaatctttc attgaagcca agagcatatg aagctatatc agatgatgca
     3061 caaatgattt taaccatttt agaacgttct gcagatcagc aaatcaactt tacagataaa
     3121 tcggatccag aagaaatcat gaaagctttt ggtatcagca aaggagcatt taagcgcgca
     3181 ttaggtaatc ttttgaaaca aggattgatt acacaagaaa acggtgtgac aaaactagtc
     3241 aaaaaatcta attgagaatc ccttgcatgt ttttcttaac tagcttataa taattttaat
     3301 taacatagtg ttatatcttt ttttatttgt aattattata aattgttggg gttgagtaaa
     3361 tggtcatgaa tgctatttcg gcgatgaaaa aaacaaaaca gcagctacat gaagcggggt
     3421 ttaagctgac accgcaaaga gaagctactc tgcttgtgct tttggaaaat gagaaagagc
     3481 atttatctgc agaagaaatc ttctttttag tgaaaaaaaa gagtccggaa attggacttg
     3541 ctactgttta tcgaaccctt gaaatcttga cagaattgaa aattgtcgat aaagtaagtt
     3601 ttaatgatgg attagcccgt tatgatttga gaaaagaagg cgccaaacat ttccatcatc
     3661 atttactatg cttgaactgc ggcacgatcg aggaagtaga agaggatctt ttacttgaag
     3721 tggagcaagt agtagaagaa cgttatcatt ttttagtaaa agatcatcgg ctaacgttcc
     3781 atggaatctg tcaggaatgc tatgaaaaag
//
LOCUS       DASHRQ010000134         3800 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.143, whole genome shotgun sequence.
ACCESSION   DASHRQ010000134 DASHRQ010000000
VERSION     DASHRQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.143"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(23..637)
                     /locus_tag="U2M88_002793"
     CDS             complement(23..637)
                     /locus_tag="U2M88_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM8375110.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIITSVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            951..1373
                     /locus_tag="U2M88_002794"
     CDS             951..1373
                     /locus_tag="U2M88_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375111.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1383..1586
                     /locus_tag="U2M88_002795"
     CDS             1383..1586
                     /locus_tag="U2M88_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375112.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1797..2381
                     /locus_tag="U2M88_002796"
     CDS             1797..2381
                     /locus_tag="U2M88_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM8375113.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            2550..>3800
                     /locus_tag="U2M88_002797"
     CDS             2550..>3800
                     /locus_tag="U2M88_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEM8375114.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLE"
BASE COUNT         1283 a          602 c          716 g         1199 t
ORIGIN      
        1 ataataaaag cagcatgatc aactatttgt cattgaccat gctgtctttt tttattctat
       61 acacggtttg tctcgtaaca ttgtagtctt tagcaatctt agcaatcgca acaccatttt
      121 taagatcttc aacgatgctt ttgtaaacaa ggcgacgttg gggatctttt gcgtctgcac
      181 tgtataattt gggccgccct ttgtagacgc cgtttgcttt tgcgatctta attccttgtg
      241 cctggcgacg tttcgattca gtccgttctt gttcagcgat catggccaaa atttggatga
      301 tcagatcctt aatgaattta tccaggagag gattgccaat tgcttcagcc atgatcggta
      361 aactagtaat gatcagcttc acatttttct tcttcaagta attgactgaa gtgatgatct
      421 cgtcataatt tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga
      481 cgtaatctaa tgcttcttgg aaaacaggtc gatgagtaat cgttgcgcca gattgtttct
      541 cgatgaaaat tctctctgca ccgaattttt tcatttcttc aatctggcgt tgttcatttt
      601 gatcgatcga tgagacacgt atgtatgcaa ctctcaaaac ttttacctac tttctgcaac
      661 tatttaaaat acaccctaat catacacgag gaagccatat ttttcaatgt tataccagtg
      721 tctatttagg gtaaacctta tatagacaaa aactaatgat ggaggaaata gtatgtgtca
      781 cttttctttt taaatggcaa tattttttgt aaataaatgt caatcaagaa aatttaaacg
      841 attatgacat taaaaatata ttttatgaat tttctagtat ataacttgat ttatctgtta
      901 agatttatta agaaaacgtt ttcttatttt tgatttcgag gtgatagaca atgaaaaata
      961 agtttatttt atctttaggt attttaacta ttggaattag tgtaactgga actacaattc
     1021 ctacatttgc tgatagtaca aatgaaccaa ctactgtagt agataataac caagaattaa
     1081 ctcctttaga taggaacgga atagccattg gtaacccaac taacgactta actgttactg
     1141 aaaattattt aatagtttct ccattacttg ctcgagcagc ttctaaaacg atacgagtga
     1201 caggaacgat gatttttgat aaagttggtt ggccttctag acctcctcaa tcacggaatt
     1261 ggaaagaaaa aagaaatggt tattggtact atggaaaatt gtatttacaa agttataaat
     1321 cagctggaaa caattggatt gcgacttatt caggaacctt aactagaaag tagggatttt
     1381 ttatgccaga atttgtgaac acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa
     1441 ttaatttacc gaaagaagaa tcagctttac aagttcagcg aactttacag gagacacaaa
     1501 aaattcaaaa atcaatttct aatctagaag attctgaaga aaaaagaaaa aaaagagacc
     1561 aaaaaacata tcaaaaagaa aaataatgat tttaaaaaaa gagtgtctag tcgacattct
     1621 ttttttctaa ttgaaagtgt tcataaaatc acccatttct ggacactttc tttttttgct
     1681 ttatttttca aaaaaacaat tcatttcatg ttcataaacc cataacaata tctatgttca
     1741 gttaaactat atggataaag ttatgttaca ataaaattaa tgaaggagtg atttttttga
     1801 aaataggata cgcccgggta tctactggtt tacaaaattt ggatttacaa gaagacagcc
     1861 taaataaatt tggttgtgaa aaaattttta ctgatcatat gagtggggca aaaagtaatc
     1921 gaccaggatt agaaatggcc attgattttg ttcgctctgg cgatacactt gtcgtttggc
     1981 gcttagatcg tttaggtaga aatatggaag atttgatttc aatcgtcaat cgactgaatg
     2041 agcgtggtgt gagttttcat agtattcaag aaaatattac gatggacaaa tcaagttcta
     2101 caggacagct catgtttcat ttgtttgcgg catttgcaga atttgagcgt aatttgatcc
     2161 tggaacgatc cgcagcagga cgagcagctg ctcgtgcaag aggacggttt ggtggtcgtc
     2221 ctgaaaaatt gtctgaacaa gatttggaat tactgaagac tttagtggat agcggtacac
     2281 ctattaagac tattgcggaa cggtggaacg tctcaagaac caccatttat cgttatttgg
     2341 caaaaatgga ggagaaaaat ggaaagacaa attctcatta gtttttgtca tttaatgctg
     2401 aaaatttttg ccatttatct agaaaagtga caatagtatg agaatttttt gtaagtgtct
     2461 actgataact tattctcaaa tatttagcta tttcttcttt acaacatcga acatatgttt
     2521 gtataatgtg gaagaatgga gtgttattta tgatggatta ttcaaaagag ccagtaaatg
     2581 attacttttt aatcgatatg aaaagctttt acgcaagtgt cgaatgtata gaaagaaatt
     2641 tagatccatt aacaacagaa cttgttgtta tgagtcgatc tgataatact ggttcaggat
     2701 tgatattagc ttcttctcct gaagcaaaaa agcggtatgg tattacaaat gtgagtagac
     2761 cacgtgactt accacaacca tttcctaaaa cactacacgt tgttccacca cgtatgaacc
     2821 tatatatcaa gcgaaatatg caggtaaata atattttcag aagatatgtg gctgatgaag
     2881 atctactgat ttactcgatc gatgaatcga ttttaaaagt gacaaaatca ctgaatcttt
     2941 ttacgactga agaaacacga agtcagcgaa ggaagaagct cgctcaaatg atccaagaac
     3001 gtattaaaga agagttaggt ttgattgcta cggtaggcgt cggcgataat ccccttttgg
     3061 ctaaattagc cttagacaat gaagcaaagc ataatgaagg atttattgcg gaatggacgt
     3121 acgaaaacgt tcctgagaaa gtatggaata ttcccgaaat gacggacttt tggggaattg
     3181 gttcgcgaat gaaaaaaagg ctcaaccaaa tgggaatttt gagcattaga gaccttgcta
     3241 actggaatcc ttatacgatt aagaatagat taggtgtgat tggattacag ctgtacttcc
     3301 atgctaatgg gatcgatcga acagatatag caatcccacc agaaccgaca aaggaaaaat
     3361 catatggaaa ttcacaagtg ttaccacgag attatactag aagaaatgaa atcgagctgg
     3421 tggtaaaaga aatggcggaa caagtagcga tacgcattag gcagcataac tgtaaaacag
     3481 gttgtgtcca tttacacatt ggcacgtcga tcttagaaac gagaccaggg ttttcacacc
     3541 agatgaagat ccctatcaca gataatacaa aggaattgca gaattattgt cttttcttgt
     3601 ttgataagta ttatgaagga caagaagtaa ggcatgttgg aataacctat agcaagcttg
     3661 tgtacaccga ttcgcttcag ctggatttat ttagtgatcc acaaaagcaa attgacgaag
     3721 aaaatttaga taaaattatt gacaagattc ggcaaaaata tggttttacg tcgattgtac
     3781 atgcttcttc aatgttagaa
//
LOCUS       DASHRQ010000135         3544 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.144, whole genome shotgun sequence.
ACCESSION   DASHRQ010000135 DASHRQ010000000
VERSION     DASHRQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.144"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..277
                     /locus_tag="U2M88_002798"
     CDS             <1..277
                     /locus_tag="U2M88_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEM8375115.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="U2M88_002799"
     CDS             420..641
                     /locus_tag="U2M88_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM8375116.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="U2M88_002800"
     CDS             657..1499
                     /locus_tag="U2M88_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375117.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="U2M88_002801"
     CDS             1562..3364
                     /locus_tag="U2M88_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HEM8375118.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYCWKYKD"
     gene            complement(3423..>3544)
                     /locus_tag="U2M88_002802"
     CDS             complement(3423..>3544)
                     /locus_tag="U2M88_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEM8375119.1"
                     /translation="SRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1274 a          500 c          726 g         1044 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactgctgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaatag atacccgtgg tgctagttaa
     3421 ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat
     3481 agaacactgt aagccagtaa tatgctttca aatctactct ctagcccttt aacagaacgt
     3541 gagt
//
LOCUS       DASHRQ010000136         3370 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.145, whole genome shotgun sequence.
ACCESSION   DASHRQ010000136 DASHRQ010000000
VERSION     DASHRQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.145"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="U2M88_002803"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="U2M88_002803"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEM8375120.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="U2M88_002804"
     CDS             complement(2261..2512)
                     /locus_tag="U2M88_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEM8375121.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="U2M88_002805"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="U2M88_002805"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEM8375122.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          920 a          740 c          626 g         1084 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa
//
LOCUS       DASHRQ010000137         3186 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.146, whole genome shotgun sequence.
ACCESSION   DASHRQ010000137 DASHRQ010000000
VERSION     DASHRQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.146"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..243
                     /locus_tag="U2M88_002806"
     CDS             <1..243
                     /locus_tag="U2M88_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375123.1"
                     /translation="ESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFMDYQKLD
                     QLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            860..>3186
                     /gene="esp"
                     /locus_tag="U2M88_002807"
     CDS             860..>3186
                     /gene="esp"
                     /locus_tag="U2M88_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HEM8375124.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1167 a          470 c          613 g          936 t
ORIGIN      
        1 gaatctatca gtcatattaa tggtagcaat tttaaacaaa cattatttaa tctgattaag
       61 ctagatgcta aaatatcaag ttatttattc tttttgatac atgatgaatt tatggattat
      121 caaaaattgg atcagttaat tgaaagagaa agctgggaag attattacgt gggtctaact
      181 ttttcagaaa agttaaacaa ctacaagtta atagattata agtatttgag tgaaagcaat
      241 taattattca tgtagagtac tagacacttt aaatatggac aattcaatta taaagaaaaa
      301 gaatcaataa tttaatgagg ctgtgacaaa tgtcattttt tgtttcgcta aaaacgagtg
      361 aaaaaatgtt gataactcca atcattcgtt taagattcta ggaatcattc agaaatgctg
      421 tgaaaccgat actttataat tataaagaag gtgatttcaa attccgaaga aggaatttga
      481 agtcaccttt ttacttatgt cacagtctca ttcagtaatg cgggataagg ctccttattc
      541 cgttatttga atactctgaa gtttaatcta agaaagggta atattttgaa aacatgcttc
      601 atcgcataga aatgaactta atttcagaaa gaatacatac atatcttttt atatgttttc
      661 agctgctgaa tggttaaaaa gcacaaatat ccgaaaaaaa catgaagaaa tgtacttttt
      721 ttgttgtaat gttcatgaaa ttctctaagt aaattagtta acataaggtt agtaagattc
      781 ctattctgtg aataatacgt atcagattta tcttttattc ttactaggaa agtaattatt
      841 aagggagttg atttgaataa tggttagcaa gaataataag agagtatttc tagaaaaaac
      901 aaagaaacga gtattgaagt acagtatcaa aaaattaagt gttggggttg catctgtttt
      961 gattggtgtt ggtcttgtct tgggaacaac tgaactagtt aaagcacaag atgaaatttc
     1021 tccaagcagc ccacttgaaa cagcgataag ctccgtacaa gtaggtgaca aagtagcttc
     1081 ggggaatact tttcaggaga atcctggtta tacgaaaaat tataactttt cggatttaca
     1141 atttagtcct caagaactaa ctggagacac actaaaaggc aatacaattg gatttgaagt
     1201 ttacggaaaa cataatattg cagcatcaac taaaaactgg gaaatccgtc ttcaattaga
     1261 tgaacgatta gctaaatatg tagaaaaaat tcaggtcgat ccaaagaaag gaattggatc
     1321 ttctagacgg acctttgtaa gaattaatga ttcgctgggt agacctacaa atatttggaa
     1381 ggtcaattat attcgtgcaa gtgatggatt atttgctggg gcagaaacaa ccgatacgca
     1441 aaccgctcct aatggggtaa ttacgtttga aaagagccta gatgaaattt ttaaagagat
     1501 aggcatagac aatctcaaga ccgatcgttt aatgtatcga atctacttgg taagtcatca
     1561 agacgatgat aaaattgtac cgggtattga tagtacgggc tactttttaa cagattcaga
     1621 tgatttctat aatagcttag atgtatctga aaataatcct gaccagttta aacatggatc
     1681 agtaagtgca aaatatgaga aacctaatac tcaaacaaaa gatggttcag gatccacagg
     1741 tgctaatgga gctataatct tagatcataa gttaacgaaa aattataatt tctcatattc
     1801 atcttccgca aagggcacgc cttggtatgc taattacaaa attgatgaac gattagttcc
     1861 atatgtagcg ggtatacaga tgcacatggt tcaggctgac aaagtaacat atgatgtttc
     1921 gtttgaatcc ggaacaaaag tagctgattt agcaatcgag cgacgggaag gtcatgaaaa
     1981 ttatggcata ggttcaataa ctaataatga tttaactaaa cttatcgatt ttgctaatgc
     2041 aagtccacgt ccagtcgtta ttcgatatgt tttacaattg actaagccat tagatgaaat
     2101 cttagaagat atgaaagcaa cagcacaagt tgaagaaaat aaaccatttg gtgaagattt
     2161 catctttgat tcttggttgt cggatacgaa taaaaaatta atccagaaca cttatggaac
     2221 aggttattat tatttgcaag atattgatgg tgatggaaac cctgacgata aagaagagag
     2281 cggagacacg aatccatata tcgggaaacc tgaattagaa gaagtatatg atgttgacac
     2341 aacagttaag gggaaagtat tcatccacga gttagcggga acaggtcaca aagcccaact
     2401 tgttgataaa gaaggtactg tattagcaga aaaaactatc gctccaaatg aaaaagatgg
     2461 ggctccaatt tcagatactg tagaatttga atttacgggt gtagattcaa gtaaattaat
     2521 cgcgaaagat gaattaaaaa tccaaatcgt ttctccaggt tttgataaac cagaagaagg
     2581 ttcaaccgtt attaaggaat caccaaaagc ggttgataaa caaaccgtgg tagttggatt
     2641 taaaccagat gctaaagaat caattcggaa taataaaaac ttacctgaag atgcagagta
     2701 ttcatggaaa acagagcctg atacttctaa cgttactgat agtacgaaag gtattgtaac
     2761 tgttaagatc ggaaatcgaa ctttcgacgt ggatgtagag tttgctgtaa aagcttctca
     2821 agctatggaa aatgatgcaa catacgtacc tataacaaca actccagaaa cgacagttca
     2881 aagtggaaaa cctacatttg ataaaccaga tgttccttta gctaaagatg ccttttcaat
     2941 ttcagatgtc tataataagg acttcggtaa tgcaagtgtt gatgcaaaca ctggtattgt
     3001 tacattcact ccagctaaag gtgtaggaga atcggagccg attactggaa caattcctat
     3061 taaaattgtt taccaagatg gttctgtagg cacgaccgat ttagcagtaa ctgtaagtaa
     3121 agatatttat gaaaatccag gagaaaacat tcctgcaggc taccacaaag taaccttcac
     3181 cgcagg
//
LOCUS       DASHRQ010000138         3165 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.147, whole genome shotgun sequence.
ACCESSION   DASHRQ010000138 DASHRQ010000000
VERSION     DASHRQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.147"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            204..1172
                     /locus_tag="U2M88_002808"
     CDS             204..1172
                     /locus_tag="U2M88_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEM8375125.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1165..2196
                     /gene="vanA"
                     /locus_tag="U2M88_002809"
     CDS             1165..2196
                     /gene="vanA"
                     /locus_tag="U2M88_002809"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEM8375126.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2202..2810
                     /locus_tag="U2M88_002810"
     CDS             2202..2810
                     /locus_tag="U2M88_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HEM8375127.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(2974..>3165)
                     /locus_tag="U2M88_002811"
     CDS             complement(2974..>3165)
                     /locus_tag="U2M88_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEM8375128.1"
                     /translation="NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEG
                     TLFGFSVCTEIKVLLGIPA"
BASE COUNT          931 a          606 c          757 g          871 t
ORIGIN      
        1 aattttttag gaaaatctca aggttatctt tactttttct taggaaatta acaatttaat
       61 attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga gccgttttcg
      121 ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt tggtgccttt
      181 cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat ggatgtgagc
      241 aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg gcaacgataa
      301 ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg
      361 tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga gccggtgtga
      421 aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct gctaagagaa
      481 tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat tatactatga
      541 tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg
      601 atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt ggtgtggtgg
      661 gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga tgtaaagtgt
      721 tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat gagttgctgc
      781 aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac tatattatca
      841 gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact gggcgcggtc
      901 cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg ggcggtgccg
      961 cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc caaaaaccaa
     1021 ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc acaccgcata
     1081 cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt
     1141 tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact gtttgggggt
     1201 tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc taacattaat
     1261 aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg gaaaatgtgc
     1321 gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact ctcgccggat
     1381 aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat
     1441 gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca aggtctgttt
     1501 gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat ttgtatggac
     1561 aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc cttttgggtt
     1621 attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt tgttaagccg
     1681 gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga attggactac
     1741 gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc
     1801 tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagctgttgg cgaggtggac
     1861 caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc
     1921 tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg aggacggata
     1981 caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc ccgtgtggat
     2041 atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct gcccggtttc
     2101 acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact tcccgaactg
     2161 attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata ggatttactt
     2221 ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact tgggataatt
     2281 tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca tacgagttgg
     2341 ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga ttgcttctat
     2401 gggacggtta ccgtcctaat cgtgctgtaa actgttttat gcaatgggct gcacagccgg
     2461 aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag atgatttcaa
     2521 aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat cttacgcttt
     2581 atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt atggatgaac
     2641 gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc
     2701 gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg cactatgtat
     2761 taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa acttttaacc
     2821 gttgcacgga caaactatat aagctaactc tttcggcagg aaacccgacg tatgtaactg
     2881 gttcttaggg aatttatata tagtagatag tattgaagag gttctgttgc aaagttttaa
     2941 ataaagaata aactccctta cggtatctat gatttaagct gggattccca ataatacctt
     3001 gatttcagta cagaccgaaa acccgaagag agtgccttct tttcgggttt tcttatataa
     3061 tcctcgaatg gcttccatgc ctttaatcgt ggtagaggca gtgcgtaaac ttcgatagaa
     3121 tttattgcgt ctctttactg gacgatggtc ttgttcaatc aaatt
//
LOCUS       DASHRQ010000139         2986 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.149, whole genome shotgun sequence.
ACCESSION   DASHRQ010000139 DASHRQ010000000
VERSION     DASHRQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.149"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            380..796
                     /locus_tag="U2M88_002812"
     CDS             380..796
                     /locus_tag="U2M88_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEM8375129.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            810..1376
                     /locus_tag="U2M88_002813"
     CDS             810..1376
                     /locus_tag="U2M88_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEM8375130.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            1399..2385
                     /locus_tag="U2M88_002814"
     CDS             1399..2385
                     /locus_tag="U2M88_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEM8375131.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(2540..>2986)
                     /gene="scm"
                     /locus_tag="U2M88_002815"
     CDS             complement(2540..>2986)
                     /gene="scm"
                     /locus_tag="U2M88_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEM8375132.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSTDTSA
                     SSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTS
                     NDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT          901 a          563 c          624 g          898 t
ORIGIN      
        1 gtaaaattaa aggaataaga tgcccgagat aagcctccat gagcaagaca caggtgtcac
       61 aacctctgtt acaacctctt gttataaacg gtgttccaga agtagcttct tcgaaaaata
      121 agccgaaatt tcacaaaaac ttgaaaagta attttcgtaa attccttctt atttcttgaa
      181 gttgcccact tctgtcacaa cctcttgtta taaacggtgt tccacctgct gcttctcggt
      241 aataagccca aagaattaga aaacatggaa agctgttttc tattcttcgt tcttattact
      301 caaagcaaga cacaggtgtc acaacctctt gtttttatgc taaaattggg tgagttaatc
      361 tggaaaaagg agaacaaaaa tgagccttaa agaatttgct gaaaaaagtc tgctcaaacc
      421 ttgggatgtt tttattattt tgcttttgac tgttctttct tttttaccag tcatgatttt
      481 tggtcttcag caaaacaccg caacagcaga aaaagaagcg gtactgcgtg tggatggcac
      541 agaaatcaaa acattccctt tgatggctgg agaagagagc aacacatata cttacaaaga
      601 tgaacacgga gattataact tgatcgagat agatggcgat cgagtacgga taaaagaagc
      661 agattgcgat gaccagatct gcgtacgtcg aggctgggca acgaaaaatg gcgaaaccat
      721 cgtctgtttg ccacataaat tagtgatcga agtccgttca acagatggag gtgacgaaga
      781 cagtctgatt tattagacct tcacacacca tgagtaaatt acaaaaaatg atttatattt
      841 cgctacttgt tgcacaagga gtgatcattg gtttattgga aaatatgatc ccttatcctt
      901 tttcttttgc gcctggtgca aaactaggct tagccaatct aatcacgatc gttgccattt
      961 ttaccatgaa aaaacaagac agcttcttac tactttggct acgactattc ctgactactt
     1021 tgctaggcgg aacgatttcc acattccttt atagtatgag tggtgctttg cttagttatt
     1081 ttggaatgtt attggtcaag caattaggac caaaacgtgt cagtatcatc ggaattagtg
     1141 cggccggcgg tttcatgcat aatgtcggac agcttttgat ggcttctttc attgcacaat
     1201 catggacagt catgttgtac ttgccagtcc tttcagtttt agggatttta tcagggattg
     1261 caatcgggat cgcagcgaat tatcttttac gacatgtgca gacacttcaa cgattccaaa
     1321 aagattatga gcagggaacc aaacaaaaat ggagcccaat gtttaccaaa aaataatcgt
     1381 cacacaagga gtttttttat gaagctacat ccaatgtggg aaaactatcc tgctttaagt
     1441 aaagaattga cagaaacact aaaacggatg gaagaaactg tccgcctgaa aaacaaacct
     1501 gtcgaacaag ccatcaaaga aacgatccat gccggcggaa agctgttgcg tcctgcctat
     1561 cagctattgt tctcacaatt tggaccagag caagatcgag acaaagccat cgctttggca
     1621 gcttctatcg aaatgctcca tacggccaca ctgatccatg atgatatcgt tgatgaagcg
     1681 gatctgcgac gtggtcagcc taatatccgt agtcagtttg gtaatgtcgt ttctgtctat
     1741 gcaggagact atttatttgt ctgctgtttt aaactattgt ctgattactc cacttccttg
     1801 aaaagtctcc aattgaattc tcgcagtatg gaaaaagtat tgaatggcga attagggcag
     1861 atggatgatc gctataatta tgaactttct gtaaaagaat atttggaaaa tatttcaggt
     1921 aagacagctg aactgttcca attaagttgt tctgttggcg cttttgaaag tggaacaagc
     1981 gagcgcttcg caaaaaaagc aggagatatc ggactttcta tcgggatggc ctttcagatt
     2041 atcgatgata ttttagatta tacaaaagag agccaggaaa tcggcaaacc tgtactagaa
     2101 gacatgcgtc aaggggtcta ttctttaccg ttgatctact ctttgcaaaa aaacaaacca
     2161 aagctactac cttatttaga gaagaaagcg gcaatgacag aagaagatgt cgacgccgta
     2221 cgcaaaatcg ttgaacatac aggaggcgtg gaagaagcta ggaagttagc ggcctcttac
     2281 acagaaaagg ctttgaagga aataaagaaa ttacctgcca cctctcttcg aacaaaagag
     2341 aatctctttt cccttaccca actgattttg gatcgcaaag actgaatagg gaaaggttcg
     2401 atacgtaaat aaatttctca gaaggaaatc tacgtgtttt ttgacaaatt gaattgtagt
     2461 tcttgtattt atgataaaaa gcaaaaataa agagcaacgt ccgttagcga cgttgctctt
     2521 ttttatcttg aatatttttt taccttttcg atttcttata actcataaaa gtgattctta
     2581 aaccgattac aagtaatata acgcctgcga gtgttatcca attattcgat tgacttccag
     2641 ttttaggaag tgctcttttt gatttgtttc cattgtcatt gcttgtttta ttagttaagt
     2701 taaagttcgt cctgttatcc gaacttgttt ttgagaagga gacacttgca tgacgtgtat
     2761 cgcttgtggt agacgacgtg tctgtactat cgcttgaagt tgatgaatct gagctatcac
     2821 ttgtggtaga ggatgtatct gtactgtcac ttgaagctga cgtgtccgtg ctatcacttg
     2881 tggtagaggt agtatctgta ctgtcacttg aagctgacgt gtccgtgcta tcacttgttg
     2941 tggtagatgt atccgtactg tcgcttgaag ctgacgtgtc cgtgct
//
LOCUS       DASHRQ010000140         2959 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.150, whole genome shotgun sequence.
ACCESSION   DASHRQ010000140 DASHRQ010000000
VERSION     DASHRQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.150"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            16..177
                     /locus_tag="U2M88_002816"
     CDS             16..177
                     /locus_tag="U2M88_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375133.1"
                     /translation="MKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            174..1478
                     /locus_tag="U2M88_002817"
     CDS             174..1478
                     /locus_tag="U2M88_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEM8375134.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            1574..2443
                     /locus_tag="U2M88_002818"
     CDS             1574..2443
                     /locus_tag="U2M88_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEM8375135.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(2555..2875)
                     /locus_tag="U2M88_002819"
     CDS             complement(2555..2875)
                     /locus_tag="U2M88_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEM8375136.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
BASE COUNT          977 a          560 c          598 g          824 t
ORIGIN      
        1 tatgaagatg aaaaattgaa aaaaagacaa gaattacttg aagaagtgta tacggaacgt
       61 tttggaacaa aagaagagag agaaacggtc cgtttttatt ctgtctccga agagaaaaat
      121 ctagatacga cattcattgc agatttgtat aaagaaaatg gggtggaact gaaatgatct
      181 caatcaccca tacaggtaat ttattcttag atacttgttt gtctattatg tatttttttc
      241 tcgtttctta tccgatttta ggcggatttg tatggtttat tggtgtctgg tgttatgtgt
      301 ttttatacaa acataagcaa aaagaatggg tagatgttcc cttgagtgtc gaaccgttca
      361 tcacgatcat ggtgccagcc cataatgaag aaattgtgat cgaagataca atcgagtatt
      421 tgatgacgaa attgaattat cacaactatg aggtattagt gacagacgat ggttcaacgg
      481 accaaacacc agaaatatta gctagactga tgaaaaaata cgctaatctt cgtgtggttc
      541 ggatcgaaaa aaataaagga aaagcccacg cgtttaatat cggacttgcc tttgcaaaag
      601 ggaaattgat tttaagcaat gatgcagaca cggtaccgga gccagatgca ctgattaggt
      661 atgtgaatta tttcattcgc cctggtgcaa gacatattgc tgcggtcact gcaaatatgg
      721 atgtccaaaa tcgtaccaaa ttgattgcaa aatctcaaac ggttgaattc tcaagtatcg
      781 tgggcatcat taaacgaaca cagtcagctg ttttcggtgg tctttatgcg tacagtggag
      841 caaatacgat gtatcgaaaa gaagccttga tcgatgtggg cggattcaga caagatcgag
      901 cgacagaaga catcagtatt gcttgggatc atcagttaaa tgactggtta tctgtttttg
      961 ctcctgagat tatctttttc atggaagtac ctgtgacgtt gaagatgcta tatcgacaca
     1021 gaaaacgttg ggcaaaaggt ggaacagaag tctggctgac gaatttcaaa aaagtgatgc
     1081 tccatccttt taaacatatt ggaagaacga tcatcttcat tgatcaaaca ttgagtattg
     1141 tctggtcaat attcttttgt atttccgtag ttctttttgc tggattaatc gggcattatg
     1201 tatatcaagg aaactacgaa cagatttata ttacttttac attcagtttt gtgttcatct
     1261 gctttgaaat ggtcgcagga tttttccagt tgctagcttc gctgattgta gatgatcgac
     1321 gaagaaaatt gaaatacctt cttttcgcac cactatatat gcttttgttc tggatagtga
     1381 acgcgataac gatcgtgacg actttcattc cagcagtcaa aacgatttta ggttatggaa
     1441 gcgggacatg gaaaagcccg gaacgtacaa aaaaataaaa gatgcaccgt tttttagaca
     1501 aacagcctgt tgtgtttatg gttcttccaa acatttttgc ttataataag aaattagtta
     1561 aaaagtagta aggatgggaa aagtgatgaa aaaaagaatg aagccgttaa tctggccaat
     1621 tgctgtttcc agtattttct tattgggaca gacagctttt gcggaaactt ctgaacctgc
     1681 tcaaacagaa ccttcaacaa ccataagctc ttctggtgct gaaaacggtg cctcggaaac
     1741 agaaatgact tccaatgtac cgacagataa ttcaggagaa acgatttcat cttcagaaga
     1801 tacatcaata aatagttcta cctcttctga ccaaatcgat catttgcaag acttggaaaa
     1861 taaaagaaaa gaactcgaag aagcattaga agctgcaaaa aacaaattga aagaagacca
     1921 agcagcaatc gatcaagatt tagctgatct taaaaataaa gtgggaaatg accgtgcaca
     1981 acttgagaaa aagcttgccg aagcgaaagc aaaagcggaa gctgtccaaa cagatcttga
     2041 cgggaagatc caacaagcaa aagaattcaa tcagcaatta aatcaggttg ctcaaacagc
     2101 ttctggaaag ctaagtcaaa ctcaagcgga tatcacagcg gccagtcaag caatccacgg
     2161 aaagatcagc gaagcacaaa cagttgctaa tgattttgaa gcaaaaaaag ctgacttagc
     2221 agctagtttc caacgaataa aagatatttt gcattcggca ggagaaaaat cagtgacctc
     2281 tggaagcacg acagaaacag ttcagcaaac gacagagcca tccaaatcaa caagtcagac
     2341 tttgccaaaa acaaatgaaa aaacagcttc tgtcagttat tccttagcag gggcgggtgt
     2401 gttagtcgca gcagcttacg gaatcatccg caaaaagaaa taaaaaagag aggatgggaa
     2461 ggtagagtac ctcgaagtta gactgaaaaa tctaattctt gagtgccact acatgtccca
     2521 tctctcttta gttattaaaa cagatgtccc ctacttatct cgtggagaca gctgttccac
     2581 tacatgtgac catcagcatg ccattgtcgc tgcctaatac ttcatagtcc atttttacgc
     2641 caatgatcgc atcagctcct aatgcttttg cacgttcggc catttcggca agtgcttctt
     2701 ctcttgcacc cattagttcg ttttcatagc tttttgagcg gccgccaaag atatttcgca
     2761 accccgcctc caagtcttta aatgcatgga tcccagtaat gacctcacca aaaacgatcc
     2821 ctttataagc tactacggct tttccctcta cactgttcgt tgttgttgta atcattcgat
     2881 cactcctctc ttttaattaa agtataacaa acttcaagaa gcactacaca tattttccca
     2941 aaattagaaa tcgccgatt
//
LOCUS       DASHRQ010000141         2862 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.151, whole genome shotgun sequence.
ACCESSION   DASHRQ010000141 DASHRQ010000000
VERSION     DASHRQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.151"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            5..2377
                     /locus_tag="U2M88_002820"
     CDS             5..2377
                     /locus_tag="U2M88_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375137.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..>2862
                     /locus_tag="U2M88_002821"
     CDS             2438..>2862
                     /locus_tag="U2M88_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375138.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYA"
BASE COUNT         1144 a          455 c          621 g          642 t
ORIGIN      
        1 ggacatggca gaagaaaaag aaccactaga caatagtcgc ttgggaaaat ccaaaagaaa
       61 attagtacga ttgcaaaatg aattaaacga acaaattgag aagatgtttg aacaccagag
      121 aaagaccaac ggtcagccaa tgaacgataa gagaaatggg cattcttggt ttaggcaaca
      181 agaacgttta gaaaacaaag tgcatagtct gagagaagag atcaagcaac aagaaaaaca
      241 ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 cctggatatg acaatcgaaa atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg actttttcag ccgctacgat ccgaaaatac caacggaaat tggaaacatt
      421 agaacagctg aaagaaagat cagaaaaagg aaaagaaaat cttcttcctg aagtacaagc
      481 aattattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 agactatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctgc
      661 gccaactgat tctttgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tagaattgat
      781 cgatcaactc aaaacgttgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaagaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccca tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 aaaggaacaa aaacaaacgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaagcaaga
     1081 agtcaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtgaaa gaacttttga attttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ctgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actacacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgggg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cttcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 ggcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctgtagaaac ggcagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtga ttttaaaagg
     2221 aaaaagttat ggaaaagaga ttcaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg aaacagtgtc agaagtggcc aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg cgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg tttgaaaatg accgaaaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagact atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ctctaagcaa caatttaaag cagccacaga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtagaa aatcttcatt ttgagacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gc
//
LOCUS       DASHRQ010000142         2778 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.152, whole genome shotgun sequence.
ACCESSION   DASHRQ010000142 DASHRQ010000000
VERSION     DASHRQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.152"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..197
                     /locus_tag="U2M88_002822"
     CDS             <1..197
                     /locus_tag="U2M88_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375139.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="U2M88_002823"
     CDS             314..811
                     /locus_tag="U2M88_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEM8375140.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="U2M88_002824"
     CDS             898..1290
                     /locus_tag="U2M88_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM8375141.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..>2778
                     /locus_tag="U2M88_002825"
     CDS             1274..>2778
                     /locus_tag="U2M88_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM8375142.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          518 c          596 g          825 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagc
//
LOCUS       DASHRQ010000143         2719 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.153, whole genome shotgun sequence.
ACCESSION   DASHRQ010000143 DASHRQ010000000
VERSION     DASHRQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.153"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="U2M88_002826"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="U2M88_002826"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEM8375143.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1065 a          420 c          556 g          678 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatca
//
LOCUS       DASHRQ010000144         2645 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.154, whole genome shotgun sequence.
ACCESSION   DASHRQ010000144 DASHRQ010000000
VERSION     DASHRQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.154"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..281)
                     /locus_tag="U2M88_002827"
     CDS             complement(<1..281)
                     /locus_tag="U2M88_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEM8375144.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
     gene            743..2125
                     /locus_tag="U2M88_002828"
                     /pseudo
     CDS             743..2125
                     /locus_tag="U2M88_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2361..>2645)
                     /locus_tag="U2M88_002829"
     CDS             complement(2361..>2645)
                     /locus_tag="U2M88_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM8375145.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          937 a          473 c          453 g          782 t
ORIGIN      
        1 aaatgagtat agtatgaatt agtaatttgg ttttcatcta agtttagctt tgatttacta
       61 aaattgatat ctcgatacat gttcaataca tctaatacaa atcgagaatc ttctttcgga
      121 aatggctcag aaagatcact ccaaatttca tcatcataat gatactcgta accttctctc
      181 aatatttcga tttttctttc ataataagat ctgttttggt cctcaaattt agccaataac
      241 tcaaattgat ttactagtga caatctctga aaactgtcca ttaaatgttt cttcctatct
      301 tgttcattaa caaaaaatta taagccctta caacgaatat tatatattaa atttaattgt
      361 aggtgaacac gaattttata aatatttatg gtgttaatga caaatgatat ccgactcctc
      421 ttcatacgtt ctggactatt tgctaataga ttaaaatttc agcatctatt tctgaccata
      481 aataaatgag ctgccgaaaa gctgccaaat cagacttgaa aataaaggat caaaaacttt
      541 taagtttctg accccgtaac tcacacttca aaatcttact taaaaaggag tctgaaagaa
      601 aaaaagagta ttcaaaattt ttttctaact agctacatca catctatgca ttgacactca
      661 aagaaccaga ataagtcatc agaaagcaag ggggtgcgga cataatcctg gacgaaaaaa
      721 taaacttagg aggaatgtcc gtgtgttttc ccacaaagag cgtgtaaggg caattcagtt
      781 atttttaaaa tacgattgct cttatgctgc aaccattcga gaattagggt atccgtcgat
      841 tggtgcacta cggaaatggt ataaagagta tttggaatcc ggagagctcc atcaggagca
      901 ccgaaaaaaa tcaaagtatt ctgaagaaca aaaacgaata gcggtgaatc attattttga
      961 atatggtcaa tgttatgcac gaaccattcg catgcttggt tatcctaata gagagtcctt
     1021 gcggcagcgg tgtgaagaac ttgcaccagg agctagaaaa ctccgaaaaa gtgcggtaaa
     1081 gttaacacaa gatcaaaaag aagctgtgtt gaaaaaattc tataaaccgc aaactaatag
     1141 gaaaaattta gccgaatctg gaggaattag tagagttact ctatatcaat ggaaaaacac
     1201 ggttcttgga aaggattttc cattgtgtat gactttaaaa aatcaggaaa aacaaaaaga
     1261 gctactttta aaggaagtag aagacttaca aaaacaagta caccaattac agctcgaaaa
     1321 agcattactc gaaggagcgg ctgaactgct aaaaaaagaa aaaggcgtca atcttctttg
     1381 tctatcaaat caagaaaaaa cgatattgat tgatgcctta cgaaatcagt tcaccttgaa
     1441 agagcttctt caacagctcc agtttcctaa aagcagctat ttttatcaaa aacaagcact
     1501 tgagaaacca gataagtatt acaaagaacg tcaaattaat tatcacgatt ttcaatcata
     1561 acttctgtgc gtatggttac cgtagaattc accaagcttt aaaaaatatg ggtaaaaagc
     1621 tttctgaaaa agttgtacgt agattgatga ctgaggaaaa tctatttgtt aaattttcta
     1681 gacgaaaaaa gtattcttcg tatgctgggg aaatttctcc tgcacaccct aatttattaa
     1741 atcggaattt taaagcaaag aagccaaatg aaaaatggtt aacagatatt actgaattta
     1801 gaattctcgc agggaaaatc tacttatcac cacttgtcga ttgttacgat ggtgctattg
     1861 ttagttggac tattggaaca aagccaactt gcttcactct atgtctaaaa agggttattc
     1921 accagataat tcagcgtgcg aaggtttctt tggacgatta aaaaacgaga tgttttatca
     1981 acgtgactgg aagaatacaa caatcaatca gtttataaac aaattggatg attatattca
     2041 ttggtataat aatcaacgaa ttaagttatc tttaggaggg ctaagccctc ttcaatatcg
     2101 gaagaagcaa agatatattt gttaaaaagg tccaaaaaaa atatccgcac cccctttttt
     2161 ttgtaaaaaa ctattgcaca atacaagtag cctacggcta tcgaaatttc cataactttc
     2221 gagcaagaac ttatctcatt caaggcttga tcttccaagt aaaacaaaaa ccagttaagc
     2281 actctgccta actggttatg actaaatttt cagttgtatc tttctctacc aacactaaat
     2341 gtcgaagagc cttttttcgt ttatgaatgt aagaccgctt ctcgagccaa tgccaaacaa
     2401 ccgagcgttc cagcgttgtc ttctaatgct ggggtcacga tatatttgct taactcaggt
     2461 gtttcgacat acccattgac caattttgca aatgcgtcgt ggacgttttg cagcatatgg
     2521 cgttgtttca tcacaccacc gccaaaaatg atcacatctg gagaaaacat caaggttgta
     2581 ttgtaggcac attgagcaat ataggaggct tcgatttccc aataaggatc atcttctgca
     2641 acctc
//
LOCUS       DASHRQ010000145         2643 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.155, whole genome shotgun sequence.
ACCESSION   DASHRQ010000145 DASHRQ010000000
VERSION     DASHRQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.155"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="U2M88_002830"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="U2M88_002830"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEM8375146.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          951 a          461 c          584 g          647 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaaaacttgg agataacatc
     2641 aaa
//
LOCUS       DASHRQ010000146         2557 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.157, whole genome shotgun sequence.
ACCESSION   DASHRQ010000146 DASHRQ010000000
VERSION     DASHRQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.157"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..740
                     /gene="panB"
                     /locus_tag="U2M88_002831"
     CDS             <1..740
                     /gene="panB"
                     /locus_tag="U2M88_002831"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEM8375147.1"
                     /translation="IIDEAGINGILVGDSLGMVCLGHEDTLSVTMEDMIHHTRAVTRG
                     AKNTLVVADMPFMSYQTSVYDSVVNAGRLIKEGRAQVVKLEGGIEVCDKIEAIVKASI
                     PVMAHIGLTPQSVNAFGGFKVQGKDKEAAKELIRAAKAVEKAGAFAVVLECVPTKLAE
                     LISKEISIPTIGIGAGAGCDGQILVYQDMLGMYSDFTPKFVKKYANLSEEMNKAFTKY
                     IEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            755..1603
                     /locus_tag="U2M88_002832"
     CDS             755..1603
                     /locus_tag="U2M88_002832"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEM8375148.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            1613..2008
                     /locus_tag="U2M88_002833"
     CDS             1613..2008
                     /locus_tag="U2M88_002833"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEM8375149.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            2210..2557
                     /locus_tag="U2M88_002834"
                     /pseudo
     CDS             2210..2557
                     /locus_tag="U2M88_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1001 a          282 c          538 g          736 t
ORIGIN      
        1 aaataattga tgaagctggg ataaatggaa tattagtagg agattctctt ggtatggtat
       61 gtctaggaca tgaagataca ctttcagtaa caatggaaga catgattcat catactagag
      121 cagttacaag aggagctaag aacactttag ttgttgcaga tatgccattt atgtcttatc
      181 aaacttcagt atatgattca gtagttaatg caggaagatt aataaaagaa ggacgagctc
      241 aggttgttaa attagaagga gggatagaag tatgcgataa aatagaggct atagtaaagg
      301 cttctatacc tgttatggcg catataggtt taacacctca atctgttaat gcatttggag
      361 gatttaaggt acaaggaaag gacaaggaag ctgctaaaga attaataaga gctgctaaag
      421 ctgtagaaaa agcaggagca ttcgctgtag tgttagaatg tgttccaact aagcttgcag
      481 agttaatttc taaagagata tcaataccta caattggtat tggagcaggt gcagggtgtg
      541 atggacaaat tcttgtatat caagatatgt tggggatgta tagtgatttt acccctaaat
      601 ttgtgaagaa atatgctaat ttatcagaag agatgaataa agcatttaca aaatatatag
      661 aggaagtaaa agatggagtt tttccaggtc cagaacatgg gtttaaaata agtgatgatg
      721 ttttagaaaa attatattaa aagggtcaag aaatatggtt atatttaaaa cagtgaatga
      781 tgttagaagt caagtaaaag agtggaaaaa acaaggctta aaggtaggat tagttcctac
      841 tatgggatat ttacatgaag gtcatgaaag tttaattaga aaagcatcaa aagaaaatga
      901 taaagttgtt gtaagtatct ttgtaaatcc aactcagttt gggaaaaatg aagatttagg
      961 tagttatcca agagacttag aaagagatat agaagtatgc acaagaggta gagcaacagc
     1021 aatatttaat cctgaagttg aagagatgta ttgtgacaat gcaagtactt ttgttaatat
     1081 aactggatta acagaaggtt tatgtggagc tagtaggcca atacatttta gaggggtttg
     1141 cactgttgta agtaaattat ttaacataat accagcagat agagcttatt ttggagaaaa
     1201 ggatgcacaa cagttagctg taattaaaag aatggttagg gaccttaata ttgatataga
     1261 cgttgtaggg tgtccaatta taagggaaga ggatggacta gctaaaagtt ctagaaacac
     1321 atacttaagt ttagaggaaa gatcttcagc tactatttta aataaatctt taactttagc
     1381 taaggaagct ttaaataatg gagagagaga cagcttaaaa ataattgaga taataagtaa
     1441 aaatataaat acttataatt tggcaaagat tgattatgta gaagttgttg attctttatc
     1501 attacaaaga gtaaattata ttgaaaaatc agttttagtt gcaatagcag tatttatagg
     1561 aaaaacaaga ttaatagata attttacatt tgaattacaa tagggggcta taatgaaagt
     1621 tacaatgtta aaaggaaaaa tacatagagc tacagttgaa caagctgagt tagattatgt
     1681 aggaagtatt actgttgata tgaatttatt agaagcagca ggaatatatg aatatgaaaa
     1741 agtacaaata gttgatgtta ataatggaaa tagatttgaa acttatacaa tagctggaga
     1801 gagaggaaca gggatgattt gcttgaatgg agctgcagct agatgtgtat caactggaga
     1861 taaaataatt ttaatggcat attgtgaatt aaatgaaaat gaggttaagg atcatagacc
     1921 taaggttgtt tttgtcgatg ataataataa agttgaaaga gtaacatcat atgaaaaaca
     1981 tggaaggtta tcagatataa ttctttaggt tttgtaatgt tatttatata tagctagact
     2041 attattgaaa tatttattga ttactaattg gatagatgtc aataaagtag acacaaaaag
     2101 agaagataca gactgtagac aaagtccatt ttatgtgaat cggctacact agactagaca
     2161 gaaaaaataa ggtgtgtaga ataataaaaa acacactgga ggatttttca tgtcaagacg
     2221 tcaacgaaga acctattcaa aagaattcaa acaacaaatc gtcaatctct atctcgctgg
     2281 taagcctcgc gcagaaatta ttcgagagta tgagcttacg ccttcttctt tcgataaatg
     2341 gatgaagcaa gcacaatcaa cgggctcatt caaaaaaaga gacaacttaa caccagaaca
     2401 agcagaattg atcgcactaa gaaagaaaaa taagcaactc gaaatggaga atgatatttt
     2461 aaagcaagcg gcgctgatat tcggacgaaa agacaagtaa ttgatgccaa caagcataaa
     2521 tattccatat cagcgatgtg caaaattcta aatattt
//
LOCUS       DASHRQ010000147         2555 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.158, whole genome shotgun sequence.
ACCESSION   DASHRQ010000147 DASHRQ010000000
VERSION     DASHRQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.158"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            16..177
                     /locus_tag="U2M88_002835"
     CDS             16..177
                     /locus_tag="U2M88_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375150.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            190..693
                     /locus_tag="U2M88_002836"
     CDS             190..693
                     /locus_tag="U2M88_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEM8375151.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            753..1142
                     /locus_tag="U2M88_002837"
     CDS             753..1142
                     /locus_tag="U2M88_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM8375152.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1129..>2555
                     /locus_tag="U2M88_002838"
     CDS             1129..>2555
                     /locus_tag="U2M88_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEM8375153.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGG"
BASE COUNT          765 a          420 c          591 g          779 t
ORIGIN      
        1 caatccttag tcttactggc aattgtggtc attggtttgt ttttaggatt taagcgggag
       61 ttttccaaac tgattggctt tttggtgatt gctttgattg cggtcggatt ggtctttaat
      121 gcttctgggg tgaaggatgt cttactcagt ttgtttaacc gaattgtcgg tgcgtaaggg
      181 ggaagcacga tggaacagat gcgtgtttat attgcgaacc tcgggaaata taacgaaggt
      241 gaattggtgg gggcgtggtt taccccacct gtcgattttg atgaagtgaa agaacgaatt
      301 gggttaaatg acgaatacga ggaatatgca attcatgatt atgaacttcc ctttgagatt
      361 gatgagtata cgccaattga ggaaattaat cggttgtgtg gcttagcgga ggaacttgaa
      421 ggaacgccag ttggtgaagt tgcttctgag attcaacatg ctttcttcaa ttcttttgag
      481 gagatggtag agcatgtgga tgatattatc tattatccag attgcgatga tatgagtgat
      541 gtagcatatt tcttgattgt agaagctggt gattttggag aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtaga gacttggaga ttggaggcaa ttatttggta
      661 acgagtcgag gtgtttttga gtatccatat taacaaaatt attgaagggt agttgatttc
      721 taaactatcc ttctttttaa aggaatgatt tgatgaagaa aataaaaagc tacaccagta
      781 tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt tcaactgcct tttcctgtga
      841 cttttaacca gatggcttgg tttgttttat ccttgctgtt tgtcatcgta tttgcccatg
      901 tgcctccact atctatgata gagggggcct ttttaaaata cttaggtata ccggtggcag
      961 ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa accattggga tttttacgat
     1021 cttttattag ctatcactta cgatttaagg tcactttcgc aggaaagaaa gtcaaagaac
     1081 agaagaaacg atgggacgaa cccattactc tggtaaggag tgtgaactat gtacccgatt
     1141 aagtatattg aaaataatct tgtctttaat caagagggag aatgttttgc ctactatgag
     1201 ttaatacctt acaattactc ctttttgtca cctgaacaga aatatcaagt ccacgataac
     1261 tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc atgccttaca aattgccaca
     1321 gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg aaattaccgg gcgattaaca
     1381 gaagtagcga aacaacgaat tgatttacag acagaggcat tagtatccat gattggcgat
     1441 agtcagattg attatcgctt ttttattggc ttcaaactga ttgcgacaga tgaagaggtc
     1501 aatctaaaga atctcaagaa atctttcttc tccggctttc aagaatttgt ctatggagtg
     1561 aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg ccgatatacc
     1621 aagcttgaaa aactaatgga aagtaaattg gctcgaagat ttaaagtcag acgagtcaca
     1681 ccgagtgatc tgatgtattt gattgaacat atctatggcg aaaagggtac accatttgaa
     1741 gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg aaactttggt gaaacgttac
     1801 gacttattac gtcccagtcg ttgtttgatt gaagaaaagc cccggtactt atgcatggaa
     1861 catgagaatc atgaatcata tgtagcgtac ttaacaatta atacaattgt gggggaaatg
     1921 gagtttccct cctcagaact tttctattac cagcaacaac aatttacatt cccaattgac
     1981 actagtatga atgtggagat tgtgacgaac aaaaaggcac ttgctaccgt tcgcaataag
     2041 aaaaaggaat taaaagactt ggacaaccat gcctatcaat ctgacaatga aacaaattcc
     2101 aatgtcttag atgcattgga ttccgtagat gaattggaaa ctaccttgga tcagtccaaa
     2161 gaatccatgt acaaactgag ctatgtggtt cgagtaagtg ccgaatcagt agatgaacta
     2221 aagcgccggt gtgatgaagt gctagatttt tatgatgata ccaatgtaaa actagtccgg
     2281 ccttttggcg atatgatggg gctacatgaa gaattcttac cttctagtaa gcgatacatg
     2341 aatgattata tccagtatgt tacgtctgat tttctcgccg gacttggttt tggcgccact
     2401 cagatgttag gggagttgga aggcatttac tttggttata acgtagatac ggggcgcaat
     2461 gtttacctga aaccggcatt ggcctcacaa ggggtcaagg gttcagttac caatgcgcta
     2521 gcagcggcat tcctcggttc actcggtggc ggaaa
//
LOCUS       DASHRQ010000148         2546 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.159, whole genome shotgun sequence.
ACCESSION   DASHRQ010000148 DASHRQ010000000
VERSION     DASHRQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.159"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            386..1966
                     /locus_tag="U2M88_002839"
     CDS             386..1966
                     /locus_tag="U2M88_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEM8375154.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            2164..2388
                     /locus_tag="U2M88_002840"
     CDS             2164..2388
                     /locus_tag="U2M88_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HEM8375155.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT          904 a          414 c          534 g          694 t
ORIGIN      
        1 agatttctct ttaccaacat catttgacaa agagcccttt tcttttagtc agctgacaaa
       61 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
      121 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
      181 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
      241 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
      301 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
      361 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
      421 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
      481 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
      541 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
      601 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
      661 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
      721 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
      781 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
      841 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
      901 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
      961 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     1021 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     1081 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     1141 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     1201 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     1261 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     1321 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     1381 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     1441 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     1501 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     1561 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     1621 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     1681 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     1741 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     1801 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     1861 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     1921 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     1981 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     2041 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     2101 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     2161 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaaaga agagatgtta
     2221 cgattatgtc gattattaga tgtaaaagac tgggacaaaa acgagcaaat tgcacaaaaa
     2281 gtacgatcaa tcatccataa agaggaaaaa gttaaaaata aagacacgcg tagaactgaa
     2341 aatgaaaaga gaaaagtaca aaagaaaaga gaaaaatcaa aaagctagaa agaaaaagag
     2401 gctgagacag aagcgtttaa ctccaagaaa taagctggaa ttcacaaaaa tttgaaaagc
     2461 aattttcgtg aatttcagct tatttccgaa ggagttgctt ctgctttcgc cgtttatacg
     2521 tgtttagagc gtgagacaaa agtgtt
//
LOCUS       DASHRQ010000149         2467 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.160, whole genome shotgun sequence.
ACCESSION   DASHRQ010000149 DASHRQ010000000
VERSION     DASHRQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.160"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(492..1784)
                     /locus_tag="U2M88_002841"
     CDS             complement(492..1784)
                     /locus_tag="U2M88_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEM8375156.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(2058..2318)
                     /locus_tag="U2M88_002842"
     CDS             complement(2058..2318)
                     /locus_tag="U2M88_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375157.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          823 a          459 c          372 g          813 t
ORIGIN      
        1 tatattataa attcacatga ttatcgaatt ttattaagtt agtttttctt gtaaatctct
       61 gttcccaatg aaactcgaga cacttatttt aaagaggaaa caatgtggta tttaaatagc
      121 cctcttgtat gggctactct gtgatactta ttagccatta aactatttta cccacatagc
      181 atatattttg aaatcgttca ttccgtaaat tcaactaaat atgcctaaaa aaataaaaaa
      241 tctaagatag aataagattt attcagtctt agatggattt atagcaactt tttcgtaaca
      301 actttaaaaa atacgaaaag aaagagctgt tttactctga ctacgttgct aaattgtcgt
      361 ttaatcattc atttaaacca aatctttttt tatagaattc gttttaatcc tcctgaatcc
      421 aaaaactatg aaaaccgtaa ttaaatagtt aagagttttt aaccaccata attctctcgg
      481 ctattttgaa atcagataaa ttttgatcgg acacatagtt gaataaacct accgttgtta
      541 cggaaggtaa atcgcatact tttcattcta gaggagaagg tctcgttgat cattaactga
      601 ttaagcacca tagcccagtt ttgaattcga ccacctgacc acttcgcgtg taattctttc
      661 gtgcgtaaat aaagtagttt taggagtgca ttctcattag agaacgctcc tttttttgtg
      721 acttttctga agctggagtg gacactttca accgcattgg tagtgtacat aatttttcga
      781 atggcactac cataatcaaa tagttgttca acatgtgcaa agttccgttt ccagacatct
      841 acagcaccag aataatgaga ccaccgattt tgaaaactgt caaaagcagc atgtgcagcg
      901 tttagagaag aagcaccgta gaactttttc atatctcggc agacttcctt atagtcctta
      961 cttggaatat agcgcaatgc atttcgaaca agatgaacaa tacagcgctg aacaattacc
     1021 gatggaaaga tcgcttttgc gccttcttcg aggccagaaa caccgtccat cgaaatgaaa
     1081 aagacatctt cgacaccacg tgctttcagt tcatcaaata cttgcatcca gcgattttta
     1141 gattctgttt gatttaacca taatcctaaa atctccttat ttcctttgag atcatagcca
     1201 agaatggtgt atacagcata ttctttggct tcataatttt ctcgtaaagt aacatacata
     1261 caatcaacga atagaaaggc ataacacttt gctagggggc gggcttgcca ttcttccaat
     1321 tcaggaagga cagcgtcagt gatatctgaa atcatttcat gggaaatatc aaagccataa
     1381 atagcttcga cggttgcggc aatatctcgt tgactcattc ctcgtgcata catggaaaga
     1441 accttccctt cgatgtcgga gacatctcgt tttctcttag gaattaactc tggttcaaag
     1501 gaagcttccc ggtctctagg aacatcaata gctacttcac caaaactggt tttaagcgtt
     1561 ttagttccat agccatttcg acggttatcg tgttccttag gctctttaga atgggcatca
     1621 taacctaaat gattattcaa ttctccttga agcatttttt caaaaagggg cccaaacaca
     1681 tctttcaaag catcttgcat gtcatcgaca gattcaggtt gataggcatt cagaatggat
     1741 tcagctaact tttcggcatc aggatttctt ttctttctag ccatcgtgta atcacctctt
     1801 gatcttattg tagaaaaaga aaaaccgcga agcaaccacc tgcctaggat taatggttac
     1861 ttacacggtt tatattacac tctcatcatt aaaaaagtat ctttcgatat tactaaacaa
     1921 tgaaattatc tatttgaaat ttaaatctga taatatattg agtgttccaa tgagggcttt
     1981 gatcctatta tccatatctt tttcagtttt aatattgata cacattataa gaaaaagaaa
     2041 cttttggtta atttatttca tcccagtgca aaatataaaa aagtaaaata gaccataggc
     2101 aatagattaa caattaatat aatacaagca aatattctat tcattgtatt tctgctttca
     2161 aaaagttcta atattgaaag tataattcca caaatgccta taataagtat gggaataaga
     2221 ttagaaaata acaccccgag ccaatctgtg aaaattacta tatttactaa tatccctatt
     2281 aacataccta tcatatttat taaatcaatt tttttcatta gatatttctc ctttattaaa
     2341 tctgaagtac ttatattaaa ataattctaa actatttacc tcaaaaagtg caataaatta
     2401 tttctgaaca ataaaatgat agtcaaaaca aaaaaatatt gttaatcgtt cagagccatc
     2461 cgagagt
//
LOCUS       DASHRQ010000150         2399 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.161, whole genome shotgun sequence.
ACCESSION   DASHRQ010000150 DASHRQ010000000
VERSION     DASHRQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.161"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            206..2068
                     /locus_tag="U2M88_002843"
     CDS             206..2068
                     /locus_tag="U2M88_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEM8375158.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2399
                     /locus_tag="U2M88_002844"
     CDS             2068..>2399
                     /locus_tag="U2M88_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEM8375159.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          715 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataatta
//
LOCUS       DASHRQ010000151         2392 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.162, whole genome shotgun sequence.
ACCESSION   DASHRQ010000151 DASHRQ010000000
VERSION     DASHRQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.162"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            20..250
                     /locus_tag="U2M88_002845"
     CDS             20..250
                     /locus_tag="U2M88_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HEM8375160.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(473..571)
                     /locus_tag="U2M88_002846"
                     /pseudo
     CDS             complement(473..571)
                     /locus_tag="U2M88_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(836..1291)
                     /gene="tnpA"
                     /locus_tag="U2M88_002847"
     CDS             complement(836..1291)
                     /gene="tnpA"
                     /locus_tag="U2M88_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEM8375161.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1534..2392
                     /locus_tag="U2M88_002848"
                     /pseudo
     CDS             1534..2392
                     /locus_tag="U2M88_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          769 a          444 c          424 g          755 t
ORIGIN      
        1 tatcactagt ttatcgagta tggatgaagc aatggatcat cctctcttaa ataaatttat
       61 taaggatctg atccttcaaa ttttttccat gatcgatgag caggaaccta ctgaatctaa
      121 acgacatcgg gcacaaggaa ttattaagat tgctaagcga atgttttcta taaagggtgt
      181 tctaagctat acagtgttaa ttcaaaagat tcccaatcat cttgtttata agaacaacgt
      241 tgaagattaa aaaagtgttt caatgctaaa attgcaaaat acttcaatgt tatgagacaa
      301 actgtctata agaatcaatt tccacccatt tcatcacttg ttaaaagttt atgatccttt
      361 tgatttttat attaatttat ctgtatttaa tacagatttt atttattagt tcatctgcct
      421 attgtatttt attttgttgc cttaccatct gaagtaaata tactcttaat tgtcaaatta
      481 agttagacag atcatcaata cttacgtaac tcaaaaatac ttccaaaggt gttcgataat
      541 ccagtgattt tctaggaaga ttatttctgc gccagcattt tctaatgttt gaatttgtgt
      601 tttgtaatct tggttgcttg tgctaattct tgaatagccg taaattgcca aaatttcatc
      661 accctttctg gacataagtt tctgacacca gttaaaatca agtctatcag taacccatac
      721 acccgtgagg acgggtgtat ttttaatccc cctagaaggg gtcgacactc gctgcccctt
      781 aaaaggggat ttttaaaaga ccgccaactg ctcatcttca ctcacttact tctctttatt
      841 ttttaaatgg ttctacgtac tctcttttac ttataatata tcgaatgcga tcatcagctt
      901 cttgttcacg gatgtatttt tcatttgtct tttgattcaa tccgactgta ctcacatagt
      961 atctcttcga ccaaaaggat ttgtttccgt atttatattt gagatttgcg tgctgctcgt
     1021 ggattagtac cgcactacga cctttcagat accccataaa actggatata catatttttg
     1081 gtggtactcg aacaagcatg tgaatatgat ctagcattgc atgtgcttct atgatttcca
     1141 catctttcat ttcgcacaac ttcctaagta tttttccaag atcttttcgt aattgcccat
     1201 agattacttt tcgttgatat tttggtgtaa aaataatatg atacttacaa ttccaacgag
     1261 tatatgctaa acttttatcg tcatttgaca tgaaaaaact cctttcgatc tgatgagcgg
     1321 ttggtagcca ctcactagta tatcgaaagg agtttttatc gctaaagata cttcttccac
     1381 gcgtagaaac gcgcggtttt cttgtttcgc acgctttacg cacaaaactg actgaagtca
     1441 ttaagaaaat aaaagggtct aaaactttta agttttgtac cgacccccaa aagttagacc
     1501 aaaaaatcta acttttggag gtcggtattt tttatggcta agtatacttt tgaacttaaa
     1561 ttaaagatcg ttcatgatta cttggatggt aaaggcggaa gtgattatct ggcaaagaaa
     1621 tattccatta aggctccatc acaagtgaag cgctggatca atgcctatca agaatttggt
     1681 gaagagggtc tagtaagaaa gcgacaaaag aaaaaatatt ctgttcaatt taagctggat
     1741 gcgatagagt tatatctaac aagtgagctg tcctatcgtg aagttgccaa cacacttaac
     1801 atgaacaacc ctaatttgat cgctagttgg atgcgtcaat tccgtgaggg tgggatcgat
     1861 ggactctcaa aaacgaaagg atgtccacct atcttgtcta agaaaaatga acccaaaaat
     1921 aagaaaaaat ctacaactaa agcaacgtct aaagaacgtg aacgcatcga ggagttggag
     1981 aaacgagtcc gttcccttca aattgaaaat gcctttttaa aagaattgag gaagctgcga
     2041 aaacaggaag cccaacaacg acgaatgaag caatcgcacg aatcatcgca agcctccgag
     2101 gaccgtttaa attagttgag cttctagaaa ctttaaagtt ccctaaggca atctttatgt
     2161 actggcaaaa acgattggac cgtaaaaatc cagatcaaga gattgaagcg gaaatgctta
     2221 aagttcggga gaagcacaag gattatggct gtttacggat gaccaatgaa ctaagaaacc
     2281 gcggtttctc tatcaataaa aagaaagttc agcgcctgat caagaagcta ggaataaaag
     2341 taacgacata cacacgtaaa tcacggcgct acaattctta tcgaggacaa gt
//
LOCUS       DASHRQ010000152         2265 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.163, whole genome shotgun sequence.
ACCESSION   DASHRQ010000152 DASHRQ010000000
VERSION     DASHRQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.163"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..562
                     /locus_tag="U2M88_002849"
     CDS             <1..562
                     /locus_tag="U2M88_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEM8375162.1"
                     /translation="NVCHTTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKG
                     RWHYLYRAIDADGLTLDIWLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAF
                     RKLQSVGLYTKTEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIY
                     KKNRRNGTLFSFSVSTEIKVLMGITA"
     gene            568..984
                     /locus_tag="U2M88_002850"
     CDS             568..984
                     /locus_tag="U2M88_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375163.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            1090..1353
                     /locus_tag="U2M88_002851"
     CDS             1090..1353
                     /locus_tag="U2M88_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEM8375164.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            1353..1625
                     /locus_tag="U2M88_002852"
     CDS             1353..1625
                     /locus_tag="U2M88_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEM8375165.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            1702..2031
                     /locus_tag="U2M88_002853"
     CDS             1702..2031
                     /locus_tag="U2M88_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEM8375166.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            2021..>2265
                     /locus_tag="U2M88_002854"
     CDS             2021..>2265
                     /locus_tag="U2M88_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEM8375167.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCG"
BASE COUNT          844 a          304 c          454 g          663 t
ORIGIN      
        1 aaatgtttgt catactacga tttatcgttg ggtgcaagag tacagcaaag tcctctatta
       61 tctttggaag aagaaaaata gacaatcctt ctattcatgg aaaatggacg aaacctatat
      121 caaaattaag ggacgttggc attatcttta tcgtgcaatt gatgcggacg gcttaacctt
      181 agatatctgg ttacgaaaga aacgggatac gcaagcagcc tatgctttct taaaacgact
      241 ccataaacag tttggtgagc cgaaagcaat tgtgaccgat aaagcacctt ctcttggctc
      301 cgcctttaga aagttacaga gtgtgggttt atatactaag acagagcacc gaactgtgaa
      361 gtatcttaac aatttaatag aacaagacca tcgacctatt aaacgacgga ataaatttta
      421 tcaaagtctc cgtacagcct cttccacgat taagggcatg gagaccattc gaggaatata
      481 taaaaagaac cgaagaaatg gaacgctctt cagcttttcg gtgtctactg aaatcaaggt
      541 attaatggga ataacagcct aagatatttg gagttcagag agggcgcgtt tgattttcaa
      601 acttcgcaac agaaccgaaa atttataccg gaagattttc aaaacttaca gttagaaaac
      661 atgcgtttag aagatttatt tagattagca gatgatgata agtatagatt tgaagttttg
      721 aaaattaaaa agaatgaact attgaataag ttatatcctg agaatgcgat aaaagcacat
      781 tttggtgatg ctaatcatat gtatagttgg ttcattccta aaagatttag agggaaaatc
      841 aatgctggag atcaagtgaa agtactcaat cgaaatagta cagatgtcgt aatgataaca
      901 gatatctacg tagaagatga agttataatc aaatatatga agcctgtgat aaaacttgtt
      961 aataaaaata aaggaaacgg ataacacttt acaatgctgt tttggtactc caatttaaac
     1021 aaaacaatcg aatttacgta cttaattaag tacgttagaa tggagatata aattagaggg
     1081 ggagtagata tggcaaaaac agtcttaaat cctagctcag cacggaaaaa tttttataag
     1141 ttgttgaaag atgtaaatga gaatcatacg gaaattgaaa tcattagtga gcgcagtgaa
     1201 aataatgcgg tgcttattgg tttggatgat ttgaaagcaa tcaaagaaac gctattactt
     1261 gagcaaatag gcactttggg tgttgttcgt aagcgagaaa aagatgatag tggatttact
     1321 aatgttgatg aaattgattg ggataactta taatgaataa ctattctgtg atgattaaaa
     1381 actcggctaa agctgattta aagaaaatca aacagtctaa tttgaaacct caatttgaga
     1441 aggttattca gactttaaag gaagatccct atctccctac ccaatcattt gagaagttaa
     1501 aacctacata tgaaggtagg tattctagaa gattgaatag acaacaccgg gttgtttata
     1561 aggtagatga agatgaacac gtggttgaaa tttattcagc aaggacgcat tacgagtcac
     1621 agtaattact tgtaaataca ttatctcgtg gtataatgta tttactaagt aattacatac
     1681 tgttagaaat gagggattta aatgattaca aaaacaagaa aacaaggcaa ttcaatcatg
     1741 ttgacagtac ctaaagattt taatgttcct aatggagtcg aagttgaagc aaagttggta
     1801 gagaatggta ttctttatga atttgttgaa ccacaaaaag aattttatga ttttagcgaa
     1861 gatattttgt ctgatataat tgcagaaggc tacgataaag atgagattct tgtagaattt
     1921 aagaatagaa aaaacaaaat gcattcttcc tttagagata ttgctgaaga tacacttact
     1981 aattctaaag taatgactaa agaggagttg gcaacagaaa ttggcttatg aaatcgttcc
     2041 ttcaaaacat gtcattaagt atttaaaaaa gctaaaagag aagccattga aagaaaaatt
     2101 tttgaattta atttatgatg aaattgcgat tgatccatat aagggtgaac aaaaaactgg
     2161 ggatttatca ggtatttggt ctagtgggtt caaatatgcg ggcacaacat atagagtagc
     2221 ttacgagatt atagaaaaca aggttattcc agtattgtta tgtgg
//
LOCUS       DASHRQ010000153         2194 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.164, whole genome shotgun sequence.
ACCESSION   DASHRQ010000153 DASHRQ010000000
VERSION     DASHRQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.164"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     misc_feature    109..234
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            278..802
                     /gene="infC"
                     /locus_tag="U2M88_002855"
     CDS             278..802
                     /gene="infC"
                     /locus_tag="U2M88_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEM8375168.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            874..1074
                     /gene="rpmI"
                     /locus_tag="U2M88_002856"
     CDS             874..1074
                     /gene="rpmI"
                     /locus_tag="U2M88_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEM8375169.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            1127..1486
                     /gene="rplT"
                     /locus_tag="U2M88_002857"
     CDS             1127..1486
                     /gene="rplT"
                     /locus_tag="U2M88_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEM8375170.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            1838..>2194
                     /locus_tag="U2M88_002858"
     CDS             1838..>2194
                     /locus_tag="U2M88_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEM8375171.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLG"
BASE COUNT          735 a          384 c          479 g          596 t
ORIGIN      
        1 aatcactgct agaaagcctg ctaatctaat cattttcaga aaaggaagac aaactgtatg
       61 aaaaacattg acaaaaagat tagtttttgc tatactataa tggttgagaa ataaaagcag
      121 aagcacccgc ttctcgcctt agttgataat gtcactgggc tagatagttt tcactggttc
      181 tattaggtga agtacgcgcg ggttctgtaa gagaactcgt ttttttattg ccgtttattc
      241 ggtaagcttt tctttctctc ataatatttg gaggtgaatg accatagcaa aggatatgat
      301 ggttaacgac ggcattcgtg cacgagagtt acgattgatc ggttcagatg gtgagcaatt
      361 aggagttaag acaaaagcag aagcattgaa tattgcagaa caagcaaact tggatcttgt
      421 gttagttgcc cctggtgcga aaccaccagt tgcgcgaatc atggactacg gtaaataccg
      481 tttcgaacaa caaaagaaag accgcgaagc gcgtaaaaaa caaaaagtga tcaatgtaaa
      541 agaagttcgc ttaagtccta cgatcgactt gaatgacttc aatacaaaac ttcgtaatgc
      601 acgtaagttc ttggaaaagg gcgataaagt gaaagcttct atccgtttca aaggccgtgc
      661 cattacccat aaagaaattg gtcaaaaagt cttgaatcgc ttagctgaag aaactgcgga
      721 tattgcaaca gtggaacaaa aagcgaaaat ggacggacgc agcatgttct tgacgctggc
      781 accgaaaaac gacagtaagt aatttgaatg actaactggc tgttggacag ccgatagtcg
      841 gataacgaaa gattttgagg aggaaatata gtcatgccaa aacaaaaaac acaccgcgga
      901 tcagcaaaac gtttcaaacg tactggtaaa ggcggattga aacgcttccg cgcgtttaca
      961 agtcaccgtt tccacggcaa aacaaaaaaa caacgccgtc aattgcgtaa agcaagcatg
     1021 gtttctagtg gcgacttcaa acgtattcgc caacaactag caaaaatgaa ataagaagta
     1081 caaataacag aatctgaaga tttattttta ttaggaggaa ttatacatgg cacgtgttaa
     1141 aggtggaaca gtaactcgta aacgtcgtaa aagagtgcta aaattagcaa aaggttacta
     1201 cggttcaaaa cacactttat ttaaaacagc aaaagaacaa gtaatgaatt catacaacta
     1261 cgcatatcgc gatcgtcgtc aaaagaaacg cgacttccgt aaattgtgga ttgctcgtat
     1321 caacgcagca gctcgtatga atggcttgag ctactcaaaa ttaatgcacg gtttgaaagt
     1381 tgctgaaatc gacatcaacc gtaaaatgtt agctgacttg gctgttaacg atgcagcagc
     1441 atttactgca ttggctgacc aagcaaaaga agctttggct aaataagcta aacaattata
     1501 aagaggaaag aagataacga aaagttatgt tctttcctct ttttttattt acaatcctcc
     1561 atcaaatgaa acaggacaat aacgtataag tctaaaataa cataattaag gttttttaat
     1621 tgaaaactga acatttatgg tcatccttta atgaaagcgc taactacata agttagaatg
     1681 agtttgactg atatattagt ttgcttatca gaaaaacaaa atggacggag gaataacatg
     1741 agtgatatta ttattggtat tttgttagtt atctcattct ttttcatggt ctggtactgt
     1801 gtaaaaggct acaacttgat ggtcggcttt tgtcatcatg gctactgtat ggatgggatt
     1861 agcgctagtc ggaaacacgt tttcaccaaa ctcggcgatg gaagggcaag gagtaattga
     1921 tgtgttgacg catatctata cgacggggcc agctgagtac gccaaatcga tcttggtaaa
     1981 tgtcttcttt ggtgcctttt tcgggcgtgt tctagtagat agcggaatcg cagctaccct
     2041 gattcgaaag gtagttgaac ttggagggga caagccaaga atcacgatga gcttactatg
     2101 tattgtaacc gctgttatat tcatgtcgat gacaggaatc ggaccagtga tttctattgc
     2161 agttattgtg ttgccgatct tgatgtcatt aggt
//
LOCUS       DASHRQ010000154         2190 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.165, whole genome shotgun sequence.
ACCESSION   DASHRQ010000154 DASHRQ010000000
VERSION     DASHRQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.165"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            29..208
                     /locus_tag="U2M88_002859"
     CDS             29..208
                     /locus_tag="U2M88_002859"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375172.1"
                     /translation="MEKDIYKQLTQQIQKEKEKGLLDHLDELELKADEINKNRMSAKE
                     KGIKLKDSSLQSDKS"
     gene            694..834
                     /locus_tag="U2M88_002860"
     CDS             694..834
                     /locus_tag="U2M88_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016373449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEM8375173.1"
                     /translation="MSKKKRLTITLSEQVLEYLDEVAKKKGLSKSALITIALEEYKKG
                     QK"
     gene            900..1640
                     /locus_tag="U2M88_002861"
     CDS             900..1640
                     /locus_tag="U2M88_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEM8375174.1"
                     /translation="MTVRITKARHFSFLLYPDSIPNDWKEKLESLGISMAVSPLHDMD
                     EKKDKEKWTIDDVVRNGKHYKKPHYHVIYIARNPVTIESIRKKIKRKLGNNSVAHVEI
                     IDSIKGAYEYLTHESKDAKAKNKHIYDKKDILYINDFDIDRYITLDESQKRELRKTLT
                     NIIDMYGVVNAKELRAFLNWRGTEFGVTNLGDVEDVLSANGSLFRFYFDGNYQNGFRP
                     QYAKRINSETGEIISGVDELISKTEEGE"
     gene            1643..1861
                     /locus_tag="U2M88_002862"
     CDS             1643..1861
                     /locus_tag="U2M88_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002410865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375175.1"
                     /translation="MTKNVNDLFEEYYDNHNLDESRYSHFSKKQLVIEAEYMHDALQN
                     ILKYLDDGGTDLNAIRGEVMDGIYESRI"
     gene            complement(1917..>2190)
                     /locus_tag="U2M88_002863"
     CDS             complement(1917..>2190)
                     /locus_tag="U2M88_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375176.1"
                     /translation="NTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNS
                     KVYQEVKEKIEAKLNPSNRNRPKNHQKGTNKTVTSSNFDPTKFLKR"
BASE COUNT          783 a          278 c          468 g          661 t
ORIGIN      
        1 aataatagct tttaaggagg tgatggggat ggaaaaagat atttataaac aattgaccca
       61 acaaattcaa aaggaaaaag aaaaaggctt gctagatcat ttagatgaat tagaactaaa
      121 agctgatgaa atcaataaaa acagaatgtc agcaaaagaa aaaggcataa aacttaaaga
      181 ttcgtcgttg caaagcgata aaagctagat aacgtgcggt caacggtaaa tcgaattaga
      241 gacgcttaga gaggctaata agccctctta gcgtctcttt gaggctttaa ggagttgacg
      301 gctataaaca aaacccaaaa gctggaaaca caagaaggtg accataggga acgttcttga
      361 cgccttaacg gcgagtggaa agtttttggg gtgccttttt tcaaaaaggt acggtcttga
      421 tttgccgagc ggtagcgctg gaaaattttt ggaaaaaatt tggaatttgg aaaatctggg
      481 gggctactac gaccccccat agtgtgccga gtgccaattt ttgtgccaaa aagtgcttta
      541 tccttactgt accaatggat agagcctttt ttttcgaccg ccatttgacc gccgtttcga
      601 ccgccgtttt taaaaaaata cgcttttagt tgcatttaat gcgtaatatg tggtattctt
      661 tttaagaaga taaataatga aaaggagaat gacatgagta agaagaaacg tttgacaata
      721 acgttgtcag aacaagtttt agaatatcta gacgaagtag ctaaaaaaaa gggtttatcc
      781 aaatctgcat taataacaat agcgttagaa gaatacaaga aagggcaaaa atgagcataa
      841 aaaaagagcg ctcccgacta aaagaccgct cactcattaa ttgataaggt gattatatca
      901 tgactgttag aattactaaa gctagacatt ttagtttttt gttatatcct gattcgattc
      961 caaatgattg gaaggaaaaa ttagagagtt taggtatttc aatggctgtt agtcctttac
     1021 acgatatgga tgaaaaaaag gataaagaaa agtggacaat tgatgatgtt gttagaaatg
     1081 gtaagcacta taaaaaaccg cattatcatg ttatatatat tgctagaaat ccagtaacga
     1141 tagaaagtat tcgaaaaaaa atcaagagaa aattgggaaa taattcggtt gctcatgttg
     1201 aaataattga ttccattaag ggtgcttatg agtatttgac tcatgagtca aaagacgcga
     1261 aagcgaagaa caaacatatt tacgataaaa aagatatttt gtatattaat gattttgata
     1321 ttgatcgtta tatcaccctt gatgaaagcc aaaaacgaga gttaagaaaa actttgacaa
     1381 atattattga tatgtacggt gtggttaatg cgaaagaact tagagcattt cttaattgga
     1441 gaggtacaga atttggtgta accaacttag gagatgtaga agatgttttg agtgcaaatg
     1501 gtagtctgtt tagattttat tttgatggga actatcaaaa tgggtttaga ccacaatatg
     1561 caaaaagaat aaattctgaa actggggaaa ttataagtgg agttgacgaa ttaataagta
     1621 aaactgaaga aggagaataa gaatgacaaa aaacgtaaac gatttgtttg aggaatatta
     1681 tgataatcat aatttagatg aaagtcggta tagtcatttt agtaaaaagc aattggtgat
     1741 tgaagctgag tatatgcatg atgctttgca aaatattttg aaatatctcg acgacggtgg
     1801 aacggatttg aatgcgattc gtggagaagt catggacggc atatatgaaa gtaggatatg
     1861 aaaaaataga atattgggaa ataaaaaaca gataggaata actcctatct gtttttttat
     1921 cgttttagaa atttagttgg gtcgaaattg ctagaagtga ctgttttgtt tgttcctttt
     1981 tgatgattct ttggtcgatt tcgatttgat ggatttagtt tagcttcgat tttttctttc
     2041 acttcttgat atactttaga atttgtgcca ataagaacgc ctgtatcttt tagatataaa
     2101 tcagcgaatt ctgcgatgat ttcagtcatg atggaaattt cttttcccat agcatttagt
     2161 tttgcctgat tgctggtagt ggtcgtgttg
//
LOCUS       DASHRQ010000155         2190 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.166, whole genome shotgun sequence.
ACCESSION   DASHRQ010000155 DASHRQ010000000
VERSION     DASHRQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.166"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            353..1081
                     /locus_tag="U2M88_002864"
     CDS             353..1081
                     /locus_tag="U2M88_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEM8375177.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNRSIILNDP
                     NWCKQANLREISMPSAWEETKGSKDVKVAVIDSGIDYQHEDLSGNVDESLGYDFFDDD
                     TDPMDTMGHGHILPGLLVLKQTTN"
     gene            1111..>2190
                     /locus_tag="U2M88_002865"
     CDS             1111..>2190
                     /locus_tag="U2M88_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEM8375178.1"
                     /translation="MVPIRVFGSSGGTSLAILTEAINYASQKGFPIINFSIGSVVYSS
                     TEKAIKNYPGLFVAAAGNNGIDMTDKPFYPASLDIPNIITVGNSSGYERSSDSNYRKT
                     GVDLFARGFRVFSTFLGSKYTYMSGTSMATPHVTGSAALALSLDPDLSTDELKKIILN
                     SVDSFYELSDLCLTGGRLNTNNVVRQVTGKLTASNITLRLNSEWSDEIAKEMCHVHWI
                     DQSGNDLTDQVVVLKNEVDTSNFGDYEVVFASPDLTDQISVTVTVPRLRKLTLGKETA
                     TIDIGSAWDSVIAIDTFQVKAIDDFGNDVTNQVQVTINADIDTINSIQAVIKFEVPEL
                     GLAQIGRLNITAKNSTINGLNEVSIE"
BASE COUNT          769 a          289 c          470 g          662 t
ORIGIN      
        1 cgtggagaaa taataagaac taccagtcag ataaaacaag ataccgaaga ctcactgcgc
       61 aaagggtggg gaatgaataa taaagttaga taataatcaa agaagaacta agtaactacc
      121 tttaaacggt agttacttag ttcttctttg atttgcgtga aatagagggg atttattttt
      181 ctggttacgt gattaaaaaa tccaaaaaat gagcatagga acggttatga ttttagggtg
      241 tgttttaatg agatattgtg atttcattaa caagtgtatg ataatctgtt gttgagttag
      301 taaataagta atagagctaa ctctaaaata aaactattag gaggagatgt aggtgaaaaa
      361 gtttaaaaag aaaatatatt taggattggt tgttggggtg agtatgtttg ggttcatggc
      421 tcatactaaa caagtaactg cagatactaa ccaaaaagaa aggattatga gccaattaga
      481 tgactctcaa atagagtatg tacccaatga gctaattgtc agtttaaagg ctacacaaag
      541 aatttcaaca gagaaaaata aaccgcaaaa tttattgggt gtaaaagtta tggtggaaaa
      601 agatttgact cttaggacaa atgaaagcga ttataataag cgagatcaag tattattagt
      661 gaaattgaac gaagaaatta gtgtggaaga ggcagtaaga acatttaatg cttctgatat
      721 ggtcaattat gcagagccaa actatgtggt tgaactagag ccgttgaatg atgttgagga
      781 gccaaataca actgattctt taggaaatcg atcaattatt cttaacgatc ctaattggtg
      841 taaacaagct aatttaagag agattagcat gccatccgct tgggaagaaa ccaagggttc
      901 taaggatgtg aaggtagcgg tcattgattc aggaattgat tatcaacatg aagatctttc
      961 tggaaatgta gatgaaagtt tgggatacga tttttttgac gatgatacag atcctatgga
     1021 tactatggga catggacaca tattgccggg attattggtg ctcaaacaaa caacgaatta
     1081 ggtgttgctg gaataaattg ggatgttacg atggtaccta tacgtgtatt tggtagtagt
     1141 ggaggtacca gtttggcaat cttaactgaa gctattaatt atgcatccca aaaagggttt
     1201 ccaattatca attttagtat aggtagtgtt gtttatagca gtacagaaaa agcaattaaa
     1261 aactatcctg gattattcgt agcagcagct ggaaataatg ggatagatat gactgataaa
     1321 cctttttacc cagcttcttt ggatatacct aatattatta ctgtaggaaa ttctagtggt
     1381 tatgaacgaa gttctgattc aaattatagg aaaacaggtg ttgatttatt cgctagaggg
     1441 tttcgtgttt ttagtacatt tctaggaagt aaatacacgt atatgtcagg tacctcgatg
     1501 gcaacacctc atgtcactgg tagtgctgca ttagcgttgt cactagatcc tgatctatct
     1561 actgatgaat taaaaaaaat cattttaaat agtgttgatt cattttatga actatctgat
     1621 ttatgtctta cagggggaag actaaatacg aataatgtcg tacgacaagt aacgggtaaa
     1681 cttactgcat ctaatatcac attaagatta aatagtgaat ggtcagatga aattgctaaa
     1741 gagatgtgtc acgttcactg gattgaccag tcaggaaatg atttgacgga tcaagtggtt
     1801 gttctaaaaa acgaagtgga tacatctaac tttggcgatt acgaagtagt ttttgcatca
     1861 cctgatttaa cagatcaaat ctcagttaca gtgaccgtac ctcgattacg taaacttacg
     1921 ctaggaaaag aaacagcaac cattgatatt ggttccgcat gggatagtgt catagccatt
     1981 gatacttttc aagtgaaagc tatagatgat tttgggaatg atgttacaaa tcaagtacag
     2041 gttactatta atgctgatat agatacaatt aattcaattc aagcagtgat taagtttgaa
     2101 gttccagaat taggcttagc ccaaattggt agactgaata ttactgcaaa aaatagtaca
     2161 atcaatggat tgaatgaagt tagcatcgag
//
LOCUS       DASHRQ010000156         2123 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.167, whole genome shotgun sequence.
ACCESSION   DASHRQ010000156 DASHRQ010000000
VERSION     DASHRQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.167"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            253..1551
                     /locus_tag="U2M88_002866"
     CDS             253..1551
                     /locus_tag="U2M88_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEM8375179.1"
                     /translation="MKHTHKIILAVTSVCTVIILGVSIFFVTQAATNNKLENTSQTTQ
                     HSSSSSKPVEDKQTTKQLDQAKQLAASYHYDEAIALLEKDDAKEAQQLLATLKKEKES
                     LVKWEDPTKISHVFFHSLIVDPAKAFHTQQAQGYKDYMVTISEFNKTIDQLYKNNYVL
                     VNLNGLVKKGTDGKLTFTGVSLPEGKKPLILSQDDVSYYEYMDNSGFPSKLIVDKQNQ
                     IKNIYIDNKKETVGDYDMVPLIDSFIKKHPDFSYQGAKGTLALTGYNGVLGYRTSKSE
                     YGDNEKTHKEIEAAKKVADQLKEDGWSFASHTWGHLNMTQASLADIQQDNERWQNEVA
                     PILGKTNILIYPFGADISDWQPYSEANQKFAYLKQQGFDIFCNVDASTPAWGQLGTDY
                     YRNARINIDGIRFEADLKGENPILDHFINVKEMYDQKDRG"
BASE COUNT          759 a          371 c          383 g          610 t
ORIGIN      
        1 ttttagtttt tcttggaaat aaaaacagcg aaaatccgaa cattttcacc tctctcactt
       61 tataactttt acaaaatagt acttctcata aaaagcgctc tagttaaaga aaataagaga
      121 aaaatcaaaa aaaacaaaag tttcttttac acttgtttct atattcgtta caatggttat
      181 gggaagtttt taaaaaggtt aagaaaagct tacgggggtt tttgaaagca aaaagtagta
      241 ggggacgata aaatgaaaca tacacacaaa atcatattgg ctgtcacttc tgtatgcact
      301 gttattattc taggtgtaag tatctttttt gttacacaag cagcaacaaa caataaatta
      361 gaaaacacta gtcaaacaac tcaacactca agcagcagct caaaacctgt tgaagataaa
      421 caaacgacga aacagcttga tcaagcaaaa cagcttgctg caagttatca ttatgatgaa
      481 gccatcgcct tacttgaaaa agacgacgcc aaagaagcgc aacaattgtt agcaactttg
      541 aaaaaagaaa aagaatcctt ggtaaaatgg gaagacccga ccaaaatctc acacgtcttc
      601 tttcatagtc tgatcgttga tccagctaag gcttttcata cccagcaagc acaagggtat
      661 aaagattata tggtcactat ttccgaattc aataaaacca ttgatcagtt atataaaaat
      721 aactatgttc ttgttaatct aaatggatta gtcaaaaaag gaacagatgg aaaactgacc
      781 tttacaggtg tttctcttcc agaaggaaag aaaccgttaa ttctttcgca agacgatgtc
      841 agctattatg aatacatgga caattcagga tttccaagta agttgattgt cgataaacag
      901 aaccaaatca agaatattta tatcgacaat aagaaggaaa ctgttggtga ctatgatatg
      961 gttccattga tcgattcatt cattaaaaaa catcccgatt tttcttatca aggcgccaaa
     1021 ggaacattag ctttaacagg ctataatggt gtattaggtt accgtacatc aaaatcagaa
     1081 tatggtgata acgaaaaaac acacaaagaa atcgaagcag ccaaaaaagt tgctgatcaa
     1141 ttaaaggaag atggttggag ttttgcttct catacttggg gacacttgaa tatgacacaa
     1201 gcttctttag ccgatatcca acaagataat gaacgttggc aaaatgaagt tgcacctatt
     1261 ttaggcaaaa caaatatttt gatctaccca tttggcgctg acatcagcga ttggcaacct
     1321 tattctgaag caaatcaaaa atttgcttac ttgaaacaac aaggatttga tatcttctgc
     1381 aatgtcgatg cctcaactcc tgcatgggga caactaggaa ctgactacta ccgtaacgcc
     1441 agaatcaata ttgacggtat ccgtttcgaa gctgacctaa aaggagaaaa tccaatactt
     1501 gatcacttta tcaacgtcaa agaaatgtat gaccaaaaag atcgaggata gaggttgtga
     1561 aagaagcgtc cagctccgag caaataagaa ctagatttct aaaatagttt ctcatatttt
     1621 ggaaatctag cgcttattgc tgaggagttg cttcttgaac accgtgaata gaggttgtaa
     1681 aaagagcgtt ttgacttatt gttgaaggtc agctctttga acaccgcgtg ttaggttgtg
     1741 aaaaggctgg tctgcatcaa gcaataagaa tgaacagaga aaaacagctt cttgaatacc
     1801 gcagataggt tgtaaaaaaa ccgttttgac ctgacgataa ccagtaaaac agaggtgaga
     1861 ccattgctaa aagtagcagt ggtctcatct ctttttttgc acaagtcata tttgattgtt
     1921 tcgttatttt aatcaggata aagttctttt tttcatgcta aagagtgatt ctttcttctc
     1981 gattttcact ttcttcgttt aacttttggt aaatacttct gtcagaaaaa gctctaggta
     2041 gacacacgaa agcagataaa aagcctaact tttgcaacag tttatgctgt cgttcaaagt
     2101 taggctttta cagtttataa ata
//
LOCUS       DASHRQ010000157         2107 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.168, whole genome shotgun sequence.
ACCESSION   DASHRQ010000157 DASHRQ010000000
VERSION     DASHRQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.168"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(208..303)
                     /locus_tag="U2M88_002867"
     CDS             complement(208..303)
                     /locus_tag="U2M88_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEM8375180.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            978..1697
                     /locus_tag="U2M88_002868"
     CDS             978..1697
                     /locus_tag="U2M88_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEM8375181.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            complement(1834..>2107)
                     /locus_tag="U2M88_002869"
     CDS             complement(1834..>2107)
                     /locus_tag="U2M88_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375182.1"
                     /translation="NTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNS
                     KVYQEVKEKIEAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
BASE COUNT          662 a          336 c          404 g          705 t
ORIGIN      
        1 aatacgagct ctcagagcga ctttatttgt aaatgttgca tataagaaat aattacggat
       61 tttagacttt tgtaggatat ttttgaaata aatatttatt tctgttataa tttgattgca
      121 aataagtgaa cacgaaacac acgaaagccc cagagaggtc ggattctctg gggctttttt
      181 tgtgggctga gtcgccttaa ttaagtttta cttcttgttt cggttattca accagtaact
      241 gaacaaagcg cttaaaagcc caacaagaag ggagcaaata aagttagtga acacgaaaca
      301 cacctccttc cagtcgcaag actgtcggat aggcgacaat caattataca ataaaaaagc
      361 aaaaataagc ctaagttttt tattgccttg gggttgttag ggcttgccct gaccgcctgt
      421 aaggcgcttt agaacgaagg acatagtttt tatcgaatct tgtcttagaa cggcttagac
      481 gagctctcag gacgccacaa aggagtccct aatgaattat ttttggcgaa ggagcctaaa
      541 ggcgactgct gcaagtaaaa tgtgagagcg tagcgatcac tccttcttac agcgaccata
      601 gggagctaga ttggcagttt aatatcatgc gcgaacctgt ttattttaaa aagtgggtga
      661 ttttttggcg ggaaattttg ctcgcgcgcg cgcgtttatt ttatttattt tttaaagatt
      721 ttttagaacc ctttttaggg ggcagttgag ccttactctc ccaacggata cagaagggta
      781 ctaacaactt tttatctgag tactaacaac tttttatctg agtactaaca actttttatc
      841 tgagtactaa caacttttta tctgagtact aacaactttt tatctgagta ctaatatcct
      901 tttgtataat aacaaccaac ctatttacga gttggttgtt aaaggttatt atttatttgt
      961 aaggggggaa gaaaggaatg gatgaaaaaa cggaattaac agtcaaatat caaaatgaat
     1021 tgaatttagt accgctgaaa aatttcaatg ctaagcagat ggatttattt tttgcattat
     1081 gcgctcgtat gaaagatcgt ggtttgaaaa aagttagatt ttctttcgag gaattaaaag
     1141 agttaagtga ttataagatg actgctacaa aagcctttgt cggtgattta gaaaagctct
     1201 ataaagatat gcttaatctt acttaccgta ctgaagatga ggattctatt cgctactttg
     1261 ttttgtttac tggttttgaa atcaataaaa caaaaaagtt tgttgaagtc agtgttaatc
     1321 cggacctaga atatattatt aatggtttaa cagctgaatt tagtagattt gaattatctg
     1381 ctttcacgtc gttgcgttct acgtatgcaa aaactctatt ccgactgctt atgcagtacc
     1441 gttctactgg ttattatgta gttcgtattg aagagtttag gagcttaatg gatatcccag
     1501 aatactatca aatgggtaac attgatcaaa aagtattaaa tcctgcatta cgagaactaa
     1561 aaaaatactt caatgatctt aaagtcacaa aaataaaagc aaaaaagggc aataaaatcg
     1621 ccaaattaga atttactttt tcaagcctaa cgtcagattt gccaaagatt tctctgcgta
     1681 actggttaga agaataaata aaaaagaact cgcccccctt acattaagca agtagacaag
     1741 tcccttacaa ccgactaaga ttgctagtac attctagcat attgtatggg gcttctaaat
     1801 aaaaaacaga taggaataac tcctatctgt tttttatcgt tttagaaaat ttgttgggtc
     1861 gaaatttcta gaagtgactg tttgactggt ttctttttga tgattctttg gtcgatttcg
     1921 atttgatgga tttagtttag cttcgatttt ttctttcact tcttgatata ctttagaatt
     1981 tgtgccaata agaacgcctg tatcttttag atataaatca gcaaattctg cgatgatttc
     2041 agtcatgatg gaaatttctt ttcccatagc atttagtttt gcctgattgc tggtagtggt
     2101 cgtgttg
//
LOCUS       DASHRQ010000158         1946 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.169, whole genome shotgun sequence.
ACCESSION   DASHRQ010000158 DASHRQ010000000
VERSION     DASHRQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.169"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            178..480
                     /locus_tag="U2M88_002870"
     CDS             178..480
                     /locus_tag="U2M88_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375183.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(996..1069)
                     /locus_tag="U2M88_002871"
     tRNA            complement(996..1069)
                     /locus_tag="U2M88_002871"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1033..1035),aa:Ile,seq:cat)
     gene            complement(1624..1938)
                     /locus_tag="U2M88_002872"
     CDS             complement(1624..1938)
                     /locus_tag="U2M88_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375184.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          708 a          290 c          301 g          647 t
ORIGIN      
        1 tataagggtt tgagcatttt ttattttcgc agattttcgt taaatgaatg aaaatggcat
       61 tcaaaatagc attcaaaaag aacacttttt aatatatatg ttccatccat attatcaatg
      121 atattcctac tttacaaaaa gaacattagt tcgtatactc tttttgaggt gaacattatg
      181 ttgatggaag gaaaaacaca attatggttt aaatttgatc cttcgaatag atttgtcaaa
      241 gatttttata aggtgtggga ttcagaagtt ttctttttag caatcgaaga tagcttatta
      301 atcaatctct attactctaa taagaactac tttaaaattc cggcaacaaa aaccagaatg
      361 aagaaagatg tgtatttttt gtttgatatc gtgactgatg tgccagacgc acggagcgat
      421 catcggcgtt atgactattt gaagtatact ttcgttgatc cagaaaggta taaagattaa
      481 agtaggctac ctaaaaaagg tagcccggaa cggattttat caccatactt ataaaaggga
      541 tattttttaa gttagtatta agattgtgta atatgatgat atctatattt tatagtatca
      601 gtgctataaa atcaaaaata agtcactaat taactaccac tccaattata agtctttttc
      661 ccattatttt ttacagttat atggtatgct ttttaatggc ttcaaatata aaagagttta
      721 aagcgtaaca cacttatggg gaagtggttt ttggggaacg ctttaaactc ttctttatta
      781 ttatctcaca atataaccca aatgtctttc catttaaaaa tcaaagtaaa acttttcaaa
      841 tatacagaag tataactatg tgaaacatcc tttcattaat ccataaaagg atacataaaa
      901 aagccactca tttgagtggc aatgaagaaa agctttagct tgtataatac tcttcaaaaa
      961 attctaacac agaacgattc aattggctac attaatgtac cctgtaggac tcgaacctac
     1021 gaccggacgg ttatgagccg tctgctctga ccaactgagc taagggtagt ggttgttgcc
     1081 acataaagcc ataaacaatc aaccagtaga atgcgtggca acaaacctgt tatcgcatat
     1141 cttggagtgt gactatttat gagtgatagt gaagatatgc gtctccatat atgtatggtt
     1201 gagacagata aacatgtatg aaaaacgttg ttaaatcaac gtttttttat tttggtcaag
     1261 gagagaaaag tagagaaata caaaattttt tgcattgatt ttgcattcaa aaaaacattc
     1321 aaacatttag aaatccccaa tacgtattca ggaaaatcta ttttaaggtt tacaaaaaga
     1381 acttttgttc gtacactctt tttgaggtga acttttatgc tgatggaagg aaaaacacaa
     1441 ttatggttta agcatgttcc ttcaaataag ttttttaatt ataaactcta gagaaattaa
     1501 ttcatcctaa cataaaataa ttgttcactc aaaagttttt ctagaacaat taaaacagca
     1561 ggcttcacat tcgctataca tttgtaagaa gtaataattc ctgtactaaa aggcataatt
     1621 gctttaatta ttttttgact tctcttcaaa cacaagaagt agttgtgtaa taattgcaga
     1681 aataaggatt ccaataatag atataaaatt tgacatggaa acgtctataa cattaaaaga
     1741 ataaagtgtt gaagaaagta aactgattga aataacccct ccgtaaaaaa tagtctgtga
     1801 atatacccac tttttttcag aagacgtact tcgtttggtt ctaaaaccta tccctgcatt
     1861 tctactctta tcaaaaaacc aaatatttag aagtaataat attgctatta ttaagataaa
     1921 attactgtaa aaatccaaaa gctatc
//
LOCUS       DASHRQ010000159         1933 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.170, whole genome shotgun sequence.
ACCESSION   DASHRQ010000159 DASHRQ010000000
VERSION     DASHRQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.170"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            203..1168
                     /locus_tag="U2M88_002873"
     CDS             203..1168
                     /locus_tag="U2M88_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEM8375185.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVDDKVHPLKLSWSIDRITIVG
                     KLKENIYYHTPNDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLEMQ
                     LRRGKATDWHAMVRESLDSFASPHYLPGDVKPVDRIMIKGLNQDHSEWAYISRNLKYR
                     LRKLLKEESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEE"
     gene            1368..1571
                     /locus_tag="U2M88_002874"
     CDS             1368..1571
                     /locus_tag="U2M88_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375186.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFIGDSAKEGFNLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
BASE COUNT          651 a          284 c          438 g          560 t
ORIGIN      
        1 aaaaaaatag tcgagcagtg ttgacgcact ctcgaccttt acggaaacta atcacactag
       61 ctccgtaact tcttaatcat tatacgaata tttttaggtt tcttcaagac tagagatttt
      121 ttggtaatga atttttaaga gttacgtgaa cttttgaaaa ttctttccag aagtctcttt
      181 tttctttgag gaggaactaa atttgagtaa aaaaggcacg atcataaatg agtgggaaaa
      241 attaacagcc ccctctaaca ggcggttaag tgttgacgat aaggtacacc ccctaaaatt
      301 aagttggtca atcgatcgaa ttacaatcgt cggaaaactg aaagaaaata tttattatca
      361 cactccaaat gatgtgttga ttttagattt tgaacagctt atgcgcctaa atgaaggtaa
      421 cggttattta agatctgtta gtaataatgg ttggcagttg ttagaccaac atgaagagaa
      481 tattgcttat attgaaattt taaaatggca agaggggaaa gggcgtatag acttcaatcc
      541 gaataagatt agtcaattct tagctagttc catgaaaaat tttattcatg atttgtttat
      601 cgaacctcac ttttcacgtg cagatattgc ttgcgacatt atcgatgtac ctgatgagtt
      661 tattacacaa tatcgggtag ttgatccagt ttcttttaag cctatttatg gacggaatgg
      721 aaaattagag acggcatatt ggggatctcg ctcttctgaa cgtcaaattc ggatgtataa
      781 taaaaaactg gaacaagaga agaaaagaaa aattgtgcca aaagaaatcg tgagttggtg
      841 gcggttagaa atgcagctaa gacgtggcaa agcaacagat tggcatgcga tggttcggga
      901 aagtttagat agctttgcaa gtcctcatta cttgcctgga gatgtaaaac ctgttgatag
      961 aattatgata aaagggctga atcaagatca ttcagagtgg gcgtatatta gcagaaattt
     1021 gaaatataga ttgcgcaaat tgttgaaaga agaaagccaa aatgatgaat tgacgaatca
     1081 tttgcgagaa acctttgcag aatcggcaga cgatttaaaa aaagaattag acacatggct
     1141 tctagggcta gacgtaacag aggagtgata ccgaataaga aaagtcagat gagtgagagt
     1201 aaatttacgt caatgtttaa tgacgtgagt ttacgataag cgattatttt gcgtgaagca
     1261 aaataaagcg taaaaggagt tgagagcgtg acacgctttc ggctcctttt ttcgttgtca
     1321 cttgtgacag cgaataccct cggagagcat ttggaattgt gacagccatg tcacaattag
     1381 gaaaatgtta ctctttgtca aagagtaaca gaaaggttta cactcttttt attggcgaca
     1441 gcgcgaagga ggggtttaac ttggggttta gtatttcgtt caaaaaggga acaaaaaata
     1501 caagcatttc tcataataat cgtgagttaa ctgatagcca aaaaatcaat ggtttgtctg
     1561 gtagaaaata attagcagat ttacaagtgg gaaataaaaa gctgaaagaa gaaaattgca
     1621 tttcaaacta cattttctac aatagctcta aaatggcata ttttgcgttc tgagcggttt
     1681 tagcgtttat ttcgtttagt tatcggcaca atcggtgaaa cacgttagag gagcgaaaat
     1741 ggagcctgta aggcgactgg tagcggaacg gtcgtgttag attgtgtagc cgatagctga
     1801 acgaaataaa tgctggcagc gccattctat ttcggcagga ggaggctcaa ggggttcggg
     1861 gaatgaaatt ccctgaggtc tttttatttt ttctagagga gtgctataat caaagaaaat
     1921 gagaaaaaag agt
//
LOCUS       DASHRQ010000160         1744 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.171, whole genome shotgun sequence.
ACCESSION   DASHRQ010000160 DASHRQ010000000
VERSION     DASHRQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.171"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            131..505
                     /locus_tag="U2M88_002875"
     CDS             131..505
                     /locus_tag="U2M88_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEM8375187.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="U2M88_002876"
     CDS             complement(619..1248)
                     /locus_tag="U2M88_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEM8375188.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="U2M88_002877"
     tRNA            complement(1382..1454)
                     /locus_tag="U2M88_002877"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="U2M88_002878"
     tRNA            1668..1741
                     /locus_tag="U2M88_002878"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
BASE COUNT          602 a          256 c          306 g          580 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaa
//
LOCUS       DASHRQ010000161         1554 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.172, whole genome shotgun sequence.
ACCESSION   DASHRQ010000161 DASHRQ010000000
VERSION     DASHRQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.172"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            3..968
                     /locus_tag="U2M88_002879"
     CDS             3..968
                     /locus_tag="U2M88_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEM8375189.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          501 a          245 c          365 g          443 t
ORIGIN      
        1 atttgagtaa acgtggcacg atcataaatg agtgggaaaa tttaacagcc ctatctaaca
       61 ggcggttaag tgtgcaaact ttgaccaccc cccttaaaat acagtggtca attgaccgaa
      121 ttaccattgt tggaaaactc aaagagaata tttattatca cacgcaaaat gatgtattga
      181 ttttagactt tgaacagtta atgcgactga atgaaaataa tggttatgtt aaatctgttg
      241 gtctaaatgg ttggcaaatt gtggataagc atgatgagaa tattgcttac attgaaattt
      301 tgaaatatca agaagggcaa gggcgaattg actttaaccc aaataaaatt agccaatttt
      361 tggcgggttc gatgaagaac tttatccatg atttattttt agaaccacat ttctcacgtg
      421 ctgatattgc ttgcgacatt atcgatgtgc ctgatgattt tattacgcaa tatcgtgtgg
      481 ttgaccctgt ttcattcaaa ccaatctatg ggcggtctgg gaagctggaa acggcatatt
      541 ggggtagtcg tgccagtgag cgacaaataa ggatgtacaa caaaaaattg gaacaagaaa
      601 ccaaacggaa aattgtacca ccggaaataa aaacttggtg gcgactggaa ttgcagttac
      661 ggcgtggaaa agctactgat tggtatgcga tggttcatga aagtttagac agtttcgcaa
      721 gtcctcattt tttgccgact gatacaaagc atactgatag aattatgatt gcaggattga
      781 tttcagatca aaatttatgg gctggcttgg ctcgtcatac aaaatataaa tatcgaaatt
      841 tgttgaaaaa agaaagccaa agtgatgagt tagttaatca tttacgagag acattttcgg
      901 aatcggcaga tgttttgaaa aaagaattgg atacgtggct tttggggttg gacgtgacgg
      961 aagaatgagt ggatgaattt ttgtccactt ttttattttc agcacaagtc ggcatggtgg
     1021 gtcgtgcgtt tgcagcaaga gcgaaaaaac gctaacgaat ccgcaaatgc cgacttggat
     1081 aaaacagttg cgcaaagaga cttggctttt tataataaat ttagggatat cccagtgtca
     1141 ctctttgggg taattcgggg gaaggagtta cccctcactc tggaaaatta gagcgaagca
     1201 gacgagatta tcccaatgaa tatgacggct tactcggagt gataccgagt aaaaaagtca
     1261 gataagtgag agtaaattta cgtcaatgtt taagataagc gattattttg cgtgaagcaa
     1321 aataaagcgt aaaaggagtt gagagcgtga cacgctttcg gctccttttt tcgttgtcac
     1381 ttgtgacagc gaaaaccctc ggagagcgtt tggaattgtg acagccatgt cacaattagg
     1441 aaaatgttac tctttgtcaa agagtaacag aaaggtttac actcttttta ttagcgacag
     1501 cgcgaaggag gggttcacct tagggtttag tatttcgttc aaaaagggaa caaa
//
LOCUS       DASHRQ010000162         1519 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.174, whole genome shotgun sequence.
ACCESSION   DASHRQ010000162 DASHRQ010000000
VERSION     DASHRQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.174"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(329..628)
                     /locus_tag="U2M88_002880"
     CDS             complement(329..628)
                     /locus_tag="U2M88_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375190.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(634..864)
                     /locus_tag="U2M88_002881"
     CDS             complement(634..864)
                     /locus_tag="U2M88_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375191.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(1114..1314)
                     /locus_tag="U2M88_002882"
     CDS             complement(1114..1314)
                     /locus_tag="U2M88_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEM8375192.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          539 a          244 c          223 g          513 t
ORIGIN      
        1 aaagacattt gggttatatt gtgagataat aataaagaag agtttaaagc gttccccaaa
       61 aaccactgcc ccataagtgt gttacgcttt aaactctttt atatttgaag ccattaaaaa
      121 gcataccata tttttgaaaa aaagtgagaa aaaaggctta taattggagt ggtagttaat
      181 tagtgactta tttttgattt tatagcactg atactataaa atatagatat catcatatta
      241 cacaatctta atactaactt aaaaaatatc tcctttcata agtatggtga taaaatccgt
      301 tccgggctac ctttttaggt agcctacttt aatctttgta tctttctgga tcaacgaaag
      361 tatactttat atagtcataa cgccgatgat cgcttcgagc gtctagcaca tcagtcacga
      421 tatcaaacaa aaaatatacg tctttcttca ttctagtttt cgcagcaggg attttaaagt
      481 agttcttatt agaatagtag agattaatta ataagctatc ttcgattgct aaaaagaaaa
      541 cttctgaatc ccacacctta taaaaatctt tgacaaatct attcgaagga tcaaatttaa
      601 accataattg cgtctcatta aaaagcataa ccattactcc gatctatttt taaacttagt
      661 ctctacctct aatatatatc gagtttttat tttgccttca gagaatactg tttctttttt
      721 tgcagttaca ggttgtttat tttcggaaaa agctaatata gctaaaattg aaacatccat
      781 ctggaatttg tctttttcgt tgctttgctc ataaaaatct gcatattcat cactaatatt
      841 ttttctaata aattcttcca tcataaagat cacctcgaaa agagtatacg aacaaacgtt
      901 ctttttgtaa aggtggaatt tattatacta aataaatagg tgaacaagta cttgtgccaa
      961 attatgtgcc aaaaaaaatc gaatttaata aaacacaaac aaaaagaaat cctattatga
     1021 taacatttct tataataaca aacacaacag aagacgtgta atagttagtc aggaacgtac
     1081 aaataacccc tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt
     1141 ttctaaactg ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag
     1201 taaaatagat gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga
     1261 aaaattgatt actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca
     1321 atagattcga attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata
     1381 tcgttcttaa ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg
     1441 gagatgactt tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt
     1501 tcaaagtacc atataaaaa
//
LOCUS       DASHRQ010000163         1519 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.175, whole genome shotgun sequence.
ACCESSION   DASHRQ010000163 DASHRQ010000000
VERSION     DASHRQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.175"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(216..1127)
                     /locus_tag="U2M88_002883"
     CDS             complement(216..1127)
                     /locus_tag="U2M88_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HEM8375193.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          452 a          311 c          224 g          532 t
ORIGIN      
        1 cactaaccag attagtgtca ctaaatataa agctagcaat cctctagata atatttttcc
       61 caatataatt tcctctaatt cttttgttta agttgtatca ttagtatagc aatgtcctag
      121 ttaacgtggg ttttaaatta aaaagctaac gttaccccaa aagtgctagt acaataatgc
      181 tgttcattaa tttcgttggt ttcattccgc catccttacc tccttgaatt agtatgtgtt
      241 gatccgggaa acactgtcac aattacaccg tctatattgt ttcctgatat ctcataacga
      301 agattagtcg gcacatgaat ggtggtattt ttagtaacag gataataaaa gatctcatat
      361 ttttccccat ttacactaac agaaatggtt ttttcttctt ttaggtaatc catatattcc
      421 tcgagaacga aattcttttc tttcataatc gcactatgtg gtaaaccaac atagcgaata
      481 tgccatggtt catattgaat tcctgttaac tctgttttgt cctctggata acgtaaaatg
      541 aacccgtatt tccaagcatt ttcttctatc cactttcctt caggggctcg ttccattttc
      601 gtcaagcttg atcctacatc tagtgataaa cctgaattat gctcactata acctgctggt
      661 aaggcatact cagcccccat ttcttggtaa agcacacttt gctcatcaaa gtctcgatag
      721 ccactattaa taataaaatg actaacgcca ccctttacag catcattgac catctctgaa
      781 aatttttgtg ctatttcttt tgacatataa atattactat caagcaaccc gtatccattt
      841 attaattcgt catgtttaga taaattcacg atatctgact tcacactttc ttggcgaaca
      901 ggatatttac tattgattaa tagcagattt ccttgataaa cctgttcttc tgtaattgtt
      961 ttctcttggg tattttcaga agtcccacta ttttctaaat gttctttggg attttgatca
     1021 taattttgaa attcgacttt ttcctgagaa aacagtgctt cattaacgta gtcataacct
     1081 aagtatatta agaataacaa taacaataaa aaaaacaact tcttcatttt cagtctcctc
     1141 cttaactata tagttggaag tatgaaatat ttctagaatc ttccccttaa acggcacaat
     1201 ctcgtttgag attgtgccgt tttgccccgt aaattacagt caattatttc gccctactgg
     1261 atttactatt atcaccaatg tagatgaatt ttcataagtg atacccatgc tgaatttaac
     1321 gaggctgaac tgcctgttga caatgggcgg tgtgaaatac cacccctcta tgcgatcagt
     1381 cttattatca ggctatcttg ccgcagcgca cgcagataat gaccgcaata tcgctctgcc
     1441 ttacatcttc aatggttctg ttgcaaagtt ttaaatctac tatcaaataa ggtagaataa
     1501 tagaaaaaga tagcaggag
//
LOCUS       DASHRQ010000164         1457 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.176, whole genome shotgun sequence.
ACCESSION   DASHRQ010000164 DASHRQ010000000
VERSION     DASHRQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.176"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            109..1041
                     /locus_tag="U2M88_002884"
     CDS             109..1041
                     /locus_tag="U2M88_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEM8375194.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1113..>1457
                     /locus_tag="U2M88_002885"
     CDS             1113..>1457
                     /locus_tag="U2M88_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375195.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          228 g          420 t
ORIGIN      
        1 gatgaaataa atcaaaagta tgatgaaaca ttaaaaaatg tctattcttc acttatcgtt
       61 tcaaatatag aagaaacttc atcttctgaa ataaaaaata agctttcttt gttaaaatta
      121 aaagaaaagg aatatattat tgacgatgct gtttctgtcc ctcttatttt aaataaacga
      181 gagaaaaata atttaaagaa aaaaatgctc attccatttg attcagaagt gaaaggatta
      241 ctcaataaac taactatttc taatatagac tcgactaggg aaaaaatcat aaaaatattt
      301 gataaaatta ataatttatt caagaatgac aatgctgaat taaggaaggg tcttttagag
      361 tttaaacttc aagaactaga acttcattac tcttatttag tgaaaattac cgaagaaaaa
      421 gaacaacaaa aagcaataaa agaacagatg atagaagagg aaaaagttcg tagagaaatt
      481 gatcgtgaaa agaaacgtat tgataaagaa caacgtcaat tcaattctga aatttccaaa
      541 ttaatcgcat atatgcaaaa agctaatgca gatgtggaaa aagaattata tgcaaataaa
      601 attcaagaac tggaagaaaa attaaaagaa ttagaagtta tacgtgaaaa tgtattacaa
      661 agagaattaa atactagagc cggttatgtg tatgtaatta gtaatattgg gtcttttggt
      721 gaagacatat ataaaattgg tatgactaga agattagaac ctatggatag aataaaagaa
      781 ttgagtagtg catcggttcc atttgaattt gacgttcatg cgatgatttt ttcagaggac
      841 gctccgtctt tggaaactaa attgcataat cattttagaa aacaagaagt taataaaatt
      901 aatcaaagaa aagaattttt caaagtttca ctagacgaaa tagagaaagt tgttttagaa
      961 aattataatg gaacagtaac atttacaaaa ttagctaaag ctgaacaata tagacgatct
     1021 ttagaattgt caaaagatta aatatataaa aagccttcgg gctttttctt ttaacaatta
     1081 ataggaacgt atattcgaaa ggaatagaaa taatgtggtt agaaaaaata aatgacaaaa
     1141 aatttaagta caccgaacgt tatacggatc ccttaactga aaaaaagaga aaagtgtccg
     1201 ttactttatc tagcaattca cgccaagctt ggaatcaagc taacttactt ttgaatgaaa
     1261 aaatcgcaga aaaaataaaa cgaaatgaag aattaccact aacttttgga gaattaaaaa
     1321 ctaaatggga tgaaaaatac aagccaactg taaaagaaag ttcctatcga acaactcagg
     1381 tttatctatc attaatttca aaatatataa aagatgatgt acttgtaaaa aacgttaata
     1441 gtaatcttat acaagat
//
LOCUS       DASHRQ010000165         1431 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.177, whole genome shotgun sequence.
ACCESSION   DASHRQ010000165 DASHRQ010000000
VERSION     DASHRQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.177"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            98..1390
                     /locus_tag="U2M88_002886"
     CDS             98..1390
                     /locus_tag="U2M88_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEM8375196.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          466 a          293 c          257 g          415 t
ORIGIN      
        1 acaccagcat cttttttatt ttccaaaaca tatagaaaag acaccttgaa atcctttaga
       61 attaatttgg cgaaccaacc caaaaggagg aatcaagatg tcttacactc accttatcaa
      121 agaaacatta gatattctag acttatcagt tacttttaac gaaaattgtc tcacaaaaga
      181 aaaatacaaa gggcaaatct gtcatattta tcgtggaaac ttgatttata cagcccaaga
      241 atgcatccac tgtaaacacc agattgcttc agatattgtg cgttggggta caacaaccgt
      301 ccggttgtta atgaacgatg tttctgaata ccgtacctac ctcgaattga aaaagcaacg
      361 cttcaaatgc aaagcttgtc agcggacctt tgtcgcagat acttctgttg cgaaaaaaca
      421 ttgtttcatc agtgagaaag tccgctggtc tgtcgtgact cgtctgaaaa aaaatacctc
      481 aatgacagag atcgccgctc agaaaaatct atcggtctct tctgtttacc gtattatgaa
      541 gcgattttat cgtccgttga atccttttcg gacaccgtta cctaaggtac tttgttttga
      601 tgaattcaaa tcagttcggg gtgtttcagg tgctatgagt ttcatcatga tggatggaca
      661 aacacagcgc ttattggaca ttgtcgagaa ccgacaactg ccttttttaa aacgttattt
      721 tagtcatttt tcacgagaaa ttcgtgaagc ggtcgaatgg atcgtgattg atatgtacgc
      781 tccttatgta tcgctcgtta aaaagctgtt tccaaaagca cagctgatta ttgaccggtt
      841 tcacatcgtc caacatattg gtcgtacctt tcgcaatcat cgaatcaaag aaacgaacca
      901 actattaaaa agcaaagaac aaaaacacta tcaattaggt aagcaattaa aaagatactg
      961 gaaacttctt caaaaagatg aacgcaagct tgattatact cgacgccttt ggcgacctgg
     1021 cttcaaagct catttaacag agaccgatat tgtggatcgc ttactgaaag ggagccctgc
     1081 tttgcgtgta ggctatcagt tatatcaaga ctttctttac gctgtaaagg aacgagacta
     1141 cgtatcgttt gaagaactac ttaccaataa tatcatgctt cccgaaggct atcagacgac
     1201 cttgagaacg ttccagaaat ttctgccaca aatcaaaaat gctttacaac aaagttattc
     1261 aaatggccca cttgaatgtc tgaacaacca tatcaaagtg ttaaaacgaa atgcatatgg
     1321 ctttcgtagc ttttataatt tcaaattgcg catcatgatt cgtcatggca acgcattgat
     1381 tttcaactaa aaaagatcaa gaggaactga tccacttgat ctccaattat t
//
LOCUS       DASHRQ010000166         1424 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.178, whole genome shotgun sequence.
ACCESSION   DASHRQ010000166 DASHRQ010000000
VERSION     DASHRQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.178"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(150..1337)
                     /locus_tag="U2M88_002887"
     CDS             complement(150..1337)
                     /locus_tag="U2M88_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEM8375197.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DASHRQ010000167         1389 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.179, whole genome shotgun sequence.
ACCESSION   DASHRQ010000167 DASHRQ010000000
VERSION     DASHRQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.179"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            278..820
                     /locus_tag="U2M88_002888"
     CDS             278..820
                     /locus_tag="U2M88_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375198.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..1326
                     /locus_tag="U2M88_002889"
                     /pseudo
     CDS             976..1326
                     /locus_tag="U2M88_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414636.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          551 a          164 c          264 g          410 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc ttgatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccagatggaa tggcaacact ttttttgaca aaaattataa ggtgttgtga atgctgcggt
     1381 gttatggct
//
LOCUS       DASHRQ010000168         1382 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.180, whole genome shotgun sequence.
ACCESSION   DASHRQ010000168 DASHRQ010000000
VERSION     DASHRQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.180"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            94..>1382
                     /locus_tag="U2M88_002890"
     CDS             94..>1382
                     /locus_tag="U2M88_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEM8375199.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLT"
BASE COUNT          436 a          274 c          260 g          412 t
ORIGIN      
        1 tcaccaacat cttttttgta taaaaaaata tagaaaagac accctgaaat cctttagaat
       61 taatttggct accaattcat aggaggattc aagatgtctc atactcagct tatcaaagat
      121 accctaaata ttctagacct aaatatctat ttcgaggaaa attgtttaac taaagaaaaa
      181 tacaaagggc aaatttgctt gatttacaaa ggaactcttc tgtacaagcc agaagagtgt
      241 tcccattgtc tttgtgtcgt cccctctagg atcattcgtt gggggacgac aaccgttcgg
      301 ttgttactga atgatgtgtc tgagtatcgc acttatctgg agctgaagaa acaacgattt
      361 aaatgtaagt cttgccaaag aacattcgta gcggatacct ctgttgctga aaaacactgt
      421 ttcattagtc aaaaagttcg ttggtctgtc attgctcgct taaaagagaa tacttccatg
      481 acggaaatcg ctcgtcagaa aaatatttcc acttcttcgg tctatcgtgt catgaagcga
      541 ttctaccgcc cattgaatcc tttcaaacag actttgccaa aggtcttatg ctttgatgaa
      601 ttcaaatcgg ttcgaagtgt tgccagtgca atgagtttta tcatgatgga cggacaaagc
      661 catgcattat tggatattgt cgaaaatcga cgcttgcctt atttagaacg ctatttttct
      721 cggttttcat tagcgactcg tgaagccgtt caattgattg ttattgatat gtatgcgcct
      781 tatgtttcgt tggtgaaaaa gcttttcccg aatgctcagt tgatcatcga tcgtttccac
      841 atcgtccaac atattggcag aactttcttg aatcatcgag tgaaagaaac cacggtacga
      901 cttaaaggaa attcccactc tcaacgaggg ttagggaaaa aattgaagcg ctattggaaa
      961 ctcctacaaa aagaagaagc aaagttaaac tatgaaaaac gggtttggcg tgccagtttt
     1021 aaagattatt tgactgaaag tgagattgcc gatcgactgt tagctgcttg tcccaactta
     1081 cgccaaggct atcagttata ccaagacatt ctttatgcgg taaagaaaag agatcagtca
     1141 ctatttgagg attgtttaac tagagacatt agcggtctgc cagaaaccta tacaaccact
     1201 ttacgaacgt ttaaaaagtt tctgccacag atccaaaatg cgcttcacta cagctattca
     1261 aacggaccat tggaatgctt aaacaaccac atcaaagtat taaaacgcaa tgcgtacggc
     1321 tttcgcaact tttacaattt caaactacga attatgattc gtcatggcaa aacattttta
     1381 ac
//
LOCUS       DASHRQ010000169         1364 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.181, whole genome shotgun sequence.
ACCESSION   DASHRQ010000169 DASHRQ010000000
VERSION     DASHRQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.181"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            82..>1364
                     /locus_tag="U2M88_002891"
     CDS             82..>1364
                     /locus_tag="U2M88_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEM8375200.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DASHRQ010000170         1364 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.182, whole genome shotgun sequence.
ACCESSION   DASHRQ010000170 DASHRQ010000000
VERSION     DASHRQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.182"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            82..>1364
                     /locus_tag="U2M88_002892"
     CDS             82..>1364
                     /locus_tag="U2M88_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HEM8375201.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          463 a          255 c          259 g          387 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
     1081 tatccctatt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacggct ttcgggaaaa gctccaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcgatgatct atccttacac caatggaaaa
     1261 atagaagcga agaataccca tataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DASHRQ010000171         1294 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.183, whole genome shotgun sequence.
ACCESSION   DASHRQ010000171 DASHRQ010000000
VERSION     DASHRQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.183"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1244
                     /locus_tag="U2M88_002893"
     CDS             <1..1244
                     /locus_tag="U2M88_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEM8375202.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DASHRQ010000172         1249 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.184, whole genome shotgun sequence.
ACCESSION   DASHRQ010000172 DASHRQ010000000
VERSION     DASHRQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.184"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>1249
                     /locus_tag="U2M88_002894"
     rRNA            <1..>1249
                     /locus_tag="U2M88_002894"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          327 a          282 c          384 g          256 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccc
//
LOCUS       DASHRQ010000173         1247 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.185, whole genome shotgun sequence.
ACCESSION   DASHRQ010000173 DASHRQ010000000
VERSION     DASHRQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.185"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..813
                     /locus_tag="U2M88_002895"
     CDS             <1..813
                     /locus_tag="U2M88_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEM8375203.1"
                     /translation="GKSTCAQMLGFESAVNEGKRTLIIDLDMQGNTSDVMNLTHMNFT
                     NEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPADMSFEMYDDWIKDQY
                     NDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIADWAIVVLQTQVKSMR
                     NAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQAQEIYGEHLIKNVVL
                     KNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEEHENWYAEVE"
     gene            814..>1247
                     /locus_tag="U2M88_002896"
     CDS             814..>1247
                     /locus_tag="U2M88_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375204.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYP"
BASE COUNT          469 a          186 c          262 g          330 t
ORIGIN      
        1 ggtaaaagta cgtgcgcgca aatgttaggg tttgaatcag cagttaatga aggaaaaaga
       61 accttgataa ttgacttgga tatgcaagga aatacttctg atgtgatgaa cttaacgcat
      121 atgaacttca ctaacgaaga aggaggcgga gaaggtgaac cgattgttta cgaaaatact
      181 gtgactgacg tgttgataaa caatcttcca cctaaagagg ctgtctataa agtaatcaat
      241 aacctctata tacttccagc agatatgtcc tttgaaatgt atgatgattg gatcaaagac
      301 caatataatg attcattatc ccaattcaaa tatatgaaag aaaagctaga accattattt
      361 gatgagtttg acgtcatcta tttagatgtc ccaccttcta tttctgtata tagtaaatcc
      421 gcgatgtaca ttgcggactg ggcaatcgtc gtcttacaga cccaagtaaa atcgatgaga
      481 aatgcgatgc aatatcttga gtatatggac ttttttgtga aagaatttga tacaaattta
      541 tatgttgctg gtgttattcc tttcatgttg gaaagcggtg acgcagtaga tcaggaaatg
      601 tataaacaag cccaagagat ttatggggaa catttgatta aaaatgttgt actcaaaaat
      661 gcgcgcttaa aacgatacga cggatcaggt attacgatgg agaagacaaa gaaaggtctc
      721 ttaaaacaat gggatcgcaa aagccacgaa ttatttatca gtattttgaa agaattggaa
      781 gaacacgaga attggtatgc ggaggtggag taattggctg acatcaaagg acttgatatt
      841 tttgggaaca acaaaggact cgccaaaata aaaaaagaag cagaagcaaa gaaaaagcag
      901 caagcaatta caccatctga aaataagcaa ttagaagtgc accaagttca agaaataaaa
      961 acttcctcca atgttgaaag acagagtaga ggaagaacaa aaaaagttgg cgcaccaatc
     1021 agaaagtttg ataaggttta ttccaccaag catcctttaa aactgtcgac tttgttaaat
     1081 gcgacttcca gagttttagt agaaaagtat gagactaata tgacaagaga tgaattgctt
     1141 agaaaagctt tagatgagta tattaaacag aatcttacgc aagaagataa gcaagatttg
     1201 tttaatgacg tggtgaaaga actagacttg ttcagagaaa aatatcc
//
LOCUS       DASHRQ010000174         1226 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.186, whole genome shotgun sequence.
ACCESSION   DASHRQ010000174 DASHRQ010000000
VERSION     DASHRQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.186"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            316..570
                     /locus_tag="U2M88_002897"
     CDS             316..570
                     /locus_tag="U2M88_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEM8375205.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="U2M88_002898"
     CDS             571..888
                     /locus_tag="U2M88_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEM8375206.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          428 a          202 c          245 g          351 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 gacaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaata
//
LOCUS       DASHRQ010000175         1198 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.187, whole genome shotgun sequence.
ACCESSION   DASHRQ010000175 DASHRQ010000000
VERSION     DASHRQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.187"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1154
                     /locus_tag="U2M88_002899"
     CDS             <1..1154
                     /locus_tag="U2M88_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEM8375207.1"
                     /translation="NGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNL
                     ESDSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAII
                     SLIYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTID
                     DLEFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINEC
                     IEIVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYI
                     LDENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDK
                     IFDVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
BASE COUNT          544 a           86 c          163 g          405 t
ORIGIN      
        1 tgaatggaaa agaaataaaa tatatagaac aaactttgga gttttattcc aagagtagtg
       61 aaataaaagt attcataaaa gaaaaaaata ataagaatga aatacaaatc aaagataata
      121 taaatgttaa tttagaaagt gatagtaatt ctttgataat tgaagaaaga gaaataaagc
      181 ttaatgatgg taaaaaaaca aatctacaat ttgtttctac agctgatatg caaaaagatg
      241 cgaaagattt aagccttaaa tttttaccat attctttatt aatatcaatt ttattttctg
      301 ctattatttc tttaatttat gcaaaatcaa taaaaaataa tatacaagaa ataaaaattg
      361 ttactgataa aatgatgaaa cttgataaaa aaatgagttt aaaagttagc tctaatgatg
      421 aagttggaga attaaaacaa caaattaatg atttgtattc tactttatta agaacaattg
      481 acgatttgga atttaaaaat aaagaaattt taaaattaga aaagttaaaa tatgactttt
      541 ttaaaggtgc atcccatgaa cttaaaaccc ctcttgctag tcttaaaata atactggaaa
      601 acatgaaata caatattgga aaatataaag aaagagatat ttatattaat gaatgtattg
      661 aaatcgttga tagtttaaca aaaaatattt ctcaaatatt atcagttcat tctatagaaa
      721 atttgaataa tgatgaagaa tatttgaaaa taaatgatac attagaagac atattaaaaa
      781 agtatgaaat attagcaaat caaaagaaaa taactgtcaa taattatata ttagacgaga
      841 atgtttatat aggaaaaaca gctttaaaga ttattctatc taatttgatt agtaatgcgg
      901 taaaatatac tgatgtaaat ggggtaatta atattgggat agttaatgat tggttatata
      961 tagagaattc atacggtaac aataaaattt cgaatatgga taaaatattt gatgttaaat
     1021 ttgatttaaa taaggaaaat agtaatggat taggtttata tattgtaagt aatattctga
     1081 ataattacaa tatagaatat aaagtattgc aggatgaaga attttttatc tttaaaataa
     1141 aaatattttc ttaattaaga tataattttg caatagttat tgaaaaatag agacttat
//
LOCUS       DASHRQ010000176         1193 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.188, whole genome shotgun sequence.
ACCESSION   DASHRQ010000176 DASHRQ010000000
VERSION     DASHRQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.188"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1076
                     /locus_tag="U2M88_002900"
     CDS             <1..1076
                     /locus_tag="U2M88_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEM8375208.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DASHRQ010000177         1140 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.189, whole genome shotgun sequence.
ACCESSION   DASHRQ010000177 DASHRQ010000000
VERSION     DASHRQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.189"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            4..963
                     /locus_tag="U2M88_002901"
     CDS             4..963
                     /locus_tag="U2M88_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEM8375209.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          385 a          210 c          227 g          318 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagatcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
//
LOCUS       DASHRQ010000178         1061 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.190, whole genome shotgun sequence.
ACCESSION   DASHRQ010000178 DASHRQ010000000
VERSION     DASHRQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.190"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(36..992)
                     /locus_tag="U2M88_002902"
     CDS             complement(36..992)
                     /locus_tag="U2M88_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375210.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DASHRQ010000179         1042 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.191, whole genome shotgun sequence.
ACCESSION   DASHRQ010000179 DASHRQ010000000
VERSION     DASHRQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.191"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(73..318)
                     /locus_tag="U2M88_002903"
     CDS             complement(73..318)
                     /locus_tag="U2M88_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375211.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            663..947
                     /locus_tag="U2M88_002904"
     CDS             663..947
                     /locus_tag="U2M88_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375212.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
BASE COUNT          350 a          182 c          155 g          355 t
ORIGIN      
        1 tagtttttgg tgtatttcat tggtccaagc taaaaaactc ttagaagcga ttttagagcc
       61 atttaaaccc acttaattat attctccatg aaattcgctc catgcgtcaa aagctgcgtc
      121 tgaaattccg ccgtcttcgt tcactagttg cattaatttt tccaacgatc gttttctttc
      181 gggtgtcata tttttatatt ccaacaattt ttcgtctgtt aaatcgaact tgattgcttg
      241 aattgcttgc tctaattttt ctaagttttg aaaatattcg taaggaaatt tttccatttt
      301 ttctttctcc agtctcacaa tttttctaga aataaaatca cttctttgcc gtctatactc
      361 tcaaaaaaaa tgtcgtttga aaaactaaat aaatttttga tcgtgcattt ttaaatttgg
      421 ttatcgatta cacgtgcagc actccccccc gaattttaaa gagcataagg aaacgtgtac
      481 ccactttcta aaaaaagtgg gtagataggc tgtgcctatc acgtttctca gaaaaaaatt
      541 tgatatcact ttttcgccat cgtgggtagc ttatgtatag cattttggca aaaaacagct
      601 atacaaatat agtatttgta tggtatacat aaattataca taattgcaaa aggagatttt
      661 ttatggacat tttagttcgg aatattgatc caaagtttgt tgcaacaata gacaaacgtt
      721 gccaagaact cacgaataaa acaggagaaa aatggagtcg aaataaatat ctaaaagttt
      781 taatcgaaag tgattttgac cgtcaactta cgacttacaa aaaagaccaa ttcgatttat
      841 tgctcgataa atttattgct atccaaacag aaaatactca aatattaagc gagtatgtct
      901 acacaaataa tcaaatcatt gaaatgctta tcggaggaat gatataacca tgagaaaaac
      961 ttctgtccaa aaacatgtta ctttgtaccc tagtcatgtt gacgttttag attccatcat
     1021 cgaaaaaaat ccagatatca at
//
LOCUS       DASHRQ010000180         1024 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.192, whole genome shotgun sequence.
ACCESSION   DASHRQ010000180 DASHRQ010000000
VERSION     DASHRQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.192"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..292
                     /locus_tag="U2M88_002905"
     CDS             <1..292
                     /locus_tag="U2M88_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM8375213.1"
                     /translation="QKTYPKVTQSLIKNQDLLTFYEFPPGIRRSIYSTNLIESFKKQI
                     KKYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE"
BASE COUNT          368 a          182 c          132 g          342 t
ORIGIN      
        1 gcaaaaaact tatcctaaag tgacgcagtc actgataaaa aatcaagatt tattgacttt
       61 ttatgagttt ccgcctggta tccgccgaag catttactca actaatttaa tcgaatcttt
      121 caagaaacaa atcaagaaat acagccgcag aaaagagcag tttcaaaatg aagaatcact
      181 agagcgtttc ctagtatcca tctttgatac atacaatcaa aaattcttaa atagaagcca
      241 taaaggcttc caacaagtga cggatacatt agcttcaatg tttactgagt aacccattat
      301 tttgcagaag gacgagttat ttacacaaaa ttattgacgc tccctccaaa ttacttcatc
      361 aaaacattga tataattttt attatatcag tatttattct tgatacataa atgagaaaat
      421 aaatacttta aatctattga ctacaagtag tcattaagtc ttcataagat gtacaatatg
      481 acattaaaaa aataaaagct tacgaccatc cgttaaatct gacactaaat aatggactgc
      541 tttcttttgt attagcaggt tacgttgact atgaatattc accagattat ttggagaaac
      601 ctcttcaaca cgagaacaat gatattaatt ttagatttgt ctacgagtca aaaagtaaaa
      661 catggttagt tgatcaatta agcacgataa acacaagctt ccatgaatta cctaaacctg
      721 aaaatgaagt tgagatggtc attaaaatct tctaaaaaag aagcgttgaa ggggaaatat
      781 tcctttcaac gctctttttt atatgcttag aaacataaat aaattgtata ctttcttttt
      841 agcccctatt acttttcacg aaaaatatct ttttttcgac gctagcgttt ctctacttct
      901 aatactgtac acagtttttg tattattgaa aaattactca ttatttagca ttaatccaca
      961 tcgttttatc aatcgacctt catactaaac tactttaaag tcatcttcct tctcgttttt
     1021 catt
//
LOCUS       DASHRQ010000181         1000 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.193, whole genome shotgun sequence.
ACCESSION   DASHRQ010000181 DASHRQ010000000
VERSION     DASHRQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.193"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            369..1000
                     /locus_tag="U2M88_002906"
                     /pseudo
     CDS             369..1000
                     /locus_tag="U2M88_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DASHRQ010000182          892 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.194, whole genome shotgun sequence.
ACCESSION   DASHRQ010000182 DASHRQ010000000
VERSION     DASHRQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.194"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..847
                     /locus_tag="U2M88_002907"
     CDS             <1..847
                     /locus_tag="U2M88_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEM8375214.1"
                     /translation="TKVKRNWLPRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILP
                     SRKKKALVSYFMQFERRARLKVKVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLI
                     RKFEDIRVRIMKSFDRNDPTQAKYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYL
                     TESEVVDRLLSISDELRIAYFYYQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKK
                     AFINYESGIRLALELPYSNAKIENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITY
                     ESKNI"
BASE COUNT          286 a          175 c          164 g          267 t
ORIGIN      
        1 gacaaaagta aaaagaaatt ggttaccgcg ttgtttatta atggatgaat ttcgttcact
       61 gaaaaatcaa gtcggtccct actccttttc ttgtatggat ggcgatactg gaaaattact
      121 ggatattctt ccttcacgga agaaaaaggc tctcgtttct tattttatgc aatttgaacg
      181 acgcgcacga ttgaaggtaa aggtcatcgt gacggacatg aatgtttctt atgcctctct
      241 gataaaggaa tgttttccaa aggcaaaact cgttgtggat cgttttcaca ttgtcaaaca
      301 tctcattcgt aaatttgagg atattcgcgt acgaatcatg aaaagctttg atcgaaacga
      361 tccaacacaa gccaaatatt atcgacaatt aaaggcgttg tctcggcttc tgatcaaacg
      421 acaggacaag cttgtttatg acaaatggac caaatggcgc aattttggtt gggcgtatct
      481 caccgaaagt gaagtggtgg atcgtctgct ttcgatctct gacgaattac gaatcgccta
      541 tttttactat caagaaattc ttcaagcgtt tcacgacaaa gaggcggaca ctttctttaa
      601 acttgtaaga acgatgccca attccgttcc aaaagaactg cattctataa aaaaagcttt
      661 tataaactat gaatccggca ttcgtttagc cctcgaattg ccttattcaa atgccaaaat
      721 agaaaatctg catacccaca tcaaagcatt gaaacgagtc gcttatggct ttcgaagctt
      781 tcggaaaatg aagaccagaa ttttcttact aaataatctg attacctatg aatcaaaaaa
      841 catctaaagt agaaaaggaa ttctacctta gatgttctta tctcactcta tc
//
LOCUS       DASHRQ010000183          863 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.195, whole genome shotgun sequence.
ACCESSION   DASHRQ010000183 DASHRQ010000000
VERSION     DASHRQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.195"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..861
                     /locus_tag="U2M88_002908"
     CDS             <1..861
                     /locus_tag="U2M88_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEM8375215.1"
                     /translation="EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPD
                     KYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKR
                     YKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIIS
                     YSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMS
                     RKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLS
                     PVEYRKQSIK"
BASE COUNT          374 a          112 c          140 g          237 t
ORIGIN      
        1 gagcaagaag aaatcaaggt taaaacaatc cttcagctca aaaaagaatt taaattgaat
       61 ctattacttt caattgcaca attagcgaag tcaacttatt attactgggt aaaaaaatta
      121 gataagccag ataaatatag caagataaag caagaaatta cagcgattgt aaaagaatca
      181 agaaactctt atggttatcg tagagtcact ttagcgttaa agatgaaagg atatacaatc
      241 aatcataaaa cagttagaaa attaatgtcc caaatgggac ttacctgtca aatccgaata
      301 aagcgatata aatcttataa aggaacagta ggaaaaattg ccaagaatgt gttaaaacga
      361 aatttttcag tagatacacc caataaaaaa tgggtgacag acgtcactga attcaagata
      421 aaaggaagaa aaatctatct atctcctata cttgatttat ttaacggaga aataattagt
      481 tatagtatat caactagtcc aacatataaa ctgattgaag aaatgcttca acaagcaatt
      541 aaaaaaaagg gaactgaggg ttcactgatt ctacattcag atcaaggatg gcagtatcag
      601 atgccacaat atcaaaaaaa attaaaagag aataacatta ttcaaagcat gtctagaaaa
      661 ggaaattgct tagataattc tgtaatagaa aatttttttg gtgtgcttaa atcagagttt
      721 ttttatcgag aaaaattccg atcaattgag atatttcaaa gtaaattaaa tgagtatatt
      781 aggtggtata ataacaaaag gataaaacta aagttaaatg gactatctcc agtagaatat
      841 cggaaacagt ccattaaata gtt
//
LOCUS       DASHRQ010000184          859 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.196, whole genome shotgun sequence.
ACCESSION   DASHRQ010000184 DASHRQ010000000
VERSION     DASHRQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.196"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            28..>859
                     /locus_tag="U2M88_002909"
     CDS             28..>859
                     /locus_tag="U2M88_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM8375216.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     V"
BASE COUNT          297 a          149 c          165 g          248 t
ORIGIN      
        1 ccaaagaatt ataaggagtg agtacaaatg acctataccc atcttacaac ggatgaactt
       61 gtaataatag agtcttattt caaaatgaat caatctgttg ctaaaactgc ccattgcttg
      121 aatcgttcaa gacaaacgat ccataaagta tatctgttct tcaagcaagg aaaatcagcc
      181 ttagaatatt atcaacagta taagaaaaac aaatcaaact gtggtagacg tccgcttgtt
      241 ttacccgagg aacaatcaga atatattcaa agaaaggttg ttcaaggatg gacacccgat
      301 gtgattgttg gtcgtgcagc gtttcctatt agttgttctg ctcgtaccat ttatcgtatg
      361 ttcaaaaagg ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat
      421 ggacatcaag aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag
      481 gattatagcc aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg
      541 aaacataaaa gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg
      601 aaaccgtgtg gtagacaagc gattgatatt gaaaaaaaat taaatcaatg gtttgaatct
      661 gtaccgaaaa acctattcaa atccatcact tttgattgtg gaaaggaatt ttcaaattgg
      721 aaacagatca gtaatgtcaa tgatattgcc atttatttcg ctgatccagg aacgccgtct
      781 caaagaggcc taaacgagaa ttctaacgga ttgttacgta gagatggttt attgaaatct
      841 atggatttca attcagtag
//
LOCUS       DASHRQ010000185          853 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.197, whole genome shotgun sequence.
ACCESSION   DASHRQ010000185 DASHRQ010000000
VERSION     DASHRQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.197"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..205)
                     /locus_tag="U2M88_002910"
     CDS             complement(<1..205)
                     /locus_tag="U2M88_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8375217.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVI"
     gene            complement(275..>853)
                     /locus_tag="U2M88_002911"
     CDS             complement(275..>853)
                     /locus_tag="U2M88_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEM8375218.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          243 a          175 c          139 g          296 t
ORIGIN      
        1 ctataacatc aagtttaaat tttgtagtgt agcttttgcc acctccaacc aagccttccc
       61 aaccatgata gttataaatc cttacccaat gtctaaccag tgtacgattt atttgatatt
      121 tatgtgcaag atatttgtag ccgccttcgt tatttaaata gtctgaaact acttttttct
      181 taaaaacaaa tgtatatttc cgcaaaaaaa gcaccccttt taattagatt tctagtctaa
      241 cttttggggt gcacatcaaa gaggggggaa ttttttatcg ttgtggaata tttaaatacc
      301 aacgcttgtc atgaaaatct tgtgctccac ccttagtatt tccttctgga tcgtttgtcg
      361 cacgcatcat gacgtatact ttcttattag ggaaatcacg catattgaaa gatacatgat
      421 agccaacatt tcctaaagta ttataagctt gatttacgtc tggtctataa atcccatcag
      481 ctcttactct agctagttct tttccagtat tgtagtccat aatgaaaata tactcgtatt
      541 tataattagc aatgtgccat ccagctacat gcaagtttgc gttttcgatt tctccgaact
      601 gatcaacgtg ggcgtaattt gttccatctg tcagcgtaga atttgcagca cctgctcgag
      661 ttggatcaat tactggttta tcctccgaag tagttggatt atcatccgaa gtagttggat
      721 tatcatccgt aaaaccatga gccaaatcat aggctagttt ttctttactt acacccattt
      781 cagaaagata accgcaagga tctgtatgat cgccccaaat attttgtgtt acccataaat
      841 gcgatttgat tcc
//
LOCUS       DASHRQ010000186          789 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.198, whole genome shotgun sequence.
ACCESSION   DASHRQ010000186 DASHRQ010000000
VERSION     DASHRQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.198"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..782
                     /locus_tag="U2M88_002912"
     CDS             <1..782
                     /locus_tag="U2M88_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM8375219.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          106 c          102 g          241 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgccc
//
LOCUS       DASHRQ010000187          783 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.199, whole genome shotgun sequence.
ACCESSION   DASHRQ010000187 DASHRQ010000000
VERSION     DASHRQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.199"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..672
                     /locus_tag="U2M88_002913"
     CDS             <1..672
                     /locus_tag="U2M88_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM8375220.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          290 a          120 c          146 g          227 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 cca
//
LOCUS       DASHRQ010000188          777 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.200, whole genome shotgun sequence.
ACCESSION   DASHRQ010000188 DASHRQ010000000
VERSION     DASHRQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.200"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            307..603
                     /locus_tag="U2M88_002914"
     CDS             307..603
                     /locus_tag="U2M88_002914"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEM8375221.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLSTVCMKKLDVC"
     gene            complement(526..777)
                     /locus_tag="U2M88_002915"
                     /pseudo
     CDS             complement(526..777)
                     /locus_tag="U2M88_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          260 a          152 c          117 g          248 t
ORIGIN      
        1 cactctctat tattcttttc ataaatattg taaatagaag tattaaaaat atatacaaaa
       61 agtattattt cgagaaagat aaatattttc aaaaaccttt tcataatcct caccctttct
      121 tacactgata aatcatgcgt ctaatattca aattatactc gatatgcttt agctaatcag
      181 ccaactctgt ttgtctacta tcattatatc aatagacaat atctgctccc gtctttatga
      241 tcgtgccaaa gcaatcaaaa gaaaattgcg aatcacttag atcgaaagtt tactcaaaca
      301 aaagcattgt cagcggttgt cacggattta acttatgttc gggtcgggac aaattgggct
      361 tatatttgtt tgatgattga tttattcaat cgtgaaatta ttggccattc ttgcggaaat
      421 aaaaaagatg cgcagcttgt aaaaagggcc attcaaagca ttccttactc acttcaagaa
      481 atcgaactct tccatacgga tcgtggaaaa gaatttgata accaaaccat tcagaatctc
      541 atgaatggtt tggttatcaa attcctttcc acggtctgta tgaaaaagct tgacgtctgt
      601 taaagaatac ggtatccgtc caaaggcttc ttttaccaat gaggcatctt tcttctcacc
      661 acaggaataa ccaataattt ctcgattaaa caaatcaagt attaagcaga tataatgcca
      721 ctttttcccc acgcgaacat aagtaagatc cgtaacgatg gcttccaatg gctgttc
//
LOCUS       DASHRQ010000189          775 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.201, whole genome shotgun sequence.
ACCESSION   DASHRQ010000189 DASHRQ010000000
VERSION     DASHRQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.201"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(249..728)
                     /locus_tag="U2M88_002916"
     CDS             complement(249..728)
                     /locus_tag="U2M88_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375222.1"
                     /translation="MCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNSL
                     DFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKCFESAVVKFPQFKLNP
                     AASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKKAFKIKTIGTAPNFK"
BASE COUNT          262 a          106 c           87 g          320 t
ORIGIN      
        1 tgacgctccc ttaacaataa atgaagactt ttttattgtt aattgtttat ttttctatta
       61 ttagatatga aaatcgcatt tagataaaat agaaaaatat gaatatattt taaatattaa
      121 gatgagagtc attaacttat gatagtagtc aatttgcttt tcactaatta caaaatatgt
      181 gatataggaa gtttagttat tgttctcata atgccatcct gtttttagat tatctagtaa
      241 tttttttgtt atttaaaatt aggagcagtg ccaattgttt taattttaaa ggcttttttt
      301 atatcttcta aattagaaga tatttgtata tcaatatgga atatattacc ttttttgtat
      361 ttttttatgt tatctttagc taaaacatca aatacatgtc cttgaaattg tatcgaggca
      421 gctggattta gtttaaattg aggaaacttt accacagctg attcaaaaca cttgtcacat
      481 ttttttattt ttttaccttt gtaaaaatat tcgattgaat attctatttg gcacgactca
      541 cttggcagta tatgtatgtc aatatttttc atctgtttca taggtatttc aaaatctaaa
      601 ctattaaaaa catataattc cctgtaatta tttacatcta actctactaa atttatcttt
      661 ggaacaatag atcctccacc cattccacca taataaaaaa aatcttgttc tctatcaaaa
      721 tagcacatat taagctcagt ttgtttatca tcctcaataa ttttataaat caaac
//
LOCUS       DASHRQ010000190          742 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.202, whole genome shotgun sequence.
ACCESSION   DASHRQ010000190 DASHRQ010000000
VERSION     DASHRQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.202"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            176..379
                     /locus_tag="U2M88_002917"
     CDS             176..379
                     /locus_tag="U2M88_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HEM8375223.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            379..729
                     /locus_tag="U2M88_002918"
     CDS             379..729
                     /locus_tag="U2M88_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEM8375224.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          299 a           80 c          119 g          244 t
ORIGIN      
        1 cttccttttt tgtatggatt ttttcattta cacaaaatat tttacactct cgtcaatcat
       61 tgttatataa taaaatgtta tataataaaa ttactaatga tatttaatat tacaagcaat
      121 cttaagaaat tcgaaaagga aaaaatgatt atgaaaaaga aagaaaaaga aagtattgaa
      181 acattgtgtt attctaggaa tattaggaac ttgtctagct ggcattggta caggaataga
      241 cgttgacgca gctacttact atggaaatgg tctttattgt aacaaagaaa aatgttgggt
      301 aaattgggga caatcatggt cagaagggtt aaaacgttgg ggagataatc tttttggtag
      361 ttttagtgga ggacgataat gacttcaata aacattgatc acacaaaaaa agtcatacat
      421 gaattataca atagtttaat gacacgacct aatcagacga aagcattatt agatattact
      481 gatgttttat ttcaggtata taaaaaaatt gatacagcaa aatatcctga aattcttgta
      541 aataaattag ttaattatat ttacatcgtg ggttttgata ataaaatacg ctttattgat
      601 aatgatgaaa aattattaat agaattaggg gatatttcta aaaaagctgg aattaatagt
      661 aaatacaaag caaactttac agataaatcg caattttata gttattcaga aacaatgcct
      721 caacgataaa atatagtata ct
//
LOCUS       DASHRQ010000191          666 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.203, whole genome shotgun sequence.
ACCESSION   DASHRQ010000191 DASHRQ010000000
VERSION     DASHRQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.203"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            55..666
                     /locus_tag="U2M88_002919"
                     /pseudo
     CDS             55..666
                     /locus_tag="U2M88_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          274 a          112 c          125 g          155 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atcaatcaaa accaaagaaa gacgaatcag aacttgaaga agtagtcgct gaagaattta
      481 tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa agccttatca aaacgaggca
      541 ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa tagaggatta aaatcgagct
      601 atactgttgc ttattttaaa gtacatcatt ctacttgcaa tgaagccaaa acgacaaacg
      661 tattga
//
LOCUS       DASHRQ010000192          658 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.204, whole genome shotgun sequence.
ACCESSION   DASHRQ010000192 DASHRQ010000000
VERSION     DASHRQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.204"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>658
                     /locus_tag="U2M88_002920"
     CDS             <1..>658
                     /locus_tag="U2M88_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM8375225.1"
                     /translation="ISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAEN
                     IADHIESFTDYDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMEL
                     LSVSMLIVISTFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAG
                     VYFVTQNSDDLLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLE
                     N"
BASE COUNT          194 a          118 c          147 g          199 t
ORIGIN      
        1 atttcaagtc gagacgggga gaagtttcct gtgttacgta aagccattcg aacggtgact
       61 caaagtgatc aacgggggtt attacaagtt attaacgaat tgcgaaaaga cgggtcacca
      121 atcgcggaaa atatcgccga tcatatcgag tcctttaccg attatgattt tgctcatttg
      181 cttttctcag acggctcgat tacccaatcg attagtttag ataaacagct aaatatcatt
      241 caagtggcgg atttggtttt accggatgca gaaacgactt ttgaagaata taccaccatg
      301 gaattgctta gtgtttcgat gctaatcgtc atttccactt ttgccttgga ctttatccat
      361 agtaaccgag aaatcttcaa aattgtcgat ttggatgagg cttggagttt tcttcaagtg
      421 gctcaaggaa aaacgttatc gaataagctt gttcgtgctg gtcgggccat gaatgctggg
      481 gtctatttcg tcactcaaaa tagcgacgat ttattagatg aacgtttaaa aaataatatc
      541 gggttgaagt ttgcttttcg cagtaccgat attcatgaaa tcaagaaaac cttggaattc
      601 tttggtttgg atcaggagga tcaaagcaat caaaaacgcc tgcgagattt ggaaaacg
//
LOCUS       DASHRQ010000193          656 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.205, whole genome shotgun sequence.
ACCESSION   DASHRQ010000193 DASHRQ010000000
VERSION     DASHRQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.205"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="U2M88_002921"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="U2M88_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEM8375226.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..>656)
                     /locus_tag="U2M88_002922"
     CDS             complement(481..>656)
                     /locus_tag="U2M88_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375227.1"
                     /translation="VPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNYI
                     LQAILKELLSNAL"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DASHRQ010000194          618 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.206, whole genome shotgun sequence.
ACCESSION   DASHRQ010000194 DASHRQ010000000
VERSION     DASHRQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.206"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>618
                     /locus_tag="U2M88_002923"
     rRNA            <1..>618
                     /locus_tag="U2M88_002923"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          185 a          120 c          187 g          126 t
ORIGIN      
        1 agtgaccgat agtgaaccag taccgtgagg gaaaggtgaa aagcaccccg gaaggggagt
       61 gaaatagaac ctgaaaccgt gtgcctacaa caagtcaaag cccgttaatg ggtgatggcg
      121 tgccttttgt agaatgaacc ggcgagttac gattgcatgc gaggttaagt tgaagagacg
      181 gagccgcagc gaaagcgagt ctgaataggg cgtttgagta tgtagtcgta gacccgaaac
      241 catgtgatct acccatgtcc aggttgaagg tgcggtaaaa cgcactggag gaccgaaccc
      301 acgtacgttg aaaagtgcgg ggatgaggtg tgggtagcgg agaaattcca aacgaacttg
      361 gagatagctg gttctctccg aaatagcttt agggctagcc tcggaattga gaatgatgga
      421 ggtagagcac tgtttggact aggggcccat ctcgggttac cgaattcaga taaactccga
      481 atgccattca ttcatatccg ggagtcagac tgtgagtgat aagatccata gtcgaaaggg
      541 aaacagccca gaccaccagc taaggtccca aaatatatgt taagtggaaa aggatgtggg
      601 gttgcacaga caactagg
//
LOCUS       DASHRQ010000195          548 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.207, whole genome shotgun sequence.
ACCESSION   DASHRQ010000195 DASHRQ010000000
VERSION     DASHRQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.207"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>548)
                     /locus_tag="U2M88_002924"
     CDS             complement(<1..>548)
                     /locus_tag="U2M88_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEM8375228.1"
                     /translation="SLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW
                     ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPT
                     IFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG
                     NDDLLEKYMSGKSLEALELEQE"
BASE COUNT          147 a          119 c           84 g          198 t
ORIGIN      
        1 tcctcttgtt cgagttccaa tgcttctaat gatttaccgg acatatattt ctctaaaagg
       61 tcatcgtttc cctctattac cgtatcccat tgttcagatt cggtaaagtt cgtcacacac
      121 atattaggat acagttctac cttctgtttg attacaattt cggcagaaag tttctcttta
      181 atatcctgat aaaccgttga taaatcaatt ccattttggt caatcttatt gataaaaaag
      241 attgtgggaa tccccatttt cctaagtgca tgaaataata tacgagtttg tgcttgtacg
      301 ccatcttttg cagaaatcag tagaattgcc ccatctaaaa ctgataatga acgatatact
      361 tctgctaaga aatccatatg tcctggcgtg tctatgatgt tcaccttcgt attttcccac
      421 tgaaaagagg ttattcctgt ctgaattgta attcctctct gacgttctaa aagcgtatta
      481 tccgtcctcg ttgtaccttt gtccacgctt cctaattctg taatcgctcc actgttatat
      541 aataagct
//
LOCUS       DASHRQ010000196          546 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.208, whole genome shotgun sequence.
ACCESSION   DASHRQ010000196 DASHRQ010000000
VERSION     DASHRQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.208"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(271..534)
                     /locus_tag="U2M88_002925"
     CDS             complement(271..534)
                     /locus_tag="U2M88_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HEM8375229.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          213 a           97 c           75 g          161 t
ORIGIN      
        1 actctcctga attttttcat gttgaaaaca tcactaatca aaggcaaatt ataatttaga
       61 aatagaatga aaacactaaa tgaaaataga acattagaaa aaatagaatc aaaaaaaccc
      121 aagactagtg ccgaaataag ggaaacaatc gaagctacaa aaaataatga tttcgttgaa
      181 caggaccaat ttatttgttt ccgatatttc tcaaaaagaa ctttctttgg gaaagttaaa
      241 ggcgactgta aagtcgcctt tagtcctttt ttaccaagct gctgcatatt tagctccata
      301 ttttttaatt aatgatttgg ctgtagcaac aataccagct gtaattaatc cgcctccaac
      361 aactgcagca acaatagaaa tgattgtagc aaccgtgcta ccagtattaa ttatgtcaat
      421 aactttttta gctgcataag aagaaattcc aatgttagca cttagcattg gtacaccaac
      481 aaagaatccc gcaaaaccta aaattggtaa aactaataat aaattctttt tcataataat
      541 tcctct
//
LOCUS       DASHRQ010000197          528 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.209, whole genome shotgun sequence.
ACCESSION   DASHRQ010000197 DASHRQ010000000
VERSION     DASHRQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.209"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(5..90)
                     /locus_tag="U2M88_002926"
     tRNA            complement(5..90)
                     /locus_tag="U2M88_002926"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="U2M88_002927"
     tRNA            complement(107..178)
                     /locus_tag="U2M88_002927"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="U2M88_002928"
     tRNA            complement(190..262)
                     /locus_tag="U2M88_002928"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="U2M88_002929"
     tRNA            complement(280..361)
                     /locus_tag="U2M88_002929"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="U2M88_002930"
     tRNA            complement(369..441)
                     /locus_tag="U2M88_002930"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="U2M88_002931"
     tRNA            complement(445..517)
                     /locus_tag="U2M88_002931"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DASHRQ010000198          525 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.210, whole genome shotgun sequence.
ACCESSION   DASHRQ010000198 DASHRQ010000000
VERSION     DASHRQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.210"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..134
                     /locus_tag="U2M88_002932"
     CDS             <1..134
                     /locus_tag="U2M88_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM8375230.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT          220 a           75 c           56 g          174 t
ORIGIN      
        1 aagtgaatca gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat
       61 cattgaatta cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt
      121 ctagcttaat ttgacaaatc atacaaacaa aaataaataa agtcggattt ttctgataat
      181 tcaacgcaaa aaaacgaaca atttttcaat aaatcagtct tctaaaatag ataacctaaa
      241 aaaaacgaac aaacaaataa aatgactgtc cttcattcac taaacatatt tttatttttt
      301 atattgttaa aaattcacaa ttatttttta atagaattat ctgttttcca aattttataa
      361 cataaaaaac cttttttaac ggcatttata tgctttttca gaaattaact gatgatttca
      421 aaaagaaaac atgtcataat atttcccata ttgtgcaaaa aaatacaagg tggaggctct
      481 actatgattg aagaatatat tgagaaaaat attttaagac agctg
//
LOCUS       DASHRQ010000199          516 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.211, whole genome shotgun sequence.
ACCESSION   DASHRQ010000199 DASHRQ010000000
VERSION     DASHRQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.211"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            105..>516
                     /locus_tag="U2M88_002933"
     CDS             105..>516
                     /locus_tag="U2M88_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM8375231.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGV"
BASE COUNT          185 a          102 c           92 g          137 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgttt
//
LOCUS       DASHRQ010000200          509 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.212, whole genome shotgun sequence.
ACCESSION   DASHRQ010000200 DASHRQ010000000
VERSION     DASHRQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.212"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..132)
                     /locus_tag="U2M88_002934"
     CDS             complement(<1..132)
                     /locus_tag="U2M88_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEM8375232.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DASHRQ010000201          489 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.213, whole genome shotgun sequence.
ACCESSION   DASHRQ010000201 DASHRQ010000000
VERSION     DASHRQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.213"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..415)
                     /locus_tag="U2M88_002935"
     CDS             complement(<1..415)
                     /locus_tag="U2M88_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375233.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFF"
BASE COUNT          186 a           78 c           62 g          163 t
ORIGIN      
        1 aaaaaaacgg ctttccaaat gatgaaatac ctaaatctaa cttattaaga aagttgaaat
       61 ttttatattc aggaaaccat ttattaattt catgtgcagc acgtaatgca ttccctggag
      121 ttgtcaaaga gaaaattaat tctgctaagc ttattattga aaatactgat aacttataat
      181 tatatcgtcc attgacaatc aaatataaac taactatact tgttagaaca aaaaaacagg
      241 cgttgacttg ttcttgatta gttaaaaata tcaataaagg aatacttacc caatatattt
      301 ttctatttat ttcttctcct cttaagagtt gaaaaaacgg ataaaaagtt agtaaactaa
      361 atgctactgg ccactgatag ttcaaggtgg ttgctctcca accggtttcc cccattactg
      421 tcaatggtat taaacagtat atagagcaaa ccaataataa atttttaaca tttattttct
      481 caaaaacat
//
LOCUS       DASHRQ010000202          487 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.214, whole genome shotgun sequence.
ACCESSION   DASHRQ010000202 DASHRQ010000000
VERSION     DASHRQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.214"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..>487)
                     /locus_tag="U2M88_002936"
     CDS             complement(<1..>487)
                     /locus_tag="U2M88_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEM8375234.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DASHRQ010000203          460 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.215, whole genome shotgun sequence.
ACCESSION   DASHRQ010000203 DASHRQ010000000
VERSION     DASHRQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.215"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(20..91)
                     /locus_tag="U2M88_002937"
     tRNA            complement(20..91)
                     /locus_tag="U2M88_002937"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="U2M88_002938"
     tRNA            complement(113..200)
                     /locus_tag="U2M88_002938"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="U2M88_002939"
     tRNA            complement(225..298)
                     /locus_tag="U2M88_002939"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="U2M88_002940"
     tRNA            complement(312..382)
                     /locus_tag="U2M88_002940"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="U2M88_002941"
     tRNA            complement(384..459)
                     /locus_tag="U2M88_002941"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          133 a          128 c          107 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac caactgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DASHRQ010000204          417 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.216, whole genome shotgun sequence.
ACCESSION   DASHRQ010000204 DASHRQ010000000
VERSION     DASHRQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.216"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
BASE COUNT          116 a          101 c           70 g          130 t
ORIGIN      
        1 attttgttcc tcctcaaaga aaaagagact tttggaaaga atttccagag ttcacgtaac
       61 cctaaaaatt catatccaaa aatctctagc ttgaagaaac ctaaaaatat tcgtataatg
      121 aacctgagtt acggagctag gtgaagtagt ttccgtaaat agtcgagagt gttagcgcac
      181 tgctcggctt ttattttgct cttttttctc gttttctttg attatagcac tcctcctaaa
      241 aaaataaaaa gacctcaggg aatttcattc cccgaacccc ttgagcctcc tcctgccgaa
      301 atggaaaggg cgcacccgct gtttatttca ttcagctatc ggctttcaca atctaacacg
      361 atcgttccgc taccagtcgc cttacaggct ccattttcgc tcctctaacg tgtttta
//
LOCUS       DASHRQ010000205          415 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.denovo.217, whole genome shotgun sequence.
ACCESSION   DASHRQ010000205 DASHRQ010000000
VERSION     DASHRQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.denovo.217"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..339)
                     /locus_tag="U2M88_002942"
     CDS             complement(<1..339)
                     /locus_tag="U2M88_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8375235.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DASHRQ010000206        21056 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.guided.047, whole genome shotgun sequence.
ACCESSION   DASHRQ010000206 DASHRQ010000000
VERSION     DASHRQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.guided.047"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="U2M88_002943"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="U2M88_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEM8375236.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="U2M88_002944"
     CDS             2039..2227
                     /locus_tag="U2M88_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEM8375237.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..2638)
                     /locus_tag="U2M88_002945"
     CDS             complement(2285..2638)
                     /locus_tag="U2M88_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8375238.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            3169..3591
                     /locus_tag="U2M88_002946"
     CDS             3169..3591
                     /locus_tag="U2M88_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM8375239.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            3588..3818
                     /locus_tag="U2M88_002947"
     CDS             3588..3818
                     /locus_tag="U2M88_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8375240.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            4316..4516
                     /locus_tag="U2M88_002948"
     CDS             4316..4516
                     /locus_tag="U2M88_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEM8375241.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            4543..5736
                     /locus_tag="U2M88_002949"
     CDS             4543..5736
                     /locus_tag="U2M88_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM8375242.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5804..7369)
                     /gene="guaA"
                     /locus_tag="U2M88_002950"
     CDS             complement(5804..7369)
                     /gene="guaA"
                     /locus_tag="U2M88_002950"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEM8375243.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(7428..8351)
                     /locus_tag="U2M88_002951"
     CDS             complement(7428..8351)
                     /locus_tag="U2M88_002951"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEM8375244.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            8547..9152
                     /locus_tag="U2M88_002952"
     CDS             8547..9152
                     /locus_tag="U2M88_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM8375245.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            9460..10476
                     /locus_tag="U2M88_002953"
     CDS             9460..10476
                     /locus_tag="U2M88_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEM8375246.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            10617..11918
                     /locus_tag="U2M88_002954"
     CDS             10617..11918
                     /locus_tag="U2M88_002954"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEM8375247.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11964..12626
                     /gene="deoC"
                     /locus_tag="U2M88_002955"
     CDS             11964..12626
                     /gene="deoC"
                     /locus_tag="U2M88_002955"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEM8375248.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            12647..13045
                     /locus_tag="U2M88_002956"
     CDS             12647..13045
                     /locus_tag="U2M88_002956"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEM8375249.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            13111..14193
                     /locus_tag="U2M88_002957"
     CDS             13111..14193
                     /locus_tag="U2M88_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM8375250.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            14405..15967
                     /locus_tag="U2M88_002958"
     CDS             14405..15967
                     /locus_tag="U2M88_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8375251.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15960..17093
                     /locus_tag="U2M88_002959"
     CDS             15960..17093
                     /locus_tag="U2M88_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8375252.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            17093..18049
                     /locus_tag="U2M88_002960"
     CDS             17093..18049
                     /locus_tag="U2M88_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8375253.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            18148..19314
                     /gene="deoB"
                     /locus_tag="U2M88_002961"
     CDS             18148..19314
                     /gene="deoB"
                     /locus_tag="U2M88_002961"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEM8375254.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            19338..20156
                     /locus_tag="U2M88_002962"
     CDS             19338..20156
                     /locus_tag="U2M88_002962"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEM8375255.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            20173..20880
                     /gene="deoD"
                     /locus_tag="U2M88_002963"
     CDS             20173..20880
                     /gene="deoD"
                     /locus_tag="U2M88_002963"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEM8375256.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         6784 a         3752 c         4510 g         6010 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgttctctg
      661 ttccctcttt atcatggaag tgcaaaaagt aatataggga ttgataacct tatagaagtt
      721 attactaata aattttattc atcaacacat cgaggtccgt ctgaactttg cggaaatgtt
      781 ttcaaaattg aatatacaaa aaaaagacaa cgtcttgcat atatacgcct ttatagtgga
      841 gtactacatt tacgagattc ggttagagta tcagaaaaag aaaaaataaa agttacagaa
      901 atgtatactt caataaatgg tgaattatgt aagattgata gagcttattc tggagaaatt
      961 gttattttgc aaaatgagtt tttgaagtta aatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt cgagtgcttt
     2401 taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt aatgatacaa
     2461 gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt gttaaataac
     2521 gtgggtcaat ttcaatcatt gttcccactt gttcacgagt gagaccccgt ttcattcgag
     2581 cctctttaat cgctaaacca acggctctaa aatcgtattt atcttctttt ttacgcatac
     2641 taaatcacct ctatacattt tactgttcct atataaccat aaacaggtat agaaaaacat
     2701 gttatatagt ttatgagttc ctatagaaca ctgtggaatt tattaatgcg atgaatccaa
     2761 tttcaaatag aaaggtcgct gattttattt acctatacaa ctttataaaa tttagtcata
     2821 ctcctagaaa gtagataaaa ttggcgactt aatggaggga aaagtcgcca tgagaaagat
     2881 aatctgtcat ctagttctct taattaagtt cttatcaaaa aaacttatcc acctgtactg
     2941 gatagatatg accatcaatg tggttttgca gattacatag atagaaaaat caaagcatat
     3001 gaacagagaa aacaatctgt ttatgtgctt ttttggtcac ttaataaata tcttttagga
     3061 tttctcttca tttatgcaac aaatccccct ttcgtgtcgg ggtttagtat gaagggagat
     3121 aaagaagcac atgccactcc ctttcacgac agaaaggggg tgaaaagcat gaaaccatct
     3181 tcttttcaag aaaccataga aaatcagttt gactatatct gcaaacaagt tattgaagat
     3241 gaacgaaaag attacttcaa acatctagac agactatcag caaaagaagt ctcgttttct
     3301 gatatagata actaccttgt caactctttt tctactgttg atacgtacgt cactgatttc
     3361 caactattta aattatttgg attaagaatc aatgttgaaa gtgatttatt aagtgaagcc
     3421 ctgcgtcatc tttcagagaa aaaacggaac attattttac ttcattactt tatggatatg
     3481 agtgatgtgg agattgcgga gttactaaat ttgaatcgtt cgactgttta tcgtcatcga
     3541 ataagtggac tagccatgat taaagaattc atgaaggagt gtaaagaatg aaaacagctt
     3601 atccaagggt tcccttcccc ctcattgtaa aggcgacaga tggcgacgtg gaagcaatta
     3661 atcagattgt taaacattat cgagggtata cctcaaaacg ttcattacgt cgcatgacag
     3721 acgaatatgg aaattctcat atggttattg atgaaaccct acgtggcaga atggaaacga
     3781 aactcatctc taagatttta tcgttcgaaa ttaaatagtc ccattttttc gtggaagcgt
     3841 gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt attccatcaa agcatattgt
     3901 gtgttcagta ggttttgata ggctaacaag ccaaacgttc tttgacaacc aaataaaggc
     3961 aatcgaatac gtttcggtaa gaaaagagct gatgaactaa cacgccatga cctacaaaat
     4021 tagtgagcga ttcatgttag taattcgaaa gtaatcttta gcaggattgc tgccatgact
     4081 ttcttatcgt gataatgata ctcccgtata gccatagtcc gagcgttaga agcgtcacag
     4141 gcaatgggta cggctacatg agaaccatgc agaagtgaaa ttcctgtgag ctttgctaga
     4201 gctgttcgat tgctaagata ttcttctctt gtcagtgtat tattataaaa ctatttgata
     4261 taattatagg tgaatattgg agttttccta gcctataatt gggaaaggag taataatgat
     4321 aacggtcaca aaaaaggatt tagttgagct aggatatgga acttcttttt cagcagacat
     4381 cataaaaaaa gcaaaagaat tgatgattac aaaagggcat atctactatc aatctcgaaa
     4441 gctagataga gtccctcgtg aagctgttga agaaattcta ggaattcggt tttcaaatga
     4501 aaacaggtct gagtagtttt tgcacagtaa ggagtaaaaa ctatggttaa agacccaata
     4561 aaaaaagcaa agaatggaac ctattatttt cgtgcaaatc taggttatga ttctaaaggg
     4621 aaaaaaattc aaaaatatcg tagtggattc actactaaaa aagaagcaag agaagaatat
     4681 tcgaaactgc tattgatgaa accagaagaa ttaagtgaga ataaggataa aatgacgttt
     4741 aaacattata tctttgaaat tttccttcca tggtataaga cacaggtaaa gctcagaaca
     4801 tatgaaaatc gcttaccaac tattaagaag catttctctt attttgataa aatggctgtt
     4861 tccgatattg agcctattga tgtgcaaaat tggcaactaa aattatcaaa gaagtgtaag
     4921 tcttcttatg taagagctgt tcaaggacta ttttcagttg ctatggatcg agcaattgtc
     4981 cttgggataa ccaccacaaa tccatctaag attattggca atgtgaaaaa gcaaaagtct
     5041 aaaattgaat tttggacaaa agaagaattt gaaaaagtaa tctctctaat ttacaaagaa
     5101 gattactatc aacacttttt atttatctct ctgtggtttt tatttatgac tggaatgcga
     5161 attggtgaag ctactgccat tcaatgggaa gatattgatt ttgactcagg agtactaacc
     5221 atcaacaaaa cactctatta caagaatcaa tcgaattata gatttgttga accaaaaaca
     5281 aaggcgagcg ttcgtcatat tgtattagat aaatgcacat taacttattt atccgaatgg
     5341 aaggaagtac aacaaaatct aattcagaca gactttgtaa tgagctataa cggtattcct
     5401 acccaaaaac atacactatc atatgctatt gaacgctatg ctaagctggc tggtatccat
     5461 cgaatcagaa tccatgggtt acggcattct catgcttcct tgttaatcag tatgggagaa
     5521 aatccactta tcattaaaga tcgtctagga cacgaagata ttgagacgac tttaggaacg
     5581 tatggacatc tttatcctaa cagtaatttc gaggtagcta acaaattaaa tggagccatt
     5641 tcctttaaag aagcaaaaca aaattatgat acttcaccaa agaatcaatt tactgtagac
     5701 tacttgcgaa acaaagaaat gaaaaaagtg caataaaagt gcagtaaaaa gataagaaga
     5761 gccagaaacc cttggtataa gaggatttct agctctccta caactattcc cactccactg
     5821 ttgctggtgg tttagaagtg atgtcataca cgatacggtt gacgtgggct acttcgttta
     5881 cgatacgtac agagattttt tgcaagactt cccatgggat acgggcaaag tcagcggtca
     5941 ttccgtcgat tgaagttaca gcacggatac cgactgtgta gtcgtaagta cgtccgtcac
     6001 ccataacacc gacagagcgg atccctggaa gaacagtgaa atattgccag atatcacggt
     6061 caagtccagc agctgcgatt tcttcacgca agatagcatc agattcgcgt acgatttcta
     6121 gcttgtcttc agtgatttcg ccaagaacac gaataccaag accaggacca gggaatggct
     6181 gacgccaaac gattgaatca ggcatgccta actgagtccc taattcacgg acttcgtctt
     6241 tgaataatgt gtttaatggt tcgatcaatt caaattgcat atcttctggc agtccaccta
     6301 cattatggtg agatttgatc gtctgtgctg tttctgtacc agattcgata acgtcagtat
     6361 acaacgttcc ttgtgctagg aaagagacgc cttcttcacc agcaagtttt gttgcttcgt
     6421 catcaaaaac atatacgaat tcattcccaa tgattttacg tttttgttct gggtcagata
     6481 cacctttcaa cttgtctaag aagcgatctc ttgcatcgac tttgatgatg ttcaagccga
     6541 attttccgcc tagactttcc attacttgtt ccgcttcgcc tttgcgtagc agaccgtggt
     6601 caacaaagat agatgtcaat tggtcaccga tcgctttttg taaaagaacc ccaacgacac
     6661 tagaatcaac gccacctgaa agaccaagca agactttttt gtcaccaact tgttcacgga
     6721 ttttttctac ttgcatatcg ataaagttat ccattgtcca gttaccttca cagtgacaaa
     6781 catcaaaggc aaagtggcgc agcaattcga ttccgtattc ggaatgacgt acttctgcat
     6841 ggaattggat accataaaaa ttacgtgttg tgtcttggat agaagcaatc ggacaatctt
     6901 tgcttgttcc gactgtttca aagccttcag gaactttagt taccaagtca ccatggctca
     6961 tccatactgt ttgttgtttt ggtgtttctt taaacaagac agcttgatcg cttgttactt
     7021 ctaacaaggc tttcccatat tcgcggtttt tagcaggctc tacggaaccg ccaagtttgt
     7081 acgtgatcaa ttgcatacca tagcaaatcc ctaatacagg aatacctaaa ttgaagatct
     7141 caggatcgat cccaaatgca ttctcatcat atacactgtt tggtccgcca gaaagaatga
     7201 ttccctttgg attcatcgct tttacttctt ctgcagtaat gcggtggctc aataattcgg
     7261 aaaagactcc aagatcacga atacgtcttg tgatcaattg gttatactga ctaccgtagt
     7321 ctaatacgat aatcttttca acgttcgtca tatcggcaac gtttgtcaaa tttattaccc
     7381 ctatcccttt aaattttatc tcaaaatagt caaactattt taaaatgcta atattttcgc
     7441 atgaaaatcg aatcgatgat atgattttca gttttatgga gaattatatc ggctcttcct
     7501 cgtgtaggca agatatattc ctgtaaattt ttcaaattca catttttcca tacatctttt
     7561 gccattttaa aggcttcttc acgatctccg atagcaaaac gataataata attctctgga
     7621 ttttggaaag ccgtatccag taacgcacca aaacgatcca aataccattt ttcgatcagc
     7681 acgggatcgg catcgacata gacagaaaaa tcaaaaaagt cgctgacgta gatttgctga
     7741 ttcgcaggta attgaagcgt attgatcccc tcaacgatca agatatcagg ctgctggatc
     7801 aattcgtact tgtctggaat gatatcatac acatcatgtg agtaaacagg tacttttatc
     7861 tcttcctgac cagattttac acggttcaag aaatcgatca gcatctccat atcataactt
     7921 tcaggaaatc ctttacggtc catgattcca cgctctttta atatacgatt tggataaaga
     7981 aatccgtcag tcgtgatcag ctgaacgttt cttcgtttga aggtacgaga caaaatcgtc
     8041 tgcagcagtc tggctgttgt actttttcca acggctacac ttccggcgat cccgataata
     8101 aaaggcggta cttctacgta tttatgaaga aataaacctt tactcaagaa caaagattca
     8161 aattctttca tataaagata aatcagatga gcaagaggta catagatatc tcgaacatcc
     8221 tgcatggaaa tgcggtcatt caaacttttg atattatcta gttcatcttg tgtcaaaggt
     8281 gcttgcccat tacgataaaa ttctcgccat tcttctcttg ccacacggta gtagttcatt
     8341 ggatcattca tattagcctc ctgcgttctc ccatcaatgg aactatttta acacaaaata
     8401 aacgcaaacc ccaccactta agagagattg ccgatctttt tgtataatct ttataataga
     8461 aagaaacgga gtcttattct tgacttgcgc acggatacat aaaaggatag actgttgtca
     8521 tgaaagaaat gagaaggatg gaagacatga caaatcatta ctattctgaa cgcccagata
     8581 cggcgcacga ttttgaacaa tgggcttttg agttaaaagg cagaaccttt caatttgtaa
     8641 cagattctgg tgtattttca cgtgaaacag tggattatgg ttcacgtgtc ctgattgatg
     8701 ctttcgattg ggaagagtta cctgatgaag gaaagatctt agatgtggga tgcgggtatg
     8761 gaccgattgg gttagctttg gcatttgcga cacagcggtt tgttgaaatg gtcgacatta
     8821 atgaacgagc ggtaagtcta gcgcaaggaa acgcaaagcg taaccaaatc gaacatgttg
     8881 atatccatca atcgaatatc tatgaagcag ttcatgaaga aacctatgca gctatcgtaa
     8941 gcaatccgcc gatccgtgct ggaaaaaaag ttgtacatga aattttgaca ggcgcatatc
     9001 ctcggttgaa gaagggcggt acactgacaa ttgtgatcca gaagaaacaa ggagcgccga
     9061 gtgcgcaaaa gaaaatggaa gaaacatttg gtaatgcaga aatcgtcaca aaagataaag
     9121 gatattatat catcaaaagt gtgaaagaat aactagctga aaaatagtgg agcgacgaaa
     9181 gagtggaact gggcgtgcat gtacgggtac gtatcagttc tttttttgtt gagaagtttt
     9241 taaaaacaaa gaactatttt ttcttcaaaa agaaatagta ggaacagagt cgttgttttt
     9301 tacgataaaa taaataagat acagaacttg tatagaaggc tgccaagtcg gtagataaag
     9361 gaggttttta tcaaaaaaca tactttcctt ttttctctat tcttttcttg ggggttttgg
     9421 tacactgata gggaatttag atagaaggga gccaatagga tgaagctgac catcagagat
     9481 atcgcagaaa tggcaggcgt atcagtaaca actgtctcac agattttgaa taacaaagga
     9541 agcagattta gtgataagac aagaaaaaaa gtgttgtcga tcgtgaatga acatcattat
     9601 aagcccgatt atttcgcttc aaatgtgatc aatcgacatt caaagaccat cggaatgatc
     9661 gtccctgatg tcactgattt cttcttctcc aaactgatcg aaggtgtaga gagctatttg
     9721 aatcctttgg gctatgtgat catgttatgc aattctcgtc atagccaaga aaatgagatc
     9781 aagtatttac aagagctttc ccaccgctca gtggacggaa tcttgctggc cacaccaaat
     9841 attttacctg agaaatatgc attagacagc ggtttttatc aaaaaatgcc aatcatttta
     9901 atcgatcgcg gattgaatcg tcgggataac ggacggctta tcgtaaagga atatgagggc
     9961 gcctatcaag ctgtttcgct gtttattgaa aatggccata cgaagatcgg catgctgaag
    10021 gaaaccactg gatactatca attagaagaa cgtttcaacg gatatcgtca tgcgctgaaa
    10081 gatcatggcc tgccttttaa tgggaaattt gttgaacatg gagagttgac tgtccaaggt
    10141 ggatatactg catcaaaaaa attactcaaa cataaagata tcacggctat tttttgcggg
    10201 aatgatgcca tggcgatcgg atgctatcag gcgatcgatg aattgggcaa aaaaatccca
    10261 gaagatatct cagtcatagg attcgatgga ttaaagctgt cagaatacat gattccgcaa
    10321 ttgacgacag tgcagcagcc aagttttgat attggatttt atgctgcacg tttcctgatc
    10381 gatacgatcg aatttcctca aagaaaagtt ccgaacaaag tgttcgaaac aaaattaatt
    10441 attcgtgaga gcgtaaaagt attgacaaag ggctaactcg gcggtatatt gcttatgtaa
    10501 gcatttacaa aggctagtta gtgctttttt tatttttagt aaaccagttt actaaagtgg
    10561 tttacctttc ctaaaaataa tgtataatac cgaatgaaga caaggaggat caggcaatgc
    10621 gaatggtgga tctgattgaa aaaaagaggg atggaaacga gctgtcgaaa gaggaaatcg
    10681 agtacatcgt cacgaactat acgaatggaa agattccaga ttatcaagtc agtgcattat
    10741 tgatggcaat tttttatcaa gacatgacaa atgaagaaat cacgaactta acattggcga
    10801 ttgctaattc tggtgatgtc atcgatttga gttctcttga aggaataaag gtagataagc
    10861 attcgacagg tggcgttggt gacacgacca cattgatttt agccccccta gtagctagtg
    10921 tcggagtgac agtggctaaa atgtctggac gcggtctagg ttacacaggt ggtacactag
    10981 acaagctaga agcaattccc ggatttcaga tcgagctttc ggatgaagaa tttgtacgca
    11041 ttgttaatga aagtaaagta gcggtaattg gtcaatcagg aaacttggca ccggcggaca
    11101 aaaaattgta tgccttgcgt gacgtcacag cgaccgttga ttctcttccg ctgattgcca
    11161 gttcgattat gagtaaaaaa atcgcggcag gagcagatgc aatcgttttg gatgtgacca
    11221 ctggagacgg tgctttcatg aagaacatcg aagatgcaag acgtctggca aagaccatga
    11281 cgagcattgg gaaactggct aaccgagaaa cagtggcggt tatctcagat atgtcagaac
    11341 ctcttggcga agctatcgga aatagtctag aagttgtcga ggcaatcgaa acattacaag
    11401 gaaatggtcc agaagatcta gtagaaatgt gctacgcctt aggaagccaa atggttgttc
    11461 ttgctggcaa agctaagaca accgatgaag caagaacgtt gcttcaagaa gctctggaat
    11521 caggtaaagc gttagcaaaa ttcaaagaaa tgatccaaaa tcaaggggga gacccagcga
    11581 tcgtggaaca tccagaacga attttgactg cacgctacac aatggagctt cctgctaaac
    11641 aaagcggagt tgtgtcaaaa atcgtggcaa atgaattagg aattgctgca atgatgctag
    11701 gtgctgggcg taaaaccaaa gaagaagata ttgatcacgc tgttggattg aaactgcata
    11761 aaaaaattgg tgacactgtg acaaaaggtg agtcactatt gacgatctat agtaacgacg
    11821 aagaaatcac ttctgtcatt gaattattat ataaaaatat tgaaattggt gaatcggcaa
    11881 tgaagccaac gttgattcat gacatcatca cagaataaag gaaaaaagcg gactttaccg
    11941 ttttaaagaa aaggggaaaa gaaatggaac taaaccgaat gatcgaccat actattttga
    12001 aagcagacgc atctaaagaa gctgtgatgc aaattatcga agaagcaaaa aaataccatt
    12061 tttattctgt ctgcatcaat ccagcatggg ttgcgctagc gaaagaacaa ttacaaggtg
    12121 agccagtagc cgtatgtaca gtaatcgggt ttcctctagg agcaaacaca cccgagacaa
    12181 aagcatttga aacaacagat gccatcaata acggtgcaga tgaagtcgat atggtcatca
    12241 atatcggtgc tttgaaatca aaggatgatc aacaagtcca aaaagatatc gaagcagttg
    12301 ttgaagcagc aaaagatcgt gcgcttgtca aagttattat tgaaacatcg ttattagata
    12361 gagaagaaat catccgagct tgcgaaatag caaaagctgc aggcgctgat tttgtcaaaa
    12421 catcaactgg tttctcaaca ggcggagcaa aggtagagga tgtccgtttg atgcgtgaaa
    12481 ctgtaggacc agaaatgggt gtcaaagctt ccggcggtat ccataacgaa gaagaagcaa
    12541 tggcaatgat cgaagctggt gctacaagaa tcggaacaag cgcaggagta gcaatcgtat
    12601 ctggttcaac tggtgaaggc tattaatttt ttcaaaggag aagctcatgg cagaactcaa
    12661 acaagaatgg atcaacacag ctattgaagc gttagataaa gcctatgttc cttattccca
    12721 ttttccggta ggcgcttgtc tcgtaacgga atcaggcaag atttatcaag gcatcaacat
    12781 tgagaatgca tcatttggat taacgaattg tgccgagcgc acagcttttt tcaaagcagt
    12841 atctgaagga gaaaaaacat ttacgcatct agtcgtagcg gggcacaccc ccgatcctat
    12901 ctctccttgt ggtgcatgtc gacaagtaat ggcagaattc tgttcccctg atatgccggt
    12961 tacgctagta ggagacaatg gtgtgacgaa ggcaacgacc gttcgtgcac tgctgcctta
    13021 tgcatttact gaaaaagatc tataactcat gttcattcag tcagctgact gttgaaaaat
    13081 aaaacaatta ttttaggagg ctttacagaa atgaaaaaag caaaattatt tggtttaggt
    13141 gctgtcgcac tagcagcagg gttattttta ggtgcatgcg gcaacaacgg ctctactgat
    13201 tcatccggtg gcaaaagctc atcagacaca acaactgcag cattgatcac agacacaggt
    13261 ggtgtagacg accgttcatt caaccagtca gcttgggaag gactagaaaa atgggggaaa
    13321 gatcaaggac tttcacgagg aaatgatggc ttccaatatt tccaatcttc aaatgaatca
    13381 gattacatcc caaatattga ccaagcattg aatgctggat tcaaaacgat ttttggtatt
    13441 ggttataaac taaaaccagc gatccaagaa caagcaacaa acaatacagg tacgaacttt
    13501 gttattatcg atgatgtcat cgatggatta gataacgttg tatctgcaac tttcaaagac
    13561 aacgaagctt cttatcttgc aggtgttgca gcagcttata cgactgaaac aaacgtagtc
    13621 ggctttatcg gcggtgtaaa aggtgaagta atcgatcgtt ttgatgctgg attcaaagcc
    13681 ggtgttgatg caggtgcgaa agaattagga aaagaaatca aagtattgaa tcaatatgct
    13741 ggtgacttct ctgctccaga taaaggacgt tcaattgctc aaggtatgta tgcacaaaac
    13801 gcagatatca tcttccatgc ttcaggcggt acaggtaacg gtgtattcca agaagcaaaa
    13861 tcattgaacg aatcaggcga caaaaaagtt tgggttatcg gtgttgaccg cgaccaatca
    13921 gacgaaggcg aatacacatt aaacggcgaa aagaaaaact tcacattgac atctacactg
    13981 aaagcagttg gaacagtagt agaagatttg gcacaaaaat cagcggacgg taaattccct
    14041 ggcggcgaac acactgttta tggattgaaa gaagacggtg ttggtctaac agaaggacaa
    14101 ctttctgacg aagcgaaaaa agcagttgat gaagcaaaag aaaaaatcat ttcaggtgac
    14161 gtaaaagttc cagaaactcc tgaagaaaac taaaaccatt tctatagcct tgttagacac
    14221 atgtttgaca aggctatggt ttgattagaa caagaattgt tcatttatcc aagctgcctt
    14281 gtgacagttt ggataaggga acaactccct gaagtaatgt ttgtttcaaa agaagaacgc
    14341 tcactaagcg tgaatcttag tcaatcgttg ccctagatag aaatcgttag gatgtgaaaa
    14401 aaaggtggtt caagaaaata ttgtcataga aatgcagaat attaccaaag aatttggaac
    14461 attcaaggca aatgacaaca tcaatttaca agtaaaggca ggagaaattc atgcgttgct
    14521 tggagaaaac ggtgctggca aatctacatt gatgaacgtg ctttccggat tattagagcc
    14581 gacatcaggg aaaattttga tgcgtgggaa agaagtaaag atcacaagtc cgaccaaagc
    14641 caaccaatta gggattggga tggtccatca gcactttatg cttgttgatg cctttactgt
    14701 aacagaaaac atcgtgttgg gaagcgagcc tagtcgcgca gggatgcttg accataaaaa
    14761 agcacgaaaa gagatccaaa aagtttctga acagtatgga ttgtcagtca acccagatgc
    14821 ttacgttcgt gatatttcag ttggaatgga acaacgggta gaaattttaa aaacacttta
    14881 ccgaggagca gatgtactga tttttgatga gccgacagct gtattgaccc ctcaagaaat
    14941 tgatgaatta atcgtgatca tgaaggaatt agtcaaagaa ggcaagtcga tcattttgat
    15001 tacgcataag ttagatgaaa tcaaagcagt agctgaccgc tgtacagtta tccgccgtgg
    15061 aaaaggaatc ggtacagtca acgttaaaga cgtaacctca caacaattag ccgatatgat
    15121 ggttggaaga acggtttcat tcaaaacgat gaaaaaagaa gcgaagcctc aagaagtcgt
    15181 tttgtctatt gaaaatctag tggtgaaaga aaatcgtgga ttagaagccg tgaaaaactt
    15241 gaacttagag gttcgtgctg gcgaagtact tggtatcgct ggaatcgatg gaaacgggca
    15301 gtcggagttg atccaagctt tgactggttt gcgaaaggca gaaagcggac atatcaagct
    15361 aaaaggggaa gacatcacta ataaaaaacc ccgaaaaatc actgaacatg gtgtaggaca
    15421 cgtgccagaa gaccgtcata aatacgggtt agtcctagat atgacattat ctgaaaatat
    15481 cgcactacaa acctatcatc aaaaacctta cagtaaaaac ggcatgctga attattcagt
    15541 aatgaatgaa catgccagag aattgatcga agaatatgat gttcgaacaa cgaatgaact
    15601 tgttcccgca aaagctttat caggcggaaa tcagcaaaaa gcaattatcg ctcgtgaagt
    15661 cgaccgagat cctgatctgt tgattgttgc aaatccaact cgtggactgg atgtaggtgc
    15721 gatcgaattt attcataaac gtctgatcga acaaagggac aaatacaaag cagtgttact
    15781 gatcagtttc gaattagaag aaattttaaa tgtttcggat cgtattgctg ttatccatga
    15841 aggagaaatt gtcggtatcg ttgatccgaa agaaacatct gaaaatgaat taggtctatt
    15901 gatggcgggc tataccttag aagaagcacg aaaagagtta gaaaaggggg ctggcgcaca
    15961 tgaatgatcg cagcgaaaaa ttccgtaatt tattagtgcc tgtactttca gttgttttag
    16021 gattgatctt aggcgcgata ttgatggctg cttttggttt tcagcccatc caaggttatt
    16081 cttctatgct agatgcagcc cttggttcgc aaagagggat aggtgaaacg ttaaggcaag
    16141 caacgccgtt gatttttaca gcattaggtt tttctgtagc taactcagct gggtttttca
    16201 atattgggct ttctggacaa gcactttgcg ggtggatcgt cagtgtctgg gtggcacttg
    16261 cgtttccaga tctgccaaaa gttgttttgc ttccgttatg cgtgatctta ggtgccttag
    16321 ctggtgcgat ggcagcggct gttccaggtt tacttcgagc attttttgga acaagtgaag
    16381 tgatcgtaac aatcatgatg aactatatct tattatacgt aagcacattt gctttacatg
    16441 aagtgatgcc tgaaagctat cgctcaagtt tggactcttc caatatgatc agtcaaaatg
    16501 cctcactgcg gttaccatgg ttaacacaag cttttggcgg ctctcgcgta aactcaggat
    16561 tatttttagc aatcatcgcg cttattttcg tttggctgct gatgaagaaa acgactttag
    16621 gatttgaaat ccgttctgtt ggattgaatc cttttgcttc tgagtatgca ggaatgagta
    16681 gtaaacgtac gatcgttttg tcgatggtca tttctggcgc tttggcaggg cttggcggtg
    16741 tggttgaagg attaggaacg tatcaaaact tctttgttca aacgacttcg ctatctattg
    16801 gatttgacgg aatggctgtt tccttgcttg gttctggatc agcaatcggt attcttttat
    16861 cagcgatctt gttcagtatt ttgaagatcg gtggacttgg catgcagaca ggagcaggtg
    16921 ttccgtttga gatcgtcaat gtatcgattg ctttgattat cttctttgtc gggatcaact
    16981 tcctgattcg tttcgtcatg gctaaagtct tccaagggaa aaaacaagaa gagatcgttg
    17041 cgacaataga atcgaaacca acgaatcaaa accatgaagg gggcgaacta taatgtcgac
    17101 tatcgaatta attgccttga tccttacttc tacactggtt tattcaacac cgctaattct
    17161 aacgtcacta ggcggaactt tttctgaacg aagcgggatc gtaaacgtag ggctagaggg
    17221 aatcatggtc atgggtgctt ttagttccat cgttttcaac ttgacgatga gtccatcact
    17281 tggttctatg acaccatgga tcgctatttt agtgggtggc gtcgtaggta tcctattttc
    17341 tctgattcac gcagtagcaa cagtcagttt gcgtgctgac catattatca gcgggacagt
    17401 aattaatctg atggcacctg ctttaggtgt tttcttgatc aaagcatggt atggtaaagg
    17461 tcaaactgat aacatcacgc agaatctggg gtatttttct ttcccaggat tagcagatat
    17521 tccggtcatt ggaccaattt tctttaaaaa tacattctta cctgcttatt gtgctatttt
    17581 gattgctgtc ctagcttggg tgatcatctt caagacgaaa ttcggcttgc gcctccgttc
    17641 agttggtgaa aatcctcaag ctgccgacac attaggtatc aatgtatatg ctatgcgtta
    17701 ttcaggcgta ctgatttctg gcttgttagg cggtatgggc ggtgctgttt ttgctcagtc
    17761 gatttccggt aacttttcag ctggtacgat cgttggacaa gggttcatct ccatggctgc
    17821 tatgatcttt ggtaagtgga atcctttagg ggcaatgggt gcctcattgt tctttggttt
    17881 tgctcaaagc ttgagtatca ttggcgcaca attaccagtt atttcttcga ttccgccagt
    17941 attgctgcaa attgctccat atttgctgac aatcattgtg ctggttgttt tcttagggaa
    18001 agcttctggt ccaaaagcaa acgggaagaa ctatatcaaa tcaaaataat cttttagcga
    18061 ataaaaaggg tgggttacgg gaagtgtttt tcgtctcgcc ctttttgttt taaaggcaat
    18121 aaaaataaaa agaaagaagg aataatcatg tttaaacgcg tacatctgat cgtcatggat
    18181 tcagtaggaa ttggagaagc accagatgcc gataagttcg gtgatgttgg ttctgataca
    18241 ttaggacaca ttgcgaaaga agcaggattg aacgttccac atttagagca gcttggttta
    18301 ggaacgatcc gtcctcttga aggtgtggaa tcagtgaaag atcatcaagg ctatgcgacg
    18361 aaattagaag aagtttctgt agggaaagat acgatgacag gccactggga aatcatggga
    18421 ttgaatatca aaactccatt tcgtgtgttt cctaatggat tccctgatga tctgatcaaa
    18481 caaatcgaag aattttctgg ccgtaaagtc gtatgtaaca aaccttatag cggaacagct
    18541 gttattgacg attacggtga acaccaaatg aaaacaggcg atttaattgt atatacttct
    18601 gccgatcctg tccttcagat cgcggcacat gaagaaatca ttcctttaga agaattatat
    18661 cggatttgcc aattcgttcg tgatataaca aaagatgatc cgtatatgat tggccgaatc
    18721 attgcacgtc cgtacgtggg tgaaccagga gacttcacac gaacaagcaa tcgccatgat
    18781 tatgcgttga atccattcgg acacacagtc cttgattctt tgaaagaagc aggaaaggac
    18841 gtgattgctg ttggaaagat caacgatatt ttcaacggac aaggaatcac tgaatctgtc
    18901 cgcacgaaaa gcaatatgga tggcgttgac cagctgctga aagttatgaa aaacgatttt
    18961 tcaggaatca gctttaccaa tttagtagat tttgatgcat tatacggtca tcgtcgggat
    19021 gtagtcggct atgcgcatgc gattgaagaa tttgacttac gaattccaga attgcttgaa
    19081 gcgatggaag aagacgactt gctaatgatt acagccgatc acggaaacga tccgacgttt
    19141 actggtaccg atcatacgcg agaatatgta ccgttattgg tttacagtaa aaaaatgaaa
    19201 gaacaagggg cattgcctca aggatattat tcagatattt cagcaacgat tgctgagaac
    19261 tttagtgttt cttcgactga aaatggcgaa agcttcttga aattattaaa ataatctttt
    19321 taaataggag aaagcaaatg accaagttaa gcgagatgtt acaagaaacg acccagttta
    19381 tccttgataa aggagtaaaa gaaattgatt ttggtttgat tctaggttca ggattaggtg
    19441 aattagcaga tgaaatcgaa gatgctgtaa tgatttctta tgatgagatc ccgttctttc
    19501 ctgtttcgac agtagttggg catgcaggaa agctagttta tgggtctctg gcaggcaaga
    19561 aagtgttagc catgcaaggg agattccact actatgaagg tcattccatg caaacagtga
    19621 cctatccagt acgtgtgatg gccggactag gggcccattc agtcattgtg actaatgcat
    19681 gtggtggtgt caacgaatca tttgtaccag gagatttgat gctgattacc gatcatatca
    19741 attttactgg tgaaaatcct ttgatcggtc cgaatgaaga tgcaatgggc cctagatttc
    19801 cagatatgag tcaagcttat acgcctgact atcaagcaac agcaaaagca gcagcagaaa
    19861 aactaggttt ggatcttaaa gaaggcgtat atatgggcta ttctggacca acttatgaaa
    19921 cgcctgcgga gatccgaatg tctcggacaa taggagccga tgcagtaggg atgtcgactg
    19981 ttccagaagt catcgtagca gctcacagcg gattaaaagt gttaggaatc tcttgtatta
    20041 ccaacttagc agcaggtatg caagcgaatc tgagccatga agaagttgta gaaacaactc
    20101 agagagtcaa acaatctttt aaagcattgg ttaaagaaac attgacatta ttgtaatagg
    20161 aggaacaata aaatgagtgt gcatattgaa gcgaaagaag gcgagatcgc cgataagatt
    20221 ttacttccgg gagatccttt gcgagctaaa tatattgcgg agactttttt ggaagatcca
    20281 gtttgttaca atcaggtacg cggtatgtta ggctttaccg gcaaatataa aggagaacgt
    20341 atttctgttc aaggaacagg aatgggaatg ccatcagcca ccatctatgc ccatgaattg
    20401 atccagtcgt atggtgtaaa gaaattgatt cgagtaggta catgcggtgc attgtcaaaa
    20461 gacgtccatg ttcgtgattt agtactagca caaggtgcag cgaccagttc ttcgatgatc
    20521 gaaaaaaatt tccaagcctt tcattttcct ccaatcagtg atttcaatct attactgaaa
    20581 gcatatgaga tagctaaaga aaagggctat acggttcatg taggaaatgt tctttcggaa
    20641 gattctttct acaaagatga tttgacagag acgttccaat tagctgaact tggcgtttta
    20701 ggagtggaaa tggaagcagc ggctctttat tacttaggcg cgaaatacca tgtgcagaca
    20761 ttaagtttga tgaccgttag cgaccatttg atcaccggtg aagaaacaac agctgcagaa
    20821 cgtcaatcta cgttcaatga aatgatcgaa gtaggattgg aaacagcgat agcagaataa
    20881 gaaggaaatg aaaaagctgc cctgcattaa gtaataagaa catccaagca aaaatagctc
    20941 ctcatgtttt tgacttggct ggacttatta ttgcaaatgt aagcttttga acaccgttta
    21001 ccaagaggtt gtgaatctgc cgtttaactc tgagtcacaa ggaacaattt caaaaa
//
LOCUS       DASHRQ010000207        21056 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.guided.048, whole genome shotgun sequence.
ACCESSION   DASHRQ010000207 DASHRQ010000000
VERSION     DASHRQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.guided.048"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="U2M88_002964"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="U2M88_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEM8375257.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="U2M88_002965"
     CDS             2039..2227
                     /locus_tag="U2M88_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEM8375258.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..2638)
                     /locus_tag="U2M88_002966"
     CDS             complement(2285..2638)
                     /locus_tag="U2M88_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8375259.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            3169..3591
                     /locus_tag="U2M88_002967"
     CDS             3169..3591
                     /locus_tag="U2M88_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM8375260.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            3588..3818
                     /locus_tag="U2M88_002968"
     CDS             3588..3818
                     /locus_tag="U2M88_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM8375261.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            4316..4516
                     /locus_tag="U2M88_002969"
     CDS             4316..4516
                     /locus_tag="U2M88_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEM8375262.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            4543..5736
                     /locus_tag="U2M88_002970"
     CDS             4543..5736
                     /locus_tag="U2M88_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM8375263.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5804..7369)
                     /gene="guaA"
                     /locus_tag="U2M88_002971"
     CDS             complement(5804..7369)
                     /gene="guaA"
                     /locus_tag="U2M88_002971"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEM8375264.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(7428..8351)
                     /locus_tag="U2M88_002972"
     CDS             complement(7428..8351)
                     /locus_tag="U2M88_002972"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEM8375265.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            8547..9152
                     /locus_tag="U2M88_002973"
     CDS             8547..9152
                     /locus_tag="U2M88_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM8375266.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            9460..10476
                     /locus_tag="U2M88_002974"
     CDS             9460..10476
                     /locus_tag="U2M88_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEM8375267.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            10617..11918
                     /locus_tag="U2M88_002975"
     CDS             10617..11918
                     /locus_tag="U2M88_002975"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEM8375268.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11964..12626
                     /gene="deoC"
                     /locus_tag="U2M88_002976"
     CDS             11964..12626
                     /gene="deoC"
                     /locus_tag="U2M88_002976"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEM8375269.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            12647..13045
                     /locus_tag="U2M88_002977"
     CDS             12647..13045
                     /locus_tag="U2M88_002977"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEM8375270.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            13111..14193
                     /locus_tag="U2M88_002978"
     CDS             13111..14193
                     /locus_tag="U2M88_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM8375271.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            14405..15967
                     /locus_tag="U2M88_002979"
     CDS             14405..15967
                     /locus_tag="U2M88_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM8375272.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15960..17093
                     /locus_tag="U2M88_002980"
     CDS             15960..17093
                     /locus_tag="U2M88_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8375273.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            17093..18049
                     /locus_tag="U2M88_002981"
     CDS             17093..18049
                     /locus_tag="U2M88_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM8375274.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            18148..19314
                     /gene="deoB"
                     /locus_tag="U2M88_002982"
     CDS             18148..19314
                     /gene="deoB"
                     /locus_tag="U2M88_002982"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEM8375275.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            19338..20156
                     /locus_tag="U2M88_002983"
     CDS             19338..20156
                     /locus_tag="U2M88_002983"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEM8375276.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            20173..20880
                     /gene="deoD"
                     /locus_tag="U2M88_002984"
     CDS             20173..20880
                     /gene="deoD"
                     /locus_tag="U2M88_002984"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEM8375277.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         6783 a         3752 c         4511 g         6010 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgttctctg
      661 ttccctcttt atcatggaag tgcaaaaagt aatataggga ttgataacct tatagaagtt
      721 attactaata aattttattc atcaacacat cgaggtccgt ctgaactttg cggaaatgtt
      781 ttcaaaattg aatatacaaa aaaaagacaa cgtcttgcat atatacgcct ttatagtgga
      841 gtactacatt tacgagattc ggttagagta tcagaaaaag aaaaaataaa agttacagaa
      901 atgtatactt caataaatgg tgaattatgt aagattgata gagcttattc tggagaaatt
      961 gttattttgc aaaatgagtt tttgaagtta aatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt cgagtgcttt
     2401 taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt aatgatacaa
     2461 gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt gttaaataac
     2521 gtgggtcaat ttcaatcatt gttcccactt gttcacgagt gagaccccgt ttcattcgag
     2581 cctctttaat cgctaaacca acggctctaa aatcgtattt atcttctttt ttacgcatac
     2641 taaatcacct ctatacattt tactgttcct atataaccat aaacaggtat agaaaaacat
     2701 gttatatagt ttatgagttc ctatagaaca ctgtggaatt tattaatgcg atgaatccaa
     2761 tttcaaatag aaaggtcgct gattttattt acctatacaa ctttataaaa tttagtcata
     2821 ctcctagaaa gtagataaaa ttggcgactt aatggaggga aaagtcgcca tgagaaagat
     2881 aatctgtcat ctagttctct taattaagtt cttatcaaaa aaacttatcc acctgtactg
     2941 gatagatatg accatcaatg tggttttgca gattacatag atagaaaaat caaagcatat
     3001 gaacagagaa aacaatctgt ttatgtgctt ttttggtcac ttaataaata tcttttagga
     3061 tttctcttca tttatgcaac aaatccccct ttcgtgtcgg ggtttagtat gaagggagat
     3121 aaagaagcac atgccactcc ctttcacgac agaaaggggg tgaaaagcat gaaaccatct
     3181 tcttttcaag aaaccataga aaatcagttt gactatatct gcaaacaagt tattgaagat
     3241 gaacgaaaag attacttcaa acatctagac agactatcag caaaagaagt ctcgttttct
     3301 gatatagata actaccttgt caactctttt tctactgttg atacgtacgt cactgatttc
     3361 caactattta aattatttgg attaagaatc aatgttgaaa gtgatttatt aagtgaagcc
     3421 ctgcgtcatc tttcagagaa aaaacggaac attattttac ttcattactt tatggatatg
     3481 agtgatgtgg agattgcgga gttactaaat ttgaatcgtt cgactgttta tcgtcatcga
     3541 ataagtggac tagccatgat taaagaattc atgaaggagt gtaaagaatg aaaacagctt
     3601 atccaagggt tcccttcccc ctcattgtaa aggcgacaga tggcgacgtg gaagcaatta
     3661 atcagattgt taaacattat cgagggtata cctcaaaacg ttcattacgt cgcatgacag
     3721 acgaatatgg aaattctcat atggttattg atgaaaccct acgtggcaga atggaaacga
     3781 aactcatctc taagatttta tcgttcgaaa ttaaatagtc ccattttttc gtggaagcgt
     3841 gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt attccatcaa agcatattgt
     3901 gtgttcagta ggttttgata ggctaacaag ccaaacgttc tttgacaacc aaataaaggc
     3961 aatcgaatac gtttcggtaa gaaaagagct gatgaactaa cacgccatga cctacaaaat
     4021 tagtgagcga ttcatgttag taattcgaaa gtaatcttta gcaggattgc tgccatgact
     4081 ttcttatcgt gataatgata ctcccgtata gccatagtcc gagcgttaga agcgtcacag
     4141 gcaatgggta cggctacatg agaaccatgc agaagtgaaa ttcctgtgag ctttgctaga
     4201 gctgttcgat tgctaagata ttcttctctt gtcagtgtat tattataaaa ctatttgata
     4261 taattatagg tgaatattgg agttttccta gcctataatt gggaaaggag taataatgat
     4321 aacggtcaca aaaaaggatt tagttgagct aggatatgga acttcttttt cagcagacat
     4381 cataaaaaaa gcaaaagaat tgatgattac aaaagggcat atctactatc aatctcgaaa
     4441 gctagataga gtccctcgtg aagctgttga agaaattcta ggaattcggt tttcaaatga
     4501 aaacaggtct gagtagtttt tgcacagtaa ggagtaaaaa ctatggttaa agacccaata
     4561 aaaaaagcaa agaatggaac ctattatttt cgtgcaaatc taggttatga ttctaaaggg
     4621 aaaaaaattc aaaaatatcg tagtggattc actactaaaa aagaagcaag agaagaatat
     4681 tcgaaactgc tattgatgaa accagaagaa ttaagtgaga ataaggataa aatgacgttt
     4741 aaacattata tctttgaaat tttccttcca tggtataaga cacaggtaaa gctcagaaca
     4801 tatgaaaatc gcttaccaac tattaagaag catttctctt attttgataa aatggctgtt
     4861 tccgatattg agcctattga tgtgcaaaat tggcaactaa aattatcaaa gaagtgtaag
     4921 tcttcttatg taagagctgt tcaaggacta ttttcagttg ctatggatcg agcaattgtc
     4981 cttgggataa ccaccacaaa tccatctaag attattggca atgtgaaaaa gcaaaagtct
     5041 aaaattgaat tttggacaaa agaagaattt gaaaaagtaa tctctctaat ttacaaagaa
     5101 gattactatc aacacttttt atttatctct ctgtggtttt tatttatgac tggaatgcga
     5161 attggtgaag ctactgccat tcaatgggaa gatattgatt ttgactcagg agtactaacc
     5221 atcaacaaaa cactctatta caagaatcaa tcgaattata gatttgttga accaaaaaca
     5281 aaggcgagcg ttcgtcatat tgtattagat aaatgcacat taacttattt atccgaatgg
     5341 aaggaagtac aacaaaatct aattcagaca gactttgtaa tgagctataa cggtattcct
     5401 acccaaaaac atacactatc atatgctatt gaacgctatg ctaagctggc tggtatccat
     5461 cgaatcagaa tccatgggtt acggcattct catgcttcct tgttaatcag tatgggagaa
     5521 aatccactta tcattaaaga tcgtctagga cacgaagata ttgagacgac tttaggaacg
     5581 tatggacatc tttatcctaa cagtaatttc gaggtagcta acaaattaaa tggagccatt
     5641 tcctttaaag aagcaaaaca aaattatgat acttcaccaa agaatcaatt tactgtagac
     5701 tacttgcgaa acaaagaaat gaaaaaagtg caataaaagt gcagtaaaaa gataagaaga
     5761 gccagaaacc cttggtataa gaggatttct agctctccta caactattcc cactccactg
     5821 ttgctggtgg tttagaagtg atgtcataca cgatacggtt gacgtgggct acttcgttta
     5881 cgatacgtac agagattttt tgcaagactt cccatgggat acgggcaaag tcagcggtca
     5941 ttccgtcgat tgaagttaca gcacggatac cgactgtgta gtcgtaagta cgtccgtcac
     6001 ccataacacc gacagagcgg atccctggaa gaacagtgaa atattgccag atatcacggt
     6061 caagtccagc agctgcgatt tcttcacgca agatagcatc agattcgcgt acgatttcta
     6121 gcttgtcttc agtgatttcg ccaagaacac gaataccaag accaggacca gggaatggct
     6181 gacgccaaac gattgaatca ggcatgccta actgagtccc taattcacgg acttcgtctt
     6241 tgaataatgt gtttaatggt tcgatcaatt caaattgcat atcttctggc agtccaccta
     6301 cattatggtg agatttgatc gtctgtgctg tttctgtacc agattcgata acgtcagtat
     6361 acaacgttcc ttgtgctagg aaagagacgc cttcttcacc agcaagtttt gttgcttcgt
     6421 catcaaaaac atatacgaat tcattcccaa tgattttacg tttttgttct gggtcagata
     6481 cacctttcaa cttgtctaag aagcgatctc ttgcatcgac tttgatgatg ttcaagccga
     6541 attttccgcc tagactttcc attacttgtt ccgcttcgcc tttgcgtagc agaccgtggt
     6601 caacaaagat agatgtcaat tggtcaccga tcgctttttg taaaagaacc ccaacgacac
     6661 tagaatcaac gccacctgaa agaccaagca agactttttt gtcaccaact tgttcacgga
     6721 ttttttctac ttgcatatcg ataaagttat ccattgtcca gttaccttca cagtgacaaa
     6781 catcaaaggc aaagtggcgc agcaattcga ttccgtattc ggaatgacgt acttctgcat
     6841 ggaattggat accataaaaa ttacgtgttg tgtcttggat agaagcaatc ggacaatctt
     6901 tgcttgttcc gactgtttca aagccttcag gaactttagt taccaagtca ccatggctca
     6961 tccatactgt ttgttgtttt ggtgtttctt taaacaagac agcttgatcg cttgttactt
     7021 ctaacaaggc tttcccatat tcgcggtttt tagcaggctc tacggaaccg ccaagtttgt
     7081 acgtgatcaa ttgcatacca tagcaaatcc ctaatacagg aatacctaaa ttgaagatct
     7141 caggatcgat cccaaatgca ttctcatcat atacactgtt tggtccgcca gaaagaatga
     7201 ttccctttgg attcatcgct tttacttctt ctgcagtaat gcggtggctc aataattcgg
     7261 aaaagactcc aagatcacga atacgtcttg tgatcaattg gttatactga ctaccgtagt
     7321 ctaatacgat aatcttttca acgttcgtca tatcggcaac gtttgtcaaa tttattaccc
     7381 ctatcccttt aaattttatc tcaaaatagt caaactattt taaaatgcta atattttcgc
     7441 atgaaaatcg aatcgatgat atgattttca gttttatgga gaattatatc ggctcttcct
     7501 cgtgtaggca agatatattc ctgtaaattt ttcaaattca catttttcca tacatctttt
     7561 gccattttaa aggcttcttc acgatctccg atagcaaaac gataataata attctctgga
     7621 ttttggaaag ccgtatccag taacgcacca aaacgatcca aataccattt ttcgatcagc
     7681 acgggatcgg catcgacata gacagaaaaa tcaaaaaagt cgctgacgta gatttgctga
     7741 ttcgcaggta attgaagcgt attgatcccc tcaacgatca agatatcagg ctgctggatc
     7801 aattcgtact tgtctggaat gatatcatac acatcatgtg agtaaacagg tacttttatc
     7861 tcttcctgac cagattttac acggttcaag aaatcgatca gcatctccat atcataactt
     7921 tcaggaaatc ctttacggtc catgattcca cgctctttta atatacgatt tggataaaga
     7981 aatccgtcag tcgtgatcag ctgaacgttt cttcgtttga aggtacgaga caaaatcgtc
     8041 tgcagcagtc tggctgttgt actttttcca acggctacac ttccggcgat cccgataata
     8101 aaaggcggta cttctacgta tttatgaaga aataaacctt tactcaagaa caaagattca
     8161 aattctttca tataaagata aatcagatga gcaagaggta catagatatc tcgaacatcc
     8221 tgcatggaaa tgcggtcatt caaacttttg atattatcta gttcatcttg tgtcaaaggt
     8281 gcttgcccat tacgataaaa ttctcgccat tcttctcttg ccacacggta gtagttcatt
     8341 ggatcattca tattagcctc ctgcgttctc ccatcaatgg aactatttta acacaaaata
     8401 aacgcaaacc ccaccactta agagagattg ccgatctttt tgtataatct ttataataga
     8461 aagaaacgga gtcttattct tgacttgcgc acggatacat aaaaggatag actgttgtca
     8521 tgaaagaaat gagaaggatg gaagacatga caaatcatta ctattctgaa cgcccagata
     8581 cggcgcacga ttttgaacaa tgggcttttg agttaaaagg cagaaccttt caatttgtaa
     8641 cagattctgg tgtattttca cgtgaaacag tggattatgg ttcacgtgtc ctgattgatg
     8701 ctttcgattg ggaagagtta cctgatgaag gaaagatctt agatgtggga tgcgggtatg
     8761 gaccgattgg gttagctttg gcatttgcga cacagcggtt tgttgaaatg gtcgacatta
     8821 atgaacgagc ggtaagtcta gcgcaaggaa acgcaaagcg taaccaaatc gaacatgttg
     8881 atatccatca atcgaatatc tatgaagcag ttcatgaaga aacctatgca gctatcgtaa
     8941 gcaatccgcc gatccgtgct ggaaaaaaag ttgtacatga aattttgaca ggcgcatatc
     9001 ctcggttgaa gaagggcggt acactgacaa ttgtgatcca gaagaaacaa ggagcgccga
     9061 gtgcgcaaaa gaaaatggaa gaaacatttg gtaatgcaga aatcgtcaca aaagataaag
     9121 gatattatat catcaaaagt gtgaaagaat aactagctga aaaatagtgg agcgacgaaa
     9181 gagtggaact gggcgtgcat gtacgggtac gtatcagttc tttttttgtt gagaagtttt
     9241 taaaaacaaa gaactatttt ttcttcaaaa agaaatagta ggaacagagt cgttgttttt
     9301 tacgataaaa taaataagat acagaacttg tatagaaggc tgccaagtcg gtagataaag
     9361 gaggttttta tcaaaaaaca tactttcctt ttttctctat tcttttcttg ggggttttgg
     9421 tacactgata gggaatttag atagaaggga gccaatagga tgaagctgac catcagagat
     9481 atcgcagaaa tggcaggcgt atcagtaaca actgtctcac agattttgaa taacaaagga
     9541 agcagattta gtgataagac aagaaaaaaa gtgttgtcga tcgtgaatga acatcattat
     9601 aagcccgatt atttcgcttc aaatgtgatc aatcgacatt caaagaccat cggaatgatc
     9661 gtccctgatg tcactgattt cttcttctcc aaactgatcg aaggtgtaga gagctatttg
     9721 aatcctttgg gctatgtgat catgttatgc aattctcgtc atagccaaga aaatgagatc
     9781 aagtatttac aagagctttc ccaccgctca gtggacggaa tcttgctggc cacaccaaat
     9841 attttacctg agaaatatgc attagacagc ggtttttatc aaaaaatgcc aatcatttta
     9901 atcgatcgcg gattgaatcg tcgggataac ggacggctta tcgtaaagga atatgagggc
     9961 gcctatcaag ctgtttcgct gtttattgaa aatggccata cgaagatcgg catgctgaag
    10021 gaaaccactg gatactatca attagaagaa cgtttcaacg gatatcgtca tgcgctgaaa
    10081 gatcatggcc tgccttttaa tgggaaattt gttgaacatg gagagttgac tgtccaaggt
    10141 ggatatactg catcaaaaaa attactcaaa cataaagata tcacggctat tttttgcggg
    10201 aatgatgcca tggcgatcgg atgctatcag gcgatcgatg aattgggcaa aaaaatccca
    10261 gaagatatct cagtcatagg attcgatgga ttaaagctgt cagaatacat gattccgcaa
    10321 ttgacgacag tgcagcagcc aagttttgat attggatttt atgctgcacg tttcctgatc
    10381 gatacgatcg aatttcctca aagaaaagtt ccgaacaaag tgttcgaaac aaaattaatt
    10441 attcgtgaga gcgtaaaagt attgacaaag ggctaactcg gcggtatatt gcttatgtaa
    10501 gcatttacaa aggctagtta gtgctttttt tatttttagt aaaccagttt actaaagtgg
    10561 tttacctttc ctaaaaataa tgtataatac cgaatgaaga caaggaggat caggcaatgc
    10621 gaatggtgga tctgattgaa aaaaagaggg atggaaacga gctgtcgaaa gaggaaatcg
    10681 agtacatcgt cacgaactat acgaatggaa agattccaga ttatcaagtc agtgcattat
    10741 tgatggcaat tttttatcaa gacatgacaa atgaagaaat cacgaactta acattggcga
    10801 ttgctaattc tggtgatgtc atcgatttga gttctcttga aggaataaag gtagataagc
    10861 attcgacagg tggcgttggt gacacgacca cattgatttt agccccccta gtagctagtg
    10921 tcggagtgac agtggctaaa atgtctggac gcggtctagg ttacacaggt ggtacactag
    10981 acaagctaga agcaattccc ggatttcaga tcgagctttc ggatgaagaa tttgtacgca
    11041 ttgttaatga aagtaaagta gcggtaattg gtcaatcagg aaacttggca ccggcggaca
    11101 aaaaattgta tgccttgcgt gacgtcacag cgaccgttga ttctcttccg ctgattgcca
    11161 gttcgattat gagtaaaaaa atcgcggcag gagcagatgc aatcgttttg gatgtgacca
    11221 ctggagacgg tgctttcatg aagaacatcg aagatgcaag acgtctggca aagaccatga
    11281 cgagcattgg gaaactggct aaccgagaaa cagtggcggt tatctcagat atgtcagaac
    11341 ctcttggcga agctatcgga aatagtctag aagttgtcga ggcaatcgaa acattacaag
    11401 gaaatggtcc agaagatcta gtagaaatgt gctacgcctt aggaagccaa atggttgttc
    11461 ttgctggcaa agctaagaca accgatgaag caagaacgtt gcttcaagaa gctctggaat
    11521 caggtaaagc gttagcaaaa ttcaaagaaa tgatccaaaa tcaaggggga gacccagcga
    11581 tcgtggaaca tccagaacga attttgactg cacgctacac aatggagctt cctgctaaac
    11641 aaagcggagt tgtgtcaaaa atcgtggcaa atgaattagg aattgctgca atgatgctag
    11701 gtgctgggcg taaaaccaaa gaagaagata ttgatcacgc tgttggattg aaactgcata
    11761 aaaaaattgg tgacactgtg acaaaaggtg agtcactatt gacgatctat agtaacgacg
    11821 aagaaatcac ttctgtcatt gaattattat ataaaaatat tgaaattggt gaatcggcaa
    11881 tgaagccaac gttgattcat gacatcatca cagaataaag gaaaaaagcg gactttaccg
    11941 ttttaaagaa aaggggaaaa gaaatggaac taaaccgaat gatcgaccat actattttga
    12001 aagcagacgc atctaaagaa gctgtgatgc aaattatcga agaagcaaaa aaataccatt
    12061 tttattctgt ctgcatcaat ccagcatggg ttgcgctagc gaaagaacaa ttacaaggtg
    12121 agccagtagc cgtatgtaca gtaatcgggt ttcctctagg agcaaacaca cccgagacaa
    12181 aagcatttga aacaacagat gccatcaata acggtgcaga tgaagtcgat atggtcatca
    12241 atatcggtgc tttgaaatca aaggatgatc aacaagtcca aaaagatatc gaagcagttg
    12301 ttgaagcagc aaaagatcgt gcgcttgtca aagttattat tgaaacatcg ttattagata
    12361 gagaagaaat catccgagct tgcgaaatag caaaagctgc aggcgctgat tttgtcaaaa
    12421 catcaactgg tttctcaaca ggcggagcaa aggtagagga tgtccgtttg atgcgtgaaa
    12481 ctgtaggacc agaaatgggt gtcaaagctt ccggcggtat ccataacgaa gaagaagcaa
    12541 tggcaatgat cgaagctggt gctacaagaa tcggaacaag cgcaggagta gcaatcgtat
    12601 ctggttcaac tggtgaaggc tattaatttt ttcaaaggag aagctcatgg cagaactcaa
    12661 acaagaatgg atcaacacag ctattgaagc gttagataaa gcctatgttc cttattccca
    12721 ttttccggta ggcgcttgtc tcgtaacgga atcaggcaag atttatcaag gcatcaacat
    12781 tgagaatgca tcatttggat taacgaattg tgccgagcgc acagcttttt tcaaagcagt
    12841 atctgaagga gaaaaaacat ttacgcatct agtcgtagcg gggcacaccc ccgatcctat
    12901 ctctccttgt ggtgcatgtc gacaagtaat ggcagaattc tgttcccctg atatgccggt
    12961 tacgctagta ggagacaatg gtgtgacgaa ggcaacgacc gttcgtgcac tgctgcctta
    13021 tgcatttact gaaaaagatc tataactcat gttcattcag tcagctgact gttgaaaaat
    13081 aaaacaatta ttttaggagg ctttacagaa atgaaaaaag caaaattatt tggtttaggt
    13141 gctgtcgcac tagcagcagg gttattttta ggtgcatgcg gcaacaacgg ctctactgat
    13201 tcatccggtg gcaaaagctc atcagacaca acaactgcag cattgatcac agacacaggt
    13261 ggtgtagacg accgttcatt caaccagtca gcttgggaag gactagaaaa atgggggaaa
    13321 gatcaaggac tttcacgagg aaatgatggc ttccaatatt tccaatcttc aaatgaatca
    13381 gattacatcc caaatattga ccaagcattg aatgctggat tcaaaacgat ttttggtatt
    13441 ggttataaac taaaaccagc gatccaagaa caagcaacaa acaatacagg tacgaacttt
    13501 gttattatcg atgatgtcat cgatggatta gataacgttg tatctgcaac tttcaaagac
    13561 aacgaagctt cttatcttgc aggtgttgca gcagcttata cgactgaaac aaacgtagtc
    13621 ggctttatcg gcggtgtaaa aggtgaagta atcgatcgtt ttgatgctgg attcaaagcc
    13681 ggtgttgatg caggtgcgaa agaattagga aaagaaatca aagtattgaa tcaatatgct
    13741 ggtgacttct ctgctccaga taaaggacgt tcaattgctc aaggtatgta tgcacaaaac
    13801 gcagatatca tcttccatgc ttcaggcggt acaggtaacg gtgtattcca agaagcaaaa
    13861 tcattgaacg aatcaggcga caaaaaagtt tgggttatcg gtgttgaccg cgaccaatca
    13921 gacgaaggcg aatacacatt aaacggcgaa aagaaaaact tcacattgac atctacactg
    13981 aaagcagttg gaacagtagt agaagatttg gcacaaaaat cagcggacgg taaattccct
    14041 ggcggcgaac acactgttta tggattgaaa gaagacggtg ttggtctaac agaaggacaa
    14101 ctttctgacg aagcgaaaaa agcagttgat gaagcaaaag aaaaaatcat ttcaggtgac
    14161 gtaaaagttc cagaaactcc tgaagaaaac taaaaccatt tctatagcct tgttagacac
    14221 atgtttgaca aggctatggt ttgattagaa caagaattgt tcatttatcc aagctgcctt
    14281 gtgacagttt ggataaggga acaactccct gaagtaatgt ttgtttcaaa agaagaacgc
    14341 tcactaagcg tgaatcttag tcaatcgttg ccctagatag aaatcgttag gatgtgaaaa
    14401 aaaggtggtt caagaaaata ttgtcataga aatgcagaat attaccaaag aatttggaac
    14461 attcaaggca aatgacaaca tcaatttaca agtaaaggca ggagaaattc atgcgttgct
    14521 tggagaaaac ggtgctggca aatctacatt gatgaacgtg ctttccggat tattagagcc
    14581 gacatcaggg aaaattttga tgcgtgggaa agaagtaaag atcacaagtc cgaccaaagc
    14641 caaccaatta gggattggga tggtccatca gcactttatg cttgttgatg cctttactgt
    14701 aacagaaaac atcgtgttgg gaagcgagcc tagtcgcgca gggatgcttg accataaaaa
    14761 agcacgaaaa gagatccaaa aagtttctga acagtatgga ttgtcagtca acccagatgc
    14821 ttacgttcgt gatatttcag ttggaatgga acaacgggta gaaattttaa aaacacttta
    14881 ccgaggagca gatgtactga tttttgatga gccgacagct gtattgaccc ctcaagaaat
    14941 tgatgaatta atcgtgatca tgaaggaatt agtcaaagaa ggcaagtcga tcattttgat
    15001 tacgcataag ttagatgaaa tcaaagcagt agctgaccgc tgtacagtta tccgccgtgg
    15061 aaaaggaatc ggtacagtca acgttaaaga cgtaacctca caacaattag ccgatatgat
    15121 ggttggaaga acggtttcat tcaaaacgat gaaaaaagaa gcgaagcctc aagaagtcgt
    15181 tttgtctatt gaaaatctag tggtgaaaga aaatcgtgga ttagaagccg tgaaaaactt
    15241 gaacttagag gttcgtgctg gcgaagtact tggtatcgct ggaatcgatg gaaacgggca
    15301 gtcggagttg atccaagctt tgactggttt gcgaaaggca gaaagcggac atatcaagct
    15361 aaaaggggaa gacatcacta ataaaaaacc ccgaaaaatc actgaacatg gtgtaggaca
    15421 cgtgccagaa gaccgtcata aatacgggtt agtcctagat atgacattat ctgaaaatat
    15481 cgcactacaa acctatcatc aaaaacctta cagtaaaaac ggcatgctga attattcagt
    15541 aatgaatgaa catgccagag aattgatcga agaatatgat gttcgaacaa cgaatgaact
    15601 tgttcccgca aaagctttat caggcggaaa tcagcaaaaa gcaattatcg ctcgtgaagt
    15661 cgaccgagat cctgatctgt tgattgttgc aaatccaact cgtggactgg atgtaggtgc
    15721 gatcgaattt attcataaac gtctgatcga acaaagggac aaatacaaag cagtgttact
    15781 gatcagtttc gaattagaag aaattttaaa tgtttcggat cgtattgctg ttatccatga
    15841 aggagaaatt gtcggtatcg ttgatccgaa agaaacatct gaaaatgaat taggtctatt
    15901 gatggcgggc tataccttag aagaagcacg aaaagagtta gaaaaggggg ctggcgcaca
    15961 tgaatgatcg cagcgaaaaa ttccgtaatt tattagtgcc tgtactttca gttgttttag
    16021 gattgatctt aggcgcgata ttgatggctg cttttggttt tcagcccatc caaggttatt
    16081 cttctatgct agatgcagcc cttggttcgc aaagagggat aggtgaaacg ttaaggcaag
    16141 caacgccgtt gatttttaca gcattaggtt tttctgtagc taactcagct gggtttttca
    16201 atattgggct ttctggacaa gcactttgcg ggtggatcgt cagtgtctgg gtggcacttg
    16261 cgtttccaga tctgccaaaa gttgttttgc ttccgttatg cgtgatctta ggtgccttag
    16321 ctggtgcgat ggcagcggct gttccaggtt tacttcgagc attttttgga acaagtgaag
    16381 tgatcgtaac aatcatgatg aactatatct tattatacgt aagcacattt gctttacatg
    16441 aagtgatgcc tgaaagctat cgctcaagtt tggactcttc caatatgatc agtcaaaatg
    16501 cctcactgcg gttaccatgg ttaacacaag cttttggcgg ctctcgcgta aactcaggat
    16561 tatttttagc aatcatcgcg cttattttcg tttggctgct gatgaagaaa acgactttag
    16621 gatttgaaat ccgttctgtt ggattgaatc cttttgcttc tgagtatgca ggaatgagta
    16681 gtaaacgtac gatcgttttg tcgatggtca tttctggcgc tttggcaggg cttggcggtg
    16741 tggttgaagg attaggaacg tatcaaaact tctttgttca aacgacttcg ctatctattg
    16801 gatttgacgg aatggctgtt tccttgcttg gttctggatc agcaatcggt attcttttat
    16861 cagcgatctt gttcagtatt ttgaagatcg gtggacttgg catgcagaca ggagcaggtg
    16921 ttccgtttga gatcgtcaat gtatcgattg ctttgattat cttctttgtc gggatcaact
    16981 tcctgattcg tttcgtcatg gctaaagtct tccaagggaa aaaacaagaa gagatcgttg
    17041 cgacaataga atcgaaacca acgaatcaaa accatgaagg gggcgaacta taatgtcgac
    17101 tatcgaatta attgccttga tccttacttc tacactggtt tattcaacac cgctaattct
    17161 aacgtcacta ggcggaactt tttctgaacg aagcgggatc gtaaacgtag ggctagaggg
    17221 aatcatggtc atgggtgctt ttagttccat cgttttcaac ttgacgatga gtccatcact
    17281 tggttctatg acaccatgga tcgctatttt agtgggtggc gtcgtaggta tcctattttc
    17341 tctgattcac gcagtagcaa cagtcagttt gcgtgctgac catattatca gcgggacagt
    17401 aattaatctg atggcacctg ctttaggtgt tttcttgatc aaagcatggt atggtaaagg
    17461 tcaaactgat aacatcacgc agaatctggg gtatttttct ttcccaggat tagcagatat
    17521 tccggtcatt ggaccaattt tctttaaaaa tacattctta cctgcttatt gtgctatttt
    17581 gattgctgtc ctagcttggg tgatcatctt caagacgaaa ttcggcttgc gcctccgttc
    17641 agttggtgaa aatcctcaag ctgccgacac attaggtatc aatgtatatg ctatgcgtta
    17701 ttcaggcgta ctgatttctg gcttgttagg cggtatgggc ggtgctgttt ttgctcagtc
    17761 gatttccggt aacttttcag ctggtacgat cgttggacaa gggttcatct ccatggctgc
    17821 tatgatcttt ggtaagtgga atcctttagg ggcaatgggt gcctcattgt tctttggttt
    17881 tgctcaaagc ttgagtatca ttggcgcaca attaccagtt atttcttcga ttccgccagt
    17941 attgctgcaa attgctccat atttgctgac aatcattgtg ctggttgttt tcttagggaa
    18001 agcttctggt ccaaaagcaa acgggaagaa ctatatcaaa tcaaaataat cttttagcga
    18061 ataaaaaggg tgggttacgg gaagtgtttt tcgtctcgcc ctttttgttt taaaggcaat
    18121 aaaaataaaa agaaagaagg aataatcatg tttaaacgcg tacatctgat cgtcatggat
    18181 tcagtaggaa ttggagaagc accagatgcc gataagttcg gtgatgttgg ttctgataca
    18241 ttaggacaca ttgcgaaaga agcaggattg aacgttccac atttagagca gcttggttta
    18301 ggaacgatcc gtcctcttga aggtgtggaa tcagtgaaag atcatcaagg ctatgcgacg
    18361 aaattagaag aagtttctgt agggaaagat acgatgacag gccactggga aatcatggga
    18421 ttgaatatca aaactccatt tcgtgtgttt cctaatggat tccctgatga tctgatcaaa
    18481 caaatcgaag aattttctgg ccgtaaagtc gtatgtaaca aaccttatag cggaacagct
    18541 gttattgacg attacggtga acaccaaatg aaaacaggcg atttaattgt atatacttct
    18601 gccgatcctg tccttcagat cgcggcacat gaagaaatca ttcctttaga agaattatat
    18661 cggatttgcc aattcgttcg tgatataaca aaagatgatc cgtatatgat tggccgaatc
    18721 attgcacgtc cgtacgtggg tgaaccagga gacttcacac gaacaagcaa tcgccatgat
    18781 tatgcgttga atccattcgg acacacagtc cttgattctt tgaaagaagc aggaaaggac
    18841 gtgattgctg ttggaaagat caacgatatt ttcaacggac aaggaatcac tgaatctgtc
    18901 cgcacgaaaa gcaatatgga tggcgttgac cagctgctga aagttatgaa aaacgatttt
    18961 tcaggaatca gctttaccaa tttagtagat tttgatgcat tatacggtca tcgtcgggat
    19021 gtagtcggct atgcgcatgc gattgaagaa tttgacttac gaattccaga attgcttgaa
    19081 gcgatggaag aagacgactt gctaatgatt acagccgatc acggaaacga tccgacgttt
    19141 actggtaccg atcatacgcg agaatatgta ccgttattgg tttacagtaa aaaaatgaaa
    19201 gaacaagggg cattgcctca aggatattat tcagatattt cagcaacgat tgctgagaac
    19261 tttagtgttt cttcgactga aaatggcgaa agcttcttga aattattaaa ataatctttt
    19321 taaataggag aaagcaaatg accaagttaa gcgagatgtt acaagaaacg acccagttta
    19381 tccttgataa aggagtaaaa gaaattgatt ttggtttgat tctaggttca ggattaggtg
    19441 aattagcaga tgaaatcgaa gatgctgtaa tgatttctta tgatgagatc ccgttctttc
    19501 ctgtttcgac agtagttggg catgcaggaa agctagttta tgggtctctg gcaggcaaga
    19561 aagtgttagc catgcaaggg agattccact actatgaagg tcattccatg caaacagtga
    19621 cctatccagt acgtgtgatg gccggactag gggcccattc agtcattgtg actaatgcat
    19681 gtggtggtgt caacgaatca tttgtaccag gagatttgat gctgattacc gatcatatca
    19741 attttactgg tgaaaatcct ttgatcggtc cgaatgaaga tgcaatgggc cctagatttc
    19801 cagatatgag tcaagcttat acgcctgact atcaagcaac agcaaaagca gcagcagaaa
    19861 aactaggttt ggatcttaaa gaaggcgtat atatgggcta ttctggacca acttatgaaa
    19921 cgcctgcgga gatccgaatg tctcggacaa taggagccga tgcagtaggg atgtcgactg
    19981 ttccagaagt catcgtagca gctcacagcg gattaaaagt gttaggaatc tcttgtatta
    20041 ccaacttagc agcaggtatg caagcgaatc tgagccatga agaagttgta gaaacaactc
    20101 agagagtcaa acaatctttt aaagcattgg ttaaagaaac attgacatta ttgtaatagg
    20161 aggaacaata aaatgagtgt gcatattgaa gcgaaagaag gcgagatcgc cgataagatt
    20221 ttacttccgg gagatccttt gcgagctaaa tatattgcgg agactttttt ggaagatcca
    20281 gtttgttaca atcaggtacg cggtatgtta ggctttaccg gcaaatataa aggagaacgt
    20341 atttctgttc aaggaacagg aatgggaatg ccatcagcca ccatctatgc ccatgaattg
    20401 atccagtcgt atggtgtaaa gaaattgatt cgagtaggta catgcggtgc attgtcaaaa
    20461 gacgtccatg ttcgtgattt agtactagca caaggtgcag cgaccagttc ttcgatgatc
    20521 gaaaaaaatt tccaagcctt tcattttcct ccaatcagtg atttcaatct attactgaaa
    20581 gcatatgaga tagctaaaga aaagggctat acggttcatg taggaaatgt tctttcggaa
    20641 gattctttct acaaagatga tttgacagag acgttccaat tagctgaact tggcgtttta
    20701 ggagtggaaa tggaagcagc ggctctttat tacttaggcg cgaaatacca tgtgcagaca
    20761 ttaagtttga tgaccgttag cgaccatttg atcaccggtg aagaaacaac agctgcagaa
    20821 cgtcaatcta cgttcaatga aatgatcgaa gtaggattgg aaacagcgat agcagaataa
    20881 gaaggaaatg aaaaagctgc cctgcattaa gtaataagaa catccaagca aaaatagctc
    20941 ctcatgtttt tgacttggct ggacttatta ttgcaaatgt aagcttttga acaccgttta
    21001 ccaagaggtt gtgaatctgc cgtttaactc tgagtcacaa ggaacaattt caaaaa
//
LOCUS       DASHRQ010000208        20985 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.guided.049, whole genome shotgun sequence.
ACCESSION   DASHRQ010000208 DASHRQ010000000
VERSION     DASHRQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.guided.049"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(134..577)
                     /locus_tag="U2M88_002985"
     CDS             complement(134..577)
                     /locus_tag="U2M88_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEM8375278.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="U2M88_002986"
     CDS             complement(795..2135)
                     /locus_tag="U2M88_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8375279.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="U2M88_002987"
     CDS             2404..3300
                     /locus_tag="U2M88_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEM8375280.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="U2M88_002988"
     CDS             complement(3450..4391)
                     /locus_tag="U2M88_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEM8375281.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="U2M88_002989"
     CDS             4517..5638
                     /locus_tag="U2M88_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEM8375282.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="U2M88_002990"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="U2M88_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEM8375283.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="U2M88_002991"
     CDS             7426..7992
                     /locus_tag="U2M88_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEM8375284.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="U2M88_002992"
     CDS             8203..9264
                     /locus_tag="U2M88_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEM8375285.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="U2M88_002993"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="U2M88_002993"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEM8375286.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="U2M88_002994"
     CDS             10177..12159
                     /locus_tag="U2M88_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM8375287.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="U2M88_002995"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="U2M88_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEM8375288.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="U2M88_002996"
     CDS             12881..14305
                     /locus_tag="U2M88_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM8375289.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="U2M88_002997"
     CDS             complement(14486..15259)
                     /locus_tag="U2M88_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEM8375290.1"
                     /translation="MTLLLKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="U2M88_002998"
     CDS             complement(15256..16161)
                     /locus_tag="U2M88_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM8375291.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGVGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="U2M88_002999"
     CDS             complement(16315..16635)
                     /locus_tag="U2M88_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEM8375292.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17109..17795
                     /locus_tag="U2M88_003000"
     CDS             17109..17795
                     /locus_tag="U2M88_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEM8375293.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17797..18741
                     /locus_tag="U2M88_003001"
     CDS             17797..18741
                     /locus_tag="U2M88_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEM8375294.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18940..19584
                     /gene="pcp"
                     /locus_tag="U2M88_003002"
     CDS             18940..19584
                     /gene="pcp"
                     /locus_tag="U2M88_003002"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEM8375295.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19663..20940
                     /locus_tag="U2M88_003003"
     CDS             19663..20940
                     /locus_tag="U2M88_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375296.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6781 a         3783 c         4360 g         6061 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt acttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttaaaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc caacgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    16981 aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa
    17041 acaactatat tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag
    17101 gaaaatatat ggaaaattat cttagccttt taggcgtctt gattgttatt ctcgggtttg
    17161 catttaaatt agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg
    17221 gcggtcttgg cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg
    17281 ggatggcgat ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga
    17341 aagaatcagc ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag
    17401 atatttatgg gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg
    17461 ctggatttgt tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt
    17521 tagaagtcca tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg
    17581 tctgctggtt ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat
    17641 caacactgaa agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc
    17701 cagtggctat cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat
    17761 tagcgaagaa atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat
    17821 attttattag gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt
    17881 tacactggag taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt
    17941 ttctggacgg tcttaggggt agttattgca ggaggaagat ggcttccggc aatcgtgaac
    18001 ggtggcttga tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa
    18061 agccggcttc catcaaaaga atatatggaa aaaatgtcag acaaactagg aatgaaaatt
    18121 tttattccag ctttgagtat cggtgtcttt gcgatcttgt ttgctctatt taccaattta
    18181 ggtgctttag tcggcgtggg cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt
    18241 tctcgagata atcgaccaag tacattttta gatgatgcag cagatatgct gggcacagta
    18301 gggccgttaa gcatgttgcc aatgctttta gcttctctag gtgcggtatt tacaagcgct
    18361 ggagttggga cagtgatttc caatgcagta ggtacgatcg ttccagaagg aaatgtgaac
    18421 atcggcatca tcatttacgc tcttggcatg gtgatattta cggtaatcat ggggaatgca
    18481 tttgcggcca tcaccgtgat gacggttggg atcggtgcac cattcgtatt tagtcatgga
    18541 gcgaatccag cgttaatagg aatggttgca ttgacttgcg gtttttgtgg gaccctgctg
    18601 acaccgatgg ctgctaactt caatatcgtg cctgtagcga tgctggagat gaaagacaaa
    18661 tacggagtga tcaaaaacca gttgtttatt gcgttattta tgctagtttt ccaaattgct
    18721 tatatgattc tatttaaata atagaagaga ggggttgtga cagaataatt gtcgcaacct
    18781 cttctaacat tgtagtggaa cactattttt ttataagaat atattaagtt tgatctgtta
    18841 tgttatttaa actattgttt cctttattta cgctgttcaa ggaaaatggt atgataaaag
    18901 ataaccatat gttaaatcaa tcggacggag gaacaacaga tgaaagtact agtaacaggt
    18961 tttgatccat ttggtgggga caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca
    19021 gatgaaatca gcggtgctga aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc
    19081 agtcaagtag tcaaagaagc catccaacag caccaaccag atgtcgtgat ctgtgtcgga
    19141 caagcaggtg ggcggtcagc agtctcgttc gaacgtgtcg caatcaatct agcagaagca
    19201 agaatccctg ataatgaagg aaatcagcca ttcgatacgg ctttagaaga aaacggtcca
    19261 gccgcttact ttacctcatt gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt
    19321 ctaccagcgt acatctctta tacagcagga acatttgtct gcaatgatat catgtatcgt
    19381 ttgcttcatt tgatcgaaac acaattcccg gcaattcgag gtgggttcat ccacgtgcca
    19441 ttctcacccg atcaagtgat cgatcgtcca gtgggtacgc cttctatgtc tttagaagac
    19501 attgcttctt ctttgactta tgcagttgaa gcagcagtag aaaataaaga agatatttcc
    19561 ggaaatgccg gaacaactca ctaatttagc aagaaacact ggctggttgc taacgttatg
    19621 ttagtagcca gctttttgta attagaaagg aaagaaacag ccgtggaaga atggcaatcc
    19681 gtctttgaag aatggtttcc aaaagaaatc agcaaaagct atcccatcaa aatttctaaa
    19741 caatacacaa gcagtcaacg ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa
    19801 ctagtcgata agcaccgccg atatctaatc agctctcgtt ttatggaaga acattattta
    19861 gctgcaacag attgggtgtt ttctgacttc aagatcaatc cgttttttcg aacaaaacga
    19921 agccagcaaa aattgtattg tgagtgtggt agagaattaa aagtgcaata catcgttaaa
    19981 tcgccaaaaa ccggaaagat cctaaaacta ggcatcaacc actttgccga tcacctgcat
    20041 gtctctccga cagtggcagc ttcgatccat cagggaatga caaaagttga tctggcctta
    20101 gacgaattat tatggcttaa gcagaagaat atcgattttc ctgaaggact atggcaaaaa
    20161 tactgttttg tcttgtacca aaatcgtcgg atgaaacagc catatttacc ggatataaaa
    20221 ttagctcagc ggctcgcaga atttcgccaa gtagaaatgc cgatttacat agcggattat
    20281 caagcattgg aaaatgaaat caagaaaata agtgaacaca tcaatgggca gtcaaaaaag
    20341 cgacagataa aaaaagaact atttgacgac tttgcagaag aattagtaaa ggatgtagaa
    20401 gaattcttga tcaactatcg cgcattttta cgtaaagatt ggcaatccat cgtatatgaa
    20461 gaagtgccag tacatcctaa tgcttatttt gaaacattta tttccgtgtt gagaaagaca
    20521 aaacgtcaac gaacaccaga agtcactgct cagatggaat attttgcgaa aaaccaacga
    20581 ttcatccaac caaaaatcta tctatttatt tggaagcaat attgtcgcta tggttttaca
    20641 gaagggttct tcgacagcat tcctcggata gtcagaaatg gttttttaaa agtattgcgt
    20701 aaagaacgag aagctataca atctgctgat aagaaagacc gtacagtctc aaaagaaaaa
    20761 tggcaactag tagtgaaaga tatccaatct ggaaatgtgc aagagacaat cgacaaatgg
    20821 aaaggaaaac attatcgttt tactgaagct cagaaacaag cgttggaata ttatcagaag
    20881 ttagaagaat cactgcgttt caatgatgag gcaagaaagt atctgaagga attactataa
    20941 aaaatagagg ctgggacaga agcgtttaac tccaagaaat aagaa
//
LOCUS       DASHRQ010000209        16774 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.guided.062, whole genome shotgun sequence.
ACCESSION   DASHRQ010000209 DASHRQ010000000
VERSION     DASHRQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.guided.062"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            366..1229
                     /locus_tag="U2M88_003004"
     CDS             366..1229
                     /locus_tag="U2M88_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEM8375297.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            1329..1811
                     /locus_tag="U2M88_003005"
     CDS             1329..1811
                     /locus_tag="U2M88_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEM8375298.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(2074..2541)
                     /locus_tag="U2M88_003006"
     CDS             complement(2074..2541)
                     /locus_tag="U2M88_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEM8375299.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            2664..3515
                     /locus_tag="U2M88_003007"
     CDS             2664..3515
                     /locus_tag="U2M88_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM8375300.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(3766..5723)
                     /locus_tag="U2M88_003008"
                     /pseudo
     CDS             complement(3766..5723)
                     /locus_tag="U2M88_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            complement(5720..6592)
                     /locus_tag="U2M88_003009"
     CDS             complement(5720..6592)
                     /locus_tag="U2M88_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEM8375301.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(6934..7806)
                     /locus_tag="U2M88_003010"
     CDS             complement(6934..7806)
                     /locus_tag="U2M88_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEM8375302.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            7934..8503
                     /locus_tag="U2M88_003011"
     CDS             7934..8503
                     /locus_tag="U2M88_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEM8375303.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8570..9307
                     /locus_tag="U2M88_003012"
     CDS             8570..9307
                     /locus_tag="U2M88_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM8375304.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            9538..10806
                     /locus_tag="U2M88_003013"
     CDS             9538..10806
                     /locus_tag="U2M88_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEM8375305.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            11293..11757
                     /locus_tag="U2M88_003014"
     CDS             11293..11757
                     /locus_tag="U2M88_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM8375306.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            11741..12370
                     /locus_tag="U2M88_003015"
     CDS             11741..12370
                     /locus_tag="U2M88_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEM8375307.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            12724..14202
                     /gene="msr(C)"
                     /locus_tag="U2M88_003016"
     CDS             12724..14202
                     /gene="msr(C)"
                     /locus_tag="U2M88_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEM8375308.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            14249..14695
                     /locus_tag="U2M88_003017"
     CDS             14249..14695
                     /locus_tag="U2M88_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEM8375309.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(14894..15409)
                     /locus_tag="U2M88_003018"
     CDS             complement(14894..15409)
                     /locus_tag="U2M88_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEM8375310.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            15526..16728
                     /locus_tag="U2M88_003019"
     CDS             15526..16728
                     /locus_tag="U2M88_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM8375311.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         5300 a         2987 c         3482 g         5005 t
ORIGIN      
        1 gatgaggttg aacttttaca caagattgtg gacttgactg caaagaaaaa tccaagcagc
       61 gttctgatca gcagtttgaa caacaatttg ctttgtacag taggtagtaa gcggattaac
      121 taagggaata aaagatattt ttaccgcttt gtttacttag aatctgtctt ccccaaaatt
      181 tatttttcta aaggtaaaac atagataggc agataatacc aattatccgt tacgaaacgc
      241 tgcttgagaa ttttctttag atccatacaa cattttctac atctgactaa attggttcct
      301 gattcatcaa gtaattgact ttctttgata atatgataaa aaggcaatca aggaggaaga
      361 taaatatgct taaagatctg aaccaattga tcgattacat cgaagaacat ttgacagaag
      421 gactttccta taaaataata gccgaaaata caggaatctc tgaatatcaa ttaaaacgca
      481 tctttacgtt tattgcaggg atgtcgctaa tggactatat caaaaaccgt agattggctt
      541 tggctaatcg cgatcttcta gatggatcaa gagtgacaga aacggcgttt agatatggat
      601 atgaaacagt cgaaggtttt tcaagagctt ttcgtgattg gtcaggatat ctgccttcag
      661 aaatcagcag atacggcata caaaaatctt ttccacgctt gacatttttt attgatgtca
      721 gaggaggaaa atcgatggaa tttcgtatcg aaaagaaaga acagttttat gtggcaggtg
      781 tggcgaaaaa agttcctgtc aaatttgaag gagtcaatca agaaatacaa aagttagcgc
      841 agacgatcac ggagactcaa agggaagaga tgcataagtt aggagattta tatccaaatc
      901 aagtaatcaa cgtttcttat ggatttgatg gagaccgctt ggaagaaaag ggcagtttga
      961 tgcacatgat tggctttgct acgacaaaag aaaatccgta cgctgattta gaaatgcttg
     1021 ctatagaaga acacacttgg gccatttttc ctaataaagg gccatttccc caaactttac
     1081 aagatacttg ggggaaaatc tatgcagaat ggcttccttc ttcagattat gggttagtcg
     1141 aagctcccga gatctctttt acaaaatggg gagaggattt cagtaatgtt tacagtgaga
     1201 tttggattgc agtgaaagca aaaaaatagt taaaaaataa aaatatccct agaaaaaaga
     1261 gcgtaatcgt gtcatttccc tttaatttta tgtaagatga acttaatata atagaaaggg
     1321 tgggagaaat ggcaaagtat aggatcgaag aaaaaccaga ttttacggtg acgggtattg
     1381 gtgtggaatt gaagagtagc aatacggatt ttgtcggttt ggctgcagag aaacaagcat
     1441 ttgagcaaaa agttatcggt aatggaacaa tagatgcatt gaaacaagct gctgaaaatc
     1501 attatttctt tacagtcaac gaaccctata acgggaaaat gatgtactat gtcggtgttc
     1561 aaacgagtca ggatgttcca aacactacgc gaatcatccc attcccaaaa ggaacgtatc
     1621 ttgttgtaag cgctttagca gaatcgcctg aagaattaag aaatgatttg acgaatacgg
     1681 cctttgggca tgttttaaga gaagtagaag attacgccta tgttgggggt ccaaatgctg
     1741 tagtcgaaat gggagttacc aacgggaaat atatgggaga aatctggata ccggtagtca
     1801 ataaaccatg ataatataga ggtcgtgaaa gtagcgcttt gacctgagca ttagagagga
     1861 aaaatgggaa atagctttcc atattttgca tttttcagaa ctaatgccga gggtcagcta
     1921 cttgaacacc gtttatcaag acgtcatgac ttcagcgctt tgacctgagc attagagagg
     1981 aaaaaggggc atgacaaggc ttttgtcccc cggagaataa aagaatttat gtagacgatt
     2041 ccgatcagaa aaatgcaggc acagtagaga gaattataaa aatctctcta cgaggcctgt
     2101 gttttctggt cttttttctt tttccaaaac ggctatttta tgtatcagac tatcaagtgt
     2161 gatcttagcc atttcttctt ctgccttttt ctgcagccga gcgtagcttt cttctaaagc
     2221 ttcttggata ttcgcaccaa cgacgcaatt tggatttgtt tttggatcga cttggataag
     2281 ctgtgtatta ccttcgatgc tgcgatagac atctagtaaa gaaatctgat ctggactttt
     2341 ggcaagggaa ggctgcggtt ttccggtttt tgtaaagatc aaaccggatt ttttgagctg
     2401 actcatgatt ttacgtacat ttgctgcatt tgtttcgata ctactggcaa tcatctcact
     2461 agaaagtgat cgggtatctt gaaacatttc aatataggca agtacatgga ttgcatcact
     2521 aaattgcaga gaatacttca tcagttgtca tcctttcatt ttttttgctt gacttatctt
     2581 accacactct ttatgataaa ggtgtaattt ataaatgtac agctgtaaat cttaacaaat
     2641 aaaaagaata gggagtggca aatatgcatt atttagtaac aggagcaact ggaggttttg
     2701 gtggttatgc gctggaattc ttaaaaaaga tggttccaca aaatgaaatc tatgtactag
     2761 tacgtagtga agaaaaagga gaagcactga aagaagctgg atttaatata cgtattggtg
     2821 attatgatga tttagactca atgaaagaag cgttgaaagg aatagatcga ttgctttttg
     2881 tctctggcgt tccaggaaat cgtcaagccg aacataagaa tgtagtgaag gcagcacaag
     2941 cagctggggt atcttatatc gcttatacaa gttttgcaga tgccgatcac tcgatcagtg
     3001 tattagcgcc agatcaccaa ttcactgaaa aaatgattaa ggattcaggt atttcccata
     3061 catttttacg gaataattgg tatgtagaga atgaattacc tatcattggg caggctttaa
     3121 aaactggtca attcgtttat gctgcaggaa atggaaaaac aggttgggca ttgaaacgtg
     3181 aatatgcaga agctgcagca aaagtactag tagaaagtga ctctccagaa atcttagaat
     3241 tatccggcaa tctaactgac tatgaagaat tagccaaggc attagagcgt gcgacaggta
     3301 aagagttaga aattattgaa gcaagtgatg cagcgtttgt cgaaaatcta aaagaagccg
     3361 gattccctca agaagcagct gacatgtttt tagcgatcca acatgatatt aaaaaggatc
     3421 agttagctat ttcctctgat gatttagaaa aagtattggg aaaaccatta acttctcttg
     3481 aagcagcatt aaaagaaata ttggatcagg aataatgtat aaagaggttg tgaaaaagcc
     3541 gattagtttt gagtatgaag aaaaattttg tgaaaaagct gttctgcatc aagtaataag
     3601 aagagtcaaa taaaaaatag ctcttcatat gtttcgttta gctggactta ttaccgcgga
     3661 gttggcagga gaacgccgtt tttattcgga aaaaggagct gagacaagat ttttgtctca
     3721 gctcctgttt gtttatcgat aaaaaagtct ggggaagacg atggctcagg ctttgttgga
     3781 aacagagccg attttatcat ttgcttttac atcacgtgcg ggttcgattt ctaagctttg
     3841 gactttgtcg ccatttgtga atacaacgac catatccgtg tttttgcctg cagattgaat
     3901 agcggcaaga tcaactaaag caatcaattg acctcttgcg actttttgac cttcttctac
     3961 atataaagtg aatggttcgc cttttaagtc aacagtgttg atgcccatat gaagcaagac
     4021 ttcgatacca gcatctgttt ggattccgac agcatgtttc gttgggaaaa tgtttgtgat
     4081 cgtaccagcg attggagaga agatttctcc attttctggt aatacagcat agccgtcacc
     4141 catcattttt tccgcgaata catcatctga aacttcagtg attggaatca gtttcccatt
     4201 tgcgatagcg taaatatctt gttcgatcgt ttcaccggca aatgtttctg ttttaaccac
     4261 ggtgtttgtt tgtgcaggtc gtgctgcagc accaccatgt agttttgcca tctcatctgc
     4321 aacgaattgt acttctgtac ctacgatgac ttgcgcattt tttccgtcga tgatcttcat
     4381 accaggtacg ccagtggctt tgattttatt ttgatcgatc aggcctgtat ctttgacttg
     4441 caaacgaagg cgtgtagtac agttatcgat cgaagttaca ttgtcggctc cgcctaatgc
     4501 agcgtagatt ttgcttgctt gtgccgcata tttattgtct gaattagtcg ttgcagtatc
     4561 gtcatctgaa agatctgctt cctcgcggcc aggagtcatc agatggaatt ttttgatagc
     4621 aaagttaaag ccaaagtagt aaatcactgc tgttactaat ccttggacga tcaacatcaa
     4681 tggctgattc gcaatcggat ttttcaaaga taggaagtaa tcgacaaatc cggcactaaa
     4741 ggcaaatcct gctgtccaat ggaagaatgc actcactgct aaagaaatcc cagtgaaaat
     4801 tgcatgcaag acatataatg gccaagcaac gaacataaat gaaaattcaa gtggttctgt
     4861 cacaccagta aagaatgaag caaaagctgc tgctagcatc aaggaagctg tttttgcttt
     4921 gttttctgga cgtgcatttc tgtaaatagc atatgctcct gctggaagcc cgaacatcat
     4981 gattgggaaa aatccagctt gatacattcc tgttacgcct tttgttcctt cagaagccaa
     5041 aaatttacca atatcattga ttcctgctac gtcaaaccag aacacggagt tcattgcttg
     5101 atgcaatcct gtagggatca gtaaacggtt gaagaagcca taaagacctg caccacccca
     5161 gcctaaactc aaaatgaatt tagcaaaaga aactaaagca gagtaaaccg gaggccatac
     5221 gagcagtagc actgcactga tcagcaacat gaaaaggcag ccaagattgg cactagacgc
     5281 ttgccgctaa agaaagataa agccatcggt aattttgctt cgtggaaacg attataaaga
     5341 gctgccgcaa tcaatccggc aataatacca ataaatacgt tactgatctg agcaaaagat
     5401 ggatcaactg attctggttt gatattcaat aaagtagcta ctgaatcagt ttttaaaaca
     5461 tttactggaa cttcaaatgc gaccaaacct gccaaggcag cagcacctga tttgtccact
     5521 gacatcccta gtgctaaccc aacggcaaac agcaaaggta attgacctaa aactgcgtta
     5581 ccgccagctt gtaaaaaatg agcgacaatg tcgttactat aagatgccca ccagtttccg
     5641 atccctacta gtaaagcagc agcaggtaag accgcaactg gaagttgaag ggaacgtccc
     5701 attcgttgta gatatgcttt caattttttt cctcctcatg attgttaaca tttgtttgta
     5761 cgattgcgct tcttaaacgc tcgatatgga tggccaagta acccagttct tctttcgtga
     5821 tcgcgagctg gtattctttg gtcagcaaca cacggatttt tttagaaatc ttatagcttt
     5881 ctgggtattt tcgtttgacc atttctacga tttccgtgtc taaaacagca tattgttgcg
     5941 catgggttcg ttgtagaagt agtcgtaaat gattcaccag tcttgaataa ttcagtgaaa
     6001 atggttctga atggatatct gtttccaatt ctgattccac taaatggatg atgtcagata
     6061 caatcgtcac ttcacggata ctgcgatggt tattgttccg accgcttcgt ccgctgtgaa
     6121 tatggattgc gatatatcct gcttcgtcat aagtatatgg aatcccaagc tcttttgtca
     6181 gatactctac tgcccattga gcaatactga attcatcact gtaaagcact tcgatttctc
     6241 ttaaaagttt gttgcggatg acgataccgt tattaatgtt ctccgcagaa aaagcaatgt
     6301 ggtcggtcaa agcgatcaat acatgttcat tcaacttttc catcaaaaca gtttccgcat
     6361 gatcaatgat cgtttctgct gcaaataaaa agcgttcatc aatctgatta agtaaatttt
     6421 gcaacttgat ttgaccttct ggttccatga caaatatccg ttcgacatca cgatcaaaaa
     6481 tcaaatcatt tctttttctg tcaaatccaa tcccttttcc aatagctact ttttcctgtc
     6541 caccgtcatc taccaatact gcattttgat taaagatctt tttgattttc atgcgcgcac
     6601 cctttttgaa gatttggttt tcacgtttgc tagaccagtt gattgcggcc aaacaaaaat
     6661 agacacgaaa aacctcctag ggaaaccatg ttcctcagag atttttcatg cctagtatga
     6721 tctagtcaca tgaaacgaat tattgttaac gtaatcaatt ttatatagac agttatgttt
     6781 tgtcaattct ttttttataa ttatagtaat ttactggaat tatcagaaat aaaaggaaga
     6841 aataaattaa gaaacttatg ttatttaatc gttggatctt ttgcttatct tattcgttgt
     6901 taaaaagctg aaagcgaagg aaaaaaactc tacttaactg tatttctccg ttaggacatc
     6961 taaaaattta cgagcagcag gagaaagagg ctggtctttt ttccaaatga gattcagtga
     7021 agcttgtaat ggcggatcga gtgggatgaa tattttttcc gtcccataag tattgatgat
     7081 cccatctaga gctaatgcac caaaagtccc gttatcgacc atcaaagaag cattgtacaa
     7141 taagttatat gttccgacga tcgataactg atctatgctg aaacctagcc attcagctag
     7201 ttgactgtcc actgacgttt gccgggaaat gatcaaagga acagttttca gttcctgcgg
     7261 atggatgacg gtttgctttg ctaaaggatg atttcgatgt atcaaaagtc cccaacgatc
     7321 ttgggctggc agtcgaaatg actggtattt ttgtttgttt gtcggttcga ttacgagtcc
     7381 aaaatcaagt aatccgctat ccagtttgtc tatgatatca tctgcattcc cgctatataa
     7441 atgaaactgg atatcaggat gcgccttgat caacgaatga aggctatcag aaatgaaaga
     7501 gattgcttta gtttcgcctc cgccaatgta gatttctcca gagatgattg cgtcatttgt
     7561 taaattgttc gccgtttttt ctaccaacga caagatttct tttccttttg aataaagata
     7621 tcgaccagaa tccgtcaact gtatctctcg ggagccccga gtgaaaagtg tcacaccaag
     7681 ttcttcttct aaatctttca gttgttttga gagagtaggt tgcgtgatat gcagagattt
     7741 tgctgctcgg ctgatatttt tttctctagc tactgctaaa aaatacgtca gtatacgaat
     7801 atccattttt tccctcctgt ttttaaaaaa gcataccact aagtatagtt aaaaggaata
     7861 gttaagtatg ttttataagt attagacatg ataagccgtc cgttataaag taaggataaa
     7921 aggagggagc aaaatgacaa gcctcatcga aaaacaaatc gtcaataaag agattcgagt
     7981 aacggatccg ctctttgaag tgatccatca gatccaagct gaaaacgaag agcctttagc
     8041 agcgctgaat actgggtacc atgaaccagc agatatccgc aaacggctag aaaatatcat
     8101 tagtgacaaa gtagatgata cggtgactgt tctgctgcct ttttatacag atttcggcaa
     8161 acatattagt atagggaaaa atgtctttat caatcgtcaa gtgatgttcg ttgacttagg
     8221 tggcatctgt ttagaagatt cagtattgat tggtcctcgt gtcaacctca tcacggtgaa
     8281 ccatctaacc gatccaaaag aacgaagagg cttgtcagta aagccaatcc acatcaagaa
     8341 aaatgcttgg atcggagcag gagcaactat tctgccaggc gtaacgattg gcgagaatgc
     8401 cattgttgca gcgaatgcaa ctgttacgaa agatgtccca gacaatacca tcgttgcagg
     8461 aatccctgcc aaaatcgtca aacctgtcga acggattatt tgaacagtaa gatgaaataa
     8521 gagatagtga gtataagcaa gagatttttc aaaacaggag gagaatagaa tgaatgattt
     8581 atcagaaaaa gtgattgtca tcatgggcgc ttcaagcgga atcggtgaag caacaacgaa
     8641 gctattagca gaaaaagggg ctaaactagt tatagctgca cgccgagaag atcgcctaaa
     8701 agcaataaaa gaatctttac cagaagctga gctatatatt caaacagcag atgtaagaga
     8761 ttttgcacaa gtccaagcag ttatcgattt agcgatggaa aagttcggtc gaatcgatgt
     8821 attgtacaat aatgcaggaa tcatgccaac cgctccatta gttgaaggtc accgtgatga
     8881 atggcaaaat atgcttgata tcaacatcat gggtgtgtta aatggaattt ccgccgtact
     8941 tccaatcatg gaaaaacaaa aatctgggca tatcatttca actgattctg tggcgggaca
     9001 tgttgtttat cctgattccg ccgtttattg cggaacaaaa tttgctgtcc gtgcaatcat
     9061 ggaaggatta cgtcaagagc aacggcaaaa taacatcaaa tctacgatca tctctccagg
     9121 agctgttcaa actgaattat atcaaaccat cagcgacaaa aaggctgctc ttgaacttca
     9181 tgaagcgcaa aaagaatggg gattgacctc cgaagatata gcttctgctg tagcatttgc
     9241 gatagaaacg ccagaccgga tgtctgtcag taacatgatt atccgtccga cgacacagga
     9301 agtataatcc ataacggata atatccaaag agaattactt gttaaatagt tattcaaacg
     9361 accatcaatt taaggcgtga atggagaaga atattcttca tttacgtctt tttatgtaga
     9421 aaaagcaaat ttatcaaaaa gaaacaaatg ttatttaaat atggacattg acttgctttt
     9481 acttttttag ttacactgtt atttaggaaa agtttcataa ttcaaaggag aaaaaagatg
     9541 aaagcaaaga atttattatt tagcgttatt cttttaggaa atctagcagt tgccaacaca
     9601 acagtgctag ctgaagaatc gacagaaact gtcgcaactc agacatcgag ttcttcagaa
     9661 acaggaattg aaaacgatac agagagtact ttgcagtcag ctactgtctc agaaaataca
     9721 atttcaaccg aagaaccaac aactgaaaca acagaacaaa cacaaaccga agacagtaca
     9781 tcagcagaag ctcagaacaa tccagagaat gcagaagaaa tcacgaacca attgacagat
     9841 gggcaaacat tgacacatac ccatcaagca gatttgtctg aattcgaaaa ctatactggc
     9901 tatgcttttt atgctcgctc atctgctaat tctcaacaag cgttcattga tagtattgcc
     9961 tcaactgcac aaagtttggc aagtgccaat gacttatatg catcagtcat gattgctcaa
    10021 gccatcgttg aaagtggctg ggggaacagt actttggctt ctgcccctaa ttacaatcta
    10081 tttgggatta aaggaagcta taatggccaa tctgttacga tgcctacttc ggaatatgtg
    10141 aatgggcaat ggattacggt gaatgccgct tttagaaaat atccttcgta caaagaatca
    10201 ttgcaagata acgtaacggt attgaagacg acttcttttc agccaggcgt ttactactat
    10261 tccggtgcat ggaaaagcaa tacgaactct tacaaagatg ctacggcttg gctaacaggt
    10321 cgttatgcca ctgcgcctaa ttatggttct acattgaaca atgtaatcga aacctataat
    10381 ctgacacagt atgatacagc accaacttca acttctccga tgtatcgtct gtataaccgt
    10441 catacaggag aacatttata tacattgaat gccggagaaa aaaattatct gccaactgta
    10501 ggctgggaat atgaagggat tgcttggcaa gcaccaaaca gcggacaacc tgtctaccgt
    10561 ttatacaatc cttacagtgg agaccatcat tacacaatgg cacaatctga gatcaactct
    10621 ttaacaaaaa tcggttggcg ttatgaaggt ctaagttttt attctggtgg ttccaagcca
    10681 atttatcgac tatttaatcc aaatgaaaaa actggtaccc atcactatac cttgagtgct
    10741 aaggaaagag attggctaac accgatgggc tggagatatg aaggaatagg attttatggt
    10801 tattaaaaag aggtagtgaa aaagctgccc tgcatcaagt agtaagaaca gccaaacaaa
    10861 aacagatttt catgtttttt gtttggttga acttattacc gcagatgcaa gtttttgaac
    10921 accgtttact caaaaaaggt tgtgacaaca tcttgtcata acttttttat tactgtttaa
    10981 gcatactgat ccaatttcta atattcgacg ggattcttgc aaaaggcgat ttcggaaatt
    11041 tttggcggtg taaaattttc tactgcagaa gagctatctt ctatagtttg ataagaatca
    11101 ataatcttgg ttttagcgtt gtgctttctt tatcattttt tccgttcatt ctacaaaaaa
    11161 tttcttttct ctatactttt ctattgagag cgtttttttt attacacagc tgattctttg
    11221 acttttttct ttttttattc tattattgac aagtcaatcg ttgacgagtc aattattaga
    11281 aaggaggaaa ttatgtcttt agaagagatt agtcaccttt tatatcaaat caaattagcc
    11341 gatcaaaaaa tcgtccagat ctttgaagaa aatatcggta taagtcttac tagatacgag
    11401 atcatgatga gactgaaaga agaaaatcca ttgctacaaa gcagtttgca agaaaagttg
    11461 cagattgatc aaggagcagt tactcgtcat ctgaaggtat tggaagcaaa tggctatgtt
    11521 tcgcgtcaga gaaatcctca gaacaaccgg gaagtgtatg tagcgctaac agaaaaagcc
    11581 cagaagaaaa tcgagcattg tgaaatggac cataaagata caggttccgt gctatccccg
    11641 gctttcagcg atgtagaaat gaaacaattt ctaacacttt tagataaatt gaatacccaa
    11701 ctttgcaata taacaacaga aacgaaagag gttaataata atggaacaaa aaaatgattt
    11761 ttcaacaatt atgactggta gaagatccgt tcgcgtatat gatgaaacat acaaaatccc
    11821 acacgaagaa atgctggaaa tgatcaaaga agcgacgact gcaccttctt ctgtcaatat
    11881 gcaaccatgg cgctttgtgg tcgctgaaag cgaggaagca aaagaaatct tgcgtccttt
    11941 gatccgtttc aatacgagac aaaatgatac ttcttctgca atggtcatga tttttggcga
    12001 tatgcaatgt tacgaatatg gcgaagaaat ctataaccaa gcttacgaat cagggaagat
    12061 gccaaaagaa gtcagagatc aacaattagc tgcgatcatt ccttattaca agagtttttc
    12121 aagagaagaa atgaacgatg tagtcaaagt cgattctagc ttagcggcta tgcaattcat
    12181 gttagttgcg cgtgatcatg gctatgaaac aaatccaatt ggtggattcg aagcagatca
    12241 gttggcagag gcatttggtt tggataaaga ccgttatgtt ccagttatta ttttatcaat
    12301 aggaaaagcg gtagaagaag gttacgagtc tgttcgttta gcaccagaaa agatcactac
    12361 gttcgaataa aaggttttca gttactattc agtagctatt gctttttttg aatcgtgtgt
    12421 ggtaacctaa taaaaaagga ggtcggaaaa atgactgcat cgatgaaatt acgtttcgaa
    12481 cttttgaata acaactaaat aaattcaaaa gacatgtata aacaacaatt tcatggatgg
    12541 agtatgtcca ttttttcgaa cgacagtaga ataggtgaaa gctatgacag atttggtgat
    12601 ttagtcatag ttatttcgtt cagagtagga aaagatgtga tgcagttttt ttgcaaacag
    12661 atttttctgc tcttttttag tatcgataat tgttgtttta catgaaatag gagtgtaaaa
    12721 catatggaaa atttagcagt aaatataaca aatctacaag tcagctttgg caaccagcta
    12781 gaactatcta ttgattctct tcgtgtctat cagcaagatc gtatagggat cataggagaa
    12841 aatggtgtcg gtaaatcgac tttgctcaaa ctaatagccg gtgaactttt tcccgatcat
    12901 gggaaaatcc aaacagagat caccttcaac tacctgcctc aattaaccta tctcgctgaa
    12961 gcaaaggacc taaatttgga attagccagt catttccagt taagactgga agaaacttca
    13021 gagcggaaat ggagcggagg ggaagaacga aagatcgagt tgatacgtct tctttcttct
    13081 tatgaacaag ggatgcttct agacgagcca acaacccatc tagatagaaa aagtattgat
    13141 cgactgattg aagagcttcg ttattattat ggtacgctgg tttttgttag tcatgatcgc
    13201 tattttctag atgagctagc atcgaaaatc tgggaagtaa aagacggaga aatccgagag
    13261 ttttcgggga attatagtgc ctatctcact caaaaggaat tggagaaaaa gactcagcta
    13321 cgagaagcag agtcgatcat gaaagagaaa aaacgattgg aaaaatcgat ccaagaaaag
    13381 aaaaaacaag cggaaaagtt ggaaaaagtg tccagtaaaa agaaaaagca acaaatcaga
    13441 ccggatcggt tgtcttcctc taaacaaaaa gacagtgtac aaaaagccat ccaaaagaat
    13501 gcgaaaacat tagagagaag actccaaaaa ataggagaaa caaccaagcc gcagcagatg
    13561 aaacaaatcc gttttccagt accaaaatct cttgaactcc acagccgcta tcccatcatg
    13621 ggacaaaatg tccaattgga aagaagtggg agaacattac tggtaaatgg tgattttcag
    13681 ttttctttag gtaaaaaaat cgcgattgtc ggtgaaaatg gttcaggtaa gacaacctta
    13741 ttagaacata tccgcaaaca aggagaagga atccttctct ctccgaaagt aagctttcaa
    13801 gtatatcagc aaaagggtta tcaaatgaca tctgaagaat ccatcattcg ttttgtcatg
    13861 agacaaacag agttttcaga atcgcttgtc cgtagtttgc tgaatcactt agggtttgct
    13921 caggaaactt tgacgaaacc gttatgtaca ttaagtgggg gagaagcgac ccgtctgacg
    13981 attgctttgc tttttactaa gccaagtaat gtgttgctgt tagatgaacc gactaatttt
    14041 attgatatgg caacgatcga agctttagag aagctaatgc aaatatatcc gggaacgatt
    14101 ttgtttactt ctcatgattc ttactttgtc gagcgtacgg ctgatgaagt ttatgaaata
    14161 aaagggcaga aaataaaaaa agtacttacg agaaattttt aaatgtgcta cgatgatttt
    14221 cgtgacaaat ttaaaggagt ggtacagatt gcctacaaat aaaaaagaag gaataatttt
    14281 tacaacaatg atgtgttttt tgatggtctt ggggatgagc gcgtataatt tatggctgca
    14341 tcaagatctt catttatcaa gtttgttgat tggtctagtg cctggattcg tggtggcttt
    14401 cattttggat gtcttcatca tcggtgtgct ggcgaaaaaa attgccttta aactaccagt
    14461 aaatccagaa agcaaggtcc aaatgatctt aactatttca tgcttgatgg ttttaggaat
    14521 ggtgacatgt atgtctttat tcggtgtgat catggaacaa ggaatacctg atcaattatt
    14581 ctctgcttat atgaatgctt ggaaaatgaa tgtgattgcc gctttaccgt tgcagttgat
    14641 catcgttggc ccgctttcaa gaagtgtgct aaaagcaatt caaagtaatg aatgatggaa
    14701 cactgttatt tgaaaaaagg ccttggcatg ctttttgtca tagtctcttg aatgcagttc
    14761 tagaaaaata gtgatccgaa cgataggttt tggcctgtcg ttcggatcgc tttttctttt
    14821 ccaactgtat tgctctttta ttgaagataa tttgctcttc aatagatatt tctagaaagg
    14881 ttctgtttat cccttactca aaaaactgct gcatttgttc tcttacttgc tgacaccatt
    14941 ggagttcctg ctgacaaagc ataattctgc gctggatcac aacataacgt ccaaattctt
    15001 gtttcttgtc tagcgagagg tcattcaatt ttttttgatt tttttgcatg attttgttca
    15061 gctgttgttc tctagtgaat agcagtccag tagctgttgt tttatcgagc gtcgaaaaag
    15121 catagatttt tgctagaaat tcatcttttt gcgtaggagc gggagtttcc tctttgatcc
    15181 attcctctac aagagcacgt ccttcatcag tcaagtggta gatctttttt tgagagtgct
    15241 cgtctttgtc caaaacggtc acataaccct gctggtctaa cttggcaagc gttgtataga
    15301 tctgactgtg atgggcttcc caaaaaattg tgatcagctg cttcaattca tacccagaac
    15361 gtggtttgga taaaagcacg cataataata tataggctaa agtattcata aaaatcctac
    15421 ctctcgtctt attttcttta ttatatcaca gtaaagtttc cacgttttac atgtgcatat
    15481 ttacatgtaa aataaatcat gtatgatgct aatagagaga aagggatgga aatattggag
    15541 aatcaaatag atatacaaga acaaacaaat gaagaaacaa aggggaaaag ttcagcgcta
    15601 gttgtagcag ctacctgtat gatcgctttt atgggagtgg gtctagtaga cccaatctta
    15661 aaaacgattg ctgcacagtt aaacgctacg ccagctgaaa cgaccctgtt atttactagt
    15721 tacatgttag tcactggtgt ggtgatgcta tttacaggtt tcttatctag tcggatcggt
    15781 ttgaaaaaaa cattgatgac aggattgttt atcatcgttg tattcgctgg tcttggcgga
    15841 ttatctggga atatcggaat gctgattggg ttacgtgccg gctggggtgt cgggaatgct
    15901 ttatttgtct ctactgcttt agcaacgatc gtcagtattt tagttggtaa cacagaaaaa
    15961 gcaatcatga tgtatgaagc agcacttggc tgcggaatgg ctgttggccc tctagtcggt
    16021 ggggtcttgg gcagtggttc atggcgttat ccattttttg gggtagcatt tttgatgttt
    16081 tgcggtttta tagccctagc ggtactttta cctgaaactg agaaaccaaa gagaaaagta
    16141 ggcctttggg aaggtgtcaa agcgttaagc aatcataaac ttcgttcagt tggactaatc
    16201 gcattgttat ataatttcgg attctttact ttacttgcgt atgcgccatt tttattgatt
    16261 ggctattctg aattagaagt cggttttgta ttttttggct ggggtgtgct tctggctatt
    16321 gcttcgattt tcgttgcacc ggctcttgag cggaagttta cgacttatcg gacaatttta
    16381 gcagcatttg tcttattcat tatttgtttg ctgttattag gagctggaac ttctatgcca
    16441 gtccttgtac cgatcagcat tgtgttagca ggatttttcc aaggaattat caatacactg
    16501 ttgacgacga ttgctatgga aataccaggt ctgcaacgaa acgttgcttc gtccagctat
    16561 agttttgtcc gctttttcgg tggagcttta gcaccattta ttgcaggtgt gattggtgaa
    16621 catattggtg aaagctatgc gttttatttt gcaggtgcca ttgttcttat cagtgtatgt
    16681 tgtatcattt atcaccgtaa ttattttgct acaaatgaag aagaataaga aatcagaggt
    16741 tgtgaaaaag ctgtcctgca tcaagtcata agaa
//
LOCUS       DASHRQ010000210         8062 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.guided.097, whole genome shotgun sequence.
ACCESSION   DASHRQ010000210 DASHRQ010000000
VERSION     DASHRQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.guided.097"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            128..1564
                     /locus_tag="U2M88_003020"
     CDS             128..1564
                     /locus_tag="U2M88_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEM8375312.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="U2M88_003021"
     CDS             1753..4200
                     /locus_tag="U2M88_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEM8375313.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="U2M88_003022"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="U2M88_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEM8375314.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="U2M88_003023"
     CDS             complement(5690..6793)
                     /locus_tag="U2M88_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEM8375315.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="U2M88_003024"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="U2M88_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEM8375316.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2697 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga aa
//
LOCUS       DASHRQ010000211         6992 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.guided.105, whole genome shotgun sequence.
ACCESSION   DASHRQ010000211 DASHRQ010000000
VERSION     DASHRQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.guided.105"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            109..192
                     /locus_tag="U2M88_003025"
     CDS             109..192
                     /locus_tag="U2M88_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEM8375317.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            317..1054
                     /gene="erm(B)"
                     /locus_tag="U2M88_003026"
     CDS             317..1054
                     /gene="erm(B)"
                     /locus_tag="U2M88_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEM8375318.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1224..1484
                     /locus_tag="U2M88_003027"
                     /pseudo
     CDS             1224..1484
                     /locus_tag="U2M88_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1647..2312
                     /locus_tag="U2M88_003028"
                     /pseudo
     CDS             1647..2312
                     /locus_tag="U2M88_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2348..2593
                     /locus_tag="U2M88_003029"
     CDS             2348..2593
                     /locus_tag="U2M88_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEM8375319.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2696..3565
                     /locus_tag="U2M88_003030"
     CDS             2696..3565
                     /locus_tag="U2M88_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEM8375320.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3546..4280
                     /locus_tag="U2M88_003031"
     CDS             3546..4280
                     /locus_tag="U2M88_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM8375321.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4313..5225
                     /gene="ant(6)"
                     /locus_tag="U2M88_003032"
                     /pseudo
     CDS             4313..5225
                     /gene="ant(6)"
                     /locus_tag="U2M88_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5222..5764
                     /gene="sat4"
                     /locus_tag="U2M88_003033"
     CDS             5222..5764
                     /gene="sat4"
                     /locus_tag="U2M88_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HEM8375322.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5857..6651
                     /locus_tag="U2M88_003034"
                     /note="aph(3')-IIIa"
     CDS             5857..6651
                     /locus_tag="U2M88_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEM8375323.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT         2449 a         1048 c         1458 g         2037 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccatt ttgtgtataa taggaattga
       61 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      121 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
      181 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      241 cagataacta aaattacaaa caaatcgttt aacttctgta tttatttaca gatgtaataa
      301 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      361 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      421 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      481 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaacat
      541 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      601 taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      661 tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      721 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      781 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      841 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      901 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      961 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
     1021 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg
     1081 gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt
     1141 ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa
     1201 ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgtggc aacaatattg
     1261 tattaaaaaa atcgttttat ggttgttcaa attatcctga atgtaagttt actttagctg
     1321 aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag
     1381 aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa
     1441 ttggggaaaa gggatatatt gattttatct ctttttcaaa ataaacatgg ggctgaccca
     1501 aaagtagctc ctatatgaaa aatagaggtc tttccattta gatagtagtg gaaagacctc
     1561 tatttatctt ttgaaagtga atacaaaaat aaagcacact ctgctataat ataggtgatt
     1621 aatccatttg agagaggtgc tttatcatgt atcaaaatta taccataaac caactttgtt
     1681 tgcctataga tcttgaaatt aaattagaag aaaatgattt tgctcatgcc attgtgcagt
     1741 ttgttgactc tattcccgat gaagtctttc ttccttatta tcaatctatg ggtagacccc
     1801 aatatcatcc tcgtatgcta ctttccatta ttctttgtgc ctatattcaa ggggtttatt
     1861 cgggccgcca aatacaaaat atgctgatag atagtattcg gatgcgctac ttatctcaag
     1921 agcaattccc taactttcgt actattaatc gttttcgcgt tcatccgatt atgaacgata
     1981 ttctagatca ttcatttgtt caatttcgtg agctactggt tcaaagtggc ttaatcagtg
     2041 gcagtgctct atacattgat ggtacaaaaa ttgaagcgga tgcgaataaa tactctttcg
     2101 tttggaaaaa aagtatttta aagtataagg atagccttga tcaaaaagcc cttgaaaact
     2161 accaacaaat ggtggaaaca aaaatcttac ctgagttaat agatgattta aaggatgaac
     2221 tttctataga agagattgaa aggattcgtc aatctttgga agaaaaggaa aatcaattaa
     2281 ttgaagcgat tgaccaaacc gaaacagtag aagggggctg acccattatt aataaggagt
     2341 ggacaacatg attgatgtaa atgagctatt ggaagaagct attagagaaa ctgaaaatct
     2401 aaatgatggc gaagtttttc ttgttaaaga tttattcaag ggatatgtgg ggaataggat
     2461 acccagaaag gatcgacttc tgcttggaac gttattttta aaccatgtta gcaaaatgaa
     2521 tggtaattta aaggctattg aaaagacttc atctaatcag cagaggtata aaaagacaat
     2581 tgataagttt taactatctt ttgtgaaatt aaaaaaatta taaatatgtt gagtagaagc
     2641 aaaagatttg gcttggaatt tttacagcag tgaatcgata aaaggagcat gcttgatggt
     2701 agataatatt attaaatcag tagcagagaa attatcctct ctgtcttata tagaaggtat
     2761 tgttttaggt ggttcacgtg caaggggcac ccatacagag gattcggata tagatatcgg
     2821 catctattac aattcagaat catttgacat aaatactatt aatcaattcg ctacaaagct
     2881 ggatgatgag catagaaata accttgttgt acctcccgga gcatggggtg attggattaa
     2941 tggcggcgga tggttagtca taaacgggta tcatgtggat ttaattttac gtgatattaa
     3001 acgtgtggaa caaataatga aagatacaga gcacggaatt gttactgcca attatcagac
     3061 tgggcatccc catggttata ttagtgcaat gtatcgagga gaattagcga ttagcaaaat
     3121 actatatgct aagaatgaaa gcttatgcga attaaaaaaa caggcagaaa cttatcccaa
     3181 tgctttgcag aaaagtttag ttaacttttt tatgtttgaa gcagggttct ctttaatgtt
     3241 tgtaaaagca aattcgggaa cagacgataa atattatatt gcgggtcatg tttttcgtat
     3301 agtttcatgt ttaaatcaag tgttatttgc atgtaataat gcttattgta tcaacgaaaa
     3361 gaaagctata aaactgcttg aaacttttga acataaacct gaaaaatata ccgagaaggt
     3421 aaatcatatt tttgaagtac tcggtatctc actttttgaa tgctacgaca tgaccgagaa
     3481 gctttataat gaagtgaatg aaattgtatc ggagataaat aactttttaa acgaggagag
     3541 ttcagatgaa agaaaacaaa tatgatgata atatattttt tcaaaaatac agtcaaatga
     3601 gtcgctcgca gaaaggactg gctggtgcgg gagaatggga gactttgaaa aagatgctac
     3661 ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg cactgtatat
     3721 atgcgatgga aaacggtgct tcctctgtag taggtgttga tatttctcat aaaatgctcg
     3781 aagtagcaaa aggaaaaacc cattttccac agattgaata tgaatgctgt gccatagaag
     3841 atgtggattt cccagaggag agctttgatg taatactaag ttcgcttgcg tttcattatg
     3901 tagcagacta tgagaattta ataaaaaaga tatataggat gctgaaggct ggtggcaatt
     3961 tagtttttac agttgaacat cctgttttta ctgctcatgg aacacaagac tggtattata
     4021 acgaaaaagg agaaatactg catttcccgg tggacaatta ttattatgag ggcaaacgga
     4081 cagctatgtt tttggaagaa aaggttacaa aatatcatag aacactgacc acatatctaa
     4141 atacactgct ttcaaatagt tttataataa atcagattgt ggagccacag ccgccagaga
     4201 acatgatgga tattccgggg atggcggatg aaatgcgacg cccaatgatg ctgattgtat
     4261 cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg aggagtatgt aaatgagatc
     4321 agaaaaagaa atgatggatt tagtactttc tttagcagaa caggatgaac gaacgtattc
     4381 gaattgtgac ccttgagggg tcacgcgcaa atattaatat acctaaagat gaatttcagg
     4441 attatgatat tacatatttt gtaagtgata tagaaccgtt tatatctaat gatgactggc
     4501 ttaatcaatt tgggaatata ataatgatgc aaaagccgga ggatatggaa ttattcccac
     4561 ctgaagaaaa gggattttcc tatcttatgc tatttgatga ttacaataaa attgatctta
     4621 ccttattgcc cttggaagag ttagataatt acctaaaggg cgataaatta ataaaggttc
     4681 taattgataa agattgtaga attaaaaggg acatagttcc gactgatata gattatcatg
     4741 taagaaagcc aagcgcaagg gagtatgatg attgctgcaa tgaattttgg aatgtaacac
     4801 cttatgttat taaaggattg tgccgtaagg aaattttatt tgctattgat cattttaatc
     4861 agattgttcg ccatgagctg ctgagaatga tatcatggaa ggtcggcatc gaaacaggct
     4921 ttaaattaag tgtaggcaag aactataagt ttattgaaag gtatatatcc gaggatttgt
     4981 gggagaaact tttgtccacc taccggatgg attcctatga aaacatatgg gaagcattat
     5041 ttctatgcca tcaattgttc agggcggtat ccggtgaggt ggcggaaagg cttcattatg
     5101 cctatccgga gtatgatagg aatataacaa aatataccag ggacatgtat aaaaaataca
     5161 ctggtaaaac cggctgcctg gatagcacat atgccgctga tatagaagag aggcgggaac
     5221 agtgattaca gaaatgaaag cagggcacct gaaagatatc gataaaccca gcgaaccatt
     5281 tgaggtgata ggtaagatta taccgaggta tgaaaacgag aattggacct ttacagaatt
     5341 actctatgaa gcgccatatt taaaaagcta ccaagacgaa gaggatgaag aggatgagga
     5401 ggcagattgc cttgaatata ttgacaatac tgataagata atatatcttt actaccaaga
     5461 cgataaatgc gtcggaaaag ttaaactgcg aaaaaattgg aaccggtacg cttatataga
     5521 agatatcgcc gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc ttatcaatat
     5581 atctatagaa tgggcaaagc ataaaaactt gcatggacta atgcttgaaa cccaggacaa
     5641 taaccttata gcttgtaaat tctatcataa ttgtggtttc aaaatcggct ccgtcgatac
     5701 tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt atttaaggtt
     5761 ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt cttggggtat
     5821 ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag aatatcaccg
     5881 gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct
     5941 gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc
     6001 cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa
     6061 ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg
     6121 ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct
     6181 gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg
     6241 gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat
     6301 aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt taaagatccg
     6361 cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac
     6421 ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat
     6481 cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc
     6541 agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag
     6601 cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta gtacctagat
     6661 ttagatgtct aaaaagcttt aactacaagc tttttagaca tctaatcttt tctgaagtac
     6721 atccgcaact gtccatactc tgatgtttta tatcttttct aaaagttcgc tagatagagt
     6781 tctatattag agatgtaaag agttttggtg aagaggtttg tgagtatggc ttttataacc
     6841 agggtgcaat gagaagcact aacagcagct agcttaagaa cgcataggat atacttttta
     6901 tggaagtcac agaggaggaa taaaatttga acaggattaa cagagtaacc tctattggtt
     6961 ctgttgcaaa gttttaaata aagaataaaa tc
//
LOCUS       DASHRQ010000212         6376 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.guided.108, whole genome shotgun sequence.
ACCESSION   DASHRQ010000212 DASHRQ010000000
VERSION     DASHRQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.guided.108"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="U2M88_003035"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="U2M88_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEM8375324.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="U2M88_003036"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="U2M88_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HEM8375325.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            3976..5238
                     /locus_tag="U2M88_003037"
     CDS             3976..5238
                     /locus_tag="U2M88_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEM8375326.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            5483..6370
                     /locus_tag="U2M88_003038"
     CDS             5483..6370
                     /locus_tag="U2M88_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HEM8375327.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKKWFCCKVLNKE"
BASE COUNT         2211 a          906 c         1312 g         1947 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt tacatatgag ttatgcagtt tgtagaatgc aaaaagtgaa atcagctgga
     4021 ctaaaaggca tgcaatttca taatcaaaga gagcgaaaaa gtagaacgaa tgatgatatt
     4081 gaccatgagc gaacacgtga aaattatgat ttgaaaaatg ataaaaatat tgattacaac
     4141 gaacgtgtca aagaaattat tgaatcacaa aaaacaggta caagaaaaac gaggaaagat
     4201 gctgttcttg taaatgagtt gctagtaaca tctgaccgag atttttttga gcaactggat
     4261 ccaggagaac aaaaacgatt ttttgaggaa agttataaat tattttccga acgatatggc
     4321 aagcaaaata ttgcttatgc aacagttcat aatgatgagc aaacccctca catgcattta
     4381 ggtgttgtgc ctatgcgtga tggaaaactg caaggaaaaa atgtgtttaa tcgtcaagaa
     4441 ctgttatggc tacaagataa attccccgag catatgaaaa aacagggttt tgagttgaag
     4501 cgtggtgaac gtggctctga ccgtaaacat attgagacag ctaaatttaa aaaacaaact
     4561 ttggaaaaag agattgattt tctagaaaaa aatttagcag ttaaaaaaga tgaatggact
     4621 gcttatagcg ataaagttaa atcagattta gaagtaccag cgaaacgaca catgaaaagt
     4681 gttgaagtgc caacgggtga aaagtccatg tttggtttgg gaaaagaaat aatgaaaaca
     4741 gaaaagaaac caaccaaaaa tgttgttata tcggagcgtg attataaaaa cttagtgact
     4801 gctgcgagag ataacgatag gttaaaacag catgttagaa atctcatgag tactgatatg
     4861 gcgagagaat ataaaaaatt aagtaaagaa catgggcaag ttaaagaaaa atatagtggt
     4921 cttgtagagc gatttaatga aaatgtaaat gattataatg agttgcttga agaaaacaag
     4981 tctttaaagt ctaaaataag cgatttaaag cgtgatgtga gtttaatcta tgaaagcact
     5041 aaggaattcc ttaaggaacg tacagacggc ttaaaagcct ttaaaaacgt ttttaagggg
     5101 tttgtagaca aggtaaagga taaaacagca caattccaag aaaaacacga tttagaacct
     5161 aaaaagaacg aatttgaact aactcataac cgagaggtaa aaaaagaacg aagtcgagat
     5221 cagggaatga gtttataaaa taaaaaaagc acctgaaaag gtgtcttttt ttgatggttt
     5281 tgaacttgtt ctttcttatc ttgatatata tagaaataac gtcattttta ttttagttgc
     5341 tgaaaggtgc gttgaagtgt tggtatgtat gtgttttaaa gtattgaaaa cccttaaaat
     5401 tggttgcaca gaaaaacccc atctgttaaa gttataagtg accaaacaaa taactaaata
     5461 gatgggggtt tcttttaata ttatgtgtcc taatagtagc atttattcag atgaaaaatc
     5521 aagggtttta gtggacaaga caaaaagtgg aaaagtgaga ccatggagag aaaagaaaat
     5581 cgctaatgtt gattactttg aacttctgca tattcttgaa tttaaaaagg ctgaaagagt
     5641 aaaagattgt gctgaaatat tagagtataa acaaaatcgt gaaacaggcg aaagaaagtt
     5701 gtatcgagtg tggttttgta aatccaggct ttgtccaatg tgcaactgga ggagagcaat
     5761 gaaacatggc attcagtcac aaaaggttgt tgctgaagtt attaaacaaa agccaacagt
     5821 tcgttggttg tttctcacat taacagttaa aaatgtttat gatggcgaag aattaaataa
     5881 gagtttgtca gatatggctc aaagatttcg ccgaatgatg caatataaaa aaattaataa
     5941 aaatcttgtt ggttttatgc gtgcaacgga agtgacaata aataataaag ataattctta
     6001 taatcagcac atgcatgtat tggtatgtgt ggaaccaact tattttaaga atacagaaaa
     6061 ctacgtgaat caaaaacaat ggattcaatt ttggaaaaag gcaatgaaat tagactatga
     6121 tccaaatgta aaagttcaaa tgattcgacc gaaaaataaa tataaatcgg atatacaatc
     6181 ggcaattgac gaaactgcaa aatatcctgt aaaggatacg gattttatga ccgatgatga
     6241 agaaaagaat ttgaaacgtt tgtctgattt ggaggaaggt ttacaccgta aaaggttaat
     6301 ctcctatggt ggtttgttaa aagaaataca taaaaaatgg ttctgttgca aagttttaaa
     6361 taaagaataa aatccc
//
LOCUS       DASHRQ010000213         6376 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.guided.109, whole genome shotgun sequence.
ACCESSION   DASHRQ010000213 DASHRQ010000000
VERSION     DASHRQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.guided.109"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="U2M88_003039"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="U2M88_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEM8375328.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="U2M88_003040"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="U2M88_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HEM8375329.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            3976..5238
                     /locus_tag="U2M88_003041"
     CDS             3976..5238
                     /locus_tag="U2M88_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEM8375330.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            5483..6370
                     /locus_tag="U2M88_003042"
     CDS             5483..6370
                     /locus_tag="U2M88_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HEM8375331.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKKWFCCKVLNKE"
BASE COUNT         2210 a          906 c         1313 g         1947 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt tacatatgag ttatgcagtt tgtagaatgc aaaaagtgaa atcagctgga
     4021 ctaaaaggca tgcaatttca taatcaaaga gagcgaaaaa gtagaacgaa tgatgatatt
     4081 gaccatgagc gaacacgtga aaattatgat ttgaaaaatg ataaaaatat tgattacaac
     4141 gaacgtgtca aagaaattat tgaatcacaa aaaacaggta caagaaaaac gaggaaagat
     4201 gctgttcttg taaatgagtt gctagtaaca tctgaccgag atttttttga gcaactggat
     4261 ccaggagaac aaaaacgatt ttttgaggaa agttataaat tattttccga acgatatggc
     4321 aagcaaaata ttgcttatgc aacagttcat aatgatgagc aaacccctca catgcattta
     4381 ggtgttgtgc ctatgcgtga tggaaaactg caaggaaaaa atgtgtttaa tcgtcaagaa
     4441 ctgttatggc tacaagataa attccccgag catatgaaaa aacagggttt tgagttgaag
     4501 cgtggtgaac gtggctctga ccgtaaacat attgagacag ctaaatttaa aaaacaaact
     4561 ttggaaaaag agattgattt tctagaaaaa aatttagcag ttaaaaaaga tgaatggact
     4621 gcttatagcg ataaagttaa atcagattta gaagtaccag cgaaacgaca catgaaaagt
     4681 gttgaagtgc caacgggtga aaagtccatg tttggtttgg gaaaagaaat aatgaaaaca
     4741 gaaaagaaac caaccaaaaa tgttgttata tcggagcgtg attataaaaa cttagtgact
     4801 gctgcgagag ataacgatag gttaaaacag catgttagaa atctcatgag tactgatatg
     4861 gcgagagaat ataaaaaatt aagtaaagaa catgggcaag ttaaagaaaa atatagtggt
     4921 cttgtagagc gatttaatga aaatgtaaat gattataatg agttgcttga agaaaacaag
     4981 tctttaaagt ctaaaataag cgatttaaag cgtgatgtga gtttaatcta tgaaagcact
     5041 aaggaattcc ttaaggaacg tacagacggc ttaaaagcct ttaaaaacgt ttttaagggg
     5101 tttgtagaca aggtaaagga taaaacagca caattccaag aaaaacacga tttagaacct
     5161 aaaaagaacg aatttgaact aactcataac cgagaggtaa aaaaagaacg aagtcgagat
     5221 cagggaatga gtttataaaa taaaaaaagc acctgaaaag gtgtcttttt ttgatggttt
     5281 tgaacttgtt ctttcttatc ttgatatata tagaaataac gtcattttta ttttagttgc
     5341 tgaaaggtgc gttgaagtgt tggtatgtat gtgttttaaa gtattgaaaa cccttaaaat
     5401 tggttgcaca gaaaaacccc atctgttaaa gttataagtg accaaacaaa taactaaata
     5461 gatgggggtt tcttttaata ttatgtgtcc taatagtagc atttattcag atgaaaaatc
     5521 aagggtttta gtggacaaga caaaaagtgg aaaagtgaga ccatggagag aaaagaaaat
     5581 cgctaatgtt gattactttg aacttctgca tattcttgaa tttaaaaagg ctgaaagagt
     5641 aaaagattgt gctgaaatat tagagtataa acaaaatcgt gaaacaggcg aaagaaagtt
     5701 gtatcgagtg tggttttgta aatccaggct ttgtccaatg tgcaactgga ggagagcaat
     5761 gaaacatggc attcagtcac aaaaggttgt tgctgaagtt attaaacaaa agccaacagt
     5821 tcgttggttg tttctcacat taacagttaa aaatgtttat gatggcgaag aattaaataa
     5881 gagtttgtca gatatggctc aaagatttcg ccgaatgatg caatataaaa aaattaataa
     5941 aaatcttgtt ggttttatgc gtgcaacgga agtgacaata aataataaag ataattctta
     6001 taatcagcac atgcatgtat tggtatgtgt ggaaccaact tattttaaga atacagaaaa
     6061 ctacgtgaat caaaaacaat ggattcaatt ttggaaaaag gcaatgaaat tagactatga
     6121 tccaaatgta aaagttcaaa tgattcgacc gaaaaataaa tataaatcgg atatacaatc
     6181 ggcaattgac gaaactgcaa aatatcctgt aaaggatacg gattttatga ccgatgatga
     6241 agaaaagaat ttgaaacgtt tgtctgattt ggaggaaggt ttacaccgta aaaggttaat
     6301 ctcctatggt ggtttgttaa aagaaataca taaaaaatgg ttctgttgca aagttttaaa
     6361 taaagaataa aatccc
//
LOCUS       DASHRQ010000214         4995 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.guided.124, whole genome shotgun sequence.
ACCESSION   DASHRQ010000214 DASHRQ010000000
VERSION     DASHRQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.guided.124"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(30..2180)
                     /locus_tag="U2M88_003043"
                     /pseudo
     CDS             complement(30..2180)
                     /locus_tag="U2M88_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2326..2901
                     /locus_tag="U2M88_003044"
     CDS             2326..2901
                     /locus_tag="U2M88_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM8375332.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3115..3810
                     /gene="vanR"
                     /locus_tag="U2M88_003045"
     CDS             3115..3810
                     /gene="vanR"
                     /locus_tag="U2M88_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEM8375333.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3788..4942
                     /locus_tag="U2M88_003046"
     CDS             3788..4942
                     /locus_tag="U2M88_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEM8375334.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1560 a          939 c          967 g         1529 t
ORIGIN      
        1 aattttcttc tttcagcttt ttatttccca cttgtaaatc ggtagatggt ggctcctttt
       61 ccagttccat aatgtggatt tgcatctgca tgtagtgatg aaacacctag ctgcattctc
      121 ataccatctg acgaagatgt tgtaccgtcg ccccaataga aaggcaattg taatttatga
      181 tgaaagttta ctaatatggc ttgggcttta ttcatggcat cttcatacat gcgccattga
      241 gatacattgg ctagttgctt atatgtaagt ccgggtgtgg cttcggccat cttgctcaag
      301 ccaatattca ttcccattcc taaaagggca gccatgataa tgattgtttc ttccttatct
      361 ggttttcgat tattggaagc atgagtgaat tgctcatgaa atcctgttat atgggccaca
      421 tccatgagta aatcagttaa ttttattctt ggtagcatct gataaaggct tgcactaaat
      481 ttttttgctt cttctggaac atctttttct aagcgtgcaa gtgatagctt tcctttttca
      541 agagaaaccc catctaactt attggaattg gcagctaacc actttaacct ttcattaaag
      601 ctgctggttc tctccgttat ataatcttcg aatgataaac taactgataa tctcgtattc
      661 cccttcgatt gattccatgt atcttccgaa aacaaatatt cctcaaaatc cctatattgt
      721 ctgctgccaa caatggaaac atctcctgcc cgaacatgct cccgaagttc tgttaaaaca
      781 gccatttcat agtaatgacg attaattgtt gtaccatcat cctcgtataa atgtcttttc
      841 catcgttttg aaataaaatc cacaggtgag tcatcaggca cttttcgctt tccagattcg
      901 ttcattcctc ggataatctc aacagcttgt aaaagtggct catttgcctt tgtagaatga
      961 aattccaata ctcttaatag cgttggcgta tattttctta gtgaataaaa ccgtttttgc
     1021 agtaagtcta aataatcata gtcggcagga cgtgcaagtt cctgagcctc ttctactgaa
     1081 gagacaaagg tattccattc aataaccgat tctaaaacct taaaaacgtc taatttttcc
     1141 tctcttgctt taattaatgc ttgtccgatg ttcgtaaagt gtataacttt ctcatttagc
     1201 tttttaccgt tttgtttctg gatttcctct tgagccttac gaccttttga taacaaacta
     1261 agtatttgcc tatcatgaat ttcaaacgct ttatccgtta gctcctgagt aagttgtaat
     1321 aaatagatgg ttaatatcga ataacgttta ttttcttgaa agtcacggaa tgcatacggc
     1381 tcgtatcttg agcctaagcg agacagctgc aacaggcggt tacggtgcaa atgactaatt
     1441 tgcactgttt ctaaatccat tcctcgtatg tattcgagtc gttctattat ttttagaaaa
     1501 gtttcgggtg aaggatgacc cggtggctct tttaaccaac ccaatatcgt tttattggat
     1561 tcggatggat gctgcgaggt aataatccct tcaagctttt ctttttgctc atttgttaga
     1621 gatttactaa ccgtattaaa tagcttcttt tcagccattg cccttgcttc ccacaccatt
     1681 ctttcaagtg tagtgatagc aggcagtata attttgtttt ttcttagaaa atctatgcat
     1741 tcatgcagta gatgaatggc atcaccattt tccaaagcta attgatgaag gtacttaaat
     1801 gtcattcgat attcactcag ggtaaaagtt acaaagtcgt attcacttcg aatttctttc
     1861 aaatgatccc aaagtgtatt ttccctttga ggataatgat caagcgagga tggactaaca
     1921 ccaatctgtt tcgatatata ttgtatgacc gaatctggga tgcttttgat atgagtgtat
     1981 ggccaaccgg gataccgaag aacagctaat tgaacagcaa atcctaaacg gttttcttcc
     2041 ctccttcgct tattaactat ttctaaatcc cgtttggaaa aagtgaagta ggtccccagt
     2101 atccattcat cttcagggat ttgcataaaa gcctgtctct gttccggtgt aagcaattct
     2161 ctacctctcg caattttcat tcagtatcat tccatttctg tattttcaat ttattagttc
     2221 aattatatat caatagagtg tactctattg atacaaatgt agtagactga taaaatcata
     2281 gttaagagcg tctcataaga cttgtctcaa aaatgaggtg atattttgcg gaaaatcggt
     2341 tatattcgtg tcagttcgac taaccagaat ccttcaagac aatttcagca gttgaacgag
     2401 atcggaatgg atattatata tgaagagaaa gtttcaggag caacaaagga tcgcgagcaa
     2461 cttcaaaaag tgttagacga tttacaggaa gatgacatca tttatgttac agacttaact
     2521 cgaatcactc gtagtacaca agatctattt gaattaatcg ataacatacg agataaaaag
     2581 gcaagtttaa aatcactaaa agatacatgg cttgatttat cagaagataa tccatacagc
     2641 caattcttaa ttactgtaat ggctggtgtt aaccaattag agcgagatct tattcggatg
     2701 agacaacgtg aagggattga attggctaag aaagaaggaa agtttaaagg tcgattaaag
     2761 aagtatcata aaaatcacgc aggaatgaat tatgcggtaa agctatataa agaaggaaat
     2821 atgactgtaa atcaaatttg tgaaattact aatgtatcta gggcttcatt atacaggaaa
     2881 ttatcagaag tgaataatta gccattctgt attccgctaa tgggcaatat ttttaaagaa
     2941 gaaaaggaaa ctataaaata ttaacagcct cctagcgatg ccgaaaagcc ctttgataaa
     3001 aaaagaatca tcatcttaag aaattcttag tcatttatta tgtaaatgct tataaattcg
     3061 gccctataat ctgataaatt attaagggca aacttatgtg aaagggtgat aactatgagc
     3121 gataaaatac ttattgtgga tgatgaacat gaaattgccg atttggttga attatactta
     3181 aaaaacgaga attatacggt tttcaaatac tataccgcca aagaagcatt ggaatgtata
     3241 gacaagtctg agattgacct tgccatattg gacatcatgc ttcccggcac aagcggcctt
     3301 actatctgtc aaaaaataag ggacaagcac acctatccga ttatcatgct gaccgggaaa
     3361 gatacagagg tagataaaat tacagggtta acaatcggcg cggatgatta tataacgaag
     3421 ccctttcgcc cactggagtt aattgctcgg gtaaaggccc agttgcgccg atacaaaaaa
     3481 ttcagtggag taaaggagca gaacgaaaat gttatcgtcc actccggcct tgtcattaat
     3541 gttaacaccc atgagtgtta tctgaacgag aagcagttat cccttactcc caccgagttt
     3601 tcaatactgc gaatcctctg tgaaaacaag gggaatgtgg ttagctccga gctgctattt
     3661 catgagatat ggggcgacga atatttcagc aagagcaaca acaccatcac cgtgcatatc
     3721 cggcatttgc gcgaaaaaat gaacgacacc attgataatc cgaaatatat aaaaacggta
     3781 tggggggttg gttataaaat tgaaaaataa aaaaaacgac tattccaaac tagaacgaaa
     3841 actttacatg tatatcgttg caattgttgt ggtagcaatt gtattcgtgt tgtatattcg
     3901 ttcaatgatc cgagggaaac ttggggattg gatcttaagt attttggaaa acaaatatga
     3961 cttaaatcac ctggacgcga tgaaattata tcaatattcc atacggaaca atatagatat
     4021 ctttatttat gtggcgattg tcattagtat tcttattcta tgtcgcgtca tgctttcaaa
     4081 attcgcaaaa tactttgacg agataaatac cggcattgat gtacttattc agaacgaaga
     4141 taaacaaatt gagctttctg cggaaatgga tgttatggaa caaaagctca acacattaaa
     4201 acggactctg gaaaagcgag agcaggatgc aaagctggcc gaacaaagaa aaaatgacgt
     4261 tgttatgtac ttggcgcacg atattaaaac gccccttaca tccattatcg gttatttgag
     4321 cctgcttgac gaggctccag acatgccggt agatcaaaag gcaaagtatg tgcatatcac
     4381 gttggacaaa gcgtatcgac tcgaacagct aatcgacgag ttttttgaga ttacacggta
     4441 taacctacaa acgataacgc taacaaaaac gcacatagac ctatactata tgctggtgca
     4501 gatgaccgat gaattttatc ctcagctttc cgcacatgga aaacaggcgg ttattcacgc
     4561 ccccgaggat ctgaccgtgt ccggcgaccc tgataaactc gcgagagtct ttaacaacat
     4621 tttgaaaaac gccgctgcat acagtgagga taacagcatc attgacatta ccgcgggcct
     4681 ctccggggat gtggtgtcaa tcgaattcaa gaacactgga agcatcccaa aagataagct
     4741 agctgccata tttgaaaagt tctataggct ggacaatgct cgttcttccg atacgggtgg
     4801 cgcgggactt ggattggcga ttgcaaaaga aattattgtt cagcatggag ggcagattta
     4861 cgcggaaagc aatgataact atacgacgtt tagggtagag cttccagcga tgccagactt
     4921 ggttgataaa aggaggtcct aagagatgta tataattttg gctctttgtc aaatgatgtt
     4981 ggtaaagaga aatct
//
LOCUS       DASHRQ010000215         3034 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.guided.148, whole genome shotgun sequence.
ACCESSION   DASHRQ010000215 DASHRQ010000000
VERSION     DASHRQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.guided.148"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            245..1213
                     /locus_tag="U2M88_003047"
     CDS             245..1213
                     /locus_tag="U2M88_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEM8375335.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="U2M88_003048"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="U2M88_003048"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEM8375336.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="U2M88_003049"
     CDS             2243..2851
                     /locus_tag="U2M88_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HEM8375337.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          903 a          581 c          729 g          821 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga ggttctgttg caaagtttta aataaagaat aaactccctt
     3001 acggtatcta tgatttaagc tgggattccc aata
//
LOCUS       DASHRQ010000216         2565 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.guided.156, whole genome shotgun sequence.
ACCESSION   DASHRQ010000216 DASHRQ010000000
VERSION     DASHRQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.guided.156"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(39..341)
                     /locus_tag="U2M88_003050"
                     /pseudo
     CDS             complement(39..341)
                     /locus_tag="U2M88_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1055..1345)
                     /locus_tag="U2M88_003051"
     CDS             complement(1055..1345)
                     /locus_tag="U2M88_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM8375338.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1553..2244)
                     /locus_tag="U2M88_003052"
                     /pseudo
     CDS             complement(1553..2244)
                     /locus_tag="U2M88_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          759 a          472 c          356 g          978 t
ORIGIN      
        1 atggattttt tcatttacac aaaatatttt atactctcaa catacttgca aagtaatact
       61 acaattccta acataatcgt agaatttaat aatttccata gcataaagtg tttagaaaag
      121 aacattaaga atagttctat aataattctt gaactccatg tcttataatg ttctatcgaa
      181 gcacccaaat agccaagttc atctaatcta cttgcataaa ctacgtcatc cgctctcata
      241 gtcggcatat acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc
      301 agttctttgt tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata
      361 tttcctaata gagtataaca tagctttaag cattacttaa agataactta aaagttatct
      421 tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca
      481 aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta
      541 atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac
      601 gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca
      661 ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac
      721 ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc
      781 tgagactagt aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact
      841 aatagggctt cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa
      901 agtttagtga ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt
      961 gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag
     1021 cggctaggac atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga
     1081 ccttttttct tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca
     1141 catgatagga tctcacaaca aattgctttc tattattata ataacctgta acgactaact
     1201 tcatcccttc gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga
     1261 ccccatttaa actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat
     1321 ctactgttcc atattttttc tccataacct tcacctcaat accattatac gaacatacat
     1381 tccctttgta aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt
     1441 aaaatcatat tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca
     1501 gtatagatta ctgttgcttc tttactaatt taggtttact tgttaagaaa gattatttac
     1561 gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt atcttccact tcatcttttg
     1621 ctttttctaa cttttgaata tattgtttca agcttgagtt ttcagcagtt gcttcgtcta
     1681 atttcttttg tgtatctgtt aactgatttc tacatttgtt ttttcttgtg tcagtgttgc
     1741 gatttgttga ttcaaaccat ttacagtatt ttgtaattct gaaagttttg cttgtgcttc
     1801 ttgtagtctt gctgtccaat ctgcattcag atgattgatt tcatcatttt tttggttgat
     1861 ctcatctagt ttgacattga tttcattttg tttgctttgt agttgtgcat ccttgctgac
     1921 taattgactt tctaagtttt gtttttctgc tgccgattga tttttttggt tttctaattc
     1981 atttactttt gtttggtatt cttggacatc tgcctttgaa tcttgcaatg tctgttcttt
     2041 ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct ttatctgtca atgtatgatc
     2101 aagctgtgtc agattctcat tgattgtatc aatatctgtc tctccacccc acaaaataat
     2161 ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat agaaatgcaa cgacaatccc
     2221 accagtaata aatttctttt tcatttgcat agctccttaa tccaatactt ttacatgcta
     2281 aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa ttacatatgt aattttaata
     2341 taaatactat caaatactca tttaatcctc cttttttatc tatttaaaaa atctttttta
     2401 tagagaaaaa tgcaggttaa tactttctta aataaatatt tatggtgaac aaaaacaaaa
     2461 aagcaagttt tattaaacgt aacccttttc atttcataga ttactgtatt tattacaaaa
     2521 aacgcggatt tgtcataaat tgtcatgctt cttttaccta tgaaa
//
LOCUS       DASHRQ010000217         1531 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38041
            SAMN38764656-rid20545813.guided.173, whole genome shotgun sequence.
ACCESSION   DASHRQ010000217 DASHRQ010000000
VERSION     DASHRQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764656
            Sequence Read Archive: SRR27176885
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 11-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024283.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024283.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 05:26:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,053
            CDSs (total)                      :: 2,996
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 37 of 76
            Pseudo Genes (incomplete)         :: 27 of 76
            Pseudo Genes (internal stop)      :: 22 of 76
            Pseudo Genes (multiple problems)  :: 9 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764656-rid20545813.guided.173"
                     /strain="VRE38041"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2022"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            364..1275
                     /locus_tag="U2M88_003053"
     CDS             364..1275
                     /locus_tag="U2M88_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HEM8375339.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          544 a          218 c          316 g          453 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccatt gaagatgtaa ggcagagcga
       61 tattgcggtc attatctgcg tgcgctgcgg caagatagcc tgataataag actgatcgca
      121 tagaggggtg gtatttcaca ccgcccattg tcaacaggca gttcagcctc gttaaattca
      181 gcatgggtat cacttatgaa aattcatcta cattggtgat aatagtaaat ccagtagggc
      241 gaaataattg actgtaattt acggggcaaa acggcacaat ctcaaacgag attgtgccgt
      301 ttaaggggaa gattctagaa atatttcata cttccaacta tatagttaag gaggagactg
      361 aaaatgaaga agttgttttt tttattgtta ttgttattct taatatactt aggttatgac
      421 tacgttaatg aagcactgtt ttctcaggaa aaagtcgaat ttcaaaatta tgatcaaaat
      481 cccaaagaac atttagaaaa tagtgggact tctgaaaata cccaagagaa aacaattaca
      541 gaagaacagg tttatcaagg aaatctgcta ttaatcaata gtaaatatcc tgttcgccaa
      601 gaaagtgtga agtcagatat cgtgaattta tctaaacatg acgaattaat aaatggatac
      661 gggttgcttg atagtaatat ttatatgtca aaagaaatag cacaaaaatt ttcagagatg
      721 gtcaatgatg ctgtaaaggg tggcgttagt cattttatta ttaatagtgg ctatcgagac
      781 tttgatgagc aaagtgtgct ttaccaagaa atgggggctg agtatgcctt accagcaggt
      841 tatagtgagc ataattcagg tttatcacta gatgtaggat caagcttgac gaaaatggaa
      901 cgagcccctg aaggaaagtg gatagaagaa aatgcttgga aatacgggtt cattttacgt
      961 tatccagagg acaaaacaga gttaacagga attcaatatg aaccatggca tattcgctat
     1021 gttggtttac cacatagtgc gattatgaaa gaaaagaatt tcgttctcga ggaatatatg
     1081 gattacctaa aagaagaaaa aaccatttct gttagtgtaa atggggaaaa atatgagatc
     1141 ttttattatc ctgttactaa aaataccacc attcatgtgc cgactaatct tcgttatgag
     1201 atatcaggaa acaatataga cggtgtaatt gtgacagtgt ttcccggatc aacacatact
     1261 aattcaagga ggtaaggatg gcggaatgaa accaacgaaa ttaatgaaca gcattattgt
     1321 actagcactt ttggggtaac gttagctttt taatttaaaa cccacgttaa ctaggacatt
     1381 gctatactaa tgatacaact taaacaaaag aattagagga aattatattg ggaaaaatat
     1441 tatctagagg attgctagct ttatatttag tgacactaat ctggttagtg ttattggttc
     1501 tgttgcaaag ttttaaataa agaataaaat c
//
